LOCUS       DAXDSD010000001        40701 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXDSD010000001 DAXDSD010000000
VERSION     DAXDSD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40701
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.001"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            69..1193
                     /locus_tag="ACFB2Q_000001"
     CDS             69..1193
                     /locus_tag="ACFB2Q_000001"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC0917134.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
     gene            1190..2227
                     /locus_tag="ACFB2Q_000002"
     CDS             1190..2227
                     /locus_tag="ACFB2Q_000002"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="HFC0917135.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDITSQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            2243..4117
                     /locus_tag="ACFB2Q_000003"
     CDS             2243..4117
                     /locus_tag="ACFB2Q_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HFC0917136.1"
                     /translation="MNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLGNVQSG
                     KTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEADDRSFM
                     EVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTNSDKPA
                     KDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQPEFIHFFEAGEKYIGG
                     NFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKANCNFA
                     LHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIHHFDEI
                     YEKLTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTVYYSRT
                     AKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKNSGGNI
                     QVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKDEIQSD
                     LYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGRKLSRA
                     TGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLVYQSAK
                     "
     gene            complement(4180..5244)
                     /gene="hemE"
                     /locus_tag="ACFB2Q_000004"
     CDS             complement(4180..5244)
                     /gene="hemE"
                     /locus_tag="ACFB2Q_000004"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC0917137.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFAHA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMVQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(5319..6536)
                     /locus_tag="ACFB2Q_000005"
     CDS             complement(5319..6536)
                     /locus_tag="ACFB2Q_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC0917138.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPENMRRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            complement(6533..7867)
                     /locus_tag="ACFB2Q_000006"
     CDS             complement(6533..7867)
                     /locus_tag="ACFB2Q_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC0917139.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            complement(7879..8643)
                     /locus_tag="ACFB2Q_000007"
     CDS             complement(7879..8643)
                     /locus_tag="ACFB2Q_000007"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC0917140.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPTAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            complement(8661..9083)
                     /locus_tag="ACFB2Q_000008"
     CDS             complement(8661..9083)
                     /locus_tag="ACFB2Q_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC0917141.1"
                     /translation="MKKMFLPAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            complement(9365..10333)
                     /locus_tag="ACFB2Q_000009"
     CDS             complement(9365..10333)
                     /locus_tag="ACFB2Q_000009"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC0917142.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVTEPKLKEPLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            complement(10333..11157)
                     /locus_tag="ACFB2Q_000010"
     CDS             complement(10333..11157)
                     /locus_tag="ACFB2Q_000010"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFC0917143.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            complement(11187..11828)
                     /locus_tag="ACFB2Q_000011"
                     /pseudo
     CDS             complement(11187..11828)
                     /locus_tag="ACFB2Q_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(11997..12317)
                     /locus_tag="ACFB2Q_000012"
     CDS             complement(11997..12317)
                     /locus_tag="ACFB2Q_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC0917144.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            complement(12340..12966)
                     /gene="upp"
                     /locus_tag="ACFB2Q_000013"
     CDS             complement(12340..12966)
                     /gene="upp"
                     /locus_tag="ACFB2Q_000013"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC0917145.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(13072..13383)
                     /gene="grxD"
                     /locus_tag="ACFB2Q_000014"
     CDS             complement(13072..13383)
                     /gene="grxD"
                     /locus_tag="ACFB2Q_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC0917146.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(13484..13699)
                     /locus_tag="ACFB2Q_000015"
     CDS             complement(13484..13699)
                     /locus_tag="ACFB2Q_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFC0917147.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYCRSQH"
     gene            complement(13725..14504)
                     /locus_tag="ACFB2Q_000016"
     CDS             complement(13725..14504)
                     /locus_tag="ACFB2Q_000016"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC0917148.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAVAEAHEHIYCTIGVHPDSKEAEEFSIAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(14509..14859)
                     /locus_tag="ACFB2Q_000017"
     CDS             complement(14509..14859)
                     /locus_tag="ACFB2Q_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC0917149.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            complement(14863..15840)
                     /locus_tag="ACFB2Q_000018"
     CDS             complement(14863..15840)
                     /locus_tag="ACFB2Q_000018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC0917150.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKKVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            complement(15874..17004)
                     /locus_tag="ACFB2Q_000019"
     CDS             complement(15874..17004)
                     /locus_tag="ACFB2Q_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC0917151.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL"
     gene            17172..17873
                     /locus_tag="ACFB2Q_000020"
     CDS             17172..17873
                     /locus_tag="ACFB2Q_000020"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC0917152.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFEEFLKTA
                     AASSAKMVAEIVKSL"
     gene            18015..19808
                     /gene="lepA"
                     /locus_tag="ACFB2Q_000021"
     CDS             18015..19808
                     /gene="lepA"
                     /locus_tag="ACFB2Q_000021"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC0917153.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            19954..20973
                     /gene="lepB"
                     /locus_tag="ACFB2Q_000022"
     CDS             19954..20973
                     /gene="lepB"
                     /locus_tag="ACFB2Q_000022"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC0917154.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSGDSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(21078..21185)
                     /locus_tag="ACFB2Q_000023"
                     /pseudo
     CDS             complement(21078..21185)
                     /locus_tag="ACFB2Q_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(21409..21558)
                     /locus_tag="ACFB2Q_000024"
     CDS             complement(21409..21558)
                     /locus_tag="ACFB2Q_000024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917155.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(21604..22511)
                     /locus_tag="ACFB2Q_000025"
                     /pseudo
     CDS             complement(21604..22511)
                     /locus_tag="ACFB2Q_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(22820..23752)
                     /gene="cysK"
                     /locus_tag="ACFB2Q_000026"
     CDS             complement(22820..23752)
                     /gene="cysK"
                     /locus_tag="ACFB2Q_000026"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC0917156.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTEIYDSITKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(23948..24553)
                     /locus_tag="ACFB2Q_000027"
     CDS             complement(23948..24553)
                     /locus_tag="ACFB2Q_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917157.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            complement(24550..25401)
                     /gene="dapF"
                     /locus_tag="ACFB2Q_000028"
     CDS             complement(24550..25401)
                     /gene="dapF"
                     /locus_tag="ACFB2Q_000028"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC0917158.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVPIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            25538..25852
                     /locus_tag="ACFB2Q_000029"
     CDS             25538..25852
                     /locus_tag="ACFB2Q_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917159.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNALVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            25849..26517
                     /locus_tag="ACFB2Q_000030"
     CDS             25849..26517
                     /locus_tag="ACFB2Q_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC0917160.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(26590..28713)
                     /gene="pnp"
                     /locus_tag="ACFB2Q_000031"
     CDS             complement(26590..28713)
                     /gene="pnp"
                     /locus_tag="ACFB2Q_000031"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC0917161.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKRLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(28947..29810)
                     /locus_tag="ACFB2Q_000032"
     CDS             complement(28947..29810)
                     /locus_tag="ACFB2Q_000032"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC0917162.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPV
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEEGTLTLMDE
                     VLTPDSSRFWPADQYEVGINPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            30003..30866
                     /gene="rfbD"
                     /locus_tag="ACFB2Q_000033"
     CDS             30003..30866
                     /gene="rfbD"
                     /locus_tag="ACFB2Q_000033"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC0917163.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPHQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(31237..31470)
                     /locus_tag="ACFB2Q_000034"
     CDS             complement(31237..31470)
                     /locus_tag="ACFB2Q_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC0917164.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(31488..31622)
                     /locus_tag="ACFB2Q_000035"
     CDS             complement(31488..31622)
                     /locus_tag="ACFB2Q_000035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917165.1"
                     /translation="MPSELFRRHFCCFWQDLSIIFLEDIVFSQIDHDSAASKALACAN
                     "
     gene            complement(31636..31836)
                     /locus_tag="ACFB2Q_000036"
     CDS             complement(31636..31836)
                     /locus_tag="ACFB2Q_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC0917166.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(32008..32883)
                     /gene="xerD"
                     /locus_tag="ACFB2Q_000037"
     CDS             complement(32008..32883)
                     /gene="xerD"
                     /locus_tag="ACFB2Q_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC0917167.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            complement(32935..33375)
                     /locus_tag="ACFB2Q_000038"
     CDS             complement(32935..33375)
                     /locus_tag="ACFB2Q_000038"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC0917168.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            33696..34634
                     /locus_tag="ACFB2Q_000039"
     CDS             33696..34634
                     /locus_tag="ACFB2Q_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC0917169.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            34763..35056
                     /gene="hfq"
                     /locus_tag="ACFB2Q_000040"
     CDS             34763..35056
                     /gene="hfq"
                     /locus_tag="ACFB2Q_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC0917170.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            35141..35707
                     /locus_tag="ACFB2Q_000041"
                     /pseudo
     CDS             35141..35707
                     /locus_tag="ACFB2Q_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            35761..36135
                     /locus_tag="ACFB2Q_000042"
     CDS             35761..36135
                     /locus_tag="ACFB2Q_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC0917171.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            36145..36639
                     /gene="folK"
                     /locus_tag="ACFB2Q_000043"
     CDS             36145..36639
                     /gene="folK"
                     /locus_tag="ACFB2Q_000043"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC0917172.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(36767..37246)
                     /locus_tag="ACFB2Q_000044"
     CDS             complement(36767..37246)
                     /locus_tag="ACFB2Q_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC0917173.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAHKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(37466..38203)
                     /gene="ubiE"
                     /locus_tag="ACFB2Q_000045"
     CDS             complement(37466..38203)
                     /gene="ubiE"
                     /locus_tag="ACFB2Q_000045"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC0917174.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(38246..38614)
                     /locus_tag="ACFB2Q_000046"
     CDS             complement(38246..38614)
                     /locus_tag="ACFB2Q_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC0917175.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            38734..40353
                     /gene="mnmC"
                     /locus_tag="ACFB2Q_000047"
     CDS             38734..40353
                     /gene="mnmC"
                     /locus_tag="ACFB2Q_000047"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC0917176.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
     gene            complement(40350..40499)
                     /locus_tag="ACFB2Q_000048"
     CDS             complement(40350..40499)
                     /locus_tag="ACFB2Q_000048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917177.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT        10336 a        10954 c         9355 g        10056 t
ORIGIN      
        1 agagcatagt tcacctttaa aatccataca aggattttta tcaaatttat gcataggagt
       61 gaaatcctat gctatctaaa caaatctcaa atcttaattc ttctagtaac aaaccaaaaa
      121 tcctatctct attttcagga tgtggcggtt tggatttggg ctttcatcaa gctggttgtg
      181 aaactgtttg ggcgaacgat ttctcccatt gggcttgcga aagtttccgt aaaaatatcg
      241 gcgatgtcat cgtagaaggt gatattgaac aaattaatcc gaatgatcca actattcccg
      301 attgcgacat cattttaggc ggattccctt gtcaagattt ttccatgatt tggaaacagc
      361 cgggcttaga gggtgagcgc ggcaatcttt ataaaagctt tttacgtttt gtaaatgcaa
      421 aaaaaccgaa agtttttgtt gctgagaatg tgaaaggttt attgactgcc aacaagaaaa
      481 aagccatcca gcaaattatt accgactttg aaaattgcgg ttattacgtt caggcgaagc
      541 tgtataactt tgcagaattt ggcgtacctc aatttcgtga acgtgtgctg attgtcggag
      601 tacgtttgga tacaggattt gattttcgcc atccggaacc gacgcacaat gaaactggcg
      661 aaaacggctt aaaaccatat gtaacagcag gtcaggccat atccaatatt ccacaaaatg
      721 ccagtaataa tgaattactg aaaatcagcg ataaaacacg ccgtatgttg gaattaattc
      781 ctgaaggtgg aaattttacc gatattccta aagatcatcc tttatatgtg aaaggtatga
      841 ttagccacgt ttatcgtcgt atgcatcgga acgagccatc aaaaacaatt attgcagcag
      901 gtggcggtgg tacttggggc tatcacttcc ctgaaccgcg tgcttttact aatagagaac
      961 gagcaaggct tcaaagtttt cctgatgatt ttgagtttgt cggatcaaca actgaagtac
     1021 gtcgccagat tggtaatgct gttcctcctc agggcgtggt tgaactggca aaaagcattt
     1081 taccgatttt ttcagacaac tatgagaaag tagatttgca tgagaaatta gtcgaagaaa
     1141 aagaaatttt attccatgac cgactaagca aaattcgagg gggaaaacaa tgaatacagt
     1201 tttttccaat atcgccaatg ccaaaatcac tgaaaaatct cttaatgcgg tttggatgga
     1261 tttatttaaa tcagcggatg aagtgttgat ggcgacaggt tatgtatcta atgacgcagt
     1321 ggttgagtta cataaaattt tggagctaaa cgatcatatt caaaaaatag atttattggt
     1381 cggaatgcat tatttggaag gttttagcca tttacaatat gacagcttat gtaaactaaa
     1441 tgattttttg cgacatgaga aaagaggagc cgtctatgtt tccccctttg tgaaatttca
     1501 cggcaaaatg tattccttta aaaattatca aaaaatcaat ggattaattg gttctgctaa
     1561 cttaacgtgt ttttgggata gcacggaacg tacttatgaa actatgttgc atttgaacgg
     1621 taagcctgca cagattttgc aggcagatat tcaaagtact attcataagc taggcaaaaa
     1681 tatccaagaa gttgaaagac caagtaaatt tattgaacat aatagccatt tagaaaattg
     1741 tttgggtgtt cagaaaattg caccagaaca gattcggcaa ttatttgccc aaacgtcaga
     1801 atatcatttc tcaattccag ctaaaaccga agaaaaaagt aatttgaatg tattttttgg
     1861 agaaggtcga cgagataaac gagggtttgt caaaccacga ccttggtatg aagtagagtt
     1921 gattgtatcc aaagacatca cttcacaaga agggtatccg gttctgaagt cttttacagt
     1981 tattaccgat gatggttggc aatttcaatg caagacatcg ggggattact ccaaaaactt
     2041 ccgctcagaa aatgatttaa agacacttgg taaatggatt aaaggtcgtt tggaaagtca
     2101 tggctgcctg caaaataacg agaagattac gcatgaaacc ctacgcgaat acggtaacga
     2161 tcattttgag ttacgttcaa ccgataatcc tgatgtttgg ctgctctcat ttaaggggaa
     2221 aaactaaatg ttgcgaacct acttgaacca attgacccct cccgaattag ctgattctgt
     2281 taaaaatacc gttgatggtt ttatggaaaa attgtcacaa accgagccga aaatcgccca
     2341 aaatgtttta ttgctgggca atgttcaaag tggcaaaacg gcacaggttt tgggtgtatt
     2401 aagtgcgctg gctgatgatg gcgaccacaa agtttttttg tatcttacta ctgacagcgt
     2461 agatttacag gatcaaacag tcaaacgagc caaagccaat ctgaaaaact ttatcgtatt
     2521 gtctgaagct gacgaccgaa gttttatgga agtaatgaag gcagagaacc ccatcttggt
     2581 cgtgattaaa aagaatgccc gtgtattgaa acgttggcgt aatttgtttg ccagtcaaag
     2641 tagcctgaaa ggctatcctt tggttattgt ggacgatgaa gccgatgctg ccagcctaaa
     2701 tactaattct gataagccgg ctaaagatgc cagtactatc aataaactgc taaacgacat
     2761 caaaaattcc tgttgtcaaa gcctgtttat ccagttgact gccacgcctc aatcgctttt
     2821 attacaacat gaagaatctg attggcaacc tgaatttatc cacttctttg aagcaggcga
     2881 aaaatacatc ggcggcaact ttgtcttttc tgatccacca agctatatag ttcgttttat
     2941 tgatagcgaa ttggatgata tgaaagacga gagtggtgaa attgccgaag gagcaaaaca
     3001 ggcattgctt agttttctaa taacctgtgc agaattcgcg ctatgtgata aggctaactg
     3061 taattttgca ctacatccaa gctataaaat tcaagatcat caagcctttt caaagaagat
     3121 ccaagccttt ttaaatgact tagtgcaagc agtcaataat ggggaagacc ttgcaggtag
     3181 ctttaaagaa agttatttgg atttgcaaaa gaccaagccc gatattcatc actttgatga
     3241 aatatacgaa aaattgacag cacttttaga gaataaacaa atctctactc ttgtcgttaa
     3301 ctcgcagaca gaaacagatt ttgacttaga aaaaggtttc aacatcatta ttggtggtaa
     3361 tgtgattggt cgtggcttga ctattccgaa actacaaacg gtttattata gtcgcactgc
     3421 caaaaaaccg aatgcagata ctttctggca acattcgcgt atttttggat atgaccgcga
     3481 taaatcatta ttacggcttt atattccgtt tgatgtctat tacttctttg tacaactcaa
     3541 tcaagcaaat aatctgatta ttgggcaggc aaaaaattca ggcggcaata ttcaggttat
     3601 ttatccgaaa aatatcaatc caactcgaaa aaatgtattg aaattcgata gtatcaatca
     3661 aattgtgggt ggtgtgaatt attttccact ccacccaaac gaagataatt tatcggagat
     3721 taacaaaatt ttgccttcta ttttgaaaga tgaaattcaa tctgatttat atcaaataga
     3781 tatagaagat ttattcttgg ttctagataa attgggtcgt tatgttccag atgactggaa
     3841 taaggaaaaa tttattgctg gtgtagaagc attaaaagca caacgtccaa gttttaaaac
     3901 ctatgttttg atcaaaaccg gacgtaaact ttctcgagca accggcacaa tgctttcgga
     3961 agacgaccgt aaattaggag aaaaatatcc caatgattta ttccttacgc tttatcaagt
     4021 agtaggaaat aaagacaaag gttggcaggg aaaagatttt tggttaccca atatcaagct
     4081 accacacaat ggcttggtgt accaaagtgc aaaatgaagc atatgccgtc tgaaaacttc
     4141 cgattcttca gacggcatat cgggcggttt cctgccggct tacccgccgt gatactgccg
     4201 cgacagctcg tgtacggtgt cgactaagat tttggcgtgt tcggggtcgg cgtgttggtt
     4261 gatgccgtgt ccgaggttga agacatggcc gctgccgtgt ccgtagccgg tcaggatacg
     4321 tgcgacctcg gtgcggatgg attcgggcgt accgaagagg gcggacgggt cgaagttgcc
     4381 ttgcagggcg acttggttgc cgacgcggcg gcgtgcttcg ccgatgttgc acgtccagtc
     4441 caagcccaat gcgtctgcgc cgatttggac catgctttcc agccacagcc cgccgccttt
     4501 ggcaaacacg ataacaggca cgcggcgacc ttcgctttca cgtttgagtc cggcaacgat
     4561 ttggcggatg tatttgaggc tgaactcttt aaacgccgcg tcgctcaaca cgccgcccca
     4621 agtgtcgaaa atctgcaccg cctgcgcgcc cgcgtcgatt tgggcgttga ggtaggcggt
     4681 aacggcttgg gcgttggtat cgaggatttt gtacagcaaa tcggggcgcg agtacatcat
     4741 ggttttgatg gtgcggaatt ctttgctgcc gccgccttcg accatataac aggcgagcgt
     4801 gaacgggctg ccggagaagc cgataagcgg tacgcggccg tccaatgctt tacggatgga
     4861 agttaccgcg tcgaaaacgt attgcagttt ttccatgtcg ggaacgtgca gcttggcgat
     4921 gtcggattcg tgttgcaagg cgtgtgcgaa tttcgggcct tcgccttcgg caaaatacag
     4981 ccccaagccc attgcatcgg gaacggtcaa gatgtcggaa aacaaaatcg ccgcgtccaa
     5041 atcgaagcgt tccaaaggct ggatggtgac ttcggtcgcc aatccggtgt ttttgcacaa
     5101 atcgaggaag ctgcccgctt tcgcgcgtgt ggctttgtat tcgggcagat aacgccccgc
     5161 ctggcgcatc atccaaatcg gtgtgtattc gacgggctgt ttgagcaggg cgcggaggaa
     5221 ggtatcgttt tttaaaagag tcatacgggt tttctgtcag tctgtttgtc aagtgaaatg
     5281 gttttcagac ggcatttcaa cgatgccgtc tgaaggtttc aacgggtttc ggcggaaggg
     5341 cggttttccc cggcaacgct tgccaaaacc aagttgcgct gtgcttcggc tttttgcgac
     5401 tgtgcggttt cgtcaaaaac ctttgccaac accaaacgcg ccggaatact cggcttcagt
     5461 gcaatactcg cttcaaggta gccttttgcc ttaccccaaa gtttgcggcc gtaggcgagc
     5521 cggccgagat acatcagcag aagcgcgtta tcgggctgtt ctttcagcca agaatcggca
     5581 aaatcgatgg ctttctgctg ttcgcgctcg cccaaaaagc gcacgctttc gacaaaggct
     5641 tccaaaagct cggggcggcg gttgtgcgga taatgctgtt tgacccattt gaccgcatcg
     5701 gcatacagtc ccaaacgttc gtacttttcc gcaaccgata cgctcaattc cccgtttttg
     5761 aggctgtcgg gaatccgctt caggcaggtt ttcaaagcgg cggcatcggc agcatccgcc
     5821 atctggcggc ggtatgccca attttgatac cgttccattt ccgatttgcc caacgcgccc
     5881 gccttggaaa gtttttcggt ttttgccaga acctgcaacg catcgccccg atcgaaggcg
     5941 taacgaagtt gcagacgcac gaggcgcgta aggttggcat tcatcttcgc cgccgcatga
     6001 agattggctt ccgccgcttc gtaatcgcgc cggtttaacg ccgattccgc cagcagaaga
     6061 tagcgggaaa gctgctgttt ttccggcagt ttggcgattt ccgcaagata acggtcgcgc
     6121 agctcgatat tttccatctg tcctgccgcg tgcgcgccca gcatcaatgc caaagtccgg
     6181 ttgtctccgg cctctttgtt gcccaacact cgagaggctt cgagttccgc cttttcaaaa
     6241 cgcccttcga aatacgccaa acccgcctta ttcaaggcaa gcgcggcctt gcggcctttc
     6301 cgcgccgaac cggaacgccg catattttcg gggatattga gtacgccgat gatgaattta
     6361 aacaggaaat accacaccac gacggcaatc agcgaaccta acacaaaggc gtgcaggttg
     6421 attctgagca tggtctgtcc gagtacgata tacacgtcgc cggtgtaaat gcccgaagcc
     6481 agcgccagtc cgacggcggc ggcaaacagg acaacaatcc agactaccgt tttcatgcgc
     6541 gttctccttt agcggacggc gaacgccctg ccttttccgg tgcgggctgt ttttttgcag
     6601 gctgttccgg tttgcgttcc gaaggcaggg acggtgcttc tatggccttc acatccgatg
     6661 cggcagccgg ttcggaagct gtgttttcgt ttgccggttc ggaagccgcc tgttccgctt
     6721 cttgattttc cgccgccgtc atgcgcgtac cgtcgcgata ggcgcggacg gcatttaaac
     6781 tggtcttcaa gccgtcatcc gccgtcatgc gtacatccag tgttttcaat tccgccagtt
     6841 ctttcagcca cgactgcgtg gcgggcgact tggcatcgaa atactgtctg acggcggctt
     6901 cggcattgtt caaatcgccc tgatagactt cgccgttgcg ctgcatcaat gcggtgcgcg
     6961 catccaacag gcgcagacgc aggttttcac gcacaaaata tgcctgttcg ggagaaatca
     7021 gcatggcatc gttgttttcc aaacggcgga tttcgaccag ccccttcaat gtgccgaggg
     7081 atttttccca tacgttctgc caccatgaag cggaaacggc ttcgttgcgg gcctgtacgc
     7141 ccggtttcaa tacgccgtcc aaaatcagcg gcagtccgga tacggcggtt tccaaccggt
     7201 cgaggcgcaa tgccgtgccg gaaatatcga catagggacg gtttttcagt tccgccaaat
     7261 cgctgctgac cgcctgtttg atcggcagaa gctctgcctg atcgaaacgg gacaggcggc
     7321 tgtcgatatg ctccaacacg ccgactgccg tttggatatt gccggtcaac accagctgtt
     7381 gcgccgccag attgagtatg gtctcggttt cgtccaccag ccaatcggca cgccctttgg
     7441 tcagctcgcg ataggctttt tgcgtcatca ggatttgttc gccgttggct ttgacaccgc
     7501 tgtccaaacg gtcgagttcg gactgtatgg cactttggcg gttgaggttg tctttgagca
     7561 gggcggcgtt ttccgactcg cccaaggcgg ctttgtcgat tttctggttg aatgccagct
     7621 cttggttttt caagacattc tgtccttgga caaacaaaaa accacttgca cccaaaccca
     7681 atgccgccaa taccaaggcg caggccgcca aagcgttgct gccggactgt ttgacgataa
     7741 aaggagcggc gggaatgtgt acttctgttt cgttttcttt gcgtggggaa gaggtttcag
     7801 acggcatttc ttttgatgcc tgtatctcgc gtacgggttc ggatgatttg ttttcaggtt
     7861 cgcccaccgt tttgctcctt attggtttga ggttccggga aagaccatgc cgtctgaaac
     7921 ggaaatggaa gaataggaaa gcgcggcttc cagcgtaggg acggtttcga ccgaacacac
     7981 gccttcgcgc ttcaatgcct ccgcaatgcg cggatgatgg gtaaagtata gcaaggattt
     8041 gaagaatcgg gaaaattgcg gcggaagctg tgcaaacagc gactgcacaa gttctgtgga
     8101 cgtaatatag gcggcggcaa tattttcggt ttggaaattt tgaaagttca aaggtttatg
     8161 ccgtctgaaa tagacttctg ccacctccgt ccgaaaacct ttctcctgca aggcattcat
     8221 cagaaaatcc cgcccgccgt gtccgcgcac agacaatacg cgcgcacctt cgggcagact
     8281 gttccaaacc ggcaggcgca aaaccgcctc gctgtcgttg ccgtcttcag gtgcaaacac
     8341 gcccgcgccc gcataacgcg ccaaggcacg gcggctgcct tgccctacgg caatgtgcgc
     8401 ctttatgccg tctgaaaggt taaggtacgg gacggcggtt tcaacggcgg tcggactgac
     8461 ccaaaacacg gcatccgcac gggcatacat ttccggcagc cgtttcaggc ttgcttcgtc
     8521 ggtttcgatt tcaatcggac tcaacacttc cgcctgccat ccggcacgac ggcagacttc
     8581 cacatcgtcc ttcgccctgc ccgacgggcg gacaatcagc atgacgggct tcgctgtttc
     8641 catcgcctta tccgccccga ttaccgggaa aaatcgtcat cgttgaaatt cgcctgcgcg
     8701 gttttcggcg tacagcctac ccgtgcgact tccttgcccc tacgctccac aacgacaccg
     8761 ctgctttccg tatgagtcac ggaatcgaag cctgtccata cggtgtacat aaattcgccg
     8821 ttttggaatt tcatcgttgc ccggcgtccg ctgcccatgc cttgccacct gtcggaacgc
     8881 cccaacaggt cagctttgct gttgcgtatg gcaatttctt tttttgccgc actgccgaac
     8941 gaatattcgt aaagattgcg gttgattttt tgaacttcta tgtatttgtt gttgtccgtt
     9001 ttacaggaaa ataccgtatc cgcccacacg gtttgggcgg cagccgacag aagcaatgcg
     9061 gcaggaagga acattttttt catcataatt tccttaatgg ttgaaacccc gccattcgga
     9121 catccgtcct tcgtggcagc agcatcagat tttatttgaa aggggtgtaa cccctttcaa
     9181 attagggcaa cacatagggc ggcgctttat gtgtcgtcct gtgtgttgaa acatcattgc
     9241 ccgtatcctg aaatagaagg ctgtgtttta acaatccgtt gattgcttaa gatgaattca
     9301 cactaaaaaa cagttctcta tttcatctat tacggcgggt tgttttgttt ataagggtgg
     9361 taatctacaa attgttacta aatagcaagg tgtgaatgaa acggcagata taatcaaaat
     9421 cttcttgttg gtcaaaatac ggtaacataa tgctttttaa tttttcgcct ttgggtttac
     9481 gtccataaga aaacagatag cggtttgctg taatacattt agcgtaatac agttttactt
     9541 ctattggcat atcggactgt atgggtgtta atacggcgac atcactattt gctacaaatt
     9601 cacaagttga aacatagcta taagtatggc taccttcgcc attggtagaa gtgaaaagcg
     9661 tatggcatgg aaaaatatgt ttttcatcaa cgctatcacg agcgatataa cttcgtagcg
     9721 ttcttgcttg cgtactggca ggacggatat agacaaccgt gtctttttgc cgttgttcaa
     9781 attcttttaa tttggttgcg gcaatgccgt tttgaatagt aaaaatttca tcaagtcgtt
     9841 ttgattgtcg aaccacaggc aaatcaagcg gttcttttaa tttcggttcg gttacacccg
     9901 cagatggatt taaattcacg ctttcaaccc agcttgggat ctcatccaaa gatggcacaa
     9961 acagttgtcg taacgtgcgg ttagcttccc taccgcaagc agaaaaacgg taggcattgg
    10021 caaaaatagc agcacaataa aagagttttt gttgttctgt catttctgtt ttgggattta
    10081 aaacagcaac attttgagct gtataaaatg gtttgttttg taaaaaagta gataaaacag
    10141 aaccacctaa tgcaacagtt aataatcctg ctggataagg ttttgtattt ttcaataaat
    10201 ccacagatgc tgttacacca ttatttttgc ctgacctgcc tacaaagttg attgttggat
    10261 tgaagctgct cattttattc aaatctaatt ttgaaccgta actcacatca aaaatctctt
    10321 gcaattcaac cattattcat cgtctccgaa gtcgttattg tcatcgtctt catagctttc
    10381 attatccaaa ttttcagtcg gccctacttc cgctcccagt agttgaaata aagcaaattc
    10441 acgcacgact tgctcaaaat ctgctcgggt aattttggaa taatcagttt ccaaataggc
    10501 ttcggcaacc cattcatcgt tatcagttac aaatgctgtt accgattctc ctgtgtgaac
    10561 ttcgttgttt aaataatgtt ccaaccagcg cgctttaatg ccctgccagc ggtcgtataa
    10621 atcaatgcgt cccaagtgtt tggttttttc aaaaccgtca tcacgccagt agccaaacca
    10681 cgttttgcga caggcttttt tcgtgctgat ttcttcttgt gtgtccgggt caatcacgac
    10741 tgtctgaaaa tgcggtttat gggcttcaaa tacgacaatg cacgttaccg tgccaactgg
    10801 gtaaaacagt tcgctcggca tagacatcac ggcttttaag cgatgatatt tcacaatttc
    10861 gcttttggct ttgcttggtg caatcacgca actgactgga ataatggcaa tgcctgtgcc
    10921 accttctgcc aacatatcca acatttcttt gacaaaataa agttcatgca attcggcatc
    10981 gcttttggat tgggcgtaag gtggattgag aaagccaaca tttgggcgtt ttaagcctgt
    11041 ttcaggtttg ggatttttga ttaaatcctg aaaatcagtc atgaaacaac tgctttggtg
    11101 taaattggct ttaccgtctc cgcgtaaaat catattactg gcggcaagag caaacatttt
    11161 tgggttgttt tcaatgccaa tcagttgata ttttttagag atggcttacc caaattgcta
    11221 attctagcaa cagatttacc gtctaaattg ataccacaag gaacacaaac ttgttctttg
    11281 gaaatgccac gtttcgttgc tctcgttcct cgtttgtgag caggacgtgg cagattaaaa
    11341 tttttgtggt gccctttgta agaaatcgtc gaataagttt catcagcttg cacaatgccg
    11401 tctaactcaa cttcattcat catgttttga agtgcgtcca aaattttgtg ccgccaagta
    11461 aacgcagtag caagattgat tttgcaaatt tcagcacatt tgcgaagtgg gtatttttca
    11521 atcatacaat gaatgtattt ttcccaaact tcaatgtctt tttgcgtgtt gtagagaata
    11581 gtgcctgtct gctcaacaaa agacttttta caatcacgac acaaaaaacg ttggttgcca
    11641 caatctttgc catttttgac gaaatgtgta gattggcaat gtgggcactt cgtaactttt
    11701 cttggttcaa tgactttttt aacctgttct tttgagctga cagaagtcaa aaaagcctgt
    11761 ttatccgtat cggacaaatt ttcaaaaagt tgttttaaaa tgtctaattc ttttgttacg
    11821 cccgacatta taaaatccct tatagttcaa tttgttataa gggattttat caaaaattag
    11881 tgcaaaaatc aacggattgt taaaacacag ccaaatagaa aagaaaatgc ccgttttacg
    11941 gtcttctttc cgacaaaatg ccgtctgaaa cgaatttcag acggcatggt cgaaaattat
    12001 tgcgcggcaa gcggtttgag caggagggtt tcgccgcttt gcacgacatt ttccagcaaa
    12061 tcgatgttcg gatgcttccc cggatgggcg cgcgcgtcgg caacatgttc ggcaaaccat
    12121 atcaaaccct gttctgccgc ttctttaccc aattttccat caaaacgttc cgccaaggca
    12181 ttgtacagct tgagcgaacc gagcttgccc tgaacggcgg gaatgtggtg aaccgttttg
    12241 ccttcggcat cctgaacatc caaaccgccc agatggtcga ttgaaggaaa tgtggcgaga
    12301 tagtcttgga aattcatatc aaatccgaaa atcagtcagt tagcgcgtgc cgaaaatctt
    12361 gtcgcccgca tcgcccagac cgggaataat atagccgttt tcgttcaaac ggctatccag
    12421 cgcggcggta taaatcgtaa catcgggatg cgcatcatta acagccttca cgccctcagg
    12481 ggcggcaacc aagactaagg ctttgatgtt gcggcaacct ttttctttca acaggtcgat
    12541 ggtggccacc atcgaaccgc ctgtcgccag cataggatcg ataatcaaag ccggacgctc
    12601 gtccatactg tccacaaatt tctcaaaata ggaaataggc ttcagcgttt cctcgtcgcg
    12661 ctgcaaaccg accacgctga ttttggcagt cggaatcagg tcgagcacgc cgtcgagcat
    12721 acccaaaccg gcgcgcagga tggggacgac ggtcagggtt ttgcctttga tgcggtcgcc
    12781 ttcaatctgt ccgcaccatc cgtcgataag gtatttttca atttcaaaat cacggcttgc
    12841 ctcgtatgcc atcaggcgcg ccaactcggt ggtcagcgta cggaatttgt aggtgctgca
    12901 atccgcctcc ctcatcaggg tcagtttgtg gcggacgagc ggatggttga taacgttaac
    12961 gttcatcgcg gtgttccagt gtgattttga gacgcgtcat tatacttttt tttaatttgc
    13021 cggaaaagaa aatgccgtct gaaacacgtt cagacggcat tgccgaatcc atcaggcttt
    13081 cagcagctct tgcagctcgc ctgcctcata catttccatc aggatgtccg aaccgccgac
    13141 aaactcgccg ttcacataaa gttgggggat ggtcggccag tcgctgtatt ccttaatgcc
    13201 ttggcgtact tcgggatttt ccaatacgtt gacagcaacg taatcggtgc agcctgccgc
    13261 gttcaggatt tgcacggcgc gggaagagaa accgcattgc ggaaactgct tcgtaccttt
    13321 cataaacaat acgacgcggt gtgtcgttac tacttcttta atttggtcgt ggatggaagc
    13381 catcgcttat tccttttgat aaagttgaca aaatttgtca ggtagggcgc attatacgca
    13441 aaagcggaaa cttcccacaa tatttgccgt atatcgcgca gtatcaatgt tgcgaacggc
    13501 aatattccca ctcctcgaca atcctgctgt ccggcaaatg gcacagggca tatccgccgc
    13561 cgctttcgtc tttttttact tcccgtctgc cgcctttggc aatgcagaat ttggaagcgg
    13621 aatttgccat gcctgtccga aacgtgttgg caggcagccg gcagcagcgc ggacatgcat
    13681 acggatgaat tgccttcatg gttttcctta tcgtcggcac agggtcagac gacccgcata
    13741 tcgggtactt ttttaaacag ccggtaaaag ttttccgtcg tatatgccgc aacctgttcc
    13801 aatgtttgat tccgcaattt ggcaatatgt tcggcagtat gacgcacaaa agccggttcg
    13861 ttctgcctgc ctcgtttggg aacgggggca aggaacggcg catcggtttc cactaaaatg
    13921 cggtcgtccg gaacgtattt cgccgcctcc tgaaccaagg gggcgttttt aaaggtaacg
    13981 attcccgaga aagaaatata aagccccaaa tccatggcag cccgggcaaa accgacatcc
    14041 tcggaaaaac agtgaatcac acccgaattg gttttacatt cttttaaaat cgccaaggtg
    14101 tccgccgccg catcgcgcgt atggacgata acgggcagtc cggtttgatt ggctgcttcg
    14161 atgtggtctg caaagcgttt gtgctgccaa gacaaatcgc ctttgcacca gtaataatcc
    14221 aaacccgtct cgccgatgcc gaccactttc ggatgggcgg cggcttcgac catttccgca
    14281 atggaaaatt cttcggcttc cttgctgtcg ggatgtacgc ctatggtgca atagatgtgt
    14341 tcgtgcgctt cggcgacggc aaagacttcg gagaagcttt ccctactgac gctgatggcg
    14401 agtgcctgcc ccacgccgtt tgcttccatg ttggacaaaa cttcgggcag gcgttctttc
    14461 aaaccttcaa aattgaggtg gcagtgcgaa tcgatgatat gcatagcgtt acatggtaaa
    14521 cgtaggtctg ctgccgctga ttgtgctgcc cagttcgact tcgatctggt ctttgacttt
    14581 caggcagttt tcgtgttttg tgaatgccag ccccacccct ttgggtttgg aggaagtact
    14641 cgcaggatta atccaggcga ccttggtcgg caggaacagt ttggggaagt tgaggatttc
    14701 tacggcaagc agaatatcgt ccccgatgga aaatacgtcg tcggtctgca caaacagacc
    14761 gccgtgttcc aaaaacggca tataggagct gtacagcaga ttcatgtctt tcagctgcaa
    14821 cgacatcatt tttgccggaa tattttgtcc gtctgacata attcacctgt tctctttctt
    14881 caattccaaa tagttgatga gcagatgctc gatctgcatt ttgacattta aagtatgaaa
    14941 cccgtaaggg gcaagctgtt tgagcatatc ctccgccgca aatacattgc gcggacggaa
    15001 acccgatgcc gtctgaagca gcctgtcttc ataagcggga taatagacgg gtttcatgtg
    15061 ttgcaggcac aagcctaaat cgaccagcca tttctgcatc cacccgacaa atacggcgag
    15121 cggaagtttt tccttatcga aaagcgcggc gtaatccaaa atcttcaaca atctcggctc
    15181 tgccaatatc tccagcagct ttgccctcaa ttcccgaagc tcgccttcct cctgaaacag
    15241 cggcgcaccc gaatggaaag ccagacgttc ctcaggctcc gccacacccc tgtcgcgcag
    15301 atacgccaat gcttccccat gcgaaggagc gggcaaaacc ttcttccggc agcggctttt
    15361 aatggtcggt aaaaccttgt ccgccgcgtg gcttaccagc aaaaagacca cttgtggcgg
    15421 cggctcttcc aacactttca acaaactgtt ggcggcttgg acattcatac tttccgcagg
    15481 atgaatcagg atcacgcgca aaccgccccg taccgaagtc aggtacacat tatcgatgat
    15541 ttccctgacg gcatcgattt tgatccgcaa cagtttgcgt ccgttttcgg gttcgtccgc
    15601 cagaggcgtg atttcgtaaa aatcgggatg gcttccccgt ccaaacagat ggcaggacat
    15661 acattcgcca cagggtttgc agcccggtgc aggggtttcg cacaacagcg ccttcgccgc
    15721 aaagcgggca aacgcagtct tccccgtccc ctttttgcca acaaacagcc acgcattagg
    15781 acggctcgtc caatgctcgg caatctgccg ccattgttcc tgatgccacg gataaatcat
    15841 gtcaaactgc ccgtccggaa atccataccg ccatcagagc agttccaaat cggggtcgga
    15901 actttgtgcc ctgcggctgc gcaactgtac cgccaaacgc aaatcatgtg cggaatcggc
    15961 atttttcaag gcatcctgca aggaaatctc gcctttttca tacaattggt aaaggtgttg
    16021 gtcgaaggtc tgcataccca gggtagtgga ttttttcatc acttctttga tttcatggat
    16081 gttgccgttg tgaatcaact ccgaaatcag gggcgaattg agcagcacct cgactgccgc
    16141 caccctgccc ttgccgccgt ctcgcggaac gaggcgttgc gaaataaacg cctgaaggtt
    16201 gagcgacaaa tccgtcagca attgttcgcg ccgctcctcg gggaagaagt tgatgatgcg
    16261 gtcgagcgcc tgattggtgc tgttggcgtg cagcgtcgcc atacacaaat gccccgtttc
    16321 ggcaaaggcg atggcgtagt ccattgtttc acggtcgcgg atttcgccga taaggatcac
    16381 atccggcgcc tgacgcagcg tatttttcaa cgccgccatc cagttttccg tgtccacgcc
    16441 gacctcgcgc tgggtaatga tgcagttttt gtgttcgtgg acaaactcga tcggatcttc
    16501 gatggtgatg atgtgtccga acgaattttc attgcggtag tcgataagcg aggcgagcga
    16561 agtcgatttg cccgagccgg tgccgccgac aaaaataacc agcccgcgtt ttttcagcgc
    16621 aacatccttc aaggccggcg gcaggttcag gctttcaaac ttgggaatct tgctggtaat
    16681 cgcgcggaat accaacgccg tcgcaccgcg ctgtatcatc gcattgacgc ggaagcggct
    16741 ggtgtccggc aggctgatgg cgaagttgca ctcgttggtc gatgaaaatt cttccgcctg
    16801 cttcgcactc ataatcgaaa aggcgatttc catacatttt tccgccgtca gcggttcgtc
    16861 cgtgatgcgg gtgattttgc cgtccagctt catagcgggc gggaaatggg tcgtcacaaa
    16921 caggtcggaa cctttgtttt tgttcatatg gcgcagccat gcgaacagtt cttcctttgc
    16981 agtcatattt tcatcggtaa acatcctcgt cccccaatct tcaaacaatc tgtaccttcc
    17041 cattctacca aaaatccgcc gtgctgtctg aaaccgtttc agacggcacg gacaaaccgc
    17101 ctccactcaa tcctgccgtg ttagcgtgta taattcggcg ttctatccga aattcacagc
    17161 aggttagcaa tatgtctttg aaaacagtag ccgttatcgg cgcgatggaa caggaaatcg
    17221 agcttttgcg cgagatgatg gaaaatgtca aagccgtctc tttcggcaga ttttccgcct
    17281 atgaaggcga attggcggga aaacgcatgg tgcttgcatt gagcggcatc ggcaaggtca
    17341 acgcggcggt tgcaacggct tggcttatcc gtcaattcgc accggactgc gtcatcaaca
    17401 ccggcagcgc aggcggtttg ggcaagggtt tgaaagtcgg cgacgtggtg attggcacgg
    17461 aaaccgagca ccacgatgtc gatgtaaccg cattcggcta tgcccggggg caagtgccgc
    17521 aactgccggc aaggttcgct tcagacggca tattgattga aacggcaaaa cgggcggcgc
    17581 ggacgtttga aggcgcggag gtagaacaag gcctgattgt cagcggcgac cgctttgtcc
    17641 acagcagcga aggcgtggcg gaaatccgca agcacttccc cgaagtcaaa gcagtggaaa
    17701 tggaagcggc ggcgattgcc caaacctgtc atcagttgga aacgccgttc gtcatcatcc
    17761 gcgctgtttc cgattcggcg gacgaaaagg cagacatcag ctttgaagag tttttgaaaa
    17821 cggcggcggc aagttctgca aaaatggtgg cagaaatcgt caaatcttta taaaaccttt
    17881 atcaaatcat gccaaaacag cggttttccg ttagaatatc gggctatttc cacgttgcgc
    17941 ccgatattcg gacgtaccgc tgtttgttca gacggcattt ctaaaaccta ccgttcagca
    18001 ttaaaaacaa ctcaatgaaa aatatccgaa atttctccat catcgcccac atcgaccacg
    18061 gcaaatcgac gcttgccgac cgcttcatcc aatattgcgg cggtttggat ttgcgcgaaa
    18121 tgagtacgca ggtgctcgat tccatggaca tcgaaaaaga gcgcggcatc accatcaaag
    18181 cgcaaaccgc cgcactcaac tacaaagcac gcgacgggca ggtgtatcag cttaatttga
    18241 ttgacacgcc gggacacgtc gacttctctt acgaagtttc ccgttcgctg tcggcttgcg
    18301 aaggcgcgct tttggtcgtt gacgcgtcgc agggcgtgga agcgcaaacc gtggcgaact
    18361 gctataccgc gattgatttg ggcgtggaag tcgtgcccgt tttgaacaaa atcgacctgc
    18421 ccgccgccga ccccgagcgt gtggaacagg aaatcgaaga catcatcggc atcgatgccg
    18481 tcggcgcggt gcagtgttca gccaaaagcg gcatcggcgt ggaagacgtt ttggaagaaa
    18541 tcgttgccaa aattcctgcg ccgaccggcg acgaaaacgc gccgctgcaa gccgttatcg
    18601 tcgattcgtg gtttgacaat tacgtcggcg tggtcatgct gattcgagtg aaaaacggca
    18661 ccatcaaact gaaagacaaa gtgcgcttta tgagcaccaa ggcggaaacg caggtcgagc
    18721 agctgggcgt attcacaccg aaatcggttc aaaaacaaga actaaaagcc ggcgaagtgg
    18781 gttttttgat taccggcgtg aaagaattgg gacaggcgaa agtcggcgat acggttactt
    18841 tggttgccaa ccctgccact gagccgctgc ccggtttcca agaagtacaa agccaagtat
    18901 ttgcgggtct ctatcctgtg gaaagccacg attacgaagc cttgcgcgac gctttggaaa
    18961 aattgcagct gaacgatgct tcgttgaaat tcgagcctga agtttctcaa gcattgggct
    19021 tcggcttccg ctgcggcttc ttaggcctgc tgcatttaga aatcgtgcag gaacgcttgg
    19081 aacgcgagtt cgacatggat ttgattacca ccgcgccgac ggtggtttac gaagtcgtgt
    19141 tgaaaagcgg cgaaaaaatc gaagttgaaa acccgtccaa actgcccgac atcggcagca
    19201 tcgaaaccat tctcgagccg attattaccg cgaccatcct tgtgccgcag gaatacgtcg
    19261 gcaacgtcat gactttgtgt aaccaaaagc gcggcgtgca ggtcaatatg cagtatatgg
    19321 gccgccaagt gatgctgact tacgacttgc cgatgaacga agtcgtaatg gactttttcg
    19381 acaaactcaa atccacttcg cgcggttatg cttcgttgga ctaccatttc aaagaattcc
    19441 aaccgtctga tttgattaag ctcgacatta tggttaacgg cgaaaaagtc gatgccttaa
    19501 gcctgattgt acaccgtcaa agcgcggttc acaaaggccg cgagctggca tcgaaaatgc
    19561 gcgaactgat tccgcgccaa atgttcgaca tcgccgtcca agccgccatc ggcagccgga
    19621 ttatcgcccg agaaaacgtt aaagccctgc gtaaaaacgt tttggcgaaa tgttacggcg
    19681 gcgatattac gcgtaagaaa aaactgcttg aaaaacaaaa agcaggcaaa cgccgtatga
    19741 aacaagtggg caacgtggaa attccgcaaa gcgcgttctt ggcgattttg caggtgagcg
    19801 acaaataata tttcggcaca tagggcgggc gcgtcgggaa cacctctcaa accgccctga
    19861 tttcagcggc acgcgcccgc aaacaaattt tgcaatttgc cgccttaaaa cggttgcacg
    19921 gccgggcggg tttttaaccg ttaaggaaca acaatgaaca caatgctaat gtcgggcgcg
    19981 gctgccgcgc tgcttgccgg catcatcctt tatttcaaaa gcgacaagaa acggcaggaa
    20041 aacggtgaat ggagttccgg cctcgaatac gcctatatcc tgacggcggt cggtgtgttt
    20101 gccgctttgt ccctgtttat gagctttacc gccgttttcc tgattttcgt tgtattgtgc
    20161 ggtacggctt ggggggtata taaataccgc ctgaagactc atcccgaaat ctcggaaagc
    20221 agccacttcg gcgattattt cggcagtttc ttccctaccg ttttggtatt gttcctcatc
    20281 cggtcgttta ttgccgaacc gttccaaatc ccgtccagct cgatgcgccc gggattaatc
    20341 aagggcgatt tcattttggt cggcaaattt tcctacggcc tgcgcgtacc cgttttaaac
    20401 aatgtattca tccccacggg caaaatcgaa cggggcgatg tcgttgtttt taactatcct
    20461 ttgcagccgg agatgaccta catcaagcgt attgtcggca tcccgggcga cattgtcgaa
    20521 tatcgagata aagttttgac ggtaaacgga aaaccggctt ccgacattcc tgacggcaca
    20581 taccgttatc ctgacgatac cgacccatcc gaaatccaca acacggatat gttccgcagc
    20641 ggtctggacg gcaaatcctt caatattctg aaaaaagaag gacagcctgc cgtttccctg
    20701 cccgtattgg gcaaatatac ctccgatatt atgtctgaaa acggatattc catagatcaa
    20761 agcggtctga accattgcca atatgccgac gacggcagcg gtttcgtgtg caaagttccc
    20821 gaaggacgct atttcgctat gggcgacaac cgcgacaaca gtggcgattc gcgctactgg
    20881 ggctttgtgg atgacaagct ggttgtcggc aaggcaatgt tcattttgat gaacttcggc
    20941 gatttcggca gggccggcac ggcaatccgt taagccggaa aattacgccg gaacatcaaa
    21001 aatgccgtcc gaaccctgtt tcagacggca tttttatcat caaactcctt gtttccctac
    21061 cggcagcctc ggggataata tagtggatta aatctaaacc agtacagcct ggcctcgcct
    21121 tgccgtacta tttgtactgt ccgcggcttc gtcgccttgg cctgatttga atttaatcca
    21181 ctatatgcac cgccttgccg gcattatcga tatgggcggc acatatggat tgcctggaaa
    21241 aatgatgcgt tcgggcggcg tatgatttgc ctgtgtttaa atcatcggtt gattttatcc
    21301 gtcggcaagt gaaaagccgg ttatttccgt ttgtgtggaa acccatccca ttgaataaag
    21361 ggcatttcgt ccgactgacg gtgtgggcgg catatggaaa tacggacatt attttagttg
    21421 cattgccatt cctgcggagg cggctttttt gattctgccc gccccgatgc cgtctgaaag
    21481 ggcttcagac ggcattttcc gctccgcgcc gttgtcggca tgtttggtct tgccggtttt
    21541 gactgcctgc cagtccaatc gtacgcaggt ttgccacggc agaaaatctg atgccgaccg
    21601 gagtcaaagc tcggacggct gtttcagcga accgaggtaa atcgccgaaa gcgtcagcac
    21661 cacccctgcc cactgcacgc cttcaatcgt tttgcccaat ccgaaataat cgatgaacag
    21721 ggcggcgacc ggttcggaca aaagcaacag ccccgtcagc gacagggaaa gcagcggaat
    21781 cgcataggca accatcgccc acgcaaaaca ctgcatcacc acgccccgta cacaagcacc
    21841 aaacccgaat ctttccaagt ggtcggataa agtgcgccgc cgtccatcag caatgccgga
    21901 acaatcggcg atgccgcgcc gcccaaactc aaaatcatca ttgaagggaa aagcgccacc
    21961 tgttcgactt catgagtctt gcggacgaac accatagata gggcaagcat cagcccggac
    22021 accaaaccgc tggcgaatcc ccaaaccgca ttaccgttgt agccgaattc cgcaccggca
    22081 atcatcgcca cgcccacaac cgccgacatc aagcttgccg cctgcagcct gcccaaacgc
    22141 tcgccgaaaa agaaaacacc gattgccgac aagaaaaaga tttgcaggct gttgagcagg
    22201 gtggaaatac ccggcccgac cgcgtgtatg ctttcgtgcc aaagtgccaa atcgaaagcg
    22261 agaaacacgc ccgccgtcag ggcatatcgg acggtttttc tgttttttgg gaatttttgt
    22321 ccgaaaaacc gtgccaaaaa ccaaaatacc aataccgaaa tcagcaaccg ccaaaatgcg
    22381 attgcatacg ggccgacggg gacggatctg acaatcaggc tgcccaatcc gaacaccgcg
    22441 caacccagca ccagcaatgg tgcggcatat ttttttccgt taccgaccac aatatcccct
    22501 cccttcccca tatggctgcc gcgccgccgg caaaagtgcc gccattccga aaatatgccg
    22561 tctgaagccc aaagccccgg ctgcgcccgg acaaacggct gactttacgg gcaggccggc
    22621 tattttccgt tggggcaaaa tccgtcctgc cggataaaac ccgaataaaa aatatttccc
    22681 tcaagcaatg cggcggcgcc aacacctgat taaaacacaa ccggcgtttt cggtatatat
    22741 gcaaaaacgc attttccggc acggcgttac accgataccg aaaaatgcgt ctgaatgttt
    22801 cgacaatccg attaaagcat tatgccaaat ctgcaaaaag tggcgtagag agatagcgtt
    22861 cgccataaga aggcagcagc acgactatca gcttgccttc gttttcaggc tgtttggcaa
    22921 gctgcaacgc gctccaaacc gccgcaccgg aagaaatgcc cgccaaaatg ccttcttttt
    22981 ccgccattgc acgggcggtt tcaaaagccg cttcgttcgg cactttggta atgctgtcgt
    23041 agatttcggt attcaaaacg gtcggaataa aacctgcgcc gataccttga atcgggtgcg
    23101 ggcctttttc gccgccgctc aatacggggg aagcctcagg ctcgacggca accactttaa
    23161 cttcgggttt gtattttttc aatacttcgc ccacgccggt aatcgtaccg cccgtgccga
    23221 cgccggcaac gaagacatcg actttaccgt cggtatcgtt ccaaatttcc tcggcggttg
    23281 ttttgcggtg gacttcggga tttgcctcat tgtcgaactg gcgcggcata aaataagtgt
    23341 ctggatgagc gtccaccaag gattgcgctt tggcaatcgc gcccgccata ccttcggcgg
    23401 cgggggttag aatcagttcc gcgccaaacg tgcgcaacag cattttgcgc tctttgctca
    23461 tgctttccgg catggtaatc gccagtttgt agccgcgtgc ggcacatacc attgccaaac
    23521 cgatacccgt attgccgctg gttgcttcga caatgacggt gtttttgttg atttttcccg
    23581 ctttttcggc ggcctcgatc attgcttcgg caatgcggtc tttgacgctg ctgcccggat
    23641 taaaaaattc cagtttcacg gcaacctctg ccttcaaacc tttggtcaaa cggttcagtt
    23701 tgaccaaagg cgtgttgccg atcaattcgg tgatgctgtt tgcaattttc atcttctgct
    23761 cctgaataat aaggtttcga cagtaaggcg caatccccat cggtatcctg tccgattgca
    23821 ggggatttgc gccgaaccat aaacaaattt cggatatggc tttatcctaa acctatgccg
    23881 cctaaaaaac caatacccaa aatttatttc tttataactt tttgttccga tacagacggc
    23941 atcacaatca tctgccgatt tcggcaagca gcatcgccgt taccgcaacc tgcatcaaca
    24001 tcgccgccaa cacgccgagt cgcagctgcg aaccgccgaa cgttgacggc ggctgccggt
    24061 ctataccgca gcggtggaca taggcaaccg ccatcaatac aaactgcaac acgaaccaat
    24121 aaaccggctt cagcgcataa agtttgagca atgccgcaaa cacaaacact tgaaccgttc
    24181 ccgaaaagca ataatgcacc aacgcacata ttccgacaaa agcaagcgtc atcgatacat
    24241 tactgacggc aaaaagcccg agcagcggat gttcggggtc tgcctgccat ccgtttccat
    24301 ctgttttccg gttccaatac ccgatgaaaa aaaatatgct gcccaaagtc aggtaaaaat
    24361 gggggatgaa caaaggcgcg gcgcgggtca ttccccacat ccccggcatc agctttaccc
    24421 ctaaaaccga gatgccgagc cacaacgcaa tactcgccca aaaccattgc aacattcctg
    24481 cgcggaatat cagaaacaga aaaaccgccg catacagtaa ggcagacaaa aaatccaaat
    24541 gcagcaaaat catgaatacg ccaactcgcc ttcaaacacg gtttccgcag ggccggtcat
    24601 catcacatcg ccgccgcagg cccattcgat atataaagtc ccgcccggca aaaccacctc
    24661 taccgttttc ccttcatcca acagccccag acggataccc gccaccacag ccgcacacgc
    24721 gcccgtaccg cacgcttggg tttcgcccac gccgcgctcg aacacgcgca aacggattgc
    24781 cgtccgtccg acaacctgca taaagccgac gttgacgcgt tcgggaaact gcctgtgcgg
    24841 ttcgatacgc gaaccggttt cgcgcaccgg cgcgcattcc acgtcatcga ctacaatcac
    24901 agcatgagga ttgcccatat tgacgcagct gacaggctga atgccggatt cgagatgcac
    24961 cccgtaaata caggcatcat ccccctcgcc cgattcgggg acaaacggta tttcagacgg
    25021 cataaacttc ggtttgccca tattgaccgt aaccataccg ttatcggaca atttcggaaa
    25081 aataatgccc ttggccgttt caacacaaat ttctttctta tcggtcaaac ccttgtctgc
    25141 aacaaaacgg gcaaaacaac gcgctccgtt gccgcattgc ccgacctcgc taccgtcggc
    25201 attgaaaata cggtaacgga aatccacgcc ttcggtttcc gaacacccga ccaccaaaag
    25261 ctggtcgaag cccacgcccc ggaagcggtc cgcccatgcc gcaatcggca cgtcctcggg
    25321 ggtaaaatcc tgactgaccg cgtcaatcac cataaaatcg ttgcccaaac cgtgcatttt
    25381 ggtaaatttc aaagttttca tattgttccg aaatcccgta ttgtctgttg cttccgaatc
    25441 cgaaatcccc gagcccgaag ggaaatgctt ccatctcccg ccgcctgagg attaaaatga
    25501 gccattatac tgtttgttac cccttacaag aaacacaatg tcccgcctgt tcctgctgct
    25561 tttcccttgg gcaacccttg ccgcctgcac ccgaaacata ccggcaagcc ccgccgtttc
    25621 aaattgcgcg catcttcaca accgcaccgt cctgtatttt gacgcacacg gcaaacccgc
    25681 cgacagcaca gcaaagggcg gattttacag gaccgtcgaa attcatgaca caaaccgcat
    25741 ccttattcaa gactttaaca gcacggggaa tgccctcgtc aaaccctatt ggtcggactg
    25801 ccggaaattg tccgttttcc ccgacaaaca tctgaccatg agccatccat gaaccacgac
    25861 atcactttcc tcaccctgtt cctgctcggt ttcttcggcg gaactcactg catcggtatg
    25921 tgcggcggat taagcagcgc gtttgcgctc caactccccc cgcatatcaa ccgcttttgg
    25981 ctgattctgc tgcttaacac aggacggata agcagctata cggcaatcgg cctgatgctc
    26041 ggattaatcg gacaactcgg catttcactc gaccaaaccc gcgtcctgca aaatatttta
    26101 tacacagcct ccaacctcct gctgctcttt ttaggcttat acttgagcgg tatttcttcc
    26161 ttggcggcaa aaatcgagaa aatcggcaaa ccgatatggc gcaacctgaa cccgatactc
    26221 aaccggctgc tgcccataaa atccataccc gcctgccttg ctgtcggaat attatggggc
    26281 tggctgccgt gcggactggt ttacagcgca tcactttacg cgctgggaag cggtagtgcg
    26341 acaaccggcg gactgtatat gcttgccttt gcactgggta cgctgcccaa tcttttggca
    26401 atcggcattt tttccctgca actgaaaaaa atcatgcaaa accgatatat ccgcctgtgt
    26461 acaggattat ccgtatcatt atgggcatta tggaagcttg ccgtcctgtg gctgtaagcc
    26521 ccattcattc ccgatacccg taaaaaatac cgtctgaaac ctgttcagac ggtattttca
    26581 ccctaagcgt tactcggcgg cattttcttc acggacaggc gcatccagca gggctttgat
    26641 ggacagacgg acacgacctc tgtcatccac ttccaatgct ttcacgttca ccacctgacc
    26701 gacttgcagg taatcgccga cattgcgtac gcgctcgtgt gcgatttggc tgatgtgtac
    26761 caaaccgtct ttgcccggca tcacgctgac aatcgcgccg acgttgttgt cgaggatttt
    26821 aaccacagta ccttcgtaca ctttgcccac ttccacttcg gcagtgattt cttcgatgcg
    26881 ttttttcgct gcatcgcccg cttcttgagt agtcgcagcg atagtgatgg taccgtcttc
    26941 agcgatattg atttcagtac cggtttcagc ggtaatcgaa cgaatggttt cgccgccttt
    27001 accaatcaca tcacggattt tgtcttggct gattttcatg gtgaacaaac gcggcgcgtg
    27061 tgcggacagc tcttgcggac ccgcaacggc agctttcatc tgatccaaga tgtgcaaacg
    27121 cgcttctttg gcctgtgcca aagcaatttg cataatctct ttggtaatgc cttggatttt
    27181 gatgtccatt tgcagcgcgg taacgccttc ggtcgtaccg gccactttaa agtccatatc
    27241 gcccaagtgg tcttcgtcgc ccaaaatgtc ggtcaggacg gcaaacttat tgccttccaa
    27301 aatcagaccc atcgcgatac cggcaacgtg tgctttcaaa ggcacgccgg cagacagcag
    27361 gctcaaacag ccgccgcaga cagaagccat agaggaagag ccgttggatt cggtaatttc
    27421 ggagaccacg cgcatggtgt agctgaaatc ttcaggtttc ggcaatacgg ccaacaacgc
    27481 acgtttagct aaacggccgt gaccgatttc acggcgtttc ggcgcgccca tgcggcccac
    27541 ttcgccggta gagtacggcg gaaagttgta gtgcagcata aagcggtcgg tatattcgcc
    27601 ggacaacgcg tcgatgattt gttcgtcgcg cgaagtaccc aaagtcgcta cggcaagggc
    27661 ttgggtttcg ccacgggtaa acagtgcgga accgtgcgtg cgcggcaata cgccggtttg
    27721 gatgttcagc ggacggacgg tgcgggtgtc gcggccgtcg atgcgcggtt gtccgtccaa
    27781 aatttggctg cggacgacat cggcttccaa gcgtttgaaa atacctttga tttcgttggc
    27841 tgccagcgtg tcggtttctt cggtaatcaa ggcttctttt accgcattcc aagcctcgtc
    27901 caatttggcg gaacgcgctt gtttttgacg gattttgaac gcttctttaa tggtttcgcc
    27961 ggcaatcccg cgtactttgg caaccagttc ttcattggtt tcaggtgctt tccaatccca
    28021 aacttccgga ttcacttcgt cggcaaattc attgattgca ttgatggcaa cctgcatttg
    28081 atcgtggccg taaaccaccg cgcccaacat cacgtcttcg ggcaggattt tggcttcgga
    28141 ttccaccatc aacacggctt tggaagtacc ggcgaccacc aagtccaatt gtgatttcgc
    28201 caactcggct ttagtcgggt tcaaaacgta cacgccgttt atataaccca cgcgcgccgc
    28261 gccaatcggg ccggcgaacg gtacgccgct caacaccagc gcggcggacg cgcccaacat
    28321 tgcaggaatg tcggaatcga tttcgggatc gacggacaca accatcgcta cgatttggat
    28381 gtcgtggtag aaaccttcgg ggaacagcgg acgaatcggg cggtcgatca gacggctggt
    28441 caggatttct ttttcgcttt gtttgccttc gcgtttgaag aagccgccgg ggattttgcc
    28501 tgcggcataa gtgcgttcca aataatcgac ggccaacggg aagaagtcct gaccttcttt
    28561 gacctctttg ttggtggtaa cggcaacaaa aacaacagtg tcgcccatcg atactttaac
    28621 ggcagcggca gcttggcggg caatttcgcc ggtttccaaa gtaacggttt gattaccgta
    28681 ttggaaggtc ttaacgtatt tattgaacat cattgttcct ttcaaaatac cgcactgcta
    28741 aaacactaat aatgcacact aaaatccgaa tgtgcatagt tagggtttca gaccgtgcgg
    28801 caggttgtaa acaagctttc agacggcatt tcggacgctg aaagcaattg cggattataa
    28861 aggcaaccat ccttaaaatc cagtattgat acaataaaaa ggccgtctga aacatatcct
    28921 tttcagacgg ccttcagtct tcaaaatcaa tcttgtgtca gtaaagtcaa tgcttcctga
    28981 tatttctcga ccgttttccg aattacatcg gcaggcactt taggcgcagg ggcttttttg
    29041 ttccaaccgc tttgttccag ccagtcgcgg acgaattgtt tgtcaaaaga cggcggattg
    29101 atgccgactt catattgatc cgccggccag aagcggctcg aatcgggagt caatacctca
    29161 tccatcagcg tcagcgtacc ttcttcatcc aaaccgaatt caaatttggt atcgcaaatg
    29221 atgatgccgc gcgatttggc atattccgcc gcttcggtgt aaagccgaac cgctttgtcg
    29281 cgcacttctt ccgccaattc tttaccgata atgcgtccgc attcttcaaa gctgatgttt
    29341 tcatcgtgat cgccgactgc ggctttagtt gagggcgtaa aaatcacttc gggtagttgt
    29401 tgcgcttccc gcataccttc gggcagccga ataccgcaaa ccgagccggt tttttgataa
    29461 tctttccaac cgctgcctgc cagataaccg cgcacaatgg cctccacttt caccggagtg
    29521 agttttttag ctacaacggc gcgtttttcc aaagctttgg cttcgttttc aggcaaaaca
    29581 tcgtacacgg tatcgccggt gaaatggttg ggcataatat gcgccagttt tttaaaccaa
    29641 aaattggaaa tctgcgtcag gatttcgccc ttacccggaa ccggggcgtc caaaatcaca
    29701 tcaaacgcgg acaggcggtc ggaagcgacc atcagcatac gtttatcgtc gatttcatat
    29761 aaatcgcgca cttttccgga ataaatcttt accaaaccaa tctcactcat ttcgcacccc
    29821 cctgaaaata tcttgaaaat accgacccga caggtttgaa tcgcaaaccg atattctagc
    29881 cgaagtcggc gcaaaacaat acccatggca caaaaagcca acccgtcaac cgtcggcaaa
    29941 gttttggcac tataataccg acagcaagtc ctacaataca ctttcaccaa aggaaatacc
    30001 tcatgagaat cctattgaca ggctcgaaaa gccaactggc acactgcctg cgcgaccgtc
    30061 ttcctgaaga ctgggaaacc attgcgacgg attccgcatc cctagacatt accgatgccg
    30121 atgccgtctg caacatggtc aaaagtttcc aacccgacgc tattgtcaac acggctgcct
    30181 atactgccgt cgacaaagcg gaaggcgatg cggcagcggc atttgccgtc aatgcttccg
    30241 ccgtttacaa ccttgccttg gcagcacatc gcgcccatgc ccgattcatc cacatctcaa
    30301 ccgactatgt ctttgacgga aaagggaaaa taccccatca ggaaagcgac tttaccaacc
    30361 cttccaatgt atacggacaa tccaaaaccg ccggcgaact gctcgcactg tctgccaatc
    30421 ccgacagcct tatcctgcgg acttcttggc tgtttagcga atacggggac aactttgtcc
    30481 gcacgatgct gaaccttgcg cgggaacgtt ccccgctgtc cgccgtccac aaccaaatcg
    30541 gctgcccgac ctatgccggc gacttgtccg ccgccatcat ccgcctgttg cagcagtcca
    30601 atcccgttcg cggcatttac cactacgccg gcggcaaatc cgtatcctgg tacgaatttg
    30661 cccggcacat ctttcagacg gcattgcaac aggatgcctc ttttcccgtt cccgaactga
    30721 aagccgtttc ggatgaaggc tctgcggctg ccgcccccag gcccgcatac agcattttgg
    30781 actgccgcaa aatcgaaaac gacttcggca tcaaaccgtc agactggcaa aaagcccttg
    30841 cacaggtcgt ttccaagctg ctctgatgcc gcccgcccct ccgtttcctc cgccaagcac
    30901 cgccttggcg gttttcttat aaaatctact gattacacaa acacatatca tctttacaca
    30961 atcatgcttc catcaacagc aaaacatgat atgattgcca acaatgacat ctcacaataa
    31021 attttctaat ttttattgaa aaaatcaata aattaagaat tctcccaacc gacaaataaa
    31081 ataaagaaag ggtcaatatg caacaccgta gaagattggc aatttaccag gcatccaaac
    31141 gtgcttcctt taccggcagg tcatcagccc cacaaaaacg taaagaacgt tgatttgaaa
    31201 aaatgccgtc tgaagtcctg cttcagacgg cattttttac cgttcgagaa actgtttcaa
    31261 cctgtcctca tccaaaaacc agtggcaaat ttcctcatct cccgccaaca aaacgggaat
    31321 cagctcattg tatttttctt ccaaaacagg attttcatcc acatcgacca cttccagccc
    31381 gaacccgtat tcatcctgaa aaggtttgag tgcatcgcgc attttgtggc acaagctgca
    31441 atattcacga aacatcaagg tcaatttcat ccgcgtttcc ttatctgtca atttgcacac
    31501 gccaaagcct tagacgcagc agaatcatgg tctatttggg aaaaaacaat gtcttcgagg
    31561 aagatgatac tcaagtcctg ccaaaaacag caaaaatgcc gtctgaacag ttcagacggc
    31621 atttccaaaa ccgttttacg cttgaacgtt gatacccgcc gtaaccgtcg gttgcgcgtt
    31681 gtgcgccgtc agaaacgaag cggcaagctc cccgcagcag ccgcagcagc tcgccacgcc
    31741 caattgcgac tgcaaatcgc ccattgtggt cgcgccggca gcgatggttt ccttgatttg
    31801 atggtcggta acggcattgc agatgcagac aaacattttg tgctccgtgt gttctcaaac
    31861 tatcgtaccg atagcggctt ttattatcgt atgcaaatat aaataaaaac ggttcgcatt
    31921 gcaaggtcgg catacacggt ttgtcttggt aattttttat caagtttgca ttttcgattg
    31981 atttcttatt aaaccgaagc agaagcctca gtttcgggaa tggtgttcct taaccacgct
    32041 atgcagccgc acattggcaa catgggtata aatctgagtc gtgttcaaat cggcgtgtcc
    32101 caacatatcc tgaactacgc gcaaatccaa tccgtgccgc accagatgcg tggcaaaggc
    32161 gtggcgcagg ctgtgcgggc tgatgtgccc gatgcctgcc ctgccggcat attctttgac
    32221 aatcatccac gccaactgcc gggaaatgcc cgtttttttc tgactgacaa acaatgcgtc
    32281 gcaactcctg cctttcagca gaagcgggcg tgcctccgta taatagcgtt ccacccaata
    32341 cgctgactcc tgtcccatcg ggaccatcct ctgcttgtcg cccttgccca gcgtggtaat
    32401 acaacccctg tccaaatcca cattgccgaa attcagcccg accgcctcgc tgacgcgcaa
    32461 gccggtcgcg tacatcaatt cgagcagggc cttatcccgc aaaccgtgcg gcgtgtcggt
    32521 atccggtgcg gcaagcagtc gggaaatctg ctgttctgta atcagtgtcg ggatgtttct
    32581 gtcgattttt ggcggtttca gcaggcgggt gggattgtcc gccctcatcc cttcccgttc
    32641 catccacaca tacaggcgtt tgcacgccga cagtgcgcgt gcctgcgaac tccgttgctc
    32701 cccgtcaaca taaaccgccg ccgccaaatc tgcttcgtcc gcatccttca gcgttctgcc
    32761 gcacaaagac agccggcggg cgatcttttc caaatcccgc cggtagctgt ccaaagtatt
    32821 ccgactgagc cgccgatcca accacaaagt ttccagcagc ctgtcaatca aaccgttttc
    32881 cataacgttc caaaccaatg ccgtctgaat cttcttcaga cggcatggtt cacatcatcg
    32941 ggaaagcgtt tccaacactt cctgtgcatg acccgccact ttgactttcc gccactcccg
    33001 gacaatttcg ccgtccttgt tcagaacgaa cgtactgcgc tcgatgccta acgactcttt
    33061 cccatacagt tttttcaatt tgatgacatc aaacaggcgg cacaccgttt catccttgtc
    33121 gctcaacagc tcgaaccgga aaccctgctt ggcgcaaaaa ttctgatgcg cctttacgcc
    33181 gtcgcgggaa ataccgacca cggtataacc caatgcctca aactgttcca aacgcgcatt
    33241 aaaatccaag ccttccgtcg tacagcccgg cgtactgtct ttcggataaa aatacacgac
    33301 caaaggcaga tgttctgccg aatgaaaatc cgcaccgctg ctcgaaggca gggtaaattc
    33361 atatttcata tccatagtcc tactcccgat attcccatta ttcaaaacgg cacgcagacg
    33421 caccgccgca attgccaaac caaccccgat tctaccgccc caaaggacaa ggattcaacc
    33481 gccggaaaca tccaaaccga cacacgacgg catgaaaaat atccatgtca aaccacaaaa
    33541 tatgttccga tttaaaaaca gaatgttata aaacccaact ccaaaaacac aacaagaccg
    33601 cccgcctacg ggcagtctcc tgtcagacga catactttac agatggctgt tttttcaaca
    33661 aaataacgcc aatactcaaa aatacggaac caaaaatgtc catccgcacc ctgaaacgcc
    33721 tgccctcatc gctgctgctc ggcctctgcc tttccctgcc gtcagcccac ctttttgccg
    33781 acaacgacat tttagggcaa tttttagaac agaacatgct tacctcctcc gatccgatag
    33841 aaatattcgc cgaaagcacg atatatcccg ccgataccca ggccattacg ggcgggctga
    33901 ttctgtcctc ccaatccgca ctggtcgtca acaacaaaac agggcaaata ctgtatcaga
    33961 aaaacgccga caggattatg cccattgcct ccatttccaa gctcatgagc gcgatggtcg
    34021 ttttggatgc aaacttggac atgaacgaaa ccgttaccat cacacccgac gaaatcgacc
    34081 gcctcaaagg gaccggcagc cgtcttgcca taggtacggc acttacacgt aaagaactgc
    34141 tgcaccttag cctgatgagc agcgaaaacc gcgccaccca tgcattgggc agaacctacc
    34201 ccggcggcat gggcgcattt gtcgccgcca tgaaccgcaa agcccaaagc ctgggcatgt
    34261 acggcagccg cttttacgaa ccgaccggac tcaacttcca aaacgtttcc accgccaaag
    34321 acctgagcct catggtcaac gccgccgccc aatatccgca aatccgcacc aactcgactt
    34381 ccaactacgc ctcggtacaa accaaaaacg ggcagcaaaa ctataaaaac tccaatgccc
    34441 tggtcaggga aggcatgtgg aacatcgaat tgcagaaaac cggctacatc cgcgaggcag
    34501 gcaggtctat ggttgtcaaa gccaacattc aaaaccaacc cgttaccatc gtattgctga
    34561 actcgcccac atccgccaca cgcgtcaacg acgcccgcaa aatcgaatcg tggatgctgc
    34621 agcaacgctc ctgacataca aatgcccggc ggaaaaccgc ccaaacacaa aaaaattggt
    34681 aatttttcct attccaggct acaattcgac acgcacaggg catctgcccg atttcacaat
    34741 cataatagcg gagttaaaaa atatgacagc taaaggacaa atgttgcaag atcccttcct
    34801 gaacgcattg cgtaaagagc atgttccggt ttcgatttac ttagttaacg gtatcaaatt
    34861 gcaaggtcag gtcgaatctt tcgaccaata cgtcgttctt ctgagaaaca cttccgtaac
    34921 ccaaatggtt tacaaacacg ccatttccac catcgtaccg gctcgctccg tcaacctcca
    34981 acatgaaaac aaaccccaag ccgcaccggc ttcgaccctt gtccaagtgg aaaccgtcca
    35041 gcagcctgcc gaataatccg cacgaagcat gacgtgtcat atctttcaat accttaccgg
    35101 acaacggtaa ggtattttta ttttcaggcg gcatttaaaa atattattga aaaacatcct
    35161 tccattcgcc cattgccttt tgcgggaggc acttcccgaa ggcggcaatg cgctggacgg
    35221 caccgccggc aacggacacg acaccctttt cctcgcacaa accgcaggca acagggggaa
    35281 agtgtgggca ttcgacatcc agccgcaagc cctgaacaac acccgatgcc gtctgcagga
    35341 agcaggttac agcaatgtac ggctcatctt ggacggacat gaaaacctga agcaatatat
    35401 tccggagccg ctggatgcag ccgttttcaa tttcggctgg ttgcccggag gcgacaagaa
    35461 tctgaccacc gacaaggaaa ccagcattcc cgccctttga gcaaccctct ccctgctgaa
    35521 agaagacggt atgcttattg ccgtcctcta tccgggacac gaaaacggca aacaggaggc
    35581 agaagccgtc ggacaatggg caaaaaacct gcctcaagaa cagtttgccg ttttacgtta
    35641 cggctttacc aaccggaaga atagcccacc ctatcttttg gcatttgaaa aactgcgtca
    35701 aaaataactg tttgcggtaa aataagccga ttctaaccct tattttgata cgagccgacc
    35761 atgcagtatc tgattgtcaa atacagccat caaatcttcg ttaccatcac cattttggta
    35821 ttcaacatcc gttttttcct actttggaaa aatccagaaa agcccttggt cggcttttgg
    35881 aaagcactgc cccacctcaa cgacacgatg ctgctgttta cgggattgtg gctgatgaag
    35941 attacccatt tctccccgtt caacgcgcct tggctcggca caaaaatcct gctcctgttc
    36001 gcctacatcg cactgggcat ggtaatgatg cgcgcccgtc cgcgttcgac caagttctac
    36061 accgtttacc tgctcgctat gtgttgcatc gcctgcatcg tttaccttgc caaaaccaaa
    36121 gtcctgccat tctgaaacac tgccatgaac aacagacatt ttgccgtcat cgccttgggc
    36181 agcaaccttg acaaccccgc acaacaaata cgcggcgcat tagacgcgct ctcgtcccat
    36241 cctgacatcc ggcttgaaca ggcttcctca ctgtatatga ccgcacccgt cggttacgac
    36301 aatcagcccg atttcatcaa tgccgtctgc accgtttcca ccaccttgga cggcattgcc
    36361 ctgcttgccg aactcaaccg tatcgaagcc gatttcggac gcgaacgcag tttccgcaat
    36421 gcaccgcgca cattggattt ggacattatc gactttgacg gcatctccag cgacgacccc
    36481 cgccttaccc tgccgcatcc gcgcgcgcac gaacgcagtt tcgtcatacg ccctttggca
    36541 gaaatcctcc ctgattttat tttgggaaaa tacggaaagg ttgtcgaatt gtcaaaacgg
    36601 ctgggcaatc aaggcatccg tcttttaccg gacaggtaat tccgcacgcg gatgccgtcc
    36661 gaacaccggt ttcagacggc atttttcctt tgccgccaac acgcgtgcaa aaaaaatcgc
    36721 cccttgagaa aggggcgcaa aaggaacaca aaccactacc aaaactttaa atctgcaaca
    36781 ctgcctgccg catactgtac ccgacaggat ataaggccct cactaaatcg tttcgagaaa
    36841 tccaaacttc ttcatcaccg acagaaaatc tgcctttctc cggcaccagc tccaacaaaa
    36901 acggctgaac cgccgtatgc agcttgtcct tacaccgccc cagctttctg gacaacgcgg
    36961 acagggcgcg tttgtgggca ttatccttgc ggtcgagctc cctcgcacta ccgacccagc
    37021 tcaaacgaag gttgcggctt tcatcttcaa tcaacaaaat acctgtttca acttccccca
    37081 cctcgggttc gcacgaaagc gcaatataat atttgccgtc aatattgaca tatgccgctt
    37141 catgcacctg acagttgcag ccggaagtgg caagcaacga ataaacagaa tcacggtgca
    37201 gcctcaaaat atcggaggaa atctgtttga aaatactttc cggcattttt ccctcctttc
    37261 gtctgctgcg ttgtttactg gattgactgc attatacaga catctcccaa ttttacaaat
    37321 gataattgtt cctatttttt aaaaagttta taaattattg ataaatatat gaattaattt
    37381 ttaagattta aaacctttgt ctcaaaaaat acaaaaatgc cgtctgaaac gtgttcagac
    37441 ggcattggct tcacaggcag tccgtttaaa atttcacgcc cttatgcagc gcgacgatgc
    37501 ccgcactcat attgtgataa tccacgctgt cgaagcctgc atccagcatc atctgtttca
    37561 aagtttcctg atcggggtgc atacggatgg attcggcgag atactggtag ctgtccgcat
    37621 ctttggcaat cagcctgccc attaccggca acagcttgaa agaataaaaa tcgtatgcgc
    37681 cttcgagcgg cttgtagatt ttggaaaact ccaacaccag caacgtaccg cccggtttca
    37741 aaacacggta catttcctta agcgcggcat ctttatacgt catgttccgc aagccgaacg
    37801 ccacggagac caagttgaaa taattgtcgg ggaaaggcag tttttccgca tcggcaagcg
    37861 acacgggtaa aatcatgcct tcgttcaaca gacggtcgcg cccgacggtc agcatagaag
    37921 aattaatatc ggtcagccaa acctcgcctt ccttgccgac ccgtttcgcc caaccgcgcg
    37981 acaaatcgcc cgtaccgccc gcaatgtcca acactttatc gccttttttc aggtgcgccg
    38041 tatggatggt aaaatgcttc catacgcggt gcagccctgc cgacatcaca tcattcataa
    38101 tgtcgtagtt tttggcgacg gagtgaaaca cttccgccac ttttccggct ttctcatctt
    38161 cgttgaccgt gctgaacccg aaatgcgttt tttgtccgcc catattctaa ccacctccct
    38221 gataccgccc tacggcatcc caaacttaac aatctgattg cttttcaaaa cgcgacgcgc
    38281 ccgccgccgc caatttgtcc aaatattcct gccacatcgc atcgtatccg tatgccagtc
    38341 tgtgcagata cgcccaatcg taaagaccgc tgtcgtgccc gtccgaaaaa ctgattttca
    38401 gcgcatactg tccgacaggc tgcaaatccg taatctgcac atccgcctta ccggtctgca
    38461 aaacatcctg tcccacgccg tgtccgcgca cttccgcact cggcgaatag acgcgtaaaa
    38521 attcggcagg cagattttta ggttcgtccc cataagtcaa aaccaacacg gcacggtttt
    38581 tcaacaaacg gatttcaaca ggaatattat tcaaaaaaca cctcggttcg gcatacaata
    38641 tccggcaggc gtacgcgtgc ctttaaacgg cgcgtatttt aacagcttta tcccgatacc
    38701 gatattcaaa cccacacttc agacggcatt gatatggaca atctcgtatg ggacggcatt
    38761 cccgacatcc gcacactcga ccaaaccatc cgcaaacacg cacacccgct caacctgatt
    38821 gtctgcctcc ccgataatca gattcccgat tttcaaaccg cacaagatgc ttcggactcg
    38881 gaatgccgtc tgaagcaccg tttggatcag gcaacccagt gcctccagtt cgacagcatc
    38941 aacctcatcg aacacatcct gcccgatgtc cgcttctggc tggttccccc ttcacgcacc
    39001 cgccgcctgc acgaacactt ccaccacatt tcctggcaga ccgaagccat cccgcaaacc
    39061 gaaagcaagt ccgacaaacc ctggtttgca cttccacaaa catccgaacg gaaaaaaccg
    39121 gaacacgtcc tcgtcatcgg tgcaggcatt gccggcgcat cgaccgccca cgccttagca
    39181 tcacacggca tttccgttac cgtattggaa gcccgaaaag ccgctcaagc cgccagcggc
    39241 aaccggcaag ggctgcttta cgccaaaatc tcgccgcacg acaccggaca gaccgaactg
    39301 ctgcttgccg gctacggcta caccaaacgc ctgctcggac acatcctgcc cgactccgac
    39361 acttggggcg gcaacggcat catccacctc aattacagcc gcaccgaaca acaacgcaat
    39421 cacgaattgg gtttgcaaaa acaccataac cacctctacc gcagcatcac gtctgcagaa
    39481 gccgaaaaaa tcgccggcat cccgctgaac acgccctacg ccgaaccatt atgcggactc
    39541 tactggcaac acggcgtatg gctcaatccg cccgcattcg tccgcaccct cctcagccat
    39601 ccgctgatcg aactatatga aaacacaacg ttaaccggca tttcccacga cggagaaaag
    39661 tggattgcaa gcacgccaaa cggcacattt accgccacac acatcatcta ctgcaccggc
    39721 gcgcacagcc cctgcctgcc cgaaaccaac ctcgccgccc tacccctcag gcaaatacgc
    39781 ggacaaaccg gactcacacc gtccaccccg ttttccgaac aactgcgttg cgccgtttca
    39841 ggcgaaagct acatcagccc gtcgtggcac ggactgcact gctacggcgc gagttttatt
    39901 cccaacagca gcaataccgg atggaacgaa gccgaagaag cctcaaaccg ccaagcattg
    39961 gcacacctta accccgccct tgccgaatca ttgtttgccg ccaacccaaa cccccaaaaa
    40021 caccaagggc acgccgccat acgctgcgac agccccgacc acctccccgt cgtcggcgca
    40081 ctcggcgaca ttgccgccat gcggcagact tacgccaaac ttgcactgga caaaaactac
    40141 cgcatcgaca ccacatgccc atatctgccc aatgcctaca ccaataccgc ccacggcaca
    40201 cgcgggcttg ccaccgcccc catctgcgcc gccgccgttg ccgccgaaat cctaggcttg
    40261 ccccatctct tttcacaacg cctgcgccac gccctacacc ccaaccgcac cgtcatccgc
    40321 gccatcgtca gaaggcagaa cctgattcct taagcctttg ccgcatgccg tcccgaccac
    40381 aaaaaatcat attcaaataa acataccaat cctgacggta aaatatcgaa aacacccata
    40441 acgttttctt cccaaaataa ggagtaagga atgcctaaca aattgccttt gctgtccaag
    40501 atgaattttc cgtaacattc gaagacttcc ttaaccctgc cagccgcttc ggtttctgta
    40561 ccccctgtta gataaggaag ggatgaaaaa gccctgttac ttgtatgaag caaagggcgt
    40621 taatttttgt taatcttccc ttctcaggaa ctcaatatat aaggatgtta atttttattt
    40681 cccttctcga ggactcaata g
//
LOCUS       DAXDSD010000002        40247 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXDSD010000002 DAXDSD010000000
VERSION     DAXDSD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40247
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.002"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            195..1538
                     /locus_tag="ACFB2Q_000049"
     CDS             195..1538
                     /locus_tag="ACFB2Q_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC0917178.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRGKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1541..2773
                     /locus_tag="ACFB2Q_000050"
     CDS             1541..2773
                     /locus_tag="ACFB2Q_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC0917179.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2766..3542
                     /locus_tag="ACFB2Q_000051"
     CDS             2766..3542
                     /locus_tag="ACFB2Q_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC0917180.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIRPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGVQ"
     gene            3542..4168
                     /locus_tag="ACFB2Q_000052"
     CDS             3542..4168
                     /locus_tag="ACFB2Q_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC0917181.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4172..4765
                     /gene="nqrE"
                     /locus_tag="ACFB2Q_000053"
     CDS             4172..4765
                     /gene="nqrE"
                     /locus_tag="ACFB2Q_000053"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC0917182.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYDFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4779..5996
                     /gene="nqrF"
                     /locus_tag="ACFB2Q_000054"
     CDS             4779..5996
                     /gene="nqrF"
                     /locus_tag="ACFB2Q_000054"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC0917183.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNDEKEL
                     TMPAGGKLLGALASQGIFVPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLHSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6513..7568
                     /locus_tag="ACFB2Q_000055"
     CDS             6513..7568
                     /locus_tag="ACFB2Q_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC0917184.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILQQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNAHGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVSDSAMTADGLSTGLFVLGETEALRLAEQEKLAVFLIVRD
                     KDGYRTAMSSEFAKLLR"
     gene            7594..7809
                     /gene="nqrM"
                     /locus_tag="ACFB2Q_000056"
     CDS             7594..7809
                     /gene="nqrM"
                     /locus_tag="ACFB2Q_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC0917185.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7933..8511)
                     /gene="grpE"
                     /locus_tag="ACFB2Q_000057"
     CDS             complement(7933..8511)
                     /gene="grpE"
                     /locus_tag="ACFB2Q_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC0917186.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8694..9512
                     /gene="cysE"
                     /locus_tag="ACFB2Q_000058"
     CDS             8694..9512
                     /gene="cysE"
                     /locus_tag="ACFB2Q_000058"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC0917187.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARLGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            complement(9716..11227)
                     /gene="ubiB"
                     /locus_tag="ACFB2Q_000059"
     CDS             complement(9716..11227)
                     /gene="ubiB"
                     /locus_tag="ACFB2Q_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC0917188.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCVSLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGKRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HVESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(11262..11719)
                     /locus_tag="ACFB2Q_000060"
                     /pseudo
     CDS             complement(11262..11719)
                     /locus_tag="ACFB2Q_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(11716..11862)
                     /locus_tag="ACFB2Q_000061"
     CDS             complement(11716..11862)
                     /locus_tag="ACFB2Q_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917189.1"
                     /translation="MPSERSDGIFTFGKYIIGMNLHIIRLLEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(11879..12217)
                     /gene="erpA"
                     /locus_tag="ACFB2Q_000062"
     CDS             complement(11879..12217)
                     /gene="erpA"
                     /locus_tag="ACFB2Q_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC0917190.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(12350..13036)
                     /locus_tag="ACFB2Q_000063"
     CDS             complement(12350..13036)
                     /locus_tag="ACFB2Q_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC0917191.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTV"
     gene            13223..13495
                     /locus_tag="ACFB2Q_000064"
     CDS             13223..13495
                     /locus_tag="ACFB2Q_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917192.1"
                     /translation="MKKTSKYLIYTAAFTSFCFAFQENRSEAKQPDITLSASLCEQFN
                     MLNAKDMDTEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGVVYE"
     gene            13703..15631
                     /gene="dnaK"
                     /locus_tag="ACFB2Q_000065"
     CDS             13703..15631
                     /gene="dnaK"
                     /locus_tag="ACFB2Q_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC0917193.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            15854..17774
                     /locus_tag="ACFB2Q_000066"
                     /pseudo
     CDS             15854..17774
                     /locus_tag="ACFB2Q_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            17831..18808
                     /locus_tag="ACFB2Q_000067"
     CDS             17831..18808
                     /locus_tag="ACFB2Q_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFC0917194.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLTAGKEQFTVYGAPAMPSVT
                     IAVTALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTNGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVHGFDLVKAWGQAFGT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
     gene            19145..19282
                     /locus_tag="ACFB2Q_000068"
     CDS             19145..19282
                     /locus_tag="ACFB2Q_000068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917195.1"
                     /translation="MNEDKYRENMTWDEIENVVGTLVSEERMLKSNSFLSIKNSKNRL
                     A"
     gene            19361..20131
                     /locus_tag="ACFB2Q_000069"
                     /pseudo
     CDS             19361..20131
                     /locus_tag="ACFB2Q_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20158..20850
                     /locus_tag="ACFB2Q_000070"
     CDS             20158..20850
                     /locus_tag="ACFB2Q_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC0917196.1"
                     /translation="MLCLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            21042..21656
                     /locus_tag="ACFB2Q_000071"
     CDS             21042..21656
                     /locus_tag="ACFB2Q_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC0917197.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIYIGQY
                     LGSVILILVSLFLAFVLNYVPEKWVLGLLGLIPIYLGIKVAIYDDCEGEKRAKKELDE
                     KGLSKLVGIVALVTVASCGADNIGLFVPYFVTLDLVDLLVTLLVFLILIFVLVYTAQR
                     LANISGVGEIVEKFSRWIMAVIYIGLGLFIIIENNTIRTIISII"
     gene            21952..23047
                     /locus_tag="ACFB2Q_000072"
                     /pseudo
     CDS             21952..23047
                     /locus_tag="ACFB2Q_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
     gene            complement(23094..25283)
                     /locus_tag="ACFB2Q_000073"
     CDS             complement(23094..25283)
                     /locus_tag="ACFB2Q_000073"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC0917198.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTGQQKASDEIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            25423..26208
                     /locus_tag="ACFB2Q_000074"
     CDS             25423..26208
                     /locus_tag="ACFB2Q_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC0917199.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQQ"
     gene            complement(26307..28241)
                     /locus_tag="ACFB2Q_000075"
     CDS             complement(26307..28241)
                     /locus_tag="ACFB2Q_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC0917200.1"
                     /translation="MSLIECKNINRCFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGMGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIRRLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSMGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(28307..29485)
                     /locus_tag="ACFB2Q_000076"
     CDS             complement(28307..29485)
                     /locus_tag="ACFB2Q_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC0917201.1"
                     /translation="MAKMMKWSAVAAVAAAAVWGGWHYLKPEPQASYITETVRRGGIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTTQTNTLDMEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESDLGYTRITATMDGTVVAIPVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLLIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            29711..30100
                     /locus_tag="ACFB2Q_000077"
     CDS             29711..30100
                     /locus_tag="ACFB2Q_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC0917202.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLIAYPSYKTYIRRARLSEVK
                     STLLVNAQNLERYYRQKGTFEKYDSTKLKQNKYFKITLIKASPDHFTLQAAPDTATNE
                     GETCTVTLNDGGTIAASGTNQSCPGFD"
     gene            complement(30194..33679)
                     /gene="smc"
                     /locus_tag="ACFB2Q_000078"
     CDS             complement(30194..33679)
                     /gene="smc"
                     /locus_tag="ACFB2Q_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC0917203.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQVEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPGLSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDVLEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            34088..34756
                     /locus_tag="ACFB2Q_000079"
     CDS             34088..34756
                     /locus_tag="ACFB2Q_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917204.1"
                     /translation="MGLRQKLRGIIPNLATSIGTSAEANAPGALALRGSSEASKKFSI
                     AEGYLASSDGYGAIAIGSAAKIKQLEKGTINHIVGNDNKGLYVDADGNVTKITVRTES
                     EKDILSRYGQTYGAVALGFRSSSHNLFASSFGAFSTATAIESLAVGDSSQSTGYRSAT
                     FGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRKIVNVADG
                     TEDL"
     gene            34728..35213
                     /locus_tag="ACFB2Q_000080"
     CDS             34728..35213
                     /locus_tag="ACFB2Q_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFC0917205.1"
                     /translation="MSPMARKIYDAVNVRQLNRLSKRTNRVGASAAALASLKPAQLGK
                     NDKFAFSLGFGSYKNAQAVAMGAVFKPAENVLLNVAGSFAGPDRAFGAGVSWKFGGKP
                     TPAVAAQNAAHPAKVLQLRQEVAALRARQAETDRKLHKQAEMENELQQLRRALSELKK
                     H"
     gene            35422..36144
                     /gene="lpxH"
                     /locus_tag="ACFB2Q_000081"
     CDS             35422..36144
                     /gene="lpxH"
                     /locus_tag="ACFB2Q_000081"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC0917206.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            36413..37999
                     /locus_tag="ACFB2Q_000082"
     CDS             36413..37999
                     /locus_tag="ACFB2Q_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC0917207.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(38165..38368)
                     /locus_tag="ACFB2Q_000083"
     CDS             complement(38165..38368)
                     /locus_tag="ACFB2Q_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC0917208.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            38484..38981
                     /locus_tag="ACFB2Q_000084"
     CDS             38484..38981
                     /locus_tag="ACFB2Q_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC0917209.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            39063..>40247
                     /locus_tag="ACFB2Q_000085"
     CDS             39063..>40247
                     /locus_tag="ACFB2Q_000085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC0917210.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVK"
BASE COUNT         9635 a        11088 c        10129 g         9395 t
ORIGIN      
        1 caaatcaata tcccccttcc gacaattaaa aacggcttcc gcaaggaaaa aagcggcaaa
       61 tctgacaaac aggtacaatt cctgataaaa tctaacactt tgctacatta atttattcaa
      121 tgcttttgat gccacgcgac acggtatcaa aaacacgccg tttttacctt aaccatcaca
      181 agtaatgcaa atctatgatt aaaatcaaaa aaggtctaaa tctgcccatc gcgggcagac
      241 cggagcaagt catttatgac ggcccggcca ttaccgaagt cgcgttgctt ggcgaagaat
      301 atgtcggcat gcgcccctcg atgaaaatca aggaaggtga agccgtcaaa aaaggccaag
      361 tgctgtttga agacaaaaag aatccgggcg tagtatttac tgcgccggct tcaggcaaaa
      421 tcgccgctat tcaccgtggc gaaaagcgcg tacttcagtc agtcgtgatt gccgttgaag
      481 gcaacgacga aatcgagttc gaacgctacg tacctgaagc gctggcaaaa ttgagcagcg
      541 aagaagtgcg ccgcaacctg attcaatcag gcttatggac tgcgcttcgc acccgtccgt
      601 tcagcaaaat ccctgccgta gatgccgagc cgttcgccat cttcgtcaat gcgatggaca
      661 ccaatccgct ggctgccgac cctacggtca tcatcaaaga agccgccgaa gacttcaaac
      721 gcggcctgtt ggtattgagc cgcctgaccg aacgtaaaat ccatgtgtgt aaagcagcag
      781 gcgcagacgt gccgtctgaa aatgctgcca atatcgaaac acatgaattt ggcggcccgc
      841 atcctgccgg cttgagtggc acgcacattc atttcatcga gccagtcggc gcgaataaaa
      901 ccgtgtggac catcaattat caagacgtga ttgctatcgg acgtttgttc gtaacaggcc
      961 gtctgaatac cgagcgcgtg gttgccttgg gcggcctgca agtcaacaaa ccgcgcctct
     1021 tgcgtaccgt tttgggtgcg aaggtgtctc aacttaccgc cggcgaattg gttgacgcgg
     1081 acaaccgcgt gatttccggt tcggtattga acggtgcgat tgcacaaggc gcgcatgatt
     1141 atttgggacg ctaccacaat cagatttccg ttatcgaaga aggccgcggc aaagagctgt
     1201 tcggctgggt tgcgccgcag ccggacaaat actccatcac gcgcaccact ctcggccatt
     1261 tcctaaaaaa caaactcttc aagttcacga cagccgtcaa cggcggcgac cgcgccatgg
     1321 taccgatcgg cacttatgag cgcgtaatgc cgttggacat cctgcctacc ttgcttttgc
     1381 gcgatttaat cgtcggcgat accgacagcg cgcaggcttt gggttgcttg gaattggacg
     1441 aagaagacct cgctttgtgc agcttcgtct gcccgggcaa atacgaatac ggcccgctgt
     1501 tgcgcaaagt gctggaaacc attgagaagg aaggctgatt atgggcttaa aacattttct
     1561 ggaaaaaatc gaaccgcact tcctgccggg cggcaaacat gaaaaatggt atgccctcta
     1621 tgaagctgcg gcgacgattt tctatacatc cggcgcggta acgcgcaaag cggcgcacgt
     1681 ccgcgacgcg ctcgactcca aacgcatgat gattttggtg tggctggctt tgttccccgc
     1741 catgttttac ggcatgtaca acgtcggcgc acaggcattc ggtgccttaa cgcccgattt
     1801 gctgcaacaa agcatcgcca acgactggca ttacgccctt gccaacgctt tgggcatcaa
     1861 tatgtcgcct gaagcgggcg tgttgggcaa aatgctgttc ggcgcgattt acttcctgcc
     1921 gatttacgcg accgtattta ttgtgggcgg cttctgggaa gtcttgttcg catccgtacg
     1981 caaacacgaa atcaacgaag gtttcttcgt tacttcgatt ctgtttgcct taatcgttcc
     2041 gcccacgctg ccgctgtggc aggcggcttt gggtatttct ttcggcgttg tggttgcgaa
     2101 agaagtattc ggcggtacag gtaaaaactt catgaaccct gcgctggcag gccgcgcctt
     2161 cctgttcttc gcctaccccg ccaacttgag cggcgatgcg gtttggacgg cggttgacgg
     2221 ctattccggc gcaaccgcgc tggcgcaatg ggcggcacac ggtgcagacg gcctgaaaaa
     2281 cgccgtaacc ggtcaaacca tcacttggat ggacgcattt atcggcaaac tgcccggctc
     2341 catcggcgaa gtctccactt tggcactctt aatcggcggc gcgtttatcg tgtttgcccg
     2401 catcgcttct tggcgcatta ttgccggcgt gatgatcggt atgattgcga tgtcttcgct
     2461 gtttaacttc atcggttctg acaccaacgc tatgtttgct atgccttggt actggcactt
     2521 ggtggtcggc ggcttcgcca tcggtatgct gtttatggcg accgaccccg tttccgcttc
     2581 ctttaccaat gtcggcaaat ggtggtacgg cgcgcttatc ggcgtaatgt gcgtgttaat
     2641 ccgcgtggcc aatccggctt accccgaagg catgatgttg gcgattctgt ttgccaacct
     2701 gtttgccccg attttcgact atttcgtcgc acaagcgaac atcaaacgca gaaaggcgcg
     2761 cagcaatggc taagaaattc gataaagaca gcttcagcgg cacgctgatt gtcgtgttgg
     2821 cggtcagcct gatttgctcg gtcatcgttg ccggtgcggt cgtcggcttg aaacccatcc
     2881 aagaaaaaca aaaactccaa gacaaacaag gctatatttt gagcgtggcc ggtttgatgg
     2941 ataaggacac cgacatcggt aaaacctttg ccgagcgtat cgagcaacgc gtggtcgatt
     3001 tggcgaccgg cgaatatgtg aaagacgcgc cgaaagactt cagcgcgcgc atcgcaggca
     3061 aagaccccgc gcaaagcatc cgcatcaaac ctgaagacga tttggcaggc atcaaaagcc
     3121 gcgccaaata caccgaggtt tatttggtaa aaggcgaaga cggcaaaatc gggcaaatca
     3181 tcctgccgat gcacggcaac ggtttgtggt cggtgatgta cggcttcgtc gccatccgac
     3241 ccgacggcaa caccatcaac ggcattacct actacgaaca aggcgaaacc ccgggcttgg
     3301 gcggcgaaat cggcaatcct ttgtggcagc aaaaattcgt cggcaaaaaa ttgtttgacg
     3361 gacaaggcaa actcgccctg cacgtgggca aaggcgcggg ttcggacaaa gaacacggcg
     3421 tagacgccct ctccggcgcg tcgctgactt ccaaaggcgt gcaaggttcg ttcgcctact
     3481 ggttcggcga aaacggctat atcccctacc tgaacaaatt gaaatcagca ggagtgcaat
     3541 aatggctgat atgaaacgct tgaaacattt gatgttctca ccctttatcg acaacaaccc
     3601 gattgccttg caggttttgg gtatttgttc ggctttggcg gttaccacca aacttcagac
     3661 ggccatcgta atgggtattt ccgtagcttt ggtaaccggt ttttccagct tcttcatttc
     3721 gctggtgcgc aactacatcc ccaacagcat ccgcatcatc gtgcaaatgg cgattatcgc
     3781 gtcgctggtt acgctggtcg accaactgct tcaggcattt gcctacgaat tgtccaagca
     3841 gctttccgta ttcgtcggcc tgattattac caactgtata gtgatgggcc gtgccgaagc
     3901 atttgcgatg aaagagccgc cgctggaaag cctgatcgac ggcatcggca acggtgcggg
     3961 ctacgggatg atgctgcttg tcgtcgccac cgtccgcgaa ctgatcggct caggcaaact
     4021 tttaggttat accgtctttc aaaccgtaca ggacggcggc tggtatcaga ccaacggcct
     4081 cttcctactc gcccccagcg cgttcttcat catcggcttt ttgatttggg gactgcgtac
     4141 ttggaaaccc gaacaggcgg aggaataagc tatggaacac tatttgagcc tcttcatcaa
     4201 atccgtcttc attgaaaata tggcgctatc cttctttttg ggtatgtgta cgtttttggc
     4261 ggtatccaaa aaagtatcca ccgcattcgg tttgggtgtg gcggtaattt tcgtactcgg
     4321 gctgtccgtc cccgccaacc aattggttta ctcgctgctc aaagacggcg cgattgtcga
     4381 aggcgtggat ttgacctttt tgaaattcat cacctttatc ggcgtaattg ccgctttggt
     4441 gcagattttg gaaatgttct tggacaaatt cgtccccgcc ctctataacg cgctgggcat
     4501 ctacctgcct ctgattaccg taaactgcgc gattttcggc gccgtatcgt ttatggcgca
     4561 acgcgaatac gacttcggcg agtccgtcgt gtacggcttc ggcgcgggtt tgggctggat
     4621 gttggcgatt gtcgctttgg cgggcattac cgaaaaaatg aaatattcgg acgctcccaa
     4681 aggcctcaaa ggcttgggca ttacctttat cgccgccggc ctgatggcaa tggcgtttat
     4741 gtcgttctcc ggcatccagt tataagaaag ggaccggtat ggagattatt ttaggtatcg
     4801 tgatgtttac cgtcatcgtc ttagctttgg cactgatgat tctgtttgcc aaatccaagt
     4861 tggtcagcga aggcgacatc accatcaaag tcaatgatga aaaagaactg actatgcccg
     4921 ccggcggcaa actcttgggc gcgcttgcca gccaaggcat ctttgtcccc tccgcctgcg
     4981 gtggcggcgg ttcgtgcgga caatgccgcg ttgtcgtgaa aagcggcggc ggcgacattc
     5041 tgccgaccga gctgtcccac atcagcaaac gcgaagcacg cgaaggctgc cgtttgtcct
     5101 gccaagtcaa tgtcaaaacc gacatggaca tcgaagtacc cgaagaagtg ttcggcgtga
     5161 aaaaatggga atgcaccgtc atttccaacg acaacaaagc aacgttcatt aaagaactca
     5221 agcttgccat tcccgaaggc gaagaagttc ctttccgcgc cggcggctac attcaaatcg
     5281 aagccccgcc gcacaccgtt gcctacaaag acttcgacat tcccaaggaa tatcacgaag
     5341 actgggacaa atacaatctg tggcaatacg tttccaaagt ggacgagccg attttgcgcg
     5401 cctactctat ggcttcgtat cctgaagaaa aaggcatcat tatgctgaac gtgcgtatcg
     5461 ccacgcctcc cccgcgcgta cccgatgcgc ctccgggaca aatgtcgtcc tacatctggt
     5521 cgctcaaacc cggcgacaaa gttacgattt ccggcccgtt cggcgaattt ttcgccaaag
     5581 acaccgatgc cgaaatggta tttatcggcg gcggtgcggg tatggccccg atgcgttccc
     5641 acattttcga ccagttgaaa cgtttgcact ccaaacgcaa aatcaccttc tggtacggcg
     5701 cacgctccaa acgcgaaatg ttctatgtcg aagacttcga ccaactcgcg gcagaattcc
     5761 cgaacttcac ttggcacgtc gccctgtccg acccattgcc tgaagacaac tgggacggct
     5821 acacgggctt catccacaac gtggtttacg aaaaccacct gaaaaaccac gaagcaccgg
     5881 aagactgcga attttatatg tgcggcccgc cgattatgaa ccagtccgtc atcaaaatgc
     5941 tcaaagactt gggcgtggaa gacgaaaaca tcctcttgga cgatttcggc ggttaaatat
     6001 tcggcttcaa acacaaaagg aagaaacccc ggtttcttcc ttttttatcc gatgccgtcc
     6061 gaacaccctt cagacggcat ccgcccatac ccgtatcaat atcgtttttt taccctgcgg
     6121 cggatgtgcg gctaaatatt ttgacgaaaa aacaaagaag tatacttctt tttagttatg
     6181 gttgattcca taactaaaaa agtatcgatc aacccatttt acgaggtacg gataatgtct
     6241 gacagctttg aaaacagccc cgaatacgat gattggattg aatcgggcgg tcgtgatgaa
     6301 gattacgaat actactacaa caaatggcaa cgcaggacgc gctgaccatt aaaccctgac
     6361 aaaccggttt gtaaaacacc ggtttgtttt ttattaccgc cacccaaacg acgggcgatg
     6421 ccgcccgaac gcccttcaaa cggcatcggc atagaaacat accggccgtt ttgcgatata
     6481 attcaagcca tttgccaaaa ccgtcaaaca ctatgccgtc tgaaacacgc ctgccgaacc
     6541 ttatccgcgc cttgatattt gccctgggtt tcatcttcct gaacgcctgt tcggaacaaa
     6601 ccgcgcaaac cgttaccctg caaggcgaaa cgatgggtac gacctatacc gtcaaatacc
     6661 tttcaaataa tcgggacaaa ctcccctccc ctgccaaaat acaaaagcgc attgatgatg
     6721 cgcttaaaga agtcaaccgg cagatgtcca cctaccagac cgattccgaa atcagccggt
     6781 tcaaccaaca cacagccggc aagcccctcc gcatttcaag cgatttcgca cacgttaccg
     6841 ccgaagccgt ccgcctgaac cgcctgactc acggcgcact ggacgtaacc gtcggccctt
     6901 tggtcaacct ttgggggttc ggccccgaca aatccgttac ccgtgaaccg tcgccggaac
     6961 aaatcaaaca ggcggcatct tatacgggca tagacaaaat cattttgcaa caaggcaaag
     7021 attacgcttc cttgagcaaa acccacccca aagcctattt ggatttatct tcgattgcca
     7081 aaggcttcgg cgttgataaa gttgcgggcg aactggaaaa atacggcatt caaaattatc
     7141 tggtcgaaat cggcggcgag ttgcacggca aaggcaaaaa tgcgcacggc gaaccgtggc
     7201 gcatcggtat agagcaaccc aatatcatcc aaggcggcaa tacgcagatt atcgtcccgc
     7261 tgaacaaccg ttcgcttgcc acttccggcg attaccgtat tttccacgtc gataaaaacg
     7321 gcaaacgcct ctcccacatc atcaatccca acaacaaacg acccatcagc cacaacctcg
     7381 cctccatcag cgtggtctca gacagtgcaa tgacggcgga cggtttatcc acaggattat
     7441 ttgttttagg cgaaaccgaa gccttaaggc tggcagaaca agaaaaactc gctgttttcc
     7501 taattgtccg ggataaggac ggctaccgca ccgccatgtc ttccgaattt gccaagctgc
     7561 tccgctaaac atacaaaccg attaggaaac accatgaaaa ccctgctcct caccttcggc
     7621 atcttcctga ccgtcatcat cggtatggcg gtcggctata ttttctccaa acgcaccatc
     7681 aaaggcagtt gcggcggcat taccgcttta ggtatgaaaa aaatgtgcga ctgcgacaca
     7741 ccttgcgaca ccctgcaaaa aaagctggat gaagaaaaac aggcaggcgg catacgggtt
     7801 gaccgttaac gccgaaacaa aatgccgcct gaagcccggc cggcgggttc agacggcatt
     7861 gccgctttac ttcgatcggt ttatacgcaa aacgcgcttc aggaaataaa tcagattatt
     7921 ccccagacgc cttcaggctt ccttctgtgc caccgtaacc atagccgggc gcaacacacg
     7981 gtcggacagg gtataaccct tcttcatcac accaaccacg gtattgggtt cctgctcact
     8041 ggccaccgcc tgcatcgcct gatggatatt cggatcgagc ttatcgcccg ctttaggatt
     8101 gatttccttg atttgcgtgg catcaaacgc cttctgcaac tcattcaaag tcatctgcac
     8161 gcccattttc agcgcatcga aattaccgct ctgatccaaa agcgccattt ccagataatc
     8221 cttgaccggc aacatttcca cggcaaactt ctgtccggcg aacttgtgcg tatcggcaat
     8281 ttcctgctgg tggcggcggc gcaggttttg ctcgtttgcc aaagcgcgca gttgttcgtc
     8341 tttcaactgc gcttccaact cggcaatccg cgcctgcaaa tcctcataag ccggctcggc
     8401 ggcagcctgt tcctgtacac cgtccgcatt tcctactgtc tcgacggttt ccaccgcctc
     8461 cacattttca accgcttctt cactgttttg ctgctgtgtc tgttcgctca tatcgtatcc
     8521 ctcaaaacaa attggaaatc aaaatccggt tattacccga gcaagataag gacattttca
     8581 agaaacttca agcaaaggca ggaaatttca catccgccgc cgaataccct accgcaaaaa
     8641 ccatataaac ggtaagatac cgccgacctt atgcttttca gacggcatcc attatgaaaa
     8701 aaaaccattt gaacacaacc ggtttcgacc tctggcacac catccgcgaa gaaaccgcgg
     8761 ccgctgccgc cgccgaaccg atgctggcaa gttttttgca ccaaaccgtg ttgcgccacg
     8821 agtccctcgg ctccgtcctt gcctaccacc tttccagcaa actcggcagc ccgattatgg
     8881 acgtgcgcgc gctgtttgaa atttaccagc aggcattggg cagcgacacc caaatcagca
     8941 aatgtgtcga agcagactta aaagccatct acgaacgcga tcccgcctgc gacgaatatt
     9001 cgctgccgct tttatatttc aaaggcttcc acgcgattca ggcacaccgc atcaaccacc
     9061 ggctgtatct cgacggacgc aaaacgctgg cgtatttcct acaaaaccgt atgtccgaag
     9121 tattcggcgt ggacatccat cccgccgccc gtttaggata cgggctgatg ctcgaccacg
     9181 ccaccggctt tgttgccgga gaaaccgccg tgttgggcaa caatatttcg attttgcacg
     9241 gcgtaacgct cggcggttcg ggcaaagaag gcggcgaccg ccaccccaaa atcggcgacg
     9301 gcgtgatgat cggcgcaaac gcctcgatat tgggcaatat ccgcatcggc agcaatgcca
     9361 aaatcggcgc gggcagcgtc gtggtttcag acgtgccgcc gtccatcacg gttgtcggcg
     9421 tacccgccaa acccgtggcg cgatcgctca aaaccccgtc ggcggatatg gatcaaaata
     9481 tccagtttgc cgaaatcgac tttatgattt gaatatccgc accaatgccg tctgaaacgc
     9541 caaacgggct tcagacggca ttgcccgctt caagcctgcg ccttaatatg ccgccgatgc
     9601 cggcacttat cagtcggaca aaatgccttt tattcgatac gcccgattcc cacgcaaccg
     9661 gaaataaccg gtttccttgt aaacgggcgg agtcgggcaa tgattcggac aaagcctatt
     9721 tcaaaagcaa aatcagcagc aaaacaaccg ctatcgctgc cagccacagg ctttgccgct
     9781 gctgcacttt gaccaaatgg atataggcat cgcgcatttc ctgccggcgc gcctcatcga
     9841 ccaaggcatt gattttgcgc ggcagggaag ggatgatttg cgcccagtct ggggcttcgt
     9901 ttttgaggtt gcgccaaagg gctttgggac cgacctgccc gttcatccat tttaccaaaa
     9961 acggtttggc ggttttccac aagtccaaat cgggatcgag ttggcggccc aagccttcga
    10021 tgttgagcag cgttttttgc agcaatacca gctgcggctg gatttcgaca ttgaagcggc
    10081 ggctgacttc aaacaggcgc atcagtacca agccgaagga aatctgcgaa atcggtttgt
    10141 tgaacacagg ctcgcacacg gcgcgaaccg ccgcttccaa ctcttccgca cgcgtgtcgg
    10201 caggcaccca gcccgattcg acgtgggcgg tggcgacacg tcggtaatcg cggttgaaaa
    10261 aggcgaggaa attgatggcg agatagcgtt tgtcgtaatc ggtcagcgta ccgacgatgc
    10321 cgaaatcaag ggcgatatag cggttgtcgg cggcaaccaa aatattgccg gggtgcatat
    10381 ccgcgtggaa aaagccgtcg cggaaaactt gcgtgaaaaa gatttccacg ccgtaatcgg
    10441 caagtttgtg caaatcgatg ccgtctgctt tgagtttggc gatgtcggaa accggcgtgc
    10501 cgtccatcca ttcaatcgtc agcacgtcgc ttgtgcagta gtcgtaaaac accttgggca
    10561 caatcagcat attgctgttt tggaaattgc gcccgagttg tcctgcattg gcggcttcgc
    10621 gcatcaaatc caattcgtcg tgcaggtatt tgtcgaactc cgccaccact tcgcgcggct
    10681 tcagacgctt gccgtctgaa aacaagcgtt cgacccaagc cgcgccgaaa cgcatcagcg
    10741 acaagtcttg ttcgataagg ggcaagaggt tggggcgcaa aactttgacc gccactcgtt
    10801 cgcccgaatg caggcgggct ttgtgtactt gggcgatgga cgcgctggcg acgggctcgg
    10861 tttcaaattc cgcatacagc ttttcgatgg gctgacccag cgatttttcg atttgttcac
    10921 gcgaaagccg cgcgtcaaaa ggcggcactt tgtcttgcag ctttgccagt tcgacggcgt
    10981 aatcgtgcgg aatcaaatcg gggcgtgtgg acaaaacctg cccgaacttg atgaaaatcg
    11041 gccccaggct ttccaaggca aggcgcagac ggacggcagg cggttcgttt ttcaatttgg
    11101 acgactgcgg catcattttc agcagagcgc atatccaacc gctacccatc agcgaaacgc
    11161 acagacctgc cagccgatag cggtaaaaag tcccgacaat gaccgtcagg cgtttcaacc
    11221 atttcatata taaaatcccg aatacagcat ttatcggaca atcaaaaaat cttaccgagc
    11281 agatagccca aaaaggcaat cagcaacccg aaaaccagcg gtttgatatt gcggggcggc
    11341 tcgggttgcg aagatgcgga aatatcatct tcccgcccgc cgtgaagttc aagatattcg
    11401 ggcatttccc catccgcgcc tcctccttcg ccctccccct cctccggcgg cacgaccgtt
    11461 tccaactcca tatacgggta ataatatcca aacccaaata gctgaaataa tcatgcaact
    11521 cttcccgttt cgcccaagtg catttttcct caaccagcac ttgcggtttg gaatcgagcg
    11581 attcgatcaa tccttccgcc ttttcttcgc cgagattctc cttaaggcac tcccggataa
    11641 gcccgcgatc cacttggggc gggtaggaaa catatacatc gtacaaatcg tttcggtaat
    11701 ctttactcat ctcaatcata agggaacaaa tacgcctgat accgttccgc ccgttccttt
    11761 cgggaaaccc gattcatctt caagcaatcg gattatatgt aaattcatcc cgataatata
    11821 ttttccaaaa gtaaaaatgc cgtccgaacg ttcagacggc ataacacaaa ccagccggtc
    11881 acacggaaaa cgacgatccg caaccgcagg ttgtttccgc gttcggattg cggatgacga
    11941 actgcgaacc ctgcagactt tccgtatagt cgatttccgc accgaccaga tattgatagc
    12001 tcatcggatc gaccaaaaag accaaaccgt ttttctcaat ttcaaaatcg tcgtcgtttt
    12061 tgatttcgtc aaaagtaaat ccgtactgga aacccgaaca gccgccgccg ttgacaaaaa
    12121 cccgcaattt caaatcggga ttgttttctt cggcaatcaa atcggcaact ttggcacagc
    12181 agctgtcagt aaaaataata gggctttcgt ccgacatagt gttattcctt aatataatat
    12241 tttctgcatt ctctctcaac cccccttcaa aggcaagcag ggcttcgcct ttgaaaaagg
    12301 aattgtccca atagataagt ttgaccgcct gcaatttcaa gccccaaact caaaccgtcc
    12361 tgccgaacca ctcgacacgc ccgataatct ccacatcatc ggtcaagtcg ttcaaattga
    12421 tttcaaaagc aggataagcc tcgttggcgg aaatgacatt gataatcccg ccggggacga
    12481 tttgcaggcg tttgaccaac agattttcat taatccgcaa cacatacagt ccgtctctcg
    12541 gcgtattttc cccatgattg accaaaatcg aatctccgtc attcaaaacc ccctccatcg
    12601 aatccccctt gacggaaatt acagacaggt ttttcgtatc tcgggtaaca taattctcaa
    12661 tccaatgccg tctgaacgcc attgtaaata ccggttcctc atgaccgaca aactgcccgt
    12721 atcccgcagc cgcccgaata tcatatctcg gcacaaagac aaactcgtcc gtatcaactt
    12781 catttcccaa ggtatcgtaa gcaagggatt tttttggggc ttcatccgga aacggattac
    12841 cctcccctgt cagcagccaa tcgatactac accccttcaa ctgcttaatt tttttcaatg
    12901 tttcagactt cggcagaccg ccttcattcc atattctgct gaagcccgca atcgtcattt
    12961 caatatcgga tgcgattttt gcctgcctcg cttcactttt ccataaaaaa accagtctgt
    13021 ctttaaaggt ttccataaat atccttccgc tccccttcat tcaatcatct aaagtaaaca
    13081 tgaaatatat tttacattag ctatcccaaa aacggaataa ctatgataaa ctaaatttaa
    13141 attatgattt ttcttatcca atacataatt tttcagattt tccataggtt taatcccgaa
    13201 ttgacagaaa aaggatagaa aaatgaagaa aaccagcaaa tatcttatct atactgcggc
    13261 atttacctca ttctgctttg ccttccaaga aaaccgttct gaagccaaac agcccgacat
    13321 cactttatcc gcatccctgt gcgaacaatt caacatgctg aacgccaaag atatggatac
    13381 agaacaagtc tccctttcca aagaatgcga catcatcgag tcttcacacg actgggaaaa
    13441 agagtacggc aacttgaacg aacaggaaat gctcgccggc gtcgtctatg aataaacctg
    13501 cctgccattt gaaacattat gcttgaatgc attggagcca aatgtattaa atcaaatata
    13561 aaaccaatat attcataaag ttatatactt atagccatgc tgtagcttga aacagcccgc
    13621 cgcccgccct atttacagcc catcgggaca aaatgttcca acatatttct caaaataagc
    13681 aaaatcaaat aggagtatcc acatggcaaa agtaatcggt atcgacttag gtacaaccaa
    13741 ctcttgtttg gccatttccg aaaacggtca aaccaaagtc atcgaaaacg cagaaggcgc
    13801 acgcaccacg ccgtccatta tcgcttattt ggacggcggc gaaatcctcg tcggcgcgcc
    13861 tgccaaacgc caagcggtaa ccaacgccaa aaacactatt tacgccgcca aacgtttgat
    13921 cggtcacaaa tttgaagaca aagaagtcca acgcgacatc gaatctatgc ctttcgaaat
    13981 catcaaagcc gacaacggcg acgcatgggt aaaagcacaa ggcaaagagc tgtctcctcc
    14041 tcaaatttcc gcagaagtcc tgcgtaaaat gaaagaagcc gccgaagctt acttgggcga
    14101 aaaagtaacc gaagccgtga ttaccgtccc tgcctacttc aacgacagcc aacgtcaagc
    14161 caccaaagac gcaggccgta tcgccggttt ggacgtaaaa cgcatcatca acgagccgac
    14221 cgcagccgct ttggcattcg gtatggacaa aggcgacaac aaagaccgca aaatagccgt
    14281 atatgacttg ggcggcggta ccttcgatat ttccatcatc gaaatcgcca acctcgacgg
    14341 cgacaaacaa tttgaagtat tggctaccaa cggcgatact ttcttgggcg gtgaagactt
    14401 cgaccaacgc ttgattgact acatcattga cgagtttaaa aaagaacaag gcattgattt
    14461 gaaacaagac gtaatggctc tgcaacgtct gaaagaagct gccgaaaaag ccaaaatcga
    14521 attgtccagc ggccagcaaa ccgaaatcaa cctgccgtac attaccatgg acgcaaccgg
    14581 cccgaaacac ttggcaatga aaattacccg cgccaaattc gagagcctgg ttgaagacct
    14641 gattgcccgc tctatcgagc cttgccgcac cgcattgaaa gatgccggct tgagcaccgg
    14701 cgacatcgac gacgtgattt tggtcggcgg tcagtcccgt atgccgaaag tacaagaagc
    14761 cgttaaagac ttcttcggca aagaaccgcg caaagacgtg aaccctgacg aagccgttgc
    14821 cgtaggtgca gcgattcaag gcgaagtatt gagcggcggc cgcagcgacg tattgctgct
    14881 ggacgtaacc cctctgtcct tgggtatcga aaccatgggt ggtgtgatga ccaagctgat
    14941 tcaaaagaac actactattc cgactaaagc gtctcaagtg ttctctactg ccgaagacaa
    15001 ccaaagcgca gtaaccatcc acgtactgca aggcgaacgc gaacgcgctt ctgccaacaa
    15061 atctttaggt caattcaact tgggcgacat cgcacccgca ccacgcggta tgccgcaaat
    15121 tgaagttact ttcgacatcg acgccaatgg tatcttgcac gtttccgcca aagacaaagg
    15181 cactggcaaa gcggccaaca ttaccatcca aggttcttca ggtttgagcg aagaagaaat
    15241 cgaacgcatg gtgaaagatg ccgaagccaa tgccgaggaa gataaaaagc tgactgaatt
    15301 ggtcgcttcc cgcaaccaag ccgaagccct gattcactcc gtgaaaaaat ctttggcgga
    15361 ctacggcgac aaactcgacg ctgccgagaa agaaaaaatc gaagccgcgc tgaaagaagc
    15421 cgaagaagcc gtgaaaggcg acgacaaaac cgccatcgat gccaaagccg aagcactggg
    15481 tacagccagc caaaaactgg gcgaaatggt ttacgcgcaa gcgcaagccg aagcccaagc
    15541 cggcgagggc gcacaagcca atgcttctgc aaagaaagac gatgatgtcg tagatgccga
    15601 ctttgaagaa gtaaaagacg acaaaaaata attgatgccg tctgaaaaaa acgcgaacca
    15661 ttcggttcgc gtttttttca attgagataa aagacaatag catgacagag attccaaatt
    15721 catctaccgt gatattccca agcccaagtt ccaactgttg catcggttat ctggaaattt
    15781 ttcatatcat tttcttattt acttaaattt tttaataaga taataaataa taattattat
    15841 catgagctaa gaaatgaaac taaatactct cacatgggct ttgatgaccg ttttttccgt
    15901 tgcgccatct tgggcagaac aaccggcaaa tactgaagaa atacaacccg tcaaaacctt
    15961 ctccccgccc aaaccgattg caccgaccgc cgcacaaggc tatttccccg aaaaccaatt
    16021 cgaccgctcc gaccgcagcg attattactt tgttaccgaa aacatagacc aagccttccg
    16081 tccgctgaag gcaaacagca gtttttacgg caaaagcttt tacaactccg ttaccgcgca
    16141 agcactcggg gcgaaggtat acggcgtagc caacctcaat cgcaccaagg caaacggcta
    16201 caaagatggc ggtgggcgcg acactgattg gaaatacagc cgcttcaatc aggctttggt
    16261 actcggtttc gtgccgtctg aaaatcaaga ataccgcctc acttatctgc acgacgacat
    16321 caacaacgac cgccagccgc aggtcgtcaa cgacgcattg gacaccgaac gccacatctc
    16381 caaactcaac gtgcgttggg gcaatgccga tttgagcaat acggtcagcg cggaagcagg
    16441 cgtcatcaaa ctcaaacgcc atgccgacaa ttactccttg cgaccaaaca acacgccgca
    16501 gcaagtgttc gtagaacttg accgcaaagt gtatgatttc tcgctcaaac acgatgcgga
    16561 tttcggcaaa ttccacaaca cggcagccgt cagctaccgc aacgacagcc aaaacggcga
    16621 acgcaacacc cacactgcca tgtgcgattt cctcaacggc taccgtttcg ccgatgtgca
    16681 catcgaccgc tggtgcattg ccgatacctt atcttacaag tttgacgacc gacacaaact
    16741 gggcttgggc ttaagctacg aactcaacga agcggacatt cgcaaaaata cggcacagcc
    16801 tgaaaaccca ataaaacccg gtttcccctt cgcctcatca caacaaatct ggaaaaccca
    16861 ttacggctac gacttcaacg gcaaagtgcg ccgtcatgct ttgtcaggcg aactcaaata
    16921 cgatttcacg ccgtctgaaa cgcaaaaata cagcgtttcc ctcgcgcatt tggaacgcat
    16981 cggcgacaac accgaacgct tcaactcgct tgctgccatc gtgcaaaacc gtatgagcgg
    17041 catgctgatg aaccaaaatc cagccgccgc cattgcaggc aatccccctg ctgaaaactg
    17101 aaaaacacaa ccgcatcagg ctcaccgccg acagccgcaa cgactactac aacggctaca
    17161 tgaactcgct cgcaggcgcg ggctggaacg tgggcggcac gcttgtggcg gacaaagtca
    17221 aagacctgat tatttttgac cgcgcacacg gacaaagcgg cacagcttcc aaagacggcg
    17281 gcatcatcac acgcaacgtg gacgcacgat tgtttaccgc gcaggcctac gcgcgttaca
    17341 acttcaatcc gcattgggcg gcaggcatca aagccgccta caactacgga cacaacgaaa
    17401 ccgacggcag gccgccctat caaatccgtc cgtttgaagc cgccgtccaa gccgactaca
    17461 aaaactactt tgcccacggc agctacaaca tcggcgcggc aacacgcttt gtcgccaaac
    17521 aaacgcgcgg tgattttgat atggcaagcg gtctgggcat agacaaacgc gaagccgcca
    17581 aaggctttac cgttgccgac gtgtacgcag gcgtaaacat caaagacaaa tacggcttgc
    17641 gcttgggcgt gaacaacgta ttcaacaaaa aatacgtcga atacatcagc ggcgaccacg
    17701 tcctcgccct atcccccagc gtagtgtatg caccgggcag gacatattgg ttgagtttgc
    17761 atgcggcatt ttaaaaccat gccgtctgaa aaatacctgc attggcttat taagaattag
    17821 gagataaaaa atggatatga aaagacgcga tttcttaaaa atgaccgccg cgctggcagc
    17881 cgcaggcgtt tcgccttctc tgcttactgc cggtaaagag caatttaccg tgtacggcgc
    17941 accggcaatg cccagcgtta ccattgccgt aacggcgttg caaggtaaac tggcgaaaca
    18001 ggcggacgtg tcgctgaaaa tctggcgttc gcccgaccaa ctgcgcgcgg gcgtggcaag
    18061 cgggcaattt aaagtgatga tgagtccgag caatgtcggc gtaaacctgc gcaaccaagg
    18121 gcagaaagtc ggcatggtga atattttgac caacggcatc acgcagttgg tctgcaaagg
    18181 cagcgcgatt gcctcgccgc aggatttggt cggcaaaaaa atcctcgtgc cgtttaaaaa
    18241 cgatatgccc gacatcgtgc tgcaagcctt gttgaaaaaa ctgaaaatcg acgcacacaa
    18301 agtcagcatt acctacgctg ccacaccgcc cgaagcagtc ggactatttc caagtaaagg
    18361 ttaccatgcc gtcatcctgc ccgaaccgat ggcaaccgcc agcctactga aaggcaaaac
    18421 cataggtata aacgtcgtgc acggttttga tttggtaaaa gcatgggggc aggcgtttgg
    18481 caccaaaccg ctgattccga tggcaggtat catcgccaac gaagaatatt tccacgcaca
    18541 caaggcgcag ttcgacatct tccatcaaga tttgaaaaac gcgctcaact ggatattggc
    18601 caaccgccaa aacgcggcga aaatcggcaa aaactaccta cccgcccccg aacccgccct
    18661 agtcatgggc ttggacggcg cacggctgac ggtaagcaaa ggcagcgaag tgaaaaacga
    18721 gattttgaag ttttacgaaa tcctgatgca gttcaacccg gaacttttgg gcggcaagct
    18781 gccggataac gggttcttct tggcttaaag ggaaagaggc cgtctgaaaa gggtagggtg
    18841 gttgtgaata cctgatttat catttttcag acggcctatt aaggcattgt cagcggcgtg
    18901 ggtcggattc ttgaatccga catttcggct aaacggggct gtcggataca agtatccgac
    18961 ctactctttt cagacggcct atcagataat agttcgaaca gtacacaaag agccgtcgga
    19021 ttgcctgaaa tgttgggtca tgacccaacc tacgcttact ggggaaacgg gatcgaagtg
    19081 tgaggtatgt ggcggggttg tttaataatg aaattttata actatttgat agggtggtaa
    19141 tgctatgaat gaagataaat atagggaaaa tatgacatgg gatgaaattg aaaatgtagt
    19201 aggaacttta gtttctgaag aaagaatgct taaatccaat agctttttaa gtattaaaaa
    19261 tagtaagaat aggttggctt aatcacaacc ataacccaac gaaaatacta aaattccaag
    19321 ctaaggctat cctttccaaa ccatccccat caaccaaacc atgattaaaa ccgacaaaat
    19381 ccgcaaaccg cagcctgcgc tgttttacat catcgactac ctttggagcg gctttgccgg
    19441 tctgagcgtg gcgatggtgg tggtggcgtt gtgggcgtgg ggcagcgccg tgttcggcga
    19501 gtttatgctg cctgcgccgg tagaggtgtt tcaaaaatct ttggatttat tgaaacattt
    19561 tcaggaaaat gaaatcggga tttcgctgtg gcggtcggta gtgggtattt cggttgcttt
    19621 gatagcagga ttggcggcgg ggctggtggc gggcagtttt aagacggcga tggcgttgct
    19681 taagcctgtg attacgattt tgttagcaat gccgccgatt atttgggtgg tgatggcgtt
    19741 gttttggttc gggttcggca atccgagcgt gttgtttacc atcattgtgt tggttgcgcc
    19801 gctgacgttt gcgagtgcgg cggtcggaat ggcgagcgtg aacaagcagc atgaggagtt
    19861 gtttgacgct tataaattag gccgtctgaa aaaaatccgc tatctgtata tcccgcatct
    19921 gacgggctat gtgatttcca gcgtcggcgt ggcggtggcg atgggggtga aggcggtgat
    19981 catggcggaa ctcttaggcg cgagcaaagg cgtgggcgcg cggattgcgg acgcgagggc
    20041 aatgctgtag acttcgacgg taatggctta tgtggtgttg gtcatggtat ttgtgtcgct
    20101 gtttgaatac ctgattacca agcctttgta aattttgttt atgccgtgga ggagatgatg
    20161 ctctgtcttg aaaacgtgcg ttttgaaatc ctccgcgacc ccatcgtgcg cgatttcagt
    20221 ttgaacctgc aacagggcga agtgaaagcc ttgttcgggc cgagcggctg tggcaagacg
    20281 acggttttac ggctgattgc gggcttggaa acgccgaaat cgggcacgat acgtaatact
    20341 ttccacaaaa cgggttttct gtttcaggaa aaccgcctgc cggaaaacct gaccgcgatg
    20401 cagaatatcg cgatttttat ggacaaccca gacgaaggcg aaatcgtcgc gctggcggcg
    20461 aaagtcgggc tgactgcggg cgatttgaac aaatatccga ccgaattgtc cggcggcatg
    20521 gcgaaacggg tggcgttttt gcgcctgctg ctgtgcggct gcgacctagc cttgctggac
    20581 gaaccgtttg tcggtttgga ccgcgatttg cgcgatattt tggttgctat gctggtggaa
    20641 aaaatcgagc ggcagggcat ggcgtgtata ctggtaacgc acgaccgctt cgaagccgcg
    20701 cgcctgagcc atgaaattat gctgctttcc gctaagggca tgaacgtgca aaacgtgata
    20761 accctgccca cgccgctgtc cgaacgcgat tcggcttttg aagaagtcgt ggtggcaagg
    20821 gagtttcagg ggattcatta ttatgagtga tgaggggatt tgagtgtctg acaaacctta
    20881 cggttcgttg ccctctccct aaccctctcc cacggggaga gggttagcaa gcctcaggct
    20941 tgcctcttta gcgaaagata cgaatctgtt atccgaacgc gattcggttt ttgaaaaagc
    21001 cgtggtgcca agggggcagg ggattcatta tgaggtgctt tatgttttcg actgtgatta
    21061 ctgctgctgt tttatatatt gctacagcag tagatttgtt ggtaatacta ttaatatttt
    21121 ttgctagagc aaatactaga aaagaatatc gagatattta tatcggacaa tatttaggtt
    21181 ctgtaatttt aatattagtt agtttatttc tagcttttgt tttgaattat gttccggaaa
    21241 aatgggtgtt gggtttatta ggtttaatac cgatttactt aggtattaaa gttgctattt
    21301 acgacgattg tgagggcgaa aaaagagcta aaaaagaatt ggatgaaaaa gggttgtcaa
    21361 aattagtcgg tattgttgct ttggttacag ttgctagttg tggtgcagat aatattggac
    21421 tttttgttcc ttactttgtg actttagatc ttgtcgactt attagttact cttcttgtat
    21481 ttttaatatt gatttttgtt ttagtatata cagcacaaag attggctaat atttcaggtg
    21541 ttggtgaaat tgtagagaag tttagtcgtt ggataatggc tgttatttat attggtttag
    21601 ggttatttat tattattgaa aataatacaa ttcgaacaat aatatcaata atatgaatga
    21661 caagggcatt tcagtatctg acaaaccttg cggttcgttt ccctctccct aaccctctcc
    21721 cacaggagag gggatcaggt tgctgaaaat cagagggcga aggattgggg atcaaatgga
    21781 aggtaaaacc tgaaaatgtt cgggtttgac agcccgattc cctctccccg tgggagaggg
    21841 ctagggagag ggcaagcaag ccgcaggctt gcctctttgg cgaaaaatac aaccctgtcc
    21901 gcccaataaa tccatacccg aaagcatctt catacagaat caagccaagc gatgaataaa
    21961 ttttttaccc accctatgcg cccgtttttc gtcggtgcgg cagtacttgc catactcggt
    22021 gcgttggtgt tttttcatca accccggcgc tatcatcctg caccgccaaa ttttcttgga
    22081 acttatgctg ccggctgcat acggcggttt tttgactacc gctttgttgg accggacggg
    22141 tttttcaggc aacctgaaac ctgccgctac tttgatggcg gtgttgttgc ttgttgcggc
    22201 tgttttattg ccgtttttac cgcaacttgc cgcatttttc gtcgccgcct attggctggt
    22261 gttgctgctg ttctgcgcct ggctgatttg gctcgaccgc aacaccgaca acttcgctct
    22321 gttgatgtta cttgccgcat ttaccgtttt tcagacggcc tatgccgtca gcggcgattt
    22381 gaacttactg cgcgcgcaag tgcatttgaa tatggcggcg gtcatgttcg tatccgtccg
    22441 cgtcagcgtc cttttgggca cggaaaccct gaaagaatgc cgtctgaaag accccgtatt
    22501 catccccaac gttatctata aaaacatcgc catcaccctg ctgctgcacg ccgccgccga
    22561 actttggctg cccgcgcaaa ccgccggttt tactgcgctt gccgtcggct tcatcctgct
    22621 cgccaagctg cgcgaactgc accatcacga actcttacgc aaacactacg tccgcactta
    22681 ttacctgctc cagctctttg ccgccgcagg ttatctgtgg acaggcgcgg cgaaactgca
    22741 aaacctgccc gcctccgcgc ccctgcacct gattaccctc ggcggcatga cgggtggcgt
    22801 gatgatggtg tggctgactg ccggactgtg gcacagcggc tttaccaaac tcgactaccc
    22861 gaaactctgc cgcatcgccg tctccatcct tttcgcctcc gccgtttcgc gcgctgtttt
    22921 aatgaacgtg aatccgatat tcttcatcac cgttcccgag attctgaccg ccgccgtgtt
    22981 catgctttac ctgctgacgt tcgtaccgat ttttcgagcg aacgcgttta cagacgatcc
    23041 ggaataaaaa tgccgtctga aacggttttc agacggcatc ggattgccaa tgatcaagcc
    23101 tcctgcggat cgacatccac cgaccatcgg attttgccgt cgcggttctg ctgcaacacc
    23161 tgcacccaca aactcacggc gcggtgcaaa tcttgtcggg atgttgattc gaggaaaact
    23221 tgcgcacgtt cgcgttcggc aaggcgcacc atcagcatcg gggcggcacc gaaccgggag
    23281 acgctttcgg gcaagagcgg ggcgagggtt tctttggcgg cgttgagaaa ttccatcgca
    23341 tcggcaacac gcggcgcatc ggcacggacg gccgtctgaa aaccgaaagg cggcatggcg
    23401 aacatttgcc gttcgttcaa ttcgttttcg gcaaacacgg cgtagtcctg cgctttgacg
    23461 gcggcgaaga cagggtgttc gggcagttgg gtctgtatca gcaccttgcc gggtttgtcg
    23521 gcgcgccccg ccctgccgga cacttgcatc agctcggcaa acagcctttc cggcgcacgg
    23581 aaatccgcgc tgtacaggct gccgtcggcg ttcaacacga taacgaggtt gagccgcgcg
    23641 aaatcatggc ctttggcaag catctgcgtg ccgaccagaa tgtcgatttc gtcgttggcg
    23701 atgcggcggt acaaatctgc ccagtcgttt ttgtgcgccg tactgtccct atcgacgcgg
    23761 acgacggctg ccttgggcag gaaggcgcgc agggtttctt cgacgcgctg cgtaccgtgt
    23821 ccgacggcgg tcaggtcttg gttgccgcag tcgggacatt tgaacgggat gggttcgcgg
    23881 tggtcgcagt ggtggcagcg cagttggcgg gcgcgttggt gcagcaccat tttggcggag
    23941 cagttcgggc agccgaaggt atggccgcag tcgccgcaaa acagcgcagg cgcgaagccg
    24001 cgccggttaa ggtacaccag cgacataccg cccgcttcaa agttctgttt caaaagctgc
    24061 aaggcttgcg gtgagaagcc gttatcgagt ttcagacgac ctatgttgag gatgtccact
    24121 tgtggcagtt gcgcggaagc atgggcgcgt tcggtcagtt gcagcaggcg gtacgcgccg
    24181 ctttgcgcct tgtgccaact ctccaagctg ggcgtggcac tgcccaacac gacgggacag
    24241 ccgccctgct tcgcccgcca caccgccaaa tcgcgggcgt ggtagcgcaa ttcgttgtct
    24301 tgtttgaacg agccgtcgtg ttcctcatcg accacaatca gcccgacatc aggcaaaggc
    24361 gtaaacaccg ccagccgcgt gccgatcacc agcttcgcct gccccaacat cgcgcgcaga
    24421 taatcctgcg tgcgcctgcc tgccgccatc cggctgtgca acacggcggt cggcacgtcg
    24481 gcaaaacggt tttccacccg ctttaaaagc tgcggcgtga ggttgatttc gggcaacaga
    24541 aacaacacct gtcgcccctg cgccaacact ttcgccatcg catcgaaata cacctcggtc
    24601 ttgccgctgc cggtaatgcc gtacagcaga aacgaccgga agcgtcccaa tgccgtctga
    24661 atttcatcgg aagccttctg ctggccggta tttaatacaa attcagagtg cgaagcctgc
    24721 ccgcggtacg gccttaaaac aggtttcgcc gcttccgtcg tttcaatcca gccctgctcc
    24781 gcccaatcct caatcaattt cgccgcctgc gcgtttgcct gtttcaacgc cgccatcgtc
    24841 atttcgcccg acagcagcgc gtcccacaaa gccgcttttt tgttgaaccg agccggcggc
    24901 ggcgtttgcg ccctgcccgc ttcgttcaaa gcataaaaca acggcggctg cggcatttcc
    24961 accgcgcgcg tttccttcaa accctgcggc aatgcggcaa atacagcctg cccagtcgga
    25021 tagtggtaat aacgcgacgt aaacgccaac aaatcacgcc agctttgaga cagcggcttt
    25081 tcttccacaa agaccgtctg aacgctcaaa atccgcgccg catccatatc gggcgcaata
    25141 tccgtttccc acaccatccc gacaacagtt ttattgcgaa aaggcacaag cacccgcgtt
    25201 cccggaggaa gcggctcgga atgcgaataa gtcaaaaggc cgtctgaaag cggcacgttt
    25261 acagcgatgc gatggtagat catacaggca ggcaaatcat ttttttgcgt attttaacag
    25321 ccggaagcta ttttcagacg ataaattgag cctcggcgca atgcaggcgc aacagaggtt
    25381 tgatacaatg ccacttttcc aacacgctca atcatctttg atatgaaaac caagttaatc
    25441 aaaatcttga ccccctttac cgtcctgccg ctgctggctt gcgggcaaac gcccgtttcc
    25501 aatgccaacg ccgaatccgc cgtcaaagcc gaatccgccg gcaaatccgt tgccgcttct
    25561 ttgaaagcgc gtttggaaaa aacctattcc gcccaagatt tgaaagtgtt gagcgtcagc
    25621 gaaacaccgg tcaaaggcat ttacgaagtc gtcgtcagcg gcaggcagat tatctacacc
    25681 gatgccgaag gcggctatat gttcgtcggc gaactcatca acatcgacac gcgcaaaaac
    25741 ctgaccgaag aacgcgccgc cgatttgaac aaaatcgact tcgcctccct gcctttggac
    25801 aaagccatca aagaagtacg cggcaacggc aagctgaaag tcgccgtctt ctccgacccc
    25861 gattgtccgt tctgcaaacg cttggaacat gagtttgaaa aaatgaccga cgtgacggtt
    25921 tacagcttta tgatgcccat tgccggcctg cacccagatg ccgcgcgcaa ggcgcaaatc
    25981 ttatggtgtc agcccgaccg tgccaaagcg tggacggatt ggatgcgtaa aggcaaattc
    26041 ccggtcggcg gcagcatctg cgacaatccc gtcgcggaaa ccacttcctt gggcgaacag
    26101 ttcggcttca acggcacgcc gaccctcgtc ttccccaacg ggcgcaccca aagcggttac
    26161 agcccgatgc cccaactgga ggaaatcatc cgcaaaaacc agcagtaaac ccgcaatgca
    26221 aaaggcttgg aaccatgttc caagcctttt tcaaatgccg tctgaagcct gtcgcggcag
    26281 cttcagacgg catctttgtt caaacctcaa tcctgcgcca atgcgtctat cggattgagt
    26341 ttggctgcct tattggcggg cataaagccg aacgcgatgc cgattccggt cgaacaggcg
    26401 accgccccga taacggatgc cgccgaaatg tccatcggga aatcggttac aaaatgattg
    26461 aacacgaggc tgacggcggc ggacaaacct acgccgacca agcctccgat gatgcagatt
    26521 aacaccgcct caatcaaaaa ctgctgcaaa atattgccgc gccgcgcgcc gattgccatc
    26581 cgtatgccga tttctttggt gcgctcggta acggacacca gcatgatgtt catcacgccg
    26641 atgccgccga ctaccaatga aatcagggcg atggaggaaa tcagcagctt catcgtaccg
    26701 gtggtgcttt cgaccatctg cctgatgctg tcgctgttgt tcataaagaa gtcttccgtg
    26761 ccgtgccgtg ctttgagcag ctcggccagc cctttttcgg caacccgggt attggcattg
    26821 tctttgattt tgacggtgat ggagttggtg tggctctcgc ctgtgatttg gtgcatcacc
    26881 gtcgtatagg gcgaccaaag catcagcacg tcggaattgc cgaaagcgtt ttcgtctttt
    26941 ttcatcacgc cgatgacggt caaggggcgt ttcctgaaca aaatggtttt acccaacgga
    27001 tccgagtccg caaagagttt gtctttgaca ttttggtcga tgacgacgac ttgcgcgtct
    27061 tctttcacat cgttctcatc aaacagccgc cccgtttcca gcttcagccc gcgcacgtcg
    27121 aaatattgtt cgcccacgcc gtacaaagaa gcggtcaggt cggtattgcg gtaggtcagc
    27181 gtgccgccgc tcgaagtcat gggcgtggcg gaggcaacgt agctttgttt ggcgatgatt
    27241 tttgcgtcgt ctatggtcag ggttttgatt ttgccgctgc gcctgtcgcc gaagccgcgc
    27301 ccggggaaga tgctgatggt gttcgtcccc atcgaactga tgtcttcgag gatttttttc
    27361 tgcgaaccgt tgcccaatgc gacgacggag acaaccgaag cgataccgat gatgatgccg
    27421 agcatggtca gaagcgaacg cattttgtgc gccaatactg cttgcaccga cattctgaag
    27481 gcttcgacaa actggtcgta ataaaacgac cacgaggctt tttcctgaat cctcccgaca
    27541 ttgcttgcgg ggatttcggg atttttcgag gtgtcggaaa tgatttcgcc gtcccggatt
    27601 tcgatgacgc ggttggcgtt ggcggcaatg ccggggtcgt gcgtgaccat aatgacggta
    27661 tgcccggctt cgtgcagcct gcggatgatt tccatcacat ttttgccgct ggcggtatcg
    27721 agtgcgccgg tcggctcgtc ggcgaagatg atttctccgc cgttcatcag ggcgcgggcg
    27781 atggagacgc gctgctgctg tccgcccgag agttcgccgg gcttgttgcc ctctttactt
    27841 gccaaaccca aatcctgcaa gagtttgtcc gcccgcgcgg aacgctcttt gccgcccata
    27901 cccatataca cggctggcag cgcgacgttg tcccttgcgg tcagcgagct taagaggttg
    27961 tagcgttgga agatgaaacc gaagcgttcg cggcgcaatg ccgccagttc gtcaggctgc
    28021 attttggcag tttcgatgcc gtcgattcgg tacgaaccgg aaccggcggt atccaaacaa
    28081 ccgagtatgt tcatcagcgt ggacttgccc gaaccggact gcccgatgat ggcgacaaaa
    28141 tcacccttct ctatcgacag gctgatgtct ttcaaaatat ggacgcggtt ctcgccgctg
    28201 ccgaagcagc ggttgatgtt tttacattcg atcaagctca taatattccg taaatcaata
    28261 acaaatgccg tctgaaaccg tttccgtgtt cagacggcat attcgtttat cggcgcggcg
    28321 ggccgcctag ggcgcgttcg ccgctttcct gctgctcggc ggcggttatt tcggagatga
    28381 ccactttgtc cccctctttc aacccgcttt tcacttcggt attcatactg tctctcatac
    28441 cggtccggat ttcgcgttcc gccgccttgc cgtccgcacc caacacgcgt acgaacgcct
    28501 tgccgccgcg atttttcacg gtcagcgacg gaataagcaa cacatttttc acaccgtcga
    28561 tttcaaccgt attctgcgtc gtcatccccg tggcgagttt gccgtccgga ttcggcacaa
    28621 acgaacgggc ataataatag accgcattgg aagccgtatc cgtactgctg ttgtagccgc
    28681 ccgacgacat cgtggtcagc ccggggtcga cgctgtcgag cttcgcctta atcggcgtat
    28741 ccggttcgga caaaatcgta aacgaaatat cctgccccgc cttcaccttg gtaatatcgc
    28801 cctcggcaat ctgcattttg ttcaacatca tatccagatt cgccaattgg acaatcgtcg
    28861 gcgtagactg cgccgcgttc acagtctgcc cctcttccac gggaatcgcc accaccgtgc
    28921 cgtccatcgt cgcggtaatg cgcgtgtagc ccaaatccga ctcggcggta ttgatggaaa
    28981 ttttgctctg tctgattaaa gccttcaact cggcaacatt ggctttggcg gcggcaagcg
    29041 catcctgcgc gctttccaaa tcttctttag aggtcgcatc atccttccac aacgccgcct
    29101 gacgcttata tttcttctcc gcgctgccca atgcaatctg tgccgacacc agcttcgcct
    29161 gatacgtttc caatttggat ttttccatat cgagcgtgtt ggtctgcgtg gtcgaattga
    29221 tttccgcaat caaatcgccc tttttgacct gttgcccgag tttgacataa agctttttaa
    29281 tctgccccga agcctgcgcg ccgaccgata ccaggttgga cggcgaaatc tcgcccgtcg
    29341 cggaaaccgt ccggctgatg ccgccgcgcc tgaccgtttc cgtaatataa gaagcctgcg
    29401 gctcgggctt cagataatgc catccgcccc aaaccgctgc cgccgcgact gccgcaacag
    29461 ccgaccattt catcattttc gccatatttt caattctctg tatgcgtcaa tcccataaac
    29521 cggaagcgga ttctaccgca agtccctgcc ccatccaagg cggcaaaccg ccgacaataa
    29581 aaataaccat ttgttttaaa tggcttatac aatctgcttt cccgaaaatt tcaatccgtt
    29641 tacatattcc gccatttccg atacggcgtt ataatgccgc ccacaaacaa ccaaatggaa
    29701 acacattgct atgaaaaacg ttcaaaaagg ctttacgctg ctcgagctga tgattgccgt
    29761 cgccatcctc ggcatcctga cgctcatcgc ctacccttcc tataaaacct acatccggcg
    29821 cgcccgcctg tcggaggtca aatccaccct gctggtgaac gcgcaaaact tggagcgtta
    29881 ctaccgccaa aaagggacgt ttgaaaaata cgactcaacc aaactgaaac aaaacaaata
    29941 tttcaagatt accttaatca aagccagccc cgaccacttc acccttcagg ccgctcccga
    30001 tacggcgacc aacgaaggcg aaacctgcac cgtcacgctc aacgacggcg gtaccattgc
    30061 cgcttccggt acaaaccaat cctgccccgg cttcgactga ttggctgttg cagtcggcat
    30121 tgtgcctaaa ccatctataa aaatgccgtc tgaacgctgt tcagacggca tttttgacat
    30181 cccgatacgc ctttcaaacg gcttccgcca tttccaacgc ctgcttgatg tctacggcga
    30241 cgacgcgcga cacgcctttt tcctgcatgg ttacgccgac cagctgctcc gccatttcca
    30301 tggtcaggcg gttgtgggag atgtagagga actgggtttg caccgacatt tctttgacca
    30361 gcttgcagaa acgcgaggtg ttggcatcgt ccagcggggc atcgacttcg tccagaaggc
    30421 agaagggggc ggggttgagg ctgaacaggg cgaacacgag gctcatggcg gtgagggctt
    30481 tttcgccgcc ggagaggagg tggatggtgc tgtttttctt gccgggcgga cgcgccatga
    30541 tggacacgcc ggcggtcagg aggtcgtcgc ctatcatttt gagggtggct tcgccgccgc
    30601 cgaacagggt cgggaagaag gtttggactt tgccgttgac ggcgtcgaaa gtttctttga
    30661 aacgcgcttt ggttttgtcg tcgatttggg cgatggcttc ttccaaaagc gcgatggcgg
    30721 cctgcacgtc ttcgctctgg ctgcggtagt agccgtcgcg ttcgcgcgct tcttcgagtt
    30781 cttgcagggc ggcgaggttg acggcgccga gggcttcgat ttgccgggta aggctgccga
    30841 tgctgctgtt caatactttc gccgattctt tcgccaacgc ttcgagcacg tccagatcgg
    30901 cagcgcgttc ggtcaggttt tgatggtagc gtttggcgtt gatcagggcc tcctgctgct
    30961 gcaacagggc ggtttgggtg gcggcctgaa gctgcggcag cttggtttgc agggtttgta
    31021 cgcgggcgta ttgctccctg ccctgttcct gaatctgcgc gagtttttcc tgcacggcga
    31081 tgtattcttc gtccagcgtg tgtacggttt cggtcaactc gtcgagcttg atgtgctgct
    31141 cgtcgttttg gaactcggtt tcataggcga gggcaagctc ttgccggcgt tcctgccagt
    31201 ccagggtttg ctgttcgagc cgggcgattt gctgccggta gttttgtttt tgctggttga
    31261 gtttgtggac ggcgacttcg gcaagcccgt attggcggtt ggcttccaac agggcaagct
    31321 gcgcctgttt cagacggcct tgctgctctt ggcggctgtg cgcggtggtt tgctgctggt
    31381 gttcgagttc ggcggcggct tcctgcaaag tagcgatgtc gtctgaaagc ccgtcggacg
    31441 tgtgttgcaa cacggtctgc tcttccgcca actgtgccag ttcgcgctcg atgtgttcgc
    31501 ggcggatttg cccttggttg gtgcgcgcca gaagttcggc ggtgcgttgc tgcgcttggc
    31561 tgtattggcg cgtgtgttgc tgttgctgct gcatcagatt tttgtgctgc acttcggacg
    31621 agcgcacggc agcctccgcc tgtttgaacg cggcttcggc ggcggaaagt ccgggggcga
    31681 ggttttccag ttcggacgcg atgccgtcga ggcgcgcttt ttgggcaatc aggctttcct
    31741 gcgcgggctt ggcatagagc aggacgctga ctttatcgac ctgatggcct tcgggtgtga
    31801 gccagatttg atgcgtgccc aaatcgcttt gatgcgcgag ggcgtagctc aaatcgggcg
    31861 cgcacaatac gccgtcgagc cagtggtgca atgccgtctg aaacggcggc tgcgcttgga
    31921 tttggttcag caatgcctgt acgggcaggg attttttgat gccgccggag aggccgtctg
    31981 aaagccatgc cgcctgcccc tgcggcaaag gtgcgggcgg tacgaaacta ttcggcacgc
    32041 tacgggcgtg caggcgttcg gcaaggatga cggacagggc gtgctgccac tcggcgggcg
    32101 cggtgatgtg ttgccacagt tgcggcgcgg cggcgtggtc ggttgcctgc caaaagtcgg
    32161 cggcttcctg ctgttgcgac aggatttgcg acaacgcctg ctgctgcgcc tgcaaggtaa
    32221 tgtgttgctg cttcaggctt tggaagcggt ttgaggccgt ctgaaacgcc tcgcgggcgg
    32281 cgtgcaaggc ttcttcggcg gcaatgattt gttcttcgta atgctcttgc cggctttgca
    32341 acaaggcggc ggcttcctga gcggcggcgg tttcggcctc gtcgggcagg ttcagggctt
    32401 ggttttcctg cttcagacgg cctttgcgct cttcgtgctt ggcaacggtt tgttcggcat
    32461 gggcaagctg ctgctgcttc aacgccagtt cgcggcggat gcggtttgcc tcgtcctgct
    32521 gggtttggaa ggcagcgttg agcgtggctt ggacttcttc caattcgggc agacgctctt
    32581 cgtgttcggc aacctgcatc gcccattccg ccaattcggt ttgcttttct tcgacctgca
    32641 actcgttttc ctcaagctgc acgcggattt gctgctgctc ctgccggatg cgttgcaact
    32701 gcgcctgcgc cgcctgcttg tcgcgttcga tgcgttggtg caggttttgc cggtggcgga
    32761 tttgttcttc caaacgggca atctgctcgc gcaacacgcc gcgtttgttg ctcaattcgt
    32821 gtactgcctg ctgctgcgac tgttcggcag tctgcaaggc gtgtacttcg ttatttaacg
    32881 cctgaacctg tgcggcggtt tcatcctgct gcgcctgcaa agattgatgc tgcgcggtcg
    32941 ccttgtcggc ggcggcaagc gattgctgcc attgggcgta atcgagcaaa tcctgttgtt
    33001 gattcaactg cgcggtcagg gatttgtagc gttcggcggt ttccgcctgt ttttccagct
    33061 tttccacctg acgcgccaac tcgttctgca aatcgcccaa acgctgcaaa tgctcgcgcg
    33121 tgtctttcag acggccttcc gtctccttgc ggcgttcctt atatttggac acgcccgccg
    33181 cctcctcgat ataggcgcgc aactcctccg gccgcgcttc gatgatgcgc gaaatcatcc
    33241 cctgctcaat gacggcataa ccgcgcgcgc ccacgcccgt acccaagaac aaatcggtaa
    33301 tatcgcggcg gcgcacggtc tgattgttga tgaaataagt cgattccccc tgacgcgtca
    33361 gctgccgctt gatgctcacc tcggcatact gcccccacgc gccctgcaaa ctgtggtcgc
    33421 tgttgtcaaa taccagctcc accgaagccc tcggcgcagg acggcgcgtc gccgcaccgt
    33481 taaaaatcac gtcctgcata ctctcgccgc gaagctgctt cgccgaagcc tcgcccaaca
    33541 cccagcgcac cgcgtcaatc acattcgact tgccgcagcc gttcggcccg ataaccgcga
    33601 caagctgccc cggaacatga atcgtggtcg ggtcggtaaa agatttgaag ccggagagtt
    33661 tgatgtgggt caggcgcata atggtcggaa aaaataaaaa agaagcgtat tttaacggaa
    33721 atcccgccgc accaccccat atcttgccgg caaaacctta ccgcatcccg ccccggtgcg
    33781 gcaggatttt gcacatcccg gccatccgta ttaaaatctt tgcgtttccc acactttcct
    33841 gacaaatata cggaggagga ttccatgaaa acaaaaacag tcaagcaagc aagcaagcaa
    33901 gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaaaatagt ctaacccccc
    33961 tttctttaat cgctctactt aatttccact tctttttatc ctgttcgatc ttttgcggct
    34021 gcacctcaag aaataaacta ttcgttatca ggcggtaaag tttacgcaga taagttcagt
    34081 atcgccattg gggttgaggc aaaagctcag ggggataata ccaaacttag caacctcaat
    34141 cggcacatct gccgaagcaa atgcacccgg ggcattggct ttaaggggaa gttctgaagc
    34201 atcgaaaaaa tttagtatag cagaaggcta tctggcatct agtgatggct atggtgcaat
    34261 agcgattggt tctgccgcca agataaagca attggaaaaa ggcacaataa atcatattgt
    34321 gggaaacgat aataaaggtc tttatgtcga tgcagacggc aatgtaacaa agataaccgt
    34381 aaggactgag tcggaaaaag atattctctc aagatacggt caaacttacg gtgcggtggc
    34441 actagggttt agatcttctt cacataatct ttttgccagt tcatttggag cgttttctac
    34501 agccacagct attgaaagct tggcagtcgg cgacagcagc caatcaacgg gctaccgcag
    34561 tgctactttt ggcagtcaca gcagggcttt ggcagaagaa agtttggcat taggttatga
    34621 aactcgggca aatgcttatg gttccgttgc tttaggtgca gaatctgtgg cgaatgaaga
    34681 aaataccgta tcagtgggtt ccgatacatt gaaacggaaa atcgttaatg tcgccgatgg
    34741 cacggaagat ttatgatgca gtaaatgtcc gccagttgaa ccgcttaagc aaacgtacaa
    34801 accgcgtcgg cgcaagtgct gcggcgttgg cttcgttaaa acctgcacaa ttaggcaaaa
    34861 acgacaaatt cgccttttct ttgggctttg gaagctataa aaatgcccaa gcggtggcaa
    34921 tgggggcggt gtttaagccc gctgaaaatg tgttgcttaa tgtggcgggc agttttgccg
    34981 gaccggaccg ggctttcggc gcgggggttt cttggaaatt cggcggcaaa ccgacacctg
    35041 cggttgccgc acaaaacgcg gcgcatcctg caaaagtttt gcaattgcgg caggaagtgg
    35101 cggcgttgcg cgcccggcag gcagaaaccg accgcaaact gcacaaacag gccgaaatgg
    35161 aaaacgagct gcaacagttg cgccgcgcgt tgtccgaatt gaaaaaacat tgaagcaata
    35221 aacgtaaggc gggacggcgg ctctgccttt ttccgatgcc gcacccgcgc gatgccgtct
    35281 gaagccttca gacggcattt ttaacgcgcc cgaaccccgg ttccgccaat gcccgatgct
    35341 tggcgcggca tttgccgtat tgccccgccg ccgggcgcgt tataatccgc cacgacatcc
    35401 gcccgatttg gaaacctgcc tatgcgaccc tcctatttca tttccgacct gcatttgagc
    35461 gaaaagcacc ccgaactgac cgaactgctg ctgcgttttt tacgttctgc cgccgccggg
    35521 caggcgcggg cggtttatat tttgggcgat ttgtttgatt tttgggtggg cgatgacgaa
    35581 gtttccgagt tgaatacttc ggttgcgcgg gaaatcagga aattgtccga caaaggcgtt
    35641 gccgtgttct ttgtcagggg caaccgcgat ttcctaatcg gtcgggattt ttgccggcag
    35701 gcgggtatga cgctgctgcc ggattactcg gttttggact tgttcggcag caacaccctg
    35761 atttgccacg gcgacacatt gtgcaccgac gacaaggcat acctgcgttt ccgccgcatc
    35821 gtgcattgca ggcggctgca aaaactgttc ctgatgctgc ccctgaagtg gcgcacgcgc
    35881 cttgccgcca aaatcaggcg tgtcagcaaa atggaaaaac aggtcaagcc cgccgatatt
    35941 atggatgtca atgccgcctt taccgcgcgg caggttcgcg cctttaacgc ggaaaggctg
    36001 atacacggac acacccaccg cgagcatatc catcacgaaa acggctttac ccgcatcgtt
    36061 ttgggcgact ggcataacga ctatgcttca atcctccgcg tggacgggga cggcgcggta
    36121 ttcgtgccgc cggaagaatg ctgaaaatgc cgtctgaagc ctttcgggcg gctttttcta
    36181 cacgcccctt tcccacactc cgtccgccgc atcatcacca tccttaaccc gcattaatag
    36241 ccattatcga taaaatgctt gaccgattac ataagattac gtaaagtgtg caaacgcata
    36301 ataattggtc ttaccaattt atcttttaac caaaatatcg ggtgtgcggg atttgaacgt
    36361 ctttccaaac ctcgacgcgc cttatgatgc aaatgcctaa aaaggattcg ttatggcact
    36421 tttcctcagc atattcccca tcgtcctgct gatttggctg atggtgaaaa aaaacagtat
    36481 gccttcctac gtcgcactgc cgattaccgc agtgctgatt tacgccatca aacttttcta
    36541 cttcggcgat gcgggaatgc tgctcaacgc caccgccgct tccggcctcg tcaaaacgct
    36601 cacgccgatt accgtgattt tcggcgcgat tatgttcaac cgtatgatgg aaaccacggg
    36661 ctgcatcgat gtcatccgca aatggctggc gaccatcagc ccgaaccccg tagcgcaact
    36721 gatgattatc ggctgggctt ttgcctttat gattgaaggc gcatccggct tcggcacgcc
    36781 tgccgcgatt gccgcgccga ttctgatgag cttgggcttc aatccgttga aagtggcgat
    36841 tttcactttg gtgatgaatt ccgtgccggt atcgttcggc gcggtcggta cgccgacctg
    36901 gttcggtttc gcaccgctga acctgagtgc cgaagacatc ctcgccatcg gcaggcagac
    36961 cggcgtgatg cacttcttcg caggtttcgt catccctgtc atcggtttgg gcttcatcgt
    37021 gccttggtct gaaatccgca aaaacttggg cttcgtcgcc attgccgtct tctcctgcac
    37081 cattccttat gccgcattgg cgatggtcaa cgaagaattc ccgtcgctcg tcgccggcgc
    37141 aatcggcctg atggtgtccg tattcgccgc caaccaaggc tggggcttga gcaaagacca
    37201 cgccaaagac ccgaatgccg aaaaagtgcc gttcgcccaa gtcgccaaag cactcgcccc
    37261 tttgggtatg ctgatcggca tgctggtggt tacccgcatc aaacagctcg gaatcaaagg
    37321 cattttgacc agcaaagaag aatggttcag cttccaactg ccgtttgatt tgtccaaaat
    37381 taccgtcagc gactccctga cgattacctt cggcaatatt ttcggacaag atgtcagcgc
    37441 gtcttaccaa acgctgtacg tcccggcttg gattccgttc gtgctgaccg tttggatttg
    37501 catcctgctg tataaaacca aattcaaaga tgcctggacg atttatgccg taaccttcaa
    37561 tcaaaccaaa aaaccgctgc ttgccctgat gggtgcgctg attatggttc agctgatgct
    37621 ggtcggcggc gacaattcga tggtgaaaat catcggtaag gaatttgccg caatggcggg
    37681 cgaacactgg gtttattttt cgccgtatct gggcgcgatc ggtgcgttct tctccggctc
    37741 caacaccgtg tccaacctga ccttcggtcc gattcagcag caaatcgccc ttgataccgg
    37801 cctgtccgtg accctgattc tggcgttgca gtccgtcggc ggcgcgatgg gcaatatggt
    37861 gtgcctcaac aacatcatcg ccgtatgtac cgtattggac gtgaaaaatt ccgaaggtgc
    37921 gattatcaag aaaaccgtta tcccgatggc gatttacggc gtgattgccg tcgtcgcggc
    37981 aatgattttc ttcctctagg cgaaacgccg ccatccatag cggcagcacg cggggaacgg
    38041 aaaccgccgc tcgtccgggc ggcgcgtcgg gcagaatacc cgtgccggaa cagcccgccc
    38101 cgccaaacaa atgccgtctg aaaccggaaa aggcttcaga cggcattttt atcgccgttt
    38161 gcattcaggc gtagtaatga tgcggcatct tgccgtcaag gcggtagcgc gtgccgcaat
    38221 acgggcaggc aacgctgccc gattctcctt cgcccaaagg taaaaacacc ctcggatgcc
    38281 cgttccactg ctcgttgtcc ggtcccgagc aatacagcgg cagattttcc ggcaacacgg
    38341 aaatttcctg cggattcaga ttgtccattt gattttcctt tgcgtggttg gcgtgcgccc
    38401 attttacgcc atcggcaggc taaaggatat tttcggcgca aagccgcaat ccgctataat
    38461 ccccactttt cagacggcat accatgactg cgcttaccct tcctgaagac atccgccaac
    38521 aagaaccatc cgttctgctc tataccctcg tttccgccta cctcgaacac accgcccaaa
    38581 ccggcgacga atccctctcc tgcctgtccg acgaccagca cacgctgacc gcattctgct
    38641 acctcgacag ccaagtcgaa gaaggcggct tcgtacagct tatcgcctcc ggctacggcg
    38701 aatacatttt ccgcaacccg cttgccgaca gcctgcgccg ctggaaaatc aaagccgtgc
    38761 cgaaagtctt ggacaaagcc aaagccctct acgaacaaca cggcgaaacc atcgaaacgc
    38821 tcgccgacgg aggcgcagac atcgaatccc tgcgcaaaca gttccccgac tttgaagaat
    38881 gggacggcgc atactacgaa gccgccgaac aagacctgcc cctgcttgca gaacacatcc
    38941 tgtcaaaccg ggcggcattc gcccatatcg ggcaggcgtg attgcgtctg tttccagccc
    39001 gtgtaaaaca gcgtaaaatc ggcaaacccg aattatcttc cgtccccatc aaggagcaag
    39061 ctatgttctt caagcacatc gaagccgccc ccgccgatcc gattctcggt ttgggcgaag
    39121 cgttcaaagc cgaaacccgc cccgaaaaag tcaacctcgg catcggcgtg tacaaagacg
    39181 catccggcgc gacacccatt gtcaaagccg tcaaagaggc tgaaaaacgc ctgttggaaa
    39241 gcgaaaccac caaaaattac ctgaccatcg acggcgttgc cgactacaac gagcaaaccc
    39301 aaatcctgct gttcggcaaa gaccacgaaa tcatcgccag ccgccgcgcc aaaacagcgc
    39361 aaagccttgg cggtacaggc gcattgcgta ttgcggccga atttgccaaa cgccagttga
    39421 acgcgcaaac catctggatt tccaatccga cctggccaaa ccacaacgcc attgccaaag
    39481 cggtcggtat ccaagacaaa ccttatcgct actatgatgc cgccaaacac tgcttggatt
    39541 gggacggcat gattgaagat ttgaaccaag cgcaaaaagg cgacatcgtc ctgctgcacg
    39601 gctgctgcca caaccctacc ggtatcgacc ctacgcccga acaatgggaa actttagcaa
    39661 aactttctgc cgaaaaaggc tggctgccgc tgtttgactt tgcctaccaa ggctttggca
    39721 acggtttgga agaagatgcc tatggcttgc gcgtgttctt gaaacacaat acagaattgc
    39781 tgattgccag ctcttattcc aaaaacttcg gcatgtacaa cgagcgcgtc ggcgcgttca
    39841 ctttggtggc cgaagatgaa gaaacagcag cccgcgccca cagccaagtc aaaaccatca
    39901 tccgtacctt gtattccaac ccggcttcac atggtgcgaa caccattgca ctggtgttga
    39961 aaaatgatga tttgaaagca caatggattg ccgaactcga tgaaatgcgc ggccgcatca
    40021 aagccatgcg ccaaaaattt gtcgagttgc tcaaggccaa aggttcaacc caagactttg
    40081 attttattat tgaacaaaac ggtatgttct ctttcagcgg cttgactccc gaacaagttg
    40141 accgtttgaa aaacgagttt gccatttatg ccgtccgttc cggccgcatc aacgtcgccg
    40201 gcattaccga cgacaacatc gattatctgt gtgaaagcat cgtgaaa
//
LOCUS       DAXDSD010000003        39015 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXDSD010000003 DAXDSD010000000
VERSION     DAXDSD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39015
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.003"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(134..1051)
                     /locus_tag="ACFB2Q_000086"
     CDS             complement(134..1051)
                     /locus_tag="ACFB2Q_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC0917211.1"
                     /translation="MNNSNLGLENIGNLSELLGNAEVSSGTPLRLPLASIKEDPDQPR
                     KEFDEGKIQELANSIQERGVKTPISVRQSSEEGVYVINHGARRYRASLLAGLSEIPAY
                     IDQDYSLIDQAVENIQRDNLTPREIADVIGRLLAEGMKKGQIAEKMGKSPAFVTQHAN
                     LLDLPEPLANAFNSGKVSDLTVINDLNALYKKEPEAVEDLLSNEDEITRSSVRNLKEF
                     LNSDPKNSADADHEKPSKAKVKEKKPEDPDKLKKAIVQVMYQGQLARLVLNKRGLSGD
                     EVWIKFEESGETESVPCKEISGIVAVIEG"
     gene            complement(1057..1812)
                     /locus_tag="ACFB2Q_000087"
     CDS             complement(1057..1812)
                     /locus_tag="ACFB2Q_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC0917212.1"
                     /translation="MKILVVANQKGGVGKTAMSLHLAWHMETVGLRVLVIDLDTQGNA
                     SYSLRDKTCLFGSGRLFGNMEDADLHMPGNVSLALSPATNDLANVQNMTLQNAVQSFT
                     QNIDKLKAGGQFDVCIIDTPPSLGNTLAAALAAGDYVLCPIELETYSLQGIKQMAATI
                     GNIRKVNSKLAFLGILPSKVDMRNPRHKRHLEEIKMQYNEIVIPHIIGLRSSIADALS
                     SSLPVWKIKKTAARKAAQEMKAVSNYVLNKMQG"
     gene            complement(1822..2067)
                     /locus_tag="ACFB2Q_000088"
     CDS             complement(1822..2067)
                     /locus_tag="ACFB2Q_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917213.1"
                     /translation="MDRIKSKAGKKMFEVVYMVLAIAYLLFPVRWFAWMYSFYVLCQM
                     LYHWNMADSWIYIGKFILTFGIVMGWLTFVIAFRNKI"
     gene            complement(2148..2495)
                     /locus_tag="ACFB2Q_000089"
     CDS             complement(2148..2495)
                     /locus_tag="ACFB2Q_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917214.1"
                     /translation="MTDLISTTVLLTPYQAFQAKKWHLQNIEEHNLIQKKDLNIRPEC
                     FHRFPQWAVPTADEVKELWLATGMTQKQVSRYLGLKDTNGRAFRRFISGESSITYAYW
                     VLLSLYVGIEKFW"
     gene            complement(3074..3952)
                     /gene="trfA"
                     /locus_tag="ACFB2Q_000090"
     CDS             complement(3074..3952)
                     /gene="trfA"
                     /locus_tag="ACFB2Q_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HFC0917215.1"
                     /translation="MKNVQEVLPYLNLVAERRDEAQREEYLKSKGIPTDDNLIIEDLF
                     GNTIFGSKHRAMPNDFARSCLFTARNHRVPRKTFSREKLFHISEKVEILYTGTELRSV
                     DDELIWMQLVDYCRRFPLGNYVEFDIRQLIKDIGWDTSGAYYKRVRESLSRMKATEIY
                     IKNGNTYGVSGGISLIDNYVGIENIKGEPTRYKVSIDKKLIVLFAGNTFSNIPWSQYK
                     KLSPMARRLTDYVFSHKHPNPIPIPAFLAMCGSDQINSPAKTQNQNAKKICAELTEAQ
                     LVKTAFVLSGKIIVER"
     gene            complement(3956..4339)
                     /locus_tag="ACFB2Q_000091"
     CDS             complement(3956..4339)
                     /locus_tag="ACFB2Q_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC0917216.1"
                     /translation="MNKFIISGRTTKDLTLEQTATGVFTTSIPLAENYFYTKDGKKVE
                     ATNYFWIDVYGEQAKNAVRYCGKGSLILIEGRISNNNYEKDGQKIYKDQMIASRIEYS
                     NLKPPAGREESSDPSFIYDPEQDLG"
     gene            4399..4764
                     /locus_tag="ACFB2Q_000092"
     CDS             4399..4764
                     /locus_tag="ACFB2Q_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC0917217.1"
                     /translation="MSYSKIFFTNVLRLASEKNLSHQDLAERANLSPSSMSGITRGQG
                     NPTLETMSAIAYALDVPLSYLLEHHDLDPESLSLLDGSQSTLPPNYEKITVILPRYKA
                     FQVKKWNDDALKILAKETK"
     gene            4893..5891
                     /gene="trbB"
                     /locus_tag="ACFB2Q_000093"
     CDS             4893..5891
                     /gene="trbB"
                     /locus_tag="ACFB2Q_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HFC0917218.1"
                     /translation="MMETTETADSRKERALNKLKRDLGNEFLEKFMNPNLVEVMFNPK
                     QTGNGVSVYWEEQGKEMVYDERLSDWFKTSRVEAVIKTVAGYHGKVITEDLPILESQF
                     PIDGSRFTAILPPVTASPSFTIRKKPARVFTLDEYVSAGIMTESQRQTIRNAVSEHGN
                     ILVIGGTGSGKTTLINAILYDMSEQNPNERLYISEDTGEIQCHSQNKVQVYTTGSIDL
                     TKLVKTALRYRPDRIIVGEVRGAEALDLLDIWNTGHPGGIATLHANNAALGLERLESL
                     ITRNPAHPAAIRPLVAQVIHYIVSIGKDEKTGRKIREIIKIKGFDEKSGNYVFDYL"
     gene            5993..6376
                     /gene="trbC"
                     /locus_tag="ACFB2Q_000094"
     CDS             5993..6376
                     /gene="trbC"
                     /locus_tag="ACFB2Q_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer system pilin TrbC"
                     /protein_id="HFC0917219.1"
                     /translation="MTLSQSQQKWLFFIGLAVFLLLMSSPALASQGQGGGLPYESWLN
                     NLRQSVTGPVAFALSIIGIVVAGGVLIFGGELNAFFRTLIFLVLVMALLVGAQNMMSS
                     FFGGAEVTAAVQAAQHYLLPLASKG"
     gene            6381..6698
                     /gene="trbD"
                     /locus_tag="ACFB2Q_000095"
     CDS             6381..6698
                     /gene="trbD"
                     /locus_tag="ACFB2Q_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFC0917220.1"
                     /translation="MRAIRTIPIRRVANRPNLFWGGDREMVQVSLLITFVLVVAAQNT
                     YAFVIGLVFWFFALYALRKLAKYDPLLRFVGLRSITKYKRFYPPHSTPFRNNTSTQGN
                     RYK"
     gene            6695..9259
                     /locus_tag="ACFB2Q_000096"
     CDS             6695..9259
                     /locus_tag="ACFB2Q_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HFC0917221.1"
                     /translation="MIIGIISAISVLGLVLCTGLFLKIRAVTNATKLKQHRSKEEGLA
                     DLLNYAAVIDDGVILGKNGSLMAAWIYRGKDMGSSTVAEREDVSLRINQAFVGLGSGW
                     MVHVDAARRPAPNYTEAGRNYFDSYVTEAIDNERRQAFEAIGTLYEGFFVIVATYFPP
                     LLAEKKVAELMFDDDNAKEGKRAQQDRTLSHFKQACANLESNLSISLELHRLCAHKVV
                     LEDGMEITQDDFLRWLHFCITGKNHPINLPAAPMYIDSLIGGQELYTGVVPMIGDKFI
                     QVVALDGFPMESYPGILTRLGEMPIEYRWSNRFIFMDQHQAVADLEKYRKKWKQKVRG
                     FFDQVFNTHSGSIDQDAQAMVDDAEAALAETKSGLVAQGYYTSVVILMHEDRAFLESM
                     ARQVEKEINTLGFNARVETVNTMEAWLGSLPGHGIENVRRPLLNTLNFADMIPTSSIW
                     TGLEENPCQFYPPNSPPLMQVVTSGSSPFRLGLHVGDVGHTLVFGPTGAGKSTLLATL
                     AAQADRYRNMRVFSFDKGRSLETLTRAVGGSHYNIGDEDNTASGLSFAPMQLIQNTSD
                     RAWALSWVEAVMELNGLKVTPQQRNEIETAIKNNAAEQSYSLSDLSNTLQDVEMREVL
                     KAYTIDGSMGYLLDAEQDSLSFSRFTCFEIEELMNLPEKYALPIMLYLFRRIEKALTG
                     DPAVLFLDEAWIMLGHPVFREKIREWLKVLRKKNCLVLLATQSLSDAANSGILDVLNE
                     SCPTKIYLPNAHAREEDASELYRRMGLNARQIEILATAIPKRQYYYTSPYGRRLFELA
                     LDPLTLAFVAVSDPDTLNAMRQTADQYGEDWPIRWLQNRGINIHNYVDVDKYRRKH"
     gene            9259..9966
                     /locus_tag="ACFB2Q_000097"
     CDS             9259..9966
                     /locus_tag="ACFB2Q_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HFC0917222.1"
                     /translation="MGLKDIFRVREKADREMTENPYLNARKQWNFIMGDMVSSRLLWQ
                     WAGMTGLLIGLGGVGGMIYLGSQSKFIPYIVEVNKLGETVAVARADQPQATDERIVRA
                     MLGSFIGNARTVTTDIALQRKYVLNTYAMLNPKDAAAAKMNEWLNGTPESSPFKRAEK
                     ELVSVEIKSVLRQSDNSWQVDWVETTRAHDGKLLKPPQNMRALLNIYYVPPTTEQQII
                     VNPIGMFIRDFSWSNEL"
     gene            9979..10845
                     /gene="trbG"
                     /locus_tag="ACFB2Q_000098"
     CDS             9979..10845
                     /gene="trbG"
                     /locus_tag="ACFB2Q_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HFC0917223.1"
                     /translation="MKRQILSLALITALSTSAFADSYADKYFEGSHNPKLNRHEQKGL
                     GIAKNYSSDGRMAVPPVPGDNGRITFAFGSDPSVVCAVMQVCDIALQPGEMINDLHAG
                     DTARWNITPAISGSNGNERMHILVKPLDVGLQTTLFIATDRRTYHIRLKSHRTRYMPA
                     VGFSYPEDTAARWRAIRANQARQAEAQVLPTGENIQNLDFNYTISGHNPSWKPLRVYN
                     DGTRTIIQMPPTLSQGEAPALLVVRNGENTLVNYRLHEDRYIVDSLFDEAIMITGVGR
                     HQTKVVIRRSRS"
     gene            10842..11252
                     /locus_tag="ACFB2Q_000099"
     CDS             10842..11252
                     /locus_tag="ACFB2Q_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbH"
                     /protein_id="HFC0917224.1"
                     /translation="MKSNVLFVALALAVGGCATGQYGSFVRSDAVSASVTQDVVEKLQ
                     EEFPAANTVFFLNRSKSDTFGTQLDQALREKGFATTAQQGENEKSLNYVLDQVDSDIY
                     RVMIYIDGTPYSRAYRQTENGAVVPTGSWTKRGD"
     gene            11256..12695
                     /locus_tag="ACFB2Q_000100"
     CDS             11256..12695
                     /locus_tag="ACFB2Q_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC0917225.1"
                     /translation="MSQSTEDLLNASRSPSIAPDTSGRRPGVRRLNNIPIYIVGGVVL
                     GFALLIALVAATKGQQSAGQATQGDGKDATRMADSLLNELPQEGGLITAKASEPAMPA
                     LPDGMASAPDLPASAPTSLPMVPDMANLPATPPEVDQEAEQIRQAKAQMFQAALAAPT
                     KVDVVASTSRGSSGLYTAALSSSGSGTPNRSEMLAQIAEVQRMSENAGSNPAEVERVY
                     NQQLANAQQMAAAGGQDGVQGAGGMPGLNTPPTPGNIPRQTAQNGGNNWRLESQVERP
                     NAYMIRSGAVIPATLISGINSDLPGMVQAQISQNVYDTATGRHLLLPQGSRLVGTYSS
                     GINLGQRRLFIAWNRIIFPDGKALDIGDMPGASGAGYAGFSDRINNHYGRLWGNALML
                     SLVGASITYGVDKNSNNNNNGNNNGNNSTVRGALSESLGQTFGQAVSQSIQKNMNVSP
                     TLEIRPGYRFNVILTKDIDFSKPYRSFDY"
     gene            12708..13484
                     /gene="trbJ"
                     /locus_tag="ACFB2Q_000101"
     CDS             12708..13484
                     /gene="trbJ"
                     /locus_tag="ACFB2Q_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFC0917226.1"
                     /translation="MQKRLLSKTKTVALSLAVSVSLGLSAPAYAGIPVTDGGNIAQSS
                     VSAIQNVAQTLKQIEEYRIQLKQYETQLRNSLAPAAYVWDQANKTMANMKAAVARLRA
                     YKQQLGNMEEYLSKFQAVDFYKSSPCFTAKGCSAADRAKLEQNYAQLSRMQKLSNDGL
                     LRSINNEIDSISRDANTLTQLQRNAQSATGQLEALQYANQFHSAQAHQLLQLRNMINT
                     EMQANAAYRQSQLDREARYEAARKQVRTSDILKHPYRRGQ"
     gene            13719..15326
                     /gene="trbL"
                     /locus_tag="ACFB2Q_000102"
     CDS             13719..15326
                     /gene="trbL"
                     /locus_tag="ACFB2Q_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HFC0917227.1"
                     /translation="MKKKYIYYFIFFITLSMPIISHAAGQGGVLDEALSRYEGKIGAW
                     EAAFYKAGLFIFWSLSSISIVMTGAQLIFQRDNISSFFAEFTRLILFLGFFLWIITNG
                     IKITTSIKDGLMKLAGQASRNGEQITPSSIVDIGFNLFDKIVENSSIWDPIDSATLIV
                     IGLVILLILAAVGINMLLMLITTYIVIYSGIIILGFGGGRWTSDMAVGYLKQVLNLSL
                     QLAAMILIIGIGQSIVQDTINTIGTPGFRELAVVLVQTALLVGLVVKIPPMIGSLAGG
                     AGSGGIGSFGVGNAIAAAAMLAGAAAGAGAALKSLAVEAGGLKKAFEAAKAGLGGSNG
                     SSDSGSGKMDSAVDAVAGANAGDKSNQDGKPLTAESSGNSENGSEGSTTGNSGGNPSG
                     SSEENTISSGSSSSSGEANSTNTSASSGAEGSAEGEATSSETSQSASDSPSNNSGEAT
                     QTADTTPKTTNTGGGSPLATAMGGNKVSNWQAAKNVAVGLVSSKWNNAVNNSAGGRLV
                     KHWEDEKKKTEDPTKNPQKNPEGDDNA"
     gene            15319..15891
                     /locus_tag="ACFB2Q_000103"
     CDS             15319..15891
                     /locus_tag="ACFB2Q_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFC0917228.1"
                     /translation="MLKKTLICILLVGSVHFAMAESLNDALNRSRSGNSNNNRPQVED
                     LFTGDVKLACEATLCLSTGNRPSECQPAIHRYFSIRHKRMGDTIRARHNFLRLCPASK
                     EQGMLELVNAIANGAGRCDARELNRINRSTYIKRVWVGSRRHGDWQEVKVPYIRNAKP
                     SYCSAYFENGWTQLGTRYVGEEKNGGKWVD"
     gene            15891..16490
                     /locus_tag="ACFB2Q_000104"
     CDS             15891..16490
                     /locus_tag="ACFB2Q_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbN"
                     /protein_id="HFC0917229.1"
                     /translation="MVYVADLPPFMQERVTCSIAAAEKYHLPPHYLLAVAERENGRPG
                     QQVRNTNGTYDVGAMQINTAYIRTLSRYGVTQSAVAQPGCYAYYLAAWRIKRHIVYDR
                     KGDIWTKIANYHSYTPKYNAIYRAAIIRLAEKWERWLAGRQGNNAKGNVPTQAVPAVR
                     TIRSQPLPKQYVSPTTVYVPRTIRVVERSPEPEPETVQN"
     gene            16599..16883
                     /locus_tag="ACFB2Q_000105"
     CDS             16599..16883
                     /locus_tag="ACFB2Q_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFC0917230.1"
                     /translation="MAYAIAFDLDTTVLQELYPNSSWQNAYGDVKRTLVGLGFNHQQG
                     SVYFGNSEITAVSCVLAAQKLSQTYAWFKPAVSDIRMLRIEEMNDLQPAL"
     gene            complement(16993..17547)
                     /locus_tag="ACFB2Q_000106"
     CDS             complement(16993..17547)
                     /locus_tag="ACFB2Q_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFC0917231.1"
                     /translation="MIIGYLRVSTKEQNVAAQRQVIQDKYQIERWYEDQAASGAVKAL
                     NRPEFRKMAEFVRQGDTVVVVAIDRLGRNTIDVLETVQFLQKKGVAVTSLRENFDLAS
                     PLGTAMLAIVSAFAELERSYIKERQMAGIRNAREEGKKLGRKVKVDPEEIRRWRQSEQ
                     TSISRTAAHFNVSESTVKRACKAK"
     gene            complement(17599..18030)
                     /locus_tag="ACFB2Q_000107"
     CDS             complement(17599..18030)
                     /locus_tag="ACFB2Q_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC0917232.1"
                     /translation="MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLA
                     DILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVRLSPFLNGCPHDLSLGAESLGKG
                     ESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ"
     gene            complement(18045..19250)
                     /locus_tag="ACFB2Q_000108"
     CDS             complement(18045..19250)
                     /locus_tag="ACFB2Q_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC0917233.1"
                     /translation="MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQH
                     ADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHNSAAVLSSTQFDEVLKRDEPQKK
                     EVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
                     VSMRAERASDNTYKRFNEYGRGASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQD
                     RNSEFIDKFDDVIKMLSLGSQEQILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESV
                     FAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGIYLLGQAKKAETGQT
                     YSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLE
                     YSAKKSISR"
     gene            complement(19257..19442)
                     /locus_tag="ACFB2Q_000109"
     CDS             complement(19257..19442)
                     /locus_tag="ACFB2Q_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917234.1"
                     /translation="MNKVEPQESNAIKMIKEACEKNRRMMTDEAFRKEVEKRLYAGPS
                     PELLAKLRVLWAANKEQ"
     gene            complement(19476..20684)
                     /locus_tag="ACFB2Q_000110"
     CDS             complement(19476..20684)
                     /locus_tag="ACFB2Q_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFC0917235.1"
                     /translation="MSDYQKLSDAGRAEIVAEYMSALLEITQAVDVPQIALVAAQPGA
                     GKSKAADIVKEEFASKGGHIHVDADIMRQKIPVPPGVVYSSQQTQEDAGKLAVGVRKS
                     ALENSRNVLEEGTFRNAEAVGMSIKAAREAGLKIEMLAVATAPEESLAGIFKRYEDQY
                     LTKNIQPRFVDEDFHNKAFEGFKNTVATHEAEFDRIRVTNRPGEILYDSLNKQQNKQA
                     SAKDAMEFYQQITPERLKQVAQVWDVIQLQADRRSQDPVPNYFDKVKQHREEIYQRVE
                     EIYRQERVVANSEGATLQRKSGDTWQDIEKAEAKGMKAGIHMLGTAKPAESGKEYSGE
                     IVHKDEASVFQKTDQGLIRHKAVQGMSEGKFSSLSEQVEIGQKVSIKREGNGLSVKAS
                     DASVKKTMKR"
     gene            complement(20681..20929)
                     /locus_tag="ACFB2Q_000111"
     CDS             complement(20681..20929)
                     /locus_tag="ACFB2Q_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917236.1"
                     /translation="MKEVIPGINYEVALEVLGNAAQPFIQAREEERKKSHPNEAFIQY
                     CSDSIKAITVFRHNLRATDEDVIARILDPNDTIVGGSV"
     gene            21414..21887
                     /locus_tag="ACFB2Q_000112"
     CDS             21414..21887
                     /locus_tag="ACFB2Q_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917237.1"
                     /translation="MPHEQMIDAFSAIIQNSLSLYEEQTPLYYYLTICGSWVLTALGI
                     LSLASVFFTIKQKKPYRFIVPILFLWLGYESIHMPRPNLAVFYGYNLAQLSYPANENP
                     NSFLHLADGSNPICCFIGEDEYLQAKRLGFKEYADAKAKALVDLVEMEKKSKQSD"
     gene            complement(22039..22935)
                     /locus_tag="ACFB2Q_000113"
     CDS             complement(22039..22935)
                     /locus_tag="ACFB2Q_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine modification methylase"
                     /protein_id="HFC0917238.1"
                     /translation="MFTHSVCSYPQRGNYGKSGYRGNCTGHIIRDFVESYLAPEGLFA
                     DPSIGSGTSSDVAEEMGIRFKGTDLHQGYNLLTQDFAAFLGEQANLIWWHPPYWNMIR
                     YSGNQWGDAANPWDLSQMDLEQFQEAMLLAMMNIHDATEQGGHYGILMGNMRKDGRYY
                     NLSSMVERMAAGRLVDEIIKIQHNCVSDSRQYGGKLVRIAHEKLLVFKKEIQTALYFL
                     AKAMNRVEAVTAVTWKAAVRRVLQAADGRNMGLTEIYGEMEPYAAARAENRNWQAKVR
                     QVLQDERFFLRVGKGIYALKTE"
     gene            complement(22939..23280)
                     /locus_tag="ACFB2Q_000114"
     CDS             complement(22939..23280)
                     /locus_tag="ACFB2Q_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917239.1"
                     /translation="MLYWDDVSDSFDGWMNLLRQLGGDSFINFEQDIWETARTYETVP
                     HFGNLRQHHLLERLQDTIRNRWPFLRTGFLINALDTHFYVNDEPVETMRDLDAVLGCH
                     YRENEDDLKEE"
     gene            complement(23294..23608)
                     /locus_tag="ACFB2Q_000115"
     CDS             complement(23294..23608)
                     /locus_tag="ACFB2Q_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917240.1"
                     /translation="MASYTNRLTGHPNVFVEQNIWSNGELMGFSPINVMWDGRNAPTL
                     LCRYTFDGGQYYSLQVSEAAELETRGYQIVCDDLQCLKLKTAKARRSGILALILADAG
                     IE"
     gene            complement(23975..24334)
                     /locus_tag="ACFB2Q_000116"
     CDS             complement(23975..24334)
                     /locus_tag="ACFB2Q_000116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917241.1"
                     /translation="MSLSAENRTFYLVETGEIGFGSSALFISQSLEEAEAVVADFFKG
                     DEKLHYETDGNQPEISLGMGEPQEEEYIPFLPREPFFWTKGGFGMMGTEYVTIRKTIN
                     GYLEGAGSFRLIKSQQD"
     gene            complement(24331..24765)
                     /locus_tag="ACFB2Q_000117"
     CDS             complement(24331..24765)
                     /locus_tag="ACFB2Q_000117"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC0917242.1"
                     /translation="MSIYRPASRERKNIKFFADGQYIQEGYKALCWMMRDVVDNHQMH
                     AININLINLLFAQQQYLRDLGRPNPELVLHSGFRTRRHNDSLEGAAKNSQHLSGNAGD
                     FHIERASLSELAALARRFRVGGIGIYPTFIHNDIGVYREWRK"
     gene            complement(24838..24891)
                     /locus_tag="ACFB2Q_000118"
                     /pseudo
     CDS             complement(24838..24891)
                     /locus_tag="ACFB2Q_000118"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
     gene            complement(24888..25382)
                     /locus_tag="ACFB2Q_000119"
     CDS             complement(24888..25382)
                     /locus_tag="ACFB2Q_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
                     /protein_id="HFC0917243.1"
                     /translation="MDKDKLYRVAFFLTYHKQAREGWFWRVFGMAFDGRLRQTLQNIA
                     VDYRKTSQNQPLAFDDYLEMLQRHEEEKMGVSAERNQSEQAAVKAFQKQYGLNHRVDT
                     HADTKTLSITITATDEDFIPAVEMGLEQVLGSLAAGETANEGGNEYGRYAFMVSGNVK
                     GRER"
     gene            complement(25391..28390)
                     /locus_tag="ACFB2Q_000120"
     CDS             complement(25391..28390)
                     /locus_tag="ACFB2Q_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014579490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HFC0917244.1"
                     /translation="MQNKKPFYEQVAEKLIEQLRQGTAPWQKPWDGGGLPDFMPHNAI
                     TGRRYRGINNLWLQAQGRKDPRWITYKQASSIGAQVRRGEKGTVVEYWQFTEEVPKTD
                     EKGQEIIGQDGKPEKETVRLERPKVFHAVVFNGEQIDGLPPLKLRPLSWNPDERAENI
                     LKNSGAVIDHRHGDRAFYSPSRDSITLPLREQFESPGAYYETALHELGHWTGHADRLN
                     RDLSHPFGSEGYAREELRAEIASLMLSQELGVSFNPGQHASYVASWIMVLQDDPMEIM
                     RAASDAEKIQGYVMAFDQVREIEQQNEAAVSISDRQNIDEASLAEEKNLINAVDKNDV
                     AAIINFMAEEKMHEGLKRFSLVISEMDDATFQNAFWQRHQKPENGESFMENLNAAKEA
                     LDEAIEQSSYRTVEEQIRQFLPEQDKESVRVVSDERVYVQIPYADREQAKMLGAKWDK
                     EEKSWYIPPGVNQEPFRKWLDSATNQAKTVTDNTPAIAKTLIDVPFREKNEARALGAK
                     WDKDQKSWYIPEGVNTALFARWMQPKQEALAEEAVPDDTPQEEAVECQVAAIQQEYQA
                     AKTEEAPAEENASRVYLAVPYEEKEEAKALGAKWDKANKSWFMESSNANLPAASKWLP
                     EKQQTQSMPAMSPVEEFADKLREMGCVVANGHPFMDGQKHRIETVGDKKGERSGFYVG
                     HLDGHPAGYIKNNRTGAEVKWKAKGYSLSPEEKAKLAAEAAQKLQMRSEQKKQAQEAA
                     VGRIKYYMESYLPVQTGTPYLNAKGIKPQPGIFTDREGQKTFIPVYNAAGELRSMQYI
                     QADGTKRFAKDSEQEGCMHVVGQQDLAKAKTIILSEGYATAASIKEATDDTVASVAAF
                     NSGNLPLVAKVLSAKYPQAQFLVAGDDDLAVEAKQGNNPGKEKALEAAKILNCRAVFP
                     VFAPGEQSSEHKAFTDFNDLAQKSKFGREGLAKQINEAIHARPANELQTLKTRGLQEE
                     ASQDRPVEQTKRQQRATTRKTASRGSR"
     gene            complement(28393..28557)
                     /locus_tag="ACFB2Q_000121"
     CDS             complement(28393..28557)
                     /locus_tag="ACFB2Q_000121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917245.1"
                     /translation="MIAEKVADLNNPGMEVDFDPAEAEALGAFEETAMDAETARDSVA
                     DLSREVAEGA"
     gene            complement(28579..30774)
                     /locus_tag="ACFB2Q_000122"
     CDS             complement(28579..30774)
                     /locus_tag="ACFB2Q_000122"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC0917246.1"
                     /translation="MSRLYIAEKPEVARAIAAALGVGIERRNGYFRCSNGDNITWCYG
                     HMLALCDPEDYDSKYGNWKFEDLPIAHIPWRKKPLSDKKEQIGVIRDLLAECTEIVHA
                     GDPDDEGQLLVDELLEFLNCAKPVKRVLINDNNERSVKRALSAIEDNAKFRGLSQSAE
                     ARSVADQLYGYNMTRAYTLKAAEQGYQGVLSVGRVQTPILGLVVRRDRENQSHVKTHY
                     YHVYADLDVDGVRFTARYVPNKDQDPIDDKGRLSDPLFARQIAEAASGQPVKILAAKT
                     SAKRQSPPLPYNLLKLQADCSRKFGLKPDQVKDIGQSLKDKHRLITYHRSDCQYLNDE
                     HFDDAADVLDAVGKTASVFKNAIASADTKIKSRAFNPDKVSAHHAIIPTVKTANMDDL
                     TEDEKRVYLLIARAYIAQFWPDHEYNQTDIEAEIAGKKFVCRSNVTVKPGWMALYKND
                     QGNAETATDPEDLSVDLQMLKPGNSGRCLTAEETQFETKPRPLYTMDTLLNDLTRVAK
                     YVQNPKLRELLIEKDKGKEGEHGGIGTPATRDEILRNLLDKGFLLEKGKNIVSSEIGQ
                     QLYDALPDQAKFPDMTALWHEQQQHIVQGGLDAQRFVNSLMEYISGEVERVGREGISL
                     NVKKYPCPECGKPLRRMRGSKGFFWGCTGYKDGCKVSFSDKAGRPVLAPKKAEPSSKF
                     KCMACTHPLVRRPGKQKGTHFWGCSNYPECKQYYPDAGGKPDYSKHTKG"
     gene            complement(30777..31313)
                     /gene="traF"
                     /locus_tag="ACFB2Q_000123"
     CDS             complement(30777..31313)
                     /gene="traF"
                     /locus_tag="ACFB2Q_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer signal peptidase TraF"
                     /protein_id="HFC0917247.1"
                     /translation="MRRLFDRAMLAMCLTAALLVTGYGLLYAAGFRINDTRSVAKGVY
                     RISGEAVRRGSYITFCPPDTEVFRMARRRGYIRAGFCPENYSGLMKFVAGIPGDNYRF
                     TDEGLLINGRLLGGTRPLWQDGLGQPLPVFEGEGRLKDDEYILIGNAVANSFDARYYG
                     IVRREHIRDVVKPIWIEE"
     gene            complement(31310..33199)
                     /locus_tag="ACFB2Q_000124"
     CDS             complement(31310..33199)
                     /locus_tag="ACFB2Q_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFC0917248.1"
                     /translation="MFRSKNKRNNAVGHQNQAQATGSGRLVNFICLVLVLLAGMGAAT
                     QYFAHQFQYHSQLGYGIGKFYMPWSILIWYGKWHGRYEQQFMTAFSIMLMVVAVGMIG
                     LLVWNIVRKNTNKAHESLHGSARWADRKDIEAAGLLAKKGEDSDGVYVGGWVDKKGTL
                     HYLRHSGPEHVLTYAPTRSGKGLGLVLPTLLSWPHSTFITDLKGELWALTAGWRKKYA
                     HNKVIRFEPASAQGSAAWNPLDEIRVGTEYEIGDVQNLATMIVDPDGKGLNSHWDKTA
                     FALLTGVILHALYKARDDGGVATLPSVDRMLSDPEKPVGDLWVEMATYPHADGRPLPV
                     ISSAGQDMLDRPEEEAGSVLSTAKSFLALYRDNVVAANVSRSDFKIKDLMNDEDPVSL
                     YIITQPNDKARLRPLVRILINMIIRLSADKMDFENGRPKVHYKHKMLAMLDEFPSLGK
                     LEIMQESLAFIAGYGIKCYLICQDLNQLKSRETGYGPDETITSSCHVQNAYPPNRIET
                     AEHLSKLTGITTVVKEQITTSGKRVSAMLGQVSRTTQEVQRPLLTADECMRMPGPVKD
                     ARGLITQAGDMVIYVAGYPAIYGRQTLYFKDPVFEARSKVPPPRHTDRTQKKTANTPS
                     AFDTL"
     gene            complement(33216..35609)
                     /gene="traI"
                     /locus_tag="ACFB2Q_000125"
     CDS             complement(33216..35609)
                     /gene="traI"
                     /locus_tag="ACFB2Q_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI/MobA(P) family conjugative relaxase"
                     /protein_id="HFC0917249.1"
                     /translation="MIAKKIPMRKANLSSFEGLVTYLAGSQGVANRVGKVEIHNYESS
                     DLRWAVQETLAVQDQNKRSAADKTYHLMVSLAGGEFLTDEQLSRIEQSMIEALGYAEH
                     QRISVVHRDTDNLHFHVAINKVHPEKKTIHEPYNDFKILGRECRKFEEEYGLQRVQRG
                     GRGNKKIDMEEVVGIESLIGWIRRECGEQIKEAKTWEQLYHVLSDHGLEIKLRGNGFV
                     ISDGEHHVKASSVDREFSKSALEKRLGAFDQKTGVKAKPSKTYREKPLSKANVDDLFR
                     QYKEEKELQKSRRQDFFLHLAQNRDMEIGRIKEKYAFRRSLIRHSSSGIARRILYRML
                     FRQMKDEIRSVRTDYARRRAVFFEQNKGFAWADWLKHRAAEGDVRALDALRRRGKDAG
                     YKGNRFVGKESGGFAGIKVDTVTKKGTVIYAEDGMTIRDTGSVLKLAAIPDDKAVSRA
                     LEIAIGRYGKVLTIGGTRHFQDKVARIAAAKGLDVSFTDGRLNHLYQQYVKEYEYVRE
                     RTDRYRQADGTGRADGRAGQQRGGAGYAGGKPRRYRWHGYDASRNRGFHAGISGYPIP
                     KLSDSGGIGAGRGQSASAGSDSLRGMPERSLVRIQGRTQVLLPRDVSSHLEQQGSANY
                     QAVRRTIHRTGGVAEIRQMQAVVGLVAKPGKKPPGFRRGSLRGMEGLAEIKISSAAVV
                     EIPKNPEPAQENERLTHSLAPIDKYIAERQEKIDKKFDILNHRKYNPSDSGVMLFRGI
                     RNIDGQALALFQPKEVEEILVMSVTPYVSRRLSSVKLGSEVKVTPTGQIRPVKGRHR"
     gene            complement(35612..35989)
                     /locus_tag="ACFB2Q_000126"
     CDS             complement(35612..35989)
                     /locus_tag="ACFB2Q_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFC0917250.1"
                     /translation="MAGARKRPKYISVYVSEEEWKTIESLAKSVGLSQSAYLKLLGLG
                     YKPASVYDGEAVKALAKINADQGRLGGLIKMWLANDERLMMFDHDQLAKAIFGVLEQI
                     GEMQESMLEIVRSRRILAAKKIG"
     gene            36039..36758
                     /locus_tag="ACFB2Q_000127"
     CDS             36039..36758
                     /locus_tag="ACFB2Q_000127"
                     /inference="COORDINATES: protein motif:HMM:NF036299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraK family protein"
                     /protein_id="HFC0917251.1"
                     /translation="MASLAEQDNPLSLPANKDCDVHVHHISCPNSGVHPNSGVQYAKD
                     YTPNNSIYRTYFDDKPPKNALSQTKNDLLYRIIVFKTILLGSFGKALSKNWQSPIEMI
                     QMGKIEKLVLAELAAMQKKGSSAGNKARFLAMQEDIKILLVDRNIPVISVWRILSEKK
                     YVPYSYETFNRYCKMYIFKSDTTAADQTDTAQSTQEKPDSIRTDKEPPSPASEPIIAG
                     GNTKSSSGFTFNNNPDGKDLF"
     gene            36759..37484
                     /locus_tag="ACFB2Q_000128"
     CDS             36759..37484
                     /locus_tag="ACFB2Q_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraL"
                     /protein_id="HFC0917252.1"
                     /translation="MAIIHISLQAKGGVGKSFLALMLSMFYRHIERPVKIFDTDPANA
                     TLFGFPALEAEKFEIMKKDDIDKRLFDRLFSQILSATDDVIIDVGANAFIPLCSYIIS
                     NNLITMLTQEGHQVIIHTSIVGGQNLKHTVNGFDALCSQFPESTGFVIWLNPFWGEIE
                     FEGKSFEKLKVYEKHKSRIRSIIKIPEWDASLHGRDLAAMLEKQKTFDEALASEDFSI
                     FERQRLNIIKNEMFQTIETAQIV"
     gene            37492..37974
                     /locus_tag="ACFB2Q_000129"
     CDS             37492..37974
                     /locus_tag="ACFB2Q_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917253.1"
                     /translation="MDEKVKAILATIVEKNGIALSPKDPIMVVFTALEYMLSEQHAEQ
                     KALTVLFKAQVEEATGNWIQSVHQQTEELVSQQLNTTKEQAKEMSKLLLHETDRLLEQ
                     RITDAVKDFEGKLYGINDNAMNAVELRIKETRNTAILNLTAALIVLLAVIILAFKFTF
                     "
     gene            complement(38031..38567)
                     /locus_tag="ACFB2Q_000130"
     CDS             complement(38031..38567)
                     /locus_tag="ACFB2Q_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncP plasmid survival protein KfrC family
                     protein"
                     /protein_id="HFC0917254.1"
                     /translation="MNEDKGLVAKAVAAETEQEELFESRQVIEETFAGVLNTYVLEKT
                     EQVERIEEKLEQLISGQMAKLQALQNARPGMLSMPSKKQTWSANIAVAQSRLNRLHDR
                     LEDVREIRDGMALGVPKIQELAARKLRMEEPDLAKKFDDVQTAVRVHNLHEKQRKEKE
                     QQKRQAGLSLSLGQKIVR"
     gene            complement(38604..>39015)
                     /locus_tag="ACFB2Q_000131"
     CDS             complement(38604..>39015)
                     /locus_tag="ACFB2Q_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="KfrB domain-containing protein"
                     /protein_id="HFC0917255.1"
                     /translation="LKTVLLELFTSFLKIFSRMEAEKQRVLVLNKQHLFETEKDGKWI
                     TQAVKPAPANMKPGIYLLHTAKSPQDSVRYEGQIVFQNDSYVYQKTRQSIVKYPCSLF
                     SSVPSIGEVVSISVKDKAATVEKAGIGEKKTLTR"
BASE COUNT         9599 a        10389 c         8600 g        10427 t
ORIGIN      
        1 aaatatttga ttttaaaatg atttaattta atgaattctg atcaacctgc tgattcggta
       61 ttttgcacat accggcagca gtaaaactgg gcagcccgtt aaaacggggg ggtattataa
      121 taaattccca gtattatcct tcaataactg caacaatccc ggaaatttct ttacaaggaa
      181 cagattctgt ttcgccggac tcctcaaact taatccatac ctcatcgcca gacaatccgc
      241 gcttgttcaa aaccagcctt gccagttggc cttgatacat tacctgcaca atagcttttt
      301 tcagtttgtc aggatcttcc ggctttttct ctttcacttt tgccttggat ggcttttcat
      361 gatcggcatc agcagaattt ttaggatccg agtttaaaaa ctctttcaaa ttccttaccg
      421 aactacgggt aatctcgtct tcgttagaca gcaaatcttc caccgcttcc ggttcttttt
      481 tatacaatgc gttcaggtca ttgattaccg tcaaatcgga tactttcccc gagttgaatg
      541 cgttggccag aggttcgggt aagtccagca ggtttgcatg ctgggtaacg aaagcagggg
      601 atttgcccat tttttcggca atctgtccct ttttcatccc ctctgccagc aatcggccaa
      661 ttacgtcggc gatttccctt ggcgttagat tatcgcgctg gatattttca acagcctgat
      721 cgatcaggct gtaatcttga tcgatatatg cgggaatctc gctcagccct gccagcaatg
      781 aagccctgta acgtcgtgcg ccgtggttga tgacatagac cccttcttcg gaactttgac
      841 gcaccgatat gggtgttttc acgcctctct cctgaatgct gtttgctagt tcctgaattt
      901 ttccttcgtc aaattctttt cttggttgat cggggtcttc tttgattgaa gcaaggggca
      961 ggcgcagcgg cgtccctgat gagacctctg cattacccaa aagttcgctg agattgccga
     1021 tgttttccaa acccaaatta ctgttattca taaattttac ccttgcattt tgtttaaaac
     1081 atagttagaa acggctttca tttcctgcgc cgcctttctt gctgccgttt tcttgatttt
     1141 ccaaaccggc aggctggaag acaacgcatc tgcgatactg ctcctcaatc cgataatatg
     1201 cggtattaca atttcattat actgcatttt aatttcttcc aaatgccttt tatggcgcgg
     1261 attcctcata tcgaccttac ttggcaatat acccaaaaaa gccaatttgc tgttcacttt
     1321 tctgatattg cctattgttg cagccatctg tttaattcct tgtaggctgt atgtttccaa
     1381 ctcgatcgga cataacacat aatccccggc cgccagtgca gccgccaacg tgttacctaa
     1441 actgggcgga gtatcaataa tacagacatc gaattgcccg cccgctttta atttgtcgat
     1501 gttttgcgta aaagactgaa cagcattttg caaagtcata ttctgcacgt tagccaaatc
     1561 gtttgtcgcc ggggaaagag ccagtgaaac gttgccgggc atatgtaaat ccgcatcttc
     1621 catatttcca aacaaacggc cggaaccgaa aagacacgtt ttgtctcgaa gagagtaact
     1681 cgcattcccc tgcgtatcca aatcaattac taaaaccctc agtccaactg tttccatatg
     1741 ccaggcaaga tgaagcgaca tagccgtttt tcctaccccg cccttttggt ttgccaccac
     1801 taaaatcttc attcctacac ctcatatttt atttctgaac gcgataacaa aagtgagcca
     1861 tcccattacg ataccgaacg tcaagataaa tttgccgata taaatccatg aatcagccat
     1921 attccagtgg tacagcattt ggcacaaaac atagaaactg tacatccaag caaaccatcg
     1981 gacagggaaa agcaaatagg cgatagctaa caccatataa accacttcaa acattttttt
     2041 tccagctttg gatttaattc tgtccatagg agcctccgtc cgatatagtt tgtattatag
     2101 gataaattat cctatttgtc aagcaatttt agcggctaaa attttattta ccaaaatttc
     2161 tcaataccca catacaagct caataaaacc caataggcat atgtgatgga tgattcccca
     2221 ctgatgaatc tcctgaaagc tctaccattg gtgtctttca accctaaata cctgctaact
     2281 tgtttttgag tcattcccgt tgctaaccac aattctttga cctcatcagc cgtagggact
     2341 gcccattgcg gaaaccggtg gaaacattct ggacggatat tcaggtcttt tttttgtatc
     2401 aagttatgct cttcaatatt ttgaagatgc catttctttg cttgaaatgc ttggtaagga
     2461 gtaagtaata cggtggtaga aattaaatcg gtcatttgaa agttctcggc aatttaccct
     2521 tggagtaaaa tgaaaaagat ctaaatcgcc cgtagggcga cagacgaatt gataaaaccg
     2581 gcttttcggt cgcgaaatat ggttataaat ggttagataa tggttagggc aaaaaaaagc
     2641 ggtttttttc aattaatatt cagtatgttg caaacatggc ggcatgtgta aatccgcgat
     2701 ttagcatgtg taaatccgcg atttagcatg tgtaaatccg caagggaatt aggatatttt
     2761 gtcctaaaag catgtgtaaa tccgtaaatt ttgctcatat tttaggcgat accgaatgtt
     2821 atgcacaggt tatccacagt tttgattttt aaaataggtt ttttatattt attttgtctg
     2881 tttgtttttt aatttttatc gcatatcttg acctgcatat tttggtattt ttgtcaaacc
     2941 ggccatcggc atgtgtaaat ccgcaaatgg catgtgtaag tccgcaaaca gtgaaaattt
     3001 gctgtttttt ggcatgtgta aatccgcaaa tggcctgttt ggggcatgtg taaatccgtg
     3061 cttgtggata attttaacgc tcgacgataa tcttcccgga taatacaaag gcagttttta
     3121 ccaactgcgc ctcggtgagt tcggcacaaa tctttttagc attttgattt tgcgttttcg
     3181 ctggggagtt gatttggtcg ctgccgcaca ttgctaaaaa tgcaggaata ggtatagggt
     3241 tgggatgttt gtggctaaag acataatcgg tgagccgcct agccattgga gaaagttttt
     3301 tgtattgact ccaagggatg ttggaaaatg tgtttccggc aaaaagcacg attagttttt
     3361 tatcgatgct gactttgtaa cgtgtcggtt cgcctttgat gttttcaatt ccgacataat
     3421 tgtcaataag ggaaatacct ccgctgacgc cgtaggtatt tccgtttttg atatagatct
     3481 ctgtcgcttt cattctgctc aaagattccc ggacacgttt ataataggct ccgctggtat
     3541 cccatccgat gtccttaata agctgcctga tgtcaaattc gacgtaattc cccagaggga
     3601 aacgacggca gtaatctaca agctgcatcc atatcagctc gtcatccact gaacggagct
     3661 cggtacctgt gtaaaggatc tctacttttt ctgagatatg gaaaagtttt tcacgggaga
     3721 aagtttttct cggcacccga tgatttcttg ctgtaaacaa acatgatcgg gcgaaatcgt
     3781 tcggcatcgc tctatgcttt gaaccaaaga tagtatttcc aaacaaatcc tctataataa
     3841 ggttgtcgtc tgtcggaatg ccttttgatt tcaagtattc ttccctttgg gcttcatccc
     3901 tgcgttcggc gactaaattc aaataaggaa gcacttcctg aacatttttc ataatttatc
     3961 ctaagtcttg ttcggggtcg tagataaatg aggggtctga tgattcttcc cgtccggcag
     4021 ggggtttgag gttgctgtat tcaatacggc tggcaatcat ctggtcttta tagatttttt
     4081 gcccatcttt ttcatagttg ttattgctta tccttccttc tatcaggata agggaacctt
     4141 tgccgcaata cctgacggcg ttcttggcct gttctccata tacatctatc caaaaatagt
     4201 ttgtggcctc aactttcttc ccatccttgg tgtaaaaata gttttctgcc aaaggaatac
     4261 tggttgtaaa aactcctgtt gcagtttgct ccaaagtcaa atctttggtt gttctgcctg
     4321 aaatgatgaa tttattcatt ctgcctccta ggttactttg ctatacttac ggttaaaaat
     4381 tttagcggct aaaattccat gagttattcg aaaatattct tcactaatgt tctgcgtctt
     4441 gcatccgaaa agaatctgtc ccaccaagat ttggcagaaa gggcgaattt gtccccgtct
     4501 tcaatgtcgg gaattacaag agggcagggc aatccgacat tggaaaccat gtcggccatt
     4561 gcttacgccc ttgatgtccc gttgagctat ttgctcgaac accacgatct tgatccggag
     4621 agcctttccc ttctagatgg aagtcaatca acactcccgc ctaattacga gaagattacg
     4681 gtcattttgc cgcgttataa ggcattccaa gttaaaaagt ggaacgatga tgccttaaag
     4741 attttggcaa aagaaaccaa atagcaacat tcattaggtt attttatccc atatctgcca
     4801 gatatgggct ttttttttgg ctcttgaaca attagacttt acattttatt gaattcaata
     4861 tattgtatca actctattgt atggttctct taatgatgga aacgacggaa acagcagact
     4921 cccgcaaaga acgggcattg aacaaactaa agcgtgattt gggaaatgag tttttggaaa
     4981 agttcatgaa tccaaacttg gttgaagtga tgttcaatcc aaaacagacc ggaaacggcg
     5041 tgtctgttta ctgggaagag caaggcaagg agatggtcta tgacgagagg ttgagcgatt
     5101 ggttcaaaac ctcccgcgtg gaagcggtta ttaaaaccgt tgccggttat cacggcaaag
     5161 taatcaccga agaccttcct atcttggagt cccaatttcc catcgatggg agtcgtttta
     5221 cggccatctt gccgccggta accgcatcac cctcttttac catcaggaaa aaacctgccc
     5281 gtgtattcac tttggatgag tatgtctcag caggaatcat gacggaaagc cagcggcaga
     5341 ccatccgcaa tgcagtcagt gagcacggaa acatcttggt tatcggcgga acaggttcag
     5401 gtaaaaccac gttgattaac gccatcctct atgacatgtc ggagcagaat cccaatgagc
     5461 gtctctacat ctctgaggat accggggaaa tccaatgcca ttcgcaaaac aaagtgcagg
     5521 tttacaccac gggaagcatc gatctaacca aacttgtgaa aaccgcgctc cgctaccgtc
     5581 ccgacagaat catcgtaggc gaagtaaggg gggcggaagc cttggatctt ttggatattt
     5641 ggaacacggg acacccgggc ggcatcgcca cactgcatgc aaacaatgcc gccttgggac
     5701 tggaacgctt ggaaagcctg attacccgca atcctgccca tccggcagcg atacggccgc
     5761 tggtggcgca agtgatccac tacatcgtat ccatcggcaa agacgagaaa accggccgca
     5821 aaatacgtga gatcatcaaa attaaggggt ttgacgaaaa gtcaggcaat tatgtgtttg
     5881 attaccttta agaggaacag ggaggcaatt taaacagcgg gattgaatac tgatgttttc
     5941 ataagcaacg aacgattttt taacaaccac gttatatctg tgaggtaaat atatgactct
     6001 gagtcaatcg caacaaaaat ggctgttctt catcggactg gccgtcttct tactgttaat
     6061 gtcctcgccc gcgctcgcgt cgcaagggca gggtggcggt ctgccctatg aaagctggct
     6121 gaacaatctc cgccagtccg ttaccggccc ggtggctttc gccctgtcca tcatcggcat
     6181 tgtcgttgcc ggtggcgtac tgatcttcgg cggcgaactg aacgcgtttt tccgcacttt
     6241 gattttcctt gtgttggtaa tggctttgct ggtaggagcg caaaacatga tgtcctcttt
     6301 cttcggcggc gcggaagtta ccgctgccgt gcaggccgca cagcattacc ttcttccctt
     6361 ggcgtcgaaa ggataaaacc atgagagcca tccgcacgat acccatccgc agagtggcaa
     6421 accgtcccaa cctcttttgg ggtggagatc gcgagatggt tcaggtatcc cttttgatta
     6481 cttttgtttt agtggtggcg gcgcaaaaca cctatgcttt cgtaatcggc ttggtatttt
     6541 ggttttttgc gctttacgca ctccggaaac tggctaaata cgatcctttg ctgcgttttg
     6601 tcggcctgcg gtcgattacc aagtacaaac gtttttatcc gccccactcc acaccgttta
     6661 gaaacaatac ttcaacccaa ggaaaccgtt acaaatgatc attgggatta tctcggccat
     6721 atcggttttg ggcttggttc tgtgtacggg tctgttcctg aaaatccgcg ccgttaccaa
     6781 tgccaccaaa ctcaaacagc accgctcgaa agaagaaggg ctggccgatt tgctgaacta
     6841 tgcggccgta atcgatgacg gcgtaatctt agggaaaaat ggctccctga tggctgcatg
     6901 gatttatcgc ggcaaagaca tgggcagcag cacggtagcg gagcgcgaag acgtatcttt
     6961 acgcatcaat caggcgtttg tcggcttggg cagcggctgg atggtgcacg tcgatgccgc
     7021 acgccgtccc gcaccaaact ataccgaggc aggcaggaac tactttgaca gttacgttac
     7081 agaggccatc gataacgaaa gacggcaggc atttgaggcc atcggcaccc tgtatgaagg
     7141 tttcttcgtc attgtcgcta cctatttccc gcccttactg gcagaaaaaa aggtcgccga
     7201 actgatgttt gatgacgaca acgccaagga aggtaaacgc gcccagcaag accgcacact
     7261 ctctcatttc aaacaggctt gtgcgaatct ggaatccaac ctgagcattt ccttggagtt
     7321 gcaccgtctt tgcgcccata aagtcgtttt ggaagacggc atggaaatta cccaagacga
     7381 tttcctgcgc tggctgcatt tctgcattac cggcaaaaac catccgatca accttccggc
     7441 cgcgcccatg tacattgaca gcctgattgg tgggcaggaa ctctacaccg gcgtcgtgcc
     7501 gatgatcggc gataagttta tccaagtcgt cgcgctggac ggcttcccga tggagtccta
     7561 cccgggcatt ttgacgcggc tcggagaaat gcctatcgag taccggtggt cgaaccgctt
     7621 catcttcatg gatcagcatc aggcggttgc cgatttggag aaataccgca aaaaatggaa
     7681 acagaaagta cgcggtttct tcgatcaggt gttcaacacc cattccggct ccatagacca
     7741 agacgctcag gcaatggtgg atgacgcgga agccgccttg gccgaaacca aatccggctt
     7801 ggtggcacag ggttactaca caagcgttgt cattttgatg cacgaagacc gggctttctt
     7861 ggaaagcatg gcaaggcagg tggaaaaaga gatcaacact ttgggcttca atgcccgcgt
     7921 tgagacggtc aatacgatgg aagcatggtt gggcagcctg cccggccacg gaatcgaaaa
     7981 cgtccgccgt cctttactga atacgctgaa cttcgccgac atgattccga ccagcagcat
     8041 ttggacgggc ttggaggaaa acccctgcca gttctatccg cccaattcgc cgccgctgat
     8101 gcaggttgtt accagtggct ccagcccgtt ccgtctcggt ctgcacgtcg gcgacgtcgg
     8161 ccacactttg gttttcgggc cgactggcgc aggtaaatct accctgttgg ccactttggc
     8221 cgctcaggcc gaccgttacc ggaacatgcg ggtattttct ttcgacaaag gccgttcttt
     8281 ggaaacgctg acccgcgccg tcggaggatc acactacaac attggcgatg aagacaacac
     8341 ggcttccggt ttgtctttcg cgcccatgca gcttatccaa aacacgtccg accgcgcttg
     8401 ggctttgtct tgggtggaag cggttatgga attgaacggc ctcaaggtta cgccccaaca
     8461 gcgtaacgaa attgaaaccg ccatcaaaaa caatgcagcc gaacagtcct attcgctttc
     8521 cgatctttca aacaccctgc aagacgtaga gatgcgcgaa gtattgaaag cctacaccat
     8581 cgacggctcg atgggttatc tgctcgatgc cgaacaggac agcttgagct tctcccgctt
     8641 cacctgcttt gagatcgaag agttaatgaa cctgccggaa aaatatgcgc ttccgattat
     8701 gctgtatctg ttccgcagaa tcgaaaaagc cctgaccggc gatcccgcag tcctgttcct
     8761 tgacgaagca tggatcatgt tggggcatcc tgtcttccgg gaaaaaatcc gcgaatggct
     8821 gaaagtgctg cgtaagaaaa actgccttgt tctgctcgcg acccaaagcc tcagtgatgc
     8881 ggccaactcc ggcatcctcg atgtcctgaa cgaatcctgt ccgaccaaaa tctacctgcc
     8941 caacgcccac gctagggaag aggacgcttc ggaactgtac aggcgcatgg gcttgaatgc
     9001 gcggcagatt gagattttgg ccaccgccat tcccaagcgg cagtattact acacctcgcc
     9061 ttacggccgc aggctgtttg aactggctct tgacccgctg accttggcct ttgtcgccgt
     9121 atccgacccc gacaccctga atgcaatgcg gcagacggcc gaccaatacg gggaagactg
     9181 gccgatccgc tggctgcaaa accggggaat caatatccat aactatgtcg atgtggacaa
     9241 atacagaagg aaacactgat gggactgaaa gacatttttc gcgtcaggga aaaagccgac
     9301 cgcgaaatga ccgaaaaccc ctatttgaac gccaggaaac aatggaactt catcatgggc
     9361 gacatggttt cgtcgcgcct gctgtggcaa tgggcgggca tgaccggcct gctgattggt
     9421 ttgggcgggg tagggggcat gatttacctc ggcagccagt cgaaatttat cccttatatc
     9481 gtcgaagtga acaaattggg cgaaaccgtg gccgtggcca gagccgacca gcctcaggca
     9541 accgatgagc ggattgtccg cgccatgctc ggcagcttca tcggcaatgc ccgtaccgta
     9601 accaccgata tagccctgca acgcaaatac gttctcaata cctatgccat gctgaatccg
     9661 aaagacgcgg ccgcagccaa aatgaacgaa tggctgaacg gaacgccgga gtcttcgccg
     9721 ttcaaacgcg cggaaaaaga actggtcagc gtggaaatca agtcggtgct gagacaaagc
     9781 gacaactctt ggcaggtgga ctgggtggaa accactcgcg cccatgacgg caaattactc
     9841 aaaccgcctc agaatatgcg tgcgctgttg aatatctact acgttccgcc gacaacagag
     9901 cagcagatta ttgtgaaccc catcggcatg tttatccgtg atttctcttg gtctaacgaa
     9961 ctgtaacaag gtacagcaat gaaaagacag attttatcct tggcgttaat caccgctttg
    10021 tccacttccg cctttgccga cagctacgcc gacaaatatt ttgaaggcag ccacaacccg
    10081 aagctgaacc gccacgaaca aaaaggcttg ggcatcgcca aaaactactc ttctgacggc
    10141 aggatggccg taccgcccgt tcccggcgat aacggccgca tcacctttgc cttcggctct
    10201 gatccgtctg tcgtctgtgc cgtaatgcag gtctgcgaca ttgccttaca gcccggcgaa
    10261 atgattaacg atctgcacgc cggggatacc gcacgctgga atatcactcc ggcaatttcc
    10321 ggcagcaacg gcaacgaaag aatgcacatc ttggttaaac cactggatgt cggtttgcaa
    10381 acgacgcttt tcatagccac cgaccgcaga acctatcata tccgcctcaa atcacaccgt
    10441 acccgttata tgcctgccgt cggtttttcg tatcccgaag acaccgccgc gcgttggcgg
    10501 gcgatccggg caaaccaagc ccggcaagcc gaagctcagg ttctgcccac cggagaaaac
    10561 atccaaaact tggatttcaa ctacaccatc agcggccaca atccgtcatg gaaacctttg
    10621 cgcgtttata acgacggcac ccgcaccatc atccaaatgc cgccgacttt atcccaaggg
    10681 gaagctccgg ccttgctggt cgtccgtaac ggcgaaaaca ccttggtcaa ttaccggctt
    10741 cacgaagacc gctacatcgt tgacagcctg tttgacgaag ccatcatgat caccggcgtt
    10801 ggccgccatc aaaccaaagt cgttatcaga cggagccgat catgaaatca aatgtcctgt
    10861 tcgtcgcgct ggctttggcc gttggcggat gcgccaccgg gcaatacggt tctttcgtcc
    10921 gttcagacgc cgtatccgcc tcagtaactc aagatgtagt ggaaaaactc caagaagaat
    10981 ttccggcggc caacaccgtt ttcttcctga accgcagcaa atccgatacc ttcggtacgc
    11041 aactggatca ggcattgcgt gaaaaaggct ttgccacaac cgcccaacag ggcgaaaacg
    11101 aaaaatcact gaattacgtc ttggatcagg ttgattccga tatttaccgg gtaatgatct
    11161 acattgacgg cacgccctat tcccgtgcct accgccaaac cgaaaacggc gccgtggttc
    11221 cgaccggttc ttggactaaa cgtggagact gaagcatgag ccaaagcacc gaagatctgc
    11281 tgaacgcatc ccgttcccct tcgatcgcac cggatacttc cggccgtcgt cccggtgtgc
    11341 gccgcctgaa caacattccg atctatatcg tcggcggtgt tgttctcgga ttcgccctgc
    11401 tgattgcact ggtggcagca actaaaggcc aacagtctgc cgggcaggca acgcagggag
    11461 acggtaagga tgcaacaaga atggccgatt ccctattgaa cgaactgcct caggaaggcg
    11521 gtctgattac cgccaaagca tccgaaccgg ccatgcccgc cctgcctgac ggcatggcgt
    11581 ccgcgcctga tttaccggca tccgcgccca catctttacc gatggttccc gatatggcca
    11641 accttccggc cacacctccc gaagtggatc aggaagcgga acaaatccgg caggcaaaag
    11701 cgcagatgtt ccaagcagcc ctagctgccc caaccaaagt ggatgtcgtc gcatcgactt
    11761 ctcggggttc gtccggccta tatactgctg ccttatcttc ttccggctca ggtacaccca
    11821 accgtagcga gatgctggct caaatcgccg aagtgcagcg catgtccgag aatgccggtt
    11881 ccaatcccgc cgaagtggag cgcgtttaca accaacaact ggccaatgcc caacagatgg
    11941 ccgccgcagg tggtcaggac ggcgtacaag gggcaggcgg catgcccggc ctgaacacac
    12001 ctccgactcc cggcaatatc ccgagacaaa cggcacaaaa cggtggaaac aactggcggc
    12061 ttgagagcca agtcgaacgt ccgaacgcct acatgatccg cagcggtgcg gttatccctg
    12121 caaccctgat cagcggcatc aattccgatc tgcccggcat ggttcaggca cagatctcac
    12181 aaaacgtgta tgacaccgcc accggccgtc atctgctttt accccaagga tcgcgcttgg
    12241 tcggcactta cagcagcggc atcaatcttg gccagcgtcg cctgtttatc gcgtggaacc
    12301 gcattatttt ccctgacggt aaagccttgg acatcggcga tatgcccgga gccagcggcg
    12361 caggctacgc cggattcagc gaccgcatca acaaccatta cggccgtctg tggggcaatg
    12421 ccctgatgct ctccctagtc ggagccagca tcacttacgg cgtggacaaa aacagtaaca
    12481 acaataacaa cggcaataac aacggcaaca actccaccgt gcgcggagcc ttgtcggagt
    12541 ccttgggtca gaccttcgga caggccgtgt cccaaagtat tcagaaaaac atgaatgttt
    12601 ccccgacgtt agagatccgc ccggggtacc gcttcaatgt cattcttact aaagacattg
    12661 atttctctaa gccctatcgt tcctttgact attaacagga gagcattatg caaaaacgtc
    12721 ttttaagcaa aaccaagacc gtagccctgt ctttggccgt ttccgtgtcc ttggggctgt
    12781 ccgcaccagc atacgcaggc atcccggtaa cggatggcgg caacattgcc caaagttcgg
    12841 tctccgccat tcaaaatgtg gcgcaaaccc tgaagcagat tgaggagtac cgaatccaac
    12901 tgaaacagta cgaaacccag ttgcggaact ccttggctcc ggcggcttat gtttgggatc
    12961 aggccaacaa aactatggcc aacatgaagg ccgctgtcgc ccgcttgcga gcctacaaac
    13021 agcaactggg taacatggaa gagtacctga gcaaattcca agccgtggac ttctacaaaa
    13081 gctctccgtg ctttaccgcc aaaggttgct ctgcggccga ccgcgccaaa ctcgagcaaa
    13141 actacgccca attgtcgcgc atgcagaaat tatctaatga cggcctatta aggagcatca
    13201 ataacgagat cgactccatc agcagagacg ccaacacctt aacccaactc caacgaaacg
    13261 cccaatcagc caccggccag ttggaggcac tacaatacgc gaaccagttc catagcgctc
    13321 aagcgcatca actgcttcag ttgcgtaata tgattaatac tgagatgcag gcaaatgctg
    13381 cttatcgtca aagtcaactt gatagagagg ctagatatga agctgctcgt aaacaagtta
    13441 ggacgtcgga tattttgaaa cacccttata gaagagggca ataaaaatag ggtttctaat
    13501 gtgtaataaa cattagaaac ccactctaaa tttgaaagat tacctcatga gaactttatt
    13561 ttttgtattg ctaagtagtt tcatgcttgt tgcgtgccaa caaaaggatg agaaccaaca
    13621 acgcagagaa gaactacgta aagtagatca ggatgtccta aaacggccat acaaaaggaa
    13681 agagtagtta ttttcttatg caatatataa cccaaattat gaaaaaaaaa tatatttatt
    13741 attttatatt ttttataacg ctctctatgc caattatctc tcatgcagca ggtcaaggag
    13801 gagttttaga tgaggcatta tcacgctacg aaggtaaaat cggagcgtgg gaagcagcat
    13861 tttataaagc tggattattt atattttggt cattatcttc aattagtatt gtaatgactg
    13921 gagcccaact gattttccag agagataata tcagcagttt ttttgctgaa tttactcgtt
    13981 taattttatt cttaggattt ttcctttgga ttatcacaaa cggaattaaa attacgacat
    14041 caattaaaga tggtttaatg aaacttgcag gacaagctag tagaaatggt gagcagatta
    14101 cacctagtag tattgttgac ataggtttta atttatttga caaaattgtt gaaaatagct
    14161 caatctggga tccaattgat agtgccactc ttattgttat tggtttagtt attttactaa
    14221 tcttagctgc tgtcggaatt aacatgttat taatgttaat aacaacctat attgtcatct
    14281 attcaggaat aattatcttg ggattcggcg gtggacgttg gacatcagat atggctgtcg
    14341 gatatttaaa acaagtatta aacttatctt tacaattagc agctatgatt ctaattattg
    14401 gaataggtca atcgattgtt caagacacta taaacactat aggaacccct ggatttagag
    14461 aactagcagt tgttctagtt caaacagctt tacttgttgg tttagtcgtt aaaattcctc
    14521 caatgatagg ttccctagca ggaggagcag gaagtggagg aattggctcc tttggtgtag
    14581 gtaatgctat tgctgctgct gctatgttgg caggtgctgc tgccggagca ggtgcggcat
    14641 taaaaagctt agccgtagaa gctggtggtt taaagaaagc atttgaagct gcaaaagcag
    14701 gcttgggagg ttcaaatggc tcatcagatt caggtagtgg gaagatggat agcgcagttg
    14761 atgccgttgc aggtgcaaat gccggagata aatctaacca agatggaaaa ccactgactg
    14821 ctgagtccag tggtaactca gaaaatggta gtgaaggatc tactacagga aattcgggag
    14881 gtaatccatc aggctcctcc gaagaaaata caatttcatc cggaagctcc tcaagttcgg
    14941 gggaagcaaa ttcaacaaat acatccgctt catcaggagc ggaaggtagt gccgaagggg
    15001 aggctacttc atcagagaca tctcaatcag cttctgattc gccaagcaat aatagcggag
    15061 aggcgactca aacggctgac accacaccaa aaacaactaa taccggtgga ggaagtcctc
    15121 ttgctactgc gatgggtggt aataaagtat caaattggca ggcggcaaaa aacgttgctg
    15181 tcggccttgt tagcagcaaa tggaacaatg ccgtcaataa ctctgccggt ggccgcctag
    15241 tcaaacactg ggaggacgaa aagaaaaaaa cagaagaccc cacaaaaaat ccccaaaaaa
    15301 accctgaagg agatgacaat gcttaaaaaa acgcttatct gcatattgct ggtcggttcc
    15361 gtacactttg ctatggccga aagcctcaac gatgcgctga accgcagccg ttcgggaaac
    15421 tcgaacaaca acaggccgca ggttgaagac ttgttcaccg gtgatgtaaa gctggcctgc
    15481 gaagccacgt tatgcctgtc cacgggcaac cggccgagtg aatgccaacc ggccatccac
    15541 cgttatttct ctatccgcca caaacggatg ggagacacaa ttcgcgcccg acacaacttc
    15601 ctgcgtttat gcccggcatc taaagagcaa ggcatgctcg aactggttaa tgccattgcc
    15661 aacggtgccg gtcgatgtga tgcccgggaa ctgaaccgta tcaaccgttc cacttatatc
    15721 aagcgtgttt gggtcggatc aagacggcat ggagactggc aagaggtcaa agtcccctat
    15781 atccgtaacg ccaaaccttc gtactgctct gcctactttg aaaacggctg gacacagctt
    15841 ggcacacgct atgtaggtga agagaaaaat ggcgggaaat gggttgacta atggtttatg
    15901 tggccgacct gcccccattt atgcaggaac gtgttacctg ctcgattgct gcggctgaaa
    15961 aataccatct gccgccacat tacctgttgg ccgtggccga acgcgaaaac ggtcgtcccg
    16021 gtcaacaggt acgcaatacc aacggcacct atgatgtagg cgcgatgcag attaataccg
    16081 cctatatccg caccctatcc cgttacggtg ttactcagtc agccgttgcc caacccggat
    16141 gctatgccta ttacttggcc gcatggcgga tcaagcggca catcgtttat gatagaaagg
    16201 gagatatttg gacaaagatc gcaaactacc attcctatac gcctaaatac aacgcaatct
    16261 atcgcgcagc catcattcgg ttggccgaaa aatgggaacg gtggcttgcc ggtaggcaag
    16321 ggaataacgc caagggaaat gttcccacac aagctgttcc agccgttcgg acaattcggt
    16381 cgcaaccatt gcccaagcaa tatgtttccc cgacgaccgt ctatgttcct agaacaatcc
    16441 gtgtggtaga gcgttccccg gagccagagc cggaaaccgt ccaaaactaa cagtaaagac
    16501 catctgaaaa agagtttcag acggtctttt ataatttccc cttgacatgg atggtaaatt
    16561 catgtttata tcaattcaat tatttaggag gtatggaaat ggcttacgca attgcatttg
    16621 atttggatac gaccgtttta caagaacttt accctaatag tagctggcaa aatgcttacg
    16681 gtgatgtaaa aagaaccttg gtcggattag gttttaacca tcagcaaggc agtgtatatt
    16741 ttggaaactc tgagattaca gccgttagct gtgtcttggc cgcccaaaaa ctgagccaga
    16801 cctatgcatg gtttaagccg gccgtttcgg atattcgtat gttgcggatt gaggaaatga
    16861 acgatttgca gcccgcttta taaagcgata ggtatcttag aaaagggcag cgcggtgtcc
    16921 ccgccctgcc ctttcccttt ccttccagcg ttgccgagta aaggctgcct agtttctact
    16981 aggcagccca aactactttg ccttgcaagc ccgtttaacc gtactttcgc taacattgaa
    17041 atgtgcggcc gtccgggaaa tactggtttg ttcagactgc cgccatcggc ggatttcttc
    17101 gggatcgact ttcactttcc gccccagttt tttcccttct tccctagcat tgcggatacc
    17161 ggccatttga cgctctttga tgtaactgcg ttccaactcc gcaaatgcgc tgacaatagc
    17221 cagcatcgcc gtacctaacg gactggccaa atcgaagttt tcccggagcg aggttacggc
    17281 cacgcccttt ttctgcaaaa actgcaccgt ctccaaaaca tcgatggtat ttctgcccaa
    17341 acggtcaatg gctactacga ctacggtatc cccttggcgt acaaattcag ccatcttacg
    17401 gaactcaggc cggttaaggg cttttactgc acccgaagcg gcctgatctt cataccaccg
    17461 ttcgatctgg tatttgtctt gaataacctg tctttgtgcg gccacattct gctctttggt
    17521 cgataccctc aaatagccga taatcataat tcctcaaacg gattaaaaac tcttgcgccc
    17581 aagtgtgaaa aatccttttc attgcgcgtt accaccatca tatgctcctg tccaacggtc
    17641 gtcttcgata agcacacggt caaactcaat gtctgcaata tctacctctt ccggaggaac
    17701 ggccaaggca tcagcgatac tctccccctt acccaacgat tctgctccta acgaaaggtc
    17761 atggggacag ccattcaaaa acggagacaa ccgtacctta taagccggaa aatcggtaac
    17821 aaactttctc agtttttcat caaccatcct ttgaacaaat ggattgcctg tcgctgcctc
    17881 gatgttgtgc aaaatgtcgg ccaaatctcc gattatcgtc aaattatcca gcgcagccgc
    17941 ataactctca ggccgatacc cgcgccggga ataagacagt tccctgataa tcagcgaaat
    18001 ttccgtgatc aaccgcattt tttctttcat ttccgtatcc ctgcttatct gctgatggat
    18061 tttttggcgg aatactccaa agacttgaca atcaatttgc ccttattgct tccgatagaa
    18121 acgttctgcc ctatctcaac caatttggcc agtctttctt cgttatgtgt tgcattgtgc
    18181 ctgacaagcc cgtttttggt tttttgaaat accgcagcgg catccttgta aatgatttct
    18241 ccggaataag tttgaccagt ctctgctttt ttagcttgac ctagtaaata gataccggct
    18301 ttcatgcctg ttacaggaat ttgttccacc ttagaccaca actcgtttgc tgactttgtt
    18361 tctagggtaa cacccttgga gttagtaact accctttgct gggaatactc ttttttggcg
    18421 aaaacacttt caagatccat actgcccttg gcctgattga agcattcccg gctgattttt
    18481 cctgagtcga agtcgctctg gattttctcg gccaacctgc ccaatatttg ctcttgcgac
    18541 cccaaggaaa gcattttgat gacatcatcg aacttgtcga taaattcact gttcctgtct
    18601 tggttgatac cgacaatttt taccgcatca ccaaacttat ccctgatttg cttgagtccg
    18661 tcaggaagat tggcctgaat atcagccata atacctatac tggctcccct gccgtattcg
    18721 ttaaaccgtt tgtaagtatt gtcggaagcc ctttcagccc tcatgctgac ggctacgata
    18781 gtaacttcaa gattacgctc cagacactgc tcaatcttgc gaaaagcaga ctgcggccgt
    18841 gcaagctgtc cttcaaaaag cagcttggtg gtatcctgca tcatcatgtt ttttactgtc
    18901 gaagtttttc ccgaacccgg aattcccgta acgaaaataa cttctttctt ctgtggctcg
    18961 tcgcgtttca gtacctcgtc aaactgggta gaagataaaa ctgctgctga attgtggatg
    19021 gcattattaa ccgttgccct gtcttctttg ttctcaaaag ctggaaacaa ctctttaaac
    19081 gtatcagcac aaacatatct accgccttct gtatctgcat gttggctata taaagacaat
    19141 atctcgtctg tttgttcggt cgcaaaacgg atggcctcat cctgaacgga agacaaatat
    19201 tcgttattgc caaaatctct cagattaata tcgctagaca gttttaccat aacggattat
    19261 tgctccttat ttgccgccca caacactctt aacttagcta gaagctcagg agatgggccg
    19321 gcatacaggc gtttttccac ttctttgcgg aaggcttcgt ctgtcatcat cctgcggttt
    19381 ttttcgcaag cctcttttat cattttgata gcattacttt cttggggctc aactttattc
    19441 ataataaaat cctaatctta attttatatt actcattatc gcttcatcgt tttctttacg
    19501 gaagcgtccg atgctttgac ggacaaccca tttccttccc gcttgatgga aactttctga
    19561 ccaatttcca cctgttcgga cagactggag aatttcccct ccgacattcc ctgaaccgcc
    19621 ttatggcgta tcagaccttg gtctgtcttc tgaaaaactg atgcctcgtc tttatggaca
    19681 atctctccac tgtactcttt gccggattcg gccggtttcg ccgtgccaag catgtgaata
    19741 ccggctttca tccccttggc ttcagccttc tcaatatcct gccatgtgtc gcctgatttg
    19801 cgttgcagtg ttgccccttc tgaattggcc accacccttt cctgacgata aatctcttcc
    19861 acacgctgat agatctcttc ccggtgttgc tttaccttgt caaaataatt tggaacagga
    19921 tcttgggaac gcctgtctgc ctgaagctgt atcacatccc agacttgagc aacctgcttc
    19981 aaacgttcag gagtaatctg ctgataaaac tccatagcat ctttggcact cgcctgtttg
    20041 ttctgttgtt tattcaggct gtcatacagg atttccccgg ggcgattggt tacgcggatg
    20101 cggtcgaatt ccgcctcatg agttgcaact gtatttttaa atccctcaaa cgccttattg
    20161 tgaaaatctt cgtcaacgaa acgcggttgg atattcttag taagatactg atcttcatac
    20221 cgtttaaaaa taccggccaa actttcttcc ggagcagtcg caacggccaa catttcgatt
    20281 ttcagtcccg cttctcgcgc ggctttaatg ctcatgccta ctgcttcagc attacggaaa
    20341 gttccttctt ccaaaacgtt ccgggagttt tccaatgccg attttcgtac tcctacagcc
    20401 aacttgcctg catcttcttg tgtctgctga gacgaataaa cgactccagg aggaactggt
    20461 attttttgcc gcataatgtc ggcatcaaca tgtatatgcc ctccctttga agcgaactct
    20521 tcttttacga tgtcggcggc cttacttttt cccgctcccg gttgagccgc taccaaggcg
    20581 atctgcggca catctaccgc ctgtgtaatt tccaaaagag cagacatata ttcggcaaca
    20641 atttcggcgc gtcccgcgtc gcttaacttt tggtaatcac tcatacagaa cccccaacaa
    20701 tcgtatcatt gggatccaaa atccttgcga tgacatcttc gtccgttgct cttaagttat
    20761 ggcggaatac tgtaatcgct ttaatactat ccgaacaata ttgaatgaat gcttcatttg
    20821 gatgagattt tttccgctcc tcttctctag cctgaataaa cggttgggcg gcattaccca
    20881 aaacctctaa cgctacttcg taattgatac ccggaattac ttctttcatt ttttggttcc
    20941 ctccaaaaat tatccacaaa ttttgttagt aagctgccca tatcgccagc ctgccacaca
    21001 taataaattg aatcttcgtc atcaaaccgt ttttgcctgc tttttaagca acatggttca
    21061 aaacctatgt ttgaataata agccagttca aaataaataa caacgttttt gacctatact
    21121 tccagacctt aacgaaacat agttcataaa gtacactttt tgcacccact tatctaggtt
    21181 actcacctct tactcaagat ttggccattg cccaccatac cccataatgg ctttaattga
    21241 ctcctgtcaa gcccaaagac tctctttcat gtttataatt ataggataat ttatcctact
    21301 tgtcaaataa attttagccg ctaaattttt actctcgctt atttattcat tataataaat
    21361 taattgttat tctgcttaac tgtaaaaatt aactgtgagt gattggagac accatgccac
    21421 atgagcaaat gattgatgct ttttcagcaa tcattcaaaa ctccttaagc ctatatgaag
    21481 agcaaactcc gctatactac tacctgacta tctgcggttc ttgggttctg actgcactcg
    21541 gcatcctctc cttggcgtcc gtattcttta ccatcaagca gaaaaaacca taccgtttta
    21601 tcgtcccgat tctgttttta tggctgggat atgaaagcat ccacatgccg cgcccgaatc
    21661 ttgctgtatt ctacggatac aacttggcgc agctttccta tccggcaaac gaaaatccca
    21721 actcgttcct acatctcgca gacggctcaa atccgatttg ctgcttcata ggcgaagacg
    21781 agtatttgca agccaaacgg ttaggtttca aagagtatgc cgatgcaaaa gcaaaggctt
    21841 tggttgattt ggttgaaatg gagaaaaaat ccaaacaatc tgattgaatc ttaaattcaa
    21901 taataacgcg gtagggggct ggtcaagcga aggttcaggc tctgactgaa atttcgcttg
    21961 gacagggggt gtggggcgga gcgcagcgaa gggctaccgc gtggcggccg aagccgcccg
    22021 acaaactcca gtttgtcgct attccgtttt cagtgcgtaa atacctttcc ccactctcag
    22081 aaaaaaccgc tcgtcctgta atacctgcct gactttggcc tgccagttcc tgttttctgc
    22141 ccgcgcagcg gcatacggtt ccatttcgcc ataaatttcc gtcaagccca tattcctccc
    22201 gtctgccgcc tgcaataccc gtcttacggc ggctttccat gtaacggccg ttaccgcctc
    22261 aaccctattc atcgctttag ccaaaaagta caaggccgtc tgaatctctt ttttaaagac
    22321 cagcagtttc tcatgggcaa tccgtaccag ctttccgcca tattggcggc tgtcgctgac
    22381 gcagttgtgc tggattttga taatctcatc caccagccgc cccgccgcca tccgttcaac
    22441 catgctgctc aaattgtagt aacggccgtc cttcctcatg ttgcccatta gaatcccata
    22501 atgcccgccc tgttcggtcg catcatggat gttcatcatg gccagcagca tagcctcttg
    22561 aaactgttcc aagtccattt ggctcaaatc ccacggattg gctgcatcac cccattggtt
    22621 gccactgtat cgaatcatgt tccaataagg cggatgccac caaattaaat tggcctgttc
    22681 tcctaaaaat gccgcaaaat cctgagtcag caggttatat ccctgatgca aatccgttcc
    22741 tttaaaacga atgcccattt cctccgcaac atccgaactg gtaccgctgc cgatgctcgg
    22801 gtcggcaaac agtccttccg gagcaagata gctttccaca aagtcgcgga tgatatgccc
    22861 cgtgcaattt ccgcgataac cgcttttacc gtaatttccg cgctgcggat aagaacaaac
    22921 agaatgcgta aacatggttc attcctcctt taaatcgtct tcattttcgc gataatggca
    22981 acctaatact gcatccaaat cccgcatggt ttccaccggc tcgtcattga cataaaagtg
    23041 tgtatccagc gcattgatta aaaaacccgt ccgcaagaac ggccaccggt tgcggatggt
    23101 atcctgcaaa cgctccagca aatgatgctg ccgaaggttt ccaaaatgcg gaacggtttc
    23161 atatgtccgt gccgtttccc aaatgtcctg ctcgaagttg ataaacgagt cgccgccgag
    23221 ttggcgcagc agattcatcc aaccatcgaa actgtcggac acatcatccc aataaagcat
    23281 aatttcctcc ttatcactca atccctgcat cggccaaaat caaagccaag atgccgctgc
    23341 gccgtgcttt ggcagttttc agtttcagac actgcaaatc atcgcaaaca atctgatacc
    23401 cgcgcgtctc cagttcggcc gcttctgaca cttgcaggct gtaatactgc ccgccatcga
    23461 atgtgtatcg gcaaagcagt gtaggggcat ttcgtccgtc ccacataacg ttaatcggac
    23521 tgaatcccat caactcccca ttgctccaaa tattctgctc gacaaataca ttcggatgcc
    23581 cagttaatct gttggtatag cttgccatct ctgtctcccg tactttcctt ggtgttaaac
    23641 ggtttttgaa ctgaaccagc atcggcgttt ccgccccgct gtgatgacgt taaaagacaa
    23701 actgctttga gatacaaggc tgccgcaggc accgcaggct cggccttgta tctcaaagca
    23761 gtttgtctta ggaatcacgc aacagcgggg cgggaatgcc ggttgcgtcg aacggccaaa
    23821 agccgtttga caccaaggaa aaccttctgc cggccgcagc cggctcattg cccctcacgg
    23881 ggaaggggtg gggttcggag aggcggtgcc gcagcacggc cgatacttaa accaagagcg
    23941 gtttgatgcc ttagcatgaa accgctcttt ccgttcaatc ctgctgactc ttgattagcc
    24001 tgaaactccc agcaccttca agataaccgt ttatagtctt gcgaatggta acatattccg
    24061 tccccatcat tccgaatccg cccttagtcc aaaaaaacgg ctccctcggc aaaaaaggaa
    24121 tatattcctc ctcttgaggc tcgcccatgc caagactgat ctcgggctga ttcccatccg
    24181 tctcataatg cagcttttcg tcccctttga aaaaatcggc gacgaccgct tccgcctctt
    24241 caagagattg gctgataaat aaagccgaac taccaaagcc gatctcacct gtctctacaa
    24301 ggtagaaagt tcgattttca gcggacagac tcattttctc cactcccgat aaacgcctat
    24361 gtcgttatgg ataaaggttg gatagatacc gatcccacct accctgaacc ttcttgccaa
    24421 agcggcaagc tcggaaaggg acgctctttc gatatgaaag tcccccgcat tgccggacaa
    24481 atgttgggag tttttggccg ccccctctaa cgagtcgtta tgtcggcgcg tgcggaatcc
    24541 gctatgcaaa accaactccg gattcggtct gcccaaatca cgcaaatact gctgctgggc
    24601 aaacagcaga ttgatcaaat tgatgttgat ggcgtgcatc tgatggttgt ccaccacatc
    24661 ccgcatcatc caacacagag ccttatagcc ctcttggata tactggccgt cggcaaagaa
    24721 tttgatgttt ttccgttccc gggaagcagg ccggtaaatg gacaataccc ggtttcgttt
    24781 ccaaaaccgg ctgtcatccg ccaaaacgat ttccggcaac agtaaaaggc cgcctgatga
    24841 agcaagcgcg gccgctttgg ccgacagctt taaaaaatcc cgacgattca acgctccctc
    24901 cctttcacgt taccgctgac cataaaagca taccggccgt attcgttacc tccctcattc
    24961 gccgtttctc cggccgcaag ggatcccaaa acctgctcta atcccatttc gaccgcaggt
    25021 ataaaatctt catctgtcgc cgtaatggta atgctgagcg tcttggtatc ggcatgcgta
    25081 tctacccgat gattcagtcc gtactgcttc tgaaatgcct tgacggcggc ctgttcggac
    25141 tgattccgtt cggccgacac gcccattttt tcctcttcgt gccgctgcaa catttccaaa
    25201 taatcgtcaa acgccaaggg ctggttctgg ctggttttcc ggtaatcgac ggcgatattt
    25261 tgcagcgtct gccgcaggcg gccgtcaaag gccatgccga aaacccgcca gaaccatcct
    25321 tccctggcct gtttgtgata agtcagaaaa aaagccactc tgtataattt gtccttatcc
    25381 ataacacccc ctatcggctg ccgcgcgatg cggttttccg ggttgttgcc cgctgctgcc
    25441 gcttggtctg ttcgacaggc cgatcctgag acgcttcttc ctgcaaaccg cgtgttttca
    25501 gggtttgcaa ttcgtttgcc ggtcgggcat gtatcgcttc gttgatttgc ttggccaaac
    25561 cttcgcggcc gaacttggat ttctgagcca aatcgttgaa atcggtaaat gccttatgct
    25621 ccgacgactg ctctcccggt gcaaacactg gaaacactgc acggcagtta aggatttttg
    25681 ccgcctccaa cgctttttct ttgccgggat tgttgccctg cttggcttcg acagccaaat
    25741 catcgtctcc ggcgaccaaa aactgagcct gagggtattt cgcagacaag actttggcca
    25801 ccaaaggcag gttgccggaa ttgaacgcgg ccacactggc caccgtatcg tcggttgctt
    25861 ctttaatcga ggcggccgta gcatagcctt ccgataaaat aatggttttg gccttagcca
    25921 gatcctgttg gccgacaacg tgcatacaac cctcctgctc gctgtctttg gcaaaacgtt
    25981 tggtaccgtc cgcctgaatg tattgcatgg agcgtaactc cccggctgcg ttgtacacgg
    26041 gaatgaaagt tttctgcccc tcgcggtcgg taaagatacc cggctgcggc ttgataccct
    26101 tggcattcag ataaggcgtg cctgtttgaa cgggcaggta gctttccata tagtatttga
    26161 tacggccgac agcagcttcc tgagcctgtt ttttctgctc gctccgcatt tgcagcttct
    26221 gagcggcctc ggcggccagc ttggcttttt cctccggaga caggctgtat ccctttgcct
    26281 tccacttaac ctccgctccc gtccggttgt ttttgatgta gcctgccggg tggccgtcca
    26341 aatgaccaac ataaaagccg ctgcgctcgc cttttttgtc gccgacggtt tcgattcggt
    26401 gtttttgtcc gtccataaac ggatgcccat tagccaccac acaccccatt tcgcgcaact
    26461 tgtcggcaaa ctcttccacc ggcgacatcg cgggcatgct ttgagtctgt tgcttttccg
    26521 gcaaccattt tgacgcggcc ggtaaattag cgttactgct ttccataaac caagatttgt
    26581 tggctttgtc ccatttggca ccaagggctt tagcctcttc tttttcttca tacggaacgg
    26641 caagataaac acgggatgcg ttttcttctg ccggtgcttc ttctgttttg gcagcctgat
    26701 actcttgctg tatggctgcg acctgacact caactgcttc ttcctgagga gtatcgtccg
    26761 gcacggcctc ttctgccaac gcttcctgct tcggctgcat ccaccgtgca aacagtgcgg
    26821 tattcacgcc ttcggggata taccaagact tctgatcctt atcccatttc gcgcccaatg
    26881 cccgcgcttc gtttttttca cgaaacggca catcaattag cgttttcgct atggccggag
    26941 tattgtccgt taccgttttt gcttggttgg tggcactgtc cagccatttt ctgaaaggct
    27001 cttgattgac cccgggaggg atgtaccacg atttctcctc cttatcccat ttcgcgccca
    27061 gcatttttgc ctgttccctg tcggcataag gaatttgaac gtaaacgcgc tcatcggaaa
    27121 ccacgcggac gctttccttg tcctgctccg gcagaaactg gcggatctgt tcttcaaccg
    27181 tacgataaga ggactgctcg atggcttcgt ccaaagcctc tttggccgca ttcagatttt
    27241 ccataaatga ctctccattc tccggttttt gatgccgctg ccaaaaagcg ttctgaaacg
    27301 tggcatcatc catctccgaa ataaccagac tgaaacgctt caagccctcg tgcattttct
    27361 cttcagccat gaagttgatg atcgccgcca catcattttt atcaaccgca ttgataaggt
    27421 tcttttcttc cgccaagctg gcttcatcaa tattctgtct atcagaaata ctcactgccg
    27481 cctcgttctg ctgttcgatt tcccgcacct gatcaaaggc catgacatag ccttggattt
    27541 tttcggcgtc agaagccgca cgcatgattt ccatcggatc gtcctgcaat accataatcc
    27601 agcttgctac ataactggca tgttgcccgg gattgaatga aacgcccaat tcctgactca
    27661 gcatcaatga agcgatttcg gcacgcaatt cctcccgtgc gtaaccctcg ctgccgaacg
    27721 ggtgggaaag gtcgcggttc aaacggtcag catgccccgt ccaatgcccc aattcatgca
    27781 gggctgtttc ataatacgct cccggacttt caaactgctc gcgcagcggc agggtaatac
    27841 tgtccctgct cggagagtaa aacgccctgt cgccgtgccg gtggtcgatg accgcaccag
    27901 aatttttcag aatgttttct gcccgctcat ccggattcca acttagcggc cgcagcttca
    27961 acggcggcaa gccgtctatc tgttcgccgt taaataccac cgcatgaaag acctttggac
    28021 gctccaaacg aaccgtttcc ttctccggtt tgccgtcctg gccgataatt tcctgaccct
    28081 tttcatcggt tttgggaact tcctcggtga actgccaata ttcgacaaca gtacccttct
    28141 ctccacggcg cacctgcgcc ccgatggatg atgcctgttt ataggttatc cagcgcggat
    28201 cctttctgcc ttgtgcctgc aaccacagat tgttaatgcc gcgataacgg cggccggtaa
    28261 tggcattgtg cggcataaaa tccggcaacc cgccgccgtc ccaaggtttc tgccacggcg
    28321 cggttccctg ccgcaactgt tcgatcagtt tttccgccac ctgttcgtaa aacggttttt
    28381 tattctgcat ccctacgctc cttccgccac ttcgcgggaa aggtctgcca cggaatcccg
    28441 cgcggtttcc gcatccattg cggtctcctc aaacgccccc aaggcttctg cctctgccgg
    28501 atcaaaatcc acttccattc ccgggttatt caaatccgcc actttttctg cgatcacttc
    28561 gatgtcgata cttatacctt atcctttcgt atgtttggaa tagtccggct taccgccagc
    28621 atcggggtaa tactgcttgc actcggggta attgctgcat ccccaaaaat gcgttccttt
    28681 ctgttttccc ggccgtctga ccaagggatg ggtgcaggcc atgcacttga attttgacga
    28741 cggctcggct tttttcggtg ccagtaccgg ccgaccggcc ttgtcggaaa aagaaacctt
    28801 gcatccgtcc ttatagccgg tacagcccca aaaaaatcct ttcgatccgc gcatccgcct
    28861 taaaggtttg ccacattcgg gacacgggta cttcttgaca ttgagactga tgccttcccg
    28921 ccctacccgc tccacctcgc cggatatgta ctccatcaga ctgttcacaa aacgttgcgc
    28981 gtccaaaccg ccttggacaa tatgctgttg ctgctcatgc cataaggctg tcatatcggg
    29041 gaacttcgcc tgatcgggca gcgcgtcata aagctgttgg ccgatttcgg aactgacaat
    29101 gtttttgcct ttttccagca gaaaaccctt gtccagcaga ttacgcagaa tttcgtcccg
    29161 agtggccggg gtgccgattc cgccgtgctc tccttcctta cccttgtctt tctcaatcag
    29221 cagttcgcgc aatttcggat tctgcacata cttggctacc cgtgttaaat cgttcaacag
    29281 cgtatccatc gtgtataacg ggcgcggttt ggtttcaaac tgcgtctctt ctgctgtcaa
    29341 acaccggccg ctgtttcccg gtttgagcat ctgcaaatca acactcaaat cctcgggatc
    29401 tgtcgcggtt tccgcattgc cctgatcgtt cttgtataga gccatccaac cgggctttac
    29461 ggttacgttg ctgcggcaga caaatttttt accggcaatt tctgcttcaa tgtcggtttg
    29521 gttatattcg tgatccggcc aaaactgggc gatataggcg cgggcaatca gcagatagac
    29581 ccgtttttcg tcttcggtca ggtcgtccat attggccgtt ttgacggtag gaataatggc
    29641 atggtgcgcc gaaactttat ccgggttgaa cgccctgctt ttgattttcg tgtcggccga
    29701 tgcaatggca tttttaaaga ctgatgcggt ttttccgaca gcatccagca cgtctgccgc
    29761 atcatcgaaa tgctcgtcat tgagatactg gcagtcgctg cggtggtagg taatcaaacg
    29821 gtgtttgtct ttcaatgact gtccgatgtc ttttacctga tcgggtttca aaccgaattt
    29881 gcgcgaacaa tccgcctgta gtttgagcag attgtatggc agcggcggac tttgccgctt
    29941 ggcagaagtt ttagccgcta aaatttttac cggctgacca ctggctgctt cggctatctg
    30001 ccgtgcaaac agcggatcgg acaaacgtcc cttatcgtct atcggatctt gatccttgtt
    30061 tggaacgtaa cgggcagtaa accggacacc atccacatcc aaatcggcat acacatggta
    30121 ataatgggtt ttgacatggg actggttttc ccgatcgcgc cgcaccacta aacccaaaat
    30181 cggagtctgt accctgccga cggacaacac tccttgataa ccctgttccg cagccttcag
    30241 cgtataggct cgggtcatgt tgtatccgta taactggtct gccaccgatc gcgcttccgc
    30301 cgattgggac aatcccctga atttggcgtt gtcttctatt gccgataacg ctctctttac
    30361 agatcgctcg ttattgtcgt taatcagtac ccgtttgacc ggtttggcac aattcaaaaa
    30421 ctccaacagt tcgtcaacca acaactgtcc ttcgtcatcg ggatctccgg cgtgaacaat
    30481 ctcggtacat tccgccagca aatcacgaat aaccccgatc tgttcttttt tgtcggacaa
    30541 gggtttttta cgccaaggga tatgcgcgat cggtaaatcc tcaaacttcc agttaccgta
    30601 tttgctgtcg taatcttccg gatcgcacaa agcaagcata tggccgtaac accaagtgat
    30661 gttgtctccg ttactgcaac ggaaataccc gttgcgcctc tcgataccaa ctcccaaagc
    30721 ggcggcaatg gctctggcca cttcgggttt ttctgcaata taaagtcggg acatacctac
    30781 tcctctatcc atatcggttt tacgacatca cggatatgct ctctccggac tatgccgtaa
    30841 taacgcgcgt caaacgaatt ggccaccgca ttaccaatca aaatgtactc atcgtctttc
    30901 agacggcctt ccccttcaaa taccggtaag ggctgcccta gcccgtcttg ccataaaggc
    30961 cgggtgccgc caagcagccg tccgttaatc aacaaccctt catcggtaaa acggtaattg
    31021 tcgccgggaa taccggcaac aaacttcatc aacccggaat aattttccgg acaaaaaccc
    31081 gctctgatat accccctcct tcttgccatt cggaaaactt cggtatcggg agggcaaaat
    31141 gtaatgtagc ttccgcgccg taccgcttcg ccggatatgc ggtaaacccc tttggccaca
    31201 ctgcgggtat cgttgatacg gaaaccggct gcatataaca gcccgtagcc tgttaccagc
    31261 aatgcggcgg tcagacacat ggcaagcatg gcacggtcaa acagccttct catagagtgt
    31321 caaacgcgct gggcgtattg gcagtctttt tctgagtccg gtcggtatgg cgcggaggcg
    31381 ggactttgga gcgcgcctca aataccggat ctttgaaata cagggtctgc cgcccgtaaa
    31441 tcgccggata cccggcaaca taaatgacca tatcgcctgc ctgagtgatc aaaccccttg
    31501 cgtccttgac cggcccgggc atacgcatac attcgtcggc tgtcagcaac ggccgctgga
    31561 cttcctgcgt agtccgggaa acctgcccca acatcgcact tacccgtttg ccgctggttg
    31621 tgatctgctc tttgacaacc gttgtgatgc cggtcagctt ggacaggtgt tccgccgtct
    31681 cgatccggtt aggcggataa gcgttctgca catggcagct ggacgtaatg gtttcatccg
    31741 ggccataccc cgtttcccgg cttttcagtt ggttcaagtc ttggcagatc aaataacatt
    31801 tgatgccgta accggcgata aacgccaagg attcctgcat gatttccaac ttgcccaaac
    31861 tcgggaactc gtccagcatg gccagcatct tgtgtttgta gtggactttc ggcctcccgt
    31921 tttcaaaatc catcttgtcg gccgacagac ggataatcat attgatcaga atccgaacca
    31981 aaggccgcaa ccgcgcctta tcgttgggct gggtaatgat gtacagactg accggatctt
    32041 cgtcgttcat cagatccttg attttgaaat cggaacgact gacattggcg gcgaccacat
    32101 tgtcgcggta caaagcgagg aaagatttgg ctgtggaaag aaccgatccg gcctcctctt
    32161 ccggtctgtc cagcatatcc tgtccggcag aagaaatgac cggcagagga cggccgtctg
    32221 catggggata cgtcgccatt tccacccata aatcgcctac cggtttctcc ggatcagaca
    32281 gcatgcggtc aacgcttggt aaggtggcaa ccccgccatc atccctagct ttgtaaagcg
    32341 catgcagaat gacgccggtc agcaaagcga aagctgtttt gtcccagtgg ctgttcagcc
    32401 ccttgccgtc cggatcgaca atcattgtgg ccagattctg aacatcgcct atctcatact
    32461 ccgtccccac cctgatctca tccaaaggat tccatgccgc gctgccttgc gcggaagccg
    32521 gttcaaagcg gattaccttg ttgtgcgcgt atttcttccg ccacccggcc gtcaatgccc
    32581 ataattcgcc cttcaaatcg gtaatgaacg tactgtgcgg ccatgacagc aaagtcggca
    32641 ataccaaacc caaaccctta ccggatcggg ttggggcata agtcaggaca tgctccggcc
    32701 cggaatggcg cagataatgc agggtaccct ttttatcgac ccagccaccg acatatacac
    32761 cgtcgctgtc ctctcctttt ttggccagca gaccggccgc ctcgatgtct ttcctgtccg
    32821 cccaacgcgc cgacccgtgc agagactcat gcgccttatt ggtattcttc cgcacaatat
    32881 tccataccaa cagccctatc atgccgacgg ctaccaccat caacatgatg ctgaatgccg
    32941 tcataaactg ctgctcgtac cgtccgtgcc atttaccgta ccaaatcaga atgctccacg
    33001 gcatatagaa cttgccgatg ccgtatccca actgggaatg gtattggaac tgatgggcaa
    33061 aatattgtgt ggctgccccc ataccagcca acagcaccaa taccagacaa ataaagttta
    33121 ccaaacggcc gcttcctgtc gcttgggctt ggttctgatg gccgaccgca ttgttgcgtt
    33181 tgttcttgct gcgaaacatg tttatccttt gaaatttaac gatgccttcc cttaacaggt
    33241 ctgatctgcc ccgtcggagt aactttcact tcactgccca gtttcacgct cgaaaggcgt
    33301 ctggacacat agggtgtaac ggacatgacc agtatttcct cgacctcttt cggctggaat
    33361 aaagccagtg cctgcccgtc tatgttgcgg atgcccctga acaacatcac accggaatca
    33421 cttggattat acttcctatg attcaatata tcgaattttt tgtcaatttt ctcctgccgc
    33481 tcggcaatat atttgtcgat aggtgccaat gaatgagtaa gtctctcgtt ttcttgtgca
    33541 ggctcaggat ttttcggaat ctctactacc gcagcggatg aaattttgat ttcagccaac
    33601 ccttccatcc cccgcaaact gccccgcctg aatcccggcg gtttttttcc cggtttggca
    33661 accaaaccga caactgcctg catctgcctg atctcagcta ctcctcctgt ccgatgtatt
    33721 gtccgtcgca cagcctgata gtttgcggat ccttgctgct ccaagtgact agaaacatca
    33781 cgcggcaata gcacttgagt tcgtccttgg atgcgaacca gactgcgttc gggcattcct
    33841 cgcagactgt cgcttcctgc ggaagcagac tgccctcttc ctgctccaat gcctccagag
    33901 tcggagagct tcgggattgg gtatcctgat atgcctgcat ggaagcctcg atttcggctt
    33961 gcgtcatacc catgccatcg atagcgtcta ggctttcctc ctgcatatcc cgcgcctcct
    34021 cgttgctgcc ctgcccgccc atctgcgcgg ccagttccat cagcttgtct gtatcgatcg
    34081 gtacgttccc tgacatattc gtattccttc acatactgtt gataaagatg gttcagacgg
    34141 ccgtctgtaa acgacacatc caaacccttt gcggcggcaa ttctggccac cttatcctga
    34201 aaatgccgcg ttccgccgat agtcaggact tttccgtatc gccctattgc gatttccaac
    34261 gcccttgata ccgccttatc gtcaggaatc gcggccagtt tgagcacact gcccgtatca
    34321 cggatggtca tgccgtcctc ggcataaata accgtcccct ttttcgttac cgtatcaacc
    34381 ttgatcccgg caaatccgcc actttctttg ccgacaaagc ggttgccctt gtaacctgca
    34441 tccttccccc tccttctcaa cgcatctaac gcccggacat cgccttcggc agcacggtgt
    34501 ttcagccaat ctgcccaagc aaaacccttg ttctgctcaa aaaaaaccgc acgtcggcgg
    34561 gcataatcgg ttcttaccga tctgatttca tccttcattt gcctaaacag catccgatac
    34621 agaatgcgcc ttgcaatacc actgctgcta tgcctaatca ggctgcggcg aaaagcgtat
    34681 ttttctttga tgcggccgat ctccatatcc cggttttgtg ccaaatgcag gaaaaaatcc
    34741 tgccgcctgc ttttctgaag ctctttttcc tccttgtatt gccggaacag atcgtccacg
    34801 ttggctttgc tcaacggttt ctcacggtac gttttggatg gtttcgcctt tactcctgtt
    34861 ttctgatcaa atgcaccaag ccttttttcc aacgccgact tggaaaactc cctatctacc
    34921 gaacttgcct tgacatgatg ctcgccgtcg ctgataacaa aaccgttacc cctcagcttg
    34981 atttccaatc cgtgatcact cagtacatgg tacagttgct cccatgtctt agcctcttta
    35041 atctgctcac cgcactctct cctgatccat cctatcaggc tctcgatacc caccacttcc
    35101 tccatatcga tttttttatt gccgcgcccg ccacgctgca ccctctgcaa accgtactct
    35161 tcttcaaact tcctgcactc ccgccctaaa attttgaagt cgttatacgg ctcgtggatg
    35221 gtcttttttt ctgggtgcac tttgttaatc gctacatgaa aatgcagatt gtcggtatct
    35281 ctgtgaacaa cagagatccg ctgatgttcg gcatacccca aagcctcaat catgctctgc
    35341 tcaatacggc tcaactgttc gtccgtcaaa aattccccgc ccgcaaggga taccatcaga
    35401 tgataggttt tatccgccgc actgcgctta ttctgatcct gaactgccaa agtctcctga
    35461 accgcccacc tcaaatccga tgattcatag ttatgaatct ccacctttcc cactcggttt
    35521 gcgacacctt gagaaccggc cagataggta actaagcctt caaaactgct caggttggct
    35581 ttacgcatcg gaattttttt tgcaatcatg ttcagcctat ttttttagcc gctaaaattc
    35641 tgcggctccg cacaatctcc aacatacttt cctgcatttc tccaatttgc tccaacactc
    35701 cgaagatcgc cttagccaac tgatcgtgat cgaacatcat cagccgctcg tcgttggcca
    35761 accacatctt gatcaagccg cccaatcggc cttggtcggc attgattttg gccaaggctt
    35821 taaccgcttc accgtcataa acggatgcag gcttgtatcc caagccaagt aatttcaaat
    35881 aagccgattg gctcaatccg acagatttag ccaaactctc tatggttttc cattcttctt
    35941 ccgaaacgta aacgctgatg tattttggcc gttttcgtgc gcctgccatt cttggagtcc
    36001 ttaatttgtg ccgcaaggca cacaacagcc aacggtagtt ggccagcctc gcagagcaag
    36061 ataatccgtt gagcctaccg gcgaataagg attgtgatgt acatgtacat cacatatctt
    36121 gccccaattc tggggttcac cccaattctg gggttcaata cgccaaggat tatacgccaa
    36181 ataattcaat atatcgaaca tattttgatg acaaaccgcc taaaaatgct ttatcacaaa
    36241 caaaaaatga tttactatat cgaattattg tttttaaaac gattttgctt ggcagttttg
    36301 gcaaggcttt gtcaaaaaat tggcaaagcc ctatcgaaat gatccaaatg ggaaaaatag
    36361 aaaaactggt tttggccgaa cttgccgcca tgcagaaaaa aggctcctca gccggaaaca
    36421 aggcaaggtt cttggccatg caggaagaca ttaagatact gctggtggac aggaatatcc
    36481 ccgttatcag tgtttggcgg attttgtcgg aaaaaaaata tgtcccatat tcctatgaga
    36541 catttaaccg ctactgcaag atgtatatct tcaaatccga cactacggcc gctgaccaaa
    36601 cagacaccgc ccagtccaca caggaaaaac ctgattccat ccgcacggac aaagaaccgc
    36661 cgtctcccgc ctccgaaccc attatcgcgg gaggcaacac caaaagctct tcaggcttta
    36721 ctttcaacaa caacccagac ggaaaggact tattttaaat ggccattatc catatcagcc
    36781 tccaagcaaa agggggcgtc ggtaaaagtt tcttggcttt gatgctgtct atgttttacc
    36841 gccatatcga aagaccggta aaaattttcg ataccgatcc ggccaacgca accctgttcg
    36901 gttttcccgc actggaggcg gaaaaattcg agatcatgaa aaaggatgat attgacaaac
    36961 ggttgtttga ccggcttttc tcacaaatcc tgtctgcaac cgatgatgtc atcatcgatg
    37021 ttggcgccaa tgcgtttatt ccgctttgtt cgtatatcat ttccaacaat ctgattacca
    37081 tgctgacgca ggaaggccat caggtcataa tccacacttc cattgtcggc ggccagaacc
    37141 tgaagcatac ggtcaacgga tttgatgccc tatgctcgca atttccggaa tcaaccggtt
    37201 tcgtcatttg gcttaatccg ttttgggggg aaattgagtt tgaaggtaag tcgtttgaga
    37261 aattgaaagt ctatgagaaa cacaaatccc ggatacgttc catcattaag attccggaat
    37321 gggacgcttc tctgcacggc cgcgaccttg ccgccatgct ggaaaagcaa aaaacattcg
    37381 acgaagcctt agcgtccgaa gatttcagca tctttgaacg ccaacgtttg aacattatca
    37441 aaaacgaaat gttccaaacg atagaaaccg cccaaatcgt ctgagtacat catggatgaa
    37501 aaagtaaaag ccatattagc caccattgtt gaaaaaaacg gcatcgcctt atctcccaaa
    37561 gacccgataa tggttgtatt cactgcctta gaatatatgc tctccgaaca gcacgcagag
    37621 caaaaggctc taaccgtctt attcaaagct caggtagaag aggccacggg aaactggata
    37681 cagtccgttc accaacagac tgaggaactt gtcagccagc aactcaatac gaccaaagaa
    37741 caggcaaagg aaatgtccaa attactgctt catgagacag acaggctgct tgagcagaga
    37801 attactgatg ccgtaaaaga ctttgaagga aagctatacg gcatcaacga caacgccatg
    37861 aatgcagtag aattgagaat aaaggaaact cggaacacgg caattctcaa tctgacggcc
    37921 gccctgattg tcttgttagc agtcattatc ttggcgttca aatttacctt ctgacaaaaa
    37981 acccggcaat gccgggtttt ttgtctccat tctttacgcc aagagaaaaa tcagcgtact
    38041 attttttgac ctagggacag gcttaatcca gcttgccgtt tctgttgttc tttttctttg
    38101 cgctgttttt catgcagatt gtgaaccctg acagccgttt gaacatcatc aaattttttt
    38161 gccagatccg gttcctccat ccttaacttc ctagcagcca actcctgtat tttcggaaca
    38221 cccagagcca tcccgtcgcg aatctcgcga acatcttcca acctgtcatg cagacggttc
    38281 agccttgatt gtgccactgc aatgtttgca ctccacgtct gttttttgga cggcatgctc
    38341 aacattcccg gccgggcgtt ttgcaatgcc tgcaacttgg ccatttgccc ggaaatcaat
    38401 tgctccaatt tttcttctat gcgctcaacc tgttctgtct tttccaagac ataagtattt
    38461 aacacaccgg cgaaagtttc ctcaataacc tgcctgcttt caaacaactc ttcttgttcg
    38521 gtttcagccg caacagcttt agctacaagc cctttatctt cattcatggt gttatcctaa
    38581 aattttagcg gctaaaattt catttagcgc gttaaagttt ttttctctcc gatacccgcc
    38641 ttttctactg ttgccgcttt gtccttgaca ctgattgaga ccacttctcc gatcgacgga
    38701 acggaagaaa atagagaaca cgggtatttg actatagact gtcgtgtttt ctgataaaca
    38761 tagctgtcat tttgaaaaac gatttgccct tcgtacctga cagaatcctg aggagatttg
    38821 gccgtatgca gcaaataaat tcccggcttc atattggcag gagcaggctt aaccgcctgc
    38881 gtaatccatt tgccgtcttt ttctgtctcg aacaaatgct gtttgttcaa taccagcact
    38941 ctttgttttt ctgcttccat tctgctaaaa attttcaaaa aagatgtgaa taattctaac
    39001 agaacagtct ttaat
//
LOCUS       DAXDSD010000004        37378 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXDSD010000004 DAXDSD010000000
VERSION     DAXDSD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37378
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.004"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..223
                     /locus_tag="ACFB2Q_000132"
     CDS             <1..223
                     /locus_tag="ACFB2Q_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917256.1"
                     /translation="TLNLFVEGFWIIGLLQAAVFTFVVDCSSSDLGSLSQWQIGNDWD
                     RQMDPFYAHEIGNIWHGTHLDSSSEEYWK"
     gene            complement(280..438)
                     /locus_tag="ACFB2Q_000133"
     CDS             complement(280..438)
                     /locus_tag="ACFB2Q_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917257.1"
                     /translation="MSIAQIVVQIEEVDEGNYQVRMSYSGGNDLVSEEGKSAFEQIIT
                     SIGKVLET"
     gene            complement(442..1023)
                     /locus_tag="ACFB2Q_000134"
     CDS             complement(442..1023)
                     /locus_tag="ACFB2Q_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFC0917258.1"
                     /translation="MRNATSYGGNGVMWRGISSGRHVRGNYVLKASTAHNQAELERYV
                     HNPQVRAFLDFIAASEGVKHGYQTAIGNYRITDFSRHPGNSVRRSFTRTDGSRSTSDA
                     SGRYQFMGYTWKGLAKQYGFTDFSPATQDKAAVALILAKKGAMEAILNGDYEQAVMKL
                     GGIWASFPTAPNEYRQHKRSWGFVHNFFRQRGF"
     gene            complement(1029..4031)
                     /locus_tag="ACFB2Q_000135"
     CDS             complement(1029..4031)
                     /locus_tag="ACFB2Q_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFC0917259.1"
                     /translation="MMAVEYFTFPGNGEAVRAVFNGVATISGSTMMGGAMQAAALFGF
                     LVTLAVAVFKLDLKDNFSYLFVVLFCWMGMMVPKTTVLITESGGYGYTGRQYTVGNVP
                     VGLAYMGYFVSSFGQSITRKAEQVNHLPDDLNYSRTGMLFGTRLMENIREARIPDAQL
                     TQDWALFMYQCSFFDMNLYHFYNVQDLAQSADILATLTHTNQALFTNVSQIASRRGNM
                     LQYNGKSKTMTCNEAAKELKDRTRFYSKNYLPGYIADRVFAGLGTNSAGINRVNALVT
                     LGNSSFQYLLDNARFDTLKNIEQAAMVEVIRQAGIINGQRNRNPAAVQQAFAQVQARN
                     QYIAAQKTGSSMASWNLPLIRSAAEAILIGLFPFVMILAMLGGIMAFRLLSFYMMSML
                     WIQLWAPVASIINMIMTMNAKRLFSVEAANGVITPGTGDTLLMAAADAQAAAGAAMWL
                     IPVIAGALAMGGRSLMNGMMGMTSSAKSTGEAAGSQVGSGNYSAGNMNYNNSNANKHS
                     LNPVYTDPQMMTAQSAAGTSWRNMATGDSRAQMRNSSFGVSSQSQISQSESYSKAADR
                     AETSAQQWQASFRESVSQGNANRLAFATNYGMDRSASEGYGMGLDAAESRKLSYVIDQ
                     GKKIAQKFGVTNTSAVISAIAAGLGISGNTGSSTKAQNIKNALGAKGDLGWSGKSQNT
                     DQMVAEIGESIEAGKRQGISFDSNFTDKVSQSEAYKVALNSGNSLAQTAEANFSKARE
                     ASHMASESYQKAQSYREMAAQTYGNGITIAQDNTNALVQDNPELTMSELNAGQGGTYN
                     AAIRAGVNSQDRLKAAAENSGWKPNDLSPEDRPQNNTQAAYKHFSDKVSSGAGAAFGD
                     VQTQAHQAGIIPDGISAAINARGENLKQEYANKSDKVEGDINQGRNSHRQEVGRLATH
                     AKNAQDQTNGVTMRINTHALPWNSFGERYDNAMKDVREGKGYIGSGSDQAAAHLGNPA
                     LASEFDLPIPQRPGNSKSSGSAVFRPKE"
     gene            complement(4038..5549)
                     /locus_tag="ACFB2Q_000136"
     CDS             complement(4038..5549)
                     /locus_tag="ACFB2Q_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFC0917260.1"
                     /translation="MKFHIFRFPKPAKVPTVCASSAKYLCLAASLALPINSSAGIEKN
                     MADMFNSMGAEANYTEAGAFHGQSGSLYTGGGMSVRFPVSDISLGNIQLPSISAGCGG
                     IDFFAGSFSFVNKEQFIQFTRNLGNNAAGVAFDLALKALDPMIQDAIGGIRDLVNYVN
                     QNSLNSCKLSQDIVGGAMGKLGSLASSNCRAASVDSGSSNDPAEGAWYCQAAKNLVHE
                     ADKVRNTGKPQDTISFTGGNLTYEAMKNYTGQRSKLGIDMDFYYSMLGTIVFTPLTQH
                     EGDKDKYTVGIQSFEPKILSIQDLLNGKNNAGGEIREKVVLDMWTCKDGSKKLMENCT
                     EKNDVEIPSLRYMVHKRIETLPTAIRNNQGWNLSTRKQIAAIVNNSKLPILKMAVSDA
                     FLGTGYLKKTAVLDAIAVDIMANILDQSERQVRSTLGMYNKTDDASGEAVRKLFANLG
                     ELRSRIYQERIDSMKKIEIEESMMRAMEQFETQWRSAFVDTNNSMTFDSMNRF"
     gene            complement(5721..6497)
                     /locus_tag="ACFB2Q_000137"
     CDS             complement(5721..6497)
                     /locus_tag="ACFB2Q_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFC0917261.1"
                     /translation="MAVMLIFSLHASNLRAADDGMGWYDQDIWKNENRAFLWYPDAKK
                     PKQAGQNRPQNTPVRNELAEFEMLQRNLDESRKIAVMNPTPQNLKRYIELQEVVMNKS
                     AAFADQWQRVIWQNPNLDYSQRGRPNNQLAQQQYDHNRQQQKAGAIRKLAGENGIMFV
                     FKSDCPYCHTMAPILKNFADQYGIKVMPVSLDGGGINHFPRALPNNGIAQRLNITTVP
                     AMYVMDTKTKQFTPIGFGVMAQTTLEDRFLAYSRPVGTLY"
     gene            complement(6509..6934)
                     /locus_tag="ACFB2Q_000138"
     CDS             complement(6509..6934)
                     /locus_tag="ACFB2Q_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917262.1"
                     /translation="MNINNWLAASAGIVSGMFFTGMALAASLPYTGGIALEKTEALAR
                     TSIPLPDGKPDALLALAMRHGSAKGYFSGRAAAAVKQKFRQDIPIFVEAVRMEEIKNK
                     PGCNRVRLTFHTSPALSQTFPSEIMDIGACPNRKNGGKK"
     gene            complement(6921..8462)
                     /gene="traN"
                     /locus_tag="ACFB2Q_000139"
     CDS             complement(6921..8462)
                     /gene="traN"
                     /locus_tag="ACFB2Q_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFC0917263.1"
                     /translation="MLITSASAAALRESAACTRTSSVCVDGPSTKNINGVDVTKDCWE
                     YKEEYQCLEKDSADYCAPLKDPSAKCEVQGQTCLEQSNEGECLRYTHKYSCDVDLRTL
                     HQGRLPTKVEEMEHTHLISSQWDESSCQVQGKKCKAVATECLEPGSTKTINGVPVTRD
                     CWKERRTVQCTDGSDSETCSAYTSSDQCRLIGDKCTHQLPDGTCQAREKQFECTEKGE
                     TTKEVSGCQDRDFAKTMTTMEFARETQRFYDPEKQRFFNGEAGQCSIKLDGALDSVFG
                     GDCCRTKADPGKFVDFAVQTGTTMATTYFMASVASHYTFTTMFVSSAAQAMGTALSAA
                     GGITGTSQIGALGFSAAGQQGMGVIVGFNPAVFAAAIAVIAIQQWLKCPQSEILVAMK
                     RKADLCHYVGSYCGSKILGACVTMIESQCCFISKLAKIVNVGGKEQLKRGWGTPENPK
                     CEGFTAQELEQLDFSKLDLSGFYEEIYANMDNVAKQGQKVSQKIREASVNGKNLEVKN
                     YYEYQ"
     gene            complement(8501..9745)
                     /locus_tag="ACFB2Q_000140"
     CDS             complement(8501..9745)
                     /locus_tag="ACFB2Q_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917264.1"
                     /translation="MKRHAIYLTVFGLMQTAFAADYGSYVGELGKLGAGTATNMMQPA
                     QYTPRYTDNVPMAEQYYGGGVSLPTQFGDAKFNDCKNLPANPDLYLRQECEGVNLIAG
                     SRTKRPDVTISSNEKLVKGTQKIAGDPAETLEKYKWKYPVNEDGSVGTVPSSACPTET
                     ITVPAVKTVKRCNEYHGAEMFLCEAALKVNVDPGWNYSCLESKYRNQQYQCGKKLKVV
                     CEKSGPNCTRSGIEAGSLQGDMTVRLTASGSAHRLMFGTIGDNYWRDGQYDREMTLNV
                     KNPEKLTLFTLSRVEYDDWLIVKVNGKIVYSSYNNQMFAADYTAKDEDGFPIGRRFAP
                     VRNEEGTRLGNAERGKSWRKNLNIDIRPYLHQGKNTIWTRTVVGGGGENAIFFNVHQY
                     CEPVCHDKWENSCSEYEKRVKQ"
     gene            complement(9742..10494)
                     /gene="trbC"
                     /locus_tag="ACFB2Q_000141"
     CDS             complement(9742..10494)
                     /gene="trbC"
                     /locus_tag="ACFB2Q_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFC0917265.1"
                     /translation="MDKSLRNVIVAGGVFLILLGGLAGVGSAYADADVEAARNRLRTT
                     MGKVTRVKNPNVGDNRQQPEIPDLKGIKHRVPDVRDYGNAKIKADPMSIAQKYRTASQ
                     EARQAAEKADLMVFVSFSMPEASLKRIARETAKAGGVMVIRGFKDDSLKATVKAAEEL
                     AALQGDMLIHPELFDHYGITEVPVTVIARNSEDGLDGCAVNSELGMCTEHVAVKGDVS
                     LHSALEHLIRESEDKKLSDIANAKLAVLEGRK"
     gene            complement(10509..11627)
                     /locus_tag="ACFB2Q_000142"
     CDS             complement(10509..11627)
                     /locus_tag="ACFB2Q_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFC0917266.1"
                     /translation="MKIFVTPLKLLIPLLLLPLFSISHAAEAVPTAPIPNKLTCQGKM
                     LNPINDICWSCMFPIKIGGKINLNMDNQEDNGSSPNNWFCSCTHPSRSGVTVSFWEPT
                     HLVEVVRTPFCMVSLGGISLKNQKADTAETTGKADVSNKIQNLFGGSHKRGRNEQHDQ
                     HNAFYQVHWYKNPVVFWLEALLDNDCMEKGTLDIGWMTELDPTWKYPSLAVYQAPDSA
                     LFANVVAQAACAADCVKAAAGFPFNKLYWCAGCHGTIYPLSGWMSGHFSEVQAAKLMA
                     ARIQTKMHRDLVAWRTSGRDALCGYKLNPVMDKQNYKMAMTYPVAARKFRGRCCEPIG
                     RSTLVRDSGKAFPYKGNQFAFQLFRKRDCCGAYKFKTN"
     gene            complement(11624..12280)
                     /locus_tag="ACFB2Q_000143"
     CDS             complement(11624..12280)
                     /locus_tag="ACFB2Q_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFC0917267.1"
                     /translation="MSHKKKIASVASLVILATGISSWVAASTPSVEKVGDKIGTTYPI
                     AEKHAIREIEARLKAKQDSGQLAAMQKEVQDRINRSALNLQPLEGLAKATKGSVRYFD
                     PSYTLTETIYDQDGQIIAPAGTVVKPLEAAPIPFKMFFFDGREPDQIELAKKLAAEYG
                     ESFMPILTAGNWYELSVEMDQAVYYDQEGRMSKSFQLNEVPSLVSQEGGRLRVEVMKP
                     "
     gene            complement(12289..12834)
                     /locus_tag="ACFB2Q_000144"
     CDS             complement(12289..12834)
                     /locus_tag="ACFB2Q_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HFC0917268.1"
                     /translation="MFEAMIKISQKESNKAVMKRLYREFCQKYATPFLAVAVCLSAFN
                     QHYALAFNLSRSLPHHLYFIKKDANKLSDLKQGDYVAFAWQGGFYPIGTQVVKEVAGL
                     PGNHVTKANRTFFIDGKEVGTAKEYSLDGMKLAINPFEGEIPQRFMWVKTGHKDSLDS
                     RYELSGLIHSGQIIGKAVPIF"
     gene            complement(12834..13382)
                     /locus_tag="ACFB2Q_000145"
     CDS             complement(12834..13382)
                     /locus_tag="ACFB2Q_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917269.1"
                     /translation="MTEAKEKKENTANTDPEKNPEDNGVEVSLGSLVIGAISIAALAL
                     GVWNFMKTHEKPVHIVDLAGISMTYREQAKNQGLQNGISDRQRAAILQNYQAKMNTLQ
                     QVIEEKVIRCGCNIFVKSALVGRNTEVVDVTADIMKELDKRVPATAVVAAPVHQQVEK
                     TIPKPMAEEGKGTPVTEEEMDR"
     gene            complement(13410..15986)
                     /gene="traC"
                     /locus_tag="ACFB2Q_000146"
     CDS             complement(13410..15986)
                     /gene="traC"
                     /locus_tag="ACFB2Q_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFC0917270.1"
                     /translation="MGVLSKFKNWLAGSDDVDQPMEALHNNPVDPDFDHLSDYLNYGA
                     YDTKTELFTLMSVKGDKPLGMGFILELNPFLGGSDDLIGKWGSLYALLPEKTPIQVQI
                     FGSPDLRNYFKEYEQVQASRPIDDPMRSTFETLAKKRTEFWNKGTQKVLVENTAIRLR
                     NLRCILSVNLNIPPDNAVEVSKAVSLLARMRDSLRSMQIYGRTWNADDLLAWTTLLLN
                     PNRMFTGQQDEIDPVWDETKFLSEQMIETRTSIKVLDSGSGLRFGNRAAGDCVIAQCY
                     SANRYPEEFHLSNMGALIGDVIEANMNYTSPFLISMAMFKRDYDTSSNTVKLKAARAK
                     QTAESKMAAVMPEAAKIKRDYDICLEAFGKGGGGLVSLLHQVVIWERPENINLAESQA
                     VSIWQAQGFGLYRDQYLQLGSYLTALPMAIDKEVEKYLDSKKRWSTKTMTNAVCMSPV
                     IGEWHGLGRPVIGLFGKRGQAMGIDLFANPAGNYNFAIIGASGSGKSFFANEIVRNYM
                     GLGTQVWIIDVGRSYEKFCQMIGGQYIEFTYEAGLCFPPFQMVTDINEDIEMLKLIFS
                     LMASPSDSLTEQQEAKLQRIIEEVWYEFGQEGTVDHVRERCLKCLITGSNEGGKPGER
                     DLSMVAVADQLYTYCSEGIYGSYFNGKLNVEFKNDLVVLELEELKTKPDLQQIIMQLI
                     MYRIMQGMYLSRSQYKIMMIDEAWALLGDTGTAARFIEEGYRRVRKYKGACGTLTQGA
                     NDYYKTKAAEAALENADFVFQLRTGAKSLKTIQDKEPFEVNDGIMQILRTLSKTDYYS
                     EIFVSTPIGYGVGRLYSDPFNVLASSSKAEDVEAVNHYRNQGLNTAEAIEAVLRDRAA
                     TR"
     gene            complement(15996..16577)
                     /locus_tag="ACFB2Q_000147"
     CDS             complement(15996..16577)
                     /locus_tag="ACFB2Q_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HFC0917271.1"
                     /translation="MMRLKALSITAAILILSGCSTLTMSGIGGSEKFRCPNARNSDDP
                     YCESISSNYKASVAGVLKDGMKRQVGQPYTDQSVRTLMLTQAYSSGTPVRSQSEIARI
                     WVAPYLDTDGDLNDQSFTYVVLNQGDWLIAHNGQQIINEYRPIRLLGDGAKTSGSSVE
                     TSPTNSNNTNDRIPDVGVNLHLQETSGIPGTVR"
     gene            complement(16574..17293)
                     /locus_tag="ACFB2Q_000148"
     CDS             complement(16574..17293)
                     /locus_tag="ACFB2Q_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC0917272.1"
                     /translation="MKKKILATIAAITAFQTAFADVKTVGSTLHKLYPNTTFSSVKAT
                     PMASIYEVTMGDNIAYVQENGRYFIFGALYDMQEQKDLTEMARSAVTQKSYSRLPFKN
                     AIKIVKGNGGKGKREFALFSDPDCPFCRRLEETLAGMTDYTAYVFMFPIKSLHPDAIS
                     KAEHIWCSKDREKAWNNYMLMDKEPAAGNCKNPVSENIALAEQLKVRGTPSMIHKDGR
                     RTSGAMPRAELEKWLNGAGAE"
     gene            complement(17303..18616)
                     /locus_tag="ACFB2Q_000149"
     CDS             complement(17303..18616)
                     /locus_tag="ACFB2Q_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFC0917273.1"
                     /translation="MRVKVNKFKLSAARKRQLAIAGMVAGSMAAIVGSAIYVIEGNKK
                     KSAEVTQVQNKRQILRTDFTSPQAGITDNSLWMNTESSKIEYANRKISELETMVQELK
                     EKENSSNPDTSKGLGPDGLGKPPAISGIGDNGRLPPAPPAGTLPNGAPPADRPIERKI
                     VSGSMSEAELQPGTTGSVNTGNPNENVRVSPQLKEAEWVKSKTPRMNIEVVEDGGKVV
                     VSQGKFRARDSYIPSGTFFRSVLLGGVDAPTGGEAQNASPHPVLMRVTDFAQLPNRFK
                     YNFRECFVTGQAYGDISSERAYIRLQNLSCVGTDGRAIDMPVKGYVAGEDGKTGVRGN
                     LVTKQGQLLANALMSGVISGMGKGVSEAFKVTNNTAFGSTTSIRGSDQYRAGIASGIG
                     GAADRLAEYYIKLADKVFPVVEVNAGRQVDVVLTQGIEIDTGETK"
     gene            complement(18618..19352)
                     /locus_tag="ACFB2Q_000150"
     CDS             complement(18618..19352)
                     /locus_tag="ACFB2Q_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFC0917274.1"
                     /translation="MCSLLKKIVFSALLAAGPATSFAAQRVPATENTPHVVSISKRNL
                     SRIAIEGGRISSWKFMEGDLELQKDTTTGQLFVRSLTSNPTNLFVISEEGKTYLLVLK
                     PTSKQGDNIVIDVAGANRREAAVLASQTGPMPVTMNSTEYVRAIKKMMASMMRGTTGD
                     MGINHSHEYQTVPLWKNTLFIQNGSYTAADMQGLSFTLTNLGTKQLEIREQEFYRQGV
                     LAVAVRKQILQPGEITDVFIISRPGG"
     gene            complement(19354..20019)
                     /locus_tag="ACFB2Q_000151"
     CDS             complement(19354..20019)
                     /locus_tag="ACFB2Q_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFC0917275.1"
                     /translation="MKTEDMLSELAIRTGIKVFFWILLGMSIAVNLFLSATLLFKGTI
                     VQTTLTPPEIRRSMTVSNIAFSKEYLEEMAPYAAYLLLNVTPKTVDYQNSQLLKIVSP
                     DYKDALERELSLNALWVKKNNVGTTFSATEATADISDNTVSIRGVFEVKRNNALVEQK
                     DRELLISFKNNYGTLQLLSIKEVQKRVTKAEPAGQEKSEVETEDVEISKTATEFKGGN
                     RGN"
     gene            complement(20016..20297)
                     /gene="traL"
                     /locus_tag="ACFB2Q_000152"
     CDS             complement(20016..20297)
                     /gene="traL"
                     /locus_tag="ACFB2Q_000152"
                     /inference="COORDINATES: protein motif:HMM:TIGR02762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFC0917276.1"
                     /translation="MRDYHVPVSIDDPKKAFIWDLDVFILFMSGFGIGIVSRHFFISM
                     SISLFIAWRWGKLKSGKHQWFFLHVLYWYLPINEKNKRIPETNKREFLN"
     gene            complement(20319..20615)
                     /gene="traA"
                     /locus_tag="ACFB2Q_000153"
     CDS             complement(20319..20615)
                     /gene="traA"
                     /locus_tag="ACFB2Q_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraA family conjugative transfer protein"
                     /protein_id="HFC0917277.1"
                     /translation="MNIKRNKLVKSASAVALALYANLAMAATTGAEFKGLADMVTGWS
                     EGYLGMALGLTAFLIGLAVGLMKQTIMPCIVGLGVGLAATLGPGIIKGMFTLII"
     gene            complement(20671..21192)
                     /locus_tag="ACFB2Q_000154"
     CDS             complement(20671..21192)
                     /locus_tag="ACFB2Q_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC0917278.1"
                     /translation="METIKNTLFVASAMILTACAAPKQEVAGLPQDATYTVEQNQLGE
                     FVFCRNEECSGFERKVLAEQPVYAQTQTNLQKDEIRVFFNLGSSHLGAEGVRTLKRVL
                     PKLKNTQTVYLRGWADSIGGRNTAINRRLAKQRAAVIGKWLRKHNVSAKIRTASSPAC
                     CNSDDTRTVVITW"
     gene            complement(21162..21623)
                     /locus_tag="ACFB2Q_000155"
     CDS             complement(21162..21623)
                     /locus_tag="ACFB2Q_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC0917279.1"
                     /translation="MRLRAILLQFVVPGVLVLTSARAVAFCYKEAGSKYGIDPAMLQS
                     IALTESAFRPNIESHTADIGLMGINRSWLPVLHKKFGLTGADVWNPCTNVHIGAWILA
                     NSYRQHGKSWEAVGAYNAACTKLKGRACYRARMTYANKVYQNWKRLKTRSS"
     gene            22251..22553
                     /locus_tag="ACFB2Q_000156"
     CDS             22251..22553
                     /locus_tag="ACFB2Q_000156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917280.1"
                     /translation="MMTLDDDENTATFLIEKLEKLLEPLPIDIDNTTFAEALADDFEI
                     LFLMAQYGSEYWNSLEESFEEFCIRHTTLPNQLIADIPHAKKEKVTMWLKSLLKSS"
     gene            22550..25102
                     /gene="mobH"
                     /locus_tag="ACFB2Q_000157"
     CDS             22550..25102
                     /gene="mobH"
                     /locus_tag="ACFB2Q_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HFC0917281.1"
                     /translation="MKTSLLTIASSLMLVSGSMLYLINTRKSTDSVSHNDEVAHINQN
                     IVNDRFRILNAHELIQVLDLSPQISGIKMNLGLSDENWSKDALPFLEKYIAFVQRLPA
                     SESHHHAGDGGLVRHTLDVAALALVASTSQSWPPNAKTEEIAKKTAVWRYGIMCAALL
                     HDVGKTVTGFQVELFDSAISLEKLLWLPDTGSMAESGKLYYRVEFPDAKSAYSTHAEI
                     AWTFFQALVPSHVRQWLATTDPNLMITLRNYLSGKKDGSPLEQLIKNADMTSVSRDLR
                     SGSRQRFSTAKRKPFIETIMETLKEMLSDRGVHFSIATTAGGDLFRKGDRIYLMSKNV
                     PDYIRQYLRKNQHPAAGSFPADNQRIFDTLFEYRAVIPPENDPHRAINHIEVEFTRMD
                     GEKVRNIFSVLCFNAKTLYPDGDYPTEFLGNLSEVSAKKEIPDVVVSEDKVSTQIQGA
                     EDNPVTMTKDKMDFLSIQPVTAEQPPLPEKNQTETANSAFDNSMNTAAPAENKSSESE
                     KAKEPGYGTIDNLIENFNLIEADSEQTESTKSEAETAENAVNLVQENIRVEVKTETVD
                     KPRAEVKETETDKAPKTIKSGTKASRKKLAGLFADNRKPIGKTAEPDAQIKTTETVTH
                     QIDSEAVSLPESESAQNGMRELEQLRLREPTSSPRPVQVMDDSEGLDSLASGVKNMAE
                     LEAIVSERNLKKQSETESTEIVEDSVKAKHMEARDRGMQFLRWLADGLGDGSIAVNRS
                     GATVHFIEQGMLLVTPAVFRDYAGGVFNKSDTESLGPLTQKGFEALRLHARTKKSSLH
                     RVLTTNGSNRRLFYCYLIPEHNIKHIIQPGSRPQNNTDIKLDESDLLVMEQK"
     gene            25099..27141
                     /gene="traD"
                     /locus_tag="ACFB2Q_000158"
     CDS             25099..27141
                     /gene="traD"
                     /locus_tag="ACFB2Q_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFC0917282.1"
                     /translation="MSAHFPENVYRSPYEWISVAAYSTTAVSVAHLPVPPTIAYGSLA
                     IFSLVSVKRALSALRIMRFRKNIRKLPYYALTADEIPSSNKALFLGKGFRWTQTHTQR
                     LFMVRQPENDGLRNPSKLYKAARKHEIETMGTDWISRITRKSAWWNPVAPLPPVGGSP
                     ELHGIEIEEDDIWEDLGERVGHKVVLGTTRVGKTRLCETMIVQDIRRGDVTIAFDPKG
                     DADLMTRMYVESQKSNRPFYMFHLGFPEYSCRYNPIGDYGRITEVATRIANQLPSEGQ
                     SAAFRDFVWRFVNVLTKTMEGLSIKPSYENIYKSAANVDELAGDYFKKILDKHHPGWE
                     KDFDNFDMPESEAKSAVKTGRNLDTMKLGSFLKAKKHNEPLIDALGGILSNDRSYFEK
                     LVSSLYPLLEKLTTGEVAKLISPDTEDLSDPRIIMDWRKVMESNAVVYIGLDSLSDAE
                     VAAAVGNAMFADLTSLAGQIYKFGHGVGQSGKTQKRKVSIHADEFNELIGDEFIPLLN
                     KAGGAGYQVTVYTQTWKDVEAKIGSPAKAGQIGGNLNTMIMLRVKTVETAEMLTNQLP
                     EVKIMTSTLVSRAGDVAFPDSHEDFSSGNEDRIGAETVPMLTPADLTQLPKGQAFALI
                     EGGQLYKIRLPLPKKDKQEEIPAHIGEMAADMREVYRLSRPSAEENAFTEGSSYAV"
     gene            27131..27826
                     /locus_tag="ACFB2Q_000159"
     CDS             27131..27826
                     /locus_tag="ACFB2Q_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFC0917283.1"
                     /translation="MPSNTSAWYMNSLPLKFLFLPFKAAYTLFLIVLLIFGSSVLTQN
                     YFGTGNLLEDEIKWSESVGHDAFPGKEVDDTINLQIARLSYKGLKTVFFDLTLVNESL
                     KAKSEADFGYKFKNYLVPRMKYLQQFDTTLKVISLRLGNLALIVGISVIFAIPVLIDG
                     LVMRAIRQENASRESAGIYHRAKYWRTGIIWLGCMIYMCVPISIPAYLLYLPLVAAIW
                     MIFMQAKYLKKYL"
     gene            complement(28271..28783)
                     /locus_tag="ACFB2Q_000160"
     CDS             complement(28271..28783)
                     /locus_tag="ACFB2Q_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917284.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYILPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(28874..29269)
                     /locus_tag="ACFB2Q_000161"
     CDS             complement(28874..29269)
                     /locus_tag="ACFB2Q_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC0917285.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGLL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            complement(29256..31178)
                     /locus_tag="ACFB2Q_000162"
     CDS             complement(29256..31178)
                     /locus_tag="ACFB2Q_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC0917286.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFDFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATAPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(31255..31626)
                     /locus_tag="ACFB2Q_000163"
     CDS             complement(31255..31626)
                     /locus_tag="ACFB2Q_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC0917287.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            31822..33129
                     /locus_tag="ACFB2Q_000164"
     CDS             31822..33129
                     /locus_tag="ACFB2Q_000164"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC0917288.1"
                     /translation="MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNANMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            33122..33556
                     /locus_tag="ACFB2Q_000165"
     CDS             33122..33556
                     /locus_tag="ACFB2Q_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917289.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(33732..34001)
                     /locus_tag="ACFB2Q_000166"
     CDS             complement(33732..34001)
                     /locus_tag="ACFB2Q_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC0917290.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(34183..36645)
                     /gene="lon"
                     /locus_tag="ACFB2Q_000167"
     CDS             complement(34183..36645)
                     /gene="lon"
                     /locus_tag="ACFB2Q_000167"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC0917291.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASALLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            36897..>37378
                     /locus_tag="ACFB2Q_000168"
     CDS             36897..>37378
                     /locus_tag="ACFB2Q_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC0917292.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWYYVRESGADGILKATYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGVHEWHKKDTTLIDNS
                     "
BASE COUNT         9480 a         8953 c         8408 g        10537 t
ORIGIN      
        1 gacattaaat ctttttgttg agggtttttg gataattggc cttctccaag cagcagtatt
       61 tacgtttgtg gtagactgct caagtagtga tttaggttca ttaagtcaat ggcaaattgg
      121 aaatgactgg gacagacaaa tggatccatt ttacgctcat gaaattggta acatttggca
      181 tggaacgcac ttagactcga gttctgagga gtattggaaa taaaaaacag gagatttata
      241 aactcctgtt tttttatttc caaaagatgg ttaaattatc tatgtttcta gtaccttacc
      301 aattgaagtg atgatttgtt cgaaagctga tttgccttct tcggatacta gatcgttacc
      361 tccactataa ctcattctaa cttgataatt cccttcatca acctcttcaa tctgaacaac
      421 aatttgtgca atactcatct tttaaaatcc tctctgccta aagaaattat gtacaaaacc
      481 ccaacttcgt ttgtgttgcc ggtattcatt aggagctgtt gggaagctgg cccagatacc
      541 acctaatttc atgactgcct gctcgtaatc gccatttaaa atagcttcca ttgcaccttt
      601 tttagctaaa attaaggcga cagcagcttt gtcttgtgtg gcgggactaa aatccgtgaa
      661 gccatattgt tttgccagtc ctttccatgt gtatcccatg aattgatagc ggccggaggc
      721 atcagatgtg cttctactac catcggttcg agtaaaacta cgtctaacag agttacccgg
      781 gtgacgggag aagtccgtaa ttctataatt acctattgcc gtctgataac catgttttac
      841 accttctgat gccgcaataa aatctaaaaa ggcgcgcact tgcggattat gaacatatcg
      901 ctccaattcg gcttgattat gagcagttga agcttttaat acatagtttc ctctcacatg
      961 tcttccactt gatattccac gccacataac cccgttgcct ccatatgagg tggcattacg
     1021 caacggggtt attcctttgg gcggaataca gcggatccgc tagacttcga attccctgga
     1081 cgttgaggaa ttggtaggtc aaattcggat gctaaagccg ggttgcccaa atgggctgct
     1141 gcttgatccg acccagaacc tatgtatcct tttccttcac gaacgtcttt cattgcatta
     1201 tcgtatcttt caccgaaaga gttccagggt aaggcgtgag tattaattct catggttacc
     1261 ccattcgttt gatcttgagc atttttagca tgcgttgcca atctaccaac ttcttggcgg
     1321 tggctatttc taccttgatt aatatcgcct tcaaccttat cagatttatt tgcgtattcc
     1381 tgtttcagat tctcacccct agcgttgata gcagcactaa ttccatccgg aattataccg
     1441 gcctgatgag cttgggtttg aacatctcca aatgccgccc cagctccgga tgaaacttta
     1501 tcagagaagt gtttataagc ggcttgcgta ttgttttgag gtctatcttc aggggaaagg
     1561 tcattaggtt tccagcctga attttccgca gcagctttta aacgatcttg actatttaca
     1621 cctgcacgaa ttgcagcatt gtaggttcct ccctgtcctg cgtttaattc actcatagtc
     1681 aattcggggt tgtcttgtac caatgcattt gtgttatctt gggcaatggt aatgccatta
     1741 ccataagttt gagctgccat ttctcgataa gattgagctt tttggtagct ttctgatgcc
     1801 atatgcgagg cttctcttgc tttgctaaag ttagcttcgg cagtttgtgc caaactatta
     1861 ccagaattga gtgcaacttt atatgcttct gattgggaaa ctttgtctgt aaaattacta
     1921 tcaaagctaa taccttggcg tttacctgct tcaatacttt ccccaatttc tgctaccatt
     1981 tggtccgtat tttgtgattt accactccaa cctaaatcac cttttgctcc taaagcattt
     2041 ttaatatttt gggctttagt actactgccc gtattgccac taatgcctaa cccagctgcg
     2101 atggcagata taactgctga tgtattagta actccaaatt tttgggcaat cttcttgcct
     2161 tgatctataa cgtaagataa ttttctactt tcagctgcat ccaaacccat tccgtaacct
     2221 tcagaggcac ttctatccat accataattg gttgcaaagg caaggcggtt tgcattgcct
     2281 tgagatacgg actctcgaaa ggaagcttgc cattgttggg cagatgtttc tgctcggtct
     2341 gcagccttcg aatatgactc tgattggcta atctgtgatt gagaggaaac accaaaacta
     2401 ctattcctca tctgcgctct actatctcca gttgccatat tccgccatga tgttccggct
     2461 gcagattgag cagtcatcat ttgaggatcg gtatatacag ggtttaagct atgtttgttg
     2521 gcattgctat tgttgtagtt catattgccg gcagagtaat tccccgaccc tacttgggaa
     2581 cccgctgctt caccggttga tttggccgat gaagtcatgc ccatcatgcc gttcatcagg
     2641 ctgcgtccgc ccatggcaag agcacccgcg atgaccggaa tcagccacat tgcagcccca
     2701 gcagctgctt gtgcatcggc tgctgccatc aataacgtat cacctgttcc cggtgtaata
     2761 acgccgttgg cggcttccac gctaaacaga cgcttcgcat tcatagtcat aatcatattg
     2821 atgatactgg ctacgggtgc ccaaagctga atccatagca tactcatcat gtagaagctt
     2881 aataaccgga atgccatgat gccacccaac atggcgagaa tcattacaaa ggggaataaa
     2941 ccaatcagaa tggcctcagc agcagaacga atcaagggaa gattccaact tgccattgat
     3001 gagccggttt tttgggccgc aatatactga ttacgagctt gaacttgagc aaatgcctgc
     3061 tgtacggcag ccggatttcg gttgcgttgg ccattgataa tgcctgcttg acgaataacc
     3121 tcaaccatcg ccgcctgttc gatatttttc aatgtatcga agcgggcatt atccagcaga
     3181 tattggaatg agctattgcc taaggtaacc agggcattta cccggtttat tcctgccgaa
     3241 tttgtaccca aacctgcaaa aacacgatcg gcaatgtagc cgggcaagta atttttgcta
     3301 taaaaccggg tacggtcttt tagttctttt gccgcctcat tgcaggtcat ggtctttgat
     3361 ttgccgttat actgcaacat gttgcctctt cttgaggcga tctgcgatac attggtaaac
     3421 aaggcttggt tggtatgggt caatgtagcc agaatatcgg cactctgtgc cagatcctgc
     3481 acgttataga agtgatacag gttcatatca aagaatgaac actgatacat gaacagtgcc
     3541 cagtcttgag tcagttgcgc gtccgggatt cgtgcttcac ggatattttc catcaggcgc
     3601 gttccgaaca gcatgccggt gcgtgagtaa ttgaggtcgt ccggcaggtg gttaacctgc
     3661 tcggctttgc gggtaatact ttggccaaaa gaactgacaa agtagcccat ataagcaagg
     3721 ccgactggta cattgccgac agtatattga cggcctgtat atccataccc acctgattca
     3781 gtaatgagta cagtggtttt agggaccatc atacccatcc aacaaaataa cacgacaaac
     3841 aaataactga agttatcctt caaatccaat ttgaagactg caacagcaag agtgaccagg
     3901 aatccaaata aggcagcagc ctgcattgcg ccacccatca tcgtagatcc tgatattgtg
     3961 gcaacgccat tgaaaaccgc ccgtacagct tctccgttac cagggaaagt aaagtattcg
     4021 acagccatca tctctcctta gaaacggttc atcgaatcaa aagtcatgga gttgttggta
     4081 tccacaaagg ctgagcgcca ttgtgtttca aactgctcca tagccctcat catgctttct
     4141 tcgatctcga ttttcttcat tgagtcaatg cgctcctggt aaattctgct tcgcaattcg
     4201 ccaaggttgg caaacaattt tcgaaccgct tccccgctgg catcgtctgt cttgttatac
     4261 atacccaagg tcgagcgtac ctggcgttcc gattggtcca aaatattggc cataatatca
     4321 acggcaatcg catctaaaac cgctgttttt ttcaaatagc cagttccaag gaaagcatct
     4381 gatacagcca ttttcagtat gggcaattta ctgttattga caatggcagc aatctgcttg
     4441 cgcgtagata aattccaacc ttggttattc cttattgcag tcggaagcgt ttcaatgcgt
     4501 ttgtgaacca tgtaccgcaa agaaggtatt tctacatcat ttttctcagt gcagttctcc
     4561 attaatttct tagaaccgtc tttgcacgtc cacatatcca atacaacctt ctcccggatt
     4621 tctcccccgg cattgttttt tccgtttaaa agatcttgga tgctgaggat ttttggttcg
     4681 aaactttgaa tacctactgt gtatttgtcc ttatcccctt catgttgtgt taatggagtg
     4741 aacacaattg ttcccagcat gctgtaatag aagtccatgt cgatacccag tttcgagcgt
     4801 tgacccgtat agttcttcat ggcctcataa gtcaaattac cgccggtaaa gctaatcgta
     4861 tcttggggtt ttccggtgtt tcgcacctta tcggcttcat ggacaaggtt tttagcggcc
     4921 tgacagtacc aagcaccttc tgccggatca tttgacgaac ccgaatccac tgaagcggcg
     4981 cgacaatttg aactggccaa cgaacccagt ttccccattg cgccgccgac aatatcctga
     5041 ctcagtttgc aactgtttaa actgttttga ttaacataat tgaccaaatc acgaataccg
     5101 ccaattgcat cttggatcat cggatcaagg gccttaagtg ccaggtcaaa ggctacacct
     5161 gctgcgttat tgcccaggtt tcgcgtaaat tggatgaatt gttctttatt gacaaacgag
     5221 aaagagccgg caaagaaatc tataccgccg caaccggcac tgattgaagg taattgaata
     5281 ttcccaaggc tgatatccga caccggaaag cgaacgctca taccgcctcc cgtatatagg
     5341 ctgccgcttt gaccgtggaa cgcacctgct tcagtataat tggcttccgc tcccattgag
     5401 ttaaacatgt cagccatgtt cttctctatg ccggcactac tattgatcgg aagggccagc
     5461 gatgccgcca agcatagata ttttgcagag gaagcacaaa ctgtcggcac tttggccggc
     5521 ttcggaaatc gaaagatgtg aaatttcata accgctccat gaagcggtta tagcagggaa
     5581 atccttacta taaccaagat aagttacagt aaagacttga gattaaggga atcttatttt
     5641 acctgaaata tccgcgttgt caaccggtta atacatagaa atataaatat tgaaggcccc
     5701 gtatagggcc tttgatttga ttaatacaat gtcccgacag ggcgggaata cgctaagaaa
     5761 cgatcctcaa gcgtggtttg cgccatcacc ccaaatccaa tgggtgtaaa ttgcttggtt
     5821 tttgtatcca tgacgtacat tgcaggtaca gtagtaatat tcaacctttg ggcaataccg
     5881 ttgttcggca aagcacgcgg gaagtgatta atacctccgc catcgagact gaccggcata
     5941 accttaatac cgtattggtc ggcaaagttt ttcaaaatcg gcgccatggt atgacagtaa
     6001 gggcaatccg atttgaaaac aaacataatg ccgttctctc cggcaagttt tctaattgcc
     6061 ccggcctttt gttgctgacg gttatgatca tattgttgct gggccagttg gttgttcgga
     6121 cgaccgcgct ggctgtaatc caaatttggg ttttgccaaa ttaccctttg ccattgatcg
     6181 gcaaatgccg cagatttgtt catcacaacc tcttgaagtt caatatatcg cttcaaattt
     6241 tgaggtgtgg ggttcatgac ggcaatttta cgcgactcat ccaaatttct ttgcagcatt
     6301 tcaaattctg caagttcatt acgaaccgga gtattttgag gccggttttg tccggcttgt
     6361 ttgggctttt tagcatccgg ataccataag aaagcacggt tttcgttttt ccagatatct
     6421 tggtcatacc atcccatgcc atcatccgca gctcgcagat tggaggcgtg caggctaaat
     6481 atcagcatga cggccatccg cactcttttc attttttacc accgttcttt ctgttcgggc
     6541 acgccccgat atccataatt tcagacggaa aggtttgcga cagtgcgggc gaagtatgga
     6601 atgtcaagcg aaccctgttg catccgggtt tgtttttaat ttcttccatt ctgaccgcct
     6661 cgacaaaaat aggtatgtcc tgccggaatt tctgtttgac ggcagccgca gcacggccgc
     6721 tgaaataccc ttttgccgaa ccgtgacgca ttgccagcgc aagcaaggca tccggcttgc
     6781 cgtcaggaag cgggatggaa gtccttgcta gagcttctgt tttttccaaa gcgataccgc
     6841 cggtataagg aagggatgcg gcaagtgcca tacctgtaaa aaacataccc gatacaatac
     6901 ccgccgacgc agccaaccaa ttattgatat tcataatagt tcttcacttc caaatttttc
     6961 ccattaacgg atgcctccct gattttttgt gaaacctttt gcccctgctt ggccacgttg
     7021 tccatgttgg catagatttc ttcataaaac ccggacagat caagtttgga aaagtctaac
     7081 tgttccaatt cctgtgcggt aaatccttcg catttcgggt tctccggcgt accccaaccc
     7141 cgtttcaatt gctctttgcc gccgacattg actattttgg ctaatttgga aatgaaacag
     7201 cactggctct ctatcattgt tacacatgcc cccaatattt tgctgccgca ataagaaccg
     7261 acataatggc acaaatctgc tttacgcttc atggcaacta aaatttcact ttgcgggcac
     7321 ttcaaccact gttggatggc gattacggca atagcagccg caaatacggc cgggttgaag
     7381 ccgacaatta cccccatacc ctgttgtccc gcagcagaga aacccaacgc accaatctga
     7441 gatgttccgg taataccgcc ggccgcactc agggccgtac ccattgcctg tgctgcggag
     7501 ctgacaaaca tagtggtaaa cgtataatgg ctggcaacgc tggccataaa atacgttgtt
     7561 gccatagttg tccctgtctg taccgcgaag tcaacaaatt tgcccggatc tgcttttgtt
     7621 cgacagcagt ctccgccgaa tacgctgtct aaagcaccat ccagcttgat agagcattgc
     7681 ccggcttctc cattgaaaaa acgctgtttt tcgggatcat aaaaccgttg cgtctcacgg
     7741 gcgaattcca tcgtcgtcat agttttggca aaatcccgat cctgacaacc ggatacctct
     7801 ttggttgtct ctcctttttc ggtacattca aactgttttt ctctggcttg acaggtaccg
     7861 tccggcagct gatgcgtaca cttgtcccca atcagacggc attggtctga ggatgtataa
     7921 gcgctgcatg tttcggaatc gcttccgtcc gtacattgca cggtgcgcct ttctttccag
     7981 caatcacgtg taacgggtac gccgttgatg gtcttggtac tgccgggttc caaacactct
     8041 gttgcaactg ctttacattt ttttccttgt acctgacatg acgactcatc ccattgcgaa
     8101 ctaatcagat gggtatgctc catttcttcg actttggtcg gaagccttcc ttgatgtaac
     8161 gttctcaaat ccacatcgca agaatacttg tgcgtgtaac gcaaacactc cccctcattc
     8221 gactgctcca aacaggtttg tccctgtact tcacattttg ccgaagggtc ttttaacggt
     8281 gcgcaataat cggcagaatc cttctcaagg cactgatact cttctttgta ttcccaacag
     8341 tctttagtta cgtcgacccc gttgatgttt ttggtcgacg ggccatcaac acaaacggaa
     8401 gacgtccggg tacacgctgc actttccctg agtgcggcag cagacgctga agtgattaac
     8461 ataaagtggc agatcataac cagcaaagac gttatagccc tcattgtttt accctcttct
     8521 catactccga acaactgttt tcccatttat cgtgacaaac gggctcgcag tattggtgta
     8581 cattaaagaa gatagcattt tctccgccgc ctccgacaac ggtgcgggtc caaatggtat
     8641 ttttgccctg atgcagatac ggccgtatgt cgatgttcag gtttttcctc caggatttac
     8701 ctctttcagc gttacccaac cgagtacctt cctcattacg taccggtgca aaccttcttc
     8761 ctataggaaa tccatcttca tcttttgcag tataatctgc cgcaaacatc tgattgttat
     8821 agctgctgta cacaattttg ccgttgactt tgacaatcag ccaatcatcg tactcaaccc
     8881 ggctcaaagt aaacagcgtc agtttttccg gatttttcac attgagcgtc atctcccgat
     8941 catactgccc gtcacgccaa tagttgtcgc caatagtacc gaacatcagc ctgtgtgcgc
     9001 ttcccgaagc cgtcagcctg accgtcatat caccctgcag gctgcctgct tctatacccg
     9061 aacgggtaca gtttggtcct gatttctcgc ataccacctt taatttcttg ccgcattgat
     9121 actgctggtt tcgatatttg ctctccaggc aggaatagtt ccagccggga tcgacattga
     9181 ccttaagggc cgcctcgcac aagaacatct ctgcgccatg gtattcattg caacgtttga
     9241 cggtttttac cgcaggaacg gtaatggttt ccgtaggaca tgccgacgag ggaactgtgc
     9301 cgacgctgcc gtcttcattg acgggatatt tccatttgta tttttccagt gtttctgccg
     9361 gatcgcctgc aattttctgc gtgcctttaa ccaacttttc atttgaagat atggttacat
     9421 cgggccgctt ggtacggctt cctgcaatca ggtttacacc ttcgcattct tgacgaagat
     9481 agagatccgg attggcaggc agatttttac agtcgttgaa tttggcgtca ccaaactgag
     9541 tcggaagaga aactccgcca ccgtaatact gttcagccat tggtacgttg tctgtatatc
     9601 ttggggtata ttgggcaggc tgcatcatgt ttgttgcagt cccggccccc aattttccca
     9661 gttcgccgac gtaactaccg taatctgcag caaaggccgt ctgcatgagg ccaaatacag
     9721 ttagataaat ggcatgtctt ttcattttct gccttccaga acggctaatt tcgcatttgc
     9781 aatatcggaa agttttttat cttcagactc acggatcaag tgttccaaag ccgaatgcaa
     9841 ggacacatca ccctttacgg caacatgttc cgtacacatc cccaattctg aatttacagc
     9901 gcaaccgtct aaaccatctt cgctgtttcg ggcgattacc gtaacaggga cttcggtgat
     9961 gccgtaatgg tcgaagagtt cgggatgaat cagcatatcg ccttgcaaag ccgcaagttc
    10021 ttcggccgcc ttaaccgtgg ctttcagtga gtcgtcttta aacccgcgga taaccattac
    10081 cccgcctgcc tttgctgttt cacgggcaat gcgtttcaag ctggcttcgg gcattgaaaa
    10141 tgatacgaat accattaaat ccgctttttc cgcagcctgt cgggcttctt gtgaggcagt
    10201 gcggtatttt tgtgcgatag acatcggatc agccttgatt tttgcatttc cgtaatcacg
    10261 aacatcgggc acacggtgct tgataccttt caaatccgga atttcaggtt gctgccgatt
    10321 gtcacccaca tttgggtttt taactcgggt aactttaccc atggttgtac gcagacggtt
    10381 tcgcgccgcc tctacatccg catcggcgta agcactgcct actccggcca aaccgcccaa
    10441 aagaatgagg aagacgcctc cggctacgat tacattgcgt aaagacttgt ccatcactcc
    10501 atctcctgtc aattagtctt aaatttatat gcaccgcagc agtcgcgctt acggaaaagc
    10561 tggaaagcaa actgattacc tttgtatgga aatgctttgc cgctgtctct gactaatgtt
    10621 gaacgtccaa taggctcaca gcaacggcct ctgaattttc gggcggccac cggataggtc
    10681 atagccattt tatagttctg tttatccatt acgggattaa gtttgtaccc gcataaagca
    10741 tccctgcctg atgtcctcca agctactaag tccctgtgca tttttgtctg aattctagcc
    10801 gccatcaatt tagcggcctg cacttcggag aagtggccgg acatccatcc tgacaaaggg
    10861 taaatcgttc cgtggcaacc tgcacaccaa tacaatttat tgaatggaaa gccggcggcc
    10921 gctttaacac agtcagccgc acaggctgct tgtgcaacga cattggcaaa gagcgcagaa
    10981 tcaggggctt gataaacggc caatgacgga tatttccaag tgggatcaag ttccgtcatc
    11041 caaccgatat ccaaagtacc tttttccata caatcgttgt ccaataaggc ttccaaccaa
    11101 aatacgaccg gatttttata ccaatgaact tgataaaagg cattatgctg atcatgttgt
    11161 tcgttacgac cacgcttatg cgagccgcca aaaagatttt gaatcttatt ggatacgtct
    11221 gctttacctg ttgtttccgc cgtatctgct ttttgatttt taagtgaaat accgcctagg
    11281 ctaaccatac agaagggggt gcgtacgact tctaccaagt gtgtgggttc ccaaaatgat
    11341 accgtcacgc ctgaacggct cgggtgcgta cacgaacaaa accaattgtt tggagaagag
    11401 ccgttatcct cttgattatc catattcaga ttgattttgc cgccgatttt gattgggaac
    11461 atacagctcc aacaaatatc atttatcgga ttaagcatct tcccctggca ggttaattta
    11521 ttcggtatcg gagcagtagg aacagcttca gcggcgtgag atatagaaaa taatgggagt
    11581 agtaataacg gaatcagtaa tttcagcggt gtgacaaata ttttcacggt ttcatgacct
    11641 ctacacggag ccggccccct tcttgggaga ccaaacttgg aacttcattc agctgaaaac
    11701 ttttggacat ccgcccttct tggtcgtaat aaaccgcctg atccatttct actgataact
    11761 cataccaatt gcctgcagtc agaataggca tgaatgattc gccatattct gctgcaagtt
    11821 tcttggccag ttcgatttgg tcaggttccc ggccgtcaaa gaaaaacatt ttgaatggga
    11881 ttggggctgc ttccaaaggt ttgacaacag taccggcagg agcaataatt tgaccgtctt
    11941 ggtcataaat tgtttcggtt aaggtataac ttgggtcgaa gtatcgaaca gagcctttag
    12001 ttgccttcgc cagtccttcc aagggctgta gatttaaggc ggagcgattt attctatctt
    12061 ggacctcttt ctgcattgcc gccaattggc cgctgtcttg tttagctttc aaacgcgctt
    12121 caatttcgcg gattgcatgt ttttccgcta ttgggtatgt ggtgccgatt ttgtcgccaa
    12181 ccttctcgac agagggtgtc gaagcggcta cccatgagga aattccggtt gcaagtatta
    12241 ctagcgatgc gactgaagct atttttttct tgtgggacat actcactcct aaaaaatggg
    12301 gaccgcctta ccaataattt gccctgaatg gattaagccg gataattcat atcgactgtc
    12361 caagctgtct ttgtgccctg ttttaaccca catgaaccgt tgtggaattt ccccttcgaa
    12421 tgggttgatg gccagcttca tgccatccag ggagtattct tttgcagtac ctacctcttt
    12481 cccatctatg aaaaaagtac gattcgcctt cgttacatga tttccgggaa gtccggcaac
    12541 ctctttgacg acctgcgtac caatagggta aaaaccacct tgccatgcaa aggccacata
    12601 atcgccctgc ttcagatcac ttagcttgtt cgcatctttt ttgatgaagt aaagatggtg
    12661 aggcaaggaa cgggaaagat taaatgccaa agcataatgt tgattgaatg ccgacaagca
    12721 gaccgcaact gctaaaaaag gggttgcata tttctgacaa aactccctat aaagacgttt
    12781 catcactgct ttgttgcttt ctttttgtga tatttttatc attgcctcaa acattaacga
    12841 tccatttctt cttcagtaac cggggtgcct ttaccctctt cggccatcgg tttcggaatt
    12901 gtcttttcaa cttgttgatg taccggtgcc gccactacag ctgtggccgg gacacgctta
    12961 tccaactctt tcataatgtc agcagtaaca tcaaccacct ctgtgttccg tccgaccaat
    13021 gcagatttga caaagatgtt acagccgcac cgaattacct tttcttcgat aacctgttgc
    13081 aaggtattca tctttgcttg gtagttttgg aggatcgcgg ctcgttgccg gtcggaaatt
    13141 ccattctgca gtccttgatt cttcgcctgt tcccggtaag tcatggagat accggccaaa
    13201 tctacaatat ggaccggttt ctcatgggtt ttcatgaaat tccatacgcc taatgccaat
    13261 gcggcaatag aaattgcgcc gatcacaagg ctgcccaatg aaacctctac gccgttatcc
    13321 tccggattct tttccggatc ggtatttgcc gtgttttctt tcttttcttt tgcttcagtc
    13381 atcttattaa tatcctatcc tctgcctaat tagcgggttg cggcacggtc ccttaaaact
    13441 gcttcaatgg cctcggctgt attcaacccc tgatttctgt aatggttcac ggcttcaacg
    13501 tcttccgctt ttgagcttga ggcaagaacg ttgaatgggt ccgagtacaa cctacctact
    13561 ccataaccta tcggggtaga gacaaatatt tcactgtagt agtcagtttt gctcaaagtt
    13621 cgcagaatct gcataatccc gtcattgact tcaaaaggtt ctttgtcttg aatggttttg
    13681 agcgatttag ccccggtacg caattggaaa acaaagtcgg cattctctaa tgccgcctca
    13741 gccgccttag tcttgtagta gtcatttgca ccttgcgtca gagtgccgca tgcacctttg
    13801 tacttacgga cacggcggta gccttcctca ataaaacgag cggctgtacc cgtgtcgccc
    13861 aacaaagccc aagcctcgtc aatcatcata attttgtact ggcttcgtga taggtacatg
    13921 ccctgcataa tgcggtacat aatcagctgc atgatgatct gttgcagatc cggtttggtt
    13981 tttaactctt caagttccag tacgaccagg tcatttttaa actcgacatt caattttcca
    14041 ttgaagtaac tgccgtagat accttccgaa cagtatgtgt acaattggtc ggcaacggcc
    14101 accatagaca aatctcgttc accgggcttt cctccttcat tactgccggt aatcaaacac
    14161 ttgagacagc gttcccttac atgatcgacc gtgccttctt gtccgaattc gtaccatact
    14221 tcttcgatta tccgttgaag cttggcttcc tgctgttccg ttagggagtc tgacggagag
    14281 gccatcaatg agaagattag tttcaacatc tcaatatctt cgttgatgtc cgttaccatt
    14341 tggaatggcg ggaagcacag gcctgcttca taggtaaatt caatatactg cccgccaatc
    14401 atttggcaga atttttcata cgaacggcca acgtcgataa tccatacctg agtccccagc
    14461 cccatgtagt tgcgcacaat ttcattggca aaaaatgatt tgccggaacc ggaagcgcca
    14521 ataatggcaa agttatagtt acctgccggg ttggcaaata agtcgattcc catggcctgc
    14581 ccgcgttttc cgaacaaacc gatgacaggt cttcctaaac cgtgccactc gccgataacg
    14641 ggagacatac agacagcatt agtcatggtt ttggttgacc atcttttctt actgtccaaa
    14701 tatttctcaa cctctttgtc gatagccatt gggagggcag tcaaatatga ccccagttga
    14761 agatattggt cacgataaag accgaagccc tgagcctgcc aaatcgatac cgcctggctt
    14821 tcagccaagt taatgttttc gggacgctcc caaatgacaa cttggtgcaa caaactcacc
    14881 agaccgccgc cgcctttgcc gaacgcttct aggcatatgt cgtaatcacg tttgattttt
    14941 gctgcctccg gcataactgc ggccatcttg gattccgccg tttgtttggc gcgggccgct
    15001 ttcagtttta cggtattgct ggaagtgtca taatcacgct tgaacatcgc catggaaatc
    15061 agaaatggtg atgtgtaatt catgtttgcc tcaatgacat caccaatcaa tgcgcccata
    15121 ttggacagat gaaactcttc agggtagcga ttggcagaat agcattgagc aatcacgcaa
    15181 tcccccgcgg cacggttgcc aaaacgcagc cccgagccgc tatcaagaac cttgatagat
    15241 gtgcgggtct caatcatttg ttcactcaag aacttggttt catcccaaac aggatcgatt
    15301 tcatcttgtt gtccggtaaa catcctatta gggttgagta ggagcgttgt ccaagccaat
    15361 aaatcgtctg cattccaagt tcggccgtaa atctgcatgg agcggagtga atcgcgcatg
    15421 cgggcaagga gcgaaaccgc cttgctgact tccactgcat tatcaggtgg aatattcaaa
    15481 tttacgctga ggatacagcg cagatttcgt aaacgaatgg ccgtattttc taccaagacc
    15541 ttctgtgtac ccttattcca aaattcagta cgttttttgg ccaaagtttc gaaagtgctt
    15601 cgcatcggat cgtctatagg tcgtgatgct tgtacttgct catattcctt gaagtaattt
    15661 cttaaatcgg gggagccgaa aatttgaacc tgtatcggcg tcttttcagg aagcagcgca
    15721 tacaggctgc cccacttacc gatcaggtcg tcactgccgc ctaaaaatgg gttaagttcc
    15781 agaataaagc ccatgcccaa cggtttgtcg cctttaacgg acatcagcgt aaacaactct
    15841 gttttggtat cgtatgcacc gtaattgaga tagtcgctca aatggtcaaa gtcaggatcg
    15901 acagggttgt tgtgcaaggc ttccatcggt tgatcgacat catccgaacc ggccaaccaa
    15961 tttttaaatt ttgacaggac gcccattaca tacctctatc gaacggtacc gggaataccc
    16021 gatgtttcct gcagatgaag attcacgcca acatcgggga tgcggtcatt tgtgttgttt
    16081 gaattggttg gagaagtctc aaccgaactg cctgaagttt tggccccatc ccccaataag
    16141 cggatgggac gatattcatt tataatctgt tgcccgttgt gggcaattaa ccaatcaccc
    16201 tgattcagaa cgacatatgt gaaactttgg tcatttaaat cgccgtccgt atcaagatag
    16261 ggagcgaccc aaatgcgagc aatttcactt tgcgagcgaa cgggcgtacc ggaggaataa
    16321 gcttgcgtta acattaatgt gcgaacactt tgatcagtgt aaggttgtcc aacctgtctt
    16381 ttcataccgt cttttaaaac acctgcaaca gatgctttat agttactgct tatagattca
    16441 caataaggat cgtcactgtt ccgggcatta gggcatcgga atttttcact accgccgata
    16501 ccggacatgg ttaaggttga acatccgcta agaatcaata ttgccgcagt aatcgataag
    16561 gctttcaaac gcatcattct gctcccgctc catttaacca tttttccaat tcggcacgcg
    16621 gcatggcacc ggatgtacgc cgaccatctt tatgaatcat gcttggagta ccgcgaactt
    16681 taagctgctc tgcgagtgca atattttctg atacagggtt tttacagtta ccggccgccg
    16741 gttctttatc catcagcatg tagttattcc atgctttttc acgatcttta ctgcaccaga
    16801 tatgttctgc ttttgaaatt gcatcagggt ggagggattt aatcgggaac atgaaaacgt
    16861 atgcggtata gtctgtcatg ccggccaatg tttcttccaa acgacgacag aacgggcaat
    16921 ccgggtcgga aaaaagtgca aattctcgct tgcctttgcc gccattacct ttaacgattt
    16981 tgatggcgtt tttgaatggc agtcgggaat aactcttttg tgttaccgct gaacgggcca
    17041 tctcggtcaa gtctttctgc tcttgcatat cgtacaacgc gccaaaaatg aaatagcgac
    17101 cgttttcttg tacataggca atgttgtcac ccattgtcac ttcataaatt gaagccatgg
    17161 gggtagcctt tactgaagaa aacgtggtat tcggatacaa cttgtgcaga gtgcttccaa
    17221 cagttttgac atcggcaaag gccgtctgaa aagctgtgat tgctgcaata gtggccaaga
    17281 ttttcttttt catagatgga tcctatttgg tttcgccggt atcaatttcg ataccttgag
    17341 tcagtacgac atcgacttga cggcctgcat tgacttcaac taccgggaag accttatcgg
    17401 ccagttttat gtaatactca gcaagcctgt cagctgcacc accgatacca gatgcaatcc
    17461 ctgcacgata ttgatcactg ccccgaatag aggttgtgct gccaaatgcg gtattattcg
    17521 tcaccttgaa tgcttccgat acgcccttac ccataccgct aatcacgccg gacatcagtg
    17581 catttgccaa gagttggcct tgtttcgtta caagattacc tctcacgcct gttttgccgt
    17641 cttcaccggc aacgtaacct tttaccggca tgtcgatggc tcggccatcg gtacctacac
    17701 agctgagatt ctgcagtctg atgtaagcgc gttctgaaga tatatcgccg taagcctgcc
    17761 cggttacgaa acattctctg aaattgtatt taaaccggtt gggaagttgc gcaaaatccg
    17821 ttacgcgcat taaaacaggg tgggggctgg cattttgtgc ctcacccccg gtcggtgcgt
    17881 caaccccacc taacagtacg gatcggaaaa aagtaccgct aggtatatac gagtcacgcg
    17941 cccgaaactt gccttgcgag acgacaacct taccgccgtc ttcaactact tctatattca
    18001 tacgcggggt ttttgattta acccactccg cttcttttaa ttgcggggaa acccgtacat
    18061 tttcattcgg atttccggta tttaccgatc cggtcgttcc aggttgcagc tctgcttcac
    18121 tcatacttcc tgaaacgatt ttccgttcaa tgggcctatc tgccggaggc gccccgttcg
    18181 gcaatgtgcc tgccggaggg gccggaggaa gccggccgtt atccccgata ccgctaattg
    18241 caggcggttt gcctaatcca tcaggcccca gccccttact tgtatcagga ttactgctgt
    18301 tctctttttc ttttaactcc tgaaccatgg tttccaattc gctgattttt cgatttgcat
    18361 attcaatctt cgaactttct gtgttcatcc ataaggaatt gtccgtaatg cctgcttggg
    18421 ggcttgtaaa atcagttctg agaatctgcc gtttgttttg aacctgtgtg acttcggcag
    18481 atttcttttt attgccttca atgacataaa ttgcactgcc cacaatcgcc gccatgctgc
    18541 ccgcaaccat gcccgcgatg gctaactggc gttttcgcgc tgctgaaagt ttaaatttgt
    18601 ttactttcac cctcatctca acctcccgga cggctgataa taaatacgtc ggtaatttcc
    18661 ccgggttgta ggatctgttt ccgaaccgct accgccaaaa ctccttgtcg gtaaaattcc
    18721 tgctctctaa tttcaagttg tttagtacct aaattagtta atgtaaaaga aaggccttgc
    18781 atatcagcgg cggtgtaact tccgttttga ataaagaggg tgtttttcca taatgggacg
    18841 gtttgatatt catgactatg attgatgccc atatcgccgg tagtgccacg catcatagag
    18901 gccatcattt ttttgatggc ccgtacatat tctgtactat tcatcgttac aggcattgga
    18961 ccggtttgcg aagccaatac tgctgcttca cggcgattag ctccagccac atcgattacg
    19021 atgttgtcac cctgttttga tgtaggttta agaaccagca aataggtctt accttcttcg
    19081 cttataacga aaagatttgt cggattggag gtcaaactac ggacaaacag ttggcctgtt
    19141 gttgtatctt tctgaagctc aagatcaccc tccataaatt tccatgatga aattcgaccg
    19201 ccttcaatgg cgattctgct caagttccgt ttacttattg aaacaacgtg aggtgtattt
    19261 tctgttgccg gcactcgttg cgctgcaaaa ctagtcgcgg gtccggctgc caacaaggca
    19321 gaaaagacaa ttttttttaa cagtgaacac atatcaatta cctcgattac cgcctttaaa
    19381 ttctgttgcc gttttactga tttctacatc ctcggtttca acttcggatt tttcctgacc
    19441 tgcgggttct gctttagtta cacgcttttg aacttcttta atcgataata attgcagtgt
    19501 tccataatta tttttgaaag aaataagtag ctcgcggtct ttttgctcta ccagggcatt
    19561 gttgcgttta acctcaaaaa ccccacgaat ggaaactgta ttgtctgaaa tgtccgccgt
    19621 tgcttctgtc gcgctaaaag tggtgccgac attatttttc ttaacccata aggcatttaa
    19681 agacagctcc ctctctaggg catctttata atcaggtgaa acaatcttca ataattgact
    19741 attctgataa tcgactgttt taggggttac atttaagaga agataggcag catagggagc
    19801 catttcttca agatattctt tagaaaaagc gatgtttgat acagtcattg accgccggat
    19861 ttccggcggg gtcagcgtcg tttgaacaat cgtacctttg aagagcaggg tcgcggacaa
    19921 aaatagattg acagctatag acattcccaa aagtatccaa aaaaagacct ttatgccggt
    19981 tcttatcgcc aattcactca acatatcttc agtcttcaat ttagaaactc ccgtttattc
    20041 gtttcaggta tccgtttgtt tttttcatta atgggtaagt accagtaaag aacatgcaaa
    20101 aaaaaccatt gatgcttgcc agacttaagc ttgccccacc tccatgcaat gaaaaggcta
    20161 attgacatgg aaatgaaaaa gtgacgagag acaataccta taccgaagcc ggacataaat
    20221 aatataaaaa cgtccaagtc ccaaataaat gcctttttgg gatcgtctat cgataccggc
    20281 acatgataat ctctcatcac ttctcttatc tagtttgatt aaataatcaa ggtaaacata
    20341 cctttaatga tgcccgggcc taatgttgcg gccaagccaa cacccaaacc gacgatacat
    20401 ggcatgatgg tttgtttcat caggcctacc gccaaaccga tcaagaatgc agtcaagccc
    20461 aaagccatac ccagataacc ttcagaccag cctgttacca tgtcagccaa gcctttgaat
    20521 tcggcacctg ttgtagcggc cattgccaag ttagcataca atgccaatgc aaccgcacta
    20581 gcggacttta ctaatttatt gcgtttgata ttcattatga acctcattaa acagagctta
    20641 tatcgccaag cttttgcgtc cgatcattct ttaccaagtg attacaacgg ttctcgtatc
    20701 atcactattg cagcaggccg gggaacttgc tgttcttatt tttgcggata cattatgttt
    20761 cctcagccat ttacctatta ctgctgcccg ttgtttagcc aaacgtctat taattgcagt
    20821 atttcgacct ccgatactgt cggcccatcc gcgtaaataa accgtctgag tatttttcaa
    20881 tttaggcaga acacgtttga gcgttctcac gccctccgca ccgaggtgcg aactgcctaa
    20941 attgaaaaac accctgatct catctttttg aagattggtt tgtgtttgag cataaaccgg
    21001 ttgttccgcc aaaaccttgc gctcaaatcc cgaacactct tcattgcggc agaagacaaa
    21061 ttcgcccagc tgattttgtt ccactgtata agttgcgtct tgaggcagcc cggccacttc
    21121 ctgttttggg gcggcacagg ctgtcagaat cattgcggag gctacgaaga gcgtgttttt
    21181 aatcgtttcc aattttgata taccttattt gcgtaagtca ttcttgcgcg atagcacgcc
    21241 cggcctttaa gtttggtaca tgcggcgtta taagcaccaa ctgcttccca tgatttccca
    21301 tgttgccgat aactatttgc caatatccat gcaccaatat gtacatttgt gcatggattc
    21361 catacatccg cccctgttaa accaaatttc ttgtggagta cgggtagcca tgaacggttg
    21421 atacccatca agccaatatc agctgtatga ctttcaatat tcggcctgaa agcgctctcg
    21481 gttaatgcga tcgattgcaa catggcggga tcgatgccat atttgctgcc ggcttcctta
    21541 taacaaaaag caactgctct tgcagatgtg agtaccaaaa cacccggtac gacgaactgt
    21601 aaaagtatgg cccttaatct catgtcttta ctgtcgttgt atgtatgact aatttttatc
    21661 tcgattcgac aatttttgtt aaatcaaacg aaattaacta aattttttag aaatgtttct
    21721 ttaaagatag attatggtat aattaaaagt tacgcttata ttttatgaaa ttaccgattc
    21781 ataacatatt gatataaaaa ggaatacgat aagggtaaag gctcttatcc aaaagccaaa
    21841 aggccgtctg ctaaaaaaaa tcgcagccgg ccttttttaa aagctgtaac ggtaaagcaa
    21901 cagccttaat ggttaaagga agggttcaag ggaaaaaggg taaaagttaa aggtaaaaca
    21961 aaggtctgca tgtaaaaatg caggcctttg cctattaaaa ggggcagcat tataccttat
    22021 aaatcttatt ctgtgacaat tctaattaaa ataacatttt ttattttgat ttttgacatg
    22081 gcaacttttt tttattatat gaatggtttg acaaccctgt ctttttaatc aaaatgaatg
    22141 aaacagatct tcccaagctt cttagtgtca taaatcggtt tacggatatc gataaaaact
    22201 ggtcttgcgt gttgttgttc tggatacagc gtgctagcgg ttatctggaa atgatgacgc
    22261 tggatgacga tgaaaatacg gcaaccttcc ttatcgaaaa actagagaaa ctactagagc
    22321 cactgccgat agatattgat aacacgactt ttgcagaagc acttgccgat gactttgaaa
    22381 tcttatttct aatggcccaa tacggttctg aatactggaa ttcacttgaa gaaagctttg
    22441 aggaattttg tattagacat actacgctac ctaatcaatt gattgctgat attccacatg
    22501 caaaaaagga aaaagtcacc atgtggctaa aatctttgct taaatcatca tgaaaacaag
    22561 ccttctcact attgcatcct cactaatgct tgtcagcggc tcaatgctat acctgataaa
    22621 tacgagaaaa agtacagatt ccgtgtcaca taatgatgag gttgcccata tcaatcagaa
    22681 catagtaaat gaccggtttc gcattttaaa cgcgcatgaa ctgattcagg tgctcgactt
    22741 aagccctcaa atatccggta ttaagatgaa cttagggtta tcggatgaaa actggagtaa
    22801 ggatgccctt ccctttttgg aaaaatatat agcctttgtt caaaggcttc ccgccagtga
    22861 gtctcatcac catgcaggtg atggtggttt agtccgccat acactcgatg tggctgcact
    22921 ggctcttgtg gcatctacat cgcaatcatg gcccccaaat gccaaaaccg aggaaatagc
    22981 gaagaaaact gccgtttggc gttatggaat catgtgtgcc gccctgcttc acgacgttgg
    23041 taaaactgtg acgggatttc aggttgaact gtttgattcg gctatcagtc tcgaaaaatt
    23101 attatggttg cctgatacag gctccatggc tgaaagtggc aagctttatt atcgtgtcga
    23161 atttcctgat gcaaagtctg cttacagtac acatgccgaa atcgcctgga cattctttca
    23221 agctttggtt ccttcacatg tcagacaatg gcttgccaca acggatccga atttaatgat
    23281 tactttgaga aattatctga gtgggaaaaa ggatggcagt cctcttgagc agttaataaa
    23341 aaatgcggat atgacctcag tttcaaggga tttacgaagc ggcagccgtc aacgcttctc
    23401 aaccgccaag cgtaaaccat tcattgaaac cattatggaa acattgaagg agatgttatc
    23461 cgatagagga gtccatttca gcatcgcaac aactgccggt ggtgacctgt tccgtaaggg
    23521 tgatcgcatc tatttgatgt caaaaaacgt acctgactac atccggcagt atttgaggaa
    23581 aaatcaacat cctgctgcag gctcatttcc ggctgacaac caaaggatat ttgacacatt
    23641 attcgagtac cgtgccgtca ttccgccgga aaacgatcct cacagggcaa ttaaccatat
    23701 agaggttgag tttacccgta tggacggtga aaaggtccgt aatatcttca gtgtactttg
    23761 tttcaatgcc aaaaccctgt atccggacgg agattatccg actgagttct taggtaatct
    23821 ttcagaagtt tccgccaaga aggaaatacc ggatgttgta gtatcagagg ataaagtgtc
    23881 aactcagata caaggtgccg aagataaccc agtcactatg accaaggaca aaatggactt
    23941 cctgtctatc caaccggtga cagcagaaca acctccccta cctgaaaaaa atcaaactga
    24001 gacagctaac tcggcttttg acaacagtat gaatactgct gctccagcgg aaaataaatc
    24061 tagcgagtca gagaaagcta aagaaccggg ttacggcaca attgataatt taattgaaaa
    24121 ctttaacctg atagaagctg attcagaaca aacagaatca accaaatcag aagccgagac
    24181 agcagaaaat gccgttaacc tggttcaaga aaatataaga gtagaagtta aaactgagac
    24241 agtggacaaa cctcgcgctg aggttaaaga aactgagaca gataaagcac caaaaacaat
    24301 taaatcagga accaaagcaa gccgtaagaa acttgccggc cttttcgcag acaatcgtaa
    24361 acctattgga aaaacagccg aacccgatgc acagattaaa acaactgaga cagtaactca
    24421 tcaaatagat tctgaagcag tttcattgcc tgaatctgaa tcagcacaaa acggtatgcg
    24481 tgaactggag cagctaagac taagagaacc tacatctagt ccacgcccgg ttcaagtcat
    24541 ggatgactcc gaaggtttag atagccttgc aagcggggta aaaaatatgg ccgaactcga
    24601 agccatagtt tctgaacgta atttgaaaaa gcaatcagaa acagagtcga ccgaaatagt
    24661 cgaggattca gtcaaagcca aacatatgga ggccagagac cgtggaatgc agtttttacg
    24721 atggctggca gatggattag gagacggcag catagcagtc aatagaagtg gtgcaacagt
    24781 ccactttatt gaacagggga tgcttttggt tacgccagcc gtatttcgag attacgccgg
    24841 cggcgtgttc aataaatctg atacggaaag tctaggccct cttacacaaa aaggctttga
    24901 ggctttgagg ttgcatgcaa gaacgaaaaa aagctcattg catagggttc tgactacaaa
    24961 cggtagcaat cgcagactct tctattgcta ccttatccct gaacataata tcaaacatat
    25021 catacagcca ggttcaagac ctcagaataa taccgatatt aaattagatg aatccgactt
    25081 attagtaatg gaacaaaaat gagtgcccac ttccctgaaa acgtttatcg atcaccctat
    25141 gaatggatca gtgtagccgc atactccacc acagcggttt cagtagcaca tttgcccgta
    25201 cctccgacaa ttgcttatgg cagtttagct attttttcgt tggtttcagt caaacgtgcg
    25261 ttatcagcat tacgcattat gcgtttccgt aaaaatattc ggaagttgcc ctattatgcc
    25321 cttactgccg atgaaattcc ttcttctaat aaagctcttt ttcttgggaa aggttttaga
    25381 tggacacaga cacataccca acgtcttttt atggttcgcc agcctgagaa cgacggactt
    25441 cgtaacccct caaaactcta taaggcagcg cgaaaacatg agattgagac aatgggtaca
    25501 gactggattt cccgcataac tcggaaatcg gcatggtgga atccggttgc tcccttacct
    25561 ccggtcggag gttctcctga attacatggc attgaaattg aggaagatga tatttgggaa
    25621 gatttggggg agcgtgttgg ccacaaggtt gtattgggca caacccgcgt tggtaaaacc
    25681 cgattatgcg aaaccatgat tgttcaggat attcggcggg gggatgttac gatcgcattt
    25741 gatccaaagg gtgacgctga cctgatgact agaatgtatg tcgaatcgca aaaatcaaac
    25801 aggccgtttt atatgttcca tttgggtttc cccgagtaca gttgccggta caaccctatc
    25861 ggggattatg gccgtattac cgaggtggcg acaaggattg caaaccaact gccttctgaa
    25921 ggtcagtctg ctgctttccg agattttgta tggcgtttcg tcaacgtttt aacgaaaacc
    25981 atggaaggtt tatccattaa accaagctat gaaaatatct ataagtctgc tgcaaatgta
    26041 gatgaacttg caggggatta ctttaaaaag atattagata aacaccaccc tggttgggag
    26101 aaagacttcg acaattttga tatgcccgag tctgaagcaa aaagcgcagt caaaactggg
    26161 cgcaacttgg acacaatgaa gctcggttct ttcttgaagg ccaaaaagca taacgaacct
    26221 cttattgacg cattgggtgg tattttgtcc aatgacagat cctattttga gaagctagtc
    26281 agctccctgt atccattgct ggaaaaactg acgactggcg aagttgccaa gcttatcagc
    26341 ccggatacag aggatttgtc agatccaaga atcattatgg actggcgcaa agtcatggaa
    26401 tcgaatgccg tcgtctatat tggattggat tccctatctg atgccgaagt tgcagcagcc
    26461 gttggtaatg cgatgtttgc tgacttaacc tctcttgcgg gtcagatcta taaattcggc
    26521 catggggttg gacagagtgg aaaaacccag aaaagaaaag tctcaattca tgcagacgaa
    26581 ttcaatgaat taatcggtga cgaattcatt ccgttattga ataaggccgg tggtgccggc
    26641 taccaagtga cggtttatac acaaacctgg aaagacgtag aagccaaaat tggttcccct
    26701 gctaaagcag gtcaaattgg cggcaacttg aatacgatga ttatgcttag ggtgaaaact
    26761 gtcgaaacag cagagatgct gacgaatcag ttgccggaag tcaaaattat gaccagcact
    26821 ctagtctcaa gggctggtga tgttgccttc cctgatagtc acgaagattt tagcagtgga
    26881 aacgaagaca ggattggtgc tgagacagtt cctatgctga cacccgccga tttaacccaa
    26941 ttacccaaag gtcaagcatt cgcgcttatt gaaggtggcc agctatataa aattcgactc
    27001 cctctaccca aaaaggacaa gcaggaggaa atccctgcgc atattggaga aatggcggct
    27061 gatatgcgcg aagtttacag actaagccgg ccttcggctg aagaaaacgc atttacggag
    27121 ggaagtagtt atgccgtcta atacaagcgc atggtacatg aattcattgc ctttaaagtt
    27181 cttatttctc ccgtttaaag ctgcttatac cttattctta attgtgctgc ttatctttgg
    27241 tagttcggtg ttgacacaaa actattttgg taccggtaat ctcttggaag atgaaatcaa
    27301 atggtctgag tctgttggcc atgatgcgtt tccaggaaaa gaggttgacg atactataaa
    27361 tctgcaaatt gccagactta gttacaaggg gctgaaaacc gtattttttg acctgacatt
    27421 agtcaatgaa tcactgaaag ccaagtcaga agccgatttt ggttacaaat ttaaaaatta
    27481 tttagtcccc cgtatgaaat atctacagca attcgatact acgttgaagg ttatttcctt
    27541 acgtttggga aatttggcat taatcgtggg tatctcagta atctttgcaa tcccggtttt
    27601 gatagatggg ttagtgatga gagctatcag gcaggaaaat gcctcaaggg agagcgccgg
    27661 tatttatcac cgtgcaaaat attggagaac agggattatt tggttgggtt gcatgattta
    27721 tatgtgtgtg ccaatatcaa taccggccta cttgctctat ttacctctgg tagcagcaat
    27781 ttggatgatt ttcatgcaag ctaaatatct taagaaatat ctctaggctt ccagtggaat
    27841 ctaaatagtg cttttttagt tcattaaacg ggggcttaat gctttaactg acggtacaga
    27901 aatctcgaaa agcattttgc aaaatactta aaataagagt atagtaaaaa tgaaatattt
    27961 caagtttaac taatagttaa caatctagag taaacaatcg taatcgttaa atctgggtat
    28021 agattgcata atatacatta tgcgaactat cggaaacagt tgtacgtgta gccggtattg
    28081 gggaatacgt tgaccttcaa tacgattaca ggcagccgac acacaggata tgccgcccat
    28141 acttttttaa tccaaaacat tatatcaacg ggcgcaaaca gtaaatcgcc caacttatcc
    28201 caaaaattct cctgcttttt catcatttct tctgtcaaat ccttggaacg gtcgatttga
    28261 aaacacggcc ttacatacgt cccaattctc tttgcagtgc ttggatattt tgctgtctgt
    28321 ccaaaacatt gctttgcaat gcgttgattt tttgatggtt gatgttgccg ccttttgcca
    28381 gacgtgcttg tgataacatt ttttgggctt cagtcagggc tttgcgttca ttgcttaatt
    28441 ctgcttcgag aatggagcgt ctgctgttat ttacaggtgc ttgttgaggc ggcgtattgg
    28501 attttgccgg cttggatact gttttgaccg gggctttata tttgacagcc tgtccgccgt
    28561 ttgacggtga tggtgccggt tcgggagttt ggggcaggat atagcgttcg ctgctgtagt
    28621 tgccgattgg gggcaaatcg gttgagtggc agcttttaga cggcttggtg gtgtaaacgg
    28681 tttctccgtt gattgtgcag gtatagattt tggccgcatt cgcacccaat gggcttgaaa
    28741 tcagggaaag gttgattagg attaagggga gttttgattt cataatgtgt ttgatattcg
    28801 gataataatt tgattttttg aggatttttt gtaaggtttt cttggcgttt tatgggcttt
    28861 tcttttcggt tgtttatttt tgattttcct tcggggcttt ggcagcctgt ctgttctttt
    28921 tcccggcatg ttttttgttt ttctttttgt ttcctgtgtg tattttggct tcttgactaa
    28981 gttttttaat tttcaggcgg tatccgcctc cctgatggct gctgatttta ggtaaatcag
    29041 catcggcaca cgatcctgac gggtctgatg tatatacgct tttgccattt gagttgcaat
    29101 ggtatatgga ggcttgcgcc gccgcgccgg aaagggacag gagacccaag gcggcaaaaa
    29161 gtctgatgtt tataccggta aagtaaaaag gcggtggtaa cgtccgaatt ataccgttat
    29221 tggcaggcaa agataagcac cctgcccgcg cttctttatc gctcggcaaa ctgtttctcg
    29281 atttcagttt caatccgttc caattcttct tgacaagcca gccaagtttc ttcgatttgg
    29341 gcaagctgtg ttttgacttt tgccagctcg gataaggtat tttgcaattt ttctttgttt
    29401 gcttcaaggt aagcgtcttc ttgtgttaaa aatgcttcgc atcccgtctg aatttcagaa
    29461 agctgcacca tttctttttc ggctttgtcg attttctgtt gtatcggctt actgcgtcgg
    29521 gctttttctt gacggatttg cgcttcgata cgcttggtat ccttgcggtt ttggctttga
    29581 gtggaagcgg ctggcgcggt tgcggcgttt tcctgtgcca agcgccattg gcggtaatcg
    29641 ttcaaatcgc cgtcaaagtt tttcagacga cctttgtcta tcaagaggaa actgtcggtc
    29701 gtggcttcaa gcaggctgcg gtcgtgcgat acgacgatta atgcaccttg gaaactttgc
    29761 aacgcgagcg ttaaggcatg acgcatatcc aaatccagat ggttggtcgg ttcgtcaagc
    29821 agcagcaggt tcggcttttg ccagataatc atggcaaggg caaggcgggc tttttctccg
    29881 ccggaaaaag gttcggtttt ctgtaacgcc atatcgccga caaaatcgaa gccgccgagg
    29941 aaatttcgga tttcttgttc gcgtacttcg ggagaaagct gctgtatatg ccaaacaggg
    30001 ctttggtcgg cgcggatggt gtcaagctgg tgttgggcga aatagccgat attaagtttt
    30061 tcggaatgga cgatgctgcc ggagagtaaa tcgatttttc ctgccaaagc tttgataaac
    30121 gtagatttac cgctgccgtt gacacccaat agcccgtagc gtgcgccgct ttccagcgac
    30181 agggtaatgt catgcaggac ggttttacct tcgtaaccca aatcagcgtg ctccagcttc
    30241 agcaaaggat tgggcagatg gtcgggattg taaaactcaa aggaaaactc gctgtccaga
    30301 tgtgcgggag cgatgcgttc gagcttcgcc aacgccttca tgcggctttg agcttgaacg
    30361 gctttagtgg ctttggcttt gaagcggtcg ataaaggatt gcagatgttt gatttgcgcc
    30421 tgctgtttga catatgcggc ttgttgttgc gccagacgct gcgcgcgttc ggtttggtaa
    30481 aaatcgtaat tgccgctgta ttgcgtgagc ttttgctgcg acaattcaat ggtttgggtg
    30541 gtggccgcat tgaggaaatc acggtcgtgg gaaatgatga tttgcgtgca gggtaaagag
    30601 gcaaggtggt tttccaacca caagacagtt tccaaatcca aatggttggt cggttcgtca
    30661 agcaagagca aatcagcgcg gcaaatcagg gcttgcgcaa gattcaggcg catacgccag
    30721 ccgccggaaa aggatttgac gggacggctg tgttcttctt gcgaaaaacc cagcccgttc
    30781 aacaattttg ccgcgcgggc cggcgcggta taggcgtcga tttcttccaa tttggcatga
    30841 tattccgcct gcttcatgcc gtcattttgc acttccgcct gcctcaatgc cgtctgaaaa
    30901 gcctgcaact cggcatcgcc ctgcaaaacg taatccaaag cggaaatatc caaatcgggc
    30961 gtttcttggg aaacggaagc gagccgccag tttttcggaa tcgagatatc gccgccgtcc
    31021 tgagtgattt cgcccttgat taaggcaaac aggctggatt tgcccgtccc gttcttaccg
    31081 atcaaaccga cgcgctgacc gggattgacg gcagcggaag ccttgtcgag caggactttc
    31141 aaaccgcgtt gcagggtgag gtttttgatt tcaatcataa cggaaacatc gtcgggcggg
    31201 aaaagcccgt attttacctg aaagtcagtg ccgatgccgt ctgaaacggg aaatttacgg
    31261 ctgaagccaa gcccaagccc tgcgcccttc cgagtgcagg aaaaccaatg tcctgaatgc
    31321 cgaatcggta ttcatgcatt ccacgctgat tccgattcgg gaaaaatccg ccatgatttt
    31381 gggatggata aactcctgag ccgcgcccgt cccgataatc aatatttccg gatagtcaac
    31441 aggtttgatg tcggacaaca ggttttccgg agtcaggtcg gacaaggttc ggcattgcgg
    31501 caggcagacc gaatccttat gtacaagcac gggtttatgg aaactttgcc ccgccagccg
    31561 gattccgccc gcgccgcatt catattctgc aaactgtccg tctatcggat tttcttcaaa
    31621 caacattttt taccccgttg ctgcatcatc tgcaccgaaa ggggtgcaaa atcaggcgaa
    31681 ttaatgtagg attggcagat ttcatctgac gcgcctgccg atttcagacg gcatttgatt
    31741 caaagtgcgg cacaattata ccggcagcgg atattttcgt ctttcaatat ttacatttca
    31801 gtcggcttac aaggagacac aatgaagcca gtaaacatcg gtcttttagg cttaggtacg
    31861 gtcggcggcg gtgcggctgc cgtgttgcgg gacaacgcag aggaaatttc ccgccgcttg
    31921 gggcgcgaaa tccgtatttc tgccatgtgc gacttaagcg aagaaaaagc ccgccaaatc
    31981 tgcccgtccg ccgcctttgt caaagatccg ttcgaactgg tcgcgcgtaa agacgtcgat
    32041 gtcgtcgtcg aattgttcgg cggcacgggc attgccaaag aagcggtgtt gaaagccatt
    32101 gaaaacggca aacacatcgt taccgccaac aaaaaactgc tcgccgaata cggcaacgaa
    32161 atcttcccgc tggcggaaaa acaaaacgtc atcgtccaat ttgaagcggc agtagcgggc
    32221 ggtatcccaa tcatcaaagc cctgcgcgaa ggtttggcgg caaacaggat taaatccatc
    32281 gccggcatta ttaacggcac cagcaacttc atcctctccg aaatgcgcga aaaaggcagc
    32341 gcgtttgccg acgtattgaa agaagcgcag gcattgggtt atgccgaagc cgacccgacc
    32401 ttcgacatcg aaggcaacga cgcaggccat aaaatcacca tcatgagcgc gctggcattc
    32461 ggtacgccga tgaacttctc cgcctgctat ctcgaaggca tcagcaaact cgacagccgc
    32521 gacatcaaat acgccgaaga actcggctac cgcatcaaac tgttgggcat cacccgcaaa
    32581 accggcaaag gcatcgaatt gcgcgtccat ccgaccctga ttcccgaaag ccgcctcttg
    32641 gcaaacgtcg acggcgtgat gaacgccgtg cgcgtcaacg ccaatatggt cggcgaaacc
    32701 ttatattacg gcgcgggcgc gggcgcattg ccgaccgctt ccgccgtggt tgccgatatc
    32761 atcgacatcg cccgcctggt tgaagccgac accgcccacc gcgtaccgca tctggcgttc
    32821 caacccgccc aagtccaagc gcaaaccatc ctgcctatgg acgaaattac cagcagctac
    32881 tacctgcgcg tccaagccaa agacgaaccg ggcacgctgg ggcaaatcgc cgcgctgttg
    32941 gcacaagaaa acgtgtccat cgaagcctta atccaaaaag gcgttatcga tcagaccact
    33001 gccgaaatcg tgattctgac ccacagcacg gtcgaaaaac acatcaagtc ggcaatcgca
    33061 gccatcgaag cactggattg tgtggaaaaa ccgattacca tgatccgcat ggaaagcctg
    33121 catgactgag ccgaaacacg aaacgccgac ggaagagcag gttgccgcgc gcaaaaaagc
    33181 aaaagccaaa atccgcacca tccgcatttg ggcgtgggtc attttggcgt tgctcgcttc
    33241 aaccgccctg ctctcccaat gcgcgatgtc caaaccgcag gcaaaacaga aaattgtcga
    33301 gtcttgcatg aaaaatattc cgtttgctga aaaatggcag aacgatttga aagcgcgcgg
    33361 cttggatgcg gacaataccc gtctcgccgt cgactactgc aaatgtatgt gggagcagcc
    33421 tttggacgga ttgagcgaga aacagatcag ctccttcggc aaactcggtg cacaagaaca
    33481 gcttgacctg ctcggcggcg caaacgcgtt tgaaactcga gacaaacaat gtgtcgcgga
    33541 tttgaaagcc gattgaacct caggtctgga atgccgtctg aacgcgggga aaagcgtttc
    33601 agatggcatt tttccatccg ggtttgacgg gcagcccttc atccgaatcc gacgggcaaa
    33661 cccctatcct gattcggcgg ggaaaacaaa aagccgattc ttcaaggaat cggcttttgc
    33721 tgaaaaacag tttacagtgc gtctttcaat gctttacctg cacggaattt aagcgttttg
    33781 gcggcggcaa tggtcagagg ctcgccggtt ttggggttgc ggccttggcg ttccgcacgt
    33841 tcgccgacgt agaaagtacc gaaaccgacc agagtaacgg tgtcgccttg tttcagggcg
    33901 ttggttactg cgttggtagt ggcatccaaa gctttttgtg cggcggcttt ggaaatatcg
    33961 gcttcttgag caatcgcttc gatcaattca gacttgttca caatcagtcc cttcctgtct
    34021 taaaaaatga tgaaatgccc gaatactcgg ggtttgtact gcttgagcaa ctttcgcttt
    34081 atagcaattc tgaaattgcc gtgtcaagca aaaaatacgg aatcgcccta ttttacaggc
    34141 tttcaggacg aaaccgcatt tttacaacac atttcctgcg tttcaatgtt tgattgccct
    34201 gctgcggggt ttcggttttg aagcggattc cgccgccgct tccgcggcag aaggttctgc
    34261 ccaagactca ggccggcttt ccaaacccaa agccaatacc tcgtctatcc atttgaccgg
    34321 atggatggtt aatccggttt tcacgttttc agggatttct tccaggtctt tgacattgtc
    34381 tttcggaatc aggacgtgtt tgatgccgcc gcgaagcgca gccaacagtt tttccttcag
    34441 accgccgatc ggcaggactt cgccgcgcag ggtaatttcg cccgtcatgg caacatcggc
    34501 acgcaccgga attttggtaa aggcggacac catcgccaaa gtcatagcaa tgcctgcgct
    34561 cggaccgtct ttcggcgttg caccttcggg aacatggaca tgaatgtctt ttttctcgta
    34621 aaaatcagga gccaaaccta ctgattccgc acgggaacgg acaacagacc atgcagcgga
    34681 tacagattcc ttcatcacat cgcccaactg gccggtacac tggatcatgc ctttacccgg
    34741 taatgctgcg gcttcgacag tcagcaattc gccgccgact tccgtccaag ccaaaccggt
    34801 aacctgtccg atgcggtttt cactttcggc aacgccgtaa tcgaagcggc gcacgcccaa
    34861 gtagtcatgt aggtttttct cattgacttt aaccgcccta ggcttggctt tgctggtttt
    34921 cttggtttca gacaacctct tcttatcttc gttcaaggta atctgcatca ccaccttacg
    34981 gcagattttg gcaatttcgc ggtcgagcga acgcacgccc gcctctcggg tgtaataacg
    35041 gataatatcg cgtaccgcgc tttcttcgac caccaattcg ccttctttca cgccgttgcg
    35101 tttcatttgc ttcggtacga gatactgcat cgcgatattg attttctcgt cttcggtata
    35161 gccggacaga cggatgattt ccatacggtc aagcagcggg gtcgggatat tcaggctgtt
    35221 tgaagtcgca ataaacatca cgtcgctcaa atcgtaatcc acttcggcat aatgatcggc
    35281 gaacttgttg ttttgttcgt gatcgagcac ttcaagcaac gcgctggcgg gatcgccccg
    35341 aaagtcgctg cccaatttgt cgatttcgtc gagcaggaac aacgggtttc ttacgcctgc
    35401 ttttgccata ttctgcaaaa tcttgccggg catagaaccg atataggtac ggcggtgccc
    35461 gcggatttcg ctttcgtcgc gcacgccgcc caaggccata cggacatatt tccgccccgt
    35521 tgctttggcg atggattcgc ccaaagaggt tttgcccacg cccggagggc cgaccaaaca
    35581 cagaatcgga cctttgagct tgtccatacg tttttggacg gcgaggtatt ccaaaatccg
    35641 ttctttgact ttttccaggc cgtagtggtc ggcatccagc accagtccgg ctttggcgat
    35701 gtctttgctg acgcgggatt ttttcttcca cggcagtccg agcagggtgt cgatgtagtt
    35761 gcgtacgacg gtggattcgg cagacatcgg cggcatcatt ttgagttttt tcagttcgga
    35821 caggcatttt tcttccgctt ctttggtcat acccgccttt ttgatgcctg cctccaaggc
    35881 atccagttcg ccgttttcgt cttcttcgcc caattctttg tgtatcgctt taatctgttc
    35941 gttcagataa tattcgcgtt gggatttttc catttggcgt ttgacgcgtc cgcgtatgcg
    36001 tttttcggcc tgcataatgt cgagttcgga ttccaacttt gccagcagga attccatccg
    36061 tttgccgatt tcgggaatct ccaaaatctg ttggcgttgc gccagtttca actgcaaatg
    36121 cgctgcgacc gtatcggtta gccggctgtt ttcggcaatg ccgttgatgc tgccgataat
    36181 ttcggcaggg atttttttat tgagtttggc gtattgttca aactgcgcca acagggtgcg
    36241 gcgcacggct tcgaggtcgg tattgccgcc cgtgtcttct tccacgaccg cctctatatg
    36301 agaaacgaac agcccgcccg tgtcttcgat ggtcaaaaca cgtccgcgat acagcccttc
    36361 gaccagcact tttaccgtgc cgtcgggcag tttcaacact tgcaggactt gtgcgacggt
    36421 accggtctga tacaggtcgg cggcaaccgg ttcttctacc gccgcatcgg tttgcgccaa
    36481 caggaaaacc ggttcctcgc gggtaatggc gttttccagt gcggcgatgg atttcggcct
    36541 gccgacgaac agcggcagaa ccatatgcgg gtaaacgacg acatcccgta aaggaagggt
    36601 cgccaaagca gcatattcct caaaatgctt ttctttttgt gtcatggata ctctcttgtg
    36661 tctgacagga tacggattgc cgcgtaaatt ggggttgaaa gtattatttc aagcatacgc
    36721 ggtttattta tggagtttga tgccatgccg tctgaaacat tccggcttca gacggcatgg
    36781 gcttggaaag acaaggcggg aacaaaaaaa cagttctgtg ttgccgctcc ttgctgtacc
    36841 atccgtatgg tttgcggttc tgccgcccta ttttcaaaac atgacgcagg tatcccatgt
    36901 cttcttttat ttttcccgat aacgggttcg aatggcgcaa cgaaagtctg caaaagccgc
    36961 ccaaaggttg gtattatgtc cgcgaaagcg gtgcagacgg cattttgaag gctacctatc
    37021 aaaatattgc aactgtctgg cagggcgatt tccacaatgc caaacaggtg ctttcggcaa
    37081 tgaagaagaa ggtccgcaaa cctgccgccg tccgttccga tgcggatatc gccgcccttt
    37141 tccacgccca ccgtatgaag caggcgcaac agagccgtat tctgaatatg cttgccgttg
    37201 aaatccgccc cggtttcgtg ttggacaaca aacgcgcgcc cgatatacgc gccgctttgc
    37261 tcgacgtgta cggagaggcg gacggcaaac cgttttttct gccgctcaat ctgctgctgg
    37321 ggtttatggg tgtgcatgag tggcataaga aagatactac tttaattgac aacagcat
//
LOCUS       DAXDSD010000005        37026 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXDSD010000005 DAXDSD010000000
VERSION     DAXDSD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37026
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.005"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            218..838
                     /locus_tag="ACFB2Q_000169"
     CDS             218..838
                     /locus_tag="ACFB2Q_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC0917293.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSKGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACLNLLIAASVWLRYRKDGYGEH"
     gene            861..2312
                     /locus_tag="ACFB2Q_000170"
     CDS             861..2312
                     /locus_tag="ACFB2Q_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HFC0917294.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIAGRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDIFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNSTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIRMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            2773..3129
                     /locus_tag="ACFB2Q_000171"
     CDS             2773..3129
                     /locus_tag="ACFB2Q_000171"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC0917295.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            3120..3602
                     /locus_tag="ACFB2Q_000172"
     CDS             3120..3602
                     /locus_tag="ACFB2Q_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC0917296.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            3615..4208
                     /locus_tag="ACFB2Q_000173"
     CDS             3615..4208
                     /locus_tag="ACFB2Q_000173"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC0917297.1"
                     /translation="MASIQNLYETVVGVLGDQAGKVISALGEITVECLPEHYISVMTA
                     LHDHEDLHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVADIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            4198..5454
                     /gene="nuoD"
                     /locus_tag="ACFB2Q_000174"
     CDS             4198..5454
                     /gene="nuoD"
                     /locus_tag="ACFB2Q_000174"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC0917298.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            5454..5927
                     /gene="nuoE"
                     /locus_tag="ACFB2Q_000175"
     CDS             5454..5927
                     /gene="nuoE"
                     /locus_tag="ACFB2Q_000175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC0917299.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            6317..7618
                     /gene="nuoF"
                     /locus_tag="ACFB2Q_000176"
     CDS             6317..7618
                     /gene="nuoF"
                     /locus_tag="ACFB2Q_000176"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC0917300.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            7618..7917
                     /locus_tag="ACFB2Q_000177"
     CDS             7618..7917
                     /locus_tag="ACFB2Q_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917301.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERYGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFILARQGHGVVVAMQ"
     gene            8331..10593
                     /gene="nuoG"
                     /locus_tag="ACFB2Q_000178"
                     /pseudo
     CDS             8331..10593
                     /gene="nuoG"
                     /locus_tag="ACFB2Q_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
     gene            10596..11672
                     /gene="nuoH"
                     /locus_tag="ACFB2Q_000179"
     CDS             10596..11672
                     /gene="nuoH"
                     /locus_tag="ACFB2Q_000179"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC0917302.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPRGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            11753..12232
                     /gene="nuoI"
                     /locus_tag="ACFB2Q_000180"
     CDS             11753..12232
                     /gene="nuoI"
                     /locus_tag="ACFB2Q_000180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC0917303.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            12244..12915
                     /locus_tag="ACFB2Q_000181"
     CDS             12244..12915
                     /locus_tag="ACFB2Q_000181"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC0917304.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            12912..13217
                     /gene="nuoK"
                     /locus_tag="ACFB2Q_000182"
     CDS             12912..13217
                     /gene="nuoK"
                     /locus_tag="ACFB2Q_000182"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC0917305.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            13220..15244
                     /gene="nuoL"
                     /locus_tag="ACFB2Q_000183"
     CDS             13220..15244
                     /gene="nuoL"
                     /locus_tag="ACFB2Q_000183"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC0917306.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRTKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPGATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAVKYSTLPGSGVAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLATAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGNFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            15387..16385
                     /locus_tag="ACFB2Q_000184"
     CDS             15387..16385
                     /locus_tag="ACFB2Q_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC0917307.1"
                     /translation="MDKRLFDGTVRFEHVTGVGTMQVDFQPNQRVYTMIGENGVGKTK
                     FLECLFTVLLFTYRMENINIGFSWIDLQRLPLKIVELPNGKNEDFRRFSERSNQGFNI
                     NEDVLFSGYQHRLPFVYLPAQSRGWVKKTNFQTQSLGSSQGRASKYLGNLFSVFQSGH
                     SSFSLKSLNMENDLEEWIIQRARSANPYQANEDNRKIEIDTLLFLLNQFDGRIAPDFL
                     EIKGDDRIFIKLDNQKRELSGLSSGFTSILKIMQSIVAGYSYFTNEQQIANVRGVVLI
                     DEIESHLHNEWQIKIVPLLKKLFPNTTFFITTHSSLVISQLEQGGPIVWYVARRTV"
     gene            16358..16555
                     /locus_tag="ACFB2Q_000185"
     CDS             16358..16555
                     /locus_tag="ACFB2Q_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C nitrite reductase"
                     /protein_id="HFC0917308.1"
                     /translation="MVRSKEDGVVYGKMIDYPSNASFVDLLNEAFGVDLNKKKIERAQ
                     ERNQEQAKKPYWNWYVRNWAD"
     gene            16566..16790
                     /locus_tag="ACFB2Q_000186"
     CDS             16566..16790
                     /locus_tag="ACFB2Q_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFC0917309.1"
                     /translation="MTVYLLDTNYLVYLADDDSDEEKRKAVLSDMAEKLQQDDNRFVI
                     TPLIRYEVLRGVDWGKSEKLSRLTGVLAQF"
     gene            16821..17027
                     /locus_tag="ACFB2Q_000187"
     CDS             16821..17027
                     /locus_tag="ACFB2Q_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917310.1"
                     /translation="MASNLYRFDKFEAERDNTPKNLEKRKFDMFHYATASVNNLEILS
                     HDTDVNKIKDLHERMRLEDSAELA"
     gene            17137..18633
                     /locus_tag="ACFB2Q_000188"
     CDS             17137..18633
                     /locus_tag="ACFB2Q_000188"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC0917311.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            18643..20088
                     /gene="nuoN"
                     /locus_tag="ACFB2Q_000189"
     CDS             18643..20088
                     /gene="nuoN"
                     /locus_tag="ACFB2Q_000189"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC0917312.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHTGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            20200..20499
                     /locus_tag="ACFB2Q_000190"
     CDS             20200..20499
                     /locus_tag="ACFB2Q_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC0917313.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(20616..21512)
                     /locus_tag="ACFB2Q_000191"
     CDS             complement(20616..21512)
                     /locus_tag="ACFB2Q_000191"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC0917314.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(21496..21720)
                     /locus_tag="ACFB2Q_000192"
     CDS             complement(21496..21720)
                     /locus_tag="ACFB2Q_000192"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC0917315.1"
                     /translation="MKKNTPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGTKELNLESDE"
     gene            complement(21833..22756)
                     /gene="rsgA"
                     /locus_tag="ACFB2Q_000193"
     CDS             complement(21833..22756)
                     /gene="rsgA"
                     /locus_tag="ACFB2Q_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC0917316.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            complement(22761..24617)
                     /locus_tag="ACFB2Q_000194"
     CDS             complement(22761..24617)
                     /locus_tag="ACFB2Q_000194"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC0917317.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMAFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSAAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRSGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            complement(24643..24780)
                     /locus_tag="ACFB2Q_000195"
     CDS             complement(24643..24780)
                     /locus_tag="ACFB2Q_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917318.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSLLSDGIVFDA
                     V"
     gene            complement(24780..25364)
                     /gene="ruvA"
                     /locus_tag="ACFB2Q_000196"
     CDS             complement(24780..25364)
                     /gene="ruvA"
                     /locus_tag="ACFB2Q_000196"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC0917319.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(25446..25772)
                     /locus_tag="ACFB2Q_000197"
     CDS             complement(25446..25772)
                     /locus_tag="ACFB2Q_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC0917320.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQ
                     DKIISRAQAEKAAWARVGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(25833..26519)
                     /locus_tag="ACFB2Q_000198"
     CDS             complement(25833..26519)
                     /locus_tag="ACFB2Q_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC0917321.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            complement(26647..27111)
                     /gene="trmL"
                     /locus_tag="ACFB2Q_000199"
     CDS             complement(26647..27111)
                     /gene="trmL"
                     /locus_tag="ACFB2Q_000199"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC0917322.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            complement(27176..27901)
                     /locus_tag="ACFB2Q_000200"
     CDS             complement(27176..27901)
                     /locus_tag="ACFB2Q_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC0917323.1"
                     /translation="MNFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCNTDLASFR
                     TDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIRALKHLADLGM
                     AQPLADLTMQNPPDRLSDECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            27900..28649
                     /gene="bioH"
                     /locus_tag="ACFB2Q_000201"
     CDS             27900..28649
                     /gene="bioH"
                     /locus_tag="ACFB2Q_000201"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC0917324.1"
                     /translation="MPDAAKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADGIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTAAEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLAR
                     CGTPQALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRL
                     VVMEKAAHAPFLSHAEVFAALYRDFVEGGLT"
     gene            28646..29434
                     /locus_tag="ACFB2Q_000202"
     CDS             28646..29434
                     /locus_tag="ACFB2Q_000202"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC0917325.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPGHILLAGADADISRS
                     LLAKRYPLAVFEEYDSRADFLAAAAAARKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCAGAIFEREGGLGITLETVYGHAVKKLMLPQGENVVRFFPKR"
     gene            complement(29515..29622)
                     /locus_tag="ACFB2Q_000203"
                     /pseudo
     CDS             complement(29515..29622)
                     /locus_tag="ACFB2Q_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            29744..31117
                     /locus_tag="ACFB2Q_000204"
     CDS             29744..31117
                     /locus_tag="ACFB2Q_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC0917326.1"
                     /translation="MDWRGNKPLGAAELADLKPLYKDFMYWERGLHMYKASAVVPTGY
                     VRVGNTAPLCGEDTQRYASFWGDGYDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAG
                     REYGISKQNLSDVWDDFEDAMELKAFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLEN
                     HGQTVLDFSNTRITDLSVDMTGVESLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLIL
                     EVGKSVPKVRGLENLTAVNVMGIADFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSG
                     FEDLEVFTAVDLFGFGADDIPHPDRLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLD
                     LWIEKARKPEWLAQNFDNPFRDWDGAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPEN
                     TGEGLAEAVKAYTGGFNKMDKKHFIDTVEREDIAEALETILDLIPDGSCADKEKLFEI
                     FDKNRNF"
     gene            complement(31154..33457)
                     /gene="recQ"
                     /locus_tag="ACFB2Q_000205"
     CDS             complement(31154..33457)
                     /gene="recQ"
                     /locus_tag="ACFB2Q_000205"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC0917327.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKILKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(33520..34302)
                     /gene="trpC"
                     /locus_tag="ACFB2Q_000206"
     CDS             complement(33520..34302)
                     /gene="trpC"
                     /locus_tag="ACFB2Q_000206"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC0917328.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLRGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            complement(34353..35312)
                     /locus_tag="ACFB2Q_000207"
     CDS             complement(34353..35312)
                     /locus_tag="ACFB2Q_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFC0917329.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRRELLPDSTGKT
                     KTAYDFSAGGISPDAPLVVLFHGLEGSSRSHYAAELMLAVRNRGWHGAVVHFRSCGGV
                     ANTAPVFYHLGDTAEIAFALDTLAARYREIYAVGVSLGGNAPAKYLGEQGKKALPHAS
                     AAVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFD
                     DRFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEASEA
                     VTLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            complement(35445..36983)
                     /gene="murJ"
                     /locus_tag="ACFB2Q_000208"
     CDS             complement(35445..36983)
                     /gene="murJ"
                     /locus_tag="ACFB2Q_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC0917330.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
BASE COUNT         8035 a         9489 c        10470 g         9032 t
ORIGIN      
        1 aaaatcgagt ttatcctagt tgtccaggac agccccttaa tttttataac gtcatgaagc
       61 cgcacagcag tttgaacagt gatacgccgt ttgcgggctt acgagtttat tttcccggcc
      121 tgcagtttga gcaatgcggt gatttcctac ggctaataca aatgtttaca cattaataca
      181 tttcatttat agttccgcct atttgaaaat agaaaatatg aattcgaccg caagtaaaac
      241 cctgaaagga ttgtcgctgg tgtttttcgc ctctggcttc tgcgccctga tttaccaggt
      301 cagctggcag aggcttctat tcagccacat aggtatcgat ttgagttcga ttactgtcat
      361 tatttctgta tttatggtcg gcttgggtgt aggtgcgtat ttcggcggac gcattgctga
      421 ccgttttcct tcaagtatca tccccctgtt ttgcatcgct gaagtatcca tcggtctgtt
      481 cggtttggta agcaagggtc tgatttccgg cttggggcat cttttagttg aggctgattt
      541 gcccatcatc gctgctgcca atttcctctt attgctgctt cctaccttta tgatgggcgc
      601 gaccttgccc ttgctgacct gtttttttaa ccggaaaata cataatgttg gcgagtctat
      661 cggtacctta tattttttca acactttggg tgcggcactc ggatcgcttg ccgccgccga
      721 atttttctac gtctttttta ccctctccca aaccattgcg ctgacagcct gccttaacct
      781 tctgattgct gcttcagtat ggctgcgtta cagaaaggat ggatatggtg aacactaaac
      841 cgaatactag tttgatttat atgctttctt tccttagcgg attattgagc ttgggtatag
      901 aagtcttgtg ggtaaggatg ttttcgttcg cagcacagtc cgtgcctcag gcattttcat
      961 ttactcttgc ctgttttctg accggtatcg ccgtcggcgc gtattttggc aaacggattt
     1021 gccgcagccg ctttgttgat attcccttta tcgggcagtg cttcttgtgg gcgggtattg
     1081 ccgatttttt gattttgggt gctgcgtggt tgttgacggg tttttccggc ttcgtccacc
     1141 acgccggtat cttcattacc ctgtctgccg tcgtcagggg gttgattttc ccacttgtac
     1201 accatgtggg tacggatggc aacaaatccg gacgacaggt ttccaatgtt tatttcgcca
     1261 acgttgccgg cagtgcattg ggtccggtcc ttatcggctt tgtgatactt gatttcttgt
     1321 ccacccaaca gatttacctg ctcatctgtt tgatttctgc tgctgtccct ttgttttgta
     1381 cactgttcca aaaaagtctc cgactgaatg cagtgtcggt agcagtttcc ctaatgttcg
     1441 gcatcctcat gttcctactg ccggattctg tctttcaaaa tattgctggc cgtccggata
     1501 ggttgattga aaacaaacac ggcattgttg cggtttacca tagagatggt gataaggttg
     1561 tttatggggc gaatgtatac gacggcgcat acaataccga tatattcaat agtgtcaacg
     1621 gcatcgaacg tgcctatctg ctaccctccc tgaagtccgg catacgccgc attttcgtcg
     1681 ttggattgag tacaggttcg tgggcgcgcg tcttgtctgc cattccggaa atgcagtcga
     1741 tgatcgttgc ggaaatcaat ccggcatacc gtagccttat cgcggacgag ccgcaaatcg
     1801 caccgctttt gcaggacaaa cgtgttgaaa ttgtattgga tgacggtagg aaatggctgc
     1861 gtcgccatcc tgatgaaaaa ttcgacctga ttttgatgaa ttcgacttgg tactggcgtg
     1921 cctattccac taacctgttg agtgcggaat ttttaaaaca ggtgcaaagc caccttaccc
     1981 cggatggtat tgtaatgttt aataccacgc acagcccgca tgcttttgct accgccgtac
     2041 acagtattcc ctatgcatac cgctacgggc atatggtagt cggctcggca accccggtag
     2101 ttttccctaa taaagaactg ctcaagcaac gcctttcccg gttgatttgg ccggaaagcg
     2161 gcaggcacgt atttgacagc agcaccgtgg atgctgcagc acaaaaggtt gtctctcgta
     2221 tgctgattcg gatgacggaa ccttcggctg gggcggaagt cattactgac gataatatga
     2281 ttgtagaata caaatacggc agagggattt aaccgtctta aagggtttca ggcaacgcag
     2341 gttttaggta acgtcctgct agttcaaaaa aaccgcatca cagcagtcgg gacaaaatgg
     2401 tttaaacatt ttgtcccgaa ttcttattcc tatatatagc ggattaacaa aaatcaggac
     2461 aaggcggcgg gccgcaggca gtacaaatag tacggaaccg gttcgcctgg tgcttgggca
     2521 ccttagggaa tcgttcccct tgagctaagg cggggcaacg ccgtaccggt ttttgttaat
     2581 ccgctatacc acgaattacg gtgtaaaaat ttatatgacc ttataaaatc aaataagaat
     2641 cgttatcata acatgattgt atttattggg tttttttggg cgttttgccg atatttacct
     2701 tttaatggtt tttgaaattc gctaaaatac gaaattattg tagaaatttt gttaacggat
     2761 ttgggtgtaa ccatgttgtc cgcttatttt cccgtctttg tctttatcct cgtcggcctc
     2821 gcggccggcg tactgtttat cctgctcggc acgattttgg gcccgaaacg ccactatgcc
     2881 gaaaaagacg cgccttacga atgcggtttt gaagcctttg aaaacgcaag gatgaagttc
     2941 gacgtgcgct attacctcgt cgccatcctg ttcatcctct tcgatttgga ggttgcgttt
     3001 atgctgccgt gggcagtcgt gttcaaagat ttgggcgcat acggcttttg gtctatgctg
     3061 gtgtttatcg tcgtcctgac ggtaggcttt gtttacgaat ggaaaaaagg tgcgctggaa
     3121 tgggaataga aggcgttttg aaaaaaggtt tcatcaccac cagcgcggat acggtgttga
     3181 actatatgcg taccggttca ttgtggccgg ttactttcgg attggcctgc tgcgccgtgg
     3241 aaatgatgca tgcgggtatg gcgcgttatg accttgaccg tttcggtatc attttccgtc
     3301 cgtccccacg tcaggccgac ctgatgattg tggcaggtac gctgaccaat aaaatggcgc
     3361 cagccctgcg tcgcgtgtac gaccagctcg ccgagccgcg ctgggtattg tctatgggtt
     3421 cgtgtgccaa cggcggcggc tattatcact attcttattc cgttgtgcgc ggtgccgacc
     3481 gtgtcgtgcc ggtggacgtt tatgtgccgg gttgtccgcc gactgcggaa gctctgattt
     3541 acggcctgat tcagctccaa caaaaaatca agcgcacttc caccatcgcg cgtgacgagt
     3601 aaggagagga cgatatggca agcattcaaa acttatacga gaccgtcgtc ggcgtgcttg
     3661 gcgatcaggc aggcaaagtc atttcagctt tgggcgagat taccgtcgag tgtctgcccg
     3721 agcactatat ttcagtcatg accgcattgc acgaccatga agatttgcat ttcgagcttc
     3781 tggttgactt gtgcggcgtc gattacagca cttacaaaaa cgaagcatgg cagggcaaac
     3841 gctttgccgt tgtcagtcag ttgctttccg ttaaaaacaa tcaacgcatc cgcgtacgcg
     3901 tctgggtttc agacgacgac ttccctgtag tcgaatccgt agccgatatt tacaacagcg
     3961 cggattggta cgagcgcgaa gccttcgatt tgtacggcat catgttcaac aaccatcccg
     4021 acttgcgccg catcctgacc gattacggct ttgtcggaca tccgttccgc aaagacttcc
     4081 cgatttccgg ctatgtggaa atgcgttatg acgaagagca aaaacgcgtg atttaccaac
     4141 ctgttaccat tgagccgcgc gaaatcacac cgcgtatcgt ccgtgaggag aactacggtg
     4201 gccaataaat taagaaacta caccatcaac ttcggcccgc aacaccctgc ggcgcatggc
     4261 gtattgcgta tgattttgga gctggacggc gaacaaatcg tccgtgccga tccgcatatc
     4321 ggcctcctgc accggggcac cgaaaaactg gcggaaacca aaacctatct gcaagccctg
     4381 ccctatatgg accgtttgga ctacgtttcc atgatggtca acgagcaggc gtattgcttg
     4441 gcggtagaaa aacttgccgg tatcgatgtg cctatccgcg cccaatacat ccgcgtgatg
     4501 ttcgccgaag taacgcgcat cctcaatcac ttgatgggca tcggttcgca cgccttcgac
     4561 atcggcgcga tgaccgccat cctctacgcc ttccgcgacc gcgaagagct gatggacttg
     4621 tacgaagccg tgtccggcgc acgtatgcac gccgcctact tccgtcccgg cggcgtttac
     4681 cgcgacctac ccggctttat gcccaaatac gagagcagca aattccgcaa tgccaaagta
     4741 ttgaagcagc tcaacgaatc ccgcgaaggc acgatgcttg actttatcga tgccttctgc
     4801 gaacgcttcc ccaaaaatat cgacacactc gaaaccctcc tgaccgacaa ccgcatctgg
     4861 aaacagcgta ccgtcggcat cggcgttgtc tctcccgagc gcgccatgca aaaaggcttt
     4921 accggtgtga tgcttcgcgg ttccggcgtg gaatgggacg tgcgtaagac acagccctac
     4981 gaagtgtacg acaaaatgga tttcgacatc cccgtcggcg tcaacggaga ctgctacgac
     5041 cgttacctct gccgtatgga agaaatgcgc caatccgtac gcatcatcaa acaatgcgcc
     5101 gactggttgc gcgtcaatcc cggcccggtc attaccgcaa accacaaatt cgctccgccc
     5161 aaacgtaccg aaatgaaaac aggtatggaa gacctgattc accatttcaa actctttacc
     5221 gagggtatgc acgttcccga gggcgagacc tacaccgctg tcgaacaccc gaaaggcgag
     5281 ttcggcgttt acatcatttc agacggcgca aacaaaccct accgcctgaa aatccgcgca
     5341 cccggcttcg cccacctgca aggcatggac gaaatggcaa aaggccatat gctcgccgac
     5401 gtcgttgcca tcatcggtac gcaggacatc gtattcgggg aggttgaccg ataatgttat
     5461 ccgcaaaatc cttaaaacaa atcgacattg agttggcgaa atatcccgcc gaccaacgcc
     5521 gttccgccat catgggcgcg ttgcgtatcg cccaaaccga aaaaggctgg ctcgcccccg
     5581 aaaccatcgc ttttgttgcc gactacatcg gcatcacgcc ggcgcaggca tatgaagtcg
     5641 ccactttcta caatatgtac gaccttgagc ctgtcggcaa atacaaactg accgtttgta
     5701 ccaacctgcc ctgcgctctg cgcggcggta tggcgaccgg cgaatacctc aaacaaaaac
     5761 tcggtatcgg ctacggcgaa accacgcccg acggcaaatt tacccttgtc gaaggcgaat
     5821 gcatgggcgc gtgcggcgat gcccccgtta tgctggtcaa caaccacagc atgtgcagct
     5881 ttatgaccga agaggcgatt gagaagaaac tggcggagtt ggagtaggtc gtctgaaacg
     5941 acgatttaaa cgtaggtcgg atacttgtat ccgacagagt gggtaaaaag gcaaaatgtc
     6001 ggatttaaga atccgcccta ctgaaatact gaaatgccgt cattcccgcg caggcgggaa
     6061 tccaccggta agattcggtt tctgaattta ataagacatt gcttaccatt gaggatggat
     6121 tcccgcctgc gcgggaatga cggcagacaa gcaagtggtc gagatccaac aaaaacgatt
     6181 gaaggtcgtc tgaaaatatc gatttgataa actagatttt atttcagaag acgttacaag
     6241 ccgatacaca aagacatttt aaggtcgtct gaaacagcgg ccgcaaccga tacgaaaaca
     6301 aacaggcaca ccaaaaatgg ctatttacca atcaggcgtg atttttgacc aagtggatac
     6361 cgccaatccc gattgctgga cattggacga atacgtcaaa cgcggcggct ataccgccct
     6421 gcgtaaaatc ctgtccgaaa acatctcgca aaccgatgtg attgacgaag tcaaaacctc
     6481 cggcttgcgc gggcgcggcg gtgcgggctt cccgaccggt ttgaaatgga gctttatgcc
     6541 ccgttctttc ccgggcgaaa aatatgtggt ttgcaacacc gacgaaggcg agccgggtac
     6601 atttaaagac cgcgacatca tcatgttcaa tcctcatgcc ctgattgaag gcatgatcat
     6661 tgccggttac gcgatgggcg cgaaagccgg ctacaactat atccacggcg aaattttcga
     6721 aggttatcaa cgctttgaag ccgctttgga gcaggcgcgt gccgcaggct ttttgggtaa
     6781 aaatattttg ggttcggatt ttgaattcga actcttcgcc caccacggct acggcgcata
     6841 tatttgcggc gaagaaaccg cattgcttga atcgttggaa ggcaaaaaag gccagccgcg
     6901 cttcaaaccg ccgttccccg cctcgttcgg cctgtacggc aaaccgacca ccatcaacaa
     6961 taccgaaacg ttctcctccg ttccattcat tatccgtgac ggcggacagg cgtttgccga
     7021 taaaggtatt ccgaatgcag gcggtaccaa attattttgt atttccggcc atgtcgagcg
     7081 tccgggcaac tatgaagtgc cgttgggcac gccgtttgcc gaagtcttga aaatggcggg
     7141 cggtatgcgc ggcggtaaaa aactcaaagc cgttattccc ggcggttcgt ccgcccccgt
     7201 attgcctgcc gacattatga tgcagaccaa tatggactat gactcgattt ccaaagccgg
     7261 ctcgatgctc ggttccggcg cgattatcgt gatggacgaa gacgtgtgca tggtcaaagc
     7321 cctcgaacgc ttaagctatt tctactacga cgaatcctgc ggccaatgca ccccctgccg
     7381 cgaaggtacc ggctggcttt accgcatcgt ccgccgcatc gtagaaggca aaggccgcat
     7441 ggaagacttg gatttgctgg attctgtcgg caaccaaatg gcaggccgca ccatctgcgc
     7501 ccttgccgat gccgccgtct tccctgtccg cagctttacc aagcatttcc gtgatgagtt
     7561 tgcgcattac atcgaacacg gcgggccgat gaaagagcat aagtggggag ggtggtaatg
     7621 gtggaagcta aaatttttat tctatacggt gcagccaaca aaggtaagag tacgacactc
     7681 aatacgcttt ttaatcagat ttgtcggaaa ttttctaaat ttctagtctt ttttgaaaga
     7741 tatggaaacg gcttagattt tgttgcagta tttgatcatg aaggtcagag gattggtttt
     7801 tattcatctg gtgataatga atacgaggtt aggagaaatt tatacaaact ttattcgcat
     7861 aattgtgatt ttattttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat
     7921 attgcatggc gatgtaaata taattaattg gtgtgaaaag tttgagccta cagatgaaga
     7981 caatgagcgt gctgttaaag agttatttaa gtcatttaaa aatataataa atgagttata
     8041 gttttagttg gttttatatt ggttaaaagc aaaatgctaa aaatttaact ttgccgtcat
     8101 tcccgcacag gcgggaatcc atagtggaat ttacagaacc cgatatttga aaagcagttg
     8161 ccgaaattca aaaaatggat tcccgcctgt gcgggaatga cggcaaggtg gcagtaaacg
     8221 ttttaaacag tattgattgt caatgaaact caaaaggccg tctgaagccc atttttcaga
     8281 cgatctccat aaaaaattat ttatcaaata cccgtaacta ggaacgaacc atgttacaaa
     8341 tcgaaatcga cggcaaacag gtatctgtgg agcagggcgc gacggtaatt gaagccgcgc
     8401 acaagctcgg tacttaatat tccgcatttc tgttaccaca aaaagctctc tattgccgcc
     8461 aactgccgta tgtgtctggt ggatgtggaa aaagccccca aaccgctgcc tgcctgtgcc
     8521 acgccggtaa cggacggcat gattgtgcgt acgcattcgg caaaagcccg agaggcgcaa
     8581 gaaggcgtga tggagttcct gctcatcaac catccgcttg attgtccgac ctgcgaccag
     8641 ggcggcgaat gccagttgca ggatttggcg gtgggctacg gcaaaaccac cagccgttat
     8701 accgaagaaa aacgttccgt cgtcggcaag gacatgggtc ctttggtttc cgccgaggaa
     8761 atgagccgct gtatccactg tacccgctgc gtacgtttca ccgaagaaat cgccggtttg
     8821 caggaaattg cgatggtgaa tcgtggtgaa cactccgaaa tcatgccctt tatcggcaaa
     8881 gcggtggaaa ccgagctgtc gggcaacgtc atcgatttgt gtcccgtcgg cgcattgacc
     8941 agcaaaccgt tccgcttcaa cgcgcgtact tgggaattga accgccgcaa atccgtttcc
     9001 gcacacgacg ctttgggcag caacctgatt gtacaaacca aagaccatac cgtccgccgc
     9061 gtgttgccgt tggaaaacga agccatcaac gaatgctggc tgtccgaccg cgaccgtttt
     9121 gcctacgaag gcctgtatca cgaaagccgt ctgaaaaatc cgaaaatcaa acagggtggc
     9181 gagtggatgg acgtggattg gaaaaccgcg ttggaatatg tccgcagcgc gattgaatgt
     9241 atcgccaaag acggcaacca aaaccaagtc ggcgtttggg ctaatccgat gaatacggtt
     9301 gaagagctgt atctggccaa aaaatttgcc gacggcttgg gtgttaaaaa ctttgcaacc
     9361 cgtttgcgcc aacaagacaa acgtctttca gacggcctta aaggtgcgca atggttggga
     9421 caaagtattg aatctttggc tgacaacgat gccgtattgg tagtcggtgc gaacttgcgc
     9481 aaagaacagc cgctcctgac cgcccgcctg cgccgcgccg ccaaagaccg tatggccttg
     9541 agcgtattgg ccggcagtaa agaagaattg tttatgccgc ttctgtctca agaagccgca
     9601 catcccgacg agtgggcagg ccgtctgaaa aacctgtctg ccgatgcgga acatgccgtt
     9661 accgccagcc tgaaaaacgc tgaaaaagca gcggtgattt tgggtgcgga agtgcaaaac
     9721 catcctgatt acgccgccat ttatgccgcc gcgcaagagt tggcggacgc gaccggcgca
     9781 gtgctgggca ttttgccgca agccgccaac agcgtcggtg cggacgtatt gggtgtgaac
     9841 tcgggcgaga gcgttgccga aatggcaaac acgccgaaac aggcagtctt gctgctcaac
     9901 gtcgagcctg aaatcgatac ggttgacggt gcaaaagccg tagccgcgtt gaaacaggcg
     9961 aaaagcgtga tggcgtttac gccgtttgtc agcgaaacgc tgctggacgt gtgcgacgta
    10021 ttgctgccga ttgcgccgtt taccgaaaca tcaggcagct tcatcaatat ggaaggccgt
    10081 ctgcaatctt tccacggcgt ggtacaaggc ttcggcgatt cacgtccgtt gtggaaagtg
    10141 ttgcgcgtat tgggcaacct gtttgacctg aaaggttttg aataccacga taccgccgcg
    10201 attctgaaag acgcgctgga tgcggaaagc ctgccgtcca aactggacaa ccgcagcaca
    10261 tgggcagggg agggcgttca gacggcctca aaccgcctcg tccgtgtcgg cggcgtcggt
    10321 atttatcata ctgattctat cgtgcgccgt tccgcaccgt tgcaagaaac cagccatgcc
    10381 gccgtgcctg ccgcgcgtgt aaatccgaac acattggcac gcttgggcct gcaagacgga
    10441 caaaccgctg tcgccaaaca aaacggcgca agcgtatcgg ttgccgtcaa agccgatgcc
    10501 ggtttgcctg aaaacgtggt gcatctgccg ctgcataccg aaaatgccgc gctgggtgcg
    10561 ttgatgggca ctattgaact ggcgggagct tgattatgca ggaatggttc caaaacctct
    10621 ttgccgcaac gctcggtctg ggcgatttgg gcatcaccgt aggcttggtg gtatccgtca
    10681 tcgtcaaaat cgtgattatc ctgattccgc tgattctgac cgttgcctac ctgacgtatt
    10741 tcgaacgtaa agtcatcggc tttatgcagc ttcgcgtcgg cccgaacgta accggcccgc
    10801 ggggtctgat tcagccgttt gccgacgtgt tcaaactctt gtttaaagag gtaacccgtc
    10861 cgaagctgtc aaacaaagcc ttattctaca tcggcccgat tatgtcgctc gccccgtctt
    10921 tcgcggcgtg ggcggtgatt ccgtttaacg aagaatgggt gctgaccaat atcaatatcg
    10981 gccttttgta catcctgatg attacctcgc tgtcggttta cggcgtgatt atcgcgggct
    11041 gggcttccaa ctccaaatat tcgttcttgg gtgcaatgcg tgcttccgcg caaagcattt
    11101 cctacgagat tgccatgagt gccgcgctgg tgtgcgttgt gatggtatcg ggcagcatga
    11161 acttctccga catcgttgcc gcgcaggcaa aaggcatcgc gggcggttct gtgttctcgt
    11221 ggaactggct gccgctgttc ccaatcttca tcgtctatct gatttccgcc gttgccgaaa
    11281 ccaaccgcgc accgtttgac gtggcagagg gcgagtctga aatcgttgcc ggacaccacg
    11341 tcgaatactc cggctttgca ttcgcgctgt tcttccttgc cgaatacatt ttcatgattc
    11401 tgattgccgc gctgacatcg ctgatgttcc tcggcggctg gttgtctccg ttcccgcaaa
    11461 gctggggcat tgtcggtacg ccttccgcat tctggatgtt cgtgaaaatg gcggcggtgc
    11521 tgtactggta tctgtggatt cgtgcaacct tcccacgcta ccgctacgac caaatcatgc
    11581 gtttgggctg gaaagtgctg attccgatcg gcttcgccta catcgtggtt ttgggcgtgt
    11641 ggatgatttc accgctgaat ttgtggaaat aagtttcaga cggcatcttg aggccgtctg
    11701 aacaaagcga ttttgaatac ctaacgaaat ccctgttttg agggaacata atatggctaa
    11761 cttagtaaaa acctttctgc ttggcgaact ggtcaaaggc atgggcgtaa cgctcaaaaa
    11821 cttttttgcc cgcaaagaca caatttattt tcccgaagag aaaacgccgc aatccgtgcg
    11881 tttccgcggt ctgcacgcgc aacgccgtta tccgaacgga gaggaacgct gcatcgcgtg
    11941 taaattgtgc gaggcggtgt gtccggcaat ggcgatcaac atcgaatcgg aagaacgcga
    12001 agacggcacc cgccgcacca agcgttacga catcgacctg accaagtgca tcttctgcgg
    12061 tttctgcgaa gaggcctgcc cgaccgatgc gattgtggaa acccatattt ttgaatacca
    12121 cggcgagaaa aaaggcgact tgcatatgac caagcccatc ctcttggcaa tcggcgacaa
    12181 atacgaagcc gaaatcgcca aacgcaaagc cgctgacgcg ccgtaccgct aaggagcaga
    12241 cgaatgactt tttccgtgat tctgttctat atccttgccg ccatcgtttt gtacggtgcg
    12301 gttcgtaccg ttaccgccaa aaaccctgtc catgccgctt tgcatctggt gctgaccttc
    12361 tgcgtgagcg cgatgatttg gatgctgatg caggcggagt ttttgggcgt gacgctggtg
    12421 gtggtttacg tcggcgccgt aatggtgttg ttcctgttcg tcgtgatgat gctgaatatc
    12481 gacatcgagg aaatgcgcgc cggtttctgg cggcacgcgc ctgttgccgg tgtggtcggt
    12541 acgttgttgg cggttgccct gatcctgatt ctggtcaacc cgaaaaccga cctggccgca
    12601 tttggtctga tgaaagacat tccggccgat tacaacaata tccgcgattt gggcagccgt
    12661 atttataccg actacctgtt gccgtttgaa ttggcggcgg tattgctgct gttgggcatg
    12721 gtggccgcga ttgcattggt tcaccgcaaa accaccaatc cgaaacgtat ggatccagcc
    12781 gaccaagtta aagtacgcgc cgatcaaggc cgtatgcgtc tggtgaaaat ggaagcggtc
    12841 aaaccgcaag tcgaatctgc cgaagaaagc gaagtttcag acggcctcaa gacggaaggg
    12901 gagggcaaag catgattact ttgacgcatt atctggtatt gggcgctctc ctgttcggta
    12961 tcagcgcgat gggcatcttt atgaaccgca aaaacgtgct ggtattgctg atgtccatcg
    13021 agctgatgct tttggcggtg aacttcaact ttatcgcctt ctcgcaacat ttgggcgata
    13081 ctgccggaca aattttcgta ttcttcgtat tgaccgttgc cgctgccgaa tctgccatcg
    13141 gtttggcgat tatggtgctg gtgtaccgca accgacaaac gattaatgtt gccgatttgg
    13201 acgagttgaa agggtaatca tgaacgatat gactttatat ttgataattg cccttgttcc
    13261 gttggcaggc tcgctgattg cgggtttgtt cggcaacaaa atcgggcgtg ccggcgcgca
    13321 tacggttacg atactcggtg tcgctgtatc tgccgtgctg tcggcttatg tgctgtgggg
    13381 attcctcaat ggcagccgta ccaagtttga cgaaaacgtc tatacctggc tgacaatggg
    13441 cggcttggat ttttccgtcg gcttcttggt cgatacgatg acggcgatga tgatggtcgt
    13501 ggtaacgggc gtgtcgttga tggtgcatat ctataccatc ggttatatgc acgatgaaaa
    13561 agtcggctac caacgcttct tcagctatat ttctttgttt acattcagca tgttgatgct
    13621 gattatgagc aacaacttca tccagctctt cttcggttgg gaagcggtgg gcttggtgtc
    13681 gtatctcttg atcggtttct atttcaaacg cccgggcgcg acatttgcca acctgaaagc
    13741 ctttttgatc aaccgtgtcg gcgacttcgg ctttttgctc ggtatcggct tggtgcttgc
    13801 ctatttcggc ggcagcctgc gctatcagga cgtattcgcc tatctgccca acgtacaaaa
    13861 tgccaccatc caactgtttc ccggtgtgga atggtctttg attaccgtaa cctgtttgct
    13921 cctgtttgtc ggtgcgatgg gtaaatcggc tcagttcccg ctgcatgtct ggctgcccga
    13981 ttcgatggaa ggcccgacgc cgatttccgc attgattcac gccgcaacga tggttacggc
    14041 gggtctgttc atggtgtcgc gtatgtcgcc gatttacgaa atgagcagca ctgcgctgtc
    14101 ggtcattatg gttatcggcg cgattaccgc cctgtttatg ggcttcttgg gtgtgattca
    14161 aaacgacatc aaacgtgtgg ttgcgtattc taccctgtcg caactgggct acatgaccgt
    14221 ggctctgggc gcgtccgcct attctgtggc gatgttccac gtcatgaccc acgccttctt
    14281 taaagccttg ctgttcttgg cggcgggcag tgcgattatc ggtatgcacc acgatcaaga
    14341 tatgcgccat atgggcaacc tgaaaaaata catgccgatt acttggctga ctatgctgat
    14401 cggcaatttg tctctgatcg gtacgccgtt cttctccggc ttctactcca aagattcgat
    14461 tatcgaagca gtgaaataca gcaccctgcc gggcagcggc gttgcctatt ttgccgtcct
    14521 cgccagcgtg ttcgttaccg cgttttacgc gttccgccaa tactttatgg tgttccacgg
    14581 cgaagaaaaa tggcgcagcc tgcccgaaca ccattcagac ggccatggtg aagaacatca
    14641 cggcttgggc aaaaacgaca atccgcacga aagcccgctg gtcgttaccc tgccgctgat
    14701 tctgcttgcg attccgtccg tcatcatcgg ctacatcgcc atcgaaccca tgctctacgg
    14761 cgatttcttc aaagacgtga ttttcgtcaa cgccgacgcg catccgacca tgcacatcat
    14821 gaaagaagag ttccacggcg cattggcgat ggtgtcgcac agtctgcatt cgcccgtcct
    14881 ctatctcgct accgcaggcg tattgagcgc atggcttttg tacgtcaaac tgccgcacct
    14941 gcccgctaaa atcgcgcagg cgttccgtcc ggtttacgtt ttgtttgaaa acaaatacta
    15001 ccttgacgcc ttgtatttca acgtcttcgc caaaggcacg cgcgcattgg gaaacttctt
    15061 ctggaaagtc ggcgataccg ccattatcga caacggcatc gtcaacggct ccgccaaact
    15121 ggtcggcgca attgccgcgc aagtgcgtaa agtccaaacc ggctttatct acacctacgc
    15181 cgccgctatg gtgttcggcg tattggtatt gctcggcatg accttctggg gattgttccg
    15241 ataaggatga ggcttcagac ggcctgctta tcgctggtgt agaggtcgtc tgaaaagaag
    15301 gtttcaggcg gcaatagatt agagtttcag acgacatctg acatttcaga tagtcaggcc
    15361 gtctgaaaaa tagaagggcg gataatatgg ataaacggct gtttgacggt acggttcgtt
    15421 ttgagcatgt aaccggtgtc ggcacgatgc aggtagattt ccagcccaat cagcgtgttt
    15481 atacgatgat tggcgagaat ggtgtcggta agaccaaatt tttggaatgt ttgtttacgg
    15541 tattgttgtt tacttatcga atggagaaca taaacatagg ttttagttgg atagatttgc
    15601 aacgtttacc tttgaaaata gttgaattgc ctaatggaaa aaatgaggat tttaggcgct
    15661 tttcagagcg tagtaatcag ggctttaata ttaacgaaga cgttttgttc tcaggatatc
    15721 aacatcgact tccttttgta tatttgccgg cacagagtcg tgggtgggta aaaaaaacaa
    15781 acttccaaac tcagtcgctg ggaagttctc aagggcgtgc aagcaaatat ctggggaatt
    15841 tgttttctgt ttttcaatct ggccatagtt cattctcgct taaatcctta aatatggaaa
    15901 acgatttaga agaatggatt atccagcgcg cccgatcagc caatccgtat caggcaaacg
    15961 aagataatcg aaaaattgaa attgatactt tactgtttct tcttaatcaa tttgatggtc
    16021 gtattgcacc tgatttttta gaaattaaag gagatgatcg tatattcatc aagttggata
    16081 atcaaaaacg cgaattgtca ggattatcca gcgggtttac gtctattttg aaaattatgc
    16141 aatctattgt tgcgggatac agttacttta ccaatgagca gcaaattgcc aatgtgcgcg
    16201 gcgtggtgct gattgacgaa atagaaagcc atttgcacaa cgaatggcag atcaaaatcg
    16261 tgccgctgct gaaaaaattg ttcccgaaca caacgttttt cattaccacg cattcttctt
    16321 tagtcatcag tcagttagag cagggagggc ctatcgtttg gtacgtagca aggaggacgg
    16381 tgtagtctat ggcaagatga ttgactatcc aagtaatgct tcttttgtgg atttgctcaa
    16441 cgaagcattc ggcgtggatt tgaataagaa gaagatagaa cgcgcccaag aacgtaatca
    16501 agaacaggca aaaaagcctt attggaattg gtacgtcagg aattgggcgg attaggagat
    16561 aaataatgac ggtttatctt ttagatacca attacttggt ttatttggcg gatgatgaca
    16621 gtgacgaaga aaagcggaaa gccgtattga gcgatatggc ggaaaagttg cagcaggatg
    16681 acaaccgttt tgtgattacc ccgcttatcc gttatgaagt tttgcgcggt gtggattggg
    16741 gaaaatctga aaaattgtct cgattgaccg gtgttttggc acagttttag tctttggaca
    16801 ttacacagaa tgtttctgat ttggcaagta atctgtaccg ttttgataag tttgaagccg
    16861 aacgtgataa cacgccgaag aatttagaaa aacgtaaatt cgatatgttt cattatgcga
    16921 ctgccagtgt gaataattta gaaatcttat cccacgatac cgatgttaat aagattaaag
    16981 atttacatga aagaatgagg ctggaagatt cggcagaatt agcttgagac aaatagcaga
    17041 aacctgaaca aacctttttc agacggcctc ttaaaacgtc gtctgacaaa ccaacccgta
    17101 caacgatatt tttattttaa ccacaggtta accactatgt tttccaacta cctactcagc
    17161 ttggcaatat ggatacccat cgccgcaggc gtgctggttt tggcgaccgg taaagacagc
    17221 cgtgcgccgc tggcgcgtgt gcttgccttc atgggagcgc ttgccggttt cttggtaacg
    17281 ctgcccctgt ttaccggttt cgaccgtttg agcggcggct atcaatttac cgagttccac
    17341 gagtggattc cgcttctgaa aatcaactac gcattgggcg tggacggtat ttcagtgctc
    17401 tttatcatct tgaatgcgtt tattacgctg ctggtggtat tggccggttg ggaagtcatt
    17461 cagaaacgtc cggcgcagta tatggcggca ttcctgatga tgtcgggttt gattaacggc
    17521 gcgtttgccg cgcaggatgc gattctgttt tatgtgttct tcgagggtat gctgattccg
    17581 ctgtacctga ttatcggtgt atggggcggc ccgcgccgcg tctatgcgtc ggtcaagctg
    17641 ttcctctata cgctgacggg ttcgctcttg atgttggttg ccatggttta cctgtactat
    17701 cagacaggca gcttctctat tgtcgatttc caaaacatca aacagattcc gttgggcgta
    17761 caacagcttt tgtttgtagc gttcttcctg tcatttgccg taaaagtgcc gatgttcccc
    17821 gtacacactt ggctgccgga tgcccacgtt gaagcgccga ccggcggttc gatggtgttg
    17881 gcggcaatta cgctgaaact gggtgcgtat ggtttcttgc gctttatcct gccgattatg
    17941 ccggacgcgg ctcgctattt tgcccccgtg atcatcgtat tgagcctgat tgccgtaatt
    18001 tatatcggta tggtggccct ggtgcaaacc gatatgaaga aactggtggc gtattcgtcc
    18061 atcagccata tgggttttgt aacgcttggg atgtttttgt ttgttgacgg gcagttggac
    18121 gactgggcgt tgaaaggcgc ggtcattcag atgatttcgc acggcttcgt gtcggcggcg
    18181 atgtttatgt gcatcggtgt gatgtacgac cgcctgcaca cgcgcaatat tgccgattac
    18241 ggcggcgtgg tcaatgtgat gcccaagttt gcggcgttta tgatgttgtt cggtatggcg
    18301 aacgccggcc tgcctgcgac ttccggcttc gtgggcgagt ttatggtggt tatgggcgcg
    18361 gttaaagtga atttctgggt gggcgcgctg gcggctatga cgctgattta cggcgcgtct
    18421 tataccctgt ggatgtacaa gcgcgtgatt ttcggcgcga ttcacaatcc gcacgttgcc
    18481 gaaatgaaag acatcaattg ccgcgagttt gcgattctgg cggttttggc ggttgctgtt
    18541 ttggggatgg gcctgtatcc gaacgcattt atcgaagtgg tgcatcaggc ggcaaacgat
    18601 ttgattgccc atgtggcgca aagcaagatt tgaggtgtgt aaatgaattg gtcagatttg
    18661 aatttaatgc ccgcactgcc cgaagtcgcg ctgctgtcgc tactggtgtt attgttgccg
    18721 gcggacttgt gggccagtga tgacaaatgc cgttggacgc attacggcgc gttggcaacg
    18781 gtggcggtta cggcggcggt gcagcttgcc gtgtgggaac agggcagcac gtcttcgttc
    18841 aacgggatgt atattgcgga cggtatgtcg cgtttggcaa aaatggtttt atatgccttg
    18901 acgtttgtcc tgtttgtcta tgccaagccc tacaaccaag tgcgcggtat tttcaaaggc
    18961 gagttttaca ccctgtcgct gtttgccctc ttgggcatga gcgtgatggt gagcgcgggg
    19021 cattttttaa ctgcctatat cggtttggaa ctcttgtcgc tcgcccttta cgccctgatt
    19081 gccctgcgcc gcgattccgg ttttgccgcc gaagccgcct tgaaatattt tgttctgggc
    19141 gcgctggcat ccggcctgct gctctacggt atttctatgg tttacggcgc aaccggttcg
    19201 ctggaatttg ccggcgtgct cgcctcttcc ttcaatgaag aagctaacga atggctgttg
    19261 aaactgggtc tggtgtttat cgtcgtcgcc gttgcgttca aactcggcgc ggtaccgttc
    19321 catatgtgga tgcccgacgt gtatcacggc gcgcccactt ctgttaccgc cttggtcggc
    19381 actgccccga aaatcgccgc cgtcgttttc gctttccgca tcctcgttac cgggctggga
    19441 accgtgcatc atgactggtc tctgatgttt gccctgcttg ccgccgcctc gctgctggtc
    19501 ggcaaccttg ccgccatcat gcagaccaat atcaaacgta tgctcgccta ttccaccgta
    19561 tcgcatacgg gtttcatcct gttggcgttt atggcgggcg cggtcggctt tgcggcgggc
    19621 ctctattacg ccattaccta cgcgctgatg gcggcggcag ggttcggcgt gttgatggtg
    19681 ttgtcggacg gggacaacga gtgcgaaaac atcagcgatt tggcagggtt gaaccaacac
    19741 cgcgtatggc ttgccttttt gatgctgctg gttatgttct ctatggcggg cattccgccg
    19801 ctgatgggtt tttacgccaa attcggcgtg attatggcgc ttttgaaaca aggctatgta
    19861 tggttgtcgg tgtttgccgt cgtgatgtcg ctggttggcg cgttctacta cctgcgcgtg
    19921 gtcaaagtca tgtattttga cgaatccggc cgcgcccgtc ccgccgccgg cggcaacaat
    19981 gccgcaaaat ccctcttaag cgtcaacgcg ctgctgttgg tgctgtgggg cattatgccg
    20041 caaaccgtta tcgactggtg cgccaaggcg ttggagaaca cgctgtgagc cgccgaaacg
    20101 gcagccgtgt cagaggctgc cgtttttgtt aagatatgcc gttccgcaat gcagttcaga
    20161 cggcatcgcc gccgacaatg cccaaacaga aagcccatca tgaccgcatc catgtacatc
    20221 cttttggtct tggcactcat ctttgccaac gcccccttcc tcacgaccag actgttcggc
    20281 gtggccgcgc tcaagcgcaa acatttcgga caccacctga tcgagctggc ggcaggtttc
    20341 gcgctgaccg cctctcttgc ctacatcctc gaatcccgtg cgggagcggt acacaatcag
    20401 ggttgggagt tttacgccac cgtcgtctgc ctgtacctca ttttcgcctt cccgtgtttc
    20461 gtgcggcggt atttttggca cacgcgcaac agggaataaa caagcaaaca agcataggaa
    20521 tgccgtctga aaccctttca gacggcattt gtttcattca agtgcaggcc ggcatcgctg
    20581 tgccggcacg tttcagtcgg cgatatacgc cggttttaat atttgcgtgc gactgcaaat
    20641 tctgccaact gccgcaggcg cagggctttg tcgccgaagg gttcgagcag cgcgactgct
    20701 tcggcaacca gtttgtgtgc gtatgagcgc gccgcttcca agcccatcag tttcacataa
    20761 gtcggcttgt cgttgtcggc atctttgccc gccgtcttgc ccaaagtcgc cgtgtccgct
    20821 tcacaatcca acacatcgtc gatgacttgg aacgccaaac ccagttttgc cgcgtaagcg
    20881 tccaatacgg caagttccgc gtcggacaga tcgggacacg ccgtcgcccc caataaaacc
    20941 gccgcacgga tcaacgcccc cgttttcagg ctgtgcatcc gttccaaatc ggcttgaacc
    21001 atttgtttgc cgacatttgc caaatcgatt gcctgtccgc ccgccatacc cgcgctgccg
    21061 cccgcttttg ccaacaccga caacattgcc aactggcgtg cggcgggcag ttctgtcgga
    21121 cggctcaaca cgtcgaaggc ttgggtttgt aaagcgtcgc cggtcagaag ggcggtggct
    21181 tcgccatatt tgatgtggca ggtcggtttg ccgcgccgca ggctgtcgtt gtccatcgcc
    21241 ggcatatcgt cgtgaaccaa agaatagacg tggatcattt cgattgccgc cattgcctgt
    21301 ccgaccgcat ccgccacggc cccgcccaat tccgaagccg ccagcaccag catcgggcgc
    21361 agacgcttgc cgccgtccaa agccgcatag cgcatcgctt cgtgcagcgt gtgcgggatt
    21421 tcgtttccag acggcaaaaa gcgttccagc agcagctctg tctgcgcctg cgccctctgt
    21481 tgccacgctt tcaaatcatt cgtcggattc aaggttcaac tccttcgtcc cgtccgcatc
    21541 caaaacctgt aatttctgtt cgacttgggc cagcttggtt tggcagtacc tgaccagctc
    21601 gttgccttcc tgataggcgg caagcgcgtc ttccaagggc atttcgccct gcatggactg
    21661 cgtcagcgat tcaaggcgcg acaaggcctc ttcaaacgat ttcggggtgt ttttcttcat
    21721 agtttcgggt gttccggtaa aaagcggatt gtagcatttt tgcaaaacgg atgccgtctg
    21781 aaacccgaat ccggcttcag acggcatttt ttccgccccg gtcggcaagg cgttacccga
    21841 gcagttcgtc ggtgatgccc tgcaaaaagg cgaggcgttc ggggcttgcc gccccggttt
    21901 cggcggcggc tttgaaggcg cagccgggtt cggcgcggtg ggtgcagttg tggaagcggc
    21961 attgcccgac aaggtggcgg aaatcgggga aatagtgcgg caaatcggcg gcttggaggt
    22021 ggtgtaaacc aaattcttgc aaacccgggg agtcgatgag ttgggtttcg ccgttcaaat
    22081 cataaagccg ggcgtgggtg gtggtgtgtt ttcccgagtc gagtgcggcg gaaatgtcgc
    22141 cggtgcgggc ggtttggctg cctaaaaggg cgttggtcag ggtggatttg cccataccgc
    22201 tttgtccgag caggatgttg ctgtgccctt gcaggacggg gcgcaggctg tcggcgtttt
    22261 ccagcacgcg ggtttcgatg acggggtaac ccagcgtttc gtagaatttg agtttttcga
    22321 gccagagggc ggtttcgggc aggtcggctt tgttcaggac gatgacggcg cggatgccgg
    22381 cggcttcggc ggcaagcagg gcgcgctgta gcagccgcac gctcggactc gggacggcgg
    22441 cggttacgat gaggagttgg gtaacgttgg cggcgatgag tttggttttc cacgcgtctt
    22501 ggcggtagag caggctttgg cgcggtaaaa aatcttcaat cacaacttgt tcggcgttga
    22561 cggggctgat gcggacgcgg tcgccgcagg cgaaatcgac gcgttttttg cgggtgctgg
    22621 cttcgtaggt tgtgccgtcg ggcgtgcgga cgatgtagcg gcggccgtag ccggcggtaa
    22681 tttgggcggt gtcgttcatg gtttctttgg ggttgggtgt ggaaatgccg tctgaaaagg
    22741 ggtgttcgga cggcatcggt tcagtcgtgc tgccactcga cgtgttcgct gaggaagccg
    22801 ccgctctggt gcgcccagag tttggcgtaa agcccgcgtt tttcgaggag ttcggcgtgt
    22861 gtgccttctt cgatgatgcg gcctttgtcg aggacgacga gcctgtccat cgcggcaatg
    22921 gtggagaggc ggtgggcgat ggcgatgacg gttttgcctt ccatcatttt gtcgaggctt
    22981 tcttggatgg cggcttcaac ttcggaatcg agcgcgctgg tggcttcgtc aagcagcagg
    23041 atgggtgcgt ctttgagcat cacgcgggcg atggcgatgc gctggcgttg tccgccggag
    23101 agtttcacgc cgcgttcgcc gacgtgcgcg tcgtagccgc tccgcccttt ggcatcggaa
    23161 aggtcgggga tgaagccggc ggcttcggcg cgttcggcgg cggaaaccat ttcggcatcg
    23221 gtcgcgtcgg ggcggccgta aataatgttg tcgcgcacgg aacggtgcag cagcgaggta
    23281 tcttgtgtga ccaaaccgat ttgggcgcgt aaagattctt gggtaacgcc gcttatgtcc
    23341 tgcccgtcga tcgaaaccgt gccgctttgc ggttcgtaga aacgcaaaag caggttgacg
    23401 atggtggatt tgcccgcgcc gctgcgtccg atcaagccga ctttttcgcc gggtttgata
    23461 ttcaggttaa agccgttgag cagcggtttg ccggcttcgt agcaaaaatc gacgtgttcg
    23521 aacttgattg cgccttgcgg cacgttcagc ggcagggctt ggggcttgtc gaggatggtg
    23581 tgcggtttgg acagggtcgc catgccgtcg ttgacgatgc cgatgttttc aaacagccgc
    23641 gcggattccc acataatgta ttgcgacagc ccgttgacgc gcaacgccat cgcggtggcg
    23701 gtggcgaccg cacccacgcc gacctgtccg ttgtgccaaa gccagatgcc cagtgcggcg
    23761 gcggagaggg tcagcgaagt gttgacgatg aagctgcacg aatgcagcag agtcgccagc
    23821 cgcatttggg cgcgcaccgt aaccataaat tcttccatcg actgcttggc atagacggct
    23881 tcgcgcgcgc cgtggaaaaa gagtttgacg gtggcgatat tggaataggc atcggtaatg
    23941 cggccggtca tcagcgagcg ggcattcgcc tgccgtgcgg cggtttgccc caatctggga
    24001 atcagcaggc gcattaccga agcgaaaccg attatccagc cgataaaggg cagcagcagc
    24061 catgagtcga gcgaggcgag aatcacgccc gaagtgatga aatacaccga cacataaacg
    24121 accatatcgg caaccgtcat caccgcgtcg cgcaacgcca gcgcggtctg catgactttg
    24181 gcggacacgc ggccggcaaa ttcgtcctga taaaaaccga ggctctggtt cagcatcagg
    24241 cggtggaagt tccagcgcag gcgcataggg aacacgccct gaagggtttg caggcgcacg
    24301 ttggacgcgg caaacgccca cacgaccgaa aacgccatca tcgccgccat tgccgccagt
    24361 tcccaacctt tttcggcaaa cagttcggcg ggcgcgtatt tgccgagcca ctctacgatt
    24421 ttgcccataa attgaaaaat cagggcttcc ataatgccga tgccggcggt cagcgcagct
    24481 agggcggcta tccatttccg caccccgtcc atactgctcc agacaaaccg gcacaagcct
    24541 ttttcgggcg ttttcggcgc ggcttcgggg taggggtcga ttcgggactc gaaccaggaa
    24601 aatattttgt tcaacattgt tttcgatttc ggtaaaacag tttcagacgg catcaaacac
    24661 aatgccgtcc gaaaggaggg acaataacgc cattttacgg gaaaagccgt cgggaagaca
    24721 gcgcggggcg gaaacgcagg gtttcgtcag ggcaaacgcc gcgccgcctt cggacggcat
    24781 tatttcagca ggtttttcag ggcaaggcgc acgccttcgc ccacgtccgt ccccttcgga
    24841 acgcctttga ccgccgcttt tgcctcgcgt tcgttgtaac ccagcgcaag cagcgtgctg
    24901 acgatgtctt ccgtttcgtc ggcggcgggt gaggcggcaa acagcccgtc cgttaccgta
    24961 tgcgcgacca gcttgccgcg cagttccaag accatacgtt cggcggtttt tttgccgatt
    25021 cccggagcgg aggagaggcg tttgacatct tcttctgcaa ccgcccgcgc cagctcgtct
    25081 gccgtcatcg ccgacaaaat gcccaaagcc gttttcgcgc cgatgccgcc gaccttaatc
    25141 agttggcgga aggtcttgcg ttcttccgca gtggcaaaac caaataaaag atgtgcgtct
    25201 tcccgaatga taagctgggt aaacagttgt acgctttcac ccacgggcgg caggttgtag
    25261 aaggtctgca tcgatacgtc ggcttcataa ccgacaccgt tgacatcgat gacgatttgc
    25321 ggagggtttt tttcaaccag tttgccggtc agtctgctga tcatgtgtgc cgaatcctga
    25381 agtgtcgggt gcaaaatgcc gtctgaaacc ggttcgggct ccagacggca cggattgtat
    25441 caaattcagt cgtcgcggcg ggaggaaatc acgcggccgg tacgggcatc gacaacgact
    25501 ttgtattcct gtccgttttt gacgatttcg acatcatagt gcggacggcc gtcgtcgtgt
    25561 tcgagatcga tgtcggtgat tttgccgccg acacgcgccc aagccgcttt ttcggcttgg
    25621 gcgcggctga tgattttgtc ttgtttgccg tgttggtgtg cggcgtgtcc gtggtcgtca
    25681 tcgccgtgtc cgtcgtggtg ggcgagtgcg ggggcggaaa tgcccaacag tgcggttgcg
    25741 gcggcggtca agagaaggtg tttgatgttc atatttgccc ttgtaaatca ggggttgaaa
    25801 aatggggata ttaataaggt atcgaataac cgtcaaccgg aggtcagtac cgcccgaacc
    25861 ataccgcgtg cctgcgcttc ggcttcggcg gcgcgttcct gcgaggcaaa cgggcccatt
    25921 ttgacaacgt attcgtaacg gcgtttttca accgagaggt tcgggcttga tgacgaagcg
    25981 gcgaaatttt gggcggcttg gttcagatag gcttgtgctt cgtgttccgt accgaaagat
    26041 ttcaagtcga taaagatgtc tttgttttcg gcaaccggtg cggattggcc cgggacgatt
    26101 tgttcgattt tgacgtgtgc cgtcccttgg ctgacaaagc ccaatttttg cgcggcggct
    26161 ttggatacgt cgatgatgcg gttgccgtgg aaggggccgc ggtcgttgac gcggacgatg
    26221 acgcttttgc cgtttttggt gttggttacg cgcacatggc tggggatggg cagggttttg
    26281 tgggcggcgg taaaggcgtt catatcgtag cggtctccgc cggaagtttt gcgcccgtga
    26341 aacctgccgc cgtaccacga ggcgttgccg gtttgcgtga attcggcgac tttgtagctg
    26401 cggttggcgg aggcgtgcag tttttccgcc ttgaccactg cgtcggcgga tgccgtctga
    26461 agggagtgtg tgccgaacgc ggcggtgagg aggaaaaggg tttttcgggt taaagtcaaa
    26521 atgtgttccg ttcttgagtt gaagacggat gggcattatg ccctacggac gcgttccgaa
    26581 ccgccgcacg ctgcggcagc ggttcggaat gtgtccgaac ggttttcaga cggcatgaac
    26641 ctgcgttcaa acgccgcctg cgtaaccgtg ttgccgccac gcttcaaaga gaatcacgga
    26701 gacggtgttg gaaaggttca tactccggct gccgggccgc atcggcaggc ggattttttg
    26761 cgcggcgggc aggctgtcga ggatgtcggc aggcagcccg cgcgtttccg gcccgaacag
    26821 taaaacgtcg cctttttgaa acgcggtttc atcggggcgc gccgtgcctt tggtggtcag
    26881 ggcgaaaata cgtcggcctg ccagcgactt gaggcagtcg tcgaagtttt cgtgcaccgt
    26941 caggctggcg aattcgtggt agtcgagccc ggcgcgtttc attttggcgg aatccaatgg
    27001 gaagccgagc ggtttgacaa ggtgcaaatc cgcgccggta ttggcgcaca ggcggatgat
    27061 gttgcccgtg ttcgggggga tttccggctg atataaaacg atggtaagca taaatatcaa
    27121 tcacttatag gcgcgtaacc ttgccataag gcggatgggg tgtcaaaaaa tttagttatt
    27181 ttttcattgg cgtgcgtgcc agcgtccagc agcagattcg gtttgcgccc gattttttca
    27241 gcgtctttgc caattcgtcc agcgtcgcgc cggtggtaaa gacatcgtcg attaacaaaa
    27301 tattacagtt ttccggtatc ggtgtgcgga tttcaaaggc gtttttgatg tttcgccgcc
    27361 gttcgccgcc tttgagcgtg ctttgcggcg ggcggtggtg tcggaatacg gtgtgtcggg
    27421 gcagtatctg ccagccgtag cgttgcgcca acaacccgac gatgctctcg ctttggttga
    27481 acccgcgttg cagcagccgc tccctgctta gcggaacggg taggacgaaa tcgaaacatt
    27541 cgtctgaaag ccggtcgggc ggattctgca tcgtcaggtc tgccagcggc tgcgccatgc
    27601 ccaaatcagc caagtgcttc agcgcgcgta tcatattgct gacgggcggt tcgtaatgca
    27661 gcgatgccca catccggtcg aatgcgggcg gttttttctg acatccgccg cacaccgcgc
    27721 cgccttgaac gtgcctgaaa cacaggggac agctgtttgc cgcgtcggtg cggaaacttg
    27781 ccaaatcggt attgcagccg gcgcagatgc cgtctgaaac gccggacgaa ccgtggcata
    27841 atacgcaacg cctgatagtg ggcgcgtctg cgatgcgccg ccaacaagag agaaaattca
    27901 tgcctgatgc tgccaaaaaa gtttacctga tacacggttg gggggcgaac cgccacgcgt
    27961 tcgacgattt gatgccgcgc ctgcccgcaa cgtggccggt gtccgccgtc gatttgcccg
    28021 gacacgggga cgcgcccttc gcgcaaccct tcgatattga ggcagctgcc gacggcattg
    28081 ccgctcaaat cgatacgtcg gccgacattc tcggctggtc actcggcgga ttggtcgcgc
    28141 tgtatctggc ggcgcgccat cccgacaaag tccgttcgct ctgcctgacg gcgagtttcg
    28201 cacggctgac ggctgccgaa gactatcccg aagggcttgc cgcgcctgca ctgggcaaaa
    28261 tggtcggcgc gttccgaacg gattatgcca aacatatcaa acagtttcta caactccagc
    28321 ttctgcacac gcctgatgcc gccgaaatca tcggcagaat cctgcccgat ttggcgcgct
    28381 gcggcacgcc tcaagcctta caggaagctt tggacgcggc ggaaagggcg gatgcgcggc
    28441 atttgttgga caagatagat gttccggtac tgctggtgtt cggcggcaaa gacgcgatta
    28501 caccgccgcg tatgggcgag tatctgcatc ggcatttgaa aggaagcagg ctggttgtga
    28561 tggaaaaggc ggcacacgcg ccgtttttga gccatgcgga agtgtttgcc gcgctgtacc
    28621 gcgactttgt cgaagggggt ttgacatgaa tccgcaggac aaatgctggc aggttcaccg
    28681 ccatcttgcc gaacataccg accaacgcct gacactcgtc cgcaacgcgc ccgggcatat
    28741 cctgcttgcc ggtgcggatg cggacatcag ccgcagcctg ttggcgaagc gctatccgct
    28801 ggcggtattt gaagaatacg attcccgtgc ggattttttg gcggctgccg cagccgcgcg
    28861 caagggcggt ttctggcaaa aactgacggg caggggcgtg gtgcagcact gccaatcccc
    28921 gaccgtgccg ctgcccgaag cgtgtgccga tatgttgtgg tcgaacctcg gactgttggc
    28981 ggcggaacaa atccttcccg tgctgcacaa ctgggcgcgc gcgctgaaga cggacgggct
    29041 gctgtttttc acctgcttcg ggcgcgatac cttggcggaa ctgaaatccc gtctgaaaga
    29101 aaacggcatt gaaagccgca gcgtgatgtt tcccgatatg cacgacttgg gcgatatgct
    29161 cgccgaaaac ggcttttacg atccagttac cgatacggcg aagctggtgt tggactacaa
    29221 aaaggcggaa acgttttggg cggatatgga cacgctgggc gtttggcggg cggtggcgtg
    29281 ggacgacgaa aacgccgcgc gttcgtgtgc cggggcgata tttgaacggg aaggcggttt
    29341 gggcattacg ctggaaacgg tgtacggaca cgccgtgaaa aaactgatgc tgccgcaggg
    29401 ggagaacgtg gtgcgttttt ttccgaagag atgatgtgca gatgacgtct gaagccgttt
    29461 ccaagtttca gacggcattt gtctgtgaaa accgacagaa ataaaggaaa tgccgatgta
    29521 tagtgaatta aatttaaacc ggtacggcgt tggctcgcct tgccgtacta tctgtactgt
    29581 ccgcggcttc gccgccttgt cctgatttaa atttaatcca ctatgtgccg atgccggaga
    29641 cgtatattgc gtctataaca tcagactgaa gcagtacact gcctgccagg ttacccgagt
    29701 tgaagaacac ggtggcaaaa aaacacatgc gaccctgctg gctttggact ggcggggcaa
    29761 caaaccgctt ggggcggcgg agctggcgga tttgaaaccg ctttacaaag actttatgta
    29821 ttgggaacgc ggtctgcaca tgtataaggc aagtgccgtc gtgccgacgg gatatgtacg
    29881 ggttgggaat accgcgccgc tgtgcggcga agacacgcag cggtatgcct ctttttgggg
    29941 cgacggctac gacgtgtacc gtcagttgag atggcggcag atacccgaaa aacagagaaa
    30001 ggcattcaaa aaagccgcca aaagcaaaaa taccgtgatg tttgccggac gggaatacgg
    30061 catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga tggaactgaa
    30121 ggcgtttccc tgcctgtctt cgctgtttct gaccaagtgg cataaaaatc tatatgaata
    30181 tctggaggaa tatccgttta tcacccgatt gtgtctggaa aaccacggtc agaccgtgct
    30241 ggatttcagc aatacgcgca taaccgacct ttctgtcgat atgaccggcg tggaatcgct
    30301 ttatctgaac gaaggcttgg acagcctgaa cctgaaaggc gaaatcaagg aaaactgcaa
    30361 ggtctgtacc gccggaaagg gtgcgggtct gattttggaa gtgggcaaaa gcgttcccaa
    30421 agtgcggggt ttggaaaacc tgaccgccgt caacgtcatg ggcattgccg atttcgatat
    30481 gcaaaacctg tcggaaacgt atcccaagct caaaaccata cggctgtggg gcaaaccggg
    30541 caatatcgcc aatttttccg ccgtatcggg atttgaggat ttggaagtgt tcactgccgt
    30601 agatttgttc ggtttcggcg cggacgacat ccctcatccc gaccggctgc cgaagctgca
    30661 caggctgtgg atgagcagcc tgcccgaaga ggcggcgaag gcagtgaaga aactttataa
    30721 gaaacggaag gaagacgggc tggacctgtg gatagaaaaa gcccgaaaac ccgaatggct
    30781 ggcgcaaaat ttcgacaacc ccttccgcga ttgggacggc gcggagcata tcccgaaaag
    30841 ccatgccaaa aaagcggcgg agctgtaccg gaaaacccgt gccggcgtgg tcaaactgct
    30901 cggcaatccg cctgaaaaca caggggaggg gctggcagag gcggtcaaag cctataccgg
    30961 aggtttcaac aaaatggaca aaaaacactt tatcgacacg gtagagcggg aagatattgc
    31021 cgaggcgttg gaaacgatat tggacctgat accggatggg tcttgtgccg acaaggaaaa
    31081 actgtttgaa atatttgata aaaacaggaa tttttaaaca gaggcaatgc cgtctgaaac
    31141 ttggtaacgg gcttcagacg gcatcccgtt ccaacaccgc caacaccgcc gcaccgtaac
    31201 gtgcggcttt ttcttcgcct acgccgtata cggcggcaag ctccgccaaa ccttccggct
    31261 gtttggcggc aatggcgcgc agggcggctt tgctgagaat gcggtagggt tcggactgtt
    31321 cgtgttttgc ggtttcgttg caccattgaa tcagggcgcg catcaggcag cgtttgtgtt
    31381 tggcggtttc atcgatgccg tctgaaaacg gacggcagac ggcgaggatg tcccgtccgt
    31441 atttggcggc gcgtacgctg cccaagccgt gtacggcttc gaggtcggtt tcggtttcgg
    31501 gcgtggcggc aagcatatcg gcaaggcttt cgtcggagag gacggtatgc agggcgcagt
    31561 tttccgccct tgcctgttca taccgccagg cttcgagttt ttgacgcagt tgttgttcgc
    31621 gttcggtttg cggacggatg acggcatcgt ggctaaagtc ggcagcgttt tggcagactt
    31681 tgagtatacc gtgtccgaaa cgttcggttt tggcttcgcc caagccgtag atgtcgtgca
    31741 gcccgttgag gttttgcggc attttttcga caaggtcgcg cagggttttg tcgccgaaaa
    31801 tcatataggc ggggatgcct tcggcttcag cctgtttcat gcgccaaacg cgcaatgcct
    31861 gccacaggcg ttcttcgcgt tcggtacgca gccagttgtc tttgagggtg cgggtggcgg
    31921 gcttgtcgcg cttgagcggg cgcagcatca cttcggtttc gcctttgagg atttttttgg
    31981 cggcttcggt cagttgcaat gcctgatatc gggcaatgtt gacggtgagg tagccgaggc
    32041 tgatgcactg gcggatgacg ctgcgccatt ctttgtcgga caattccgcg ccgatgccga
    32101 atgtggacag ttgctcgtgc cggttgccgc gtatccaatc gtcgcttttg ccgcgcaaaa
    32161 ggttggtgat gtaaccggcg gcaaagcgtt gtccggcgcg gtacacgcag ctgagtaatt
    32221 tttgcaccaa caccgtgccg tcaaaccgta cgggcggatg caggcagttg tcacaatgtc
    32281 cgcagggttc ggatgcttcg ccgaaatgtt tgagcagcag tacgcggcgg caggcggcgg
    32341 tttcgcagac ggaaagcatg gcatcgagtt tttgcatttc gatttgcttt tgcacctcgt
    32401 cgctgttgcc ttcggcaatc cgttcgcgca gcaacaccca atcgttcaag ccgtaacaca
    32461 gccaactcac ggcaggcagc ccgtcccggc cggcgcgccc tgattcctga tagaaatgtt
    32521 cgacactctg gggcatatcg agatgggcga caaagcgcac gtcgggtttg tctatgccca
    32581 tgccgaacgc cacggtcgcc accacgataa tattgtcttc atgcgtaaag cggcgttggt
    32641 tttcctcgcg cacgtccatg ctcaaaccgg catgatacgg aatcgcgttt aatccgtttt
    32701 cacgcaaaaa ctgcgccgca tcttcaacct ttttgcggct taggcaatac acaatgccgc
    32761 tttgccccgt catttctttg cggatgaaat ccagcaattg ttttttgccg ttgttttttt
    32821 cgataacctg ataataaata ttcgtgcggt caaagctgga gacaaattcg gacgcgtcgt
    32881 ccaagtgcag ataatgcttg atgtcggcgc gcgtggcggc atcggcggtg gcggtcaggg
    32941 cgatgcgcgg gatgttcgga tagcgttcgg caagcatacc gagctgttga tattcggggc
    33001 ggaaatcgtg tccccaccgg ctgacgcagt gcgcttcgtc aatggcgaac aggctgacgg
    33061 tttgttggtc gagaaaacgt aaaaagcggt cggtaaccaa gcgttccggc gcgacataaa
    33121 gcagcttcag acggccttgg gcaagcttgt cggcaatctc gcgcgcctca tctgccgatg
    33181 tgccgctgtt gactgccgcc gcttcaatcc cggccacatg caggctggcc acttggtcgt
    33241 tcatcagcgc aatcagcggc gatacgacaa ccgccacgcc ttcgcgcatc agcgccggaa
    33301 tctggtaaca caaagacttg cccccgcccg tcggcatcag caccgtcaaa ctgccgccgc
    33361 ctgccaaagt attgatgaca tcctcctgcc tgccgcgaaa ttcgggatag ccgaatactt
    33421 cgtgcaaaat ctgtctggcg gtgggtcggt gcgtcattgt tccgtgtccg gtaagggtgt
    33481 tggtcggtcg gcggcaatat gccgtctgaa atcgggattt tagaagagtt tgcccacttc
    33541 cgccccaata tcgtcggcac gcataaacgt ttcgccgatc aggaaggtat gcacgccgcg
    33601 cgcccgcata aattccacat ccgccttgcc tgtaatgccg ctttcggtaa cgacggtttt
    33661 gccttccagc gcgggcagca gcgacagggt ttggtcgagg gagacttcaa aagtccgcag
    33721 gttgcggttg tttacgcccc gcaacggcgt ggtcaggttg cggcattttt ccaattcggt
    33781 ttcgtcgtgc agctcgagca ggacggtcat gcccaattcg tgcgccagcg cctcaaagcg
    33841 ttccaattgt ccctgttcca gtgcggcggc aatcagcagg acggcatccg ccccccatgc
    33901 gcgcgcctga taaacctggt attcgtcgat gatgaagtct ttgcgcagca cgggcagcaa
    33961 tacggcttcg cgcgcctgtt tgaggtattc gggcgaacct tggaaatagg gttcgtcggt
    34021 cagtaccgac aaacacgccg ccccggcgtt ttcataggcg cgggcaatct cggcagggcg
    34081 gaagtcgggg cggattaacc ctttgctcgg gcttgccttt ttgatttcgg caatgacggc
    34141 gggcaggttt aggcggtgtt tgccgcgtat cgaatcgatg aagctgcgga cgggcgcggc
    34201 ttctgcggca agtgcgcgga tgtgttcggc gttgacggcg gctttttggg cggcaacttc
    34261 ctgtgccttg gtggcaagga ttttattgag gatgtcggtc atgtcgtgtt ccgtatttgt
    34321 ccggggaaag ggggaatatt agcatcaaac cgttaacgcc tgtttgtgcg gaagctgtca
    34381 aaataggaca ggacggtctg cggcagccat tgcaggtgca gcctgccgcc ggtgctgctg
    34441 acaaagccgg cgtgcccgcc gtgtgcaggt tggaacaggg taacggcttc ggacgcttcg
    34501 tccgcacggg gcagggcttc gggcggcagg aaggggtcgt tggcggcatt gagcaggagc
    34561 agcggtttgg caacgtgttt gagcagcggt ttgcaggaag tttggcggta gtagtcgtgc
    34621 cggtcggcaa agccgtgcag cggtgcggtg aaacggtcgt caaactcgcc cagtgttttg
    34681 caccctgcgg caaatgccgt ctgaaaacct tggagcgaac gtgctttggg tatcagtgtg
    34741 cggaggaagt agcgcgtgta gagcagccgc gtgatgccgc tgtcgaagcg gctgcctgcc
    34801 gcctctgcat caacgggggc ggatacggcg gccgaggcgt gcggcaatgc ctttttgccc
    34861 tgttcgccca aatattttgc cggcgcgttg ccgcccagcg atacgccgac ggcgtatatt
    34921 tcacggtaac gcgcggcgag cgtgtccaaa gcaaaggcga tttcggcggt atcacccaag
    34981 tggtagaaca ccggggcggt gttcgctacg ccgccgcagc tgcggaaatg gacgactgcg
    35041 ccgtgccaac cccgattgcg taccgcgagc atcagttcgg ccgcgtaatg gctgcggctg
    35101 cttccttcca aaccgtgaaa cagcacgacc agcggcgcat cgggcgaaat gccgcctgct
    35161 gaaaagtcgt aggcggtttt ggttttaccc gtgctgtcgg gaagcagctc gcggcggtat
    35221 gcgggtgcgg ggtgttgcag gaatttggcg gcaatcgtgt cggcattgcc gttgcggagg
    35281 aaaaagggcg tgtccggcgg tgtcaaaatc ataaggtatc ggtttttctg ttttcagacg
    35341 gcattggtga tgcggcagcc cgtccggccg gtgcggacgc gggcgatgcg cgcccggata
    35401 taggcgcgga aaaagcgttt tccgaaaaag gatatcggca tcggtcagct ttccacgcgt
    35461 ttgaaatggc gcggacggaa gcccaaagcc gccagagatg cgaaatacag tccgccgccg
    35521 acggcaatca ggatgcagag ctgccccgct ttccgcattc cgccggcgtg cgcccattcg
    35581 aacggcaggc aagcctgcgc cgcccacagt ccgccgcaca tcacggcgag cgcgagcagc
    35641 attttcgcca agaacgccgc ccaacccctg ccgggccggt aaataccgtg tttgcgcaac
    35701 aggaagaaca acaatccggc gttgatgcac gcgcccaggc cgatggcgag cgaaagcccg
    35761 gcgtgtttca acggaccgat aaaggcgagg ttcatcaact gcgtgcagat gagcgtgaag
    35821 atggcgattt tgacgggcgt tttgatgttt tgccgcgcat aaaagccgga tgccaacact
    35881 ttgatcataa ttaaaccgat taaaccgaaa gaataggcaa tcagcgcgtg ttgcgtcatt
    35941 tgtgcgtcaa acagcgtgaa ttctcggtac ataaacagcg tcgccaccag cgggaacgac
    36001 aataccgcca gtccggccgc cgccggcagc gtcagcagca tgcacaggcg caaaccccag
    36061 tcgagcaggg cggaaaactg ttccgtatct tggtttgccg agtgtttgga caaagtcggc
    36121 agcaaaattg taccgagtgc agcccccagc acgccgccgg gcagctccat catgcggtcg
    36181 gcgtaataca tccatgaaac gctgcccgat tgcagataag acgcgaaaat cgtgttgata
    36241 accaaagaaa tttgcgccac gctcacgccc aaaatcgcag gcgccatctg tttcatgacg
    36301 cggttgaccg ccgcatcttt gaaattcagt ttgggcagtt tcaaaaagcc cagtttcgcc
    36361 agccacggca gttggaaacc gagctgcaaa ataccgccga caaaaaccgc ccacgccagc
    36421 gcggtaacgg gcggatcgaa atacggcacg aaaaacagtg cgaatacgat aaaagagatg
    36481 tttaaaaacg tgggcgtaaa cgcgggaatg ccgaacttat ggtaggaatt gagtatcgat
    36541 ccgacaaaag aagacaaaga aatcaataat atataaggaa acgtaatccg cagcaggctg
    36601 atggaaagtt ggaacttgtc cgcgtctttg gtaaagccgg gcgcggaaac ataaatcacc
    36661 caaggcgcgg caagtatgcc cagcgcggta acgacgatca gcacaaacga cagcattccc
    36721 gcaacgtggc ggataaaagc ctccgtcgcc tctttagaac gcgtttcctt atattccgcc
    36781 aaaatcggca caaacgcttg ggcaaacgcc ccctccgcaa acacgcggcg aagcaggttg
    36841 ggcagtttga acgcgacaaa aaacgcatcc gtcgccatac ccgcgccgaa tgcccgcgca
    36901 atgaccgtat cgcgcacaaa tcccaaaacg cgcgacacca tcgtcaggct gccgactttt
    36961 gccaaagctc caagcatatt catcgtgttt ccttttcggc tgaaaccccg ccctttaggg
    37021 cggcag
//
LOCUS       DAXDSD010000006        35700 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXDSD010000006 DAXDSD010000000
VERSION     DAXDSD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35700
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.006"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(309..926)
                     /gene="thiE"
                     /locus_tag="ACFB2Q_000209"
     CDS             complement(309..926)
                     /gene="thiE"
                     /locus_tag="ACFB2Q_000209"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC0917331.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFQTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            complement(948..2048)
                     /locus_tag="ACFB2Q_000210"
     CDS             complement(948..2048)
                     /locus_tag="ACFB2Q_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC0917332.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIAAGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            complement(2045..3271)
                     /gene="cytX"
                     /locus_tag="ACFB2Q_000211"
     CDS             complement(2045..3271)
                     /gene="cytX"
                     /locus_tag="ACFB2Q_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC0917333.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVELSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKTQSLQRNPS"
     regulatory      complement(3337..3441)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3516..5000
                     /gene="tldD"
                     /locus_tag="ACFB2Q_000212"
     CDS             3516..5000
                     /gene="tldD"
                     /locus_tag="ACFB2Q_000212"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC0917334.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     gene            5144..5803
                     /locus_tag="ACFB2Q_000213"
     CDS             5144..5803
                     /locus_tag="ACFB2Q_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC0917335.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFGLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            5793..6461
                     /locus_tag="ACFB2Q_000214"
     CDS             5793..6461
                     /locus_tag="ACFB2Q_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC0917336.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKHQVESGLAPGLSRGQVFRCIELPQSIRRVLPGAVNLFTRMIKT
                     SSLAWLIGVIEVVKVGQQIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQ
                     KWEH"
     gene            6463..7194
                     /locus_tag="ACFB2Q_000215"
     CDS             6463..7194
                     /locus_tag="ACFB2Q_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC0917337.1"
                     /translation="MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            7307..8161
                     /locus_tag="ACFB2Q_000216"
     CDS             7307..8161
                     /locus_tag="ACFB2Q_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC0917338.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTAADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            8577..9398
                     /gene="prmC"
                     /locus_tag="ACFB2Q_000217"
     CDS             8577..9398
                     /gene="prmC"
                     /locus_tag="ACFB2Q_000217"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC0917339.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            9484..10872
                     /locus_tag="ACFB2Q_000218"
     CDS             9484..10872
                     /locus_tag="ACFB2Q_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC0917340.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            10873..11097
                     /locus_tag="ACFB2Q_000219"
     CDS             10873..11097
                     /locus_tag="ACFB2Q_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC0917341.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(11497..12390)
                     /locus_tag="ACFB2Q_000220"
     CDS             complement(11497..12390)
                     /locus_tag="ACFB2Q_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC0917342.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            12433..15135
                     /gene="ppc"
                     /locus_tag="ACFB2Q_000221"
     CDS             12433..15135
                     /gene="ppc"
                     /locus_tag="ACFB2Q_000221"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC0917343.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELGHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            15258..16247
                     /locus_tag="ACFB2Q_000222"
     CDS             15258..16247
                     /locus_tag="ACFB2Q_000222"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC0917344.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            16301..16630
                     /locus_tag="ACFB2Q_000223"
     CDS             16301..16630
                     /locus_tag="ACFB2Q_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917345.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            16641..16946
                     /locus_tag="ACFB2Q_000224"
     CDS             16641..16946
                     /locus_tag="ACFB2Q_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC0917346.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            16952..17131
                     /gene="ssrS"
                     /locus_tag="ACFB2Q_000225"
     ncRNA           16952..17131
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFB2Q_000225"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            17309..17740
                     /gene="rplM"
                     /locus_tag="ACFB2Q_000226"
     CDS             17309..17740
                     /gene="rplM"
                     /locus_tag="ACFB2Q_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC0917347.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            17753..18145
                     /gene="rpsI"
                     /locus_tag="ACFB2Q_000227"
     CDS             17753..18145
                     /gene="rpsI"
                     /locus_tag="ACFB2Q_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC0917348.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(18526..19020)
                     /locus_tag="ACFB2Q_000228"
     CDS             complement(18526..19020)
                     /locus_tag="ACFB2Q_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917349.1"
                     /translation="MRLTKYPYNSIRELEDYLINTYQKYIILQEGGKEIYRCFILSFY
                     KEFNIGIGLAVSCISIPPKVLMLDDKNIFIGFDSVVFCISIQNSKVNILNIDGIVFDI
                     YLLDNQKICIIHELGAIITDKNLIRENSVSTDIISDWEIDKVNKLIILKGLDSEKIIS
                     LNYD"
     gene            complement(19213..20142)
                     /locus_tag="ACFB2Q_000229"
     CDS             complement(19213..20142)
                     /locus_tag="ACFB2Q_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC0917350.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            20271..21020
                     /locus_tag="ACFB2Q_000230"
     CDS             20271..21020
                     /locus_tag="ACFB2Q_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC0917351.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     VHPILGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVAI
                     GNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            21143..21724
                     /gene="petA"
                     /locus_tag="ACFB2Q_000231"
     CDS             21143..21724
                     /gene="petA"
                     /locus_tag="ACFB2Q_000231"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC0917352.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            21743..23092
                     /locus_tag="ACFB2Q_000232"
     CDS             21743..23092
                     /locus_tag="ACFB2Q_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC0917353.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            23107..23895
                     /locus_tag="ACFB2Q_000233"
     CDS             23107..23895
                     /locus_tag="ACFB2Q_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC0917354.1"
                     /translation="MKQAMKNWFAALLSAVPMSAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDTYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLLAVMLPLAYFLKKEYWKDVH"
     gene            24227..26017
                     /gene="rmuC"
                     /locus_tag="ACFB2Q_000234"
     CDS             24227..26017
                     /gene="rmuC"
                     /locus_tag="ACFB2Q_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC0917355.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIDLQEKAQGLAVENERLATQLGQERKAFAEQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(26084..26716)
                     /locus_tag="ACFB2Q_000235"
     CDS             complement(26084..26716)
                     /locus_tag="ACFB2Q_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC0917356.1"
                     /translation="MTLRYEILPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLETHKVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            26809..27633
                     /locus_tag="ACFB2Q_000236"
     CDS             26809..27633
                     /locus_tag="ACFB2Q_000236"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC0917357.1"
                     /translation="MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            27702..28265
                     /locus_tag="ACFB2Q_000237"
     CDS             27702..28265
                     /locus_tag="ACFB2Q_000237"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC0917358.1"
                     /translation="MTDLETKRLETQAMLENAELLFDQGQCRAALQKVAYEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKEKPVKADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALGGK"
     gene            28349..28786
                     /locus_tag="ACFB2Q_000238"
     CDS             28349..28786
                     /locus_tag="ACFB2Q_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC0917359.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDFAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            29007..29276
                     /locus_tag="ACFB2Q_000239"
     CDS             29007..29276
                     /locus_tag="ACFB2Q_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC0917360.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            29276..31051
                     /gene="ptsP"
                     /locus_tag="ACFB2Q_000240"
     CDS             29276..31051
                     /gene="ptsP"
                     /locus_tag="ACFB2Q_000240"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC0917361.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(31178..32119)
                     /locus_tag="ACFB2Q_000241"
     CDS             complement(31178..32119)
                     /locus_tag="ACFB2Q_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC0917362.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(32128..33003)
                     /locus_tag="ACFB2Q_000242"
     CDS             complement(32128..33003)
                     /locus_tag="ACFB2Q_000242"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC0917363.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(33186..33262)
                     /locus_tag="ACFB2Q_000243"
     tRNA            complement(33186..33262)
                     /locus_tag="ACFB2Q_000243"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33226..33228),aa:Met,seq:cat)
     regulatory      33411..33510
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33601..35502
                     /gene="thiC"
                     /locus_tag="ACFB2Q_000244"
     CDS             33601..35502
                     /gene="thiC"
                     /locus_tag="ACFB2Q_000244"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC0917364.1"
                     /translation="MTTPKKTAKISGNEARELSDLSEDIGIRFKYQNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIECAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLHYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         8429 a         9657 c         9847 g         7767 t
ORIGIN      
        1 ttccgtacta tttgtactgt ctgcggcttc gccgccttgt cctgattttt gttaatccac
       61 tatatctagc cgaattactt tattttttga tacgcaaccg gccggttgcc gtcattcccg
      121 cgcaggcggg aatccatttt tgaaattcgg aaactgtttt tcaaatgatg gtttctcaag
      181 ttttacggcg gattcccgcc tgagcgggaa tgacggaatt tgatgatatg ccacatttaa
      241 agttaagcat atttgcgata acgggctaag ttttgcctac acggcaattg aattttcttc
      301 tttcggtttt atccatccca caaagcctga aacgctttaa ccaccgcttc gggatttgcc
      361 gcttcggtta cggcgcggac ggcggcgagt gaggaaactc cggtggccag cacggctcgg
      421 gcgttgttca aatcgatgcc gccgatggcg acgacgggcg tgccgcgagc ctgttccaca
      481 tattcgcgca atttgtccaa gccttgcggg gcggtgggca tttgtttggt cgtggtctgg
      541 aaaatcgcgc cgctggcgat gtagccgggg tgtacggaca gggcgcggtc gagttcggcg
      601 acggagtgcg tactcaaacc caaacgcaaa ccggcggcgg cgatggcggc aaggtcggcg
      661 gtgtccatgt cttcctgccc gagatgtacg ccgtatgcgc ccgcttcgat tgcctcgcgc
      721 cagtggtcgt tgatgaaaag ctgtgtgcgg ctgccttgac aggcggcgac gcagcgggcg
      781 atttcgcgtt tcaattcgtt gccgtgcaag gttttacagc gcagttgcac cgtgtcggca
      841 cctgctttga ccatgcgccc cacccaatcg gcggtgggaa cgacggcgta gaatttaagt
      901 agggatttta ggggcgggaa ggtcataggg tgtcctttcg ggcggcttta atcttgtctt
      961 ccgatatacg ccaaaccgct ttcttcatca cgttcgggcg cgtcttttcc gtcaaacagt
     1021 gccactgcca atctgacggc ggcggcggtt acggcggggg aaatcataaa gccgtgccgg
     1081 aaaaggccgt tgatttcgat gaggcggcgt tcgcggctgt agcggatttc ggggttgtgg
     1141 tggttgagcg tggggcgcag gccggcggcg atttcgagga tgtcggcttc gccgaaggcg
     1201 gggtggacgg catagagcgc ggataagagt tccagcccgg aacgtacgct ggcgggggct
     1261 tggctttcgc tttcgatttg ggtcgcgccg atgacgaaga cgtggttttc tttcggggcg
     1321 atgtagagcg gatagcgcgg gtgcagcagg cgcacggggc ggttgagcgt gatttcgggc
     1381 gtgtaaaccc gcgccacttc gccgcgtatg ccgcgcaagg tgctggtgtg ctcgggggat
     1441 tggttccacg cggttttcgc gccgtagccc cggcagtcga ttacccagtc gtattgggct
     1501 tgcaggtctt ggggggcgca ttcgtgttcc caatggcaag ggacgttcag ttcgtccaaa
     1561 gcgtcggcaa gtgcagacaa tatttgccgc ccgtcgagct ggccttcggt cggcaggtag
     1621 atgccgtctg aaaaacgtcc gccgagttgc ggttcgcgtt cggcgatttc atcggcgcgc
     1681 caacggacga tttcgtcatc cgctacgccg ccgcgtttga gatggcggac gaactcgctg
     1741 gataatggct tgtcctgccc gtgccacaca atcaggctgc cgttttcctg catcatcgtg
     1801 tgcgtgttca gacggcatcg gatgccgcgc caaagcggaa tgctctgcct gcccagcctg
     1861 atgacttcgg gcgttgcctc gaccgcttcc gccgcaggcg cgagcatcgc ggcggcaaca
     1921 taggcggcgg cgtgttcgcc ttggcgggtg cccttgtcga aaagttcaat ctgataacct
     1981 tgttctgcaa gctgcaatgc ggtcagcctt ccggaaaggc cgcctccgag gacggcgata
     2041 cgggtcatga cgggttcctt tgtaaagatt gggttttttt aaagaaaagg cgtaccgata
     2101 cggtggcaat ggcaacggca gacattacgg gggcggtcag accgatgctg ctttcccaac
     2161 cggacgagag caggaagcgg tagaggatga agcctgccag ccacagaacc agtccggcaa
     2221 agtcaaagcc ttcaatctcc tcacgccgtt ttaagacgaa aaagtcggca atcaaaaccg
     2281 ccgccatcgg cgcaaatacc gagccgataa gcagcaggaa gttttcatat tcggtaacgg
     2341 gcagcatgac ggcaagcacc gtgccgatca gggtaacgcc gacagcgacg ggtgtttccg
     2401 caaaacgcgc ggaaatgttg ttcgcactcg cgccggcgga ataggtatcg agaaacgttg
     2461 tggtaacggt ggagaggacg actgccagaa tgcccgttat gcccaagccc gcgcccaaca
     2521 ggattttcgc cacgtcggtt tctccggtaa acagagccgc cgccaaaccc aaggcataca
     2581 tccagcagcc cgtcagcgta taggcgagcg ttgccgtcag ggttgccgca aacgggcggc
     2641 gtgcttggcg cgtgtagtcg gcggccagcg gcagccagga aagcggcatg acggcggaca
     2701 gttcgactgc cgttccgaag gtcatgccgt ctgaaacggc gggcgcggcg tttgtgccgg
     2761 acgaagcgaa cacttcgacg ctcaaccaca acacggcaag cagcatcagc agcatcgaaa
     2821 cggttttcag cccgcccgtt ctgcgtgcgc cgaaaaccag ccacagcacg atcagtgcgc
     2881 cgtttgccaa tgcccaccag acaaaggatt cgccgtccca caacactttg cccaaagcgg
     2941 agctgaccgt tgcgccgacg taaatcatca ccgccgtcca gccggccagt tgcagcatat
     3001 tcgccacgga aaacagcact gaaccgcatt tgccgaacga caggcgcaca ctttccatcg
     3061 agctgcgtcc ggtcagtgcg ccgatatacg ctgccgcaaa aaacagcgcg ccgccgacgg
     3121 catgacccaa aagcagggcc gccagaccgc gctgccagcc caagggggcg agcagcgtac
     3181 ccgtgctgat ttcggcaatc gataccgccg cgccgaacca aatcagcccg atggcggcgg
     3241 aagatgaagg agaggaggca ttgcccgaca tatgacgtcc tttaaaaaga aggaaggtgg
     3301 gcggggaagt gcggaaaagg gaacggaagc ccgaaagggc ggggaggttt tgtttcctac
     3361 gcaggcatta ccccgacagg ttctacggat tttgctttcg caatctcagc cgcccggcgg
     3421 gcggcactcc gacaagaagg ctttaagttt acacaaagca acgtgcgttg gcaaaattct
     3481 tacattttcg acggcacggc gcatttccat aacaaatgcc gtataatcaa gcccctgatt
     3541 ttttatgcgc cgacactatg catccgacct attccgccgt acaggcgcgg ctgctcgaag
     3601 ccaaccgcct ttcccccgaa ctgctcgcca aaagcctgtg catcatcggt gcgcaccacg
     3661 tcgattatgc cgatatctac tgccagcgca ccgcttatga aagctggcat ttggaagagg
     3721 gcatcgtcaa atcgggcagc ttccaaatcg atcagggtgt gggcgtgcgc gccgtttcgg
     3781 gcgacaaaac cgcctttgcc tacgccgaca gcctgtgcat cgattcgata aaccgttccg
     3841 cccgcgccgt ccgcgcgatt ggggcggcag gcggcaaggt gtccgccaaa atgccgtctg
     3901 aaacgcgcgg caagccggtt tgttccgcat ccgaccccat tgccggcctc gattccgccg
     3961 ccaaagtcgc gctgttgaac aaagtggaag caatcgccaa agccgccgat ccgcgcatcg
     4021 tgcaagtgat ggccggtttg acctgcgaat acgatatggt ttacctcgcc cgtctggacg
     4081 gcaaacacgc cgccgacatc cgcccgatgg tgcgcctgaa cgttaccgtc atcgccaaac
     4141 agggcgaacg ccgcgaacag ggcggcgcgg gcggcggagg acgctacgac ttggcttatt
     4201 tcgatgaaac cttggtgcgg cagtttgtcg atgccgccgt caaacaagcc ctgaccaatc
     4261 tcgaatcccg tcctgcgcct gccggcgaga tgaccgtcgt tttgggcaac ggttggccgg
     4321 gcgtgttgct gcacgaagcg gtcggacacg gtttggaagg cgatttcaac cgcaagggga
     4381 ccagcgtctt ttccggccgg atcggcgaac gcgtcgccgc caaaggcgtt accgtcgtcg
     4441 atcagggcga tattgccggc cggcgcggtt cgctcaacat cgacgacgaa ggcaacgaaa
     4501 cacgccgcac cgtattgatt gaagacggca tcttggtcgg ctatatgcag gacgaaacca
     4561 acgcccgcct gacgggtacg caatcgaccg gcaacggtcg ccgccaaagc tacgcctccg
     4621 tcccgatgcc gcgtatgacc aacaccttta tggaaaacgg cagctacgag ccggaagaaa
     4681 tcatcgcgtc catcgacaag ggcatttacg ccgtcaactt cggcggcgga caggtggaca
     4741 tcaccagcgg gaaatttgtc ttcggcgcgt ccgaagcgtg gtgggtggaa ggcggcaggc
     4801 tgcaataccc cgtcaaaggc gcgaccatta tcggcaacgg ccccgaagtg ctgaaacacg
     4861 tttcgatgat aggcaacgat accgcgctcg acagcggtgt cggcgtgtgc ggcaaagaag
     4921 ggcagagcgt ccccgtcggc gtggggcagc cgaccctgcg tatcgatgcc gggctgaccg
     4981 tcggcggcag cgcaatctga cgcggaatcc ggcacgatgc cgtctgaaag gttttcagac
     5041 ggcatcgcct tattcttttt tccgatgttt gatattccta agcattcttt aacattatct
     5101 tacaagttca ttaaaatacc gcctttcgtt aagctgattt ctgatgaact ggccttattt
     5161 aatcgatgcc gtacccaaat tcgccgatgc ggcaaagctg acgctggaat tgtccgttta
     5221 cggcgttgtt ttgtcgttgc tgttcggcct gcccgtcgcc gtggtaacgg cataccgcat
     5281 ccgccccttc tacgcattgg cgcgcgccta tatcgagctg tcgcgcaata cgcccctgct
     5341 gatccaattg tttttcctat attacggtct gccgaaaatg ggcatcaaat gggacggttt
     5401 cacctgcggc gtaatcgcgc tggttttctt gggcgcaagc tatatggcgg aagctgtccg
     5461 cgccggcatc ctcgccgtcc ccaaagggca ggtcggagcg ggcaaggcaa tcggtttgag
     5521 ccgctttcaa gtgttccgct atgtcgaatt gccgcaggtt tgggcggtcg ccgttcccgc
     5581 catcggcgca aatatattgt ttttaatgaa agaaacatcc gtcgtcagca cggtcggcat
     5641 tgcggaattg ttatttgtta ccaaagacgt catcggtatg gactacaaaa ccaatgaagc
     5701 cttgttcctg ctgtttgccg cttatttgat tatcctgctg cctgtttctt tgttggcgcg
     5761 gcggattgaa aaccgcgtac ggagtgcgaa atatggcgtt tgaatggctg tttgaaggaa
     5821 aaaatgcggc acggttgggc gaaggcctgc tcttaacggc acaaatttct ttaatctctg
     5881 ttgcggcttc ttgcgtattg ggcacgctgt tcggcttggt tttgcgttcg cgcaaccggc
     5941 ttgtccgctt tgtcggacgg ttttatctcg aaaccatccg aatcgtgccg attttggtgt
     6001 ggctgttcgg cctgtatttc ggcctatccg tctggacagg catccatatc ggcggatttt
     6061 gggtctgcgt ctgggtattt tccctgtggg gcgttgccga aatgggcgac ttggtgcgcg
     6121 gcgcactgga atcgattgaa aaacaccagg tcgaatcggg tcttgcgccc ggcttgagcc
     6181 gggggcaggt attccgctgc atcgagctgc cgcaaagcat ccgccgcgta ttgcccggcg
     6241 cggtcaacct gttcacgcgc atgatcaaaa ccagctcgct cgcctggctt atcggcgtga
     6301 tcgaagtcgt caaagtcggg cagcaaatta ttgaaaactc gttgctgacg cagcccaatg
     6361 cttcattttg ggtttacggc ctgattttta tgctgtattt cttctgttgc tggccgctat
     6421 ctctactggc ggcaaaactt gaacaaaaat gggaacactg acatggcttt actgagcatc
     6481 cgcaagctgc acaaacaata cggcagcgta accgccatcc aatccttaga cttggacttg
     6541 gaaaaaggcg aagtcatcgt actgctgggc ccgtccggct gcggcaaatc caccctcctg
     6601 cgctgcgtca acggtttgga gccgcaccaa ggcggcagca tcgtgatgga cggtgtcggc
     6661 gaattcggca aagacgtttc ctggcaaacc gcccggcaaa aagtcggtat ggtctttcaa
     6721 agctacgaac tgtttgccca catgaccgtc atcgaaaaca tcctcttagg cccggtaaag
     6781 gtacaaaacc gcgaccgtgc cgaagcagag gcgcaagccg gcaaactgtt ggaacgcgtc
     6841 ggactgctag accgcaaaaa cgcctatccg cgcgaacttt ccggcggtca gaaacagcgc
     6901 atcgccattg tccgcgccct gtgcctgaat ccggaagtca tcctgctgga cgaaatcacc
     6961 gccgcacttg accccgaaat ggtgcgcgaa gtcttggaag tggttttgga actcgcccgc
     7021 gaagggatga gtatgctcat cgtaacccac gaaatggggt tcgcacgcaa agttgccgac
     7081 cgcatcgtct ttatggacaa aggcggcatc gtcgaatcgt ccgaccccga aacctttttt
     7141 tccgcaccaa aaagcgaacg cgcccgccaa tttctggcag gtatggacta ctgatttacc
     7201 gcaaaccgtc cgaacccttt tcagacggca tttcataggg cagtgcttta tgtgtcgtcc
     7261 tgtgtgttga aacatcccaa gcaaacattc aaaaaggaga tttccaatga aattgaacgc
     7321 caaactcaaa gcccttttgg cttccgccgc catcgccgtc ggtctgaccg cctgcggggg
     7381 cggctccggc gatgcccaat cttcacaaag cagcggtgcg gcaaccgttg ccgccatcaa
     7441 agaaaaaggc gttatccgca tcggcgtatt cggcgacaaa cccccgttcg gctatgttga
     7501 cgcaaacggc aaaaaccaag gctttgacgt tgaaatcgcc aaagacctgg ccaaagacct
     7561 gctcggcagc cccgacaaag tcgaattcgt cctgaccgag gccgcaaacc gcgtcgaata
     7621 cgtccgttcc ggcaaagtcg acctcatcct cgccaacttt acccaaaccc ccgaacgcgc
     7681 cgaagccgtc gatttcgccg atccttacat gaaagtggcg ttgggcgtgg tttcccccaa
     7741 aaacaaaccg attaccgaca tggcgcaatt gaaagaccaa accctgctgg tcaacaaagg
     7801 caccgccgcc gacgctttct tcaccaaaag ccatcccgaa gtcaagctgt tgaaattcga
     7861 ccaaaacact gaaaccttcg acgcgctgaa agacggtcgc ggcgtagcac tcgcgcacga
     7921 caacgccctg ctgtgggcgt gggcgaaaga aaacccgaac tttgaagtcg ccatcggcaa
     7981 tctcggtcct gccgaattta tcgcccccgc cgttcaaaaa ggcaacgccg acctcctgaa
     8041 ctgggtcaac ggcgaaatcg ccgccatgaa aaaagacggc cgtctgaaag ccgcctatga
     8101 aaaaaccctc ttgcccgtgt atggcgagaa agtcaaaccg gaagcattgt tggccgaata
     8161 agtttgtttg acaagaaaat ccgtatccgc atttgaaggc gggtgcggat ttttttcagc
     8221 cgccgaaaat attgcaaatt caaaggaaaa atcaactatc tcatcgtttt atttatttta
     8281 ttattcttgc gcgtgcggaa atcaagttcg tccggttttg cctgtttttg aatttcggga
     8341 aacttccaaa tcgtcattcc agataaaacg cttcagcctt atgtttccgg atgaccgttt
     8401 tcgcgggatg acggcggagc gtttctgtta ttcggataaa ttcctacggt attgaaacgg
     8461 cgataaggtg ttttccttta ttatataatt tcaatgcaaa atgatatttt ttgaacaagc
     8521 gtgccgcaaa gccggaaaaa atgttcaagc cgcatcggcg gcagacggga cgaaaaatga
     8581 cgtttgacga atggttgggc ttgtcaaaac tgcctaaaat tgaagcaagg atgctgctgc
     8641 aatatgtttc ggaatatacg cgcgtgcagt tgttgacgcg gggcggggaa gaaatgccgg
     8701 acgaaatccg acagcgggcg gacaggctgg cgcaacgccg tctgaacggc gagccggttg
     8761 cctatatttt aggtgtgcgc gaattttatg gcagacgctt tacagtcaac ccgaacgtgc
     8821 tgattccgcg ccccgaaacc gaacacttgg tcgaagccgt attggcgcgc ctgcccgaaa
     8881 acgggcgcgt gtgggatttg gggacgggca gcggcgcagt tgccgtaacc gtcgcgctcg
     8941 aacgccccga tgcgtttgtg cgcgcatccg acatcagcac gcccgccctt gaaacggcgc
     9001 ggaaaaatgc ggcggatttg ggcgcgcggg tcgaatttgc acacggttcg tggttcgaca
     9061 ccgatatgcc gtctgaaagg caatgggaca tcatcgtgtc caacccgccc tatatcgaaa
     9121 acggcgataa acatttgtcg caaggcgatt tgcggtttga gccgcaaatc gcgctgaccg
     9181 acttttcaga cggcctaagc tgcatccgca ccttggcgca aggcgcgccc gaccgtttgg
     9241 cggaaggcgg ttttttgttg ttggaacacg gtttcgatca gggcgcggcg gtgcgcggcg
     9301 tgttggcgga gaatggtttt tcgggagtgg aaatcctgcc ggatttggcc ggtttggaca
     9361 gggttacgct ggggaagtat atgaagcatt tgaaataatt gtttgcaaaa ttggcggatt
     9421 ttccgttatt gggttatgat gtgttttgat atgtaaacat ttataggttt ggagcgataa
     9481 acaatgaatg cagtcgtagt tgccgtaatc gttatgctgg tgctgtcgct gtcgcgcgtg
     9541 cacgtggtat tgagcctgac ggtcggcgcg tttgtcggcg gcgcggtggc gggtatgccg
     9601 ctgcaaaaca ttgccgatgc ggcgggacag gtcagtcagg cggggattat ccccgtgttc
     9661 aacaaaggtt tggaaggcgg cgcgaagatt gcgctttctt atgcgatgct tggtgcgttt
     9721 gcgatggcga ttacccattc cggcctgccg cagcagcttg ccggcgcggt cgtccgcaag
     9781 ctgaaccggg gcggtatgcc cgacagcgtg cgttcgggcg agggcgcggt caaatggctg
     9841 ctgctttcca tcatccttgt gatgggcatt atgagtcaga acgtcatacc catccacatc
     9901 gcctttatcc cgatgattgt tccgccgctg cttttggtgt ttaaccgcct gaaaatcgac
     9961 cgccgcctga ttgcgtgcgt catcactttc gggctggtta cgacttatat gttcctgcct
    10021 tacggcttcg gcgcgatttt tttgaacgaa atcctgttgg gcaacatcca ttccgccgcg
    10081 ccgcagcttg atgtgaaaaa catcaacgtg atggcggcaa tggcgattcc cgcattggga
    10141 atgctggccg gactcctgct ggcgtttgtc cactaccgca aaccgcgact gtaccaaagc
    10201 aacaatgccg atacggcggg caacgccgat gcggcaaacc gtccgcagcc gtccgcctac
    10261 cgcagcctgg ttgccgccgc cgccatcgcc gtatgctttg ccatacagtt gatgtatgaa
    10321 gattcgctgg tgttgggcgc gatgctcggt ttcgccgtat ttatgatgtt gggggtcatt
    10381 aaccgcgaca aggcaaacga cgtattcggc gaaggcatca agatgatggc gatggtcggc
    10441 ttcattatga ttgccgcgca gggttttgcc gccgtgatga atgcgaccgg gcatattcag
    10501 ccgctggtgg aaagcagtat ggcgatattc ggcaacagca aaggtatggc ggcgttggcg
    10561 atgctggtgg tggggctttt ggtaacgatg ggcatcggtt cgtccttttc cactttgccg
    10621 attattgccg cgatttatgt gccgctgtgt accggtttgg gcttttcgcc gcttgccacc
    10681 gccgccattg tcggcacggc gggggcgttg ggcgatgccg gttcgcccgc gtccgattcc
    10741 acgctgggcc cgacgatggg gctgaacgcc gacgggcggc acgaccatat ccgcgattcc
    10801 gtcatcccga ccttcatcca ctacaacatc ccgcttctga ttgccggctg gattgccgcg
    10861 atggtgctgt aaatggacgc ggttcaagag ttggaacgcc gtattgtcga actggaaatc
    10921 caaaccgcgc ttcaggagga cgtaatctcc ggtctgaacg cgatggtggc ggaattgcgg
    10981 cagacgttgg atttgcagca ggctcagttg aggctgctgt atcaaaaaat gcaggacagg
    11041 aatcccgacg cgcaagagcc gtattccctg cgcgacgaga ttccgccgca ttattgatgc
    11101 gccgccgtat ccggatttcc ttaaataaag gctgtgtttg aatattccgc cggtgcaacc
    11161 ctaagatgta accttaatca gtcggacaaa atgcctttta tccgatgaaa ccggttcttg
    11221 tccgaacggc gatgagcggc ttcccccgct gcaaacagat aaaaccaacc gggtattcaa
    11281 acacagccaa aaaaagccgt ccgaacccaa aggggacaga cggccaaaca cattacgggg
    11341 aaaacgtctt actcaatgag tctgcgaaac agacattgct aaaacaactg caattataag
    11401 gtaagtttat ttttttgcaa agaaatgtaa atttttaaat ttttaaaacc gttccgccgc
    11461 atccgttcgg atgccgtctg aaacggcggc agggttttac cgtgccgcgc cgcaaaccgg
    11521 gcattcgggg ttgcgcggca ggtcgaaata ttgccagccc ccttccaagg cacggtaaac
    11581 cgccagcctg ccgtgcgacg gttcgcccgc ttccagcagg attttcagag cctccgccgc
    11641 ctgtgtgcag ccgacgatgc cgaccagcgg cgagagcacg ccgaagaggg aacagatgcc
    11701 gtctgaagcc gatccgccgt cgaacaggca ggcgtaacac ggcgagtcgg gcaagtcggg
    11761 acggtatgcg gcaagctgtc cttcaaaacg taccgccgcc cctgaaacca gcggtgtttt
    11821 cgtttgcacg caggcacggt tgaccgcctg ccgcgtggcg tagttgtcgc agcagtctaa
    11881 aacgatgtcg gcggtttgaa ccaaaccggt caggcggcag ccgtcgagtt tttcgttgac
    11941 ggcgcggaca tcgacagtat ggttgatgcg tttcaggcgg cctgccaagg tttcggcttt
    12001 aggcttgccg acatcgccct cgtcaaatgc gacttggcgt tgcaggttgt gcagctcgac
    12061 cgtgtcggaa tcggctatgg tcagcgtgcc gacgcccgag gcggcgagat agggcagggc
    12121 ggcggcgccc aatccgccgc agccgacgac caaaatatgc gcggcggaaa gtttctgctg
    12181 cccttcgatg ccgatttcgt ccaagaggat gtggcggctg taccgcagca ggagtgcgtc
    12241 gtcgttgtcg tgttcggtcg cggtcatgat gatgttcgga aaaaacagtt gcgggcgatt
    12301 gtaacgccgc cgtcgggcgg cgttcaactt cagacggcat ttcgggacat gagtggttaa
    12361 agtgtgaacg gtttggcacg gatgcggcat ttgaggtaca tttacagtat ttacggcaat
    12421 gagaggaaaa tcatgcaact gcatatcctg aacaatccga aggacgcggc tttggcggcg
    12481 gacgcggaat ttttgaaaca gtccctgttc aacctcctgc acgaagaagc ctcgccgttg
    12541 gttgtcgaaa cggtcaaact cttgtccact tccgacgaca gcgcggcatt gattgaaaaa
    12601 gtattgccgc aattggacga acggcagacc tacgatctga cgcttgcctg cgggctgttc
    12661 gcccagattc tgaatatcgc cgaagacgtg caccacgaac gccgccgcca aatccacgaa
    12721 gatgccggcc acaacgcagc agaaggcagc ctgacggaaa ccgtccgcag gctcaaagcg
    12781 gggaaagccg acggcaaatc ggtgcagcgg cagttggaca atacgtccgt taccgccgtt
    12841 ttgaccgcgc acccgaccga agtgcaacgc caaaccgttt tgaatttcaa ccgccgcatc
    12901 cgcgcgctgc tgccgcaacg cgaacgctgc accaatgccg acgcgctggc acggctgcgc
    12961 cgcgaaatcg acaccgtgct tctgggcttg tggcagacca gcgaaacgcg ccgccacaaa
    13021 ctcagcgtca acgacgaaat caacaacggc gtgtccatct tcccgatgag cttttttgaa
    13081 gccctgccca aactctaccg caagatggaa cacgactttc agacggccta tcccgatgtc
    13141 cgcgttccga acatccttaa aatcggcggc tggatcggcg gcgaccgcga cggcaatccg
    13201 tttgtttctg gcgaaaccct gcgctttgcc ttccgccgcc acgccgatgc cgtgttccgt
    13261 ttttaccgca gcgaactcga caaactttac cgcgaactgc cgctttccat ccgccgcgtc
    13321 aaagtcaacg acgacgtaat ggcactggca gccctctcgc ctgacgaaga aatcgcccgc
    13381 accgaagaac cttaccgccg cgccattgcc tacattatgg cgcgcgctat gggcaaagcg
    13441 cgctcgctcg gtttgggcat gggctgcaaa ttcggctttc tcgagcctta tgcttcggcc
    13501 caaaaatttc tcgatgattt gaaaaaattg caacgttccc ttatcgacaa cggcagccgc
    13561 ctgcttgccg aaggccgttt ggcagacctc atccgttccg tgtccgtgtt cggcttccac
    13621 atgatgccgc tcgacctgcg ccaacacgca ggcaaacacg ccgatgtggt tgccgagctt
    13681 ttccaacacg caggcttgga agactacaac agcctgaacg aagagcaaaa acaagccgcc
    13741 ttgttgcgcg aattgggcca ccaacgtccg ctgtacagcc cgttcatcac atacagcgac
    13801 catacccgcc gcgaattggc gattttcaac gaagcgcgca aaatcaaaga cgaatttggc
    13861 gaagatgccg taacacaaag cattatttcc aactgcgaac aacccggcga cctgctcgcc
    13921 ttggcattgc tgctgaaaga aagcggcctg ttggcggtgg aaaacggcaa accgcacagc
    13981 cgcatcaaca tcgtgcctct gtttgaaacc atcgaagcgc tcgaaaatgc ctgtccggtc
    14041 atggaaacca tgttccgcct cgactggtat gatgccctgc tcgaaagccg cggcaacatc
    14101 caagaaatca tgctcggcta ttccgactcc aacaaagacg gcggctacgt taccagctca
    14161 tggtgtctgc atcaggcgga attgggcttg gtcgaactct tcaaaaaata cgatgtccgc
    14221 atgcgcttgt tccacggtcg aggcggcagc gtcggacgcg gcggcggccc gtcttaccaa
    14281 gccatcttgg cgcaacccgc aggcagcgtt gccggacaaa tccgcattac cgagcaaggc
    14341 gaagtcatca ccgccaaata cgccgacccc ggcaacgccc aacgcaactt ggaaaccttg
    14401 gttgccgcga ctttggaagc cagcatcctg ccggataaaa aagaccctga tgccaaactg
    14461 atgcaggcat tgtcggacgt atcgttcaaa tactaccgcg aactgattac ccaccccgac
    14521 ttcatcgact acttcctgca aaccagcccg attcaggaaa tcgccaccct caacctcggc
    14581 agccgtcccg ccagccgcaa aaccctggcg cgaattcagg atttacgcgc gattccgtgg
    14641 gtattctcct ggatgcaaaa ccgcctcatg ctgccggctt ggtacggttt cggcagcgcg
    14701 gtggaaacct tgtgcgaagg cagccccgaa accctcgccg ccctgcgcgg acacgcccaa
    14761 aacaacccct tcttccaagc gatgctctcc aatatggaac aagtgatggc gaaaaccgac
    14821 atcaccttgg cggaaaacta cgccggtttg agcgaatcac ccgaaaaggc aaaagtcatc
    14881 ttcggcatga ttaaggaaga ataccgacgc agccgcaaag ccctgctcga cctgctgcaa
    14941 accgaagaac ttttgcgcga caaccgcagc ctcgcccgtt cgctcgcttt gaggattcca
    15001 tacctgaacg cgctcaacgg tttgcaagtc gccatgctca aacgcctgcg caaagaaccc
    15061 gacaatccgc acgccctgct gatggtgcac ctgaccatca acggcgtggc gcaaggtttg
    15121 cgcaataccg gctgatagtg ccgcatcggg gcaaaatgcc gtctgaacgc ctttcagacg
    15181 gcatttacct gaccgcactt gcagagaaac accgattgtt ttaaagtgaa cggcagtgat
    15241 atgttgaaag acgaccaatg aaaattaccg ttatcggcgc aggttcgtgg ggtacggcgc
    15301 tcgccctgca tttttcccaa cacggcaacc gcgtatccct gtggacgcgc aacgcagacc
    15361 aagtccgcca aatgcaggaa gcgcgtgaaa acaaacgcgg actgcccggc ttttcctttc
    15421 ccgaaacctt ggaagtgtgt gcggatttgg cagaggcgct caaagacagc ggacttgtcc
    15481 ttatcgtaac ctccgttgcc ggattgagaa gcagcgcaga gctgctcaaa cagtacggcg
    15541 cgggacacct ccccgtcctc gccgcctgca aaggcttcga gcaggataca gggctgctga
    15601 ccttccaagt cttgaaagaa gtattgcccg acaataagaa aatcggcgta ctttccggtc
    15661 cgagttttgc acaggaactc gccaaacaac tgccctgcgc cgtcgtcctc gcctccgaaa
    15721 accaagagtg gattgaagaa ctcgtaccgc agctcaacac gaccgtcatg aggctttacg
    15781 gcagtaccga cgttatcggc gtggcagtag gcggttcagt caaaaacgtc atggcaatcg
    15841 ctaccggact ctccgacgga ctcgaatacg gactcaacgc ccgtgccgca ctggttacgc
    15901 gcggactggc cgaaatcacc cgccttgcct ccgcaatggg cgcgcagccc aaaaccatga
    15961 tggggcttgc cggcatcggc gatctcatct tgacctgtac cggtgcactt tcgcgcaacc
    16021 gccgcgtcgg cttgggtttg gcagaaggca aggaactgca tcaggtgctg gtcgaaatcg
    16081 ggcacgtttc cgaaggggtc agcaccatag aagaagtctt caataccgcc tgcaaatacc
    16141 aaatcgatat gccgattacc caaaccctgc tgcaactcat ccgcaaagaa atgacccctc
    16201 agcaggtcgt cgaaaggctg atggaacgca gcgcgcgttt cgaataaaca acaaacagat
    16261 gccgtctgaa cccccagacg gcatacagac aggtaaggtc atgaaacaaa atatcgaaaa
    16321 actcgaaagc agcgtttata cgttggtaca aaaattcgaa accctcgtca gcgaaaaccg
    16381 ccgcctcaaa gaaaccgtcg ccgaactcga acgggcgcac gagcggcaaa aactcgaaca
    16441 cgagaccgcc gtcgacgaac tcagcgaagc cctgctcgtc caagtcggca aactcaaaga
    16501 agacctgcaa aacaaaatcg acagcctgac ggaagaaaat gcacgatacc gcgccctgct
    16561 cgaacagagc agggagaaaa tcagcgcact ggcagcgcgc ctcccccaac ggcaggaaac
    16621 gcagcaataa ggattaaagg atgaacatcg aacaagtcta catcgaagtc atgcacgccc
    16681 ggctgaccgt caacacgccg gcagaagaaa aagacacact gttgcaggca gtcggaatgc
    16741 tcaacggcaa agccgaagcc atccgcgaag gcggccgcgt cgcggacagc gaaaaaatcg
    16801 tcattatggc cgcgctcaac gtcgtccacg acctattgaa aacctccctg aacggcggcg
    16861 atttggcaat cggcgatttt gcgcgtaaaa taaccgatat ggacaacgcc tgccaaaagg
    16921 cactatcccg cttggggcag gaataacccc tttttccctg cggtgtccgc gaaggcatat
    16981 attcctttga accaataagt tcgcttaagg ttgccgggag cagtagtggg cgcgagcgtc
    17041 cttttgcgga cgcacccgaa actacccggg gcagccgcct tgtcagcaag gttcaagcgg
    17101 attcggccga aacggctctc gcggggattc ccattcaaaa ccgcatcgca acgatgcggt
    17161 ttttcacatt ggccgccgcc ccgcccgaaa aagcagtatg cagtctgccg aaatccggaa
    17221 aatccttccg taacgcggta atgcgattga caaaatcaaa tcctgtcttt aaaatccata
    17281 actttctata tctatctgga tttccactat gaaaaccttt tcagcgaaac cccacgaggt
    17341 gaagcgcgaa tggttcgtca tcgatgccca agacaaagtc ttgggtcgcg ttgcaaccga
    17401 agtcgccagc cgtctgcgtg gcaaacacaa acctgaatac accccccacg tcgataccgg
    17461 cgattacatc atcgtcatca atgcggacaa actgcgtgta accggtgcca aattcgaaga
    17521 taaaaaatac ttccgccatt ccggttttcc aggcggcatc tacgagcgca ctttccgcga
    17581 aatgcaagat caattcccgg gccgcgcttt ggagcaggct gtaaaaggta tgctgcccaa
    17641 aggtccgctg ggttacgcca tgattaaaaa actgaaagtg tacgctggtg cggagcatgc
    17701 ccatgctgcg caacaaccca aagttttgga actgaaataa taaggacacg acatgaacgg
    17761 taaatactac tacggcacag gccgccgcaa aagttcagtg gctcgtgtat tcctgactaa
    17821 aggtacgggt caaatcatcg taaacggccg tcccgttgac gaattcttcg cacgcgaaac
    17881 cagccgtatg gttgtccgcc aacctctggt tctgactgaa aacgccgaat ctctcgacat
    17941 caaagtaaat gttgtcggcg gcggcgaaac cggccagtcc ggcgcaatcc gccacggtat
    18001 tacccgtgcc ctgattgatt ttgacgctgc tttgaaacct gccttgtctc aagccggttt
    18061 cgttacccgc gatgcgcgcg aagtcgaacg taaaaaaccg ggtctgcgca aagcacgccg
    18121 tgcaaaacaa ttctccaaac gttgattttg gaaattcaaa aaaccctgct tatcgcaggg
    18181 ttttttattt gtaacaggcg gtttcccatt ggtaatctaa agattacgga ttgggcaaaa
    18241 atcaaaaaca gcaccagtat cgactacacg gacgaactga ccgtcaggga tggctgttac
    18301 tggagaggac atcgtatttc tactttgtaa aataactttt aatttattta gagttattga
    18361 aaaaatagaa cggctatttc aactagccta taaaaatggc aatctattag ggttagcata
    18421 ggtgtcaccg ccgccgttca agccgatcag taaaggaaca gtttcctagt ttttagctaa
    18481 cctgtattat ataatgggga gaatatctta aataaccaag catccttaat cataatttag
    18541 agaaattatt ttttcagagt ctaacccttt taatatgatt aacttattaa ctttatctat
    18601 ttcccagtct gatattatat cggtagatac tgaattctct cttattaaat ttttatctgt
    18661 aataattgca cccaactcat gaataataca aattttttga ttatccaata aataaatatc
    18721 aaaaactatt ccatctatat ttagtatgtt aacttttgaa ttttgtatag aaatacaaaa
    18781 aacgactgaa tcaaatccga taaaaatatt tttatcatca agcattaata cttttggggg
    18841 aatacttatg caagaaacag ctaatccaat acctatattg aattctttat aaaaagaaag
    18901 aataaaacat cgataaatct cttttcctcc ctcttgtaga ataatatatt tttgataggt
    18961 gtttattaaa tagtcttcta actctcttat ggagttataa ggatatttcg ttaatctcat
    19021 tattttgctc catgtctaaa acgtcctgtt gatccatata tttcacccgt tatgagtctg
    19081 ttcccatctc atatagtttc aagtttaaca accccttgag gctatgttaa aagagttttt
    19141 ctaataacta ttccgttttc ttaaatataa aaaaatagaa aaatagcccg ggtatagttt
    19201 gaacaaggga aatcagaccg gttccaactc acttaatccg ggcaagtccg cgaagccgcg
    19261 ttcacgtatg atttggcaaa agttgttcag ataactttta tctgtgtctt cagtgcggat
    19321 ggcggcatac agtttgcttt gcagtccgtc ggcagtaatt tggcggtgga cgacatagcc
    19381 tttttcaagg tagggcatga ctgtccaata gggaagggcg gcaatgccac gtctgctggc
    19441 aaccagttgg ataatggcga tggtcagctc gctgtgccgg cgcggcgggt tgatgttttt
    19501 cggaatcagg atttttttgg gtaaatccag catttcatcg ggaacgggat aggtaatcag
    19561 ggtttccccg ataaagtctt ccgccgtcca aacgtttttg gcggcaagcg gatggtccgg
    19621 ggcgcaaatg ccgaccattt cgtaggcaaa cagtgggtgg aagctgatgc tgctttgttt
    19681 ttcggcttcg gagacaatgg cgaggtcggc gcggtgttgc agcagcagcc cgacgggatc
    19741 cgcttggaat cctgatacga tatccagttc gacttggggc cacatcgggc ggaattcgcc
    19801 catggcgggc atcagccagt cgaaacaggt atggcattcg acggcaatcc gcagctctcc
    19861 cgcctcccct tccgtgattc gcgccaaatc tcgttctgca acagcaactt gaggtatcag
    19921 ttcgtgggcg aggcgcagca gcctttcgcc caccggggta aagcgcaggg gcgtggattt
    19981 gcgttcgaac agcggcgtgc cgtagtggtt ttcgagcata cggatctggt gggaaagggc
    20041 ggattgggta aggaaaaccc gtttggcggc aagggagacg ctgccggttt cttcaagtgc
    20101 cagcagggtt ttgaggtggc gcaattcgat aatggaatcc atggggcttc agacggcata
    20161 ttgaaccggc gcatattaaa ataattcata tgcgggtgca aatcggaaaa atgggcgttt
    20221 cgggcatttg cggttagaat gcccgcttgt ttggaaatga caggaaaaat atggttttgc
    20281 gcagagattt tttagcctgg tgcgacgaaa cgttgcagac ggcatcgttt aaagattacg
    20341 cccccaatgg tttgcaggtt gaagggaggg aatatatcgg gaaaatcgtt acgtcggtaa
    20401 cggcaagcag ggcagcgatt gattttgctg tggagcagaa ggcagatttg cttttggtac
    20461 atcacggtat gttctggaaa aacgagctgc cgaccgttac cggttggaaa aaagaacgga
    20521 ttgccgcact gttacggcac gacatcaata tggcaggcta ccatctgccc ctggatgtgc
    20581 atcccatact gggcaacaat gcccaactcg ccgacagatt gggttttgcg acagaaaaac
    20641 ggttcggcga acaaaacctg ctcaactcgg gcagcctgaa acaagccaag acactcggcg
    20701 cattggcggc gcatattgaa acagttttgc aacgtaagcc tgtcgccatc ggcaatcccg
    20761 aacgcgaaat ccgacgggtt gcatggtgta ccggcggtgc acagggcttt tttcagacgg
    20821 caatagatga aggcgtcgat ctgtatctga cgggtgaaat ttccgaagcc caataccacc
    20881 tcgccaatga aacgggtacg gctttcattt cggcagggca tcacgcgacg gaacgttacg
    20941 gcgtacgcgc gctggcagaa tcggcggcag aggttttcgg gttggaagtg tgccattttg
    21001 acgaaaacaa cccggcttga gtcttgagaa atatcataaa actttacctt attttaaata
    21061 atgctttgag tatcactgca aactgggtaa aattgcaatg tttaatggct cggcattccc
    21121 aaaatattag gacgactgaa taatggataa tcaagaaatc aacaacggcc gccgccgttt
    21181 cctgacactt gcgacctgcg gcgcgggcgg agtggcagca ttgggtgtgg caacgccgtt
    21241 tgtggccagt tttttccctt cggaaaaagc caaggccgcc ggtgctgccg tcgaggtgga
    21301 tgtcagtaaa atcgaagcgg gtcagctgct gaccgccgag tggcaaggca aaccgatttg
    21361 ggtgctcaac cgtacagatc agcagcttaa agacctgaaa ggcctgaacg gcgaacttac
    21421 cgatcccaat tccgatgcgg aacagcagcc ggagtatgct aaaaacgaga cccgttcgat
    21481 taagccgaac atccttgtcg ccatcggtat ctgcacccat ttgggctgct cgcccacctt
    21541 ccgtcccgac attgcccccg ccgatttggg cgcagactgg aagggcggct tcttctgccc
    21601 gtgccacggt tcgaaattcg acttggccgg ccgcgtatat aaaggtgttc ctgccccgac
    21661 caacctggtt gtcccgccat ataaatactt gagcgacaca actatcttgg tgggcgaaga
    21721 ctaagaataa ggaacgataa ttatggcaaa ccaaaccaat agcaaagcaa aagcattgtt
    21781 aggctggatg gacgctcgct tccccctgtc taaaatgtgg aatgagcatt tggcgcaata
    21841 ctatgcgccg aaaaacttta atttttggta ctatttcggc tctttagccc tgctcgtcct
    21901 cgtgattcaa atcgtcagcg gtattttcct gacgatgaac tacaaaccgg acggcaacct
    21961 gaatgcctac catctgcctg ctgcctttac cgcagtagag tacatcatgc gcgacgtgtc
    22021 cggcggttgg attatccgct acatgcactc caccggcgca tctttcttct tcattgttgt
    22081 ttatctgcac atgttccgtg gtttgattta cggttcatat aaaaaaccgc gcgaattggt
    22141 gtggattttc ggttctttga tcttcttggc attgatggcg gaagccttta tgggctacct
    22201 gctgccttgg ggtcagatgt ccttttgggg tgcgcaggtg attatcaacc tgttctccgc
    22261 cattcccgtc atcggtcctg atttgtccac ttggattcgc ggcgacttca acgtttccga
    22321 cgttaccctg aaccgcttct tcgccctgca cgttatcgcc gtaccgctgg tattgctcgg
    22381 tttagtcgtg gcacacatca tcgcactgca cgaagtgggc tctaacaacc ccgacggtgt
    22441 ggaaatcaag aaaaacaaag atgaaaacgg cattccgcgc gacggcatcc cgttccaccc
    22501 ttactacact gttaaagaca tcctgggcgt cgttgtattc ctgattgtct tctgtgccgt
    22561 attgttcttt gcccctgaag gcggcggcta cttcctcgaa gcgccgaact tcgatgcggc
    22621 aaatgcgctg aaaacacccc cgcacattgc gccggtatgg tatttcacac cgttctacgc
    22681 cattttgcgt gcgattcctt ccttcttggg aacgcaggtt tggggtgtaa tcggtatggg
    22741 cgcggcggtc gtgttgattg ccctgctgcc ttggctggac aggggcgagg tgaaatccgt
    22801 ccgttaccgc ggtccgattt tcaaaaccgc attggtcttg ttcatcattt cctttatcgg
    22861 tttgggtatt ttgggcgcga tggtggcaac cgatacgcgt actttggtcg cgcgcatcct
    22921 gtccttcgtc tatttcgcat tcttcctggg tatgccgttc tataccaagc tggataaaaa
    22981 caaaccggtt cccgaacgcg taaccatgag caccgccaaa caaaaaatca tgttctttgt
    23041 ttacgtcggc attactgccg tgggtgctta cctgtttgca accaacatct gatgagggca
    23101 gtcgaaatga aacaagctat gaagaattgg tttgctgcct tgctgtcggc agtacccatg
    23161 agtgcggcat ttgcttcggg cggtcatgca cactatgaga aagtcgatat tgatttgcgc
    23221 gaccaagtaa gcttgcagcg cggcgcacaa atctttacca actactgttt gtcctgccac
    23281 tcggcaagcg gtatgcgttt caaccgtttg aaagacatcg gtctgactga tgaagaaatc
    23341 aagaaaaacc tgatgtttac caccgataat gtcggcgatg tcatgcattc ggcgatgaac
    23401 ccgaaagatg cggcaaaatg gtttggtgct gctccgcccg atttgacatt gattgcgcgt
    23461 tccaaaggtg cagactacct ttacgcctat atgcgcggct tctataaaga tccgacccgt
    23521 ccgagcggct ggaacaatac cgtattcgat aaagtcggta tgccccaccc gttgtgggaa
    23581 cagcaaggtg ttcaggcagt cgagttggat gccaaaggtc agccggttat ggtaaaagac
    23641 gaacacggcg agatgaagcc taagctgtat tgggaatcta ccggcctgca cagccgccgc
    23701 ctgccgaacg gcaaagtcat ccaaaaagag tacgatactt atgtacgcga tttggtcaat
    23761 taccttgtgt acatgggtga acctgcacaa ctgcaacgca aacgtatagg ctatgtcgtg
    23821 atgattttct tgttggcggt tatgctgcct ttggcatact tcctgaaaaa agaatattgg
    23881 aaagacgtac actaagcgtt tggaacaaaa gggcaaatcc tttagggttt gccctttttg
    23941 catgcttctg ccctttggga aaatcagcac ccggatgccg tctgaacata gctgcaacat
    24001 ttcagacggc atcattctaa aaatgtcaga catcgggaac ttaatcaggg tttgtggcag
    24061 attgatattg aattaggaaa aggtctgaaa ccgcaagcag catggtaagg cgatgtcgaa
    24121 caggtttaaa gccaatccgc tatatttttt ggagctgcac cgtgcaaaca ggtagaatgg
    24181 cggcggtttt gtccggtgtg aaaatgtttg aatattagga tgattgatgg agctgatgac
    24241 tgttttgctg cctttggcgg cgttggtgtc gggcgtgttg tttacatggt tgctgatgaa
    24301 gggcaggttt cagggcgagt ttgccggttt gaacgcgcaa ttggcggaaa aggcggcaag
    24361 atgtgatttt attgaacagg cacacgcaga aattgcatca gaattggccg ctttggatgg
    24421 gaaatacgag catttgcagg acgaaaatta tgctttgggc aaccgttttt ccgcagccga
    24481 aaagcagatt gcccatttgc aggaaaaaga ggcggaatcg gttcggttga agcagtcgta
    24541 tatcgatttg caggaaaagg cgcagggttt ggcggttgaa aacgaacgtt tggcaacgca
    24601 gctcgggcag gaacggaagg cgtttgccga gcaatatgcg ctggaacgcc aaatccgcca
    24661 aagagtcgaa accgatttgg aagaaagccg ccaaactgtc cgcgacgtgc aaaacgacct
    24721 ttccgatgtg ggcaaccgtt ttgccgccgc cgaaaaacag attgcctatt tgcaggaaaa
    24781 agaggcggaa gcggagcggt tgaggcagtc gcataccgag ttgcaggaaa aggcgcaggg
    24841 tttggcggtt gaaaacgaac gtttggcaac gcaaatcgaa caggaacgcc ttgcttctga
    24901 agaaaagctg tccttgctgg gcgaggcgcg caaaagcttg agcgatcagt ttcaaaatct
    24961 tgccaacacg attttggaag aaaaaagccg ccgttttacc gagcagaacc gcgagcagct
    25021 ccatcaggtt ttgaacccgt tgaacgaacg catccacggt ttcggcgagt tggtcaggca
    25081 aacttatgat aaagaatcgc gcgagcggct gactttggaa aacgaattga aacggcttca
    25141 agggttgaat gcgcagctgc acagcgaggc aaaggccctg accaacgcgc tgaccggcac
    25201 gcagaacaag gttcagggca attggggcga gatgattctg gaaacggttt tggaaaattc
    25261 cggccttcag aaagggcggg aatatgtggt tcaggcggca tccgtccgaa aagaggaaga
    25321 cggcggcacg cgccgcctcc agcccgacgt tttggtcaac ctgcccgaca acaagcagat
    25381 tgtcattgat tccaaggttt cgctgacggc ttatgtgcgc tacacgcagg cggcggatgc
    25441 ggatgaggcg gcacgcgaac tggcggcgta tatcgccagc atacgcgcgc atatgaaagg
    25501 cttgtcgctg aaggattaca ccgatttgga aggtgtgaac acattggatt tcgtctttat
    25561 gtttatccct gtcgaaccgg cctacctgct ggcgttgcag aatgacgcgg gcttgttcca
    25621 agagtgtttc gacaaacgga ttatgctggt cggccccagt acgctgctgg cgactttgcg
    25681 gacggtggcg aatatttggc gcaacgagca gcaaaaccaa aacgcgctgg cgattgcgga
    25741 cgaaggcggc aagctgtacg acaagtttgt cggcttcgta cagacgctcg aaagcgtcgg
    25801 caaaggcatc gatcaggcgc aaaacagctt tcagacggca ttcaagcaac ttgccgaggg
    25861 acgcgggaat ctggtcggac gcgccgagaa actgcgtctg ttgggcgtga aggcaggcaa
    25921 acagcttcaa cgggatttgg tcgagcgtgc caacgaacag acggcatttg ctttgggcaa
    25981 accttcggaa caggaagcgg ctgacgaagc cgaataggaa aggcggatgc gtttcagacg
    26041 gcatcagtgc agatgatgcc gtctgaagac tttggaaagt cggttagaaa aacggattgt
    26101 gccgtttttc gtgtccgatg gaagtcatac gcccgtgtcc ggcgacaact tgcacggttt
    26161 cggggaaggc gaataatttg ttgcggatat tattgattaa gtcggcgtgg ttgccgcgcg
    26221 gaaaatcggt cctgcctatg gtttcgtaaa acagcacgtc gcccgcaatc agcagctccg
    26281 cttcggcaca atagaaaacc acgtgtccgg gcgtgtgccc cggaatatgc agcacttgga
    26341 aggcatagcg tccgaccgtg agcgtttcgc cttcttcgag ccaacggttc ggcgcaaagg
    26401 cgggcgagac ggggaatccg tattgcgcgg tggtttgcgg cagcgattgg agcaggaatt
    26461 catcttcgcg gtgcgggccg aggacgggga ctttatgcgt ttccagcatc tcgaccacgc
    26521 cgcccgcgtg atcgagatga ccgtgcgtca gccagattgc cgtgagcgtg agtttgcggt
    26581 ttgccaacgc ttgcagcagg aacggcacgt cgccgccgac atcggtcagg acggcttcgc
    26641 cgctttcgtc gtcccaaatc agggtgcagt tttggcggaa gggggtaacg gggagaattt
    26701 cgtaacgtag ggtcatcggc gtgttcctaa aacggttttt cagacggcat cgggtttgcc
    26761 gtttgtttta tggcggtttg ccgcctgttt tgatattggg ggaatcaggt gattaagaag
    26821 ataatcggcg gcatcatacc gatttttacg gcggttttca tccctgcatc ggcaggcgcg
    26881 gcggatttga tgctggcgca ggaatacaaa gggcaggaca ttgccggctg ggcgatgagc
    26941 gagaagctcg acggcgtgcg cgcgtattgg gacggaaagc acctgataag ccgtcagggt
    27001 tatgcgttta cgccccccaa aggctttacc gcgcagtttc cgccttatcc tttggacggc
    27061 gaattgtata gcggacgtgg tcagttcgag cagatttccg ctaccgtgcg ttctgtttct
    27121 tcagactggc gcggcatccg cctgcacgtt ttcgatgtac ccaaggcgca gggcaacctc
    27181 taccaacgtt tggcagtcgc aacgcagtgg ctgaaaacgc atccgaacgc gccgattacc
    27241 atcatcccgc aaatcaaagt gcgcgaccgg cggcacgcga tggacttttt aaaacaaatc
    27301 gaagcgcagg gcggcgaagg cgtgatgctg cgccagcccg aatcccgtta cagcggcggc
    27361 aggagcagcc aattattgaa gctgaaaagc caatacgacg acgaatgcac ggtaacgcgg
    27421 cactatgagg gcaaagggcg aaacgccgga cggctgggcg cggtcggctg caaaaaccga
    27481 cacggcgaat tccgcatcgg cagcggtttc aaggacaaag accgcgacaa cccgcccaaa
    27541 atcggcacgc tgattaccta ccgttaccgt ggctttacgc ggaaaggcac gccgaaattt
    27601 gccaccttcg tgcgcgtgcg taccgaccgt tgagcaggac ggctcggacg gcagccgata
    27661 ctttggttta taattttcct tttaccgacc gattccgaca tatgaccgat ttagaaacca
    27721 aacgccttga aacacaggcg atgcttgaaa acgccgaact tttgttcgac caagggcaat
    27781 gccgcgccgc actgcaaaaa gtggcgtacg agattacgcg cgatttgggg gataaatatc
    27841 cgttgctcct gcccgtgatg ggcggggcgg tggtgtttac ggggcagttg ctgccgctgt
    27901 tgcgctttcc cttagacttt gattatgttc acgtttcccg ttacggcgac aagctggagg
    27961 gcggcgcgtt caactggaag cgtatgcctg atgcggaaca aatccggggc aggcacgtgg
    28021 tcgtgttgga cgatattttg gacgaagggc atacgatgtc cgccattcaa gccaaacttt
    28081 tggaaatggg tgcggcaagc tgccgtgcgg cggtgttcgc caataaatta atcgacaaag
    28141 aaaaaccggt caaagccgat tatgtcggac tggatgtgcc gaaccgttat gttttcggtt
    28201 acggcatgga tgcggcgggc tgctggcgta atttgggcga gatttacgca ttgggcggaa
    28261 aataagggat gcgatgccgt ctgaaggcct ttcagacggc atcgcgccca tacgccggca
    28321 ggattaatga ggaacaggac gcatcaatat gatagggctt ttaatcatca cacacgaaac
    28381 cataggcgaa gcctaccgca agctggcgca tcattttttt ccgggcgggc tgcctgaaaa
    28441 cgtccgcata ctcggcgtgc agccgacgga aggccaagac gacattatca acaacgccat
    28501 ttccgcgctg caagagtttc ctgaaaacca cggtgtattg attatgaccg acatcttcgg
    28561 cgcgaccccc tgcaatgccg cccgccggct cgtgcgcgaa aacaaatcgg cgattttgac
    28621 cgggctgaac gcgccgatga tgattaaggc cgtccaatat tcgccggcgg cggaagactt
    28681 tgccgccttt accgaatgcg tcagggaggc ggcggtaaaa ggcattttcg ccatcacgtc
    28741 cgcgcccgaa gatttggtgt gccggcgcag cggcgatgcc gtctgaagaa gcggcagggc
    28801 aggaaaacat tttaaacggc gtctgctgcc gatatatagc ggattaacaa aaatcaggac
    28861 aaggcggcgg gccgcagaca gtacggatag tacggaaccg gttcgcccgg tgcttcagca
    28921 ccttggagaa tcgttccctt tgagccaagg cggggcaacg ccgtactggt ttttgttaat
    28981 ccactataca taacacggga accgaaatgc tcaaacaatc catcgaaatc atcaacaaac
    29041 tcggactcca cgcccgcgcg tccagcaagt tcacccaaac cgcgtcccaa ttcaaaagcg
    29101 aagtctgggt tacgaaaaac ggcagccgcg tcaacggcaa aagcattatg gggctgatga
    29161 tgctcgcagc cgccaagggt acggtcatcg aactggaaac cgacggcttg gacgaagccg
    29221 cagccatgaa ggcattgacc gacttaatca acgactactt cggcgagggc gaataatgag
    29281 tatcgtgctg cacggcgtgg cggcgggcaa aggcattgcc gtcggttgcg cccacctgat
    29341 tgcgcgcggt acggaggaag tgccgcagta tgatgttgct caggcggaca ccgatgccga
    29401 agccgaacgt ttcgatgccg ccgtcaaagc cacgcgcaaa gagttggaac agctccgcag
    29461 cgcgattccc gaaaacgccc cgaccgagtt gggcgcgttc atctcgctgc acctgatgct
    29521 cttgaccgat gttaccttgt cgcgcgaacc cgtcgatatt ttaagggaac aaaaaatcaa
    29581 cgccgagtgg gcattgaagc agcagagcga caaactcgcc gcccaattcg acaatatgga
    29641 cgatgcctat ttgcgcgaac gcaagcagga tatgctgcaa gtcgtccgcc gcatccacaa
    29701 caacctgatc gggcagggca acgagttgga ggttgccgac aacctgtttg acgaaaccgt
    29761 tctgattgca aacgaccttt cacccgccga cacggttttg tttaaagagc agcgcattgc
    29821 cgccttcgtt accgatgccg gcggccccac cgggcatacg gcgattttgg gcaggagctt
    29881 ggacatcccg tccgtcgtcg ggctgcacaa cgcgcgcaaa ctgattaccg aaggcgaaac
    29941 ggtcatcgtg gacggcatca acggcgtgtt gattatttcg ccggatgagt cggtgttgaa
    30001 cgaataccgc cgccgcgccc gcgaataccg cagccacaaa cgcgatttga acaagctcaa
    30061 aaaaaccgcc gccgccaccg ccgacggggt ctgcatcgag cttgtgggca atatagaatc
    30121 cgccgaagac gtgaaaccgc tgcacaacct cggcgcagac ggcatcgggc tgttccgcag
    30181 cgagtttctt tacctgaacc gcgatacgat gccgtctgaa gacgagcagt acgaagtgta
    30241 cagcgcgatt gtcaaaaaaa tgaagggcaa aagcgtaacg atacggacag tcgatttggg
    30301 tgtggacaaa aatccgcgct ggttcgggaa aaacagcacg cccaacggca gcctcaaccc
    30361 cgcgctgggc atgaccggca tccgcctgtg ccttgccgaa ccggtcatgt tccgcacgca
    30421 gatgcgcgcc atcctccgtg cggcggtaca cggtcccgtc cgcatgatgt ggccgatgat
    30481 tacctccgta tccgaagtgc gccagtgcct catccacctc gacaccgcgc aacgccagct
    30541 tgccgaacgc ggcgatgcct tcggcgaagt cggcatcggc tgcatgattg aaattccgtc
    30601 tgccgcgctg accgtcggca gtattttgaa actggtcgat ttcatctccg tcggtaccaa
    30661 cgatttgatc caatatattc tgtccgtcga tcgcggcgac gacagcgtca gccatctcta
    30721 ccaacccgga catccttccg tgctgaaaat gctgcaacac gtcatccgta ccgccaaccg
    30781 catggacaaa gacgtgtccg tatgcggcga gatggcgggc gataccgcgt ttacccgcgt
    30841 tttattgggt atggggctgc gccgtttttc catgaatccg aacaacatcc tgcccgtcaa
    30901 aaacatcatt ctgcacagca atgccggaca gctcgaaggc gatattgcga aagtcatccg
    30961 ctgcgaagac gaagagaagt cggaaaagct gatcaaacag atcaacagcg tgtctgtcgc
    31021 ggaagaagcc gaagtcaagg gacggaaata aatattgttg acaatgcgag cgggttgcag
    31081 tcaaataacg cccttaaaaa atacagctgc tttggtcaag aaatgccgtc tgaaaaattt
    31141 tcagacggca ttttgtttgg caggcatttc tgccgcatca tcggaaacgg acgatacgcc
    31201 cttcatcgac gcggatgcgg accgtaccgt ttccggaaat accgtccgta tgttgtccga
    31261 cagtcaggtt taaggtcagc tcgccgtgtt cgggatggac ggcggaaagc cggagcgagt
    31321 cgggcaggcg gacgagggac agcaggcggc attccgttcc atgattgtcc aagcacacgg
    31381 cattttgcgg aatatggcgg tcatcgtcgg tattgggcag ccccatcaga cgggcgacct
    31441 gcacgccggc aggcgtttga atcaaggttt cgggcgtacc gcattgaagg attttcccct
    31501 cgtgcatgac ggcgatttcg tccgccgccg tgcaggcctc ttcgggcgaa tgcgttacca
    31561 aaacggcagg gatgccgccc ttgcggatgc gttcggcggt catacggcgc agccggtcgc
    31621 gcaaatgcgt gtccaaactg gaaaacgatt catccagcaa cagcagggaa gggcggacaa
    31681 ccaaagcgcg cgccagtgcc aaccgttgct tctcgcctcc ggaaagtttt tcaggcttgc
    31741 ggtgcgcctc gttttccagc ccgacttcgg caagtgccga caaggcgagg cgttcggctt
    31801 cggctttcgg cattttttgc attttcaaac cgaatgccgt attttccagc gcactcatat
    31861 ggggaaacag cgcgtaatct tgaaacatca gcgagatacg gcgtttttcg ggcggcatac
    31921 aggtaatgtt ttccccgttc agccgaattt cgccgccgtc cggccggacg atgcccgcaa
    31981 tcatattcag cagggtggat ttgccgcagc ccgaccgccc cagtacggcg agtattttgc
    32041 cgcgcccgac agtcaggcag atgttgtcgg cgaccgtttt gccgccgaag catttgcaga
    32101 gtccgttcag ttcaagcatg gcgcatccta taaacgtatg ccgtccagcc actcggacag
    32161 cggatgggcg gcatcaatca gaccgtaacg gtaaaacgcg tccagcgtaa ccagttgcgc
    32221 gtcgtgcatc atgtttttcg acaacatggc atccaatagg ccgccaatgt ccatcttttc
    32281 aaaacccgct acctcgccat cctgattttc aggcaggaag gtttcgggca ggacggcatc
    32341 gaacacatac aggatttcat tgtgcacacc tcggctgacg gggcgaaggc tgtgcagccg
    32401 cgatactggg cggatgagcg gaaacagcgt tttatccaaa ccggcttctt cgctgctttc
    32461 gcggcacacg gcttcagacg gcatttcgcc gccggaaaca ccgccgccgg caatattgtc
    32521 gagcttgccg ggatcgactg ctttgtgcgg actgcgcctg cctatccaaa aatgccatct
    32581 gccgttcgat tcgaccaaac cgttgagatg gacggcgcgg ctgagtagtc cgaacggacg
    32641 gaaagcggcg cgttcgagcg tgaacaaggg gttgccgccg ccgtcggtca ggtcgaaaca
    32701 ctcgttgcgc catccgtgaa gcagccccgc cttgttccat gtgcgggcga ggtgctgcaa
    32761 gcgtccgccc atatccggcc agccgtccgc attcagaaaa atgccgtctg aagactcgga
    32821 gcagcctgcc tcccagtctt ttttgatgcg ctcagcccat tccggcgaca gattgcccaa
    32881 aggaagacgg ttcagataca gcgttttcca gcaactttcg gcaccgtaac ttgcttttgc
    32941 ccgctcgaac agggcatcaa ggtcttgttt gctgacggat tcggtaaaac ggacggtcgg
    33001 catagccggc tttcttgaac acaatgcgga agattgtagc caaaccgcct gaatttgcaa
    33061 ccgcaaacag acggcggcct gccgttccga tattgcggca ggaaggaaaa tccggcaaaa
    33121 aacggaggcg gcaggaaaca ggaaaaacaa aaccccctcg gcattgaacc gaggggtttg
    33181 atatttggtt gcgggagcag gattcgaacc tacgaccttc gggttatgag cccgacgagc
    33241 taccatgctg ctccatcccg cgtcagaaga tgaaactata cggcagattt tttatgttgt
    33301 caaacattat ttccgtgaaa aatcataaag ttttccgtcc ggcggcttat gcctgatttg
    33361 aaatatcatt ttctttacaa aagttcatgt tcgtgattta attttggtta acatcgaaac
    33421 aggggtgctg cctgatgttc aggcggctga gaaataccct ttacacccga tcgggataat
    33481 acctgcgtgg ggagttttca cggattctgt ctttcagacg gcattggttt tcaaatgccg
    33541 tctgaaaacg caaaacgctc ctgtttcttt attctaaacg agaaaacagg agcatttttt
    33601 atgactacgc caaaaaaaac cgccaaaatt tccggcaacg aagcgcgcga actttccgac
    33661 ttgagcgaag acatcggcat ccgcttcaaa tatcagaact ccgaacgcgt gtatctgcaa
    33721 ggcagccgcg acgacatccg cgtacctttg cgcgaaatcc gtcaggacga cacctacacg
    33781 gcgcaaggta cggaagccaa cccgcctatt ccggtgtacg acaccagcgg cgcatacggc
    33841 gatccggcgg cgcacatcga cctgaaacaa ggcctgccgc acatccgcac ggcgtggctg
    33901 gacgaacgcg gcgataccga aatcctgccc aaactctcca gcgaatacgg catcgaatgc
    33961 gcacacgatc cgaaaaccgc ccatctgcgc ttcaaccaaa tcacccgccc gcgccgtgcc
    34021 aaagccggcc gcaatgtaac ccagcttcac tacgcgcgcc aaggcattat cacgcccgaa
    34081 atggagttcg ccgccatacg cgaacgcatg aagctggacg agcttttcag acggcctgaa
    34141 tacgccaagc tcttgaaaca gcacacaggg caaagtttcg gtgcgaacat cccgacccgt
    34201 cccgaccaaa tcacccccga attcgtgcgc caagaaatcg ccgccggacg cgcgattatc
    34261 cccgccaaca tcaaccaccc cgaactcgaa ccgatgatta tcggccgcaa cttccgcgtc
    34321 aaaatcaacg gcaacttggg caattccgcc gtaacctcca gcctgaccga agaagtcgaa
    34381 aaaatggtgt ggtcgctgcg ttggggcgcg gacacgatta tggacttatc cacaggcgcg
    34441 cacatccacg aaacgcgcga atggattatc cgcaacgcgc ccgtccccat cggcacagtg
    34501 ccgatttacc aaactttgga aaaaaccggc ggcatcgccg aagatttgac ttgggatttg
    34561 ttccgcgaca ccttaatcga gcaggcggaa caaggcgtgg actatttcac catacacgcg
    34621 ggcgtgttgc tgcattatgt gccgatgacc gccaaccgcc tcaccggcat cgtatcgcgc
    34681 ggcggttcga ttatggcgaa atggtgtttg gcacatcatc gggaaaactt cctctacacg
    34741 catttcgacg aaatctgcga aatcatgaag gcgtatgacg tatcgttcag cttgggcgac
    34801 ggcctgcgcc ccggctgcat tgccgatgcc aacgacgaat cccaattcgc cgaattgcac
    34861 accttgggcg aattgaccga taaagcgtgg aaacacgacg tacaagtcat gatcgaaggc
    34921 cccggccatg tgccgctgca acgcgtcaaa gaaaatatga ccgaagagtt gcaacactgc
    34981 tttgaagcac cgttttacac actcggcccg ctcgttaccg acatcgcccc cggctacgac
    35041 cacatcactt cgggcatagg cgcggccaat atcggctggt acggcacggc gatgctttgt
    35101 tacgttaccc ccaaagaaca tttggggctg cccgacaaag aagacgtgcg caccggcatc
    35161 atcacctaca aactcgccgc ccacgccgcc gacctcgcca aaggctggcc gggcgcgcaa
    35221 ttacgcgaca acgccctgag caaggcgcgt ttcgaattcc gctggcgcga ccaattccgc
    35281 ttaagcctcg accccgaacg cgccgagagc ttccacgacg aaaccctgcc cgccgaaggc
    35341 gcgaaaatcg cccatttttg ctcaatgtgc ggccccaaat tttgctcgat gaaaatcacg
    35401 caggaagtgc gcgactacgc cgacaagcaa aaagcccaac ggcagggtat ggaggaaaaa
    35461 gcggtcgagt tcgtcaaaaa aggcgcgaag atttacagtt gaaacgtcaa gcaaaaaatg
    35521 ccgtctgaag cccggaaaaa aggcttcgga cggcattctt tcgcttgtga aaatgattaa
    35581 acaaccgatc attaaagtaa tttttaaaat ttttatattc acatagatgg gaatgatgga
    35641 tttagggttc tgattttgtt ttgagagaat gaaggaattt gagattgtgg gcgattatcg
//
LOCUS       DAXDSD010000007        35191 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXDSD010000007 DAXDSD010000000
VERSION     DAXDSD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35191
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.007"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            112..2139
                     /locus_tag="ACFB2Q_000245"
     CDS             112..2139
                     /locus_tag="ACFB2Q_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC0917365.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSGLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLLKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(2228..3781)
                     /locus_tag="ACFB2Q_000246"
     CDS             complement(2228..3781)
                     /locus_tag="ACFB2Q_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC0917366.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     DRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            3832..3960
                     /locus_tag="ACFB2Q_000247"
     CDS             3832..3960
                     /locus_tag="ACFB2Q_000247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917367.1"
                     /translation="MPSENGRRQFIIFAFRRHIKRRLCAACRRFAPVLPMSDLNAV"
     gene            complement(4032..5225)
                     /locus_tag="ACFB2Q_000248"
     CDS             complement(4032..5225)
                     /locus_tag="ACFB2Q_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC0917368.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHSGNWQSFTSRNNTGDFPLGGILAQTGSGGGMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(5222..6205)
                     /gene="patB"
                     /locus_tag="ACFB2Q_000249"
     CDS             complement(5222..6205)
                     /gene="patB"
                     /locus_tag="ACFB2Q_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC0917369.1"
                     /translation="MKNFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQDTEAAAIRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(6216..7652)
                     /locus_tag="ACFB2Q_000250"
     CDS             complement(6216..7652)
                     /locus_tag="ACFB2Q_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC0917370.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGILAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWLITFHFVCLTFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            complement(7782..8947)
                     /locus_tag="ACFB2Q_000251"
                     /pseudo
     CDS             complement(7782..8947)
                     /locus_tag="ACFB2Q_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            9388..9600
                     /locus_tag="ACFB2Q_000252"
     CDS             9388..9600
                     /locus_tag="ACFB2Q_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC0917371.1"
                     /translation="METLLLDIGGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            complement(9579..9740)
                     /locus_tag="ACFB2Q_000253"
     CDS             complement(9579..9740)
                     /locus_tag="ACFB2Q_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917372.1"
                     /translation="MSEGTIARKFNQFILPVQLNQMPPEGAERASDGIGETQRLQTAF
                     LPLYFNAAS"
     gene            9748..11327
                     /locus_tag="ACFB2Q_000254"
                     /pseudo
     CDS             9748..11327
                     /locus_tag="ACFB2Q_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
     gene            11409..12506
                     /locus_tag="ACFB2Q_000255"
     CDS             11409..12506
                     /locus_tag="ACFB2Q_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC0917373.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQGPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            12620..13734
                     /locus_tag="ACFB2Q_000256"
                     /pseudo
     CDS             12620..13734
                     /locus_tag="ACFB2Q_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            13802..14314
                     /locus_tag="ACFB2Q_000257"
     CDS             13802..14314
                     /locus_tag="ACFB2Q_000257"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC0917374.1"
                     /translation="MPSERVSASDGIALEQGWDMKKPKILFVCLGNICRSPMAEYILR
                     RRAAEAGIPLETDSAGTSGWHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFD
                     YIIAMDGKNLSELEKTFGRRPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAG
                     CRALLEKISK"
     gene            14391..14867
                     /locus_tag="ACFB2Q_000258"
     CDS             14391..14867
                     /locus_tag="ACFB2Q_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC0917375.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            14855..15781
                     /locus_tag="ACFB2Q_000259"
     CDS             14855..15781
                     /locus_tag="ACFB2Q_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC0917376.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAAGKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            15846..16403
                     /locus_tag="ACFB2Q_000260"
     CDS             15846..16403
                     /locus_tag="ACFB2Q_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC0917377.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGLYQNVPVQPVKIRRVVVGQ"
     gene            16511..18562
                     /locus_tag="ACFB2Q_000261"
     CDS             16511..18562
                     /locus_tag="ACFB2Q_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC0917378.1"
                     /translation="MLKCCQPNPTQPNPTQPNPSPDLSDTAGANTEAIYTSDGITANS
                     TQLEQLKKLFPACFDADGNFLIDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNLPP
                     ETLISEDKTHNAKPENAGSQNLLIRGDNLEVLKHLKNAYANSVKMIYIDPPYNTGSDG
                     FVYQDDRKFTPAELALLANIDEDEAARILDFTDKGSNSHSAWLTFMYPRLYIARELLK
                     DDGVIFISIDDNEAAQLKLLCDEVFGEGNFVAQLPWRKRTAKSDVPFGISQDYEWIFV
                     FAKSCQFIAATKGKERRYYETDDFPDRPWRTHDLTKQTTAAERPNSFFTMVDPKTGKK
                     YPANPNATWRVTKDTFQDYYNKGKIVFPDDYDFLNISNPVMRYFKDDDMKKAGEDFGK
                     VAVSSRLPENVGTLADAVAEYLAIFSRTLPENIGMTKEGTKEITDLFGSKIFTFPKPS
                     QLIKFLVSISSKSNDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKS
                     EARKAGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPE
                     QPDLPLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGSYTAYLCEKRLYLLNKGF
                     TSADLLAFIKKLDDDADFNPNRVIVFGSNMASAMQHELDQAVRGYTNKKEIELNVIIR
                     V"
     gene            18652..21591
                     /locus_tag="ACFB2Q_000262"
     CDS             18652..21591
                     /locus_tag="ACFB2Q_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFC0917379.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIQMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENEH"
     gene            complement(21685..22644)
                     /gene="ttcA"
                     /locus_tag="ACFB2Q_000263"
     CDS             complement(21685..22644)
                     /gene="ttcA"
                     /locus_tag="ACFB2Q_000263"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC0917380.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(22736..23599)
                     /locus_tag="ACFB2Q_000264"
     CDS             complement(22736..23599)
                     /locus_tag="ACFB2Q_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC0917381.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAK"
     gene            complement(23634..23996)
                     /locus_tag="ACFB2Q_000265"
     CDS             complement(23634..23996)
                     /locus_tag="ACFB2Q_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC0917382.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            24071..24550
                     /gene="ybaK"
                     /locus_tag="ACFB2Q_000266"
     CDS             24071..24550
                     /gene="ybaK"
                     /locus_tag="ACFB2Q_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC0917383.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            24572..25264
                     /locus_tag="ACFB2Q_000267"
     CDS             24572..25264
                     /locus_tag="ACFB2Q_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC0917384.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNLQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIMQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLVIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            25268..26143
                     /locus_tag="ACFB2Q_000268"
     CDS             25268..26143
                     /locus_tag="ACFB2Q_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC0917385.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
     gene            26351..29779
                     /locus_tag="ACFB2Q_000269"
     CDS             26351..29779
                     /locus_tag="ACFB2Q_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFC0917386.1"
                     /translation="MMSGFSPKPKTIILSLAGAFGALAFADTPNNTEQQKELNTIVVH
                     GKRSADQKGADDVYYKNVSNAYVGKEYLERYRVQSAGDVLKGLNGVYNMNTRTAGGAI
                     TPNIRGITGKGRIPVTIDGTEQTIDVWMNNYGVGDRNYLDPALFRSIAVEKSPALTRG
                     VKSGVGGAMSIRTIEAGDIVPEGQKFGFQLKTEFANNRTRPANNLNQWLGWEDYRTLP
                     LGATADGAGGGSDPITGQQSPQALVVDNFTPPQHKSGRDNWRFGGDRSYMAAAAFKTE
                     LSDGLAAYSYRNKGNYFAGRKGAEGYLNNPVYDLQKCYDEGGSDFNCKNSATFIPNMA
                     RIYHPGVEVLNSNTETKTLLLKNNWHLPGSHRLGWQYMRTDVRFGEINPFHTTYVMNM
                     EEHNETNRPKELSPQAQSIDSTIRTDTYKLGWAWKPENSRWVDLQANLWRIKTNSTRH
                     QSGGMDLSSARPDPFYDVWYWCTQRGRIPPEHVDNYSSCNDLMNDFGVNGLTKEQVLA
                     MTPNDNGQFRVISGAEQKTRVSRTGFDISNRFRLRDRLSMTLAADYQKEKLAEEVEIV
                     NSQDLFNLAGMVTGLTKLAGPRGGERREWGTNLVFDWQAADRLKISAGIRYHNFRGFD
                     TALAEGRARRDPRYQAGDGTQHYADGAYLPYFELAGDQERRDWNVVAEQLRQAYQSGD
                     AAAIAAAVQAEQAHGARYHLLKYYDGSYRTDGYVYIGNDGRAYFDGRFGGVNEAQNQP
                     LYRVRPVYVPFVNGKLDSGALPQHFHDFVSNYQEKVTNPQGLHGTHYRYWAGVGNPID
                     CIRSDAASCLAHNLSGKVTNFQVGYPEYGDDTGLGLKIIGRHYTEEQYWAQPKPIRAH
                     AWAPTIAVSYDLTDNSRLFVRYAQMSRFPSVYEVGSFYNDVAYAGMPKAPNFRFKPER
                     SRSWEIGYSFNFAPYWSRLRAGDMRLTYYRNRIQNVIETTDYFRTVQYDRKDTAGLEW
                     QSRIDTGRFFAALGATYRLKQQMCDRDTAFDYDPYGAKGVPICIEGGYGSTRGYQALQ
                     PKYSINLDAGVRLLGEKLELGLRGIYHSRVNTKQYDQLLQKDLGIIFDTTGKPHHWRS
                     SLTWDVYGRYRLHKNLNVNLGITNLTNRYYLDPMSNVAAPGPGRTVTFGLTAKF"
     gene            29994..30977
                     /locus_tag="ACFB2Q_000270"
     CDS             29994..30977
                     /locus_tag="ACFB2Q_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
                     /protein_id="HFC0917387.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            31248..32738
                     /locus_tag="ACFB2Q_000271"
     CDS             31248..32738
                     /locus_tag="ACFB2Q_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC0917388.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRRLLDEGSRQTQQYRES
                     GWLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARY
                     AKLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQ
                     NKESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGIN
                     QCVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGHHGVQVRGVLYGNRYTEKD
                     KDSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRT
                     LSPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITPKSGLLVNFDAARKA
                     YPEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRSDKQYIMM
                     AAAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(32806..34203)
                     /gene="aspA"
                     /locus_tag="ACFB2Q_000272"
     CDS             complement(32806..34203)
                     /gene="aspA"
                     /locus_tag="ACFB2Q_000272"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC0917389.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(34279..34416)
                     /locus_tag="ACFB2Q_000273"
     CDS             complement(34279..34416)
                     /locus_tag="ACFB2Q_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917390.1"
                     /translation="MAEHNPPKDILAAGGACRKVRKVTTENNVSRFQTAYDMDNFIRP
                     V"
     gene            complement(34480..35040)
                     /locus_tag="ACFB2Q_000274"
     CDS             complement(34480..35040)
                     /locus_tag="ACFB2Q_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC0917391.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQPFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
BASE COUNT         8523 a         9032 c         9677 g         7959 t
ORIGIN      
        1 ttcggccggt ttgtgcattt cgtcatccgg aagccggtat tttgccggca ggttttgact
       61 actttgcccg atgaccgggt ttgtctgttt ttaacaaagg agtgagaagg tttgtccctg
      121 tctgaattta tagaacgccg aacgtcattt aatccgatgg ttattttgac gactttgttt
      181 tttgtgtgtg ttttggtggt attggtttta accgtgccgg atcaggtgca gatgtggctc
      241 gaccgggcaa aagaagtcat ttttaccgag ttcagctggt tttatgtttt aacgttttcc
      301 atttttctgg gttttctgct gatactctcg gtcagcggtt tgggaaacat caggctagga
      361 cgggatgaag atgtgccgga attcggcttc ctgtcgtggc tggcgatgct gtttgcggcc
      421 gggatgggcg tgggcctgat gtttttcggc gtggcagagc cgttgatgca ttatttttcg
      481 gacattacgg tcggcgcgcc ggaacacagg cagcagcagg cattgctgca cacggtgttc
      541 cattggggcg ttcacgcctg gtcggtgtac ggtacgattg cattggcttt ggcttatttc
      601 ggtttccgct acaaactgcc gcttgccctg cgttcttgtt tttaccccct gttgaaagaa
      661 aaaatttccg gaaggttcgg cgatgccatt gatattatgg cgttgcttgc tacttttttc
      721 ggcatcatca ccacattggg gttcggggct tcgcaactgg gcgccggatt gcaggaaatg
      781 ggctggattg ccgaaaacag cttcggcgtg caggtcttga ttatcgccgc cgtaatgtcc
      841 ctcgccgtcg tttcggcaat atccggcgtg gggaagggcg tgaaggtgtt gagcgagttg
      901 aacctgggcc ttgcgttttt gctgctgttt tttgttttgg cggcgggccc cactgtttac
      961 ctgttgtcgg cattcggcga caacataggg aactacctcg gaaatctggt gcgcctcagt
     1021 ttgaaaactt atgcgtacga acgggaacac aagccgtggt ttgaatcttg gacggtgctt
     1081 tattgggcgt ggtggtgttc ttgggcgccg tttgtgggtt tgtttatcgc gcgcatttca
     1141 aaggggcgca ccatccgcga gtttgtcttc ggggttttgc tcatccccgg cctgttcggc
     1201 gttttgtggt ttaccgtctt cggcaatacg gcgatttggc tgaatgacgg ggttgcgggg
     1261 ggaatgctcg aaaagatgac ctcctctccg gaaacgctgc ttcttaaatt ctttaattac
     1321 ctccccctgc ccgaactgac gagcatcgtc agcctgctgg tcatttccct gttttttgta
     1381 acttctgccg actccgggat ttatgtcctg aacaatatta cctctcggga caaaggcttg
     1441 agcgcgccac ggtggcaggc ggttatgtgg ggcgtgctga tgtctgccgt tgccgttttg
     1501 ctgatgcgct cgggcggact cggcaacctg cagtctatga ccctgattgt ttccctgccg
     1561 tttgccctgc tgatgctgat aatgtgtttc agcctgtgga aaggcttgag tgcggataag
     1621 aaatattttg agacccgggt caaccctacc agtgtatttt ggacgggcgg caagtggaaa
     1681 gaacggctgg tgcggataat gagccagacg caggagcagg atattttaaa attcctcaaa
     1741 cataccgcat cgcccgctat gcacgagttg caacgggagc tttcggaaga atacggcttg
     1801 agcgtccggg tcgataagat gtttcatcag gacgagcccg caatcgagtt cgtcattcgg
     1861 aaagagacga tgcgcgattt tatgtacggg attaagtctg tcgggcagga tgtatccgac
     1921 cagttgatta acgacggcaa gctgccgcat atccggcacc agacaactta caaaccctac
     1981 gcttattttt tcgacgggcg cgtcgggtac gatgtgcagt atatgaacaa ggacgagctg
     2041 attgccgaca ttttgaaaaa ctacgaacgt tatttgatgt tgttggatga tgtcggtcag
     2101 gaactgatgg cgcacgagca ggtggaattg gcagagtaag cgccttccct ccggcgtttg
     2161 ccaaaaaatg cggtgaatca gattcaccgc atttttttgc cggacggggc ttcggacggc
     2221 acggcgttca tttgtttccg tcaaactgct ccttcagcac ccgtttcaat actttgcccg
     2281 tagcgttgcg cggcagcccg tctttaaagt ggatctgttt ggggattttg aaatttgcca
     2341 gcacggtacg caggtggcgg cggatttcgt cctcgcccaa atccatacct tccttcaatt
     2401 ggacgaaggc gacgatttcc tcgtcggcat aacggtcttt cacgccgatg acggcggcgg
     2461 cttcgacggc atcgagtttg tagatttctt cttcgatctc gcgcggatag acgttttgac
     2521 ctttggaaat aatcaaatct tttttgcggt cgacgataaa gataaagccg tcctcgtcta
     2581 tggtaacgaa atcgcccgtt ttcaaccagc cgttgacgat ggtttcatcg gtggcggcag
     2641 gcatattgag gtagccccgc atcaccgaac cgcccctgac gatcagttcg cccacttcgc
     2701 cgcgcggcac ttcgaccaat tcttcatcga cggctttggc ttccaaaccg ggcaggggga
     2761 tgccgacgct gcgggctttt tgccgttcgg gcgtattgac ggcgacgacg ggcgaggctt
     2821 cgctcagtcc gtagccttcc agcaatttgg cgcgggggaa cttggcttta aaatcgagga
     2881 tggtttgttc cgccaaaggc gcgccgccgc tgataaacag gcggatgcgg ttgaaccatc
     2941 tgaaatacca agggattttc gccttgctca tcgcggtgta aatcgcgggt acgcccaaaa
     3001 acacggttgc gcgtttgagc agggcctgtt tcaaaacgtt ggaaaagggg aaaacggatt
     3061 tgaccaaaat aatcgaacac gccatataaa tcggcagcag taccatagcc gtcagcgtga
     3121 agctgtggaa catcggcagg aaaacgataa agcggtcgcg tttggaaatt ttaaagatgc
     3181 gttcgatgcc gttcaggttg gcgaacaggt tggcgtaact gattagcgcg cctttgggat
     3241 gccccgtcgt gccggaggtg tagatgatgt gtgccaaatc atttatccgg ggttggcggt
     3301 ccaagtcggg tttttcgggg aagcggcgca cgttttcaaa aaaggcatcg ccttccgccg
     3361 tttcgccggc cggccggctt ttgtccgtcc aaatgatttt ttcgacgggc gtttgcgcct
     3421 tcaggcccgc caattctttt gacaggccgg ccgaggcgaa caggaagcgc gccttgcagt
     3481 cattcaggat atacgcgtat tcgctgtttt tcaaaaatgt gttcatcggt acggcgaccg
     3541 cgccgatggc ggagacggcg aaataggcgg taataaattc tgtggaattg gaaaccgcca
     3601 gcgcgaccgt gtcgccgaac ttcacgccga tattttgcag atacgccgcg acggcttcgg
     3661 cttcctgctt gagcgcgcgg taggcggttt tttccttgcc gtcgaacact gccgtgccgt
     3721 tgccgttttt gcggcaggcg gcggcgagca tttcgtagaa attggcataa gtccggttca
     3781 tgaaacttcc tttatggttg cggacggcga acgccctcat ttgaaaacaa tatgccgtct
     3841 gaaaacggaa ggcggcagtt tatcatcttt gccttcagac ggcatatcaa acgccgcctt
     3901 tgcgccgcat gccggaggtt cgcccccgtc cttcctatgt ctgacctgaa tgccgtctga
     3961 atcaatatga agatatacgt ttcagacggc atccccctgt tgtggaaata ccgtccgccc
     4021 cccctgtccg attattgcct gattgcggcg gcgcggacga gttcttcgag gctgtcggca
     4081 agcatttccg ccgcgcgccg gtagccttgg gcggagaagt gtacgccgtc tttggcggcc
     4141 catccttggt tgagccagtt tttcatgctg catatgccgc ccattgcgtt ttgccaagac
     4201 caaaacatcg tctgcccctg acgggcgacg cgccgctgca tctgttggac ttcggtcagg
     4261 aggacggggc gcgtgccgca tacgccgagc gtgtttttca gggattcggg cgcgccgatg
     4321 atgaggatgc cggcggcggg caggctgtcg cggatttggc ggacggtatc cagccatttt
     4381 tgttcggtat cggcaatgtc gatgttgttg ttgaaggctt cgttggtgcc gtaggaaagg
     4441 ataaccaaat cggcgccggt ttgggcaagg tcgttcatac ggtcggcacg ccatttcgac
     4501 cactgggtca attgtgcgcc gttgatgccc atcgcggaaa cggtaatgcc gccggcggga
     4561 ttttcgatgt tgatgaagcc gatgtcccac ggcatttcgg tctgtatggc caggggcagt
     4621 gccgcgcccg tatccagtac ctgccagccg ccgccgttgg cggagacggt gttgccgttg
     4681 acggtcaggg tttgttcggc gagcagcggt ttggcaaaca gggaaacgcg ctgtttgccg
     4741 gttttgccgt cagacgcggt cagggtcatg ccgccgccgc tgccggtttg ggcgaggatg
     4801 ccgccgagcg ggaaatctcc ggtattgttc ctgctggtga agctttgcca gttgccgctg
     4861 tgacggacgg ccgccatgcg ctgccctttg acgttggcgg ggtaaaccca gcctatgccg
     4921 ccgtcgcccc atgttttttg caggcgtttg cgcagggcgt cggtaaagaa gtcgccggcg
     4981 gtatgcgagt cgccgatttg caggatgcgg aaggtctcgc cgctgccttg tgcgacggat
     5041 cggagttttt tcatccaagg cgaggcggcg gcgttgccgt agtcggtcag cagcccgttt
     5101 gtatcggtgt agggggcgga cggggaaacg gtaacggtgt cggggctgac ggaggcgacg
     5161 ggcagggctt ctgcctgcgt ggcggcgaac agggcggaaa atgcgatgaa gtgtttgggg
     5221 ttcatggctg tgtacttgat ggttgcgtac tcggttcaaa aacgattttt tccattattt
     5281 tttccgccag cagtttttgt ccttcggcgg taaagtgtat gccgtcctta ctgcggtagc
     5341 ggacgggttt gccgttgacg ttgacggaat cggtgtagcg gcctttcccg ccgctcagtg
     5401 tttgcgcggt gggaatcagg atgattttgc ctttcaagtg ttccgaaagc agtttgtcga
     5461 ggtagcgcat ctgaccgtcg agcttgactt ttttcatgta ggggatgccg agccagacga
     5521 cttggacgcg gtgcgtgtgt gcggcttcaa ggatgcggtc gacgcgtttc aggtattctt
     5581 gcgcccattc gtcggaagcg aatttgaggt agcgtttgcc gacggggaaa tcccacgggt
     5641 cgttcgggcc gaggaagacg gcgagtacgc tgatttcggg atgttttttc aaggtttctt
     5701 caatcgtttt cggccagtcg aagaatgagg gataggaaag ccccgtgctt tgtttgctga
     5761 ggttggcgga ttcgatgccg tattgctgtt tcaggctttt ttgcacgaag ggcgcaacgc
     5821 cctgcatcag cgaatctccg gcgaaaaaga ctttgtcgcc gctgcggatg gcggcggctt
     5881 cggtgtcttg tttccattct gttccgccgg ccggcggggc ggcttcggat gcggcggctt
     5941 caggcggcat atctgccgaa ccgccgtctt gagccgtcgg cggcgtttcg ccggacagga
     6001 aggttttgat gccgtctgaa agggcgtagg cgttttcttg caacgccgct ccgctccgcc
     6061 accatccgta ggcggcaagc ggttcgagcg ggctgttgcg gtggtaggtc tgctgccagt
     6121 aggcgttgat cggattttgg ctgaaccata cggcaatcag ggcagacatc aatatggaag
     6181 tgaaaaggga aagaaagttt ttcatgtcca aacccttaaa aattggcata aataaagccg
     6241 ggtatccccg aaggggcgag gacgataatc agcagcagga cggcggaaac ggggataaac
     6301 cacagccaca tcgggatttt ttccaaacct ttgaccgcgc cgtcgaaagc gcgttgcagg
     6361 taagggtaga gcagcatgac ggatgcaaac gaggcaagca gcagcatatc cgcccgttgc
     6421 ggcgcgttcc agccgccggc attggcaaag agggcactga aaactgcgcc tgcatcgtcg
     6481 gggtttgcgg tattgaagac gacgaaggtc aggcagacaa aatggaaggt gatgagccac
     6541 gagagcggcg caaggtattt cagacggcat agcgcgtcgc gtccgaaata ccggtcgccc
     6601 gtgttgagca gcgccagtgc cgtgccgtgc agcgcgcccc aaatgaggaa gttccagccg
     6661 tagccgtgcc agatgcctga gagtaccatt gccgccatca ggttgagctg tgtccgcaaa
     6721 aagccttttt tgctgccgcc caaggggatg tagatgtagt cgcgtatcca ggtggaaagg
     6781 ctgatgtgcc atttgtccca aaatgcgcgg atattcgcgg cacgaagcgg tgcggagaag
     6841 tttttgggca gccggaagcc cagcagcatc gccataccga taaccaaatc ggaatatccg
     6901 gaaaagtcta aaaagagttg gaaggtatag ccgtacacgc ctgccaacac gccccagccg
     6961 tcgaattggg cgggattttc aaatacgggc gacacccagt tttccgccag tatccccgcc
     7021 agccaccatt ttttggcaat gcccagtaaa atcagggaaa cggcgagggc ggggcggacg
     7081 ggcgaacgcg gtcggcgggt gcggatttgc gccagtgcac ccgcctgttc gccgtccgtg
     7141 cttttgaatg cggcggcgcg gataatcggg ccggaggtaa cggtggggaa aaaactcagg
     7201 tgcagcagca gctcgtgcca cccaaaacgc gcggcgtgcg gggcgcggaa gcagtaaacc
     7261 agataggcga cagactggaa ggtataatag gaaagcccca gcggcatcag gatgtcgatt
     7321 gcgccgcctt ttccggcata ttgtgcaatc agcggacgga aaaaatcgaa atatttgaaa
     7381 aagcccaaaa cggtaatcga ggcggcaatg ccgcacccca gccaaaagcg gcgcgtactt
     7441 tcgcgatcgg aacggagcag ttcgcccaaa aggtacacgc aggaggaata aaggacgatg
     7501 attgccgcaa atacggggct aatatggtag agccagccca taccggcagc caaaagcagc
     7561 aggttttgga cggacgggta tttcgcaaag ccccagtaaa tcggcaggaa gacgataaaa
     7621 aacagtgcga actcgacaga cagcagcggc ataaaccatc cttgggacaa aaaaataggc
     7681 gttaaaacac ggcaaagccc gttattttaa gcaggggcgg atattccttc aatcaaaatg
     7741 ccgtctgaag ggcgggaggg gcttcagacg gcatcgctcg gtcaggttcg gatatggtgg
     7801 ttcaaattca ggcagacccg caaacctgcc gccgccgtca gcatacccgc aacgccgatg
     7861 caggaaatgc ccgagtattg cgtaacctaa tgtcccagca gtgcgccgcc gcctataccg
     7921 acgttgtaaa gctccgaata aatcgagttg gcaaggtctg cggcatcgga ggcgaaatcc
     7981 aaaactttgg caaccatccc caagctgacg accacaatcg ccgtccccca tacgaacacc
     8041 aacgcgtaaa tggcggcagg aaaatgcgcc aaaggcagca aaagccccga agagagggcg
     8101 ataagcgaca ccgcgcccgc caaaaatgcg cgcggatgtt tggcaaacca tttgccgaac
     8161 aggtaagacg cggcaaaacc cgccaggccg tacagccccg gaaccaccgt aacctgccgc
     8221 gcggagaaac cgccgatttg gatgacgaag ggctcgatgt agctgtatgc ggtaaagtgc
     8281 gccgtaatca ccggcaccgt taccgcatac agcagcatca gttttttccg tttcaaaagc
     8341 aggggcaggc tgcttaaaga accggtattc acgctcggca ggcgcggcag gcttttggca
     8401 agcactgcca ttaccgccgc cgaacacagg ccgatcagca gaaaactcgc ctgccagccc
     8461 aaatattgtc cgaccatacg cccgagcgga atccccgcca ccatcgccat caccgtcccc
     8521 gtgctgagca gccccaatgc ctgattccct tttccgacaa gggcgatgcg taccgccagc
     8581 gaagccgtaa tggaccagaa caccgcgtgc gtcagcacaa tccccacgcg gcttgcgagt
     8641 aggatttcaa aacgccacga ggcaaacgac aggatgtggc tgacagtaat acagcgcaaa
     8701 caggaccagc agcaggccgc gccgttccat aattgcgcgt cagcagcatc agcggcagcg
     8761 gcagcgaagt cagcgcgaca atccacgcat aaaccgtaat catcacaccc gtttcggtcg
     8821 ccgccatgcc gaagcttcgt ccgatgtcgc tcaaaagcgc gatggggatg tattcggccg
     8881 tgttgaaaat aaatgcgccg attgccaagg caacgatgct caaccaaggt ttgtgcgaag
     8941 cgttcatctc aattccttat atgtcgcacc ccgccaaaag ggggcgcatt atggtgaatt
     9001 aacaaaaacc ggtacggcgt tgccccgcct tagctcaaag ggaacgattc cctaaggtgc
     9061 tgaagcacca agcgaaccgg ttccgtacca tttgtactgc ctgcggcccg ccgccttgtc
     9121 ctgatttttg ttaattcact atacttttct tgtctgccgg acggcaaaat ccaaatcagg
     9181 gggaagcagc cgcctcccgt tcggagaaac actcgggacg ctgctttgaa aatcgttcca
     9241 tatgttgaaa cattgccgcc tcccgattgg gcaaggttgc cattatgtcg caagcatccg
     9301 tgccgcaccg tttccgcgtt tgcccaagtt cacgctccga cttacaataa cgcccgtcag
     9361 attgcagtac caacgaaagg aaaaatcgtg gaaaccctcc tcctcgacat cggcggcatg
     9421 agctgcggcg gctgcgtcaa aagcgttacc cggatattag aaagcgtaaa aggcgtggca
     9481 agcgtcgaag tcagccttga aaacaaaagt gcgaccgtcg gatacgaccc cgcgcaaacc
     9541 gcagcggaag cactaatcga agccgtagaa gacggcggct atgatgcggc gttgaaataa
     9601 agcggcaaaa atgccgtctg aagcctttgc gtttccccga tgccgtctga agcacgctcc
     9661 gcgccttcgg gcggcatttg atttagttga acgggcagga tgaattggtt aaacttgcgg
     9721 gcaatagtgc cttcagacaa acaaaatatg ccgaacaaaa agaaaaaagc cgcctttgcg
     9781 cgctgcaacg gagaacggtg tgccgggcgt gtccgacatc aaaaattcat atgatgattt
     9841 gatgtacgaa tccaaagcat tttcccaaac cgccatcaac gccttggagg catgcgcccg
     9901 catgatgggt ttgaagccta cggcagccgc aaaagccaag gtcttggagc tgggctgttc
     9961 gatgggcaga aacatcatca acgcaggcac tttattaccc ggatgcggaa tttgtcggta
    10021 tcgacttgtc cggcaggcag gttgcgcagg gcaacgccat cattgaaaaa atgggcttgg
    10081 aaaatgtgcg tctggaagaa aaagatattt tgaccgtcga tgaatcattc gggaagttcg
    10141 actatatcat cgtccacggc atttggtcgc gggtgcccga cgcagttaaa gacaaaatct
    10201 tttcgatttg ccggaacaac ctgaccgaat acggcattgc ctatatttca tacaatgttt
    10261 accccggttg gaagcggcag gagcagttgc gcgatattat gcagtttgcc ggcagggatg
    10321 cgcttggaga acccttggaa gcaaggacgc ggaaaggctt ggatgcgatc aaggcgctgg
    10381 cggaaatttt ggaaaacgac aagggcttgg gcggcggcaa acttccggcg attcaaaaaa
    10441 tattgaatca caatacttat tatgtcgcac acgaatatat ggaaatattc aacgacccga
    10501 tttacgttaa cggtttcatt gaatgggcaa accgccaccg gctggcgtat atcggggata
    10561 ccgatttgca tgtgtctttt gtttcttgga tggcggagca tacgcgggag cggattctgg
    10621 cattggcagg gggtgattat attgccaaag aattttacag cgatatttta tccgaccgcc
    10681 aattccaccg ttcgctttta tgccgtgagg aagtcgggga tactgtcagg cgtgatgagt
    10741 cggttgccgt cgaagtgata gaaagtttga atttccgccc ggcaagaggg gaaacaatca
    10801 actttgatga aaacgatacc ctgctttcgg gcatacgcga tgtgatgaaa accggagagg
    10861 cgtttaaaac ggaagatgtt gcggaaaatc ttgcccgccg atttcccggt ctggaatttg
    10921 accgcatgaa aatcaattcc cagcttttat tgcaaaccat tctcgggcgt ttttctgttt
    10981 catcagacaa tgcgggcaaa ccattttttg aagaccataa aacctatgtg ccggcgcgct
    11041 ttacaaacta tgccgccgcc tttgtggaac acggtgcggg agcgtttgtc cggcctgcca
    11101 accgttacaa cgaaagcacg ccctcattcg gatacgggca tttgtacatt atgcggcaat
    11161 tgtcgcggcc gacgagcaaa caggcattga ttgaaacggt tgccgaaaac ttgaacatcg
    11221 tcagcgccac gcccgacggt ttgacattcc atccgcctgc cgaagtgtat gtggaagaaa
    11281 tattggcaga cttggcagac aggcattttc tcgtttcggc ggattgacgg gcaagtcccg
    11341 acggcaatcc gtccgatata ttttcagaca ggaaagacac acactgcgaa aaacggtaca
    11401 cgacacatat gaaccagaca tttaccttgc ccgatacgcg cccgtatccc caaaatccga
    11461 ttaaaaacca cctgctgctc aatgcctacc agttggcgca taattcttcc caggcttcgc
    11521 gcaaactctc gtccgggcag cttcaaaccg aaatcagggg gatgcttgag caaaaccact
    11581 atatcaacct ttccctcgcg ctgacgatgt cgcccgatgc cggaacttat gccgcgctgc
    11641 tttccagtgt gaacgcggta ctcgattgcg agaaagaagg cgaagtgcag tggttcgccc
    11701 tgccggtcgt gctggtgtca ggctgcaaga aagaacgggc aatcgagatg aagctgccga
    11761 cggaggcatt gtttgcctgc ctgcaaaact atccgcacct gcgcgcgttg acgcaagaga
    11821 cacaatggct gccttatctt gtgcattctt ccgatttgag tgcggtcgcg ccggatgagt
    11881 ggtggcgtgc caaacaaaat accgaagcgg cggcgcaaca cttacgccgt ttcgccccgc
    11941 gccctttgct gttgcccgaa gggcagtccg tccacgtcgt ttacgtgctg ggtttcggca
    12001 gcggcaaagt tcaggcggca ttggggcaga accttcttca ggcaggcttg cccttaatgc
    12061 aggtatggca ggaaaatctt gcatcggaag gcattacgct gtttgccaat ccgctttccc
    12121 ccgatacccc ggcgcgcgcg ctttcagacg gcagccacac gcgccaacgt atggcgatgg
    12181 atgtttttgc ggcaaacgcg atacgcgccg tccggatgca gggtccgcgc gtcggcgtgg
    12241 tcgctgcggc aaaggcgggc ggacagattc tattcggatt taatgcgacc gacggcgcgt
    12301 ttgaagtcgt gccgcaggtg ttttgctggc agctttcctt taccgataac attgccgtca
    12361 tccagcagaa tttcctcgac ctgatggcag aatgccgtgt agaacacgtt tacctgctgc
    12421 acaatccctt gggcgaacag gaaaacatcc cgagctatgc ggaagcgttg aaacgggaag
    12481 ggcgcaatcc gtttttcagc gcatagtgat ttgtagtagt gataggcttt ctcatttcta
    12541 taataaatca aaataaagag cacaacactt tttcattctg tgttgtgcct tgagtgaaac
    12601 gaaaggatga attataaata tgaagattgt aattgcaccg gattcgttta aggaaagctt
    12661 gacagctcaa caggtagctg aagcaataaa aagaggcttc caacaatcga tagcagatgt
    12721 ggaatgtctc ctctgtcctg tcggtgatgg gggagaaggc actgtagatg ctatccgaca
    12781 ttctcttgac ctagaagaaa aatgtctcca agtgacagga ccttttggac aaaaaaagtc
    12841 atgcgctatt ttcaaaaaga acaactagcc ctattcgaag ttgctgattt ggttggtctt
    12901 ggaaaaatcc cgctagagaa acgaaatcca ttacaaatcc aaactcgtgg tattggagaa
    12961 ttgattcgcc acctcattag tcaagaaatt aaagaaatct atattggcgt tggcggtacg
    13021 gccggtaatg atggaggtat tgggattgct gctggtttag gttatcaatt ttatgatgag
    13081 gatggaaatg ccttacccgc ttgcggtcaa tccttactaa acccagcttc tgtttcaaca
    13141 gaaaatcgct atgaaattcc tgaagatgtt cacattcgta ttttagcaga tgttgtgagt
    13201 cccttatgtg gacaccaagg tgcgacctat acgtttggca agcaaaaagg gctggattct
    13261 actatgtttg aggccgtaga tcaggcaata caagattttt atgaaaaagt cccccctgca
    13321 acattaaaac ttaaaggagc aggagctggt ggaggcatcg ctggtggttt gtgtgccttt
    13381 gctcaggcaa gtatcgtatc tggaattgac acctgcctgg acttgattga ctttgataag
    13441 aaagtgtcag atgttgactt ggttatcgtt ggtgaaggaa gactagatcg tcaaagttta
    13501 gcagggaaag cgcctattgg tgtagcaaaa agaacccctg tcggagttcc tgttgtcgct
    13561 atttgcggca gtttcgctga agatttgcct tccctaccat ttgaaaatat ccaagccgcc
    13621 ttttctattt tggagaaaag tgaaccttta gaagatagtt tgaaaaatgc cagtctttat
    13681 ttggagcata cggcttctaa tattggacat ttattaaata tgcctaagat ttagccaagc
    13741 catttcttcc agatggatgt tttggcaggt tcttccttat gcggttacga ttaaccggcc
    13801 gatgccgtct gaaagggttt ccgcttcaga cggcatcgcg ttggaacagg gatgggatat
    13861 gaaaaaaccg aaaatccttt ttgtctgcct cggcaacatc tgccgttcgc cgatggcgga
    13921 atacattttg cgccgccgtg ccgccgaagc gggcattccc ctcgaaacgg acagcgcagg
    13981 gacatcgggc tggcacgacg gcgaggatat gcaccgtgaa acggcaaaga tattgaaaaa
    14041 atacggcatt gacgcttcag gctttaccag ccgcaaaatc cgccaaagcg atgcgacggc
    14101 gtttgactac atcatcgcta tggacggcaa gaatttgtcc gaattggaaa aaactttcgg
    14161 caggcggccg gaaaaaatat tcaagctgac cgacctgata cccgaaagcg gttacgacca
    14221 tgtccccgat ccgtggtaca tgggtgattt tgaagaaaca tacaggcttg cggatgcagg
    14281 ctgtcgggca ttattggaaa agatttccaa ataaagcaat tgaatacaaa cacaaatata
    14341 aaaccgcctc ctgtcgtatc ggtcgttcgg acggcataaa cagggaattt atgaaaacaa
    14401 atttcaaaca gaaaattatc gaacaggcac gcagagagga cttgcaggta accgctttgc
    14461 gcgagcaggt attagatatt gtcttgcagc aaagcggcgt gattaaagcc tacaatgttt
    14521 tgtcacagat gcagcagcag agcgaaggcg tgcttgcacc gcctaccgcc taccgtgccc
    14581 ttgatttttg ggcggatcag ggcgttttac acaaagtggc ggcggtcaac ggctatattt
    14641 tgtgcagcca cgcgcagcac gagtgcaacg accattgcca cgaccacgaa gaagccgaag
    14701 cgcaccacag cgcgtttatt ttggtctgca ccgaatgcgg cgcggcggac gagcaaaccc
    14761 tcagccacga gtgggcggca ctgcgtgcag gcgttgccga aagcggtttt gcgctgaaag
    14821 aagaacacgt tgttttaact ggaatctgta aaaaatgtca gaagtgaaaa aaaccaaagt
    14881 ccacctgatt tcaggttttc tgggaacagg caaaaccacc gcgctcaaaa gcctgatggc
    14941 gcaaaaagac ccgaacgaaa aatgggtcat catcgtcaac gagttcggcg aaatcggcat
    15001 tgacggcgcg gtattgagcg acaacggcat ccctgtggca gaaatcgccg gcggctgttt
    15061 atgctgcacc gccggcccgc aaatgggcgt aaccgtgcag aaaatgctgc gcgacgccaa
    15121 acccgaccgc ctgatgattg aggcaagcgg actggcgcac gccgccagcg tcatcgacga
    15181 attgaaagcc aaacctctgg acagtctttt ggaaatcggc gccgtcttta ccgtcgtcga
    15241 tccgcgccag ttcatcaatc ccgattacgc gcaacaggcg ttgtataaag accaaatcgg
    15301 catttgcgac gtattggccg ccggcaaaac cgatttatgc acccccgaac agcttgccga
    15361 atttcatgac aaagcggcaa aactgttccc acccaaagcc aaagtggtcg aagtccaaaa
    15421 cgcacaactc gatatccaat ggctcgatat tcccgtagtt gaaaaatcac gctaccgcct
    15481 caaagccctg ccggacaaca ccatgggctt ccagtcgcaa ggtttcacat tccccgccgg
    15541 acgcgatttc gacggcgaaa aattgaccaa cttcttcaat gatttgccca aaatgaccga
    15601 aggactcgtc cgcgccaaag gcgtgttcca agtattggga acgtgggtgt ggctcaactg
    15661 ggtggacggg cagtggggcg cgaaccaagt gtcttggcgg cgcgattcgc gtttcgagct
    15721 gattgccaaa tcgtttgacg cggatttaat cgaacaaaaa cttaaagacg cattggaata
    15781 gcgttcacgc gctttcagac ggcatatgaa atgaaaatgc cgtctgaagg ctttaggagg
    15841 acgggatgaa acccaaattc aaaaccgttt taaccgcgct gcttttggcg gtttccctgc
    15901 cgtctatggc ggcaacccgc gtcctgatgg aaaccgatat gggcaatatc cgtttggttt
    15961 tggacgaatc caaagcctcc aaaaccgttg ccaatttcgt gcgctatgcc cgaaaaggct
    16021 tttacgacaa cacgattttc caccgcgtca tcggcggctt cgtcatccaa ggcggcggat
    16081 tgaccgagga cttggtgcaa aaggcaaccg ataaggccgt tgccaacgaa tccggcaacg
    16141 gcttgaaaaa caccgtcggc accatcgcaa tggcgcggac ggcagacccc gattccgccg
    16201 ccgcccaatt ctttatcaat ctggcggaca acggttcgct cgactacaaa aacggacaat
    16261 acggctacac cgttttcggc agggtagaaa gcggaatgga caccgtttcc aaaatcgccc
    16321 gcgtcaaaac cgccacgcgc ggcctttatc aaaacgtacc cgtacagccc gtcaaaatcc
    16381 gtcgcgttgt tgtcgggcag taacacgcag acagacgttc agacggcgtc gcccgtttcc
    16441 caaaaaacgc cgtttaaagt aaaaaaatat tttaaaacag acagttgata ttgacaaatt
    16501 caaaccgagg attttaaaat gctgccaacc caacccaacc caacccaacc caacccaacc
    16561 caacccaagc cctgatctaa gcgacacagc cggggcgaac actgaggcaa tctacacttc
    16621 agacggcatt accgccaact ctacccaact cgaacagctc aaaaaactgt ttcccgcctg
    16681 ttttgacgca gacggaaatt tccttatcga cagattacaa gccgaaattg cgccacagac
    16741 cgacatcgga cgcgaatttt acgaaatgaa ctggctgggc aaatcatatg cccgcctgct
    16801 tcgcaacctg ccgcccgaaa ccctgataag tgaagacaaa acgcacaacg ccaagcccga
    16861 aaatgcaggc agccaaaatc tgctgattcg cggcgataat ctggaagtgt tgaaacactt
    16921 aaaaaacgcc tacgcaaaca gcgtgaagat gatttacatc gacccgccct acaacaccgg
    16981 atcggacggc tttgtctatc aggacgaccg caaattcaca cccgccgaac ttgccctcct
    17041 ggcgaatatt gacgaagacg aagccgcgcg gattttagat ttcaccgaca aaggctcaaa
    17101 ctcgcacagc gcgtggctga cctttatgta tccgcgcctg tatatcgccc gcgaactgtt
    17161 aaaggacgac ggcgtgattt ttatctcgat tgacgataac gaagcggcgc agttgaaatt
    17221 gttgtgtgat gaagtgtttg gggaagggaa ttttgttgca caattgcctt ggcgaaaaag
    17281 aacagctaaa tcagatgtgc cttttggtat ttcgcaggat tatgaatgga tatttgtatt
    17341 cgcaaaatct tgccaattta ttgcagcaac taaaggcaag gaacgacgct attatgagac
    17401 tgatgatttc cccgatcgtc cttggcgtac ccacgactta acgaaacaaa caactgcggc
    17461 ggaaaggcca aatagttttt tcacaatggt tgatcccaag acaggaaaaa aatatccagc
    17521 aaatccaaat gcaacttggc gtgtaaccaa agatacattt caagattatt ataataaagg
    17581 aaaaatcgtt ttccctgatg attatgattt cttaaatatc agcaatccag ttatgcgtta
    17641 ctttaaagat gacgacatga aaaaagctgg cgaggatttt ggcaaagtag ctgtaagcag
    17701 tagattacct gaaaatgttg gtacattagc cgatgcagta gccgaatatt tggctatttt
    17761 tagtaggacg ctacctgaaa atatcggaat gactaaagaa ggcacaaaag aaatcacaga
    17821 tttatttggt agcaaaatat ttaccttccc caagcctagt caattgatta aatttttagt
    17881 ttcaataagt tcaaagagta atgacctaat cctagacttc ttcgcaggca gcggcacaac
    17941 cgcccacgcc gtgatgcagc ttaacgccga aggacaaaac ggtaaccgcc gctatatctg
    18001 tgtacagctt cccgaaaaaa ccgctgaaaa atccgaagcc cgtaaagcag gctacccgac
    18061 catcttcgac atcaccaaag cccgcataga aaaagccgcc gccaaaatcc gcgtcgaaca
    18121 tcccgattac acgggcgatt cgggcttcaa aatctttcaa acggcagaca atttcaggca
    18181 gcatccagac aaggattttt cgcccgaaca accggatttg ccgcttaacg atgaattaag
    18241 cgaagaacag ctgcaaacgc ttctgaccac ctggacgctg tatgacgggg cggcactgac
    18301 cacgccggtt gagcctgtgc ggttgggcag ttatacggcg tatctgtgcg aaaaacggct
    18361 gtatctgctt aacaagggtt ttacttccgc cgatttgctg gcgtttatca agaagctgga
    18421 cgacgatgcg gatttcaatc ccaaccgtgt gattgtattc ggcagcaata tggcaagcgc
    18481 catgcagcac gaactcgacc aggcggttcg cggttatacc aataaaaaag agattgagtt
    18541 gaatgtgatt atcagggtgt aaccggaatc cgaaagtgca ggctgctttt cggcttggat
    18601 tccccttgcg ggggaaggac gatacaacaa tgacttcatg gagaaacatt catgagcggt
    18661 tttaattacg agaaaaacca gccgcaccaa atgcgggcgg tttcggcggt tttgggcgtg
    18721 tttgacgggg caacgcccaa atatcggacg gcagacgaaa atcccgaact tttgtttgct
    18781 gcaaaacaat acgcaaacaa tatcctgaaa gtgcaaagcc aaaacggtat agacggccga
    18841 ttccccgacc gttcggacga ccaaaatatc cttgatattt ccatggaaac gggcacgggc
    18901 aaaacctata cctacacaca aaccatgttc gagctgcacc gttggctggg cgtgttcaaa
    18961 tttatcgtgg tcgtgccgac tttgtccatt aaggcgggaa cacagcagtt tttgcaaagc
    19021 aaggctttgg cagagcattt tgaacaggat ttcggcggcg attatgaagg cgtacgcctg
    19081 aaaacctatg tggtggaaag cgcgaaaaag aataagggca aaaagtccaa tgcgcccata
    19141 acgattgagc aatttgtcaa agcggaaaac aaaaaggaaa ttcatgtgct gctgattaac
    19201 gcgggcatgg ttaattcgtc gtccatgaac gatacgggcg acaaggcatt gaaggatttg
    19261 tttgacaatc ccgttgatgc attggctgcc gtgcgcccgt ttatgattgt ggacgaaccg
    19321 cataaattcc cgacccgaga tagcgcgaaa acgtggggca atatcaaacg cttaaaaccg
    19381 caatatattt tgcgctacgg tgcaacattt aacgatgaat attacaactt gctttaccgt
    19441 ttgacggcag tagacgcgtt taacgacggg ctggtcaaag gcgtgcgcgt gtttcaggaa
    19501 gaaatgcagg gcggcatgga tgcggcggta aaactggtgt cgtcggacgg caaagaagcg
    19561 aaatttgaat taaacgaaaa ggacaaaaag cagacgttca aactggcaaa aggcgaagat
    19621 ttggcgcaaa tccatccggc tatttcggat ttgaaaatcg acaaaatgaa taaaaccgtg
    19681 gtggtgttaa gcaacggctt ggagttgaaa acgggtgccg tcatcaaccc ttattcctat
    19741 tcgcaaacgg tgcaggatgc gatgatgcag cgggcggttg ccgaacattt caagctggaa
    19801 cgcgcgcttt tggcagaacg cgcgccacag cccaaaatca agccgctgac gctgtttttt
    19861 attgacgata tcgcgggcta ccgcagcggc aacgagcttt caggcagcct gaaagataaa
    19921 tttgaaagct ggattcgcgc ggaagcggca cgccgtctga aaacggaaag cgacccgttt
    19981 taccgcgatt acctgcaaaa gacgttggac gatgtatccg cctgccacgg cggctatttt
    20041 tccaaagaca atacagacag cgacgataga atcgagcagg aaatcaatga aatcctgcac
    20101 gataaggaaa aactgctgtc tttggacaac ccgcgccgct ttattttttc caaatggacg
    20161 ctgcgcgaag gctgggacaa tcccaacgtt ttccagattt gcaaactgcg ttccagcggc
    20221 agcacgactt ccaagctgca agaagtcgga cgcggcctgc gcctgccggt aaacgagctg
    20281 atggcgcggg tgcgcgatgt accgtacaaa ctgaattatt ttgtcgatag cagcgaaaaa
    20341 gactttgtga agcagcttgt cggcgaaatc aacgacaatt cttttcagga agaaatctcc
    20401 aaaaagttta ccgaagagct gaaacaaaaa atattgcaaa aataccccga tatcaaaccg
    20461 ctggtattgg taaaccaact gttttcagac ggcatcattg acgacaatga aaactttgcc
    20521 gaagacggct atgacaaatt aaaagccgcc tatcccgaag ccttccccaa aggtttggac
    20581 aaaggcaaag tcagcaacgc caaagacgag ggcaaagaca ccatcatcat gcgcgaaggc
    20641 aaatatgaag aactcaaagc cttgtgggag ctgattcacc ataaagccgt tttgcagtac
    20701 aaaatcaaag atgaagccga atttgtcgat ttatttaccg cctatttgcg tgaaaacgcc
    20761 gccaaattcc cgcaggcagg catatgcacg gcggtaaacg aagcttatat caacaacggg
    20821 cttatgcttt cccgccgcat agacagtatt gaagatgaag attttatccg tttcaacaca
    20881 atgacttacc gtgagtttct ggaaaaactg gcacaaacgg caaaaatcca gatgcagact
    20941 ttgcatcagg cgttttaccg cgtccgcgac gaactgaaca ttggcgattt tttgaatatg
    21001 cagaccatcg cccaaatcaa aaacggcttc aaccggtttt tgcttcatca ttccttccat
    21061 aaattcgaac tggattaccg gcttgtcggc agcaaaatcc atccgaccaa atttaccaat
    21121 aaagacggca aaccgcgcgc ggtgaaaaaa gcagatttgg gcagatttga agatacggag
    21181 caccggcctg ccgccggcta tctcttcggc gagattttct acgattcgga tatagaacat
    21241 gaaaatgtcg ccaacaacca aattgaaggc gtaatcgtat ttaccaaaat accgagaaac
    21301 tccatcaaaa tccctgttgc cggcggcggc acgtattcgc ccgactttgc ctatatcgtg
    21361 aaaaccaaaa gcggcgagat tctgaacttt gtgattgaag ccaaagggac tgacggggcg
    21421 gaagatttgc gaaaaagcga agagcggaaa atcaaacatg ccgaaaagct gtttgccgag
    21481 atttccaaag aaatcaaggt ggtgttcaaa acgcagtttg acggcgagag gatagccgaa
    21541 ctgatcgggc aaaatatgcc agcaggcggg cattctgaaa acgaacactg agaaacaaat
    21601 gtttggaagc cgtctgcttc gggattttaa aatgccgtct gaaacgccaa acgggcttca
    21661 gacggcattt ttgacggtgg aggtttacgc gccgcaggtt ttcggcttgt tcgccagaat
    21721 gttgatgact ttgcgttcgg ctttttgcgg ctcgattttg atttcgctct catcttcttc
    21781 gctgccgtct gaaaagcgtt cgggcatttt ttcgctgtca aaagccaaat cgccgccgtg
    21841 tttcagattt tgaccgcgtt ccaatccggc aaagtcaaag agttcggtat cggcaaggtg
    21901 ggaagggacg acgttttgca gggcggagaa catcgattcg atacggccgg ggaagcgttt
    21961 gtcccaatcg cgcagcatat cgccgatgac ttggcgttgt aggttgggct gcgagccgca
    22021 gaggttgcac gggataatcg ggaactgttt caattcggcg tatttgatta agtctttttc
    22081 tttcacatac gccagcgggc ggatgacgat gtgttcgccg ttgtcgctca ccagcttggg
    22141 cggcatggct ttgagtttgc cgccgtaaaa catatttaaa aacatggtgg cgaggatgtc
    22201 gtcgcggtgg tgtcccaagg cgattttggt gcagcccaat tcttttgcgg tgcggtagag
    22261 gatgccgcgc cgcaggcggc tgcacagcga acaagtcgtt ttgccttcgt ccaacacgcg
    22321 tttgacggtg gagtaagtgt cttcttcaac gattttgtag ggaacgccga tgctttcgag
    22381 ataggtcggc aatacttctt cggggaagcc cggctgtttt tggtcgagat tgacggcgac
    22441 cagttcaaaa tcaatcggcg cgctggcttg gagccggcgc aggatgtcta acagggcgta
    22501 gctgtctttg ccgccggaga ggcagaccat gattttgtcg cccggctcga tcatgttgaa
    22561 atcgttaatc gtgtcgccga cggcgtggcg caggcgtttg ctgagtttgt tgttttcgag
    22621 ttcttgcttg gtttttttgg acatggcggt ggtttgaaaa attagggatg cggcattgta
    22681 accgaaaaca ggggcggcaa tgccgtctga agggcttcag acggcattag agggcttatt
    22741 ttgcattttt ggttttaaag aagagatgaa ccgctttgaa gataccgccg tttgggacgg
    22801 tgagttcttt ttgcgcggcg gagaatttaa tcacggcaag ggcggtaaag ttttccgaat
    22861 cttgaacgct gtcgagtacg atgccggctt cttcgccgtc cgccgtcagc aggatgcccg
    22921 cttcggctgc cgaatttccc gacaataccg ccaagccgcg tttgacctgc ccccgatact
    22981 gggcgcgcgc gatgatttcc tgtcccgggt agcagccttt tttgaagtgt acgccgccga
    23041 tgatgtgctg gttgagcatt tgggcgacgg cggtttcttt ggtagccgcg catatccacg
    23101 gataaccgct gcggatttcg tgcagccgcc acgcgttttc ggcggcagcg tcataagggg
    23161 gtagggcggt ttcgggggcg atgtgcaaaa tgccccgatg gtgcaggatg acggaacaga
    23221 tgccgtctga gacgcattcg gaggtaaagg cgagactggg ttcttgcgcg gcaagcggtt
    23281 cggcggatgc tgccaattcc gcgccgacgg cgtaatcttc aaggatttca aaaacggctt
    23341 tggcgcgcaa cacaaacatc cgcaggcgtt tgaccgttgc ttcaagcaaa tcttgcgcca
    23401 taatcagcag caaatcgtct ccacggttga cgacaatcat attggcgatg acgcggcctt
    23461 tgggcgtgtt gtaagtcgcg taacacgcct gcccggtctg aagattattg atgtcgttgg
    23521 aaagctgtcc gtgcaggaag gtttggcggt cttcgccgct gacgcgcgcc acgccgaaaa
    23581 agggcagtaa ggttttcatc atttgcgtac tctgaaaata taaggaaatc tgtttatgca
    23641 gttgccgcgt ctctcttcac ggcggttatt ttgatttcga cgcgccactc cggacgggcg
    23701 agccgcgctt ccacgcaggc gcgggcgggc gttctgccgg cggcaaccca agcgtcccac
    23761 acgccgttca tttccgcata gtcgcccata tcacgcaggt agatgaccgc atccaaaacg
    23821 tgagccttgt ccgagccgca ttccgccagc cagcggtcgg tttgggcaag cacgtcggca
    23881 gtctgttcgg cagccgtttc accgttttcg ggaaccatgc cggagaggaa aatcaagccg
    23941 cccgcgcaaa ccgcttcgga atagcggggc gttgtgccga aataccggat atccatatcg
    24001 gtttccttcg ataaagggga tatatggtaa cattgcgctt gaccgatttc catgttttgc
    24061 atgacgaaaa atgagtaaac acacttatcc gataacacct gccgtacgcg ttttgcgtga
    24121 aaacggcatc gaatttgaac cttttaccta tgcctatgag gaacacggcg gcacggcgca
    24181 gtttgcccga ctgttcggca aagacgaaca cttggtcatt aaaaccattg ttttgcaaga
    24241 tgaaaacggt aaggggccga ttgtcttgat gcacggcgac aaacagattt caacccgcaa
    24301 tctggcgcgg catttgggtg cgaaacacat cgaacccgcc gcgcctgcgc aggcaaacaa
    24361 gtggacgggc tatctggtcg gcggcacaac gccgttcggc atccggacaa aattggatat
    24421 ttacgtcgaa cagtcggtga tggatttgga aaccatctat atcaatggcg gaaaacgcgg
    24481 gttcattatc ggtatccgcc ccgatgattt aaatattttg aacccgaaaa caatacaggc
    24541 agcggtttga ccgggaagta taaggagcaa tatggacaaa gatttgtatg ccgtattggg
    24601 cgtgtcgccg caggcggggg cggacgaaat caaacgcgcc taccgcaagc tggcgatgaa
    24661 ataccatccc gaccgcaatc cgggcaatct gcaggcggaa gaaaagttca aagaaatcca
    24721 acgggcttac gatacgcttt ccgacctgtc aaaacggacg caatacgacg cgtccttcag
    24781 acggcatgag gaacgcgggc ggcaggaaga ggcattccgc cgcgaacagg cgcgcaggga
    24841 gcagttttac ggcgaacaga tgcgccgcga acaggcgttc agacgggagt ttgaacggca
    24901 ggcttcacgt tcgcgccatg cttacgaacc gtccggcagc ggaagcgggc gcaactatat
    24961 cctcgccgcc tacatcctgt tcggtttggg tgcaatcatg cagttcatgc ccatagtcgg
    25021 cgtgattctc gcctatatga aacggaacag tttggacagc attgtctatg ccgcacatac
    25081 cgaatacctg attaaaacct tttggcgcac attttggctt tatcttttgg gcgcgctgac
    25141 tgcccgtttc ggcatcggcg tgctggttat tattgcaacg accgtctggt atttctaccg
    25201 catcatcgcc ggctttatcc gcttcaacga cggcagggcg gttgcgcccg agaaatggat
    25261 atagagcatg gcctacctgt taatcagcat cgtgttcagc gtgtcggttt ccattttgct
    25321 gaaaatggca aggaagaaaa aaatcgacat cgcgcaggcg gtcgccgtca attatgtggt
    25381 cgcggtcata ctgaccctgt cggtattgaa gccggatgtc ggcaatatcg gtgcattttt
    25441 gccgacgtgg ccgctgtttg ccgctttggg cgtgctgctg ccgtccgtat ttgtcattat
    25501 gggcaaatcc gtagaatccg ccggtatcgt caaatccgac gcggcgcagc gtttgtcgct
    25561 gtttttaccg attgttgccg ctttgacgct gtttggcgaa aaactcagtg aaggcaaact
    25621 gatcggactg tgcctcgcct ttgccgcact gttctgcctg ctttggaaaa gcgacagcgg
    25681 caaaaaatca ggcagcgcgt ggcggcaggc gttattgctg ctcggtgtgt gggcgggtta
    25741 cggcattatc gacatcctgt tcaaacagat tgccaaaagc ggcacggcat ttgcgggcaa
    25801 cctgctggtt gcatttgcgc tggcgggtgt gctgatgttt gcctgcctgt ttgccaaatc
    25861 ggtcagatgg cgcgttgaga gcgtggtcgg cggcatactc ttgggcggtt tgaattttat
    25921 gaacatcgta acctacatca ctgcgcacca aatgatgaag gacagtccca cgctggtttt
    25981 tgccggtatg aatatcggtg tgattgtttt agggacgctt tcgggcgcat tgttctttaa
    26041 ggaaaaaatc aacacaatca atacggcagg cattgtactg tcattatgct ccattgcctg
    26101 cctgttctat tggacagaag ttaaggcact gttcggcata taatccggct gattgatata
    26161 acaaaatgcc gtctgaaaca ggaaaatgct gcttcagacg gcattttgtt atacgggcag
    26221 attcaaaatt taattaataa tagttatttt taattataat ttaaatatta acaataatta
    26281 aaaatttcaa ataattctta ttatgagtgt atcttaataa aattcatttt atattcttaa
    26341 taaggaatac atgatgtccg gattctcccc caaacctaag accattatcc taagccttgc
    26401 aggtgcattt ggcgcattgg cttttgcaga caccccgaac aataccgaac agcagaaaga
    26461 actgaatacc atagtcgtcc atggcaaacg cagtgccgac caaaaaggcg cggacgacgt
    26521 gtattacaaa aacgtctcca acgcctacgt cggcaaagaa tacctcgaac gctaccgcgt
    26581 ccaatccgcc ggcgacgtac tcaaaggcct aaacggcgtg tacaacatga acacccgcac
    26641 cgccggcggc gccatcacgc ccaacatacg cggtatcacc ggcaagggcc gcatcccggt
    26701 taccatagac ggcaccgaac aaaccatcga cgtatggatg aacaactacg gcgtgggcga
    26761 ccgcaactac ctcgaccccg ccctgttccg cagcatcgcg gtggaaaaaa gccccgccct
    26821 cacgcgcggc gtgaaatcgg gcgtcggcgg cgcgatgtcc atccgcacca tcgaagccgg
    26881 cgacatcgtg cccgaagggc agaaattcgg cttccagctg aaaaccgagt ttgccaacaa
    26941 cagaacccgc ccggccaaca acctcaacca atggctgggc tgggaagact accgcaccct
    27001 gccgctcggc gccaccgccg acggcgcggg cggcggttct gacccgatca ccggacagca
    27061 gagcccgcag gcgttggtgg tagacaactt taccccgccg cagcacaaaa gcggccgcga
    27121 caactggcgc ttcggcggcg accgcagtta tatggccgcc gccgccttca aaaccgaact
    27181 ttcagacggc cttgccgcct acagctaccg caacaaaggc aactacttcg ccggtcgcaa
    27241 gggtgcggaa ggctacctga ataatccggt atacgacctg caaaaatgct acgacgaagg
    27301 cggcagcgat ttcaactgca aaaattccgc caccttcatc cccaatatgg cgcgcatcta
    27361 ccaccccggg gtggaagtgc tcaacagcaa caccgaaacc aaaaccctgc tgctgaaaaa
    27421 caactggcac ctgcccggca gccatcggct cggctggcaa tatatgcgca ccgacgtgcg
    27481 cttcggagag atcaacccct tccacaccac ctatgtgatg aatatggaag agcacaacga
    27541 gaccaaccgc cccaaagaac tgtcgccaca ggcgcagagc atcgattcca ccatccgcac
    27601 cgacacctac aaactcggct gggcctggaa gccggaaaac agccgctggg tcgatctgca
    27661 ggccaacctg tggcgcatca aaaccaacag cacccgccac cagagtggcg gcatggattt
    27721 gtcttccgcc cgccccgatc ccttctacga cgtttggtat tggtgtaccc aacgcggccg
    27781 gataccgccc gagcacgtcg acaactacag cagctgcaac gacctgatga atgatttcgg
    27841 cgtcaacggc ctgaccaaag aacaggtgtt ggccatgacc ccgaacgaca acggccaatt
    27901 ccgcgtgatt tccggcgccg agcagaaaac ccgcgtcagc cgcaccggtt tcgatatcag
    27961 caaccgcttc cgcctcagag accgcctgag catgaccttg gccgccgatt atcagaaaga
    28021 gaaactggcg gaggaagtgg aaatcgtcaa ttcgcaagac ctgttcaacc tggccggcat
    28081 ggtaaccggc ctgaccaagc tggccgggcc caggggcggc gagcggcgcg aatggggcac
    28141 caatttggtg ttcgactggc aggccgccga ccgcctgaaa atctccgccg gcatccgcta
    28201 ccacaacttc cggggcttcg ataccgccct ggccgaaggg cgtgcccgcc gcgacccgcg
    28261 ctatcaggca ggcgatggaa cccagcatta tgccgatggt gcctatctgc cgtatttcga
    28321 actggcaggc gatcaggagc ggcgtgactg gaatgtggtg gccgaacagc ttcggcaggc
    28381 gtatcagtca ggcgatgctg ccgccattgc cgcggcagta caggcagaac aggcgcacgg
    28441 cgcccgttac catttgctga aatactatga cggctcgtat cgtaccgatg gttatgtgta
    28501 tatcggcaac gatggccgtg cctattttga cggacggttc ggcggggtta acgaggcaca
    28561 gaaccaaccg ctctatcggg tgcgccccgt ttatgtgccg tttgtcaacg gcaagctgga
    28621 cagcggcgca ttgccccagc attttcatga tttcgtcagc aattatcagg aaaaagtgac
    28681 caatccgcag gggctgcacg ggacgcatta tcgatattgg gcaggcgtgg ggaatcctat
    28741 cgactgtata agaagtgatg cggcctcttg ccttgctcat aacctatcgg gaaaagtaac
    28801 caattttcaa gtcggctatc ccgagtacgg tgatgatacc ggtctcggtc tcaaaatcat
    28861 cggtcggcac tataccgagg aacagtattg ggcgcagccc aaaccgatcc gtgcccatgc
    28921 ctgggcgccg accatcgccg tcagctacga tctgaccgac aacagccgcc tgttcgtacg
    28981 ctatgcgcag atgagccgct tccccagcgt gtatgaggtg ggcagttttt ataacgacgt
    29041 ggcctatgcc ggtatgccga aggccccaaa tttccgcttc aagccggaac gcagccgcag
    29101 ctgggaaatc ggctatagct tcaattttgc cccgtattgg agccggctgc gtgccggcga
    29161 tatgcgcctg acctactacc gcaaccgtat ccaaaatgtg atcgaaacca cagattattt
    29221 ccgcaccgtg caatacgacc gcaaggacac cgccgggctg gaatggcaga gccgcatcga
    29281 caccggccgc ttctttgccg cgctcggtgc cacctaccgc ctgaaacagc agatgtgtga
    29341 ccgcgatacc gctttcgatt acgaccctta tggtgccaaa ggcgtgccga tctgcatcga
    29401 aggcggctac ggcagtacgc gcggctatca ggccttgcag ccgaaatatt caataaacct
    29461 ggatgccggg gtgcgcctgt tgggcgagaa actggagctc ggcctgcgcg gcatctacca
    29521 cagccgggtc aacaccaagc aatacgacca actgctgcaa aaggatttgg gcatcatctt
    29581 cgataccacc ggcaagcccc accattggcg gtcatcgctg acttgggatg tttatggccg
    29641 ctaccggctg cacaaaaacc tcaacgtcaa cctcggcatc accaacctga ccaaccgcta
    29701 ctatctcgac ccgatgtcca acgttgccgc ccccgggccg ggccggaccg ttacctttgg
    29761 gctgactgcc aagttctagc cgcagggcgg agtggggttt tcaggtagcc tgcgggtatg
    29821 gcgggaaggc tacctgaaaa cggattggat ataacaacaa acaattgaac actccggacg
    29881 cacaaaccca accggccccg cttatccgag gtaatggttt ttaccgtttt ttccgccggt
    29941 tcggcggtaa taaaacggtg gcttttgttt aactcttaat taaggaaacg attatgtcta
    30001 tacgtaataa aaccctgttg gccgtcttgc tgtgcggcgt ttttggtgtg gctgcggctg
    30061 ctccggctgg tggttccagc caaaggaaaa gccaagatcc aaatagtgac ccgacgacag
    30121 ttcgccccgg cacctctgac aagcaacctt tcggtgccgg taaggccggt ttggcattca
    30181 gtatcaataa cggtggaagt tggtcgaaat ccatcaatct ggatgatggt ccggtcaaat
    30241 atatccgtgc gaaatatcag gcaccgatta acggcaacga tccctatgct aatgttgatg
    30301 gtacgcgcaa tctgcaatat ctgcgcttag gcgatgtgaa accggttatc ggctggttct
    30361 acaacccaac gttgggtcag gtgtggtatg aaaaccacca caacaacact gaagtgtaca
    30421 gtgtgcgcca gatagccaac ccggcaatcc ccgccgcacc caagttcggc ggcctggtaa
    30481 tcgccaaagt gccgggcatg ggcggcagca acggcaacgt cttcttcggc gaatgggcgc
    30541 cgcgtgccgg taacccgcag caaatcagca ccgacctgaa tatggcggat ggtaaacgca
    30601 ccgtttggta tgtgggcgat aatcccaccg gcaacaccac cggtgtgaga accgccacct
    30661 acaatgtgct gggtatcaat aagcacaccc cggggcagaa cgatttctat accggtcagg
    30721 taaccgccac cttcggttct ggtataaccg ggacgatggc aggcagaatc agccgcagtt
    30781 ccgatcacgt cgattttgat cacgccaaaa tcgataacag aaatggtacc tttgccgatc
    30841 ccagtcaagg catgaccggc cgcttctacg gcaaccaggc ggcagccatg gccggctata
    30901 ccacacgtgg taccggtaat gtcggcgatg acgtagcttt cggcggtagc gccaccgata
    30961 ttgagacccg caaataacag ctgccatccg atttggtttg gcaacggcta ggctcgattt
    31021 aacccctgag gagtttcaag ctgccgtttt atgccgactg ccaagccata taaatcatcc
    31081 gtccgatacg gattgctgct cggtttcagg tagcccgtgt tcaacacagg ctacctgaaa
    31141 tttgatattt gtaaataaac atcgcctatc ccatacggtt tcggcctatc ggacattttg
    31201 ggacagcaat taaaacctgc gttactttat cttttcggaa ggcaaccatg aacaaaccac
    31261 tcagcctcgc cctcttctgc ctgcttctgc ccatcaccgc ccacgccgac aaccccgccg
    31321 acgaacgccg ccgcctgctg gacgagggca gccgccaaac gcagcaatac cgcgaaagcg
    31381 gttggctgga cacggaacag gcacgcgggg aagtagagga aaacgacggc tatatttcga
    31441 taggcggcga aatctaccaa gtcggcgata cggcggaaga actggaaagc gcaatttacc
    31501 acgccctcaa tgcgcggcag tggcacaagg tgcggcagtt cgccgcccgc tacgccaaac
    31561 tgccccggca caagcccgcg ctgattcatt tggccgatgc gctgcaaaaa cgggacgaag
    31621 gggatttccg cgcggcgggg aacagttttc agacggcctt ggaagccgaa cccgacaacc
    31681 cgcgcctgct gctcgaagcg gggcggtttt atgccgaaga caaccaaaac aaagagtctg
    31741 ccgccgcgtt tgagaaagtt ctgaaaacgg acattcccgc agaaacccgc cccatcgtgg
    31801 aaaactacct gtccgaactg ggcaaacgcc gccgctggca cggccaaatc agcctcggct
    31861 acggctataa cagcaatgta aaccaaggca acggcatcaa ccaatgcgtg tgggaaattg
    31921 cgggcatgtg cctgatggag cgcaccctgc ccgcgccaac ggattcgacc ttctcatcct
    31981 atagcgcgac cgccgaaaaa accgtcccgc tcaaagggca tcacggcgta caggtgcgcg
    32041 gtgtactgta cggcaaccgc tacacggaaa aagacaaaga ttcggcggcc atgccggatt
    32101 acggctatcg caacggttcg ctgtacgcgg gatacgccta tgccgacgca cgctcctcct
    32161 tttccctgct gccttatttc gaatacgact tccgcaaccg ccacacccac taccgcgcat
    32221 ggggtgcgga tgccgactgg tcgcgcacac tctccccgca ctggcgcatt aactcacatg
    32281 ccggagcaaa aaagaccgga tacggcggac aaagcaaaac ttattttgcc gatttcaaac
    32341 aatacgaact cggcgcgggg gcggaatttt ccatcacgcc gaaaagcggc ctgcttgtaa
    32401 atttcgatgc cgcacgcaaa gcctatcccg aaaaatcctc ttccagcaaa gaatatacgg
    32461 ctcggctggg cgcgtatagg cttttttcgg gcggtactta tttaaacgcc gtcctgctct
    32521 accgccgcag cctgtatgac gcggcaagtt tcgtcagcga taacaaacgg cggagcgaca
    32581 agcaatacat catgatggct gcggcgggtt ttccgcaatg gaatatcaaa ggcgtatatc
    32641 ccgaactgcg tttcaggcga acaatcgcac acagcaacgc ggtgtattac cgctaccgcc
    32701 aaaacgaatg gctgttgggt tttaaatatc ggttttgacc agtcgggtga taaaaaatgc
    32761 cgtctgaaag ggcgcttcct ttcagacggc atttgtccgt aatgtttaca gatgaggatt
    32821 catcaggttc tcgggggaga ggatgcggtt gagttcttct tcgctcaaca acccgcgttc
    32881 cagtacgact tcgcgcacgc ctttgccggt ctgggcgcag attttgccga ccaagtcgcc
    32941 gttgcggtgg ccgatgtacg gattcagata agtcaccagc ccgatggagt tgaaaacgta
    33001 acgctcgcag atttcgcggt tgaccgtaat gcctttgacg catttgtcgg ataggttgac
    33061 tgcggcattg cccaagaggg aaatggtttc aaacatacat tgggcgatga ccggctccat
    33121 aacgtttaat tgcagttgcc cggattcggc ggcgaaggta atcgtcgtat cgttgccgat
    33181 gactttgaag cagacttggt tgacgacttc aggaatcacg ggattgactt tggcaggcat
    33241 gatggaagaa ccggcctgca attcgggcag gttgatttct ttcaaaccgg cgcgcggacc
    33301 ggaagagaga aggcgcaaat cgttgcagat tttagagagt ttgacggccg tgcgtttcaa
    33361 tgcgccgtgt accatcacat atgcaccgca gtcggaggtc gcttcgatca ggttttcagt
    33421 cagtttgcaa ggcaagccgc tgacttcgga gagttttttg accaccagtt cggcgtagcc
    33481 tttgggcgtg ttcacgcccg tgccgattgc cgttgcgccc aaattgactt ccaagagcag
    33541 ttggcgggtg cggtcaaggt tgaggatttc ctcttccaac aatacttgga aagattggaa
    33601 ttcctggcct gccgtcatcg gtacggcatc ttgaagctgg gtgcgaccca ttttcaaaac
    33661 gtctttaaac gcttcggctt tggcggcaaa ggcgtttttc aatacggtca gtttgtcgag
    33721 caattcgccg atgctgtaat acacggcaag gcggaagccc gtgggatagg catcgttggt
    33781 ggattggctg gcgttgacgt gatccatcgg attgacgatg tcgtagcggc ctttctcata
    33841 gcccaaggct tccaatgcaa ggttggcgat gacttcgttg gtgttcatat tgaccgaagt
    33901 tcccgccccg ccctgataca cgtcggacgg gaattggtcg aggcagcggt tgttcagcag
    33961 aacttcgtcg caagcctttt caatggcggc ggcgatttcg ggttttactg cacccaattc
    34021 gccgtttgcc tgcgcggttg cttttttcac catcaccata ctgcggacaa actgcggcac
    34081 gtcggaaatt ttttgtgtgg agattttaaa gttttcaatg gcgcgcaggg tgtggatgcc
    34141 ccaatacact tcggcgggaa tctcgcggtc gcccaataaa tcgtgttcga tacggacagt
    34201 catgttttta cctttgtaag tcggataatt aatattgaaa aaatgcgcca tcggaaagat
    34261 gccgcagcag gacgaacact ataccggccg gatgaaattg tccatatcgt atgccgtctg
    34321 aaaacgggaa acgttgtttt cggtcgttac tttccggact ttccggcaag ccccgcctgc
    34381 cgccaaaata tctttcggcg ggttgtgctc cgccatatcg gcttacggtt ggcagggcgg
    34441 tttgatgaag acggcatttg tgctgattcg catcaaggct tattgttttg cagcttcgat
    34501 ttggatgtcg atgcggacgt ttttggtcat accggcgtta acgaggtagt ccacgcccca
    34561 tttggtgcgg tcgatggtgg tgctgaagtc gccgccgcaa acttcggttt ccgccatcgg
    34621 gctttggtag cagttgaatt tttcggcttt gagtttgacg ggggcggttt tgccgcgcat
    34681 ggtcaggttg ccgtcaacgg aaacaagttt tttgccgttg aagttgaatt tggtggaaac
    34741 gaagcggatg tccggatatt gagcggcatc gaagatgtcg gcggatttca ggtggccggt
    34801 gaagggttgc gaaccgcttt gcaggttggc gacgggaatg gtgatgtcga ttttgccgtc
    34861 gcgttttgct tgatcgaact cgacggaacc ggtcagaccg taaaaaccgc cgacgttggt
    34921 gctggtgttg aagtggtcga tggcgaaacg gacgttggcg tgatattcgt ccactttgta
    34981 ggtggcggag gcagtgccga cggctgccgc tgcgagcgcg gcgaagatga tttttttcat
    35041 gatgataatc ctttgtgtgg gccggtaaag gcgtttatcc taacataggc ggggatttat
    35101 ggcatttcct gccggtaaac ggtgggtttg caggcggttg acgaacgggc gggcggaaca
    35161 gggcggatga aaaagcgcgg tgttatccgt g
//
LOCUS       DAXDSD010000008        34850 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXDSD010000008 DAXDSD010000000
VERSION     DAXDSD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34850
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.008"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(320..967)
                     /gene="adk"
                     /locus_tag="ACFB2Q_000275"
     CDS             complement(320..967)
                     /gene="adk"
                     /locus_tag="ACFB2Q_000275"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC0917392.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            1182..2021
                     /gene="htpX"
                     /locus_tag="ACFB2Q_000276"
     CDS             1182..2021
                     /gene="htpX"
                     /locus_tag="ACFB2Q_000276"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC0917393.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(2257..3555)
                     /locus_tag="ACFB2Q_000277"
     CDS             complement(2257..3555)
                     /locus_tag="ACFB2Q_000277"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC0917394.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(3660..4811)
                     /locus_tag="ACFB2Q_000278"
     CDS             complement(3660..4811)
                     /locus_tag="ACFB2Q_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC0917395.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            5146..6525
                     /gene="norM"
                     /locus_tag="ACFB2Q_000279"
     CDS             5146..6525
                     /gene="norM"
                     /locus_tag="ACFB2Q_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC0917396.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHEAV"
     gene            6569..7609
                     /gene="murB"
                     /locus_tag="ACFB2Q_000280"
     CDS             6569..7609
                     /gene="murB"
                     /locus_tag="ACFB2Q_000280"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC0917397.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLANKNNASSDDIWKLAQHVCNTVFTRFQVELH
                     AEPNWLPASFSL"
     gene            7621..8271
                     /locus_tag="ACFB2Q_000281"
     CDS             7621..8271
                     /locus_tag="ACFB2Q_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC0917398.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKISNGNP"
     gene            8326..9102
                     /locus_tag="ACFB2Q_000282"
     CDS             8326..9102
                     /locus_tag="ACFB2Q_000282"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC0917399.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDTFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECNETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            9152..9718
                     /locus_tag="ACFB2Q_000283"
     CDS             9152..9718
                     /locus_tag="ACFB2Q_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC0917400.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIRKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            9738..10628
                     /locus_tag="ACFB2Q_000284"
     CDS             9738..10628
                     /locus_tag="ACFB2Q_000284"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC0917401.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            10653..11411
                     /locus_tag="ACFB2Q_000285"
     CDS             10653..11411
                     /locus_tag="ACFB2Q_000285"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC0917402.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(11466..12131)
                     /locus_tag="ACFB2Q_000286"
     CDS             complement(11466..12131)
                     /locus_tag="ACFB2Q_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC0917403.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWRFVVVQNPEIRQAIKLFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAVEKSLARYQAFQADDIKILDDSRALFDWCCRHTYIALANMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIVTKARRPLEETV
                     IWA"
     gene            complement(12328..13101)
                     /gene="folE2"
                     /locus_tag="ACFB2Q_000287"
     CDS             complement(12328..13101)
                     /gene="folE2"
                     /locus_tag="ACFB2Q_000287"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC0917404.1"
                     /translation="MNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAEGTQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTSDAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            13304..14461
                     /locus_tag="ACFB2Q_000288"
     CDS             13304..14461
                     /locus_tag="ACFB2Q_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC0917405.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(14525..15526)
                     /gene="hemB"
                     /locus_tag="ACFB2Q_000289"
     CDS             complement(14525..15526)
                     /gene="hemB"
                     /locus_tag="ACFB2Q_000289"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC0917406.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(15710..15994)
                     /gene="yhbY"
                     /locus_tag="ACFB2Q_000290"
     CDS             complement(15710..15994)
                     /gene="yhbY"
                     /locus_tag="ACFB2Q_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC0917407.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            16104..16724
                     /locus_tag="ACFB2Q_000291"
     CDS             16104..16724
                     /locus_tag="ACFB2Q_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC0917408.1"
                     /translation="MAVRSKSSKAWLHEHINDHYVHMAQKDGYRARAAYKLLEINEKD
                     KIIKPGTVLADLGSAPGSWSQVAAKLTGTSGTVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            16788..18755
                     /gene="ftsH"
                     /locus_tag="ACFB2Q_000292"
     CDS             16788..18755
                     /gene="ftsH"
                     /locus_tag="ACFB2Q_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC0917409.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            18977..19408
                     /locus_tag="ACFB2Q_000293"
     CDS             18977..19408
                     /locus_tag="ACFB2Q_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC0917410.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            19401..19679
                     /locus_tag="ACFB2Q_000294"
     CDS             19401..19679
                     /locus_tag="ACFB2Q_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC0917411.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            19732..20310
                     /gene="pth"
                     /locus_tag="ACFB2Q_000295"
     CDS             19732..20310
                     /gene="pth"
                     /locus_tag="ACFB2Q_000295"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC0917412.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDIISGKWEEATRFLHSK"
     gene            20371..20577
                     /locus_tag="ACFB2Q_000296"
     CDS             20371..20577
                     /locus_tag="ACFB2Q_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC0917413.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            20574..20936
                     /locus_tag="ACFB2Q_000297"
     CDS             20574..20936
                     /locus_tag="ACFB2Q_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC0917414.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFAVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            21165..22580
                     /locus_tag="ACFB2Q_000298"
     CDS             21165..22580
                     /locus_tag="ACFB2Q_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC0917415.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            22700..23209
                     /locus_tag="ACFB2Q_000299"
     CDS             22700..23209
                     /locus_tag="ACFB2Q_000299"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC0917416.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            23377..24759
                     /locus_tag="ACFB2Q_000300"
     CDS             23377..24759
                     /locus_tag="ACFB2Q_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC0917417.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIAIGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVTTPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(24815..25570)
                     /locus_tag="ACFB2Q_000301"
     CDS             complement(24815..25570)
                     /locus_tag="ACFB2Q_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC0917418.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMDG
                     GVIVEQGSPKELFDHLKHERTRRFLSQIQSAKI"
     gene            complement(25580..26296)
                     /locus_tag="ACFB2Q_000302"
     CDS             complement(25580..26296)
                     /locus_tag="ACFB2Q_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC0917419.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(26286..27113)
                     /locus_tag="ACFB2Q_000303"
     CDS             complement(26286..27113)
                     /locus_tag="ACFB2Q_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC0917420.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVARNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAQLVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            27398..28306
                     /locus_tag="ACFB2Q_000304"
     CDS             27398..28306
                     /locus_tag="ACFB2Q_000304"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC0917421.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            28383..31985
                     /gene="recB"
                     /locus_tag="ACFB2Q_000305"
     CDS             28383..31985
                     /gene="recB"
                     /locus_tag="ACFB2Q_000305"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC0917422.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIAHALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRQTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            32024..32383
                     /locus_tag="ACFB2Q_000306"
     CDS             32024..32383
                     /locus_tag="ACFB2Q_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC0917423.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            32508..32987
                     /locus_tag="ACFB2Q_000307"
     CDS             32508..32987
                     /locus_tag="ACFB2Q_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC0917424.1"
                     /translation="MKHILPLIAASALCISTASAHPADKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(33262..34641)
                     /gene="radA"
                     /locus_tag="ACFB2Q_000308"
     CDS             complement(33262..34641)
                     /gene="radA"
                     /locus_tag="ACFB2Q_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC0917425.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADASTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
BASE COUNT         8277 a        10348 c         8786 g         7439 t
ORIGIN      
        1 caacgtaaat tcaaaccgta ggccgggcat tgatgtccga cctacgcgga ctcaagcccc
       61 cgacaaactg tttcggataa atttgaaccg gtacatcagc cgtcattccc gcgcgggcgg
      121 gaatccattg ataaaactca gtttatcaga tttaaacagc gattgcccaa attcagaaat
      181 ggattcccgc ctgcgcggga atgacgccga atgattattt ttcggttgca acacgctgtt
      241 tatcaaaaac aggtcgtctg aaaacctgat tttctgtttt cagagtgcga agcctgcccg
      301 tgggtacgac cttttcgatt tatttgccca atgcgcccaa tacttcggct ttcacagctt
      361 ctactgcttg ggtaccgtcg actttgatgt atttcggcgc gtgttcgcct tccagcttgc
      421 tgtaaaaatc aaccaaaact tcggtttgct cgtggtaaac ggcaagacgt tttttcacgg
      481 tttcttcttt gtcgtcgtcg cgctgaatca aatcttcgcc ggttacgtcg tctttgcctt
      541 cggttttagg cggattgtag gtaacgtggt aagtacggcc ggaagccaaa tgcacgcggc
      601 ggccgctcat gcggtcgaca atcacgctgt cggatacgtc gatttcaacg accgcgtcca
      661 aacccacgcc tgcttccacc atcgcttcgg cttgcgccaa tgtacgcggg aaaccgtcga
      721 acaggaaacc gtttttgcag tcgtcttgcg cgatgcgttc tttgaccatg ccgataatga
      781 tgtcgtcgcg caccaaaccg ccttcatcga tgattttttt cgcttccaaa cccaagggcg
      841 tgcctgcctt aatcgcggca cggagcatgt cgccggtaga gatttgcgga atgccgaacg
      901 cggcggtgat gaattgcgcc tgagtgcctt tgcccgcgcc cggtgcgcct aaaagtaatg
      961 ctttcatgaa gtgatccttt taaatgtgtt tattggaatc aaacagatgg tttacgattc
     1021 tacacggctt ggcgcaatag ttcaaaaacc aactgcacaa actgccattt gcaagcaaat
     1081 gttttgcata cctttgccga gcctgatatt cacaaggcgg caactttttg ggacaatccc
     1141 caaagtttca aaacatcaca ccctaccaaa aaggaacatc cgtgaaacgc atttttctgt
     1201 ttttggctac caatatcgct gttttggtcg taatcaacat tgttttggcg gttctgggca
     1261 tcaacagccg gggcggcgcg ggcagcctgt tggcgtattc cgccgtcgtc ggcttcactg
     1321 gttcgattat ttcgctgctg atgtccaaat ttatcgccaa acaatcggtc ggtgcggaag
     1381 tcatcgacac gccgcgcacc gaagaagaag cctggcttct gaacactgtc gaagcccaag
     1441 cgcggcaatg gaacctgaaa acgccagaag tcgccatcta ccactccccc gaacccaatg
     1501 cctttgccac gggcgcatcg agaaacagct ccctgatcgc cgtcagcacc ggtttgctcg
     1561 accatatgac gcgcgacgaa gtggaagccg tgttggcgca cgaaatggcg cacgtcggca
     1621 acggcgacat ggttacgctg acgctgattc aaggcgtggt caataccttt gtcgtgttcc
     1681 tgtcgcgcat tattgccaac ctgattgccc gaaacaacga cggcagccag tcccagggaa
     1741 cttatttcct agtcagcatg gtattccaaa tcctgttcgg cttccttgcc agcctgattg
     1801 tcatgtggtt cagccgccaa cgcgaatacc gcgccgacgc gggcgcggca aaactggtcg
     1861 gcgcaccgaa aatgatttcc gccctgcaaa ggcttaaagg caacccggtc gatttgcccg
     1921 aagaaatgaa cgcaatgggc atcgccggag atacgcgcga ctccctgctc agcacccacc
     1981 cttcgctgga caaccgaatc gcccgcctca aatcgcttta aaccgatttg aaacgacaaa
     2041 aaaccgtacg cacaagcagt acggtttttt cgtatatgcc gactgaaaac atcaggtttc
     2101 cataccggca ccaaccgtat ccatttccgc accggatacg ccgcattgat atatccccga
     2161 caaaccgtgt ccggacagaa aatatcagat aaaacagcgg taaaaatgcc gtctgaaacc
     2221 aattagggct tcagacggca tttttactgc aaaaccttat gcgaacggat gatgcagcac
     2281 aatcgtttct tcgcggtcgg ggccggtgga gacgatggcg accggcgcgc cgcagacttc
     2341 ttcaatccgt ttcaaatatg ctttggcatt ggcaggcaat gcgtcgtagc ttttcacgcc
     2401 gacagtcgat tcgcgccagc cgggcatggt ttcgtaaatc ggtttgcagg tttccaccgc
     2461 atccgaaccg caaggcagga tgtcggtttt gccgccgcct ggcaattcgt agccgacgca
     2521 gatattgatg gtttcaacgc cgtccattac atcgagtttg gtaatgcaca taccggaaat
     2581 gccgttgatt tggatggagc gtttcagggc ggcggcatca aaccagccgc agcggcgcgc
     2641 gcggccggtt accgaaccga attcgtgtcc gcgctccgcc aaacctgcgc ctacttcgtc
     2701 gaacaattcg gtcgggaacg gacccgaacc gacgcgcgtg gtataggctt tgacgatgcc
     2761 caaaacataa tccagcattt gaggacctac gcccgcgcct gccgaagccg cgccggcgag
     2821 acagttggac gaggtaacga aggggtaagt gccgtagtcg atgtccaaca acgcaccttg
     2881 cgcgccttca aacagcagtt tttcgccgtt tttgtttttt tcgttcaaca cgcgggacac
     2941 gtcggcaatc atcggcgcaa tgcgcggcgc gactttttcg ataaccgcca tcacgtcttc
     3001 cgctttaacc ggtccggcgt tgtgcaggta ttggagttgg acgttgtaat aggcaaggac
     3061 ggcatccagt ttttcacgca gtttttcagg atgcagcaaa tcggcggcgc gaatggcgcg
     3121 gcgtgccact ttgtcttcgt aggcagggcc gatgccgcgg ccggtcgtgc cgattttgcc
     3181 tttgccgcgc gatgcttcgc gggcttggtc gagcgcgatg tggtaaggca ggatcagcgg
     3241 gcaggtcggc gcgattttca gacggccttc gacgtttttc acgcctgccg cgttcaactc
     3301 gtcaatttcg cccaacaggg cttcggggga aacgacaacg cccgaaccga tgaagcaatc
     3361 caatccttcg tgcaggatac cgctcggaat caggcgcaaa atggtttttt tgccgccgac
     3421 gaccaaggta tggcccgcat tgtggccgcc ttggaagcgc acgacgccgc cggcttcttc
     3481 cgccagccag tcaacgattt tacctttacc ctcgtcgccc cactgtgcgc cgattactac
     3541 aacattttta gccatagcca tataacctat cgatattaaa aattattgcg gaaaatcccc
     3601 aaaccggtgc ggctttgcgc cccgccccgg tttgcctttc atccgctatg aacgggtatt
     3661 tagcgtttca cgacctgcca aacgccgtcc gtctttttca gacggcctgc aagctcttcc
     3721 gaaacattgt gtccgatacc gtagtcaatc acgacacact gcccttgttc acgcaaggct
     3781 tcgaccgctt cgcgcgccgc ttctgcatct tccgcatcga ccaacacggc gggctgccgt
     3841 tcgacggcgg gcaaacgccc gataaagctg cgtaagtcga aactgaatcc cgttgccggg
     3901 cgcgccctgc cgaaatatcc gcccaatccg tcataacgcc cgccgcgcgc gaccgcgtcg
     3961 tggaaatcgg cggcataggc ggcatacagc aagcccgtgt ggtaattgtc gacgcgcagc
     4021 tcggacaagt cgatatggat ttcattatcg gggaatgcgt cgcacaccgc ctgcaattcg
     4081 tccaacgcgc cgccgaccgc cgacaaatcc ggcaaccgtc cgcgcgcgtc ggacaacact
     4141 tcacgcccgc cgtacaggcg cggcagcaac gagaatgctt ttgcccacat accgtccagc
     4201 ttccaagcct tgacctgcgc ttcgaccgac ccggtatctt tatcctgcat caaggcaagc
     4261 agcgttgcgg actgccccgc atccaaatgc gccgcatcgg acaaggcgcg gaatatgccg
     4321 atatgcccca gcgaaagcag cactttgccc atatcggcaa ttttcatgct tttcagcata
     4381 aggtctatca gctcgatgtc gccacggatg tcggcaaaac cgtacatttc cgcccctgcc
     4441 tgcaagggtt cgcgcatatt cggcagaccg tcgggctgcg cgtgcaacac cggaccggca
     4501 taacacaacc ggttaatccc ttggttggcg gacaaaagat gggcatcgat acgcgccacc
     4561 tgcggcgtga tgtcggcgcg tatgcccaac tgcctgccgc tgagcctgtc cgttaccaaa
     4621 atggttttca gtgaaagccc cgcatcgata tgcgtcagca gggaatgtgc gtactccatc
     4681 agcggaggct gtaccagttc ataaccgtgt acgcggaaca gtgccaacaa ctgctcgcgc
     4741 gcgctttcaa gctgccgcgc gttcgtgggc agtacgtcgg cgatatgttc cggaagctgc
     4801 catgtctgca tgagaatctg ccttctgttt tatcctgtaa acacaaaaga ctgcccgatc
     4861 cgcaatcaga cggcatcttt gccgcaacgc atcacgccgc tgtcaaaacg gcgatgcttc
     4921 cttccgattc agaatccgaa cagcccctgt ccgacggctc aatgccgtca agtcgttaaa
     4981 accaaacttt accataaaat acacacaatc tgaatatact tttatacccc gtatccgccg
     5041 tctgacggca cggtattttt tactatataa tgtgcaccat tatttctcat cacttcccgc
     5101 tgccgttttt ttgtttcaga cggcattttt attgactgaa cacttatgct gctcgacctc
     5161 gaccgctttt ccttttccgt cttcctgaaa gaaatccgcc tgctgaccgc ccttgccctg
     5221 cccatgctgt tggcgcaggt cgcgcaggtg ggcatcggtt tcgtcgatac cgtgatggcg
     5281 ggcggtgcgg gcaaggaaga tttggcggcg gtggctttgg gcagcagcgc gtttgccacg
     5341 gtttatatta cctttatggg cattatggcg gcgctgaacc cgatgattgc ccagctttac
     5401 ggcgcgggta aaaccggtga agcaggcgaa acggggcggc aggggatttg gttcgggctg
     5461 attttgggga ttttcggcat gattttgatg tgggcggcga ttacgccgtt ccgcaactgg
     5521 ctgactttga gcgattatgt ggaaggcaca atggcgcagt atatgctgtt caccagcttg
     5581 gcgatgccgg cggcaatggt acaccgcgca ctgcacgcct acgcttccag cctgaaccgc
     5641 ccgcgcctga ttatgttggt cagctttgcg gcgtttgtgt tgaacgtgcc gctgaactat
     5701 attttcgttt acggcaaatt cggtatgccc gctttgggtg gcgcaggttg cggcgtggcg
     5761 acaatggcgg tgttttggtt cagcgcgctg gcattgtgga tttatatcgc caaggaaaaa
     5821 ttcttccgcc cgttcggact gacagcgaaa ttcggcaaac cggattgggc ggtgttcaaa
     5881 cagatttgga aaatcggcgc gcccatcggg ctgtcttatt ttttggaagc cagcgcgttt
     5941 tcgtttatcg tgtttttgat tgcgcctttc ggcgaggatt atgtggcggc gcagcaggtc
     6001 ggcatcagtt tgtcggggat tctctatatg attccgcaaa gcgtcggctc ggcagggacg
     6061 gtgcgcatcg gcttttcgct tgggcggcgc gaattttcgc gggcgcgtta tatttcagga
     6121 gtatcgctgg tgtcgggctg ggtgctcgcc gtgattaccg tgctttcctt ggtattattc
     6181 cgttcgccgc tggcaagcat gtacaacgat gatccggcag ttttaagcat tgcctccacc
     6241 gtcctgctgt tcgccggcct gttccaaccg gcagacttta cccaatgtat cgcgtcctat
     6301 gccctgcgcg gctacaaagt caccaaggtg ccgatgttca tccacgccgc cgccttctgg
     6361 ggctgcggcc tgctgccggg ctatctgctc gcctaccgtt tcgatatggg catttacggc
     6421 ttctggacgg cattgattgc ctcgctcacc atcgcagccg tcgccttggt gtggtgcttg
     6481 gaaaaataca gtatggagtt ggtcaaatca cacgaggccg tctgaaaacg cagcacactt
     6541 caattcaaat acagttaaag ctcaaaccat gcaacccatc cgataccgaa ccgacctcac
     6601 cccctacaac actttcggcc tgcacgccca agcccgggcc tttatcgcgc ttaaacacgc
     6661 cgacgaattg cgcgacatcg tccgactgcc cgagttcgac cgcgatactg ttttatggct
     6721 gggcggcggc agcaacatcc ttttgatgca ggattacgac ggcttggtcg tccatatgga
     6781 aaacaagggc atacgcgaga ttgcgcgctc agacggcatg gttctgattg aagcgcaggc
     6841 gggcgaaatt tggcacgatt tcgtgttgca caccgtcgcg ctgggtttga gcggtttgga
     6901 aaacctgagc ctgattccgg gtacggtcgg cgcgtcgccc gtgcagaaca tcggcgcata
     6961 cggcgtggag gcgaaagatg tgattcacag cgtgcgctgc ttcgatttgg atacggaaac
     7021 ctttgtaacc ctttccaatg ccgactgccg cttcgcctac cgcgaaagcc tgttcaagca
     7081 ggaaggcaaa gggcgttatg tgattgtttc ggtcgtattt gcattaaaaa cgcattttgt
     7141 gccgaacttg ggctacggcg atttggcggc gaaggttgcc gaattgagtg caggtaggga
     7201 ggcgacggcg aaagacgttt ccgatgcggt atccgccatc cgcaacagca aattacccga
     7261 cccgaaagtt ttgggcaatg tcggcagttt ctttaaaaac cccgtcgtca gcgcggaaaa
     7321 agccgccacc ctgttgcagc ggcatcccga tatgccgcgc tatccgcagc ccgacggttc
     7381 ggtcaaactc gccgccggct ggctgatcga ccaatgccgt ctgaagggct tccaaatcgg
     7441 cggtgcggcg gtacatgacc ggcaggctct ggttttggcg aacaaaaaca atgcctcttc
     7501 agacgacatc tggaaactgg cgcaacacgt ctgcaataca gtatttactc gatttcaggt
     7561 agaattacac gccgaaccca attggctgcc cgcttcgttc agcctgtaaa tccaaggagt
     7621 atgcccgtga cccgcattgc aaaaaccaat acttacaccc gcatcatcga cgccagcctt
     7681 gcgcttttca acgaggaagg cgagcgcaac atcagcacca accatattgc cgcccacttg
     7741 ggcatcagtc cgggcaatct ctattaccac ttccgcaaca aagacgaaat catcgtccaa
     7801 ctgttcaaac gttacagcga agccctgctg gaatacctca acgaagccgt gttgccctct
     7861 gatgtggaag actctatcaa ctatatggca ggcatttacg atgtcatgtg ggaataccgc
     7921 ttcctcttta gcgacgtaaa caccctactt gcgcgcagtg ccgaattgtt gggcgaacac
     7981 aataccttta cccaagccaa agtctcgccg cttctagtca aactgctgac ccagctcaac
     8041 gggctgaacg tcatcaacgc cgaccaaacc gctatgaacg acctcgccgt caatatgtgg
     8101 atggttacga aatactggtt cgacttcgac agttccctgc gcggccgtac caagctgacc
     8161 gaagactcca aagcacgcgg cgtccgtcgt accttaagcc tccttcgccc ctatcttttg
     8221 cccgaacacc gcgcggaata cgaccgcaaa atcagcaacg gcaacccgta aacccataac
     8281 gtccgaatca gccttcagac ggcacttacc cgacggtatc agcgaatgcg cgcagtcccc
     8341 ccgccccata tctccatcac aggcctaggt tacctcggcc tgccgctggc acaaaagttt
     8401 taccgacacg gcagccgcgt cgccgccata aaacgcagcc tgacttcgga cgacatcaac
     8461 ctgcccatac acctcgacac cttcgacctc aaccgtaccg atacctttca gacggcattg
     8521 tggcggcatc acgccgacaa acccgtctgg ttcttcctgc tgccgccctc ctttttggcg
     8581 cattacgccg acaccgtcaa acaatgggca gagcttgccc gggcgtgcaa cgtgcaacac
     8641 ctgattttca caagcagcac aagcgtttac ggcgataaag cgcgagaatg caacgaaacc
     8701 gccgcacccg atccgcaaac cgagtccgcc cgacaaatcc ttgccgccga acaacacctg
     8761 ctcgacaacg gcgttccgaa catcgacatc ctgcggctgg gcgggcttta ttgcgccgaa
     8821 cgccatcccg tcagccgcct cgtgcaaaag caaaacatcc aaggcggcaa ccggcctgtc
     8881 aatatcgtcc accgcgacat cgccgtcgaa aacctgtttc agacggcatc caaccccggc
     8941 ggcaggcggc tgaaaaacat tatcgaaccg cgccacccga cacgccgcga attttatacg
     9001 gaagaagccg ccaaactcgg cctgcccgcg ccggatttct cacccgacga cagcgtgggc
     9061 aaaatcatcc gtaccgtttg cgataacggc ttaagcctgt aaaataagcg gcaacagcaa
     9121 caacaggcat tctccgcccc aatcagaaaa tatgtccgcc ctcctcccca tcatcaaccg
     9181 cctgattctg caaagcccgg acagccgctc ggaacttacc tcctttgcag gcaaaacact
     9241 gaccctgaac attgccgggc tgaaactggc gggacgcatc acagaagacg gtttgctctc
     9301 ggcgggaaac ggctttgcag acaccgaaat taccttccgc aacagcgcga tacggaaaat
     9361 cctccaaggc ggcgaacccg gggctggcga catcaggctc gaaggcgacc tcatcctcgg
     9421 catcgcggta ctgtccctgc tcggcagcct gcgttcccgc gcatcggacg aattggcacg
     9481 gattttcggc acgcaggcag gcatcggcag ccgtgccacc gacatcggac acggcatcaa
     9541 acaaatcggc aggaacatcg ccgaacaaat cggcggattt tcccgcgaac ccgagtccgc
     9601 aaacaccggc aacgaagccc ttgccgactg cctcgacgaa ataagcagac tgcgcgacgg
     9661 cgtggaacgc ctcaacgaac gcctcgacag gctcgaacgc gacatttgga tagactaacc
     9721 ttcagacggc atccgacatg aacagccctt ttcacaacat cggtatcgta acccgcccca
     9781 acacgcccga catccaagat accgcacaca cgctgattac ctttttgaag cagcacggct
     9841 ttaccgtcta tctcgacgaa gtcggcgtaa gggaatgctg catctatacc caagacaccg
     9901 acggctgcca tatcgtcaac aagaccgaac tggggcaata ctgcgacctg gtcgccgtct
     9961 taggcggaga cggcaccttt ctctctgccg cccgcgaaat taccccccgc gccgttccga
    10021 ttatcggcat caaccaaggt catttgggct ttcttaccca aattccccgc gaatatatga
    10081 cggacaagct attgcccgtt ttagaaggaa aataccttgc cgaagagcgc atcctgattg
    10141 aagccgcact gatccgcgaa ggcaaaaccg ccgaacgcgc cctcgccctc aacgatgccg
    10201 tcctctcccg cggcggtgcc ggacagatga ttgaatttga agtcttcgtc aatcaggaat
    10261 tcgtctatac acaacgttcg gacgggctga ttgtctccac ccccaccgga tcgaccgcct
    10321 attcgcttgc cgccggcggc ccaatcatgc aggcaggatt acacgccttc acgctcgtcc
    10381 ccatctgccc gcaatctatg actaaccgcc ccatcgccat tccagacacg tccgaaatcg
    10441 aaatcctcgt tacccaaggc ggcgacgcgc gcgttcattt cgacggacaa tccttcatcg
    10501 acgtgcaaaa cctcgaccgc atcatcatcc gccgctacca caatccctta cgcatcctac
    10561 acccgaccga ctaccaatat ttcaaaaccc tgcgccaaaa actgcactgg ggcgagcaat
    10621 tagtctaagc cggccttcac ccgaaagtac ccatgaattc caaaatttcc tccgaccata
    10681 cagaagatac catacgcctc tccaaacgta tggcacaact ggggctttgt tcgcgccgcg
    10741 aagccgacgg ccatatcgaa cagggctggg taacggtcaa cggcaaaacc gccgtactcg
    10801 gtcagaaagt ttcaccggca gaccgtatcg aactgaacaa gaaagcccac gaacagcagg
    10861 cggcacgcat taccatcctg ctgaacaaac ccgtcggcta tgtcagcgca caagcggaaa
    10921 aaggctataa atccgccgcc gaactgatta cccctgaaaa ttgctgggaa ggcgacacca
    10981 gccgcatcag tttcgatccg aaacacaaaa tcggtcttgc ccccgccggc aggctcgata
    11041 tcgattcagt cggattgctg gtgttgacgc aagacggccg tatcgccaag cagcttatcg
    11101 gcgaaaacag cggcagtgaa aaagaatatt tggtgcgcgt gcgcggcaaa ttggacgaaa
    11161 aaggacttgc cttactgaat cacggattga gtttggacgg tgataaactg cgtcccgcca
    11221 aagtagaatg gcaaaatgaa gaccaactgc gcttcgtgtt gaaacagggt aaaaagcggc
    11281 aaatccgccg tatgtgcgaa ctggtcggac tgcgcgtcgt cgggctgaaa cgcatccgca
    11341 tgggcaaggt caaactcggc aggctgccgc ccggcaaatg gcgttatctc gctcccggcg
    11401 aatcgtttta aataagcatt aaaaaatgcc gtctgaaaat ccttgcgttt tcagacggca
    11461 ctgccttatg cccaaataac ggtttcttcc aagggcctgc gcgctttcgt gacgatttcc
    11521 tgaacccggt agccgaatgt cgcggcaacg gacacgcccc attctgccgc atcgaacaaa
    11581 ccgaactgcc cggacaatac gcgttccata tcggcatagt tgaaaccttc caccgggcag
    11641 gaatcgatac ccgccatcgc cgcacccgtc atcatgttgg ctaacgcgat ataggtgtgg
    11701 cggcagcacc agtcaaacaa ggcgcgcgaa tcgtccaaaa tcttgatgtc gtcagcttga
    11761 aacgcctgat acctcgccaa agatttttct acggcatccg gttcggtaac gccgcgccgt
    11821 ttgagacttt ccaacataaa cgggctgtcg aagcgggcat ttttcttcgc caaaaacacc
    11881 accaaatgac tggcggtatc caaagcatcc gccatacccc aagaaaacag cttgattgcc
    11941 tgtcggattt cggggttttg aaccacaaca aaccgccaag gctccgaacc gaccgaactg
    12001 ggcgacaaac gcccgagttc taaaataaac tgaaaatcct cggcactgat tttacgtgcc
    12061 gcatcgtaat gccggcacga tttgcggttt ttaaatgcgg atagaacctg ctctttgctt
    12121 aatactgtca tcgtgcatcc ttttattact ttgattgcaa attgatttca aaccgatacg
    12181 gcactgccgc accctataag ccgtccccgc catcaagctt cacccggatt ttacccatct
    12241 ctctacggct gggaaaacaa agcggcggca gattcgggaa taactcccca atgcctttca
    12301 gacggcattt ggacatcgca aacgcgccta cgggtaggcg atataggcat aagccgaatg
    12361 gttgtggata gactcgaaat tctcgctctc gacgacgaaa ctcttgatgc gtttgtcggc
    12421 aatcagcgaa gtagcgacat cgcgcaccat atcttccacg aatttcgggt tttcgtaggc
    12481 cttttcggta acgtattttt catcggggcg tttgagcagg ccgtagagtt ggcagctcgc
    12541 ctgcgtttcc acataatcga taacttcctc gataccgact tcggcatcgg aagtcaggct
    12601 gacggtaacg tgcgaacgct gattatgcgc gccatattgg gaaatttctt tggaacacgg
    12661 gcaaagcgag gttacgggaa tcatgacctt catactgtgg ccgtatgccc cgtctttcat
    12721 ttcacccgtg aggctgacat cataatccag taaggaccgg ataccggata ccggcgcggt
    12781 tttcttgcgg aaaaacggaa aagaaacgct gattttgccg gcgcgggaat ccaaaagcgc
    12841 gaccatttcg gcagtcagcc tatgcaattg tgcgaaatcc aagacttcgg tatgttgctc
    12901 catcaatgcg acaaagcgcg acatatgcgt ccctttctgc tcggcgggca gataaaccgt
    12961 catcgtcagg cgagcaaccg tggattgtgt tccttcggcg gttttcaggg taatcgggaa
    13021 gcgcaggtct ttaatgccga cctgattgat cggcaggttg cgcaaatctc tgctggattg
    13081 cacgtctgca atggcgttca tgcgttgttt gtccttaata ttcagataat tattgagatg
    13141 tgtgtattgt atggcaggca gatgccgtct gaaaaaacgc tgtcggccgc ctgcctgcaa
    13201 atgcgagatt atatcacttg cttttggcgg ctgcattgat tgtttctatt ttcgggatag
    13261 tgataaaatc ccgacttatc catatcttac aaggcagaaa cagatgaaat tcccgacccg
    13321 tgccatccat tccggctacg attgcgacga acacaaccgc gcgctgatgc cgccgattta
    13381 tcaaaacagt atgtttgcgc tgcacgaaat cggcgaaaat gtgccttacc gttattcgcg
    13441 cctgagcaac ccgacccgtc agattttgga agacaccgtt gccgatttgg aacacggtgc
    13501 ggcaggtttt gcgttttcca gcggtatggc ggggattgat gccgtgtggc gtaccttcct
    13561 gcgcccgggc gataccatcg tcgcggtcgc cgatatttac ggcggcgcat atgacttatt
    13621 ggtcgatgtt tatcaaaaat ggggggtgaa cgttgttttt gccgatttgg gcaatcccga
    13681 taatttggac gaattgctca aagcgcacaa tgtcaaactg gtttggctgg aaatgccgtc
    13741 caatccgctt ttacgcttgg tagacatcaa agcccttgca gcgaaagcca aagcagccga
    13801 cgcgctggtc ggcatcgaca acacctttgc cacgccgtat ctgcaacagc ccttggatat
    13861 gggttgcgat tttgcattcc attccgctac caaatatttg tgcggccatt ccgacgtatt
    13921 gatgggcatc gtcgttgcca aaaccaaaga gctggctcag cctttgcacg atatgatggt
    13981 gcataccggc gcgattgcag gcccgacgga ctgctggctg gtgttgcgcg gcatcaaaac
    14041 gcttgccctg cgtatggagg cacactgcaa aaacgcactc gaaattgcgc gccgtttgga
    14101 agcccatcct gccattgaaa aagtgttcca tcccggcctg ccgtctcacg aacattacgc
    14161 actcgcccaa gcgcaaatgc ccaaaggcat cggcggcgtg gttacggttt atctcaaaaa
    14221 cgacacgcgc gaagcggcaa acagcgtgat taaaaacatg aaactggtca aaatgacctc
    14281 cagcctcggc ggcgtggaaa gtctggtcaa ccattgctat tcccaatctc acagcggcgt
    14341 gccgcataat gtgaaaatgg aaatgggcat cagggtcgga ctcctgcgct tctccatcgg
    14401 tattgaagac gtagatgata tttggaacga tatttccgcc gcactcgata caactttgta
    14461 aaccgtaaaa aatgccgtct gaaaccatag tttcagacgg catttcaata ttgcgaatca
    14521 aatatcaacg cttcagcatc tttgccgcct caatggcgta ataggtcaaa atcccgtccg
    14581 cacccgcacg tttgaatgcc agcaggcttt ccaaaaccac tttgccgccg tccagccagc
    14641 cgttggcaac cgccgcctgc aacatcgcat attcgcccga aacctgatag gcataagtcg
    14701 gtacgccgaa ctcgtccttc acgcggcgga caacgtccaa atacggcaaa ccgggcttca
    14761 ccatcaccat atccgcacct tcctgaatat cgagcgccac ttcatgcagc gcctcatcgg
    14821 tatttgcagg atccatctga taggtctttt tatctgcctt tcccaaattg cccgaactgc
    14881 ctaccgcatc acggaaaggg ccgtagaatg cagaagcata tttggcggaa tatgccataa
    14941 tccgcgtatg gatatgtccg gcatcctcca aagcctcgcg gatggcgccg atacgcccgt
    15001 ccatcatatc ggaaggagca acgacctgcg tgcccgcctc tgcatgacat aaagcctgtt
    15061 tcaccaagac ttctacggtt tcatcattca tcacgtaacc gttttcgtcc gtcagtccgt
    15121 cctgaccgtg caccgtataa ggatcgagcg cgacatccgt cataatgccc agttcgggaa
    15181 atctctcgcg caaggctcgg acagttgacg gcacgagtcc ttcgggattg tacgcctcct
    15241 gcgcacgccc ggttttgttt gccgtaacca cgggaaagag tgccaacatc ggaataccga
    15301 gcttcaccgc ctcttccgcc gtaaacagca gcctgtccaa actctgacgc ttcacgcccg
    15361 gcatagaagg cacatcctcc tcgcgcgccg ccccctccaa tacgaacacc ggataaatca
    15421 aatcatcggc ggtcagcata tgctcgcgca tcaggcggcg tgaaaaatca tccctgcgca
    15481 tacggcgcat acgcgaagcc ggaacattgc ggtaaggaaa ttgcataagc cctccaatca
    15541 tcctgtcaac aattcaaatc atacggaagc cgccgcaact tgcagacggc atcgagcggt
    15601 aatcaaaatc cgttttcacc ggaaagggtt tcggcacgcc gaaatgccgc gccgaaggac
    15661 agcgtgccgc ccattgaaag aacaatagca aaacaaccac ttcaggctgt caggcctcga
    15721 tattcttacg ccacaatacc aaaagtttcc cgatatgccg aacaggttgc gcgtcaaccg
    15781 cctcacacaa ggtattgcag atttcgatac gctcggcacg gtcgtcgccg aatacgcgca
    15841 ctttaatcag ctcatgcgcc gtcagtgccg catcggtttc cttgatgact gcgtccgtca
    15901 gaccctgctg accgaccatc acaacaggat ggagatggtg cgcgcgcgct ttcagttcca
    15961 aaatttcttt ggtgttcaat ttggtatccg tcattttcct actgtttgaa taaaaaggat
    16021 aatgcagcat tgtacgcgat ttggcacggc aacggcaaaa ttaacgtaca atacaccgtt
    16081 tacattccga cccgcgaacc actatggctg tacgttccaa atcctcaaaa gcgtggctgc
    16141 acgaacacat caacgaccac tacgtccata tggcgcaaaa agacggctac cgcgcccgtg
    16201 ccgcatacaa acttttggaa atcaacgaaa aagacaagat aatcaaaccc ggcacggtac
    16261 ttgccgactt gggcagcgcg ccgggaagct ggtcgcaggt tgccgccaag ctgacgggta
    16321 cttccggaac ggttttcgcc ttggacatcc tgcctatgga agccataggg ggcgtgtcct
    16381 tcattcaggg cgacttccgc gagaacgacg ttttggcgca attcgaaacc ttgttggaca
    16441 accgcccgct cgaccttgta atctgcgata tggcgcccaa tatgtcggga aacgccgtaa
    16501 gcgatcaggc acgcagcttt tatctgtgcg aactggcttt ggacttcgcc tcgcaacacc
    16561 tgaaaaccgg cggcagcttt ttggtcaaag tctttcaggg tgcaggctat caggaataca
    16621 tggcagccat gcgcgaaatt ttcggcactg tgcagacgcg caaacccgaa gcctcgcgca
    16681 atcgctccag tgagatttat ttattgggca aaaataaacg ctgacaatac agacggcgtg
    16741 ctttacaatc atttccgttt tacacctcaa ttatggagcc ttgctaagtg gggaacacct
    16801 ttaaatcaat ccttgtctgg gtcgccttgg gtatcggcct gatggctgcg ttcaacgctt
    16861 tggacggcaa aaaagaagac aacgggcaaa tcgaatattc tcagttcatc cgacaggtca
    16921 acaacggcga agtatccggc gtcaacatcg aaggatccgt cgtcagcggc tacctgatta
    16981 agggcgagcg caccgacaaa agcaccttct ttaccaacgc gcctttggac gacaacctga
    17041 ttaaaacact gctcgacaaa aacgtccgcg taaaagtaac gccggaagaa aaaccgagcg
    17101 cgctggctgc cctgttttac agcctgctgc ccgtcctgct gctgattggc gcgtggttct
    17161 actttatgcg tatgcagacg ggcggcggcg gaaaaggcgg cgcattctca ttcggcaaaa
    17221 gccgcgcacg cctgctggac aaagatgcca acaaagtgac ctttgccgat gtcgccggct
    17281 gcgacgaagc caaagaagaa gtgcaggaaa tcgtcgatta cctcaaagca ccgaaccgct
    17341 atcaaagctt gggcgggcgc gtgccgcgcg gcatcctgct ggcgggcagc ccgggtacgg
    17401 gtaagacgct tttggcaaaa gccattgcag gcgaagccgg cgtgccgttc ttcagcattt
    17461 ccggttccga ctttgtcgaa atgttcgtcg gtgttggtgc gagccgcgtc cgcgatatgt
    17521 tcgagcaggc gaagaaaaac gccccctgca tcatctttat tgatgaaatc gacgcagtcg
    17581 gccgccaacg cggcgcaggt ttgggcggcg gcaatgatga gcgcgagcaa acattaaacc
    17641 aattattggt tgaaatggac ggttttgaga gcaatcagac tgtaattgtg attgcggcaa
    17701 ccaaccgccc cgacgtactc gatcctgcgc tgcaacgccc cggccgcttc gaccgccaag
    17761 tcgtcgtccc cctgccggac atccgggggc gcgaacagat tttgaacgtc cattctaaaa
    17821 aagtgccttt ggacgaatct gtggatttat tgtccctcgc gcgcggcacg cccggttttt
    17881 ccggcgcgga tttggcgaac ctggtcaacg aagccgccct gtttgccggc cgccgcaaca
    17941 aagtcaaagt cgatcaaagc gattttgaag acgccaaaga caaaatctat atgggtccgg
    18001 aacgccgcag tatggtgatg cacgaagacg aaaaacgtgc gacggcgtat catgagtccg
    18061 gacacgcgat tgttgccgaa agcctgccct ttaccgaccc cgtccacaaa gtaaccatta
    18121 tgccgcgcgg acgtgcgctg ggtctgactt ggcagcttcc ggagcgcgac cgcatcagta
    18181 tgtataaaga tcagatgttg agccagctct ccatcctgtt cggcggacgg attgccgaag
    18241 acatcttcgt cggacgcatc tccaccggtg catcaaacga ctttgaacgc gcaacccaaa
    18301 tggcacgcga aatggtaacg cgctacggca tgagcgacaa aatgggcgtg atggtttatg
    18361 cggaaaacga aggcgaagtc ttcttggggc gcagcgtaac ccgttcgcaa aacatttccg
    18421 agaaaaccca gcaggacatc gacgcggaaa tccgccggat tttggacgag cagtatcagg
    18481 ttgcctacaa aatcctcgat gaaaaccgcg acaagatgga aacgatgtgc aaagccctga
    18541 tggaatggga aaccatcgac cgcgatcagg tactggaaat tatggcgggc aaacaaccca
    18601 gcccgcccaa ggattacagc cacaacctgc gcgagaatgc ggacgcggcg gaagataacg
    18661 cgccgcacgc tccgactcgg gaaaaaaccg aagcacctgc cccggcagac accgcctcga
    18721 cagagtccgg gcagcagcct gaaaacaagg cttaacttcc cgaacaaacg gcagcccgca
    18781 agctgccgtt ttcccatccc gttttcagac ggcattttcc gctcaatgcc gtctgaaacg
    18841 ccaaagccgt atcggttttt gcgaatctgc gatatggcgg tttaaattga aatcagaacg
    18901 gggcaaggct gtaccggttt aaaattaaac cgctataata ccgcccttcg aatacacgcc
    18961 cgcattggtg atgcccgtga aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga
    19021 caaaatgttc gagctggtcg acaaggttga ggattacccg cactttctgc cgtggtacag
    19081 caagaccgag gtcatcgggc gtagcggcaa cgaactgaag gcgcggctgt ttatggatta
    19141 tatgcgcgtt cgccaatcgt ttgccacgca caaccgcaac atccccggca gggaaatccg
    19201 tatggaactg ctcgaaggcc cgttcaaaac cttacgtgga acgtggaaat ttatcgattt
    19261 gggcgacgat atgtgcaaaa tcgaattcaa tttggaatac gatttttcca atgccgtttt
    19321 gtccgcctta atttcccccg tcttcaacca cctttccgcc acgctggtcg aagcgttcgt
    19381 caaagaggca gaccgccgtt atgcttgaaa ttgagattgt gtacggtctg cccgaccgac
    19441 aggttttgaa aaccatgcag cttgccgagg gaacaaccgt ccgcaccgcc gcactgcaaa
    19501 gcggtttgga cggcatattt gaaaatttaa acctgcattc cgcgcctttg ggcattttcg
    19561 gcaaagccgt caaagacgac acgccgctgc gcgacggcga ccgcatcgaa gtgtaccgcc
    19621 cgctgttgat cgaccccaaa gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac
    19681 catgccgtct gaagccttca gacggcattc gacagaaaca cggaaaatat catgtcaaac
    19741 acaatcaaaa tggttgtcgg cttgggcaac ccgggcaaag aatacgaaca gacccgccac
    19801 aatgcgggct tttggttcct cgacgaactg gcgtggaaat ggaaggcttc gtttaaagaa
    19861 gaaaaaaaat tcttcggcga agttgcccgc gccgccctgc ccgacggcga tgtttggctg
    19921 ctcaaaccgg ccacgttcat gaaccgttcc ggacaggcgg ttgccgcgct tgcacagttc
    19981 tacaaaatca aacccgaaga aatcctcgtc gtccacgacg aactcgacat cccttgcgga
    20041 cggatcaaat tcaaactcgg cggcggcaac ggcggacaca acggcttgaa agacattcag
    20101 gcaaaactcg gcacggcaga ctattaccgc ctgcgcctcg gcatcggcca ccccggcgac
    20161 cgcaacctcg tcgtcggcta cgtcttgaac aaaccgagcg cggaacaccg ccggcaaatc
    20221 gacgatgccg tcgccaaatc cctgcaggcc gtacccgaca tcatttccgg caaatgggaa
    20281 gaggcaacgc gcttcctgca cagcaaatga cccgatgccg tcttaagccc tttcagacgg
    20341 cattttcccg atttccgtat ccgaacagtc atgaccgaac tcaagcagct tatccaaacc
    20401 gaatccatcc ccgtcatcga agaaaccctc gatttcctgc tgtacgaatg cagcatcgac
    20461 gacgcaccgt ccgccgaaga agtggcacaa tggcgcgaca tacttgccgc acgcggcggc
    20521 aaattcctgc gcctgtccaa aatctgccaa acgtggctgg acgaggaggc ggcatgaagc
    20581 tgccgcgcaa ccgcttcagc ctgctttccg cattgtggtt tgccggcggc atctattcgc
    20641 tgctcttcaa agctgccgac accgcgccgc cgccgtttcc acatttcgac aaagcagcac
    20701 accttgccct gtttttcgca caaatcttgc ttctggccaa agcattcaaa accggaaaac
    20761 ttcccatccc ctaccgcagc ctgattgcgt tcgccttctg ttttgccgtc ggcagcgaat
    20821 gcgcgcaggc atggtttact gcaacgcgaa ccggcagttt gggcgatgtc cttgccgacc
    20881 tgacgggcgc agcccttgcc ctctttgccg cgcgttctgc ctgccgcccg gactaaatcg
    20941 gtttatttct cccaaacagg atgaatcccc ctgatgcatc ctgtttttcc ccttacatat
    21001 caatcccatc tcataaacaa aaccgacaaa tcgtttacaa tatatttaca ctacaccgaa
    21061 ttacaaatat actcgaagcg gttgcagaac cgacggtttc gcagaccgtt tcggattgca
    21121 cgacacaaaa gaaaaaccga ttttgttgcc ttaaaggagc attcatgaac ctgcatgcaa
    21181 aggacaaaac ccagcatccc gaaaacgtcg agctgctcag tgcgcagaag ccgattaccg
    21241 actttaaggg tttgctgacc accattattt ccgccgtcgt ctgtttcggc atttaccaca
    21301 tcctgcctta cagccccgat gccaataaag gtatcgcgct gctgattttc gttgccgcac
    21361 tttggtttac cgaagccgtc cacattaccg taaccgcact gatggtgccg attctcgccg
    21421 tcgtactcgg tttccccgac atggacatca aaaaggcgat ggctgatttt tccaacccga
    21481 ttatctacat ttttttcggc ggcttcgcgc ttgccaccgc cctgcatatg cagcggctgg
    21541 accgtaaaat cgccgtcagc ctgttgcgcc tgtcgcgcgg caatatgaaa gtggcggttt
    21601 tgatgttgtt cctcgttact gcctttctgt ccatgtggat cagcaacacc gccaccgccg
    21661 cgatgatgct gcctctagca atgggtatgc tgagccacct cgaccgggaa aaagaacaca
    21721 aaacctacgt cttcctcctg ctcggcatcg cctattgcgc cagcatcggc ggcttgggca
    21781 cgctcgtcgg ctcgccgccc aacctgattg ccgccaaagc cctaaatctg gacttcgtcg
    21841 gctggatgaa gctcggcctg ccgatgatgc tgttgattct gcccttgatg ctgctctccc
    21901 tgtacgtcat cctcaaacct aatttgaacg aacgcgtgga aatcaaagcc gaatccatcc
    21961 cttggacgct gcaccgcgtc atcgcgctgt tgattttcct tgccacagcc gccgcgtgga
    22021 tattcggctc caaaatcaaa accgccttcg gcatttccaa tcccgacacc gttatcgccc
    22081 tgagtgccgc cgtcgccgtc gtcgtcttcg gcgtggcgca atggaaggaa gtcgcccgca
    22141 ataccgactg gggcgtgttg atgctcttcg gcggcggcat cagcctgagc acgctgttga
    22201 aaacatccgg cgcgtccgaa gccttgggac agcaggttgc cgccaccttt tccggcgcgc
    22261 ccgcattttt ggtgatactc atcgtcgccg ccttcattat ttttctgacc gagttcacca
    22321 gcaacaccgc ctccgccgcc ctgcttgtac cgattttctc cggcatcgct atgcagatgg
    22381 ggctgcccga acaagtcttg gtattcgtca tcggcatcgc cgcatcttgc gccttcatgc
    22441 tgccggttgc cacgccgcct aacgcgattg tgttcggcac gggcttaatc aaacaacgag
    22501 aaatgatgaa tgtcggcata ctgctgaaca tcctatgcgt agtattggtt gctctgtggg
    22561 cttatgctgt actgatgtaa accatcgacc taaacaacaa gaccgtctga aagaatattt
    22621 ttcagacgac cttgaagttt gtcgttcaga cacaatttgt cgaataattc aaaaccggat
    22681 tctaacgaaa ggaaaaccca tgattatcct gcacaccaac aaaggcgaca tcaaaatcga
    22741 actcgatttc gacaaagccc ctgttaccgc caaaaacttc gagcaatacg tcaaagacgg
    22801 cttctacgac ggcgtaatct tccaccgcgt catcaaaggc ttcatgattc aaggcggcgg
    22861 catggatgaa aacatgaacg aaaaagaaac ccgcgatccg attcaaaacg aagcgtccaa
    22921 cggcctgccc aacgataaat acaccatcgc catggcgcgc acttccgacc cccattccgc
    22981 cggcgcgcaa ttcttcatca acactgccga caacgctttc ctaaacttcc gttctaaaga
    23041 gctgtacggc aaaaccgtcg tccaagactg gggctatgcc gtattcggta aagtcgttga
    23101 cggttttgac gttgtcgatg ccatcgaaag cgtatccacc aaacgccacg gctaccacga
    23161 cgatgtgccg accgaacctg tcatcatcat taaagccgaa gcagtataaa ccggcaatcc
    23221 gaaagcagcc tgcataaaag gctgctttta tttcagacgg catgttattt gatgtaaccg
    23281 ggctatgccg tctgaaaacc ggacgcaagc cttcagacgg cataccgcta tgttaaaata
    23341 cgcccgtttt tcctaaacgg acacaaagga aaccttatgg caagcatcac ccgcgacatc
    23401 ttcaaagcct acgacatccg tggcatcgtc ggcaaaaccc tgaccgacga tgccgcttat
    23461 ttcatcggca gggccatcgc cgccaaagcc gccgaaaaag gtatcgcccg catcgcgatc
    23521 ggacgcgacg gacgcttgag cggccccgaa ctgatggagc acatccaacg cggcctgacc
    23581 gacagcggta tcggcgtact caatgtcggc atggttacca ctcctatgct ctacttcgca
    23641 gccgtcaacg aatgcggcgg cagcggagtg atgattaccg gcagccacaa tccgcccgat
    23701 tacaacggtt tcaaaatgat gctcggcggc gacacgctcg caggcgaagc cattcaagaa
    23761 cttttagcta ttgttgagaa agacggtttt gttgccgccg acaaacaagg cagcgtaacc
    23821 gaaaaagaca tctccggcgc ataccacgac cacatcgtcg gacacgtcaa actcaaacgc
    23881 ccgataaaca tcgccatcga cgcgggcaac ggcgtgggcg gcgcgtttgc cggcaaactc
    23941 tacaaaggtt tgggcaacga agtgaccgaa cttttctgcg aagtggacgg caatttccct
    24001 aatcaccacc ctgatccttc caaaccggaa aacctgcaag atttgattgc cgcgctgaaa
    24061 aacggcgatg ccgaaatcgg cttggcgttt gacggcgatg ccgaccgctt gggcgtggtt
    24121 accaaagacg gcaacattat ttatcccgac cgccaactga tgctgttcgc ccaagacgtt
    24181 ttgaaccgca atcccggcgc gaaagtcatt ttcgatgtca aatccacacg cctgcttgcc
    24241 ccgtggatta aagaacacgg cggagaagcc ataatggaaa aaaccggcca cagcttcatc
    24301 aaatccgcta tgaaaaaaac cggtgcactg gttgccggcg aaatgagcgg acacgttttc
    24361 tttaaagaac gctggttcgg cttcgacgac ggcctgtatg ccggcgcacg cctcttggaa
    24421 atcctgtccg cctccgacaa tccgtccgaa gtgttggaca acctgccgca aagcatttcc
    24481 acgcccgaac tcaacatctc cctgcccgaa ggcagcaacg ggcatcaagt tatcgaagaa
    24541 ctcgccgcca aagccgaatt tgaaggcgca accgaaatca tcaccatcga cggcctgcgc
    24601 gttgaatttc ccgacggctt cggtctgatg cgtgcttcca ataccacgcc gattttggtg
    24661 ttgcgttttg aagcggatac gcaagcagcc atcgagcgca ttcaaaaccg attcaaagcc
    24721 gtcatcgaaa gcaatccgca tttaatctgg cctctgtaaa aatagaaaaa atgccgtctg
    24781 aaactttcag acggcatttt tcacaagtag ccaatcaaat cttggcagat tggatttggc
    24841 ttaaaaatct ccgcgtccgt tcgtgtttga ggtggtcgaa caactctttc gggctgccct
    24901 gctctacgat aacgccgccg tccatcacga cgacgttcgt ggcaacttcc agcgtgaact
    24961 tgatttcgtg ggtaacgacg accatcgtcc aaccttcccg cgccaattcc ttcatggcgt
    25021 ccaacacgtc ttgcaccaac tcggggtcca gcgcggaagt gggttcgtca aacagcatca
    25081 attcaggctg aatcgccagt gcacgggcga taccgacacg ctgctgctga ccgccggaaa
    25141 gctggtaggg atacaaatcc actttatcgc ccaagccgac tttttccagc agtttcaaag
    25201 cctcttcgcg cgcttgggcg gcaggcttgc cctgtacggc aaccggccct tccatcacgt
    25261 tttccaacac ggttttatgc gggaagaggt tgtattgttg gaataccatt ccggacttgc
    25321 ggcgcagtgc caaaatatcg tgtttgcttg tttttttgga aaaatcaatg cgtaacggcc
    25381 gcgcgttgtc gaactcgatt tgtccgtctt cgggcatttc caacgcgttt aggcagcgca
    25441 gaaatgttgt tttacccgag ccggaaggcc cgaggatgac gaccacctgc cctttgccca
    25501 catccaaatc gatgccgcgc aaaatggtgt tttcgccaaa ggtcttatgg atattgcgga
    25561 ttttaatcat gacaactcct tatttggcga cataacggtc gaaacgtttt tccaaacgcg
    25621 cctgaatcaa aaacagcact ttacagaaac accaataaac caatgcagct tcgatataga
    25681 caggcaaaaa gtcataagtg cggtttgccg tttcctgtgc gacacggaaa agctccgtta
    25741 ccgttaccac ggcggcaagc gaggtgtttt tgaacaagcc gataaactcg ttgctcaacg
    25801 gcggaacggc gacgcggaat gcctgcggtg cgacgatgcg gcggaacgtc tgcatatagg
    25861 tcataccgat ggagaaacct gcttcccact gccctttcgg cacggacaaa atcgccgcgc
    25921 gtatggtttc ggaagcgtat gcgccgacat tgagcgaaaa gccgatgatg gcggcgggaa
    25981 tcggattgat atagatgccg acggacggca gcccgtaaaa cacaatcaca agctgaacca
    26041 acagcggcgt accgcgaacg acggaaatat aaaattccac cagcttcaac aagcattttt
    26101 ggaaaatacc gccggaaggc atgattctta ccaaagcaac ggctacggca ataatcatgc
    26161 cgataacgaa agaagcgatc gccaaaggca aagacactgt aaagccggct ttaaccatgg
    26221 gccaaaacgc gctgatgagc atatcagcgc gtgtttccgt cataaacggc agagaggcaa
    26281 ggaaattatt gaacactgat gtcttttccg aagaattgtt cgcccagttt tttcaacgtg
    26341 ccgtcggctt tcagctcgtt gattgccgtg ctgaatttcg ccacggcctc gtcattgccc
    26401 ttgttgacaa tcagaccgga accgactttt tcatcggcag gcgcggacca cacgattttc
    26461 acccccgcat tcgggttttt cttcagatag tccaaaaccg ccaattcatc gttcaacgtc
    26521 gcatcggcgc gtttttgttc aatcagggtc agcgattgcg ccaaaccgtc caccggcacg
    26581 agttgcgcac ctgcggcttt ggctttttcg ccgtagttgc tggtcaggga ttgcgcggtt
    26641 ttcacgcctt tgatgtcggc tatggattta atgttgctgt cgttacgcgc aaccaaaacc
    26701 gcaccgctcc agctgtaagg ttcggatttg tcaaatgtcg cctggcgttc ggggctggtc
    26761 aggccgactt ggtttgccac cacgtcgaaa cgtccggctt tcaaacccgc catcatcgaa
    26821 tcccattgcg tttctttaaa ctcgactttt acgcccagtt tttccgccac ggcgcgggtt
    26881 acttccacgt cgtaaccggt cagtttgccg tctttgtcgt ggtaggtaaa cggtgcgtaa
    26941 gtgccttccg tgccgacggt aaccgtgcct ttattgttga tgcgctcgat taaagaaccg
    27001 gaaattgccg attgtgcagg cgcggaagat gctccgctgc cgccttccga accgccgcag
    27061 gccgccaaaa ccaatgcggc aatgccgccg agtacgaatt ttttcaacat cattccctcc
    27121 tgtaaaaagt gtgcaagtgc ggcattctaa gtgaaattgc ggcaaacaca aagaatactt
    27181 tttttatatg gttattccaa atcggaatgg gaaagatatg ccgaagccgc cggaagcgga
    27241 aataaagggg gcgggaaagg cggcgggcgg cacgaaaccg aaaagcggca tcaggtatgc
    27301 aaacggcgga ttcacgcacg ccgttccggc aaaaatccgc ctgccgattc ggtgctataa
    27361 tccacccgaa acagatgaca ccaaaaagga acatctcatg aacacgcccc aatccgccat
    27421 cattcccgac cacgcccaag ccggcatctt tatcgaagcc gacttcgccg ccaaccgcct
    27481 caacgatatt aaagccgcct gccgcgcttc gctcgacgcg ttatccgcct tgaaagcccg
    27541 ctttccggac gatattttgg gtctgaccat cgccttcggc agcaaagctt gggcaacatt
    27601 cggacacacg gacgaaggca gtgaaatcaa acccttcccc gaaatgggca acgggcttgc
    27661 gccgtccacg cagcacgata tgtccatcca catccaatcc ttccgccaaa acgccgccta
    27721 cgcgcttgcc caatccgttt tgggcgcgtt cggcgacagc atatgcgtcg cgtccgaaga
    27781 acacggtttg cgcctgtatc aggatcgcgg gctggacggt ttcgtcgacg gcacggaaaa
    27841 cccgcaaggc gatgaaacaa tccgcgaagt cgccatcatc cccgaagggc tgcccgatgc
    27901 aggcggcagc tatgtcctgc tgcaaaaata cctgcacgat ttgaaaaaat gggatgccgt
    27961 ccccgtcgcc gaacaggagg cctcggtcgg acgcagcaag gaaaccgacg acgaattcag
    28021 ccgcgatgtc cgcctgcccg attcgcacct cggccgcgtc aacctgaaag aaaacggagt
    28081 cggcctgaaa atcgtccgcc gcagcctgcc cttcggcaaa atcagcggcg aacacggttt
    28141 gatgtttacc gcatactgcc gcacgctgca taatatcgaa gcgcaattat tgagtatgtt
    28201 cggcgacacg gacggcaaaa ccgacctgct gctccgacac ctctccgccg ccgtttcggg
    28261 cggatactac tacgcgccgt ctgtcgaacg cctgcaaaac ctctaagccg gacgatgccg
    28321 tctgaaaccc tttttcagac ggcattttcc cgttttaccg tccaaccgtt ttcatacacg
    28381 ctatgtccgc acccatccga gcattcgacc cgctgaccgc ccccatttcc ggcaccaacc
    28441 tgattgaggc ctctgccggc accggcaaaa cctacggcat tgccgccctg tttacgcgcc
    28501 tgatcgtatt ggaacaaaaa agcgtcgagc gcgtattggt cgttaccttc accaaagccg
    28561 ccactgccga gctgaaaaca cgcctgcgcg cgcgtttgga cgatgtgttg caggttttgg
    28621 aaagcaaaga aattgccgaa cttggagacg acacgctttc agacggcatt gccgcctact
    28681 gcgccgaaca ccacgaaggc gacaccttcc tgcccgcact cttggaacag gctttgcaaa
    28741 aagaaagccg gacgcgtctg attgtccgcc tcaaggccgc catcgggcaa ttcgacaacg
    28801 ccgccatcta caccatccac ggcttctgcc agcgcatcct gcgcgactac gccttcctgt
    28861 gccaagcacc gttcgatgtc gaactgaccg aagaagacgg cgaccgcctg cttgtcccgg
    28921 ctcaagattt ttggcgggaa cgcgtcagcg gcgacccggt gcttgccgca ttggcgttta
    28981 aacgcaaagc tgtgccgcaa accgtccttg cccaaatccg cgcctacctg tcccgcccgt
    29041 acctgaattt ccgccgtccg caggcggatt tgaaacaggc gcagcgcgac gccgaaacct
    29101 catggcaaac cgtctgccgc ctgctgcccg agctggaagc cggcttttgg cgcatccatc
    29161 ccgacctcaa cggcaacagt taccgcaaaa acagcttcgg caaccttttt aaagaactgg
    29221 cacaaaaatc cgccgccgga caactgccct ttctggacaa agacacgcac gaacggctct
    29281 tgaaactctc atccgacaaa ctcgaagccg gactgaaaaa aggcaaaacg cccgatgcgg
    29341 cagtatttgc cgaattgcag aaactggcag acttcgggcg cgatttgaac gcactcgaag
    29401 aagcggaaga agcaacaatg atccggctgc aactggattt aatcgaatat ctcaacagca
    29461 gccttgccga aatgaaaaaa tcgcgccgcg aacgcggttt cgacgacctg ctgctcgatg
    29521 tccacaccgc gctgaccgac aatccgcacg ccgaaacccc cgcccgcgcc gttgccgaaa
    29581 actgggaaat cgcgctgatc gacgagttcc aagacaccga cccgctgcaa tacgaaatct
    29641 tccaaaagat tttcatcgcc cgaaaccgcc cgctgttcct cgtcggcgac cccaaacagg
    29701 cgatttacag ctttcgcggt gcggacattt acgcctacct tcaggcggcg gaagacgcgc
    29761 ggcaccgcta cacgctcgcc accaactacc gcagccacgc cgcgcttatc ggcagcatcg
    29821 gcgcgctgtt ccgcctcaaa gaacgcccgt tcgttttgga aaacatcggc tattcggaag
    29881 tcggtgcggc gcgtgccgaa agcaggttgt cgcccaaacg tcctgccgta cagctccgtt
    29941 ggctgcacga aaacgacaat gaaaaagcca acaaagacgt tttgcgccgc cgtgccgccg
    30001 actattgcgc cgacgaaatc gcccacgcgc tcaacgaagc ctccggaggc cgtctgaatt
    30061 tcaaaggccg cccgttgcag tcgggcgata ttgccgttct ggtacgcacg cacaacgagg
    30121 cggtgatgat ttccgccgca ctgaaaaaac ggcaggtgca aagcgtcctg ctttcgcgcg
    30181 aatccgtgtt cgcctcgccc gaagccgccg ccctgtccgc gctcatcggc ttctggctcg
    30241 aaccgcgccg cgccggaacg ctgcgctttg tcctgacaag cggcatattc ggttatgacg
    30301 cgcagcaatt gcacgacttc aaccaaaacg aaagcgagat tttgcattgg gcggaatctg
    30361 cccgaaccgc gctcgacata tggcaaaaat acggcatttt cgccgctatg cagcagtttt
    30421 cccaaacaca cggcatcgaa acgcgcctct taagccggaa caacgggcgc agcctgacca
    30481 attatttcca actgctcgaa ctgcttgccg ccgaagacgc gcaaaaccgc aaccccgccg
    30541 cactgcacaa atggctgcgc gatcaaatca gcctggccgg caacaacggc ggcgacaacc
    30601 gcgccatccg tttggaaagc gacgaagatt tggtcaaaat cgttaccatg cacgcctcga
    30661 aaggtttgca gtatccgctg gtgtactgcc cgtttgcgtg ggacgcgcaa gataccggac
    30721 cgtccgactg gcaaatcctc caccaaggcg caaaccgggc cgaacttttg gcaaaggcgc
    30781 aactgtcgga aagcgaacag aagcaatacg ccgacgaaga aatggcggaa cgcctgcgcc
    30841 tgctttatgt cgcgctgacg cgtgccgagg aacagctcaa catctacgcc gcctattctt
    30901 ccgataccgc cgacaacccc cttgcctacc tgatcgaagg cttgccgcaa gacagccgcg
    30961 aaaccgtccg ccgtacctat gcgtgtgaaa aagacggcat aacgatgctc aaacgcaact
    31021 ggcggcgcgt ggcggacaat gccccggccg gcacggattt cgccttcaca gaagatgcgc
    31081 cgccccctgc cgcctatcgc ggcaacgccg atcaagccgc cgaatttgcc gcaaacagca
    31141 ttcccgaacg cggattccga tttgtccgcc acaccagctt caccgcctta agccgccaaa
    31201 cccaaacgcc cgacggcggc gaagaagatg cctgcccgtc cttggatgcc gccgaaacct
    31261 cggtgccggc gatgccgtct gaaacgccga cggcttcaga cggcatatcg atacacgact
    31321 ttccgaaagg cacgcaggcg gggctgtgcc tgcacgaaat tcttgaagat ttcaaattcg
    31381 gacaagcagc cgccgaacag gaaaccctca ttgccgacaa gctgaaaaaa tacggttttg
    31441 aagaaatatg gctgcccgcc gttgccgaaa tggcggaagc ctgccgcaaa acgccgctga
    31501 ctggggcata cggcctgtcc gacatcccgc ccgggtgccg ccgtcccgaa atgggcttta
    31561 ccctccacac cgaagacttc ggcctcaaac ggctgcgcga ctggcttgcc cgcgacgaca
    31621 tcaggctgcc cgaagtctgc cgtgccgccg ccgagacgct cgacttccac accgtcaacg
    31681 gctttttaaa cggctttatc gatatggtct gccaagaccc cgacggcaat atctgcatca
    31741 tcgactacaa atccaaccac ctatccgcct acacgcggca ggcgatggac gaagccgtcg
    31801 cgcaccagca ctattacctt caggcactga tttacgccgt tgccgccgcg cgctacttca
    31861 aactgcgcgg acaaccgccc gccgccgttt ccgtccgcta cctgtttttg cgcggattgg
    31921 atggcaaagg cggcggcgtt tggcgttggg acatagatgc cgccgctttg gaacagataa
    31981 aataacttcc cgtcccttaa gacgacaacc gcaggagaac cctatgaata tcaaacaatt
    32041 gattaccgcc gcactcattg cctcagccgc ctttgccacg caggcagccc cgcaaaaacc
    32101 cgtatccgcc gcccaaaccg cgcaacattc agccgtttgg atcgatgtcc gttccgaaca
    32161 ggaatttagc gaaggtcatt tgcacaacgc ggtcaacatc cccgtcgacc aaatcgtccg
    32221 ccgcatatac gaagccgcgc ccgacaaaga cacgccggtc aacctctact gccgcagcgg
    32281 acggcgtgcc gaagccgccc ttcaagagct gaaaaaagca ggttatacaa atgttgccaa
    32341 tcacggcggt tatgaagacc tgctcaaaaa agggatgaaa tgacgcaatg ccgtctgaaa
    32401 ggacaggctt tcagacggca tacggcgttc cggcacgcgg cacggcatca ggcgcggcgc
    32461 gtgccgcccc gtccgaagcc cgataaacaa accgataagg aaatacgatg aaacacatac
    32521 tccccctgat cgccgcatcc gcactctgca tttcaaccgc ttcggcacac cctgccgaca
    32581 aaccgcccac ccaaaacgaa accgctatga ccacgcacac cctcacctcg aaatacagtt
    32641 ttgacgaaac cgtcagccgc cttgaaaccg ccataaaaag caaagggatg gacatttttg
    32701 ccgtcatcga ccatcaggaa gcggcacgcc gaaacggcct gaccatgcag ccggcaaaag
    32761 tcatcgtctt cggcacgccc aaggccggta cgccgctgat ggtcaaagac cccgccttcg
    32821 ccctgcaact gcccctgcgc gtcctcgtta ccgaaacgga cggcaaagta cgcaccgcct
    32881 ataccgatac gcgcgccctc atcgtcggca gccgcatcag tttcgacgaa gtggcaaaca
    32941 ctttggcaaa cgccgaaaaa ctgatacaaa aaaccgtagg cgaataagcc cgtcgccgac
    33001 atatggcgga tggaaatcaa atgcacacgc ccctcattcc cccgaagtcg ggaatccaag
    33061 cggttgggtt accgtgattt ccaatcattg ccgaaacgga cgattccgga ttcccgcctt
    33121 cgcagggatg acgggcgtat gcgttttgcc atatcacagg caactttgcc gcaaatctta
    33181 tctgtcgccg gtatgccttc ttgtttcaat ccgccacaaa ccaaatgccg tctgaaacct
    33241 gatttcagac ggcatttccg tttattccct gctgtcgcgg caaatatcga tggcttcctg
    33301 caaacttgaa acgccgtgga ttttcaggtt cggaaactct ttggcattgc gcggcatatt
    33361 ggctttgggg acgatggcgc gtttgaagcc gagcttttcc gcttctttga gccgctcttg
    33421 tccgcgtgcg acggggcgga cttcgccgct taggccgatt tcaccgaaga ccacggtttt
    33481 ttcaggcata gggcggttgc ggaagctgga aagcatcgcg aggatgaccg ccaaatccgc
    33541 cgccggttcg ccgattttca cgccgccgac ggcgtttaaa aaaacatcct gatcgaaaca
    33601 ggcgatgccg ccgtgccggt ttaacacggc aagcagcatc gcaaggcggt tttgttccaa
    33661 tccgacgctg aggcgtttgg gtgtgaagcc gtgcgcgtca tcgaccaatg cctgaatttc
    33721 gaccaaaagc gggcggctgc cttcctgtgt aaccaaaacg cacgaaccgg gcgtgtcgtc
    33781 gcggtagctg gcgaggaaga tggcggacgg gttggatacg cctttcaaac cgttttcggt
    33841 catcgcgaac acgcccaatt cgtttgccgc gccgaaacgg tttttgatgg cgcgtatcat
    33901 gcggtagttg gaatgttggt cgccctcgaa atacagcacg gtatcgacca tatgttccag
    33961 cacgcgcggg ccggcaatcg ctccgtcttt ggtaacgtgt ccgaccagta tcatggcgat
    34021 acccatttgt ttcgccatac gcgtcagttg ggcggcacat tcgcgcacct gcgacacgga
    34081 accgggggcg gacgtgattt ggtcggaata catggtttgg atggaatcga taacgacgac
    34141 ttcgggctga tgctgtttca aggccgcctg aatcgcttcc atgcggattt cggcaagcag
    34201 gtttacgcct tcggcgtgca gttccaaacg ttgcgagcgc agggcgacct gttgggcgga
    34261 ttcttcgccg gaaacgtaca gcactttgcg gctttgcgcc attttggcga tggtttgcaa
    34321 caacagcgtg gatttgccga tgccgggatc gccgccgagc aggatgaccg cgccatcgac
    34381 caaaccgccg cccaatacgc ggtcgagttc gcccataccg gtcggattgc gcggcacttc
    34441 ggcagcggta acggcggaga gggattggac ggtcgaagcg tccgccgccc aagattggaa
    34501 tcgggcgttt ttcggctcgg gcgcggcaaa gctttcctga agcgtgttcc actcgccgca
    34561 atgcggacat ttgccctgcc atttcgggga agtgccgccg cattcggtgc attggtaaag
    34621 ggttttaagc gtttttgcca tgatgttttc cctatgggat ttttgaagtg taaaaaaatc
    34681 gtctgaaact tcttttctaa aaaatttttt attattagtt tgttaatttt gattacaaga
    34741 ccatatcacg atacaaacaa gggtggcttt ctgttatttt gcggcataat gacggaaaat
    34801 acaaaacagg aatcccaaac aatgactttc ccattaaata taatggtaga
//
LOCUS       DAXDSD010000009        34417 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXDSD010000009 DAXDSD010000000
VERSION     DAXDSD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34417
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.009"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(184..1074)
                     /gene="sucD"
                     /locus_tag="ACFB2Q_000309"
     CDS             complement(184..1074)
                     /gene="sucD"
                     /locus_tag="ACFB2Q_000309"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC0917426.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(1085..2305)
                     /gene="sucC"
                     /locus_tag="ACFB2Q_000310"
     CDS             complement(1085..2305)
                     /gene="sucC"
                     /locus_tag="ACFB2Q_000310"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC0917427.1"
                     /translation="MGLAAGRFLKPTHLKEKSMNLHEYQAKELLASYGLPVQGGILAH
                     NGEEAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREKAKEVAESLIGTNLVTY
                     QTDANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETP
                     EKIFKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVN
                     PLAVRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEG
                     NIGCMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVL
                     INIFGGIVRCDMIAEAIVAAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADG
                     LNDAAEKIVAAVNA"
     gene            complement(2323..2610)
                     /locus_tag="ACFB2Q_000311"
     CDS             complement(2323..2610)
                     /locus_tag="ACFB2Q_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917428.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            complement(2730..4163)
                     /gene="lpdA"
                     /locus_tag="ACFB2Q_000312"
     CDS             complement(2730..4163)
                     /gene="lpdA"
                     /locus_tag="ACFB2Q_000312"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC0917429.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            complement(4424..5605)
                     /gene="odhB"
                     /locus_tag="ACFB2Q_000313"
     CDS             complement(4424..5605)
                     /gene="odhB"
                     /locus_tag="ACFB2Q_000313"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC0917430.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGSGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVTALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            complement(5705..8533)
                     /locus_tag="ACFB2Q_000314"
     CDS             complement(5705..8533)
                     /locus_tag="ACFB2Q_000314"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC0917431.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(8738..10021)
                     /gene="gltA"
                     /locus_tag="ACFB2Q_000315"
     CDS             complement(8738..10021)
                     /gene="gltA"
                     /locus_tag="ACFB2Q_000315"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC0917432.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(10133..10381)
                     /locus_tag="ACFB2Q_000316"
     CDS             complement(10133..10381)
                     /locus_tag="ACFB2Q_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC0917433.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            complement(10385..11092)
                     /locus_tag="ACFB2Q_000317"
     CDS             complement(10385..11092)
                     /locus_tag="ACFB2Q_000317"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC0917434.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(11212..12975)
                     /gene="sdhA"
                     /locus_tag="ACFB2Q_000318"
     CDS             complement(11212..12975)
                     /gene="sdhA"
                     /locus_tag="ACFB2Q_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC0917435.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(12978..13319)
                     /gene="sdhD"
                     /locus_tag="ACFB2Q_000319"
     CDS             complement(12978..13319)
                     /gene="sdhD"
                     /locus_tag="ACFB2Q_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC0917436.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(13313..13690)
                     /gene="sdhC"
                     /locus_tag="ACFB2Q_000320"
     CDS             complement(13313..13690)
                     /gene="sdhC"
                     /locus_tag="ACFB2Q_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC0917437.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(13977..15380)
                     /locus_tag="ACFB2Q_000321"
     CDS             complement(13977..15380)
                     /locus_tag="ACFB2Q_000321"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC0917438.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(15629..16366)
                     /locus_tag="ACFB2Q_000322"
     CDS             complement(15629..16366)
                     /locus_tag="ACFB2Q_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC0917439.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(16645..18921)
                     /gene="metE"
                     /locus_tag="ACFB2Q_000323"
     CDS             complement(16645..18921)
                     /gene="metE"
                     /locus_tag="ACFB2Q_000323"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC0917440.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(19057..19935)
                     /gene="metF"
                     /locus_tag="ACFB2Q_000324"
     CDS             complement(19057..19935)
                     /gene="metF"
                     /locus_tag="ACFB2Q_000324"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC0917441.1"
                     /translation="MNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGARP"
     gene            20132..20407
                     /locus_tag="ACFB2Q_000325"
     CDS             20132..20407
                     /locus_tag="ACFB2Q_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC0917442.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            20407..20532
                     /gene="ykgO"
                     /locus_tag="ACFB2Q_000326"
     CDS             20407..20532
                     /gene="ykgO"
                     /locus_tag="ACFB2Q_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC0917443.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(20618..20836)
                     /locus_tag="ACFB2Q_000327"
     CDS             complement(20618..20836)
                     /locus_tag="ACFB2Q_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917444.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKNAV"
     gene            20979..22118
                     /locus_tag="ACFB2Q_000328"
     CDS             20979..22118
                     /locus_tag="ACFB2Q_000328"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC0917445.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            22115..22696
                     /gene="metW"
                     /locus_tag="ACFB2Q_000329"
     CDS             22115..22696
                     /gene="metW"
                     /locus_tag="ACFB2Q_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC0917446.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            22813..23616
                     /locus_tag="ACFB2Q_000330"
                     /pseudo
     CDS             22813..23616
                     /locus_tag="ACFB2Q_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
     gene            23608..23715
                     /locus_tag="ACFB2Q_000331"
                     /pseudo
     CDS             23608..23715
                     /locus_tag="ACFB2Q_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(23791..24351)
                     /gene="efp"
                     /locus_tag="ACFB2Q_000332"
     CDS             complement(23791..24351)
                     /gene="efp"
                     /locus_tag="ACFB2Q_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC0917447.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVDYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(24394..25545)
                     /gene="earP"
                     /locus_tag="ACFB2Q_000333"
     CDS             complement(24394..25545)
                     /gene="earP"
                     /locus_tag="ACFB2Q_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC0917448.1"
                     /translation="MNTYAFPACWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHRLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(25564..25842)
                     /locus_tag="ACFB2Q_000334"
     CDS             complement(25564..25842)
                     /locus_tag="ACFB2Q_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917449.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            26193..27134
                     /gene="miaA"
                     /locus_tag="ACFB2Q_000335"
     CDS             26193..27134
                     /gene="miaA"
                     /locus_tag="ACFB2Q_000335"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC0917450.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGRPMSAHLNGQPEHTLPFELYTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            27138..27857
                     /gene="tadA"
                     /locus_tag="ACFB2Q_000336"
     CDS             27138..27857
                     /gene="tadA"
                     /locus_tag="ACFB2Q_000336"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC0917451.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIITSAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            27862..28350
                     /locus_tag="ACFB2Q_000337"
     CDS             27862..28350
                     /locus_tag="ACFB2Q_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC0917452.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILLILLVKYIAY
                     LF"
     gene            28416..29168
                     /gene="rlmB"
                     /locus_tag="ACFB2Q_000338"
     CDS             28416..29168
                     /gene="rlmB"
                     /locus_tag="ACFB2Q_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC0917453.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEGILENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDIGGNADLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(29230..30604)
                     /locus_tag="ACFB2Q_000339"
                     /pseudo
     CDS             complement(29230..30604)
                     /locus_tag="ACFB2Q_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
     gene            30984..31859
                     /gene="dapA"
                     /locus_tag="ACFB2Q_000340"
     CDS             30984..31859
                     /gene="dapA"
                     /locus_tag="ACFB2Q_000340"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC0917454.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHLSVIEETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            31868..33064
                     /gene="bamC"
                     /locus_tag="ACFB2Q_000341"
     CDS             31868..33064
                     /gene="bamC"
                     /locus_tag="ACFB2Q_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC0917455.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(33121..34053)
                     /gene="pip"
                     /locus_tag="ACFB2Q_000342"
     CDS             complement(33121..34053)
                     /gene="pip"
                     /locus_tag="ACFB2Q_000342"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC0917456.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPRLL"
BASE COUNT         8515 a         9306 c         8517 g         8079 t
ORIGIN      
        1 accttcaacg ttgagtgttg tactattttg tttttaatcc actatattct gactgcctca
       61 aagggcaaaa ataatgtgag tatgttgagg ctgcctcttt ttgaaatgac aaacaaaatg
      121 ccgtctgaaa cccgatttcc ggtttcagac ggcatttgat gttcaatcag ttgtcaaacc
      181 tgattatgcc aaaccttttg ctttcaacac ttccagcatc gtcgtgccca actcggcagg
      241 gctgcgggtg taggcgatac cggctttttc aaacgcggcg aatttttctt cggcagtacc
      301 tttaccgccg gagataatcg caccggcgtg gcccatgcgt ttgcctttag gagcggtaac
      361 accggcgatg taaccgacaa caggtttggt tacgttggat tggatgtatt cggctgcttc
      421 ttcttccgca gtaccaccga tttcaccaat catgatgatg gcatcggtat ctgggtcttc
      481 ttggaagagt ttcagtgcgt cgatttggtt cataccagga atcgggtcgc cgccgatacc
      541 gatacaggtt gattgaccca agcccagttt ggtggtttgt gccacggctt cgtaagtcaa
      601 tgtaccggaa cgggaaataa taccgatgcg gcccggagtg tggatgtggc ccggcataat
      661 gccgattttg cactcgcccg gagtaatcac gcccgggcag ttagggccga ccaaacgggt
      721 accgttgccg ttggtttcca agtaacgttt ggctttgagc atgtccagag ttggaacgcc
      781 ttcggtaatc acaacgacca agcctacgcc tgaatcaacg gcttcaacga tagaatccaa
      841 cacaaacgga gcgggaacgt aaatcacgga tgcgtcagcg ccggtttctt taacggcttc
      901 tttcatggtg ttgaatacag gcaggtctag gtgggtttga ccgcctttgc ctggggtaac
      961 gccgccgaca actttagtgc cgtaagccag agcttgttcg gagtggaaag taccgttttt
     1021 accggtgaaa ccttgaacca atactttggt gtctttatta atcaatacgc tcattctttt
     1081 ctccttaggc gtttacggct gcaacaattt tttcggctgc gtcattcaga ccgtctgcag
     1141 aagtcagttt cagacctgat tcgttcagga ttttcgcgcc gagttcggcg ttgttgcctt
     1201 ccaaacgaac aacgacagga acgttgacgt tgatttcttt aacggctgcc acgatggctt
     1261 ccgcaatcat gtcgcaacgt acgataccgc cgaagatgtt gatcaatacg cctttaacgg
     1321 atttgtcttc caaaatcagt ttgaacgctt caaccacgcg gtctttggtt gcgccgccgc
     1381 caacgtccaa gaagttggca ggttggccgc ctttgagttt gatgatgtcc atggtggcca
     1441 tcgccaaacc ggcaccgtta accatacagc cgatattacc ttccagggca acatagttca
     1501 ggtcgaattc agaagctttc agttcacgtt cgttttcttg agatttgtcg cgcaattcag
     1561 cgatttttgg caggcggtag agcgcgttgc tgtcgatgcc gattttgccg tccacgcaag
     1621 ccagtgcgcc gttttcgcga actgccagcg ggttgacttc aaacagggcg aagtcgtttt
     1681 cgacaaacgc tttgtacgcg ccggtcatca gtttggcgaa ctcgttgatt tgtttgtctt
     1741 tcaagcccag ttggaaggca acttcgcgag cttggcaagg ttgcaggccg accagcggat
     1801 caacggttac tttgaagatt ttttctggag tttcagcagc aactttttcg atttccacgc
     1861 cgccttcggt agaagccatg aatgtaacgc ggcgggtaga acggtcaacc actgcgccca
     1921 agtacagctc ggtttgaaca gggtacatgt cttcgcaaac caaaacgctg ttgacaggtt
     1981 ggccgttggc atcggtttgg taagttacca agttggtgcc aatcaggctt tcagccactt
     2041 ctttggcttt ttcgcggctt ttaacgactt ttacgccgcc cgctttaccg cggccgccgg
     2101 cgtgtacctg tgctttgaca acagcgaatt tgccgcccaa tttgtcgtaa gctgcagcgg
     2161 cttcttcgcc gttgtgcgcc aaaataccgc cttgtacggg caaaccgtag ctagccagca
     2221 gttctttagc ctgatactcg tgtaaattca tggatttctc cttaaggtga gtgggtttta
     2281 ggaatcgccc tgcggccaat cccatatttt cagacggtcc tactatttac tgaagggcaa
     2341 ccacatccgc gccagcagat acatgactgc accgcctccc ccgagaataa aaaagacgat
     2401 gcctttttta cgcgaattcg gacagagcag ataaacggca aggctgaaac tgataaacaa
     2461 caatgccacg cgccaaacca attctttatc tttgaaacgg ttgagcgtat aagtttcggt
     2521 catcatcaca tacatctgcc acaatgcagc catacacatg ctgatgatgc ccatcggtat
     2581 aaagaaaata ccgactattt ctttgttcat aatgaatgta aatgctgttt gtctgacgga
     2641 attataaatc agaaaacgaa gctctcggaa cattcaattt gccggaggcc ttaaggccgt
     2701 ctgaaaattt cagacggcct taatgtctat caaccgtgca aagcgcgttt gtcggccgcc
     2761 aatgcagctt cgtgaaccac ttcggacaag gttgggtggg catggataat acgggcgata
     2821 tcttcgctgc tggcgaagaa ttcgagcgca gtcacgcctt cggtaaccaa ttcgctgaca
     2881 accgggccga tcatgtgtac gcccaagata cggtcggttt tggcatctgc caacacttta
     2941 accgtacctt tggctttgcc cattgccaat gcacggccgt ttgcaccgaa acctgaagta
     3001 ccttttttgt actccacgcc ttcggctttg agctgctctt cggttttacc cacccaagcg
     3061 atttcagggt cggtgtaaat cacaaacggt acgttgttga agtcgatatg cggtttttga
     3121 ccggcaatgc gctcggcaac ggcaacgcct tcgtcgctgg ctttgtgtgc caacatcggg
     3181 ccgcgaacca cgtcgccgat tgcccatacg ttaggcaggt tggtacggca ttcgccatcc
     3241 actttgataa agccgcgctc gtctttttcc aagcctacgg cttcagcgtt caggcctttg
     3301 gtgtttggaa tacggccgat ggcaacgatc agtttgtcga atacttcggt tttggcttcg
     3361 ccggcagcag tttcgtaagc aacggaaaca cctttgcctt cagacttgat gtcgccgatt
     3421 ttcacaccca attcgatgct cagaccttgc tctttggtga agtatttgaa ggcttctttg
     3481 gcgatttgtt ggtcggcggc agccaggaag gtcggcgcgg cttcaagaat ggtaacttcc
     3541 gcacccacgc ggttccatac ggaacccatt tccaaaccaa tcacaccgga accgattacg
     3601 ccgagtttgg caggtacttc ggtcaggttc aatgcgcctt cgttgtccaa tacgttcacg
     3661 ttgtcgatag cgacttgcgg caacggacgc ggtacggaac cggttgctac gatgacgtgt
     3721 ttggcttcga taacggtttt ctcgcctttg ttatcgactt caatttggta agcatcgcca
     3781 tttttaccgg caaaggaagc agtaccgaac aggctggtta ctttgttttt ttggaacagg
     3841 aatttcacgc cgccggtcag tttggtcacg atggcatctt tgcgctcaat cattttggcc
     3901 acgtcaaatt ttacgtcgcc gacagtgata ccgtgttcgg caaaatcgtg ttgcgcagcg
     3961 tggaaatgtt cgctggattg caacagggct ttagaaggga tacagcctac gttcaagcaa
     4021 gtaccgccca atgcaggggc attgcctgct ttgttaacgc ctgcatcgac acaggcagtt
     4081 ttgaaaccca gttgtgcggc acggatggcg gcaacgtatc cgcccggacc tgcaccaatc
     4141 actactacat catattgaga catcgtgtca tcctttgctt ttagcagtat ctcaaaataa
     4201 aaacgatact tttcgtttct ttttttattt tgaatgactg cgttttgatg gttttaagaa
     4261 aaagccgtct gaaagtccat atctgccgat tcattcgaat caaatgtttc agacggcctc
     4321 aatcgttcgt tgagcctatt gtagcacgat actacattac gctacatcta aataaaaatt
     4381 cagacggcct tttcattaac aaataaaaag ccgtctgaaa cgattacaga tccaacaaca
     4441 ggcggaccgg gtcttccaac gcgtctttaa tggctaccaa ggtcaataca gcttcgcggc
     4501 cgtcaatgat acggtggtcg taagacagag ccagatacat cattggacgg acaacaactt
     4561 ggccgttttc aaccacagcg cgctctttag tggcatgcat acccaaaatc gcagattgag
     4621 gcgggttgat gatcggggtg gacatcatag aaccgaaagt accgccgttg gtaatactga
     4681 atgtaccgcc ggtcaggtct tcgatagcga ttttgccgtc tttggctttt ttcgcgtaat
     4741 caacaattgc ttgttcgatg tcggcaatgc tcatttggtc ggcatcgcgc aggattggta
     4801 caaccaaacc gcgtgggctg ccaattgcga taccgatgtc gaagtagccg tggtacacaa
     4861 tgtctttgcc gtcaacagaa gcattcacaa ccgggtattt tttcagggct gtaacagcgg
     4921 ctttaacgaa gaaggacata aagcccagtt ttacgccatg ttctttctcg aatttttctt
     4981 tgtacttcgc acgcaagtcc atgatcggtt tcatgttgac ttcgttgaat gtagtcagaa
     5041 tggcgttttc ttgttgagaa gccaagaggc gttctgcaac acgggcacgc aggcggctca
     5101 ttggtacgcg ttcttcagga cgtgcgccgg caggaagtgc aacggcaggg gccacggctg
     5161 cggcaggttt ggcagcggca ttttgtacgt cttctttcaa tacgcgaccg tcacggccgg
     5221 aaccttgcaa tacgttcacg tcaacaccgg tctcggcagc cagttttgca gcggcaggca
     5281 tggcggcgtt gttttgtgca gcagcaggaa cggcagccgg ggcagcttcg gcaggagcgg
     5341 ctgcaggtgc ttcagcagcg acagtagcag ctgtatcgat acgggccaaa acttggtcgg
     5401 caacaacggt ttcaccgtct tgcgctacga tttcaaccaa tacgccggct tgtggagaag
     5461 gtacttccaa aaccactttg tccgtttcga tatcgatcag gatttcgtca cgggcaactg
     5521 cttcgccaac ttttttcttc cattccaaga gtgtgccttc agatacgctt tcagacagca
     5581 taggtacttt tacatcaata atcattttgt gtctccaatg gccctttcag acggcctgtt
     5641 gtgtgttttt aattctgcat atgggcattc cgcctgagca gactcagacg gaatgctact
     5701 tggtttataa cgccaaagcg tcttcaacca attgtttcaa ttgagcaatg tgtttgcttg
     5761 agtagcccac tgcaggcgat gcgctgcttg gacggccggc ataagacagt ttttgctctt
     5821 cgctgataac gtcttcgatg cggtggcgga tttggtagaa cgcgccttgg tttttcggct
     5881 cttcttgcgc ccaaaccaca gattttgcgt tcggatattt cgccagttcg gctttaacct
     5941 cgtcgtatgg gaacggatac agctgctcaa cgcggacgat agcgacatca tcttccagtt
     6001 tacgttcggc tcgaccggct tccaagtcat agtaaacctg accggcacac aataccacgc
     6061 gtttcacgct gtcgttgctt gcgcgttcgg cggtatcacc aataaccgga cggaaagtcg
     6121 aaccttcggt gaagttttcc agcgggctca ttgcaccttt gaagcgcaac aggcgtttgg
     6181 acatgaaaat caccagcggt ttgcggtatg aacccaagac ttggcgttgc aagagatgga
     6241 acatttgcga cgcttcagac ggcatgatga cttgcatatt gttctcagaa cacagttgca
     6301 accaacgttc tacgcgtgca gaagagtgtt cagggccttg accgtcgtag ccgtgcggca
     6361 ggatggtggt cagaccgcac aagcgacccc acttggtttc gcctgaagac aggaattgat
     6421 caatagttac ttgcgcgccg ttggcgaagt caccgaattg agcttcccaa atggtcagct
     6481 tgtcaggagc ggagcaggca aagccgtact cgaacgccat cacggcttct tcgttcaaga
     6541 tagagtcgat aaccaggaac tcgcccaagc cttcgcccat gttgcgaaga ggaacataag
     6601 taccgtcgtc ccatttttcg cgtttttgat cgtgcagaac ggcatgacgg tgtgagaatg
     6661 taccgcggcc ggagtcctca ccggagatac gcacgccatg acctttggtc agcaggcttg
     6721 cgtatgcgag ggtttcggcc ataccccagt cgatggcttg tttgccggat gccatggctt
     6781 tacgcgcttc gatcacacgt tttgcagtcg gatgcagggc aaagccttcc ggcacagcgg
     6841 tgaatttctc agtaagacgc tcaatgtcgg cggcaggcaa accggtttcg attttttcgc
     6901 gccaatcttt accttggtat ttgctccagt cgatttgtgt gcgttggaag ttgctcaacg
     6961 ttgtttgctc aacatgttcg cctttgtcca aagcatcacg gtaggcttgg atgtaaccgt
     7021 cggcctcaac ttgagttacc acgccttcgg caatcagttg ctcggtgtac aaagcacgcg
     7081 cacccggatg ttgcgatact tttttgtaca tcatcggttg ggtcaaagtc ggatcatcgc
     7141 cctcgttgtg accccattta cggtagcaga caacgtcgat tacgatgtct ttatggaatt
     7201 ttttgcggta atccaaagcg gcttgaatgg caaagcaaac gcgttcggga tcatcgccgt
     7261 tcacatggat aaccggagcg gaaaccattt ttgcgatatc ggtacagtgt acggttgaac
     7321 gggtatcgcg gatatcggaa gtggtaaagc cgatttggtt gttgatgacg atatggaccg
     7381 taccgcctgt ggtataaccg cgcgttttag acaggttgaa tgttgcttgg ttgacaccca
     7441 aaccgataaa tgcggaatcg ccgtgaatca ataccggcaa gactttatcg cggccgtttt
     7501 cgcccaaacg tttttgtttg gcgcgtgcag aaccttccac taccggattg acgatttcca
     7561 agtgtgacgg gttgaacgcc aaagaaacgt gcatcgggcc gtgaggggtg gcaatatcgg
     7621 agctgaagcc catatggtat ttcacgtcgc cactaggcag tttgatttcg gcacggcctt
     7681 caaattcggc aaacaaatcg ccgggttttt tgcccaaaat gttcaccaaa acattcagac
     7741 ggccacggtg cgccataccg atgatgactt cttccacgcc gtccttaccg gcgttttgaa
     7801 tcaggtagtt caaaccggca atcgcgcttt cgccgccttc gacaccgaaa cgtttctgac
     7861 cgacatattt ggtatgcaga taacgttcca aagtctcggc agcggtcatc tctttcaaga
     7921 tacggcgttt ttgatcggca ttgtaatgcg gtgtggacaa tacgctttca aaatagttgc
     7981 gtacccaacg gcgctcttcg gtattgggaa tatagatata ttccaatgcg atgtggccgc
     8041 agtaggtttg tttgaggttg ctgatgattt gggacaaggg cagtttgccg cgattggcaa
     8101 aatcaccctc gcccatattg aatcgaagcg ccatatcggc atctgacaga ccgtggaatt
     8161 tcggatcgag ggcttcaata tcgcgcggag ggatacgttt gagcggatca agttgggccg
     8221 cacccacgcc ttggatacga taggcagaaa tcagccgtaa aacgctgact tgctttttca
     8281 gcattgcctc atccgcaccg cccgcaacgg cagatgcaat tttctttttc gccaaagtaa
     8341 caaatgattc gcgaatcggt gtgtgtgcga catcgacagc aaccgtcccc ggctgtttgc
     8401 tcaaatcggt gaaatactgc ttccattttt catcaaccgc atcggggttt tccaaaaaag
     8461 cctcgtacaa ttcctcaatg tagggtgcgt tcgaaccgaa cagataagag aaattgagtt
     8521 tttcgtccat catggcgtgc gctcttttct ttaaaatata aacaaagggt agaagtccgc
     8581 gtcctgacgg ctgaactgat gccgtctgaa aagacatttt attctaccct attttaaacc
     8641 ggattgcttg tcttgatttc atccgactac aatcagacgg attcaggaaa caatccggtt
     8701 ctctgatatt gcctgtttga caatatatta atgtatgtta cctctcgcct gccggcacat
     8761 aatcgcggcg ttccgaaccg gtataaagct ggcgcgggcg gccgattttc agcgaaggat
     8821 cgctaatcat ctcgtgccag tgcgaaatcc agcccacgct gcgcgacagg gcgaagatga
     8881 cggtaaacat ttcggtcggg atgcccagcg cggacaggac gatgccggaa tagaaatcga
     8941 cattgggata cagtttgcgt tcgataaaga acgggtcttt cagcgcaatc tgttccaatt
     9001 ccatcgccag tttgaatttc ggactgtctt ccaagcccaa ttccttcaaa acttcatagc
     9061 aggtttcgcg cataatgctg gcacgcggat ccatattgcg gtatacccgg tgtccgaagc
     9121 ccatcaggcg gtatttgcgc tgtttcacgc cttccatgta tgcggcaaca ttggacacat
     9181 cgccgatttc atccagcatt ttcaacacgg cttcgttcgc accgccgtgc gaagcacccc
     9241 acaggcaggc gataccggca gcaatacagg caaacgggtt cgcacccgaa gagcctgcca
     9301 gacggacggt tgaagttgag gcgttttgct cgtggtcggc atgcaaaata aagatgcggt
     9361 cgagcgcgcg tgccaaaacg ggattaggtt tgtagtcttc acacggtgtg gcaaacatca
     9421 tatgaaggaa gttttctgaa taagaaagat tattcttcgg ataattgaac ggcagaccgt
     9481 ttgaatagcg gtaacacatt gccgcaatgg tcgggatttt ggaaataagg cggtaaatcg
     9541 cgattttgcg gtgttcggga ttagtaatgt ccaagctgtc ttggtagaac gcagacagcg
     9601 cgccgaccac gccgaccatc atcgccatcg gatgcgcgtc gcggcggaaa ccccggaaga
     9661 accaagtcag ctgttcatgc accatcgtgt ggcggcggac tgtattgtca aattctgcct
     9721 tttgctcggg agtcggcagt tcgccgtaaa tcaacaggta gcagacttcc aaataatcgg
     9781 acttttcggc cagctgctcg atggggtatc cgcgataata aagcaagcct tgatcgccgt
     9841 cgatgtaagt aattttagac tcacagcttg cggttgaaac aaatccgggg tcgaaggcaa
     9901 acaaacctgt attttttgtc agcccccgaa tgtcaaccac atcatgcccg atgctggctt
     9961 ccaataccgg cagctccaaa cctgcccgac ccggtacgtt gagtttgatt gatttggaca
    10021 tattatcgct cctttgcaag ttataaccca cgttactgca tccgcagcac gttctttgct
    10081 ttcagacggc ctttgcattt tgaaatctgc cctcagacaa atttcagatg cctcatgccc
    10141 gtctgatttt ttcaagcatc gggataaggt gccctttgtc cgtttccgaa tgcccgtgaa
    10201 tcaaggcaag caattcttga tcttgaaatt caaggatttc ggaaaactcg gacagctctt
    10261 tatcgctcaa atgctcgaat tctttttcca taaacctgcc gaagattaaa tctagttcca
    10321 acaatccccg gcgggtttga aaacggattt tccgtttggc aatgtcgtca aaaaccatca
    10381 tttcttaaac aacccgtttc aacataatct ctttaatctt accgatggcc cgggtcggat
    10441 tcaagtgttt aggacatacg tccacgcagt tcataatggt gtggcagcgg aacaaacggt
    10501 acggatcgtt caaattatcc aaacgctcat tagtgatggt atctcggctg tccgcaatga
    10561 agcggtaggc gttcagcaag ccggacggac cgacgaattt gtcgggattc caccagaatg
    10621 acggacaggc ggtcgaacag caggcgcaca aaatacattc gtacaaacca tccaactctt
    10681 tgcgctcttc ctgagtttgc agacgctctt tgtccgcatc aatcggattg tcgttaacaa
    10741 cataaggttt gacggaatgg tattgtttga agaactgggt catatccaca atcaggtcgc
    10801 ggatgacggg cagaccgggc agcgggcgga ttttgacagg ctgtttcaag ctgcgtaaat
    10861 cggtcagaca cgccaagccg tttttgccgt tgatgttcat accgtccgat ccgcaaatcc
    10921 cttcgcggca ggagcggcgg aaagacaagg tatcgtcttg tgctttcagg cgtaccaaag
    10981 cgtccaaaag tttcacgtcg gtcggttcca attccaactc gtaacgctgc atataaggct
    11041 tggcatcaac gtccgggttg taacggtaaa tttcaaaact cattttttcc atgagaagtg
    11101 ttccttttct catcaactgc ccaatgattt tgtattataa attcaagcag ttcaatgtgg
    11161 gtatggttag gtttcagacg acctttgagg cgaagaccgt ctgaaaacgc atcaataaac
    11221 gcgtttggcc ggtttgatgt attccacgct caaaggcttg gtgtgtaccg gtttgtaaga
    11281 caaggtattg gtgtccgaat gatacagggt gtgtttcatc caattttcgt catcgcgctc
    11341 aggatggtcg tctgaagcgt gcgcaccgcg tgattcttta cgtgcttcgg cagacaccaa
    11401 agtcgctttc gccacttcaa tcaggttatc caattccaaa gcttcgatac gcgcggtatt
    11461 ccacactttg ctcttgtctt tgatttcggt acgtttcaca cgctcggcaa tcgccatgat
    11521 ttcttgaacg cctttgctca gaatctcatc ggtacggaac acgccggcgt gcagttgtac
    11581 ggagcgttgc agttcgcgac gcaatgcatc aacgttttca ccatcggttt gactgtccaa
    11641 acgctcgata cgttggcggg tcaactcacc ggcattagca ggcagaggtt tccaatcgct
    11701 ttgctctttg atgaatttaa tcatgctgtc accggcagct ttgccgaaca ccaccaagtc
    11761 cagcagggag ttcgtaccca aacggttcgc accgtgtacg gaagcacagg cgcactcacc
    11821 tgcggcatac aggcctttta caggtacttc gtactcgtcg ccttgcggaa caacaacttc
    11881 accgtgataa ttggtcggaa tgccgcccat catatagtgg gtagtcggca caaccggaat
    11941 cgggtcttta atcggatcga taccggcaaa ctgaatggaa atctcgcgga tgcccggcag
    12001 tttttccata attttttctg caccgatatg gtcgattttc agtaagacgt ggtctttgtt
    12061 tttaccacag ccgcgacctt catagatttc catcgccatc gcgcgtgaaa ccacgtcgcg
    12121 agaagccaag tcttttacgg tcggcgcata gcgttccata aagcgttcgc cgtcggcgtt
    12181 caacagaata ccgccctcgc cgcgtacgcc ttcggtaatc aacacgcccg cacccgccac
    12241 gccggtcggg tggaattgcc agaattccat atcttccaac ggaatgcccg cacgggcgca
    12301 aatgcccaaa ccgtcaccgg tattcatata agcattggta gaagaagcat aaatacgacc
    12361 gccgccaccg gtagcaaaca tcacggcctt ggcgtggaaa atataaactt cgcccgtttc
    12421 catttccatg gcggttacgc cgacgacatc gccgttttca tcacgaatca aatcttgcgc
    12481 cgtccattcc acaaagaatt gtgtattggc acggacgttt tgttggtaca aagtatgcaa
    12541 catcgcatga ccggtacggt cggcaaccgc acatgcacgt tctaccgcac gtttaccatg
    12601 ttcggcagta tgtccgccga aaggacgctg ataaattttg ccgctttcaa cgcggtcaaa
    12661 aggcataccc atgtgttcca actcaatcac cgcttcaggc gcagcgcgac acataaactc
    12721 aatcgcatct tggtcgccca gccagtcgga acctttcacg gtatcgtaca tgtgccagtc
    12781 ccaacggtcc tcctgcacat tacccagaga ggcggaaata ccgccctgcg ccgctacggt
    12841 atgcgagcgg gtcgggaaca ctttagacaa aacggcacaa ttcaaaccgg atttggataa
    12901 ttggagggct gcacgtaaac ctgcaccgcc accgccgaca atcacggcat caaacttgcg
    12961 aacaggaaaa cccatactta cccccaaatc actttaactg aatacacgag gcagccgacc
    13021 agccagacaa tggtggcaac ctgcaaaaac aaacgcacgc cgaagggttt gatatagtcc
    13081 atccacaaat cgcggatacc cacccaagcg tgcaagaata cggcgataaa gctcacttgg
    13141 gtaaatactt ttacccaagc ttgactaaaa aatgcctgcc atgccggata ttctttaggc
    13201 agggcaaata gaaccactaa aagtgcaacg gtataaatca acataataac cgcagtcgca
    13261 cgctgcatta cccaatcgcg caaaccgtaa tgggcaccgg tcaatttacg ttctaccata
    13321 acaacgctcc caaaacgaca gtcaaaacca atgcggaagc aaatacggct ttagcggtat
    13381 tgcgcgcagt attcagctca aggcctttgt gcgcatccaa aaataaaaag cggataccgg
    13441 cgagagaatg gtgcagataa gcccacaata taccgattaa aaccagcttg accaaaggat
    13501 gggaaacaat ggcacggtaa gtttcaaatg cagactcttg actcagggta ccggacagaa
    13561 aatacagcag gaaaggcagc ataataaaca gcccgacccc gctaatgcgg tgaaggatgg
    13621 aaactatccc gggtatcggc agacggatgt tcggcaaatc cagataaaca ggacgcagtt
    13681 tgacagacat agtcaattcc tctgtagtta tatatcgact caaaaaccgt aaacccattg
    13741 ctacactggg ttacatcaac gttaatttac ctgttttatc ccattttgaa cagtgtattt
    13801 taaaaacaaa ctgatagatt ttgacaaaca cgcgtggaaa cagcagatta aaagaaagat
    13861 ttttagtttt gccgctccct tcccgacacg gtatcagcat ccgaccccca accccatccc
    13921 caaaaataaa atgccgtctg aaaaaaattt caggcggcat tgttttgcca tatcggtcag
    13981 gctttcaatt tcgcatcggc atcgcgcaac gcggtacgca gaccttcctc gataacagga
    14041 tggtagaacg gcatatccag catttgcgga acggtcatct tcatttgatg cgcccatgcc
    14101 aacaagtgag ccaaatgttc ggcggcaggg cctacgattt ccgcgccgat aaagcggccg
    14161 gtggcttttt cggcatacag gcgcatatgg cctttgttta ccagcatcac gcggctgcga
    14221 ccttgatttt tgaacgatac ttcgccgatg acaaattcgt cggcttggta ttgcgcggca
    14281 acctgcgcgt atttcaaacc gacaaagccg atttgcggac tggtaaacac cacgccaatg
    14341 gtgctgcggc gcaaaccgct gccgatattc gggtagcggc ccgcgttatc gccggcaatc
    14401 ttaccttggt cggcggcttc atgcagcagg ggcagttggt tggacgcgtc gcccgcaata
    14461 aagatatgcg gaatgctggt ctgcatggtc agcggatcgg caacgggtac gccgcgcgcg
    14521 tctttgtcga tattgatgtt ttccaaaccg atattgtcaa cgttcggacg gcgacctacg
    14581 gctgccaaca tatattcggc aacaaatacg cctttttcgc catcctgctc ccaatggact
    14641 tctacattgc cgtctgcgtc gagtttgacc tcggttttag catccagatg cagtttcaat
    14701 tcttctccga acacggcttt cgcctcgtct gaaacaacgg ggtcggaaat gccgccgatg
    14761 attccgccca aaccgaaaat ttcaactttc acacccaaac ggtgcaatgc ctgacccagt
    14821 tccaaaccga taacgcccgg cccgaacacg gcaacgcgct taggcagcgt atcccacgag
    14881 aaaacgtcgt cgttgataat caaacggtcg cccaaagact gccactgcgg caggatgacg
    14941 ggacgcgaac cggtagcaat cacgaaactt tttgccgcaa tttggatatg gtcgtcgatt
    15001 tggacggtat gctcgtcgat gaatttagcc gagcccatga tgcgcttgtc ggcaggccat
    15061 tcttccacat cggtaacgac aaagccgaca aaacggtcgc gctcggattt gacgcgccgc
    15121 ataacctctt ctccgttgac gacgatgctg tctttgtcca aatgcacgcc gaaggggtcg
    15181 gtatgcaatg cgtggtgacg cgcttctgcg gcggcaatca agagtttgga aggcatacag
    15241 cccacgcgcg cgcaggttgt gccgaacaca ttgttttcaa tcaggtaaac attatccgaa
    15301 tgtaaacggg cattgcgaaa cgcacccata ccggcagtac cgccgccgat tacgacgata
    15361 tccgcttgaa tttttttcat aatctttgtc cttccgtttt cgtctgaaac attgccgaca
    15421 cagaacatat cggtttcaga cagcatttaa atatcttccc aatcaaaaac attattcaga
    15481 taaataaaaa caaaatttcg attgagtgcg acaatatcca aatcttcttg taaatataga
    15541 gttattcgga gcggcagtat agaaccgccc gaataactct atccggtata tcagacagtt
    15601 taatcaaact gccttaagca aacagctatc agtttttagc caagtaagct tccaaatctt
    15661 cgctgccgcc gatgtatttg ccgccgatga agacttgagg ggcagtcatc ttgccggtaa
    15721 tagcgcgaac ggaagtaacg gttgcatctt tgcccaatac gatttcttcg taagacaaac
    15781 ctttgtcttg caaagcttgt ttgactttgg cacagaattg gcaacctggt ttagtgaaaa
    15841 ttgccacaga ctcttgagcc ttccaatcgg gagcaacgaa tttcagcata gtatctgcat
    15901 cggatacttt gaaaggatcg cccggttctt caggttcgat gaacattttt tcaaccacgc
    15961 cgtcgttaac cagcatggag taacgccaag agcgtttgcc gaagcccaag tcttctttac
    16021 cgaccagcat acccatacct tcggtaaatt cgccgttgcc gtcaggaatc atgtagatgt
    16081 tgtctgattc ttcttcggca gcccaagcgt tcattacgaa agtatcgttt acagatacgc
    16141 agcagattgc gtcaacgccg ttttctttga acgcgccgaa taattcgttg taacgcggca
    16201 ggtgtgaaga agaacaagtc ggggtaaatg caccgggcag ggagaatacg actacttttt
    16261 tgcctttgaa caaatcatcg gtagacacat ctttccaagt gtcgccgacg cgggtgcgga
    16321 atactacgga aggtactttt tgaccggtac gatcttgcaa agccattttg agctccttaa
    16381 tagattggtt tgagaaataa ttgtgaatcg ccgcgcattc taccccatgc aaaatgattt
    16441 gtataattta tagtttaaat aatataaatt atttaaaact atcaaataca actccgattt
    16501 gcctgttccc cacatataaa gccgaattag agaaaaacaa gctgcaatga aaagattttc
    16561 cggcaactgc tcccctgcgc cgcaactcag gacaaaatgc cgtctgaaag gctttcagac
    16621 ggcatttgtt catacggtct cggcttattt cgccaattcg gcacgcagtt tgcgggttac
    16681 gttcatcatt acttgaagct gttccagagt ttctttccag ccgcgtgttt tcaggccgca
    16741 gtccgggtta acccacagac gctcaaccgg aacaacctca atggctttgc gcaacaggtg
    16801 ttccacttcg gcttctgtcg gtacgcgcgg gctgtggatg tcgtaaacgc ccgggccgat
    16861 gtcgttcggg tatttgaatt cgccgaacgc agccaagagt tccatgtcgg aacgtgaagt
    16921 ctcaatggtg atcacgtccg catccattgc agcaatcgca ggcaggatgt cgttgaactc
    16981 agagtagcac atatgagtgt ggatttgagt gctgtcttcg caacctgcag aggataggcg
    17041 gaaggattcg cccgcccagt tcaggtaggc atcccaatcg gcacgtttca aaggaagacc
    17101 ttcgcggatg gcaggttcgt caatttggat gactttaatg ccggcttttt ccagatccaa
    17161 tacttcgtcg ttcagagcca gtgcgatttg tttgcacacg gtagcgcgcg gaatatcgtt
    17221 gcggacgaaa gaccattgca gaatggttac aggaccggtc aacatacctt tcatcgggcg
    17281 tttggtcagg ttttgtgcgt aagtagacca agcgacagtc atagcttcag gacggcttac
    17341 gtcaccgaag atgataggtg gtttaacgca gcgtgagccg tagctttgca cccagccgta
    17401 ttgggtgaat gcaaaaccgc tcaacagttc gccgaagtat tcgaccatgt cgttacgctc
    17461 ggcttcgccg tgcaccagta cgtccaagtc cagtttttct tgctcttcaa ccaccaaggc
    17521 gatttctttt ttcattgcgg cttcgtaatc ggaggcagac agttcgcctt ttttgaaggc
    17581 tgcgcgtgct tggcggattt cggtagtttg cgggaaagaa ccaatgttgg tcgtcggcag
    17641 cagaggcagg ttcaaccatg cttgttgcgc tttgatacgg tcggcaaacg gtgatttgcg
    17701 ttggtctgcg ttgacaggca ggtcggccaa gcgtttggca acgtctgcac ggtggatttc
    17761 actgctgttg gcacgggagt cggcagcagc ttggctggcg gccagttctt cggcaacaga
    17821 atcacggcct tcattcaatg cggctttcag aacacgcaat tcttgggttt tttgcagagt
    17881 gaatgccaac caagagtaca ggtcgggttt gttggctttc agtttttctt cgactgacaa
    17941 gtcaaacgga gtgtgcagca gcgagcaaga gctggaaatc cacaaacgct cgcccagttt
    18001 ggcttgcaga ggcccgacgg tttccaaaac tttgttcagg ttggcgcgcc aaatgttgcg
    18061 gccgtcaatc acgccggcag acaggacttt gtcgtagccg gcgaacgcgt ccagttgctc
    18121 gggtgcgcgt accaagtcga tgtgcaggcc gtcaacaggc agggatttca acaatgcggc
    18181 gtgttcggca acagaaccga agtaagtgcc caacaggatt ttggcgctta ctttgttcaa
    18241 agtagcgtaa acgtctttgt atgcttctac ccattcttta ggcagatcga cagccaaagc
    18301 aggctcgtcg atttgaatcc actcggcacc ggcttcaacc aaagcagtca ggatttcaac
    18361 gtaaacaggc aacagtttag gcaacaggct cagacggtcg aattcaacgg agcctttttc
    18421 tttacccacc cacaggaaag tcaaaggacc gacaacggtc ggtttggctt tcaagcccaa
    18481 agtttgggct tcttgcagtt gttgaacgta gtgtttggcg ttggctttga attcggtatc
    18541 ggcatggaat tcaggcacca ggtagtggta gttggtgtcg aaccatttgg tcatttcgat
    18601 ggcgaattgg tctttgttgc cgcgcgccag ttggaagaat tgttccaaag gcaggttttg
    18661 gctatcgaag ccgaaacggg cgggaatcgc gccggtggca acttgcaggt cgaggatgtg
    18721 atcgtagaaa gtgaaatcac ctacagcaac gtaatcagcg ttggcagcag cttggtgttt
    18781 ccagtttttc tcgcgcaggt ctttggcaac gtcgagcagc tcttgttcac tgatctcttt
    18841 gcgccagtat ttttcttggg cgaatttcaa ttcacggaag gcgccgacac gcgggaagcc
    18901 tgagaaatgt aatgttgtca tgttgaactc tccagttgga ttttgtaagg accggttcaa
    18961 tccgaatcgg ccggtattgt aaatcagaat ggctcggata gggcggcgtt ttgcttttca
    19021 gacggcctcg gatgctgtct gaagccgttt tcggctttaa gggcgcgcgc ctaaaatatg
    19081 gcagatggcg taagtcagct cgctgcggtt gagcgtgtag aagtggaaat ctttcacgcc
    19141 ttcgcgggac aggactttaa ccatatcgat ggcgatactg gcggcgacga ggttgcgcgt
    19201 gccttggtcg tcgtccaagc cttcatacat ttgcgacagc catttcggga ttttgacgtt
    19261 ggttacttgt gccattttgc cgagttgctt gaagttggta acaggcagga tgccgggaac
    19321 gatttcgacg tcgatgccca acatcacgca gcggtcgcgg aagcgcaggt agcgttccac
    19381 atcgaagaag aattgggtga tgacgtggtt cgcgcccgca tcgattttgc gcttcagatt
    19441 aatcaggtcg gcttgtgcgg atttcgcttc gggatgtact tcggggtatg ctgctacaga
    19501 gatgtcgaag tcggcgacgg agcgtaatag cttaaccaag tcttcggcgt aaaacggttt
    19561 tttctcataa ccggccggct cgtcgccacg cagggcaacg atgcggcgga taccgctgtc
    19621 ccaatagtct ttggcgattt ggcgcaattc gtcgtgggat gcatcgatgc cggtcaggtg
    19681 cggagctgct tccaagccgg tttcctgttt gatgcgtttg acgatgccgt gcgtgcggtc
    19741 gcgttcgccg gagtttgcgc cgtaagttac ggatacgaac ttgggatgca gggtttgcag
    19801 acggtggatg gaatcccaca gcatcgtttc catttgttcg tttttcggcg ggaaaaattc
    19861 aaacgaaacg ttgatgttgc ctttcaaatc ggaaaggctg ttgtttaacg cattgatttc
    19921 ttttgcataa tttatctgca taccttcgtt atcttttaca tgatgtttca ggcggtatga
    19981 taaggttgca ccgaatatga gtcaatttca aaattttcag gtttatgatg aaaattttta
    20041 atatttagca aaaactatac gcttttcttg tacaagcctt ctaaattatg ataaagtttg
    20101 ttatgttata acacttcaat caaggcaata tatgaaaccg aatattcatc ctgacaatta
    20161 tcgaaccgtc ctttttttcg acagcagtgc caatgaaggc tggctgattc gttcttgcgc
    20221 cggaacgcac ggaaaaacaa tggtttggac agacggcaaa gaatatccgc tcttttcctt
    20281 agatacttcc tcttcctcac atcccgtcta taccggcaaa cagcgtaacg tcaataccga
    20341 aggccgtgcc agcaagttca accaacgttt ccaatccgtc atgtcatcgt ttagaaagga
    20401 caaataatgc aggttctgtc ttcgctcaaa accgccaaac aacgccaccg cgactgccaa
    20461 atcgtcagac gcaggggcaa agtgtacgtc atctgcaaaa gcaatccgcg ttttaaatcg
    20521 cgccaacgct gaccccctgc ccttcctgct tcggttgtaa agcccctcct accgcaagcc
    20581 cttttcaaat atgccgtctg aaacagaaaa atgcgtttca gacggcattt ttccgtcgat
    20641 acgaatccgg aatgtgtttg aaatacagca taaacagata ccaaaacagc gtcgcggcgg
    20701 ctgcaccgag cagtacccat gcgctgattt cggcgacttc gcgcaggaca cgtttctgcc
    20761 tgcggctaaa gaggcggttg ataatcagcg cggcaagtgc gaaaaagaaa aaaatacgga
    20821 aaagtctgcc tatcatgtcg ggaagcgaaa agtatggttc gggcgcggcg gcatgggaaa
    20881 ggatgtttca gcgcgtcagg ctgcttgtcc gaccgtgtca aaaagtcatg ccgtttgcga
    20941 tataataacg cttttcgccg catttgttgg aacaggatat gagtcaaaat acctcggtgg
    21001 gcattgtaac gccccaaaaa attccgtttg aaatgccgct ggttttggaa aacggtaaaa
    21061 ctttgccgcg tttcgatctg atgattgaaa cctacggcga gctgaatgct gaaaaaaaca
    21121 atgcggtttt aatctgccac gcgctgtcgg gcaaccatca cgttgcgggc aggcattcgg
    21181 cggaggataa atatacgggc tggtgggaca atatggtcgg tcccggaaaa ccgattgata
    21241 cggaacgttt tttcgtggtc gggttgaaca atctgggcgg ctgcgacggc agcagcgggc
    21301 ctttgtcgat caatcctgaa acgggcaggg aatacggcgc ggattttccg atggttacgg
    21361 tgaaggactg ggtaaaatca caagccgcgc ttgccgatta tctcggcatc gaacaatggg
    21421 cggcggttgt cggcggcagc ttgggcggca tgcaggcttt gcagtgggcg atttcctatc
    21481 ccgaacgtgt gcgccacgcc ttggtgattg cgtctgcgcc gaaactgtcc acgcaaaata
    21541 tcgcgtttaa tgatgtagca cgtcaggcga ttttgaccga ccccgatttc aatgaaggac
    21601 attaccgcag ccacaacacc gttcccgcgc gcggtttgcg gattgcccgt atgatgggac
    21661 acattacgta tcttgccgaa gacggtttgg gcaaaaaatt cggacgcgat ttgcgttcca
    21721 acggctatca atacggctat agcgttgaat ttgaagtaga atcctatctc cgctatcaag
    21781 gcgacaaatt cgtcgggcgg tttgatgcta atacatattt gctgatgacc aaagctttgg
    21841 actatttcga tccggcggcg gatttcggca acagcctgac ccgcgccgtg caggatgtgc
    21901 aggcaaaatt ctttgtcgcc agcttcagca ccgactggcg tttcgcgccc gaacgttcgc
    21961 acgaactggt caaggcactg attgccgccc aaaaatccgt gcagtatatc gaagtcaagt
    22021 ccgcacacgg gcacgatgcc tttttaatgg aagacgaagc ctatatgcgc gccgtaacgg
    22081 cttatatgaa caatgttgac aaggattgcc gattatgaac ctgcgcgatg atttgcaact
    22141 gatttacgac cggatacccg aaggcagccg cgtcttagac ttgggctgcg gcgacggcga
    22201 attgctggcg gctttggcgg aacacaaaaa atgcagcggc tacggcatcg aaatcgacac
    22261 cgacaacgtg attgctgcga tgtcgcgcgg cgtaaatgtt atccaagccg atttggaaga
    22321 agggttgacc gcattcaacg atcaaagttt tgatgtgatt gttttgagcc aaaccatcca
    22381 agcgatgcag aataccgaaa aaatcctgcg ctgcctgatg cgcctcgcca aacaggcaat
    22441 cgtcagtttc ccgaatttcg gctactggcg caaccgcgtc caaatcgcac tcggcggaca
    22501 tatgccggtt tccgaacgta tgccctacca ttggtacgac acgcccaata tccattggtg
    22561 cacgctcaaa gactttgatt tgttgtgcgc caaaaacaat atccgcgtcc tcgaacgcgc
    22621 cgtgatgacg ggcaacagac aggtcaaaca tttccccaac cttttgggca gccttgcgtt
    22681 ttaccgcgtc gggtaagaca ggttgaaaac cttgtcggca ataacgggtt tctcggttct
    22741 attaacaaca gtccgtctga aaaatgaaac ggcaggtttt cagacggcct tttcaatatt
    22801 ggaaacaata gaatgaaaaa caaaacgtca tcacttccct tatggcttgc cgcaatcatg
    22861 ctggccgcgc gttccccgag caaagaagat aaaacgaaag aaaacggcgc atccgccgct
    22921 tcgtcttccg cgtcccaatc cgatttgcaa ccggccgcat ccgcccctga taacgtcaag
    22981 caggcagaaa gcgcgccact gtgaaattgc accggcctgc accccgccgc cggcattggc
    23041 gatctcatac agcaaatcgc cgaacacatc gactcggact gtctgtttgc cctttcccat
    23101 aacgaactgg aaacccgttt cggcttaccc ggcggcggct atgacaacat acagcggctg
    23161 ctgtttcccg acatccgccc tgaagatccc gactaccatc agaaaatcat acaggcaatt
    23221 gaagacttgc gttacggaac gcgcaccatc agccggcagg cacaagatgc catgatggaa
    23281 caggaacgcc gcctccgaga agcgacgctg ctgctgacac aaggcagtca agaaacccgc
    23341 ggacaaggcg aggaaccgaa acgcgcacgt tattttgaag tttcggcaac atctgcctat
    23401 ttgaaccggc acaacaacgg actcggcggc aatttccaat acatcggcca attgcccggc
    23461 tatctgaaaa tgcacggaga aatgcttgaa aaccaatcac tcttccggct gtccaaccgt
    23521 gaacgcaatc ccgacaaacc gtttttagac atccattttg acgaaaatgg caaaatcacg
    23581 cgtattgtcg tttacgaaaa aaatatctat agtggattaa atttaaatca ggacaaggcg
    23641 acgaagccga agacagtaca gatagtacgg caaggcgagg caacgctgta ctggtttaaa
    23701 tttaatccac tatacttcaa tccaaacacg gggcgaatat aaaaaaatgc cgtctgaaag
    23761 cctttcagac ggcatttttc aagtaggata tcaggcgcgt ttgcggaatt cgccggtacg
    23821 ggtatcgatt tcgactttgt cgccgttttc gatgtaagac atcacttgga tttcggtgcc
    23881 gccgaccaga cgcgcggttt tcatcacttt gccggaagta tcgcctttga cggcaggctc
    23941 ggtgtagtcg acttcgcgca cgatgatggt gggcagttcg acagaaatgg gattgccttc
    24001 atagaaggta acttcgcatt ggtcttccat accgtcaacg atgaatttca acgcgtcgcc
    24061 gatgttgtcg gcttcgattt cgtattggtt gaattcttcg tccataaaga cgtacatcgg
    24121 gtcggcaaaa tagctgtacg tacagttttt gcgggacagg atgaccacgt cgaatttgtc
    24181 gtcggctttg taaatggttt cggaagcagc gccggtcagc aggtttttca gtttcatgct
    24241 gactttggcg gaagagcggc cgcctttgat gtattcggtt ttttgaacga ccataggatc
    24301 gttgccgacc ataaatacat tgccggcgcg cagttcttgt gctgttttca ttgtgatttt
    24361 ccaatcaaat cggttgtcgt aaaacgcgct attctagcgt attttttgat gctttgaaac
    24421 aaaggcggcg agtttttcgg atgcggaagg ctgcccgaaa agataacggc tccaatcctc
    24481 cgcgccttgc cgccagccgt tttgatgttg ttgcagggtt tgccaacatt cgaggcgttg
    24541 cgtggcggat aaagcctctc cgccgttgag gtcgtccgaa aggaggcggt gcaccgatgc
    24601 ggtttcgggc gtgtagaagc cgtatgcctt atcccaaaag gcgtggagtt tgtcgagatg
    24661 gacgttttca tcttgcgggt agatgtgcca aaagaaaggt tttccggcaa gctgggtacg
    24721 cacgaaactg tcttcgccgc gtatcacggc gcagtcggcg aggtgcagca atttgtcgaa
    24781 gtcctgttgc ggcacgaacg ggattttgac aaggcggacg gatgccgtct gaaagacacc
    24841 gccgtcattt tgcagggcgt tttgcggaat aacgccgctt tgtttgaggc tgtcgataat
    24901 ttgcgccccc gccagcagta gggtcatcag gctgcctgcc tgttgccaca tgtccagcca
    24961 ctttgcccaa acatcgcccc gatagccgaa aagcagccat tcgggggcgt ttttttcggg
    25021 cagcaccagc cgccggcgca gggcttcggt atcgaaacgg acggcttcgc ggtaatcgcg
    25081 ttcgcgtatc aacccgccgc ttttttcgct gaaacccata aaccaaaaat atttttgaac
    25141 gccctcctgc ggcgaaggca tcaggtgcag cctttcattg ctttcctccg cgctcaaata
    25201 ttcccaattc agccaaagcg gtttgtgtcg gcggatgatg ttcagcacat tttcgggcag
    25261 gtcgcaggca aaagtttcga taacggcatc gggaacgggc gcggtatcaa tgtctgccgc
    25321 atcggaatgc caagtgcgga catgaatatc ctgatgaacg aagggaacat cgggcaaatc
    25381 gggacaaagc gcgcgcaagg cggacacgtc gtccgtccac aaatgcacct gccaaccgag
    25441 ttcgcggtgc aaaacacggg cgagccgcca cgaaacgccg atgtcgccga aattgtcgat
    25501 gaccttgcaa aaaatccaac aggcaggaaa agcgtatgta ttcataacca tcgggaaaac
    25561 aagctaaaac gcgccgaaat agtggaacaa atacacaacc agcaaaacaa tcggaataaa
    25621 cagaaacatg gtttgacgtt tcaaatcaaa cccttgttta agatgatgct tgatggtttc
    25681 aaagaggacg aatttaaaca aaaacgtcgc ttcgcaggca aggatgatgc cgttgatcag
    25741 atgctggggt ttgtcttcgg gcggattggc ggcgaaaacg gcaagcgtcg ccaaggggat
    25801 gaggcagacg gcgagccagc ggacaagcag gtttcgggac attgcaaggc tttcaaaaaa
    25861 gcaaaacgcc attatcgcct caagaacgca ccttgtctaa cggacgcgcc ggagatgccg
    25921 tctgaagcct tgccgccgga aaacgccgca ccgccgaacc tttaaatata cccgggacgg
    25981 gcgttccccg agaaatcgga cgctgccgcc gatgcctgcg gacaaggcgg catcgggcgc
    26041 gggcatattg aaagcccgac gcatcggttt gaacgcattt tataaaggcg tacggcaaac
    26101 ccgccccttg ccgtcggcgt gcttccgcaa agcaaaatat aagggcggcg cgctttccgc
    26161 tataatccgc atttttccaa acctgaaaca ttatgccgac cccgaaagcc tttaccctgc
    26221 tcgggccgac cgcctgcggc aaaaccgcgc tcgccctcaa aattgccgaa acccttccgg
    26281 tcgaaatcat cagcctcgat tctgcactgc tctataccgg tatggacatc ggcacggcga
    26341 aaccgtccgc ctccgaacgc gcctttgtcc cgcaccacct tatcgacatc attacgcccg
    26401 ttcagacata cagtgccgcc cgttttgtcg aagactgcac gcgtctgacc ggcgaaatca
    26461 cggcgcgtgg gaaatgtccg ctgattgtcg gcggcacaat gatgtatttc cgcgccctga
    26521 cccaaggttt gaacgatttg cccgaagccg atgcctgcct gcgtgccgac ttggacgaac
    26581 aaaaacagat gtacggtttg gattttctct accgcaccct gcaaaaagtc gaccctgaaa
    26641 cagcatgccg tctgaaaccg aacgacagcc agcgcatcgg acgcgccctt gaagtctatt
    26701 acctgaccgg caggccgatg agcgcgcacc tgaacggaca gcccgaacac accctgccct
    26761 tcgaactcta caccgccgcc ctgattcccg aaaaccgcgc ccgactgcac gaaaacatcg
    26821 ccctgcgttt ccacctgatg cttgaacagg gctttatcgg cgaagtagaa aacctgcgcc
    26881 gccgctatcc cggcctgacc gccgactccc ccgccatccg ctgcgtcggc taccgtcagg
    26941 cgtgggaaca cctcgacggt gcaaccgaca gacaaacctt catcgaaaaa ggcattgccg
    27001 ccacgcgcca acttgccaaa cgccaactga cttggttacg caaaacacct ttagactgcg
    27061 ttgccgaccc gttttcagac ggcacttcag gcacgcgcct gattgaggcg gcaaaacggt
    27121 ttttcggcga ataaaccatg ctggccacgc cgccgctcgc ccccaaaacc gtcgccgccc
    27181 tgcacaggct cggtatccgt acgctcgaag aactgcggca aaacggttcg gtcaaagcct
    27241 ttctgctgct caaagcatcg ggtttgaccc tcacgaaaag cacgctgtgg caactcgagt
    27301 cgctgctgaa cggcacgccg ccgcaagaaa tgtcccaagc gcataaagac tgcctgcttg
    27361 ccgagttgaa aaaccatccg cccgtcgccg cctttccgcc gcaggaagaa atgggatact
    27421 tcatgcgcga agcactgcgc caagcagaac aatccgccgc cgacggcgaa atacccgtcg
    27481 gggcagtcat cgtttcagac ggcaaaatca tcacatcggc acacaacacc tgcattgccg
    27541 actgcaatgt cagccgacac gccgaaatca acgccttggc acaggcaggc agcgaaatgc
    27601 aaaactaccg ccttgacgga tgcgacatct acatcaccct cgaaccctgc gccatgtgcg
    27661 cgtccgcact gatacaggca cgaatcaggc gcgtgatcta cggtgcggca gaacccaaaa
    27721 ccggcgcggc aggcagcatc gtcaacctgt ttgccgacaa acgcctcaat acgcacaccg
    27781 ccatacgggg cggaatcctg caagaagaat gccgcgccgt attaagccgt tttttccaaa
    27841 acaaaagaaa aggttgaacc catgaccgcc ctactcgtca tcctcgccct cgccctgata
    27901 gccgtcggca cggcaggcat cgtctatccc gccctgcccg gcttggcatt gatgtttgcc
    27961 ggaacatggc tgcttgccta tgccggcggc tatcaaatct acggcgcagg catcttgtgg
    28021 acggtcggac tcatcagcct tggcggcata ctggcggact atatggcagg catgttgggg
    28081 gtaaaataca ctggggcagg caaactcgcc gtccgaggtg cattggccgg cagcatcatc
    28141 ggcatatttt tctccctgcc cgggctgata ctcggcccct ttatcggcgc ggcggcaggc
    28201 gaactgatcg atcggcgcaa tatgcttcag gcaggtaaag cgggcttggg tacgctgttg
    28261 gggcttgtcg tcggcacggc gttcaaaatc ggctgcgccg tatccatctt gcttatcctg
    28321 ttggtgaaat acatcgccta cctgttttaa ccccttcaga cggcataccg gcaaattgct
    28381 gtaatgccgt ctgaaatttc cgacaaagca ccattatggc aaaccaaaga cccatctacg
    28441 gcttccacgc cgtcaacgcc cgattgtggc aaaaccccaa atcgattacc gaactctata
    28501 tccaagaagg caaatccgac gcacgcacgc gcgaagtgct ggaaaaggcg gcaaacgaaa
    28561 acatccgcgt atattttgcc gatgccgacc gcctcaacgc catcagcaaa ggcgcgcgcc
    28621 atcagggcgt ggtcggattt atcgatgcct ccaaaaacca cgtccacctc gaaggcatat
    28681 tggaaaactt gagcgaaccg ccgctgctgc tgatactcga cggcatcacc gatccgcaca
    28741 acctcggcgc gtgcctgcgt accgccgacg caatgggcgt acacgccgtc atcgcgccga
    28801 aagacaaaag cgcgggactg aacgccaccg tcagcaaagt cgcctgcggc gcggcggaaa
    28861 ccgtccccta catcaccgta accaacctcg cccgcaccct gcgcgaattg aaagaatacg
    28921 gcatttggat tatcggcacc gacataggcg gcaacgccga cctttaccat tgcaatctgc
    28981 ccgacagcgc ggcatgggtg atgggcaacg aaggcgacgg tatgcgccgc ctcacgcgcg
    29041 aacattgcga catgctggtg tccataccca tgttcggcac ggtcgaaagc atgaacgtct
    29101 ccgtgtccgc aggaatggta ttaagcgaaa cccgccggca acgcgtattg aaaaacaaaa
    29161 aagcgtaagc ctcaaaaaca atgccgtctg aaacagggat tccgtttcag acggcattca
    29221 ttttcaaaat cagtgttcca aatggtcggt atcaaacttg accgccgcct cggttttatc
    29281 ttccggcaag accaaattca gcaagactgc cgtaatgccg ccgccggaaa tagagttttg
    29341 gaacaagacg ggcaggtttt taaacacttc cggctcaaac cccacgccca agcccaaacc
    29401 gaccaacgtt gccgcaatca ccgcctcgcg cctgcggatg ccgtggccga ccaaaatccg
    29461 cacgcccgca atcgcaatca ggccgaacat caaaaccatc gcgccgccca acaccggact
    29521 cggaatcgtc gtaaacgcgc gtccgacaac ggggaacaga cccaacagca ccaaaatcac
    29581 ggcaatatat ttgcccacgt ggcgcgaagc cactccggtc atctgaatca cgccgttgtt
    29641 ttgcgcaaac gtcgtcagcg gcagcgaacc caaagccgtc gcaatcaccg ataccaagcc
    29701 gtcagccaac acgccgccgc gcaggcgttt ggtgtattct tcgccttcaa tcggctggtc
    29761 ggacaccatt gccgttgccg tcaaatcgcc gaccgcctca aacacgccca acaagaaaat
    29821 cgcacccgcc gcaataaatg cgtgccagtc aaaagcaaaa ccatatttaa acggtacggg
    29881 cagcgtaacc ggcggcaggt tttgcagtgc ggaaaaatcc accttgccca aaaacagcgc
    29941 gacaatgtag ccggcaatca gaccgaccgc aatgccgctc atccgcagca gcggattttt
    30001 catgcagttg aacaccagca caatcagcag caccagcgaa gccagcccca agttttccat
    30061 cgagccgaac gtgccgtccg ccttcgcgcc gaagccgccg ccgaaatcgg caataccgac
    30121 gtgtaccaaa ctcagaccaa tcagcatcac gaccacgccg ctgaccgtcg gcgtaatcac
    30181 ttttttcaaa tacggcaaaa gccacgccga gaaacacacc aaaaacgcac cggcaaacga
    30241 tacgcccaag agcgtcgaaa ccatcgcccc ctcactcaaa ccgccctctt tcatccccgc
    30301 gccgagcgca atcatgacgg taacgctgga tggacagcat ccccgagccg accggcccga
    30361 agcggttgac ctgcaaataa gtgccgatgc cggaagccac catcgccatc gacacgagat
    30421 acgccgtcat ttccaccggt agttccagcg cgccgcccac aatcagcgcg ggcgtaatca
    30481 tcggcacgaa aatcgccaaa agatgggtaa ccgcgctcaa gagcgcatta ccgaacggcg
    30541 gcctgtcttc caaaccgtac accaaatcag gcgaatccgc ctgttttttc attgtttcag
    30601 ccattggagt ctttctatcc gttgccaaac gttaaaaaat tttaagaacg aaattgtaac
    30661 gaatcaaaga ttccttatca attcaaatcc tcatgtgcaa aaacaccacc ggttgtccga
    30721 attcaaattt ctttgaattt ccacaccgga cagtccttca tttccagaga gccttcatgc
    30781 gcttaacaca ttttaatcct caacggcgca tccactgctt aaacccaata atttcatgat
    30841 tataaaaaaa atattattta ttaagatatg atgggactta agccttggaa ccaaccgtgc
    30901 ggccgatatg tttcacacaa acaacaaagt aagctacaat ccgaagcata atcgacctca
    30961 ctcaatgaat aaaaggtttc cgtatgttac aaggtagctt ggttgccctg attaccccga
    31021 tgaatcaaga cggcagcatc cattacgacc aactccgcca attaatcgac tggcacatcg
    31081 aaaacggcac ggacggcatc gtcgctgtcg gcacgacagg cgaatccgcc accctctccg
    31141 tcgaagaaca tttaagcgta atcgaagaaa cggtcaaaca cgttgccaaa cgcgtccccg
    31201 tcatcgccgg cacaggggcg aacaacaccg tcgaagccat cgccctttcc caagccgccg
    31261 aaaaagccgg cgcggactac accctctccg tcgtccccta ttacaacaaa ccttcccaag
    31321 aaggcatgta ccgccatttc aaagccgttg ccgaagccgc cgcgattccg atgatactct
    31381 acaacgtgcc cggcagaacc gtcgtcagca tgaacaacga aaccatcctg cgccttgccg
    31441 aaatccccaa catcgtcggc gttaaagaag caagcggcaa cgtcggcagc aacatcgaac
    31501 tcatcaaccg cgcgcccgaa ggcttcgtcg tcctttccgg cgacgaccac accgccctgc
    31561 cgttcatgct ctgcggcgga cacggcgtta ttaccgtagc cgccaacgcc gccccgaaac
    31621 tctttgccga tatgtgccgt gccgcgctgc aaggcgacat tgcccttgca cgcgaactca
    31681 acgaccggct gattccgatt tacgacacca tgttctgcga acccagtccc gccgcaccga
    31741 aatgggcggt atccgccttg ggcagatgcg aaccgcacgt ccgcctgccg ctcgttccgc
    31801 tgacggaagg cggacaggca aaagtccgcg ccgccctgaa agcctcagga caactttaaa
    31861 tatagccatg ccgtccgaac cgttcggacg gcataacatg acaaacactt taatatccat
    31921 cacacaggat gacacgatga cccatatcaa acccgtcatt gccgcgctcg cactcatcgg
    31981 gcttgccgcc tgctccggca gcaaaaccga acagcccaag ctcgactacc aaagccggtc
    32041 gcaccgcctg atcaaactcg aagtcccgcc tgatttgaac aaccccgacc aaggcaacct
    32101 ctaccgcctg cctgccggtt cgggagccgt ccgcgccagc gatttggaaa aacgccgcac
    32161 acccgccgtc caacagccag ccgatgccga agtattgaaa agcgtcaaag gcgtccgcct
    32221 cgagcgcgac ggcagccaac gctggcttgt cgttgacggc aaatcccccg ccgaaatctg
    32281 gccgcttctg aaagcctttt ggcaggaaaa cggcttcgac atcgaatccg aagaacccgc
    32341 catcggacaa atggaaaccg agtgggcgga aaaccgtgcc aaaatccccc aagacagctt
    32401 gcgccgccta ttcgacacag tcggtttggg cggcatctac tccaccggcg agcgcgacaa
    32461 attcatcgtc cgtatcgaac agggcaaaaa cggcgtttcc gacatcttct tcgcccacaa
    32521 agcgatgaaa gaagtgtatg gcgacaaaaa caaagacacg accatgtggc agccttccgc
    32581 ttccgacccc aaccttgagg ccgctttcct gacgcgcttt atgcaatatt tgggcgttga
    32641 cggacggcag gcggaaaacg cattggcaaa aaaaccgacc cttcccgccg ccaacgaaat
    32701 ggcgcgtatc gaaggcaaaa gcctgattgt ctttggcgac tacggcagaa actggcggcg
    32761 caccggcctt gccctcgacc gcatcggact gaccgtcgtc ggtcaaaaca ccgaacgcca
    32821 cgccttcctg gttcaaaaag ccccgaacga aagcaatgca gttaccgaac aaaaaccggg
    32881 gctgttcaaa cgcctactgg gcaaaggcaa agcggagaaa cctgccgaac agccggaact
    32941 gattgtctat gccgagcctg tcgccgacgg ttcgcgcatc gtcctgctca acaaagacgg
    33001 cagcgcatat gccggcaaag acgcatccgc actgttaggc aaactccatt ccgaactgcg
    33061 ttaaaccgtt caaaacaaaa gcagcttcca aacagaagct gcttttttgt gcggaacttt
    33121 ttacaacaaa cggggcaaaa tatcctcaac cgcctgaacc aacgcatccg ccaaaggcgg
    33181 atcgaacgca caatgccccg cctggaccac cctcaattcc gcttcgggaa aggctttcga
    33241 cagctcccac gcactctgca taggcgtaca caaatcatac cgcccctgca cgataacggt
    33301 cgggatatgc cgtattttgc cgatattgtt caaaatcgcc ttatcaccct gcaaccaacc
    33361 gccgttgaca aaataatggt tttccaaacg cgcgattgcc agtgaggcat aagcatcttc
    33421 atccactccc tccggctcga aacgaatcag atagctttcc caatccgccc aagccttcgc
    33481 ggcagacagg cacacttctt catcttgatg aaacagcaac ccgtgatacg cctcaatcag
    33541 ccggttccgc cgattttcag caatcggcgc gacaaatttt tgccattgtt ccggataaat
    33601 ccggctcacg ccgcccgcct cgttcagcca cgccgtttca gacggcctgc acaaaaatat
    33661 cccgcgcaac accaatccct ttacccgttc gggatgggtt tgggcataag ccagcgacaa
    33721 agtgctgccc cacgaaccgc cgaacaccag ccatttcccg atacccagca tttcacggac
    33781 tttttcaata tccgccacca aatcccaagt cgtattgtct tccgcacaag catacgggtg
    33841 cgaacgtccg caaccgcgct ggtcgatgat gacgatgcgg aacacatcag gattgaaaaa
    33901 accccgacat tcaggcgaag cccccgcgcc cggcccgccg tgtaaaaaaa tgaccggcac
    33961 accgtcggga ttgcccgact cttcccaata aatttgatga atttcagaca cctgcaagta
    34021 tccgctatga aaaggctgtt ttatttcata catcatcttc tcccatattg cttctgacaa
    34081 tccgaacatc ccgacacgcg acatcccgca cgaaacacaa gccgccccga aaggcaaatg
    34141 ccgaaaaatc gcgataaaaa ccatcaatcg gaaaatattg cataaacagg cgaaaatata
    34201 tcattgaaaa gaaaacccat acaatgtctg acagaaacaa gccgctttgc cattgtttcc
    34261 taacagttaa accccgccct tcaaggcggc ggggcagggc ttgattcaaa atggcgcaag
    34321 cccctgccct caaatccaac acgcaggatt aaaccataat agcggctttc ttattatttc
    34381 ttattgaaac accgcccgga acccgatata atccgcc
//
LOCUS       DAXDSD010000010        34241 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXDSD010000010 DAXDSD010000000
VERSION     DAXDSD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34241
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.010"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(56..670)
                     /locus_tag="ACFB2Q_000343"
     CDS             complement(56..670)
                     /locus_tag="ACFB2Q_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917457.1"
                     /translation="MKKIMFGLLAAMAISNAQAEGNFLNLASKPAKIDGVLEASLDKP
                     YPKFVQDWMKDSKLAGLGRIIQGKIFESTGTIERKFYARSESEHEKTVEDAVGTFKAY
                     CEHHQGTVETLTSDIFCINGRQVQWMGWWYRENYTNFAFSDGYTGEQAYRSKVTDVQP
                     GKKISTSYGSALVLEKGENGLIKIQPNRGSERWITSDSIQVTYK"
     gene            complement(723..1793)
                     /locus_tag="ACFB2Q_000344"
     CDS             complement(723..1793)
                     /locus_tag="ACFB2Q_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917458.1"
                     /translation="MSRTNLKGGRKIALWRQTGYIGFYCCLILMMLFFPKDFNRLKII
                     AYTSPDGDEWVTALEAKLKHREGMIYYSRHRPGTRKKMVLTRRKATNFFRYYSEADSG
                     GASAPESLTHLLCKQVLNELSNLPGGLTTVLNCTEHAEQQPPVTIRLNRALSEYRIDI
                     DGKTFYIDVLLEFDQPGNTSLLRHEIRWQRKLAVEIWHTSRLASNAPKCLALSKIGIP
                     VVQIRADKGSFLYIDEDELLNYDNEEIKNRINRHVEKLRNTFRKQILCTLLRNPLSAD
                     FQTALMLHNQIKADEQQAEQIQEKFEALRNKHVLLEAEYSALAAQYAALLEHQNFQAH
                     SGKREIPKKHGILQRMASWFKS"
     gene            complement(1908..2357)
                     /locus_tag="ACFB2Q_000345"
     CDS             complement(1908..2357)
                     /locus_tag="ACFB2Q_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HFC0917459.1"
                     /translation="MPDSPMQINGYTIYEHECFTQQLDRLATTIESQKRSEKSSRSEE
                     AKLLERILGEIENIALNPDSPEYRLGKTLGPEYKFWRRAKFMGRYRLFFRYDSNSKII
                     ILAWVNDSGTKRTYGGKNDAYTVFKKMLGNGNPPSDWTALLEVVSKE"
     gene            complement(2359..2670)
                     /locus_tag="ACFB2Q_000346"
     CDS             complement(2359..2670)
                     /locus_tag="ACFB2Q_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFC0917460.1"
                     /translation="MKIIFDAVSTMTSKNQTTIPESVRKALGLEKQDKIRFSVLGGGQ
                     VLLEKNNTEEEEFEHDPVVGKFLHFLENSMTKNPNSIKPTSQSRFNRLRQLTWETDMK
                     D"
     gene            complement(3074..3538)
                     /locus_tag="ACFB2Q_000347"
     CDS             complement(3074..3538)
                     /locus_tag="ACFB2Q_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917461.1"
                     /translation="MIENQIDKEITQASCEGRFILKQENGKRFLYLNLPEGSDELNTI
                     WQTDEYDFTVPDLEVSIDVESLHTAVRLLNENQGILHGISTKCSAYSFGFEGKLRYER
                     LDVKPFPIKSFSYYLEFYNDWTGTLYELDLSAFLDEFFGECDPESKLDACLK"
     gene            complement(3627..4406)
                     /locus_tag="ACFB2Q_000348"
     CDS             complement(3627..4406)
                     /locus_tag="ACFB2Q_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917462.1"
                     /translation="MPNWCSNSLHFQGNPQRIAEVAEQILIKTDKGYQFSFQHIVPMP
                     EELILPEVPDSYMQQTWFQIAQQPEKKLINLEREMLEQRDKSFWSVQYRLPDELFSEL
                     KLYFSEKFDWDNLTVGEVFLHFEANSEVASRFRYDEPFAKRIAENIRQYGHPNWYDWR
                     LANWGCKWDVNPDCTFVTVGESGIRISCDTAWGPPEGIYRELAKRFPDVEFEAKYLEE
                     GMWFAGTYEGHEGALFDYPCTDDGVRDFATEHFGCEYDDED"
     gene            complement(4977..5492)
                     /locus_tag="ACFB2Q_000349"
     CDS             complement(4977..5492)
                     /locus_tag="ACFB2Q_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917463.1"
                     /translation="MSIKVDNILLIEGTKESVAEAAKLILFRSPVHPEHWAFDFNRIL
                     PLPENITSDLAKQRIEAWGSSFSPYPTETFVTQREGFIEICFLTGLAPPFGIYRKLAE
                     IFANLDVCFTAKYINQYGSFGGRYEYRNSVLYHCLCEMADIRKFGIAEFDIWYEGLSD
                     RLESDGYLVED"
     gene            complement(5584..6165)
                     /locus_tag="ACFB2Q_000350"
     CDS             complement(5584..6165)
                     /locus_tag="ACFB2Q_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917464.1"
                     /translation="MQNFIVWMNPDREESAAAAAKLNGAVLTAHTDGVMAAIRRELKR
                     FGLGADLAVFAQTCEEFAKQANADMVCISSSNGDAVAAVTYLEDGYAGKGVSAVADMR
                     YLMPAYRQYFNRPDKMVIFQGALQYLSDEDIAWIFRRHINAIIEWQSNEGLGLYEFGF
                     SLSDVLTDGCRHVLREVVTGWLNREILPELYNV"
     gene            complement(6401..6742)
                     /locus_tag="ACFB2Q_000351"
     CDS             complement(6401..6742)
                     /locus_tag="ACFB2Q_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917465.1"
                     /translation="MEQLDSAAPAACDTPSLFDLGRVSMTATVDYRAKEHLPAQLYEN
                     SLYAQILLEAHRHGIWGDIPPEDAKPNQLALKPGEEGRIMSSYKIGDQKIWVVTEWDR
                     SLTTLLFPEDY"
     gene            complement(6819..7940)
                     /locus_tag="ACFB2Q_000352"
     CDS             complement(6819..7940)
                     /locus_tag="ACFB2Q_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HFC0917466.1"
                     /translation="METQNHLMHPRVAHNHRDNGYFPTDQATLSRIAACLDIPASAGG
                     LLRIFDPCCGTGEALASLASTLQSEKISCQTYGIELEKDRAQEAMSRLTSVIKSDVEN
                     CTMQGSAVGLLFLNPPYGFAAKDNLSNQRTKRLEELFFARTFGTLQPGGVLVMIVPEA
                     ALTEHLTGEIATHCTDVRMIRAAADTYKQLVIFGIRPKNKASISKKQADAQQRLLLDF
                     ENAPETLPAGMPAYCVPIAPNKVFRPRSFKLEQDILERELGQQQSRTLWPSFSQYFGT
                     SAVIAEKRRPLCALGQWHAALALAAGQVNGVVTSTSGRKLLVKGSTHKTKVETKSEEY
                     GKKDRLVVTITSTDRFSPSIRAIDLTEGSSDFGRVLTIK"
     gene            complement(8011..8235)
                     /locus_tag="ACFB2Q_000353"
     CDS             complement(8011..8235)
                     /locus_tag="ACFB2Q_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917467.1"
                     /translation="MRYSKNKDYQFFIRQLVSGGEWMFLPKNGRKHSALKHLPTDRKI
                     PIPGSPGQDPRGLLNFKTMVRHIERGGTFD"
     gene            complement(8788..9045)
                     /locus_tag="ACFB2Q_000354"
     CDS             complement(8788..9045)
                     /locus_tag="ACFB2Q_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917468.1"
                     /translation="MKFFIFIKPDLPFHKESIESWIKLLEDIGKTALLAMLPLFWLNS
                     YSLTERASGVAVLALVAYFVQVYADKLRKYRDSLNRKDTSC"
     gene            complement(9293..11776)
                     /locus_tag="ACFB2Q_000355"
     CDS             complement(9293..11776)
                     /locus_tag="ACFB2Q_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFC0917469.1"
                     /translation="MYNPVSKIDELLPDFAGLDQTVTEMQERQNQITTDFNFIIRQVA
                     ETAQKSTEAAVLPEESPETELPDSDIPLTQFLAEFGDSLFNAVTEQNQPVYQGQTYPL
                     LEEVLDNLERPLFAAQREVVRAVLQQLVAEDKPAAIINAEMGTGKTMMSVAAAAAAHQ
                     AGLHRTLVLSPPHLVYKWRREILKTVPNARVWILNGADTLAKLLQIRALGRKPEVPEF
                     FVMGRVRMRMGYHWRPSYAVRRRRIPSEGASEGFAVSEYYCPKCGCEILDEENKPYNT
                     EGGVKNALAKNRRFCKGNHHGKPCKEPLWTLCRKEQSEDGVVSTVYDRVLKTMTTLPG
                     IGPKTAAKIIGMYGEQYLAEILENNIQAFSNLMDENGEFLFDDRQAARIDRDLGKAEF
                     SLGRGGYQPTEFVKRYLPKNFFGLLVVDEGHEYKNYGTAQGQAMGVLARCCNKILCLT
                     GTLMGGYAEDLFFLLWRLWPQMMIEDGFSYNKGNTLGSASMAFMRKHGVLKDIVRHLG
                     TEYSNGAFSSSKAERNSVRTAKAPGFSPLGIMRYVLPITVFLKLRDLGEGVLPGYKEV
                     FRPVEMTEDQQAVYKNMESVLRQHLKVALRNRDNTLTGVVMSALLSWPDCCYKPQDVY
                     WRRQNKILFHTEPQFDEEKISPKEADMLEVVKDSLAKGRKCLVYTVYTDTRDTTGRLK
                     EIFKRHGIKAAVMKATVKADQREDWVEDQLASGVQVVICNPELVKTGLDLLAFPTIYF
                     LQTGYNVYTLMQAARRSWRIGQKEDVEVYFAGYSDTAQQICLELMGQKVAVTQSTSGD
                     MPDSGLDILNQAEDSVEVQLAKRFVDRQD"
     gene            complement(11872..12363)
                     /locus_tag="ACFB2Q_000356"
     CDS             complement(11872..12363)
                     /locus_tag="ACFB2Q_000356"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC0917470.1"
                     /translation="MPLPDFRIRLLDSADDRNAFERPSEPLNRYLQKQVSQDVKRKVA
                     TCFVAVTSDGQILGYYTLAATGVALSNIPAGFRKKLPRYPTVPAVLMGRLAVDGKYGG
                     FGLGSLLLADALKRIGKAEIAAYALIVEAKDEAAIGFYRHFGFTSFEDATKHLFFPLA
                     NLR"
     gene            complement(12365..12649)
                     /locus_tag="ACFB2Q_000357"
     CDS             complement(12365..12649)
                     /locus_tag="ACFB2Q_000357"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFC0917471.1"
                     /translation="MFMTATYADCRFEARITSDTRALLKHAAELQGRSMSEFVISAAR
                     EAAQKAVADAEILMLSERDQLQFAQALLNPSKPNGALSRALEKHDELFGR"
     gene            complement(12882..13289)
                     /locus_tag="ACFB2Q_000358"
     CDS             complement(12882..13289)
                     /locus_tag="ACFB2Q_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917472.1"
                     /translation="MPTLSEKHEVLSGLNRQDAAAIVALIDLKTEDDMEKVLNKLDFM
                     TQNFDARLDAFEKSVDARISSFEKSVDARISSFEKSVDARFSSFENSVDARISSFENS
                     VDARFSAIDTKINAIYWFIGITTTVAVAAAKFL"
     gene            complement(13515..14096)
                     /locus_tag="ACFB2Q_000359"
     CDS             complement(13515..14096)
                     /locus_tag="ACFB2Q_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917473.1"
                     /translation="MLYLTSRNDLMADAFFIWNRQLMFASLHGRDADMLSFQAQLQVS
                     NERLGFRRPEEVLSCPRLTTAEHCLNLSKYMTKYQTLNYGSVTHMFLYSDILIQPNFD
                     SKTGWVMLDDVTADLDKAAWQLVRQLSDIPLLEHWQNAVLSVLEADKSIMRFTPRIDE
                     EYAVVGVQAVRVDIPEDFDVRLTAMLQSGRLHT"
     gene            complement(14106..14621)
                     /locus_tag="ACFB2Q_000360"
     CDS             complement(14106..14621)
                     /locus_tag="ACFB2Q_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HFC0917474.1"
                     /translation="MSINLSGTLTVQVIQGRRGPFKTAKLATSIGTFDVKKPILKQFE
                     PGEYQGNFIIEQLKVKAYEWGGGATSYLDAALDWQHLAEFAQEFSSGSDSPQDDPSDP
                     FDPVPENGIRLPEYAETADQVGEMIAQNREHISLGEYILSDRDELNIARSMMKEAGYS
                     YRPQSQTWCLQ"
     gene            15462..15785
                     /locus_tag="ACFB2Q_000361"
     CDS             15462..15785
                     /locus_tag="ACFB2Q_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917475.1"
                     /translation="MTYGREGHSGKLPKGQPSNSCFKSNKNKKFGLLKNCIFLLMLTI
                     DMSIDKKTILLKAKSLRGLTLCFTIIQELLGKLNRRFDRGLIFQAMSWKTKNGEARKL
                     PTILK"
     gene            complement(15806..16255)
                     /locus_tag="ACFB2Q_000362"
     CDS             complement(15806..16255)
                     /locus_tag="ACFB2Q_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HFC0917476.1"
                     /translation="MTQSNQNYFNLTADGIGFLNRPREVQGKRKSSNYYACTIQASRG
                     DNGEKTRFDLRIVGGQAKELFARLLEEFPQLLSKNYRERPTVVVGFRAGDIQPLKFET
                     NNRETGEVLETLSIDGRLLKFKFIKVDGDMWYQSSSQDETQNNPDAS"
     gene            complement(16519..17073)
                     /locus_tag="ACFB2Q_000363"
     CDS             complement(16519..17073)
                     /locus_tag="ACFB2Q_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HFC0917477.1"
                     /translation="MKKFVGVGALYTTGAAGLLLPKDVVAATSMADFWSRDRTINCKR
                     ADTGEKHEIRFFQQQNGYDLDAYRNACWLMRDAKDGNAMVQIDVGLLNLMYAMQEWAR
                     QSGRSNPVITINSAYRTPRRNATIEGAARNSLHMRGKAVDFTMRGVGIGELEQMAKYY
                     NVGGIGIYNSFVHLDTGRVRHWRG"
     gene            complement(17195..17734)
                     /locus_tag="ACFB2Q_000364"
     CDS             complement(17195..17734)
                     /locus_tag="ACFB2Q_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917478.1"
                     /translation="MKKLVKKLSDSIFGNTCIEYVLDENHNYKEQYNANKDLFIYING
                     IQVGSISPGELQELQYRVFYSSSLWWSIFKSILGFLLKPAIIALTALPLGVLLLLWLN
                     TDNPEAFHRLFMMLQDPAHFAKAISGLFRLFYCISFFGTFVKLVFNQDSIFSIFNKEL
                     SKNIQSNKTCSMDIVAVRD"
     gene            complement(17749..19788)
                     /locus_tag="ACFB2Q_000365"
     CDS             complement(17749..19788)
                     /locus_tag="ACFB2Q_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFC0917479.1"
                     /translation="MRLFLCEKPSQAKDIGKVLGVLGGRHDGYFQNGDTIVTWAFGHI
                     LTQAAPDAYGEQYADFGNISALPILPEVWQMQVPPKVSKQFKVIKSLLAKADEVVIAT
                     DADREGEVIAREILDYCGYRKKISRFWTSGLDTQSVKKALSSIMPGKDKENLYQAGLA
                     RGRADWLVGMNLSRAYTAAYSAGYGREHILSIGRIQTPTLKLVVQRDKEIDTFVPYNY
                     YTLSVDFETADKSVFTTLWQVPENWRNTDGYCTNLEAVKALVKQLEKSTGKVITSNKE
                     RKSTPPPLPYSLSALQKEAGKKLGLSASKVLKIAQTLYEKYKITSYPRTPCRYLPKSQ
                     MSEVKDVFAAIQTIDKSMLSVIEKADPERQSRVWNDNQVNKHSHHAIIPTKASNFDLS
                     VLDKNELTVYRMIRDRYIAQFYPDFEYDSTVVEVEACSHLFKASSQSPVISGWKVLLG
                     KDVFEGDQIDEGPALPHLKVNDEVDTLSFNPETKKTTPPPRFTEASLLDEMQTLKDFL
                     KNVEDEQIRKILKSTEGLGTEATRATIIDRLFEMGYMEKKRSKIYATEKGRNLIARIP
                     TMIADPITTAKWELALAGIEAGKLTLAEFMAYQQKVITELVGQAKKDAVGKARPPKQT
                     DSAGTKKQAVARNEDDVCPTCKEGRLRQRGFKENPDKRYWGCSRFPECKHFEWVK"
     gene            complement(19980..20411)
                     /locus_tag="ACFB2Q_000366"
     CDS             complement(19980..20411)
                     /locus_tag="ACFB2Q_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC0917480.1"
                     /translation="MSVQLFVRGNLGAEFNLRPVASPKNDEASIVLNFSVASPNFKRN
                     AEGKSEVVSTEWISCEYWNRDAAHLHKILQVGMPVFVTGEERYESYTNRDGIEVRVRK
                     LRVQDVFIVPTERVEAIKLRPKREQSQDNNHVEDADDDIPL"
     gene            complement(20714..21259)
                     /locus_tag="ACFB2Q_000367"
     CDS             complement(20714..21259)
                     /locus_tag="ACFB2Q_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917481.1"
                     /translation="MNTQISGMKKYLKFWEYKRLLRVRGIEDLEKEIKLKLVDFELLI
                     DEAQSFHEACQGLNLIYPIVREHLKLSNKSLAGLDLKKYYIPNMIFFKNVVSSSGRMS
                     RKKFFKWADISTALDDTNASLDERLLHMKVYFDCRQYFCRGRQIAGDLAELFSMKEIF
                     DEILRIENLDRKNLPSNIMVK"
     gene            complement(21355..22404)
                     /locus_tag="ACFB2Q_000368"
     CDS             complement(21355..22404)
                     /locus_tag="ACFB2Q_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcaB family transcriptional regulator"
                     /protein_id="HFC0917482.1"
                     /translation="MEFAKQRYIIKPGAIHVAQQFDLKVTTKQSPFSDGYDMARAALA
                     LGDKVNQKLTEDDEQFQQWEEFKAHELLLKQHLQKETGRKHHLVWGEYEILSSENDRF
                     KNIGSLTSSGDDGFFMHTKQGIRMWEGNNNKKNGPRWPGIRYGLTLSNELFGMAMSDN
                     PYAQSRLLQIEKQMSEIEKFFETVKKQVHAQIDSLAAAGMKITLIQNNNPVHIRLNVL
                     RAYGFKLVKLLRDYDSFVCAVKTLNIKGMMANKQCNDTLYNGGRMMRKLLNDLYIAVM
                     ETRAIKSIRRDSLLDPEQLSKLKSAVEQEILPRIPLSVWVYESQPSLVYIQRKMNQED
                     LNKLVDIVRDNGLSG"
     gene            complement(22596..24113)
                     /locus_tag="ACFB2Q_000369"
     CDS             complement(22596..24113)
                     /locus_tag="ACFB2Q_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917483.1"
                     /translation="MVTATKLPPPKVFNSELTQCWQALQGGRRVAIYRCLIDITGSLK
                     TAAMLSQLIYWTRVSKEVAERDGWIFKSIAQMEAETGLTVREQRTCKNKLLETNLIQT
                     CRKGVGAALAIKVNLDAIAELIAKSSGTDDQLALTLADLQNTSSLYFRKHFSKRIAYH
                     RDLVSITGCINSAVLLSCVLNDAVGLVSQNPHLQRHAFATLTAADWEERTSLSYKSQL
                     TARNRLKNLNLIYERNFLASRRIFTLVNGHDIVISIKKLLAGKQDDGFSPYNSKKREI
                     LSLAERAKCDWRKGPNGLDKGFQSGFEGDAPLFPSMTSGSVEREYSDVSNWNFKKGRS
                     GKFISVDWGSSELPNRQIIYYINYKGVYNYNYMTVEPNKPRSSEQDHVVVVDDVDLIY
                     PKLFNVQMRQQADLIFTKHLSKSSWALRQEILDEIQGQKKEVTSPLGLLINLCRCASQ
                     GNFTALVAPQVRFQREQDQKLQAYLEKQNNQKQDSLDAMDVELARNNLRKFYSKR"
     gene            complement(24113..24856)
                     /locus_tag="ACFB2Q_000370"
     CDS             complement(24113..24856)
                     /locus_tag="ACFB2Q_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917484.1"
                     /translation="MDFSTSYDYETGFVPSKNMSTDNFIRSLVALWEEPATYVRQFAQ
                     AVGSVSEGYFLSWFVEKCLPGEWVSLNNDLLFNEAGITAKQWRNIRNSLLQKGILLNR
                     RTVAPTESQFTLNDALFEDLVSKHGKSDVLDMVSPPISINKLHLKTLADRGLAFVSIL
                     YLSFLQSEAEYQDLSKRGDWSPWTHAAEKHVFYSTMLSRRQQERAIAELTSFGLIESC
                     VDGMPAARRCRYSLKMLADLTSEYLNGCV"
     gene            complement(24865..25470)
                     /locus_tag="ACFB2Q_000371"
     CDS             complement(24865..25470)
                     /locus_tag="ACFB2Q_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917485.1"
                     /translation="MRSKPLISNPIYRLFNTDPVYTNLALTRLASAPNTPRESSYNAL
                     VAREHEDVLNTLKRMNPKRIEKLASMCPDVVTYSIDLKVLKSKISEMEHYEQRFMEQM
                     QRLKWMLEHKASNLMIMNLCSLISTAEIKKLRIEMNIPVVKGRIVMPSIDVRVRVLKA
                     WSKSEKEPDLFIRYQLLIDEFPDYSLAQLHSIINDVKFFGV"
     gene            complement(25460..26950)
                     /locus_tag="ACFB2Q_000372"
     CDS             complement(25460..26950)
                     /locus_tag="ACFB2Q_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFC0917486.1"
                     /translation="MNLDQNKATLSLHIGTPAQTNMSFGQEDTPSRGMLMSLPISEID
                     FFDKNPRRLHDVSSYAAIKESIRSSGIQQPVHVTQRPGESKYVLAQGGNTRLKILCEL
                     LEETGDNRFRMMPCIYIPYSDETTLQVAHLVENELRAEMCFWDKACKYAEIRDIFQDE
                     SDSGKLSLRELEVMFSDKGLTVSHKTLGLFFFASDNLQELQEYAFKLSHAKVEEIKKL
                     HSTLFSISKSIQLDDSFQYLWSDVLEQWHNQYSATELDVHELYQFVLDRFKFEYGSDA
                     FSETENSSDKDKIETAAKQDVNLHQKDSDMEFVPAKVNGTKADAEDEPKEAEPITLEQ
                     TSKKLFSAVRKLLAKVSLSDCFKIHKDFPYGWYVEYPAFEHISASSAGNAIYAIDVLH
                     EEAGNVFTFLWKLSGIDTFMFDVGDSSNPLLTLKSDSKLRIAYEDPDKYDEYTNCGIG
                     NKENIIASLLNWQTNTSHEFHANIVEILSLSTQYNNLRKTGAKSEE"
     gene            complement(26947..27834)
                     /locus_tag="ACFB2Q_000373"
     CDS             complement(26947..27834)
                     /locus_tag="ACFB2Q_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC0917487.1"
                     /translation="MSAPVILSAAATKGGVGKTTLIANVSAVLADIGLRVLMIDCDVQ
                     PSLSKYYPISHRAPNGIVELLLGENTEEIIRSTISNTVFPNLDIVISNNISAFVETEV
                     TSRPDRAFLLKSKLHSQYIQNNYDVVLIDTQGAVGPLLDTAAFAATCLLCPIMPEVLS
                     AREFLTGTQELLKRLQKGEAMRIPMPQLRAIIYAQDRTNDAKFIASSIKDFFSNTLDD
                     RRKLLSVAVPAAKAYKEATTLRVPVHCHERKQLGKMLPAYEIMHRIVYEIFPGIEQKN
                     LRGSCFNDMASLLQKEVQS"
     gene            complement(28075..28257)
                     /locus_tag="ACFB2Q_000374"
     CDS             complement(28075..28257)
                     /locus_tag="ACFB2Q_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917488.1"
                     /translation="MGVCPDARCSAAGIARQIGNGGGIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPLFLLI"
     gene            28257..28592
                     /locus_tag="ACFB2Q_000375"
     CDS             28257..28592
                     /locus_tag="ACFB2Q_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917489.1"
                     /translation="MPNLVTAVLAGRFLRWRIPYPTHQTDNISSRILTARICWYESSA
                     QAEKAVAPKTVARINCLNFMMLLLLHKAFGIVKPAAYGFAAVAALQAVLQETLKHDHC
                     IKSCSNSAK"
     gene            complement(28629..29288)
                     /gene="ung"
                     /locus_tag="ACFB2Q_000376"
     CDS             complement(28629..29288)
                     /gene="ung"
                     /locus_tag="ACFB2Q_000376"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC0917490.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            29369..29776
                     /locus_tag="ACFB2Q_000377"
     CDS             29369..29776
                     /locus_tag="ACFB2Q_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC0917491.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDTGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            29791..30738
                     /locus_tag="ACFB2Q_000378"
     CDS             29791..30738
                     /locus_tag="ACFB2Q_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC0917492.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(30845..31915)
                     /locus_tag="ACFB2Q_000379"
     CDS             complement(30845..31915)
                     /locus_tag="ACFB2Q_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC0917493.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FIQNTLLPWLKNTIGGYVEIDQASIIAWFQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            32100..32375
                     /locus_tag="ACFB2Q_000380"
     CDS             32100..32375
                     /locus_tag="ACFB2Q_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC0917494.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            32377..33000
                     /gene="lipB"
                     /locus_tag="ACFB2Q_000381"
     CDS             32377..33000
                     /gene="lipB"
                     /locus_tag="ACFB2Q_000381"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC0917495.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            32993..33976
                     /gene="lipA"
                     /locus_tag="ACFB2Q_000382"
     CDS             32993..33976
                     /gene="lipA"
                     /locus_tag="ACFB2Q_000382"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC0917496.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
BASE COUNT         9626 a         8211 c         7258 g         9146 t
ORIGIN      
        1 tgtgattggc agcatttcag ctaccaatcc tctttcttat atgatacaca tcccattatt
       61 tataagttac ttggatgctg tcacttgtaa tccaacgttc gctgcctctg ttcggctgaa
      121 ttttaattaa gccattctca cctttttcta agaccaaggc agacccatag gaagtggaga
      181 tttttttacc gggttgtaca tccgtaactt tgctgcggta ggcttgttcc cctgtatatc
      241 catcagagaa tgcaaaattc gtatagtttt ctctgtacca ccatcccatc cactggactt
      301 gtctgccgtt gatacagaaa atatcggaag tcagggtttc aaccgtcccc tgatgatgtt
      361 cgcaatatgc tttgaaagtt cctactgcat cctcaaccgt tttttcatgt tcggattcgg
      421 atcttgcata gaattttcgt tcaatagttc ccgtactttc aaaaatcttg ccttggatga
      481 tacggccaag tcccgccaat ttactgtctt tcatccaatc ttgtacaaac ttgggatagg
      541 gtttgtctaa agaagcttcc aataccccgt caattttggc cggctttgaa gcaagattca
      601 agaaattacc ttcagcttgg gcatttgaaa tggccatagc tgctaataat ccgaacataa
      661 tttttttcat tgcaatattt tcctcatctt attgaaactt agggtttgtt ctaaacagtg
      721 gtttatgatt taaaccaact ggccatgcgc tgcagaatcc catgcttttt cggaatttcc
      781 cttttcccgg agtgagcttg gaaattctga tgttctaata aggctgcgta ttgggcggcc
      841 aaagccgaat attcagcttc caataaaaca tgtttattcc tcagcgcctc aaatttttcc
      901 tgtatttgct ctgcttgttg ttcatcggcc ttaatttgat tatgcaacat caaggccgtc
      961 tgaaaatccg cactcaaagg attacggagt agggtgcaga gaatttgttt ccgaaaggtg
     1021 ttccgaagtt tctctacatg gcgatttatg cggtttttga tttcttcatt atcataattg
     1081 agcagctcgt cttcatcaat atacaaaaat gaacccttat ctgcccgtat ctgaaccacc
     1141 gggataccta ttttcgataa ggctaaacat ttaggcgcat tggaagccaa ccgtgatgta
     1201 tgccaaatct caaccgccaa ttttctctgc caccttatct cgtgcctgag cagggaagtg
     1261 ttacccggct gatcaaattc aagcaaaaca tcaatataaa aagttttgcc atctatatct
     1321 atccgatatt cggataatgc acggttcagc ctaatagtga caggcggttg ttgctcggcg
     1381 tgttctgtgc aatttagaac agtcgtcagg ccgccgggta agttggatag ttcgttcagc
     1441 acttgtttgc aaagcaagtg ggttagcgat tcaggggcgg aagcaccacc ggagtcggct
     1501 tcactgtaat agcgaaaaaa attggtcgcc ttcctcctgg tcaaaaccat tttctttctt
     1561 gtaccgggac gatgacggct gtagtagatc attccttccc ggtgttttag ctttgcttcc
     1621 agcgcagtta cccattcatc accatccgga ctcgtataag caataatttt caaacgatta
     1681 aaatcctttg ggaagaaaag catcatcaga ataagacaac aataaaatcc tatatatcct
     1741 gtttgccgcc aaagggcaat ttttcttccc ccttttagat ttgtccggct catatacgga
     1801 catgggcaac attatagata acagcaatta ttattacaat gttgtaacat tcagtctaat
     1861 actgtatttt gaccatgaga atcttctaag ggctgttttc ttttgctcta ttcctttgat
     1921 actacttcaa gcagtgcagt ccaatctgaa ggaggatttc cgttccccag catttttttg
     1981 aacacagtat atgcatcatt tttgccgcca taagttcttt tcgtacccga gtcgtttacc
     2041 catgcgagaa tgatgatttt actgtttgag tcgtaacgga aaaacaatcg gtatctaccc
     2101 ataaacttgg cacgtcgcca aaatttgtat tccgggccga gtgtcttgcc aagccgatat
     2161 tccgggctat ccggattaag ggcaatattt tctatttcgc ctaagattcg ttccagcagt
     2221 ttggcctctt cggatctact ggatttttcg gatcgtttct ggctttctat cgtagtggcc
     2281 agtctgtcta attgctgcgt aaagcattcg tgttcgtaaa tggtatatcc gtttatctgc
     2341 ataggggaat caggcatatt agtctttcat atctgtttcc caagtcagtt ggcgaaggcg
     2401 gttaaagcgt gattgagatg tgggtttgat ggaattcgga ttttttgtca tagaattttc
     2461 caaaaaatgc aggaattttc caacaactgg atcatgctca aattcttctt cctcagtatt
     2521 gtttttttct agcaatacct gtccgccgcc taagacggaa aaacggattt tgtcctgttt
     2581 ttccagcccc aacgccttgc gtactgactc gggaatggtt gtttggtttt tgctggtcat
     2641 agttgacact gcatcaaaaa taatcttcat ggtctctttg ccctgccttc attcaaattt
     2701 actgttgcta cttgcgactt caatgtccta gttacgcaca tcttctactt aacggcttgg
     2761 gaaattattg atattgttaa aaactgctta aattaaagaa ttctgtttat tgtaaggaaa
     2821 tttccttaca gggtcaatac ccaacccctt cgacctgcaa gggcgtttat gaatacccat
     2881 ttactgactg gcagcgatga cacgcttaag tgagggagca ggcgtaggcc ggaggtaatt
     2941 cctgttcccg agctgtcggc ggcgagaccg ggggattggg ggcagagacc ccaaaaatat
     3001 cagagtatgg gcacaaaaaa acggccgcat tagcggccgt tggttttgaa aaagattatt
     3061 tgaggcacaa atgctacttc aaacaggcat cgagcttgga ttcaggatca cactctccga
     3121 aaaactcatc caggaacgct gataagtcca gttcatacaa cgtccccgtc cagtcgttat
     3181 aaaactccaa ataataagag aacgatttga tagggaaagg ctttacatcc aatctttcgt
     3241 atctcaactt tccttcgaaa ccgaacgaat acgcactgca ttttgtgctg atgccgtgca
     3301 aaatgccctg gttttcgttc aaaagtctaa cagcagtgtg aagggattca acatcgatgc
     3361 tgacttctaa gtcgggtacg gtaaagtcat actcgtccgt ctgccaaatg gtatttaatt
     3421 cgtcactacc ttcgggcagg ttaaggtaaa ggaaacgttt gccgttttcc tgttttagga
     3481 tgaatcggcc ttcgcatgaa gcctgagtga tctctttgtc aatctgattt tcaatcattt
     3541 ctaatctcca caaaaaagcg ggggacaacc ctgcgggttg tccccgcagg gtgagttggc
     3601 aatacgtcta acgaaatcta agttctttaa tcttcgtcat cgtattcgca tccaaagtgc
     3661 tcggtagcga agtcacggac tccatcatct gtgcacggat aatcgaacaa tgctccttca
     3721 tgaccttcat aggttcctgc aaaccacatt ccttcttcca ggtatttggc ttcaaattct
     3781 acgtcaggaa aacgtttggc gagttctcta taaatccctt ccggaggacc ccaggcagtg
     3841 tcacagctga tccggatgcc gctttcccca actgtcacaa aagtgcagtc ggggtttacg
     3901 tcccatttgc agccccagtt agctaaccgc cagtcatacc aattcggatg accgtattgg
     3961 cggatgtttt ccgcaatacg cttggcaaat ggttcatcgt aacggaaacg agatgcgact
     4021 tcagagttag cttcaaaatg gagaaatact tcaccaacag tcagattgtc ccaatcaaat
     4081 ttttccgaaa aatatagctt taattcagag aaaagttcat ccggcaatct atattgaacg
     4141 gaccaaaagg atttatcgcg ctgttccagc atttcccgtt ccaaattgat caacttcttt
     4201 tcaggttgtt gagcaatttg aaaccaggtc tgctgcatgt aactatcagg cacttcagga
     4261 agaatcagct cctccggcat gggtacaata tgctgaaagc tgaattgata ccccttgtcg
     4321 gttttgatca gaatttgctc cgccacttcg gcgatacgtt gaggattgcc ttgaaaatga
     4381 aggctgttgc tgcaccagtt gggcatagta gtctcctgag caggaatccc catccctgcg
     4441 gataaggaaa cccgcaggtt gataggcttg cgcccgggtt gataaaaaag ccgccaccgg
     4501 aaaccggcgc acatttcaga cgacaggtgc tttagataaa aggcaaagaa accgaaacgg
     4561 taactttgcc tttgggaagc ggaacgacgg ttccgccggc ttgatgaaaa atgtgcttaa
     4621 gcacggttat tttttggggg ttttaagata gattctcgca acaataacaa gaaccaggcc
     4681 gactgcggcc atgacccaag ttccggtaac gaagtccatt atttgtcctc cagtttagag
     4741 atgtgaatgc ggataaggaa tgaaacatac agcatttcaa acgacaggag caccaatcct
     4801 gccatggcaa gattcttcat ctcctcacta tatccgttac tccagaccac cacaccgatg
     4861 acggggaata catccccttc agggttgata aaagagccgt gcatccgaaa cggatgtgca
     4921 tttggttaac ggcggtatgc gggcggtttg acggactgcc taccgtgtaa catagtctaa
     4981 tcttctacca aatacccgtc gctttcaagt ctatcggaca agccttcgta ccaaatatcg
     5041 aattcggcga taccgaattt acggatgtcg gccatctcgc aaagacagtg gtaaagcacg
     5101 ctgttacggt attcgtagcg tccgccgaaa cttccgtact ggttgatgta tttcgctgta
     5161 aaacatacat ccaagttggc gaagatttcc gcgagtttgc gataaatacc gaaaggcggt
     5221 gccaatccgg ttaaaaagca gatttcaata aaaccctctc tttgtgtaac aaaggtttcg
     5281 gtgggatatg gggaaaaact gctgccccat gcctcgattc tctgtttggc aaggtcagac
     5341 gtgatatttt cgggtaaagg caaaatccga ttgaaatcaa aagcccaatg ctcaggatga
     5401 acgggggagc gaaataaaat cagtttcgcc gcttcggcaa ccgattcttt ggttccttct
     5461 attagaagga tgttgtcaac tttgatagac ataactatct ccagtagagt ttctgcccct
     5521 gcggccaaaa gaccgcaggt aatgggcatt tacccagttg gaaaagaagc acggcggtta
     5581 tgcctacaca ttgtacagct cgggcaggat ttcgcggttc agccagccgg tgacgacttc
     5641 gcgcagcaca tggcggcagc catcagtcag aacatcagat agactgaaac cgaactcgta
     5701 cagccccagc ccttcattcg actgccactc gatgatggca ttgatgtggc ggcggaaaat
     5761 ccaggcaatg tcttcatctg ataaatactg caatgccccc tgaaaaataa ccattttgtc
     5821 aggacggttg aaatactgcc gataggcagg catcagatag cgcatgtcgg caacggcgga
     5881 tacgccttta ccggcgtatc cgtcctcaag gtaagtgacg gctgcaacgg catcaccatt
     5941 gctactgctg atgcaaacca tgtcggcatt agcctgtttg gcaaactctt cacaggtttg
     6001 ggcaaagacg gccaaatcag cccctaatcc aaatcttttc agttcacgtc taatagcggc
     6061 catgacacca tccgtgtgag cggtcaaaac tgcaccgttc aatttagctg ccgcagctgc
     6121 cgattcttcc cgatcgggat tcatccatac aataaagttt tgcattgtgt tgctccttaa
     6181 aaagaaaacg gagaacacag tccgtaggga ttgtgttccc cacggggttg ataaaagaaa
     6241 atgccgtaca tccataagga tgcgtgtcat gtttaacggc aggacacaag acagataagg
     6301 tctgccagcc tgtaaagaca ggtattttca gacggcctta ctaaagccgt ctgaaaatac
     6361 ctgcccgaag gcaggtgaga tatataaaaa gattacaagg tcagtaatct tctgggaaca
     6421 ggagtgtggt aaggcttctg tcccattctg taacaaccca aattttttgg tccccaatct
     6481 tgtaagaaga cattattctg ccttcttcac ctggcttgag tgccagctga ttcggcttgg
     6541 catcctcagg aggaatatca ccccatatgc catggcggtg ggcctcaagc aatatttggg
     6601 cataaagact gttctcatac agttgtgcag gcaggtgctc ttttgcccgg taatctactg
     6661 ttgccgtcat agacactcgg cccaggtcaa acagagaggg ggtatcacat gcggccggag
     6721 ctgccgaatc aagttgttcc attaggcgta tccttaaaaa aaccggatac acccctgcgg
     6781 gaagtacccg gcagggttta aaaattgaat aggccaatct atttgattgt caggactcgt
     6841 ccaaaatccg acgatccttc ggtcaaatca attgcgcgta tgcttggaga aaaccggtca
     6901 gttgaagtaa tcgtgaccac aagcctgtcc ttcttgccgt actcttccga tttggtttcc
     6961 actttggttt tatgggttga acctttaacc agcagctttc ggccactggt tgaggtgaca
     7021 acgccattaa cttgccctgc ggctaatgct aaagcagcat gccactgacc cagagcacat
     7081 aaaggccggc gtttttcggc tataacggca ctggtgccga aatattgcga aaatgatggc
     7141 cataacgtgc gggattgctg ctgtcccagt tcacgttcca atatatcctg ctccaattta
     7201 aagcttctgg ggcgaaagac cttattgggt gcaataggca cacagtatgc aggcataccg
     7261 gccggcaaag tctcaggggc attttcaaaa tcaagcagga gcctttgttg ggcatccgcc
     7321 tgttttttgc tgatagaggc tttgttcttc ggtcggatgc cgaatataac caactgtttg
     7381 taggtatccg ccgcagccct tatcatacgg acatccgtac agtgggtagc gatttcgccg
     7441 gttagatgct ctgtcagggc ggcttccgga acaatcatta ccaacactcc gccgggctgc
     7501 aaagtaccga aggtacgtgc gaagaaaagt tcttcaagcc gtttggttct ttgattagac
     7561 agattgtctt tggcggcaaa gccgtaagga ggattcagaa acaggaggcc gactgccgaa
     7621 ccctgcatgg tgcagttttc tacatcggat ttgatgacac tggtcagtcg gctcattgcc
     7681 tcttgggcac ggtctttctc cagttcaatc ccgtaagttt gacaacttat tttttccgat
     7741 tgcaaagtag aagctaaaga agccagggct tctcctgttc cgcagcaagg atcaaatatg
     7801 cgtaacaaac ctccggcaga tgccggaata tcaaggcaag cggctatacg cgacaatgtg
     7861 gcctggtcgg tggggaagta tccgttgtca cggtggttat gcgcgacacg ggggtgcatc
     7921 agatggtttt gagtttccat attggtctcc attcaaataa aaaacggaga catgccgtgc
     7981 gggacagtcc ccgcagggtt aaatcaaagt ttaatcgaag gtaccgccac gttctatatg
     8041 acgtaccatt gttttaaaat tcagcaatcc acgcggatcc tgtcccggcg agccgggtat
     8101 cggaattttt cgatcggtcg gtaggtgctt caatgcgctg tgcttacgac cgtttttggg
     8161 gaggaacatc cattctcccc ccgataccag ttggcgtata aaaaattgat aatctttgtt
     8221 tttgctatat ctcatttctt ctccgataca aaaataggtt ttcctcctgc aaatggttaa
     8281 aaatacacgc atacgattaa aaaaaacgta accgcatgac ttccaagaaa aatcccactg
     8341 ccgtacagac gtacggaaca gcgggattgc ggcttcaagc cggatttatc taaaaaggaa
     8401 cgtatcagcg ttctatttaa aatgctttgg tattaaatag caacaaagca tttaaaagat
     8461 gggaatacca attaacttga ccggtcatgt gtattagtgt cttgctgaga ctcgccaaac
     8521 tttaccagtt taaatatgcc aaacaacatg acggcaatca aaaatagtaa gctgtaggtc
     8581 atgatcatgg tttttccctt tccttgggtt cctgtttgac aatctttgat tcatatttat
     8641 ccaagtaaaa gataaatact tgagctacat agaatgaacc gcgttaacgg tttttgtgtt
     8701 cttccgacgt acgcgaagtt tcaagctgct tggtaatcca atagccgaaa gcaagcacca
     8761 ggaagagaat aaatagcaga ccgcctatca gcatgacgta tcctttctgt ttaatgaatc
     8821 acggtatttc cgtagtttgt cagcatatac ttgcacaaag taggcaacca aagccaatac
     8881 ggctacgccg gaagcacgtt cggtcaaaga gtatgaattg agccaaaaca acggcagcat
     8941 tgccaacaag gctgttttac ctatatcttc cagcaatttg atccaagatt caatgctttc
     9001 cttgtgaaaa ggaaggtcgg gtttgatgaa tataaaaaat ttcacatcgg tatctccatg
     9061 aaaaagggag atataccgat gccctgtggg gatacggtat atccccacag ggttaaagaa
     9121 agttgaaaaa gagccgtccg tcccgaagga cgtgcgcaat gggtaacggc agtgcgcggg
     9181 cagataaggt ctgccagcct gtgaaattcc accttacggg ctttttcaga agacctcaac
     9241 acgaggtcgt ctgaaaaaac ccactaaacg cgggttagat gcaaaaattg catcaatctt
     9301 gtcggtcaac gaagcgtttg gccaactgaa cttcaactga atcttcagcc tgattcagaa
     9361 tgtccagacc cgaatcgggc atatcaccgg aggttgactg agtaacggca actttttgcc
     9421 ccatcagttc aaggcaaatt tgctgcgctg tatcgctgta accggcgaaa tacacttcca
     9481 cgtcttcctt ctgaccaata cgccaagaac ggcgggcagc ctgcatcagg gtataaacgt
     9541 tatatcctgt ttgcaaaaag tagatggttg gaaatgccaa caagtccaaa ccggttttga
     9601 ccagctccgg attacagata acaacctgta cgcctgaagc caattggtct tcaacccagt
     9661 cttcgcgttg atcggctttg accgtcgctt tcattaccgc cgccttaata ccgtgccgtt
     9721 tgaaaatctc tttcagacgg cctgtcgtat cacgggtatc ggtataaaca gtatagacca
     9781 gacatttacg tcctttagcc agactgtctt taacgacttc cagcatgtca gcttctttcg
     9841 gtgagatttt ttcttcatcg aactggggtt cggtatggaa aagaatcttg ttttgtctgc
     9901 gccagtaaac atcttgaggt ttgtaacaac aatccggcca tgacagcaag gcgctcataa
     9961 caacgccggt aagggtattg tcacgattcc ttaatgccac cttcaaatgc tgcctcaaaa
    10021 cactctccat atttttatag accgcctgtt gatcttcagt catttcaacc ggccggaaaa
    10081 cttctttata gccaggtaaa acgccttctc ccaaatcacg cagcttaaga aatacggtaa
    10141 taggcaaaac ataacgcatg atgcccaaag gggaaaatcc cggcgccttg gcggtgcgaa
    10201 cgctgttacg ctctgctttt gagctactga atgcgccatt actgtattca gttccaagat
    10261 gtcggacaat atccttcaaa acgccatgct tacgcataaa cgccatgctt gccgaaccca
    10321 aggtattccc tttgttgtaa ctgaaaccgt cttcaatcat catttgcggc caaagccgcc
    10381 acaatagaaa gaacaggtct tccgcataac cacccatcag cgtaccggtt aagcacagga
    10441 tcttattaca gcaacgtgcc aaaacgccca tggcttgacc ttgagccgta ccgtagttct
    10501 tgtactcgtg accttcatct actaccaaca gcccgaaaaa gttcttcggt aaatagcgtt
    10561 tgacgaactc ggtcggctga tacccgcctc gtcccaggct gaattctgct ttgcctaagt
    10621 ctcggtcgat acgcgccgcc tgacggtcgt cgaataaaaa ttctccgttt tcatccatca
    10681 aattggaaaa cgcctgaatg ttgttttcca agatttcagc cagatactgc tcaccataca
    10741 tcccgatgat ttttgctgcg gttttaggac cgatgccggg tagggtggtc atggttttaa
    10801 gtacgcgatc gtacactgtg cttacaaccc cgtcttcact ctgttccttg cggcacaagg
    10861 tccagagcgg ttccttacac ggtttgccat gatgattgcc tttgcaaaaa cggcggtttt
    10921 tagccagggc gtttttcaca ccaccttcag tgttataagg cttgttttcc tcatccaaga
    10981 tttcacagcc acatttagga cagtaatact cggaaacggc aaaaccctca gaagcacctt
    11041 cagaaggtat ccggcggcgg cgaacggcat acgaaggcct ccaatggtag cccatgcgca
    11101 tccgaacacg ccccatcaca aaaaactccg gaacttcggg tttgcggcca agggcacgga
    11161 tttgcaacag ttttgccaat gtatcggctc cgttaagaat ccatacgcgg gcattgggta
    11221 ccgttttgag gatctctcgt ctccacttgt acaccaaatg aggaggactc aataccaagg
    11281 tgcggtgcaa acctgcttga tgcgcggcag cggctgctgc aaccgacatc atggtcttac
    11341 ctgtgcccat ttcggcatta ataatggctg cgggtttgtc ctctgccacc aattgctgta
    11401 atacggcgcg cacaacttcg cgctgcgccg caaacaaagg gcgttccagg ttatccagca
    11461 cttcttcaag gagaggataa gtttgtccct gatagaccgg ttggttttgc tcagtaactg
    11521 cgttaaagag actatcccca aactcggcaa gaaactgtgt caggggtatg tcggaatccg
    11581 gcagttcagt ttccggagat tcctccggca atacggcagc ttccgtactt ttttgggcag
    11641 tctcagccac ttggcggatg ataaagttga aatcagtagt tatttgattc tgacgttcct
    11701 gcatctcggt cacagtttga tccagccctg caaaatccgg gaggagttcg tctatttttg
    11761 atacggggtt atacatcgta tgctcctaaa aaaaagacgg agcaaacccc ggcgacgggg
    11821 gattgccccg tcagggttag aaaccgctac cgtatggtgc ggaaaaaggg gttagcgaag
    11881 attcgccaaa ggaaaaaata gatgcttggt ggcatcttcg aaagaagtaa agccgaaatg
    11941 cctgtaaaag ccgatggcag cttcgtcttt tgcctccaca atcaacgcat acgcggcgat
    12001 ttctgcttta ccgatacgtt tgagtgcatc agccaaaagt aaagaaccca gtccaaaccc
    12061 gccatatttt ccatctaccg ccaaacggcc catcagcact gcgggaaccg tagggtatct
    12121 gggcagcttc ttacgaaaac cggcgggtat attacttagg gcaactcctg ttgcagccaa
    12181 tgtatagtag ccaagaattt gaccgtcgga tgtaaccgcg acaaaacaag tagcgacttt
    12241 ccgttttacg tcttgcgaaa cctgtttttg cagatagcgg tttaaaggtt cggaagggcg
    12301 ctcaaatgca ttgcggtcat cggcagaatc aaggaggcgg attcggaagt ccggtaacgg
    12361 cattttatct cccgaacaat tcgtcatgtt tttccaatgc tctgctcaat gccccattcg
    12421 gtttggacgg attgagcagg gcttgggcaa actgaagttg gtcacgttcg gacagcatca
    12481 gaatctccgc atcggcaact gctttttggg cagcctcgcg ggcagcactg atgacaaatt
    12541 cggacatact gcgtccttga agttccgcag cgtgtttaag taaagcacgg gtatctgaag
    12601 tgattcttgc ttcaaaacgg caatcggcat atgtggcagt catgaacata ctcctaaatt
    12661 atttctagat tgtacggctg attaccgtac aaaacaagat atgaaagggt attttgtaga
    12721 aaacatcaca tgctccaaag taacggagat atacgatctc catagggatg tatatcccca
    12781 tagggttgag tgagaaatga taaaaatccc accggatgcc ggaacatggt gggattgaag
    12841 gttacagata aaaagctccg tttaacggaa cgggtttaat gttacaggaa cttcgcggca
    12901 gcaacggcaa cggtagtcgt gataccgata aaccaataaa ttgcattgat tttagtatcg
    12961 attgcgctaa accgtgcatc tacgctattt tcaaatgagc tgatgcgggc atctacacta
    13021 ttttcaaatg agctaaaccg tgcatctaca cttttttcaa atgagctgat acgggcatct
    13081 acactttttt caaatgagct gatgcgggca tctacacttt tttcaaaagc gtccagtcgg
    13141 gcatcgaagt tctgggtcat gaaatccagt ttgttcaaaa ccttttccat gtcgtcctcc
    13201 gttttcaaat cgattaaggc tactatagca gctgcatcct gacggttcaa gccggacagt
    13261 acctcatgct tttcagatag agtaggcata aaatcctcct aagtttggga atatgcctcc
    13321 ccatgggacg tatatcccca cagggttggt aaaattgaaa aatgccgcaa ccatacgaat
    13381 acggccggat aacctcagca gcaatatcca tcaacggaat atacagtccg tcatctgtgt
    13441 aaaactctta tgtaacagac tttttcagag agcctttgca aggccgtctg aaaaagcccg
    13501 ctaggggctt cagattaagt atgtaagcgg ccgctttgta gcatagcggt caaacgcaca
    13561 tcaaaatctt ccgggatatc aactcgtacc gcttgaactc caactactgc gtactcttca
    13621 tctatacgag gagtaaaccg catgatggat ttatcagcct caagtaccga taaaacagca
    13681 ttctgccaat gttccaataa cggtatatcg gagagttgcc gcaccagttg ccaagctgcc
    13741 ttgtccaagt ctgcggtaac atcatccaac ataacccaac ctgttttact atcgaagtta
    13801 ggttgaatca gaatatccga atacaaaaac atgtgcgtga ccgaaccata attaagagtc
    13861 tgatatttcg tcatgtattt gctcagattc aagcaatgct cggcagtcgt caggcgcgga
    13921 cagcttaaaa cctcctccgg ccgtctaaaa ccaagccgtt cattacttac ttgcagttga
    13981 gcttggaagg acaacatgtc tgcatcgcgg ccgtgcagag aagcaaacat caactgtcgg
    14041 ttccaaatga agaaggcatc ggccatcaaa tcattgcggg aagtcaggta aagcattcca
    14101 acctcctatt gcagacacca agtctgcgac tggggccgat aggaataccc tgcctccttc
    14161 atcattgaac gggctatgtt cagctcgtca cggtctgaaa ggatatactc acccaaactg
    14221 atatgctccc ggttttgggc gatcatttcg ccgacctgat ccgcagtttc cgcgtattca
    14281 ggcaaccgga tgccgttttc aggaaccgga tcgaacggat cggatggatc atcttgcgga
    14341 gaatccgacc cggaagaaaa ttcctgcgca aattccgcca gatgttgcca gtcaagagct
    14401 gcatctaagt aagaggtggc gccaccaccc cactcataag ccttaacttt gagctgttca
    14461 ataatgaagt ttccttggta ttcaccaggc tcaaactgtt tcaatattgg tttttttaca
    14521 tcaaatgttc caattgatgt agctaattta gctgttttga aagggcctcg gcgaccctgt
    14581 atgacttgca ccgtcaaggt gccggaaaga ttgatggaca tggtatcact ccaaaagaaa
    14641 agggataccg gccgcgggca gatatcccgt ggttgggaaa aaacaaatta gcgcttaata
    14701 gcactgtaaa tcttatccaa atatactttt tgggacatac gcagtcccaa tcttgtaaaa
    14761 atacgtttgt cgttatccaa tgcccgaata gccatattca acgctacgga agcgttttta
    14821 tggctatgaa catcgttgac gctcctacaa taccgattga ccgccttttg ggttcgtatt
    14881 ttcgaaatac ggccatccgc atcaacaagt aacaaatgat gttcttgtat gcatgagacg
    14941 acaactttcc gatgagtatg tcggtgggtg aggatgtcac cgatacgtat tctttccggt
    15001 agtaaatttg ggggaaggat gacacctgaa cggtgaaaag atagatttag attcatggta
    15061 atactccaaa ataaagggta caccatgccc attcggggaa gtgtaccccg atgggttaaa
    15121 agaaatctgc ttcagaaaaa agcagctgta ccgtacctga aacaggcttg caatcaacgg
    15181 ctatgcaatg acagaaaaaa cagtctgtca gctgtataaa actcttgaaa tacagattct
    15241 ctgagccgtt ctgcaaatat caaaacgtct tagagaacct gcaaagcagg ttcaaatggg
    15301 gattaccctg gctaatgatt acctagtccg caccgacgaa gtaatcagat aatggtctgg
    15361 atagtaataa aaccgtaaca attacgaata attgcgcgca ctggttatac ggctatacgc
    15421 agacagaaga aacggtctgt caaccgggga agcttacctg aatgacctat ggtagggagg
    15481 ggcattcagg taagctgccc aaagggcagc cttcaaacag ttgcttcaaa agcaacaaaa
    15541 ataaaaaatt cggattgtta aagaactgta tttttttact gatgctcaca atagatatga
    15601 gcatcgataa aaaaacaata ttgctgaaag ccaagtcttt gaggggactt accctttgct
    15661 tcactataat tcaggagttg cttggaaaat taaaccgccg tttcgacaga ggcttaattt
    15721 tccaagcaat gagctggaaa actaagaacg gggaagcccg aaagcttcct accattctta
    15781 aataatcaaa atattcaata gctttctaag aagcatcagg attattttgg gtttcatctt
    15841 gagaggatga ttgataccac atatcgccat ccactttaat aaatttgaat ttcaacaacc
    15901 gaccgtcgat acttaaagtt tctaaaactt cgcccgtctc acgattgtta gtttcaaatt
    15961 ttaaaggttg aatatcaccg gcacggaaac cgaccacgac agtcgggcgt tcacggtaat
    16021 ttttagacag caattgcgga aattcttcca aaaggcgagc aaagagctct ttcgcttgac
    16081 ctccgacgat acgaagatcg aaacgggttt tctccccatt atcacctcgg gaggcttgga
    16141 tcgtacaagc ataatagttg gaagattttc tctttccttg aacttcacgt ggccggttta
    16201 aaaaaccgat accatctgca gtcaggttaa agtagttttg attagattga gtcatgatat
    16261 atctccaaaa aaacagggga tatatcaatc ccaaagggga cagtatatcc ccaaagggtt
    16321 aagttgcggc tttcacggta gccgaactga cttagaagcc ttaaaggctg gctgaaaaag
    16381 aagtaccgta aattcgacga atcgaatctc attcatgaac ggctaaatga cgagactttt
    16441 aagaaaagtc acaaactaca aaaacgatta caatatattc ccgataattt cgtatgtcaa
    16501 tattttttcg taaaatcttt aaccgcgcca atgccgaacc ctgccggtat ccaaatggac
    16561 aaaactatta tagataccaa taccgcccac attgtagtat ttagccattt gctcaagctc
    16621 gccaatgccc acccccctca tagtaaagtc aactgcttta ccacgcatat gaagtgagtt
    16681 tctagcagca ccctcaatag tcgcatttcg tcttggcgtt ctatatgcac tattgatcgt
    16741 aatcacaggg tttgaccgtc ctgattgccg tgcccactcc tgcattgcat acatcaggtt
    16801 taaaagccct acgtcaattt gaaccatggc atttccatct ttcgcatcac gcatcagcca
    16861 acaggcattg cggtaagcat caagatcata tccattttgt tgctgaaaaa aacggatttc
    16921 atgtttctcg ccagtatctg cccgtttgca attgattgta cggtctcgtg accaaaaatc
    16981 agccatactg gttgccgcta ctacatcttt aggcaataac aaaccggcag caccggtcgt
    17041 atatagggcg ccaacgccga caaacttttt caaaaaattt cttctatcca tctgaaattc
    17101 caaaccaatg actccaatca aattttaagc aacactcagt attgatatgc aaacttccca
    17161 gtatgctggt agagggcaac atctttgagc ttcctcaatc tctgacagcc acaatatcca
    17221 tcgagcacgt tttgttactc tgtatattct tagaaagttc tttattaaaa attgaaaaaa
    17281 ttgaatcttg gttaaaaacc aatttaacaa aagtgccaaa gaatgatata caataaaaca
    17341 gacgaaaaag tccggatatt gcttttgcaa aatgtgccgg atcctgtaac atcatgaata
    17401 atcgatgaaa cgcttcagga ttgtcggtgt tcaaccacag taaaagcaag acccccagcg
    17461 gcagggcagt aagcgcaatt attgcgggtt tcaataaaaa tcctagtata gatttaaaga
    17521 tactccacca gaggctacta gaataaaaca ctctatattg caattcttgc agttcgccag
    17581 gtgaaatact ccctacttga atgccattga tgtaaatgaa taaatcttta ttagcgttgt
    17641 attgctcctt gtaattatgg ttttcatcta gcacatactc tatacaagta tttccgaata
    17701 ttgaatcgga taattttttt accagcttct tcatttcaaa gtcctttttt atttgaccca
    17761 ttcgaagtgt ttacattcgg gaaacctact acacccccaa taccgcttat cagggttttc
    17821 cttgaatcct ctctgtctca gacggccttc cttacaggtc ggacagacat cgtcttcatt
    17881 tcgagcgacc gcctgttttt ttgtaccagc tgaatccgtc tgttttggcg gacgagcttt
    17941 tcctacagca tcctttttcg cctgcccaac cagctctgtg atcactttct gctgataagc
    18001 cataaattca gctaaagtta atttgccggc ttctatccca gccaaagcca gctcccattt
    18061 ggctgtcgtt atcggatcgg caatcatagt gggaatacgg gcaatcagat tacggccttt
    18121 ttctgtcgca tagattttgc tccgtttctt ttccatataa cccatttcaa acaaacgatc
    18181 gataatggta gcgcgagtgg cttccgttcc caagccttcc gtacttttga gaatttttct
    18241 gatctgttcg tcttctacat tcttcaaaaa gtccttcaaa gtctgcatct catcgagtag
    18301 agaagcctct gtaaagcgcg gtggcggcgt ggtcttttta gtttcgggat tgaaggaaag
    18361 cgtgtcgacc tcatcgttga ctttcagatg tggtagagca ggaccttcat cgatttgatc
    18421 gccttcaaac acgtccttgc ccaaaagaac cttccaaccg gaaataaccg gtgattggct
    18481 ggaggcttta aacaaatgtg aacatgcctc aacctcaact acagtagagt catattcaaa
    18541 atctggataa aactgtgcga tataacggtc tcgtatcatt cggtacaccg ttaattcatt
    18601 cttatccagg actgacaaat caaaattact ggccttggtc ggaataatcg catgatggga
    18661 atgtttgttt acctggttat cgttccatac ccttgattga cgttcaggat cagctttttc
    18721 aatcactgaa agcatggatt tatcgatagt ctgtatcgcg gcaaatacat ctttcacttc
    18781 actcatctgc gattttggca agtatctgca aggggtgcgg ggatatgacg tgatcttgta
    18841 tttttcgtag agggtttgtg caattttgag cactttggaa gccgataggc caagtttttt
    18901 accggcttct ttttgcaaag ccgaaaggct gtacggtaac gggggcgggg tgcttttacg
    18961 ctctttattt gaggtaataa ccttgcctgt agatttctca agctgtttga caagtgcctt
    19021 tacagcctca aggttggtac agtatccgtc agtattacgc cagttttcag gaacctgcca
    19081 cagtgtagta aacactgact tgtctgctgt ctcaaaatct actgaaaggg tgtagtagtt
    19141 gtaaggcacg aaagtatcga tctctttatc cctttggact acaagcttta aagtcggtgt
    19201 ttgaatccgt ccgatcgaca aaatatgttc tcggccatat ccggcagaat aagcagctgt
    19261 gtaggcacga ctcaggttca tacctaccag ccagtcggcg cggccgcgcg ccaaaccggc
    19321 ctgatatagg ttttctttat ccttacccgg cattattgaa gataaggctt tttttaccga
    19381 ttgggtatct aggccactcg tccaaaatcg ggaaatcttt ttacggtagc cacagtagtc
    19441 caatatttca cgggcaataa cttccccttc acggtctgca tcagtagcaa tcactacttc
    19501 atctgctttg gccaacaagc ttttaatgac tttaaactgc ttcgatacct tcggcggtac
    19561 ctgcatctgc caaacttcag ggagaatggg caatgcggag atatttccaa agtcagcata
    19621 ttgttcgcca taagcatctg gcgcggcctg tgtcaggata tgaccaaatg cccaggtaac
    19681 aatagtgtct ccattctgaa aataaccatc atgcctcccg ccaagaacac caagcacttt
    19741 accgatgtcc ttagcttgag atggtttttc acataaaaac aaacgcattt taaaatccca
    19801 agctttgctt cagctgcaaa acaagccaag atgcgatagt caaaaaagcc caaattgcca
    19861 aagcaattaa gccaccataa ataagggtaa caatgaacat atccttaaag tcagcccata
    19921 atgcagcaag ccaacagttc atttcacgaa aaaaggaagt attcttcgtg gccatatatt
    19981 tacaatggga tgtcatcatc agcgtcttca acgtgattat tgtcctgaga ctgctcacgt
    20041 ttaggacgca acttgatagc ttctacacgc tcagttggaa caatgaatac atcttgaaca
    20101 cgcaatttac gaacgcgaac ttcaataccg tcacgattgg tataagactc gtaacgttct
    20161 tcaccggtaa cgaaaaccgg cataccgacc tgtaatattt tatggagatg ggcagcatca
    20221 cgattccagt attcgcaaga aatccactcc gttgaaacca cttctgattt accttcggca
    20281 ttgcgtttga aattaggtga ggcaactgaa aaattcagta cgattgaggc ttcatcgttt
    20341 ttagggcttg ctacaggacg cagattaaat tcagcaccga gattgccacg aacaaaaagt
    20401 tgaactgaca tatttttctc caaaaaaagg atttaaaaat atgacagaaa agacagggta
    20461 aaataaccca atatgagatt aagggttcta ttatcataac taaataaatt cctcttgtca
    20521 actagcaact attacaaatt aaaccttatg ctatatctag ctaaaatgcc cacggacagt
    20581 catcggcaca gaaccgaagt tgacgaaagc cgatgaatcc gccggcggca tccactactg
    20641 taacggggcg ttacagtcac aaaaaaacca tcacaaaagt gatggcaaat gatttttaag
    20701 gctattaaat atcttactta accatgatat tactaggaag atttttgcga tccaaatttt
    20761 caattcttaa aatttcatca aaaatttctt tcatcgaaaa caattctgcc aagtctccgg
    20821 caatctgcct acctcggcaa aaatactgtc tgcaatcaaa ataaaccttc atatgtaata
    20881 accgttcatc aaggctggca ttcgtatcat ccaacgccgt actaatatcg gcccatttaa
    20941 aaaacttctt tctcgacata cggccactgg aagaaacaac atttttgaaa aaaatcatat
    21001 tcggaatata gtattttttt aaatccaaac cagccaaact tttattactc aatttcagat
    21061 gttctcttac tataggatat atgagattaa ggccctgaca tgcttcatga aacgactgag
    21121 cttcatcaat taatagctcg aaatcaacca atttcaattt aatttctttt tctaaatcct
    21181 caatccccct tacccttaaa agccttttgt attcccaaaa ttttaagtat tttttcatac
    21241 ctgatatttg agtattcata gtattcatat cacatcataa aaaaatccat acaaacaagg
    21301 gccaatcaat accatcttaa aactcgattg taaccccacg ttccaatttc acaactagcc
    21361 cgataatcca ttgtcacgaa caatatcaac caacttattg agatcctcct gattcatttt
    21421 tctctggata taaaccaaag agggttgtga ttcatacacc caaacagaca atggaattct
    21481 aggcagaatc tcttgctcaa cagcagattt caacttcgac aactgctctg ggtcaagcag
    21541 actgtcacgt cgaatgctct tgatagccct ggtctccatt accgcaatat ataaatcatt
    21601 cagtagcttt ctcatcatcc gaccgccatt atacaacgtg tcattacatt gtttattagc
    21661 catcatgcct ttgatattaa gtgtcttaac ggcacataca aaactatcgt aatcccgcaa
    21721 caatttaacc agtttgaaac cataagcccg caatacattc aatctgatat gaacagggtt
    21781 attgttctga atcaaggtta tcttcatacc tgcagcggcc aaactatcaa tctgagcatg
    21841 aacctgtttt ttcacagtct caaaaaattt ctcaatttcg gacatctgtt tttcaatctg
    21901 caataaccga ctttgggcat aaggattatc ggacatagcc ataccaaaca actcgttact
    21961 gagtgtcaga ccatatctga ttcccggcca acgtgggccg ttcttcttgt tgttattgcc
    22021 ctcccacata cggatacctt gcttggtatg cataaaaaaa ccatcatcac cggaagaggt
    22081 caggctgcca atatttttaa accggtcgtt ctcagaagaa agaatttcat actcccccca
    22141 aaccaagtga tgctttcgac ctgtctcctt ttgtagatgt tgcttgagca gcaattcatg
    22201 tgctttaaac tcttcccact gttggaactg ttcgtcatcc tcagtcaact tttgattgac
    22261 cttatccccc aaagccaaag cagcacgagc catatcgtaa ccgtcactga aaggtgactg
    22321 ttttgttgtg actttgagat caaattgctg tgcaacatgg atcgcaccag gtttgatgat
    22381 atatctttgt tttgcaaatt ccatttttaa atccttataa gttattaaaa agagcttcat
    22441 aaagcactgt tgttgcatat cagaggctaa ttagtcatgt acaccatcat atctgtcctt
    22501 tgccgatgac tgtaacgccc cgttacatcc gcttcgaagt aatagattta ttcgtctttc
    22561 aaaaattcat caaaaatatt tttttaaatt aaattttatc ttttggagta gaactttctt
    22621 aaattattgc gggcaagctc gacatccatg gcatctaatg agtcctgttt ttggttattc
    22681 tgcttttcaa gataagcctg taatttctga tcctgctcac gctggaatct aacctgtggg
    22741 gcgacaagag cagtaaagtt accttgcgaa gcgcagcggc ataggttaat caaaagacca
    22801 agggggctag taacttcttt tttctgtccc tggatctcat ccaagatttc ctgcctcaaa
    22861 gcccaggaac tttttgagag atgttttgta aaaatcaaat ccgcctgttg acgcatttgc
    22921 acattaaaaa gttttggata aatcaaatca acatcatcaa ccactaccac atgatcttgt
    22981 tcggaagaac gtggtttgtt gggttcaaca gtcatgtagt tgtagttata aacccctttg
    23041 taatttatat aataaataat ctgccgattc ggcaattctg aacttcccca atcgacactt
    23101 atgaatttcc cactacgacc tttcttaaaa ttccaattcg acacatccga atattccctt
    23161 tcgacacttc cggatgtcat acttgggaat aggggggcat caccctcgaa accgctctga
    23221 aaccctttat ccaatccatt tggccctttc cgccaatcac atttggccct ttccgccaaa
    23281 ctgagaattt cgcgtttttt ggagttatat ggactaaagc cgtcgtcctg tttgccagct
    23341 agaagttttt tgatggatat aacaatatcg tgaccattta ccagggtaaa aatacggcgg
    23401 ctggccaaga aatttctctc ataaatgaga tttaaattct tcagcctatt gcgagctgtc
    23461 aattgagatt tataagagag agaggttctt tcctcccaat cagcagcagt caaagtagca
    23521 aaggcatgac gttgtaaatg tggattttga gaaaccaaac caaccgcatc atttaacaca
    23581 cacgacaata atacagccga gttgatacag cctgtaatag aaaccaggtc tcggtgataa
    23641 gcaatccttt tagaaaagtg ttttctaaaa tacagggaac ttgtgttctg taaatcagcc
    23701 agcgtaagtg caagttgatc gtcagtaccg ctcgatttag cgatcaactc agctatggca
    23761 tccaagttca ctttgattgc taaagcagcc cccacacctt tacggcatgt ttgaataagg
    23821 tttgtctcaa gaagcttgtt tttacaagtg cgctgctctc tcactgtcaa accagtttca
    23881 gcttccatct gagcaataga cttgaaaatc caaccatccc gttcggcaac ctctttgctt
    23941 actcttgtcc aatagattaa ctgagaaagc attgcagcag tcttcagaga gcctgtaata
    24001 tcaattaaac atcgatagat ggcaaccctt cgaccacctt gcaatgcttg ccagcattgt
    24061 gtcagttcag agttaaatac tttgggaggg ggtaatttgg tggcggtaac catcacacac
    24121 atccgttcaa atattcagaa gtaagatcag caagcatttt caagctgtat ctacaacgtc
    24181 gcgctgctgg cataccatca acacagctct caatcaaacc aaaagaggtt aattcggcaa
    24241 tagctctttc ctgttgccgt cttgacaaca ttgttgaata aaaaacgtgc ttttcagctg
    24301 catgagtcca aggagaccag tcaccacgct tactcaaatc ttgatattca gcttcacttt
    24361 gaagaaacga caaatacaaa atggatacaa aagcaagacc tctatcggct aatgtcttca
    24421 ggtgaagttt attaatcgaa atagggggcg aaaccatgtc aagtacatct gatttaccat
    24481 gtttacttac caaatcctca aaaagagcat cattcaatgt gaattgtgat tcggttggcg
    24541 ctacggttct ccgattaagc aaaatacctt tctgcagaag gctattgcga atattccgcc
    24601 actgctttgc ggtaatacct gcttcattaa acaacagatc attattcagg cttacccatt
    24661 cgccaggcag acatttctcg acaaaccaac tcaaaaaata tccctctgaa acagaaccga
    24721 ctgcctgagc aaactgacgc acatatgtgg ccggttcctc ccacaaggca accaagctac
    24781 ggataaagtt atcggttgac atgttcttag atggtacaaa accagtctca taatcatagg
    24841 aagtagagaa atccatatac atactcatac tccaaagaac tttacatcgt taattattga
    24901 atgtaattgg gcaaggctat aatcggggaa ttcatcaatc aatagctggt atctaataaa
    24961 caaatctggc tctttttccg acttactcca tgccttcaat accctgacgc gaacatcaat
    25021 agatggcatc acaatgcgcc ctttaacgac aggaatattc atttctatac gcaatttttt
    25081 aatttccgcc gtagaaatta atgaacatag attcataatc atcagattgc tggccttatg
    25141 ttctaacatc catttaagac gctgcatctg ttccataaac ctttgttcgt aatgttccat
    25201 ttctgaaatt ttgcttttta aaaccttcag atcaatggag taggtcacaa catcaggaca
    25261 catggatgct aatttctcaa tccgcttcgg gttcattcgt ttgagcgtgt tcaaaacatc
    25321 ctcatgttca cgagcaacca aagcgttata agaagattca cgcggcgtat ttggtgcgga
    25381 tgccaacctt gtaagtgcaa gattggtata aaccggatcc gtattaaaca gtctataaat
    25441 gggattacta attaaaggct tactcctcac tcttagctcc cgtttttctc aagttgttgt
    25501 actgtgtgct cagagataat atttctacga tattggcgtg aaattcatgg ctggtattag
    25561 tttgccaatt cagtaaagag gcaataatgt tctctttatt accaattccg cagttcgtat
    25621 attcgtcgta cttatcgggg tcctcgtatg caatacgaag cttagaatca cttttaagtg
    25681 tcagcagggg attggaacta tcaccaacat caaacataaa ggtgtcgatt ccactaagct
    25741 tccataagaa tgtgaaaaca tttcccgctt cttcatgcaa aacatcaata gcatagatgg
    25801 catttccggc acttgatgct gaaatatgct caaaagccgg atattcgaca taccagccgt
    25861 atggaaagtc cttatgaatc ttaaagcaat cggacagact cactttggct aacagtttac
    25921 gaacagcaga aaacagtttt ttgctggttt gctccaaagt aatcggttcg gcttctttcg
    25981 gctcatcctc tgcatcagct ttagtaccat ttactttggc tggcacaaat tccatatccg
    26041 aatccttttg atgcaaattg acatcttgtt tggcggctgt ttcaattttg tctttatcag
    26101 aagaattttc tgtttcagaa aaggcgtcag agccatattc aaatttaaat ctgtcgagca
    26161 cgaactgata aagctcatgc acgtctaact ctgttgcaga atattggtta tgccactgct
    26221 ccagtacatc cgaccacaaa tattggaaag aatcgtccaa ctgaattgat ttagaaattg
    26281 agaatagggt agagtgaagt tttttaattt cttctacttt tgcatgggaa agtttgaatg
    26341 cgtattcttg cagctcttgt aaattatcgg aggcaaaaaa gaataatcct aaagtcttat
    26401 gagaaaccgt aaggccttta tcagaaaaca tcacttccag ttcgcgcaaa gaaagtttcc
    26461 ctgagtcaga ttcatcttgg aaaatatcgc ggatttcggc atatttacag gccttatccc
    26521 aaaaacacat ttcagcacgg agttcatttt caacaagatg cgcaacctgc aatgtagttt
    26581 cgtcagagta ggggatgtaa atacaaggca tcatacggaa gcggttatca ccagtttctt
    26641 ccaagagttc gcacaagatt ttgagacggg tattaccacc ctgtgccagt acatatttac
    26701 tttcaccagg tctctgggta acgtgtacag gttgttgaat acctgaagaa cggatggact
    26761 ctttgatggc cgcgtagctg gaaacatcat gtaaacggcg agggtttttg tcaaagaaat
    26821 cgatttcgct gattggcaac gacattaaca tgccccgaga aggagtatcc tcttggccaa
    26881 aactcatatt tgtctgtgca ggggtaccga tgtgcaggct caaggttgct ttattttggt
    26941 ccaaattcat gattgcacct ccttttgcag caggctggcc atgtcattga aacagcttcc
    27001 acgcaggttc ttctgttcga taccagggaa aatctcataa acaatacggt gcatgatttc
    27061 ataagcaggc agcatctttc caagctgttt acgctcatga caatgaaccg ggactcttaa
    27121 agttgtagct tccttatatg ctttggcagc aggaactgct acgcttaaca atttacgtct
    27181 gtcatctaga gtattgctaa agaaatcttt gatagaagat gcgatgaatt tcgcatcatt
    27241 ggtacgatct tgtgcgtaga tgatggctcg taattgaggc atcgggatac gcattgcttc
    27301 gcctttttgc agacgcttta gaagttcttg cgtaccggta aggaactcac gggcactcaa
    27361 aacttcgggc ataattgggc aaagcaggca tgtagcagca aatgctgcgg tatctaagag
    27421 aggaccaact gcaccctgag tatcgattaa aaccacatcg tagttattct gtatgtattg
    27481 ggaatgcagt ttgcttttga gcaagaaggc acggtcggga cgagaggtta cttcagtctc
    27541 gacaaaagca gaaatgttat tggaaataac aatgtctaaa ttcggaaaaa cggtattgga
    27601 aattgtcgag cggatgattt cttcggtatt ttcaccgaga agtaattcaa caataccatt
    27661 tggagcgcgg tggcttattg gatagtattt tgacaatgat ggctgaacat cgcagtcaat
    27721 cattaagaca cgcagtccga tatcagcgag aacagcagag acattagcaa tgagtgttgt
    27781 tttacctacg ccaccttttg tagcggctgc agacaatatt acgggtgcgg acataatatt
    27841 tccttatatt taattgattt ataaagagaa aaacatccgc aatcgaacag gtttaaaata
    27901 tgaaatttac atatttattc tagttcgcat aatgtatatt atgttaaatt tataatggat
    27961 tgaatagata tttttacaat ctcattcaaa actcagacac ttaaaaaatt taatcagaac
    28021 aaataaaaaa gcaaacaaaa atatccatct ttcaaactca tattttgcaa taacctagat
    28081 aagcaagaac aggggcagaa cggattcgcc tttgatgtgg tggtaggcaa ccattccaat
    28141 atgtcccagc gacaaaaaca gcaatgaaaa tgccagcgtg ccgtgaattc ctccgccatt
    28201 gccgatttga cgggcaatgc ctgcggcgga acagcgagca tcagggcata cgcccaatgc
    28261 ccaatttggt gacggcggtg ttggcggggc ggtttttacg ttggcggatt ccctacccta
    28321 cgcaccaaac agacaatatc agcagcagga ttctgactgc acggatatgt tggtatgaat
    28381 cgtccgccca agcggaaaag gctgtcgcgc ccaaaacagt ggcaagaata aattgcttga
    28441 atttcatgat gttgcttctt ttgcacaaag cgttcggaat agtgaagccc gcagcctatg
    28501 gctttgcggc agtcgcagct ttacaagcgg tattgcagga aaccttaaaa cacgatcatt
    28561 gcatcaagag ctgttcgaat tccgccaaat gaacctgaca cgcaaacttt gacgcttgaa
    28621 tacggcattc atagcttcca gtttatcggt tcgataccgt gttgactcaa atagctgttt
    28681 gcctgtgaaa aatggcggca gccaaaaaaa ccgcgatatg ccgacagagg agacggatga
    28741 ggcgcggtca gtatcaaatg attttgactg tctatcagcc tccctttttg ttgtgcatac
    28801 ccaccccaca acatgaagac aaggtgcttg cggtgtgtcg caagctgcct gatgacggta
    28861 tcggtaaagc gttcccagcc taaaagggcg tgcgaatgcg cctgtcctgc acgcaccgtc
    28921 aaaaccgtgt tcagaagcaa tatgccctgc tccgcccacg ctgtcaggca gccgtgcgcg
    28981 ggaatggaaa agccttcgat gtcggtttcc aattctttgt aaatattgag taaagacggc
    29041 ggtatgcgga caccctgccg gacggaaaat gccaaaccgt gcgcctgccc taccccgtga
    29101 tacggatctt gtccgagaat gacgactttg acccggtcga acgctgtcag ccggaatgcg
    29161 ttgaacacat cggcctccgg cggatagata atttgccccg acaaacgttc ctgcctgacc
    29221 gcatttaaaa tttcctgaaa atacggctgc tgtttttcgc cgccgagtgc atcgtgccaa
    29281 gtgtccatat ctgccctatc ctttcaaatt cggctatact gctgatattg cgttattttg
    29341 tcaaacgacg cgctttgtgg ggaaatgtat gactgtatgg tttgttgccg ctgttgccgt
    29401 cttaatcatc gaattattga cgggaacggt ttatcttttg gttgtcagcg cggctttggc
    29461 gggttcgggc attgcctacg ggctgactgg cagcacgcct gccgccgtct tgaccgccgc
    29521 actgctttcc gcgctgggca tttggttcgt acatgccaaa accgccgtgg gaaaagttga
    29581 aacggattca tatcaggatt tggataccgg aaaatatgcc gaaatcctcc gatacacagg
    29641 cggcaaccgt tacgaagttt tttatcgcgg tacgcactgg caggcgcaaa atacggggca
    29701 ggaagtgttt gaaccgggaa cgcgcgccct catcgtccgc aaagaaggta accttcttat
    29761 catcgcaaac ccttaacact cggaggaatt atggaatttt tcattatctt gttggcagcc
    29821 gtcgccgttt tcggcttcaa atcctttgtc gtcatccccc agcaggaagt ccacgttgtc
    29881 gaaaggctcg ggcgtttcca tcgcgccctg acggccggtt tgaatatttt gattcccttt
    29941 atcgaccgcg tcgcctaccg ccattcgctg aaagaaatcc ctttagacgt acccagccag
    30001 gtctgcatca cgcgcgataa tacgcaattg actgttgacg gcatcatcta tttccaagta
    30061 accgatccca aactcgcctc atacggttcg agcaactaca ttatggcaat tacccagctt
    30121 gcccaaacga cgctgcgttc cgttatcggg cgtatggagt tggacaaaac gtttgaagaa
    30181 cgcgacgaaa tcaacagtac cgtcgtctcc gccctcgatg aagccgccgg ggcttggggt
    30241 gtgaaagtcc tccgttacga aatcaaggat ttggttccgc cgcaagaaat ccttcgcgca
    30301 atgcaggcac aaattaccgc cgaacgcgaa aaacgcgccc gtattgccga atccgaaggc
    30361 cgtaaaatcg aacaaatcaa ccttgccagt ggtcagcgtg aagccgaaat ccaacaatcc
    30421 gaaggcgagg ctcaggctgc ggtcaatgcg tccaatgccg agaaaatcgc ccgcatcaac
    30481 cgcgccaaag gcgaagcgga atccctgcgc cttgttgccg aagccaatgc cgaagccatc
    30541 cgtcaaattg ccgccgccct tcaaacccaa ggcggggcgg atgcggtcaa tctgaagatt
    30601 gcggaacaat acgtagccgc gttcaacaat cttgccaaag aaagcaatac gctgattatg
    30661 cccgccaatg ttgccgacat cggcagcctg atttctgccg gcatgaaaat tatcgacagc
    30721 agcaaaaccg ccaaataagc cgtgatgaaa ataccgtctg aagctctgtt ttcgggtttc
    30781 agacggtatt tttatttaaa cccgaacagg gttgtattcc gcttcaaata tgtttcggaa
    30841 ccgtctacct gccccggtaa aaactgccgg caaaatattt ctgcgcgccc tcgcgaagca
    30901 agaccaaggt tacggcggcc aaaggcaatc cggccaacat tccgacaaag cccatcagct
    30961 ctccgaacgc catcagcgaa aagataaccc aaaacggcga caggccgata cggtctccta
    31021 caattttcgg cgtaatgaaa aaactttcga gaaactgacc gacggcaaaa accgcccaaa
    31081 cagccaagat tccgttccac gaaccgaact ggagcaaggc tgcaacagtg gcaagcagca
    31141 atcccgtaaa cgcacccaaa taggggacaa acaccaaaat accggcaacc ataccgatgg
    31201 caaatcccga atccagtccg actagcatca atcccaaacc gtaaaccaag cccataatca
    31261 gcatcaccag aagctgaccg cgcaaaaatt cgcccaatac ctcgttcaaa ttacccgtaa
    31321 tgcgcgtata agcaccggca aaacgcctcg gaaccagttt ggcgatgccg cacgaccacc
    31381 gctgccaatc cagcaggaaa tagtaaagca gcaagggcgg cagcagcagg ttgccgatac
    31441 tgctgacaat attgccgccc tgtttcatca aaacgggaaa ccacgcctta agcgcgttgc
    31501 tcaactcgcc cgtatgcgcc tgaaaccacg caataataga tgcctgatcg atttccacat
    31561 atccgccgat tgtatttttc aaccacggca gcagcgtgtt ctgtataaaa ccgattaatt
    31621 ggggcaggcg agatgccaaa ttattgaact gcccgaccag catagggaca ataatcaaca
    31681 ataatgccaa caacaaaatc aaggaaaaca ccatcacaga catcgaagcg gatgcacggt
    31741 tcaaaccctt tttctgcaac cattcgacca aagggtccaa cacatacgcc agcaccgccg
    31801 caaccgcaaa cggagtcaaa gtatcgccga gcgcgtaaac cagccagacc aaggcggcaa
    31861 acgccgcgcc ggcacccatc cacggcttga tgccccgtcc ttttctccga tacataaaat
    31921 ccacccttcc gaaaataata ttgcgcgcca gtatagcaga accgccgcgc cgtcaaaaaa
    31981 gcctccgcct tcccgatagc ctgttatcgt ggtttaggct aaaatactgc acatccgatg
    32041 ccctctgaaa ggcaaacccg ccttcagacg gcataccgta cacgataagg cagcacatca
    32101 tgaccgaaca aaaaaacaaa gcatccctca tcgaatttcc ctgcgccttc ccattgaaag
    32161 tgatgggcgc ggtgcatccc gagttcgagc aggcggtatt ggacaccgtc cgccttcacg
    32221 cccccgacac gcaggcgcac cacatcacca cgcgtccgag cagcaaaggc aactataccg
    32281 gtgccaccgt tcaggtaaag gttgaaaacc aagaacaatt ggacaacatc taccgcgcgc
    32341 tgacttcgca cgaactggtc aaagtggtac tttgagatga aaatcataca caaaggtttg
    32401 gtcgaatatc tgccgacttt tgaagcgatg aaaaccttta atgccggacg gaatgcagac
    32461 accgaagacg aattgtgggt cgtcgaacac ccgcccgtct ttacgcaagg actggcggga
    32521 aaacccgagc acctgctgat tagggacgac atccccgtcg tccaaatcga ccggggcggg
    32581 cagattacct atcacgggcc cgggcaattg gtcgtttata cgatgattga tttcaaacgg
    32641 cgcaaaacca gcgtccgcaa catcgtttcc gcgcttgaaa acagcatcat cgccacattg
    32701 gcggaatacg gcatcgaagc ggcggcagat cccaaacgcc ccggcattta cgtcggcgaa
    32761 cgcaaaatcg cctcactggg gctgcgtatc aaaaacggtt ccgtctatca cggactcgca
    32821 ctcaacgtca atatggattt aagcccgttt acccaaatca acccctgcgg ctacgccggt
    32881 atggaaatga cgcaaatcgc ggattttgtc caaccctgcc ccgcgccgga cgaagtcgcc
    32941 tccaaactca ccgcacacct tgaaacacaa ctcacaccga aagcagacaa caatgagtga
    33001 aatcaaaacc gacgacccca aacgcggcat caaactcaga ggcgcggaca aaaccgcccg
    33061 catccccatc aaagtcgtcc cccttcagga aaaactgaaa aagcccgaat ggatacgcgc
    33121 caaactccca tcgcgcaaat tctttgaaat caaagacatt ttgcgcgaac aaaagatgca
    33181 caccgtttgc gaggaagcct cctgccccaa catcggcgaa tgcttcagca aaggcacggc
    33241 gaccttcatg attatgggcg acatctgcac ccgccgctgc ccgttctgcg acgtgggaca
    33301 cggccgtccg aatatgctcg accccgacga gccgaaaaac ctcgcagaat ccgtcaaagc
    33361 catgaacctg cgttacgtcg tcatcacctc ggttgaccgc gacgacctgc gcgacggcgg
    33421 cgcccaacac ttcgccgact gcatcaaagc catccgcgaa accagcccga acaccaaaat
    33481 cgaaatcctc gtccccgact tccgaggccg cttggacatc gcactgaaaa tccttgccga
    33541 aaccccgccc gacgtgatga accacaactt ggaaacccat ccgagcctgt acagaaaagc
    33601 ccgtcccggt gccaattatc aacattcttt agacttatta aaacgttata aagaaatgat
    33661 gccgcatatc ccgaccaaat ccggcattat ggtaggcttg ggcgaaacag acgaagacgt
    33721 gcgtgaaatt atgcgcgata tgcgggcgca caatatcgag atgattacca tcggacagta
    33781 cctccagcct tcagacggac acttgcctgt cttgcgctac gttacacccg aacaattcaa
    33841 aatatttgaa aaagaagcat acgaactggg ctttaccaat gccgccatcg gcgcgatggt
    33901 acgttcaagt taccatgccg acgaacaggc ggcggaagcc ttacgcgaaa gccacggcgg
    33961 ttgcgggcat cattaaactc ggaacaccgt tccgaagtaa aaaataattt cctacgctgc
    34021 tgtctgccgc cttgtttgat gtaaacaaca tcaatttgaa ttgtccatgc cgtctgaaag
    34081 tccttcagac ggcataattt attcccaaaa tccgataatc tcaaaagaat ctgtcggtta
    34141 atgagaaaga ttcattataa attttgataa aaaaccgtgc tctatttgtt ttttgctatg
    34201 gttaacaagt gtaaatgttc tttattgatg agatggattc t
//
LOCUS       DAXDSD010000011        32513 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXDSD010000011 DAXDSD010000000
VERSION     DAXDSD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32513
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.011"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..673)
                     /locus_tag="ACFB2Q_000383"
     CDS             complement(<1..673)
                     /locus_tag="ACFB2Q_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917497.1"
                     /translation="MNFVLAKITCKKDLVKILSDDHIFPDFSYENLNFITYNYDYNLD
                     DDTWFQIENLKNQDFCPKFLDNSNLFDSKMFSEIKKEEINIEKLKYLVSCTNDALFFQ
                     KITSSLLLKKKHLLTICGNGAKLCEPQDLLVIKDIPDAVYIIKDDKLIFRTLSSISNI
                     FKGIEDLYREATNTEVQQFLESDFIDLKEDFLSEKVSIPNRKRIALVQDRLNNMTLDQ
                     RQELLN"
     gene            complement(684..1244)
                     /locus_tag="ACFB2Q_000384"
     CDS             complement(684..1244)
                     /locus_tag="ACFB2Q_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917498.1"
                     /translation="MDKLFKILLNISSTSLLLSIYFIKSQYYILDLTWFYRVISNHWI
                     EPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFKQGEVKELEYVNDNFLPSYLGYFF
                     VALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPVFFLFGYRFYQAKTESGLLLVLIS
                     KQEFRTPQSVSSIAVYRINNFTFLEK"
     gene            complement(1237..2439)
                     /locus_tag="ACFB2Q_000385"
     CDS             complement(1237..2439)
                     /locus_tag="ACFB2Q_000385"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC0917499.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDA
                     HIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHF
                     KFLKEFKIILPSKDISQKYNEIANTFFVKVRNNLKQNHHLTQLRDFLLPMLMNGQVSV
                     RCSGARDG"
     gene            complement(2489..2719)
                     /locus_tag="ACFB2Q_000386"
                     /pseudo
     CDS             complement(2489..2719)
                     /locus_tag="ACFB2Q_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(2768..5773)
                     /locus_tag="ACFB2Q_000387"
     CDS             complement(2768..5773)
                     /locus_tag="ACFB2Q_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC0917500.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNANWDRQTNIFPEI
                     FVDSLCRINPDLPPDDARRLLADIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEAFRPDIALLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     PKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSACGKPVFNYFREEHKLNLTEL
                     LDTLSDDLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLAFLLQHGL
                     TYVKASQGSVQKHIMRYPQLFATLAIEKHLSNGGKKGVIWHTQGSGKTAPAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLIVHTIDSREAFAADIKSAQTLHN
                     HAGKPEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDESLGAMVVC
                     DSAEQARQLFEHFQTASDHNFTAALILHDVGTKEERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKK
                     QLLALKKALQTAKELYNILRLQGSHEFLAHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQDDLEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLQTVYTKIKEPNRQNDLLRHKYGGDAKYARIH
                     KRLMENAALYGDKLKVFNALNGVKTDADQRVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQTINRLLVREYLKESGRI"
     gene            complement(5893..7203)
                     /locus_tag="ACFB2Q_000388"
     CDS             complement(5893..7203)
                     /locus_tag="ACFB2Q_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC0917501.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            7400..7701
                     /locus_tag="ACFB2Q_000389"
                     /pseudo
     CDS             7400..7701
                     /locus_tag="ACFB2Q_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714068.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8465..9034
                     /locus_tag="ACFB2Q_000390"
     CDS             8465..9034
                     /locus_tag="ACFB2Q_000390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917502.1"
                     /translation="MSAANNIVEVGGSPMNQEGITAHGNATITLKAKENNKITVENAA
                     YSSDGISTLINRTGARPGTRDDGNKIILEAGGDNIVTMKSGDADADYVNNSKVLTETP
                     YYKSKRGSNGIFAYGDKSLVKLIGENNIVKSEISEKSKALNGGFRHIGIYSWQNAKVE
                     LSAKSDNIVQGGIWGLYSNNSSISLKGKK"
     gene            9152..10756
                     /locus_tag="ACFB2Q_000391"
     CDS             9152..10756
                     /locus_tag="ACFB2Q_000391"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC0917503.1"
                     /translation="MNYKDDTRITGATVSDKGLVAIKPLNNTNIVADTIHYKGDVLAV
                     NKGKVELDFTPNIRLVGRLDNFSGLTDSKHKNLFENYVANLDSKSAGEINFNLAKDAL
                     WTMTGQSWLDKLEGQGTIDFNNDAKTSGRALHIGELAGANKFLMHLNKDGIHSDMLYV
                     KKGTSTPQEVVVKNLSEVLDSMNYGERLRFATVTNSKNEFVNGKKYIDDTHLMEDALT
                     VEYSAHNGDKNNKDDYNKSFNGSEMTAEKAGDDYVNKTYTDNRQNVYLVKQATGNPSR
                     NVKNINDMFDSTAHYAFTLDTYAKREGERAFSTLDKKEGDWIRLTHTRVIQSNAFRFH
                     NNDFEIGYDRFSLNEQEKKRKWGISLDYGHGRTSLWNTFGKDKIRKYELALYNTTQYI
                     DKEGDETGYIDNVLKIGKLRNRVIARNHMGQLWGKGKYSNTLFSISTEYGRRKFLDDD
                     KLWRITPQVQLQYSYLRGTGYRIDNGINVNLSHANSLIGRLGLDVVRKFDGGKKLFYI
                     KGNIFHEFLGSRSFKAFEGKSHYAQK"
     gene            11069..11263
                     /locus_tag="ACFB2Q_000392"
                     /pseudo
     CDS             11069..11263
                     /locus_tag="ACFB2Q_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063514830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(11284..12200)
                     /locus_tag="ACFB2Q_000393"
                     /pseudo
     CDS             complement(11284..12200)
                     /locus_tag="ACFB2Q_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12455..13919
                     /locus_tag="ACFB2Q_000394"
                     /pseudo
     CDS             12455..13919
                     /locus_tag="ACFB2Q_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
     gene            14170..14538
                     /locus_tag="ACFB2Q_000395"
     CDS             14170..14538
                     /locus_tag="ACFB2Q_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917504.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGSIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            14611..16020
                     /gene="thrC"
                     /locus_tag="ACFB2Q_000396"
     CDS             14611..16020
                     /gene="thrC"
                     /locus_tag="ACFB2Q_000396"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC0917505.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            16067..16861
                     /locus_tag="ACFB2Q_000397"
     CDS             16067..16861
                     /locus_tag="ACFB2Q_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917506.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            17049..17825
                     /locus_tag="ACFB2Q_000398"
     CDS             17049..17825
                     /locus_tag="ACFB2Q_000398"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC0917507.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(18483..20954)
                     /locus_tag="ACFB2Q_000399"
     CDS             complement(18483..20954)
                     /locus_tag="ACFB2Q_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC0917508.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCVGCQAVAQSIIDS
                     GLGSYYKRRTADAKKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKDSAAEAVRQLAGKNLTLHILSGDREEAVAETARALGIAHYRA
                     QAMPEDKLEYVEALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKILSEQ"
     gene            complement(20951..22129)
                     /gene="hflX"
                     /locus_tag="ACFB2Q_000400"
     CDS             complement(20951..22129)
                     /gene="hflX"
                     /locus_tag="ACFB2Q_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC0917509.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAHDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            complement(22135..23415)
                     /locus_tag="ACFB2Q_000401"
     CDS             complement(22135..23415)
                     /locus_tag="ACFB2Q_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC0917510.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDALMSAVYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            complement(23567..24292)
                     /locus_tag="ACFB2Q_000402"
     CDS             complement(23567..24292)
                     /locus_tag="ACFB2Q_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC0917511.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            complement(24365..25042)
                     /gene="radC"
                     /locus_tag="ACFB2Q_000403"
     CDS             complement(24365..25042)
                     /gene="radC"
                     /locus_tag="ACFB2Q_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC0917512.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            25069..25191
                     /locus_tag="ACFB2Q_000404"
     CDS             25069..25191
                     /locus_tag="ACFB2Q_000404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917513.1"
                     /translation="MPSETDVPTRQISEMPSEPDFRFRRHLYFKTVLRRFFQLD"
     gene            complement(25165..26514)
                     /gene="gshA"
                     /locus_tag="ACFB2Q_000405"
     CDS             complement(25165..26514)
                     /gene="gshA"
                     /locus_tag="ACFB2Q_000405"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC0917514.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETEKSVLIIPE
                     NHTRNTFYLQNVYALGEIFRSAGCEVRLGSLNPEVTEPAEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGIGQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIDIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGIAD
                     KPFVIVKADAGTYGMGVMSVKSSDEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGVYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            26766..28175
                     /gene="leuC"
                     /locus_tag="ACFB2Q_000406"
     CDS             26766..28175
                     /gene="leuC"
                     /locus_tag="ACFB2Q_000406"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC0917515.1"
                     /translation="MTAQTLYDKLWNSHVVREEGDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGHFTDIRTMA"
     gene            28270..28524
                     /locus_tag="ACFB2Q_000407"
     CDS             28270..28524
                     /locus_tag="ACFB2Q_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917516.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            28586..29227
                     /gene="leuD"
                     /locus_tag="ACFB2Q_000408"
     CDS             28586..29227
                     /gene="leuD"
                     /locus_tag="ACFB2Q_000408"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC0917517.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            29394..30665
                     /locus_tag="ACFB2Q_000409"
     CDS             29394..30665
                     /locus_tag="ACFB2Q_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC0917518.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            30681..31412
                     /locus_tag="ACFB2Q_000410"
     CDS             30681..31412
                     /locus_tag="ACFB2Q_000410"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFC0917519.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNACPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            31446..>32513
                     /gene="leuB"
                     /locus_tag="ACFB2Q_000411"
     CDS             31446..>32513
                     /gene="leuB"
                     /locus_tag="ACFB2Q_000411"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC0917520.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENSVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
BASE COUNT         8179 a         7908 c         8227 g         8199 t
ORIGIN      
        1 aatttaatag ttcttgtctt tgatctagcg tcatattatt caatctatct tgaactaaag
       61 ctattctttt cctattggga atagatactt tttcacttaa aaaatcttct tttaaatcaa
      121 taaaatcgct ttctaagaat tgctgtactt cagtatttgt agcttctcga tacaaatctt
      181 caatcccttt aaatatattt gaaatagaag aaagggttct aaatattaat ttatcatcct
      241 ttattatata aacagcatca ggaatatctt taataaccaa taagtcttgt ggttcgcata
      301 gtttcgcacc atttccgcat atagttagca agtgcttctt tttgagtaat aatgaacttg
      361 tgattttctg gaagaatagt gcatcattag tacaagatac taaatacttt agtttctcta
      421 tatttatttc ttcctttttt atttcactaa acatttttga atcaaataaa tttgagttat
      481 ccaaaaactt aggacaaaaa tcttgattct ttaaattttc aatttgaaac caagtatcat
      541 catcaagatt gtaatcatag ttatatgtta taaaatttaa attctcgtaa gaaaagtcag
      601 ggaaaatatg atcatcagat aatattttta ctaaatcttt cttgcaagta attttagcta
      661 aaacaaaatt cattattgaa ctcctatttt tctaagaaag taaaattatt aattcgatat
      721 acagcaatcg aacttacact ttgtggcgtt ctaaattcct gtttactaat cagcactaat
      781 aataaccctg attcagtttt tgcttgataa aatcgatagc caaataagaa aaaaacaggg
      841 ttaaaataaa atgacttaga gcatgaaact agtaagaaaa taattccata cataacaaat
      901 agcaaaaaaa gattattatc gggtatgctt agagcaacaa aaaaataacc aagatagcta
      961 ggtaagaaat tatcgttaac atattctaat tctttcacct ctccttgctt gaattcatcc
     1021 ttccccaagt atttgcttag ccataagaca gtagcggttg cgataaatgg tattagaaaa
     1081 taaaaaagta gacttaactt agaaataggt tctatccaat gatttgaaat tacacgataa
     1141 aaccaagtaa ggtctaatat ataatattgc gatttaataa aatatattga taacagcaaa
     1201 gatgtactac ttatatttaa taatatttta aatagtttat ccatctcttg ctccgctaca
     1261 tcttacggat acctgcccgt tcatcagcat gggtagtagg aaatcgcgta gttgggtcag
     1321 gtggtggttt tgttttagat tatttcgaac tttaacaaag aaagtatttg ctatttcatt
     1381 atatttttga gaaatatctt tactaggcaa aattatttta aattctttta agaatttaaa
     1441 atgtctagca taaccataac ttgataaatc tatttgattt atgatttggt aaaacaaata
     1501 gtttggcatt cgttcattat tgcttaatat tacttgtgta ccatctgctc cccgcgcata
     1561 ttgaaaatta actaacttaa caattcttgt atgatcacca aaaataatat gagcatcttg
     1621 tgggtttaat atagattttt catcattggt aaatccacaa ataaaatctt gactttggtc
     1681 aacaacagga tattttccaa aatccttaat atctttattt aatatttttg tagtatttgg
     1741 gatttttgct aagcaagatt gtaattctat acttccccac cccttcggaa tttcccgttt
     1801 caaggtttcg tcaaacacca tgtcgccgcc tgaggatttg tagggcttgc cgttggcgtc
     1861 ggggaaatcg aactgcacaa accagtagtc gtacagggtt ttcgccatct cttccaggcg
     1921 cgcgttgatt tgtttgttca gggcgatttt tttgtccaaa gcggatagga cggcggcgat
     1981 ggattgttgg atttgaggtt ctggaatagg caactccagt gtttttaatg cattttcatt
     2041 gacacgttgc ctgccggaag ttccttccat acattcaatc gctcgttttc taaaatcggg
     2101 actgattgca aaataataga gaaattcggg attggtttca ttttttgctc ttaacacgat
     2161 aaattcggta gaaccgaaag ccacttctcc atcatctaaa atatctacaa aagcggtttt
     2221 cccattttcc aaacacgggg taatcttagc taataaggta tcaccattgc gaaacttagc
     2281 tccaccgtta aatgccttga tttcgtaacc cgtaatttgt cgttgaaatt ctttcaacat
     2341 tgccatcgga acactttttg ccaatgctcc cttggctagt tgctctctcg gattaaaatc
     2401 agcaatattt tgaatttgtt gtttcttaaa atgattcatg atgttccgtt acaaaactct
     2461 tcaaaggccg tctgaaaaaa acatttcatc ggtagttctt gccgtctgcg gcagttgtaa
     2521 actgaaagtt tacaactgaa tcgtccagtt cttgttctgc aaatatggct ttgatgtgtt
     2581 taccgatgtt ttgtttgctg gtttggtaga gttcggcaat ttctttttgg gcaagccata
     2641 gttcgccgtc aagctcgcgc agggtaaatt cggatagtcc gtcttccgtg gtgtagatga
     2701 tgatgtcgtt gttgtgcata gctttcctgt atctataaat ggttaaggct acctgaaaat
     2761 ctacttctca aatccgcccg ctttctttta aatattcccg taccaacagg cggttgatgg
     2821 tctggatgtc tgcgggttga acaggaaatt gctgttccgt tctaaagcgt tttaatacga
     2881 tgccctgcat ttgtttttca aagtaattct ggttgtccaa aatctgctcc atatccagca
     2941 ctctttggtc ggcgtcggtt ttgacgccgt ttagcgcgtt gaagactttg agtttgtctc
     3001 cgtagagggc ggcgttttcc atcaaccgct tgtggatacg tgcgtatttg gcgtcgccgc
     3061 cgtatttgtg ccgcaagagg tcgttttgtc ggttcggctc tttgattttg gtatagacgg
     3121 tttggagtgt ggcaatgttt gcctgcattt cttcctgccc gacttcggcg aggttctttt
     3181 tcttgaagat gcgttccagc tcttcgcgca ggctgatgta ttcaaggtcg tcttggtcga
     3241 agttgcccgc cagcccttcg cgcactttgc gcatgatgtc ttttaaatcg tcggcaagtt
     3301 tgagttccgc ctcgccgatt ttgacgaatt gaaagtaaac gtcttccagc gcttcattga
     3361 gcaaatgggc ggtgtcgcct tgctgtaatt tttcggcaag gtttaaggta tccaagcggg
     3421 cggcggtttc gcggtacaaa agattgagct tgtcaaaatc cagatgcgca agaaattcgt
     3481 ggctgccttg caggcgcagg atgttgtaca actctttggc ggtttgcagg gcttttttta
     3541 aggccagaag ctgttttttg tcttctattt ggctaatttg gctgcaaaat tcttcggcgt
     3601 tttcggtatc gaaatcaaac agggcgtttt taatgtctgc aatttcctgt tcgatttctt
     3661 cggcggtttt gaatagctgg ctgtaactgc cgatttcgtc gcccaattcg ttggaaagtt
     3721 cgtcccaata ggcgcggttg gttttgtcaa attcgcgttc gatatcggca aaatcgacga
     3781 catagccgta gcggtaagat ttgtaggtgc ggttgacgcg ggtcagggtt tgtaaaaggt
     3841 tatgggcttt tatcagtctg cccaaataga gctttttcag gcgcggcgcg tcaaagccgg
     3901 tcaaaagcat gttgtacaca aacaagatgt cggttttgcc cgctttgaaa tctttaaccc
     3961 attggtcgcg ttcttcctta gtgccgacgt cgtgcaatat caacgcggcg gtgaaattgt
     4021 ggtctgaggc cgtctgaaaa tgctcaaaaa gctggcgtgc ctgttctgcg ctgtcgcaaa
     4081 cgaccatggc accgaggctc tcgtcttggt tggtttttct gaatttggcg aagtcgtcca
     4141 agatgtagtc aagcatcggg tggacgaagt gcggatgggc gtagatttct ttgcggtcaa
     4201 agctgccttt ttcgatttca agttgcgcca gtgcttcttg taattgcgcc ttgtatcggc
     4261 tgccgatttc ttcgcgtatc aggcgcaggg tgtagccgtc ggcaatggag gcgttgtaat
     4321 agtatttgtg gatgtagtcg ccgaagagtt cgcgggtgtt gacgttgcct gcggttacgc
     4381 cgataagcgg cgtgccggtc agcccgattt tgacggcgtt tacgtcggac tgattaaggt
     4441 tggcaagaaa tgagcctttg gggttgtagc tgcggtgtac ttcgtcgaga aaatagacgc
     4501 gctgaatggc gaggtcgtag tcgttacggg cgacgacatc ggggtcgtct tggaattttt
     4561 ggatgttgac gacggtgatt tccggtttgc ctgcgtggtt gtgcagggtt tgggcggatt
     4621 tgatgtcggc agcgaaggct tcgcggctgt cgatggtatg gacgattaag tcgcgggcgg
     4681 taaattcccg ctgcgcctgt ttcaataaat cgagcctgtc cacgatgaaa tagaatttcg
     4741 gcacgatacc ctgtttggcg taatagtggg tcaggtaacg ggtgttgtaa taggcaggcg
     4801 cggttttccc cgatccttgg gtatgccaaa tcacgccttt tttaccgccg ttcgataaat
     4861 gtttttcaat ggcaagggtg gcaaacagtt gcggataacg catgatgtgt ttttgcaccg
     4921 aaccttgact tgctttgacg taagtcagcc cgtgttgcag gagaaaggca agacgttcgc
     4981 ggcaaagcag cgaagtcagg atgcggttgg tcggcgtatc gggcgattta ttgctgataa
     5041 attcggggct gtgtttgata acgggcagat tattgtcttg tagaacgttg ttttcaaggt
     5101 cgtctgaaag cgtgtccaat aattcggtaa ggtttaattt atgctcttcg cggaagtagt
     5161 tgaatacggg cttgccgcaa gcagacgagg cgtaaaacgc gccttgtgcc ggctcggttg
     5221 cgccgtcgtc gtattccatg ttgttggaaa aaatcatgaa ttgggtgatg ttgataaaac
     5281 ggcggaattt gggatttttc gcacgtttgc ccatgcgctc ccgctcttcg ccgatgccgc
     5341 ctttattgtt gggctttttg acttcgataa agaccaaagg catgccgttt accagcaggg
     5401 caatgtcggg gcggaatgct tcgtcgccgt tgatacaagg caactcagtt acaacgtgga
     5461 aactgttgtt gtcaaaattt tggaaatcga tcagcttctt accgccggat tgattggtga
     5521 ggcgttcgta aaatttttgc cccaaatctt cgttatccag ctctaagcgt atgtcggcaa
     5581 gcaggcggcg tgcatcatcc ggcggcaaat cgggattgat gcggcagagg ctgtctacaa
     5641 agatttcggg aaagatattg gtttgtctgt cccaatttgc attttttagc gaaagataat
     5701 catagcccaa gcgcatcagg tgcaaaacgg cggggatttt gacgcgggaa ttttcgttgt
     5761 gggtttggga cataaacgta acgtaatggg tcatcaaaat ttattgaaac ggattataac
     5821 acctgaaaaa agccgatacg ccttttcggt gtaccggctt tgccatactg ttctgcttca
     5881 gacgacatcg cttcattttg cctttaatgc ctcttcgtcc agcgatttca accatgccag
     5941 cttttcgccg attttgattt ccagcccgcg cgggacgggt tggtaaaagt ccggttcgtc
     6001 caagccgtcg ggcatataac tttcgccggc ggcgtaggcg ttcggttcgt cgtgggcgta
     6061 gcggtattcg cgtccgtagc ccaattcctt catcagcttg gtcggggcgt tgcgcaggtg
     6121 gacgggcact tcgtcgctgg cgttttcttt gacgaagcgg cgcatttggt tgtatgcctt
     6181 gtagcccgcg ttggatttgg cggcggcggc aaggtacagc acggcttgcg ccagcgcgag
     6241 ttcgccttcg ggcgagccta agcgttcaaa ggtggcggcg gcatcgttgg cgatttggaa
     6301 ggcgcgcggg tcggcaagcc cgatgtcttc ccaagcgata cgcacgatgc ggcgggcgag
     6361 atagcgcggg tcggtgccgc cgtcgagcat acggcaaaac caatacagcg cggcgttcgg
     6421 atgcgaaccg cgcacggatt tgtgcagggc ggagatttgg ttgtagaaac tctcgccgcc
     6481 tttgtcgaaa cggcggattt gcgcgcccaa gctgtcggcg agaaattcgg tggtcaggat
     6541 tttcagacgg cgcgtgtcgg cggcacgtaa aagttgttcc aacaaattca ataatctgcg
     6601 cgcatcaccg tcggcggtat tgacgagcag ctcttgcaca tcggcttcaa tcgtgaaatc
     6661 ccggtattcg ggcaaagcca acactttggc aatcagtttc ttgaggtcgt ctgaagacaa
     6721 ggattgcaaa acatatacct gcgcgcggct cagcagcgcg ggattgactt caaacgacgg
     6781 attttccgtc gttgcgccga taaaggtcag caaaccgctt tcgacatgcg gcaaaaacgc
     6841 gtcctgctgc gccttgttga agcggtggac ttcatcgaca aacaaaatcg tcgcgcgtcc
     6901 ctgctgcaaa gcgatttcag ccttatcgat tgccccgcgg atgtccttca cgccggaaaa
     6961 cacggcggaa acaggcaaaa actgggcatt gaaactctgc gccaaaatcc gcgccaacgt
     7021 tgtcttgccc acgcccggcg gcccccacag caacatagaa tgcggcttcc cgccttccac
     7081 cgccacgcgc agcggcttgc cttcgccgat taaatgctgc tgcccgataa catcatcaag
     7141 cgtatgcggg cgcagacgtt cggcaagcgg tgcgtcgggt tctcggacaa acaaatcggt
     7201 cataacggct ccgtcaacag gttttcagac aatatgatta tacggcaggg aaaggcggcg
     7261 tgccgcatac ggataccgcc ccaccgtttg tactaagccg atcttaggcg tacaattggg
     7321 aaatttcagg aaatttatac gctatgccat ccatgcagct ttgcaaccgg acccgcaaag
     7381 accaggtccc gttttaacca tgcggggaag ttaagattac agtaaacaaa actattttgc
     7441 cttattggaa gaaaggggaa cagttatgag aaattggaac ttattaccac cggggaaggg
     7501 gcagatggag acttactggt caacggttct tacggcatcc gttcttttgc agacctaaaa
     7561 ttggaataca aacccgaatt atcggtgaga agcaatcatg acattatttt caacaatgat
     7621 tttcaatctg ttgacgtaca accctattcc ggaaatatcc atttggatgc caaaggaaaa
     7681 attcaattta atttgaatgt tgccgataag gtaacagatc caaccacaca tcaagaaaag
     7741 ttagtgaacg ataacgcttt agtgcggtat gtcaacaata tcaccccctc cgatgaaaat
     7801 gacaaaggca gcatatcctt ccattcaaaa acaggcaatt tgttcaatat gaacgttaac
     7861 gttcataaag gcacacacca tttcggtatt cacaacctcc gcaataccaa tgttaatctg
     7921 actgccgaag acggcgataa tgtggttgcg attaaaggga agcttgccga gcctatgccg
     7981 gcaggaaaag ttgagagtac aagtttttta aggggtattg aaacggcttt taaatccaat
     8041 accgtattaa cggggaataa caataaaata tccatcacca ctgagggcga cggcttggga
     8101 ttgggcgtaa atggcctcca gtccaactcc ccctattccg gacacacgac gggtactttc
     8161 atcaataatc aaccaagcaa tactacccaa ccttcccttg acggcaccag caccatcgaa
     8221 ctcaaagccg tcaaggggaa taatgaagtt gatttaaatg taaaaaacca cgcctccgta
     8281 aaaggcatta tcacgtcgca ttctgcaaaa gcaacgcttg aagccgagga ggataatatt
     8341 gtccgggtta aaaatccgga caccaagacg gctttaatca atgcctggaa agaaaaatac
     8401 cccaataatt cgccagaacc atacagggtg gggggttcaa tccacgattg acagcgatgt
     8461 agttttgagt gcggcaaaca atatcgttga agtaggaggc agcccgatga accaagaagg
     8521 gattaccgct cacggaaatg ccacgattac cctcaaggcg aaagaaaaca ataaaattac
     8581 cgtggaaaac gccgcataca gcagcgacgg catttcgact ctgattaaca gaacgggggc
     8641 aagacccgga acaagggatg atggaaataa aatcatactg gaagccggcg gcgataatat
     8701 tgttaccatg aaatccggcg atgcggatgc ggattatgta aacaattcca aagtattaac
     8761 ggagacacca tattataaaa gcaaacgagg ttccaacggc atttttgcct atggcgacaa
     8821 atcgctggtc aaactgattg gcgagaataa tatcgttaag agtgaaatca gtgaaaaatc
     8881 taaggcatta aatgggggat ttcgccatat cggcatttat tcatggcaaa acgcgaaagt
     8941 cgaattgtct gcgaagagcg acaatatcgt acaaggcgga atttggggct tatactccaa
     9001 caactcctca atttccctca aggggaaaaa ataatgtgat ttcaaacccg aaatataatg
     9061 ttttcgccta caaaaaggca aaggtggatt tggtcaaatt accattgact ccaacgttgc
     9121 caatcttgca aggcaagatg gttcaattca tttgaattat aaagacgata cccgtatcac
     9181 aggggcaacc gtatctgata agggtttggt agccatcaaa cctttgaata acacgaatat
     9241 tgttgccgac actattcact ataaaggcga tgtcttggcg gtaaataagg gtaaagtgga
     9301 attagatttc acgccgaaca tccgtttagt gggacgtttg gataatttta gcggcttaac
     9361 cgattccaaa cataaaaatt tattcgaaaa ctatgttgca aatttagaca gcaaaagtgc
     9421 gggcgaaatt aactttaatt tagccaaaga cgcattatgg acgatgacag gtcaaagctg
     9481 gctggataaa ttggaaggac aaggcactat cgattttaat aatgatgcta aaacaagtgg
     9541 acgcgcctta catatcggtg aattggcggg tgccaataaa ttcttgatgc atctgaataa
     9601 agacggcatt cacagcgata tgctctatgt gaaaaaaggc acttcgacac cgcaagaagt
     9661 cgtcgtcaaa aatctgtccg aagtgctcga cagtatgaat tacggcgaac gtttgcgttt
     9721 cgctacagta acaaactcaa aaaatgaatt tgtgaacggt aaaaaatata ttgacgatac
     9781 gcaccttatg gaggatgccc tgactgtcga atactccgca cataacggcg ataaaaacaa
     9841 caaggatgac tataataaat cctttaacgg ctctgaaatg acggcggaaa aagctggaga
     9901 cgattatgtc aataaaacct ataccgacaa caggcaaaat gtctatttgg tcaaacaggc
     9961 taccggcaat ccgagccgaa atgtcaaaaa tatcaatgat atgttcgatt caaccgcaca
    10021 ttatgcgttc actttggata cttatgccaa acgcgaaggg gagcgggctt tttcaacgtt
    10081 ggataaaaaa gaaggcgatt ggataaggct gacgcatacc cgtgtgattc aatccaatgc
    10141 gtttaggttt cataacaacg attttgaaat cggatatgac cgattcagcc tcaacgagca
    10201 ggagaaaaaa cgcaaatggg gcataagtct cgactacggc cacggcagga catcattatg
    10261 gaatacgttt ggcaaggaca aaatcaggaa atatgaattg gctctgtaca atactaccca
    10321 atacatagat aaagaaggag acgaaacagg gtatatcgac aatgtattaa aaataggaaa
    10381 actgcgtaac cgtgtgattg cacgaaatca tatggggcaa ttatggggca agggaaaata
    10441 tagcaacacc ctattctcta tcagcaccga atacggccgc cgtaaatttt tggatgacga
    10501 taaattgtgg cggattacac cgcaagtaca gttgcaatat tcctatttga gaggtaccgg
    10561 ctatcggatc gataacggca taaacgtcaa tttaagccac gcaaacagcc tgataggccg
    10621 cttgggtttg gatgtcgtga gaaaatttga cggaggcaaa aaacttttct atatcaaagg
    10681 caatatcttt catgaatttt tgggcagtcg ttcctttaag gcatttgagg gcaaaagtca
    10741 ttatgctcaa aaatgaaata cgagggggac ttggtattcc gccggattgg gctacaatgc
    10801 gcggacaggc aaaaaaaacg aatgtctttg ccgatgcgga aaaaagagtt ttccggcggg
    10861 aaaaaaggat cgtacaacat ccgcttatcc gtttcccatc agtttgactg aatgatatcg
    10921 gggccgcgcc attattcggc aggccccatt ttattgccac acattgcctg cccgattccc
    10981 gtttcaacag caaacccttc caatattttg ttcaaacttg gatcgcggca gatgggggcg
    11041 tgcaagcccc gcaaggtttt tttggctttt acggaacagc acgcatttcg ccaaacggca
    11101 aaattgcatt aacggaattg aaaacttttg gaattgtgca aaacggcatt tgcgtcagtt
    11161 taacggcatt tccaaagggc attttaagcc gtattcgaga gaatgcggat ggcgttttca
    11221 gtgttttcag cgtgataaaa ttcaaatttc tattctaaaa caatcagcaa agcaggattt
    11281 atttcagttg tccgagacaa cgggcggctg caccgcctgc actccccata ctttttcaac
    11341 gcactcgagc aggagtgaga cgtggggatt atcctcgtat tcttccgcat aaatgaaatt
    11401 gagcggcata ctgtggcggc acgattcgat aatagccacg cccgcgcctt cttttgccgc
    11461 cgcttcggct ttgtttccct gcaccattgc cacacctata ccgcctgcaa ccaaatcgat
    11521 aagggtttgg gggtagtcgc acaatatctg tttttttggg tgagagccgg ttgctgcgcc
    11581 aaaactggtg caggtgcttc ctacttccgg acacgcccga catttctatc catacgcatt
    11641 cttgcaggct ctgcggaagg gagcgggtca gccgggaata ttggctttgc gggcaaatca
    11701 gcgcgtaggt caggttttgc aggaatacgc tgcggatgcc gccttgggcg gcgttgccga
    11761 gtataaagcc gccgtgcagg gttttgtgtt ggatgcgcga gaggatttcg ccgctcattc
    11821 cgtattggat gtgcaggcgc gttttggggg gctgtttgac cgatattgtc cgtcagcgcg
    11881 acgagttttg ccgaatcgat ggggtggata atgcccaaac tggtctcttc cgaataatcg
    11941 cctgccagcg ttttggcaaa atactccaac ttgtgtttgt attgcagcag ggattccgct
    12001 tcgggcaaca gtatttcgcc cgcccgcgtc aataccatgc ctttccccgt gctcctgaac
    12061 agcggcgtgc cgacatattc ttcaagggct ttaatttggg cggaaacggc gggctgggaa
    12121 aggaaaagtc gtttggcggt ttgggtaagg ttgccctcgt gcgcgacggc gacaaatgat
    12181 tttaattgta cggcatccat gtatccctcc ttgtgcggat gttttctata tttgtgcaat
    12241 cgaaatcttt taggcggatt gttgctgaaa attaactttt taatcaagtg gtttgtaaat
    12301 tgtatcagtt ttccggatga tgattattaa aaaaaaattg gttttattgc ctgtttgggc
    12361 tttaaatggg gttacggctt ccgaacgcag cccgtatcaa aaagaaaagt catgcgcccc
    12421 cttttacgag gcgcgatata taaggaggaa ggttatggaa aaacataatg ggacttatcg
    12481 ggatttacac cgtccggctt cggaatttgc gacgcgggac gaatatttgg aacatgaatt
    12541 gcagattatg caaccaaaac gctggcggcc caacctgccc tttcgcgatt accgcttcga
    12601 gtgggaggat ttgattcctg cgatggcggg aacgattgga aaagtggtga tggtgggggg
    12661 gcggcggcgt ttgccgcacc tttggggctg cctgacagct ttgtactgga aaatgtgcgc
    12721 tatgagcttt taatcgccgc cgcgtttatc ttattggtat cgggcttttt tctgcccggc
    12781 gccaacctgc ccggtacgca cgggccgctg attccgatga ttcccatcgt tgtgtcggca
    12841 ggcgggcatc ctttggcgtt cggcatttcg attgcggttt taggtctgct gatggcttta
    12901 tttcgcggcg gcagtattat ggcgaagctg acaagcaacg gcgtatgcgg cggattatta
    12961 ctctatttgg gctttatcgg cacgacgggg caggtaaaaa aattgttttc gtgggcaggc
    13021 ggttttaata tgccctacat cgcttttacc gtcattattg taacgattgt gatgtacgct
    13081 ttgttggagc attggaaaaa acgctggttg gccgtgcctt tgggatgctt gattgccggt
    13141 gtggcggcat ttgcattggg tgcgccgttt gagtttcaca ccgcccccgg cctgcctcca
    13201 atgagtcctg cttattggta gggtgaaaac agcggctggc atctggggtt gccgacggca
    13261 gaaagttttt tggttgtctt tccatttgcg gtattggctg ttgcaatgtg gtcgcccgat
    13321 tttttaggac atcaagtgtt ccaaaaaatc agctatccgg aaaaaaccga taaggtattg
    13381 atgaatatag acgacaccat gacaagttgt tctgtccgtc aagcagtggg ttctatttta
    13441 gggggtgcaa attttacctc ttcttgggga acttatatcg tgccggcatc gattgccaaa
    13501 cgccccattc cgggcggtgc ggttttaacg gcggttttat gtattatcgc cgggttgtgg
    13561 ggctatccga tggacttggc gatttggcag ccggtattga gcgtagcctt gatcgtaggc
    13621 gtatacttac cgcttttgga agcgggcatg gaaatgacgc gcaaaggcaa aaccacccaa
    13681 tccgccgcca tcgtggtgtt ctcttccgtc tggtcaatcc ggttttcggc tgggcgttga
    13741 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga acgcagcgcg caattaggtt
    13801 ttgtcggacg agtattgata cccgcagtag gtttcttaat cttgtgtgtg gcgatgggtg
    13861 cggtcgggat gctgcccggt atccctccgt ttttggagca gttcaaatct ttgggctagg
    13921 ctgaaatcgc aaatgccgtc tgaaccggtt tcagacggca tttttgcaaa caggcaaaat
    13981 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
    14041 atattcaaac ggtttgctcc aacactcgga acggcgcata aaacgccgcc cttcgcgtta
    14101 tcccgaacgg ggcggctaat cagatcctat cgccataaaa ggcggggttt caaccgaaaa
    14161 ggaattgaga tgaataaaac cttgtctatt ttgccggcgg caatcttact cggcgggtgc
    14221 gccgccggcg gcaacacatt cggcagctta gacggcggca cgggtatggg tggcagcatc
    14281 gtcaaaatga cggtagaaag ccaatgccgt gcggaattgg acaggcgcag cgaatggcgt
    14341 ttgaccgcgc tggcgatgag tgccgaaaaa caggcggaat gggaaaacaa gatttgcggc
    14401 tgcgctaccg aagaagcacc taaccagctg accggcaacg atgtgatgca gatgctgaac
    14461 cagtccacgc gcaatcaggc acttgccgcc ctgaccgtca aaacggtttc cgcctgcttc
    14521 aaacgcctgt accgctaacc ttcttcagac ggcatgaggc agatgccgtc tgaatccgtt
    14581 ttcctgcaat ttcgacaaac gagaaaaatc atgagataca tcagcacgcg cggcgaaacc
    14641 gcacacaaac cgttcagcga agttttattg atggggcttg cacccgacgg cggactgatg
    14701 ctgccggaac attatccgca aatcgggcgc gaaaccttgg acaaatggcg cggtttggct
    14761 tatcccgaat tggcgtttga aatcatgtgc ctgttcgtta ccgatattcc ggaggacgat
    14821 ttgcgcgaca ttctgaaccg tacttacacg gaagcggcgt tcggtactaa ggaaatcacc
    14881 cccgtccgca cgctttcaga cggcatcaaa atccaagcct tgtccaacgg cccgacgctg
    14941 gcgttcaaag atatggcgat gcagtttttg ggcaatgcgt ttgaatatgt tttaaacaaa
    15001 gaaggcaaaa aactcaatat cttgggcgca accagcggcg atacgggttc ggctgcggaa
    15061 tatgccttgc gcggcaaaaa aggcgtgaac gtatttatgt tgtcgcccga cggtaaaatg
    15121 agcgcgttcc aacgcgcgca gatgtacagc ctgcaagacg ggaatatcca caatatcgcc
    15181 gtgaagggga tgtttgacga ctgccaggac attgtgaagg cagtgcagaa cgatgccgcg
    15241 ttcaaggaaa aataccatat cggtacggtc aattcgatca actgggggcg catcgtcgcg
    15301 caagtggttt attactttgc aggctatttc aatgccactt caagcaatga cgaaaccgtc
    15361 agcttttgcg taccgagcgg caacttcggc aacgtttgcg cgggacacat cgccaaacaa
    15421 atgggcctgc ctgtccgctg cctgattgtc gcgaccaatg aaaacgatgt gctggacgag
    15481 tttttcaaaa ccggcgcata ccgcccgcgc aacagcgcgc atacttatgt aacctccagc
    15541 ccgtctatgg acatttccaa agcgtccaac ttcgagcgtt tcgtgttcga cctgatggat
    15601 cgcgatcctc aggaaatcaa tacgctgtgg gcggaagtcg cggcaggcaa aggctttgac
    15661 ctgcggtttg ccttggacaa agtcggcggc aaatacggct ttacctccgg caaatctacc
    15721 cacgccgacc gcctcgccac catcagacag gtttacgagc aagacaaaga actcatcgac
    15781 ccgcatactg ccaacggcgt aaaagtcgcc cgcgaagtgc gcgaagcggg ggaaacggtg
    15841 gtttgtttgg aaaccgcgtt ggcggcgaaa ttcgatgcga ccatacgcga agccgtcggc
    15901 gatgtcgcca ttccgcgccc cgccgcgctg gaaggtttgg aaaacctgcc gcagcgcgtg
    15961 caaaccgtgc cgaacagtgc ggatgcggta aaaggcatca tcgaacaaac ccttgcctga
    16021 tgttccgtat gggcggcatt ccatccgaac ttaagattga aagaaaatga ccttacctat
    16081 gcaggaaacc cgtttttcta tactgctgga cgaattggcg gcaaaacagg aggcaaccat
    16141 cgccccctac ctgcttgccg acgggaccaa ggtatgggta cgcaaagcag gcaggcacaa
    16201 tgcgcgatgg cgttacgcgc tgctcggtat ggttgcccga tatttgaaac tgggggtgtt
    16261 gaagccggtt cccagcctcg gcggcgagcc tgccattgca accgaatcaa aacgcctgta
    16321 cgaattgcgt tcggcaggga tagccgttcc cgaattgctc gccgtccgga aaaatgccct
    16381 tatgttcggc aatttggaag gcatcccgct cgatacgcaa atccgccaag aagccgaagc
    16441 cggaaaggcg gacgcttggc ttgccggttt ggaagccatt gcacgcgtgc ataaaaaacg
    16501 gcagttcctc agtcaggcgt ttgcgcggaa tatgatgtgg gacggaaaaa acatcagctt
    16561 tttggatttt gaagacgacc cttccgaggt cctgaccata gcgcaatgcc aagcccgcga
    16621 ttggctctgc tacatccatt cgaccgcgct gatattgaaa aacggcggac tgctggaagc
    16681 ggctgcggaa aaatggggcg gcgtattgtc ggatcagcct gccgaaatac aaaagctgat
    16741 tgccggcacc gtcaaaccga tcctcccgat acgcaggctg gaacaccccc gctggggacg
    16801 ggacgcgctc aggctggcag cctcgatttc cttaatttcc ctcgccgata tgccgccgta
    16861 agccaccgcc cgccccacga tgccgtccga agcccttcag acggcatata accaacgcgc
    16921 atcaggcatt gtcgcaacca tccgtttcgt ttagaatttt gactttatcc cgaatcgggc
    16981 agacatttcc actctgccgc acgtcccgaa caagcactat ccatttcttt tactgacagg
    17041 aaacccaaat ggcagcattc aatacccaaa aagtattgtc cgtacaccac tggacagacg
    17101 catattttac ctttacctgc atccgcgacg aatcgttgcg ctttgaaaac ggacagttcg
    17161 tcatggtcgg gctgatggcg gacggcaagc cgctgatgcg cgcatacagc gtcgcctccg
    17221 ccaactggga agaacacctc gaatttttca gcattaaagt ccaagacggc ccgctgacca
    17281 gccgcctgca acacctcaaa gtcggcgacg aagtgttaat cagcaaaaaa ccgaccggaa
    17341 ctctagttgc ctgcgatctg aatccgggca aacaccttta cctgttgagt accggtaccg
    17401 gcatcgcccc tttcttgagc atcaccaaag accccgaaat ttacgaacag tttgaaaaaa
    17461 tcatccttgt acacggcgtg cgctacaaaa aggatttggc atactacgac cgctttacca
    17521 aagaattgcc cgaacacgaa tacctcggcg acttggttaa agaaaaactg atttactacc
    17581 cgattgtttc ccgcgaagaa ttcgaacacc gcggccgcct gaccgacctg atggtaagcg
    17641 gcaaactgtt tgaagacatc ggcctgccca aaatcaaccc ccaagacgac cgtgccatgc
    17701 tgtgcggcag ccctgcgatg ctgaaagaca cctgcaaagt tttggacgat ttcggtctga
    17761 cagtctcccc gaaaaccggc gtgcgcggcg actacctgat tgagcgcgcg tttgtggatc
    17821 aataagccgc ttgaaaatgc cgtctgaacg gcatcagcga taaaaaaacc cgaatcctgc
    17881 caatcaggat tcggggtttt tatagtgggt aaaaattaga aatgcacatt tccgtcattc
    17941 caacacaggt gggaatccgg acttacctgt ttcattatcg tttgaaaata aaagagaaac
    18001 caaacatata gattcccgct tgcgtgggaa tgacgacggt gttttgccgt agccaaagca
    18061 ggaaaacatc cttattgtca tttcatcatg ataggacagt aaggatgtta aaaattaatt
    18121 atcattatat gattgatata taataaaaat tatcccaata agaaatgtta tgcagaaaat
    18181 aaaatgaaaa ggatggtttt cattgaaaat ggttgttctg ccaccgtcat acacagaacc
    18241 ggtatttgga aaaaaaataa tccatattga catcattatt ccatttatcg cataaatcca
    18301 ctttttaatt tttttcctca ttaatataat ctcttattta ataaaatcaa atacctagca
    18361 attcgatctc aaattttaaa cgccaacgtg tttgagcggt aacaatgcat actttaagtt
    18421 tgtaatcctt catcgtctca tttttgtttt aatcgactat acgtctaaag caacgccgcc
    18481 cgtcattgtt cggacagtat tttttcagac ggcattttcc cccgtttgtg aaggcgcaag
    18541 gcattgccca aaaccgccag cgaactgaaa ctcataccca gtgcggctat ccacggttgg
    18601 acatagccca aaacggcaag cggtacggcg atgatgttgt acgcgcccgc ccatatcagg
    18661 ttttgccgga taatatggcg ggtgcgccgc gcctgatcga gcaggtgggc gacggtacgc
    18721 aaatcttcgt tcaataacac aatgtccgcg ccgtccctcg caatatccgt cccgcccgct
    18781 gcggcggcgg atacgtctgc ctgcgccaaa acgggcgcgt cgttgatgcc gtcgcctatc
    18841 atcagcactt ttttcccttc tttttgcaag gcttcgacgt attccagctt gtcttccggc
    18901 attgcttggg cgcggtagtg cgcgataccc aaggcacggg cggtttcagc aactgcttct
    18961 tcacggtcgc cactgaggat gtgcagcgtc aggtttttgc ctgccaactg ccgcactgcc
    19021 tccgccgcgc tgtctttgag cgggtcttgc aggtagaaca cggcttggaa accgctttga
    19081 ctgccgaggt aaaccgcgct gccgccgcct tctgtttgcg gttctttacc tgaaatttcg
    19141 gcaacatagg atgccctccc caatgcccaa acctgtgttt cgccgttgac ggtcagttgc
    19201 gcacccacgc cttccccgat gcggttgagg cgttgtccga cttggatttc ggggacgctg
    19261 ccgcctgaaa tgcggcagtt gagaatggcg cgcgcgagcg ggtgttcgga ctgctgttcc
    19321 aaactttgcg ccactgccag cacttgggct tcggtcatgc tgccgagcag ttcaatccgg
    19381 cggacggcgg gattgccttg ggtcagcgtg ccggttttgt cgaaaatgat gtcggtggtt
    19441 tgggagaggg tttcgattgc ctgctttccg ccgattaaaa taccttcgcg cgccagcgta
    19501 ccggtagaag ctgccagcgc ggtcggcgtg gcaagcgaca gggcgcaggg gcaggtaatg
    19561 accagcaggg cgacggtaat ccacaatgcg gtgtgcgcat cggcgtacag cgtccagccg
    19621 atgaagacgg ggacggcaag caggagctcg ccgaatatga aagatgaggc gtattgttcc
    19681 gccaactcgg cagtacgcgg tttttgcgcc aaggcgcggt cgagcaggcg gacgatgtgc
    19741 gacaggcgcg tgccgccgcc ggtgcggtcg gtgcgtataa tcagagggct ttgcgtgttg
    19801 agtgtacctg cggttacttt ttcagacggc attttggcga cgggcaggct ctcgccggtc
    19861 agcatagatt cgttgacggc actgtttcct tccagcaccg tgccgtcaac ggggatggtt
    19921 tcgccgggtt tgaccatcac aatatcgcct gcttggagtt tgacgacggc ggattcgcgg
    19981 acatcctgta cggcaggata gccgggcatc cggtggcaga aggcgggtat cagtttcacc
    20041 agcctttcgg cggcatcgcc tgccttgcgt cgggcaatgt gttccataaa gcgtccgccc
    20101 agcaggaaaa acagcagcat cgcgatggat tcgaaataca tcccctgccc tgcgttggtg
    20161 gcgaggctgt aaatgccggc gatgaaggtc atgatgatgg cggcggcaat cggcgtgtcc
    20221 ataccggcgc ggcggttttt caggtcgcgc aacgcgcctt gataaaacgg gacggcgcaa
    20281 taaaatacga cgggcagcac catcaggaag ccgccccaat ggaggatctg caggaaatcg
    20341 ggttcgatgt cgccgccgta aaggtaggtc ggcagcgcga acatcatcgt ctgcatcata
    20401 cccagtccgg caacggcaag gcggacgatg tattgtttgc gttctttttg gttggcggct
    20461 tcgatttttt gcgcgtcata gggtgcggcg gtgtagcctg tctgccggat tttcaacaga
    20521 atgtcggaga ggcggatttt gccgtcgtcc cagacgacgc ggcagcggtg cgtgctgtaa
    20581 ttgaggtcga tgcggacgat gccgtctgtg cgcaaaagct gctgttcgat cagccagacg
    20641 caggcggcgc aggtaatgcc gctgagcatc agcactgctt cgtgcgtgcc attatgggtt
    20701 tccacaaagt cggattggac ttcgggcagg tcgtacaggc ggatttggtc gaggatttct
    20761 tggggcggca gttcggtttt tttcgcgtcg gcggtgcgtc gtttgtaata actgcccaag
    20821 ccggaatcga tgatgctttg tgcgactgcc tgacagccga cgcagcaggt ttcgcggtct
    20881 tcgccttcgt agcggacggt cagatgcagg ttttcgggaa cgtccagccc gcagtggaaa
    20941 caggtttttt tcatggcatt tcggtttcgt ctgtgtttgg tgcggcggca caatactcgg
    21001 caatggcttc gcgcagtgcg tctataccgg tattttcagc aacggaaatg cggacggcgg
    21061 caatttttcc cgcagcgtcg cgccatatgc ccgtgttttg ttcttcagac ggcagcaggt
    21121 cggttttgtt gtacaccttg atgcacggaa tatcgtgggc atggatttct tgcagtacgt
    21181 tttccacgtc ttcaatctgc tgtccgctgt tcggggcagc ggcatcgacg acgtgcagca
    21241 gcacatcggc ttgcgcggtt tcttccagcg tggcggaaaa tgccgaaatc agtttgtgcg
    21301 gcagatcgct gacgaatccg acggtatcgg tcaggataat gctgcatgcg ggactgatgt
    21361 acagccgccg cgccgtcgtg tcgagagtgg cgaaaagctg gtctttcgca tatatgcccg
    21421 acttggtcag ccggttgaac aggctggatt tgccgacatt ggtatagccg accagcgcaa
    21481 acgttttgat tctgcccgac tcgcgggact tgcggcgcag ggcgcgctgt tttttgaggt
    21541 tggcaagctg ttttttcaag gcgttgatcc gatgggcggt taatcggcgg tcggtttcca
    21601 gtttggtttc gcccggccct ttcatgccga taccgccgcg ctggctttgc aaatgtccgt
    21661 aaccgcgtat caagcgtccc gccaaatggc tcaattgcgc caactcgact tgcagcctgc
    21721 cttcctgcgt gcgggcgcgg cgggcgaaaa tcgccagaat cagccccact ctgtccaata
    21781 cgcggcattg gaggattttt tccaaattgc gttcctgcgt gggagtaagt tcgtggttga
    21841 atacgaccaa atcaatgccg tctgcggcaa ctgcttccga cagctccgcc gccttgcccg
    21901 tgccgacaaa cagcgcagtg tgcgggcggt cgcgtttggc agtctccacg cgtacggaat
    21961 cgccgcccgc cgctttgacc agctcgacgg cttccgccaa tgccgtctga aaaccgttca
    22021 gacgggcggc attgctgccc gtatcatctt tatccaacat tacgcccacc agcatgacgc
    22081 gttccggttg cgcctgagtg gcactgttcc gtcctgttct acctgacata actgtcaatc
    22141 ctgcaaataa agggttacac cgcgttccgg gctgtcttcc ggagtaagcc tgatgcggct
    22201 tcccgccatt ccgctgacgc tgaagacaat gccgcccgct tcgtgccgga gtatggacag
    22261 gctgttgccg aactgccgcc ggtaggtata acgggtacac gcttccagac accagcgtcc
    22321 gcgccagtgg atgaaatatt tttcgccttt tctaaccgca agtgtctcgc cgccgtttac
    22381 ctcaaaataa gggtaatcgg gttttcgggt tctaaactgc catttcgcct gcgcgcgccg
    22441 tccgttgcgg acatagtcga ataccgccgt ataaagcgtg ccgtattcca aaggtttgag
    22501 cgggaaaagg gcgaattggt gcgcggtcag cctgccgtta gggtcgttgc cggcggttaa
    22561 aaccctgacg gggcggattt cgtttttacc ctgatacagc ttgaaacttt tcatcgcaat
    22621 tttgcctgcc gcctcggaaa aatcaatgct ggcaggattg cctgtgattt catattccgg
    22681 cacggggtcg ggacgttccc cgtaaaaata aggcagcgcg ccgccgccaa ccggataggc
    22741 ggtataaagc aattccgtta cgggcatggc ttcgtcagca taaacggccg caccgttgtg
    22801 gcaagcgttg cggtaatatt tccgtcctgc ttccggctgc cgccttcctt ttgcacaggc
    22861 gcgctcgaag ctgccgttgc cctgattgaa tacgaggacg gttttgccgt tttcgcgcac
    22921 aaatgccgca cctgcttcgt cggtatggcg gtcaagcagc gaaaggcggt gatagacagc
    22981 actcatcaaa gcgtccactt ggcgttgctg cgtgcggatg tcgctgtcgg acgattcggc
    23041 ggcttcctct tccgtgctga tgttttcatg cacgccgttg tagagatacc cggcaaggcg
    23101 tgtgcgttcg gtcagctttt gtgcggtgta gtgcggattg tcgggatggt gttcgccgtg
    23161 tccgtcttcg ggattgagcg tgagatagcg tgcgtgcctg cgggcggaat tttccaaaac
    23221 cggcgcgtgt gccagcgcgt gcaaaccgat ttgtgtgcgg atgcggttga ggtagtgcag
    23281 cgcatcgccg ccgtccctga tttgcggtgc ggacggatag acaagttcgc ccgcgggcag
    23341 ggattggttt tgggtatggt agaaaacgcc tgccgccgag cccaatagaa gcagccaaat
    23401 aaaaagggat ttcataatgc cgccctcccg acgcggcaac ggggtgtttt atcaaaacgg
    23461 caaacttgcc aatgcggttt ccgcttgccg attgccaaac cggcaaacct ttcccgtgcc
    23521 ggtgccgaac ccgatgccgt ctgaaccttc agacggcatc gggtgtttat ttgtccactc
    23581 ggacgggtgc aggttcgtat tgtgtcgatt cgtcgccgta atacagcacg ccgagtttga
    23641 cggggatgcg tccctgcgat ttgcggtggg cgttggaatc gcgcaaggaa taggcgcagc
    23701 cgcagtattc ctgctggtag aagttttcgc gtttgctgat ttcaatcata cgcgcgccgc
    23761 cgccgccttt gcgccagttg aagtcccaat aggccacatc atcgtaaggc gcggcggcac
    23821 ggtgtccgca gtcgttgatt tgcgccatat ttttccagcg tgaaatgccc agcgaactgg
    23881 taaagacggg aaaaccgtgt tcgtgcgcgt attgcgccgc tttttcaaaa cgcatatcga
    23941 aacacatggt gcagcggatg ccgcgttccg gctcaaactc catgcctttg gctttggcaa
    24001 accattcttt gcggtcgttt tcgtagtcgt cgtctttatc gatgaaaggg atgccgaact
    24061 tttccgcaaa gcgcatgttt tcctcttttc ggagcatata ctctttgtgc ggatggatat
    24121 tgggattgta aaaataaatg gtgtagtcga tgccggaggc aagcatggct tccatcactt
    24181 cgccgctgca cggggcgcag caggaatgca gcaagacttt tttatgtccg ccgggcggga
    24241 cgagtatggg gcggtcaatg tcggtaacgg taggtttgtt ttgtgtttcc attaacttaa
    24301 caatatctgt cgaatacggt caaagcggct attttaccag agccgccgaa tgtaaaacag
    24361 ggtgtcaggg catcagcccg agctgcctga acgaacggac ggtttgcgag gtaacgataa
    24421 aatggtcgag cagcgaaaca tcaaccagcg acatggcctg tgccagccgc cttgtgaaca
    24481 tgatgtcttc ctgcgaaggt tcgggcgagc cgcccggatg gttgtgcgcg ataatcaggc
    24541 tgtcggcata ttcgtccaat gccagtttga cgatttcgcg gatgtaaatc gtgttttccg
    24601 caaccgtacc gcgcgacagc tctctgaccg caatcagttg gttttggcgg ttcagcagca
    24661 gcgcgacgct gacttcgact ttttcctgcc ccaaatgaaa gcgtaaataa tcggcaacgg
    24721 tatcgggatc ggataaggta atttcttcct gcaattcttc ctccaatatc cgccgcccga
    24781 tttccctgac cacggcgaac tgtgtgaaac ttgccgtccc catccctttg tatgccgaca
    24841 gtttgccgac ctccgcgctc atcagcctcc ccaagctgcc gaactcctgc aataggtagc
    24901 gcgccaaatc gaccgcgctc atcccgcgcg tcccgacgcg taaaaggatt gccaaaagtt
    24961 cggcatcgct caaagccgcc gccccgcgtt ccaacagctt ttccctgggc ctttcgcctt
    25021 ccggccattg cttgatgctc atgttttccc ttttttaaat agtctgagat gccgtctgaa
    25081 acagatgttc ccacacgcca aatatccgaa atgccgtctg aaccggattt ccgcttcaga
    25141 cggcatctgt atttcaaaac agtcttacgc cgtttcttcc aactcgattg aagccgccaa
    25201 aagcgacaga cgtgccatta cgccgtaaac gtagaggcgg ttatgttcgt tgtccacgtc
    25261 gcagtctttt tcagaacgcg gcataccgag cgaatcccat tgttcgaata cgcgtttgca
    25321 ggcttctgga gcttcttgcg aattatcgcc gttaccggta ggaatgctgt tggacagcgg
    25381 cacaaacacc ataccgccgg cgttgaggtt ttcgtccgca ccgcgccctt cgtgtacgcg
    25441 gaaaaagccg ccgatgacga aacggtccat catatagacg acgggttcgc acaccgcgcc
    25501 gttcaaggtt tcataggtat aaaccccttc ttggacaatc acttcgctga cttccaagcc
    25561 ctctttcacc ttcgccattt tgttgcggtt tttgcggttc aagccgcgca cttcgtcgga
    25621 ggatttgacg ctcatcacac ccatgccgta agtgccggca tcggctttga cgatgacgaa
    25681 aggtttgtcg gcaatgcccg attcgtcgta tttggcttga attttcgcca gcacgcgttc
    25741 taccgcttcc gccagcgcat cttcaccttc gcgcccttgg aagtccaaac cgccgatttt
    25801 ttcaaaatac gggttgatct gccattcatc gatgtcaatc agcttggcaa attcggcggc
    25861 aacttggttg tacgcgccga aatgatttgt tttgcggcgc gtcgtccaac cgccgtgcag
    25921 cggcggcaaa acggtttggc cgatgccttt gagaatgtcg ggaatgccgg cggacaaatc
    25981 gttgttcaac aaaaccacgc aaggcgaaaa gccgtcggca aggtggacgc ggtcgcgggt
    26041 tcgcagcaaa ggctcaagca ggattttgtc gcccaatgca gtttcaaatt cggccggttc
    26101 ggttacttcc ggattcaggc tgcccaagcg cacttcacac cctgccgaac ggaaaatctc
    26161 gccgagggcg taaacgtttt gcaggtaaaa cgtattgcgc gtgtggtttt cgggaataat
    26221 caatacggat ttctccgttt cgcaggcgcg ttgcaccgca tcttgcgcgg caaccgccgc
    26281 cagcgggatg aaattcggat tcagattatt gaagccgccc ggaaacaaat tcatatcgat
    26341 agacgaaatt ttgtaaccgg cgttgcgtat atcgaccgaa ccgtaaaacg gcgggcggtg
    26401 cgcgttccac tgcgtgcgga accacgcctc gattttggcg tgattggaca ggatttttgc
    26461 ctcaaacgcc tgaagttgcg ccgaatgttc gggcgacata accggtaatt tcatctttcg
    26521 tctgcctctg tgtgtttttt gacaaggtac agataataat gcttttgaaa gaatgacgac
    26581 aagtattctt tcgtatcgaa tcattcttta gatataacga aatgaacaat atgtctaatt
    26641 ttttcgttaa aattgtttac aaaataacac cgactcaaaa attagacaaa atctgttgcg
    26701 cagtataaag aatacgtcta aaatccgccc aatccgcatc accatttatc caaaagaaca
    26761 acatcatgac ggcacaaacc ctctacgaca aactctggaa cagccacgtc gtccgcgaag
    26821 aaggagacgg caccgtcctg ctttacatcg accgccatct ggttcacgaa gtaaccagcc
    26881 cgcaggcgtt tgaaggtctg aaaatggcgg ggcgcaagct gtggcgcatc gacagcgtgg
    26941 tctccaccgc cgaccacaac accccgaccg gcgattggga caaaggcatc caagacccga
    27001 tttccaagct gcaagtcgat acgttggatc aaaacatcaa agagtttggc gcactcgcct
    27061 acttcccgtt tatggacaaa ggccagggca tcgtacacgt tatggggccc gaacaaggcg
    27121 cgaccctgcc cgggatgacc gtcgtctgcg gcgattcgca cacttccacc cacggcgcat
    27181 tcggcgcact ggcacacggc atcggcactt ccgaagtcga acacacgatg gcgacccagt
    27241 gcattaccgc gaaaaaatcc aaatctatgc tgattgccgt tgacggcaaa ttaaaagcgg
    27301 gcgttaccgc caaagacgtg gcgctctaca tcatcgggca aatcggcacg gcgggcggca
    27361 cgggttacgc cgtcgagttc ggcggcgaag ccatccgcag cctttcgatg gaaggcagga
    27421 tgaccctgtg caatatggcg attgaggcag gcgcgcgttc cggtatggtt gccgtcgatc
    27481 aaaccaccat cgactatgtc aaaggcaagc ccttcgcacc cgaaggcgaa gcttgggaca
    27541 aagccgtcga gtactggcgc acgctggtgt ctgacgaagg cgcggttttt gataaagaat
    27601 accgtttcaa cgccgaagac atcgaaccgc aagttacttg gggcacgtcg cccgaaatgg
    27661 ttttaaacat cggcggcaaa gtgccgaatc ccgccgaaga aaccgatccg gtcaaacgca
    27721 gcggcataga gcgcgcactc gaatatatgg gtttgaaggc cggtacgccg ctgaacgaaa
    27781 tccctgtcga tatcgtcttt atcggctctt gcaccaacag ccgcatcgaa gacttgcgcg
    27841 aagccgctgc tatcgccaaa ggccataaaa aagccggcaa cgtacagcgc gtgttaatcg
    27901 tccccggctc cggtttggtt aaagaacaag ccgaaaaaga aggcttggac aaaattttca
    27961 tcgaagccgg ttttgaatgg cgcgaaccgg gctgttcgat gtgccttgcg atgaatgccg
    28021 accgcctcgc gccgaggcaa cgctgcgcct ccacgtccaa ccgcaatttt gaaggccgcc
    28081 aaggcaacgg cgggcgcacc cacctcgtca gccccgctat ggccgccgcc gccgccgtta
    28141 ccggacactt taccgacatc cgcacgatgg cgtaagtatt ttcaggcagt ctttaagaat
    28201 ttgacgaggc cgtctgaaat atgtcccgta aggcagaaac acctaatatt caaaccaagg
    28261 agtttaacca tgaacaaact tttcgttacc gccctgtccg ccctcgcctt gtccgcctgc
    28321 gccggcactt ggcagggcgc gaaacaagac accgcccgca accttgacaa aacacaggcc
    28381 gccgccgaac gcgccgccga acaaacaggc aacgccgtcg aaaaaggttg ggacaaaacc
    28441 aaagaagccg tcaaaaaagg cggcaatgcc gtcggacgcg gcatttccca tctcggcaaa
    28501 aaaatcgaaa acgccaccga ataaccgatt tgcgatgccg tctgaaaccg tttcagacgg
    28561 catcgtcagc acaagacgca aacccatgaa agcctttacc aaaatcaccg ccatcgtcgc
    28621 cccgctcgac cgcagcaacg tcgataccga tgccatcatc cccaaacaat ttctgaaatc
    28681 catcaaacgc agcggcttcg gccccaacgc ctttgacgaa tggcgttacc tcgaccacgg
    28741 cgaaccgggc atggacaacg gcaaacgccc gttgaacccc gatttttccc tgaaccagcc
    28801 gcgttaccaa ggcgcgcaaa tcctgttgac gcgtaaaaac ttcggttgcg gctcttcacg
    28861 cgaacatgcc ccttgggcat tggacgacta cggcttccgc gccattatcg cccccagctt
    28921 cgccgacatc ttctttaaca actgctacaa aaacggcctt ttgcccatcg tgttgaccga
    28981 agaacaagtc gaccggcttt tcaaagaagt cgaagccaac gaaggctatc ggctctccat
    29041 cgaccttgcc gagcaaaccc tgaccacccc gggcggcgaa acattcacat tcgacattac
    29101 cgaacaccgc aaacactgcc tcttaaacgg cttggacgaa atcggactga ccctgcaaca
    29161 cgccgacaaa attaaagcct ttgaagaaaa acgccgccaa agccagcctt ggctgtttaa
    29221 cggttaaaac ctatgccgtc tgaaggctgt tcagacggca ttcctgataa catataattg
    29281 ctgcggctaa tgtaaacaga ggggcgataa attgttttca tcttttaagc gggcttagca
    29341 acactctaaa ttaatgcaac tctccatttc aaataaattt ttagattttt aatatgcaac
    29401 aaattaaatt tattgactta ttttccggaa tgagtggcat caggaaagga ttcgaacaag
    29461 cctgtcggaa acagtcggtt gcttgcgaat gtgtttttac ttcagaaatc aaaccggcag
    29521 ctttggaagt attgaagcaa aactaccctg atgaagtgcc gtatggagat ataacgaaaa
    29581 ttgaaacagg ggatattccc gattttgaca tcctgttggc aggcttccct tgtcaggctt
    29641 tttctttcgc cggaaaaaga ttaggctttg aagatacacg gggaacgctt ttctttgatg
    29701 tggcaaggat tttaaaggca aaaaaaccaa aaggttttat tttggaaaat gtggaaggat
    29761 tggtgacaca cgatagaaaa gattcgacac aaaaaatagg gcgcaccctg accgttattt
    29821 tggaaacctt ggaagcattg ggctactatg tttcttggaa agttttaaat gcaaaagatt
    29881 tcggcattcc ccaaaacagg aagcgcattt atctgacagg cagtttgaaa tccaaacccg
    29941 acttatcttt cgaaacaagc ccaagtccga aattaaaaaa tattttggaa tcgggactgc
    30001 ctactgaaag cagccctttc atcaaaaaat tgctaaaaaa atttcccccg tccgaactgt
    30061 acggaaaatc agtaaaagac aaacggggag ggaaaaacaa tattcacagt tgggatattg
    30121 aattaaaagg cgcagtaacc gaagaagaga agcaattgtt aaatatcctt ctaaaagaac
    30181 ggaggaaaaa aaaatgggct tcagaaatcg gcatagattg gatggatggg atgcctttga
    30241 caaaagcgca aatttcaact ttctataaac accccgatct ccaaaatatt ttggacagcc
    30301 tgacagacaa aggctatttg gttttagagc atccgaaaca aaaaattggc gggcaaagaa
    30361 tcaaagatga atccctgccc aaaggctaca atattgtttc aggtaaaaaa tcttttgaaa
    30421 tcaataaaat attagatcca aacgatgttg cgccaacctt ggttgcaatg gatatggagc
    30481 accttttcgt cgttgacaac ggcggtttgc gtacacttac cggaaaagaa gggttacgct
    30541 tattcggcta tccggacgat tattcgtttg atattcccaa aaaagacaga tgcgatttat
    30601 tgggtaatac cgttgccgtc cctgtgatta aggcggtatc tgaaagactt ctgcatactt
    30661 tataaaccga cagggataaa atgataaaac ttactgcaca acaaatattt gataagttgc
    30721 tggatgaaga aaaaatctta tcggccaatg gtcaaattag atttttctta ggagatgtgg
    30781 atattatcgt caaacaaaaa gatgttgtcg gcaacatcat tcaggaatgg ctcggcggat
    30841 ggttgagaaa aagggaaatt gaatttgatg tttcaaccaa tacccaaatg cctcccgatt
    30901 tctttttaaa taaaaaagac cgcagcagag aattgttgga agtaaaggcg ttcaaccgaa
    30961 atgcctgccc gggttttgat attgcagatt ttaaaatgta ttctgatgaa atcattcata
    31021 agccctatat gctggatgta gattatttaa tattcggtta cgatatggac gacaacggca
    31081 atgtaaccat caaggattta tggcttaaaa aagtatggca aattaccaga agcatggatg
    31141 ggtgggcaat taatcttcaa gtcaaaaaag gcgtggtgca taaaatccgc ccgggtgttt
    31201 ggtacagcat aaataaaaag aatatgccta tgtttgaatg cttggaagat tttgtttccg
    31261 caattgaaga aaccgtttat caaaacccgg caacacggca taacgcatcc ttatggaaaa
    31321 agaaatttga ggaagcttat aaaaaacact acaaccgatc gatttccata ccccgttggc
    31381 acgaaattgc acacaaatac aaaaagaaat aacccttcaa ccatcaaccc ataaggaaac
    31441 ttgaaatgac caaacatatc gccatcctcc gaggcgacgg catcggtccc gaaatcgtcg
    31501 ccgaaaccgt ccgcgtactc gacaaattta tcgcccaagg cttggatgcc gactacgaat
    31561 acgcgccttt gggcggcgaa gcctacgacg aatacggcca tccttatcca gaatttacgc
    31621 aaaacctctg ccgcaaagcc gatgccgtcc tgcttggtgc agtcggttcg ccccaatacg
    31681 acaatctcga ccgtccgttg cgccccgagc gcggcttgtt agcaattcgt aaggatttga
    31741 acctgtttgc caatttgcgt cctgccgttt tatatcccga attagccaac gcctccacgc
    31801 tcaagcctga aatcgttgcc ggtttggata ttttgattgt gcgcgaactt accggcgata
    31861 tttatttcgg cgaaccgcgc ggcatccgtg ttttggaaaa cggcgaacac gaaggctaca
    31921 acaccatgaa atacagcgaa agcgaaatcc gccgcatcgc ccacgtggcc ttccaatccg
    31981 cccaaaaacg cagcaaaaaa gtctgctccg taggcaaagc caacgttttg gaaaccaccg
    32041 aactgtggcg cgaaatcttt gaagaaatcg gcaaacaata tcccgatgtc gagctttccc
    32101 atatgtatgt ggacaatgcc gccatgcaac tcgtgcgcgc gcccaaacag tttgacgtga
    32161 ttgccaccgg caacatcttc ggcgacatcc tctccgacga agcctccatg ctgaccggct
    32221 ccatcggtat gctgccttcc gcttctttgg acgaaaacgg caaaggcctg tacgaaccgt
    32281 ctcacggctc cgcccccgac atcgccggac aaaacaaagc caacccgctg gccaccatcc
    32341 tctcgcttgc catgctgctg cgttacagcc tgaacgacga agcgcgcgcg caacaagtcg
    32401 aaaactccgt ccaaaaagtg ctgcaacaag gcttgcgtac cggcgatatt tacgaagaag
    32461 gcacaaaact cgtttcctgc tccgaaatgg gcgacgcggt actcgccgcc ttg
//
LOCUS       DAXDSD010000012        32068 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXDSD010000012 DAXDSD010000000
VERSION     DAXDSD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32068
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.012"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(62..1855)
                     /locus_tag="ACFB2Q_000412"
     CDS             complement(62..1855)
                     /locus_tag="ACFB2Q_000412"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC0917521.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2101..3209
                     /gene="proB"
                     /locus_tag="ACFB2Q_000413"
                     /pseudo
     CDS             2101..3209
                     /gene="proB"
                     /locus_tag="ACFB2Q_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
     gene            3250..4485
                     /locus_tag="ACFB2Q_000414"
     CDS             3250..4485
                     /locus_tag="ACFB2Q_000414"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC0917522.1"
                     /translation="MPKRQKKSVNTADAEEKNRALLAMADSLEAAAEDILAANRLDLK
                     AAAGKIPDSMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPNGLEIVKKRV
                     AMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAALKTGLAQTRI
                     DPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPVIETGTGIVH
                     IYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLERLLVRGRIEA
                     GLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVGHIEARGTHH
                     SDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQKLHARGPIG
                     LKELTSYKYIVQGTGQVRE"
     gene            complement(4661..7705)
                     /locus_tag="ACFB2Q_000415"
     CDS             complement(4661..7705)
                     /locus_tag="ACFB2Q_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC0917523.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGAVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKTAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            7911..8591
                     /locus_tag="ACFB2Q_000416"
     CDS             7911..8591
                     /locus_tag="ACFB2Q_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917524.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTAALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNHYL
                     KSERGIWL"
     gene            8620..8979
                     /locus_tag="ACFB2Q_000417"
     CDS             8620..8979
                     /locus_tag="ACFB2Q_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFC0917525.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            9057..9163
                     /locus_tag="ACFB2Q_000418"
                     /pseudo
     CDS             9057..9163
                     /locus_tag="ACFB2Q_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(9584..10120)
                     /locus_tag="ACFB2Q_000419"
     CDS             complement(9584..10120)
                     /locus_tag="ACFB2Q_000419"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC0917526.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(10154..10510)
                     /gene="folB"
                     /locus_tag="ACFB2Q_000420"
     CDS             complement(10154..10510)
                     /gene="folB"
                     /locus_tag="ACFB2Q_000420"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC0917527.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            10566..11168
                     /gene="plsY"
                     /locus_tag="ACFB2Q_000421"
     CDS             10566..11168
                     /gene="plsY"
                     /locus_tag="ACFB2Q_000421"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC0917528.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            11260..12105
                     /locus_tag="ACFB2Q_000422"
     CDS             11260..12105
                     /locus_tag="ACFB2Q_000422"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC0917529.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLYAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DNKIR"
     gene            complement(12214..12546)
                     /locus_tag="ACFB2Q_000423"
     CDS             complement(12214..12546)
                     /locus_tag="ACFB2Q_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGK_0946 family protein"
                     /protein_id="HFC0917530.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(12596..12844)
                     /locus_tag="ACFB2Q_000424"
     CDS             complement(12596..12844)
                     /locus_tag="ACFB2Q_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917531.1"
                     /translation="MKMKKLILLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQI
                     SERAERAEYRKERREEMMDAARAIKKANENSPNIYFIR"
     gene            13087..14061
                     /locus_tag="ACFB2Q_000425"
     CDS             13087..14061
                     /locus_tag="ACFB2Q_000425"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC0917532.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(14220..14438)
                     /locus_tag="ACFB2Q_000426"
     CDS             complement(14220..14438)
                     /locus_tag="ACFB2Q_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC0917533.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLANNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            14632..14895
                     /locus_tag="ACFB2Q_000427"
     CDS             14632..14895
                     /locus_tag="ACFB2Q_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC0917534.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIGRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            15063..16831
                     /gene="ggt"
                     /locus_tag="ACFB2Q_000428"
                     /pseudo
     CDS             15063..16831
                     /gene="ggt"
                     /locus_tag="ACFB2Q_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            16895..17059
                     /locus_tag="ACFB2Q_000429"
     CDS             16895..17059
                     /locus_tag="ACFB2Q_000429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917535.1"
                     /translation="MSNQTKIQAIKQASEQILAICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            17004..17246
                     /locus_tag="ACFB2Q_000430"
     CDS             17004..17246
                     /locus_tag="ACFB2Q_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917536.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            17313..18563
                     /gene="glyA"
                     /locus_tag="ACFB2Q_000431"
     CDS             17313..18563
                     /gene="glyA"
                     /locus_tag="ACFB2Q_000431"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC0917537.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNETDARVLSNLVADVLANPE
                     DEANLAKVRGQVTALCDKYPVYGN"
     gene            complement(18636..19025)
                     /locus_tag="ACFB2Q_000432"
     CDS             complement(18636..19025)
                     /locus_tag="ACFB2Q_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC0917538.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
     gene            19965..20756
                     /locus_tag="ACFB2Q_000433"
     CDS             19965..20756
                     /locus_tag="ACFB2Q_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFC0917539.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPLSEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGASADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            20907..21551
                     /locus_tag="ACFB2Q_000434"
     CDS             20907..21551
                     /locus_tag="ACFB2Q_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC0917540.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            21570..23480
                     /locus_tag="ACFB2Q_000435"
     CDS             21570..23480
                     /locus_tag="ACFB2Q_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC0917541.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENKKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRNYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSRENKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            23502..23868
                     /locus_tag="ACFB2Q_000436"
                     /pseudo
     CDS             23502..23868
                     /locus_tag="ACFB2Q_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23980..25455
                     /gene="trpE"
                     /locus_tag="ACFB2Q_000437"
     CDS             23980..25455
                     /gene="trpE"
                     /locus_tag="ACFB2Q_000437"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC0917542.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFNNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            25539..26477
                     /locus_tag="ACFB2Q_000438"
     CDS             25539..26477
                     /locus_tag="ACFB2Q_000438"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC0917543.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDNWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            26478..27338
                     /locus_tag="ACFB2Q_000439"
     CDS             26478..27338
                     /locus_tag="ACFB2Q_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoMIV family type II restriction endonuclease"
                     /protein_id="HFC0917544.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCVYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            27355..28491
                     /locus_tag="ACFB2Q_000440"
     CDS             27355..28491
                     /locus_tag="ACFB2Q_000440"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC0917545.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKARIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            28538..29020
                     /locus_tag="ACFB2Q_000441"
     CDS             28538..29020
                     /locus_tag="ACFB2Q_000441"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC0917546.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            29126..30174
                     /locus_tag="ACFB2Q_000442"
                     /pseudo
     CDS             29126..30174
                     /locus_tag="ACFB2Q_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
     gene            30754..31359
                     /locus_tag="ACFB2Q_000443"
     CDS             30754..31359
                     /locus_tag="ACFB2Q_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC0917547.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEYDLDFSQAVG
                     QVSPLKNRQQYYAQSDKTPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            31422..31784
                     /gene="ssrA"
                     /locus_tag="ACFB2Q_000444"
     tmRNA           31422..31784
                     /gene="ssrA"
                     /locus_tag="ACFB2Q_000444"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         8278 a         8828 c         7485 g         7477 t
ORIGIN      
        1 tatcgccgca atgccgtctg aagccttttt atgcttcaga cggcatcgtc tgttttcacg
       61 ctcaaatcgt accgctgccc tgcgccttcg gtttggcggc actaaattcc agcttgctca
      121 aagcggaaag gtaagctttg gcggtcgccg ccaaaatgtc ggtatccgcg ccctgaccgt
      181 tgacgacgcg gttgccgcgc gccaaacgga cgctggtttc gccttggctt tccgtacctt
      241 gcgtgacggc gttgacggaa taaatctgca aagtcgcgcc gctttgcgcc acgctctcaa
      301 tcgctttgaa aattgcatca acagggccgg agccggttgc ggaagcgcgt ttttcttcgc
      361 ctttgatgcc gaacacgatg tcggcgcgcg gctcttcgcc ggtttcggtg ctgatttttt
      421 gagaaatgaa tttatagctt tcggcattca tattgcccat ttcgtccgac accagcgcgt
      481 gcaggtcttc atcgaagatt tcgcgttttt tgtcggcgag ttctttgaag cgtgcaaatg
      541 ccgcgttcag tgcctcttcg ctttccaact cgatgcccaa atccgccagc ttggttttga
      601 aggcgttgcg gccggacaat ttgcccaagc tcaatcggtt tgccgaccaa ccgaccgatt
      661 cggcagacat aatctcgtaa gtttcgcggt gtttcagcac cccgtcctga tggatgcccg
      721 attcgtgcga aaaggcattc gcgccgacaa tggctttatt gggctgcacg ggatagcccg
      781 taatggtgga caccagtttg gacgaaggca cgatttgcgt ggtatcgatg ccggtttcca
      841 agccgaacaa gtcgtggcgc accttcaacg ccatcacgat ttcttcaacc gaagcattcc
      901 ccgcgcgttc gcccaaaccg ttaacggtac attccacctg acgcgcgccg ccttttaatg
      961 cggcaagcga attggcaacc gccaagccca aatcgttgtg gcagtgtgcc gaccagacga
     1021 ctttgccgcc gttgggcgtt ttggcaatca gttcgcggaa aaattcttcg gttttgtacg
     1081 ggatggaata gccgacggta tcgggaatat tgatggtggt cgcgcccgct tcaattaccg
     1141 cgccgcagat ttcggcaagg aaatcgattt gcgaacgcaa cgcgtcttcg caggaaaatt
     1201 ccacatcgtc ggtgtattca cgagcgattt ttactgcttt gaccgccgcc tcaatcacct
     1261 gcttcggctt catcttcagc ttgtactcca tatggatggg gctggtggcg atgaaggtgt
     1321 ggatgcgttt tttcggcgcg ggcgcaacgg ccttacccgc ctgacggatg tcccgctcga
     1381 tggcgcggga caatgaacag accgttgatt tggtaatggt tttcgcaatc gcattgaccg
     1441 cctcgaaatc gcccgggctg gcggcggcaa aacccgcttc gatgatgtcc gcgcccaatt
     1501 tttccaactg gcgggcgacg cggatttttt cctctttggt catagcggcg ccgggcgatt
     1561 gttcgccgtc gcgcagggtg gtgtcgaaaa taataacgcg gttggcctgt gtcatttctg
     1621 ttctctccag agattcgttt ttttgtaaaa aagcattcaa atccagcggt cttccttgcg
     1681 attcggcaag cgcggtcaat ttttgcagtt gcgccaaagg cagcgagccg cgtgtgcgcc
     1741 atttgtagat ggcggcggtc gtcgcggcat tttcaggatc gtgccgtttc aacgcttcgg
     1801 caagcgcgtt cacgccgccg aaataggcga ccagacggtc tatatctaat tgcatggtag
     1861 tccttgtcta aaataaatca gcccgcagca gaaatatgga caggattata cgcattttgg
     1921 acaaaacggc aatattaatt tgaaaaaatc tcaataagtt gctataaatt ttattaattt
     1981 tatacatttt gtctatttta ttgcagatgc ggcgtgattg ttgacaattg cgacagaaca
     2041 gggcaaattc ggcggtacaa ggcggcaacg agacaggaca aaccaacaag gacggaaaca
     2101 atgaaataca aaagaatcgt atttaaagtc ggcacatctt cgattacccg ttcggacggc
     2161 agcctctcgc gcggcaaaat ccaaaccatc acccgccagc ttgccgcatt gcatcatgcg
     2221 ggacacgagc tggtcttggt gtcttccggc gcggttgctg cagggttcgg cgcgctgggt
     2281 ttcaaaaaac gtccggtcaa aatcgccgac aaacaggctt ccgccgccgt cgggcagggg
     2341 ctgctgatgg aagaatatac ggcaaacctg tcttcagacg gcatcgtgtc cgcacaaatc
     2401 ctgctcagcc gtgccgactt tgccgacaaa cgccgctacc aaaatgccgg cggcgcactt
     2461 tccgtgctgc tgcaacgccg cgcgattccc atcatcaatg aaaacgacac ggtttcggtt
     2521 gaggagttga aaatcggcga caacgacaca ttgagtgcgc aagtggcggc gatgatacag
     2581 gcagacctct tggtgctgct gaccgacata gacggtcttt acaccggcaa cccgaacagc
     2641 aatcccgatg ccgtacggct ggacaaaatc gaacacatca accatgaaat catcgaaatg
     2701 gcgggcggct cgggttcggc aaacggcacg ggcggtatgc tgaccaaaat caaagcggca
     2761 accatcgccg ccgaatccgg cgtaccggtg tatatctgtt cctcactcaa acccgattca
     2821 ttggccgaag ccgccgaaca tcaggcggac ggctcgtttt tcgtcccccg tgccaaaggt
     2881 ttgcggacac agaagcaatg gctggcgttc tattccgaaa gccggggcag cgtttatgtg
     2941 gacgaaggtg cggaacacgc tttgtccgaa caagggaaag cctgctgatg tcgggcattg
     3001 ccggaatcga agggcatttt tcccgtatgg acaccgtaac cgtgtacagc aaggcaacca
     3061 aacagcccct gggcaaaggg cgcgtccttt tcggctccgc cgccgccgaa gacctgctca
     3121 aatcgcgtaa ggcgaaaggc gtgttcatcc atcgggacga ttggatttcc atcacgcccg
     3181 aaatacgcct gcttctgacc gaattttaga aaaggaaacc catgtcaaac acacaaaaac
     3241 agcttgccct tgccaaagcg gcaaaaaaaa tccgtcaaca cggcggatgc ggaagaaaaa
     3301 aaccgcgcgc tgcttgctat ggcggacagt ctggaagcgg cggcggaaga tattttggcg
     3361 gcaaaccgtt tggatttgaa agcggcggca ggcaaaattc ccgacagcat gaccgaccgc
     3421 cttttgttgg acggcaaacg catttgcgcg atggcggacg gcatcagggc ggttgccgcg
     3481 ctgcccgacc ccgtgggcga aatactggaa acctcgactc tgcccaacgg cttggaaatc
     3541 gtcaaaaagc gcgtggcgat gggcgttatc ggcattattt acgaaagccg cccgaacgtt
     3601 acttccgatg cggcggcttt ggcactgaaa agcggcagcg cggtcgtact ccgcagcggc
     3661 aaagatgcat ttcaatccgc acgcgccatc gttgccgccc tgaaaacggg gttggcacaa
     3721 acccgcatcg accccgaagc cgtacagctc atcgaagaca cagggcgtga gggcagttac
     3781 gaaatgatga gggcgaaaga ttatctagac ctgctgattc cgcgcggcgg ggcagggctg
     3841 atacgggcgg tggttgaaaa tgccgtcgtg ccggtcattg aaacggggac gggcatcgtc
     3901 cacatttata tcgacaagga cgcggattgg gacaaggcac tccgtatcgt gtacaacgcc
     3961 aaaaccggcc gcccgtccgt gtgcaactcg atggaagtgc tgctggtgca tgaaggcatt
     4021 gctgccgact tcctgcccaa gctcgaacgg ctgttggttc gcggccgtat agaagccgga
     4081 ctgccgcccg tccgcttccg tttggatccg caggcggcgc ggtatatcgg cggcgaagcg
     4141 gcgggtgcag acgatttcga taccgagttt ttagactaca tcctcgccgt gaaaaccgtc
     4201 gcttcggttg aagaggcggt cgggcacatc gaagcccgcg gcacgcacca ttcagacggc
     4261 atcgttaccg aaaaccgcca cgctgcggac tatttcacga cccatatcga ttctgccgcc
     4321 gtgtatatca acgcgtccac gcgcttcacc gacggcggcg aattcggctt gggttgcgaa
     4381 atgggcatct ccacgcaaaa actccacgcc cggggcccga taggtttgaa agagctgacg
     4441 agttacaaat acatcgtaca aggcacggga caggttaggg aataatccga attcaaacgt
     4501 ctgatatagt ggattaaatt taaaccggta cggcgttgcc tcgccttgcc gtactatctg
     4561 tactgtctgc ggcttcgccg ccttatcctg atttttgtta atccactata aaaatgccgt
     4621 ctgaaacagt cttcagacgg catttttatt tgcaaaaaaa tcaagcattg tccaagggga
     4681 cgagaatcgt acggttgccg ttgtgttccg gtgcggacac aatgccttcc gcttccattt
     4741 ggtcaatcag acgcgcggcg cggttgtagc cgatacgcaa ggcgcgctgt acgcccgaaa
     4801 tgctggcttt gcgcgttttc aggacaacgg atacggcctc gtcgtacatc ggatcggttt
     4861 cgccgtcgcc gctgcgcccg ataccgggca gctcttcgct gccgccgccg ctcaaaatat
     4921 cgtcaacata gtccggctcg ccaaactgct tcagatattc gaccacgcgg tgcacctctt
     4981 catccgaggc aaacgcgccg tgaacgcgct gcggataggc agtacccggc ggcaggaaca
     5041 gcatatcgcc ctgaccgagc aggttttccg cgcccatttg gtcgagaatc gtgcggctgt
     5101 cgattttgct ggacacttgg aacgcgatac gcgtcgggat gttcgcctta atcagacccg
     5161 tgatgacatc gacgctgggg cgttgtgtgg caaggataag gtggatgcct gccgcgcggg
     5221 ctttttgggc gaggcgcgca atcagttctt cgattttctt gcctgccgtc atcatcaaat
     5281 cggcaaactc atcgaccacg accacgataa acggcagttt ttccaaaggt tcgggatcgt
     5341 cgggcgtgag gctgaacgga ttgccgattt tttctcccct tgctgcggct tcggcggttt
     5401 tttggttgaa gcccgcaaga ttgcgcacgc ccataaagct catcaggcgg tagcgttttt
     5461 ccatttcgtt aacacaccag ttcagcgcgt ttgccgccag cttcatatca gtaacgacag
     5521 gggcgagcag gtgcgtgatg ccttcgtaaa tgctcaattc cagcattttc ggatcgatca
     5581 taatcatacg cacgtcttcc ggcgcggctt tgaaaagcat agacagaatc atcgcgttga
     5641 cacccaccga tttgcccgaa ccggtcgtgc cggcaaccag caaatgcggt gctttgccca
     5701 agtcggttac gacgggctgt ccggtaatgt cctgaccgag cgcgagcgtc agcttggatt
     5761 tggattcggc aaactcgggc gaattgaaaa tttcgctcag gcgtatcatt tggcgtttcg
     5821 ggttcggaag ttccaaaccc atgcaggttt tgccggggat ggtttcgaca acgcggatgg
     5881 aagccacgcc gagcgaacgc gccaagtctt tttccaaatt cagaacggaa ttgccgcgca
     5941 cgccgacatc gggttcgatt tcataacgcg taatcacggg gccggaataa gaatcgacaa
     6001 ccttgacctt gactttgaac tccgccaatt tttcttcgat ggtgatgctg ttttccaaca
     6061 gttcttcttc ggtttgcgtc gcctcgggat tgaacagcgg aggcagaagc aggtcggttg
     6121 tcggcaggtg ttcggatacc gcaccggtct cttccgattg gaacgcccct tcatccgctt
     6181 ccgtatccga tacccggcag gacgggcgtt cagacggcac atcttcaaac ggacaaaccg
     6241 cctgactgtc atgcccgaac gcttcggttt ccgacagata ttgcccgctt gaccgctctg
     6301 ccgcaccctc actgccgtca tctgcaatag cggcggtttc ctcctgccaa cctccattca
     6361 aaacatcagc ggcaagatgg tcggtttcgg caatgcggct gcgttgcgcc tgctcgaatc
     6421 ctgccggcgg ctcataggta cggttgtaga tttccgatac gggaggcggc ggcagaatat
     6481 cgatttcggg tacggctacc tccggcactt cgggtggttc gaccacccaa gcatcgggcg
     6541 cggcaggctc ttcaatatgg atatcggcag cttcatcata aaccggaccg ccgtatccca
     6601 cagacgaaac ggtttccgtg aaaacatttt cggtttcaga cggcatttcg acatcccgtt
     6661 tcccgacaac cgtccgactt tccggaatca ggcgcgaaat ccgcgcctcc gcagaaaccg
     6721 ttgccttgtt cggttctttc aaattgacgg aaacgcggcg gcgggcatcg cggacggctg
     6781 tgccgtctga aacggactgc ccttgggaaa catccgggga ttttgcctcc gcctgccctt
     6841 tctcctgccc tgcatgacgg cggaacggcg cattccgtgc attttcggct ttgactgcag
     6901 aaaaggcaga cggatgctgc ttctcaaaat acggctcaaa tccgtaatcc gcagactcgg
     6961 aaaacgcttc tttgtccgca tcaaacatac gggaatacgt gcgttgaagc acagggtcgt
     7021 ccaaaccgat aatctgcagc ccttccatcg gggtatcgga cacgcgtact ttggggacgg
     7081 ctgttccgtt tttctcaaat gcatcgatat agcgtttttt cgtttccctt aaagccgcgc
     7141 tgcttaaagc acgcgttgct tcttccaaag tgatttcctt aaaaaccgga cggacgggcg
     7201 aagttttgct ttcagactgc atcaaccctt cagcaatact ccggtcgaat gggatgcggc
     7261 ggttgtcgat tacggcagtt gcaatgtctt ccgtatcggc agcctcttcc tccgcagctt
     7321 ctgctgcttc cgtttccgct tcttccgtcc cgtttccact gtctgaatac ccgtcggatg
     7381 cggtcggaac atccgcacgg tttgcagaag caggcggaac gggcctgtcc ggttcggatt
     7441 cgtgcgccag atagtttcgg cagaaacgga cggcaccgac aaacagccaa tacactgccg
     7501 ttttgacggc atggaaaagc atcagggaaa actcgggaaa atcgggcata ccgtctttga
     7561 tttcaggcag ctccgcctgt ttttcccctt tcttttcctg ccacgcagaa acctcgcgca
     7621 tccactcgcg ttcggattgt gcgcggacaa aaaacaggcc ggcaagcgca agcaacaaaa
     7681 taacgatcaa aactatccaa aacatatgcc gtttcccgca agacaaacga ttaaccgagt
     7741 ttattttaac gtgtttccag cccaaaaaaa acaaccggcc ggcaattctg ccgaaaccga
     7801 ttcggctttc ggtttgcccc cgccgacagt ataatagccg ccccttccct taatctttgc
     7861 ggcattacca tgattttcca aacagtctgg ttttcatttg cggcattacc atgattttcc
     7921 aaacagtctg gttttcagat gtggtactgt cggtttcgtg gattgtcctt atcctgatac
     7981 tggcagcttc cgcgccgtcg gcattccgtt cgcttgcccg ctaccgttcc gcccttcccc
     8041 tgtgtaccgt aattttttcc gcagcatggt gtctgaacgc ctctgtcggc ggcggacagc
     8101 ttgcccagat gaactaccac ctgcttgccg tcaatctggt tacactgatg gtggatacct
     8161 ccgccgccct ttggcttgcc gcgctgctga tgctgcccta ctgcctgctg tttgccggtt
     8221 ctgccggcgc atatccgccg aacgctttgg tgctgatact gcccgcgctt gtcgtcaacc
     8281 gcctgtcgcg tatgctggtc aaccgtctgc cgcccaatat tttcatcttt attttcgtca
     8341 acggctttct cgcttccgcc gccggcattc tgctgaccgg gctggtgctg atcggtattt
     8401 tggatgccgc aaacgctttt ccgtccgaaa tattgtggac ggccgccctg cccgtcttta
     8461 ttctgctggc gtgggcggaa gccttcctca gcggcatttc aactgccatt ttcgtggcac
     8521 tgaagcccca ttggatcaac actttcgacg acaaccacta tctgaagtcc gagcgcggca
     8581 tatggctgta gccttcagac ggcataggca gacaaaacca tgctttccaa catcctcccc
     8641 ctttccatcg gtgcaatatt cggcacaacg gcgcgttggc tgctcaacct cgccgtcccc
     8701 gcatcgttgt ctcccgccac aggcaacctg tttgccaact ggacgggcgc gctgctcatc
     8761 ggcatctttg ccgaaaccgt cagccatccg caatggaagc tgctgctgat taccggtttt
     8821 ttcggcagcc tgaccacgct ttccggattt tcactggaaa ccgtaaccct gctccaatcg
     8881 aaccgtcccg cttcggcact tgccaatatt ttcctacaca cggcaggttc gctgctgctg
     8941 acttggctcg ggctgaagat agggacggca gtcaaataac cgcaaaaaat tgcttccttt
     9001 ggttttgaca taatctataa attggcttcg cttgtttaaa tactgcctgt gccaattata
     9061 gtggattaaa tttaaatcag gacaaggcgg cgagccgcag acagtacaga tagtacggca
     9121 aggcgaggca acgccgtacc ggtttaaatt taatccacta taccgttctc aaattcaaat
     9181 tgggctttta ggtctataac aaagaaaaag ccttgggagg ataaacctac caaggctttt
     9241 tcttaaaaaa cgctctatca ctgtgcctaa aaatgcaaaa aagtaaccgt cataatcctt
     9301 ttgccaaaat caatatcatc atccgatgaa tgaacggacg gtatataaga aagcgttaat
     9361 aaactcataa accagtaaga aaaatcagta acgaagatat tcattattac ccattcttaa
     9421 aaattatcac gttcgtttgg gtaattatca tacccttttc cctcttatta ctaaagcacg
     9481 aattacttta aagtacgcac ccagagacaa gccgaaaagg catatccgat ccgccaaaca
     9541 aaatgcccgc cgacttactc cggcgggcgg ttccaacatc ctgtcaatcc ttcatcagcc
     9601 agtattgcaa accgattaat gttttaccgt ctttaatttc atcgtttgcc aatgcctgac
     9661 ggacttcatc tttcgacatc agcacggttt ccgttatttc atcttcatca ttagaaagcg
     9721 tactgcccaa gcgcacgcct tccgcctcga acagatacat tttttcattg caaaaaccga
     9781 ccgccgtata aaaactgtac agcaggcgca cgctgtcggc aacataaggc gtttcctccg
     9841 ccaattctcg cagcgcacac gctgccatat cctcgcccgc cacatccagc ttgcccgcag
     9901 gaagttccaa tgtcgcctga tttgccgcat aacgccactg ccgcaccaaa acgacctttt
     9961 cgtcatccgt taccgccaac acgcacgccg cgcccggatg ccggatgacg atgcgctgcc
    10021 cttcattgcc gttgggcagc ctgaccttat ccctgctgat actgacgaaa ccgccctcgt
    10081 aaatggtttc gccgcctaat tttacttccc tcaaatccac ttcatatctc ctttccgttt
    10141 cctattctgc cgttcaatct tcacgcttgc cgcgctcgat ttccactcca acctcgcgca
    10201 cgccttccaa aatacccggc ttgacgattt tcacgtgcac ccacaccgcg ccgaaatatc
    10261 ccaaaaccaa atcggcaata tattccgcca acgcttccaa aagcaggaaa tcctgttctt
    10321 tcagatgtcg gcgcagcgtt tcgcatacct cggcataatg caccgtattg gcaatatcgt
    10381 cgtccgaacc cgctttctcg ggaacgccga tgtccaaatc gacaatcagg gtctgcaacc
    10441 gttcgcgttc ccagccgtac acgccgataa gcgtatccgc cttcatgccg tgcaaaaaga
    10501 ttttatccat aatccgcccc gttttttgct aaaataaccg caccattcta agtaaagtac
    10561 cgcaaatgtt caacataccg gccgtcgccg tttcctattt aatcggttca ctttctttcg
    10621 ccgtcatcgt ttccaagtat tacgggatgg acgacccgcg cacctacgga tcgggcaatc
    10681 ccggcgcgac caatgtttta cgcagcggca aaaaaaaggc ggccgcgctg acgctcttgg
    10741 gcgatgccgc caaaggtttg gttgccgttt tgcttgcacg cgtgcttcaa gaaccgctcg
    10801 gtttatccga cagcgcaatc gccgccgtcg cactcgccgc gctggtcggg catatgtggc
    10861 cggtgttttt cggatttaag ggcggcaaag gcgtggcaac ggcattgggc gtgcttctgg
    10921 cactctctcc tgcaactgcc ttggtctgcg cgttgatttg gcttgtgatg gcattcggct
    10981 tcaaagtatc ctcccttgcc gcgctggtcg ccacaaccgc cgcccccctt gccgcactgt
    11041 tttttatgcc gcatacttct tggattttcg caaccctcgc aatcgccata ttggtgttgc
    11101 tccgccataa gagcaacatc ctcaacctga ttaaaggcaa agaaagcaaa atcggcgaaa
    11161 aacgctgatt cggcatttgt tttccattac gtcatgccgt ctgaaaccat gttcccgcgt
    11221 tcagacggca tttccgcaca aacggctttc atacatacca tgctcagtta cagacacgca
    11281 ttccacgccg gcaaccacgc cgatatgctc aaacacttca ccttgttttt agtgctgcaa
    11341 tatttcaacc gcaaagacaa gccctactgg tacatcgaca cgcacggcgg cgcgggtttg
    11401 tacaatctcg aaggcagcga ggcgcagaaa gtcggcgaat accggcaagg catcgccctg
    11461 ctccgacagg cgcaaaacct gcctgccgaa ctctctgact ttgccgcgca catacaaaaa
    11521 atcctaccct cgcccgaact ttactgcggc tctccgtggc tggcgcaatc gctgatccgc
    11581 atcggcgaca aattgcgcct gtttgagctg caccccaccg actttgtcca tctgcaaaac
    11641 aatatgggcg aagcgggatt gggcaaacgc ggacaagtgt tgcgcgaaga cggttacaaa
    11701 gggctgattt ccctgctgcc gcccccgccg cgccgcgccg ccgtcctcat cgacccgccc
    11761 tatgaggaaa aacaggacta ccggcgtgta acggaaacgc tgaaagccgc tttaaaacgg
    11821 tttgagtccg gctgttatct catttggtat ccctgcctca gccgcgaaga aagccgcaag
    11881 ctgccggaag aattgaaaaa actcctgccc gacaactacc tttacgccga actgcacgtc
    11941 cacgcgccga aaaccgacgg tttcggtatg cacggcagcg gaatgttcgt gattaatccc
    12001 ccttaccttt tagccgaaca attggcggca aacctccccg ccctgacacg gctgctggcg
    12061 caagacgaag gcgcgcgcta cctgttggac aacaaaatcc gctgaccccg atgccgtctg
    12121 aaacccttca gacggcatct tccgtcaaac aaacaaaacc gggcgataca cactccatat
    12181 cgtccggttc tgcataaaac cagtattcaa aaatcagaac acgcttttca ttacacccac
    12241 ggtaccaacc acaccgttca gcagcatcag accaccggca gccgtaccca taccttgacg
    12301 gattgcgtca tgcttattgg cacgtttttc acgttccaaa gccaactcct cagaaacatt
    12361 tttacgttga cggcggtgtt gcgccagttc cgcacggcta attcgcgctt cggcacggtt
    12421 acgaccagcc ttgattacgg ccgaatcgtc gccgatagca tagttggcta cagaattttc
    12481 agaagcgcag gcagcaagca aaacggcaca cacagcaaca acggaaatat ttttcaagtt
    12541 tttcatacct gtcctattat ctcaaaataa agaaaaaact aatccaactg gagttttatc
    12601 ggataaaata aatattgggt gaattttcgt ttgccttttt gatggcgcgt gccgcatcca
    12661 tcatttcttc ccggcgttct ttacggtatt cggcacgctc ggcacgctcg cttatctgaa
    12721 tggcacgact tttcgcctgt atttccgctg cagattcttg agttacaaaa cggcggtctg
    12781 cgtaagtaca tgccgtcacc aacatcgcag ccaccgacaa caaaatcagt ttcttcattt
    12841 tcatctacct atatttaagt ttatttgtaa aatatgttat cactatatat atatataatg
    12901 tcaatatttt ttaaaaataa atttggcatc ttgtacttct cgatccgcct gccgcgttcc
    12961 gacttgcccc gcgccgtttt tttccactac aatcccacgc gatatccggc gaaacgcacc
    13021 aaaggaacgc cccgcagcaa tctgccgcct ttcagacggc acaaaccttt ccgaaacgag
    13081 caaaccatgg acacactgac ccgattcctc cccgaacacc tgcaacaaaa ccaactgccc
    13141 gaagcactcg gcggcgtttt gctgtccgtc gtttccgcct gtaccgaaat caacgccaaa
    13201 gtccgcctcg gcgcacttgc cggcgtattg ggtatggcgg gcacgggcaa tattcagggt
    13261 gaagaccaga aaaaactgga tgttatcgcc aacaacatta tgattgacac actcaaagcc
    13321 aaccctgccg ttgccggttt ggcaagcgag gaagaagaca ctttcgtaag tgccggtgaa
    13381 aacggacgct atctcgtcct attcgaccct ttagacggat cgtccaatat tgatgtcaac
    13441 atttccgtcg gtacgatttt ctccattctc gccaaacccg aaggcgcatt ggcaaccgaa
    13501 tcattccttc aaacgggcag acagcagctt gccgcaggct atgtcctcta cgggcctcaa
    13561 acccagctcg tattcacatt cggacacggc gtgtacgtat ttacactcaa tgccgaaaac
    13621 gaatttgtgc tgaccaaaga aaacccgaaa gtacccgaaa gtaccaaaga attcgccatc
    13681 aatatgtcta accgccgcca ctggctaccc cccgttcaac aatacgtcga cgagctcttg
    13741 gcgggcgaaa ccggtacgcg cggcaaaaac tacaatatgc gctgggtagc cagtatggtt
    13801 gccgaaatcc accgcatcct gatgcgcggc ggcgtgttca tgtatctgca agacaaacgc
    13861 gacccgtcca aacccggcaa actgcgcctg atgtacgaag ccaaccctat ggccctgata
    13921 ctcgaacagg cgggcgcatc ggcaagcaac gcatatcaag ccatgctcga tatacagccc
    13981 gaaagcctgc accaacgcgt cgccgtcatt atgggcagca gcgaagaagt ggattacctc
    14041 aaccgtctgc attcaaaata ggcagagacc gtactgataa agaccggcgg cagcgtgaat
    14101 ccgatactgc gccgatattt caagcatacc ctccggaaaa acagaaaact gcctttccga
    14161 acgataaaaa taccgtctga aaaattttca gacggtattt taaaaattag agctcaaatt
    14221 tatttgcccg ccgctttttg caggatggcg tacaactcgt ctttcagttt caatttggct
    14281 tttttcagct catcgatggt ttccgcaccg ctggtaaccg gattgttggc cagaccggta
    14341 attttatcgt ccagctcgtt atgctcgtcg aacagacggg cgaagcggga attttcctgt
    14401 ttcaatttgg aaatcaaatc acggtattct gggaacataa tcgctttcct ctcttggttt
    14461 acggttaaaa agtcaaattg aatctttaca ttaattataa caaagataat atttttatcc
    14521 agtgttttgt tatacacggc aacaccgtcc gcagaaaacc gccccaccgt ccgataaaat
    14581 cgttgacaat cttccgcttt acgcataaat tttcagccct tcctactgca tatgaaaggc
    14641 acgccccagt ttccccaatg tggattttca gcgcgcgcag tagaggtgcc gacccaaatt
    14701 ggcagaccct ttgcttttgt taatatccta gaaaatccag aaattcgcag tactctacca
    14761 ctcatcgcca attggccaac ctttccacgg ctttgggtta atggtgagtt aatcggtggt
    14821 agtgatatta tcctacaaat gtaccaatca ggtgagctaa aaccactcat tgaagagcat
    14881 agcccaaagg tctgattact taatatctta aaataaatat caaaaaagac tgctgatgac
    14941 attagcggtc tttttttgat ggtcaagatt gatagtttgg attgaattat ttagccatat
    15001 tacatacaat aatcagcatg ctttgtatga attatcaatc aaactcaggc gaaggagtgc
    15061 ttgtggctaa aacatattta ttgactgcat tgataatgtc tatggtaatc tccggatgtc
    15121 aagtcatcca tgccaatcaa ggtaaggtta atactaattc tgctgtcatc gcaggtgcag
    15181 acgctcacac gcctgaacat gtaacgggac tgaccgaaca aaagcaggtg attgcaagtg
    15241 attttatagt agcgtcagcc aatccattag caacacaagc tggctatgat atcttaaagc
    15301 aaggcggtag cgctgcagat gcgatggtgg cggtgcagac gacactaagc ttggtagagc
    15361 cacagtcgtc aggcttgggc ggtggtgcat ttgtgttgta ttgggacaat accgccaaaa
    15421 cattgaccac atttgatggg cgtgagacgg caccgatgcg tgcgacgcca gaattatttt
    15481 tggataaaga tggttaacca ttgaaattta tggaagcggt ggtcgctcgg taggtacgcc
    15541 tgctatccct aaactgacgg agacaataca tcagcgatac ggtgtattgc cttggggaaa
    15601 attatttgat acgccgatcc acttggcaaa acaaggcttt gaggtgtcgc caaggcttgc
    15661 catctcggtt gagcaaaatc agcagcattt ggcacgctat ccaaaaacag ccgcttattt
    15721 tttgccgaat ggtgtgccgc tacaagcagg cagcttgctg aaaaatttag aatttgctga
    15781 cagtgttcag gcgttagcag ctcaaggtgc aaaagctctg catactggta aatatgccca
    15841 aaatattgtt tcagttgtcc aaaatgctaa ggataacccc ggtcaattat ccttgcaaaa
    15901 tttatctgat tatcaagtgg tggaacgccc gcctgtttgt gtgacttatc gtatttatga
    15961 agtatgcggt atgggtgcac caagctcagg tgggattgct gtgggtcaga ttttggggat
    16021 tttaaatgaa ttttcaccca atcgggtggg gtatgatgct gaaggtttgc gtctcttggg
    16081 cgatgcttct aggcttgcgt ttgcggatcg tgatgtatat ttgggcgacc ctgattttgt
    16141 accagtaccc attcgccagt tgatttctaa agactaccta aaacatcgca gccagctgct
    16201 tgagcagtcg gataaggcat tgcctagcgt atctgcaggc gattttattc atgagtgggc
    16261 gtcctcacaa gcgattgagt taccttctac cagtcatata tcaattgtgg ataaggcggg
    16321 caatgtgcta tcaatgacga cttccattga aaatgcgttt ggctcaactt tgatggcaaa
    16381 tggctacctg ctgaataatg agctgacaga ctttagtttt gagccaataa agcagggcaa
    16441 acaggtcgcc aatcgtgtcg agcctggcaa gagaccacgc tcctcaatga caccaaccat
    16501 cgtatttaaa gcaggcaagc cttatatggc aatcggctct ccaggtggta gccgtatcat
    16561 tggctatgtc gctaagacga ttgtggcaca tagtgactgg aatatggata tccaagatgc
    16621 catcagtgca cccaatttat taaatcgctt tggtagctat gaactagaaa cgggtacaac
    16681 cgccgtccag tggcaacaaa cattaaatga tttgggctat aagactgatg tgcgtgagct
    16741 aaattcaggt gttcaagcca ttattatcga gccaagcagg ctggttggtg gtgtcgatcc
    16801 acgccgagaa ggtcgagtga cgggtgatta gaggtgaagc ttgtattttg tgaatttgac
    16861 cgcattaagt taattattaa aataggggga aattgtgagt aatcaaacaa aaatacaagc
    16921 catcaagcaa gcttctgagc agatcttggc aatttgtgag acgccaaaca cagcattaca
    16981 agccattcat ctgattttaa gacatggcgg ggcaggaaga attgtcttgg caagtggtgt
    17041 atcagcgggt tatggctgat aaagatgtgg ttggtgctgg ttatttgatt gattttgcac
    17101 aaactgccga aaatttacca tttgatgtat tgcctttgat tagccttgtg ctaaacaaag
    17161 gtgatgagac gctaaaaacc ggcatgttaa ataaattacc tgataatgcc aaagaaaatt
    17221 tacgcattat gggctatctg ccttaggttt tgaagtattt ttttatacga aaaacgctca
    17281 tgataccctg attacacaaa ggagccgaga atatgttttc aaaaagcgtt accctcgcac
    17341 aatatgaccc cgatttggca gcagccattg cccaagaaga ccgacgccag caagaccacg
    17401 tcgagctgat tgcctctgaa aactacgtca gctgcgccgt gatggaggca caaggttcgc
    17461 aattgaccaa caaatacgcc gaaggctatc ccgcaaaacg ctactacggc ggctgcgaat
    17521 acgtcgatat tgtcgaacaa ttggcaatcg accgcgtaaa agaactgttt ggcgcagcct
    17581 atgccaacgt tcaaccgcac tccggttccc aagccaacca agccgtatat gcttccgttt
    17641 taaaaccggg cgacaccatt ttgggtatgt ctctggcgca cggcggacac ctgacccacg
    17701 gcgcgagcgt taatatttcc ggcaaactct acaatgccgt tacttatggc ttggatgaaa
    17761 acgaagtcct cgattatgcc gaagtcgaac gcctcgcgct cgaacacaaa cccaaaatga
    17821 ttgtggcggg cgcgtctgcc tacgcgttgc aaatcgactg ggcaaaattc cgcgaaatcg
    17881 ccgataaagt cggcgcatac ctctttgtcg acatggcgca ctatgccgga ctggttgccg
    17941 gcggcgaata tcctaacccc gtgccattct gcgacttcgt aaccactacc acccacaaaa
    18001 ccctacgcgg ccctcgcggc ggtgtgattt tgtgccgtga caatacccac gaaaaagcgc
    18061 tgaactcttc catcttccca agcctgcaag gcggtccgtt gatgcacgtt atcgccgcca
    18121 aagccgtagc gtttaaagaa gcgttgcaac ccgagttcaa acaatacgca aaacaagtga
    18181 aaatcaatgc tgccgtcatg gcggaagagt tggttaaacg cggtttgcgc atcgtttccg
    18241 gccgcaccga aagccacgtt ttcctcgtcg acctgcaacc gatgaaaatc accggcaaag
    18301 ccgccgaagc cgctttgggc aaagcgcaca tcaccgtcaa caaaaacgcc atcccgaacg
    18361 atccggaaaa accgttcgtt acctccggca tccgcatcgg ttccgccgcc atgaccactc
    18421 gcggctttaa cgaaaccgac gctcgcgtat tgtcgaactt ggttgccgat gtattggcta
    18481 atcccgaaga cgaagccaac ctcgccaaag tccgcggaca agtgactgcc ttgtgcgaca
    18541 aataccccgt ttacggaaac taaacgcccg atgtttccat acaaaatgcc gtctgaacat
    18601 tttccagacg gcattttttg ttttcggtaa cattgctatt ttttctcttc cagcaaaatg
    18661 accaactttt tcaatactgc gatttcacta tctttctgcc tgatcaattc atcctttgca
    18721 tccagcagcg acctcaattt ttcattttca aacaccagca tttcatcaga gccgataata
    18781 ttatgatggt tattggtatt gtcgccgata gagaagaaaa acgatcggtt ctgctccgcc
    18841 agcagctcaa ccacatcgat attgaaaatc tgggcaatct ggcgcagctt gtcgagattg
    18901 ataccgcttt tcccgcgttc tattttcgaa tacccgttaa ccgacatttc caatttttcc
    18961 gccatctctt cctgcgtcca ctgattgact tcccttaacg tccgaatctt gtcatgcact
    19021 tccatcctgc cctccaaggt taattttcac cactcaaaca accgtaaccg gtatcttcag
    19081 ttgatggtcg gatattttaa aaataaataa tataacacaa tagtattttg ataaaaattt
    19141 acctaacgag gtacagacat gtattcagaa aacaagccta aacgcaccgt aaaaatgaaa
    19201 aaatactggc agcctgcacc ctttccctgt ccctttctgc ctgtacttcg actcaagtaa
    19261 cttcttttgt cgagggactc tccgagggac tcgtagatgt cagcgaagcc tttatcacaa
    19321 caaaggcaga atcatattct gccccgtctc ttcaggataa gcaaattttc ataaatcgcg
    19381 tctcagaacg agcacaaaaa aattacaaag aaagaaggaa aaagaaagaa gcagacttac
    19441 taaattcagc atcgtcatta aatgaatcaa catcagaagc cctatctgcc cttaaaaacg
    19501 ctatcgtaat tcctaaacgc cctaaacgct ccttgggcaa aaaatataaa aacggttttc
    19561 atcctgtaaa tacgtgtgcg gatgtggaag ctcggtgggc aaacggagca atgagagatg
    19621 ttagggacat agaaaggcta ataagccgaa acgtcccttt acgtcgtatc ggagacgcca
    19681 ttcaaaacat gtgcatggaa accaatgtgt ccattaggat gtatcgccag gagtgccgtt
    19741 cagaatcgtc agatttttat gacgaaatgc ttgagcatca cgagaatttc ataggatcct
    19801 ccaaatcatt cgcatcatca cttcttcccc ttagcgttac aaactttaca acagacatgg
    19861 cgtgtactcc cgaattttaa tctaatcaca ctccacaact ccctatcaat ataactgcta
    19921 cttctctgag tagtatcaac caacccttaa caaaggacta aaaaatgaaa aaagcactgc
    19981 ttgcactgac tattgccgcc atctccggta ctgctatggc ccagttgccc gactttctgg
    20041 gtaaaggcga atataccgtc cgtacagaca tctccaaaca aacgctgaaa aatgccgatt
    20101 tgaaagaaaa acacaaagta caaaaaaaca tcggtttccg tgccgatatg ccgtttgacg
    20161 atattcacca cggcatgcgt ttcgaagtat cacacagccg agacaaaaaa gacatgtacg
    20221 ttgtgaccga aagtactact aaaccattcg gcaaagacgt taaagaaaaa cgcaccgacg
    20281 tgtatgccgg ttacacctac actcaaccgc tcagtgaagc caccaaactg cgtgccggtt
    20341 taggtttggg ctatgaaaaa tacaaagatg cggtagctaa tgagaaagga acagtcagca
    20401 ccgagcgcga agccttctat accaaagcgc acgccgacct gacttccgat ttgggcggcg
    20461 gctggtatct gaatccttgg gcagaggtta aggttgacct ggacgccaaa ttgaaacaca
    20521 acgcaactgt agccggtgcc tcagccgaca ttaacgcaaa aacacgcgga tggggcgtgg
    20581 gcgtaggtgc gaatatcggc aaacaaatta ccgataccgt cggcatcgaa gccggcccgt
    20641 tctacaaaca ccgtcacttc aaagcatccg gcagcttcgt tttggacggc gggaacatcc
    20701 gagtcgatcc taccaaaatc aacgaatacg gtgttcgtgt cggcgtgaaa ttctaatcgc
    20761 ggcgatatgc cccagacaca aaaccgcaag ccttttggtt tgcggttttt gatgttgtag
    20821 cagaccctaa taccgtctga aacttttcag acggcatttt tccaatcggt taaaataccg
    20881 cctcgacatt gtgttttgga aaacccgtgc ttgcttctgt gattgatttt atcctgcata
    20941 tcgaccaaca cctgctcagc ctgtcggcgc aatacggtgt gtggatttat gcgattctgt
    21001 ttttgattgt tttttgcgaa accggtctga ttgttacgcc cttcctgccc ggcgattcgc
    21061 tcttgtttgc cgccggcggg attgccgcgc tgggcggtat ggatattcat ttgatggttg
    21121 cattattaag ccttgccgcc atactcggcg acgcgctcaa ttttaccgtc ggcaaatatt
    21181 tcggcggcag gctgttcgcc aaccccgatt ctaaaatctt ccggtgcgaa tacctcggca
    21241 aaacccgccg tttttacgaa aaacacggcg gcaaaacgat tatcatcgcg cgcttcgtgc
    21301 ccatcgtccg caccttcgcc cccttcgtcg ccggtatggg aaaaatgcac tatgccaaat
    21361 ttatccgcta caacatcatc ggcggcttgt tgtgggttat cttgttttct tacgcgggct
    21421 atttctttgc caacttcccc gtcgtaaaaa acaatctggg cttggtgatg ggcggcatca
    21481 ttattgtttc cgtcctgccc ggcatcatcg aaatcgcccg cgccaaactt gcggcaaaat
    21541 ccgaacgtta attgttggaa attgacatta tgaatatatt atccgtagaa aacgcttcct
    21601 ttgccgtcgg acatgtcgcc ctgctcgaca aaacgtcctt ccaactcgac agcggcgaaa
    21661 aagtcggctt gatcggacgc aacggcgcag gcaagtcttc gtttttaaaa atccttaccg
    21721 gcgtgcaaaa actcgacgac gggcagatta ttgttcaaaa caatctcaaa atcgtttatg
    21781 tgccgcagga gtcctttttt gataaggacg caaccgtatt tgatactgtt gccgaaggtt
    21841 tgggcgaaat tcgcgattta ttgcgccgtt atcatcatgt cagccatgag ttggaaaatg
    21901 gttcgagtga gctcttattg aaagagctta acgaattgca acttgaaatc gaagcgaagg
    21961 acggttggaa actggatgcg gcagtcaagc agactttggg ggaactcggt ttgccggaaa
    22021 acaaaaaaat cggcaacctt tccggcggtc agaaaaaacg cgtcgcctta gcgcaggctt
    22081 gggtacagaa gcccgatgta ttgttactgg acgaaccgac caaccatttg gatatcgacg
    22141 cgattatttg gttggaaaac ctgctcaaag cgtttgaagg cagcttggtc gtgattaccc
    22201 acgaccgccg ttttttggac aatatcgcca cgcgcattgt cgaactcgat cgcggtattt
    22261 tgcgctccta tcccggctcg ttctccaaat acagcgagaa aaaagcgcaa gagttggcgg
    22321 ttgaagccga acacaaccgc ctcttcgaca aattccacgc gcaggaagag gcgtggatac
    22381 gcaaaggcat cgaagcgcgc cgtacccgca acgaaggccg cgtgcgccgt ttggaagaac
    22441 tgcgccgcca gcgtgccgaa cgccgcaatg tacaaggaca ggtcaacttc aaactcgaca
    22501 gcggcaagaa aagcggcaaa atcatcgccg aactggaaca cgcttcgttt gcctatgacg
    22561 acaaagttat catggataaa ttttccgcca tcttgcagcg cggcgacaaa atcggcttaa
    22621 tcggcccaaa cggtatcggc aaaaccacct tccttaagct gattttgggc gaattgcagc
    22681 cgacctacgg cagaatccgt atcggcagca agcaggaagt cgcctatttc gaccagttcc
    22741 gcagcgcctt gaatgaaaac gatactgtgt tttacacgct cggacagggc aacgattacg
    22801 tcgaagtcgg cggcaagaaa aagcatgtga tgagctattt ggaagatttc ctgttccccc
    22861 ctgcccgcgc gcaaagtcct gtttcttcac tctccggcgg cgaacgcaac cgccttttgc
    22921 tggcaaaact ctttactcgt cccgccaata ttttggtttt ggacgaaccg accaacgact
    22981 tggacatcga cacccaagaa ttgcttgaag atctgttgcg caattaccaa ggcacggtct
    23041 tcctcgtctc gcacgaccgt atgttcctcg acaatgtaat tacccaaagc attgttttcg
    23101 aaggacaagg ccgtctgaaa gaatacatcg gcggctatca ggactatatc gacgcaaaat
    23161 cgcgggaaaa taaaattcag acggcatccg cacccaaagc atccgatgtc gaacccgcca
    23221 aagaaaaacc caaagccaac cgtacggtca aactttccta caaagaacag cgcgaactcg
    23281 acgccctgcc cgacgaaatc gccgccttgg aaaccgaaca ggcagaaatc aacgcgcagc
    23341 tttccgatcc cggaattttc aaagattacg aaaaagcagg agcattgcaa aaccgcgccg
    23401 aagaaatcga aatgctgctt ttggaaaagc tggaacgctg ggagctgctg gaaaccaaac
    23461 agaacggcaa tgccgtctga aatcaaacag gaagaaaaaa aatgttcaaa cgacctgaag
    23521 aaatcatcgt cctgatactt gccgtcctct ggattgccgg tacgtatttt ctcgccgccc
    23581 tgtttggagc ggatgcttat accgtgttaa aaatcactgc ccttaccctg ctttggtctg
    23641 ccgcatcatt cctcctttgg caaaaaaaac ctcagccggc atatctggcc gctgctgctc
    23701 ggctgcctga ctgcctgttg gtggccgtat ctgaatcaat cggtcgcacc cggttcttta
    23761 ccttggcatg catcatggac gttcaaaacc atcttagccc tgattcccgc aaccgcaggt
    23821 tatctgttta aatggaaaca ataccggcag cgcaaaacaa agccgtaagc ccgtcttttt
    23881 tcagacgcca tctccggtca gactgtcaac aattccgata aaatgttata ctcgcgcccg
    23941 ttgccgcgcc gtccgaaacc gccttttggg aaagcagaaa tgatcagcaa acaagaatac
    24001 caagcccaag ccgcccaagg ctacaaccgc atcccgctcg tgcaagaact ccttgccgac
    24061 ttggatacgc cgctttccct ctatctcaaa ctcgccaacc gcccctatac ctacctgctc
    24121 gaatccgttg tcggcggcga acgtttcggc cgctattcct ttatcggcct gccttgcagc
    24181 cactatctca aagccggcgg caaacacgtc gatgtttatc aaaacggcga aatcgtcgaa
    24241 caatacgacg gcaatccgct accctttatc gaagctttcc acaaccgttt caaaacaccc
    24301 gaaatcccaa gcctgccgcg ctttaccggc ggcttggtcg gctacttcgg ttacgaaacc
    24361 gtctacaatt tcgaacactt cgcccaccgc ctgaaaaaca ccgccaaagc caacccgctc
    24421 ggcacgcccg atattttgct gatgctgtcg caagagttgg cggtaatcga caatttgagc
    24481 ggcaaaatcc acctcatcgt ttatgctgat ccctcgcagc ccgacagcta cgaacgcgcc
    24541 cgcgaacgcc tcgaagacat ccgcacccag ttgcgccaaa gctgcgccat cccactttcc
    24601 ctcggcagca aacaaaccca agcagtcagc gagttcggcg aagaaccttt caaagcctgc
    24661 gtcgataaaa tcaaagacta cattttcgca ggcgactgta tgcaggtcgt ccccagccaa
    24721 cgcatgagca tggaatttac cgacaatccg cttgccctct accgcgccct gcgcacgctc
    24781 aacccttcgc cttacctctt ttactacgat ttcggcgatt tccacatcgt cggttcctcg
    24841 cccgaaatcc tcgtccgccg cgaacgcgac gacgtcatcg tccgccccat cgccggcacg
    24901 cgcctgcgcg gcaaaacccc cgccgaagac cttgccaacg aacaagattt gttaagcgac
    24961 gccaaagaaa tcgccgaaca cgttatgctt atcgatttgg ggcgcaacga tgtcggacgc
    25021 atcagcaaaa caggcgaagt caaagtcacc gacaaaatgg tgattgaaaa atactcccat
    25081 gtgatgcaca tcgtttccaa cgtcgaaggc tgtctgaaag agggcgttac caatatggac
    25141 atcctcgccg ccaccttccc tgccggcaca ctctccggcg cgccaaaagt ccgcgcgatg
    25201 gaaatcatcg aagaaatcga accggagaaa cgcggcatct acggcggcgc cgtcggcgta
    25261 tggggcttca acaacgatat ggatttggca atcgccatcc gcaccgccgt gattaaaaac
    25321 aataccctct tcatccaaag cggcgcgggc gtggttgccg actccgaccc aacttccgaa
    25381 tggcaggaaa ctcaaaacaa agcccgcgca gtggttcggg cggcgcagat ggtgcaggaa
    25441 gggttggata aataaatttc agacaacctt cgcagatgaa aagaaggtcg tctgaaaatc
    25501 acaaaacaac atcagagata cttacataaa aaatcacaat gcaattcaca tcattagaaa
    25561 tttgtgccgg agcaggcgga caggctttgg gcttagaaag ggcaggcttt tcccatgttg
    25621 cgttaatcga aatcgaaccg tcggcttgtc aaaccctacg tttaaaccgt cctgattgga
    25681 acgttattga aggagatgtc cgcttgtttc agggcgaagg atatgacggt attgatttat
    25741 tggccggcgg cgtgccttgc cctccttttt ccaaagctgg aaaacaactg ggaaaagatg
    25801 atgaacgcga tttattccct gaggctattc gtctagctaa ggaaactgac cctaaagcca
    25861 tcatgttaga aaatgtgcgg gggttacttg acccaaaatt tgaaaattat cgcaaccata
    25921 ttacagaaca atttgccaag ttgggttacc ttggacaatg gaaactactt tatgctgccg
    25981 attacggagt atcgcaactc agacccaggg ttttatttgt tgcgttgaaa aacgaataca
    26041 ccaatttttt caaatggcct gaaccaaatt cggaacaacc gaaaaccgtt ggagaactgc
    26101 tttttgactt aatgtcagaa aataactggc agggggcgca taactggcgt ttgaaggccg
    26161 cgcagattgc accgaccttg gtcggcggtt caaaaaaaca cggcggtgct gacttggggc
    26221 ctacacgatc caaacgcgca tgggcggagt tgggtgtgga tggttcaggt ttgtgggata
    26281 gtgcgccgcc tgaagacttt tcgggaatgc ctagattgac tgtccgaatg acggcgcgta
    26341 tccaaggatt tcctgataac tggcaatttt ttggtaaaaa aacgcctatg taccgccaaa
    26401 tcggcaacgc ttttcccccg ccggtagccg aagcggtggg caggcagatt atcaaagcct
    26461 taaaaaagga aaattgaatg aatccgcttt ttacccaaga acgcaggatt ttccataaga
    26521 aattattgga tggcaatatt cttgcaacca acaatagagg cgtagtcagc aatgcggatg
    26581 ggagcaatac ccgaagtttc aatatagcca aggggattgc cgacttactg cattcggaaa
    26641 cggtttcgga aagattaccc ggtcagacat cgggaaatgc ttttgaagca atatgcagtg
    26701 agtttgtcca atctgctttt gagaaattgc agcatatcag acccggcgac tggaatgtta
    26761 agcaggtcgg ttctcgcaac cgtctggaaa tcgcacgtta tcagcaatac gcacatctga
    26821 cagccttagc caaggctgcc gaagaaaacc ccgaattggc tgccgccttg ggcagtgatt
    26881 acaccattac tcccgatatt atcgttacca gaaatttgat tgcagatgca gaaatcaacc
    26941 gtaatgaatt tttagttgat gaaaatattg ccacatatgc cagtttgcgt gcgggtaatg
    27001 gcaatatgcc gctgctccac gccagtatct cttgtaaatg gacgattcgc agcgacaggg
    27061 ctcaaaatgc ccgttctgaa ggattgaatt tggttagaaa cagaaaaggc agactgccgc
    27121 acattgttgt cgtaacggca gaaccaacgc caagccgcat ttcatctatt gccttgggta
    27181 caggggaaat agattgcgtt taccattttg ccttatatga gctggaacaa attttacaat
    27241 cattgaatta tgaagacgct ttggatttgt tttacatcat ggtcaatggt aaacgattaa
    27301 aagatatttc cgaccttccc ttagatttgg cagtttaaac agaattattc cactatgaac
    27361 acacccccca tccttcctcc cgccatgctc ggcatcctcg gcggcggaca attaggcaga
    27421 atgtttgccg ttgccgctaa aaccatgggc tacaaagtaa ccgttctcga tcccgacccg
    27481 aatgcgccgg cggcggaatt tgccgaccgc catttgtgcg cgccgtttga cgaccgggcc
    27541 gcgttggacg aattggcaaa atgcgcggcg gttacgaccg aatttgaaaa cgtcaatgcc
    27601 gacgcgatgc gctctctggc aaagcatacc aacgtttccc ccagcggcga ctgcgtgtcc
    27661 attgcacaaa accgcattca ggaaaaagcg cggatacgca aagcaggctt gcaaaccgcg
    27721 ccgtatcagg cggtttgcaa ggccgaagac attactgaag caagcgcgca atttttgccc
    27781 ggcatcctga aaacggctac gttgggctac gacggcaaag gtcaaatccg cgtcaaaacg
    27841 ttggacgaac tcaaagccgc gtttgccgaa cacggcggcg tggattgcgt tttggaaaaa
    27901 atggtggact tgcgcggcga gatttccgtg atcgtatgcc gtctgaacga tgaaaacgtg
    27961 caaaccttcg accccgccga aaacatccac gaaaacggca tcttggctta ttccatcgtc
    28021 cccgcgcggc tgagtgccga cgtgcagcaa caggcgcggc agacggcgca acgcttggcg
    28081 gacgaattgg attatgtcgg cgtattggcg gtagaaatgt ttgttgtcgg cgacacacat
    28141 gaattgctcg tcaatgaaac cgccccgcgc acgcacaatt ccggccacca tacgatagat
    28201 gcctgcgccg cagaccagtt ccaacagcag gtacgcatta tgtgcaacct gccgcccgcc
    28261 gacaccaaat tattatcccc ttgctgtatg gcgaatattt tgggcgacgt ttggcaggaa
    28321 gatggcggcg aaccggattg gctgccgttg caaagccggc cgaatgcaca cctgcaccta
    28381 tacggaaaaa aaaccgcaca gaaaggtcgg aaaatgggac actttaccgt tttgaccacc
    28441 gattcggaca ccgcatttca agaagcaaaa aaactgcatc agtccctata atccgaccaa
    28501 cggcacagat gccgtctgaa gccgccgaca taccgccatg agcctgctga cactgttacg
    28561 ccccgccacc gtgcaagact gcaaagacat cttcaaagtc cacctgcact ccgtccaata
    28621 cacctgcatc ctcagctaca acgaacacgc cctaaaagta tgggaaggct tgttaaacac
    28681 agaaagctac ctgccgacca tttccgatcc cgacaaagca ctttgggttg ccgaatacaa
    28741 aggcaacata cagggatttt tccaaatcga ctgccaagaa gcacaactcg atgcgcttta
    28801 cgtccacccc ctgttccaca atctcggact gggcactgcc ctgctccgtc aggcagaaac
    28861 catcgcccac aaatccggtt tgagctttct gaaactctac gcatctctca actccgtccc
    28921 gttttacctt ctcaaccgct acgaatcgct cggttcggca gtattgcagc ttgaccccag
    28981 catcaaaatc aaatgcgaac tgatgcgcaa acacctctaa tcccgcctta taaccaaaac
    29041 tgataatctt caaatcaaac attatttcca caagcgcaga cagccgtata aagttgcccc
    29101 atctaccaac cgacaggaca agcatatgaa aacctacgca caggcactct ataccgcagc
    29161 cctgctcacc gcctgcagcc ccgcagccga ttcaaaccat ccgtccggac aaaatgcccc
    29221 ggccaatacc gaatccgacg gaaaaaacat taccctgctc aatgcctcat acgatgtgac
    29281 acggtatttt tacaaagaat acaaccactt atttatcaaa acataccaat ccgaacaccc
    29341 cggcacatcc gtcagcatcc aacaatccca cggcggcttc agcaaacagg cattatccgt
    29401 agccaacggc cttcaagccg atgtcgtaac catgaaccaa tcttccgaca tcgacctgct
    29461 cgaaaaaaag gactggtaga aaaaggctgg caacaagccc tccccgatca cgccgcaccc
    29521 tacaccagca ctatggtttt ccttgtccga aaaaacaacc ccaaacagat ccgcgattgg
    29581 aacgaccttg ccaaagacgg cgctaacatc gtcatcgcca agacctcggg caacggacgc
    29641 tacgccttcc tcggcgcata cggttacggt ctgaaagcca acaacggcaa cgagcaggaa
    29701 gcccaaaaac tcgtcgcatc catcctcaaa aacacacccg tttttgaaaa cggcggacgc
    29761 gccgccgcca ccaccttcac acaacgcaac atcggcgacg tactcatcac ttttgaaaac
    29821 gaagccaact acgtcagcaa aaaactgacc caaggacagt ttgaaatcgt ctatccgagc
    29881 tacaccattt ccgccgaaag ccccgttgcc gtcgtcaaca gcgtcgtcgc gaaaaaaggc
    29941 acgcaaaaaa ccgcccgcgc ctatctcgaa tacctttgga gcgaaccggc gcaagaactc
    30001 gccgcctcac tctacctgcg tccccgcaac cccgaagtat tggcacgaca caaagccgac
    30061 ttccccgact tagacacctt ccctcccgaa gaaaaattcg gcggatggga caacatcatg
    30121 aaaacctact tcgccgacgg cggcgtattc gaccgcctga ccgcacaaaa atgaccatta
    30181 tgccgtctga accgcctttc agacggcatt tcacacatca aaacagaaaa ccgcacccaa
    30241 aatcatcatc acaagacaca acggcaaacc ggatacaaaa cccaacaccg ccgccaaaat
    30301 gccgtctgaa gccgtcggcg caaccatcct tatctggggc gcgcctcatc aaaggggcgc
    30361 ataactttcc ttaaccatcc cttttgacag ccaaataaac agtgcttttg cctgtcagaa
    30421 acaaaaccaa tattttgcgg ttcgtatcgg aacatttata aaacatcaga tttccgtgtt
    30481 tcgcgtttcc ggcaattcaa gattagcaaa accattaacc tgcttcgcaa ggcaggtttt
    30541 taaaacttta tcaggcgttc gagcatatcg gttcgaccga cccgcatttc cggcaatcaa
    30601 cactgccgac ggttgccgca tttctttttg atatacaatc acactttcaa aaaacatacg
    30661 acggcaacaa aatcatcctc cccccgcttg gcaaacatcc ggcaaattaa aaccccgccc
    30721 gaaacagcca tcggacaaat aacaggaaaa aatatgaata ccgacttcca aaagatcctc
    30781 aaacaactgg gcaaacaggc caaaaaagaa gcggcagaaa aacaggaagc cgaaaaaaac
    30841 aagcaaaaac aagaatatga tttggatttt tcacaagcag tcggacaagt ctccccatta
    30901 aaaaaccggc agcaatatta tgcgcagtcc gacaaaaccc ccatcaaagc ccgtcccaaa
    30961 gacaaccgca ccgacgaaga aaactacttc tacataggca gcacatacaa cgacccaccc
    31021 gccagcttct gcaaaaacgg acagggaaaa aacgacatcc aacgcctgaa aaacggatac
    31081 tatcccgtcg ttaccgatgt cgacctgcac ggctacaccc aagaagaagc acaaaaagtc
    31141 ctcaacgaat tcatcgcatt cacccaaaaa cgcggcgtat gcggcgaaat catccacggc
    31201 agcggattag gttccaaagg ctacaaaccc gttctgaaaa acatgacccg aaactggctg
    31261 atgcaacacc ccgacgtact cgcctacgtc gaaccccggg aaggcaacga cggctgcgtc
    31321 cgcatcctgc tcaaacgcaa attaaggcag caagactgac aaatttctcc gacagacttg
    31381 gcagcaccgc acaatatccg tataatttgc aaaccatttc tgggggcgac cttggtttcg
    31441 acgggggttg cgaagcagat gcgggcatac cggggtctca gattcccgta aaacactgaa
    31501 ttcaaatagt cgcaaacgac gaaacttacg ctttagccgc ttaaggctag ccgttgcagc
    31561 agtcggtcaa tgggctgtgt ggcgaaagcc accgcaacgt catcttacat tgactggttt
    31621 ccagccgggt tacttggcag gaaataagac ttaaggtaac tggtttccaa aaggcctgtt
    31681 ggtcggcatg atggaaataa gattttcaaa tagacacaac taagtatgta gaacgctttg
    31741 tagaggactt tcggacgggg gttcgattcc ccccgcctcc accatgccct attttcagca
    31801 gtatcctgca acacaaaaaa tccttgaact tcaaaagttt caaggctttt ttattgcctt
    31861 gaatatgatg gcgtatgctt gagttttcgc aatttatggg gtaacttttg gggtaacttt
    31921 tcaggaatca ccctaaaaaa tatcttaaaa atacaaaagc catcacgcat tttttaaaaa
    31981 ggttacccaa aacttaaatg aagcctttat ttataaggct tgcagctttc caaacaagtt
    32041 acccccaaac ggataaaaaa tgccgaag
//
LOCUS       DAXDSD010000013        30512 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXDSD010000013 DAXDSD010000000
VERSION     DAXDSD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30512
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.013"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..228)
                     /locus_tag="ACFB2Q_000445"
     CDS             complement(<1..228)
                     /locus_tag="ACFB2Q_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC0917548.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRAL"
     gene            complement(298..1164)
                     /locus_tag="ACFB2Q_000446"
     CDS             complement(298..1164)
                     /locus_tag="ACFB2Q_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC0917549.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(1213..1491)
                     /locus_tag="ACFB2Q_000447"
     CDS             complement(1213..1491)
                     /locus_tag="ACFB2Q_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC0917550.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(1491..1781)
                     /locus_tag="ACFB2Q_000448"
     CDS             complement(1491..1781)
                     /locus_tag="ACFB2Q_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC0917551.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(1784..2314)
                     /locus_tag="ACFB2Q_000449"
     CDS             complement(1784..2314)
                     /locus_tag="ACFB2Q_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC0917552.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLFF
                     FIIQGIYLSTYLKKED"
     gene            2622..4938
                     /gene="tspA"
                     /locus_tag="ACFB2Q_000450"
                     /pseudo
     CDS             2622..4938
                     /gene="tspA"
                     /locus_tag="ACFB2Q_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
     gene            complement(5111..5527)
                     /gene="gloA"
                     /locus_tag="ACFB2Q_000451"
     CDS             complement(5111..5527)
                     /gene="gloA"
                     /locus_tag="ACFB2Q_000451"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC0917553.1"
                     /translation="MRLLHTMLRVGNLEKSLDSYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGDAYGHIAVEVDDAYEACERVKRQGGNVVREAGL
                     MKHGTTVIAFVEDPDGCKIEFVQKKSGDDSVAYANT"
     gene            complement(5552..6721)
                     /gene="lapB"
                     /locus_tag="ACFB2Q_000452"
     CDS             complement(5552..6721)
                     /gene="lapB"
                     /locus_tag="ACFB2Q_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC0917554.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(6732..7046)
                     /locus_tag="ACFB2Q_000453"
     CDS             complement(6732..7046)
                     /locus_tag="ACFB2Q_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC0917555.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSVNLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            7356..8354
                     /gene="ilvE"
                     /locus_tag="ACFB2Q_000454"
     CDS             7356..8354
                     /gene="ilvE"
                     /locus_tag="ACFB2Q_000454"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC0917556.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(8599..9384)
                     /gene="fabI"
                     /locus_tag="ACFB2Q_000455"
     CDS             complement(8599..9384)
                     /gene="fabI"
                     /locus_tag="ACFB2Q_000455"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC0917557.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(9539..10360)
                     /gene="dapD"
                     /locus_tag="ACFB2Q_000456"
     CDS             complement(9539..10360)
                     /gene="dapD"
                     /locus_tag="ACFB2Q_000456"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC0917558.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(10570..12213)
                     /gene="pgi"
                     /locus_tag="ACFB2Q_000457"
     CDS             complement(10570..12213)
                     /gene="pgi"
                     /locus_tag="ACFB2Q_000457"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC0917559.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPCNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            12723..13955
                     /gene="pilG"
                     /locus_tag="ACFB2Q_000458"
     CDS             12723..13955
                     /gene="pilG"
                     /locus_tag="ACFB2Q_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC0917560.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            14029..14889
                     /locus_tag="ACFB2Q_000459"
     CDS             14029..14889
                     /locus_tag="ACFB2Q_000459"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC0917561.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            14886..15518
                     /gene="coaE"
                     /locus_tag="ACFB2Q_000460"
     CDS             14886..15518
                     /gene="coaE"
                     /locus_tag="ACFB2Q_000460"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC0917562.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            15511..15720
                     /locus_tag="ACFB2Q_000461"
     CDS             15511..15720
                     /locus_tag="ACFB2Q_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC0917563.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(15806..17482)
                     /gene="pilB"
                     /locus_tag="ACFB2Q_000462"
     CDS             complement(15806..17482)
                     /gene="pilB"
                     /locus_tag="ACFB2Q_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC0917564.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(17728..17799)
                     /locus_tag="ACFB2Q_000463"
     tRNA            complement(17728..17799)
                     /locus_tag="ACFB2Q_000463"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17764..17766),aa:Arg,seq:cct)
     gene            complement(17820..18266)
                     /locus_tag="ACFB2Q_000464"
     CDS             complement(17820..18266)
                     /locus_tag="ACFB2Q_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC0917565.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(18270..19244)
                     /locus_tag="ACFB2Q_000465"
     CDS             complement(18270..19244)
                     /locus_tag="ACFB2Q_000465"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC0917566.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIRVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMIQLAKESLVRVS"
     gene            19468..19776
                     /gene="rplU"
                     /locus_tag="ACFB2Q_000466"
     CDS             19468..19776
                     /gene="rplU"
                     /locus_tag="ACFB2Q_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC0917567.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            19801..20073
                     /gene="rpmA"
                     /locus_tag="ACFB2Q_000467"
     CDS             19801..20073
                     /gene="rpmA"
                     /locus_tag="ACFB2Q_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC0917568.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            20308..21492
                     /gene="ubiM"
                     /locus_tag="ACFB2Q_000468"
     CDS             20308..21492
                     /gene="ubiM"
                     /locus_tag="ACFB2Q_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC0917569.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEIVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(21681..21836)
                     /gene="rpmG"
                     /locus_tag="ACFB2Q_000469"
     CDS             complement(21681..21836)
                     /gene="rpmG"
                     /locus_tag="ACFB2Q_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC0917570.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(21868..22101)
                     /gene="rpmB"
                     /locus_tag="ACFB2Q_000470"
     CDS             complement(21868..22101)
                     /gene="rpmB"
                     /locus_tag="ACFB2Q_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC0917571.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(22351..23874)
                     /gene="farB"
                     /locus_tag="ACFB2Q_000471"
     CDS             complement(22351..23874)
                     /gene="farB"
                     /locus_tag="ACFB2Q_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC0917572.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(23898..25052)
                     /gene="farA"
                     /locus_tag="ACFB2Q_000472"
     CDS             complement(23898..25052)
                     /gene="farA"
                     /locus_tag="ACFB2Q_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC0917573.1"
                     /translation="METHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGGVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGDVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      25293..25337
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            25341..25814
                     /gene="queF"
                     /locus_tag="ACFB2Q_000473"
     CDS             25341..25814
                     /gene="queF"
                     /locus_tag="ACFB2Q_000473"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC0917574.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            25889..26575
                     /locus_tag="ACFB2Q_000474"
     CDS             25889..26575
                     /locus_tag="ACFB2Q_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC0917575.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPAASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCILFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(26925..28217)
                     /locus_tag="ACFB2Q_000475"
     CDS             complement(26925..28217)
                     /locus_tag="ACFB2Q_000475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC0917576.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGGTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            28294..29262
                     /locus_tag="ACFB2Q_000476"
     CDS             28294..29262
                     /locus_tag="ACFB2Q_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917577.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLHAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADNQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            complement(29463..>30512)
                     /locus_tag="ACFB2Q_000477"
     CDS             complement(29463..>30512)
                     /locus_tag="ACFB2Q_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC0917578.1"
                     /translation="HYRELIAAQPDAPAVRMRLAAALFEDRQNEAAADQFDRLKTEDL
                     PPQLMEQVELYRKALRERDAWKVNGGFSITREHNINQAPKQQQYGNWTFPKQVDGTAV
                     NYRFGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKFNDMTAGVSGGIGFADRRKDVGLA
                     VFHERRTYGNDAYSYANGARLYFNRWQTPRWQTLSSAEWGRLKNTRRARSDNTHLQIS
                     NSLVFYRNARQYWTGGLDFYRERNPADRGDNFNRYGLRFAWGQEWGGSGLSSLFRLGV
                     AKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRALHFKGITPRLTLSHRETWSNDVFN
                     EYEKNRAFVEFNKTF"
BASE COUNT         7282 a         8811 c         7627 g         6792 t
ORIGIN      
        1 caatgccctg tttttcaaaa cttccaaagt ttgcagccgg cggacggcat cgtttcggat
       61 tgcctgcccg tccggtcttt gggactgctc cgcatgccgg tctgcctgct gcatgatctg
      121 cgccaccagc gtatccacca aagccgggtc gatttcgggt gctttggctg ccgcaaaacc
      181 tgccaacatt gcagcaataa ctgcggcagc ggtctttttc tgtttcatgg tattccctgt
      241 cctgcgcggc ggcaaaacgc ctgtatgaac gtcttgccgc cgtatcccgt tttcagatta
      301 ttttgcaggt ttgatgtttg ccttacccaa cagcgcaccg acggcacggt caatccgttc
      361 cgcctgaagg ttgccggcaa tctgtccttt catttcgtca aaagaaggca ctttcacctc
      421 gcggctgtcg ttgacataat aaacgccgta gaaatcgccg tttttcagcg gcgttgccgt
      481 aaattcgcct tttttcaagt ccttaattgc ctgataaagc ggcggaacac cctgttccaa
      541 atctttcagc ggcacatatc cgtccggcgc gccggtccgt ttggtgcggt cgttgagcga
      601 gtattgtttc aaaacggcat caaaaccttt tttcgccttc aaatcggcaa ccgctttttt
      661 cgcattttct tccttgtcgg tcaggatttc gcccaactgg acttcctgcg tgcctttata
      721 aaaaccgctg atattgtcgt aaacggcttt tacttcctgc tcggaaaccg gttgggtttt
      781 ggcgatatgc agtgcgtatg cctcgccgtt caagccatat tttaccgcct gccaaacggt
      841 tttgaaggac ggtttcttgt cgtcgcccga ctttttcgct tcggcacgca atttggcaag
      901 cgcatcttta aactctgccg accggtcgag tttcaggcgt ttcacttcct gtgcgaccac
      961 ggtgttgacc acttcgtttt ccagcaggga ttggcgcagt tgcggcgtgt cttcggcacg
     1021 gctgttttcc gcacggaatg cggcaacctg cgcatcgatg acggaactgt cgattttctg
     1081 accgttaacg gttgccagcg tttgggcaaa caggctgccg gaacaggcaa gcagcgcaac
     1141 ggaagtcagg atttttgctt tcataatctt ctctctttaa attttaaatt gaaatattca
     1201 ggcagacttc attcagtccg ccgtatggaa atactcgtca ggggtagccg cccggatgcc
     1261 gagcgcgtga atcatgccgc ctgaaaataa atctttgagc agcgatttga ccgttttctg
     1321 acggttcaga cggcttaaac cttcaaaacg gccgctgacg accaaaacgg cataatgtcc
     1381 gccgccggta ttgcccgcgt gtcctttgtg cagatggctc tcatcgccga tttccaacac
     1441 cagaggatcg agcgtctgca ggcgttcgcg gatcaaatcg acagccggca ttatttgaac
     1501 accgctttaa acggcttgat cagcacttcg ctgtacacgc cggcatgaac atagggatcg
     1561 tcttcagccc aagcctgcgc cgcatccaaa gactcgaact gtgccacaat caagctgccc
     1621 gaaacacgtt cgggattgtc cggcagcagg tttgggcctg ccgtcagcag acggccttcc
     1681 gatttcagcg tttccagccg tttaaagtgt tcgggacgtg ccgccatacg cgcttcatgc
     1741 acatcctccc cgtctgttgc cagcaacata aaatattcca cagtcaatcc tcctttttca
     1801 gataggtact cagataaata ccctgaataa taaagaaaaa aagcatcagc gcagtcgaac
     1861 cgaacatctt atagttaacc cattgcgctt caaacctagt aaacacaaac cagttggcaa
     1921 tacccataaa aatcagaaaa ccgacccaca tatatgtcaa ttttccccat acggcatccg
     1981 gaagctgaat ctccctgccg atactcgctt tcaagccgtt tttacccgca aggtggctgc
     2041 ccagcaggaa taacgccccg caccagaaca atactgtcgg cttccacata atgaagcggc
     2101 tgtcgcccaa aacaatggtt gcgccgccga atacgacaat cagcaccagt ccgacccact
     2161 gcatcgtatc cagcctttta tgcttccaat acaggaaagc cgcctgaacc acgcctgcaa
     2221 ccaaggcaac cgccgcagcg gcaatcatat ttttggtaac ggtataagta gcaaaaaaca
     2281 agatgacgga caaaaggtcg ctgacaaatt tcatatccaa atgagaaagc aaatcaaata
     2341 aacgctaatc atacaggcag aaccctatct ttgcacctgc aaaaccaaca aattttgttt
     2401 tgattaatgc aaatatgcac agatgttttt gaaaaaagat ggaaatatgt cataatttat
     2461 aaaattggct ttaaaataca gcttcggtcg gaacctctgc ctatccgcac cctatcgtcc
     2521 gtattgccta tgcctgatga cgaaatttac agactgtacg cggtcaaacc gtattcagcc
     2581 gccaacccac aggggataca tcttgcaaaa acaacagaca aatcaaactg attgccgcct
     2641 ccatcgcagt tgccgcatcc tttcaggcac atgcgcgact gggcggactg aatatccggt
     2701 ccaaccttga cgaacccttt tccggcagca ttaccgtaac cggcgaagaa gccaaagccc
     2761 tgctaggcgg cggcagcgtt accgtttccg aaaaaggcct gaccgccaaa gtccacaagt
     2821 tggacgataa agccgtcatt gccgtttctt ccgcacaggc agtccgcgat cccgtcttgg
     2881 tgttccgcat cggtgcaggc gcgcaggtac gcgaatacac cgccatcctc gatcctgtcg
     2941 gctgctcgcc caaaaccaaa tctgcacttt cagacggcaa gacacaccgc aaaaccgctc
     3001 cgaaagcaga gtcccaagaa aatcaaaacg ccaaagccct ccgcaaaacc gataaaaaag
     3061 acagcgcaaa ctcagccgtc aaaccggcgc ataacggcaa aacccatacc gtccgcaaag
     3121 gcgaaacgct caaacagatt gccgccgcca tccgtccgaa acacctgacg ctcgaacagg
     3181 ttgccgatgc gctgctgaag gcaaacccaa atgtttccgc acacggcaga ctgcgtgcgg
     3241 gcagcgtgct tcacattccg aatctgaaca ggatcaaagc ggcagcaccc aaaaaaatca
     3301 aagcggaaca acccaaaccg caaacggcga aacccaaagc cgaaactgca tccatgccgt
     3361 ctgaaccgtc caaacaggca acggtagaga aaccgataga gaaacctgtc gaaaaaccgg
     3421 ttgaaaaacc tgaagcaaaa gttgccgcgc ccgaagcaaa agcggaaaaa ccggccgttc
     3481 gacccgaacc caaacccgca gtgtccgaaa ctccggcttc ggcgacagaa cgccaacccg
     3541 gacctgtacc cgctgcaaat actgccgcat cggaaaccgc tgccgaatcc gcccccccaa
     3601 gaagccgccg cttctgccat cgacacgccg accgacgaaa ccggtaacac cgtttccgaa
     3661 cctgttgcgg aaacagccag tgaagttgcc gccgaacccg ttgccgaacc tgtcgaacag
     3721 gtttctgcca aagaagaaac cgaaagcgga ctgtttgacg gtctgttcgg cggttcgtac
     3781 accttgctgc ttgccggcgg aggcacggca ttgatcgccc tgctgctgct tttacgcctt
     3841 gcccaatcca aacgcgcgcg ccgtaccgaa gaatccgtcc ctgaagaaga gcctgacctt
     3901 gacgacgcgg cagacgacgg cataaaaatc acctttgccg aagtcgaaac tccggcaacg
     3961 cccgaacccg ctccgaaaaa cgatgtaaac gacacacttg ccttaggtgg ggaatctgaa
     4021 gaagagttat cggcaaaaca aacgttcgat gtcgaaaccg atacgccttc caaccgcatc
     4081 gacttggatt tcgacagcct ggcagccgcg caaaacggca ttttgtccgg cgcacttacg
     4141 caggatgaag aaacccaaaa acgcgcggat gccgattgga acgccatcga atccacagac
     4201 agcgtgtacg agcctgagac cttcaacccg tacaaccctg tcgaaatcgt catcgacacg
     4261 cccgaaccgg aatctgtcgc ccaaactgcc gaaaacaaac cggaaaccgt cgataccgat
     4321 ttctacaaca acctgttctc aaacaaccat atcggcacag aagaaacagc ttccgcaaaa
     4381 cctgccgcac cctccggact ggcaggcttc ctgaaggctt cctcgcccga aaccatcttg
     4441 gaaaaaacag ttgccgaagt ccaaacaccg gaagagttgc acgatttcct gaaagtgtac
     4501 gaaaccggtg ccgtcgcgga aactgcgcct gaaacgcccg atttcaacgc cgccgcagac
     4561 gatttgtccg cattgcttca acctgccgaa gcaccggccg ttgaggaaaa tgcagcggaa
     4621 atcactttgg aaacgcctga ttccaacacc tctgaggcag acgctttgcc cgacttcctg
     4681 aaagacggcg aggaggaaac ggtagattgg agcatctacc tctcggaaga aaatatccca
     4741 aataatgcag ataccggttt cccttcggaa tctgtaggtt ctgacgcgcc ttccgaagcg
     4801 aaatacgacc ttgccgaaat gcatctcgaa atcggcgacc gcgatgccgc tgccgagaca
     4861 gtgcagaaat tgctggaaga agcggaaggc gacgtactca aacgtgccca agcattggcg
     4921 caggaattgg gtatttgatt cccaactgcc ctttcgcaga tcaaggatgc cgtttcagac
     4981 ggcatctttt tttgccttat cggtgtaacg gataaagttt gaaccggcac aggctcaaac
     5041 agcaggccga cggcaacaaa atgccgtccg aaacccctaa aggcttcaga cggcattggc
     5101 ggcggcggta tcaggtattc gcataggcaa ccgaatcgtc gccgcttttc ttttgaacga
     5161 actcgatttt gcatccgtcg gggtcttcta cgaaggctat cacggttgtg ccgtgtttca
     5221 tcaggccggc ttcgcggacg acgtttccgc cctgccgctt cacgcgttcg caggcttcgt
     5281 aggcatcgtc cacttcaacc gcgatgtgtc cgtaggcgtc gcccaagtcg tatcgttccg
     5341 tatcccagtt gtgcgtcagt tccaaaaccg tgctgtcggt ttcgtcgccg taaccgacga
     5401 aggcaagggt aaatctgcct tcgggatagt cttttcggcg gagcagtttc atacccaaaa
     5461 cgttttggta ggaatcgagg gatttttcga gattgcccac gcggagcata gtatggagta
     5521 agcgcatttt tcgtgttcct ctcggtggtg gttaaacttc gattttattc ggcgtaaacg
     5581 tctgccattt gttgcaggcg ggacagtgcc agaaaaagac ttgggatttg aagtggcagt
     5641 tgcggcaacg gtacatcacg ctgcgctgga gctgccgtcc gataaccgaa cgcatcatgt
     5701 cggcatcggc tttccaagcc ggatccaaat cgctgagttt caaaccgagc aggcggtaca
     5761 cgccgttaag gtcgggcttg cggcggacaa gctcgacggc ggtttgcgcg gcttctttct
     5821 cgcccttaag cagcagggat ttctcgtaca cgacattgat caggtcaagt tcgggaaacg
     5881 tctgcatata tcctgtcaga cggttcaagc cttcttcagg ttttccctgc gcggcatagg
     5941 cttcgtaaag cttctcgccg accatgctca agtatgcatg gttttgctgc tcgatggcgg
     6001 cataggcttc gacggcggca gggaaattgc cttgtcggtg ttcaatgtcg cccaaaatca
     6061 tgttggcgcg ggtgcatttt ttgttggctt cgagtgcctt gccgacattg aaacgcgcgg
     6121 catcgaaatt ggacttgaac agcgcggctt gggcaagttc gcaataaaac tgtgcaatct
     6181 caaactgata tgtctgttcg tcgtgactaa gaagttgggc ggtttcaacc gctttttccc
     6241 aatccctgtc ctgctggtag atattgagca ggtgctgtct ggcttcacgc gccatttcac
     6301 cgtcttgcag ccccaaaaaa atctgttcgg cacgatcgac caaacccgcg ctttggtagt
     6361 tttgcgccaa ttcaaacagg acgcgcgcgc gcttttcgcc gaccgtatcg ggagaatcga
     6421 gcattgtccg gtgtatgttg atggctttgt cgttttcgcc gcgctgacgg taaagtttgc
     6481 cgagggtaag gttcaaatca tacgattgcg gccggccgtc gacgacttcc gccaactccc
     6541 ttgccgcgcg cccgctgttg cggtcgacca aagcgtccag gcttttataa aatcccgaag
     6601 ggatgctttt tgcctgcttc aatacggttt tcatatccac gcgggcggca aaccagccca
     6661 tcgtgaagaa gacgggcaaa aggataatcg gcagcaggat aatccacaat tcgttgtcca
     6721 tatcggcttt cttaaggctg tttggcagat tcggcagcat tttgtatcgg cggtgcagtc
     6781 aatttctgtc cgctcaagcg cgcacttttc ttcacttccg cacgcaggcg gctgttttcg
     6841 ccgcgcaagg acagcagccg cccgaacagg gcaaacattc cgaacacgat gccgacgaca
     6901 aacgcgccga acaatacgac aatcagcggc agattgacac tctgccccgg aagataggaa
     6961 aaggtaacgg catccatatt aatgacggca agcagcagga agagcagcag gataatgatt
     7021 ttgatgacgg tatagataag tttcatgttt gctccgtaaa taaaaacgcc gcacggacag
     7081 cttcgcactc tgaaccaaga ttgcgttagt ttaaacgatt tgcacggttt cggatagaat
     7141 gcggcagcgt gttcggacgg catacggagt atgcgattgc cgacaatttt accaaataca
     7201 ccgctccttt ccatttgaaa aataacggat tggacaccgc atcgacagaa aaacccgccg
     7261 cgcacttgtc aaaaccctgt ttgcaggcgt atctttacaa ccttcaaatt caaaccgttc
     7321 attgaaacat atcagaataa gaaaggcttt acatcatgag cagacccgta cccgccgtat
     7381 tcggcagcgt ttttcacagt caaatgcccg tcctcgccta ccgcgaaggc aaatggcagc
     7441 cgaccgaatg gcaatcttcc caagacctta cccttgcacc cggcgcgcat gccctgcatt
     7501 acggcagcga atgctttgaa ggtttgaagg ccttccgtca ggcagacggt aaaatcgtgc
     7561 tgttccgtcc gaccgccaat atcgcgcgta tgcggcaaag tgcggacatt ttgcacctgc
     7621 cgcgccccga aacccaagct tatctcgatg ctttggtcga actggtcaaa cgtgccgccg
     7681 acgaaattcc cgatgcgcct gccgcgctgt acctgcgccc gaccttaatc ggtaccgatc
     7741 ccgttatcgg caaggccggt tctccttccg aaaccgccct gctgtatatt ttggcttccc
     7801 ccgtcggcga ctatttcaaa gtcggctcgc ccgtgaaaat cttggtggaa accgaacaca
     7861 tccgctgcgc cccgcatatg ggccgcgtca aatgcggcgg caactacgct tccgccatgc
     7921 actggttgct gaaggcgaaa gccgaatacg gcgcaaatca agtcctgttc tgcccgaacg
     7981 gcgacgttca ggaaaccggc gcatccaact ttatcctgat taacggcgat gaaatcatta
     8041 ccaaaccgtt gacagacgaa ttcctgcacg gcgttacccg cgattccgtg ctgactgtcg
     8101 ccaaagattt gggctatacc gtcagcgaac gtaatttcac ggttgacgaa ctgaaagctg
     8161 ctgtggaaaa cggtgcggaa gcgattttga ccggtacggc cgccgtcatc tcgcccgtta
     8221 cttccttcgt catcggcggc aaagaaatcg aagtgaaaaa ccaagaacgc ggctatgcca
     8281 tccgtaaggc gattaccgac atccagtacg gtttggcgga agacaaatac ggctggctgg
     8341 tcgaagtgtg ctgatgcttt aaataaaaaa atgccgtctg aaacccgttt ggcgtttcgg
     8401 acggcatttt cgcgttcgga ccgtttccgc cgcacctgca gcaagtcggt acaaaggcaa
     8461 tcggttaaaa caagcgtccg catttcccat cccgcctgcc gcaagtcggg catttccctg
     8521 gaaatacgct tcaaacggca aaccgccgcc cgaaaccgat actcggcacg gacggcgcac
     8581 gaatttgaaa acggcggatt atccctcggt gctcaaggca ttgatgctgt aaccgccgtc
     8641 aacataagtg atttcgccgg taatgccgga cgacaggtcg gacagcagga aggcggcagt
     8701 attcccgact tcttcgatgg taacgttgcg gcgcagcggg ttgtgggcgg cgacgtgtcc
     8761 caagagtttg ccgaaatcgg cgatgccgga ggcggcgagc gttttgatcg ggccggcgga
     8821 aatgccgttg cagcggatgc cctctttacc cagacaggcg gcggtaaagc ggatgcctgc
     8881 ctcaaggctg gcttttgcca tacccatcac gttgtaattc ggaatcgcgc gtaccgcgcc
     8941 caagtagcta agggcgacga tggcggagtt tctgccgcgc atcatcggac gggcggcttt
     9001 tgccaatgcg ggcaggctgt atgcggaaat ttcgtgtgcg gtgttgaacg cttcgcggct
     9061 gatgctgtcg aggaagtcgc cgctcaaggc ttctttcggc gcgaagccga tggaatgcac
     9121 caaaccgtcc aagccatccc aatgtttgcc taagtcggcg aacacttggt tgatttcgtc
     9181 gtcgctggcg acatcgcagc ggaatacgag ttcggaatct aattccgccg ccattttgcg
     9241 gacgcgctct tccagtttgt ccacaacgta ggtaaacgcc agttccgcgc cctgttcgcg
     9301 gcaggctttg gcgatgccgt aagcgatgga acgctcggaa atcatgccgg taatcagaat
     9361 ttttttgcct tgcagaaaac ccattttgta tccttcaaat agtgttcggt tcgttctaaa
     9421 ccgttgatta tagcaaattg tccctgtttc tgtgttttca cgttgcagcg tgcggacggc
     9481 aatgccgtcc gaagcggatt tcagacggca ttggacgttt caaatacggt ttaaggcatc
     9541 agatgccgcg caagagttcg ttcacgctgg ttttggcgcg ggtttgcgcg tccacgcgtt
     9601 tgacgatgac ggcacagtaa aggctgtggc tgccgtcttt ggaaggcagg ctgccggaca
     9661 cgacgaccga acctgccggt acgcggcctt ggtagatttc gcctgtggtg cggtcgaaga
     9721 ttttggtgga ttgaccgatg aacacgccca tcgaaatcac gctgccttct tcgacaatcg
     9781 cgccttcgac gatttcggaa cgcgcgccga tgaagcagtt gtcttcaatg atggtgggtg
     9841 cggcctgcag gggctcgagt acgccgccga tgccgacacc gccgctcaag tggacgtttt
     9901 tgccgatttg cgcgcaagag ccgacggtcg cccaagtatc gaccatcgcg ccttcgtcga
     9961 cgtatgcgcc gatgttgacg taagacggca tcagcacggc gtttttggca acaaagctgc
    10021 cgcgtcgggc aaccgcaccc ggaactgcgc ggaagcctgc gtttttgaac tcgtcttcag
    10081 accagtcggc aaatttggtc ggcactttgt cgaagtattt gttcacgccg tcgttgagga
    10141 cttcgttgtc ttggatgcgg aaggacagca acacggcttt tttcgcccat tcgttgactt
    10201 tccactcgcc cacgcccaag cgttcggcga cgcgcagttt gcctgaatcg agttggcgga
    10261 tggtttccaa cacggcttct ttgacttcgg gggcaacggt ggtcggagtg atgtccgcgc
    10321 ggttttcaaa ggcggtttcg ataatgtttt gcaaagacat aatgtttcct tggttcgaga
    10381 tatttcggga cgcttgccgg tattgttcag acggcatggc ggttggaaca aacgtgctat
    10441 tgtgccatac aaacgcccgc ccgtcagccg cgccgcaggc ttgccggatg ccggcgtttc
    10501 ggaatcgggc gggcggcagg catatgtctt tttccgcgcc cgaatcaata cagaaaggcg
    10561 gcggtacttt tatgccgcgc cgccgccttt cagacggcat tcgcggtaaa acgccatcag
    10621 cccttcggtc gaggcatcgt gtacggacgt gccgccttcc agttcgccga tgatggtttt
    10681 tgccaactgt ttgccgtact caacccccca ctgatcgaag gggttgacgt tccatatcgc
    10741 gccttggacg aaggttttgt gttcgtaagc cgccatcagc atacccagat tgcagggcgt
    10801 gaggcggtcg agcaggatgc tgttgctggg gcggttgccg gggaactctt tgtgcggcgc
    10861 gaggcgttcg cgttccgctt cgggcaaatc tgccagttcg gcgcgtgctt cgtctaaggt
    10921 tttacccttc atcagggctt ccgcttgggc aaaggcgttg gcaacggtaa aacggctgcg
    10981 tccgtcctcc gcgccctgcg ccgtcatcgg gacgataaag tcgcagggaa tcaggcgcgt
    11041 gccttggtgg agcagttgga aataggcgtg ctggcagttg accccttcac cgccgaacac
    11101 gatgccgccc gttttgcacg cggcgggact gccgtctgaa gcgcggcttt tgcccaaact
    11161 ctccatatcg agctggttca gccacgccgg cagcaggcgc aggttgtggc tgtacggaac
    11221 ggcggtctgc ccgtccgcgt gctggaaatt gttataccac acggcaatca gtgccatcag
    11281 aacgggaatg ttgcgacgcg ggggcgtgtg gaaaaaatgg ctgtccatcg cgtgcgcccc
    11341 cgctagcagt tcgcggaaac gcgccccgcc aaccgcaacc atcacgggca aaccgacggg
    11401 cgaccagacg gaatagcgtc cgcccaccca gtcgtacatc gcaaacacgc gttccgccgc
    11461 aatgccgaaa ctttgcgccg cttccgtatc ggcagacacc gcgcaaaaat ggcgcgccgt
    11521 ttcggattcc gagaaccccg caccgcgata ccacgccttg accgcctctg cattgagcag
    11581 ggtttccggt gttttaaagg acttgctggc aacgcaaaac attgttgttt cggggttcag
    11641 acggcataag acctcatcca ggcaggcagg gtcggcatta gaaacaaaat ggacggaaat
    11701 ctgccgtctg aacggctcga gcgcctgcac gcacattgcc ggcccgaggt cggatccgcc
    11761 tatgccgatg tggacaaaat ccgcaatccg ttttccggtt atcccctgat ataaaccgtc
    11821 gtccaaactg tgtgcaaact tcaacgcacg atttaactcg cggcggattt cgggcaacac
    11881 gtccctgccg tccgcataaa cggcatccgc accgtcgggc aggcgcaaag ccgtatgcag
    11941 cgcggcacgc ccctcgctgc cgttgacttt cgcacccgtc cgcaaagcac gcattttccc
    12001 ttccaaatcc gccgtctccg caagacggca gagcagttgc agcgtatctt cgccaaaacg
    12061 gtttttgctg taatcgaaca acatcccgtc caaacgctca tgcatacgct caaaacggtc
    12121 gggttcggca gcaaagcggt cgcgcaaaag gatgtggcac gtatcctgat aatggcgttc
    12181 gagcgcatac catgcacggg taaaggcatc catctgtttt ccttgatttt tcagaaccgg
    12241 attaaaatat agcagaatgt agtctaacaa acggcagaca ctttagcgaa tctcaggaac
    12301 accgcccgcc gcgtccaata acaccttatt tccatatggc gaaaatatag tgaattaaca
    12361 aaaatcagga caaggcggcg ggccgcaggc agtacggata gtacggaacc ggttcgcttg
    12421 gtgcttcatc accttaggga atcgtcctct ttgagccggg gcgggccaac gccgtaccgg
    12481 tttttgttaa ttcactatac cctccggccg gcatttgacc cgcaacaata tcctgcaaga
    12541 tttattgtgt gcgcataaat gccggacagc cgcctaaatt aaggtataat cgcatccgat
    12601 tctgtcccgt ttgccgcccg caggcgggat gtcggcggtt ttcatcttac cgggaacacg
    12661 ccgatactgt tttttaacga tgtttcttac attttattcc aattacttta cggggctaga
    12721 atatggctaa aaatggggga ttttctttgt tcgcaaagaa agaaaaacgc tttatctttg
    12781 aaggcaggca ttctgcctcc gacaaactgg tcaacggcga agtatccgcg tttaccgaag
    12841 aagaggcacg caaaaaactg gcaaaacgcg gcatccgccc gttgcagatt acccgtgtga
    12901 aaacaagctc caagcgcaaa atcacacaag aagacattac cgttttcacc cgccagcttt
    12961 ccacgatgat taaagcgggc ctgccgctga tgcaggcatt tgaaatcgtg gcgcgcggac
    13021 acggcaaccc gtctatgacg gaaatgctga tggaaatccg aggccaagtg gaacagggca
    13081 gctcgttgag ccgcgcattc tcaaaccacc cgaaatattt cgaccgcttc tactgcaacc
    13141 tggttgcggc gggcgaaacg ggcggtgtat tggaaagcct gctggacaaa ttggcaattt
    13201 acaaggaaaa aacccaggcc atccgcaaaa aggtaaaaac cgcgctaacc tatccggtat
    13261 ccgtcatcgc cgtcgccatc ggtttggtat tcgtgatgat gattttcgta ctgcccgcct
    13321 ttaaagaagt ttacgccaat atgggcgcgg agcttccccc actgacccaa acagtgatgg
    13381 atatgtccga ctttttcgtc tcatacggct ggatggtgct gatcgcactg ggctttgcca
    13441 tatacggctt ccttaaattg aaggcgcgtt cgattaaaat ccaacggcgt atggatgcca
    13501 tactgctgcg tatgccgatt ttcggagaca ttgtccgcaa agggacgatt gcccgctggg
    13561 gcaggacgac ggcaacgctg tttgccgccg gcgtgccttt ggtcgatgta ttggactcca
    13621 ctgccggcgc ggcgggcaat ttaatctatg aagaagccac ccgggaaatc cgtacgcgcg
    13681 tcatccaagg tctgtctatg acttcgggga tgcgtgcgac ggaactgttc cccaatatga
    13741 tgttgcagat gtcctccatc ggcgaggaat cgggttcttt ggacgatatg ctcaacaaag
    13801 ccgccgaatt ttacgaagac gaggtggaca atgcggtcgg caggctgtcc gctatgatgg
    13861 agccgattat tattgtgatt ttgggcttgg tcatcggtac gcttctggtc gctatgtatc
    13921 tgccgctgtt caacttgggc aacgtggtcg cctgatttgc cgcacagatc cggcgcggat
    13981 tggttctgcg ccggtttgtt tttgctttga atatatcaag gacaaaatat gtctgatttg
    14041 tctgtattgt cgccgtttgc cgtgcctttg gcagcagttt tggggctgct ggtcggcagc
    14101 ttcctgaatg tcgtcattta ccgcgtaccc gttatgatgg aacgcggctg gacggtattt
    14161 gccaaagaac atttaaacct gccgctgacc gacgatgaaa gccgtacctt caacctgatg
    14221 aagccggatt cctgctgtcc caaatgccgt gtgccgatac gcgcgtggca gaacatcccg
    14281 attgtcagtt acctgctcct gcgcggcaaa tgcgcttcct gccaaaccaa aatcagcata
    14341 cgttatccct taatcgagct gctgaccggc gtattgttcg ggctggtcgc ctggcaatac
    14401 ggctggtctt ggattacgct gggcggtttg atactgaccg cgtttctgat ttccctgacc
    14461 tttatcgatg cggacaccca atacctgccc gactcgatga cattaccctt gatctggctg
    14521 gggctgatat ttaatttgga cggcggcttc gtgcctttgc agtctgccgt tttaggtgcg
    14581 gttgccggct atagttcatt atggctctta tgtgcagtgt ataaactgct cacaggaaaa
    14641 accggtatgg gcaacggaga tttcaaactg attgccgcat tgggcgcgtg gctcggcata
    14701 tccgcattgc ccgtgctgat ttttgtttcc tctctgatcg gtttggtcgc ggcaatcgtt
    14761 atgcgcgtcg ccaaggggcg gcattttgcc ttcggccccg cactgacagt ttcgggctgg
    14821 ataattttta cggcaaacga ttccgtatgg cgggcggtca actggtggct gacccatccg
    14881 gtgagatgac agcatgggtc ggactgaccg gcggaatcgg cagcggcaaa tcggcagccg
    14941 cgcaatattt tgccgatttg ggcgtgccgc gcatcgatgc ggacgcggcg gcgcactcgc
    15001 tgacggcttc agacggcatc gccctgccgg aaatcaggcg gctgttcggc gacaccgttt
    15061 tcgacacaca gggtttgttg cggcgcgaca tattgcgtaa agaaatcttt gcctccccat
    15121 cgcgcaaagc cttgctcgaa tccgtgatgt tgccgctgat tttctcagaa atcaaaaaac
    15181 agcaagaaac ctttaccgat gcggtttacg gcattgtcga aattccgctg ctgacggaga
    15241 agcgtcaatt tatcagcctg atacggcgtg tcctgaccat aagcgcacct ttggaaaaac
    15301 gtatcggcag ggtaatggca cgcagcgggc tgacgcgcgg cgaggtggcg gacatcatca
    15361 gccatcaggc atccgaatcc gaacgcctgc tgcttgcaga cgatgtactg ctcaatgacg
    15421 gcagcctcaa aagcctacgt gagaaaacaa tgctcctgca cgcgttttat tcagggattt
    15481 tcgcctcaaa accaacacaa ggaaaacaca atggctgaat cgcggcaaac acgccttcaa
    15541 gtcaaatgtc cgacctgtca aacggcagta gtatggaaac cggaaaacgc attccgcccg
    15601 ttctgttcgc aacgctgcaa actgatcgac ttgggcggat gggcagacgg gaaatatacg
    15661 gtttccggcc aaacggaaag tttgccggaa atatccgaac ccgacggggc ataccgctga
    15721 ccgccccgcc ttcccggcaa acaccctgaa aggtcaaatg ccgtctgaaa caaacacgct
    15781 tcagacggca ttttcattct caaacctaat cgttggtatt tgccgttacc tcttccaatg
    15841 aagtaatgcc ctgcataatt ttcaaaatac cggcccggcg caaatccacc ataccctcct
    15901 tataggcaac gtccaaaata cccacttccg taccgttgtt cataatcaca cgctgcattt
    15961 cttcgctgat gggcataacc tcatacacgc ccgcacgccc cttataaccc tgcccccggc
    16021 aacggtcgca accgacggcg cggtaaagtt tccaatcttt tgcaagatcc tcatcggtga
    16081 aaccgacttc cttcaaagca gaggcagacg ggcgttccac ttcctgtttg cagctcgaac
    16141 acagcctgcg taaaagacgc tgcgccataa tcaggctgac cgaactggca atattaaacg
    16201 gcgcgacacc catattcagc atacgcgaca acgtcgccgg cgcattattc gtgtgcagtg
    16261 tggaaaacac catatgccct gtttgtgccg ccttaatcgc aatatcggca gtttccaaat
    16321 cacgaatctc accgaccata atgatgtccg ggtcctgacg caggaaagac ttcaaagcag
    16381 cggcaaaagt cagaccctgc ttatcattga cgttaacctg attgatgccc ggcaggttaa
    16441 tctcggcagg gtcttccgcc gttgcaatat ttaccgactc cgtattcaaa atattcaaac
    16501 aggtatagag cgacaccgtc ttacccgaac ccgtcgggcc ggttaccagc accatcccgt
    16561 aaggacggtg aatcgcttcc aacaacaatt ttttctggaa cggctcaaaa ccgagctggt
    16621 cgatgttcaa agacgcggca tcggaattca aaatccgcat cacgaccttt tcgccaaaca
    16681 gcgtcggcaa tgtgctgaca cggaaatcga caggcttgcc gcccttttga aaggtcagct
    16741 gcatcctacc gtcctgcggt atccgttttt cggaaatgtc caaacgcgac attaccttaa
    16801 tccgggaagc aagctgcccc cttaccgcaa tgggcggctg aaccacctcg cggagctgcc
    16861 cgtccacacg gaaacggata cgcgcattgt gttcgtaaaa ctcgaaatgg atgtcggatg
    16921 ccccgctacg caaggcatcc gacaaagttt tatggataaa cctcggaaca gggccgtctt
    16981 ctgcctcctc gttgtcgata tacagggtgt ggctttcctc ttcctcttgc ccctccccaa
    17041 gctcctgaag cagcgatgtc gaacgcgaac ccacccaatc gagcaaaccc gccaactggt
    17101 catcctcgac aatgaccaac tcaaccgcaa tccctgcggc agaaacggtt ttctgaattt
    17161 gcggcatctg ggtcggatcg gaaaccgcaa aaaatacttt gtcgccccga cggaaaaccg
    17221 gcacacagtg gaactccacc atctgctcct ccgtcaacac ccccatcagc accctgtggc
    17281 gcggataatg acgcaaatca agaatcgaat aactgaacac cctcgcaatc aatgccgcaa
    17341 gcgacttggg cgaaatgaca ccgtctgaaa acagcatcgg caacacttcc ttacccgcct
    17401 gcgactcatt gtagtaatgc tcggcctgct caacagtaac cacctggttt tgaaccagaa
    17461 tcctcagcaa acctacgctc atacgacctt atccccaaat ttattcattg ttatacctgt
    17521 acagatttta tcataaaaca acagatttcc aagcctgaac atcttttacg aagcctgaaa
    17581 atcatttcac gattctgccg tcctaaaggt cggtttttca agcaggaaga aaaagttttc
    17641 agatggcaaa aaagccctcc agcactgaaa ggccttatat cggaaacttc ccgcaacacg
    17701 ggaaacagac aaatgcaaac cgtcaaacct cgccaacagg aatcgaacct gtattttacg
    17761 cttaggaggc atacgttcta tccgttgaac tatggcgagc cgaaatgaaa agggagattt
    17821 taaacctttc cggcaacaaa agacaagatg tcggccgcaa tcagcggccc gacagggata
    17881 ccgcccataa atgccacccc gatgaccgta ccgatcagca agcccgttac caataccggc
    17941 tgccgtccca tcaaaggcac gccgcatccc gccagccaag ccacgaaaat accgataaaa
    18001 acggcggata tcattttaaa attcaaaaat tcggcaacag gaggaacctg cgcctttcct
    18061 gaaaccaacg gactcaaaac ccctatggtc aaaagaatga taccgaggtt caacccgtgc
    18121 ttctcgacca acgggacaaa ctgtaccaat gccgtctgct gcatcagcag caatatggtt
    18181 gccgagacgg taatcgaatt gttgttgctg accaccccca acagaatcag cgtaaccaga
    18241 aacaggggaa caaaactgaa attcatcggt tacgacaccc tgaccaaaga ttccttcgcc
    18301 agctgaatca tagcatcctt cacttcgctg tcggacaggg catccaacgc ggcaacggca
    18361 caatcgactg ctttgcgcgc ctcgcctatc gaatatgcca aagcatccga acggacgaca
    18421 taatcgcgga ttttttcaaa atagccgcga tctgcatttt ccaaagcagt acgcacatcg
    18481 ttcgcaacct gttcggaacc ctgacgcatc agataaatca aaggcagggt cggttttcct
    18541 tccgccaaat cgtcgccgac gtttttacct gtttcttcgg tttcccccga ataatccagc
    18601 acatcgtcaa taatctggaa tgccgtaccg acgtacatac cgtagtcttt caaggcctgt
    18661 tcgtgcccgg gggaagcttt gcccaaaatt gcgccgactt gagcggcagc ttcaaacaat
    18721 tttgccgttt tatattggat gactcggata tattgttctt cggtaatgtc cgtattgccg
    18781 atgttcatca gctgcatgac ctctccctcg gcaatgatgt tggtcgcatc cgccatcact
    18841 tccaaaacgc gcatactgcc cgaggcaacc atcagttgaa atgcacgggt atataaaaag
    18901 tcgccaacca acactgccgc cgcattgccg aacagattgt ttgccgttgc ccgcccacgg
    18961 cgcaaatcgc tttcatcgac gacatcgtcg tgcaggagtg tggaagtgtg gataaactcg
    19021 accatcgctg ccagcgaata cagtttctcg tcatcataac cgaccgcctt acccgccaaa
    19081 atcgtcataa tcggacgcag gcgtttgccg cccgcgctga tgatatatgt accgatttgc
    19141 gaaatcagtg cgacatcgga ttgcaccgca cggttgatga cttcattgac tttggcaagg
    19201 tcttcaggca gatgtcgctg gaaatagggc agattctcga gcatggcaga ctctcaggtt
    19261 tacgtttccg tcaaacggca gataccgctt gaaagctgat tcaaatagcg ggcaattata
    19321 tcagcaaagc catcagggta catcttttca gacggcatat gattgcccga accgcgcaaa
    19381 ctttgacaaa acaggcaaat aacaatacaa tgcccaattt cgcacaatgg tgtgcggata
    19441 ttaaccataa cccttatgga gttgagtatg tacgcggtcg taaaaaccgg cggcaaacag
    19501 tataaagttt ccgtcggcga aaaattgaaa gtagaacaga taccagccca actcgacagc
    19561 caaatcgaac tgaccgaagt tttgatgatt gctgacggcg aatctgtaaa agttggcgca
    19621 ccctttatcg aaggtgcaaa agtaacggct aaagtagtgg cacacggtcg tggcgaaaaa
    19681 gtccgcatct tcaaaatgcg ccgccgcaaa cactaccaaa aacgccaagg ccaccgccaa
    19741 aatttcaccc aaatcgaaat cgtggcaatc gcctaacctt aaaacttagg agcattacaa
    19801 atggcaagta aaaaagcagg cggcagcacc cgcaacggtc gcgattcaga agccaaacgc
    19861 ttgggcgtta aagcctacgg caacgagctg attcccgcag gttccatcat cgtacgccaa
    19921 cgcggtacca aattccacgc aggcgacaac gtaggtatgg gcaaagacca cactttgttc
    19981 gccaaaattg acggttatgt cgaattcaaa accaaaggcg cgctgaaccg taaaactgtc
    20041 agcatccgtc cttacaccgg ttctgaagaa taattcaacc gatacctgaa gccgcatctt
    20101 ttcacgatgc ggctttttac ataccccata cccgatttga tacacattaa gctgaaagta
    20161 aaaatccgca tacaccctcc cctgcatatt tcttcaacaa cgggttttga tataatcgcc
    20221 tatctgttac agatagttca aacggcattc aaacccttac aaatgccgtc tgaaatcatc
    20281 ggcatccgcc tatgcaaagg atattttatg agtttacaca gcgacatcct cgtcgtcggc
    20341 gcaggcccgg cgggtttaag ttttgcagcc gaacttgccg gcagcggttt gaaagttact
    20401 ctgatcgaac gcagcccact gactgttctg caaaatcctc cctatgacgg acgcgaaatc
    20461 gccctgaccc atttttcccg cgaaatcatg cagcgtttgg gtatgtggga caaaattccc
    20521 gaaaacgaaa tctacccctt gcgcgatgcc aaagtgctga acggacgttc cgactaccag
    20581 ctccactttc cccaaccgac tgaagcgcgc ggcgagcctg ccgactgttt gggctatctg
    20641 atttccaacc acaatatccg ccgcgccgcc tatgaaatcg tatcacaact cgacaatgtc
    20701 agcattctga ccgatacggc cgttaaagaa gtcaaaacat ctgataatga agcgcaagtc
    20761 tttttggaaa atggagacat tctaaccgcg cgtctccttt tggcagcaga cagccgtttc
    20821 tcacaaaccc gccgacaact cggtatttct tcagatatgc acgactacag ccgaaccatg
    20881 ttcgtctgcc gcatgaaaca taccctttcc aaccaacata ccgcttacga atgcttccat
    20941 tacggacgca ccatcgcact gctgccactg gaaaagcgcc tgaccaatac cgtcattaca
    21001 gtcgataccg ataaaatcaa cagcgttcaa aacctttcgc ccgaagaatt ggcggcaagc
    21061 gtaaaagaac agctcaaggg caggctcggc gacatggaac tggtcagcag catccaccat
    21121 tacccccttg tcggcatgat tgccaaacgt ttctacggca aacgcagcgc gctgatcggc
    21181 gatgcggctg tcggtatgca ccccgtaacg gcacacggct tcaacctcgg gctttccagc
    21241 gcggatattc tggcaaaact gatactcgaa gccgaacagc gcggtcaaga catcggcgcg
    21301 gcttccttgt tggaaaaata cagcagcaaa catatgctgc acgcccatcc gctttatcac
    21361 ggcaccaata tgatgctgaa actgtttacc aacgaaaccg cgccggcaaa actgttgcgc
    21421 ggtttggtat tacgagccgg caacaacttc ccgccgctga aaaagctgat taccaaacaa
    21481 ctgaccggtt gaaaactgcc gaaatgccgt ctgaaacgtt tcagacggca tttttgtatt
    21541 atgaaccgtt ttgcggtttc catatccttg gcagacggtt tgcagcagat tcgcatacgg
    21601 cagatgtttc aagcagaaag gaaacacaat aaaaataaaa aagcctccga acagtcggag
    21661 gctttgcttt caaactgaaa ttatttcagt ttggtttctt tgtacactac gtgtttgcgg
    21721 gcaaccggat cgaatttttt gatttccagt ttgccgggca tagtgcgttt atttttggta
    21781 gtggtgtaga agtggccagt acctgcaccg gattccagtt tgattttatc gcgcattgca
    21841 gtaatcctta attaaatagt gtttaaatta agcttcgccg cgagcacgca aatcagccaa
    21901 tacgacatca atgcctactt tgtcgatggt acgcaatgca gcgttggaaa cgcgcaggcg
    21961 aacccagcgg ttttcacttt ctacccaaaa acgacgtgat tgcaagttgg gcaaaaaacg
    22021 gcgtttggtt ttgttgttgg cgtgcgatac gttgttgccg gacatcgggc gtttaccggt
    22081 cactttgcaa actcgtgcca taatcagtct ccaaacttct aaaatagtaa aacgcgattt
    22141 ataccacgaa aaacaatgcc gtttcaagtg ttttaaagaa aaacagatgt tttgcgcggg
    22201 taagccgccc gagtgccgca tctgcggaac gtttgcaggt gcgaaacaga tggggcgcga
    22261 ttatagcgga tttcgggagc ttctgcattc aaaatgccgt ccgaacatat ttccagtatt
    22321 ttcagacggc atttcaagtt ttcaaatccc tcaatgtcca ccgccgccgc tgtggaacgg
    22381 cggtttcgcc agccagacaa tgggaatcag gacgataaac aaaatgctgc cggcgaggaa
    22441 tatttcgttc gatccgatga tgaagccctg ttgcgtaatg gtattgttga tgatgcctag
    22501 agtttgaccg tcggaaatgc cttgttggga caaatgcgcc gccgtttcgt gcaatgttgc
    22561 ggaatagggc gtgatgtgtt cggcaaaacg cgtgtggtgc aacgcttcgc gccgttccca
    22621 cagggtgctg acgacggata cgccgacacc gcccatcagc acgcgcagga aattcgacag
    22681 gctgccggcg gcggcaatct gtccgccctt catgtgcgac agtgtgatgg tggtcagcgg
    22741 caggaagaac atggcaacgc ctaccccctg ccaaaactgc ggccagatga cgttgccgat
    22801 atccatgtcg gcataaaaat cagtgcgcca ataaaaagtg aaggcaaagg tcaggaagct
    22861 ggcggttacg agcaggcgca tatcgatttt attgccgaac ctgccgatta acggagatag
    22921 gaagacgggg aggatgccga cgggcgcggc agcaagccct gcccacgcgg aggtatagcc
    22981 caagttggtc tgcaacacta acggcagcag ggtcagcgtc cccatataca ccatgaaacc
    23041 caacgaggtg gcaattacgc cgacggtaaa attccgatct ttaaacagcg ataaatcgac
    23101 aatcggatat ttttctccca attcccaaac aataaaatac gacaagcaca ccagtgcgac
    23161 tacgcccaag gtaatgattt ctccagaggc gaaccagtca agttccttac ccctgtccag
    23221 catcatctgt aacgcgccga taccgaccat catcagcgtc agcccgacat agtcggtcgg
    23281 cgttctgacg gttgccgttt cgcggtgttt caaatgtttc catgtaatcc atgccgatat
    23341 gatcccgatg gggatattaa tgaagaaaat ccaaccccaa tgccagtttc cggaaatcca
    23401 accgccgagt atcggcccga ggacaggggc gacaacgacg gtcattgccc acaatgccag
    23461 tgccagcatc cgttttgcgg gcggatagga tgccattaac aggctttgcg acaggggaat
    23521 caacggcccg gcgataaagc cctgcaaaat gcggaaaaca accagcgact gaaggttggg
    23581 cgcgatgccg cacagccacg atgcgatgac gaaaccggcg gcggcggcgg taaacaattt
    23641 gacctcgccg atacgttttg ccaaaaagcc cgtcagcggc acggaaacgg cgtttgccac
    23701 ggaaaaagaa gtgatgaccc acgtcccctg cgtggttgcc gcaccgaggt tgccggcgat
    23761 gacggggacg gcgacgttgg cgatggtcgt atctaaaacc tccataaata cggcaagccc
    23821 caaagacagc gtaacccacg ccaatgccgc acccttaagc ggtggataat ccattgtgtc
    23881 ctccgtttca gacggcatca gggcgcggat tgcccgagga tttcatcgac cgtccgatcg
    23941 acttccgacc agtcggtact ttccatttcc ggcaatgccg cacccggcgt ttttgaaaca
    24001 ggcgcgcctg cggcggaagt atccacttta accgtcatcg acaaaccgat acgcaacgga
    24061 tgcctgtcca catcttcgcg gttcagcacg atacggacgg ggacgcgctg caccacttta
    24121 atccagttgc ccgttgcgtt ttgcgccgga atcagcgaaa acgcgctgcc cgtacctgcc
    24181 gaaaaccctg ccaccctgcc gcgataaaca atttgtttgc cgtacaaatc ggacaccagc
    24241 tcggcaggct gtccgatttt catatgccgc aactgcgtct ctttaaaatt ggcatccacc
    24301 cacacatccg acagcggcac caccgccatc agcggcgcgc ctgccgccac ctgctgcccg
    24361 acctgcaccg aacgcttcgc cacctgaccg tccgccggcg cgcggacttg cgtccgccga
    24421 aggttcagcc acgcatcttt caacctgccg attgccgtct gaacctccgg ctgttcgcgc
    24481 aaagaaacat ctccgcccaa agccgcgcgt gccgaagatt cttccgccaa agccgcctta
    24541 accgccgcct gcgcctgaga cactgccgta cgggcgtgtg ccagctcttc ggcggacacc
    24601 gcacccgatt ccgccaaagc ggaccggcgg cgcaaatcat cctgcgcgcg cgccaaatcc
    24661 gcacggcgca aggcaacctg cgcccccgcc tgcgaagtgg cggcattttg ccggcggttt
    24721 tggcgcaccg cctgaaccaa ctcgtttttt gcccgctcgt aagccagcac atcattatcg
    24781 tcatccaaca ccgccagcac gccgcctttt ttcacggcat ccgtatcgtc gtgcaaaacc
    24841 ttccgcaccg tgccgccctt ttgcggcgta acctgaacca cgcgtccggc aacataagcg
    24901 tcttccgttt cctcttcgtg ctgccaccat aaaaaaaacg ccgatccggc ggctgcggcg
    24961 gaaagcgcga acagcagcgt caatgccgtc aggcggcgtt tgcgtttgac ttgcgtgttt
    25021 tgaagttttg tttcgtctgt gtgcgtttcc ataagattgg gttccgaatt tcctgatttc
    25081 atataaacaa tgttctttca ataggaaata gattttatat ccgcgcaata acgcaatcaa
    25141 gtcagtattt tagatgcaat attcaaaaaa caacgcttca ggcggcaaaa ctgaaaatat
    25201 atttcgttta tatatgacat ggcaaacacc gccgcccgca ccatgccgtc tgaagaaaac
    25261 tacacagata ccgccgctta tattacaatc gccgccccgt ggttcgaaaa cctcccacac
    25321 taaaaaacta aggaaaccct atgtcccgca acaacgaaga gctgcaaggc atctctcttt
    25381 tgggtaatca aaaaacccaa tatccgaccg gctacgcgcc tgaaattctc gaagcattcg
    25441 acaacaaaca tcccgacaac gactatttcg tcaaattcgt ctgccccgaa ttcaccagcc
    25501 tctgcccgat gaccgggcag cccgacttcg ccaccatcgt catccgctat atcccgcaca
    25561 tcaaaatggt ggaaagcaaa tccctgaaac tctacctctt cagcttccgc aaccacggcg
    25621 attttcatga agactgcgtc aacatcatca tgaaagacct catcgccctg atggatccga
    25681 aatacatcga agtgttcggc gagttcacac cgcgcggcgg catcgccgtt catccgtttg
    25741 ccaactacgg caaagcaggc acagagtttg aagcattggc acgcaaacgc ctgttcgagc
    25801 acgacgcaca ataaaccatc cttcccatat ttcagacggc attccttcga tacggtatgc
    25861 cgtctgaacg cctttccgaa aggtttttat gtacgcattg accgccgcac agcaacagaa
    25921 ggcactcttc cggctggtgc ttttccatat cctcatcatc gccgccagca actatctggt
    25981 gcagttcccc ttccggattt tcggcatcca caccacttgg ggcgcgtttt cctttccctt
    26041 catcttcctc gccaccgacc tgaccgtccg cattttcggt tcgcacttgg cgcggcggat
    26101 tatcttttgg gtgatgttcc ccgccctttt gctttcctac gtcttttccg ttttgttcca
    26161 caacggcagt tggacgggct tgggcgcgct gtcccaattc aacacctttg tcggacgcat
    26221 cgcgctggca agttttgccg cctacgcgct cggacaaatc cttgatattt tcgtattcga
    26281 caaattacgc cgtctgaaag cgtggtggat tgccccggcc gcatcaaccg tcatcggcaa
    26341 tgcactggac acgttagtat tttttgccgt tgccttttac gcaagcagcg atgaatttat
    26401 ggcggcaaac tggcagggca tcgcttttgt cgattacctg ttcaaactta ccgtctgcat
    26461 cctcttcttc ctgcccgcct acggcgtgat actgaatctg ctgacgaaaa aactgacggc
    26521 cctgcaaacc aaacaggcgc aagaccgccc cgtgccctcg ctgcaaaatc cgtaaaacag
    26581 ctctgtaggg aatcgggacg gttgggtttt cttttatttg caaacactgc cggaacggat
    26641 aaatccggat tcccacccac ttcgtcattc ccgcgaaagc gggaatccgg tgcgttgggg
    26701 ttcagtcatt tccgataaac tcctgccgca ttgtatttct agattcccgc tttcgcggga
    26761 atgacggcgg atgggtttcc gtgccgtggt gtccggattc ccgccggtac gggaatgacg
    26821 gcagcgcttc cttggcttag ttttatttta agccactata aatgccgtct gaaaacctgt
    26881 ttgcagattt tcagacggca ttgttttcgt cgccggttca aacttcaatc cgattgcgac
    26941 acggtaaccg gtatgccgcg caagcgcgca agcagcgcgt ccgccttctg tttctgcgcg
    27001 gcaaacgccg ccttgtccgc ctgcgtcaat tcgggtgtcg gcaatgcgac cgaaacagga
    27061 ttgacgggct gcccgttgat gcgcgcctcg taatgcaggt gcggcccggt cgaacgccct
    27121 gtcgaaccga caaaaccgat gacctcgccg ccgcgcacat tgccttgtgc ctgcgaaaac
    27181 gcgctcaagt gcgcgtacag cgtttccaca ccgttggcgt ggcgtatcat caccgcgttg
    27241 ccgtatccgc ccttccggcc tttaaaggta atcacgccgt cggcggaagc cctgaccggc
    27301 gttccctgcg gtgcggcata atcgatgccc gtgtgcagcc gccatgtgtg caggatgggg
    27361 tgcatacggt agccgaacgg cgaagaaatg cgcgtataga ccagcggctc gatgttgaag
    27421 ccgccttttt cctgcaacac cctgccgtct tcatcgtaat aattgccgcc ccctccgcct
    27481 tccttgtccg aacggtaata gaacgcctga tgggttgtgc cgcccttgac aacttccgcc
    27541 gccaaaatat cgcccgccgc cacctgctgc ccgtggaaat acaggctgtc gtaaagcagg
    27601 cgcacggcat cgccttcctt caaaccgtca aggctgaagc ggccggcaaa aatcccgctt
    27661 aaggattcgc ggatttcgac gggcacttcc gcccgcgcca gcgaaccgcg cgccgacgtt
    27721 ttgaccacga ccgaacgcag cgtcggcaaa accttcatat ccgcatcaga agccgaccgc
    27781 cgccatatgc cgcctttttt ttccaaagcg accagattgc gctcgccgtc ttcgtcggta
    27841 aaaaactgca cttcgcgcgc actgccgtcg ccgccgacca aaacatgaac cgactggtcg
    27901 gcacgcaaat gccgcaaatc ggcttcgccg ccatattttt ccgtgattcg ggcaatctcg
    27961 tcccgcgcca tacccgaacg cgccagcacg tccgccagcg agtcgcccgg ctgcaccgcc
    28021 tcctgcaccc aatatgccgt ctgaacgccg ctgccgcccc aagacagcgg cggcagtttt
    28081 tgttccacgc gctgcggtct gacacgctcc gtcccctctg tcgaagcaat gtatgccgcc
    28141 gacaccaaaa taatcgaaac ggcaagcgcg cgcagcgcgt atttccgatg ttttgccgaa
    28201 agtgggaaga cagccatatt gttccttttt gacggcgttt gcgccgaatc ctctataata
    28261 tccgcccgat cctcatttgt caggcatcaa acgatgaaac tcaccctcgc cctcccctcg
    28321 ctcaatttgg atgaagacga aatccgaata cctctatgcc tgcacgcctt caacaaaatc
    28381 ctgcaatacg gctcgccgca ccgccaaagc tgcacggctt cggcatttta cgcacgttat
    28441 ttgtggtgcg gaagactggc acaacgcccg gcgcaaagcc tgaatatgcc gtctgaaacc
    28501 gtcgcgctcg ccacgcccgt ctggcaaaaa atggggctgc atcaggcaaa tgtgctgacg
    28561 gcggaatatt tggatgtcgg tacggatgaa gcggaacggc tctgccgcga tctgtcggcg
    28621 ttttacggcg acattccgtg gcgtttcgtt cccgtcctgc ccgaattatg gctggtttcc
    28681 cttccccgcg cgtaccgctg gggcgcgaag cccgttttgg acttgggcgg actgttgggt
    28741 gcggacaatc aaccggacgg ggaagacgcg ttggaatggc tgcgcgtcca aaccgaaatc
    28801 caaatgtggc tgaacgctca ccccgtcaac cacaaccgca aaaaacgcgg gctgcccgaa
    28861 ttgaacggct tatggctttg ggacagtctg cacggaagcg cacaaggcgg cacgcttttc
    28921 gccgatacgg tttggagccg ttttcacccg aaccgccgag cattgcccga cagcttccga
    28981 gcttatgcgg aaacggcggc acacctgccc gatacgcacc acatcctgtt tatggacgat
    29041 ttgcgcctga ccgccctgac aggggacagg gagcggtatg cggcaatatt gcagcagtgg
    29101 gaagaacgct ggttcgcacc gctttacgaa gccgtccgca ccggcaaaat caaacggctg
    29161 gacatcgcca cggacggaca acacggcggt acgctgacct tcaaacccac agacaggcgg
    29221 aagttttggc gctgcacgaa aacctttgac ggaatctggt aaacccgccc gccgcaaccg
    29281 cgacaatgcc gtctgaagcc cgaatcgggt ttcagacggc attttacccc gcatcgccgt
    29341 tatgccggaa aaaccggaat acgtttccgc acacgcggcg gcacgcaaaa caccgcccga
    29401 aagccgccga tgcccgccgt gacaccgccg ccggcagggt ttccgccgaa aaggaacagc
    29461 aatcagaacg ttttgttaaa ctcgacaaac gccctgtttt tctcgtattc gttaaacaca
    29521 tcgttgctcc acgtttcgcg gtgcgacagc gtcaggcgcg gcgtgatgcc tttgaaatgc
    29581 aatgcccggt gccaaaggct caaggatgtg tccgattctt tatccctgcg cctttcccct
    29641 ttaaaactgc tgaagaagcc gggtttttca taatgccgtt tcgccacgcc gaggcggaac
    29701 agcgaagaca ggccgctgcc gccccattcc tgcccccagg caaagcgcag gccgtaacgg
    29761 ttgaaattgt cgccacggtc ggcggggttg cgctcgcggt aaaaatccaa accgcccgtc
    29821 caatattggc gcgcattccg gtaaaacacc agcgaattgg aaatttgcaa atgggtattg
    29881 tcggaacgcg cccggcgcgt attcttcaaa cgcccccact ccgccgaaga cagcgtttgc
    29941 catctcgggg tttgccaacg gttgaaataa aggcgtgcgc cgttggcgta agaataagcg
    30001 tcgttgccgt aggtgcggcg ttcgtggaac actgccagcc cgacatcttt acgccggtcg
    30061 gcaaaaccga tgccgccgga aacacctgcc gtcatatcgt tgaatttctt attccccgga
    30121 taaaccctgc cggacacgtc gccgcccgcc gtcgtgtacc agccgttttt cagcgaccat
    30181 tttttctccg cgccgaaccg gtaattgacc gccgtgccgt ccacctgttt cgggaaagtc
    30241 caattgccgt actgctgctg tttcggggct tggttgatat tgtgttcgcg ggtaatgctg
    30301 aaaccgccgt ttaccttcca cgcatcgcgt tcgcgcaatg ccttgcggta cagctcgacc
    30361 tgctccataa gctgcggcgg cagatcttct gttttcaggc ggtcgaactg gtctgccgcc
    30421 gcctcgttct gcctgtcttc aaacaatgcc gccgccaaac gcatacggac ggcgggcgcg
    30481 tcgggttggg cggcaatcaa ttcccggtaa tg
//
LOCUS       DAXDSD010000014        28684 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXDSD010000014 DAXDSD010000000
VERSION     DAXDSD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28684
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.014"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..191)
                     /locus_tag="ACFB2Q_000478"
     CDS             complement(<1..191)
                     /locus_tag="ACFB2Q_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC0917579.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIGNLGRLDPTLKVKN"
     gene            complement(197..478)
                     /locus_tag="ACFB2Q_000479"
     CDS             complement(197..478)
                     /locus_tag="ACFB2Q_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917580.1"
                     /translation="MGLPYQNKDFRNMRLSDYILNNKININNLSDKQVQEIIDYFSSE
                     IDDWLLASEKELKDYDVYLYKLVLDGQQYVQGIRKSVLYDFLMENQKDS"
     gene            complement(471..689)
                     /locus_tag="ACFB2Q_000480"
                     /pseudo
     CDS             complement(471..689)
                     /locus_tag="ACFB2Q_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(796..1209)
                     /locus_tag="ACFB2Q_000481"
     CDS             complement(796..1209)
                     /locus_tag="ACFB2Q_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC0917581.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(1226..2215)
                     /gene="pheS"
                     /locus_tag="ACFB2Q_000482"
     CDS             complement(1226..2215)
                     /gene="pheS"
                     /locus_tag="ACFB2Q_000482"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC0917582.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(2456..2815)
                     /gene="rplT"
                     /locus_tag="ACFB2Q_000483"
     CDS             complement(2456..2815)
                     /gene="rplT"
                     /locus_tag="ACFB2Q_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC0917583.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(2828..3025)
                     /gene="rpmI"
                     /locus_tag="ACFB2Q_000484"
     CDS             complement(2828..3025)
                     /gene="rpmI"
                     /locus_tag="ACFB2Q_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC0917584.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(3172..3693)
                     /gene="infC"
                     /locus_tag="ACFB2Q_000485"
     CDS             complement(3172..3693)
                     /gene="infC"
                     /locus_tag="ACFB2Q_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC0917585.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(3711..5624)
                     /gene="thrS"
                     /locus_tag="ACFB2Q_000486"
     CDS             complement(3711..5624)
                     /gene="thrS"
                     /locus_tag="ACFB2Q_000486"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC0917586.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     EDMPEVEAMGIYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQTI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(5677..5753)
                     /locus_tag="ACFB2Q_000487"
     tRNA            complement(5677..5753)
                     /locus_tag="ACFB2Q_000487"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5717..5719),aa:Val,seq:gac)
     gene            5932..7047
                     /gene="tgt"
                     /locus_tag="ACFB2Q_000488"
     CDS             5932..7047
                     /gene="tgt"
                     /locus_tag="ACFB2Q_000488"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC0917587.1"
                     /translation="MLKFTLHKKDGLSRRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGVD"
     gene            7057..8139
                     /gene="hemH"
                     /locus_tag="ACFB2Q_000489"
     CDS             7057..8139
                     /gene="hemH"
                     /locus_tag="ACFB2Q_000489"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC0917588.1"
                     /translation="MPSEAASDGILPPYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYAASSSGAAVDKMCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALTGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            8330..8798
                     /locus_tag="ACFB2Q_000490"
                     /pseudo
     CDS             8330..8798
                     /locus_tag="ACFB2Q_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
     gene            complement(8795..10516)
                     /locus_tag="ACFB2Q_000491"
     CDS             complement(8795..10516)
                     /locus_tag="ACFB2Q_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFC0917589.1"
                     /translation="MNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAGEEG
                     VGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATVLPL
                     GLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGANDP
                     MAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNLAEG
                     LYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAWLAQ
                     ATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAYISW
                     LGLRGAVPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVAMPN
                     KPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIRNGS
                     RIEMQSDTVLQAEDLAWYILPDGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAARSG
                     DLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGGGNIGS
                     MGLKVPR"
     gene            10720..11460
                     /locus_tag="ACFB2Q_000492"
                     /pseudo
     CDS             10720..11460
                     /locus_tag="ACFB2Q_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            11529..13067
                     /gene="lnt"
                     /locus_tag="ACFB2Q_000493"
     CDS             11529..13067
                     /gene="lnt"
                     /locus_tag="ACFB2Q_000493"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC0917590.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            13331..14194
                     /gene="rpoH"
                     /locus_tag="ACFB2Q_000494"
     CDS             13331..14194
                     /gene="rpoH"
                     /locus_tag="ACFB2Q_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC0917591.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(14351..14827)
                     /locus_tag="ACFB2Q_000495"
     CDS             complement(14351..14827)
                     /locus_tag="ACFB2Q_000495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFC0917592.1"
                     /translation="MESRAFVAARKGNVSSKISKTRTGYGFFAKMIFASRWLQVPIYA
                     GLTVVRAICAYKFLKSLKHLVMNLDVSDENAIMLAVLNLIDVVMIANLLTMVQIGGYE
                     SFVSRLRIDDHPDRPEWLSHVNAPVLKVRLSMSIIGIHPSICSKHLSIPPICRKSS"
     gene            14927..15811
                     /locus_tag="ACFB2Q_000496"
     CDS             14927..15811
                     /locus_tag="ACFB2Q_000496"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC0917593.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENGIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(15902..16417)
                     /locus_tag="ACFB2Q_000497"
     CDS             complement(15902..16417)
                     /locus_tag="ACFB2Q_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC0917594.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(16493..16912)
                     /locus_tag="ACFB2Q_000498"
     CDS             complement(16493..16912)
                     /locus_tag="ACFB2Q_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917595.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(17033..18031)
                     /gene="holA"
                     /locus_tag="ACFB2Q_000499"
     CDS             complement(17033..18031)
                     /gene="holA"
                     /locus_tag="ACFB2Q_000499"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC0917596.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(18033..18512)
                     /gene="lptE"
                     /locus_tag="ACFB2Q_000500"
     CDS             complement(18033..18512)
                     /gene="lptE"
                     /locus_tag="ACFB2Q_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC0917597.1"
                     /translation="MNKIFLTAAALMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAE"
     gene            complement(18564..19343)
                     /gene="pgeF"
                     /locus_tag="ACFB2Q_000501"
     CDS             complement(18564..19343)
                     /gene="pgeF"
                     /locus_tag="ACFB2Q_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC0917598.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            19496..19720
                     /locus_tag="ACFB2Q_000502"
     CDS             19496..19720
                     /locus_tag="ACFB2Q_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917599.1"
                     /translation="MMPLYTFSELYMLQQGTAHQAPHCVLPERGCPPIRFYRYKQTGF
                     NRKGMGIKSISDTSRAMPSENQSPLSDGIV"
     gene            19797..20744
                     /locus_tag="ACFB2Q_000503"
                     /pseudo
     CDS             19797..20744
                     /locus_tag="ACFB2Q_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(20897..22021)
                     /gene="rluD"
                     /locus_tag="ACFB2Q_000504"
     CDS             complement(20897..22021)
                     /gene="rluD"
                     /locus_tag="ACFB2Q_000504"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC0917600.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPPELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MDLDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            22020..22823
                     /locus_tag="ACFB2Q_000505"
     CDS             22020..22823
                     /locus_tag="ACFB2Q_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC0917601.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(22893..25112)
                     /locus_tag="ACFB2Q_000506"
     CDS             complement(22893..25112)
                     /locus_tag="ACFB2Q_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC0917602.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVTDMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     GLWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VSIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            25144..25419
                     /locus_tag="ACFB2Q_000507"
     CDS             25144..25419
                     /locus_tag="ACFB2Q_000507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917603.1"
                     /translation="MIKQSHKTVITRFENDKNANEKPQYYPRQRQTFHRHSRKSGNPK
                     TKSRRNLSERTEPQKPASHLRLNNRTKKAEIKTSGFFFKLAIKHQNE"
     gene            25498..>28684
                     /gene="iga"
                     /locus_tag="ACFB2Q_000508"
     CDS             25498..>28684
                     /gene="iga"
                     /locus_tag="ACFB2Q_000508"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC0917604.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKRGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWSASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQHDNAGTVKG
                     NGEHHWKTTGTNSHIGSTAVRLANNEGDANNGQNVTFEDNGTLVLDQNINQGAGGLFF
                     KGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTDHASTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVAENNDWVFMGYTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGANLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALN
                     SFDATRINGNVNLNQNAALVLGKAALWGKIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKESASGHY
                     QLHVQNKTGEPNQEGLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRLY
                     NPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQDIVVAPPSPQANQAEEAKR
                     QQAKAEQVKRQQAEAGRKSAELSAKQRAGEEE"
BASE COUNT         7360 a         8123 c         6755 g         6446 t
ORIGIN      
        1 atatttttga ctttcagcgt aggatctaaa cgacccaaat ttcctatctt cataccgagt
       61 gcggacggtg tcctgcccag tatttcagca tattcttgaa taacaggatt gtccttacta
      121 accttttgaa aaggaatgat gtaataaaga taaagggcaa ccagggtttc atctctagtc
      181 cattttttca tttttatcag ctatctttct gattttccat aaggaaatcg tacaatacag
      241 attttcttat tccctgaaca tattgctgtc catctagtac taatttgtat aggtatacat
      301 cataatcttt taactccttc tcgcttgcca ataaccagtc gtctatttct gaagaaaaat
      361 agtcaataat ttcttgtacc tgtttatcag ataaattatt tatatttatt ttattattca
      421 aaatataatc agaaagcctc atatttctaa aatctttatt ttgataaggt aaacccacac
      481 agcacatcaa attgcttcgg aatgtattgt ttgatttctt ccaaagtaat atcgccaaac
      541 ggcacttctc cgaaattggc ttcgtacgtc agtttggctt tttcgtccca ctcgctcgaa
      601 aacacgtatt ccccgcccaa gttctgcatc gcgatgcgga agcccccgat acctgcgaac
      661 aagtcgataa aggtaaattt gggtttcaag gccgtctgaa aagtatcgtt gttttcaaaa
      721 aggcggtatt gtgctgcttt ttcttttaac cgttcatcct gcccatcttc cttaatcttg
      781 tttttgtaaa tcaattcatc ttgtttggat gatttctttg actttttcgg cacagcaggt
      841 tgtatttttg acgacatcgt tactccaaaa acgtaaaacc gtccagccct cggctttcag
      901 acggcctgtt acttctatat ccctttggat attgcgctca attttggcaa tccaaaactc
      961 acgatttccc tttattaccg cttttttctg ttcccaatcc ttaccgtgcc agaattcccc
     1021 atcgacaaat acggcaactt tatatttttt aaatgaaaaa tcaggttttc caaaaatgct
     1081 tccgctattt ttccgatacc tgagccccaa agcccacatt gccttcgcca acacaagctc
     1141 gggtttcgtc cctgtacttt tgttggactg catacatttt ttgcgctgct ccggggttaa
     1201 tttatccatt gggtttattt gagatttatt caaactgctt caagaagttc aaatcgttat
     1261 cgaagaacaa gcgcaggtca ttgacgttgt agcgcaacat ggcaaagcgg tcgagaccaa
     1321 taccgaaagc gaaaccggta tatttttcag ggtcgatatt gacatttttc aacacattag
     1381 gatgtaccat accgcaaccg cctacttcca accatttgcc gttttctccc atgatgtcga
     1441 tttcggcaga aggctcggta aacgggaaga aagacggacg gaaacgcact tgtaggtcat
     1501 cgcgttcaaa gaagcgacgg ataaagtcag tgaacactgc ttttaagtcg gcaaaagtta
     1561 cgccctcttc tacccacaaa ccctcggcct gatggaacat aggcgagtgc gtggcatcgc
     1621 tgtccacacg gtaaacgcgg ccgggggcga taatgcggat gggcggctct tttttatcga
     1681 gcatatagcg gatttgaatc ggggaagtgt gcgtacgcaa aacatcgccg ttttcaacgt
     1741 aaaacgtatc ctgcatcgca cgggcaggat ggtttgcagg gatgttcagg gcttggaaat
     1801 tgtgaaaatc gtcttcgatt tcaggcccgt ccgccacttc gaaacccatt ccgtgaaaga
     1861 gttcgaccac acgttgcaag gtcagggtta cgggatgcag gctgccgcct tcctgagcgc
     1921 gtccgggcag ggtaatgtcg agggcttcgg cggcaagtcg ggcttgcagc ttggcttcgt
     1981 tgagggcatc gcgtttggca ttaaaagccg tctgaaaccg gtttttgcat tcattgatat
     2041 gcgcacctat ggttttgcgc tcttcaggcg acatttgccc caaagttttc agaagtccgg
     2101 tcaactcgcc ggttttgcca agataacggg ctttgatttg ttctagagcg ttgaagtctt
     2161 gcgcagcttc tactgcggca atgccttctg caacgatacg gtttacattt tccataatat
     2221 caagcctgtc aattaatgat atgccacctg aaacaacgag gccgcctgta tagcctgaca
     2281 tatcagactc ttcagacgac cttattttct tcacggccaa tccattggat aaattttctg
     2341 tattttaacg caaatttatt taaaaaaata gactttaaag aaagaaaatc aatcacatag
     2401 aatttccgca aacaaagaaa aaaggaagcc gcagcttcct tttcaatttt ttggattaag
     2461 cagccaaagc agctttggct ttttcaacca attgtgcaaa agcggcttta tcgaacacgg
     2521 ccaaatcagc caatactttg cggtcgattt cgatagaggc gcgtttcaga ccgttcataa
     2581 atttgctgta agacaacccg ttttcacgcg cacctgcatt gatacgaaca atccacaatt
     2641 gacggaattg gcgtttgcgt tggcggcggt cacggtatgc gtattgacca gctttcatta
     2701 ccgcctgctt ggcaacgcgg taaacgtttt tacgacgacc acgatagcct ttggctaacg
     2761 cgaagatttt ttggtgacgg gcacgggcgg taacaccgcg ttttacgcgt ggcatattct
     2821 aaactcctta agcgtagggt aacattttag caacagaagc caaatcgcga tcatttacca
     2881 tagaggtacc gcgcagttgg cgtttgtttt tggtggtctt tttagtcaag atgtggcgtt
     2941 tgaacgcatg agcgcgtttc acaccgccgt tacccagtac tttaaagcgt tttttcgcgc
     3001 tagacttggt tttcatttta ggcatgggaa aactccattc gttatcggat aaggcattag
     3061 ggggttttaa aacatcggtt tcaaacactt gaacccacaa tgccacggtt ttttgccgca
     3121 attgaaaact tactccgaag cggcaatcgg agtaagcgga aaattatagc tttatttttt
     3181 cttcggcgca atcatcatca ccatttgacg gccttccatt ttgggaaagg actcgatttg
     3241 cgccacttca gccaaatctt cttttacacg ttccaaaagt tgcgcgccga gttgctggtg
     3301 agccatttca cggccgcgga aacgcaatgt cactttgact ttatcgccgt cggcaaggaa
     3361 gcggttgatg ttgcgcatct tgatttgata atcgccctca tctgtacccg gacggaattt
     3421 gatttccttg atttgcacct gcttttggtt tttcttggct tcgtcgcgct tcttggcttg
     3481 ttggtatttg tatttaccgt aatccatcag tttgcacaca ggaggtttag cagttgggga
     3541 aatctctacc aaatcgacat cctgcccttc ggccatagcc aaagcttcac gaactgaaac
     3601 gacaccaagc tgttcgcctg actcactgat taaacgcact tctttggcgg taatttcacc
     3661 gttgattcgt gcttcgcgtt cttgagcgat gatgaatact cctataaaaa ttaatgattg
     3721 acgagggcat cagtgatttc ttgctgcaat tgcgcaatga aatcatccaa atccaaagaa
     3781 cccaagtctt ccgctttgcg gcgtaccgca actttgtttt cttgtttctc tttatcgccg
     3841 ataacgattt gataagggaa acggtattgg ctgttgtcgc ggattttgta accgattttt
     3901 tcattacgca aatccaactc ggcgcggaag cctgccgcct gcaatttggc agcaacttcc
     3961 cgacaataat ctgcctgatt ttcggtgatg ttcataatca ccatttgaac cggtgcaagc
     4021 cataacggga aagagcctgc atggttctca atcagaatgc cgacaaaccg ctccaaagaa
     4081 cctaaaatgg cgcgatgcaa cataacaggc cgcgctcggt cgttgttttc tgttacatat
     4141 tctgcatcca agcgttccgg caagacgaaa tccagttgta atgtaccgca ttgccaagaa
     4201 cgacccaagg catctttgac gtgatattcg attttaggcc cgtaaaacgc accctcgccc
     4261 ggcaattcgc cccattccac gccgcaggca gtcaatgcct cgcgcaaacc ctgctctgcc
     4321 ttatcccaca cgtcgtctga acctgcgcgt ttttcagggc gaagggaaag cctgacggat
     4381 acatcatgga aaccgaactg tttgtagatg cgaaccaaca attcattgaa cgcacgagcc
     4441 tcgctgacga tttgatcttc ggtacaaaaa atatgcgcat catcctgcac aaaaccgcga
     4501 accctcatca gaccgtgcag tgcgccgctc ggctcgttgc ggtggcaaga accgaattcc
     4561 gccaaacgca tcggcaaatc tcgatatgaa cgcaaaccgt tgttgaaaat ttgaacatga
     4621 cccggacagt tcatcggttt aaccgcatat tcgcgttttt ccgaactggt tacgaacata
     4681 ttgtctttgt agttgtccca gtggccggat ttttcccaaa aggttttatc cataatttga
     4741 ggcgttttga cctctttata accggcggcg ttcagctctt tacgcatatg ctgctcgata
     4801 gtctgccaca aagcccaacc tttgggatgc caaaacacca tacccggcgc ttcgtcctgc
     4861 aagtggaaca aatccagttg cttgcccaat ttgcggtggt cacgtttttc tgcttcttca
     4921 atgcgttgga tatagtcttt taattcgtct ttcgtcgccc aagcagtgcc ataaatacgt
     4981 tgcagcattt cattattgct gtcgccgcgc caatatgcgc ctgccagctt ggtcagcttg
     5041 aagtttttca ggaagcgggt gttcggaacg tgcggaccgc ggcacatatc gacatattcc
     5101 tggtgatgat atatccccat cgcttccact tcgggcatat cttcaatcag gcgcagtttg
     5161 tattcttcgc cgcgctcttg aaaaatttta atcgcctccg cacgcggagt catgattttg
     5221 accacatcat aatcttgggc aatcaattct ttcatacgcg cttcaatggc ggcaacatct
     5281 tccggtgtaa acggtttttc cgtggcgatg tcgtaataaa agccctcttc aatgacgggg
     5341 ccgataacca tttttgcatt aggatagagt tgcttgacgg catgcccgac aagatgtgcg
     5401 caggaatggc ggatgatttc gatgccttcc tgatctttcg gagtaatgat ttgaacagca
     5461 gaatcctcaa caatcgggtc gcacgcatcg accaatttgc cgtttacctt gcctgccacc
     5521 gccgccttcg ccaaaccggc accgatagac gcagcaattt gagccacggt aacgggggat
     5581 tcgtattggc ggactgaaca gtccggcaag gtaatattca acatcaaacg ctccgaaaga
     5641 ttaaaaaata acggcataaa tgccgttatg ggaatttggt aggcacgatt ggattcgaac
     5701 caacgacccc caccatgtca aggtggtgct ctaaccaact gagctacgtg ccttcaaaga
     5761 atttttgtat tttatcgggt cgttttaatt tttgcaagag agatgtgcat ttctttatgc
     5821 aacattgtgg caagatttgg ggataccgtc tgaaaaaccg tgggaattca tttataatgc
     5881 cgtcttttaa aatatatttc gacccctgtc gcccaactta cattcaatct tatgcttaaa
     5941 tttactttac acaaaaaaga cggtctctcc cgacgcggca cgctggagtt gaaccacgga
     6001 aaaatcgaaa cgccggtttt catgccggtc ggtacttacg gttcggtcaa agcgatgaac
     6061 ccgcaaaacc tgcacgacat caaggcgcaa attattttgg gcaacactta tcatttgtgg
     6121 ctgcgtccgg gtttggaggt cgtcgaacag tttggagggc ttcacggttt tatcggctgg
     6181 gacaaaccga ttctgaccga ctcgggcggt tttcaggttt tttctttgtc ggatatgcgc
     6241 aagctgaccg aagaaggctg tacgtttaaa agcccgatta acggcgacaa gctgttttta
     6301 tcacctgaaa tttccatgaa aatccaaacg gttttgaact cggatatcgc gatgcagttg
     6361 gacgaatgca cgccgggcga aacaacgcgc gaacaggcgc gaaaatccct gcaaatgagc
     6421 ttgcgctggg cggaacggag caaaaaagcc tttgaagatt tgaaaaaccc gaacgcgctg
     6481 ttcggcatcg tgcaaggcgc gatgtatgag gatttgcgcg aagaatcttt gcgcggtttg
     6541 gaggaatttg attttcccgg ccttgccgtc ggcggtttgt ccgtcggcga acccaagccc
     6601 gaaatgtacc gtatgctgca cgccgtcggc ccgattttgc cggaacacaa gccgcattac
     6661 ctgatgggcg taggcacgcc ggaagacctc gtgtacggcg tggcgcacgg tatcgatatg
     6721 ttcgactgcg tgatgcccac ccgcaatgcg cgcaacggct ggctgtttac ccgtttcggc
     6781 gatttgaaaa tcaaaaacgc caaacacaag ctcgacaagc gtccgataga cgaaagctgc
     6841 acctgctacg cctgccaaaa tttcagccgc gcctacctgc accatctgca ccgcgccggc
     6901 gaaatcttgg gcgcgcaatt gaacaccatc cacaacctgc atttctacca agtcattatg
     6961 gcggaaatgc gcgaagccgt cgaacaaggc aaatttgccg actggcaggc gcaattccac
     7021 gaaaaccgtg cgcgcggcgt ggactgattc ccggcaatgc cgtctgaagc cgcttcagac
     7081 ggcattttgc cgccttaccc tacctgcact atttttcaga aaccgatcat gcttccattc
     7141 cttccagaac cctccctttc ctatacgcaa caaaaccgta ccgccgtttt gctgttgaac
     7201 ctcggcacac ccgacgcacc gaccgcacag gcggtacgcc cttatctgaa atcctttctg
     7261 accgaccggc gcatcgtcga actgcccaaa tggctgtggt atcccattct gcatggcctg
     7321 gtactgacgt tccgccccaa aaaaagcgcg cacgcctatg aaaaaatctg gtttaaggaa
     7381 ggttcgcccc tagaagttta cacagcacgc caagctgccg cgcttgccga acgtatgccc
     7441 gacctcatcg tccgccacgc catgacttac ggcaatcctt cgatagcgga tgtattggcg
     7501 gagctgaaat cccaaggtgt aggcagattg cttgcgattc ccctatatcc gcaatatgcc
     7561 gcttccagca gcggagcggc ggtagataaa atgtgcgaac aactgctgct gcaacgcaac
     7621 caaatgagcg tccgcaccat ttcgcgcttt tatgatgatg ccggctacat tgatgcgatg
     7681 aaaaaccaca tcctccgata ttgggcggaa cacgggcgtg ggaaaaaact gatgttgagt
     7741 tttcacggcg taccgcagaa gcactacgac ctcggcgacc cctatcccga cgagtgccgc
     7801 cacaccgcca aactgcttgc cgaagcactg gaactgaccg aagacgaata taccgtatcg
     7861 ttccaaagcc aattcggcag ggcaaaatgg gttacaccga gcacgcagga tttgttcggc
     7921 aaactgccca aacagggggt aaccgagctg gacgtattct gcccgggctt tttggcagac
     7981 tgcttggaaa ccatggaaga aatcgccctg acggggcggg aacagtttta tgaagcgggc
     8041 ggaaaaaact accgctacat cccctgcctc aacgacaacc ccgactggat agacgcactc
     8101 gtcgcacttg ccgaagaaaa ccttggcggc tggcgttgaa actgtttgag ccatgtctaa
     8161 aatgccgtct gaacgccgtg ttcagacggc attttcccgc tactttagcg tatgatggca
     8221 tctatgccgg acagcacatc aagtgcaacc ttactaccga ttcggcaaaa aaacctttca
     8281 cccgctacgc aaaatcaaca acaaggtttc aaacattaag gaaacgccaa tgaacacaac
     8341 ccgactgccg accgccctcg tcttgcactt cctctgcgcc gccgcttctg ccgccgacaa
     8401 cagcatcatg acaaaagggc aaaaagtgta cgaatccaac tgcgtcgcct gccacggcaa
     8461 aaagggcgaa ggccgcggaa ccatgtttcc gccgctctac cgctccgact tcatcatgaa
     8521 aaaaccgcac gtcctgctgc acagcatggt caaaggcatc aacggtacaa tcaaagtcaa
     8581 cggcaaaacc tacaacggat tcatgcccgc aaccgccatc agcgatgcgg acattgccgc
     8641 cgtcgccact tatatcatga acgcctttga caacggcggc ggaagcgtta ccgaaaaaga
     8701 cgtaaaacag gcaaaaggca aaaaaaaacc aaacagacaa aatgccgtcc gaaaccggca
     8761 atccggcttc agacggcatt caaatcaaac ctttctaacg cggtactttc agccccatag
     8821 acccgatatt gccgccaccg tcaacctcct tcgcggtcag cataaagccg ccgatgtcaa
     8881 tacggtcgcc ctcgaccggc tcctgactgt cggaacgctt atcgaaaagc tcggcaaggc
     8941 tcaaaccctc ttcgcccgct tccagcttca aaccgtaagc aagtgccaaa tcgcccgaac
     9001 gtgctgccgg cgaaacgaca aactcaccga agaaatcaaa attttcacgg acgccgatac
     9061 ccgtctcggt aaagtatttc gccattttat cgaccttgcc gtcgggcagg atgtaccacg
     9121 ccaaatcctc cgcctgaagc acggtgtcgg actgcatttc gattcggctg ccgttgcgta
     9181 tcaatgcgaa acagcgtgcg tcaaacgaat cggaaatcgg ttcgaccgta tcgggatggt
     9241 gtccctccgc ttcagattcg gcaaccactt taaacgccga cattctgaca atttcctttt
     9301 ccgccagcca aatatcatgc gtatctttcg gttcgggctt gttgggcatg gcaaccttca
     9361 acagccgcgc catcacagga atcgtcgttc cctgaatcag cagcgacagc accaccacgg
     9421 caaacgccac atcaaacagc aggtgcgaat tgggaacgcc catcaccagc ggcatcatcg
     9481 ccagcgaaat cggtacggct cctcgcaagc ccaaccaact gatatacgcc ttttcacgca
     9541 ggctgtaatt gaatttccac aaaccgccga acactgccag cggacgcgcg accagcatca
     9601 ggaacgccgc aatcgccaag gcttccgccg ccctgtccaa cacgccggcg ggagaaacca
     9661 gcagaccgag catgacgaac aaagttgcct gcgccagcca agccaaaccg tccatcacac
     9721 gcaaaacgtg ttccgtcgca cggttgcgct ggttaccgac aatgatgccg gcaaggtaaa
     9781 ccgccaaaaa gccgctgccg cctatggtat tggtaaacgc aaacacaagc agcccgcccg
     9841 acacaatcat cagcgcgtac agaccttccg caagattcag acggcgtacc agctttgcca
     9901 atatctttcc gcccgcccaa cccgtcagca gaccgaaacc gatttgcagc gcaagcatcc
     9961 ggacaaacgc tgccgcaccc gattccgccg gctgcataat catggtaatc agtgccgtaa
    10021 ccaaaaaaac cgccatcggg tcgttcgcac ccgattcgat ttccaaagtc gcctgcaccc
    10081 gttcgttcaa acgcacgccg ctgttgcgca aaaggctgaa taccgcgccg gcatcggtcg
    10141 aaccgacaat cgccgccatc agcacgccga acttccaatc caaaccgaga taaaaagttg
    10201 caaacagtcc cagcggaagc acagtggcaa acacgcccca cgaagcgagt accgacgcgg
    10261 gcttcaacgc aatccgaaaa ctggaaagct gcgtccgcaa accgccgtcg agcagaataa
    10321 ccgccaaagc aagctggctg atgaaattcg ccatcacgac attattgaag gcaatgccgc
    10381 caacgccttc ctcgcccgcc agcatgccga cggcaagaaa caccagcagc aaaggcattc
    10441 ccaaacgtgc ggacaaagtg gtcgaaacca cgctcaggaa caacagtgcg ccacccagca
    10501 gaaacaggct gttcatccaa tccacgccaa cggctccccc ttaaaattca tgaaattaga
    10561 atcgaaagat tgtaacacgg cattttcttc tcacgaaact ttatttcatg tccgccaccg
    10621 ccttcagacg gcattttcct gtttcatgcc gcctgatacc gcccaaaccc gcgttctcct
    10681 atataatgtc ggactttgcg ccgcatttca ggatttttta tggcatttgc ctcactattt
    10741 actctgccgg actacatcac cgccgttttg gacgatgttg ccctgatgac caaagccgcc
    10801 gcaaagaaaa ccgccggcgt ggccggcgac gatttggcac ccaatgccaa ccgggttaca
    10861 ggcgtatcgg cagaacgcga gttgccgatt gtttggccgg tggcgaaagg ttcttttgcc
    10921 aacaagccgg ttttggtgtc tgcggctctg ctgctttccg ccttcctgcc gcaactcatt
    10981 acgccgcttt tgacggcagg cgggatttat ttgtgctttg agggcgtgga aaagctgctg
    11041 cacaagtttc tgcaccgaca tgacgcgcac gatgacggcg gcgaaacggc ggttaacgag
    11101 caggctaaaa tcaaaggtgc aatccgtacc gactttatcc tgtccgcaga aatcatcatc
    11161 atcgcgctgt gcgtggtcga acaatacagc ctgatgacgc gctcgctcgt gatggcggca
    11221 atcggcatcg gcatgactgt tttggtgtac ggaatcgtcg ccgtcatcgt caaactcgac
    11281 gacttgggca tgctgctgat gcgccgccca caacctcggc cttttgcacg atttcctgca
    11341 cgcgcaacac tggaacgctg gctggacaga gtattccgcc aacctcgttg tcgggccgct
    11401 ttccggttcg attgcctgcg ccccgccttg tcgctgatga atcgtttcgg caggcattga
    11461 ttcctttttc aaataccgat gccgtctgaa agacgttcag acggcatatt ccgaacagac
    11521 agacgaatat gttttccaaa ctggacaaat actggcagca ccccgccctc tactggcctt
    11581 tgctcatcct ttttgccgcc gccaccccct tcgccttcgc accctactac cacttttggc
    11641 tgatgccctt gattttcggc gccttcgtcc gcctcatcga actgcgtccg cgttttgccg
    11701 cctcttccgc ctacctgttc ggtctgaccg catacacggc acagttctac tggatacaca
    11761 ctgccctgca cgacgtttcc ggcctgcccg acctctatgc cgtaccgctg accttcctgc
    11821 tgcccgccta ccttgccctt tatccggcac tgtgtttctg gctgtggaaa aaatttaccc
    11881 tgccccaggg cataaagacc ggtctggtac tgcccatcct gtggacgctg accgagtttg
    11941 cccgcgagcg tttcctgacc ggattcggct ggggcgcaat cggctactcc caaatcaccc
    12001 cggacagccc gctggcgggt ttcgccccct tgggcggcat ccacatggtt acactggcaa
    12061 ccgcctttct cggtgtctgg ctggttttgg cgatcgacaa caccgcacgt tcgggcaaac
    12121 gcctgcttcc ggccattctg attgccgccc tgcttgccgc aggctacacc gcccaacaaa
    12181 ccgacttcac ccgccccgac ggcagccgca gcaccgtcgc cctgcttcag ggcaacatcg
    12241 cccaaaccct caaatggcgt gaagaccaag tcatcccgac catacagaaa tattacgaac
    12301 aggtcggcaa aaccactgcc gacatcgtca tcctgcccga aaccgccatt cccatcatgc
    12361 gccaaaacct gccggaaaac atactggcgc aattcgccga acaggcgcaa aacaacggca
    12421 gcgcgctcgc cgtcggcatc agccaataca cttcggacgg caacggttac gaaaacgccg
    12481 tcatcaacct gaccggctat caggaaaaca atcaggacgg catcccttac tacgccaaaa
    12541 accacctcgt ccccttcggc gaatacaaac cgctgcccct cctgaccaca ccgctttaca
    12601 aaatgatgga catgcccctt tccgacttcc gcaaaggcgg cggcaagcaa tccgccatga
    12661 tgatgaaaaa ccaaaaaatc gccttcaaca tctgctacga agacggattc ggcgacgaac
    12721 tgattgccgc cgccaaagac gccacactgc ttgccaatgt cagcaatatg gcgtggtatg
    12781 gaaaatccaa cgccatgtac cagcacctcc aacaatcgca ggcgcgggct atggaactcg
    12841 gacgctatat ggtccgcgcc accaacaccg gcgcaaccgc catcatctcc cccaaaggca
    12901 acatcatcgc ccaagcccaa cccgatacgg aaaccgtatt agaaggacac atcaaaggct
    12961 acatcggcga aacgccctat atgaaaaccg gcagttcatg gtggttgatg ggcatattga
    13021 ccctagccgc actgattctt ttcatcttcc gaaacaaaga acactgatac ctgcacagtc
    13081 cgtccggacg ggctgtcttt tttcaaaccg gccgtttaca tacaaccacg tatgtaatat
    13141 aattctaaaa tcgaattgag ctatcaggtt tacaaaatca atcaaacttg ccttacaaaa
    13201 ttttcctgtc tttcaaacct tagcgtatat ctcctgccaa acatacaaaa tacaagccaa
    13261 gcagtgatat actgcatccc aacacaccaa accgccgata cgcagtttca gccgaaagga
    13321 aatgccccaa atgaataacg ctttcgcatt acccgccatc caaagcggca acggcagcct
    13381 cgaacaatac atccacaccg tcaacagcat cccgatgctg tcccaggaag aagaaacccg
    13441 cctcgccgaa cgccggataa agggcgacct caacgccgcc aaacaactca tcctgtccca
    13501 cctgcgcgtc gtcgtttcca tcgcgcgcgg ctatgacggc tacgggctga atcaggccga
    13561 cctgattcag gaaggaaata tcggtctgat gaaggcggtc aaacgctacg agcccggtcg
    13621 aggtgcacgc ctgttttcat ttgccgtgca ttggattaaa gcggaaattc acgaattcat
    13681 cctgaggaac tggcgccttg tccgcgttgc caccaccaaa ccgcaacgca agctgttttt
    13741 caacctgcgc agcatgcgta aaaaccttaa tgtcctgtct cccaaagagg cacaagacat
    13801 cgccgacgat ttgggtgtca aactttccga agttctggaa atggaacagc gcatgacggg
    13861 acacgacatc gccatcatgg cagacaacag cgacgacgag gacagtttcg cccccatcga
    13921 ctggcttgcc gaccacgatt ccgaaccgag ccgacaacta tcgaaacagg cgcattacgc
    13981 cctgcaaacc gaaggcctgc aaaacgcctt ggcgcaattg gacgacagga gccgccgcat
    14041 tgtggaaagc cgctggcttc aagacgacgg cggactgacg ctgcaccagc ttgccgccga
    14101 atacggcgta tctgccgagc gcatccgcca gattgaagcg aaagccatgc agaaactgcg
    14161 cggtttcctg accgaagaag ccgaagcggt ttgaaatata cacaataaaa tacccgacaa
    14221 aacaatttgt cgggtatttt atttggtaaa catcaatgcg gtttgtgcgt cgtgccgtac
    14281 acgattttat ccgcccaagc catcgcaatc gccaaaatca aaaagcagac atgcaccaga
    14341 cactgccaca tcagctgctt ttccgacaga ttggcggaat tgataaatgt ttggagcaaa
    14401 tggatggatg aataccgata atcgacatcg acagccttac cttcaatacc ggtgcattca
    14461 catggctcaa ccactcgggc cggtcaggat ggtcgtcgat acgcaaccgg gatacgaacg
    14521 actcataccc gccaatctgc accatggtca gcaaattcgc aatcataacc acatcaatca
    14581 gatttaaaac agcgagcatg atggcgtttt cgtccgacac atccaaattc atgaccagat
    14641 gcttcaacga tttcaaaaac ttataggcac aaatcgcccg tacaacagtc agccccgcat
    14701 aaatcggcac ttgcagccaa cggcttgcaa aaatcatttt ggcaaaaaac ccatagcctg
    14761 ttcgggtttt ggagattttg cttgatacat ttcccttcct tgcagcaaca aaagcgcgtg
    14821 attccaatct accgctgcga aatcttcaat ttttcacacc cgaaaacgct tgccgccata
    14881 cagatgtgat atattgattc cattttaacg aacttcggag aacgacatga gcagcggaaa
    14941 tatccgcatc cacagcatga ccggctttgc caacgcggcg gcagagtgcg gcagcaaacg
    15001 catcaacctc gacatccgcg ccgtcaacca ccgctttttg gacattcaaa tcaggatgcc
    15061 cgacgatttg cgccatttgg aaaacggcat ccgcgagcag atttcatccc atctggcgcg
    15121 cggcaaagtc gaatgcaaaa tccaaattca agatgcggaa aacggcaatc agtctttgga
    15181 actgaatcgc gttctggtcg gacagcttgc cgaaatcaac aaaaacctcc gcaaacatta
    15241 tgatttggca aaactgggcg ttgctgatat tttgcgcttt ccgggcgtat tggcaagcca
    15301 aaaggaaaat acggaagcat tggcgaaaac cattgtcgaa ctggccgaca aagcactaaa
    15361 agactttacc gccgcacgtc ggcgtgaagg gaaaaaattg ggcgaacacc tgttacaacg
    15421 cattaaaaac atggaagaaa tcatcgatgc tttgagcgaa atcttccccg cgctggtgga
    15481 agcgcataaa gaaaaaatcc gcacccgcct tgccgaagcc gtcggcagca tcgacaacga
    15541 caggctgcaa caggaatttg ccctctttat ccaaaaatcc gacatcgacg aagaattcag
    15601 ccgcctgcgc acccacattg ccgaagtgcg ccgtatcgtt accgaacaca aaggcagcag
    15661 cggcaaacgg ttggacttcc tgatgcagga actgaaccgc gaagccaaca ctttgggcag
    15721 caaatccatc gctaccgaat gcacccaagc ctcggtcgag ctgaaagtcc tgatcgaaca
    15781 gatgcgcgaa caggtgcaga atatcgaata gccctgcctt tctctccaaa catgacaatg
    15841 ccgtctgaaa cttttttcag acggcattct tgatcggctg aaaacctctg ttgcaagcaa
    15901 ttcaagattg ttctttcaca tccaccaacc tcgttccggc aaggaagtca tacagaaact
    15961 gccggtcggg attcagcagg gcgaaccccc acggcaaaat cagccaaacc aatgccgcgc
    16021 cggccgcgcc cttgggcgga atgccgagga agtggcgcaa tccggcataa gcaagcatag
    16081 gaatgaagac gatgaatatg cacgcccaaa taaagcgcag gcgcagcagg tgcaaagacg
    16141 gctgtatgcc gttaaggtcg cacaagccga ttttccatgt cctcatcgcc aaggtctgcc
    16201 cctgaccgtg ccagttggcg cggaaataaa gccaccatgc tcccattatc aggatactcg
    16261 ttaccaatgc agaaaccgcg atagaaacgg gattcagaaa aatggcggca ataccggcaa
    16321 gcaatgctgc caaacaggtc gccgcaccga ccagcagcat ctcatacagt atggcggcaa
    16381 agcggcgttt gagcggggcg gttttcagta tcatcatctt ttcaatcgga atatgccgtc
    16441 tgaaacgcca acagacttca gacggcattt gtgcaaagat gccttgcggc ggttaacggt
    16501 gcggacgttt ctgaccgcgc gactgttttg ccgccatttg attgatttcc tgacgtgtcg
    16561 ggcgtttttt gaacatagcg tgcgcctcgt ccaccgaaca tttttgcgac aggcagatca
    16621 gggtaatcgc atctttctgg tgttgtccgc cgctgtggta catacggcgc aggtttgcct
    16681 cgttgattct gcctttttcg ggatcgatgt cgaatttttt cagacgttcg ataaactggc
    16741 gttgctgtcg gcgggatgtc caaacaaagc cgcccgcagc ggcggcgacg atgacggcgg
    16801 caagcaggat ggcaagcggc caggaatcga caatcaatac gataatgagg cttaaaacgg
    16861 cggtggtcag taaaatcagg ccgcccaagc gcagtttggt cttattgtcc atagggttga
    16921 tgttccttta ttgaggtggt cgtgccgtct gaaatgtttt cggacggatt ggtgaatact
    16981 tttcaggatt aacggtattt tacagtattt tcggattttg gggattaccg ctttattccg
    17041 ccagcgacac gacaagctgt ttgaataccg tccatgcgtc gccgtcttcc gcgcctttga
    17101 tgattcggtc gatttgggcg caggttttga gcgcgtcaag caggcggacg acggaaatcc
    17161 gcttgaccgc aagcggtgcg agcgtctgtt tgtcgcccca aagcctgagg ctgttgcgga
    17221 cggattggat gctctgcccc tgtttcaggg cagcggcaag ccgaatcagc gtccgcacgt
    17281 cttcggcaac cgcccacaga agcagcaccg gttcttcgcc ttcttcctcc aacccgtcca
    17341 acagcctgca tacgcgcggg acatcgccct tcatccacgc gcctgccagt tggaacgcgt
    17401 cgaagcgggc aacattggca acggcggttt gcgcctcatc gatattgacg gcatgccctt
    17461 tcggatacag cagcgcgagc ttgtcgattt cctgacgtgc cgccaacaga ttgccttcca
    17521 cgcgctcggc aaacagtgcc aatgcgtctg cctcgatacc caaaccgatt ttgtccagcc
    17581 gtccgcgtat ccattggggc aaagccgccg caccgaccgg tttggcttcc catacttccc
    17641 cctttgccgc caatgcggca aaccatttgg actggagccg ggttttctcc agtttgggca
    17701 gcaaaaccag cgttaccgta tcttccggca gtcgggcggc aaaatcctgc aatgcctcgc
    17761 cgccgttttt gccgggtttt ccgttgggga tatgcaattc caacagtttc aaatcggcaa
    17821 acagacccgc actgcctgcg gtttgcagca gttcgttcca atcgaaagac gcgtcggcgg
    17881 tataggcttc gcggttgaga taaccctgct tcttcgccgc cgccctcaat gcgtccactg
    17941 cctcgatacg cagcagttct tcctcgccgt ggatgacgta cagaggtttc aaaggcgcgt
    18001 ccgtatcgat gcgtccgata tgtgccgcca cgtcattccg ccttcagaaa ggtcaggcgg
    18061 cggacaatct gttcggcaac atcctgccgc atttccgccc acagggtttc ttcttcttcc
    18121 tgtttgccca aaatttcgtt gtcggcataa tccaaaatgc ggcggacgga cacggtcatc
    18181 ggtttgccga ccggctcgcc gcgtttcaat acctgcgctt caaccgtcaa tatcaaaaga
    18241 tattcgttga tgactgccgc acgggtaacg gtataggttt ccttgttttg ggaaacgctg
    18301 tctatacgca gggtcatctg cgcgccggca gcatcgtcca ccctgcccga agcctgatac
    18361 agcgcggttt ccaaaggaaa ctgcaatgcc tgtccgcctt cgatgtgcca gctccggtag
    18421 gtcagcggcg gagaaatgcc gtctgcacct ttcaggtgga aaccgcacgc gcccagcatc
    18481 aaggctgcgg cagtaaggaa tattttgttc atcgcttgcc tttctcaaaa gcataaagtc
    18541 ggtagattat atcagcggcg tgttcagacg gcattgccgt ccagccaaat caggctcgcc
    18601 atacgccccg tcgctccgtc gcggcggtag gaaaagaaaa tatcccgttc caaaaccgta
    18661 caatgcgtgc cgccgtacac gccgcccacg ccttcgcgct tcagaatcag gcgggcgagc
    18721 gcgtagaggt cggcaaggaa tttgccgccg ccgatgggtt caaatgcagc agcggcttcg
    18781 ggcatggacg tgcagaacgc atcaaacacg tcctgcccga cttcaaacgc atccgcactg
    18841 atggcagggc cgagatacgc cataatttcg acaggcggaa ccttcatcgc ggcaatggtg
    18901 ttttgcaata cgccgcccgc caaaccgctc cagcccgcgt gtgcggcggc aaccgccgta
    18961 cctgccctgt cgcaaaatag aacgggcaga cagtctgcag tcatcacggc acagacggct
    19021 ttacccgtgt cgtccacgga agcgtcggca tcgggcgtgc cgtccaatgc ttcggcagca
    19081 ttgacgacaa tggtgctgtg gatttgattg agataggcaa cgggcaatcc gacctgctgt
    19141 tgcacgattt cgcggttgcg gcgcacggtt tcagggtcgt cgccgacgtg cgtaccgagg
    19201 ttcaaactct gatacgcacc ttggctcacg ccgccgttgc gcgtggtaat cagggttttc
    19261 acattggcgg gcgcgggcca atcggcggtc aggaagtttt tgcctttcgg ggcgagattt
    19321 agggtttctg tgatggtttt cattggatat tggaataggt gtgcggcgga aagggttgat
    19381 taactgcctt cggggcaaat ggatgcttgt tttttcgtca ttcccgcaca ggcgggaatt
    19441 cggagattcg ggattgtttt caaacgttcg ggtttggatt cttgccggtg cgggaatgat
    19501 gcccttatat actttctccg agctttatat gcttcaacag gggacggcac atcaagcacc
    19561 gcactgcgtg ttgcccgaac gaggctgccc tccgattaga ttctatcgct ataaacagac
    19621 gggtttcaac cgaaaaggaa tggggataaa gtccatttcc gacacctctc gggcgatgcc
    19681 gtctgaaaac caatctccac tttcagacgg cattgtttag gcgcggtcat tttgcaaatt
    19741 aaggttaagg cgtgtatgct tgccttccct taaaaacaaa cacgcaacgc attattatga
    19801 atatcctcag taaaatcagc agctttatcg gaaaaacatt ttccctctga acctcgctct
    19861 ttgccgccgc cgcttttttc gcgcccgaca ccttcaaatg ggcggggcct tatattcctt
    19921 ggctgttggg cattattatg ttcggtatgg gtttgacgct caaaccttcc gacttcgata
    19981 ttttgttcaa acatcccaaa gccgtcatca tcggcgtaat cgcacaattc gccattatgc
    20041 cggcaaccgc ctggctgccg tccaaactgt tgaacctgcc tgccgaaatc gcggtcggcg
    20101 tgattttggt cggctgctgc ccgggcggta cggcttccaa tgtgatgacc tatctggcgc
    20161 gcggcaatgt ggctttgtcg gttgccgtta cgtctgtttc caccctgatt tccccattgc
    20221 tgactcccgc catcttcctg atgcttgccg gtgaaatgct ggaaatccaa gcggccggta
    20281 tgttgatgtc catcgtcaaa atggttttgc tccccattgt tttgggtttg attgtccata
    20341 aggttttggg cagtaaaacc gaaaagccga ccgatgcgct gccgctggtt tccgttgccg
    20401 ccatcgtgct gattatcggc gcggtagtcg gggcaagcaa aggcaagatt atggaaagcg
    20461 gcctgctgat ttttgcggtt gtcgtactcc gcaacggcat cggctacctg ctcggcttct
    20521 ttgccgccaa atggaccggc ctgccttatg atgcacaaaa aacgctggcc atcgaagtcg
    20581 gtatgcaaaa ctcgggcctg gccgccgcgc ttgccgccgc acactttgcc gccgcgccgg
    20641 tcgttgccgt tccgggcgca ttgttcagcg tgtggcacaa tatctccggc tcgctgctgg
    20701 caacttattg ggcggccaaa gccggtaaac ataaaaaacc ctaaacgcct ttcatcataa
    20761 tgccgtctga acggggcaac cgaaaccggc ggtttctgcc cggccccttc agacggcctt
    20821 tgtttgaaat cggcggggac gattgcccgg ctgctccggt tcggctgcct gcgttcgccc
    20881 cgcctttcaa gccgcctcac tcccttacat aaaccacttc gacatcgtag tcgtcttcgt
    20941 tccaatcgtc atcgtcgtcc gcgccgaatt tgtcctgcca ttcctcttca ttgctcaaag
    21001 acgaatccaa accggcttca agacggagga cggacaacaa atgatatatg tcgtccggaa
    21061 tcggtgcctc aaacgaaacg gtttcgccgc tttccggatg ggtaaaactc aagcggtagg
    21121 cgtgcaacgc ctgacgcgcg cccaaacttt taacggcttc tttcaccgtg tcgccgcacg
    21181 gatggcgcgg gttgccgtaa accgggtcgc cggcaagcgg atggttggcc tcgcgcatat
    21241 ggacgcggat ttggtgcgtc ctgcccgttc cgagcgagca ttcgatgtag ctgtgggcaa
    21301 gatagcgttc caacactttg acgtgggtta cggcaggctt gccgccgaat ttgacggctg
    21361 ccattttcag gcggttgtgc ggatcgcgtc cgatttgggt ttcgatttta ccgtcgaagg
    21421 ggacgatgcc gttggcgacg gcgcggtaga tgcgtttgac cgtgcgttct tgaagctgcc
    21481 tcacgaggga attttgcgcc ggcagggttt tggcaaccac catcagcccg ctggtttctt
    21541 tgtccaaacg gtgtacgatg cccgcgcgcg gaatttggga cagctcgggg caatgcgcca
    21601 acaggccgtt gagcagcgtc cccgtccagt tgcccgccgc cggatgcacc accagtccgg
    21661 ccggtttgtt gacgacgatg acggtatcgt cttcgtaaac aatatccaaa tccataggct
    21721 ccggaacaaa cgccagattt tcctcactcg gacggacggt tacacaaatt tgctcgccgc
    21781 ctatcatttt gtctttgggt tgcgaaggtt tatcgtttac aataaccgcg ccttctttaa
    21841 tccatgatgt caggcggctg cgcgagtagt cgggcagaag tttcgccaac accgcatcca
    21901 accgcccgcc ggcaagctcg ggcggaacgg tcagaccgat acggttttca gttccggaag
    21961 ctgacgcaaa gtctgaatcg tcgctataat cggattcatt atcaaaggaa gtattctgca
    22021 tgaaaaaaat tcttttaacg gtttcattag gtttggcact gagtgcctgt gccactcaag
    22081 gtacggccga caaagatgcc cagattaccc aagattggag tgtggagaag ctctatgccg
    22141 aagcccagga cgaattgaac agcagcaatt atacgcgggc tgtcaagtta tacgaaatct
    22201 tggaatcgcg cttccccacc agccgccatg cccggcaatc ccaactggat accgcatacg
    22261 cctattataa agacgatgaa aaagacaagg ctctggcggc aatcgaacgc ttccgccgcc
    22321 tccatccgca gcatccgaac atggattacg cgctgtacct gcgcggcttg gtgctgttta
    22381 acgaagatca gtccttcctg aacaaactgg cctcgcaaga ctggtccgac cgcgacccga
    22441 aagccaaccg cgaagcgtac caggcgtttg cggaactcgt ccaacgcttc ccgaacagca
    22501 aatacgccgc cgatgcaacc gcacgcatgg tcaaactggt cgatgctttg ggcggcaatg
    22561 aaatgtcggt ggcgcgttac tacatgaaac gcggcgcata tatcgccgcc gccaaccgcg
    22621 ccaaaaaaat tatcggcagc tatcaaaata cacgctatgt cgaagaatca ctggccatat
    22681 tggaacttgc ctacaagaag ttggacaagc cccagcttgc cgccgatacg cgccgcgtat
    22741 tggaaaccaa cttcccgaaa agcccgtttt tgacgcacgc ttggcagccc gacgatatgc
    22801 cctggtggcg ttactggcat taagcccatc ctcgaaatgc cgtctgaagc cctttcagac
    22861 ggcattttcc gttccggcac cagccttccc gttcactcaa acggtttttt ctgccaatag
    22921 aatttataca ctctcaaacg ttgagccttt acgcctccgc gtcccaagcc gaattgcagc
    22981 gcgcccgaca aatccgtccg caacaacttg atgccgtgtg cgcggacacg gttctgcacc
    23041 gcttcggtcg gatgcttgta ggcgttggca taaccgcttg aagcaacggc atattcgggc
    23101 gaaacggcat tgaggaacac gcccgacgag gacgtattgc tgccgtgatg ccccaacacc
    23161 aacacctggc tgtacaggtt gcctccatac ttgccgacca ggctttcctc gcccttcgta
    23221 tccaaatcgc ccgttaccag cagtgccgca ccgcccgcca caacacgcaa aacacaactt
    23281 ttcccattat catcgatatt tttgcgttca gacggcctca aaaactcgaa atccacgccg
    23341 tcccattgcc aacgctgttc cgcacaatgc cgcgccccct cataaaattc cggctgtccg
    23401 gcataaatcc cgccgttcgg tatcttgccg actgcctgaa agccgccgtc gtggtcgctg
    23461 tcgtgatgcg acagaaccag cttgtccaaa cggcggacac ccgccgcatt caaactcggc
    23521 acgatacccg tttgtgccgc cgctaccgtt cccgtatcga aaagcagatg gcggtttgcc
    23581 gtccgcacaa gcaccgacaa gccctgcccc gcatcccaaa ccgtaaccgc agcttcgttt
    23641 tcaggtactg cttcaggacg gtaggataca aaccctgcca acagcaacac cgcccacgga
    23701 cgcaaaccca agccgcgcgg cagcagcaac agcaaagcgg cacacaccgc caacaccagc
    23761 agcggcaaag gtgcggcggc aacggcaaac tcaggcgaca catcggcaag ccacaccaaa
    23821 aaccgcaaag tgtattccgc caaaaatgcc cccgcctgct gaagcggcgc aaacggcacg
    23881 accgaaccca gcaacgccag cggggtcaac acccaagaaa accacggtat cgacaccgca
    23941 ttgaccaaag ggctgaccaa aggcagcgag gcaaacagat aaccgagcaa aaccagagac
    24001 aacaccgaag ccgcccactg cccgcgcacg gcggtttgcc gtttcccctc gtacaaacgc
    24061 cctgcacacg cccatatcag ggccgccacc aaaccgaaag acagccaagt accgacaccc
    24121 aaaaccgcca aagggtcgaa cagcagcacc gccgccaacg cctgccacca cgtcgcccac
    24181 gccgacagtc ttcccctgcg ccacgcccac gcgaacgccg ccaacatcaa aacactgcgc
    24241 tgcgtcggca cggaaaaacc ggcaagcagc gcgtaaaaca gcgcgcccgc acaccccgcc
    24301 gccaaaaccc acgcgcgcgg ccgggcggga aggcgcgggg aacaggcaag cagccgcttc
    24361 gccagccacg caaacagcac cgccaccatc gtaacgtgca aacccgagat actgaccaaa
    24421 tgcgtcagcc ccaacggccg gaacgcctgc cacaatccgg ggcgcaatgc cgactgttcg
    24481 cccacgctca acgcgcgcat cagcccgatg ccgtctgaaa gcccgccgtc cgcatccgcc
    24541 tgccgccaat tgcggctgat gcggctgcgc caaaccgcaa tcccccaacc gcttccgcca
    24601 tgcagcaaaa ccctgtccgc accgaccgtc cccacgccgc ctaccccgtt ggataatgcc
    24661 cacgcctcac ggttcaaacc gcgcaggttc aactccccga cgacggggtg cacgcgtgcc
    24721 gttatccgcc atctgctccc gaccgcccat tcgcgccgtt tgtagtccga cagcagcaaa
    24781 tcaaacgtcc gaccgccgct gtccacagcc tttgccgcaa actgcacgcg ccgcccgtcc
    24841 gacctcggca tatccgtcac ttccaccgtc aaaggcacgc ctgaaaccgc ctccgcccgc
    24901 cattgcgaag acagtgccgc ttccgttctg aatacgccgt aagccgcgcc cgccaacacg
    24961 cacagcatca gaccggcaaa cgcaaaacgc cttgcaagca cagccaaaac cgcaaaagcc
    25021 gccaaccaaa acggccaatg cggtacaacc gacagtgcga acgaaactgc cacgccgaca
    25081 acccaacacg gcaaaaacca acgacggaac acgtctcccc actcccgata taaaaaacaa
    25141 acgatgataa agcaaagcca taaaaccgtc ataacaagat ttgaaaatga taagaatgca
    25201 aacgaaaagc ctcagtatta tccgagacaa cggcaaactt tccatcgtca ttcccgcaaa
    25261 agcgggaatc caaagacaaa aagccgcaga aatttatcgg aaagaaccga accacaaaaa
    25321 ccggcttccc accttcgctt gaataacaga acaaagaaag cggaaataaa aacatcagga
    25381 ttctttttta aattggcaat aaaacaccaa aatgaatgag ttacacaaaa aaatactcaa
    25441 caccacccaa ccggcgtaaa atgcaaaaca ttatcgctat taaaacggta aaaccttatg
    25501 aaagccaaac gttttaaaat taacgccata tccttatcca tctttcttgc ctatgccctt
    25561 acgccatact cagaagcggc attggtgaga gacgatgtcg attatcaaat attccgtgac
    25621 tttgcagaaa acaaaggcaa attttttgtc ggcgcaaccg atttatcagt gaaaaacaaa
    25681 cgaggtcaaa acatcggcaa cgcattatcc aacgtaccga tgattgattt tagcgttgca
    25741 gatgtcaaca aacgcatagc aacggtagtc gatccccaat atgccgtcag cgtcaaacac
    25801 gccaaagcag aagtccatac tttttattac ggccaataca acggtcataa tgatgtagcc
    25861 gacaaagaaa atgaataccg cgttgtcgaa caaaataact atgaacccca taaagcttgg
    25921 agtgcgagca acttaggccg cctcgaagat tataatatgg cgcgttttaa taagtttgta
    25981 accgaagttg cgccgattgc accaactgat gccggcggcg gattggatac ctacaaagat
    26041 aaaaaccgtt tttcctcttt tgtcagaatc ggtgcgggca ggcaattagt ttatgaaaaa
    26101 ggggtttatc atcaagaagg aaatgaaaaa ggctacgatt tgcgcgatct ttcacaagcc
    26161 tatcgttatg ccattgcagg tacgccttat aaagatatta atattgacca aacaatgaat
    26221 accgaaggtt tgatcggctt tggtaatcat aataaacagt attcggcaga agaacttaaa
    26281 caagcacttt cgcaagatgc gttaacaaat tacggcgtgt tgggcgatag cggctctccg
    26341 ttatttgctt ttgacaaaca aaaaaatcaa tgggtctttt tgggaactta cgattattgg
    26401 gcaggttacg gaaaaaaatc atggcaagaa tggaatatct ataaaaaaga atttgcagat
    26461 aaaatcaaac aacacgataa cgccggcacc gtcaaaggta atggagaaca tcattggaaa
    26521 accacgggta caaacagcca tatcggttcg acagcggtaa ggcttgccaa caatgaagga
    26581 gatgcgaaca acggacaaaa tgttaccttt gaagacaacg gcactttggt attggatcaa
    26641 aacatcaacc aaggcgcggg cggcctgttt ttcaaaggcg attacacagt caaaggcgca
    26701 aataatgaca tcacttggtt aggtgcgggg attgatgttg ccgacggcaa aaaagtcgtt
    26761 tggcaagtca aaaatccgaa tggcgacaga ttggcaaaaa tcggcaaagg cactttggaa
    26821 ataaacggca caggcgttaa ccaagggcaa ttaaaggtcg gcgacggtac ggttattctg
    26881 aatcaaaaag ccgatgccga caaaaaagtt caggctttct cccaagtcgg cattgtcagc
    26941 ggacgcggta cattggtatt aaatagttca aatcagatta atcccgataa cctatatttc
    27001 ggtttccgtg gcggtcgttt ggatgccaat ggcaatgact tgacttttga acacatccgc
    27061 aacgtggatg aaggcgcgcg cattgtcaac cacaacacag accacgcctc cacaatcaca
    27121 ctaacgggta aatctttaat taccaatccg aacagccttt cggtacatag catacaaaat
    27181 gactatgatg aagatgatta tagttattat taccgaccaa ggcgacccat cccacaaggc
    27241 aaagatcttt attacaaaaa ctatcgttat tacgccctaa aatccggcgg cagcgtgaac
    27301 gcaccgatgc ccgagaacgg tgtagcagaa aataacgact gggtctttat gggatatacg
    27361 caagaagaag ccaagaaaaa cgcgatgaac cacaaaaaca atcagcgtat tagcggtttt
    27421 agcggtttct ttggcgaaga aaacggaaaa ggacataacg gcgcattaaa ccttaatttc
    27481 aatggcaaaa gcgcgcaaaa ccgtttcttg ttaacaggcg gcgccaattt aaacggaaaa
    27541 ataagcgtaa ctcaaggcaa tgtcttgtta tcaggtcgtc caacaccaca cgcaagagat
    27601 tttgtgaaca aatcttcagc ccgaaaagac gcacatttct ccaaaaacaa tgaagtcgta
    27661 tttgaagacg actggataaa ccgcacattc aaagccgcag aaattgcggt taaccaatcc
    27721 gcatcattct cttccggaag aaatgtatcc gacatcaccg ccaacatcac cgcaacagac
    27781 aacgccaaag taaatttggg ttacaaaaac ggcgatgagg tttgcgtgcg ctcggactat
    27841 accggttacg ttacctgcaa cacaggcaac ttatccgata aggctttaaa cagctttgat
    27901 gcgacacgga ttaacggcaa tgtgaatttg aatcagaatg cggcattggt cttgggcaag
    27961 gctgcattat gggggaaaat tcaagggcaa ggaaacagcc gtgtcagcct aaaccaacat
    28021 agcaaatggc atttgaccgg cgacagccaa gtacacaatc tgtcattggc ggatagccat
    28081 attcatttga acaacgcttc cgatgcgcaa agtgcaaata aataccacac gatcaaaatc
    28141 aatcatttat ccggtaacgg gcattttcat tatctgacgg acttggcgaa aaatcttggg
    28201 gataaagtgc ttgtgaagga atccgcatcc ggccattatc agctccatgt tcaaaataaa
    28261 acaggcgaac ctaatcagga agggctggat ctctttgatg catcatccgt acaagaccgc
    28321 tcccgccttt ttgtttcctt ggcaaatcat tacgtcgatt taggcgcatt gcgttataca
    28381 atcaaaacag aaaacggtat tacccggttg tacaatcctt atgccgggaa ccgccgccca
    28441 gtcaagccgg ccccgtctcc tgccgcaaac acggcttctc aagcacaaaa ggcaacacaa
    28501 acggacggtg cacaaattgc caaacctcaa gatattgtcg tcgcaccgcc cagcccgcag
    28561 gcaaatcaag ccgaagaagc aaaacgccaa caagcaaaag cggagcaagt gaagcgtcag
    28621 caagcagaag cagggcgtaa atcggcagag ttgtcggcaa aacaaagggc cggggaagag
    28681 gaac
//
LOCUS       DAXDSD010000015        26077 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXDSD010000015 DAXDSD010000000
VERSION     DAXDSD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26077
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.015"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(85..2127)
                     /gene="recG"
                     /locus_tag="ACFB2Q_000509"
     CDS             complement(85..2127)
                     /gene="recG"
                     /locus_tag="ACFB2Q_000509"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC0917605.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLGRLKLPRLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAGESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2213..2656)
                     /gene="secB"
                     /locus_tag="ACFB2Q_000510"
     CDS             complement(2213..2656)
                     /gene="secB"
                     /locus_tag="ACFB2Q_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC0917606.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2677..2934)
                     /gene="grxC"
                     /locus_tag="ACFB2Q_000511"
     CDS             complement(2677..2934)
                     /gene="grxC"
                     /locus_tag="ACFB2Q_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC0917607.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3153..4646
                     /gene="rng"
                     /locus_tag="ACFB2Q_000512"
     CDS             3153..4646
                     /gene="rng"
                     /locus_tag="ACFB2Q_000512"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC0917608.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4681..5931)
                     /locus_tag="ACFB2Q_000513"
     CDS             complement(4681..5931)
                     /locus_tag="ACFB2Q_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC0917609.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVGPDSKSET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLRADGDDDYYRAYIRTTEQGRIAV
                     MQENEYREDLAEDAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRING
                     LPALSVYNIPSEIRGFVTAINLLLKRVDEDIRRRQRFVADAAHELRTPMTALSLQAER
                     LNNMPLPPDAGRQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLVLEKRQDIGVAVGGDVEVSADETEIYTLVKTFADNAVRYTPNGGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESECARVLDPFYRILGTEQQGAGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(6013..6642)
                     /locus_tag="ACFB2Q_000514"
     CDS             complement(6013..6642)
                     /locus_tag="ACFB2Q_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC0917610.1"
                     /translation="MSASLKDGGYAVDWVKNGAQVAAAAAAQPYDLMLLDLGLPGRDG
                     LDVLSEIRAAGCTVPVLIVTARDDLYSRLNGLDGGADDYIVKPFDMAEFKARMRAVLR
                     RGSGQAQACLSNGALSLNPATYQVEIIAEGRQVALSNQEFSVLQALLARPGVILSRSD
                     SEDKVYGWGGEVESNAVDFLIHGLCKKLGKESIQNVRGVGWLMPRQDAV"
     gene            complement(6910..8223)
                     /locus_tag="ACFB2Q_000515"
     CDS             complement(6910..8223)
                     /locus_tag="ACFB2Q_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC0917611.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLSVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILISSIAAGIVPLP"
     gene            complement(8601..9173)
                     /locus_tag="ACFB2Q_000516"
     CDS             complement(8601..9173)
                     /locus_tag="ACFB2Q_000516"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC0917612.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            9332..10741
                     /gene="dacB"
                     /locus_tag="ACFB2Q_000517"
     CDS             9332..10741
                     /gene="dacB"
                     /locus_tag="ACFB2Q_000517"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC0917613.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNRWLLGGGRISDGIGIADTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(10806..11975)
                     /gene="metK"
                     /locus_tag="ACFB2Q_000518"
     CDS             complement(10806..11975)
                     /gene="metK"
                     /locus_tag="ACFB2Q_000518"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC0917614.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(12158..12268)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            12321..13214
                     /locus_tag="ACFB2Q_000519"
     CDS             12321..13214
                     /locus_tag="ACFB2Q_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC0917615.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(13259..14323)
                     /gene="tsaD"
                     /locus_tag="ACFB2Q_000520"
     CDS             complement(13259..14323)
                     /gene="tsaD"
                     /locus_tag="ACFB2Q_000520"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC0917616.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(14439..15626)
                     /gene="ccsB"
                     /locus_tag="ACFB2Q_000521"
     CDS             complement(14439..15626)
                     /gene="ccsB"
                     /locus_tag="ACFB2Q_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC0917617.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(15619..17571)
                     /locus_tag="ACFB2Q_000522"
     CDS             complement(15619..17571)
                     /locus_tag="ACFB2Q_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC0917618.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWRQDEARNRFLLHSMDAYTGLTEYSAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(17849..18472)
                     /locus_tag="ACFB2Q_000523"
     CDS             complement(17849..18472)
                     /locus_tag="ACFB2Q_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC0917619.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            18679..19308
                     /gene="yihA"
                     /locus_tag="ACFB2Q_000524"
     CDS             18679..19308
                     /gene="yihA"
                     /locus_tag="ACFB2Q_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC0917620.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(19429..21825)
                     /locus_tag="ACFB2Q_000525"
     CDS             complement(19429..21825)
                     /locus_tag="ACFB2Q_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC0917621.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLPQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            21977..23092
                     /gene="pilM"
                     /locus_tag="ACFB2Q_000526"
     CDS             21977..23092
                     /gene="pilM"
                     /locus_tag="ACFB2Q_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC0917622.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            23095..23694
                     /locus_tag="ACFB2Q_000527"
     CDS             23095..23694
                     /locus_tag="ACFB2Q_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC0917623.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSEIQKLKQEKDAFLIKKNKIGELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            23695..24342
                     /locus_tag="ACFB2Q_000528"
     CDS             23695..24342
                     /locus_tag="ACFB2Q_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC0917624.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            24360..24905
                     /locus_tag="ACFB2Q_000529"
     CDS             24360..24905
                     /locus_tag="ACFB2Q_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC0917625.1"
                     /translation="MKHYALLISFLALSACSQSSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            24924..>26077
                     /locus_tag="ACFB2Q_000530"
     CDS             24924..>26077
                     /locus_tag="ACFB2Q_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HFC0917626.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
                     SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ
                     HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS
                     AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI
                     VASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLA"
BASE COUNT         6618 a         7550 c         6257 g         5652 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta taaaaaatca gatttccgtt acactttttt
       61 ctaatatttc agacggcatt ttgctcacac acccaaataa ccttccctgc cggaaagcca
      121 ccttgccaaa tgcgcttcga cgatttcagg gttttgttca atcagcatcg gggcggtttc
      181 gcgcgcttgt tccaaaaggt gtaagtcttc ttcgagcttg gcgaagcgca gcataggcac
      241 gccgctttgg cgcgcgccga gaaattcgcc ggggccgcgg atattgaggt cttggcgggc
      301 gatttcgaag ccgtcggtgt gttcgtagat gactttcagc cgcgctttgg cgagttcgcc
      361 caagggttcg gcaaacagga ggacgcacac gctttccccc gcgccgcgcc ctacccgtcc
      421 gcgtaattgg tgaagctgcg ccaagcccat gcgctcggcg tgttcgatga ccatcagggc
      481 ggcattgggc acatctacgc cgacttcgat aacggtggtg gcgaccaaga cgttcagacg
      541 gcctgcggca aattccgcca tgacttcggc tttttcggcg gccttcatgc gcccgtgtac
      601 cagtccgata ttgggttcgg gcaatgccgt ctgaagccgg gcgagggttt cggcggcggt
      661 ttgcagttgc agggtttcgc tttcttcaat caatgggcag acccaatatg cctgccgccc
      721 ttttcggcaa gtgccgagga cgaagccttc gacttcggcg cggcggacgt tgttgacgag
      781 gcgcgtttta atcggagtgc gcccgggcgg caattcgtcg atgacggata cgtccaagtc
      841 agcgaagaaa ctcatggcga gcgtgcgcgg gatgggtgtg gcggacatca tcagctgatg
      901 gacttcgcgc cctttgtttt tgagtgcgag gcgttgtgca acgccgaaac ggtgctgttc
      961 gtccacaatg ctcaagccca aattgtgaaa cgccacgccg tctgaaaaca gggcgtgcgt
     1021 gccgacggcg attttgacgc tgccgtcggc gagtttggct ttggcttcgt ctttggcttt
     1081 tttgcgcaaa ctgccaaaaa ggcagacaac ttcaatgccc aaaggttcga gccattgttt
     1141 gaacttgatg aagtgctgtt cggcaaggat ttcagtgggc gccattacag ccacttgcgc
     1201 gcctgattcg atggcggtca gcgcggataa ggcggcgacg atggttttgc cgctgccgac
     1261 atcgccttgc agcaggcggt gcatggggtg ggtttgcgcc atatcgcggc agatttcgga
     1321 aacaactttt tcttgtgcat cggtcagggc aaacggcagg gcttggcgca gggcttgggt
     1381 caatgtgccg tcgccgcgca atgccgccgc cgtgccgctg acgcgcttct gccgcgccaa
     1441 acgcatggac aactgttgcg ccaaaagttc gtcgaatttg agccgctgcc acgccggcag
     1501 cgtgccgtct gaaagttgat gaatagtgaa actcggcggc ggcgaatgca aaaggcgcag
     1561 gctttcggca aggcgcggca gcttcagacg gcctaataaa gcatcaggca aggtgtcgtg
     1621 cagcggcgta acgtccaacg ccgtctgaat gatgcggcgc aaagtgggct ggttcaaacc
     1681 gtttacggtc gggtaaaccg gcgtgaggct ttccgccaaa ccgccgccct cggcatcgcg
     1741 gattttggga tgaatcatct cgtcgccgta aaatccgtgt ttgatttcgc ccacggcgcg
     1801 gatgcgtttg ccgaccgccg tctgcttctg atggctggcg taaaagtgga tgaagcgcag
     1861 aaaaaggacg ctgccggaac cgtcggcaat ttgaacaatc agctgcttgc gcggtttgaa
     1921 cgttacttcc tgatggataa cctcgccctc gacctgacac ggcacgccaa tcggcgcgtc
     1981 cttaatcggc ataatgtgcg tctcgtcctc gtaacgcagc ggcaggtgca acaccaaatc
     2041 ccacgcggta tggaggttga gtttgtcgag cttcttggcg gaaacatcgg tgattttgag
     2101 ctgtttctgg gtttcgggcg acatcatagg cagattcctt tggacgcgcc tattttatcc
     2161 gaaaacaaaa atgccgtctg aaacggattc agacggcatc ggcaggcagg aatcaagccc
     2221 cggcggcttc ggcttcctgc tgttgttggt aaatcgcttc aaaattaatc ggcgcaagca
     2281 ggacgggcgg gaagccggcg cgcgttaccg taccggaaac agcttcgcgc gcgtaaggga
     2341 agaggatgtt cggacacgcc acgcccaaca gcagctgcac atcttcttcg gggatgtttt
     2401 ccagacggaa aataccgctt tgggttactt cgttcaaaaa catcgtgcgc tcgttatcca
     2461 atttggcggt aacggttacg gtaacgtcca cgtcgtagta gccgtcttcc agcttttggc
     2521 tgccggtgga aacgcgcata tccacttcgg gatcgccctg ttccaaaaag atttgcggcg
     2581 cgtgcggcac ttccaaagac aagtctttga catacagtcg ctcgatgctg aatacgggtt
     2641 gcagttcttc gctcattttg ttttcctagt tgtgggttaa gggttcagca gtccgtccag
     2701 cccgccttcc tgctgaaggc ggtagaggtc ggtaaatcct ccgacgtgcg tttcgccgat
     2761 gaaaatctgc ggcacgctgc gctgtcccga aagccgctgc atttcggcaa aggcttcggg
     2821 gcttgcatcg acacggattt cgtcgatatg tccgacacct gccgcgtgca gcagcctttt
     2881 cgccatcgcg cagtaggggc aaaacggacc tgtgtacatg gtaacggtct gcatattggg
     2941 tttccgaaag ttttgcaatg ataatcaata taggggcaat cgctccgttt ggcaagtgcg
     3001 gaacagatgc acgttcagac ggcatgtgcg gagtgtgtca aagtttcttt tttaaagtat
     3061 gataggcatt gtgaaaaata tttttgcacc cgctctgcgc ggcggggcgg aacggatgca
     3121 aaaatatttt tattacattt tcaggaaaaa ccatgttgtc aggactcccc atccccaaag
     3181 acatcgcgcg cccgcccgaa acgatattgg tcaacatcac gccgcaggaa acgcgcgtgg
     3241 cggtgttgga ggaaaacaat atctgcgagc tgcacatcga gcgcaacagc gggcacagcc
     3301 tggtcggcaa tatctatctg ggcgtggtgc gccgcgtgct gcccgggatg cagagcgcgt
     3361 ttatcgacat cggcttggaa cgcgcggcgt ttttacacat cgtcgatgtc ctcgaacaac
     3421 gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct gtttgaaggg cagtcggttt
     3481 tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc gcggctttcc acccaaatct
     3541 cgctggcggg gcgtttcctc gtccaccttc cgcaagaaga ccacatcggc gtgtcccaac
     3601 gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg cctcgacaag ctcctgccgg
     3661 aaaatgcctg ccggggctac atcatccgca ccaacgccga aaacgccacc gacgaacagc
     3721 tccagtccga catcgactac ctgaccaaag tgtgggaaca catccaagaa caggcgaaaa
     3781 tccggccgcc ggaaaccctg ctttatcagg atttgccttt aagcctgcgc gtgttgcgcg
     3841 atatggtcgg ctgcgacacg cagaaaatcc tcgtcgattc caccgtaaac cacgggcgca
     3901 tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg caggatagaa ttgttcaaag
     3961 gcgaacgccc gctgtttgaa acccacaaca tcgaacagga aatcagccgc gccctgcaac
     4021 cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga gtccaccgaa gccatgacca
     4081 ccatcgatgt caacaccggc ggcttcgtcg gcgcgcgcaa tttcgacgaa accatcttcc
     4141 gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt aaggctgcgc aaccttggcg
     4201 gcatcatcat catcgacttc atcgatatgg cgcaggaaag ccaccgcgaa gccgtgttgc
     4261 aggagcttgc caaagccctc gccttcgacc gtacccgcgt taccctgcac ggttttacca
     4321 gcctggggct ggtcgagctg acgcgcaaac gctcgcgcga aaacttaaac caagtcctct
     4381 gcgaaccctg cccttcctgc caaggcaggg gccgcctgaa aacgccgcaa accgtatgct
     4441 acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta cgatgccgaa agtttccgca
     4501 tcctcgccgc ccccaacgtc atcgatttgt ttttggacga agaatcgcaa tccttggcaa
     4561 tgctgataga tttcatcggc aaaccgattt ctttggcggt cgaaaccgct tacacgcagg
     4621 aacaatacga tattgtgctg atgtaaaaaa tgccgtctga agccttcaga cggcatctgt
     4681 ctatttcagg gtttccttgt ccaacagcgc gcgtatcaac agcccgtgtc cgaaacgtcg
     4741 gctgtcggtc agttccaaat atccgccgta ttttttggcc agcgtgtcgg caatcgacag
     4801 ccccagcccc gccccctgct gctccgttcc caaaatacgg taaaacggat cgaggacgcg
     4861 ggcgcattcg gattcgggaa tgccgttccc gttatcctcc acccacacgg cgagatattt
     4921 cccttcgtcc gtgaaaccca aatctatcct gcccccgttc ggcgtgtaac gtaccgcgtt
     4981 gtcggcaaag gttttaacca gcgtatagat ttccgtttcg tcggcagaca cttcgacatc
     5041 gcctccgacc gccacaccga tgtcctgacg tttttccaaa accagcggca tcagttcctg
     5101 caacacttgg cggaaacggc tttgcagacc gaatgtcgtt ttcgtcaaag gggtttcgtc
     5161 cgactgcgaa cgcgccagtg ccaaaagctg ttcgagcagg tgtttgttgc gcctgatgct
     5221 ctgctgcaaa acggcggact gccgccccgc atcgggtggg agcggcatat tgttgagccg
     5281 ttccgcctga agggaaaggg cagtcatcgg cgtacgcaat tcgtgtgccg cgtcggcgac
     5341 aaaccgctga cggcggcgta tatcttcatc aacacgtttc aaaagcagat tgatggcggt
     5401 tacgaaccct ctgatttcac tgggaatatt gtacacactc aaagcaggca ggccgttgat
     5461 tcggcgttgt tcgagacttt gcgacaattt gcggacgggg cgcatggctt tgtgcgtaat
     5521 ccacacagtc agcagtatca tcagcggcag tgccgccaac aggggcaaca cgctttgccg
     5581 tgccgcatcc tccgccaaat cttcacggta ttcgttttcc tgcatgacgg caatccgtcc
     5641 ctgttcggtc gtgcggatat aggcgcggta ataatcgtcg tccccgtccg cccgaagcgt
     5701 gtgcagaccg tccgccagat gcgcgggcag gctgacaaca gggtcttcct gctgcggcat
     5761 ctgtaccaaa atacgcgtat cgccgtcgcc ttcgggcaaa gtttcggatt tggaatcagg
     5821 gccgacatac aatgccgcct gacggagcag gtcgtcctgc aacgcttccg tttcgtggaa
     5881 ggtttcgtag taggaaaaca tacctgcaag cattgccagc ggaacaaaca tccaaatcag
     5941 ggcaaggctg attctgacct gaagcgaatg ttttaaaatt ttaaaaaaac ggtccggcat
     6001 cttcgttcct gttcagacgg catcttgacg cggcatcagc cagccgacac cgcgcacatt
     6061 ttgtatgctt tccttaccca atttcttgca cagcccgtga atcagaaaat ccaccgcatt
     6121 gctttcgact tccccgcccc aaccgtaaac cttgtcctcc gaatccgagc gcgacaaaat
     6181 cacacccggc ctcgccagca aagcctgcaa taccgaaaac tcctggttgc tcaatgccac
     6241 ctgcctcccc tcggcaataa tttctacctg atacgttgca ggattgaggg ataacgcacc
     6301 atttgacaga cacgcctgcg cctgtccgct cccgcgccgc aacaccgccc gcatccgcgc
     6361 cttaaactcc gccatatcga acggcttgac gatatagtca tccgcaccgc cgtccaaacc
     6421 gttcagccgg ctgtacaaat catcccgcgc cgtaacgatc agcacgggga cggtacagcc
     6481 tgccgcgcgt atttccgaca aaacatccaa accgtcccgc ccaggcaaac ccaaatccag
     6541 cagcatcaag tcataaggct gggcagcggc agccgccgca acctgcgcgc cgtttttgac
     6601 ccaatccacc gcatagccgc cgtctttcaa acttgccgac accgcttacg caatcatcgc
     6661 attgtcttcc accagcaaaa cacgcatcaa ctttcccttc aaaataaacc gtgcctattc
     6721 taacacccca aaattagccg caatttagcg gcctttacgc ttgccggtat ttttcaaaac
     6781 tgcggcacaa aaaaaccgcg ccggcaactg ccttcagacg gcattggggc gcgattgcaa
     6841 cacacggaca ggcaggcaaa gcctgcgaca gatcacagga acgattcggg cttcaggcgg
     6901 cttcgccgtt tacggcagag gcacgattcc tgccgctatc gaactgatca atatcgccag
     6961 cgacaaaccc cacgcccaga aaaacgaata acggatgtgt ttgcccatcg acaatttcgc
     7021 caaacccaaa cccatccaca aagccggcga aagcggcgta acaaaagtac cgacgatact
     7081 gccgatcaac atcgcatagc ctgccgcttc gggtgcaacg cccgcctgcg aggtaatctg
     7141 ttccacaatc ggaaacagtc cgaaataata agcgtccgta ctcaaaacca actcaagcgg
     7201 aatacccaac acaccgatgg caatatgcag ataaggcagc aacgcgtccg gcaggatatg
     7261 cacaaggtct ttggaaatcg cgtccaacat ccccgcgccc ttcaaaatcc ccaaaaacgt
     7321 acctgccgcc aaaataatgg acgccatcat caccgcgccg ccggcgtggg cataaatccg
     7381 ctccatctgt tcctgcgggc tgcggtaatt caaaagcaac gccgccgttg cagccagcat
     7441 aaatacataa cccggcggga agatgcccga aaaaagcagg ctcatcgccg ccaaaaacag
     7501 caggacattc caccaaaaca gtttcggacg cgccaatttt tgttcttctt ccgacaaagg
     7561 cgccggcttt atcaaatccg ccacggcggg caacgcgccc aactcccgga caatccgcct
     7621 tttttcacgc acacccaaaa acagggacag cacaaggata aacaccacac cgataatttg
     7681 caccgtcaac aaaggtttat acaattcgcc cacatctgcg cccaacacgc ttgcaacccg
     7741 cccgatcggc ccgccccgcg gcaaaaggtt gattagcccc gcgctggaag tcagcagcaa
     7801 aaacagcagg taaggattca tatgcagacg cttgtaaagc ggcaaaaggg cggggacgac
     7861 cgataaaaac gtcgtcgcgc ccgccccgtc caactgtgcc accaccgaca ccaagaccgt
     7921 ccccacactc actgccacga tattaccccg agtcagctta atcaaaccgc ctatcatcgg
     7981 acggaacagc cccacatcgt tcatgattcc aaaaaacaaa atggaaaaca taaacataat
     8041 cacaatctgc gtcaccgatt tcgtgccgcc cgaataaaat tcttttaatt gggatacatc
     8101 aaaccccgcc agcaacgccc caatcagcgg caccaagatt aatgcgatga tgggcgacac
     8161 tttttccgtc agcaacagcc atacgatgac cccgataatc agcaatccga taaacgtcag
     8221 cattatttct cctttatttt attttgaaca taaaaccgcc cgtgcaggca aaaccgccaa
     8281 catatgcggg ataagccttg cattccattc acgcctgccg acatttctgc atccgtccgt
     8341 catttcacgg cggcaaccga tacggaacaa ccggcaaacc ggtatcggga cggcgcgggg
     8401 gcatccgtcc cggtgcaccg attcaaacga aactgccccc tatcattgcg gagcgcgggg
     8461 cgtgccgtac acgcgggatt ttaaacacgc cgatcatttg ccgcgcatta tccggcaaac
     8521 ggcaaacctt gacgctgccc ggcccatata aaaaagccgc aaaacccgaa ccggttttgc
     8581 ggcttttcga tttggtttgt ttatttggca tttctttcaa tcaaaccgac aaactgattg
     8641 atatatgact gaacaaaatc ccttgccgag tcaatcaact taccgttttc atcaaacagc
     8701 gtcggcgaat tgcccaaaaa tacttccggc tgtccggtta cgggcatatc aaaatacgac
     8761 agggcaaggc gcaggttttt ttgggaactg taaccgccca tcttgccgac ggaatggctg
     8821 atgatgccgg ccggtttgtt tttccaagcc acgtcggcat tcggtttcga gccgatgtcc
     8881 accgcatttt tcaaacaggc gggaatggtg cggttgtttt cggacgtaac gaacaaaatg
     8941 ccgtccgaag ccttaatcgt ttcgcggaaa gccgtgtagc tttcgggcag cggcacatct
     9001 tccaccgcag ggtcgtcata atcgaaattg taaagcggca gatgtccgat ttcgacgatt
     9061 tccgcctgcc agccttcggg gaacatctcc gccgcattca atgccacttt gcgcgcaaac
     9121 gaagcacggc gcaggctgcc caccaaaata ctgattttct tagccacaat cattcctcct
     9181 gaatattaag tttgtgcgtt tcaatcattt cataatggta acgattatta tatatgtgat
     9241 ttcccctgca aacaagccgg tccgccgccc cggcgttccc acttatccgg ctttgcctta
     9301 taattgcttt ttttatgtaa cagattcacc tatgaatttc cccaaaacag cggcctccct
     9361 gctgctgctt ctcgcctccc tcgccgcaca cgcgctcgat accggccgca ttccgcaaaa
     9421 cgaaatcgct gtatatgtcc aagagcttga cagcggaaaa gtcatcattg accaccgtgc
     9481 cggcataccc gtcaatcccg cgtccacgat gaagctcgtt accgcgtttg ccgccttcaa
     9541 aaccttcggc agcaattacc gctgggcgac cgagtttaaa agcaacggta cggtaaacga
     9601 cggcacgctt gacggaaacc tgtattgggc gggcagcggc gaccccgttt tcaatcagga
     9661 aaacctgctt gccgtccaac gccagttgcg cgacaaaggc atccgcaata tcacggggcg
     9721 cctgatgctc gaccacagcc tgtggggcga agtcggcagt cccgaccatt ttgaagccga
     9781 cagcggttcg ccgtttatga cgcccccaaa tccgactatg ctgtctgccg gtatggttat
     9841 ggtgcgcgcc gaacgcaatg ccgccggcag taccgacatc ctcaccgatc cgcctttgcc
     9901 gcatattttt gcccaaaaca acttgaaaat taccgcctcc caagctgcct gcccttcggt
     9961 caaaaaactg atgcgcgcat ctttttcggg caatacgctg aaattgcgcg gcaatattcc
    10021 cgaaagctgt ttgggcaagc ctgtcggtgt ccggatgttc gcgcttgacg aactgatccg
    10081 ccaaagtttt accaaccgct ggctgctcgg cggcggacgg atttcagacg gcatcggcat
    10141 agccgacaca ccggaaggcg cgcagacgct tgccgttgca cactccaaac cgatgaagga
    10201 aattttgacg gacatgaaca agcgttcgga caatctgatt gcgcgttccg tcttcctcaa
    10261 actcggcggc gacggcaaac tgcccgccgt ttccgaacag gcggcgtctg ccgtccggcg
    10321 agaacttgcc gtatcgggca tcgatgttgc ggatttggtt ttggaaaacg gttcgggtct
    10381 gtccagaaaa gaaagggtaa cggcgagaat gatggcgcaa atgttggaaa cggcttattt
    10441 cagcccgttt gcacaagatt tcatcgacac gctgcccatc gccggcacag acgggacttt
    10501 acgcaaccgc ttcaaacaaa gcggcgggct gttgcgctta aaaaccggca cgctcaacaa
    10561 tgtccgcgcc cttgcaggtt attggctggg cgacaaaccg atggcggtgg tcgtcatcat
    10621 caacagcggc cgcgccgttt ccctgctgcc cgacttggac aacttcgttg ccaaaaacat
    10681 catctccggc ggcgacggct ggctggatgc gaaactgatg tgcaaagaac gccgcgcctg
    10741 aatcgggaaa atataaaaat gccgtctgaa ctgttcggac ggcatttttg attttcaaac
    10801 cggaattaca gccccgctgc cgctttcaat gaggccgctt tgtcggtgcg ctcccaagta
    10861 aactcaggtt cttcgcggcc gaaatgtccg taggcggcag attttccgta aatcgggcgc
    10921 aagaggtcga gcatttggac gatgcctttg ggacgcaggt cgaaatgttc gcaaaccagc
    10981 gcaatcagtt tttcctcgct gattttaccc gtaccgaaag tatcgatgga aatcgaagtc
    11041 ggttcggcaa cgccgatggc gtaggaaact tggatttggc attgggttgc caagcctgcg
    11101 gcgacgatgt ttttcgccac ataacggcag gcgtaagcgg cggaacggtc cactttggac
    11161 gggtctttgc cggagaatgc gccgccgccg tgcggagccg cgccgccgta ggtatcgaca
    11221 atgattttgc ggccggtcaa accgcagtcg ccttgcgggc cgccgatgac gaagcggccg
    11281 gtcgggttga tcaggtattt tgtttcgtcg gtcagcagtt cgggcggcaa aacgggcttg
    11341 ataatctgct caatcacggc tttactcagt tcttcatggc tgatggcagg atcgtgctgg
    11401 gtggacagga cgacggtatc aatgcgtttc accttgccgg tttcgctgtc gtaaaccacg
    11461 gtcagttggg cttttgcgtc agggcgcagc caaggcaggc ggccgtcttt gcgcaattcg
    11521 ctttgacgct gcatcaggcg gtggctgtaa tagatggcaa acggcatcag ggtcggggtt
    11581 tcgtcgcagg catagccgaa catcaagcct tggtcgcccg cgccttggtt caggtcgatg
    11641 ccttcgcctt cgttcacgcc ttgggcgatg tcgggggatt gttggtcgta gtacacgccg
    11701 accgcgcagc cgttggcgtc aaagcccagc tcggaggagt tgtagccgat gcgtttgatg
    11761 gtttcgcgtg cgactttgat gtagtccact tgggcggtgg tggtgatttc gcccgccaat
    11821 acgcacaagc ctgtgttgac caaagtttcc gccgcgacac gcgctttggg gtcttgcgcc
    11881 aagatggcat ccaaaatcgc atcggatact tggtcggcta ctttgtccgg atggccttcg
    11941 gaaaccgatt cggaagtaaa cagatattcg ctcattgaaa ttttccttga aaaacaaacc
    12001 atcttcttca gacagcatgt tgtatgaaca taatgtcgac agcgggaaat atagcaaaat
    12061 ttccctattc gtgccattca gttgagaaat attcctattt aaacggcgct ttggaatctc
    12121 tgtccgtacg tttcgtacag gcaaaaaaat tcccgcatca agcgggtttg gattgctccg
    12181 gcgagccaca tcggtttttc aaccgtccac cttacctttc cgtttgaaaa gcaggttggc
    12241 atggaattcc caactcttaa tgcagcgcgc atcgtagcag aaaaggcata ttgccgcaat
    12301 acttcccttt ttcagacggc atgtttcgtt tacaattcag gctgtttccc cctttgcgaa
    12361 ccgccatgca catcctgttg accgccctgc tcaaatgcct ctccctgctg tcgctttcct
    12421 gtctgcacac gctgggaaac cggctcggac atctggcgtt ttacctttta aaggaagacc
    12481 gcgcgcgcat cgtcgccaat atgcggcagg cgggtttgaa ccccgacacg cagacggtca
    12541 aagccgtttt tgcggaaacg gcaaaatgcg gtttggaact tgcccccgcg tttttcaaaa
    12601 aaccggaaga catcgaaaca atgttcaaag cggtacacgg ctgggaacac gtgcagcagg
    12661 ctttggacaa gggcgaaggg ctgctgttca tcacgccgca catcggcagc tacgatttgg
    12721 gcggacgcta catcagccag cagcttccgt tccacctgac cgccatgtac aagccgccga
    12781 aaatcaaagc gatagacaaa atcatgcagg cgggcagggt gcgcggcaaa ggcaaaaccg
    12841 cgcccaccgg catacaaggg gtcaaacaaa tcatcaaggc cctgcgcgcg ggcgaggcaa
    12901 ccatcatcct gcccgaccac gtcccttctc cgcaggaagg cggcggcgtg tgggcggatt
    12961 ttttcggcaa acctgcatac accatgacac tggcggcaaa attggcacac gtcaaaggcg
    13021 tgaaaaccct gtttttctgc tgcgaacgcc tgcccgacgg acaaggcttc gtgttgcaca
    13081 tccgccccgt ccaaggggaa ttgaacggca acaaagccca cgatgccgcc gtgttcaacc
    13141 gcaataccga atattggata cgccgttttc cgacgcagta tctgtttatg tacaaccgct
    13201 ataaaacgcc gtaacgaaaa taaaaatgcc gtctgaacag gtttcagacg gcatcttgtc
    13261 atttgacgat ttcggacaac gaccaacgcg gacggacgtt gaacgcaccg acctccctgc
    13321 ccttgcccag gtgcatcgca ccggcaaagg caatcatggc accgttgtcc gtgcagtatg
    13381 ccatcggcgg gaaaaacacg ctgacttttt cggacggatg tttcggcttg cctttggggg
    13441 tcgggatttg taccgtcatg ttgccgaaag tttcacggag tttgcggttt gcaccgaccc
    13501 cgccggcgac cactacggtt ctgaaccctg tctgcaacag ggcttttttc acttttgcct
    13561 ccaacacttc tactaccgca tcttgaaacg cacggcaaat attgttgcgg gtttgctcgg
    13621 gtatggtttc cgaaccgttt gcctcacgca ctttttcaac ggcagtcaaa acagcggttt
    13681 tcaatcccga aaaactcatc tgcaaatcgt cggaatgaat catcgggcgc ggaaaaacaa
    13741 acgcttcggg cctgcccgat tccgcaagtt ccgacagttt cgcaccgccc ggatacggca
    13801 aacccagcag tttcgccgtt ttgtcgaatg cctcgcccgc cgcatcatcg acgctctcgc
    13861 ccaaaagctc gtaatcgcct atgcccctga ccgccataat ctgcgtatgc ccgcccgaaa
    13921 ccaacagcgc gacaaaagga aagtcgggtt tttcctccgc caacagcggc gacagcagat
    13981 gtccttccaa atgatggacg gggatgacgg gcttatccaa cgctaaagcc agcgcgttgg
    14041 cgtagctcga acccgccagc agcgcaccgc ccaaaccggg cccctgcgta aaggcaaccg
    14101 cgtcaatatc gccatacgat gcgcctgcct gcgccaaaca gccttcagtc aacggaacaa
    14161 ggcggcggat atggtcgcgg ctcgccaatt ccggcacgac cccgccgtat tccgcgtgca
    14221 ttgccatttg agtgtgcagg cagtgcgacc gcaatcctcg ttcggtgtcg tacagcgcaa
    14281 cgccggtttc gtcgcaagaa gattcgattc ctaataccaa catggtctga tgccgttaaa
    14341 aactgaaaaa cgtattctag cgaatttcgg cacgactgcc gtatccccaa aacggaacat
    14401 gccgtctgaa gcccttcaga cggcatcgtc gcaccgtatc aaagcgttcc gtaagaatgc
    14461 agcccgctca aaaacatatt cacgccgata aaggcgaatg cggttacgaa caaaccgata
    14521 atcgcccacc acgccagcac tttgccgcgc cagcccgcca ccagccgcaa atgcagccac
    14581 acggcgtaat tcaaccaaac gatgaacgcc caagtctctt tcggatccca gctccaatag
    14641 cgtccccaag cgtccgccgc ccacaacgcg cccaaaatgg tggcaatggt aaagaacaaa
    14701 aagccgacgg caatcgcctt atacatcacc tcgtcgatca atgccgacgg cggcagccac
    14761 agttttccgc ctttttcttc cgcacgcagg gaaaccagtt cggcaatacc gagcatcgcg
    14821 gaaatgcaga acgcgccgta accgataaag tttgccggaa cgtggatttt catccaccag
    14881 gactggagcg cgggaatcag cggctggatg gtatgcgcct cacgcgacac gctgtaccac
    14941 aacacaaagc cgaccacgac cgccataaag ccgaacacga agccgcccaa tttctgcacg
    15001 gcaaatttac cctcgtaata caaatacatc agcgcggtaa tgaccaaaaa cagaatgaac
    15061 acttcataaa ggttggacac ggggatatgc cctgcatcgg gacggagcag atagctttcg
    15121 tgccaacgta ccagcagacc ggtaaagcct gctacggcag acacccatgc aaacacggtt
    15181 cccataccca acagcgtgtt ggtcggcaca tttttcacgc ttgccaaaac cgcgcccgaa
    15241 atataggcga acagggcgaa gaagacaaaa gcacactgcc acatgatcgc cgattggctg
    15301 ctgaggaaat accgcaacag gaaaatctct gccgatttaa tgttgccgcc atacagaccg
    15361 acggcggcat aggcaagcaa tacgcttaaa ggaacaaacc agcgcatcgg tttgaaaaac
    15421 caacccaaaa acacggcaat accggcactc gcccacaaca tgaccgtttc gtaaatgtcc
    15481 atatgcatac cggaacgggt ctgcacgaaa accgtagccg caaaaaccag cacggcaaat
    15541 acccaatccc aaagattcag attgcggatc aaagatttct gaatcagcag ctcgtgttcc
    15601 ggaagggttt tatagtgttc agtcatgatt caagtccttg ccgagccgtt gcaggctctc
    15661 gacgtgtttt ggaaattcct tctgcaaatc ccgttcgctg cgggccgaag acatagcaaa
    15721 acggattttg ccgtctgaaa acaataccca cgcccgtttt tcgcgcacat aaaacataaa
    15781 taccgtaccc aaaaccaaca ataccgagcc gagatagacc aaaagcgcac ccggcgaacg
    15841 ggtcatctgc aaacctgagg aacgcacctc ggaaaacccg tcaagctgga gcagcatagg
    15901 cgcggaatat tccgtcagcc ccgtataggc atccatactg tgcagcagga aacggttccg
    15961 cgcttcatcc tgccgccatt cgggcaagcc gtaccggcgt atggtttcat ccaaagcagc
    16021 gttcatcacg ccgtaaagca tttcgtagaa atagccctgc atcttatcct gctgcccttt
    16081 cgggatattg gacgtaataa attcgtccaa tcccaaatag cctttttgcg caaagatatt
    16141 cagcgtgttt tccgcagcca gcatgaattg ttcgcggatt tcggcaggtg cgtctttggt
    16201 tgcgtcggca accagacgtt tgcgcccttc cccatctttc aaaaactcac gcaatgccat
    16261 aaaggtgtcc gctttcaact gcttgtccaa ggggatacgc agccagcggt attgctgctg
    16321 caagccgctg cgcgtgccgg tcagccaaaa ataatctttg tcctgcaaaa tcggcagcat
    16381 atagttttta tattcgaccg cctgccctgc cgcatcacgg atgcggtaca cgatggaagg
    16441 gccgatattg gtgtattttt taccttcctg agtaacggcg cggacatcgt tcagagtgga
    16501 tttcaggctt ttttcccgtt ccgcaccctc gctcatgtcc tccacattca tagaagtgaa
    16561 ctgatcgaac tcaagacgat atttgtgttt gccgatttcc aacggaaact ggtgtatgga
    16621 ggttgccttc aacacgacag gttcgcgcga agcatccctc aaattccacg ccttgaatgt
    16681 caaatccgaa ccgccgtcgg caaaactcgc ctgataaatc gtgatgccgt gcaaggtcaa
    16741 aggatggttc acgcggatgg tgcgctcgag tttctcaccg gttgccttgt ccgttacttc
    16801 aatatcgctg gcaaaatcgc gcggcatacc cgtattgtaa aaatcgatat ggaatttttt
    16861 cagtttgact tcaaaaggca agtcctgaac caacatcccg ttgtcggcat tcaggaaaac
    16921 cacatccgca ctttgcccct cggaaatatt gacgttgccc ctaaatgaga gattggacgc
    16981 acccaaaata ctttcgggct tgaaatcctt ggcataaacc gcctgattgt ccggaacaat
    17041 ccgaccggcc agcataccca gcttcagcag caggttactg tctatcaacc cgcccaggca
    17101 aatgacaatc aaagcaacat gggcaaagat atagccccat ttgttcatcg tgcctttttt
    17161 ggcggcaatc agaaccgacc cgtcctcacg gctgacggtt tttccctgaa aaccccgcac
    17221 ctccagataa cgtttggcaa cttcgggggc aatttttaca tccaacagcg aagaatggcg
    17281 catcgccgcc agagattttt ctttaacctt ttcccggaaa gacttcattt cgcgccaaaa
    17341 cggcggaacg ttacggatta aacacaaact ggtagaaacc accagaaaca tcatgataac
    17401 gacaaaccat gccgaagcat agacatcgta caaacccaaa aaatcaaaaa tccgagtcca
    17461 aaacggtccg aatttgacca aataatccgt ctgcggctgg ttttgctgta acaccgtgcc
    17521 gataaccgat gcaataccca gcagactgag caaagcgacc gcaaagcgca tggagctgaa
    17581 aaaagcgaac cacggacggg aaagaagtgt gggggatata cgggatttac tcattgtgtg
    17641 tttattccgc catcaggaat atgggaaagc agaattgggc agacagaaaa caacgtcccg
    17701 attctactgt cttgatgctt tttatttcaa gacaatgaag acagcctgca tcgattccaa
    17761 cggttgcgat tgaaaaaact tatcgcaaaa ttttcagaag ccgtctgaaa accttttcag
    17821 acggcttctg aagcagaccc ttggatgatt aacgcaaacc ctggataaag ttagcaactg
    17881 cttttaaatc ttcttcggac atacggtttg caatatcttc catgatggta tttttacgct
    17941 gaccggactt gtaggcattc atctgttcaa caatatatgc ctggtgctgg ccgcccaaac
    18001 gcggataagc ctgaatttcg cttccgcctc ccggcatacc cgcaccgctc ggaccgtggc
    18061 aggacataca cgccggcact tttttatcgc tcaaaccgcc gcgatagatt ttcgcgccca
    18121 attcgggatt ttccttagga ttggcttcgc cggatttggg ctgctgtttg gcatagaatg
    18181 cggatacgtt caaaatatcc tgatcgctca aattcattac caccggtttc atcacagctg
    18241 ccgaaccgtg ggtgcgtttg ccgtcgcgga tgccgatagt ttgatgatag atgtaagcag
    18301 tatgctgtgc cgccaaacgc ggatacatcg caatgccgct gttaccgtct gctgcatggc
    18361 aagccgcaca aaccgttgcg gcaacctgtt tgcctttttc cacgtctgct ttgggagatg
    18421 cggaaaccgc accggcagcc aaaacaaagg ccaataaagt caatcgtctc atggagtgct
    18481 cctgattaca gcattggata acgcaacaat gctctttttt atattcaaat atgggatttt
    18541 tgacccgatt aaaaccgatg attctgtaaa cgtgttattc tatactaaat ttacattaaa
    18601 ttaccactgt gtttcacata aaaccaaccg catatttttg ctgtcggaca aacggcggcg
    18661 gaaaacaagg atatgcccat gaaccttttt caaaacgcca aattcttcac gacggtaaac
    18721 catctcaaag acctgccgga cacaccgctc gaaattgcct ttgtcggcag gagcaatgcc
    18781 ggaaaatcca gtgccatcaa taccctgacc aaccatgtcc gtcttgccta cgtttcaaaa
    18841 acaccgggac ggacacagca tatcaacttc ttcgagctgc agaacggcaa ttttatggtc
    18901 gatttgcccg gctacggtta tgcccaagtc cccgaagcgg tacgcgcaca ttgggtcaat
    18961 ctgctcggcg actacctccg ccatcggaaa cagcttatcg ggctggtttt gattatggat
    19021 gcccgccatc ctttaaaaga actcgacatc cgtatgctgg actttttcca cacgaccggc
    19081 agaccggttc acatcctgct gtcaaaagcc gacaaattat ccaaaaacga acagataaaa
    19141 accctgtccc aagtcaaaaa actgctcaaa ccttattccg acaggcaaaa catcagcgta
    19201 cagctgtttt ccagcctgaa aaaacaaggt attgacgagg ccaaccgaac tgtcggaagc
    19261 tggtttgacg cagcagatgc cgccgcttcc tctccagagg aaaactgacc tcaattatac
    19321 ggaaaccgta ttccccccac ttgaccggcc gcaaacattt aaaaaattgc cactgccaaa
    19381 tctaaaatgc cgtctgaaag acttttcaga cggcattttg cggagtcttt aaaacaggga
    19441 atccaactgc tgctgtttgg aatccgtatt gctcggaagc accggcgttt cctgtacgtc
    19501 ctgacgcgtt tcgtcagacc ttccctgctg ttcgttttct actgccgctt catcatcttc
    19561 ttttgcccgt cgggaaggtt gcggcgcaat accgctgttg tccagcatca agcccggatc
    19621 ggttaccata cgttccttca tatagtattc gccattgctg ctgaccacac cttcaggcat
    19681 tttcatccct ttgccctgct ttcctttcaa cgcaaaacgc atatagtcca cccaaaccgg
    19741 caccgcaatc gtaccgccgt agccggcacg ccccatactc ttaggtttgt cgaagccgat
    19801 atatacggca gtaaccacat cagggttaaa accgacaaac cacgcatctt tattgtcgtt
    19861 ggtcgtaccc gttttaccgg caatatccgt tcttcccaac gcagctgccc cccttgccgt
    19921 accgacacgg accacatcct gcataatctt atacataata taggcattgc gcggatcgat
    19981 tgcctgaggc gcattttgcc ctgccaccaa aggttgcatt tgggcgcgca acctgccgtc
    20041 tctgtcataa atcttatcga tcacgtgcga agaaacccta tatccgccgt tcgcaaatac
    20101 actatatgcc tccgccactt tcaacggcgt cgtctcgccc gtacctaaag ccatagacag
    20161 gcttgccggc agctcggacg gcctgaagcc gaaacgccgg atatactgtt gcgcgtaacc
    20221 gacaccgata gacatcaaaa tacggatgga aaccatattc ttggaagccg tcagagcctg
    20281 tctcaaagta atgtagccgg aatatctgcc gtctgaattt ttaggtgtcc aaaccgaacc
    20341 gttcggccct ttccccggca gggaaatcgg cgcatcgtta accactgtgg acgcggtcat
    20401 ccccttagat aatgccgccg aatagacaaa cggcttaaag gtcgaacccg gctgccgcat
    20461 tgcctgaacg gcacgattga atgttttgct gtgaaaatca taaccgccga ccagcgcgcg
    20521 cacagctccg gtttttgcat ccagcgaaac caaagccccc tgcggcaacg gctcttgaac
    20581 caccgcccaa cgcccgccgt tgtttttgac acggatgacc gcgcccctgc ggatacggtc
    20641 ctcccccatt ttctcattat cgaccgctcg ggccgcaaaa cccaaggcgc gcctgtcaag
    20701 cgcaacccgc ctgccgccgg gcagctgtat gacgacattt ttctttttag taacatccaa
    20761 cacaacggcg ggaaccattt tatcgacggt atagagtccc gacagatact ggctgacagt
    20821 ctcctcgaca tcttcactct tactcaaatc gatatagttt tccgcaccgc ggtagctgct
    20881 gccgcgatcg aaattccgta gagccttgcg caatgcctcg gttgccgcct tctgatgatc
    20941 ggtgcggacc gtggtataaa ccttaaaacc ctgcgtatag gcatcttcac catatttctc
    21001 atacagttcc cgacgcacca tttccgccac atataaagca ctctgatcga ttttccgaac
    21061 aaaccgctca taatgcagtt cctcattcaa tgcctgatcg cgctgttgca cggtaatcat
    21121 cttctcctcg agcatattgt tcaaaatata cttctggcgc aacttggcac gctccggatt
    21181 aacaatcgga ttataggcag acggagcctt gggcagtccc gcaagcatgg cggcttccgc
    21241 caaagtcaaa tctcggacat tcttattgaa atagatttgc gcggcagatg caaaaccata
    21301 ggcgcgctga ccgaggtaaa tctgattgaa atacaactca aggattttgt ctttgcttaa
    21361 agactgctcg attttatagg caagcaacac ctcattgaat ttgcgtgtga acgttttttc
    21421 actgctcaaa taaaaatttt tcgccacctg ctgtgtaatc gtactcgcac ccgactgcac
    21481 gctgccggac acgacattgc cgacggcagc gcgggcaaca ccccaaacat ccacccccca
    21541 atgccggtaa aagcgtttat cctcggcggc aataaccgca ttccgcaaca cctcggggaa
    21601 atcgccgatt tttgtaaatt cgcgccgctg ctccccatac ataccgatga cttctccatc
    21661 cgccgaataa atagtcaacg gcattttagg ctggtaatgc tgcaaagaat ccaaagacgg
    21721 cagtttcgga tacgttacca aaatagcaat ggcaaccaga cccactccaa atacacaaaa
    21781 accaaaaaac aaaccaaaac aagtagttaa aatcttttta atcatagctg aataataatt
    21841 taccattatt ggtattaaat aaagtaaaat agcaaccgat ttctacaaag cacggtttca
    21901 atgtgcaaag aacaaggaat ccattacgga taccgaaacg gttactcact gtacaaataa
    21961 agcaggaact ttcatcatgc gcttgtttaa aagcttgaaa aaccctaaaa aaacagatgc
    22021 caagctccct aaaaaatctt cgggactcaa taaccgcgcg gcaatcggca tcgatatcga
    22081 ccagcattcc atcaaaatgg tacaattgtc aggacgtagt ttaaaccaaa ttcaattgga
    22141 aaaatacgtc attgccaaat taccaaagaa tatcattcaa ggcaataaag tccaaaatta
    22201 cgatcaactt gttacatatt tgcaacaagc ctatgccaaa ctgggtactt cgtgcaaaaa
    22261 catcgtcgcg tccgtcccgc aaaatctggc aaccatcgaa caattgacct acacggccaa
    22321 agatgcagaa ttggacctgc aggggttcgt ggagtcctcc atctccgaag ccagctcgat
    22381 atcgctcgaa gaagccaatt acgactatca ggtcttgtcc caatcggccg tcggcgaagc
    22441 tgttttgtcc gtcgcatcga gaaaggatga aatcgaaccc ctgattgacg cattcaacgc
    22501 agccggtatg aaattatccg cgcttgatgt ggacattttc ggacaataca acgcctacgc
    22561 gctgtggata aaccattttg cccccgagct tgcggacgaa aaagtggcca ttttcggcgt
    22621 atatgccgca cagacctacg ccctggtcat ccaagacgga aaaatcctat acaaacagga
    22681 aacctccgtc agcgaagaac agctcaacca actcatccag cgcacctatc aggtaacagc
    22741 agaaaaagcg gaagagatca tcaactcccc gcaaaaacct tccgattacc aagaaagcgt
    22801 ggcaaactat ttcaaccagc agattaccca agaaatacaa agggtcttgc agttttatta
    22861 caccacgcag accgcagacg atatgaccga catcaagcat atcctgctga cgggggaagc
    22921 ggtgcgccag aaaggcatcg cccaaaccgt cgcctcgcaa accaatgcgg atgtacaatg
    22981 cgtccatccc gcgcgttatt ttgcgaacga cctcaaaaca gacgaacaac aattcgaact
    23041 tgatgcgccg acactgacca aggcgttcgg tttggcggta cggggattat aattatgaac
    23101 aatttaatca aaatcaacct cctcccctac agggaagaga tgaacaaacg caaacagcag
    23161 cagtttaaaa cgctgatgta cggcgccgtg ctgacgggcg ttgccgccgt tgcggcaacc
    23221 tacctgttta tcgacaatat gatcaataaa cagtcggaaa aaaacacgct gctggaaatc
    23281 tccatcgcac acttggatac cgagctgtcg gaaatacaaa agctcaaaca ggaaaaagat
    23341 gccttcctga ttaagaaaaa caaaatcggg gagctccaga tcaaacgcct ccaagccgca
    23401 aaaatcctcg acagcctgaa tgaggccgtc cccggaagca cctacctaac ctcgctggat
    23461 gccgttaccg ccgactctta tcggctcagc ggcaggacat ccagcgacaa ccgcgttgcc
    23521 gccatgatga gggcgatgcc caataccggc atattcaagc aacccgaatt gttaagcatc
    23581 aagaaaaaca attcgcatca agaatttacc cttcaggcaa cattacaacc tatcgtaaag
    23641 gcggccgaat ccaaagagaa tccggcttcg ggaaacgcac aggaggcaaa ctgaatggct
    23701 tctaaatcat ctaaaaccaa cttggatctc aacaaccttc acctgctcaa ccttcctgcc
    23761 aggcttttta tcgccctgct ggtcgttgcc gccgtgctgg ggctcggtta tgccggattg
    23821 ttcaaaagcc agatggaatc ccttgaggaa tatgaagcaa aagaaaccga actgaaaaac
    23881 acctacaaac agaaaagtat cgacgcggcc agcctgaaca accttaggga cgaacttgcc
    23941 tcaatccgct ctgccttcga tatcatgttg aaacagctgc cgacagatgc agaaattccc
    24001 aatttggttc aagagcttca tcaggcgggt tcgagcaacg gtctgcgctt ggacagcgtt
    24061 atgccccaac ctcccgtaga tgacggtccc atcaaaaaat taccctattc catttccatt
    24121 accggaaatt acgaacagat cagccaattt acccgcgatg tcggcagtct ctcccgaatc
    24181 attacccttg agtcgctgaa aatcgcccaa tctccggaaa acggcggcaa tcctgacggc
    24241 aagagcagta tcctgaacct cagcgccatt gccaccacct accaagcaaa atccgtagaa
    24301 gagcttgccg cagaagcggc acaaaatgcc gagcaaaaat aacttacgtt agggaaacca
    24361 tgaaacacta tgccttactc atcagctttc tggctctctc cgcgtgttcc caaagttctg
    24421 aagacctaaa cgaatggatg gcacaaacgc gacgcgaagc caaagcagaa atcatacctt
    24481 tccaagcacc taccctgccg gttgcgccgg tatacagccc gccgcagctt acagggccga
    24541 acgcattcga cttccgccgc atggaaaccg ccaaaaaagg ggaaaatgcc cccgacacca
    24601 agcgtattaa agaaacgctg gaaaaattca gtttggaaaa tatgcgttat gtcggcattt
    24661 tgaagtccgg acagaaagtc tccggcttca tcgaggctga aggttatgtc tacactgtcg
    24721 gtgtcggcaa ctatttggga caaaactacg gtagaatcga aagcattacc gacgacagca
    24781 tcatcctgaa cgagctgata gaagacagca cgggcaactg ggtttcccgt aaagcagaac
    24841 tgctgttgaa ttcttccgac aaaaacaccg aacaagcggc agcacctgcc gcagaacaaa
    24901 attaagaaga ggattactcc attatgaata ccaaactgac aaaaatcatt tccggtctct
    24961 ttgtcgcaac cgccgccttt cagacggcat cggcaggaaa cattacagac atcaaagttt
    25021 cctccctgcc caacaaacag aaaatcgtca aagtcagctt tgacaaagag attgtcaacc
    25081 cgaccggctt cgtaacctcc tcaccggccc gcatcgcctt ggactttgaa caaaccggca
    25141 tttccatgga tcaacaggta ctcgaatatg ccgatcctct gttgagcaaa atcagtgccg
    25201 cacaaaacag cagccgtgcg cgtctggttc tgaatttgaa caaaccgggc caatacaata
    25261 ccgaagtacg cgggaacaaa gtttggatat tcattaacga atcggacgat accgtgtccg
    25321 cccccgcccg cccagccgta aaagccgcgc ctgccgcacc ggcaaaacaa caggctgccg
    25381 caccgtttac cgagtccgta gtatccgtat ccgcaccgtt cagcccggca aaacaacagg
    25441 cagcggcatc ggcaaaacaa caggcggcga caccggcaaa acaaaccaat atcgatttcc
    25501 gcaaagacgg caaaaatgcc ggcattatcg aattggcggc attgggcttt gccgggcagc
    25561 ccgacatcag ccaacagcac gaccacatca tcgttacgct gaaaaaccat accctgccga
    25621 ccgcgctcca acgcagtttg gatgtggcag acttcaaaac accggttcaa aaggttacgc
    25681 tgaaacgcct caataacgac acccagctga ttatcacaac aaccggcaac tgggaactcg
    25741 tcaacaaatc cgccgcgccc ggatacttta ccttccaagt cctgccgaaa aaacaaaacc
    25801 tcgagtcagg cggcgtgaac aatgcgccca aaaccttcac aggccggaaa atctcccttg
    25861 acttccaaga tgtcgaaatc cgcaccatcc tgcagatttt ggcaaaagaa tccgggatga
    25921 acattgttgc cagcgactcc gtcaacggca aaatgaccct ctccctcaaa gacgtacctt
    25981 gggatcaggc tttggatttg gttatgcagg cgcgcaacct cgatatgcgc cagcaaggga
    26041 acatcgtcaa catcgcgccc cgcgacgagc tgcttgc
//
LOCUS       DAXDSD010000016        26001 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXDSD010000016 DAXDSD010000000
VERSION     DAXDSD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26001
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.016"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(202..849)
                     /locus_tag="ACFB2Q_000531"
     CDS             complement(202..849)
                     /locus_tag="ACFB2Q_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC0917627.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK"
     gene            complement(846..980)
                     /locus_tag="ACFB2Q_000532"
     CDS             complement(846..980)
                     /locus_tag="ACFB2Q_000532"
                     /inference="COORDINATES: protein motif:HMM:NF027394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC0917628.1"
                     /translation="MGLLLSRSGDKEGAFRQFEEEKRLFPESGVFMDFLMKTGKGGKR
                     "
     gene            complement(925..1215)
                     /locus_tag="ACFB2Q_000533"
     CDS             complement(925..1215)
                     /locus_tag="ACFB2Q_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC0917629.1"
                     /translation="MMNPKTLGRLSLCAAVLALTACGGGHKNLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFAEAANKKNECRPGCARPFGTAAFPFGRQRGRVPPI"
     gene            complement(1222..1893)
                     /locus_tag="ACFB2Q_000534"
     CDS             complement(1222..1893)
                     /locus_tag="ACFB2Q_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC0917630.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYIVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            complement(2059..2778)
                     /locus_tag="ACFB2Q_000535"
     CDS             complement(2059..2778)
                     /locus_tag="ACFB2Q_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC0917631.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKEFERFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEARSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(2860..4173)
                     /gene="hpnE"
                     /locus_tag="ACFB2Q_000536"
     CDS             complement(2860..4173)
                     /gene="hpnE"
                     /locus_tag="ACFB2Q_000536"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC0917632.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLRRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            complement(4248..5120)
                     /gene="hpnD"
                     /locus_tag="ACFB2Q_000537"
     CDS             complement(4248..5120)
                     /gene="hpnD"
                     /locus_tag="ACFB2Q_000537"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC0917633.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYVFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            5291..7153
                     /gene="hscA"
                     /locus_tag="ACFB2Q_000538"
     CDS             5291..7153
                     /gene="hscA"
                     /locus_tag="ACFB2Q_000538"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC0917634.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRFIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATFSDGMAIDTSISRAEFHNLTQHLVMKTFEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            7339..7987
                     /locus_tag="ACFB2Q_000539"
                     /pseudo
     CDS             7339..7987
                     /locus_tag="ACFB2Q_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213572.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            8023..8784
                     /locus_tag="ACFB2Q_000540"
     CDS             8023..8784
                     /locus_tag="ACFB2Q_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC0917635.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCV
                     PVGDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDTAWGIDFGDPGQVLPVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            8840..9181
                     /gene="fdx"
                     /locus_tag="ACFB2Q_000541"
     CDS             8840..9181
                     /gene="fdx"
                     /locus_tag="ACFB2Q_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC0917636.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(9241..9781)
                     /locus_tag="ACFB2Q_000542"
                     /pseudo
     CDS             complement(9241..9781)
                     /locus_tag="ACFB2Q_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            9994..10191
                     /gene="iscX"
                     /locus_tag="ACFB2Q_000543"
     CDS             9994..10191
                     /gene="iscX"
                     /locus_tag="ACFB2Q_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC0917637.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(10259..10660)
                     /locus_tag="ACFB2Q_000544"
     CDS             complement(10259..10660)
                     /locus_tag="ACFB2Q_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC0917638.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            10872..11831
                     /locus_tag="ACFB2Q_000545"
     CDS             10872..11831
                     /locus_tag="ACFB2Q_000545"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC0917639.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            11899..13209
                     /gene="tilS"
                     /locus_tag="ACFB2Q_000546"
     CDS             11899..13209
                     /gene="tilS"
                     /locus_tag="ACFB2Q_000546"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC0917640.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            13283..14068
                     /locus_tag="ACFB2Q_000547"
     CDS             13283..14068
                     /locus_tag="ACFB2Q_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC0917641.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADDILTLIDI
                     PLGGTLPDKGDCAVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYLQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            14078..14275
                     /locus_tag="ACFB2Q_000548"
     CDS             14078..14275
                     /locus_tag="ACFB2Q_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917642.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILSSNAVAAAVLFWIWWKWF"
     gene            14537..14889
                     /locus_tag="ACFB2Q_000549"
                     /pseudo
     CDS             14537..14889
                     /locus_tag="ACFB2Q_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14901..15206
                     /locus_tag="ACFB2Q_000550"
     CDS             14901..15206
                     /locus_tag="ACFB2Q_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC0917643.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            15268..16644
                     /gene="mpl"
                     /locus_tag="ACFB2Q_000551"
     CDS             15268..16644
                     /gene="mpl"
                     /locus_tag="ACFB2Q_000551"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC0917644.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            16851..17903
                     /gene="bioB"
                     /locus_tag="ACFB2Q_000552"
     CDS             16851..17903
                     /gene="bioB"
                     /locus_tag="ACFB2Q_000552"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC0917645.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(17999..18772)
                     /locus_tag="ACFB2Q_000553"
     CDS             complement(17999..18772)
                     /locus_tag="ACFB2Q_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC0917646.1"
                     /translation="MQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHSNGFGAQGIT
                     ATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKFKKLIRK
                     TKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGADVVFGQG
                     AHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNIIPITQVGDK
                     IPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGDITRR"
     gene            complement(18810..19112)
                     /locus_tag="ACFB2Q_000554"
     CDS             complement(18810..19112)
                     /locus_tag="ACFB2Q_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917647.1"
                     /translation="MIINKFIWAMAVFSAILALVIGSGFTALEYVKEPPAAPYAVSAP
                     KTAGVKPRRILPERNPPCCRLTVQTPRPFQTPAMRTDRIRLTERQQIPFPSSGSAA"
     gene            complement(19154..21013)
                     /gene="ilvD"
                     /locus_tag="ACFB2Q_000555"
     CDS             complement(19154..21013)
                     /gene="ilvD"
                     /locus_tag="ACFB2Q_000555"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC0917648.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEDILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            complement(21186..22951)
                     /gene="cysI"
                     /locus_tag="ACFB2Q_000556"
                     /pseudo
     CDS             complement(21186..22951)
                     /gene="cysI"
                     /locus_tag="ACFB2Q_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            complement(22978..24771)
                     /locus_tag="ACFB2Q_000557"
                     /pseudo
     CDS             complement(22978..24771)
                     /locus_tag="ACFB2Q_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
     gene            complement(24768..25850)
                     /locus_tag="ACFB2Q_000558"
     CDS             complement(24768..25850)
                     /locus_tag="ACFB2Q_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC0917649.1"
                     /translation="MNYFPIFANLSGRPVLVVGGGAVAARKISLLPKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIA
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNFPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
BASE COUNT         5650 a         6818 c         7495 g         6038 t
ORIGIN      
        1 aatttaatcc actataattt accatacaac cacgccggaa ttaagtttaa atttgaataa
       61 aaggttcggg ttctgcaaaa tacagaaccc gaaccttgtt cggatattga aaccggtcgc
      121 ctgattttgg gcggtgcggc ttgcaagtat caagattcgc atatgccgtc tgaagctcgg
      181 agaggttcag acggcatatg cttatttggg ctgctcttcg acgaatctcg gacctttcaa
      241 gataccgttg cgggaatagg gcgacagtag gttgtatgcg gcggttttgg aaacctgata
      301 accgcggtcg gtcaggctgt tggcaatctg attgaccact gcgccgacca aagcccccaa
      361 caggccgctg ttgctgttgt tgctgccttc gcggatgctg gccgaacccg accacaactc
      421 tttcccgttg cgggaatcga ccagccgtgc tttggcggat acggtcgtca cgctgtctaa
      481 aatttgatat gaagtgccgt attcggtaac cgtaatgtac aaaaccgcat cattgccgaa
      541 aatttgatgc agtttttccg gccggacggc gtgaatatcg gcggcattgg tcaagccgtt
      601 ttctttgaag gtttcctcca cgactgcggc ggggaagacg taatagccgg cttcggaaat
      661 cggcgcggcg gtcgaagcca gcatccccca agtgccgttg acatcaggcg actcgttcag
      721 cggcggaacc accaaaattg aagccggttt gctttctttg aatgacgtgt agtcgaggtc
      781 gggagctttt cgaacttggc aggcagacag agccaacacg gcggcaagcc ctaaaatcaa
      841 aggtttcatc gcttgcctcc tttaccggtt ttcatcagga agtccataaa tacgcccgat
      901 tcgggaaaca gccttttctc ttcttcaaat tggcggaacg cgccctcttt gtctcccgaa
      961 cgggaaagca gcagtcccaa atgggcgtgc gcacccgggg cggcattcat tttttttgtt
     1021 ggcggcttcc gcaaagtatt tttccatctt ttcggtctgc ttgcccaacg aagtgtcgtc
     1081 gtttttcaaa ccttcataga cggtatcggg ataaccgccg taataataca ggtttttatg
     1141 cccgccgccg caggcggtca gagccaagac cgccgcacac agcgacaaac ggcccaaggt
     1201 tttcggattc atcatttctc cttaacggtt ggattgccat gcgccgttgt cgacagcctg
     1261 aaccaagttg tcgacggctt cgcggattgc caagtctaaa actttgccgt tcaaagtcgc
     1321 atcgtagccg gaagtgccgc cgaaaccgat gatttcgcgg ttggaaagtg cgtattcgcc
     1381 cgcgccctgt gtggaataga cgatttcgga agtattgacg ttgacgatat tcagagccac
     1441 ttttgcatag gcgatttgcg atttgccgcg acccaaaatg ccgaagagct gatgatcgcc
     1501 gacatctctg cgtccgaatt cggttacatc gccggtaacg atataatctg cgcctttcag
     1561 gttctgcgct ttgccggaaa tgccggattc ctgtttcaat gcgctaaggt tggtgcggtt
     1621 cagtacgttg aagcggttgg tttgttgcag gtgtgttacc aggatggttt ttgcctggct
     1681 gcccagacgg tcttcactgt cggagaaaat gcctttttgg aagctggagc ggttgtcgaa
     1741 tgttccgacg gaaatcgggg tgcgaacacc gtgatattgc gtattgcagg aggcgacttt
     1801 tgcaacctcg aggctgcgtg aggactcggt cgcacagccg gtcagtgaaa cggcagcggc
     1861 ggcaaaagcg atggcggcgg aaacggtttt cataaaattt accctaaggt ccagttaggg
     1921 aaataacggg ttatcattat tgtccttatg taaatttaag tcaaggtgtt tgtctgtgcg
     1981 ggacggatgc gcgcggaagg tacggatatt tttcaaaacg gaagcgcggc gcgggcggga
     2041 tgcctgcctt ttccggattt agaggtaaac gatttcgccg ctccgccctt tgctttcggc
     2101 acttgcccac cagacaaatg cgggcagtac gtccccgtag cttttgcgtt cgcttctggc
     2161 ttcgcccgga tgggatttga tgcgttgcgg ggaattgatg gggccgggga cgaggacgtt
     2221 ggcgcgcagg ttgccgaagc gttcccattc gtcggcggcg actttgcaca ggtagttcaa
     2281 cgcggctttg gacgcgccga agccgcccca gtaggctttg ggtgtttcgc cgtggctttc
     2341 gccgacgaag atgacggacg cgtcgggcga ctgcttcagc agcgggaaca gggcgcgggt
     2401 cagccccatc ggggcgacgg tgttgatgcg gtattggttg acccattcgg cgacggtttg
     2461 gaaatccagc ggcgagaggg cgtaaaaata gccggcgcag tggacgatgc cgtccagttt
     2521 gccttgcgtg gcttcggcaa tggtggcggc aaaccgttcg aattcttttt cttccgcact
     2581 catcaggtcg aaacggatgg cgaagggttc ggggtgtccg gcttcgacaa tcgcgtcata
     2641 ggctttttcc agttttttct gatggcgtgc caccagaatt acggtcgccc cttcggcggc
     2701 gtaggctttg gcgacctgtt cgcccaagcc ttgcgatgcg ccggtaacta agatggtttt
     2761 gtcggtcagt gtcggcatgg ttgtcctttg ggtttgacgg ttaatgtatt ttagcgtttt
     2821 gccgcacctt gtaaagcgtc ccgatgccgg ccggcgtttt cagacggcat cgttcaggct
     2881 ttgcaggcag gcttccgccg acgcgaaacc tgattgtacg gcggcttcga gcgtggcggg
     2941 gtagtgcggg tggaggtagt cgccggcggg gaagatacgg tgccggtgca gccacgacaa
     3001 atccggcggc ggggcatcgg ttgcggttgt ggcgcgtttt tcggtgatga cgcgcacggc
     3061 ttcgggttcg cccaaatgcg gaaggatgcg tctgaggtcg gcgtgggctt tgtccgccca
     3121 cgcccggttt gcaaacgcgc cgacgcggtc ggaaacgcta atgacggcgg agacttcgtt
     3181 ttcgggcagt ccgagcctgc cccggcaaag cagccattgt gccgtgccgt cggcaatgcc
     3241 ggtcagcggg gcgggcaggc ggacgggttc ggcgtagcgc agatagacgg tggtaatggc
     3301 gtggtagcga aggttttgat atgccgtctg aacgtgttcg ggcgtgcctt cgggcaggag
     3361 cgcggcggcg tggtagggcg cggtggcaag tatggcggca tcgaaggctt cgccgttgac
     3421 gaggactttt ccgtccggga gggtgttcag acggcatacg cgcgtttcga ggcggatgtc
     3481 cgcgccgagc cgttgaagct ccgccaaggc gggttcggcg acgattgcgc ccaaatcctg
     3541 tttgggtagg agatagtcgc tgccggattt tttcgtcagc acgccgtcgg acaaaacgtt
     3601 gcacaacacg cgcaggcttg cggtttccaa aggcgtgttg agcgcgcccc agaccaaggg
     3661 ctgccaaaac tgcattacgg cggcacgcgg cacgttccgc tgtttcagcc attgtgcaac
     3721 tgtcgtgtcg ggctgtccga ggcgtgcgga cttctgcaaa tcggacatat cggcaagcag
     3781 tttggctttg aatgcggacg gtacgcgccg ggcaagcagc acgccgccca aaatatgcag
     3841 cggcgcgggc agggggaggg cgcggaactg caaaccgccg tgcatatgcc agtgcagcgg
     3901 tacgcgcaaa aaggcggcac gggggtctga accgatggtt ttcatcaggc gcaacacgcc
     3961 ccggtatgcg ccgagcaaaa tgtgctgccc gttgtccaaa aaaccgaaac cgtcggtatt
     4021 tccggccagt gtgcgcgccc ttccgcccgc ctgccggccg gcttcaaaca gggtaacgtc
     4081 ggcgtgccgc gccaaggtga cggcggcgga caagccggcc cagcctgcgc cgatgacggc
     4141 gattttcggg cgcggatgcg gcgtgttcat catttattcc tccaatggtt gaaaccccgt
     4201 ttttcggggc ggcgggggtt gggttttcct gaagggcagt atgcgcctta tgcccgttcc
     4261 ggcgtgccgg ggcgcggttt gaatccgaat aaccaggttt tcagggcaat gcgttttttg
     4321 cgcggcgaag ggagggcgat tttgtatttg aggacgtttt gcgcgccgtc tcggtcgatt
     4381 tcgttcaaca gcgcgtaata aaccgccgcc ataaccagtc cgactttttg ggctttttta
     4441 tcggcatcag gcagcagcga tacggcttcg cggtaggttt cacgggcgcg tttgatttgg
     4501 aacgccatca attcggcaaa attgcccgtc gggctgcatt gcaaaatcac gcttgcgggt
     4561 acgtcaaacc gctgcatttc ctccatcggc aggtaaatcc gtcccctgcg cgcatcttcg
     4621 ccgacatcgc ggatgatgtt ggtcagttgc agcgcaagac ccattttatc cgcgtattcc
     4681 agcgttttgc cgtctgaaaa ccccaaaatc cgcgcaatca ggcagccgac cacgcccgcg
     4741 acgcggcggc aatacagttt caattcctca aaactgccgt aacgcgcctg aaccaaatcc
     4801 atctgcatcc cgtcgattaa ggcttccagt tcatatttcg gcagcttgaa ggtttcctta
     4861 acctgccgca aggcctgatt gacggggtgt tccggcatcg cgccgccgaa cgcattgtcc
     4921 aaatcgccgc gccaccagtt caatgttgcc tgtgcaacat cggggttgga acattcgtca
     4981 accacatcgt ccaattcgcg gcaaaagaca tacagaaccg ttaccgcatc ctgtttttcc
     5041 tgagtcagga aacggaagcc cgacaaaaaa ctggagcggc tttcctctgc tttttggcgg
     5101 caatagtcga gtcctttcac gatttatatt cctaatgatg ggcgggaaag gcggatttta
     5161 tcggtatttg gcggtagagg gcaatttcgg cggcacggcc caatccttag cggtttgctc
     5221 aactatcggc gcaaattctg ttaaaatgcc gccttccttc ctttacacac cgcaccgaca
     5281 ggcaaaattt atggctcttt tgcagatttc agaaccgggt atgtccgccg ccccgcaccg
     5341 gcaccgtttg gcggcaggca tcgatttggg tacgaccaac agcttggtcg ccaccgtccg
     5401 cagcggcagt gccgcctgcc tgcccgatgc cgacgggcgc gttaccctgc cttccgtcgt
     5461 ccgctatctg gaaaacggcg gcattgaagt cggcaaaacc gccctgtccg cccaaaaaac
     5521 cgacccgctg aacaccgtca gctccgccaa acgccttatc gggcggactc ttgccgatct
     5581 gcatcaaaat acgcattacc tgccttaccg tttcggcgac aatcaacgct ttatcgaact
     5641 gcatacgcgg cagggggtga aaacgcctgt cgaagtgtcg gcggaaatcc tcaaaaccct
     5701 caaattgcgt gccgaagaaa ccttgggcgg cgatttggtc ggcgtggtga ttaccgtccc
     5761 cgcctatttc gacgacgcgc aacgccaggc caccaaagat gccgcgcgtc tggccggttt
     5821 gaacgtattg cgcctgctca acgaacctac cgccgccgca atcgcctacg ggctggacaa
     5881 cgcctcggaa ggcacgtttg tcgtgtacga tttgggcggc ggcacattcg acgtatccgt
     5941 gttgcaactg accaaaggac tgtttgaagt caaagccacc ggcggcaaca gcgcgttggg
     6001 tggcgatgac ttcgaccatc gtttgttctg ctacctgctc gaacaaaaca gactctccca
     6061 actcaacgaa caagacagcc aactcctgct ctcgctcgtc cgcgccgcca aagaacaatt
     6121 gaccacgcaa accgaagcgc gcattcaggc gacgttttca gacggtatgg caatcgacac
     6181 aagcatcagt cgcgccgagt tccacaacct gacgcagcat ttggtgatga aaacgttcga
     6241 gccggtcaaa caggcgttga aagatgccgg tgtcggtaaa aacgaagtca aaggcgtggt
     6301 tatggtcggc ggttcgaccc gtatgccgca cgtccaacag gcagtcgcca cctttttcgg
     6361 acaaaccccg ctgaacaacc tcaatcccga cgaagtcgtc gcgctcggcg cagccataca
     6421 ggcaaacgtc ctcgcaggca acaaagccga cggcgaatgg ctgctgttgg acgttacccc
     6481 cttgtcgctc ggtttggaaa cctacggcgg cttggcggaa aaaatcatcc cgcgcaattc
     6541 caccatcccc accgcgcgcg cgcaggactt taccaccttc aaagacggtc agaccgcgat
     6601 gacgatacac gtcgtgcaag gcgaacgcga gctcgtttcc gactgccgca gccttgccaa
     6661 atttaccctg cgcggcattc cgcctatggc ggcgggtgcg gcgcgtatcc gcgttacctt
     6721 ccaaatcgat gccgacggtt tgctgtccgt ttccgcccaa gaacaaagca ccggcgtaca
     6781 ggcgcaaatc gaagtcaaac cctcctacgg tttggacgat gacaccatca cccaaatgct
     6841 caaagacagc atgggcaacg ccgccgaaga tatggcggca cgcgcccgcg ccgaagccgt
     6901 ggtcgaagcc gaaagcctga ccgatgccgt caacgccgcc ctcgagttgg acagcgattt
     6961 gctggatgcc gaagagtttg cacaaatcca gcgagacatc gccgatttgc aaggccgtct
     7021 gaaagacggt aaagctgaag acatccgtgc agctgtcgcc aaactcagcc gcagcaccga
     7081 caatttcgcc gccaagcgca tgaaccgcaa catccaacgt gcgctgacag gtcagagtgt
     7141 cgataatatt tgatacttaa acggtttcag acggcataga tataacctga tgccgtctga
     7201 aggatcgaaa acacttgaaa aacatcgata tggaaaagtc aggcattgtc tattcgatga
     7261 aaaccgtcat caagggcgtg tatagtgaat taaatttaaa ccggtacggc gttggctcgc
     7321 cttgccgtac tatttgtact gtctgcggct cgccgccttg tcctgatttt tgttaattca
     7381 ctatataaaa tcggcatttc ggacgtaagc aattttgaag gcagaatgcg ccatttggaa
     7441 aacaacggct atgcgaacgt tgccgggttg gaacgcctcc tcgccgtcaa aaccgacaat
     7501 tacaaagaaa aaaaacctgc tccatgaaat tttcagcaaa agcaggatag gcgataccga
     7561 attgttcgcc gtggacgaaa accttgtgaa acgtttgttt ttatcgcttc gcggcgaaat
     7621 cgtgttcccg aaaaacgaaa cggcggaatc ggaatttgaa aaaagcgtcc acgaacgccg
     7681 gcaggaaggg aatgccgggt ccggccgcaa acaactgctt gatttggtac ggcgcggaca
     7741 ccgggaatac ccttacgcgc tgccccggct tttggcggac gcggcatcct acaagccgaa
     7801 aaaatcgaaa atccgccttt ttaaagaagc atatttcggc aaaagcggca cgaggctgac
     7861 cgacgaaatt gcagacggca tccatattta cacctgtttt tcgcgggcgg atttggaaaa
     7921 agcctattcc gaatatttgg aacttttcaa atccgaatcg gatgccggag gtagaaaacc
     7981 gcggtaaggt gcaaacagat accgtacacg ttgaggagca gtatgatggg cgattccgtc
     8041 atttattatg tagaacaggc agacgaaccg gtaaaccgtg ccggcgaacg cgcccgtaaa
     8101 acattcaaat acttttggcg cgagcttttt tgggaacgcc gccgcattat ttccgccttg
     8161 gattttgcca tggtcaaagt cccttttttc caagacggcg aagacggcga aatttgcgaa
     8221 catatgtgga tcgacgatat ttattttgac ggtctttata tttacggtgt gctgaacaat
     8281 gaacccggcg gactgaccaa tgtcgaacaa ggcgaaagcg tttgcgttcc ggttggcgac
     8341 atcagcgact ggatgttcgt gtgcaacggc atcccctacg gcggctttac cgtacaggca
     8401 atgcgcggac agatgacgga agaggagcgt accgaacacg ataccgcctg gggaatcgat
     8461 ttcggcgatc ccgggcaggt attgccggtg tatgaagaaa aagaacatcc cgaaaatttg
     8521 gaagagcatc cgatgtgccg gaactgtatt gacgattttc ggcaacagtt gtcccaaaat
     8581 ccggattttc tgcatgagca ggacgaagac ggctatacgc cgcttcatca tgaagccatg
     8641 gcaggaaatg cacttatggt tcaagccatg cttgaatacg gcgcaaatcc tgcctcaaag
     8701 acatcggaag gctataccgc cctcgatttt gcccgcctga cgggctggca aaatgttgcc
     8761 gacctgctcg aaccgcgaca ttaggcagac agttttccta aaaacggaca caaatacttt
     8821 ttacagaaag acaataaaaa tgcccaaaat caccgtactt ccacacgcaa cattatgccc
     8881 cgagggtgca gtcatcgata acgcgcccga aggcaaaacc gtcctcgacg tgctgctcga
     8941 ccacgacatc gaagtcgatc acgcctgcga aaaatcctgc gcctgcacca cctgccacgt
     9001 gattatccgc aaaggtttcg acagtttgga agagccggcc gaattggaag aagacctgct
     9061 cgatcaggct tggggtttgg aagccgattc gcgcctgagt tgtcaggcgg ttgttgccgg
     9121 cgaggatttg attgtggaaa tccccaaata caccatcaac cacgcgcgcg aagaacactg
     9181 aaaacaggcc gtctgaagcc ggcacgcttc agacggcatt gttgcgcgga taaggcgcaa
     9241 ttaccgaaaa acagacgttt gtgaagacgg aactttcgat tctatattga tttttatcgg
     9301 ttttttgacg gaacgacccg atacggcatc tgaggcggct tgcgtttctt cctgcccgcc
     9361 tgccggattt gcctgtcctt gcgcgaaacc gaaagagacg gcggcgcggc ggacaaggtc
     9421 gagatagcgt ccttcaagct cggggcaggc ggcggacacg ctttctgccg taaccgtgaa
     9481 gccgcccgac cgactcggaa aggcgttccg caatctgcat gcagatgcgc caacgccggg
     9541 cgcgcaacat tgccgcatcg caaccgacag ccgctttatc caaagcaatc agaccgcgcc
     9601 tgctccggta gttaaggcaa accgttactc tgtagagggt atgttgcccg tccaatattt
     9661 gatacagttt gcgtatcagc agaatcaggc ggtcaaactc ctccatctcc gacagggagt
     9721 cgggatggtc ggaagcggta taaaccagtt cggacaattt tgcggcaaac atattgttca
     9781 tcaatcttcc ttatcggttg aaaccccgct cttcagggcg gtagaatcag atttgtttgg
     9841 gaggggtaac tcctcccgga tcaggacgac acataaggtg gtgcttgatg tgccgtccgg
     9901 cgagttgaaa cattcagcca tcctcaaggg gcggcagttt tgccgaaaca tattctacac
     9961 ggcttcaatg ccggacgata aaaggaaatt tatatgaaat ggaccgatac ccggcgcatc
    10021 gccgaagaac tctacgacct gcacggcgaa gccatcgatc ccaaaaccgt gcgctttacc
    10081 caactgcgcg acctgattat ggcattgccc gaatttgacg acgatcccgc ccgttgcggc
    10141 gaacgcatcc ttgaagccgt gcagcaggca tggatagacg aggcggaata agtttcagga
    10201 atgccgtctg aaatgcggcg gtacgcggtt cgtgcttctg tttgcagcgg gaatggtttt
    10261 accagtctcc ttttttcagc ctgtccagtt ggcggcggtc gcgcttggtc ggtctgccgt
    10321 cgggatgggc ggaagtgatg cggctgaatt ggtcgagctg tttgcactct tccctcaatg
    10381 ctgccgtttt cgcgtcttct tcatacagaa gccgcgcctc gggtgccggg cggcgttggt
    10441 ggttcaaacc tttaaccttg attttatggg gaagggaatt gagcgtcagg tcgataatat
    10501 cgccgatgtc tatggtttta ctgtttttga ctttcgagcc gtttacttga accctaccca
    10561 gttcgatatg cttttgcgca agggaacggg tcttgaaaaa acgtgccgcc caaagccatt
    10621 tgtccagccg catggcggaa gaatcgtgct tgtctttcat acgattttgt ttgaaataat
    10681 tgaatttgtt tcgagtttag cataagatac atcgccttat aactagtata tatgcactaa
    10741 tccgctgttt tccatgctgt ccgaacacaa aaagagggtg tggaaaagcc gttttggaca
    10801 ataaattaac tgcggaatat gcacaaatag cgtatgatag cggcagaatc tgttgatgag
    10861 agcttcattt tatgaaacct gtttttttgg attttgaaca acccatagcc gaactgacca
    10921 acaaaatcga tgagctgcgt ttcgtccaag acgagtctgc cgtcgatatt tcggacgaaa
    10981 tacaccgttt gcagaaaaaa agcaacgatt tgaccaaatc gattttcagc aaactcacac
    11041 ccgctcaaat ttcacaggtt tcccggcatc cgcagcgtcc ctatactttg gattacattg
    11101 acgcgctgtt taccgatttt gaagagctgc acggcgaccg ccactttgcc gacgatcatg
    11161 cgattgtcgg cggattggcg cgtttcaacg gacaaagcgt tgtggtcgtc gggcatcaga
    11221 aagggcgcga caccaaagaa aaaatccgcc gcaacttcgg tatgccccgt cctgaaggct
    11281 accgcaaagc cctgcgcctg atgaaaacgg cggaaaaatt cggcttgccc gtcatgacct
    11341 ttatcgatac gccgggcgcg tatcccggca tcggcgcgga agaacgcggg cagtcggaag
    11401 ccatcggcaa aaacctgtac gaactgaccc gactgcgcgt tcccgttttg tgtaccgtca
    11461 tcggcgaagg cggttcgggc ggtgcgttgg cggtcgccgt aggcgattac gtcaatatgc
    11521 tgcaatattc gacctattcc gttatttccc ccgaaggctg tgcttcgatt ttgtggaaaa
    11581 ccgccgaaaa ggcggctgat gcggcacagg ctttgggtat tactgccgac cgcctgcaaa
    11641 agctggactt ggtcgatacc gtcattaaag agcctttggg tggtgcgcat agggatttcg
    11701 ggcaaaccat gaaaaacgta aaagccgttt tggaaaaaca actgcacgaa gcgcaaagca
    11761 tcccgcttgc cgatttgctt tcgcgccgtt tcgaccgcat tatggcttac ggcaaatttt
    11821 cggaacaata attcgggtag aacaagcagc aagcagtttg tctgaaactg cttgcttttt
    11881 ctttatcggg atgaagccgt gctgacttta gatgcgtttg agcaatgctt gaaggattgt
    11941 tttcctcaag gtctgaatgg aaaaaaaaca gcagtggcat taagcggcgg cttggattcc
    12001 gtcgttttgc tgcatctgct ggtttgtgcc gggaagcggg caggatttgt gccggaggca
    12061 ttgcacatcc atcacggctt gagtccccga gccgacgatt gggcggattt ctgccggaat
    12121 tattgcgata tgctaggggt ggggctggaa acggttaagg tctgcgtgga aaaaaacggt
    12181 ttgggcatcg aggcggcggc aaggcaaaag cgttatgccg agtttgccga aaaaggcttt
    12241 gacgttttgg cgttggcgca ccacagggac gatcaaatcg aaacctttat gctggcagtc
    12301 gcgcgcggcg gaggtttgcg cgctttggcg gctatgcccg ccgtccgccc tttgggggaa
    12361 aacggcatta tctggcggcc tttgctgcct ttttcgcgcc aagacatatg ggattatgcc
    12421 cgaaaacacg gtttgccgaa tatcgaggat gaaagcaata ccgatacggc ttatttgcga
    12481 aaccgcttcc ggcaccgtat tttgcccgaa ctttcggcgc agattcccca tttcgggcgg
    12541 catgtgctga acaatgtccg cgctttgcag gaagatttgg ctttgttgga agaggtcgtc
    12601 gttcaagact gccgttgggt ttgcggggcc ggttatttcg atacggcgcg gtggctgacg
    12661 ttttccccgc gccggaaaac ccatattttg cggaattttc taaaggaaaa cggcatcccc
    12721 gtgccgaatc agaacgcact tgccgacatt gcccgcgttt tgacggaggc aaaaaccgga
    12781 cgttggaact tgcaaggctt tgaattgcat cattatgcag gcaggctgtt tgtgtttgcg
    12841 tcggaacagt tggcaaaacc ggcttttctg aaagatggga cgataagcgg aaatctcaaa
    12901 aaaatactga cagaacatag atttgtgttg aagcggcatc cgttcggact tcctgaagcc
    12961 atgttggaac aggacgggat tttgaggacg gtagcggcat cggatacgtt ggctgtcggc
    13021 ggcatccata aaaatgtgaa aaaaatcctt caggggaaac gggttctgcc tttcctgcgc
    13081 ccaatttggc cgctcgttgc cgacagcgga aaccgtccat tggcgttggc aaactgttgt
    13141 gcggattttc aaatctcggt ttcagacggc attttgcccg tacatcctga ctttcccatt
    13201 ttattttgat aatatcgcaa acagatttcg gcggcgttca gtcgggtatt gtccggttgc
    13261 atatttctaa aaggcttgtg aagtgaaaca catcagttcg accaataatg aacatatcag
    13321 acacctgcac cgcctgttgt cgcaaggaaa gttcagacgg cagtatgccc aaaccgtttt
    13381 ggagggcgtg cacctgcttc aggttttcct gcaatccggc agaaagccgg tcggggtata
    13441 tattcccgaa gctaaaatgc cgtctgaaga agtccttaaa ttgacagcgg ttttgccgga
    13501 agacgggatt ttttccgttt cagacggcat attgaaaaaa atcagcagct tgagttgtgc
    13561 agacgatata cttacactga ttgatatccc attgggcgga accttgccgg acaaaggcga
    13621 ctgtgcggtt ttggacggcg tgcaagatcc gggcaatgtc ggcaccgtct tacggagtgc
    13681 ggcggcggca ggggtaggta cggtcgtttt gggcaggggt tgtgcggacg cgtggtcgcc
    13741 caaggtatta cgcgcgggta tgggcgcgca tttcttgttg gacatttatt tgcaggcgga
    13801 tttggaaata tggttggcgc actatgaaga ccgtgtgttt gccaccgcgt tgcgcgagga
    13861 aaagcaggcg gttttgtacg gcgaagattt gtgcgaaccg acagcttggg tgttcggcaa
    13921 cgaaggtgcg ggtgtcggta aagcagtttt agacagggcg gacaagtgtg tcaggatacc
    13981 gatgcacgat gcaaccgagt ctttaaatgt cgcgatggcg gcgacaatct gcctgtttga
    14041 acagatgcgc caacgggcgg cgtattgagg aagagaaatg ccgtctgaaa aaatctatta
    14101 cggcgtattg attttcttat gtatcgcttc tatgctgctg tcaccgtttt tttatgcggg
    14161 tgctttgaag cccaagaagg cggcattgcg gaaggacggg cagtggaaac tcatcttatc
    14221 gtccaatgcc gtggcggcgg cggttttgtt ttggatatgg tggaaatggt tttgacagat
    14281 attgctcaaa atcgtgctaa tggaatccga acaaataaag aatttggtaa aaaatttgtt
    14341 aaatcaacgg attaaagttt tgtggaaaac aaaacagctc taagcaaata gggcgtttgt
    14401 cggtaaatac ggaagagttg cggcattatc gggcatcttt aacaagtagt gtcggcttgg
    14461 caggcaatcg gtttttatgg gcagcttgca aaatcgcgga tataaaattg cgaatcggtt
    14521 aaagtgtggg gacgctatga aaaattgcga atttttttat gatccgacaa gggcaatcta
    14581 tgatagcggt gcagattact taactaggga aaaatataga ttagtcgtaa tcgcaaatag
    14641 cgcttggggc tattgctgaa tttaccttgt tattatgacg aggttttgga aaagcggaaa
    14701 ataccgttcg gcaaacagga aattgatgac gatatggaca aagtgtccgc ccttaagcgg
    14761 aagtttaaag acatttctga aatcaaagta ggggatggtt gggaataccc gttcaattat
    14821 gagcaaggaa tgaaagaatt agatgaagtt ctattgaaat acattccttt ttttgaagag
    14881 aaacaataaa ggagtttaat atgcgcgtat ctaaaatgat tggaagtata ttgcttgttg
    14941 cagcggttca gaccgtattt tcggcaaatg tttacgagtg ccgccataat ggtaaaacca
    15001 gttacagcca aactccggga aaacattgta ccaacgcggg tttggggcgg gatcgggtgt
    15061 acagttcggt caggcctgcc gtgaaagaca gggcggaaga cgcaggagtt ggcgattatt
    15121 cggacacggt gagggacgaa gccgtccaaa atccgaaagg gaatgcacag aaagacggtt
    15181 cggatgccgg catcagaccg cattgattga aaccgaatca accattacgg tgttgaacaa
    15241 tagaatttga acgattaggg aactctgatg aaacacatcc acattatcgg tatcggcggc
    15301 acgtttatgg gcgggattgc cgccattgcc aaagaagccg ggttcaaagt cagcggttgc
    15361 gacgcgaaga tgtatccgcc gatgagcacc cagctcgaag ccttgggcat aggcgtacac
    15421 gagggcttcg atgccgcgca gttggaggaa tttcaagccg atatttacgt catcggcaat
    15481 gtcgccaggc gcgggatgga tgtggtcgag gcgattttga accgtgggct gccttatatt
    15541 tccggcccgc aatggctggc tgaaaacgtg ctgcaccatc attgggtact cggcgtggca
    15601 gggacgcacg gcaaaacgac caccgcgtcc atgctcgcct gggtcttgga atatgccgga
    15661 ctcgcgccgg gcttcctcat cggcggtgta ccggaaaatt tcggcgtttc cgcccgccta
    15721 ccgcaaacgc cgcgtcaaga cccgaacagc aaatcgccgt ttttcgtcat cgaagccgac
    15781 gaatacgaca ccgccttttt cgacaaacgc tccaaattcg tgcattatcg cccgcgtacc
    15841 gccgtgttga acaatctgga attcgaccac gccgacatct tcgccgactt gggcgcgata
    15901 cagacccagt tccaccacct cgtgcgcacc gtaccatccg aaggcctcat cgtctgcaac
    15961 ggacagcagc aaagcctgca agatactttg gacaaaggct gctggacgcc ggtggaaaaa
    16021 ttcggcaccg gacacggctg gcagattggt gaagtcaatg ccgacggctc gttcgacgta
    16081 ttgcttgacg gcaaaaaagc cggacacgtc gcatgggatt tgatgggcgg acacaaccgc
    16141 atgaacgcgc tcgccgtcat cgctgccgca cgccatgccg gagtcgatgt tcagacggcc
    16201 tgcgaagcct tgggtgcgtt taaaaacgtc aaacgccgca tggaaatcaa aggcacggca
    16261 aacggcatca ccgtttacga cgatttcgcc caccacccga ccgccatcga aaccacgatt
    16321 caaggtttgc gccaacgtgt cggcggcgcg cgcatcctcg ccgtcctcga gccgcgttcc
    16381 aacaccatga aactcggcac gatgaagtcc gccctgcccg caagcctcaa agaagccgac
    16441 caagtgttct gctacgccgg cggcgcggac tgggacgttg ccgaagccct cgcgcctttg
    16501 ggctgcaggc tgcgcgtcgg taaagatttc gataccttcg ttgccgaaat tgtgaaaaac
    16561 gcccgaaccg gcgaccatat tttggtgatg agcaacggcg gtttcggcgg aatacacacc
    16621 aaactgctgg acgctttgag atagcccggg caatgccgtc tgaaagccct ttagacggca
    16681 ttgcccggct gcgcggcaca aaggcggaaa aaccgtttgc cccgtatttt caaacgcgtt
    16741 acacttgccg ccgctgtttt cagccatttg attacccgca accgccgtca ttgcgccggc
    16801 ggtttgcctg tcagcgtcat tgcgccgctg taaatacgaa agaacacatt atgaccgtat
    16861 cccccgtcgc cttgcgccgt aagaccgagt gcaagcctca tcccaccgcg cgctattgga
    16921 aaaaatgcga tgtcgaggca cttttcggac tgccgttttt agaacttgtc tatcaggcgg
    16981 cagaagtcca ccgccaaaat ttcaacccgc gcgaaatcca gctttccacg ctgttgtcca
    17041 tcaaaaccgg cggttgtccc gaagattgcg cctactgtcc gcaatcagcg caccacaaca
    17101 ccaatttggg caaagagcag atgatggatg tggatgaaat cgtcgaaaaa gccaaaatcg
    17161 ccaaatcgcg cggcgcaagc cggttttgta tgggcgcggc atggcgcggc cccaaaccca
    17221 aagacgtgga gacggtttcc gcaatcatca aagccgtcaa gggcttaggt atggaaacct
    17281 gcggcacgtt cggtatgctc gaagaaggta tggcggaaga cttgaaagag gcgggcttgg
    17341 attattacaa ccacaacctc gacaccgacc ccgaccgcta caacgacatc atccacacac
    17401 gccggcacga agaccgtatg gataccttgg gcaaagtccg caacgccggt ttgaaagtct
    17461 gctgcggcgg catcgtcggg atgaacgaaa cccgcgccga acgcgccggg ctgattgcca
    17521 gcctcgccaa cctcgacccg cagcccgaaa gcgtgccgat taaccggttg gtcaaagtgg
    17581 aaggcacgcc gcttgccgat gccgaagatt tggattggac cgaatttgtc cgtacggttt
    17641 ccgtagcgcg gattacgatg ccgcaaagtt atgtccgcct ctcggcaggg cgcagcaata
    17701 tgcctgaagc gatgcaggcg atgtgcttca tggcgggtgc gaactcgatt ttttacggcg
    17761 acaagctgtt gaccacaggc aatcctgatg aggacggcga tagaatcctg atggaaaagc
    17821 ttaacctgta tcccttgcag tttgagccgg aaggcgaggt cgccgaagtg gaaaaagcct
    17881 ccgggattaa agcggattat tgacgattga aaaatgccgt ctgaaacttg aatgccgtct
    17941 gaaacctgaa aaaaggcttt cagacggcat ttgttcggac ggaatttcca atatcttttt
    18001 accggcgcgt gatgtcgccg ccgggcgaga catccagtcc gttcccgttg gggaagtcgc
    18061 tatggttcag ataaataatc cgctcaataa cagttttttc agggtcgatt ttggggattt
    18121 tatcgccgac ttgagtgatg gggataatgt tgccggaaac gaaccgcccc tgtttgtcgg
    18181 tgataatttt gaaaatcggc gcaatgccgc tgatgccgta ggtggcaaag ttgccgccgc
    18241 tgtaagagat gaagcggtcg tggtaaagtt cgacggcgcg agtaacgtgc gccccctgcc
    18301 cgaatacgac atccgcgccg gaatcgacgg cgagccgcgc aaactcgacg acgttgcccc
    18361 tgttttcccc atagaagatt tcggtatcga acggcaggtg ttcagcctgt ttcccttccg
    18421 cgccgccgtg gaacatcaca atgacgatgt cggttttttg tttggttttc cgaatcagtt
    18481 ttttgaattt ggcgtaatcg ttcagtttga cggcggcaag gttgggggcg aaggagacga
    18541 agccgtatct cacgccgttt ttcttcagga tggcggtttc aaacctgttt tcgatgcccg
    18601 aatatttgat gttcaattcg tcaaggttgc ctgccgttgc cgtgatgcct tgcgcgccga
    18661 agccgttgct gtggttgttt gcgaggctga ggtagtcgaa tcccgcgtcg gcaaggtatt
    18721 gcccgtatgc ggagggcgtt cggaatgcat agcatatttt ggtggtttgc acattttttc
    18781 ggcgtaccgc cttcgtcaaa cagcgtacct tatgccgccg accccgatga tggaaacggt
    18841 atctgctgcc tttccgtcag gcgtatcctg tccgtccgca tcgccggtgt ttgaaacggg
    18901 cgcggggttt gaaccgtcaa tcggcagcac gggggattcc gttccggcag gatgcgccga
    18961 ggcttgaccc cggcggtttt cggcgcggaa acagcataag gggcggcagg cggctctttt
    19021 acatactcga gcgcggtaaa gccgctgccg ataacgaggg ccaaaattgc ggaaaatacc
    19081 gccatcgccc agataaattt gttaataatc atacctttac tgttcagacg gcatttgccg
    19141 cacgttttgg ggcttatctc tcgatttgcg atacgtcgcg caccgcgcct ttgtcggcgg
    19201 aagtcgccat cgcgccgtaa gcccttaatg cggcggagac gtagcggtcg cggttttccg
    19261 gtttccacgc tttgctgccg cgagcttcca tttcggcacg gcgtgcggca agctcttcat
    19321 cggaaatgac aaggcggatg ctgcgtttgg ggatgtcgat ttcaatcgta tcgccttcgt
    19381 gtaccaaacc gattgcgccg ccttccgccg cttcgggcga ggcgtgtccg atggacaaac
    19441 ctgatgtgcc gccggagaag cgtccgtcgg ttaagagggc gcaggctttg cccaagcctt
    19501 tggatttcag gtaggaggtc gggtatagca tttcctgcat acccgggccg cctttcgggc
    19561 cttcgtagcg gatgatgacg atgttgccgg cgacgatttg gttgcccaaa atatcttcga
    19621 cggcggcttc ctggctttca aacacgcgcg cgcggccggt gaatttgagg atgctctcgt
    19681 ccacgcctgc ggtttttacc acgcagccgc gctcggcgat gttgccgaat aagaccgcca
    19741 aaccgccgtc ttgcgagtag gcgtgtgcca cgtcgcggat acagcctttt tcgcggtcaa
    19801 ggtcgagggt tttccaaata cggttttgcg agaacgcctg agtggtgcgc acgccgccgg
    19861 gagccgcttt gaaacgttcg atggcgcggg tgttttcggg attggtgacg tcccattttt
    19921 caatcgcgtc tttcagtgtc ggcgcgtgga tcgtgtacac gtcggtgtgc agtttgcccg
    19981 ctttgtcaag ttctttcaga atggcgaaga tgccgccggc gcggtgcacg tcttccatat
    20041 agtagtcgtg gttgttgggc gcggttttgc agatgcaggg cacgacgcgg cttaagcggt
    20101 cgatgtctgc cattttgaaa tccacgcccg cttcgttggc gacggcgagc aagtgcagga
    20161 tggtattggt gctgcctccc atcgcaatat ccatcgtcat ggcgttttca aacgcttttt
    20221 tggtggcaat gctgcgcggc agcacggttt cgtcgtcttg ctcgtaatag cgtttggtga
    20281 tttcgacaat catacgccct gcttcgagga acaattcttt gcggcctgcg tgggtcgcca
    20341 aatacgaacc gttgccgggc agggacagac cgagcgcttc ggtcaggcag ttcatcgagt
    20401 ttgccgtaaa catacccgaa cacgagccgc aggtcgggca ggcgttttgt tcgacttctt
    20461 cgacttgccg gttgctgaca ttgtcgtccg ccgattcgat catggcgtca atcaagtcca
    20521 aacggcgttc gggttggatg tttgccacgc cgatgacctt gcccgcttcc atcgggccgc
    20581 cggagacgaa gatggtgggg atgttcaggc gcatggcggc aatcagcatt cccggggtga
    20641 ttttgtcgca gttggaaatg cacaccagcg cgtcggcgca gtgggcgttg accatatatt
    20701 cgatggagtc ggcaatcaaa tcgcggctgg gcagggagta caacataccg ctgtgcccca
    20761 tggcgatgcc gtcgtcgatg gcgatggtgt tgaattcttt ggcaattgca cctgcttttt
    20821 caatctcgcg ggcaaccagc tggcccatat tgtgcaggtg gacatggccg ggcacgaatt
    20881 gggtgaagga gttggcaacg gcgatgatgg gcttgccgaa gtcggtttcc atcacgccgg
    20941 tggcgcgcca caatgcgcgc gcgcccgcca tattgcggcc gtgggtggag gttttggagc
    21001 ggtattctgg catagtgtgt ttccttgtgc ctataccgtc tgaaaggcag ggctgtttca
    21061 gacggcatcg ggtacggttt tttagagtgg gaaaaggggg tattttatac caagtatcgg
    21121 aattttgcgg aatggaaacg tgtgcggcaa aaaagaacat ccccgcagga atgcggggga
    21181 cgggttcagg cgcgcgcaat cgggacggct ttggatgcgt cccaaaaatc gacgggcgca
    21241 tccaacacgg gtttgacgat gcccgtccgt atggcgaaat cgccgaaacc ttcgtcggta
    21301 tcgcgtcccg ccgcccattt gccgatcagg tcttccaatt cggaaaggat ttccggcagg
    21361 gtgatgtttt ctttgtagag gcgggggatg cgtacgcctt cgcggtcgcc gccgatgtgg
    21421 aggttgtagc gtcccacggc tttgccgacc agtccgattt ccgccaacat cgcccgtccg
    21481 cagccgttcg ggcagccggt aatgcgggta acgatgtagt cgtccgacgc gccgtgttcc
    21541 gccataatct tatccagctc gccgatgaag tccggcaaca cgcgttcggc ttccgccatt
    21601 gccagcgggc aggtcgggaa ggaaacgcac gacatcgcat tttcgcgcag cttgctgaca
    21661 ccgtcgcgta tcagcccgta tgagcgggcg agctgttcga tccgggcttt gcctccttcg
    21721 ggcacatttg ccacgatgag gttttggttg gcggtgatgc ggaagccgcc tttgtggatt
    21781 ttggcgattt ccaacacgcc ggtcagaagc tgtttcccgc cttcgtcaac caaacgcccg
    21841 ctttcgatga aaagggtcaa atgccagttg ccgtctatgc ctttcacccg gccgatgcgg
    21901 tcgccgcgcc cggtaaactt gaacggccgt atgggtttga acggcatacc catacggcgt
    21961 tcgacttccg tgcggaagtt gtccaagccc atattttgaa tggtgtagcg ggtgcggtcg
    22021 tttttgcggt ggctgcggtt gccgaagttg cgctgcgtgg ttaccaccgc ttcggcggcc
    22081 ttcagtgcgt gttccggagg cacgaaaccc agttccagtg aaatgttcgg ataggttttg
    22141 gtgttgccgt tgccgtgttc cgtcgaaagc ccgccgcccg ccaaaacatt gaagccggca
    22201 agctgtccgt tgccgtctga aacggcgacg aaatccaaat cgttaccgta gcagtccaca
    22261 tcgttcaagg gcgggatgac gactgcggtt ttgaattttc gcggcagata ggttttgccc
    22321 aaaatcggct cgccttcttg aaggaagtcg tcggaacttt gaactttttt gccgtccacc
    22381 cacatatcca gataaccgcg cgtgcgcggc agcaggtgtt cggaaatctt tttcgcgtat
    22441 tcgtaagcct gccggtgcag ctcggactcg atcgggttgg aggtgcaaag cacattgcgg
    22501 ttcatatccg ccgccgtagc gatggaatcc aaacccaggc tgtgcaagag gcggggcatc
    22561 gtctgcaact tggttttcgg cacgccgtga aattggaagg tttgccggtt ggttagccgg
    22621 atggagcggt aatgactctt ttcccgggcg aatttgtcca gttctatcca ctgggacggt
    22681 ttgatgatcc cgccggcagc cggcagcgca aaagcataaa tttcaagggc tcgagttttg
    22741 cctcggtgcg gatgtcgcgg ccatcctgct catacatact gtggaagcgg atgagttgga
    22801 agttgtcgcc tttgaagccg cccgtgagcg ggtctttcaa atcgtccaaa atcgtgccgc
    22861 gtaaaaaatt gctttcggtt ttcaggcgtt cgttgtcgga tagcggcttt tcttgccacg
    22921 ccaaaccttt tgtcttggcc tgtacggtca ttttgtgttc ctcccgatta tatttaatca
    22981 ataaacatca cgctgatagc gtttttcttc gcgcagcata tccaaatatc cttctgcgcc
    23041 gtcttcgtcc gaatgccctg ccccgataat cacatccagc aaggcggctt ccacttcttt
    23101 tgccattttt gccgcatcgc cgcacacata gatatgcgcg ccttcctgca gccattgcca
    23161 aagtccttcc gcctgttcgc ggattttgtc ctgcacatag attttttctt cctgatcgcg
    23221 cgaccaggcg aagtcatatc tgtgcaggaa gccgtctttg gcaaactgct gccattcggt
    23281 ctgatagagg aagtcggcgg caaaatgcgg attgccgaaa atcagccagt ttctgccttc
    23341 cgcattttct gcggcacgtt gttggacgaa ggcgcggaac ggtgcgacgc cggtaccgga
    23401 gccgatcatc acaatcggct tgcggctgtc ttcgggcagc ctgaagccgt cgttgcgttc
    23461 cgcaaacacg cgcaccgtgc cgtcctcttc cagccggtcg gcaaagaaac ccgatgcgcc
    23521 gcccgccctg gcgcgccctt cgtgttcgaa acgcactgcg ccgacggtca ggtgcacttc
    23581 gtcccccgct tccgcctgcg acgaggaaat cgaatacagg cgcggcgcaa gcgggcgcag
    23641 caggccggcg aattgttccg ccgtcagttt tgccgggaag cggtgcagca caccggcaat
    23701 cggcgtgctt tgcacaaagc cttgcaaaac ggcgttgtcg gcagcaatac ggtcgagttc
    23761 gtcattatcg gcgaacgtgg catagccttt gacaaaggcg ggggtgtttt gcgtgagttc
    23821 gaaatgggat aacagtgcgg aggcaaccgg cagggttttt ccgcccgcct gtatttccgt
    23881 tgccggattg atgccgagca ggtctaggat ttccccgacc agtgccggat cgttgtcaaa
    23941 ccaaacgccg agcgcgtcgc ccgggaggta gtgcaaatcc gaaccgctca aatcgatttc
    24001 gatgtggcgc acgtctttat cggattggcg ggcggtgatt ttctgattgg ccagcagggc
    24061 ggcgggaaag gggtctgcct tgcagtacct gccctccgat gccgtctgaa ggccggcggg
    24121 gggacgttgc ctgcggcgcg ggcgttgccc ggttttttgc ggcttcttct tttaagcgcg
    24181 cgacaatgcg gcctgtccat ccgtctgcgg cggcggcaaa gtccaaatcc gcatcaacgc
    24241 gttcgagcag gcgttttgcg cccaattctt caaaacgttt gtcgaaatct ttgcccgccc
    24301 ggcagaaatt cggataggag ctgtcgccca aacccagtac ggcaaattgg agtttgtcca
    24361 atttcggggc ttttttgcag ttcagcagtt tgtgcagcac gacggcttct tccggcggtt
    24421 cgccttcgcc ctgggtggac gtaaccagca gcaggcggcg ttcgccggcg atgtttttcg
    24481 ccttgtagcc tttcagttcg gcgcgcctga cttggatgcc ggcggctttc aggctgtccg
    24541 ccgctttgtc ggcaacggat ttcgcattgc cggtttgcga ggcggaaagg acggttacgg
    24601 aaaaaggttc tgccgccggc aatgccgtct gaagcgcggg cagccctgcg gatgccccgt
    24661 ttcctgcttt tgcccaagcg tagccggaca gccccgacac aggagctgcg tgatttcggg
    24721 cggcataggc ggtaatggcg gatttgtgtt ctgcatatcg tgttcactca taaaatcata
    24781 cctgccgcaa cagtaccgta tgtcgcttcg tctatcagga taaacgaacc ggcggcggtg
    24841 ttttccgcat aaggcgttgc cgtaacgggt ttttgaaggt tgatgcggac tttggcgatg
    24901 tcgttcatct ttaaggattc cgcgccggcc tcttgttcca gcgtgcggac atccaaaacg
    24961 ctttcaattt ccccgacttt tgccggcacg gtttgcgtgc cgtgcttgag caggtatttg
    25021 cgcgcggtgt tgagcgggcg ttcgtcaaac cagcaaagcg tggcttccgg atgtttttgc
    25081 ggggcgagcg ggaaattttt atcgacaaaa agatcgccgc gcgaaacatc gatgtcgcgg
    25141 tccagccgga tggttgccgc ctcgccggca aaagcctgcg ccacttcccc tttcggcgcg
    25201 atgatttcgg acacttcggc ggtcagcccg ttcggttcga tgcggacggt ttgcccgacg
    25261 gtgaccgaac cgcgttcgat gcgcccctga tagcctcgga aaccgtcggc cttgtcggca
    25321 tcttggcgga cgaccagttg gacggggaaa taaaaaccgg catcgcgcca ccttcctgaa
    25381 atttccgcca tatcgcccaa agacggcggc agcaaggctt ccagcctttc acgcagcagg
    25441 cgggcgagga cgggcgcgct gccggagctg gaaacggcaa tctgaacagg gtcgcggtcg
    25501 ataaccgacg ggaagatgaa gctgcaatgg tcacggtcgc ccaccacgtt gaccggcttt
    25561 tggcagcttt cggcaagatg gaaaacgcgc cggttgaggg cttggtcgct gcttgccgca
    25621 atgatgagga aaaccgtgcg gatgtgttcg gcacgaaatt ctttggcaag ccacaggatt
    25681 ttgttttccg ccgccaacgc ggagagttcg gcattcaggt gttttgcggc aaccccgacc
    25741 tctgcgcccg ccttcggcag caggctgatt ttgcgtgcgg cgaccgcgcc gccgcctacg
    25801 accaatacgg ggcggccgga gaggttggcg aaaatgggaa aataattcac tggctgactc
    25861 ctttgctgtt tgcccgcacc ttgtttccga tacggtgcgt cgcggcattt ttgtcggaat
    25921 gcgggtcatt ttaaataata aaaaggcatt tgttagaagc tgaaagctat atggggggcg
    25981 gctgcggatg cggcggtttt c
//
LOCUS       DAXDSD010000017        25871 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXDSD010000017 DAXDSD010000000
VERSION     DAXDSD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25871
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.017"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..482)
                     /locus_tag="ACFB2Q_000559"
     CDS             complement(<1..482)
                     /locus_tag="ACFB2Q_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HFC0917650.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     "
     gene            complement(572..1099)
                     /locus_tag="ACFB2Q_000560"
     CDS             complement(572..1099)
                     /locus_tag="ACFB2Q_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC0917651.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDSEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            complement(1274..1453)
                     /gene="rpmF"
                     /locus_tag="ACFB2Q_000561"
     CDS             complement(1274..1453)
                     /gene="rpmF"
                     /locus_tag="ACFB2Q_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC0917652.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            complement(1487..1987)
                     /locus_tag="ACFB2Q_000562"
     CDS             complement(1487..1987)
                     /locus_tag="ACFB2Q_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC0917653.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSDEESLDES
                     MLADEELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            2135..2725
                     /locus_tag="ACFB2Q_000563"
     CDS             2135..2725
                     /locus_tag="ACFB2Q_000563"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC0917654.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            2787..3497
                     /locus_tag="ACFB2Q_000564"
     CDS             2787..3497
                     /locus_tag="ACFB2Q_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC0917655.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(3578..5215)
                     /gene="yidC"
                     /locus_tag="ACFB2Q_000565"
     CDS             complement(3578..5215)
                     /gene="yidC"
                     /locus_tag="ACFB2Q_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC0917656.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSAPETNGLKIDKVYTFTKD
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCHIDI
                     KRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQT
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(5388..5609)
                     /gene="yidD"
                     /locus_tag="ACFB2Q_000566"
     CDS             complement(5388..5609)
                     /gene="yidD"
                     /locus_tag="ACFB2Q_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC0917657.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPVP"
     gene            complement(5674..6039)
                     /gene="rnpA"
                     /locus_tag="ACFB2Q_000567"
     CDS             complement(5674..6039)
                     /gene="rnpA"
                     /locus_tag="ACFB2Q_000567"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC0917658.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            complement(6042..6176)
                     /gene="rpmH"
                     /locus_tag="ACFB2Q_000568"
     CDS             complement(6042..6176)
                     /gene="rpmH"
                     /locus_tag="ACFB2Q_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC0917659.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            6468..8024
                     /gene="dnaA"
                     /locus_tag="ACFB2Q_000569"
     CDS             6468..8024
                     /gene="dnaA"
                     /locus_tag="ACFB2Q_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC0917660.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            8104..9207
                     /gene="dnaN"
                     /locus_tag="ACFB2Q_000570"
     CDS             8104..9207
                     /gene="dnaN"
                     /locus_tag="ACFB2Q_000570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC0917661.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(9356..11413)
                     /gene="ppk1"
                     /locus_tag="ACFB2Q_000571"
     CDS             complement(9356..11413)
                     /gene="ppk1"
                     /locus_tag="ACFB2Q_000571"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC0917662.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKTPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(11582..12025)
                     /locus_tag="ACFB2Q_000572"
     CDS             complement(11582..12025)
                     /locus_tag="ACFB2Q_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC0917663.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(12032..12548)
                     /locus_tag="ACFB2Q_000573"
                     /pseudo
     CDS             complement(12032..12548)
                     /locus_tag="ACFB2Q_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            12815..15445
                     /gene="leuS"
                     /locus_tag="ACFB2Q_000574"
     CDS             12815..15445
                     /gene="leuS"
                     /locus_tag="ACFB2Q_000574"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC0917664.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            15674..16465
                     /locus_tag="ACFB2Q_000575"
     CDS             15674..16465
                     /locus_tag="ACFB2Q_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnI domain-containing protein"
                     /protein_id="HFC0917665.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            16432..16602
                     /locus_tag="ACFB2Q_000576"
                     /pseudo
     CDS             16432..16602
                     /locus_tag="ACFB2Q_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            16595..17403
                     /locus_tag="ACFB2Q_000577"
                     /pseudo
     CDS             16595..17403
                     /locus_tag="ACFB2Q_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            17400..17955
                     /locus_tag="ACFB2Q_000578"
                     /pseudo
     CDS             17400..17955
                     /locus_tag="ACFB2Q_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18463..18618)
                     /locus_tag="ACFB2Q_000579"
     CDS             complement(18463..18618)
                     /locus_tag="ACFB2Q_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917666.1"
                     /translation="MPDRIKPSRNGGGADGIARQQGTAQTGAKPETSPTARHGDFLRP
                     LQGGDWI"
     gene            complement(18728..19039)
                     /locus_tag="ACFB2Q_000580"
     CDS             complement(18728..19039)
                     /locus_tag="ACFB2Q_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC0917667.1"
                     /translation="MKYKSGIFAAIHETMQGLHDIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(19036..19403)
                     /locus_tag="ACFB2Q_000581"
                     /pseudo
     CDS             complement(19036..19403)
                     /locus_tag="ACFB2Q_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(19789..19910)
                     /locus_tag="ACFB2Q_000582"
                     /pseudo
     CDS             complement(19789..19910)
                     /locus_tag="ACFB2Q_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(20293..20378)
                     /locus_tag="ACFB2Q_000583"
     tRNA            complement(20293..20378)
                     /locus_tag="ACFB2Q_000583"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20342..20344),aa:Leu,seq:gag)
     gene            complement(20392..20742)
                     /gene="secG"
                     /locus_tag="ACFB2Q_000584"
     CDS             complement(20392..20742)
                     /gene="secG"
                     /locus_tag="ACFB2Q_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC0917668.1"
                     /translation="MEAFKTLIWIINIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNIRQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(20749..21522)
                     /gene="tpiA"
                     /locus_tag="ACFB2Q_000585"
     CDS             complement(20749..21522)
                     /gene="tpiA"
                     /locus_tag="ACFB2Q_000585"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC0917669.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEERETGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            21705..22208
                     /locus_tag="ACFB2Q_000586"
     CDS             21705..22208
                     /locus_tag="ACFB2Q_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC0917670.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADEADWVANLANTAAVLK
                     EAFGWLWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGETVVVKDVNA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDEADALYLGELAKILERRF
                     EASSQAA"
     gene            22311..22967
                     /locus_tag="ACFB2Q_000587"
     CDS             22311..22967
                     /locus_tag="ACFB2Q_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC0917671.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFADESLQG
                     LAYADMELPLANGHKMLEPKIVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            23048..23371
                     /locus_tag="ACFB2Q_000588"
     CDS             23048..23371
                     /locus_tag="ACFB2Q_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC0917672.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(23634..25801)
                     /gene="tdfF"
                     /locus_tag="ACFB2Q_000589"
                     /pseudo
     CDS             complement(23634..25801)
                     /gene="tdfF"
                     /locus_tag="ACFB2Q_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent iron piracy receptor TdfF"
BASE COUNT         5562 a         6262 c         7630 g         6417 t
ORIGIN      
        1 atgacggcaa attgggcaag ctgttcgggt gtgcagtcga cgtttgcgcc gaggtcgagg
       61 gcgagggtaa cgtggtcggt gtcggaagga aggaatttgg cgatggcggg gcgttcgatg
      121 ccgggtatgg tcttgaggac gaaacgtgcg gttgccatga gcgcgcccgt gttgcctgcg
      181 gatacggcgg cttgggcttt gccttcttta acttggttga tggcaacgcg catggaggag
      241 tattttttgt tctttagggc ggactgaggg gattcatcca tgccgacaac ctgggtggta
      301 tggcagatgt cgatgcgttc catcggcgcg cctgccgcgt tcagggcttg gcggagctgc
      361 gtttcgtcgc cggtcataat caggcggaca tcggggtgtg cttggaggaa tgcggttgcg
      421 ccgggtacgg taacggcaag tccttggtcg ccgcccatgg catctacggc caatgtaatc
      481 ataggggttt ctccggtaga ttttcggtct gcggcagggg tcgtgcgggc ttcagacggc
      541 atcggttcgg aaaatatgcc gtctgaaagg gttagcctcg gccggcttcg gcaaggcggt
      601 tgtgttcgtc gatgtcgagt gcgtcccacg gatagttgat ccaccagtct tctacggtaa
      661 tgccgctgaa atagggaatg ccttcgggga ttttgcctgc tttggctttg attttttcgt
      721 gcaggacggc gacgccgaca gtgtcgaagt cttccttgag cagttctttc aggcagaact
      781 ccatggttac gcggctgtcg tccacttcat cgacgacgag cacgtttttg ccccgcagga
      841 cttcggggac ggggtctagc cattggactt ttttgacttc ttcggtaact tgtccttcgc
      901 tgtcgctgtc gtaataggcg gtggttacgg cataaatcgg aatttccaga aaacagcgca
      961 gcatgcgtgc cggaataaag ccgccgccgc cgatagcgat catggcatcg tatttgacgc
     1021 cggcgttccg gattttttct gccaatgctt tgatgacgcg gtggatgtca tcgtaggtgt
     1081 accagatttt ctgtttcatc ggaatttcct tggtggttgg ggcttttgga atattttcac
     1141 ggggtattat acggaacgct ttgcccctgt gtgttgacgt atggcgaaag tttggacgga
     1201 tggtgcgtcc aatgtaaaaa aagccagtaa ttgcatggca atgttctggc tttttcagtc
     1261 agtcgaatag ggattattcg cctttggctt tgaccacttt gcggccgcgg tacataccgt
     1321 tgggggagat gtggtgcggg cggtgtactt cgccggttgt gctgtcgaca aacagtgcgg
     1381 gcgcggtcag tgcgtcgtgc gaacggtgca taccgcgttt ggaaggggat tttttgtttt
     1441 gttgaacggc catttcaagc tcctagataa ataaactgtg tcctaattaa ctgcttttca
     1501 aacccgccaa aacagcaaag gggttgggtt tgtcttggtt ggcggattcc ggaagggtat
     1561 tgccgcagtg tccgtgtcgc ggcgaaaagg gcagggacat caggatttgg tcttctacca
     1621 atgcgcgcac gtcgagttct ttttcaatca gtatgccttc gagttcttcg tcggcaagca
     1681 tggattcgtc caaggactct tcgtcggaaa acaggacgat acggctgctt tcatcgagca
     1741 tgaacggcat gggtttgata catctctggc aaatcagggg catatcggct ttgacgttca
     1801 ggtcgaggaa caggcgttgc agccggtcgc gaccgccggt cagtgtaaac gatattttgg
     1861 tccgcctgtc ggcgggataa tcgtgcaaac tgactcgctc gtccaattct tccagcagaa
     1921 aactgccttg caggttctgc ctttcggcgg caaaaatttc cgggtcaatc aaattagggt
     1981 ctgacataaa cggggtatga tataatttag atgttctaac gtcaatattt ttaagaaaaa
     2041 tgttgcggta cgccgaaggc gggcgtccgt gtttcggcgc atggctggat tttaccgccc
     2101 cttcgggcgt gttttcaacg gtaaggagga tgggatgggt ttggaactgc ctttggtttt
     2161 gggtacgagt tcggttttcc gccgcgaaca gatggaaagg ctcggcattg cctttcaggc
     2221 ggcatccccc gattttgacg aaacgccgat gttgggggaa tccgcccctc agacggcatt
     2281 gcgccttgcc gagggtaagg cgcggtcgtt gaccgggcgt ttccccgggg cgttgattgt
     2341 cggtgcggac caggtggcgt ggtgcgacgg caggcagtgg ggcaagccga tgaaccttgc
     2401 caacgcgcaa aagatgttga tgcatttgag cggcagggag attgagtttt acagtgcggt
     2461 cgttctgcta aatacggtta cgggcaggat gcatcggcat atcgataaga cggtggtcgt
     2521 gatgaggcag ttggacgagc tgcacatcct ccgctatttg gagcgcgagc cggatgcggt
     2581 ttattgttcg tgtgcggcga aaagcgaggg cttgggcgcg ctgttgatcg aacggattga
     2641 aagtaccgat ccgaatgcct tgattggtct gccggttttc cgtctggtcg attttttgaa
     2701 aaacgagggc gtggatgttt tgtaactgcc cgtttatgcc gtctgaaggg ttcggacggc
     2761 atttttaacc gatgagtgag ggaatgatgt ctcctgtttt gtatttgatt cctacgcctt
     2821 tgggtacgcc tgacacgccg tgcctgttgc agcatgaaca acgggcggtt gtcgggctga
     2881 cggattttgt cgtggaagcg gaaaaaacgg cgcgtgcgca tttgaagcat ttggggataa
     2941 cgacgccgat acgcgaactg aatctgcaaa cgttgaatga acacacggat ttgaagactt
     3001 tgccggaatt gctgaaacct ttgcaagaag ggcgcagtat ggggattgtc agcgaggcgg
     3061 gttgcccggc tgttgccgat ccgggcgcga atttggtggc attggcgcat aaacacggtt
     3121 ttgaagtacg gcctttggtc ggcccgtcca gcctgctgct ggcgctgatg gcttcgggtg
     3181 cgaatgggca gaactttgcg tttaatggtt atctgccgtc tgaaaaaaat gagcgcattc
     3241 agagtttgaa tgctttggag caacgttcgc ggcagtgcgg cgagacgcag atttttattg
     3301 aaacgcctta ccgcaatgat gcgctgcttg ccgatgcggt ggaaaacctg catcctgaaa
     3361 cgcgcttgtg tacggctacg gatttgacgt tgccgactca gttggtggtc agtaaaacgg
     3421 ttgcggattg gcgcaggatg aaggaaatgc ccaatctgaa aaaacgcccg acgatttttg
     3481 tgatgtatgc gggttgaaga tttccgcccc gatagggggt gaacaataaa aatgccgtct
     3541 gaacaggttt cagacggcat ttttcatgct gcggcattta ggaaacgact tcgccttggg
     3601 cgcgttgttt ttcgatgctg cggttgatgt gccactgctg ggcgatggtc aggaggttgt
     3661 tgaccaccca gtagagaacc aaaccggcag ggaagaagaa gaacatgacg gagaaaacca
     3721 acggcatgat tttcatcatt ttcgcctgca tcgggtcggt cggcggcggg ttcagatagg
     3781 tttgggcgaa catcgttgcc gccataatga tgggcaggat gtagtagggg tcggcgcggc
     3841 tgaggtcggt aatccagccc agccaaggtg cctggcgcaa ttctacggag gcgaacaatg
     3901 cccagtacaa gccgatgaag acggggattt gcaacagcat aggcagacag ccgcccagcg
     3961 ggttgatttt ctcgtctttg taaagctgca tcatcgcttg ctgttgcgcc atacggtcgt
     4021 cgccgtattt ttctttgatg gtctgcagtt tgggtgcggc ggcacgcatt ttcgccatcg
     4081 aacggtagga ggcgttggtc aatggataca gtacggcttt gacgatgatg gtcaaaacga
     4141 cgattgccca gccccagttg ccgataatgt tgtgcagttg gttcaggagc cagaagagcg
     4201 gcgatgcgaa ccagtgtact ttaccgtagt cttttgccag ttgcaggttg tcggcgatgt
     4261 ttgcgataac ggatgtggtt tgcggaccgg catacaggtt gacggcagtt tcggctttgg
     4321 cgccggcttg gatagcggct aaaggcacgc tgacgcttgc gctgtacagc ttgtcgctgc
     4381 ggcgtttgat gtcgatgtgg cagtcgcctg cggcgcaaac gctttggccg cctttaggtt
     4441 ggaggatcca ggtggacatg aagtggtgtt caatcatgcc gagccaaccg gtcggggttt
     4501 tgcggatgta ttcggcctcg gatttgccgg atttcgcatc gtcgtccaag tcggagaagc
     4561 tgactttttg gaagttgcct tcaggggtat aaacaacagg gccgacgtaa gagtgggtaa
     4621 agtagccttg accctcgggt tcgctgtggt cgcggacgat gcggtagtcc gcgctcaggt
     4681 tggcggtttg accgctgccg ttggcgatgt cgaagcggac gttgaccaga tagctgtctt
     4741 tggtaaaggt atagactttg tcgattttca gtccgttggt ttcgggcgcg ctcaggcgga
     4801 cttcgactgt gtcgccgttg agggtgtact gttttttcgg tgcgctaaag ccgatgcctt
     4861 tcagaatgtt gttgccctgc gcgtccaaaa gttcggattg ggcgacgtag gtgtattctt
     4921 tgccgtcgcc aaacaggacg aacggtttgt tttcgtcgcc ggttgctttg tatttgagca
     4981 gggtcagccg gcgcaggtcg ccactttttt catcaataac ggcttgaacc gtgtcggtcg
     5041 ttacggtaat cggcgttgcg ggcgcgagcg cggcttcggc ggaagcggtt gctgcctgtt
     5101 tttgtgccgc ctgttggggc gcggggacgg gtttcggggt ggggaacatt ttttcccagc
     5161 cgatcataat caccagcgcg atggcgaaaa acgccgtgag tcttttaaaa tccatataga
     5221 gtttcctgaa tggtcggaaa tgccgcaggt tgcgtgttcc gatagacggg gaataagggc
     5281 gggcttcata cccgacggcc gtgccgccag cccgaaggtt cagacggcat tatatagaaa
     5341 ccgatgggaa ataaaaggaa agcgtgcaat ttgaatattg cgtcgggtca gggaacgggg
     5401 tcgtgtccgt gtccgccgaa agggtggcag cgtgcaatgc gcttgatggc gagccggagg
     5461 cctttgaatg cgccgtattt tttgaccgct tcgaccgcgt attgcgaaca ggtcggcgta
     5521 taacggcagc gcggcggaat cagcgggctg atgcagtatt ggtaaaacct tatcagcccc
     5581 agcagcagtt tggacaatag gaagttcatc gggatgttcc ttgtatggga aacccgttgc
     5641 cgtctgaacc ttgcctgcag ggtaccgttc tgatcatacc tgtttcccgc atccggttgc
     5701 ggggttgccg aacatgagtt gtgccagttc cgcccttgcc tgttttgcgg tagccctgtc
     5761 gaatttccgg cggacgcgca cgacgaaatc ctgaggcggc agccggtttt tgttcaatct
     5821 gaaccagtcg cggatgacgc gtttcatata gttccgctcg ttggcgcgtt tggcggtttt
     5881 tttgccgacc accagaccga tgcggggatg gtccagcccg ttgccgtttg agcgcgaaac
     5941 ttgcagcagg tcgcggctgc ggcggtttct gaatgcaaaa acggatgaaa aatcatccgt
     6001 ttttaacaag cggtactgcc ttccgaagcg gtagtccaaa attacactgc caggcgtttg
     6061 cggcctttgg cacggcgtgc ggccaatact gcgcgtccgc cgcgtgtttt ggagcgcacg
     6121 aggaagccgt gggtgcgttt gcgtttggta acggaaggtt gataagtgcg tttcatgatg
     6181 gttcctaaaa tagaatgggt agataattaa accgtgaatt acactccaat ttgccgcttt
     6241 tgtcaatcaa tatagaaact ttgcaggagg attgcccgat tgcctgcgga tttctgccgc
     6301 ctttctgtgg ataaattttt gcacgggttg tggataaaat atcggcgagt cggtataatc
     6361 ggttcgctgc gttttgaacc gacgcgtatt caacagattt gttttctttt tgaaaatatt
     6421 atattttctt tgttttcgat ttcattttta ccgattcgag cctatccatg acattagcag
     6481 agttttggcc gctgtgcctc cgccgtcttc acgatatgtt gcctcacggg cagtttgcgc
     6541 aatggattgc gccccttacg gttggtgagg agggtggcgt atgggtggtg tacggcaaga
     6601 accagtttgc ctgcaatatg ctcaagagcc agtttgccgg aaaaatagaa gcggtgaggg
     6661 aagagttggc tgccggccgt cccgccttcg tattcaaacc gggagaaggc gtgcgttatg
     6721 agatggcggc ggttgaaggt gctgtcgaac ctgccgagcc gtccttgcac gcggggtcgg
     6781 aggagatgcc cgtgcaggag gttctgttgg acgagctgcc gtctgaaaag cctgtcaaac
     6841 ccgctgcgtc gaaaacggcg gcggatattt tggcggaacg tatgaaaaac ctgccgcacg
     6901 agccgcgtca ggctgccggg cctgcttccc ggccggaatc ggcggcagtt gccaaagcgc
     6961 ggacggatgc gcagcgtgat gcggaagaag cgcgttacga acaaaccaac ctgtctccgg
     7021 attacacgtt tgatacgttg gtagaaggta agggcaaccg ccttgcggcg gctgcggcgc
     7081 aggcgattgc ggaaaacccg gggcagagtt acaacccgtt cttcctgtac ggcagcacgg
     7141 gtttgggcaa aacccacctt gtgcaggcgg tcggcaacga gctgttgaaa aaccgtcccg
     7201 atgccaaagt gcgctatatg cattcggacg actacatccg cagctttatg aaggcggttc
     7261 gcaacaatac ctacgacgtg ttcaagcagc aatacaagca atacgacctg ctgattatcg
     7321 acgatattca gttcatcaaa ggaaaagacc gtacgatgga agaatttttc tatctgtaca
     7381 accattttca caatgagaaa aaacagctca tcctcacttg cgatgtttta cccgccaaaa
     7441 tcgaaggtat ggacgaccgc ctcaaatccc gcttttcgtg ggggctgact ttggaactcg
     7501 agccgcccga attggaaatg cgtatcgcca ttttgcagaa aaaggcggaa gcggcgggca
     7561 tcagtatcga agacgaagcc gcgctgttca ttgccaatct gatccgttcc aacgtgcgcg
     7621 aactggaagg cgcgttcaac cgtgtcggag cgagcagccg ctttatgaac cgtcccgtca
     7681 tcgacatcga tttggcgcgt accgctttgc aggacattat tgccgagaag cacaaagtca
     7741 tcaccgccga catcatcatc gatgcggtgg cgaaatatta ccgcatcaaa atcagcgacg
     7801 tactcggcaa aaaacgcacg cgcaacattg cccgtccgcg tcaggttgct atgagcctga
     7861 ccaaagaatt gaccactttg agcctgccgt ctatcggcga ttcgttcggc ggacgcgacc
     7921 atacgaccgt catgcacggc atcagggcgg tggcgaaact gcgcgaggaa gaccccgagt
     7981 tggcgcagga ttacgagaaa ctgctgattc tgattcaaaa ctgaccggac acgcctttca
     8041 gacggcatga cattgaccat gccgtccgaa gggtaggaaa tccaaccgat ttaaggagcg
     8101 aaaatgttga ttttacaagc cgagcgcgac agcctgctca agccgctgca agccgttacc
     8161 ggcatcgtcg aacgccgaca caccctgccc atcctgtcca atgtgctgat tgagggcagg
     8221 ggcggtcaga ccaaactctt ggcaaccgat ttggaaatcc aaatcgacac cgcgggtccc
     8281 gagggaggtg cgggcgactt ccgcatcact accaatgcca agaaatttca ggacattttg
     8341 cgcgcgctgc ctgccggtgc gctggtgtcg ctggattggg acgacaaccg tctgacgctg
     8401 aaggcgggca aatcgcgttt tgccctgcaa accctgcctg ccgccgattt tccgatgatg
     8461 aatgtcggcg aggacatcag cgcgactttc tcgctggggc aggagcgttt caaaaccatg
     8521 ctgtcgcaag tgcagtacag catggcggtg caggacatcc gctattatct caacggtctg
     8581 ctgatgcagg ttgagggcag ccagttgcgc cttgtggcga ccgacggaca ccgccttgcc
     8641 tatgcagcct gcgcgattga tgcggatttg ccgcgcgccg aagtgatttt gccgcgcaaa
     8701 acggtgctgg aactgttcaa actgttgaac aaccccgacg atccgattca aatcgagctg
     8761 ctggacaagc aggtgcgttt ccaatgcaac ggcacgacca tcgtcagcaa ggtcatcgac
     8821 ggcaaattcc ccgatttcaa ccgcgtgatt cctttggaca acgacaagat tttcgtgttg
     8881 tcccgtgccg aacttttggg cgcgctggaa cgtgtgtcca ttcttgccaa cgaaaaattc
     8941 cgtggcgcgc gcctgttcct gcaacccggc ctgttgagcg tcgtgtgcag caacaacgag
     9001 caggaagaag cgcgcgaaga aatcgaaatc gcctatcagg gcggcgaact cgaagtcggt
     9061 ttcaatatcg gctatttgat ggacgtgttg cgcaacatcc attccgacga tatgcagctt
     9121 gccttcggcg acgccaaccg ctcgacgctg tttaccgtgc cgaacaatcc gaatttcaaa
     9181 tatattgtga tgccgatgcg gatttgacgg ttttccggaa cacgatgccc gtattggaga
     9241 tatgccccga accgtgcaga cggattcggg gttttgttcg gctgccggaa aggcaatgcc
     9301 gtctggaatg cggcggattg gggttgggag cgtatggggg aagtgcttgt gcgggtcagc
     9361 ctcggagcaa atcccataaa tcgttttgca ggtcggcttc gctttcgcct tcggcgggcg
     9421 cggcgcggat atagccgccg tcgggctgca tcagccacgc gtgggtattg tctgccagtg
     9481 ccatttccaa accttcgcgg atgacgcgtt ttttgagttc gggcgcggta atcggggtgg
     9541 cggtttcgat gcggcggaag aaattgcgcc ccatccaatc cgcgctggaa ataaaggtgt
     9601 cgtccgcgcc gttgttgtgg aaacaatata cgcgcgcgtg ttcgagctgc ctgccgacga
     9661 tggagcggac gcggatgttt tcggacaagc cttttacacc cgggcgcaag gtacacatac
     9721 cgcgcacaat caaatcgatt tgcacgcctg ccgcgcttgc ccgatacagg gcttctatga
     9781 cagtcggttc gatgagcgaa ttcatcttgg cggtaatccg cgccggtttg ccggcttttg
     9841 cgtgttcggt ttcgcgagcg atgcggccga taaccatttt gtgcagggta aacggacttt
     9901 ggtagagttt gttcagccgc ccgggtttgc ccaagcctgt gatttccata aataatgtgt
     9961 tcacgtcggc ggtgatttgt tcgtcggcgg taatgaggcc gaagtcggtg tagatgcgcg
    10021 atgtgccttg gtggtagttg cccgtgccga ggtgggcgta acgcttgagc acgccgtctt
    10081 cgcggcggat gaccagtgcc attttggcgt ggactttgta gccgaacacg ccgtacacga
    10141 cgtgcgctcc cgcctcttcg agctgcttcg cccagttgac gttgttggct tcgtcaaaac
    10201 gcgccatcag ttcgacgacg acggttactt gtttgccggc gagtgccgcc ttcatcaggg
    10261 cggggacgag ttcggagcgc gtgccggtgc ggtaaatcgt cattttgacg gcaagaacgg
    10321 cgggatcggc ggcggcttcg cgtatcatat cgaccacggg atcgaaagat tggtaggggt
    10381 ggtgcagcag gatgggcgat tggcgcacca aatcgaagat cgggctgttt ttgcccaagg
    10441 ctttcagacg gcccggcgtg tgcggcggaa atttcaaatc ggggcggttg actaggtcgg
    10501 ggacggcgtt gaggcggacg aggttgaccg ggcctttgac ctgatagagt tcggcgtcgg
    10561 tcagcctgaa ttgcgcgagc agaaagtcgc ggatgtaggc gggacaggtg tcggcgactt
    10621 cgagccgcac gccgtcgccg tattcgcggt cgtgcagttc gttttgaatg gcggcgcgga
    10681 ggttttgtac gtcttcttcg tcaacggtca agtcgctgtc gcgcgtgagg cggaactggt
    10741 ggcagccttt gacgttcatg cccgggaaga gtttgccgac gtgggcgtgg aggatggacg
    10801 acaggaagac gaagccgtgt ccgccgccac acagttcgga cggcagggga acaacgcgcg
    10861 gcaggatgcg cggtgcttgg acaatcgcca tacccgaagg cctgccgaac gcgtccgtgc
    10921 cgtcgagttc tacggcgaag ttgagcgatt tgttcagcgg gcgcgggaaa gggtgggaag
    10981 ggtcgagtcc gatgggggtc aggatcggca gcagttcgcg gtcgaaatag tcttcgatcc
    11041 attttttctg cgtgcccgtc caattgcggc ggcggtaaaa atggatgctt tcccgcgcca
    11101 actcgggctg aaggacgttg ttgaacaggt cgtactggtg ccgtatcagg gagcgcgccg
    11161 cttcggtaac gtcggcgatg gtttcagacg gcgttttgcc gttgtccggc ctgcgccggg
    11221 ggtgcagctt gttttcacgc ttgagccacg ccatgcggac ttcaaaaaac tcgtcaagat
    11281 tggacgacac gatgcacagg aagcgcaggc gttccaaaag ggggacgttt ttgtcttccg
    11341 cctgtgccaa cacgcggcgg ttgaatgcca gcaggctcag ttcgcggcag aggatgcggt
    11401 tttgttcggt cataaggttc tcccaaagat ggttgttgtt cggtcggagc gggcggacaa
    11461 tgccgtctga aggcttggcg gcatctgcgc cggtttcagg cggcatgacg tgacggacgg
    11521 tctgtttcag gcatcggggc aatgtgaaaa cggataccgg cacgtgcggt atccgtctgt
    11581 ttcaaatcac ttccaacaca aaataatgac gcagtttttc gtacaccgca tcgctgacgt
    11641 tgatgcagcc gttggtcata atcctgtcgg acacggacgg ggaggcgatg cgttcgtttc
    11701 gcctttcgga cggtatctga ttccaaacgc ggtgcagggc gaaaagaaaa tcgccctcct
    11761 gcttgaaacc gatgacttcg ccgccgtaac cgggcttgtc ggtactgttc agcgtcagtc
    11821 cgaacgtccc cttgggtgtg gcagtgccga tgaggacggg gtggcactga cggtcgtcgg
    11881 caaaacagag ttccgccttg gatgtgtcga cgatgacttt tttcttttga atataggcac
    11941 tgaccgcatc cggctgcccc tgtgcgaata cgggggcagt cgtcagcagg cagcacaata
    12001 tccctgaaag caggcggggc ggcatagcgg cttattggcg gatacgtttc ggttttgccg
    12061 gcacgatttc gcggatgatg acttgcggct cggctttggg tgcgggcggc gggcagacgg
    12121 catctttcgg gaagacgggg ttccagtaga agctgcgggc aaatttgtct ttatcgaaaa
    12181 tcactttgta ttggcaggta gtaacgcctt ccacgccgga agtgttttca gggtcgatac
    12241 ccacgcccgg ggtgtggaag tggaacaggt aatcccattc gcgcacgccg tacatacctt
    12301 cgtcgtaatg cgggcggccc gggattttgt agatgtcgtc tttggtcaga ccggggcgca
    12361 tctgatccag ttcgtcataa gtcgggaatg tgccgcgctt gttgtcgagc gttacggaat
    12421 agggttccgg gaaaaccgga ttgtcggtcg tgccgtcggc tttgacgttg ctttatggtt
    12481 gcgcaggcag acagaatgcc tgccgccaat actgccaagc tcggtttgac gatttgtttg
    12541 attttcatat gcagaatcct tttttacccg atgcccgtct gccttttgtt cggaagagcc
    12601 gcatcaggag agttttaaac gtttgatttt ggttcgtaat attagcataa aaaacgtgcg
    12661 tatcagtaaa cgcggtgtat ttgtacggca tacggaatga tgcgcgtgcg aatttacgca
    12721 tcctgccggc aatttgccga ttcgccgaca tcggcaacct gttataattc ctcctttaaa
    12781 ttcctaacgt tttcaagcga aaaacaaaat gaccatgcaa gaacattacc agcccgccgc
    12841 catcgagcct gcggcgcaga aaaaatggga cgacgcccgt atttccaacg tctccgaaga
    12901 cgcttccaaa cccaaatatt attgcctttc gatgttccct taccctagcg gcaagctgca
    12961 tatggggcat gtacgcaact acaccatcgg cgacgtattg agccgcttca aacttttaaa
    13021 cggcttcaac gttatgcagc ctatgggttg ggacgcgttc ggcatgccgg cggaaaatgc
    13081 ggcgatgaaa aacaacgtcg cccccgccgc ttggacctac gacaacatcg aatacatgaa
    13141 aacccagctc aaaagcctgg gttttgcggt tgactgggaa cgcgaagtcg ccacctgcaa
    13201 acccgaatac taccgctggg aacaatggct gtttaccaag ctgtttgaaa aaggcatcgt
    13261 ctatcgcaaa aacggcacgg tgaactggga cccggtcgac caaaccgtcc ttgccaacga
    13321 gcaagtcatc gacggacgcg gctggcgttc gggcgcgttg atcgaaaaac gcgaaatccc
    13381 gatgtattac ttcaaaatca cggattacgc cgaagagctg ctcaacgatt tggacaagct
    13441 ggaacactgg ccggaacaag tcaaaaccat gcagcgcaac tggatcggca aatctcgcgg
    13501 catgaccgtg cgcttcgccg tttcagacga cagcaagcaa ggtttggaag gcgattacgc
    13561 gaaattcctg caagtttata ccacccgccc cgacacgctg atgggtgcga cttatgtcgc
    13621 tgttgccgcc gaacatccgc tggcaacagc cgcagccgcc gacaaacccg aattgcaggc
    13681 atttatcgcc gaatgcaaag ccggttcggt tgccgaagcc gatatggcga cgatggagaa
    13741 aaaaggcgtg ccgaccggcc gctacgtcgt caacccgctc aacggcgaca agctggaagt
    13801 gtggattgcc aactatgtct tgtggggcta cggcgacggc gcggtgatgg ctgttccggc
    13861 gcacgacgaa cgcgatttcg agtttgccgc caaatacaat ctgccgaaaa aacaagtcat
    13921 tgccgtcggc gacaacgcat tcgacgcaaa ccgatggcaa gaatggtacg gcgacaaaga
    13981 aaacggcgta ttggtcaaca gcggcgactt ggacggcttg gattttcaga cggcatttga
    14041 tgccgttgcc gccaagctgc aaagccaagg tgcgggcgaa ccgaaaaccc aataccgcct
    14101 gcgcgactgg ggcatttcgc gccaacgcta ctggggctgc ccgattccca tcgtccattg
    14161 cgaaaaatgc ggagacgttc ccgtccctgc cgaccaactg cccgtcgtcc tgcctgaaaa
    14221 cgtcgtaccc gacggtatgg gttcgccgct ggcaaaaatg cccgagtttt acgaaacttc
    14281 ctgcccgtgc tgcggcggcg cggcgaaacg cgaaaccgac accatggaca ccttcatgga
    14341 gtcgagctgg tacttcttcc gctatatgtc gcccaagttt tcagacggca tggtatcggc
    14401 agaatccgcg aaatactggg gcgcggtcga ccaatacatc ggcggcatcg aacacgcgat
    14461 tttgcacctc ctgtacgcgc gcttcttcac caaactgatg cgcgacgaag gtttggtcaa
    14521 tgttgacgaa ccgtttgaac gcctgctcac gcaaggtatg gtcgtctgcg aaacctacta
    14581 ccgcgaaaac gacaaaggcg gcaaagactg gatcaacccc gccgatgtcg agctgacttt
    14641 cgatgacaaa ggccgccccg tttccgccgt cctcaaagcc gacggactgc ccgtcgtcat
    14701 cagcggcacg gaaaaaatgt ccaaatccaa aaacaacggc gtcgatccgc aagaactgat
    14761 taacgcctac ggcgcggaca ccgcccgcct gttcatgatg ttcgccgcac cgcccgaaca
    14821 gtccctcgaa tggagcgaca gcggcgtcga aggtgcacac cgcttcctgc gccgtctgtg
    14881 gcgtaccgtt tacgaatacc tgaagcaagg cggcgcggtc aaagcatttg caggcaacca
    14941 agacggtttg tctaaagaac tcaaagacct gcgccacaaa ctgcattcca ccaccgccaa
    15001 agtcagcgac gactacggcc gccgccagca gttcaacacc gccatcgccg ccgtgatgga
    15061 actgctcaac caatacgaca aaaccgacac cggcagcgaa caaggccgcg ccgtcgccca
    15121 agaagtattg gaagccgccg tacgcctgtt gtggcccatc gtgccgcaca tctgcgaaac
    15181 cctgtggagc gaattgaacg gcgcgaaact gtgggaagca ggctggccga cagtcgacga
    15241 agccgccctg gtcaaatccg aaatcgaagt gatggttcaa gtcaacggca aactgcgcgg
    15301 caaaatcacc gtcgccgccg acgcctccaa agccgacctc gaagccgccg cactcgccaa
    15361 cgaaggcgca gtgaaattca tggaaggcaa gcctgcgaag aaaatcatcg tcgtaccggg
    15421 cagactggtg aacatcgtcg tctaaaccgc ttttaaggtt tagccatacg gataaaggcc
    15481 gtctgaaact tggaaacagg gtttcagacg gcctttttaa ggcagattgg gttgtctgca
    15541 agacagacct caaatatagc ggattaactt taaaccggta cggcgttgcc ccgccttgcc
    15601 ctgctgtctg cggcttcgtc tccttgtcct gatttttgtt aatctactaa aaattaatct
    15661 caaaatccaa aatatgaatt tatttttcga tacccaattg ggaaagcaac aaaataaagc
    15721 aacccacaaa atccgtgtaa tgagcgaggc ttggctggaa aaaaacggct attgcccctg
    15781 ttgcggaagc aagccgatgc agagatttgc caataacaaa cctgttgcag acctcttttg
    15841 cccaaattgc cacgagcaat atgaattaaa gagtaaaaat caaaaaacca taggcaacag
    15901 cgtgcctgac ggtgcatatc gcactatgtt ggagcgcatc cggtcagata ccaaccccaa
    15961 ctttttcttt cttgcatata aaaaagcgga ttactccata cggcaattgg tgcttgtacc
    16021 caaacatttc atcacgccgg atatgattat tcccagaaat aaaggcatta aaaaccgacc
    16081 gcaccacatt atgtgttcca tcaatctcgc ccctttgcct gaaagcggaa aaatattctt
    16141 aatagacgat tcccgcatta tcgaacccga aaccgttctg aaaaaatggc aatccaacct
    16201 gtttttacgc aaccaaaatg cggagcgcaa agactggctt ttggctgtta tgaaatgtat
    16261 cgaccaactc accgaagaat tcacattgtc gcaaatgtat gaatttgaaa acaaactatc
    16321 catccaattt ccccaaaaca accatatcaa agacaaaatc cgccaacagt tgcaaatttt
    16381 gcgtgatcaa aatatgatcg aattcattgg tcgcggactt tacaaaaaaa tcgacaaatt
    16441 gcacccaact cccaaggcgt tttgatttca aatcatgata ctgaatttac gagaaatatt
    16501 tattcttctg ccattttaaa aacggttgaa gtacaacgca atattgccgc aaagggcagc
    16561 agtcgtaaaa aagtcggaga attattggca atttatgatt gattttgaca aaccggctga
    16621 agaagctgcc atttatcaaa gtcgtttgaa aaaatcgttt cagacgacct tgtcctctac
    16681 aacgaaaatt cccttaacgt catgcggaag atattggaaa aacatccaaa cggctgtttt
    16741 gatatgattt tcgcagaccc gccttacttt ctttccaatg acggtttcag ttgtcaaaac
    16801 gggcaaatgg tttccgtcaa taagggcaac tgggataaat ccaaaggaat ggcggcagat
    16861 ttggaatttt acgaagaatg gctccgactg tgttacgcct tattaaaacc aaacggcaca
    16921 atttgggttt gcggcacatt tcataatatc tatttaatcg gctacctgat gcaaaccgtc
    16981 ggctaccata ttttgaacaa tattacttgg gaaaaaccca atcctccccc taatttgtcc
    17041 tgccgtttct ttacccattc gacagaaaca atcttatagg caaagaaaaa taaaaaagct
    17101 aaacacacgt ttcattatga aaccatgaaa acacaaaatg atggcaaaca gatgaaatgt
    17161 gtttggacat ttcctccacc aaataaaacc gaaaaaacat tcggcaaaca tccgacacaa
    17221 aaaccactcc ccttacttga acgctgcata ctatcggctt caaatatcgg agatttaatt
    17281 tttgaccctt ttatgggcag cggcacaaca ggcgttgccg ccttaaaaca tggtcggaga
    17341 ttttgcggtt gcgaactgga agaagatttt tttgaattag caaagaaaag gttagaaaaa
    17401 tgattattgg cggaatcggc ggtgcaagga cacaaacagg actcagattc gaagaacgta
    17461 cagacttacg aaagttgttt gaagaaattc ccgggtacga cttaagaaaa acagatgata
    17521 atgcgggtta tgaagtttgg tttaatggag aattgaaggc ttattgtttc aaaaaatatg
    17581 agttttaccg atttttggaa agactggaat acaatattaa ttggaaagac catctgtcta
    17641 aaagattact gcccgataat ggcttattta tcatcatccg tgatacctta tttattattg
    17701 agatcaaatt ccaacaaact cccggttcag tagatgagaa attacaaact tgcgatttta
    17761 agagaaaaca atatacaaag ttagttcact ctttaggttg gcgggttgga tatgtctatg
    17821 ttttgaatga ttggtttaca aaaccggaat attaaagatg ttttagatta tattatcagt
    17881 gttaactgcc attaccaatt taacacgatt cccttaaggt ggtttggact gcctgatggt
    17941 gaaacaaatg aataaatacc atatttaaaa cacaaccgtt caaggtcgtc tgaatatcat
    18001 ttcagacgac ctttcaccaa tccccgccgt tttccatctt tctgctattg tgataaagta
    18061 gcccgacctt ttgttcaaaa tacgcggatt ccgcgacact gaatgcagca tctgtcaaag
    18121 attatttgaa aacggtcgag ttaaacaagt ctgccgacaa tgtcgatacc acatccaaaa
    18181 ctatccgcag ggtttaggcg gtattccaga gtacggtgtc ttatgctgat gctgtaaaca
    18241 atgcctgtat agaaaatcta aaacggttgt tgccgaccgt tatgccggtt tatgagcaaa
    18301 gtgtcagaaa taaggggcgc gttaataaaa aacgtcggcg ttaagggaag gggatcgaga
    18361 atttgagccg ttgtttcaaa atgccgtctg aaatcgtttg agattcagac ggcatttaaa
    18421 cagttctgcg ccccgcccgt tttgccttca agcccgcgcc gcttaaatcc aatccccccc
    18481 ctgcaacggg cgcaaaaaat ccccgtgcct cgcggtcggg gatgtttcgg gtttcgcgcc
    18541 cgtctgcgcc gtgccttgct gtcgggctat gccgtctgca ccgccgccgt ttcggcttgg
    18601 tttgatgcgg tcgggcattt atgcggctac gccctgatat tcagttcggc ggtcttgcct
    18661 gttttggcag gcatattcag cagcgcgtct attgtgaact tgctgatttt gccgtttgcc
    18721 aagtcggcta cgcaatggct tcgatgccct tgtttttgac gatggtcagc agcttcaacg
    18781 ccgcgccgct cggcttttta acgccccgct cccaagccga aacgtgattt ttccccacgt
    18841 tgagatagat ggcgaaagcg gcttgcgata gtgcctcctt ctccctgatt gccttgatgt
    18901 ccccgccgct caacggcttg atttcggtca ggcaggactt gtcaaagccg cgcatagtct
    18961 ttttgtcgat tgcgccgatg tcgtgcaatc cctgcatggt ttcgtgtatt gctgcgaaaa
    19021 tcccgctttt atatttcatg gctgtatctc cgtgaaagtg ccgtttgact tggcttgctc
    19081 tatgtcggtt tctgtcattg ccaagatgat ggcggcggtt ttctttaatt ctgtaagttc
    19141 tttatcagtg atgttttcgc ggtcgttctt ggcaaaggcg gtcatgaaaa acgcccgttc
    19201 tccatgcctg aaaaatatca gactgcgata accgccgctt ctgccttgcc cttgccttgc
    19261 tatgcgctgc ttaatcacgc gccgcccaaa tctgcatcta tcagcccgtt atctgcccgc
    19321 tctaccgcct ctatcagctc tgaatcactg attttgtgct tcttggcaaa tttcactatc
    19381 cattggtttt tgaatatccg cattgtgcaa ccgtttactt agtgatactg gggattatac
    19441 caaagacagg caacacggca agccgccttt ttataccctg caatttctcc gtttatgccg
    19501 cctgaaacac cggcggctaa tttgattatt ttttaatcaa tcgcttttaa tggctcaaat
    19561 tgcgttttta gcgcgtttcg ctgtcttcct atgccccgca atataaccaa ccggtttaac
    19621 cgccattcct gctcaaaata ggcgcggttc cgtgcgtttt ggcttttcat tccgctacgc
    19681 tcccactgcc aagacggtaa aaaaccggaa aaccccaaaa gttgttaccg attcgttacc
    19741 atttgttacc gcatttgtta ccaatttttt atctttttct tatttttata gtgaattaaa
    19801 tttaaaccgg tacggcgttg cctcgccttg ccgtactatt tgtactgtct gcggttcgcc
    19861 gccttgtccc gatttaaatt taattcacta tatttcatat acttatttat ctaacttttc
    19921 cccggtaaca aggtaacaac taagggtatc gcttcctagt ataggttttt tccaaaaacc
    19981 caaaaaaacc gctctgcatc ttggacggtt tccccctcgt tccctacggt tcgtcagtat
    20041 tccccgcaag ctcttttccg cttggcattc ctgatttggt ggtcgttcag ctttattttt
    20101 gcggtatttt tcggataccg taaaaaatca ggttagttca attagggcgg attggacggg
    20161 attgcacagt caggaagggg acggcaggat aacaatcagc ctgaaatcct tgtattgatt
    20221 gggattggtt gatggcatca gacaggatta aacgaaaaaa aacgcctaga atttctaagc
    20281 gtttttgtgt gttggtgccg acagcgagat ttgaactcgc acagcctacg gccactaccc
    20341 cctcaagata gcgtgtctac caatttcacc atgtcggcat ttgaaaaact gttatttctg
    20401 ctgctgagga acaggggcag aaggttcggt attgcttacg ggtttgggtg cttgctgagt
    20461 ctgtcgtatg ttgctgaagt ccaaaccgtg ttttgtcgtg tgggtgtgaa tatacaccat
    20521 agccatgcag gttgcgaaga aaaatgttgc tgcaacggcg gtcgagcggc tgaggaagtt
    20581 ggcgttgccg gcagagccga atacgccttg cgcgctgccg cttcccgatc cgaaggtcgc
    20641 gccggcatcc gcgcctttgc cgtgttggag caatactaac acgatgacgg ccaaagcgga
    20701 aataatatta ataatccaaa ttagggtttt gaaggcttcc atatttttct acgcattttg
    20761 tgcggcactg atgatggcgg taaaggagtc gtacgacaat gacgcgccgc cgacgagtgc
    20821 gccgtccaca taaggtactg cgaagatgtc ggccgcgttg tccgctttca cacttccgcc
    20881 gtaaaggacg cggattttaa catcgcttcc gcacaaagac aagatttctt tgtagatgaa
    20941 tgcgtgcata tcggcaatct gttcgacggt ggcgactttg cctgtgccga ttgcccagac
    21001 gggttcgtag gcgacggcga tgtttttggt atccagccct tgcaggatgg aaagctgatg
    21061 ggcgatgact tcgtgttctt tgccggtttc gcgctcttcg aggctttcgc cgacgcacaa
    21121 taatgggatg agtccgacgt tgaggacgtt ttccattttg cggcgttgga tttcgttttt
    21181 ttcgccgaaa tagaggctgc gttcggagtg tccgatgagg acgatgtctg ttccggtgtc
    21241 ggcgagcatt tcggcggaca cttcgccggt gtacgcgccg ttgtcgggga agcggctcac
    21301 gtcttgggcg caggtgagga tgcggttgtt taagacgatt tgcatggcgt tgtgcagttg
    21361 cagcaggtaa acggtcgggg cggcgagtcc gatgaggacg cgttcggcgg tggggaggat
    21421 gcggaagcgg tgcatcagtg cgttgttgtt ttggagccgg ccgttcattt tccagttgcc
    21481 gatgacccat ttttgatccc acattccgat ttggcgatac atcttttttg ctccgtgtcg
    21541 tgtttttctg tctgccgcgt gtggcgcgtt gcaatgtgaa gtttagtgga tatgcggcgg
    21601 gttcgcaact tggggcgggc ggctgcgggg gcggtttgga atgttgtttc gggcaggatg
    21661 ttttataatg gctgcctgat atgtatgcaa atatgggaga tatgatgcac gcgcttcatt
    21721 tttcggcttc ggacaaggcc gcgctttatc gtgaggtgtt gccgcagatt gagtctgtgg
    21781 tggcggacga ggcggattgg gtggcgaatt tggcgaacac ggcggcggtt ttgaaggagg
    21841 cgttcggctg gttgtgggtg ggtttttatt tggtcgatac gcgttcggac gaattggttt
    21901 tggcaccgtt tcagggacct ttggcgtgta cgcggattcc gttcgggcgc ggggtgtgcg
    21961 gtcaggcttg ggcgaagggt gaaacggtgg ttgttaagga tgtgaacgcg catcccgacc
    22021 atattgcctg ttcgtctttg tcgcgttcgg agattgtcgt gccgctgttt tcagacggcc
    22081 gctgtatcgg cgtgttggac gcggacagcg aacatttggc gcagtttgat gaggcagatg
    22141 ctttgtattt gggcgaactg gcgaagattt tggagaggcg gtttgaggct tcgagtcagg
    22201 cggcttgaga ctggcaaaac gggcgggctt cgcgtgccga agttggcgcg gcggtagtgt
    22261 ggttttataa tgcctgccat tgataaaaca attatttgac ggagcactaa atggattttg
    22321 aaaaagcgcg gttcaatatg gtcgaacagc agatccgtcc ttgggatgta ttggattttg
    22381 acgtgttgga cgctttggag gagattccgc gcgagctttt cgcggatgag tctttgcagg
    22441 gtttggcgta tgcggatatg gagctgccgc ttgccaacgg tcataagatg ctcgagccga
    22501 aaatcgtggc gcggctggcg cagggcttga agctgacgaa aaacgatacg gttttggaaa
    22561 tcggcacggg ttcgggctat gcgacggctc tgttggcaaa actggctggc cgtgtggttt
    22621 cggacgacat cgatgccgaa cggcaaaagc gcgccaaagc ggttttggac ggcttgtctt
    22681 tggaaaacat cgattatgtg caaaataacg ggttgaccga actttccgca ggtgcgccgt
    22741 ttgatgcggt ttacgtcggc ggcgcggtaa cccttgtgcc tgaagtgtta aaggaacagt
    22801 tgaaggacgg cgggcgtatg gcggttattg tgggacgcag gccggtgcag cgcgcgcttt
    22861 tgattacgcg caggggcgat gtgtttgaag agaaggtgct gttcgatact ttggtggcgc
    22921 atttggacga caaggatgcc catcctttcg acagttttaa tttttgatgt tcggattgtg
    22981 atgccgtctg aaagcgggtt tggggcttca gacggcattt tgcttggttt ttttcggggg
    23041 gtttgtgatg gatattgtgc aacttccgtc ggcggcattg aaggcgtgga tggacgaagg
    23101 gcggatgttt tgtttgttgg acgtgcgtac ggatgaagaa gcggcggttt gttcgctgcc
    23161 aaatgcgctg catatcccga tgaatctgat tccgctgcgg caaaacgagt tgccggacga
    23221 tgtgccgctt gtggtgtatt gccatcacgg tatccgcagc ctgcatacgg cgatgtattt
    23281 ggcggaggcg ggttttgaaa acctgtacaa ccttcagggc ggcatcgacg cgtgggcggt
    23341 tgaggttgac gcggaaatgg cgcggtattg aaggcggctt cagacggcat tccttaaatg
    23401 cgtgtacctc tggtgttcca taaaggtcgt ctgaaagtgc agcttctgcg aagctaaagc
    23461 gcagtatcaa cgaagttaaa atttgcctga accttaaagg cagcctgcac cccaattccc
    23521 tcgccccgtg ggagaaggct agggagaggg cggcaaactg caggtttgct tgggcggcat
    23581 tttcaatgtg caggctgctt ttagcttcgc agaaactccg ttttcagacg accttattta
    23641 aaccgatagg taaacgccgc gttcactgtc cgcagtgcgc cgtggctgtg gcggtcgggc
    23701 tgggtgcggt agtgtttgtt gaacaggttg tccacgttca gcgacagttc ggtgcgcgga
    23761 ttgaagcggt aacgcgccat gatgtcggcg acggcgtagg ctttctggcg gctgttggcg
    23821 acggcgcggg ctttggcggc ggggttgggg atgtggagcg cggctgggtc ggtgtgggtt
    23881 tcgccctgcc ggcgcacacc cgcaccgatg gtccggccgc tgggggcttc gggggctaag
    23941 tggtaggcgg tgaagagttt gaagctgcgt tcgggtacgc tgtcggggtt caggcggctg
    24001 ccgtcttggt cgcggggttt gctttggctg tagcctgcct gtatctgcca ttcgggcgtg
    24061 atgcggccgc cgacttcgat ttcccagccg tgggttttgg cttggttggc ggcgcgatag
    24121 taggtgttgc cgctctggtc gcgtcctgct gcggtggcga ggttgttttt acgggcgcgg
    24181 tacacggcgg cggatgcgtt cagacgccct tcaagccatt cgcctttgat gtcggcttcc
    24241 agattgttgc cggttacggg tttcaggtag ctgccgtgtt cgtctttttg caattgcggg
    24301 acgaacaggc tgctgtacga gccgtaaagc gacaggttgc cggtcagatc gaacacgatg
    24361 cctgtgtagg gggtgaaacg gttggcggac acataggtca tgccttgtgt gcggctgttg
    24421 tagctgcctg cgcggtagcg gctgtatctg ccgccgagta tcagcgaaag gttgtcggcg
    24481 gcgcggaaac gggtggcgag atagccgccg atttgccgcc tggtgtcgta ttgcgggatg
    24541 gtttgggcaa acgatgatgg ctgcggatag gcgcccgtgc gggaaaattc gtaggcgttg
    24601 ggaatggcgt tgggaatgat gctgcgttcg ccgtatttgt tgctggcgta tttgtagccg
    24661 ttgatacccg cgattaaatc gtgctcgcgg ccgaacaggc ggtatttgcc ggtcaatgac
    24721 atgctggcgc tgtgggtgcg cggatcggcg tgccaataac cgggaatcag gtcggtggcg
    24781 gcagtgctgt ggtcgatgga aagtacgcct gccacaccgt agggctggcg gaagcggcta
    24841 cgggtgtagt cgtattcggc tttgagtttc cagtcttggt tgaagcggtg ttctatgccg
    24901 gcgaacaggt tgagcgcacg gttgcggctg ttcgaccaat ttgtggcggg gttgtctttt
    24961 gggccgaagg cggtggcata accttggctg tcgtacacgg cgtagctgag cggcgcgtct
    25021 gcggtttctt tcgcctgctg gtagtccatg cctgcgtgga cgcgggtttg cggtgcgatg
    25081 tcgtattcca aaatgccgta gagttcggca tcgcggctgc gttcgagctg ccgccacgag
    25141 tcgccgcgtc cgaaggtgga aaccaggcgg ccgcgcagcg tgccttcggc gttcaggctg
    25201 cccgatacgt ccgcgcccag cccgaaatgt ttgcggttgc cggcttcggc gcggacttca
    25261 aacaatggct tgcgggtcgg gtgtttgcgt accagattga cggtggcaga aggctcgccc
    25321 gtgccgtccg gcagccccgc cacgccgcgc acgacttcca cgcgctcata ggcggcggtg
    25381 ttggcattgc ccgtatcggc cagcgcgtcg gcaacgggga tgccgttgat ttggtagttg
    25441 gcgatgcggc tgccgcgcgc gaacaggtag ttgtagcccg cgcggtcgga gccgggctgg
    25501 tgccggtcgc ctgcaacagg gcgcggtcga gcgttttgat gttttggtcg cgcatttgtt
    25561 gcgatgtgat gacgctgacg ctctgcggga tttcgcgcag ggtcatgggc agcccgaacg
    25621 gggtgtgcgt gccggaaacg gtgtagccgt cgttggaact cgcggtgcgg tcggcggtaa
    25681 cggtgatggt cggcaattcg gtgctttcct gcggtttggg gtcgtctgaa acagaaacat
    25741 cggcctgcgc gtacacgggt agcagggcgg caaaaagcag ggagtatttg aagcgtgtca
    25801 tggggtttcc tttgtgtgtt ggtgtgcggt ggggttgtat agcggattaa ctttaaaccg
    25861 gtacggcgtt g
//
LOCUS       DAXDSD010000018        25094 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXDSD010000018 DAXDSD010000000
VERSION     DAXDSD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25094
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.018"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            184..369
                     /locus_tag="ACFB2Q_000590"
                     /pseudo
     CDS             184..369
                     /locus_tag="ACFB2Q_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(409..2337)
                     /gene="rpoD"
                     /locus_tag="ACFB2Q_000591"
     CDS             complement(409..2337)
                     /gene="rpoD"
                     /locus_tag="ACFB2Q_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC0917673.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(2524..4296)
                     /gene="dnaG"
                     /locus_tag="ACFB2Q_000592"
     CDS             complement(2524..4296)
                     /gene="dnaG"
                     /locus_tag="ACFB2Q_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC0917674.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LGDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(4441..7191)
                     /gene="secA"
                     /locus_tag="ACFB2Q_000593"
     CDS             complement(4441..7191)
                     /gene="secA"
                     /locus_tag="ACFB2Q_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC0917675.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            7323..7745
                     /locus_tag="ACFB2Q_000594"
     CDS             7323..7745
                     /locus_tag="ACFB2Q_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC0917676.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            8028..8579
                     /gene="azu"
                     /locus_tag="ACFB2Q_000595"
     CDS             8028..8579
                     /gene="azu"
                     /locus_tag="ACFB2Q_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC0917677.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            8674..9183
                     /locus_tag="ACFB2Q_000596"
     CDS             8674..9183
                     /locus_tag="ACFB2Q_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC0917678.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     EIMAARRCK"
     gene            9217..9837
                     /locus_tag="ACFB2Q_000597"
     CDS             9217..9837
                     /locus_tag="ACFB2Q_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="HFC0917679.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            9950..11095
                     /gene="dapE"
                     /locus_tag="ACFB2Q_000598"
     CDS             9950..11095
                     /gene="dapE"
                     /locus_tag="ACFB2Q_000598"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC0917680.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAETCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            11515..12330
                     /locus_tag="ACFB2Q_000599"
     CDS             11515..12330
                     /locus_tag="ACFB2Q_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC0917681.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTLDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADMAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(12308..12865)
                     /locus_tag="ACFB2Q_000600"
                     /pseudo
     CDS             complement(12308..12865)
                     /locus_tag="ACFB2Q_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            13215..13967
                     /locus_tag="ACFB2Q_000601"
     CDS             13215..13967
                     /locus_tag="ACFB2Q_000601"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC0917682.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDVLLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            14158..14233
                     /locus_tag="ACFB2Q_000602"
     tRNA            14158..14233
                     /locus_tag="ACFB2Q_000602"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14191..14193,aa:Asn,seq:gtt)
     gene            14364..15374
                     /gene="waaF"
                     /locus_tag="ACFB2Q_000603"
     CDS             14364..15374
                     /gene="waaF"
                     /locus_tag="ACFB2Q_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC0917683.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKKLHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSRFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDNEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            15429..15875
                     /gene="smpB"
                     /locus_tag="ACFB2Q_000604"
     CDS             15429..15875
                     /gene="smpB"
                     /locus_tag="ACFB2Q_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC0917684.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            16933..18276
                     /locus_tag="ACFB2Q_000605"
     CDS             16933..18276
                     /locus_tag="ACFB2Q_000605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC0917685.1"
                     /translation="MGALELTNSLWEPRWNSNIDYLITKNAEIRFNTKNESLLVKEDY
                     AGGARFRFAYDLKDKVPEIPVLTFEKNITGTSDIIFEGKALDNLKHLDGHQIVKVNDT
                     ADKDAFRLSSKYRKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFALRLND
                     KNSDIFDRTLPRKGLWLRVIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQL
                     SIGLMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYVDSWMQYQ
                     RFRHRINTEYATERFTSKGITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGV
                     NGKFSDSENAQVNLLGSRQLQSRVGVQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGVE
                     IDGDRRVINNKTVIETQLGVAAKIKSHLTLQASFNRQTSKHHHAKQGALNLQWTF"
     gene            complement(18259..18564)
                     /locus_tag="ACFB2Q_000606"
     CDS             complement(18259..18564)
                     /locus_tag="ACFB2Q_000606"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917686.1"
                     /translation="MDPIVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKA
                     VVINAEQTIELVQAEIESAVKIGGNYTKNDRTFKIIKSAVNFPADFILLQFNLKRPL"
     gene            complement(18605..19972)
                     /locus_tag="ACFB2Q_000607"
     CDS             complement(18605..19972)
                     /locus_tag="ACFB2Q_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC0917687.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFPPEILSNEVYRYEN
                     DINSTVYRNVLARNGTIAAEHGIGIIKKQWLPAVRTPSEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(20082..20408)
                     /locus_tag="ACFB2Q_000608"
     CDS             complement(20082..20408)
                     /locus_tag="ACFB2Q_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917688.1"
                     /translation="MKKSLFAAALLSLALAACGGEKAAEAPAAEASSTEAPAAEAPAA
                     EAPAAEAPAAEAPAAEAPAAEAPAAEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAP
                     AAEAAK"
     gene            20881..21363
                     /locus_tag="ACFB2Q_000609"
     CDS             20881..21363
                     /locus_tag="ACFB2Q_000609"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC0917689.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
     gene            21450..22262
                     /gene="hpnC"
                     /locus_tag="ACFB2Q_000610"
     CDS             21450..22262
                     /gene="hpnC"
                     /locus_tag="ACFB2Q_000610"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC0917690.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(22335..22805)
                     /locus_tag="ACFB2Q_000611"
     CDS             complement(22335..22805)
                     /locus_tag="ACFB2Q_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC0917691.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            22896..24737
                     /locus_tag="ACFB2Q_000612"
     CDS             22896..24737
                     /locus_tag="ACFB2Q_000612"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC0917692.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVHHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         5701 a         5900 c         7036 g         6457 t
ORIGIN      
        1 ctgagacctt tgcaataaca taggttacta aaattttatg ctcaatctca ttctcaaaat
       61 gcaaaacttt tctgattttt cctacttttt gctcaatatt aggaaggttt taggcaattg
      121 aaaatttttt ggcgcatttt tatgcgtcaa atttcgttaa cagactattt ttgcaaaggt
      181 ctcggcctac gcgcccaaac cctcgcaact atagccgccg tccgcgaact cttaaacagc
      241 ggacaaaccc cgccgcccga ctacggcaaa cgctgcaaag cctgctctct ggtggaaatc
      301 tgtcagccgg agttgttggc gaaacgggat gggagtgtgg gatatgtgga agcgttgttt
      361 attgtgtaag aaaaaaccgc aggtatttga aacctgcggt tttttggttt acagcttgct
      421 gtcttcgctg tccaagaaac ttctcaaacg gtcgcttctt gtcggatgcc gcagcttgcg
      481 gagtgctttt gcctcgattt gacggatgcg ttcgcgcgtt acgtcaaact gtctgccgac
      541 ttcttccagc gtgtggtcgg tgttcatatc gatgccgaaa cgcatacgca ggacttttgc
      601 ctcacgcggt gtcaggcttt cgaggatttc tttggttact tcgtgcaggc tggtgtacat
      661 tgccgcatcg gccggcgcaa cattgttggc atcttcgatg aagtcgccca agtgcgaatc
      721 gtcgtcgtcg ccgatggggg tttccatcga aatcggctct ttggcgattt tcatgatttt
      781 gcggattttg tcttcgggca tctgcatcag ttcggcaagt ttggcggaat cgggttcttc
      841 gccggtttct tgaaggtgtt ggcgcgagat gcggttcatc ttgttgatgg tttcaatcat
      901 atgtaccgga atacggatgg tacgcgcctg atcggcaatc gagcgggtaa ttgcctggcg
      961 gatccaccag gttgcgtagg tggagaattt gtagccccgg cggtattcga acttgtcgac
     1021 cgccttcatc aggccgatgt tgccttcctg aatcaaatca aggaactgca agccacggtt
     1081 ggtgtatttt ttggcaatgg aaatgacgag gcgcaagttt gcctgaatca tttcctgttt
     1141 ggcggctgcg gtttcttttt cgctcgacac catatttttg ttgatttctt tcaactcttc
     1201 gatggaaatg cgggtttctt tttccatatc cgccaactcg gtttgttttt cgaggatggc
     1261 gtggcggaag cggtcgagtg cgttgctcca aaccctgcct ttggcgattt cttcttcaat
     1321 ccattgcaga ttggtgattt cgggcaggaa gttttggatg aagtagtcgc gttccatatg
     1381 gacgcggtcg aggcagatgt cgcggatttc gcgttcgagt ttgcggatgt tttctacttt
     1441 tccgcgcagg ctgctgctga ggctgtcgat ttgccgggtg gcgaaacgga cttccagcag
     1501 tttgttggca atcgcgtcgc ggtaggcgag atagtctttg tgccggctgt ggtgtttttc
     1561 caaacagccg atcatttttt tgtagtcttt ttcgatttgg gcaaagtggc cgatgacttt
     1621 ttgtttcaat tcggcgagat tggctgccga gacttcatcc gcatcttctt ccgattcttc
     1681 gtcgtcttcg ttttcgtccg aattgtcgtt ggaaggtttc tcgggtgctg tggtttccaa
     1741 gtgccccaag cccaattcgt tgagcaatac ttcattcggg tcgataatgg cttctacgac
     1801 ttcgtcgacg cggatttcgt ctttgcagat tttttcgatg agttctaaga tttccgcaat
     1861 ggaacccggg caggcggaga tggcctgaac catatttttc agggcgtttt cgattttttt
     1921 ggcgatgatg atttcgtctt cgcgggtcag caggtcgacc tgtcccattt cgcgcatata
     1981 catacggacg gggtcggtcg tcctgccgaa ctcggaatct gcgctggaaa gggcggcctc
     2041 ggcttcttcg acggcatcat cgtcggtaac ggcggcattg tcgcttaaca atatgtcttc
     2101 cgcatcaggg gcgtgttcgg taacttggat acccaaaccg gaaatcatac tgacgatgtt
     2161 gtcgatttga tcggcatcgg acatatcgtc gggcagtgcg tcgttgattt cggagtaggt
     2221 gatgtagccg cgttctttgc ccataatgat gagttggcgc aggcgcgcac gttgttcctc
     2281 aatcgtcagg gggcgggcat cgtcttggta ttcttcgtga ttttggtttt tggacattgt
     2341 ctgctttctg ttggcaagat gccgacggtt tgcgtttgga cgcacctgaa ggcgtggcaa
     2401 agtagggtac tctgatgccg tctgaaggct ttttgggatg ggtgttctgc aggtgtcgtt
     2461 gttgcagccg gggtgaatgc ttttcagacg gcataacggt ttacagatgg cggaatccgc
     2521 cggtcaattt tgttttgcgg tcagcagcga cagcaaaagt tttttctcgc tttcatttaa
     2581 gccggattgc aggctttttt gttttaatgt ttcaatttgg ctgtatttta actcattgag
     2641 cagttttttg atgccgattt ggaagttttc gcaatcttct tcgccgccgc cttccatttc
     2701 ttccgattga agggccgacc ggaagatgcg gttgattgtt tcttcgtagg gcgaaccgcg
     2761 catatgttcc aaaacctgtg cggttgccgg cacggaagga tggtttttaa tggtttcggc
     2821 gaggttggca aggcaggcga aatcgccgtc caacgccaga taatcgggca ggtctatata
     2881 tgcagcccaa tccggattta tcaagaggct gcggatttgc cgttgcacca atgtcggcat
     2941 aacgggctgt ttgacggaaa tcggaggcag tttgtagttt ttttgtttga cgtgccgctt
     3001 tggcgcttcc tgtccgagca gttgcgcgag gttgtcgggg tcgatgccga ccagctcgct
     3061 aagccgttgt tttaacaaat aagccaatgc cggtgcggta atctgcacca aaagcggcga
     3121 actggttttc accaattccg ccttgccttc ctgcgtattg agatgaatgc cgtctgaaag
     3181 gtgttcccag aaatattccg acaaaggctt gctttgattc agaagcgcgt cttcaaattg
     3241 cgctttgccg taggcgcgga tgtagctgtc ggggtcgtgt tcttccggca ggaataaaaa
     3301 atgcagcgat ttgtcgtctt tcaactgcgg cagcgcgttt tccagcgcgc gccaagccgc
     3361 ttttcgcccc gcgctgtcgc cgtcgaaaca gaaataaata ctgtccgcct gccgcatcag
     3421 gattttgacg tgttccgccg tcgtcgccgt gcccaaggcc gccacaccgt agcccacgcc
     3481 gaactgtgcc agcgcgacca cgtccatata gccttcgacc accaaaatcc gttctgcctc
     3541 ttttacagca gcacgcccct catacaagcc gtaaaggttt ttccccttat cgaacaaagg
     3601 cgtatcgggc gaattcaaat atttcggttt cgagtcgccc agcaccctgc cgccgaaacc
     3661 gatgacctgc ccgcgcggat tgcggatggg gaacataatc cgatggcgga agcggtcgta
     3721 atgccgcccc tcattgtcaa tcaccatccc cgtatccacc aacgcggtat tcggatacgg
     3781 ttggaacact tgcgccaaag gctgccagcc gtcgggcgcg tagcccaaac cataatgcgc
     3841 gatgacttcc gcgctcaagc cgcgtttgtc caaataagct ttcgcagccg gattgaattt
     3901 cagctgctgc gcgtaaaaat cagctgccgc cgccgtcgtt tcttccagcg tctgctgttt
     3961 tttcttgcgc tcggcacgga cttcgggatt atcgttctgc ccgcgcactt taggcacaat
     4021 catacctacg cggtcggcaa ggaactgaac cgcctccgga aacgacagtc cctgatgttc
     4081 catcacaaaa ccaatcgccg aaccatgcgc cccgcaactg aaacaatgat aaaactgctt
     4141 ggtcggactg accgaaaacg acggtgtttt ttccttgtgg aacgggcaac acgccatata
     4201 gttcgccccg ccttttttca gcggaacctg ctcgtcgatg atgccgacaa tatcggtttt
     4261 ggccagaagc tcgtcaatga agtcggatgg aatcatgatg tggggggacg ggatgtctgt
     4321 tttcggtacg gacggtacga aatcgggatt tttcggaatg gcggtcggat gtggcggttc
     4381 aggcaaaatg ccgtctgaag ccggaaaccg gatgttcaga cggcatttgc gttcaaacgc
     4441 ttaagccaat ttgccgtggc attgtttgta tttcaaaccg ctgccgcagg ggcaggggtc
     4501 gttgcggtgg acgatttgtc cccgcgcttc caaggcttcg gggctgaaat ccgtcccatc
     4561 gggattaaag gcttcggtaa ccagatcggt ttgcgactga cccaaaagtt cttctatatc
     4621 gggcgattcg gaatggatgg actggatgtt gccgacgggt tgctcttcaa ccgccgcgac
     4681 agggttttgt tcgatttgaa ccgaagtaag cagggaggcg atatggaatt tgatgccgtt
     4741 ccacaggtct tggaacatgg taaaggcttc gcgtttatat tcctgcttcg gatttttctg
     4801 ggcatagctg cgcaggtgta tgccttgtcg caggtagtcc atggcggcaa gatgttcgcg
     4861 ccattggttg tcgatggcct gcaacatcac gttgcgctcg aaatcagcca ttgcctgctt
     4921 gccgaccagt tcggttttgg cggcatattc gttttcgata cgttcgatca ggcgctcttt
     4981 gatgtcttga ccgtcaatcg cattgtccgc cttcagccag gattggatgt cttcctgaag
     5041 cctgaattcg gcagccaggc ggttttccag tgtcgggatg tcccattgtt cttccatgct
     5101 gtcgggcggc atataggtat ccacgaggtc gctgacggca tcagaacgga tttcctgcat
     5161 caggtcgccg atgtctttgc tggtcagaat ttcgttgcgc tggctgtaaa tgactttgcg
     5221 ctgttcgttg gcaacgtcgt cgtattccaa aacctgtttg cgcatatcga agtttctgcc
     5281 ttcgactttg cgttgcgccc cttcgatttg gcgcgtcagc aggttgtgct cgatggcaac
     5341 gccgcgttcg ggggcgaggc ggttgaggat ggcggcggcg cggtcgagtg cgaagaggcg
     5401 caacagcggg tcttcaaacg agaggtagaa acggctggat ccggggtcgc cctgacggcc
     5461 ggaacgtccg cgcaactggt tgtcgatacg gcggctttcg tggcgttccg taccgatgat
     5521 gtgcaaaccg cctgcttcca tcactttatc gtgttctgcc tgccagccgt tttcgagtgc
     5581 ggcgatttgt gcctgtttct cttcgtcgct caaggtttcg tcggcgcgga tggcatcggt
     5641 ttggtgcttc aggttgccgc ctaaaacgat gtccgtaccg cgtcccgcca tattggtggc
     5701 gacggtaatt gcgccgactt tgccggcttg ggcgacaatc agggcttcgc gttcgtgttc
     5761 tttggcgttg aggacgttgt gcggcagtcc ggctttttgc agaaggcggg agaccagttc
     5821 ggagttttcg atgctggtcg taccgacgag gacgggctgc ccgcgtttgt ggcattcctc
     5881 gatgtctttg acgacggctt cgaatttttc ttcggcggaa cggaaaatct ggtcgttgaa
     5941 gtctttgcgc tgtacggggc ggttggtcgg gatgatgacg gtttcgaggt tgtagatgct
     6001 ttggaactcg aaagcctcgg tatctgccgt accggtcata ccggagagct tggtgtacag
     6061 gcggaaatag ttttggaagg tgatggaggc gagggtttgg ttttcgcgtt tgatttccac
     6121 gccttctttg gcttctacgg cttgatgcag gccttcggac cagcggcggc cggacatcag
     6181 acggcctgta aattcgtcaa cgatgacgat ttcgccgtct tggataacgt aatgttggtc
     6241 tttgtggaac agcgagtgcg cgcgtaatgc tgccataagg tggtgcatca gggagatatt
     6301 tgccgccgag tacagcgagt cgttttctgc cagcagcccc atttgggtca ggatttgttc
     6361 ggcgtgttcg tgacctgttt cgctcaggat gacctgatgt gccttttcgt cgacccaata
     6421 gtcgccttcg ccttcttctg tctcttgacg gacgaggtgg ggcggaacgg tgttcatgat
     6481 ttggtagagc tggatgttgt cgtctgcctg accggagata atcagcgggg tgcgcgcttc
     6541 gtcaatcaag atggagtcca cttcatcgac aacggcaaaa ttcaattcgc gctgcacttt
     6601 gtcgtattgg tcggttacca tattgtcgcg caggtagtcg aagccgaatt cgttgttcgt
     6661 gccgtaggta atgtcggcag cgtaggcgtt ttgacggtcg aacggctgca tatctgaaat
     6721 aatcacgccg acggtcagac cgaggaaatt gtagagcggc tccataatgc ctgcgtcgcg
     6781 cgaggcgagg tagtcgttga cggtaacgac gtgcacgcct ttgccggcca gcgcgttgag
     6841 atagacggcg agggtggcga ccagggtttt gccttcgccg gtacgcattt cggcgatttt
     6901 gccgtcgtgc agcaccatac cgccgataag ctgcacgtcg aagtggcgca taccgagtac
     6961 gcggcgggac gcttcgcggc agacggcgaa ggcttcgggc aaaatgccgt ccaaagtctg
     7021 accgtcggcg aggcgttgtt tgaattcggc agttttggct tgcagatcag catcgcttag
     7081 ggcttgcatc tgttcttcga gcgcgttgat tctggcaacg gatttacggt attgtttcag
     7141 caagcggtcg ttgcggctgc cgaagatttt cttggcaatg tttgtcagca tgaataattc
     7201 ctgcgcctga tataatagtg gggcgaaaac gcgattttaa cacaagcgtt tgtgcaaagt
     7261 gctaatgccg tctgaaataa aggcgcgttt gcggtatttc aatcttttgg ggtgtatgtt
     7321 ttatgaattt ggaacagttg ggcaggcggg acgcgctgct ttccggactt ttgaaacagg
     7381 cgggacagtg gcggcggttg gatgctgccg tgaaaaaact gctgcctgcc aacctgcacc
     7441 cccactttca gacggcatgt atcgaagacg gcaggcttgt ccttttggcg gcaaacaata
     7501 tggcggcatc gcgtttgaag atgattgccc cgtcggtatt gccgcagttg gcagggcttg
     7561 atgcttcgat acggtctgtt tcggtcaggc tggttccaaa accggaaaaa ccgccgaaaa
     7621 ccaataccct gcatttgagt aaggctgcgt tggagagttt cgattcggcg gcggcaaagt
     7681 tggaagaacg gcatcccgaa ttggcggagg cactggagga gctggtcagg aagtacgggg
     7741 cataaggcgg ccggcaggga tgttgtaaat atccgttaat atttgcgcgg cgggggacgg
     7801 ctgccctaat attaaagaaa taggttcggg gtaagattgc cctttttttg ggtaaacgat
     7861 tgtaaacttg caaacaggct ttgatttcaa atgaagtttg tagcaaaatg ccgccccgaa
     7921 acatctgttt gtgcaacacg gcggaatctt tttcaaggtt ttgttaatgg cggttgcact
     7981 ttgatttccg taaaaccgaa tattatttta tcgattggag atttaccatg aaagcttatc
     8041 tggctctgat ttctgccgcc gttatcggtt tggctgcctg ctctcaagaa cctgccgcgc
     8101 ctgctgccga ggcaactcct gccggtgaag cacccgcttc cgaagcgcct gccgccgaag
     8161 ctgctcctgc agatgctgcc gaagcccctg ctgccggcaa ctgtgcggca actgtcgaat
     8221 ccaacgacaa tatgcagttc aacaccaaag acatccaagt cagcaaagca tgtaaagagt
     8281 tcaccatcac tctgaaacat accggtacgc aacccaaagc cagcatgggt cacaaccttg
     8341 tgattgccaa agctgaagac atggacggcg tatttaaaga cggcgtaggt gctgccgata
     8401 ccgactatgt caaacctgac gatgcgcgcg ttgttgccca caccaaactg atcggcggcg
     8461 gcgaagagtc ttccctgact ttggatcctg ccaaattggc tgacggcgac tacaaatttg
     8521 cctgcacctt cccgggtcac ggtgctttga tgaacggcaa agtgactttg gtcgattaat
     8581 ccgcttaaag tttcaaaaga cagacagcct gctttgtgca ggctgtttta ttataaaatg
     8641 actgcttgaa aagtccccgt tgagaacgaa aacatgaatc cgtttgaaac ccaaagcgtt
     8701 acctttgccg aaccgattga aatgctgtat gcctgccacg gcaaagtgcg ccgtttttgc
     8761 ggccagattg ctatgttgtc gggctatatc gccgaaaacg gctgcaatca gcttgttttg
     8821 caaaccatcc gccaaatctc ccggtatttc aacgttgccg cgccgctgca ccatgaggac
     8881 gaagaagaaa actttttccc gctgctgctg caatacgcgc cgcaagcccg tgaaggcgtg
     8941 gacgagctgt tgcgccaaca tatcgggctg tacgacaact gggcggctgt ttccgccgaa
     9001 tttgccaaac tcgaagcaga caacgcttat atccccgatg cggaagcgtt caaacgcttt
     9061 gtggagggat atgatgtgca tctggcgatt gaagagcctt tgttcgacat gggcaaaacg
     9121 tttatccccg aagagaagct gaccgaaatc ggcgaaatca tggcggcgcg ccggtgcaaa
     9181 taatgccgtc tgaaacaatc gtctcaagga tatattatgc tgacgcccaa aagctgcgat
     9241 ttgttcaata tcccgttttt ccagtttgcc cagctcaaaa aataccagcc cgagagcatt
     9301 ccgcaaatca aagccgacta caaggaaaac tggcagatat ggcagcagtt gatacagcag
     9361 gtcgccgcag atttgagcga acctttcgcc ccgccgcata tcgaacgctg gtgtaacggc
     9421 tggcaggttc gcgcccattt cttcgcctac ttcaaatacg cccaatacaa aaattccgcc
     9481 gccatcctgt cgatactgct caaccgccgc cgcctgagcg tcagcttgga ctggcattgc
     9541 tacaaagccg acgtgtcgcc gattgcgctg cccgagtaca accgctggct ggatgatttc
     9601 gataccgaaa aatacgccgc attcgatatg tggcacggcg cggaaagcga atatgacgac
     9661 taccgcaccg tcgcacaaca aagcgaatcc gaccgaaggt tgcaaaacga cgaagacttt
     9721 ttctgcatcg gcaaacacat tgagcgcgac gatttgggca agcaggatgt agcaaaatgg
     9781 atagccgaaa cggtggaaga tttactgccg ctttacgaag cctgtcacgg caaatgattc
     9841 agaaaactga cgggtacgaa tgcccgtctt atacaccaat ccaacccgga ggtcgtctga
     9901 aaatcagctt caggacattt cagacgacct gatttccaaa gtgaacaaaa tgaccgaaac
     9961 ccaatcccta gaactcgcca aagcgttgat ttcccgcccg tccgttaccc ccgacgaccg
    10021 agattgccaa aaactgcttg ccgaacgcct gcacaaaatc ggttttgcgg ctgaagaact
    10081 ccatttcggc gacaccaaaa acatctggtt gcgacgcggc acgaaagctc ctgtcgtctg
    10141 ttttgcaggg cataccgacg ttgtgccgac aggtcctgtt gaaaaatggg attcgccccc
    10201 gttcgaaccg accgagcgcg acggaagatt atacgggcgc ggcgcggcgg acatgaaaac
    10261 cagcatcgcc tgtttcgtta ccgcctgcga acgctttgtt gccgaacatc ccgaccatca
    10321 aggcagcatt gcactcttga ttacttccga cgaagagggc gatgcactgg acggcacgac
    10381 caaagtcgtc gatgtgttga aagcacgcgg agagttgatt gactactgca tcgtcggcga
    10441 accgaccgcc gtggacaaat tgggcgatat gattaaaaac ggccggcgcg gttcgctgtc
    10501 gggcaatctg accgtcaaag gcaagcaagg gcatatcgcc tatccgcatt tggcggtgaa
    10561 tcccgtgcat acttttgccc cggccttgtt agagttgacg caggaagttt gggacgaagg
    10621 caacgaatac ttcccgccga ccagctttca aatttccaat atcaacggcg gcacaggcgc
    10681 gaccaacgtc attccgggcg agctgaacgt caaattcaat ttccgcttct ccacagagtc
    10741 caccgaaaca gggctgaaac aacgcgtcca cgccattttg gacaaacacg gcgtgcaata
    10801 cgatttgcag tggtcgtgtt cgggacagcc cttcctgacc cacgcgggca aactgaccga
    10861 cgtggcacgc accgccattg ccgaaacctg cggcgttgag gccgaattgt ccaccaccgg
    10921 cggcacttcg gacggacgct tcattaaagc cattgcgaaa gaactcatcg aattaggccc
    10981 gtccaatgcc accatccacc aaatcaacga aaatgtgcgg ctggacgata ttccgaagct
    11041 gtcggcggtg tatgagagga tattggcgcg gttgttggct gaaaaggccg tctgaaaaga
    11101 agatgggctg gttgtcggat atggatgtcc gacaatctga ttagacccaa catgagtttg
    11161 agataacgca ttgattttta tgtagagcga tttatttgaa cggtaatcca ttccctctcc
    11221 cgtgggagag ggttagggag agggcattgg gacggcaacg gaaatattgt ctgtatctgc
    11281 cacgttttac cctccctccg accctcccca caggggaggg agctaagttg caggcacacc
    11341 gacggaatat ggttgaaacc ctgcccttca gggcagtaga attttggttt attttttggg
    11401 aagtaacccc ttccaaatca gggtaaacag ggctgcgcct tatgtgcggc cctgtgtgtt
    11461 gaaacattga ggaatataac cgtgccgtca aattactttc atacgcgtga acgattgggt
    11521 gctttaggcg gcatcgtgca acatcatacc gccttgtacc gcagcgtggc ggtatatgaa
    11581 ccgaccgtgg ttatcgtgcg gcgcggatgc aagaagctgc ggtgggcggg gcgcgaattg
    11641 aggattgcgg cgggtgaggc tgtcggcttg gcaggcgggc agacgcttga cgtcatcaat
    11701 attcccgatt cagacggcct ctatcaggcg caatggattg ctttcgaaca ggaaaccgta
    11761 gaaaggttcg ccgcacaata cgggacggcg caggcagtgt gcgatgcggt gaagctgccg
    11821 caccccgggc gtatgggcgc ggcgtttgat tatgcggctg cggtactggc ggatgaagaa
    11881 gtgccgcaca atgcggcgga ggccgcgctt tgcggcgtgt tggcgtggtt gcagcatgac
    11941 ggcatcggtt ttgccgtgta cggaggcgtc aacctgatgc ggcaaatccg caaactgatt
    12001 actgccgata tggctgcgga ctggtcgtcg gcaatgctgg cgcagcggct taattgcagc
    12061 gaggccgcgt tgcggcggcg gttggcgcgg caggacacga atttccgtac gctgctgacg
    12121 gatgtacgca tgatgcgcgc gctgacgctg ttgcaggtta cgcaatggtc ggtagcgcag
    12181 attgccggcg cggttggcta tgactgcccg tcccgtttca gcgcgcgctt caaagagctg
    12241 ttcggttgcg taccgtcggt ggtccgctcg gaagcggagc cggcggcata tcggcgcacg
    12301 gggcaggtca gcgtcggtgt tcgaccgtga atttcgcttc gcccaagctg tttgccttag
    12361 tggagaaacc gaccagcacg ggtgtggatt cggcggtgat gttcggcagc ttggcgactt
    12421 tcagcgcggt aagcgtgaat tcgtagcggt gtttcctgcc ttcgggagga cacgcgccgc
    12481 cgtagcccgg cgcgccgaag tccgtgcggg tttgcagcgc gcctttgggc ggcctgcctc
    12541 attgtgcggt aatgcctgcg ggcaggcggc ggacatcggc gggaatgtcg gcgaccaccc
    12601 agtgcatccg gtccagaccg atcggtgcgt ctttatcgta aacggtcagg acgaaacttt
    12661 ttgtccctgc gggcgggttt taccacgaca gcgcgggcga agcattgccg cccgaacagc
    12721 cgaagccgta aggcgcgctc aaaagctggt tttgcgtgaa gccgccgtct tcggacgggt
    12781 tgtcgaattg cagcgtgaat gcgcccccgg caaaggcagc ggttgaaaaa gccagcagta
    12841 cggcggagag gatgcgggtt tgcatagatg attcctttgc ttgatgaata agtggattca
    12901 ttataggctg tcaacggcgg caggaatgtg ccgaaacggt tggatgatgt gccggaatgc
    12961 gcaaatgcaa aaatcgtggt ttcagacgac cctttgaagg ccgtctgaaa tttttcatag
    13021 ggcgacgctt tatgtgtcgt cctgtgtgtt gaaacattcc tttccctgat gctttggaaa
    13081 tattgtatat ttaggcattg ccgcaaccag accctcaagg tttccgccgc agcctgttcc
    13141 tgtaccggcg tgcgggaatg gcggaaatcg gggatttcgc cgtcaaattt atgataggga
    13201 tacggagcaa acgaatgatt accctgcctt ccttaaacaa tctgccgtca aaatgggatg
    13261 aaatccgcca ttgcctcgaa acccgggttt ttgagtgcgg cgttatgccg tatcccaatc
    13321 tgacggaatg cgaagccggg cagttcgagc gggatttttg ggacaatatc ggctgcgcgc
    13381 ctgaagaata tgtccgtatc cgccgcgcca tccgtctgtt ggaattacgt tatcccgaca
    13441 gcttgaacga gctggtgtgt gccgccattg ccacgccttt gggcgagatg ttggcggtgt
    13501 tcggcgttaa gggattgtgc ctgctggagt ttgtcggaca gaagtatttg gaacaggaaa
    13561 ttgccgccgt ccaaaaagcc ttgcgcggac ggtttgtgtt tcgggaagac gggcggatgc
    13621 aatttttgcg tcaggaattg gatttgtatt tccaatgccg tctgaaaacc tttgccacgc
    13681 ccttggaaat gattggtacg gcgtttcaaa aacaggcgtg ggacgtgctt ttggcgattc
    13741 cttacggcga aacgcgcagt tataaggagc aggcgcagcg tttgggcaac cccaaagccg
    13801 tccgcgccgt tgccgccgcc aacgggcaga acaaggtgtc tgtcatgatt ccctgccacc
    13861 gcgtcatcgg cagcgacggc aaactgaccg gctacgcggg cgggttgaac cgcaaacagt
    13921 ttttactggc tttggaacgc ggcgaagttc agacggcatt gttttaagcg gggaaatgca
    13981 gaacggctca tcgggcttaa atatggtgtg atgacggctg tgaaaattca tacaacggca
    14041 atccttttaa ggcatcggtc ttaatgtctt tctgattcat atgcttgtcg ttttgtcgcc
    14101 catttttcca aaattaagga ttgacacatt ttttccggac gctataatac gccccattcc
    14161 ccgatagctc agtcggtaga gcgacggact gttaatccgc aggtccctgg ttcgagccca
    14221 ggtcggggag ccagaatttt gaagcccaaa cttgaatgtt cgggcttttt cacatttgca
    14281 cgtccggcgc tcggaatgcg gcgagatagg ttttccaacg gtttcgcggt aaaattctgc
    14341 ctgtctgatt tcggtttcgt tttatgtcta tcaaaatcct gattatttct cccagttgga
    14401 taggcgactg cgtgatgacc cagcccttgt tccgccgttt gaagaaactt catcccggtt
    14461 gcacgattga tgtgttcgca ccgaagtggt cgatggcggt gttcgagcgt atgccggaag
    14521 tgaatgagat tcttgaaaat ccgttcggac acggtgcgtt ggagctgaaa cgccgttggc
    14581 gggtcggcag ggagttggga cggcgcggat acgacagggt tatcgtgttg ccgggttctt
    14641 tgaagtcggc agtcatcgcg ctggcaacgg gcatcggaaa aagaacgggt tatgtcggcg
    14701 aaagccgtta ttttctgttg aacgatatac gcaggctgga taaggaacgt ctgcctttga
    14761 tggtggaccg atatacggct ctcgcgcatc agagtcagga agattttgac gggcattcga
    14821 gattccccga gttttccatt gatgaacggc ggcgggaaat ttctgtcgaa actttcggtt
    14881 tgaatcttgg aaagcctgtt ctggcttttt gcccgggtgc ggaattcggg ccggcaaagc
    14941 gttggccggc aaggcatttt gccgagttgg gcaaacacta ttcggaggcg ggttggcagg
    15001 tttggttgtt cggttcgcaa aaagataatg aaatcgccga agaaatcaac tgcctttcag
    15061 acggcatgtg tgtcaatttg tgcggcaaaa ccgatttgtc gcaggcaatg gatttgctgt
    15121 cgttggcgga cacggtcgtg tgcaacgaca gcgggctgat gcatttggcg gctgctttgg
    15181 gcaggaaggt agtggcggtt tacggttctt ccagcccgac gcatacgccg cctttgagcg
    15241 acagggcgaa aatcgtcagc ctgcacttgg aatgttcgcc ctgtttcaaa cgtgaatgcc
    15301 cgttggggca taccgactgc ctcaacaggc tgtatcccga gaagattgtg caggcggttg
    15361 aagaggcggt atgatggctt ctgttttcgg atttataccg tctgacggcg gatattgata
    15421 ggatgtttat ggctattgcg aacaataaaa aagcatttca cgattttttc attgaagacc
    15481 ggattgaagc cggtttggtc ttggaaggct gggaagtcaa agcaatccgc gccgcgcgcg
    15541 tacagcttaa agagagttat atctattgga aaaaagatgc gttttatctg gtcggctgcc
    15601 atattacggc tttgcctacg gcttcgacac acatcaaacc ggatgccgta cgtccgcgca
    15661 aactgttatt aaaacagtcg gaaatcaata agttaatcgg aaaaaccgaa cgtgcaggtt
    15721 acaccatcgt gccgctggat ttgcattttt cacgcggaaa aatcaagatg gaaatcggtt
    15781 tggcaaaagg taagaaacag cacgataaac gacaaagtat gaaggaagcc gactggaaac
    15841 gcgagaaaca gcggttgatt aagcatacgc gctgattgtg gaaacaatgc ttcagacggt
    15901 acgggcaagg ctgccgcctg atgcgtaaac gttctacacc taaaccagca tccgctttaa
    15961 tcagcacggc atgatgcccc aaaccttcgt caaaggtttg agcaatttga tgataaattg
    16021 gcattagcca agggtaaagt gcggtcattt ttcctgaaat ttttgtaaat aacgagggca
    16081 attatacaca ttttcaaacc ggcttgacaa aatagcaaaa agcaaacgtt tacttttaat
    16141 aaataagaaa taaatagaga aaatttgaac taaatgacat tttttgttca gttttatgct
    16201 aaaatccacg ccctatttat ttttacttga caaaggaaaa tttattatga aactcgaagc
    16261 aagcaagcag aagtttaaaa aatcatttat tataagtcta tttttttcta ttctttaata
    16321 cctctccgct tttggctgtt gattatgttt acgacaaaac caagctcact gatgatgaaa
    16381 ttatccgctt aaaaaaaact ccgcgataga aatagtgaat attggaaaga agaaacttat
    16441 cacataaaaa gtaacgggcg aacttatcca aacattcccg cattattccc taaacatcct
    16501 ttcgatccat tcgaaaacat caacaactca aaaaagattt ctttttatga caaggaatac
    16561 actgaagatt accttgttgg cttcgcccga ggtttcgggg ttgaaaaaag aaatggggaa
    16621 gaagaaaaac cactccggca atattttaag gactgtgtaa atactgagaa ttccaataat
    16681 gataattgta aaatttcatc cttcggaaac tatggcccaa tattaataaa gagcgatatt
    16741 ttcgccttag catcacaaat aaaaaatagc catatcaaca gcgaaatttt atctgtaggt
    16801 aattatatcg aatggcttcg cccaacactt aataagctta ctggttggca agagcatcta
    16861 tatgctggtt tagatccatt ccactatatt gaggttacag acaactcgca cgttatcgga
    16921 caaacgattg atttgggagc attagaatta acaaattctc tatgggaacc ccgttggaac
    16981 tctaatattg attatttaat aacaaaaaat gctgaaattc gtttcaacac taaaaatgaa
    17041 agtttactcg taaaagaaga ttatgctggc ggagctcgtt ttcgttttgc ttacgatcta
    17101 aaagataaag tccctgaaat accagtttta acttttgaaa aaaatataac tggcacatca
    17161 gatattattt ttgagggaaa agcgttggat aatttgaaac acctagacgg gcatcaaatt
    17221 gtcaaagtaa atgacaccgc agataaagat gcctttagac tttccagtaa ataccgaaag
    17281 ggaatttata cgctctcttt acaacaacgc ccagagggct tttttaccaa agtgcaagaa
    17341 cgcgatgata ttgcgattta tgcacaacag gctcaagccg ccaatacctt attcgccttg
    17401 cgtttgaacg acaaaaacag tgatattttc gaccgcactt taccacgcaa aggcttgtgg
    17461 ttgcgtgtga ttgacggaca ttccaaccaa tgggtgcaag gcaaaacagc acctgtagaa
    17521 ggctatcgaa aaggtgtgca actcggcggc gaggtgttca catggcaaaa cgaaagtaat
    17581 caactttcta ttggcttaat gggcggacaa gcagaacaac gcagcacttt ccgcaatccg
    17641 gatacagaca atcttacaac gggaaatgtg aaaggctttg gtgcgggcgt ttacgccact
    17701 tggcatcagc ttcaggacaa acagacaggt gcgtatgttg atagctggat gcaatatcaa
    17761 cgtttccgcc accgcattaa cactgaatat gctacagaac gttttacttc aaaaggtatt
    17821 actgcctcaa ttgaagcggg ttacaatgcg ttattggcgg aacacttcac taaaaagggc
    17881 aacagccttc gtgtttacct acaaccacag gcgcaattga cttatttggg ggtaaacgga
    17941 aaattcagcg atagcgaaaa tgcccaagtg aatttgcttg gctcccgcca attacaaagc
    18001 cgagtgggcg ttcaagctaa agcgcaattt gctttcacta atggcgtcac tttccaacca
    18061 tttgttgctg tcaattcaat ctaccaacaa aaacctttcg gtgtggaaat agacggggat
    18121 cgtcgagtga taaacaataa gacggtaatt gaaactcaat taggtgtagc agcgaaaatc
    18181 aaatctcact taaccttgca agcctcattc aaccgccaaa caagcaaaca tcatcacgct
    18241 aaacaaggtg cgttgaattt acagtggacg ttttaagtta aattgtaaca aaataaaatc
    18301 ggcagggaaa ttcactgccg attttattat tttaaaagtg cggtcatttt tcgtgtaatt
    18361 tccaccaatt tttaccgcac tttcaatttc agcttgaaca agttcaatag tctgctctgc
    18421 attaattacg actgcttttg gattatcttt tactaattct aaatagcgtg ctcgagtgcg
    18481 atggaaaaaa tctaaatcca tttgctcaat acggtctaac tcgccacgtc cacgagctcg
    18541 cgctaaaccg acaatcggat ctattgtcct tttctttgtt gtgtatgcaa atcagaaatg
    18601 ccggttacgg aagcagtttg ccaggattca taatgttata tggatcaagg tgttgtttga
    18661 tgcttttcat cagggcaatt tcagagggcg tgcggacagc gggcagccat tgttttttga
    18721 tgatacctat gccgtgttcg gcggcaatcg tgccgttgcg ggcaaggacg ttgcgataga
    18781 ctgtgctgtt gatgtcgttt tcgtaacggt aaacttcatt gctgaggatt tcgggcggaa
    18841 aagtattgta gtgcaggctg ccgtcgccca gatgtccgaa gcagacgatt tgtatgcctt
    18901 taaaattctg ttccaaatct ttggcgcacc ggcggacaaa gtcggcaacg cgcccgatag
    18961 gaacggcaat atcgtgtttg atgctggtgc ccagtttgcg ttgggaggcg gagatgtttt
    19021 cgcgcaacgc ccacatatgg atacgttctt gttcgctttg cgccaatacg ctgtcggtaa
    19081 atcctttttt ataaagaaat tcgacaagcc gatcatcaag attgctgtcg ggtaatgagt
    19141 cggtcaactc aagtaaaata tgccattctg aatgtgtcgg cagggggagt ttgctgaatt
    19201 cggaagacaa ctcggcggca aaacggccga tcagttcaaa actgcataag cgttcggcaa
    19261 agtgcgcttg ggtttcggtc agcaggcgga cggcggattc gatgtcggga atgccgaccc
    19321 atgccgttgc cttgtccgag ggacgggcaa aaagcttgag cgtggcggca gtgatgatgc
    19381 ccagtgtgcc ttcgctgccg ataaacagat ggcgcaggtc gtagccggtg gtgtttttat
    19441 gcaggggatg gagatgggaa accagttcgc cgttggggag gacgacttcc aaaccgataa
    19501 ccaggtcgcg catcgtgccg taacgcaata cgttcaaacc tccggcattg caggcgatgt
    19561 tgccgccgat ttggcacgag ccttcgctgg cgagactgag tgggaacagc ctgtttgagg
    19621 cttcggctgc ctgttggacg gtttggagta cggaacctgc ttcgacggtt atgcagttgt
    19681 ctgacaaatt gatgctgcgg atgcggttga gtttggaaag gttcagcaat acgccgtttt
    19741 ccgatactgc cgcgccgcac aaaccggtat tgccgccttg cggcgtaacc ggaatgcggt
    19801 gttcgtaaca aaaacgcata atggtttgca cgctttcaac gctgcgcggc tgcaaaatga
    19861 tgtctggtgc agacgtaaag cggttgcgct gatcttttaa aagcgtcgga gaaggttcgg
    19921 caatttcatc tgccggcagg agtcgggaaa attcggtatg aaggtcaggc atgtttgtcg
    19981 gaagggtagg gagaagattg aggaagggaa tctgatgccg tctgaaaaat caaaaaagca
    20041 ggatactgaa cgtatcctgc ttttttgcaa gcggaaaatg cttattttgc agcttcggca
    20101 gcaggagctt cagcggcagg tgcttcggta gcagcagctt cggcggcagg tgcttcggta
    20161 gcagcagctt cggcggcagg agcttcagca gcgggagctt cggcggcagg tgcttcggcg
    20221 gcgggagctt cggcggcagg tgcttcggcg gcaggtgctt cggcggcagg tgcttcggcg
    20281 gcaggtgctt cggcagcagg tgcctcggta gaggaagcct cagcagcagg agcttcagcg
    20341 gctttttctc cgccgcaggc agccaaagcc aaagacaaca aagcagcggc aaacagggat
    20401 tttttcattt tagtaacctt taaaatcaat ttaaattatt gaaacaaata ggtctttttc
    20461 gaccatacta caaaccgggt tggaaattca tcgctgaatt tgcgtaatta tgcaccatgg
    20521 aataagggat tgcaatgtcg tacagatggg aaaaaggttt gaatgaaaag ttttctggcg
    20581 ggcggattga taaatcctgt aaaacggttt tgctgttaaa aatccgatag ggataaatat
    20641 tatagagatt aatataattc agtatcttgt atgatttatt aaggcagatt ggggaagtcg
    20701 gcagatgggt cggaatggtt tttgagagtg ttgtaaaaaa tttacactgc aataaaataa
    20761 caaaaattta cattgaaata tgtgccgtac agtttatgaa caggttaacg gtaatcggcc
    20821 ggcgcggcgg ttgtgttaga atgcgcctgt aatcaatctg ctgttcctga aaggacaaat
    20881 atggctattg caaaaaattc cgtggtttcg ctgcattatg agatgtatga tgccaacaat
    20941 cagcttttgg ataaaaccga agaaccgatt gcgtatctgc acggcggtta cgacggcatt
    21001 ttccctttgg tggaagaggc gttgcacggt aaggatgccg gcgatacggt cgatgtggcg
    21061 ctttcgtccg acgatgcgtt cggcgagcag gattcggagt tggtccgtat tgaagatgcg
    21121 ggcgcgttcc ctgttgaagt cgaagtcggc atgatgtttg aagccgacga tcctgaaacc
    21181 ggcgatgttg tcgtctatcg tgtaaccgat gttgccgacg gcaaggcggt ggtggacggc
    21241 aaccatcctt tggcaggcat gaaaatccgc tttaaagcta cggttgaaag cgtgcgcgat
    21301 gcatccgatg aggaaatcgc acacggtcat gtccacggtt cgcacggtca ttaccaccac
    21361 tgattgccgg cagcttggag gtatctgatg ccgtctgaaa agggtttgtt ccgtttcaga
    21421 cggcattttg tttgatggat ggagaaggta tgtcggttgg gcattatgag aattttcccg
    21481 tcggttcgct ggttttgccg cgcaggttga ggaagccggt tcatgcggtg tatgcgtttg
    21541 cacggacggc ggacgatatg gcggacgagg gcagtatgcc gtctgaagcc aggttgtcgg
    21601 ggttggaggg tttgcggcgc gagttggacg tgttggcatc gggcggccgg tcggcgcatc
    21661 ctttgattgc acgattggat gccgaggcgg ttgtgccgtt cggtttggat ttgcagccgt
    21721 tttatgatct gctctcggcg ttttcgcagg atgtggttaa aacgcggtac gcgcatttcg
    21781 gcgatctgac cgattattgc cggcgttccg ccaaccctgt cggacgcatt atgctggctt
    21841 tatacgggaa aacggatgcg gtgtgcgtag cgcaaagcga cggtatttgt acggctttgc
    21901 aactggtgaa tttttggcag gatgtagctg tggattggca aaagggcagg gtttatatcc
    21961 cgcaggacga tttgttgaaa ttcggtgttt ctgaggaaca gatcgcagca ggacgggcgg
    22021 atgcggcgtt tcagcggctg atggcgtatg agtgccggcg tgcattccgt atgctgaagg
    22081 cgggttcgcc tttggcgcgc gaattgaaag ggcgtatcgg tttggaactc cgtatgattg
    22141 tgttgggggc gcagttgatt ttgcagaaac tggacgcgtg ccgatacgat gtgtttgcac
    22201 aacgccccgt tttggataag aaggattggt tgattatgtt gaaacgcgcg ttgtggaaat
    22261 gattggggga aaatgaatgc cgtctgaaag gggttgttcc gcttcagacg gcattttgca
    22321 ggcggtcggt atggtcaggc gatttcatgc gggtttaacc tggcttgatc ctctgcaccc
    22381 aagccgatga gtttgcgcag tcgggtcatg tagaaattga catccaaacc ggtgccgtag
    22441 cgttgcgccg tccacagtgt ttccgccagt gcctccatca tttcgtgttc ggcttccggc
    22501 cagccgcgtt tggcgcacag ggtgtcgtgg atggcgcgta tgccgtgcgg ctggtctatg
    22561 cccgcctgtt cttggacgga caaatgcagc gacatatgca ggaaggggtt gctttcgccg
    22621 ttttcgggca gccagtcggt gtccagatgg tcttcgatgc gttcgagata acggtggtat
    22681 tcgggatggg cttcgacaat gcggagggct ttctgttcca acgcgcccaa ttgcagcgga
    22741 ttgagccgct gctgccacac gcgggcgaaa aagcggcgga catcgtgggt attgacatcg
    22801 tacatggcgg gtccgataag atgtttatgg aaataggtta ggataatggc gggttttaca
    22861 taaattaaat cctaccgtca accttaccat ttataatgcg gacaaattat ttgtctgaaa
    22921 cggattcgat tatgaaaaaa ctgatttgcc tgttcgccgt atttttgatg ttgtgcggac
    22981 gggctttcgc gctggatgcg aacgatctgc tgccgccgga aaaggcattc gtgccggagc
    23041 ttactgttgc cgacgacggc gtgaacgtcc gtttcaggat tgccgacgga tactatatgt
    23101 atcaggcgaa aatcgtcggc aagaccgatc cggcggattt gttgggacag ccttctttca
    23161 gtaagggcga agagaaggaa gacgagtttt tcggcaggca gacggttcac catcacgagg
    23221 cgcaggttgc ctttccttat gcaaaggctg tcggcgaacc gtataaattg gttttgacct
    23281 atcagggctg tgccgaagcc ggcgtgtgct atccgcccgt ggataccgag tttgatattt
    23341 ccggcaacgg cacttaccat ccgcaaaccg acgaaccggc atccgccaaa gaccgttttt
    23401 tgcagccttc ctctcaaaac ggcagcgggg cgttgccgcc cccgaagggg gacgagggcg
    23461 gcgacggccg tttcaagctg tcttgggata cgctcaacgc caatcttttg gcgttttttc
    23521 tcgccggttt gggcctgagt tttaccgctt gtatgtatcc cctgctgccg attgtgtcca
    23581 gtatcgtggt cggtgacaaa aaggcgggca aggcgcgggc gtttgtgctg tccgtcgttt
    23641 atgttcaggg tttggctctg acttatacgc tggtcggcat tgttgccgga ctgacgggcg
    23701 cgctgctgac cgtatggttg cagcaggctt gggtcgtgtt ggcggcatcg gctttgatgg
    23761 tcgtcttggc gctgtctatg ttcgggctgt tcaacatcca gcttcccaac gccgtacagt
    23821 catatttcca aaaccagagc agcaggctct ccggcggtaa aatcgtttcc gtctttatta
    23881 tgggtattct gtccgcactg attgtcggac cgtgcgtcgc cccgccgctg gcgtttgcct
    23941 tgggctatat cggtcagacg ggcgatgcgg ttttgggcgg tttggcactt tacactttgg
    24001 cgttgggcac cggcgtgccg ctgattgcca tcggcacgtt cggaggacat atcctgccta
    24061 aggcgggcga ttggatgaat gccgtcaaat acgctttcgg ctttatcctg cttgccgtcg
    24121 ccgtttacct cgccacgccg catttgccct attatctcgt cgtcgcgctg tacacgctgc
    24181 tgatgctggt tcctgccttt atgctgctgg tcaacggacg caggcagaaa cgccgtccga
    24241 aagctgtggc attcgcattg ggcagcatat tgctgatagg cggcgcgtgg ttcggctggc
    24301 agggcgcaaa cggcaaaacg accgcgctgc accatttctt gaccctcaat ccgccggccg
    24361 aagcaggcaa attttcggaa cacggcaaaa tgtttgccga tactgccgcg ctgaaggcag
    24421 cgatggatac ggcgttgaaa gaacatcccg acaaacctgt cgttttggat ttttatgccg
    24481 actggtgcat ttcctgcaaa gaaatggcgg tttacacgct caatcagccg gaagtgcatc
    24541 aggcagtcga tatggaacgc tttttccaga tcgacgtaac cgccaacacg cccgaacatc
    24601 aggcgttgtt gaaagaatac ggtctgttcg ggccgccggg cgtgtttgtc gtccgcgccg
    24661 acggcagccg cagcgagccg ctgctgggtt ttgtcaaagc agacaagttt atcgagtggt
    24721 atgaacaaaa ccgctgattc cgatgtttca aatgccgtct gaagttctca gacggcattt
    24781 ttgtcagaca ggaaaacggc aatatccgct atacacacgg aagcagtcca aagatgtgaa
    24841 aaccgtgtgt ataagtcggt ttgcgtattg aaattaaaag ataaaaatac gatgattaaa
    24901 aaataggcat aagaaaagat aaacacagcc ggtgtttgcc ggtgtgtgta tcttttgtgg
    24961 aaaaacatct taatgccatg tattttatgg aaaataataa catgattaaa aaatagtcaa
    25021 tcattaaggt tcaggtaggc ggctgcaacc gtcaatgtct gaaaagaggt agtgctgttt
    25081 aagcatggaa agcc
//
LOCUS       DAXDSD010000019        24884 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXDSD010000019 DAXDSD010000000
VERSION     DAXDSD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24884
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.019"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(59..253)
                     /locus_tag="ACFB2Q_000613"
     CDS             complement(59..253)
                     /locus_tag="ACFB2Q_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC0917693.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(243..2324)
                     /locus_tag="ACFB2Q_000614"
     CDS             complement(243..2324)
                     /locus_tag="ACFB2Q_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC0917694.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            2847..3227
                     /locus_tag="ACFB2Q_000615"
     CDS             2847..3227
                     /locus_tag="ACFB2Q_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC0917695.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3363..3851)
                     /locus_tag="ACFB2Q_000616"
                     /pseudo
     CDS             complement(3363..3851)
                     /locus_tag="ACFB2Q_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            4183..5571
                     /locus_tag="ACFB2Q_000617"
     CDS             4183..5571
                     /locus_tag="ACFB2Q_000617"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC0917696.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIVKP
                     ASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNPTVRKISFTGSTEVGA
                     KIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKFRNSGQTCVCTNRVYV
                     QSGIYDVFCRKLSEKAAALKLGDGLDEGVNQGPLIEEKAVEKVEQHIADALSKGAVCL
                     TGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFETEAEVIEAANNTEYG
                     LAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGGVKRSGLGREGSKYGADE
                     YLELKYLCIDAG"
     gene            5682..6467
                     /locus_tag="ACFB2Q_000618"
     CDS             5682..6467
                     /locus_tag="ACFB2Q_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC0917697.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            6835..8391
                     /locus_tag="ACFB2Q_000619"
     CDS             6835..8391
                     /locus_tag="ACFB2Q_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC0917698.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMVVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8408..9154
                     /gene="surE"
                     /locus_tag="ACFB2Q_000620"
     CDS             8408..9154
                     /gene="surE"
                     /locus_tag="ACFB2Q_000620"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC0917699.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFCYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9256..10269
                     /locus_tag="ACFB2Q_000621"
     CDS             9256..10269
                     /locus_tag="ACFB2Q_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC0917700.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAAQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLREYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10667..11149)
                     /locus_tag="ACFB2Q_000622"
     CDS             complement(10667..11149)
                     /locus_tag="ACFB2Q_000622"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC0917701.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11302..11463)
                     /locus_tag="ACFB2Q_000623"
     CDS             complement(11302..11463)
                     /locus_tag="ACFB2Q_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917702.1"
                     /translation="MSEQPEKHHASPIEDERKKPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11537..11998)
                     /gene="recX"
                     /locus_tag="ACFB2Q_000624"
     CDS             complement(11537..11998)
                     /gene="recX"
                     /locus_tag="ACFB2Q_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC0917703.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12071..12781)
                     /locus_tag="ACFB2Q_000625"
     CDS             complement(12071..12781)
                     /locus_tag="ACFB2Q_000625"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC0917704.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLRPQKNKDEE"
     gene            13176..14395
                     /locus_tag="ACFB2Q_000626"
                     /pseudo
     CDS             13176..14395
                     /locus_tag="ACFB2Q_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
     gene            complement(14455..15261)
                     /locus_tag="ACFB2Q_000627"
     CDS             complement(14455..15261)
                     /locus_tag="ACFB2Q_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC0917705.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            15453..15662
                     /locus_tag="ACFB2Q_000628"
     CDS             15453..15662
                     /locus_tag="ACFB2Q_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC0917706.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            15751..16965
                     /locus_tag="ACFB2Q_000629"
     CDS             15751..16965
                     /locus_tag="ACFB2Q_000629"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC0917707.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(17037..19616)
                     /gene="clpB"
                     /locus_tag="ACFB2Q_000630"
     CDS             complement(17037..19616)
                     /gene="clpB"
                     /locus_tag="ACFB2Q_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC0917708.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGADEVAEIVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRL
                     KFA"
     gene            19876..20886
                     /gene="trpS"
                     /locus_tag="ACFB2Q_000631"
     CDS             19876..20886
                     /gene="trpS"
                     /locus_tag="ACFB2Q_000631"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC0917709.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQMLADGLAWGEAK
                     KLSAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            20967..21512
                     /locus_tag="ACFB2Q_000632"
     CDS             20967..21512
                     /locus_tag="ACFB2Q_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917710.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            21829..22173
                     /locus_tag="ACFB2Q_000633"
     CDS             21829..22173
                     /locus_tag="ACFB2Q_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917711.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAASAVEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAA
                     QEAADKMKDAAK"
     gene            complement(22383..23891)
                     /locus_tag="ACFB2Q_000634"
     CDS             complement(22383..23891)
                     /locus_tag="ACFB2Q_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC0917712.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGSSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIIGGNEMLWYAVLSLRLAALFCRSRQDLGFP
                     KNMQLRTDTESCGFILRIDRKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(24054..>24884)
                     /locus_tag="ACFB2Q_000635"
     CDS             complement(24054..>24884)
                     /locus_tag="ACFB2Q_000635"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC0917713.1"
                     /translation="NASSEKTIEFAGRSWTKTADSLPQKAHGLRYGLGVSREINAGGH
                     HFLYGGISRGVHYWDNKDFSEQSLRLSFGHQNRSVTRSFGTVPFVGQNLLGGSRYNFA
                     GGFNADFSRRLGERWRLTLNAGNMWKHYQEDRTAARYGSHMPLAGATLMYSAPKDRLL
                     YGGADWPHDMTKEAEQASVRKGLRVGAVKTFDGGLGLRANLRYTRRTFDAPGTIVYRF
                     PRKDHEYQANLSLRHDKISWKGFTPQLNFRYLKIDSNMKSFYTRKNTQIFMSVEKDFK
                     "
BASE COUNT         5687 a         6270 c         6903 g         6024 t
ORIGIN      
        1 ccttattata aaaataccgt ctgaagtggc gttcagacgg catttgtttc aagcggggct
       61 aacagcagcg tccgccgttt gcgccgcagc ggcgtttgcg gcaatagtct tgaaattgca
      121 gcttgttcat cacgggggcg ttgggattat gtttacgttg ttgtgcaacg tagttttcat
      181 aatcgggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac caagacgcga
      241 gcttatgctt cactttgttc ctccggttgt ttgccgtcgc ggtacaccgc cgggatttct
      301 ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc gtacgcggca
      361 acagtcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt gtcgttgaag
      421 atgatttgcg ccatttcgcc gatgtcttta gcgggcgcaa ggacttcgtt tttagccaat
      481 gcgtcgctgt atttgcctgc gtgggcaagg aagctggcgc gcgggtcgtt gtggaacagt
      541 ttttgcagac cggcgtagca ggttacaagc agtacgccga cggcaggaac gagtgccacc
      601 cagacataac ggtcgcgttt catcttaatc agcacaacgg cacacataat caaggctacg
      661 cctgccagca tttggttggc gatgccgaat aaaggccaga gcgagttgat gccgcccagc
      721 gggtcggtta cgccggtgta gaggaagtag ccccacaatg ccacggcgaa gaaggtcgca
      781 atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat gctgcccaag
      841 tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag gatgaacaag
      901 gcttcaaaca acagggcgaa gtgataccag aatgccatca tcgcctcgcc cggaatcaga
      961 cggctcataa tgtgcgccat accgactgcg agggtaggcg caccgcctgt gcgggacagg
     1021 atggtgtttt cgcccacttc cttagcggtg tgcaacaggg ttgcggcatc gacagggaag
     1081 ttcagcttgg tggtaattac ttcggcggcg gtgttggcat ccgtaccgat cagtgcggcg
     1141 gggctgttca tcgcgaaata cacgccggga tcgagcgatg cggcggcggc aagcgccata
     1201 atcgccacga aactttccat caacataccg ccgtaaccga tcatacggac gtgggtttcg
     1261 ttttccagca ttttcggcgt ggtgccggaa gaaatcagtg cgtggaagcc cgaaaccgca
     1321 ccgcaggcga tggtaatgaa caagaacggg aacaatgcgc ctgagaatac cgggcccgaa
     1381 ccgtcgataa agtgggtaac cgcaggcatt tgcaaagcgg gattgacgat gacgataccc
     1441 aaagccaagg ccgcaatcgt accgattttc aggaaggtgg agagatagtc gcgcggggtc
     1501 agcaagagcc agaccggcaa aaccgaagcc acgaaaccgt aaatcataat cgcccaagtg
     1561 agctggatgc cgtcaaggtc aaaccaatgc ccgatggagc ttttcgccac atcttcgccg
     1621 taaattaccg ccaacatcag caaaataaag ccgacgatgg aaatctcgcc gattttgccc
     1681 ggacggatat ggcgcgtgta aatacccata aacagcgcaa tcggcatcgt tgcggcaatg
     1741 gtgaacgtac cccaagggct gtgaaccaat gcttttacga caatcaacgc caaaaccgcc
     1801 ataatgatga ccataatcat caaaataccg atggaggcaa tcacgccggg gacagtgccg
     1861 agttcctgtt tcacaatatc gcccaaagac ttaccgtcgc ggcgcataga gacgaacaag
     1921 accgtcatat cctgtaccgc gccggcaaat accacgccga agataatcca caaagtaccg
     1981 ggtagataac ccatttgcgc cgccaaaacc ggaccgacca aagggcccgc gccggcaatc
     2041 gcggcaaagt ggtgtccgaa caatacgcct ttgtgcgtcg gaacgtagtc caagccgtcg
     2101 ttgtgccgct cggcgggcgt gagacggttc ggatcgagcc gcatcacgcg gttggcgatg
     2161 tagaggctgt aaaaacggta ggcgatacag taaacggata tggcggcggt aaccatccat
     2221 accgcgctga cctgttcgcc tcggctgagg gccagagtgg taaaggatgc taagccgacc
     2281 agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata aatccttatc
     2341 tcacactgtc ggaatatgtc ggagcggaca ggcaacggtt ggctacccgt aatccgatgc
     2401 cgacacaaac cggagtttga atgattgacg aagtatgaaa cagtgcgctt gtcgggactt
     2461 gtgccttatg cccgcatcaa acagtgcagg aatgtacgcg cacaaacgtt aaacgccgaa
     2521 tagaatttta acgcaaatta gcacactgat agcggttttt acttggaatt tggaaaaatt
     2581 tacattcctc cgggcgggca ggcaggttca gacggcatcg tcaggcaaaa ggcggtgtcg
     2641 gaagacgggt aaagaagggg cgcgcaatcc ggattattga tttatcgtgt ttccttttcg
     2701 gttgaacctc agccctttgg ggcggtaaaa tcagacttta tttgggaggg gcgcaacccc
     2761 ttccgaatca ggacggcaca tagggcggtg ctttaatgtg tcgtcctgtg tgttgaaaca
     2821 tattttcttt ttattgggga gcttttatga atatcaggtt tttcgcgctg accgtatcgg
     2881 ttttgtcttt ggcggcctgt gccgtgccgg aagcgtatga tggcggcgga cgcgggtata
     2941 tgccgcctgt tcaaaaccaa gccggcccgg acgattttcg agcgttttca tgcgagaacg
     3001 gtttgtctgt gcgcgtccgc aatttggacg gcggcaaaat cgcgttgcgg ctggacggca
     3061 ggcgtgccgt cctctcttcc gacgttgccg catccggcga acgctatacc gccgaacacg
     3121 gtttgttcgg aaacggaacc gagtggcacc agaaaggcgg cgaagccttt ttcggcttta
     3181 ccgatgccta cggcaattcg gtcgaaactt cctgccgcgc ccgttaaacg gggcagcctg
     3241 tttgaaccgc cgtccggatt tttcgggcgg ttttttatgc cggttttgtt ttggccgtcg
     3301 gctggttccg gcggttttcg ggcgtggcgg attatttttc gtttcgttct gggtttatcc
     3361 tgtcggcagc ggctcaatgc caactttaaa cctgctccga tttcttcagg gctgttatcc
     3421 aatgataaaa ttacatcgtc tgcatcaatg gcatcccacg cttccggctt gacatggcgg
     3481 ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccccgctcat catgttttta
     3541 aatagggcgc gtctggtttt atagcccaag ttcccgcata ggttggcaac ccaatcctca
     3601 tagcgttgcc gaaatttttc ggtatcaaaa aaatcttggt cttccgaact gtcataaacg
     3661 aaagtcctgc tgtttgccaa cgcttgcaag accgtcgtgc ctaaagtttc attgtcggta
     3721 tccaatggca ggatatgggg ggatataggt ggtctattgc cgttaacccc aaacctgaac
     3781 atgtttgaac aatcagagct tcttcattag cataaaaaac tgcccaataa ttgatcctgt
     3841 ttaaaaatca ttatttaatc tccgtaattt tgactgtaat gttttgactt ttgccatact
     3901 ctaccacgcg ttgtaactgc aatctttgct tcttatatat tgtaccctat caaagggctg
     3961 cattactttt ctttctaaag attactatcg cataaaaagt atttttctta aacgacaggg
     4021 atgcgcccgt gccatattca aacaccgccc ggatgttgcg ctgcccgatc ggatgcttca
     4081 gacggcatcg gaagggtttg cagttttgga atatgaagat gataatgtcc gcgagattga
     4141 cggcatttga aagcggttat cccgtatacc ggaggatacg aaatgaacga atattcccaa
     4201 ttaatcaagc atcccgatat ttccctttcc ccgatttcag acggcatcgg ggtcggcaat
     4261 ccggcgacgg gcgaaatttt ggcgtatgtc cgcaacacgg gttcggacaa gctgaaaaac
     4321 ctgattcaaa aggcggcggc agcgcaaaaa ttatgggcgg cgaaaaccgc cttggaacgc
     4381 gccgacatat tgtggcgttg gtatttttta gttaaagaaa acaaagaagc actggcgcgt
     4441 ctcatgacga tggagcaggg caaaagcctg accgaggcgc gcggcgaaat cgattatgcg
     4501 gcttcgtttg tgcgctggtt tgccgaagag gcgcggcgga ttgacggcga tgtgctgacg
     4561 agtgtgaaag cgtcgcaaaa actggttgtg ttgaaacagc ccgtcggcgt taccgctgcg
     4621 attacgccgt ggaatttccc gtccgcgatg atagtcaaac ctgcatcgct cacgcctttg
     4681 agtgcgtatg cgttggctgt actggcctac gaagcgggcg tgccgcagga tttgcttccc
     4741 gtcgtcagcg gccgcgcttc ggaaatcagc catgaatttg ccacgaaccc gaccgtgcgc
     4801 aaaatcagct tcaccggctc gaccgaagtc ggcgcgaaaa tttttgccgg cagcgcggcg
     4861 gacattaaaa aactcagttt ggaactgggc ggcaacgcgc cgtttatcgt gtttgacgat
     4921 gccgatttgg acaaagccgt cgaaggcgcg ctggcgagca agttccgcaa cagcggtcag
     4981 acctgcgtct gcaccaaccg cgtttacgtc caatcgggca tttacgacgt attttgccgc
     5041 aaattgagtg aaaaggcagc cgcgctcaaa ttgggcgacg gcttggacga aggtgtgaac
     5101 caaggaccgc tgattgagga aaaagcggta gaaaaagtcg aacagcacat cgccgacgca
     5161 ctctccaaag gcgcggtctg tctgaccggc ggcaaacgca gcgcgttggg cggaacgttt
     5221 tttgagccga ctgtcttaag cggcgttacg gcgcaaatgg cggtagctcg cgaggaaacc
     5281 ttcgggcctt tgtgtccggt gttccgtttt gaaaccgaag ccgaagtcat cgaggctgcg
     5341 aacaatacgg aatacggttt ggcagcttac cttttcacct ccgacaccgc ccgtcaatgg
     5401 cgcgtcggcg aagtcttgga atacggcatg gtcggcatca atacgggctt aatcagtaat
     5461 gaagcggcac cgttcggcgg ggtgaaacgc agcggtttgg gacgtgaagg cagcaaatac
     5521 ggtgcggacg aatatctaga attgaaatat ttgtgcattg atgccgggtg acggatgccg
     5581 tctgaatggc agggtttcag acggtgtcgc attttaagca gtctctatct gttgtacaat
     5641 gcgccctgtt tttacggtta tttttgattt gaatgtaaga tatgatggaa aacggaaaaa
     5701 cattcccgag atggcgtttt gccttgaaaa gtgcgggctg gcacctctta atcagcttat
     5761 tggttgcagg gctggcggcc ttgctggttt ttaaggtttg gtatccttat ccttatgccg
     5821 agctgacggg cggtctgtcg ctttatcagc tggtggtggc ggtcgatatc gtatgcggcc
     5881 cgctgctgac tttaattttg gcaagcccga agaaaaagac aaaggcacgc atggtcgatt
     5941 tttccatggt cggcatcatc cagctggcgg ctttggcgta cggtttgcac agcgtttcgc
     6001 tggcgcgtcc cgtggtggaa gcgtttgaac aggaccgcat gaccattgtt acggcggcgg
     6061 aagtcgtggt cgaagatttg cacaaagccc ccgaagggct gcaaagcctg tcgtggttcg
     6121 gcatccgccg cattgcattg aaagaacctg aggatgcgga tgagaagaac aagacgctgg
     6181 atttgtccct gaaaggtatc gagccgagta tgcgtcccga ccggtggctg ccgtattccg
     6241 acaaggaagc agaaaaaatc cgcaaacatc tgaaaccgct gaaagtcttg gcggatgcga
     6301 gaaaaacgac ggttgcggac attctgaaac aggcaggtct cgccgaaggg gaggagctgt
     6361 attacctgcc gtttaccagc agcaggcaga aagagtggat agtcattacc gataaagagg
     6421 gcaacaccaa aggctacgcg ccgatagacg gcttcatcat cccttaacca ttgggattcc
     6481 gcccgcactc gaacatccgt tcttcgcggc ggtagaatca gactgtattt gagaggggcg
     6541 caacccctca atcagggcaa cacataggga gacgctttat gtgccgtccg tgtattgaaa
     6601 catttcaaaa taagcccatg ccgtccgaac atcctttcag acggcatggc gttttgccat
     6661 tgccccgata agctgttaaa ctgtttaaat tatttcgacc gaaggtacac gcacatgaaa
     6721 gaccccgagc agagcagtaa gcccgcccgc cgttttttgt gcgtaccgtc tgttcaggca
     6781 gcttgagcgt ttcaggctgc ctttttgcgt ttgaacttga ggaacacgaa aatcatggat
     6841 ttcagttggt tggcagaacc gcatacctgg ataggttttg ccacgctttt ggtgttggaa
     6901 gtcgtattgg ggatagacaa ccttgtcttt gtggcgattt tggcaaacaa ggtccagccc
     6961 gcacggcgcg accgcgcacg gattatcggg ctggggctgg cagtcgtcat ccgcatcatt
     7021 atgcttgctt ttatggcgca catcatcacg ctgaccgagc cgctgttcca aatcggcggc
     7081 ctcgccgttt ccggcaagga catgattatg ctcgcgggcg gtattttcct gctttacaaa
     7141 gccaccaccg aactgcatga acgcctcgaa gggcacaacc gttttaccgt tgccgacaac
     7201 caaaaaaaac acgcgccgtt ttggggcgtg gtcgcgcaaa tcctgatact ggatgccgtg
     7261 ttttccatcg attcggtcat caccgccgtg gcgatggtcg atcacatcgt cgtggcgatg
     7321 ggcgcggtcg tcgtcgcaat ggttgtcatg atttccgcca gcaaactctt gaccgaattt
     7381 gtggacaggc atcccaccgt cgtgatgctc tgccttggtt ttttgttgat gatcggtttc
     7441 agcctgattg cggaagcctt ccatttccac attcccaaag gctacctcta cgccgccatc
     7501 ggcttctcga ttttaatcga actgtttaac cagatttcgc aacgcaacag ccgcaaaaac
     7561 gactacatcg gcagctcgtg gcgcaagcgc accgccgaaa acgttttggg tatgatgggc
     7621 atacgcgaaa gcgtgcttgc tgacgcgggc ggcgaatccg tggatgacgc gcattttgaa
     7681 gaaaacgaaa aatcgatgat acgcagcgtg ctgacgcttg ccgaacgccc gattatgggg
     7741 gtgatgattc cacgccgcga catcgaacgg ctggacattt cccaaagccg cgaagaacag
     7801 tatgcccaac tgcaaaacac gccttacagc cgcctgctcg ttgtcggaaa ggcgggcgtg
     7861 gacgaaccgt tgggctacat caacaaaaaa gacctgctgt cccaactgct ggaaacaggc
     7921 ggtctcgaca ttcagacggc attgcgccag ccgctcgtcc tgcccgacgg cacgaccgcc
     7981 ttgggcgcgc tcgaactctt ccgccaaagc agcgcggatt acgctttggt ggtggacgag
     8041 ttcggcgcgg tgttgggtat ggtgacgatg aaagacctgt tggaagccat cgcaggcgag
     8101 ttccccgaag aattcgagcg cgaagaagaa cccgccgttc aggggaatcc cgatgaaagc
     8161 ctgacggtgg aaggcgcgtt ggaatatgtg gagctggcat cccaactcaa cctgccgcag
     8221 caggaggaag ataccgattt ccataccgtt gccgggctga ttatggaaga attgcaaacc
     8281 atccccgatg tcggcgattt tgccgatttt cacggctggc ggtttgaagt ggtcgaaaaa
     8341 gaagggcagc gcatcgagcg ggtcaaaatc accaaattgc ccgaagaata agcatccagg
     8401 ataaaaaatg aacgttttga tttccaacga cgacggctat ctggcagaag gcattgccat
     8461 tctggcgcgg gttgcgtcgg aatttgccaa tgtcagggtg gtcgcgcccg agcgggacag
     8521 gagcggggtc agcaattcgc tgacgctcga ccgtcctttg cagctgaaac aggcgcaaaa
     8581 cggcttctgc tacgtcaacg gcacgccgac cgactgcatc catgtcggcc agtttgccct
     8641 gcccgatttt aagcccgatg tcgtgttttc aggtatcaac cgtggcgcaa atatgggcga
     8701 cgatacgctt tattcgggga cggttgcggc ggcgaccgaa gcctatctga tgggtatgcc
     8761 tgctgtggcg ttttctttaa acgatgcttc cggacgctat tgggcaaccg cagaaaaggc
     8821 actgtggaca ttgttggcgc atttttttaa aaaaccgccg tccgcgcccg tcttgtggaa
     8881 tgtcaacatc cctgctgtcg caccggaaga tgtcagggga atcaagatta cccgtttggg
     8941 cagacggcat catgagcaga acatcgtccc gtcccgcaat ccgcgcggcg aacagattta
     9001 ttggataggg ccggtcggcg aagtttccga tcgggaagag gggacggatt ttggtgaatg
     9061 cggcgcaggt tttattaccg taacgccgct gcaaatcgat ttgaccgcct atccggacat
     9121 ggcggagacg gcggcctttt ggcatacgga ctgaccgttt ttcaaatacc gtcgggtttt
     9181 gcgcttgaga agaacgcgca tttttattat gatataccgc attcaggcta tatatcggaa
     9241 catgaaggtt atcttatgtt gaaacaaacg acacttttgg cagcttgtac cgccgttgcc
     9301 gctctgttgg gcggttgcgc cacccaacag cctgctcctg tcattgcagg caattcaggt
     9361 atgcagaccg tatcgtctgc gccggtttac aatccttatg gcgcaacgcc gtacaatgcc
     9421 gctcctgccg ccaacgatgc gccgtatgtg ccgcccgtgc aaactgcgcc ggtttattcg
     9481 cctcctgctt atgttccgcc gtctgcacct gccgtttcgg gtacatatgt tccttcttac
     9541 gcacccgtcg acatcaacgc ggcgacgcat actattgtgc gtggcgacac ggtgtacaac
     9601 atttccaaac gctaccatat ctctcaagac gatttccgtg cgtggaacgg catgaccgac
     9661 aatacgttga gcatcggtca gattgttaaa gtcaaaccgg caggatatgc cgcaccgaaa
     9721 accgcagccg tagaaagcag gcccgccgta ccggctgccg cgcaaacccc tgtgaaaccc
     9781 gccgcgcaac cgcccgttca gtccgcgccg caacctgccg cgcccgctgc ggaaaataaa
     9841 gcggttcccg cccccgcgcc cgccccgcaa tctcctgccg cttcgccttc cggcacgcgt
     9901 tcggtcggcg gcattgtttg gcagcgtccg acccaaggta aagtggttgc cgatttcggc
     9961 ggcggcaaca agggtgtcga tattgccggc aatgccggac aacccgtttt ggcggcggct
    10021 gacggcaaag tggtttatgc cggttcaggt ttgagggaat acggaaactt ggtcatcatc
    10081 cagcacaatt cctctttcct gaccgcgtac gggcacaacc aaaaattgct ggtcggcgaa
    10141 ggtcagcagg tcaaacgcgg tcagcaggtt gctttgatgg gcaataccga tgcttccaga
    10201 acgcagcttc atttcgaggt gcgtcaaaac ggcaaaccgg ttaacccgaa cagctatatc
    10261 gcgttctgac tttccgtttg caatcaggtt aaaaatgccg tctgaaaggt cttcagacgg
    10321 catttttgtt ttggcggctg tgttttcaaa tcattccgaa gggacggggc gtataaattt
    10381 gtttgactgt gtttgggtat ttgcttgatt tcatccgttt gcaatggaaa agccgcttat
    10441 ttccgttcgg agggcggttt tatcggataa aaggcatttt gtccgactga ttagggctgc
    10501 atcaagggaa tatgaaaaca caaccgctta gtctcacggc tgtgttccaa aatggcgttg
    10561 aaaaacggat gagccagaat cagccgattt tacttcaata aatcaaacca ttacaccgaa
    10621 tgtcggaaca atgcttgtcc tgtctttcag acggcatagg ccgccgtcag cagccgcagg
    10681 acacgtctcc ggaggcttgg aggctgattt ctctgcgttt tttggctttt tcgtagcggc
    10741 agcgttggcg gtcggtcagg atttccagcg gagggacggg gacgggtttg ccgtctttga
    10801 ccgcaaccat cgtgaagtag cagctgttgg tatggcggat ttcgccggtg cggatgtttt
    10861 gtgcttcgac acggatgccg atttccatcg aggtgcgtcc cgtgtagttg acagcggcgt
    10921 aaaaggtaac caaatcgccg atgtggatgg gttctttaaa caggactttg tcaaccgaaa
    10981 gggtaacgca ataattgccg ctgtaacggc tggcgcagga ataggcaacc tggtcgagca
    11041 ggagcaggag ttcgccgccg tgtacgttgc cgctgaaatt ggcggtgtcc ggcatcatca
    11101 gttcggacat aatgagttcg tgggaaggca gttggcgttg ttgtgtcatg gtgtacctgt
    11161 tttaagaaag ggtgttttga aatgggtgcg ggaaaatttt agggaatggg gaagtggaat
    11221 tcaagctaaa aaaagctatg gagggatagt ctgccgaaat gggccgccga tgccgtctga
    11281 agccttcaga cggcattttg ttcaggaaag ccaaaaacgg aaaaccaaga aaaacacgag
    11341 tgccagcacg cccgcccaaa cggcgagcat gaatccggca accgcccgtc ccatacggta
    11401 aaccggtttt ttgcgttcgt cttcgatggg ggaggcgtgg tgtttttcgg gttgttcgga
    11461 cattttgttc tcaatcggaa aagttggaaa cgggcgtaag gttacgctgc cgtgcaaaaa
    11521 gatttaagaa ggaaggtcag cagctgtctt cccaattttc gtcccaagcg tgtttcaatg
    11581 ccgtctgaac ggtatcggca tcaaaaccgc gataggcgag gaaacgcgcc tgtttttgtt
    11641 tttctttgag gttggcggcg gggtgtttga attttttacg caacacggct atggcggctt
    11701 gcttttcgct tgagcggtcg ggaagcaggt tgcggctggt tttttcatct atgccctgtt
    11761 gcgccaaagc ctgtttcagc ctcaatgaac cgtgtttgcg gcttttgctg cggatatagg
    11821 cttcggcgta gcgcaaatcc gactgccagt tgcgttcggc aaattcgttt aacacgtttt
    11881 ccaactcctc ttcgctttcg gcgtgcggtg caagtttgcg tttcagaccg atgcggctga
    11941 cttcttggcg cgagaggatg tccatcgcgc gggcgcgtag ggatttttgc ggtttcatgg
    12001 cggcggtttc ggcacggaaa gattcagacg gtatgggaaa cggattccga tgccgtctga
    12061 acagattgtt ttattcttcg tctttgtttt tctgcggtcg caggttttcg taaatttcgg
    12121 gcagcgcgcc gataatccgg tcggggcggg tcgtatcgtc ttgcgagagc agcgtcatat
    12181 cgccgtaacc gaaggtaacg ccgacactca ggcagccggc ggctttggcg gcgatgatgt
    12241 cgttgcgcga gtcgccgacc ataagcatat tggcggcatc gatacccaaa acttcggcgg
    12301 cgtgccgcag cggcagcggg ctgggttttt tttcgggcag gctgtctccg ccgagtatca
    12361 ggctgaagta gtcggcgagt cccagttgtt ttagaagctc ggcggcaagg atttcgtttt
    12421 tgttggtaat gattaccagc ggaatgccca aggatttgag caatgccagc ccggcttcgg
    12481 tttcgggata ggggcgggtg aagacgctca aatggtcgcg gtagtatttc atataggaca
    12541 cgaaaccttt ttcccacagt tcggaatcgg cttcgcggtc gcggtcgttg gtgaggacgc
    12601 ggtgaaccag tttgccgatg ccgtcgccga cgtagctttc gaccactttg gcgggcagcg
    12661 gtttcatacc gagttgttcc aacatcgctt ctgcggcggc ggcaaggtcg gggacggaat
    12721 cgcacagtgt gccgtccaaa tcgaaggcga cggcttggac gtgttcgatg gctgcattca
    12781 taatccggtt tcctgtcggg ttgaaagggt ttattttaac atcgtttgca cagggcttct
    12841 aatgtataga ttattaacaa tcgcaacagg tttttcgggc ggtgttttcg gattttatgg
    12901 cggggtctgg caagggtttg aaatcccggc gatgttccaa ttaaattttt aaaaaataaa
    12961 atgatgacta tatctttatt taattggaca tttgcttttc ggatgcgcgc tgcttttttc
    13021 agacggatgc tgtgcggctt gaaaaataaa atcatatata gattaataaa atcgggctta
    13081 tgtcgactac atgctattac gttttgtcgg gaaattggtt atagtaatga gcgaaatgca
    13141 cgcccctgaa aatctttttg cgaaaaggaa gcaaaatgtc tgatgccgtt gcaaaagaaa
    13201 ccctcaatcc gtttgaaatc gcgcgaaaac aggtcaaaac cgcctgcgac cggctgaaaa
    13261 ccgatccggc cgtttatgaa atcctgaaga gtccgacccg cgtgttggaa gtcaaactcg
    13321 acgacggcac ggtcaaaacc tttaccggtt accgttcgcg tgcgttccca tcccggtgtg
    13381 aatctggacg aagtcaaggc actgtcgatt tggatgacca tcaaatgctg cgttgcaggc
    13441 attccttacg gcggcggcaa aggcggcgtt accttggatc cgcgcgatta ttccgaggcg
    13501 gaactggaac gcatcgcccg agcttatgcc gaagcgattg ccccgctgat cggcgaaaaa
    13561 aatcgatatt cccgcccccg atgtgaacac caacggcaaa attatgtcgt ggatggtgga
    13621 tgcctatgaa aacgtggtga aacattccgc gccgggcgta tttaccggca aaccggttga
    13681 gttcggcggc tctttggcgc gtaccgaggc taccggttac ggcgtgaacc tcgccgccgt
    13741 ccaagctttg gaaaaactgg gcaaagatgt gaaaggcgca acctacgcca ttcaaggttt
    13801 cggcaatgtg ggctaccaca ccggctatta cgcgcatcaa tccggcgcga aagtcgttac
    13861 cgtatctact gttgacgttg ctatctacaa tgaaaacggc ttggatatgg aagcgctgtt
    13921 taaagagttc caagaaaaag gcttcatcac caacaaagcc ggttacggca aagaaattac
    13981 caacgccgaa cttttggctt tggatatgga cgtactcgcc ccttgcgcat tggaaaacca
    14041 gctgacttcc gaaaacgccg gtaaagtccg cgcgaaaatc gtggttgaag gcgcaaacgg
    14101 cccgactacg cccgaagccg acgttatcct gcgtcaaaac ggcgtattgg tcgtgcccga
    14161 tattttggca aactgcggcg gcgtggtcgt ttcctatttc gagtgggtgc aaaacctgca
    14221 aggctattat tgggagtttg acgaagttca ggaaaaagaa atcgtcgtcc tgcgccgcgc
    14281 gttccgcgat atttggaact tggcgcaaga gtatgatgtc gatttgcgta ccgcgtctta
    14341 tatgatgagt atccgccgtg ttgaaaaagc gatgaagctg cgcggctggt attgatgttt
    14401 gaacaaagga tgccgtctga aaccatttca gacggcattt ttgtttggac ggaattaatg
    14461 gtgagaatgg ccgatttgga aggttaaagt tgtgtagttc gcctgttttt ggcacaggct
    14521 ttgatcgggg aaatcggctt tgtattcgac actcgctttc caaaagcctt ggcgcaaggg
    14581 gatgatgtcc acttcgcctt tgccgtcggt ggtgtcggag aaggcttggg cttcggtttt
    14641 gtgcgttttg ctgcggtcgc tggtgtcgaa gccgtcaaat gtagcggtaa cggtggcatt
    14701 gggcagcggt tcgccacgga acagaacgcg gactttgaag cgttcgccta cgtgaatgtt
    14761 ggcgggattg tccagcggga cgatttccaa gttttgtccg accggtttgg tgatgatggc
    14821 ggtgtccgcg ctttcgtgtc ccacgttgac aatgttttta ccgaacatac gggtttgttc
    14881 gcaatagctt gcgtcaggca tttctttgat gccagcctgt ttccagcctg ctttgttttt
    14941 tgaccggaaa gtaggctgat attcggcggt aacgaggtag ctgccgtctt tgacgggacg
    15001 gttgctgcgg tattggtagt tgtatgtgcc gcgttgaatc atgttttcct tacctttttc
    15061 ggtaaccagc tgcatcggtt tgctgaagat gtgcaggcgg tctttggcga tgggttcgag
    15121 ttcggggaat tcgccgtagc ccaagtcggc tttaaggtat tcgccgccgt gcgtgtgggc
    15181 ggtttcgacc cagacgcggt gggcgtgtgc ggatgtggca aatagtgcgg aaacggcgag
    15241 cagtgtcaat gttttcttca tggtgcagcc tttatatcgt gcgggttaaa aattttgtta
    15301 tgctataaca aaatatgtat cgtgtgaaga cggatttgcc gcctgaaaac tgtctttttg
    15361 tatcgatttg aatttttcag aaaatgaagc ggacgtttgg gcggaggcaa cttgtttgat
    15421 aagatagcaa tatttcaaaa cggagaaata tcatgcctta tgtcaatatt aaagtaaccg
    15481 gcggcaagga agcaccgact gccgcgcaaa aagcggaact gatcggcggc gtaatcgaat
    15541 tgctggcacg cgtgctgggt aaaaatcccg aaacaacggt tgtcgtgatt gacgaagtgg
    15601 ataccgataa ctgggatata ggcggcaaaa gcgtcagcga acggcgcaaa gagggcaggt
    15661 aaaaagcctg aaaaccccgc cctgatgctt taaatttcgc gtgaaaaaga gtacattccc
    15721 acccattgcc caaaatttac ggaacacatt atggataaat ttcccaagtc tgcaaagctc
    15781 gaccacgtct gctacgacat acgcgggccg gttcacaaaa aagcccttca gttggaagag
    15841 gagggcaaca aaatcctcaa gctcaatatc ggcaaccctg cgccgttcgg ctttgaagca
    15901 cctgatgaaa tcttagtcga tgtcatccgc aacctgccga cttcgcaagg ctattgcgat
    15961 tcgaaggggt tgtattccgc acgcaaagcc attgttcact actatcagaa caaaggtttg
    16021 cgcgatatta cggtaaacga tgtctatatc ggcaacggcg tgtccgagct gattgcgatg
    16081 tctatgcagg cgttgctcaa cgacggcgac gaaatcctga ttcccgcgcc cgactaccct
    16141 ttgtggacgg cggcggcaac gcttgcgggc ggtacggtac gccattatct gtgcgacgaa
    16201 gaaaacggct ggtttcccaa ccttgccgat atggaagcca aaatcacgtc caaaaccaaa
    16261 gccatcgtcg tcatcaaccc caataacccg acaggagcgg tgtacagcaa ggaaatcctg
    16321 ctggaaattg ccgaactggc gcgcaagcat ggtttgatta ttttcgccga cgagatttac
    16381 gacaaaatcc tttatgatgg cgcggttcac tgccacatcg ccgcgctcgc ccccgacctt
    16441 ttgacggtaa cgttcaacgg tttgtccaaa gcctaccgtg tagccgggtt ccgccaaggc
    16501 tggatggtgc taaacgggcc gaaacatcac gcaaaaggtt atatcgaggg tttggatatg
    16561 ctctcgtcca tgcgcttgtg tgccaatacg ccgatgcagc acgcgattca gacggcattg
    16621 ggcggttatc agagcattaa cgaattcatc ttgcccggcg gacggctttt ggaacagcgc
    16681 aacagagcgt gggaactggt caaccagatt cccggcgtgt cctgcgtcaa accgatgggc
    16741 gcgatgtata tgttcccgaa aatcgatacc gaaatgtacc gcatccgcga tgatatgaaa
    16801 ttcgtttacg atttgctggt gcgcgaaaaa gtcctgctgg tacagggaac ggggtttaat
    16861 tggatcaaac ccgaccattt ccgcattgtt acgctgcctt acgtccatca gattgaagag
    16921 gcgatgggca ggttggcaag attcctgcaa acctaccatc agtaggattc tgataaaaaa
    16981 tgccgtctgg aacggagatt cccgtttcag acggcatttt caacagcagg aacaaatcag
    17041 gcaaatttca gtctgtcgcc gtcggcttct accttgattt cgctttcggg cgcatagttt
    17101 ccggcaagca gggctttggc cagcgggttt tcgatttccg attggatcgc gcgtttgagc
    17161 ggacgtgcgc cgtaaatcgg gtcgaaaccg gctttggcga tgatgtccag agcggcatcg
    17221 gaaacagtca ggcgcaggtt ttgtttttcc aaacgttttt ccaaaccttt gagctggatt
    17281 ttcgcaatac tgcggatatt ggcctgatcc aagccatgga acacgaccac ttcgtcgatg
    17341 cggttgatca tttccggacg gaaatgttct ttcacatcct ccatcacaac ttctttcacc
    17401 gcttcgtagt cctgcgtgcc catttgttgg atgtgctggc tgccgatgtt ggaagtcatc
    17461 acaatcacgg tatttttaaa atctaccgtc ctgccttgtc cgtcggtcaa acgaccgtca
    17521 tccaatactt gcagcaggat gttgaatacg tcgggatggg ctttttccac ttcgtccaaa
    17581 agaatcacgc tgtacggttt gcggcggact tgttcggtca ggtagccgcc ctcttcgtat
    17641 ccgacataac ccggaggcgc gccgattaag cgggcaacgg cgtgtttttc catgtattcg
    17701 gacatgtcga tgcggatgag gtggtcttcg ctgtcgaaca ggaagcctgc caaagctttg
    17761 cacaactcgg tcttacccac accggtcggg cctaagaaca ggaagctgcc gtaaggtttg
    17821 ttcggatcgg caaggccgga gcggctgcgg cggatggcat cggacacggc gcgcacggct
    17881 tcgttttgac cgactacgcg gcggtgcaac acttcttcca ttttcaacaa tttatcgcgt
    17941 tcgccttcca tcattttgga tacgggaatg ccggtcatac gggaaacgat ttcggccact
    18001 tcatccgcgc cgacattatt acgcaagagt ttgtttgccg gttttgtgct gtccgtatct
    18061 gcccgttcgg cggctgcacg ctgtttttcc aaatgctcca aatcttcata catcaatttt
    18121 gaagccagtg ccaaatcgcc ttgccgtttt gcctgttcga ttttaatttt gacttcgtca
    18181 atttgtttct taatattggc agcaccgtct gaaatcgctt tttcggcttt ccagatttcg
    18241 tccaaatcgg cgtattcttt ttgcaagccg tcgatttcct cgtctatcag ttccaaacgt
    18301 tttttgctgg catcgtcttt ttctttttca acgtgcgcct tttccatccg aagctgaatc
    18361 aaacggcggt cgattttgtc catagcttcc ggtttggttt ctttttccat cttgacacgg
    18421 ctggcagctt cgtcaatcaa atcaatcgcc ttgtcgggca aaaagcggtc ggtaatgtag
    18481 cggtcgctca actccgctgc ggcaacaatg gcggggtcgg taatatcgat gccgtggtgg
    18541 atttcatagc gttcctgcaa accgcgcagg atagcgatgg tgtcttccac gctgggttcg
    18601 ccgaccaata ctttttggaa acggcgttcg agtgccgcgt ctttttcgat gtattggcgg
    18661 tattcgtcca aagtagtcgc accgatacag tgcagttcgc cccgcgccaa ggccggtttc
    18721 aacatattgc ccgcgtccat cgcgccgtcg gttttgcccg cgccgaccaa agtatggatt
    18781 tcatcgatga aaatcagggt gttgccgtcg tctttcgcca aatcgttcaa tacgcctttc
    18841 aagcgttctt caaattcgcc acggtatttc gcaccggcaa tcaaagccgc caaatccaaa
    18901 accagcaggc gtttgttacg cagggattca ggtacttcgc cgttgacgat gcgttgcgcc
    18961 aaaccttcga cgatggcggt tttacccaca ccgggttcgc caatcagcac ggggttgttt
    19021 ttggtgcggc gttgcagcac ctgaatcgcg cggcggattt catcatcacg accgataacg
    19081 gggtcgagct taccgtctcg ggcgcgttgg gtcagatcga gcgtgtattt tttcaaagca
    19141 tcgcgttggt cttcggcatt cgcatcgttc acgttttgtc cgcctcgtac cgcatcgatc
    19201 gcggcattga tgttttgttc ggtcgcaccg gcttctttca agattttgcc ggccgcatcg
    19261 ttctgctgca ccaaggcgag caggaaaagt tcgctggcga tataggcatc gccgcgtttg
    19321 gttgccgctt tgtccatcag gttcaacacc gcctgcaatt cgcggctggg cagaatctca
    19381 ccaccttgtc cggacacttt cggcaggctg ttcaaatgct gctgcaaacg ctgtttaacc
    19441 tgcggcacgt tcacgcccgc atgagccaag agcgcggcag ctccgctgtt ttggtcgtca
    19501 agcagggctt tcagtacgaa acctgcttcc agatagctgc tgtccgcagc caacgccaaa
    19561 ctctgagctt ctgcaagggc ttgttggaat ttggcggtta atttgtcgta tcgcattttc
    19621 gtttcctttt caaaatgtcc gctgtcgaag cctatatgtg catgactgtg gataactcaa
    19681 gtgttgtttt ctgtttttct atatttaatt cgatatattg ttgaatttaa agtatataga
    19741 aatgtatgat aatatgtata actatatctt cttaatatgg aaaagtctgt tgtcggctgg
    19801 atgtatgtgg caaatcgggt ataatcggcg catcttttcc ctttcagacg gcattgctgc
    19861 cgtaaggaca tttttatgag caaaaaacga gttctgaccg gcgtaaccac caccggcacc
    19921 ccgcatctgg gcaactacgt cggcgccatc cgccccgccg tccgcgcggc gcaaaacccc
    19981 gataccgaat ccttcctctt cctcgccgat taccacggca tcatcaaatg ccacgagcag
    20041 gagatgattc accaatccac ccaagccgtt gccgccactt ggcttgcctg cggactcgac
    20101 cccgagcgca ccaccttcta ccgccaaagc gacattcccg aagtgatgga gttgaactgg
    20161 attctgacct gcatcaccgc caagggtttg atgaaccgcg cccacgccta caaagccgcc
    20221 gttcaagcca atgcggaaaa cgggcaggaa gaccccgatt tcggtgtgga aatgggtttg
    20281 ttcagttatc cgattctgat gactgccgac attttgatgt tcaacgccaa cgaagtcccc
    20341 gtcgggcgcg accaaatcca acacgtcgaa atggcgcgcg acattgccgg ccgcttcaac
    20401 caccgtttcc aagaactctt caccctgccc gaagtgaaaa tcgacgaaaa cgtcgaactc
    20461 ttggtcggtt tggacggacg caaaatgtcc aaatcctacg gcaataccat tccgctttgg
    20521 gaaaacgaca aaaaaaccca aaaatcggtc aacaaaatca ttaccaacat gaaagagccg
    20581 ggcgagccga aacagcccga cgaaagccct ttgtttgaaa tctataaagc cttctccacg
    20641 ccgtctgaaa cggcggaatt tacgcaaatg cttgccgacg gcttggcttg gggcgaggcg
    20701 aaaaaacttt cggcggcgaa aatcaacgcc gaactggcag aactgcgcga acgctacaac
    20761 gcattgacct ctaatccttc gcaaatcgaa gagattttgc aggcaggcgc gcaaaaagcg
    20821 cgcaaagaag cgcgcgaatt gttggataaa gtacgcgatg cggtcggcat ccgcccgttg
    20881 aaataaaccc aatgccgtcc gaacctccgc ctgccacgtt tcagacggca tttcagacgg
    20941 cattttgaaa ccatcaggag tgcggaatgc tgaaaatacc ttttgccgtg ttgggcggct
    21001 gcctgctgct tgccgcctgc ggcaaatccg aaaatacggc ggaacagccg caaaatgcgg
    21061 cacaaagcgc gccgaaaccg gttttcaaag tcaaatacat cgacaatacg gcgattgccg
    21121 gtttggcttt gggacaaagt agcgaaggca aaaccaacga cggcaaaaaa caaatcagtt
    21181 atccgattaa aggcttgccg gaacaaaacg ccgtccggct gaccggaaag catcccaacg
    21241 acttggaagc cgtcgtcggc aaatgtatgg aaaccgacgg aaaggacgcg ccttcgggct
    21301 gggcggaaaa cggcgtgtgc cataccttgt ttgccaaact ggtgggcaat atcgccgaag
    21361 acggcggcaa actgacggat tacctgattt cgcattccgc cctgcaaccc tatcaggcag
    21421 gcaaaagcgg ctatgccgcc gtgcagaacg gacgctatgt gctggaaatc gacagcgagg
    21481 gggcgtttta tttccgccgc cgccattatt gagttattcg gacatcacgg aatatatttg
    21541 gatttttcaa accttgcgga aaaagtccgc gccgccggga aactcaaaag gaggggatgt
    21601 gtgttacaat tttccgaact gttttcataa aatagttttc ggacgtgttt caatatggca
    21661 ttgatgccgc cttgttgttc gagaaaaaaa cctttatatt taatataatg ggttttaact
    21721 aaacgggaaa ccgttttctc tccggccgat gggcaaaatc agccgattga tggaacacgg
    21781 tccggttttt aagcaaaacc ttaatcaaca actcaagaag gataagacat gaaaaaatta
    21841 ttgattgccg caatgatggc ggctgccttg gcagcttgtt cgcaagaagc caaacaggag
    21901 gttaaagaag cggcccaagc cgttgagtcc gatgttaaag acactgcggc ttctgccgcc
    21961 gagtctgccg cttctgccgt cgaagaagcg aaaggccaag tcaaagatgc tgcggctgat
    22021 gcaaaggcaa gtgccgagga agctgtaact gaagccaaag acgcggcagc cgaaaccaaa
    22081 gaagcggtaa gcgaagcggc taaagacact ttgaacaaag ctgccgacgc ggctcaggaa
    22141 gcggcagaca aaatgaaaga cgccgccaaa taatttgttg ccttggcaaa aatgacgaga
    22201 tgccgcctgc cggcaaccca aatgaaaccg cctgaagatt ttcaggcggt ttttgagttg
    22261 tatgcccctt gttttttacg cctgcatgac cgtgcaggga ggatgtgccg cgcatatcgg
    22321 gatattgcgg acagggaaat gccgtctgaa catcatttag acggcatttg gtttgtcaag
    22381 gttcagacgg cttcaacttt gaacggcata ttgatttttt gccattggac gctttcatat
    22441 tccaatgcgt cggcaatcag ggggtggcgt tccagccatt tcctgtcaat acgcaggatg
    22501 aagccgcagc tttccgtatc cgtacgcaac tgcatatttt tcgggaaacc cagatcttgg
    22561 cgcgaacggc agaacagggc ggcaaggcgc agggacaaaa cggcatacca caacatttcg
    22621 ttgccgccga taatgccgcc catttttttc atatcgccgc gatgaccgat gaccagttgg
    22681 gcaagtatgg tctgttcctt gcgtgaaaaa cccggcatat cggcgttttc gaggatgtag
    22741 gcggaatgct tgtgatagcc ggtgtgggcg atgtccaaac cgatttcatg cagcgcggcg
    22801 gcgcgtccga gatactgttg ccacaaggca agttcttgaa ccgtaacgtt tttggcatga
    22861 cagaggctgt ccataaaggt ttgcgcggtc tcggcggtgc gtttcgcctg attgaggctg
    22921 acgtggtagc ggtgttggaa ctcggcaacc gtttgtccgc gcatatcttc gtttaagccg
    22981 cgcccgatca aatcgtaaaa cacgccgtcg cgcagggcgg cttcggttac ggtcatcctg
    23041 tcgagtttca tttcctcaaa cgccgccatc atcacggcaa gtccgccggc aaaaacttcg
    23101 atgcgttccg gtttcaggtt ttcaaatttg gcttttttga ccgaaccggc ttcgatgatg
    23161 cgttcggcga gggcgcgcat tcccttgtag gtaatgtccg cctcttgggg catttcggcg
    23221 gcaagcacgt cgcggatgga ttttgccgaa cccgatgtgc cgacggcgaa atcccaacct
    23281 tcgcgcctca tatttttgct gatgcgctgg atttcgttgc gggcggcgga aatggcggct
    23341 tggaagtctt tggcggtgat tttgttttgg aagaagcgca ggctgtaggt tacgcagccc
    23401 aagggcaggc tttcggtaat gtcgggattc agcgtcgaac cgatgacaaa ttctgtcgaa
    23461 ctgccgccga tgtcgataac cagcattttg cccccgcagg gggggagggt gtggatcacg
    23521 ccggtataaa tcagccgcgc ctcttcgcgc ccggcgatga tttcgatggg gaaacccagt
    23581 gccgcttcgg ccttgggaag gaaatctgcg atgtttttgg cgacgcggaa tgtattggtt
    23641 gccacggcgc gcacctgctc ggggcggaag ccgcgcaggc gttcgccgaa ttttgccagg
    23701 cagtccaaag cctgttcctg cgaggatgtg ctgaggtttt tctgttcgtc cagaccggcg
    23761 gcaaaacgga ccatctgttt gaaggaatcg atgactttta attgtccgtt gttgttttcg
    23821 caaatctgga ggcggaaact gttggaaccc aaatcgacgg aggcgaggac gtttgcggga
    23881 ttggtggtca tggcggatac cggtggggga aaaacgcaat gttactctga cggcgcaggc
    23941 gttgacaata aatgatgcgg ctgtttttga ttctgctcac ggatgttgcc gacggtatgt
    24001 ttccgacagc aaaaaatgcc gtctgaaaaa cgttcggacg gcattttttg cgcttatttg
    24061 aaatcctttt ccacgctcat gaaaatctgc gtatttttgc gtgtgtaaaa acttttcata
    24121 ttgctgtcga ttttcagata gcggaaattg agttgcggcg taaagccctt ccaagagatt
    24181 ttgtcgtgcc gcaacgacag gtttgcctga tattcgtggt ctttgcgcgg gaagcggtac
    24241 acaattgtcc cgggtgcatc aaaagtcctg cgggtatagc gcaggtttgc ccgcagcccc
    24301 aagccgccgt cgaacgtttt gaccgcgccg acacgcaaac ccttgcggac ggaagcctgt
    24361 tccgcctctt tcgtcatgtc gtgcggccag tccgcaccgc cgtaaagcag ccggtctttc
    24421 ggcgcggaat acatcagcgt cgcgcccgcc agcggcatat ggctgccgta tcgggcggcg
    24481 gtgcggtctt cctgataatg cttccacata ttgcccgcgt tcagcgtcag ccgccagcgt
    24541 tcgcccaagc gtcgggagaa atcggcattg aagccgccgg cgaaattgta tcggctgccg
    24601 cccaagaggt tttgcccgac aaacggcacg gtgccgaacg agcgcgttac cgaacggttt
    24661 tgatggccga acgacaggcg caggctctgt tcgctgaaat ctttgttatc ccaataatgc
    24721 acgccgcggc tgatgccgcc gtagaggaaa tgatgcccgc ccgcattgat ttcgcgcgac
    24781 acgcccaagc cgtagcgcaa accgtgtgcc ttttgcggca ggctgtcggc ggttttcgtc
    24841 cagcttctgc ccgcaaattc aatcgttttt tcggacgaag cgtt
//
LOCUS       DAXDSD010000020        24270 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXDSD010000020 DAXDSD010000000
VERSION     DAXDSD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24270
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.020"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..120
                     /locus_tag="ACFB2Q_000636"
     CDS             <1..120
                     /locus_tag="ACFB2Q_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HFC0917714.1"
                     /translation="ILAVVPKKAEAISNKLFFTHHPKRQTEFAVGQIRQGRAE"
     gene            248..1543
                     /gene="hisS"
                     /locus_tag="ACFB2Q_000637"
     CDS             248..1543
                     /gene="hisS"
                     /locus_tag="ACFB2Q_000637"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC0917715.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            1544..2173
                     /locus_tag="ACFB2Q_000638"
     CDS             1544..2173
                     /locus_tag="ACFB2Q_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC0917716.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            2323..3780
                     /gene="der"
                     /locus_tag="ACFB2Q_000639"
     CDS             2323..3780
                     /gene="der"
                     /locus_tag="ACFB2Q_000639"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC0917717.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFESIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            3811..4710
                     /locus_tag="ACFB2Q_000640"
     CDS             3811..4710
                     /locus_tag="ACFB2Q_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC0917718.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            4762..5179
                     /locus_tag="ACFB2Q_000641"
                     /pseudo
     CDS             4762..5179
                     /locus_tag="ACFB2Q_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5246..5812
                     /gene="dcd"
                     /locus_tag="ACFB2Q_000642"
     CDS             5246..5812
                     /gene="dcd"
                     /locus_tag="ACFB2Q_000642"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC0917719.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(5878..6582)
                     /locus_tag="ACFB2Q_000643"
     CDS             complement(5878..6582)
                     /locus_tag="ACFB2Q_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC0917720.1"
                     /translation="MLRSVLAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGTVQF"
     gene            6726..7921
                     /locus_tag="ACFB2Q_000644"
                     /pseudo
     CDS             6726..7921
                     /locus_tag="ACFB2Q_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            7947..8900
                     /locus_tag="ACFB2Q_000645"
     CDS             7947..8900
                     /locus_tag="ACFB2Q_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC0917721.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTEDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEITPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(9005..9328)
                     /locus_tag="ACFB2Q_000646"
     CDS             complement(9005..9328)
                     /locus_tag="ACFB2Q_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917722.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(9501..10859)
                     /locus_tag="ACFB2Q_000647"
     CDS             complement(9501..10859)
                     /locus_tag="ACFB2Q_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC0917723.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(11217..12917)
                     /gene="recJ"
                     /locus_tag="ACFB2Q_000648"
     CDS             complement(11217..12917)
                     /gene="recJ"
                     /locus_tag="ACFB2Q_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC0917724.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(12986..13699)
                     /locus_tag="ACFB2Q_000649"
     CDS             complement(12986..13699)
                     /locus_tag="ACFB2Q_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917725.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     AAVLVLPMALIAAANSSDK"
     gene            complement(13746..14252)
                     /gene="yjgA"
                     /locus_tag="ACFB2Q_000650"
     CDS             complement(13746..14252)
                     /gene="yjgA"
                     /locus_tag="ACFB2Q_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC0917726.1"
                     /translation="MFEQEDEWISKTQMKKQMNGLQDLGMELTRLSNDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RARVRLLADEGALTQFMSDFPNAGAGRLRTLVRNTKKEQEQDKPPKNFRALFQELKTV
                     MENGGAEI"
     gene            14406..15737
                     /gene="pmbA"
                     /locus_tag="ACFB2Q_000651"
     CDS             14406..15737
                     /gene="pmbA"
                     /locus_tag="ACFB2Q_000651"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC0917727.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(16036..16239)
                     /locus_tag="ACFB2Q_000652"
     CDS             complement(16036..16239)
                     /locus_tag="ACFB2Q_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC0917728.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            16484..16786
                     /gene="clpS"
                     /locus_tag="ACFB2Q_000653"
     CDS             16484..16786
                     /gene="clpS"
                     /locus_tag="ACFB2Q_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC0917729.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            16788..19079
                     /gene="clpA"
                     /locus_tag="ACFB2Q_000654"
     CDS             16788..19079
                     /gene="clpA"
                     /locus_tag="ACFB2Q_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC0917730.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLGLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLNNMVYGQEDAIDALVSAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTE
                     VAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(19119..22217)
                     /locus_tag="ACFB2Q_000655"
     CDS             complement(19119..22217)
                     /locus_tag="ACFB2Q_000655"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC0917731.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVVVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDGNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILSRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKTATIFSFAANEEQNAVGEIVDENFEPEAMDSSAKEFLQAVINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
     gene            complement(22448..23682)
                     /locus_tag="ACFB2Q_000656"
                     /pseudo
     CDS             complement(22448..23682)
                     /locus_tag="ACFB2Q_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359898.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(23849..>24270)
                     /gene="dinD"
                     /locus_tag="ACFB2Q_000657"
     CDS             complement(23849..>24270)
                     /gene="dinD"
                     /locus_tag="ACFB2Q_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFC0917732.1"
                     /translation="TDLAAAAKDAGVEKPVEYAVFQNHGYRGLYGGLDKQGIHSRKGL
                     KKSQRILDHMNASEPAANLFRATQTEEKLRRKNIQGKTQANRVHFEVGQKVRQTIEEL
                     GGIMPENQPVPEKSIKQLENEEQKRLAATEQHQNGKK"
BASE COUNT         5760 a         6791 c         6016 g         5703 t
ORIGIN      
        1 attttggcgg tcgtgccgaa aaaagcggag gcgatttcaa ataaattgtt tttcacacat
       61 catcccaagc ggcagacgga gtttgcagtc ggacaaatca ggcaagggcg cgcagagtaa
      121 gtcaaatgcc gtctgaaaac tgtttcagac ggcattttta cccgtccgca aaccatctcc
      181 atcctcacaa gcccttaaaa atccgctata atcgcccgca atttgatttc agcaccccct
      241 ttttgaaatg gcacaaaaaa tccaatccgt caaaggcatg aacgaccttc tgcctgtcgg
      301 gcaaaaagat ttcaaactca cggctgtgtt ttggcaggcg tttgaggata cggtaaaccg
      361 ttggacgcga gcttacggtt atcagcaaat ccgtacgccg attgtcgagc aaaccggttt
      421 gtttgtccgc tccatcggcg aggaaaccga tgtagtcggc aaggaaatgt acaccttctc
      481 cgattcaaac gattctttga gtttgagcct gcgtcccgaa ggtacggcct cttgtctgcg
      541 tgcggtggtc gaacacaacc ttctgtacaa cagcccgcaa aagctgtggt atatggggcc
      601 gatgttccgc cgcgagcgtc cgcaaaaagg acgctaccgc caatttcatc aggtcggtat
      661 cgaggctttg ggttttgaag ggccggatat tgatgcggaa atcatcgcaa tgtctgccga
      721 cttgtgggaa aaattgggta ttcgcgaata cctgactttg gaaatcaaca gcttgggcaa
      781 ccgtgaggaa cgcgcggcac accgtgcggc attggttgaa tatctgaccc gctatgaagc
      841 gcaactggac gaagacagca aacgccgtct gaaaaccaat cctttgcgcg ttttggatac
      901 gaaaaaccct gatttgcagg aaatctgcaa tgccgcgccg cgtttggtag attacttggg
      961 cgaggcttcg caaaaccact atgcacgctt caaggcgatg ttggacggtt tgggtattca
     1021 atatattgaa aattcgcgct tggttcgcgg tttggattat tacaatcaga cggtttttga
     1081 gtggacgacc gacaaactcg gcgcacaggc gactgtgtgc ggcggcggac gttacgacgg
     1141 tttgattgaa gaactcggcg gcaagcctgc gccgtccatc ggctttgcga tgggcatcga
     1201 acggctgctg cttttggtga gcgaatacgg ttcgctggaa gtgaacgccg cgcctgatgt
     1261 ctatgcaatg caccaaggcg aaggggcgga cttgcaggtg atgaaatacg cacaagcctt
     1321 gcgcgcgcaa ggtttcaatg taatgcagca ttccggctat caaagcctga aggcgcaaat
     1381 gaaaaaagcc gacaacagcg gcgcgcgctt tgccctgatt gtcgcgcaag acgaactggc
     1441 ggacggtacc gttacgctca aagacatgaa cggcgcacac ggtcagcaaa ccgtttccgc
     1501 caccgatttg acagatactt tacaacaatg gaagaacgca taaatggcag cccatctcga
     1561 agaacaacaa gagttagaca actttaaata tttttggaaa accacgggca aatggctgtt
     1621 tgccctgctg attttggcgg cactcggcta cttgggatac acggtttacc aaaaccgtgc
     1681 ggcttcccaa aatcaggaag cggcggcggt gctggcaaac atcgtggaaa aggcgcaaaa
     1741 caaagccccg caaagcgaaa tcaatgccga actgtccaag ctccaacaaa gctaccccca
     1801 ttccatttcc gccgcccaag ccacgctgat ggcggcggca accgaatttg acgcgcagcg
     1861 ttacgatgtt gccgaaggtc atttgaaatg ggtgttgtcc aaccaaaaag acagcctgat
     1921 tcaggcgttg gcggcgcagc gtctgggcgt tgtgttgttg caacaaaaaa aatacgatgc
     1981 cgcgcttgcc gcactcgaca cgccggttga ggcggacttc gcccccctgc tgatggaaac
     2041 taaaggcgat gtttatgccg cacaggaaaa aagccaggaa gccttaaaaa actacggaca
     2101 ggctttggaa aaaatgcctc aagattctgt cggtcgcgaa ttgcttcaaa tgaaactcga
     2161 ttcgctgaaa taaataccgt ctgaaatgcc gctctctctt cagacggcat ttcagacaac
     2221 cccatcctac ggcaattccc gtaaaaccat ccgccgtgtc ccaacggaca cctgctcgtg
     2281 ttcagttcta ccgaacggca cttgacacag aaagacccaa tcatgaaacc aaccatcgcg
     2341 cttatcggcc gccccaacgt cggcaaatct accttgttca accgtttgac gcgcaccaaa
     2401 gacgcgctcg tgcatgacct gccgggtctg acccgcgacc gccattacgg acacggcaaa
     2461 gtcggcagca aaccttattt tgtcatcgat accggcggtt tcgagccggt tgtggacagc
     2521 ggcattttgc acgaaatggc aaaacaaacc ttgcaggctg tcgatgaagc cgatgcggtt
     2581 gtgtttttgg tggacggccg taccggttta acaccgcaag acaagattat tgccgaccgt
     2641 ttgcgccaaa gtccgcgtcc cgtttatttg gccgtgaata aaggcgaggg cggcgacagg
     2701 gccgtacttg ccgccgagtt ttacgagctg gcattgggcg agccgcacgt tatttccggc
     2761 gcgcacggcg acggcgtgta ttacctgatt gaagaaattt tagaaaactt ccccgagcct
     2821 gaagccgaag aagccgatgc aaaacatccc gtttttgccg ttatcggtcg tccgaacgtc
     2881 ggcaaatcta cgctggttaa cgccattctc ggcgaaaaac gcgtcatcgc cttcgatatg
     2941 gcaggcacaa cgcgcgacag catccacatc gatttcgagc gcgaaggcaa accgtttacc
     3001 atcatcgata ccgcaggcgt gcgccgtcgc ggcaaagtgg atgaagcggt ggaaaagttc
     3061 tccgttatca aagctatgca ggcggttgaa gcggcaaacg ttgctgtttt ggtattggac
     3121 gcgcaacagg acattgccga ccaagatgcg acgattgccg gttttgcttt ggaagcggga
     3181 cgcgcgctgg tagttgccgt caacaaatgg gacggcatca gcgaagagcg gcgcgaacaa
     3241 gtgaaacgcg acatttcccg caaactgtat ttcctcgatt ttgccaagtt ccactttatt
     3301 tccgcgttga aagagcgcgg tatagacggt ttgtttgaaa gcattcaagc tgcctacaac
     3361 gcggcgatga ttaagatgcc gacgccgaaa atcacgcgcg tattgcaaac cgccgtcgga
     3421 cgccaacagc cgccgcgtgc cggcttggtg cgtccgaaaa tgcgttatgc ccaccaaggc
     3481 ggcatgaatc cgccggtgat tgtggtacac ggcaattcgc tgcacgcgat ttccgacagc
     3541 tatacgcgct atctgaccca gaccttccgc aaagccttca acctgcaagg cacgccgttg
     3601 cggattcaat acaatgtttc ggaaaacccg tatgaaaatg cggaagacaa accgaagaaa
     3661 aaaccgctgc gccgcgtcag cctgagcaac cgtattgaga aacgcgaagg ccgaaaggaa
     3721 gagaaaaacc gcttcaagaa gaaaaccaaa gtcagtgtga aaaaacaatt cagcaaataa
     3781 ttcccgacat ttcaaacaat aggaaacatt atgtggttca agcagattag tttttatccg
     3841 ctcaacaaag aaaagctgcc tgaggcggac gtacttgccg ataaacttgc tgaagctgaa
     3901 tttacccatt gccaaggttt ggactggttc agcgaaggct ttaccgcacc ggtttctttt
     3961 tcgcccgaac tcgttttccc tgccgacttt accttgcgcg tcgccctgaa aaaagaggaa
     4021 aaagtcctgc ctgccggcgt catccgcgat attttggaag agaaggtagc ggaaatccaa
     4081 aacaatgaag cccgcaatgt cggtcgcaaa gaaaaacaag agcttaaaga gcaaattaca
     4141 gacgacctgc tgccccgagc gtttacccgc agcagccgta cagaagcggt gtttaacacc
     4201 cgccacggct acctgctcgt caataacgcg gcttccgcca aagcggaaaa catcctgacc
     4261 aagctgcgcg aagctttggg aggtttggaa gcctcgctgc cgaataccaa acaatcgccc
     4321 tcttccctga tgaccggctg gctgttgcaa gggcattgcg aaggcggttt tgaattagac
     4381 agcgattgcg aactcaaagg tacgggcgat attgttcccg tcgtcaaagt atccaaacaa
     4441 gacttaaccg ccgacgaagt ggttcaacac gtcaaaaacg gtaaaaccgt tacccggctc
     4501 ggtttggttt ggcgcgaaca aatcgccttt atcctcactc aagacttcac actcaagcgc
     4561 atccaatacc tcgacgtatt gcaggaagaa gccgaaagca acggcgacga tgccgccggc
     4621 cttgccttcg cctcgcaaat cctgatggcg gaatccgtca gcaccatgtt ggaagagctg
     4681 gtttcctatt tgggcggctg gcaagattga tttttcagac ggcttttata ccgtcagcca
     4741 aaatccaatg gaataaccct tatgcttaaa catctcgcat tcctactgcc cgccatgatg
     4801 ttcgccctcc ccgcccagac cgccgtccta agcccctatc aggaaaccgg ctgcacctac
     4861 gaaggcggga tcggaaaaga cgggcttcct tcaggcaaag gcatatggcg ttgccgggat
     4921 gggcgcggtt ataccggttc attcaaaaac ggcaaattcg acgggcaagg cgtttatacc
     4981 gttgccgccg gccgcgaagt atttctcgag ccgttcaatt ccgacagtac caaattccgc
     5041 aatatggcat tgtcgggcac gttcaaacaa ggcttggcac acggcaggtt cgccgcctcg
     5101 caaaacggcg aaaccctctt ttattatgaa atgcgaacac ggcatgatta aagaagtgaa
     5161 actgcccaaa aacaaataac cccccgtttt tcaaacagca tcccccaatg ctttatccga
     5221 acaccgcaaa aaacaaggaa accccatgag catcaagtcc gacaaatgga tacgccgaat
     5281 gagcgaagaa ttcggcatga ttgatccctt cgagccgaac caaatcaaag aagccgacgg
     5341 acagcgcatc atctcctacg gtacgtccag ctacggctac gatatccgct gcgccaatga
     5401 atttaaaatt tttaccaaca tcaacagcac catcgtcgat cccaaaaact tcgacccgaa
     5461 aaatttcgtt accgtcgaag acgactgctg catcatcccg cccaattcct tcgcactggc
     5521 gcgcacggtc gaatatttcc gcattccgcg caacgtcctg accgtctgtt tgggcaaatc
     5581 cacctacgcc cgctgcggca tcatcgtcaa cgttaccccg ttcgagccgg aatgggaagg
     5641 ctacgttacc ctcgagtttt ccaacaccac ccccctgccc gccaaaatct acgcaggcga
     5701 aggcgtggcg caagtcctct tcttcgagag cgacgaagtg tgtgaaactt catacaaaga
     5761 ccgcaacggc aaatacatgg gacaaaccgg cgtaaccctg cccaaagcct gaaatacaaa
     5821 atgccgtctg aacccttatc gtcgggtttc agacggcatc tacctattta tccgtggtta
     5881 gaactgaacc gtcccattga tgctgatgct gatttcctcc acacccggtg cggctgaatc
     5941 cgtacccttc atattgacgc ttgccgccat cggcatcgcg cgcagcattt ttgcccgaac
     6001 agccccatcg cccgcaatat ggctgccgat ttgcccaaaa ttcaatttga cgattttata
     6061 accggacgca cccagaacgc ccgccagttt ttcggcacgc gccttgaaac gcaaaacggc
     6121 atccttgctg acctgatcga tgacctcgtt tcggcgttcg cgcgacacgc tgaaatcggt
     6181 atcttcaagc gaagcatccg tctgaacatc agcaataaaa cggtttaaag catcaaaatc
     6241 cctgccctcc gccttaaatt ccgcacgctc ctcccagcct gtttgaatgc gtctgccgtt
     6301 ggtatattga tagcgcggca tcgcactgcg cgataccaat tcggttttaa agctaccatt
     6361 tttcgatttt ctggtaaaat tgttgaattt tttaacaaac tcggcattga cggcattttt
     6421 gtcccgtcct tccgccgcca cctggaaacg cgcggacatt gtatcctgag ccacctcgat
     6481 acccgccgat tcggaaaatt cgacaatatt gtaattcaat gcctcagccg ccgccggaaa
     6541 agataccgcc agcagggaag ccgccaaaac agaacgcagc atatccgtat ccttacttga
     6601 aaaaccgcca atctaacata taggtacgct aaattgcgtt agcataagac aacaatttaa
     6661 atattcgatc ggtctgcctt ataataaccg tctttccgat actgaatcat attatgtccg
     6721 aaccgttgca cgtcctcgtc atccccttat ggtatccacg atccgaacag gatgtggacg
     6781 gaatattttt ccaaaatcaa gcacaggccc tccaacgcaa aggcatcaaa accgccatcc
     6841 tcgcccccat gttccgttat ctgcggaaag aaacagcaag catcctgacc ggtccttacg
     6901 gttttgcccg ataccggcaa agcggtttgg acatctacgc atggcgcagc atgtatttct
     6961 tccccgcttt ccgtttatcg acatcgaccg catccgctgg gtgcgcgccg gtttgaaagc
     7021 cttcaaacac tacatccgcg aaaacggact gcccgacctg attcacgccc actgcatgaa
     7081 ctatgcaggc atacttgccc aaaaaatttc cgaaaaatac ggcattccct acgttttaac
     7141 cgaacacagc agcaccatca cgcgcggtct gatccgtcac caccaatggc agccgatgga
     7201 aaaagcagcg gcgcccgcct ccgcacgtct cgccgtcagc cgccatttcg cacacgtcct
     7261 gcaacacaaa tacggctgcg aatggcagta cctccccaac ataccgggtg gaatattcaa
     7321 gcaaacattt gaataaaaag aaaaaaataa caaaccgcat ttcgtgttct gcaccgtctc
     7381 gcaccttcgc cgcctcaaag gacacgatgt cctgctcact gcttttgccc gggcgttggc
     7441 acaatgcccg caactgcgcc tgaacatcgg cggcagcgga caggaagaac agcggctgaa
     7501 acagcaggcg gcagacttgg gcatcgccca tgccgttaca tttttaggcg cactgcaacc
     7561 cgaagcaatc ttggatttga tgaggaacag cgacgccttc atcctcgcca gccgcaccga
     7621 aaccttcggc gtggtctata tcgaagccct ctcgcaaggg ttgcccgtca tcgccataca
     7681 ttgcggcggc gcggaatcca tcgtttcaga cggcaacgga tacctcgttt ccgttgacga
     7741 tgccgatgtc ctagcctttc tcaaaatata tgaacaccat gccgattttt ccgccgaaca
     7801 gctcagaacc gactgcctcg caacattcgg cgaagatgcc gttatcggca gactgattgc
     7861 cgtattccgt caggcggcgc ggaacacggc gcgaaaacgc ccgaaaaata gattaaaatg
     7921 acgcaaaaca accttcggat tcaaatatga cccataccgt ccacctgcac ttggaagaaa
     7981 ccgacaaccc gacgctgcaa cgcctgtgcg gttctttcga cagcaacctc gattcgctcg
     8041 ccaaagcact cgacatccat atcagccgcc gtttcgagca ttttaccttc aacggtgcat
     8101 ttgcacacgc cggcaaacgc gcactgctca aactcttgga aacggcgcag acgcgcgacc
     8161 taaacgacag catcatccgc cttgccgccg tcgaaaccca aaccgaagat gccgggcatc
     8221 aggaaaaaaa tcacgaacac gcctattatt tccgaaccaa gcgcggcagc ataggcggca
     8281 gaacgccacg gcagaacggc tatatccgcg ccctgctcga ccacgacatc gtattcggcc
     8341 tcgggccggc aggtacgggc aaaacctatc ttgccgtcgc cgccgccgtc gatgcgatgg
     8401 aaaaacacca agtcgaacgc atcattttag tgcggcctgc cgtcgaagcc ggtgagaaac
     8461 tgggcttcct gcccggagac ctgacccaaa aagtcgatcc ctacctccgt ccgctttatg
     8521 atgccctcta tgacctgatg ggctttgacc gtgtaaccaa gctgattgaa aaaggcctga
     8581 ttgaaatcac cccgctcgcc tatatgcgcg gcaggacgct caacggcgca tacatcatcc
     8641 tcgacgaagc gcaaaacacc acgcccgaac aaatgaaaat gttcctgacc cgcatcggct
     8701 tcggcgcgaa agccgtcatt accggcgaca ccagccaaat cgacctgccc aaaaacatca
     8761 aatcgggatt gaaagacgca cgtgagaaac tgcacggcgt ggcaggactg tatttccata
     8821 ccttcaccgg cgaagacgtt gtccggcatc ctctggtgca aaaaatcgtc gaagcctacg
     8881 aatcggcaga acacgactga cactccaatg ccgtctgaaa cccgatgcat ggaaaatcgg
     8941 gcagagaaaa acagagacgg aagccaagct ccgtatccgt ttctttatcc gtccaaccga
     9001 tacatcaatc gaattttctg catcggattg cctcatctcc gcaaaacatt gccgaagttt
     9061 cgcggttgca cgcctgacgc actttctgcc gcgccacgcc ttcattccga tcgcttgcct
     9121 caaacgtctt ctgaaaaggc gtaacgctgc atacatacat ggtggaattt gaaccgaaaa
     9181 ggtcgatttt gacggtattg ccgccgcttt ccaatacggc gacacggtgt tccaactgcg
     9241 ccagacgtgc ttccaaatcg ctgattctgt cggcaaatgc aggcatggcg gcacacgcgg
     9301 tcaaaatgcc caaaatacat tttttcatca atttttcctt gttgcttgaa ccctgcccac
     9361 ccggacatcc tttttccggg cggcagggtc agactttatt tgcagggatg aagcccctta
     9421 caaatcagaa taatacatga atcggcactt tatgcttcat cctatgtgtt gaaacttcat
     9481 tgctccttgt ctgttgaccg ttattcccaa tccgttccca caaccttcgg cttgggtttg
     9541 cgcctgcgac ggcggcgttt tttcgcctgt ccttcgtttt tttcgtgccg ggcggcggcc
     9601 tcgtttttgg tcatttccgt ccgctgttcc gccgatgccg tctgaaacgc cgtccaccac
     9661 tcggcaagtg cgcggtccgc attgccggtt tcggcgcgca agagcaggaa atcataggcg
     9721 gcacggaaac gcgcctgtgc aaacagtttg tgcggacgtg cgcctttgcg gttttcaaac
     9781 tgcggctgga acatccaaat ttcacgcatc gtggcggaaa agcgttgcgg cacgccccaa
     9841 ccgcgttcga cggtttcgcg catcgtattg attgcatcgg acagggcggg cgtaggtttc
     9901 aaaccctgtt gcagattgct tttccaatgg cggtccaact ctggccacat cagggccgcc
     9961 agtacgaaac cgaccgaaac cgatttgtcg gcacgcagcc gctcatcggt atttttcagg
    10021 gcaagcgccg tcatttttcc ggcgatgccg tctgaaacgc gcaaagcatt gagcagcgga
    10081 tggatgtccg gtatgtcaaa tccgttcaaa cgtttcagac actcgcgcgc gtgccctgaa
    10141 aacagcagtt tcatgatttc gtcgaacagc cttgccacag gttcgtgctt cagacggcat
    10201 atcgattcgg caatcggtgc ggcggtttct tccgacagct caaagcccaa tttgcccgac
    10261 aggcggatgg cgcgcaaaat cctgacaggg tcttcctgat agcgttcggc ggcattacca
    10321 atcataacca gcctgcgggc ggcaacatcg gcaatcccgt tgtggaaatc caaaatctct
    10381 tctttttcag gatcgtaata caaggcattg caggtaaaat cgcgccgcat cgcatcttct
    10441 tcgatgctgc cgtaggtgtt gtccttcatg attctgccgt gcgcgttctg atgaaccttc
    10501 gcaccgccgc gaaacgtcgt tacttcgata atctccgcac cgttcatcac atggacaatc
    10561 tgaaaacgcc tgccgatgat gcggctgcgg cggaagagtt tgtgcacctg ttcgggcgtg
    10621 gcatcagttg cgacatcgaa atctttgggt tcgatgccga gcagcaggtc tctgaccgca
    10681 ccgccgacca cataagcctg aaaccccgcc cctttcaggc ggcgtatgac gttttcggcg
    10741 gcaaagctca acatttcggc acggatgttg tgtctttcgg caggaatgac cgttttactt
    10801 tccgcttttt tactgctccg accggaaggc agcatcttat tcaaccattt tttcagcata
    10861 aatattctga cccgaagagc ggcatctcca cgggcaaccg tgttcagact gcatcaggcg
    10921 gtacgattcc acaagatgcc gaaaaccata agcaaaaggc gtagaaacta cgccgtccga
    10981 atcacgccgc cctcgcggcg gcaacgcgtc attataacag attgcccctt aaaaaatcag
    11041 accctgcttt tgtggcggag tctgaaattt gatgcacctt atatattgcg tccctaagaa
    11101 gggatgatta acaaaaatta acacccttta ctttcttttc cgattgggaa cattcccgtc
    11161 gtacaaacaa gcaaatgaag gaacagaaat cacattcaaa cggcgttccg ccgcctctac
    11221 gccgcttccc agtagtcgat ataaagctgc aattcgaggt tgtcgcgcca ttcgttggca
    11281 acggggcggt acaccgtgcg gatgtattcg ggaatgtctt cgctgcaacg ccagaacatc
    11341 gcttcaaatt cgcagccgtc tttttgcaac cacgcttttt tgtgtttgcc ttccgcgccc
    11401 aaaggctgct ggcgaacgac gtggaactcg tcggtaaagc tcggcggcgc aaagccctgc
    11461 ccccaaacgt ggcaggcgag gttttgcgcc tgttccaacg tgatgtcgca ggcgggcagg
    11521 ctgccgtcag taatataggt ttgcgacaaa tcgtcttcgc acaccatctc acgcacggct
    11581 tcttcaaagg ccgtctgaaa cgcgggaata ttgtgttcaa gtatgcttaa acccgccgcc
    11641 atcgcgtgtc cgccgaattt caaaatcaaa tcgggatggc gtttggacac caagtccaaa
    11701 gcatcgcgca agtgcagatt gggaatggag cgtcccgaac cgcgcacttc gccgttgtcc
    11761 gccggcgcaa acacgatggt cggacggtaa aaacggtctt tgaggcggct ggcaacaatg
    11821 ccgaccacgc cttgatggaa gtcgtcgcga tacgccacca aagtcgtctg acctgaaggc
    11881 agggtttcgg gaaaggcgtt caacgcgtct cgcagcatag actgctcgat ttcgcggcgt
    11941 tcgatattga ggttgtttaa ccgagccgcc agttcctgcg cttcggaatc atctcgcgcc
    12001 aacaggcagg cgatgccgac cgacatatcg tccagccgtc cggcggcgtt gatgcgcggg
    12061 cccaacgcaa agcccatatc aaacggctga gccttgcgcc aatcgcgccg cgccacttca
    12121 aacaaggcgc ggatgccggg gcgcattttg cccgaacgca tccgtttcaa accttgcgac
    12181 acgaggatgc ggttgttgtg gtcgaggggg acgacatcgg cgacggtacc gagtgcgacc
    12241 aaatccaaaa ggccgcccag attcggctct ttgatgccgt ctgaaaaata attgcggcgg
    12301 cgcagttcgg cacgcaacgc cgtcaacaca taaaaaatca cgcccacgcc cgccaagctt
    12361 ttgcttggaa aaccgcagcc tttttggttc ggattgacga tgatgcagtc gggtacggta
    12421 tcggctggca aatggtggtc ggtaacgatg acatccaaac ccagagcctg cgcgcgcgcc
    12481 acgcctgcaa tactggcaat gccgttatcc accgtaatca gcaaatccac gccttgcgcg
    12541 gcggcgattt cggcgagttc gggcgttaag ccgtagccgt gctcaaagcg gttgggcacg
    12601 aggaaatcca ctttcgcgcc catcgccgcc aaaccgtcca aaccgacggc acacgcggtc
    12661 gcgccgtcgg catcatagtc ggcgacaatc aggatttttt ccttgcgttc aaccgcatcc
    12721 gccaaacggc cggcggcggc ttcgcaattc gtcagcgatt gataaggcag gagggaagcg
    12781 agtttgtcgt ccaactcggc aggactttgc acaccgcgcg aggcacaaag ccgggcgatt
    12841 aaaggatcgg cgccggcggc gagcaaatga ttgaaaacgt cggtattgac ggggcgggtt
    12901 tggattttga ctgacatagc gcggggtttt acagtgcggg tgtgcttgaa atgccgtctg
    12961 aaggcttttc agacggcatt gccgttcatt tgtctgagga attcgcggct gcaatcagag
    13021 ccataggcaa gaccagcacc gcggccgccg catccaatat caacaagggg ggcgtatata
    13081 agatatttcc aaacagcttg gatttgtcgg tatgtttttc agtaaccgta taataaatat
    13141 cggcgggcac actttgctca aaatgataat cggcgttcag tttttgcggt gtggcgtagt
    13201 atttgccttt ggcggatacg cagcgcgtgt aaatggtccg attgtcgagt ttgaccgctt
    13261 taaactcaag ctgtttcagc ttggcgatgt cgtcaggtct gccggtatca tagcgcaggc
    13321 aaagacctcc ggtactgaaa ttctggctgc cgggcgcttc gaatttgacc ggcagggctt
    13381 ggtggcgggc atagctcggg gtatcctcaa ctatttggaa gggcttgtcc aacccggcct
    13441 tcaaaaggcc cgtcagcttc gccgaatctt cgggattgac ggcgaaccag tatttcccgc
    13501 ccatcatcac caggctgccc ttttccaatt gggcattgtc ttcggcaacc acaccgaagg
    13561 cgcggatttg gtctttgtca acgtgtttgc gggtgattgt ttggctgacc gggttgttca
    13621 tcccccgcaa catcatcgta cagccgttca gtatcaaggt tgccgccaaa acggcggcgg
    13681 tcgttttacg gaacaacatt ttatcttcca atctaaataa cggcggatgc cgtctgaaaa
    13741 tatgcctaaa tttccgcgcc cccgttttcc atcacggttt tcaattcttg aaacagggcg
    13801 cggaagtttt tcggcggttt gtcctgttcc tgttcttttt tggtgttgcg gacgagcgtc
    13861 ctcagcctgc ccgcgccggc gtttggaaaa tcggacataa actgcgtcag cgcgccctcg
    13921 tctgccaaca accgtacacg cgcccgttcc acgcgttgca aaaaggcgtt gtgcgccgca
    13981 tcgtcgccgc gcagcttggc aaggaacgcc tcgatgggcg cgggatcggt atcgcgcatc
    14041 agacgtccga taaattgcgc ctgacgtttg agcgcgccgt tggacgtaat ttttttatag
    14101 gtaacgacgg cctcgtacaa atcttcatcc aaaccgattt ttttcagcgt gtcgtttgag
    14161 agcctggtca gttccatacc caaatcctgc aaaccgttca tctgcttttt catttgggtt
    14221 ttgctgatcc actcgtcttc ttgttcaaac atcttgattc tcgaattttt tccaatagcg
    14281 tattttaaca gaatgccgcc gcgttcagac ggctttttgc accgcacgcc ctgcaagcgg
    14341 cacgcttttg cccctgccgc ccgaaacggt taaaatgccg caacgctcca ccaagaaaga
    14401 ttgttatgct gttcaaccac accgcttccg aactcctcga cctctgccgc cgcacgctcg
    14461 acttggcaaa agcagcgggc gcgaccgccg ccgaagccga cttcagcgaa tcgctcggac
    14521 aaagcgtcag cgtgcgcttg ggcgaaatcg aacaaatcga gttccagcag gacaaatcgc
    14581 tggacattac cgtttacgtc ggcaaacgca aaggccgcgc cggcaccgcc gacttctccg
    14641 aacaagccct gcgcgacacc gtccgcgccg ccgccgacat cgcccgccac accgccgaag
    14701 acggttgcgc cggacttgcc gaccccgagt tgatggcgca acacatcggc gaccccgacc
    14761 tttaccacga atgggatttg gatacggaag ccgccgtcgg cttggcaaaa caatgcgaac
    14821 aggcagcgtt ggacacggac agccgcatcg aaaactccga aggcgcggcg gtgcaaaccg
    14881 gacactacca atacgtttac ggcaacaccc acggttttgc cgcacaccgg caaagcacgc
    14941 accacagcat ttcctgcagc gtcgttgccg ccgacgaaaa cggaatgcag cgcgactact
    15001 ggtacgattc cgcctgccgc catccggata tggacagccc cgaaaccatc ggtcaaaccg
    15061 ccgcccgccg cactttgcgc cgactcggca gccgcagcat cccgacaggc agctaccccg
    15121 tcctcttcga taccaccgtt tcgggcgggc tgatcggaca cctcgtcggc acactctccg
    15181 gcggcgcgct ctaccgccaa agcagtttcc tgatcgacag catcggcaaa aaagtcctgc
    15241 ccgatttcct caacctgcgc gaagaaccgc acatcccccg ctctttccgc agcagctatt
    15301 ttgatgcgga aggtgttgcc accgcgccgc gcttcgtgat tcaaaacggc attgtcgaag
    15361 gctatttcct cggcagttac agcgcgcgca aactcggaat gcagaccacg ggcaacgccg
    15421 gcggcgcgca caacctgtat ttgaaccata cgcacgaaac gcaatccgac ctgctgaaag
    15481 aaatgggcac gggattgctg gtaaccgaat tgatgggaca gggtgcgaac accattaccg
    15541 gcgactattc gcgcggcgcg gcgggttttt gggtggaaaa cggcgtgatt gcctaccccg
    15601 tccacgagat taccgttgcc ggacgcttgc aggatatgta ccgcgacatc gtcggcgtgg
    15661 cggatgacgc tttgcggcgt tcgtccaaca aaatcggttc gattctgatt gcggggatga
    15721 cggttgccgg aagctgaacg ccctgccgat gccgtctgaa gacgaaggcg gcgcgttttg
    15781 ccccgtgcct gcccgtttgc cgttttatgc cgcaccgcct tttaccggcg acattgcaca
    15841 tttttttcat cagcactgat tttcccttat ccgtcaaaat actaagccac ttgcccacaa
    15901 cagggtacac acaagttcag gataaggcgg gcggggattg gggacttttc cgccatcggc
    15961 aaaacggcag acaaaaacgg cgggacattg cctgtcccgc cgttttcaaa tatacatttg
    16021 accgtacatc aggaattaag ctgcctgaat gttggcggcc tgtttgcctt tagggccggt
    16081 ggttacgtcg aaagagacgc gttggccttc tttcagggtt ttgaaacctt ccatattgat
    16141 cgctgagaag tgtgcgaaca aatcttcgcc gccttcgtca ggagtgatga aaccaaaacc
    16201 tttagcgtcg ttaaaccatt ttacgatacc ggttgccatt agaaacttcc tatacttaaa
    16261 aattaacaaa atcagcaaaa taaggcatac agcaaactta aacgttcagc gtttccaccc
    16321 agctttatat acttggtcga gtgccgactt ttgcttaacc gtctttttac atttgttaag
    16381 acaaaacgtc aagctatgtt ttcaaaaaag tgggaaaata ggcgaaagta ccgaaaatac
    16441 aacaaaaatg ccgtctgaac gcggctttca aatgggacac gctatgaccg cgcaacacca
    16501 atccgacacg cttttgcacc gcctgaacac cctgccgccg aaacgttacg gcgtattttt
    16561 attgaacgac gattacacca cgatggaatt tgtcgttgaa atcctgaccg aaatctttat
    16621 gctcgggcag gaacaggcgg tagcggtaat gctctcggtt catcacgaag gcaaaggcct
    16681 gtgcggcact tacacgcgcg atatcgccca aaccaaacag caacaagtca tgcagcgggc
    16741 aaaagccgaa ggacatccgc tgcaatgtat tgtcgaggag atttaatatg cttgcccctg
    16801 aattggaaca gattttgcag cagctttacc gcgaggcgcg taaggctcat tatgaattta
    16861 tcagcctcga gcatctgctt ttggtactca tcgaagaaga tgccgccgtg ccgaacgtct
    16921 taaaactctg cggtgcggat ttaaaagcgg tgtccgaaca gctcgccgcc agcgttgccg
    16981 aaaacacccc cctgattccc gaccaccttt tagacacggt cgaaacccgg cccacgctcg
    17041 gcttccaacg cgtcatccag cgggcgatgg tgcataccca atcggcaggc aaaggcttgg
    17101 ccgagccttt ggacgttttg gtcgcgctga tgagcgaaac cgacagccac gccgtttatt
    17161 ttttgggttt gcaatctgtt acgcgttttg aagttttgcg ctgtattgcc cacggctctc
    17221 ccgatgaaga tgaagacgat ggcaactatt tttcagacgg catggacgac gataatgaga
    17281 accgcaccaa accgggcaaa aaccctttat cggcgtacac cgtcaacctc aacgccgaag
    17341 tcaaagccgg ccgcatcgac cctttgattg gtcgcaaaca cgaaatggaa cggctggtgc
    17401 aaatcctgtg ccgccgccgc aaaaacaatc cgcttttggt cggcgaagca ggcgtgggca
    17461 aaaccgcgct ggcggacggt ttggcacatc aaatcgtcaa cgacgacatt cccgacgcgc
    17521 tcaaagaggc cgaagtgtac gcgctggata tgggttcgct tttggcgggc acaaaatacc
    17581 gcggcgactt tgaagcgcgg gtcaaatccg tcttgaaaca gctcgaaaaa atcccgcacg
    17641 ccattttatt catcgacgaa atccacacca tcatcggcgc gggcagcacc ggcggcggca
    17701 cgatggacgc gtccaacctg ctcaaacccg cattggcgaa aggttcattg cgctgtatcg
    17761 gcgcaaccac ttacgacgaa taccgtacta tttttgacaa agaccacgcc ctaagccgcc
    17821 gcttccagaa aatcgacgtg gtcgaaccca ccgttgccga aaccgttcaa atcctgcgcg
    17881 gcttgaaacc gatgttcgaa gccttccacc aagtccgcta cacgcaaggc gcactcgaag
    17941 ccgccgccga actctccgcc cgctacatca acgagcgctt cctgcccgac aaagccatcg
    18001 acgtaatgga cgaagcaggc gcggcgcaac ggattctgcc caaatccaaa cagaaaaaag
    18061 tcatcggcaa agcgcaaatc gaaaccgtca tcgccaaagt cgcgcggatt cccgaaaaga
    18121 ccgtgtcgca cgacgacaaa caagtgttgc aattcctcgg ccgcgatttg aacaatatgg
    18181 tttacgggca ggaagacgcc atcgacgcgc tggtttccgc cgtcaaaatg tcgcgttcgg
    18241 gcttggggct gcccgacaaa ccgataggca gcttcctctt ctccggccct accggcgtcg
    18301 gcaaaaccga agtcgccaaa cagcttgcct actcaatggg cgtaccgctg caacgctttg
    18361 atatgtccga atacatggaa cgccacgccg tatcgcgact catcggcgcg ccaccgggct
    18421 acgtcggctt tgaacaaggc ggccttttga ccgaagccgt caacaagcag cctcattgcg
    18481 tgttgctctt ggacgaaatc gaaaaagccc atccagatat tttcaacgtc ctcctgcaag
    18541 tcatggacgc aggcaaactg accgataaca acggtaagag cgccgatttc cgcaacgtca
    18601 tcctgatcat gaccaccaac gcaggcgcag aaagcctcag ccgacccagc ctaggcttta
    18661 ccgccaaacg cgagcgcggc gacgaaatgc aggcaatcaa caaactcttc acgcccgagt
    18721 tccgcaaccg cttggacgcg atcatcccgt ttgcaccttt gtccgaaccc atcatcgtca
    18781 aagtcgtgga caaattcctg ctccagctcg aacaccggct cctcgacaaa aaagtcgaag
    18841 ccgaattcac accggcattg cgtaaatact tggcagaaaa aggtttcgac ccgcaaatgg
    18901 gcgcgcgccc gatgcaccgc ctgattcagg aaaaaatccg caaaccgctc gccgacgaac
    18961 tcctgttcgg caaactcgcc gacggcggct ttgtgcggat agactgggat gcggcgaaag
    19021 aagaagccgt attgaagttt aagaaaagca aggtcaaaat aaaaaccgcc tccgcataaa
    19081 atcaaaaatg ccgtctgaaa ggttcagacg gcattttgtc aaatatcggc ccctattccg
    19141 gcaaacttct ccacaaacgc cgcgattttt tggaagacgc tttgtttctt cgtcagatat
    19201 tgcgggttga gcggactcat tttcggcagg gtttcggtca gttccgtacc gttttcgctg
    19261 gcatagccgc gtttgagcga gccggtcaaa tagcgtttgg cggcggtttc gttcagacct
    19321 tcggcggcaa tcaatcctgc cgcttcgtgc cgcatcacct cttgcgcgta ggtgtaaaag
    19381 gtttccagta tggcgggaac gtcgggcact ttatccaaat ccgtatcgtt gatgaaatcc
    19441 acaatcaggc cctctttggc gcggtggccg atgctggcgc ggatgatgcg gcggatttct
    19501 tccaccagct ccgctttgcc tttgattttt ttgtgatgct cgaaaaccag ttgcaggatg
    19561 taatccagat tgatttcctg cgatttgagc aaatccacct caaaaaccac atcgtcccag
    19621 tcgatttttg actgttcttt ctgttcgcct gctttttggc ggcgcagcca gtctcggatg
    19681 tcgttatagg cggaacggta gtcctgcacc gccctttcag acggcatcgg cactttccgc
    19741 atttcctgca cgtcttcgtc gctcaggtag tatttttcct gaaacgcctt catcgcatct
    19801 tcgtccgccg cgtccacatt ctgcaattcg cgcagcgcgg caaattcatc gtagttctgc
    19861 aatacatttt ccgcccgcag gtattcgccg aagagtttgg caaaatcttt tttgtctttt
    19921 tccgtttcga ttttgtcggg atcggggaaa cgctcgtgca attcttttgc tacatccaga
    19981 taaccgcgcc gtgcttcgcc ggtctggctg tcggtatagc cgttcatgta ttcttcgtag
    20041 cttttttcca gcactacatt tttggtgttt ttgtcgccga agagcgtgat cgcatcaatg
    20101 gttgcctgct ccaagtcgcg gaagcagaca atgttgccga aggttttggt agcatcgtaa
    20161 atgcggttgg tgcgagaaaa cgcctgcatc aggccgtgat agcgcaggtt tttatcgacg
    20221 aacagcgtat tcagcgtcgg tgcgtcaaaa cccgtcaaaa acatgccgac cacaatcagc
    20281 aagtccactt cccggttttt cacccgtttt gccaaatcgc ggtagtagtt ctgaaaggct
    20341 ttgctgtccg tgccgaaatt ggttttgaaa caggcgttgt aatcgttgat gacagcctgc
    20401 aaaaattctt tcgcgctgct gtccatcgct tccggttcaa agttctcatc gacaatttca
    20461 ccgacggcgt tttgctcttc attggccgca aaggaaaaaa tggtggctgt tttcaacggg
    20521 tgcaggctgc ctgcctgttg tgttttgaac gcttcgtaat agcacttcgc cgcatccacg
    20581 ctgctgacgg caaacatggc gttgaagcct ttgccgcccg cattcaagcg gtgcgttttc
    20641 tgcctgaacc ggctcaggat atattgcgtg atttcgcgga tgcgttcggg gtgcagcagg
    20701 gcttggcggt tttcggcggc actcagttcc ttctcgtcct gttccgcttc cacggctttg
    20761 aactgcgggc gcacatcgtt gtaatccact ttgaatttca atactttttc atcgcggatg
    20821 gcatcggtaa tcacataaga atgcagctcc cgcccgaaca cgcccgccgt ggtttccgcg
    20881 cccaaagcgt tttcgggaaa aatcggcgtg ccggtaaagc cgaactggca gaatttttta
    20941 aatttctttt tcaggttttt ttgcgcttcg ccgaattgcg agcggtggca ttcgtcgaaa
    21001 ataaatacaa cctgccggtg ataaaccggc agattatctt cacttttcat caggttgttc
    21061 agcttttgga tggtggtaac gatgattttg ttgccgtctt tttccaaatt gcgtttcaag
    21121 cccgccgtac tttccgagcc gtttacgctg tcgggcgaaa aacgttggta ttccttcatc
    21181 gtctgatagt ccaaatcctt cctgtccacc acgaagaaaa ccttgtcgat aaacgccgat
    21241 tccgtcgcca gacgcgccgc cttaaagccg gtcagcgttt tgccgctgcc cgtggtatgc
    21301 caaacatagc cgccgctttc cggcccgctc caattcttcg cctgcgccga gctgttgatt
    21361 ttccacaaaa tgcgttcggc ggcggcaatc tggtacggcc gcataatcag cagcgtatca
    21421 ttcgcatcga acacgctgta atgcagcaaa acgctcagca atacgctttt ctgcaggaac
    21481 gtggcggtaa agtctttcaa atccttaatc ggatgattgt ccgaccgcgc ccaattcatc
    21541 gtgaaatcga agctgttttt gtcgcgcttg gtggtgttgg cgaaataacg cgtgtccgtg
    21601 ccgttggaaa tcacgaagat ttgcaggaat ttgaacagcg aattttcgct gttgaagctc
    21661 tctttgctgt aacggtgcac ctgattgaat gcctcgcgca ccaccacgcc gcgctttttc
    21721 aattcaatct gcaccagcgg caggccgttt accaacacgg taacgtcata acggtttgca
    21781 tgcgtgcccg tctgctcaaa ctggttgata agctgcacat ggttgcgggc aaggtttttc
    21841 ttgtccagca gataaatgtt tttcagacgg ccgtcatcaa aagcgaaatc gtaaatatgg
    21901 tcgtcgtgga ttttgcgggt tttatcggta atgttttcag ccggcctgtc cagatattcc
    21961 gtcaaaaacc gcgcccattc gccgtctgaa aacgccacat cgttcagccg ctgcaactgc
    22021 gcgcgcaggt tttccagaag cctgctttgg ctgttcaaat ccttgcggta ttcgtacccc
    22081 tgattctgca aatcggcgat taactccgct tccaaccggt tttccgattg gtagctgccc
    22141 gactgttcga ttttttcata ttggtcgagc acgatgaaat tcggcgtttc ggcgatgggt
    22201 ttggtttcga ggttcatggg tcgtcctttg cggtgtcggg ttttatagtg aattaacaaa
    22261 aaccggtact gcgttggctc gccttagctc aaagagaacg attccctaag gcgcccaagc
    22321 accaagtgaa tcggttccgt actatttgta ctgtctgcgg ctcgccgcct tgtcctgatt
    22381 tttgttaatt cactataaac ggtttgatgt tcggattggg ctgcctgaaa aagtgcaggc
    22441 tgctttttca agcagccttg gggaaggcaa gcagctgttc gcggtaatat tcgtattgtt
    22501 tgcggcgcag ggcgatttcg tgcggcaggc cttcgctgac ggagtgggtc agggtatcga
    22561 atttgcctag gatggcgacg attttttcct gttcggggag cggggggatg gggattttat
    22621 attgcataat cgctgctttg ctgccacgcg gcatttttgc acctttggcg tgcttcatat
    22681 taaaagcaaa aaatttatca tcggccaata cctgatacag atattttgga ttaacctttt
    22741 catctgttac ccggataacc aaaacatcac cgtttgttcc gcccgtgcag tctgcctgcc
    22801 agattttttt caaatacgga cggatatttc caatcaaaat gtcattgaca atatattctg
    22861 tcatttttcc ttcgcttgga acataaccgg ataacttttt accttctctg ttttgtaaga
    22921 gattatccac gccaacgtaa ttatgttcgt tcagtttatc cgaacaaata cggttttttg
    22981 aatattcggc aacctccccc aacgtcttcc aaaccacatt tttcagacgg cattgatagc
    23041 cgtcagctat cccccccgat ttgattgtca aaatctaaaa gtaagtcgcg gtaataccgg
    23101 tattggcgtt tgcgcagggc taattccgct tccagcgtag cttccagcgt agcttccagc
    23161 gtagcttcca gctcggtgaa tttgtcaagt atttttacaa ttttttgttg ggtttccaat
    23221 gatgggatgg ggattttata gttatctgta tctggtgttg ctatttgtgg catttgcatt
    23281 tttgatccga tattacgaaa atagttttct tgtgttttta agaaataata aacatatttt
    23341 atataaatat gtttattaac tgaatgataa gaccacattt catttttatg cgaaaatggt
    23401 ttatcgtaat attcgaattc aataatacct cttgatttaa cgataataga aggctctcta
    23461 tggatatcct tatcaggaac atcatcaaag tcaactaaat gcaaaagttt ttcctcctgc
    23521 aaaaatcttt aacggcgcat tatctttatg catttctttc atttgtccag cagttatctt
    23581 agttcctttt gtacgaacta aaacctcccc caatggcttc cactccaccg gtgccgtctg
    23641 aatcatctca atcaattttt tcgctttgct ttgcatatcc atggttgttt tccgtttcct
    23701 tcccgctgcg gcgggatatg gggcggttgc ggggcgggtt tcagcccgct atgtccgttt
    23761 gccgttattt ctgcgggctg aagcccgtcc tgctttcagg ctgcctgaaa tcgtgtgcag
    23821 gctgcttttt caggtagcct gaatgcggtt attttttgcc gttttgatgc tgttcggttg
    23881 cggcaagcct tttctgttct tcgttctcaa gctgtttgat gcttttctcg ggtacgggct
    23941 ggttttcagg catgatgccg cccagttctt caatggtttg gcgcactttt tgtccgactt
    24001 cgaaatgcac ccggtttgcc tgtgttttgc cttggatgtt tttgcggcgt agtttttctt
    24061 cggtttgcgt ggcacgaaac agattggcag ccggttcgct ggcgttcatg tggtctagga
    24121 ttctctggct tttcttcaag cctttgcggc tgtgtatgcc ctgcttgtcc agtcctccgt
    24181 atagcccccg atagccgtgg ttttggaaga cggcgtactc aacgggtttt tctacgcctg
    24241 catccttggc tgcggcggca aggtcggtat
//
LOCUS       DAXDSD010000021        22988 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXDSD010000021 DAXDSD010000000
VERSION     DAXDSD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22988
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.021"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(42..380)
                     /locus_tag="ACFB2Q_000658"
     CDS             complement(42..380)
                     /locus_tag="ACFB2Q_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC0917733.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            531..4487
                     /gene="purL"
                     /locus_tag="ACFB2Q_000659"
     CDS             531..4487
                     /gene="purL"
                     /locus_tag="ACFB2Q_000659"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC0917734.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            complement(4558..5769)
                     /locus_tag="ACFB2Q_000660"
     CDS             complement(4558..5769)
                     /locus_tag="ACFB2Q_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFC0917735.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARMQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(5843..6154)
                     /locus_tag="ACFB2Q_000661"
     CDS             complement(5843..6154)
                     /locus_tag="ACFB2Q_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC0917736.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            6386..7138
                     /gene="gloB"
                     /locus_tag="ACFB2Q_000662"
     CDS             6386..7138
                     /gene="gloB"
                     /locus_tag="ACFB2Q_000662"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC0917737.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGNGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            7372..8826
                     /gene="mgtE"
                     /locus_tag="ACFB2Q_000663"
     CDS             7372..8826
                     /gene="mgtE"
                     /locus_tag="ACFB2Q_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC0917738.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(8912..9820)
                     /gene="hslO"
                     /locus_tag="ACFB2Q_000664"
     CDS             complement(8912..9820)
                     /gene="hslO"
                     /locus_tag="ACFB2Q_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC0917739.1"
                     /translation="MNQTAINRADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            10065..10646
                     /locus_tag="ACFB2Q_000665"
     CDS             10065..10646
                     /locus_tag="ACFB2Q_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC0917740.1"
                     /translation="MDSFFKPAVWAILWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPVNRAPARRAGNADELIGSAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGAPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRIKKNDPSRFLN"
     gene            10665..10883
                     /locus_tag="ACFB2Q_000666"
     CDS             10665..10883
                     /locus_tag="ACFB2Q_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917741.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            11028..11372
                     /locus_tag="ACFB2Q_000667"
     CDS             11028..11372
                     /locus_tag="ACFB2Q_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC0917742.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            11372..12064
                     /locus_tag="ACFB2Q_000668"
     CDS             11372..12064
                     /locus_tag="ACFB2Q_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC0917743.1"
                     /translation="MSEILRQPGILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFRLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLPLASKSVTNPIAIEITSSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            12131..13351
                     /gene="argJ"
                     /locus_tag="ACFB2Q_000669"
     CDS             12131..13351
                     /gene="argJ"
                     /locus_tag="ACFB2Q_000669"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC0917744.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            13411..14820
                     /locus_tag="ACFB2Q_000670"
     CDS             13411..14820
                     /locus_tag="ACFB2Q_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC0917745.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWIALPPGLPVIVWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLENILMWIVAAGLVCGAAGGIFARMLHRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPVGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRRRVLQETAAQTG
                     NAPARPQAANSKTGMPSEN"
     gene            15008..16399
                     /gene="hrpA"
                     /locus_tag="ACFB2Q_000671"
     CDS             15008..16399
                     /gene="hrpA"
                     /locus_tag="ACFB2Q_000671"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC0917746.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDDTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEEN"
     gene            complement(16761..17447)
                     /locus_tag="ACFB2Q_000672"
     CDS             complement(16761..17447)
                     /locus_tag="ACFB2Q_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917747.1"
                     /translation="MENKQTEKTETQSEKTKKKPFKQTRELVRLALNNGWTQTEIAKT
                     CRTQQSVVSAWSKGEKLGTEQQLKPLLDLFGYKLRRNTFKLYWRKNAETSKIEFIKVE
                     GKIIFSLLIGKNNGYNKFVPAYKFIIHYQGNEQFYMILKKKIYDDYDKSLEAAAWQTI
                     LNQSIGISELLRFFDKLSSKDIENMGRFWLEFCCNFPKYTQSLPFLIRQALLNHGFSV
                     NDIIEYQASW"
     gene            complement(17518..17670)
                     /locus_tag="ACFB2Q_000673"
     CDS             complement(17518..17670)
                     /locus_tag="ACFB2Q_000673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917748.1"
                     /translation="MSNKQANHQSNMSNANKGTSGTNKTYSQNQGNRGWQMNPQNPAN
                     SGKGKK"
     gene            18131..19708
                     /gene="lpt3"
                     /locus_tag="ACFB2Q_000674"
     CDS             18131..19708
                     /gene="lpt3"
                     /locus_tag="ACFB2Q_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFC0917749.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FMIAALYLFARYKASRLLIAVFFAFSMIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIADK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHDQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRNGKAEYVYPQ"
     gene            19810..>22988
                     /locus_tag="ACFB2Q_000675"
     CDS             19810..>22988
                     /locus_tag="ACFB2Q_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC0917750.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLTYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAGYEQIHRALLTGL
                     IANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLAVLPRRPVSYGKVAPEEAR
                     EIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYHERLP
                     DFYMADSVSEGLCPANPQQTTPSPVGEGWGEGKTVAAQTNFSATSASPLPNPLPQERE
                     QSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPPFSDGLRPANPQQ
                     TTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDDPKPKKQPASQKG
                     RLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEEQFPKHWQTADGK
                     FKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQLQIKALPKQIRR
                     ICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQDEWAAFRLPEHC
                     YFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDNVTAWDIGILPES
                     IKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIELMKLQLKEQVKDL
                     NKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEKAFKEQIKRARSR
                     LPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPGFATRTPWAQWPR
                     LPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQGQPVSDGLAAFK
                     WMIEELRVSLFAQELKTPYPVSVKRLLKM"
BASE COUNT         5426 a         6767 c         5724 g         5071 t
ORIGIN      
        1 ccgtctgaaa gcctttcaga cggcatttcc attccaaacg ctcaaaccgc cgcgtccgaa
       61 cgttcgcccg tgcggatacg gattgcctcc tcgaccggca gcacaaaaat cttgccgtcg
      121 ccgattttgc ccgaacgcgc cacctcgaca atcacgtcaa tcgcgcgttc cacggcatca
      181 tccgccaaca ccaactcgat tttgaccttg ggcaggaaat cgacggcgta ttccgcaccg
      241 cgatagattt ccgtatgccc cttctgcctg ccgaaccctt tgacctcgct gacggtcatg
      301 cccgtaatgc cgatttccgt caacgcctcg cgcacgtcgt cgagcttgaa cggtttgaca
      361 atcgcctcga tttttttcat aaaatttcct ttgaacaaac aatacaaaca catccgaaaa
      421 acgggaaccc ccgtcagatt gtcaacattt taaaccaaaa tacccaagca atacagcccc
      481 cgttgcgcat ataatgacag cttttccaac cgcatttgag agccgaatcc atgtctgttg
      541 ttttgccctt gcgcggcgtt accgcccttt ccgatttccg tgtcgaaaaa ctcttgcaaa
      601 aagccgccgc actcggcctg cccgaagtca aattaagcag cgaattttgg tatttcgccg
      661 gcagcgagaa agcacttgat gccgcgactg tcgaaaaact gcaagccttg ttggcggcgc
      721 aaagcgttga acaaacgccc gaagcgcgcg agggtttgca tttgtttttg gtaacgcccc
      781 gtttgggtac gatttcgccg tgggcttcca aggcgaccaa tatcgcggaa aactgcggtt
      841 tggcaggcat cgaacgcatc gagcgcggta tggcggtgtg gctggaaggt gcgcttaccg
      901 atgaacagaa acagcaatgg gcggcgttgc tgcacgaccg catgaccgaa tccgtgctgc
      961 ccgattttca gacggcatcc aaattattcc accatctcaa atccgaaacc ttctccaccg
     1021 tcgatgtttt gggcggcggt aaagaggctt tggtcaaagc caataccgaa acgggtttgg
     1081 cactctccgc cgacgaaatc gattatctgg ttgaaaacta tcaggctttg cagcgcaatc
     1141 cgtccgatgt ggagctgatg atgttcgcgc aggcaaacag cgaacactgc cgccacaaaa
     1201 tcttcaacgc cgatttcatc ctcaacggcg aaaaacaacc gaaatccctg ttcggcatga
     1261 tacgcgacac gcacaacgcg catcccgaag gtacggtcgt cgcctataaa gacaattcgt
     1321 ccgtgatcaa aggcgcgaaa atcgagcgtt tctatccgaa tgcagcggaa aaccaaggct
     1381 accgtttcca cgaggaagac acccacatca tcatgaaagt ggaaacgcac aaccacccga
     1441 ccgccatcgc gccgtttgcg ggcgcggcaa caggcgcggg cggcgaaatc cgcgacgaag
     1501 gtgcgacggg caaaggttcg cgtccgaaag cgggcttgac cggctttacc gtgtccaacc
     1561 tcaacatccc cgacctcaaa cagccgtggg aacaagacta cggcaagccg gaacacattt
     1621 cctcgccgtt ggacatcatg attgaaggcc cgattggcgg tgcggcgttc aacaacgaat
     1681 tcggccgccc gaacctcttg ggctacttcc gcacctttga agaaaaattt gacggtcagg
     1741 ttcgcggcta ccacaaaccg attatgattg ccggcggttt gggcagcatt caggcgcagc
     1801 agacgcacaa agacgaaatc cccgaaggcg cactgctgat ccaactgggc ggcccgggca
     1861 tgctcatcgg cttgggcggc ggtgcggctt cttcgatgga taccggtaca aatgatgcgt
     1921 ctttggactt caactccgta caacgcggca accctgaaat cgaacgccgc gcgcaggaag
     1981 tcatcgaccg ctgctggcag ctcggcgacc aaaacccgat tatctccatc cacgacgtgg
     2041 gcgcgggcgg cttgtccaac gctttccctg aattggtcaa cgatgccgga cgcggcgcgg
     2101 tattcgagct gcgcgaagtg ccgttggaag aacacggttt aacgcctttg caaatctggt
     2161 gtaacgaatc gcaagagcgt tatgtcttgt cgattttgga aaaagatttg gacaccttcc
     2221 gcgccatctg cgaacgcgaa cgctgcccgt ttgccgtagt cggcacggcg accgacgacg
     2281 gtcatttgaa agtgcgcgac gatttgttct ccaacaaccc cgtcgatttg ccgctgaacg
     2341 tcttgctcgg caaaccgccc aaaaccacgc gtaccgacaa aacggttacg ccgtccaaaa
     2401 aaccgtttca cgcgggcgat atcgacatca ccgaagccgc ctaccgcgtt ttgcgcctgc
     2461 ctgccgtagc cgccaaaaac ttcctgatta ccatcggcga ccgcagcgtc ggcggcatga
     2521 cccaccgcga ccaaatggtc ggcaaatacc aaacccccgt agccgactgc gccgttacca
     2581 tgatgggctt caacacctat cgcggcgaag cgatgtctat gggcgaaaaa ccggccgtcg
     2641 ccctgtttga cgcgcccgcc tcgggcagaa tgtgcgtcgg cgaagccatc accaacatcg
     2701 cggcagtcaa catcggcgac atcggcaaca tcaaactttc cgccaactgg atggcggcgt
     2761 gcggcaacga aggcgaagac gaaaaactct accgcaccgt cgaagccgtt tctaaagcct
     2821 gccaggcatt ggatttgagc attcccgtag gtaaagacag cctgtcgatg aaaaccgtgt
     2881 ggcaggacgg cgaagagaaa aaatccgtgg tttcgccgtt gagcctgatt atttccgcgt
     2941 tcgcgcctgt taaagacgtg cgaaaaaccg ttacgcccga attgaaaaac gtcgaaggca
     3001 gcgtattgct gtttatcgat ttgggcttcg gcaaagcgcg catgggcggc tcggcgttcg
     3061 gtcaggtgta caacaacatg agcggcgacg cgcccgattt ggacgatgca ggccgtctga
     3121 aagcgtttta cagcgtaatt caacagcttg tcgccgaaga caaacttttg gcgtatcacg
     3181 accgcagcga cggcggtttg tttgcgacgc tggcggaaat ggcgtttgcg gcacggtgcg
     3241 gtattagtgc cgatatagat tgcctgatgg ataaattcct gccgattcat ctcccggatt
     3301 ttcaaggcga ccctgccgaa gacttatctg acgaacttta taatcatgcc gccattaaaa
     3361 tcttattcaa tgaagaatta ggcgcggtta tccaaatccg ccaaaaagat agggattatg
     3421 ttgatgcggc atttgaaacg gccggcttaa ccgatgcggt cagccggatt ggctctcctg
     3481 attttgacaa tgagtttatt tctttctttg gttacggtta tttcttggaa caaaaccgcg
     3541 ccgacctgca acgcgcgtgg caggaaacca gccacgccat ccaacgcctg cgcgacaacc
     3601 ctgcctgcgc cgacagcgag ttcgccctga ttggcgacaa cgaacgcagc gcattgtttg
     3661 ccgacgtgaa gttcgacgtg aacgaagaca tcgccgcgcc gtttatcaac agtggcgcga
     3721 aacctaaaat cgccatcctg cgcgaacagg gcgtaaacgg gcaaatcgaa atggccgccg
     3781 ccttcacccg cgccggattc gatgcctacg acgtgcatat gtccgacctg atggctggcc
     3841 gcttccgcct tgccgacttc aaaatgctgg cggcgtgcgg cggcttcagc tacggcgacg
     3901 tactcggcgc gggtgaaggc tgggcgaaat ccatcctgtt ccaccctgcc ttgcgcgacc
     3961 aattcgccgc cttctttgcc gatccgaaca cgctgacatt gggcgtgtgc aacggctgcc
     4021 aaatggtcag caaccttgcc gaaattatcc cgggcgcaga aacatggccg aagttcaaac
     4081 gcaacctgag cgagcagttt gaagcgcgcc tgaatatggt tcacgtcccc aaatcagcgt
     4141 cgctgatttt gaacgaaatg caagactcca gcctgcctgt cgtggtcagc cacggcgaag
     4201 gccgcgccga cttcgcgctt cacggcggca atatttccgc cgatttgggc atcgcgctgc
     4261 aatatgtgga cggtcaaaac caggttaccc aaacctatcc gctcaacccc aacggctcgc
     4321 cgcagggcat cgccggcatt accaacgccg acggccgcgt taccattatg atgccccacc
     4381 ccgaacgcgt gtaccgtgcc gcacaaatga gccggaaacc ggaaggctgg acggaactgt
     4441 ccggctggta ccgcctcttt gccggcgcac gtaaagcctt gggctaaacc gtctgcccaa
     4501 acaaatgccg tctgaaaaag gttttgaaac ccgtttcaga cggcattttg accattctca
     4561 agccctgccg gtactcaatc ccgctttacc gttttcccgc aaagttttca gcaatacttt
     4621 ccacgccgac aatttagggt tggactgttt tccttgtccg atcaaatcca tttgcgtcat
     4681 ataccagttc atttctaaca ttgcacccgc ttttttatca acaaatgcta cgccagtttg
     4741 gatgcggtct gcttgtacgg tttggtaagt tctgttttgc attttgttcg ccaaatcagg
     4801 gactaaggca aacgggcggg ctatgccgac caaatccaaa tgaccgctgg ataaggcatc
     4861 ttccatggcg gtttgcgaac ggaatccgcc ggtgataatc agcggggctt ggctggctgc
     4921 acgggctttc tcggcgtaat cgatgaagaa ggcttcgcgt ttgcgggtgc tgtctttggc
     4981 ggcgagcatt tgcgggcttt cgtagttgcc accggaaact tcaataaaat caatgcccat
     5041 ttccgacagt ttttgcacca cctgcaccga ttcgctttcg tcaaatccgc ctttttggaa
     5101 atctgccgaa ttgagtttta cgcccaccaa gaaatctttg cccgcggcag cgcgaatagc
     5161 ggtgtaggtt tccaaaagga agcgcatacg gttttccaaa ctgccgcccc attggtcttg
     5221 gcggcggttg tggtgcggcg agaggaattg gctgatgaga taaccgtgta cggcgtaaat
     5281 ttgtacgcct gaaaagcctg cctgttcggc aattttggca gtttgtacga attgctgaat
     5341 cagcccgttg atttcatcgg cactcaattc gcgcggcgga ttgataaagc cattcatgcc
     5401 caccagcggc acggcgctcg gtgcaagcgg tgttttattg accaccgcag gcgactgttt
     5461 gcccgcatga ttgatttgca tgatgagcag cgtgtcgttt tgcatgcggg ctttcgccca
     5521 ttttttcagc atttcaaggg cgcggtcgtc tgaaatcaac acatcgttta tcgaaccttt
     5581 gccgctttca gccaccatca cattgcccgt caccaaaacg cctgcgccgc cttctgccca
     5641 agcgccgtac aggcggacaa gtttttcaga cggctggtcg ttttgggcaa gttgctcttc
     5701 catggcggat ttgaagatac ggtttttggc ggttttgccg ttttgaaaag tgaatggcgt
     5761 gaataacata atcgttcctt taagttgata agtttaaaaa tgtaaacgta tttgggtaaa
     5821 aataaagggc caacggataa ccctattttg aaagcgggca attttgagcc atcaaatcgg
     5881 caagctgttt caatctcgcc accgcatccg ccatggcaat actgtaaaac cgctctttgc
     5941 cgacctgctc caccgccacc gctccggctt gcagcatgat ttttaaatgg tgcgacacag
     6001 cagggcgcga cagatgcaga tgctcggtca gctcattcac attcatccta ccgtgtttcc
     6061 acaatacatg caggatctga tggcggtttt catcgctcaa tacggtaaaa atgggtatgc
     6121 actcgcgcat taaattcatg gtttgttgcg gcatattcga tctattgagt ttatatattt
     6181 aaaaattcga acgtatttta gagggaaggt cggcagttat caaaggcagt ttggtttgca
     6241 gtttggcaat atattgttgc tgaaaggttt ttataagaaa aaatgccgcc tgaaacagca
     6301 tgatggcgtt tgctagggtt ccgctgtata ttaacgcctt tcggatacac ctttcagacg
     6361 gcatccgaac catcaaggaa cactcatgaa aattaccccc gtcaaagccc tgaccgacaa
     6421 ctacatctgg atgatacagc acggcaacca tgccgtctgc gtcgatcctt ccgaaccctc
     6481 gcccgtcttg gaattcctcg tacgcaaccg actcatgctc gcccaaacct gggtaacgca
     6541 cccccatccc gaccacgaag gcggtgcggc ggcactctgg cgcggctaca tggaatcgcc
     6601 cgtttacggc gaatccgaca tcgaagccgc cacccacacc gtaaccgccg gcacacgatt
     6661 caccttcggc aacggtcagg ttaccgtttg ggcaacaccc ggccacacag accgccacac
     6721 cagctacctt ctcgaaactt cagacggcat acacgttttc tgcggcgaca cccttttttc
     6781 cgccggctgc ggacgcgtgt ttaccggcac ggtcgaacag ctttacgaca gcttccaacg
     6841 gttcaaccaa ttacccgaag gcaccctgtt ctatccggca cacgaataca ccgccgccaa
     6901 cctgcgtttc gccgcccata tcgagccgga caacgccgac attcagacgg cattgaaggc
     6961 ggcggaacgc acgcccaccc tgcccgttac cctcgcgcac gaacgccgcg tcaacccgtt
     7021 cttacggaca gaaatccccg ccgtccgcca acgtgccgaa gccctggtcg gcaaaacgct
     7081 gaacagcggt ttggaagtat tcgccgccct gcgcgaactg aaaaacgcct accgctgacc
     7141 tgccctccga aaaatgccgt ctgaaacccg cgtttcagac ggcatttgcg ttaaaaatag
     7201 taaaccgttt caaaagggag tagaatagtg ctgtttccaa tcccgtgcct gatttttcgg
     7261 gcttttatta tggaccttcc cagttcgttt ttactgaaca ccccttccga ttccaaacga
     7321 caataaccat cccgccggaa tgcctccccg cacacggcgg gcggagcatt tatgagcatc
     7381 gaacacactc ctccgacaca cgacggcgaa accggtcaaa accatgccga acgcccttcc
     7441 gccgatttcg accgtgtcca ctccctctgc gaaatcctcg aacctgcttt tgaacaaatc
     7501 gaaaacggca caccgctcga agatgcgccg ctgcgcgaca aactgaccga gctgaccgtc
     7561 ctcttggccg agttgcaccc tgccgacgtg gccgacgtct tggagtccct gccgccgcgc
     7621 gaacgcaata tcgtttggct tctggtcaaa cccgaagacg acggcgaagt attgctggaa
     7681 gtatccgacg cggtgcgaga aacgctgatc gagtcaatgg acaaggacga attgttggcg
     7741 gcggtcgatg atttggatgc ggacgaattg gcggagctgg cagacgattt gccgcaccaa
     7801 gtggtttatg aagccttaca gacgcgcgat gaggaagagc gcgcccaagt caaggcggca
     7861 atgtcgtatg aagacaacca agtcggtgcg attatggact tcgagttggt cagcatccgc
     7921 gccgatgtcg cctgcgaagt ggtgctgcgt tatctgcgcc gcttcgacag cctgcccgac
     7981 cataccgaca agatttttgt cgtcgatgaa aacgacgtgc tgcagggcgt gctgcccatc
     8041 cgcaaacttt tggtcgccga tcccgaagat ttggtggaaa acgtgatggc gaaagatgtc
     8101 gtgcgtttcc gcgccgagga cgacgtggaa gaagcggcgc aggcgtttga acgctacgac
     8161 ttggttaccg cgcccgtcgt tgatgaaaac aaaaagctca tcggcaggat taccatcgac
     8221 gagatggtgg acgtgatccg cgaagaatcc gaagcggata tgttgaacat ggcaggtttg
     8281 caggaagagg aagacctgtt cgcccccgtc ttggattccg tcaaaaaccg ctggatgtgg
     8341 ctcgccgtca acctctgcac cgccttcctc gccagccgcg tcatcggcgc gtttgaaggc
     8401 agtatcgaaa aaatcgtcgc actcgccgcg ctgatgccca tcgtcgccgg catcggcggt
     8461 aactcgggca accagacgat taccatgatt gtccgcgcga tggcgatggg gcagctgacg
     8521 gatatgcagg cgggaaggct gttgaaaaaa gaagtcggcg tcgccttggt caacggcatt
     8581 atttggggga cggtgatggg agcggtttcg tggctgcttt acggcagcct cggcatcggt
     8641 ttggtcatgg ttgccgcgat gacgctcaac ctcctgctgg cggcaacggt cggcgtattg
     8701 attcccgtgg taatggaaaa attcggacgc gatcccgcgc tgggcagctc ggtgctgatt
     8761 accgccgtta ccgactccgg cggcttcctc attttcttga gtctcgccac cctgttcctg
     8821 ctttaaatgc cgtcccgacc cgcgcaaaaa tgccgtctga agcagaagct gcttcagacg
     8881 gcattcgacc attcatcctt gttgctcaag attattggac ggtatgtcgg ggcagccctt
     8941 tggcaacgcc gaccacatcc tccccgaaca gcgcgttgac atcggtttca tcaaacacgt
     9001 atttgctgtg gcagaagtcg caatcgactt cgatgctgcc ttgttccgcc accacgccgc
     9061 cgacttcttc cccgcccagc atcaacagca tatcgctgac cttgccccga gaacaggtac
     9121 atgaggattc aaacgtttcc ggctcgaaca cgcgcggcgg cgtttcgtgg aacaggcggt
     9181 ataaaacgtg ttgcgcgtcc aattctgcca gctcctccgc cgtcagcgtg cgcgccagcg
     9241 tactgacgtg ttcccatgcc tcttcatcca acacctcttc aggcagacgc tgcaccagca
     9301 gcccgcccgc cgcttcatcg cttgcagaca gggcgatgtg cgtatcaagc tgttcggaac
     9361 gtttcatata gttcaccaac atttgcgcga tgctgccgcc ttccaaaggc actacgccct
     9421 gccagggttc gccgtctttg ggttgcagcg tcaatacgaa cacgccgttg ccgcccaaaa
     9481 ggtcgccgag gctttcgtca tcggctattt ctgcggtttc gtcccaccgc gcggttgcac
     9541 ggacggtacg gtcggaagtc gcttccgcaa ccagcatttt cagttttccc tgcccctgaa
     9601 cctgcacaat cagcgtgcct tcgtttttaa ggttgcccga cagcagaacg ccggcagcca
     9661 gtagctctcc caaggcgcag cggatggcgg cgggatagtt tttctgtttt acaatgtgct
     9721 gccacacgtt ttccagacgg acgtgcagcc cgcgcacggg catatcgtcg aagataaagc
     9781 gggtacgcgc atcggcgcgg ttgatggcgg tttgattcat gattttctct gactgattgt
     9841 tcggatggcg gctatatggt tgcggtcggc gcgaaaacaa ggcggacggc ggatgcgctt
     9901 cccaaattct caataaatta tataaaaatc aacatattaa ctcaatctaa caagccgttt
     9961 tttgccaaac agccgttttt ttatatacaa tcaacaagat attttcgact gatacagcat
    10021 aacatcgcac ggcggcacga tgcctcctgc gcggaaacac cgatatggat tcttttttca
    10081 aaccggcagt ttgggcgatt ttgtggctga tgtttgccgt ccgccccgcc cttgccgacg
    10141 agttgaccaa cctgctcagc agccgcgagc agattctcag acagtttgcc gaagacgaac
    10201 agcccgtttt acccgtcaac cgagcccccg cccggcgggc gggcaatgcc gacgaactca
    10261 tcggcagcgc gatggggctt ttgggtattg cctaccgcta cggcggcaca tcggtttcta
    10321 ccggttttga ctgcagcgga ttcatgcagc acatcttcaa acgcgccatg ggcatcaacc
    10381 tgccgcgcac gtcggcggaa caggcgcgga tgggcgcacc cgttgcccga agcgaattgc
    10441 agcccgggga tatggtgttt ttccgcacgc tcggcggcag ccgcatttcc catgtcggac
    10501 tttatatcgg caacaaccgc ttcatccacg cgccgcgcac ggggaaaaat atcgaaatca
    10561 ccagcctgag ccacaaatat tggagcggca aatatgcgtt cgcccgccgg atcaagaaaa
    10621 acgacccgtc acgctttctg aactgatttc ccaaggaata cgcaatgagt atgcccgaaa
    10681 tgcccaaatg gtacggcgat gacggacaga tcgtatcctg taccgaaaag gtcaaagtga
    10741 tgtccgaaaa tatggccgag ctgtatcaga cggcacaaga cgcgtttgaa gacgcgctgc
    10801 tgatgggttg cggcgaacgg cagttgcgcg cttacctgct cgcgctgatt gaaggtttgg
    10861 aaaatcccta ccgcaaagtc tgaacacgcc ccggttgctg cggcacggtt tatccgtgcc
    10921 gtttttgcgt ttgtgcgcgg cttcggcttt tcagacggca tatttgacgt tatgattaaa
    10981 cagttaacaa gatttatcac aacgccgtca aaagacagac acacaacatg aacatcatcc
    11041 gcgcgctcct catcatcctc ggctgcctcg ccgccggcga aaccgccgtt ttcctagcag
    11101 gcatcaaact gcccggcagc atcgtcggca tgggcgtgct gtttgcgctt ttgcaggcgg
    11161 gttgggtcaa aacgtcttgg ctgcaacagc ttaccgacgc gctgatggca aacctgacgc
    11221 tgttcctcgt gccgccctgc gtggcggtca tcagctattt ggatttgatt gccgacgatt
    11281 ggttttcgat actggtttcc gcctccgcca gcactttgtg cgtactgctg gttacgggca
    11341 aggttcaccg ctggatacgg agcattatct gatgagcgaa atcctcaggc agcccggcat
    11401 cctgcttttc ctcacgcttg ccgtgtacgc gcttgcgatt atcgtgcgca cgcgcacggg
    11461 caatatcttc tgcaaccccg tactcgtcag cactatcgtg ctgattgcct acctgaaaat
    11521 cctcggtatc gattatgcgg tgtaccacaa cgccgcgcaa ttcattgatt ttcggctgaa
    11581 acccgccgtc gtcgtgcttg ccgtgccgct ctaccaaaac cgccgtaaaa tcttcaacca
    11641 gtggctgccc gtcatcgttt cgcagcttgc gggcagcgtt acgggcattg ttacggggat
    11701 gtattttgcc aagtggctgg gcgcggaacg cgaagtcgtc ctcccgctcg cgtccaaatc
    11761 tgttaccaac cccatcgcca tcgaaatcac cagctccatc ggcggcattc ccgccattac
    11821 cgccgccacc gtcatcattg ccggtctggt cggacagatt gccggttaca aaatgttgaa
    11881 gaacacggtc gtcatgccct cgtccgtggg tatgtcgctc ggcacggctt cgcacgcgat
    11941 gggcattgcc gcctcgctcg aacgcagccg ccgcatggcg gcatacgcgg ggctggggct
    12001 gacgttcaac ggcgtgctga ccgcgctgat tgcgccgctg cttatccccg ttttggggtt
    12061 ctgaacccgt ttcagacggc atttcaaccc atgccgtctg aacgccgaca cactcgcaag
    12121 gagaaccgtt atggctgtca acctgaccga aaaaaccgcc gaacaactgc ccgacatcga
    12181 cggcattgcc ctctacaccg cccaagcagg cgtgaagaag cccgggcata ccgacctgac
    12241 actgattgcc gtagccgccg gcagcaccgt cggtgcagtc ttcacgacca accgtttctg
    12301 tgccgcgccc gtccacatcg ccaaatcgca ccttttcgac gaagacggcg tgcgcgccct
    12361 cgtcatcaac acgggcaacg ccaacgcggg tacgggcgca cagggcagaa tcgatgcttt
    12421 ggcagtgtgt gccgccgccg cccggcaaat cggctgcaaa ccgaaccagg tgctgccctt
    12481 ctccaccggc gtgattctcg aaccgctgcc cgcagacaaa atcatcgccg ccctgcccaa
    12541 aatgcagcct gccttctgga acgaagcggc acgcgccatc atgaccaccg acaccgttcc
    12601 caaagccgcc tcgcgcgaag gcaaggtcgg cgaccaacac accgtccgcg ccacaggcat
    12661 tgccaaaggc tcgggcatga ttcatcccaa tatggcgacc atgctcggtt tcatcgccac
    12721 cgatgccaaa gtttcccaac ccgtcctcca actgatgacg caggaaatcg ccgacgaaac
    12781 cttcaacacc atcaccgttg acggcgacac cagcaccaac gacagcttcg tcatcatcgc
    12841 caccggcaaa aacagccaaa gcgaaatcga caacatcgcc gacccgcgtt acgcccaact
    12901 caaagaattg ttgtgcagcc ttgcgctcga actcgcccaa gccatcgtcc gcgacggcga
    12961 gggcgcgacc aagttcatca ccgtccgcgt cgaaaacgcc aaaacctgcg acgaagcccg
    13021 ccaagccgcc tacgccgcgg cacgttcgcc actggtcaaa accgcctttt tcgcctccga
    13081 ccccaacctc ggcaggctgc tcgccgccat cggttatgcc gacgttgccg acctcgatac
    13141 cgacctcgtg gaaatgtatc tcgacgatat tttggttgcc gaacacggcg gacgcgccgc
    13201 aagctacacc gaagcacaag ggcaggcggt gatgtcgaag gacgaaatca ccgtccgcat
    13261 caagctgcat cgcggacaag ccgccgccac cgtctatacc tgcgacctgt cgcacggata
    13321 cgtttccatc aacgccgact accgttcctg acccgacacg gcttcagacg gcatacataa
    13381 aatgccgtct gaaccgtcgg acaacatacc atgacctcca cattcccccg ccgcctcgcc
    13441 cgcaaaatcc gccaaacccg ccgcctgtcg cgcaaaagca tcgcctttct gttccttctg
    13501 gcaggttcgg cactcgtcgc cctgaccgca ctgttttttg cccatcttgc cgattttgcg
    13561 ctggaactga acgccaaact ggttcaacaa tacccgtggt tcgcatggat tgccctgccg
    13621 ccgggactgc ccgtcatcgt ctggctcaca cgcaaattcg cccccttcac cgccggcagc
    13681 ggcatccctc aagtcatcgc ctcactgtcg ctgccctacg gcgcacaaaa aacgcggctg
    13741 atacgcctcg ggcaaacgct gctgaagatt ccgctgacct ttttgggtat gctgttcggc
    13801 gcgtccatcg gacgcgaagg cccgtccgtg caggtcggcg cggcagtaat gggcgcgtgg
    13861 ggcgcgtggt gcaaaaaaca cggtttggcg tttaagggta tgcaggaaaa cgatttgatg
    13921 gcggcgggcg cggcgggcgg tttggcagca gcgttcaacg cgccgctggc gggcgtgatt
    13981 ttcgccattg aggaactggg gcgcggcatc atgctgcgct gggagcggca gattctgctc
    14041 ggcgtactcg ccgccggttt catacaggtc gccattcagg gcaacaaccc gtatttttcg
    14101 ggtttcaacg gcggcgtttt ggaaaatatt ttgatgtgga tcgtggcggc ggggctggtt
    14161 tgcggcgcgg ctggcggcat atttgcccgt atgctccatc gcggtgcggc ggcgtttgca
    14221 ccgcgcaaga tacgcggctt catccgcaac cgtccgctgc tgctggcggc actgatgggg
    14281 ctgctgctcg ccctgctcgg cacgttctac caaggcaaaa cctacggcac cggctaccac
    14341 gaagccgccc aagccctgca cggcatctac gaagcccccg tcggactcgc cgccgccaaa
    14401 tggctcgcca ccgtattcag ctattgggca ggcatcccgg gcggcatttt cactccctcg
    14461 ctgaccatag gtgcggtttt gggcgagcat atcgccgcca tcgccgacat atcgcagggt
    14521 gcaaacatca tcgtcctcat ctgcatggcg gcatttttag cgggcgcgac acaatcgccg
    14581 attacttccg ccgtcgtcgt catggaaatg accggcgggc aaagcctgct gttttggatg
    14641 ctgattgcct gcattttcgc ctcgcaggtt tcgcgccagt tttcgccgcg tccgttctac
    14701 cacgcatcgg gaatgcgctt ccgccggcgc gtgcttcagg aaaccgccgc ccaaaccggc
    14761 aatgcgcccg caagaccgca agccgcaaac agcaaaacgg gaatgccgtc tgaaaattaa
    14821 aacgcccccg atcaaacgcc ggcagccgcc ttgattcaaa taccgttccg ctgcgcttga
    14881 aatttcagca acaatgccgt ctgaacgaca gaatgcggtt ttcagacggc atttccccat
    14941 cccgatattg ttcaaacaaa accgaagcgt ttgctataat tgcacttttt accgcacacg
    15001 cacgcccatg tttcccgatt tctcccaaac cctctccaaa gaccgccact tcctgcaatc
    15061 cgccttcaaa aatcccaaca aatacggcgg cttgtccaaa atcgaagaaa aataccgaaa
    15121 atcgcacgaa atcttcttga aacgtttggc cgcattgcca aaacccgaat tcgacgacac
    15181 cctgcccgtt cacgaaaaac tcgaagaaat caaaaaaacc gttgccgaaa atcaggtaac
    15241 gattatctgc ggcgaaaccg gttcgggcaa aaccacgcag ttgcccaaga tttgtttgga
    15301 actcgggcgt ggggcggcag gattgatcgg gcatacccag ccgcgccgtt tggccgcgcg
    15361 ttcggtggcg gagcggattg ccgaagagct gaaatccgaa atcggcagcg cggtcggcta
    15421 taaagtgcgc tttaccgacc acacctcgcg cgatgcctgc gtcaagctga tgaccgacgg
    15481 catcctgctg gcggaaaccc agaccgaccg ttatctcgcc gcctacgaca cgattatcat
    15541 tgacgaagcg catgaacgca gcctgaacat cgacttcctc ttgggctacc taaaacaact
    15601 cctgccgcgc cgccccgatt tgaaagtcat catcacatca gccaccatag acgcagaacg
    15661 cttctcccga cacttcaacg gcgcgcccgt actggaagtg agcgggcgca cctatcccgt
    15721 cgaaatcctc taccgaccgc tgaccggcaa agacgaagac gacgcagaag tcgagctgac
    15781 cgacgcgatt gtcgatgccg ccgacgaatt ggcacgctac ggcgaaggcg atattttagt
    15841 attcctgccg ggcgaacgcg aaatccgcga agcggcagaa gccctgcgca aatccacact
    15901 gcgccgcaat gacgaaatcc tgcccctgtt tgcacgcctg tcgcacgccg aacagcacaa
    15961 aatcttccac ccttcaggcg caaaatgccg catcgtgctg gcgaccaacg tcgccgaaac
    16021 ctcgctcacc gtgccgggca tcaaatacgt catcgacacc ggcctcgcgc gcgtcaaacg
    16081 ctattccgcg cgggcaaaag tggaacaact tcacgtagaa aaaatctccc aagccgccgc
    16141 ccgccaacgc tccggccgct gcggacgcgt ctcagcaggc gtgtgtatcc gactgttttc
    16201 agaagaagat tttaacagcc gtcccgaatt taccgatccc gaaatcgtcc gcagcaacct
    16261 cgccgccgtc atcctgcgca tggcatcgct gaacttgggc gacgtagcag cattcccatt
    16321 tttagaaatg cccgattcgc gatatatcaa tgacggtttt caggtgttgc tggaattggg
    16381 ggcagtggag gagaattaga ttgaaagcag gctacctgaa aatgtgaagc agcctgcact
    16441 tttaaggtta aattgaataa agttcagatg acttaaacgt agaaaaccaa gcggtcagct
    16501 aactgacggc ttggtttttg acccacccct gaccctaatt cttacaacgg tgtttcaggt
    16561 attcagtttc cctttctcct agctgtattc acagcaacag ttgtaagccg agattttgat
    16621 ttacctttgc gctatgaatg caaacaggca tttcataggg cttaatcaat aatcatgatg
    16681 ataaatcgtc aggcttaata ttgcccaatg aatagcattt tatttaaggc tgctttcctg
    16741 ccttgggggt ttaaggtatt ttaccatgaa gcctgatatt caataatatc gtttacagag
    16801 aatccatgat ttaacaaggc ttgccgaata agaaaaggta aagattgagt atatttaggg
    16861 aagttacaac aaaattccaa ccaaaacctg cccatatttt caatatcttt actacttaat
    16921 ttatcaaaaa atcgaagtaa ctcgctaatt cctattgatt gatttaaaat agtttgccat
    16981 gctgctgctt ccaaactttt atcataatcg tcataaattt tcttttttaa aatcatataa
    17041 aattgttcat tcccttgata atgaatgata aatttatatg ctggtacaaa tttattatag
    17101 ccattgtttt taccaataag aagactaaaa attatttttc cttctacttt aataaattca
    17161 attttagatg tctctgcatt tttgcgccaa tacagcttga atgtgttcct acgtaatttg
    17221 tatccgaata aatccaataa aggtttgagc tgttgctctg ttcccaattt ttcgcccttg
    17281 ctccatgcac tcacaactga ttgttgtgtg cggcaagttt tagcaatttc agtttgcgtc
    17341 caaccattat tcaatgccag cctaacgagt tctctcgtct gcttaaatgg ttttttcttt
    17401 gtcttttcag attgtgtttc tgtcttttcg gtttgtttgt tttccataat acccccaata
    17461 tgccaattaa aatggcgata ccaagtataa tatcgccatt tataggcaag gcaatggcta
    17521 ttttttgcct ttaccgctat tcgcaggatt ttgtggattc atttgccagc cacgatttcc
    17581 ctgattttgt gaatacgttt tatttgttcc tgacgtgcct ttattagcat tgctcatgtt
    17641 tgattgatga ttggcttgtt tattgctcat tttagttcta ctcctgaaaa agatatttaa
    17701 tctgcttggt gaaactgtgg attgctttgc aatcgctcgg tgttgatggg attgcaattc
    17761 tacggttatt tggtgctacc aaaatattgt gcttttttct attttgtatt ttccaccccc
    17821 tatttagtag tgaatgaata aaagaattga tttttgaatt ggtgctaaat tttttcatga
    17881 ttacccccca tgacgatagg tagcatcata aaaaattcgt agcaaaagtt ccattgataa
    17941 aaagttgata aattttgata ttttatgata ttttgtaatt tattgatatt taatgatttt
    18001 aagatagaca ttctatggat ttaagattta cttctcgttt tttgaaaaac aatctgtaaa
    18061 cagatagctg ctgaagaata ccgttgccga gccccaaaac ccgtcctgca acttttattg
    18121 tgaactgccc attatgaaaa aatccctttt cgttctcttt ctgtattcat ccctacttac
    18181 cgccagcgaa atcgcctatc gctttgtatt cggaattgaa accttaccgg ctgcaaaaat
    18241 ggcggaaacg tttgcgctga catttatgat tgctgcgctg tatctgtttg cgcgttataa
    18301 ggcttcgcgg ctgctgattg cggtgttttt cgcgttcagc atgattgcca acaatgtgca
    18361 ttacgcggtt tatcaaagct ggatgacggg tattaactat tggctgatgc tgaaagaggt
    18421 taccgaagtc ggcagcgcgg gcgcgtcgat gttggataaa ttgtggctgc ctgctttgtg
    18481 gggcgtggcg gaagtcgtgc tgttttgcag ccttgccaag ttccgccgta agacgcattt
    18541 ttctgccgat atcctgtttg ccttcctaat gctgatgatt ttcgtgcgtt cgttcgacac
    18601 gaaacaagag cacggtattt cgcccaaacc gacatacagc cgcatcaaag ccaattattt
    18661 cagcttcggt tattttgtcg ggcgcgtgtt gccgtatcag ttgtttgatt taagcaagat
    18721 ccctgtgttc aaacagcctg ctccaagcaa aatcgggcaa ggcagtattc aaaatatcgt
    18781 cctgattatg ggcgaaagcg aaagcgcggc gcatttgaaa ttgtttggtt acgggcgcga
    18841 aacttcgccg tttttaaccc ggctgtcgca agccgatttt aagccgattg tgaaacaaag
    18901 ttattccgca ggctttatga cggcagtatc cctgcccagt ttctttaacg tcataccgca
    18961 cgccaacggc ttggaacaaa tcagcggcgg cgataccaat atgttccgcc tcgccaaaga
    19021 gcagggctat gaaacgtatt tttacagtgc ccaggctgaa aaccaaatgg caattttgaa
    19081 cttaatcggt aagaaatgga tagaccatct gattcagccg acgcaacttg gctacggcaa
    19141 cggcgacaat atgcccgatg agaagctgct gccgttgttc gacaaaatca atttgcagca
    19201 gggcaggcat tttatcgtgt tgcaccaacg cggttcgcac gccccatacg gcgcattgtt
    19261 gcagcctcaa gataaagtat tcggcgaagc cgatattgcg gataagtacg acaacaccat
    19321 ccacaaaacc gaccaaatga ttcaaaccgt attcgagcag ctgcaaaagc agcctgacgg
    19381 caactggctg tttgcctata cctccgatca tgaccagtat gtgcgccaag atatctacaa
    19441 tcaaggcacg gtgcagcccg acagctatat tgtgcctctg gttttgtaca gcccggataa
    19501 ggccgtgcaa caggctgcca accaggcttt tgcgccttgc gagattgcct tccatcagca
    19561 gctttcaacg ttcctgattc acacgttggg ctacgatatg ccggtttcag gttgtcgcga
    19621 aggctcggta acaggcaacc tgattacggg cgatgcaggc agcttgaaca ttcgcaacgg
    19681 caaggcggaa tatgtttatc cgcaataagc agcgtgaaaa ccaataaaga caaatttaga
    19741 tgatgtcggg gaagatgccc gaccgacaag actatgcaaa atatgaaaaa ccaagtacgc
    19801 ggatcaggca tggatgcccg atccaacccg gccaatgttt cagacggcct gcaaaacagt
    19861 tcgggccata tcggtaccaa cacgcgctac cgcctgacca agctcggcga acaaatggcg
    19921 cgcctgccca tcgatccgaa aattgcgcgc attttgctgg cggcgaagaa acacgactgc
    19981 atggcggaaa tattggtgat tgcgtctgcg ctgtcgattc aagacccgcg cgaacggccg
    20041 ctggaagcgc gcgatgccgc ggccaaggcg catgaacgct ttaccgacaa gcaatccgat
    20101 ttccttacct acctgaatat ttgggacagt ttccagcgcg agcgcgacaa aggtttgtcc
    20161 aacaagcagt tggtgcaatg gtgccgtcaa tattttctgt cgcacctgag gatgcgcgag
    20221 tggcgcgagc tgcaccacca gcttgcccaa accgcgattg aaatgggctt gaccaccaaa
    20281 gaagctgcgt ttagacgacc tcccgaagtc aagcaactga cgtcgtctga gaatgcgggc
    20341 gaccaagacc tatctgccaa actcaaacaa aaacaactgg ataagaaaca acaccgcacc
    20401 caaatccgcg ctaccaaaga agcgggctac gaacaaatcc accgcgccct gctcaccggc
    20461 ctcatcgcca acgtcggcat gaaatcgccc gacagcaacg actacaccgg cgcgcgcggc
    20521 agccgcttcc accttttccc cgcctccgcc ctgttcaaag ccaagcccaa atgggtgatg
    20581 gcggcagaat tggttgaaac cacgcgcctt tacgcgcgcg acgtcgccgt tatccagccc
    20641 gaatggatcg agcaggaagc cccgcacctt gtccgctacc attatttcga gccgcactgg
    20701 gaacaaaaac gcggcgaagt cgtcgccagc gaacgcgtaa cgctttacgg cctggccgta
    20761 ttgccgcgcc gccccgtgtc ttacggtaaa gttgcgcccg aagaagcacg tgaaatcttt
    20821 atccgcagcg cgttggtggc gcaggaatac gatttgaaag cggatttttt tgtccacaac
    20881 aaaaagctga ttaaagaaat taccgaactc gaacacaaat cgcgcaagca agacgtattg
    20941 gtcgatgacg aagccctgtt tgcgttttat cacgaacggc tgcccgattt ttatatggcg
    21001 gattcggttt cagagggcct gtgtcctgca aatccgcagc aaaccacccc ctcccccgtg
    21061 ggggaaggct ggggagaggg caaaacagtt gccgcacaaa ccaacttttc cgcaacctca
    21121 gcaagccctc tccctaaccc tctcccacag gagagggaac agagtgcctc agtttcaacg
    21181 gtttcaggca gtctgaaaac aatgtcttgc gaagcaaggc tgaatttctg cgaagctaaa
    21241 actaaaaccg aaagcagcct gcactcccaa aggctatctg aaaactacac cccgccattt
    21301 tcagacggcc tgcgtcctgc aaacccccag caaaccaccc cctcccccgt gggggagggc
    21361 tggggagagg gcaaaacagt tgccgcacaa accaactttt ccgcaaccgc agcaaaccct
    21421 ctcccacagg agagggaaca gagtgcctcg gctttaacag tttcagatga ccccaaaccc
    21481 aaaaagcagc ctgcatctca aaaaggccgt ctgaaacctt tgccccttgc cgatatccgc
    21541 acctttgaag cctggctcaa aaccgccgag cgcgacaacc cgcgcctgtt gttcctcagc
    21601 cgcgacgacc tcatgcagca cgccgccgcg cacatcaccg aagaacaatt ccccaaacac
    21661 tggcagaccg cagacggcaa attcaaactc agctaccgct tcgagccgca ccacccgctc
    21721 gacggcgtta ccctcaccct gccgctcacc gtgctcaacc gcatcagccc cgccgccctc
    21781 gaatggctcg tgcccggcat gatacgcgaa aaaatccagc tacaaatcaa agcactgccc
    21841 aaacaaatcc gccgcatctg cgtacccgta cccgaattca tcacccaatt tttaagccaa
    21901 aaccccgacc gcaacgcccc catcctgccc cagctcgccc aagccatcgc caaaaccgca
    21961 ggcgacatcc gcatactcga gcaaatcaac caagacgaat gggccgcgtt caggctgccc
    22021 gaacactgct atttcaacct ccgcatcatc gacgacggcg gacaagagtt agccatgggt
    22081 cgcgatttaa tccaaatcca acaacaactt ggcaaagccg ccaccaccac cttccgcgac
    22141 aacacccaag aattcgagcg tgacaacgtt accgcatggg acatcggcat cctgcccgaa
    22201 tccatcaaat tcgcccgcgg caaacaacag ctcaccggct acctcggcct gcaaaaagaa
    22261 aaagacggcc gcatcgccct gcgcctgttc gacacgtctg ccgccgccgg acacgcccac
    22321 agattaggcg tgattgaact catgaaacta caactaaaag aacaagttaa agatctgaac
    22381 aaaggcatcc aaggcttcac ccaagccgcc atgctgctca aacacatcaa cgccgacacc
    22441 ctgcgcgacg acctcaccca agccgtctgc gaccgcgcct ttatcggcga agacaagctg
    22501 ccgcgcaacg aaaaagcctt caaagaacaa atcaaacgcg cccgcagccg cctgcccgcc
    22561 gtcaaagaag ccctcagccg ctacctgcag gaaaccgcag ccgcctacgc cgaactgaac
    22621 ggcaaactcg gcaaacaccc attgacccac ctcctaagac tacgcctgca aaccctgctt
    22681 gcacccggct tcgccacccg caccccgtgg gcacaatggc cgcgcctccc catctacctc
    22741 aaagccatga ccctgcgcct cgaaaaatac agcagcaacc ccgcccgcga cgcagcccgc
    22801 gaagccgata tccaagagct ggaacaaatg tggcaggaaa aaactgacgg cttggcgaaa
    22861 caaggacagc ccgtttcaga cggcctcgcc gcgtttaaat ggatgattga agaattgaga
    22921 gtgtcgctgt tcgcgcagga gttgaagacg ccgtatccgg tgtcggtaaa gcggctgttg
    22981 aagatgtg
//
LOCUS       DAXDSD010000022        22912 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXDSD010000022 DAXDSD010000000
VERSION     DAXDSD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22912
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.022"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(142..1530)
                     /locus_tag="ACFB2Q_000676"
     CDS             complement(142..1530)
                     /locus_tag="ACFB2Q_000676"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC0917751.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPARKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            1858..2805
                     /locus_tag="ACFB2Q_000677"
     CDS             1858..2805
                     /locus_tag="ACFB2Q_000677"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC0917752.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPGEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="ACFB2Q_000678"
     CDS             complement(2857..3189)
                     /gene="trxA"
                     /locus_tag="ACFB2Q_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC0917753.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            3487..3960
                     /gene="dnrN"
                     /locus_tag="ACFB2Q_000679"
     CDS             3487..3960
                     /gene="dnrN"
                     /locus_tag="ACFB2Q_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFC0917754.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            complement(4031..4852)
                     /gene="nadE"
                     /locus_tag="ACFB2Q_000680"
     CDS             complement(4031..4852)
                     /gene="nadE"
                     /locus_tag="ACFB2Q_000680"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC0917755.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHQTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            5049..6404
                     /gene="xseA"
                     /locus_tag="ACFB2Q_000681"
     CDS             5049..6404
                     /gene="xseA"
                     /locus_tag="ACFB2Q_000681"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC0917756.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
     gene            6697..8238
                     /locus_tag="ACFB2Q_000682"
     CDS             6697..8238
                     /locus_tag="ACFB2Q_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC0917757.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYVGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIVGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(8297..9346)
                     /locus_tag="ACFB2Q_000683"
     CDS             complement(8297..9346)
                     /locus_tag="ACFB2Q_000683"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC0917758.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRSLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(9355..9507)
                     /locus_tag="ACFB2Q_000684"
     CDS             complement(9355..9507)
                     /locus_tag="ACFB2Q_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917759.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            9567..10199
                     /gene="pdxH"
                     /locus_tag="ACFB2Q_000685"
     CDS             9567..10199
                     /gene="pdxH"
                     /locus_tag="ACFB2Q_000685"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC0917760.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(10329..11339)
                     /locus_tag="ACFB2Q_000686"
     CDS             complement(10329..11339)
                     /locus_tag="ACFB2Q_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC0917761.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELASGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDININIPGCKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILPATDTGYAP
                     IRSILLDLIRQNSSRAAHFYWGARHQDDLYALEEAQGLACRLKNTCFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(11431..12861)
                     /gene="gatB"
                     /locus_tag="ACFB2Q_000687"
     CDS             complement(11431..12861)
                     /gene="gatB"
                     /locus_tag="ACFB2Q_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC0917762.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGGDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRIDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            complement(12904..13836)
                     /locus_tag="ACFB2Q_000688"
     CDS             complement(12904..13836)
                     /locus_tag="ACFB2Q_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC0917763.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDVLC
                     QMEDCRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDDMVTKMKDSLKTEARGISDGIQTEKPLRRTV"
     gene            complement(13833..15278)
                     /gene="gatA"
                     /locus_tag="ACFB2Q_000689"
     CDS             complement(13833..15278)
                     /gene="gatA"
                     /locus_tag="ACFB2Q_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC0917764.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFVSPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSFEREKEDYTRDLDKPLKGVKIGLPKEYFSEGNSTDVQTA
                     LQNTIDLLKAQGAEPVEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADTSPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            complement(15341..15631)
                     /gene="gatC"
                     /locus_tag="ACFB2Q_000690"
     CDS             complement(15341..15631)
                     /gene="gatC"
                     /locus_tag="ACFB2Q_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC0917765.1"
                     /translation="MALTLADVDKIARLSRLQLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEVALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            15781..16434
                     /locus_tag="ACFB2Q_000691"
     CDS             15781..16434
                     /locus_tag="ACFB2Q_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC0917766.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            16439..17782
                     /locus_tag="ACFB2Q_000692"
     CDS             16439..17782
                     /locus_tag="ACFB2Q_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC0917767.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(17831..18259)
                     /locus_tag="ACFB2Q_000693"
     CDS             complement(17831..18259)
                     /locus_tag="ACFB2Q_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC0917768.1"
                     /translation="MNFALSVITFTLASFLPVPPAGTAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(18605..19861)
                     /locus_tag="ACFB2Q_000694"
     CDS             complement(18605..19861)
                     /locus_tag="ACFB2Q_000694"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC0917769.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(19897..20766)
                     /locus_tag="ACFB2Q_000695"
     CDS             complement(19897..20766)
                     /locus_tag="ACFB2Q_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC0917770.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            complement(20807..21622)
                     /locus_tag="ACFB2Q_000696"
     CDS             complement(20807..21622)
                     /locus_tag="ACFB2Q_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC0917771.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            21866..22651
                     /locus_tag="ACFB2Q_000697"
     CDS             21866..22651
                     /locus_tag="ACFB2Q_000697"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC0917772.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGDRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT         4642 a         5814 c         6751 g         5705 t
ORIGIN      
        1 gtaccggttt aaatttaatc cactatattg cagattaatc ctttgggtat caaagccggg
       61 cggtgctgaa acattacaaa aatgccgtct gaaactcgaa atcggtttca gacggcattg
      121 gtttacgcgg caggttgggg cttagcggtc tttgtaacgg cgttcgcttt tgcctgcaaa
      181 agagtcgccg gctttgcgtt tgcctttgaa gccttcgccg gtttttttga aactgtcttt
      241 cttaaagcct tctttcttga aaccttcgcc gcgcgttttg ccgccgaagc cttctttgcc
      301 cggtttatga tcgccgcgcc ggccgccgga tttcctatcg ccccagccgc ctttgccttt
      361 cggcttgccg cctgcggatt tgcgtttgcg ggccggctcc atgccttcga tggtcagttc
      421 gggcagtttg cggttaatgt atttttcgat tttgtggact ttgacgtatt cgttcacttc
      481 ggcaaacgta atcgcaatac ccgtgcggcc tgcgcggccg gtgcgcccga tgcggtggac
      541 gtagtcttcc gcctgtttcg gcaggtcgta gtttatgacg tgggtaatgg tcggtacgtc
      601 aataccgcgt gcggcaacgt cggtggcaac caaaattttg cagcggcctt tacgcaaatc
      661 cgtcagcgtg cggttgcgcc agccctgcgg catatcgccg tgcaggcagt tggcggcgaa
      721 acctttttcg tacaattcat ccgcgatgac ttcggtcatc gctttggtgg acgtgaaaat
      781 cacacattgg tcgatgttgg catcgcgcag gatgtggtcg agcaggcggt ttttgtggcg
      841 catatcgtcg cagtacaaca actgctcttc gattttgcct tggccgtcca cgcgttcgac
      901 ttcgataatt tcagagtctt tggtcagttt gcgcgccagt ttgccgactg cgccgtccca
      961 agtggcggag aacaataaag tctgacggtc ggccggggtg gcttccacga tggtttcgat
     1021 gtcgtcgata aagcccatat ccaacatacg gtcggcttcg tccaaaatca gcacttccaa
     1081 acgttcaaaa tcaactttgc cgctttgcat caggtccgtc agacggcccg gagtggcgac
     1141 aatcaaatcg accggtttgc tcagggcgcg ggtttggtag ccgaacgatg tgccgccgac
     1201 gatgctgacg gtgcggaacc agcgcatatt tttggcatac gccagcgcgt ttttctcgac
     1261 ttgcgccgcc agttcgcggg tcggtgccaa caccagcgcg cgcgggcctt tgcccggttt
     1321 ttcgctgcgt ttggtcagtc gctgcaaagt cggtaacaga aaggctgcgg tttttccgga
     1381 gccggtttgc gccgaagcca tgatgtcgca gccttccaaa gcaaacggaa tcgcctgcgc
     1441 ctgaatcggc gtcgggcttt cgtaaccctc gctgctgacg gcggacaaaa tgtttttatc
     1501 aaggttcaaa tcggcaaatt taatagacat ggctatcctt ggaagacaac aacgcccgcg
     1561 tccgaaaggt tcagacggcc tgaagcaggg aagggtaacg atacggggaa tgtgaagtta
     1621 cagggttgtc gcaagcggct tgcttgtggt ttacatcgac ggcaacctgc acagatatgc
     1681 tttgcaaaat caaagcaaca gaagaaacgc gcccgtaagg gcggtttagg ttggaaggcg
     1741 atggcttcgt atgacaagat aagcgacgga tggtataggg tttgcccgaa aaggtcaagc
     1801 aatcgggcgt taattacagt aaaatttccg ttttccactt tattccgcct gaaatcaatg
     1861 accgacatca caccgtttgc caaccgcttg ggcaaaaaca tcaaacacct tatgaaatgg
     1921 gcgaaacgca acggcatcga agcctggcgc atttacgacc gcgacatccc ccaattccct
     1981 tttgccgccg atgtttacgg cgatcgaatc caccttcagg aatacgatac cggctggctg
     2041 atgcggcccg gagaatacga agcgtggctt gccgaagtat tggaagccgt cgctttcgtt
     2101 accggttttg aacccgaaca aatccgtctc aaacgccgcg aacgccaaaa aggtttgcag
     2161 cagtacgaga aaaccggcaa ggcgggcgac gatttcgtca ttgccgaaaa cggccgcaag
     2221 ttttgggtca acctcgacaa atacctcgac accggcttct tcctcgatca ccgcaacacg
     2281 cgcaaaaaag tcggcgaaac ggcggcgggc aaacgttttt tgaacctgtt ttcctacacc
     2341 ggcagcttca ccgtctatgc cgccaccggc ggcgcggcat ccagcgaaac cgtcgatttg
     2401 tccaacactt atctcgattg ggcgaggcgc aattttgaat tgaacggcat cgatatggaa
     2461 cggcacaaaa tcgtccgcgc cgacgtgttc caataccttc agacggcata tggggaaggc
     2521 aggcagttcg acctcattgt gatggacccg cccagttttt ccaacagcaa gaaaatgccc
     2581 ggcatcctcg acatccagcg cgaccatcaa aaactcatag acggcgcggt gaacctgctc
     2641 gcttcagacg gcattttgta tttttccaac aacctgcgca gcttcgtttt ggacgattcg
     2701 gtatcggaac aatacgccgt gaaagacatt tccaaacaat ccgttcccga agatttccgc
     2761 aacaaaaaaa tccaccggtg ttgggaaatc cgccacaaat cttaagggtg cgaaaaagcc
     2821 gccggaatct tccggcggct tttcgattgc gctttatcag gcgatggagg cttcgacaaa
     2881 ggcggtcagc tgaccttttg ccaatgcgcc gactttggtg gcgacgactt cgccgttttt
     2941 gaacaccatc agggtcggaa tgccgcgcac gccgaaacgg gacggggtgg cttcgttgtc
     3001 gtcgatgttg attttgacta ctttcagacg gccttcaaat tcggcggcaa tgtcgtccaa
     3061 aatcggggca atcattttgc aggggccgca ccacggagcc caaaagtcca gcaggacggg
     3121 gaggtcggat ttcaaaacat cttgctcgaa ggcggcatcg ccggtgtgta cgattaattc
     3181 gctgctcatt atttttcctt ttcggttggg attcgcacga tagcgtgcag gatagggctt
     3241 gggcataaaa atttcaagtg ggcgggcggg ataatagttt ttctgtaccg ccccaatcgc
     3301 ctttgcaaat tctaaaaaac ggttcggaca gtattttatt tttgaaaatt acttttatta
     3361 tcaaagtata aggaatgttt ttgaggctgt acagaaacgc gcgggcggtg tatgctccgt
     3421 ttaaatagat tcttaaagta tgaatgttca aaaccaaacc cgaaacccta taaaaggaac
     3481 actgcaatga ctgatttttc cgtttgggaa gctgctccct ttggcgcaac cgttgatcat
     3541 atcctgcaac gctaccacaa tgtccaccgc gcacaatttg aagaattggt gccgctggcg
     3601 cagaaagtgg cccaagtcca tgccgatacc tttccggcgg aaattgccgg gctgcttgcc
     3661 gatatgcggg acgagctgct gatgcatatg atgaaggaag agcggatgct gtttccgatg
     3721 attaatcagg gtgtcggacg cggtgcggcg atgcccatca gcgtgatgat gcacgaacat
     3781 gaagaacacg accgcgccat cgcacggctc aaagagctga ccggtaattt ccacgcaccc
     3841 gaaggggctt gcggaagttg gacgcgcctt tacgcgctgg caaaagaaat ggcggacgat
     3901 ttgaacgacc acattcattt ggaaaacgat attctgtttg cccgcgtact cgattcttaa
     3961 gggataggag tcggatattt caatgccgtc tgaaatacgg tttttccgtt tcagacggca
     4021 ttttcatgtt tcagcccagc aattcgggcg gaatgcggca cacaggaatc gggttgattt
     4081 tgtgctgcat cgcgcggtga agccgcgtat agatttccag aacttcgcgc cgccgtcctt
     4141 caaaatcttc gggtttgcgc gtgccgtaca cgcccattgc ccactccagt tcgggatagc
     4201 ttgcgcccat ctgttcctcg tcggtgcgtt ccgtatccca caggccgtcg gtcggcgggg
     4261 ctttttgaat cgcctcgtcc acgcccaatg cttcggcaag ccggtaaacc tgcgttttgg
     4321 tcaggtcggc aatcgggctg atgtccacgc cgccgtcgcc gtatttggta aaaaaaccca
     4381 cgccgaaatc ttcaacctta ttacctgtcc ccgtaaccag cagtccgtgt atctgcccgt
     4441 agtagtacag ggtcagcata cgcaggcggc ttctggcgtt ggcgagcgaa agcggctgat
     4501 tggcaaatgc cgtctgatga acgccgacgg tttgctcaaa ggtttggaag gtgtcggtca
     4561 gattggccgt ttgcgcgctt acattggcat attgcccttg cagattgcgg atgtgccgcc
     4621 ttgcccgctc gagctggccg gggtgttggc gtatcggcat atccagaagc agcgtggtgc
     4681 ggccggtgcg ggcggcgagc gtggagacga cggcggaatc gatgccgccg gaaacgccta
     4741 cgacgaaccc ttttgcattt gcctgagcgg cgtattcgtc caaccatcgg acaatatgtg
     4801 tgataactgc ctgcgtatcc atttttcctc ctgtcgaaaa tatccgcatc atagcctgcc
     4861 ggcggtgcaa tttgaataga ttgagggcgg gttgatgaat atttttccca ttccccgctt
     4921 aaaaaagtca tataggaaaa ggctggcggc aagatggcgc gtgttataat attgttgaca
     4981 atttagacgg cacgatgccg ccttgggctt caaaacatat tcatattttt tattatttga
     5041 ttgattcgat gtctgatttt tttcattctg acgtgttgtc cgtttccgag ttgaacgcat
     5101 ttgccaaaag tattttggaa aaccaccttg ccggcctgtg gattgcgggc gaagtgtcca
     5161 atctgacccg cgccgccagc gggcattatt atttctcact caaagacagc cgcgcgcagg
     5221 tgcgttgcgc gatgttcaag ggtgcggcgg cgcgtttggc gcaacctttg aaagaaggcg
     5281 accatatcga agtggcgggc aaaatcagta tttacgaggc gcggggcgaa tttcagatta
     5341 ccgtaaacga agtacggctc aaaggtttgg ggcagcttta cgaagcctac gagcggttga
     5401 aggcgcagtt gcaggcggaa ggcgcgtttg cggcggaacg caaaaaacct ttgcccgtcc
     5461 gtccgcaatg tatcggcatc gttaccagtc tggcggcggc ggctttgcgc gatgtcgtaa
     5521 ccaccttgaa acgccgcgcg cccgaaatcc ccgtcatcgt ttatccggcc gccgttcagg
     5581 gcgcgggcag cgggtttcag attgcccaag cgattaaaac cgcatcgcaa cgcgccgaat
     5641 gcgatgtgct gattgtctgt cgcggcggcg gcagcatcga agacttgcgg gcgtttaacg
     5701 aagaaccggt cgtgcgcgcc atcgaagcct gcacgattcc cgtcgtcagc ggcgtgggac
     5761 acgaaaccga tttcacgctc gccgatttcg ttgccgacgt gcgcgcgccc acgccgaccg
     5821 gcgcggcgga actggtcagc cccaaccgcc aagaatcact gcaccgcctc gtccaagccc
     5881 aaggccgtct gaaaaccgtt ttggagcaac gctatttcga tgccagccaa aaactcgact
     5941 ggctcgcgcg gcaaatccgc cacccgcgcc aaaaacttga cgagcagcgc gcttcaatcg
     6001 gcaagctggc gcaaacgctg tcttactcga tgacgcaaaa cctccgcgcc cacaccgccc
     6061 gttttgaacg ccaaacccaa gccctgcaac attgccgtcc tgatgtttcc gtttaccggc
     6121 aagatatcgt ccgccttcag acggcattgc ccgccgcctt cagccggctg ctcgcccgtc
     6181 gccgtcaaag cctgaccgcc caagccgcat tgctcgaagc cgtttcgccg cagcacattc
     6241 tggaacgcgg cttctccgtt gtcaaaaata cacgcggaca agtcatccgt aacgccgatg
     6301 ttttgaagca ggggcagaag ctgcacatta ccttctccga cggcgaaacc gacgtgcgcg
     6361 taagcaaaga acaagggcag caggatttgt tcgattgcat ttaaacccaa tcggaaaaac
     6421 cgctccccct ccgccgtcat tcccgcgaaa gcgggaaccc agtcccttga ccttaagata
     6481 atctttaatt cgtcattcct gcaaaagcgg gaatccggaa gcggaaagcc gcaggaattt
     6541 gtcggaaaaa acagaaacct ctccgcacac ccgaaatgtt tcagacggcg catcttgggg
     6601 cggataaaaa cccaacccga tattttttta taatttttaa taagtttcca ttgggcaaat
     6661 gaaattttat ttttcaactg acagaaagag gatgttatga agtttttaga ccgtgaggca
     6721 agcattgcca agccgggttt caaccgttgg ctcgtaccgc ccgcagcatt agcggtgcac
     6781 cttgccatcg ggcagattta cgcctattcc gtattcaacg cgccgctgac caaacttatc
     6841 ggcataaccg aatcggcggc cggagattgg aagctgacga ccgtaggttg gattttcagt
     6901 atcgcactcg cgatgctggg cgcgtcggcc gccctgttcg gcacatggat ggaacgggta
     6961 gggccgcgca aagccatatt tgccgccgcc tgctgtttca gcctgggctt cttcgtatcc
     7021 gcattcggcg tgcgtacgca caacctcttc ctgctttatt tgggcaacgg cgtcatcggc
     7081 ggcgtaggct tgggtttggg ctacgtcggg ccggtttcca cgctgatgaa atggtttccc
     7141 gacaagccgg gtatggcgac cggtttggcg attatgggtt tcggcggcgg cgcaatgctg
     7201 gcctcgccgc tttccgtatc actgatgaac gccttttcaa acgccgcttc ggtcggggtt
     7261 gccgaaacct ttgccgtatt gggcctgttt tacctcgcac tgatgatgtt cggcgcgttt
     7321 accatccgcg tacctgccga cggctggaaa cctgaaggct ataccgtgcc gaaaacgcaa
     7381 aacaagccgg tcagcagcaa tcatgtcaac gtgtcccaag ccatgaaaac gccgcagttt
     7441 tggctgttgt tctgggtatt gtgcctgaac gtaaccgccg gcatcggcgt attgggccag
     7501 gcatccgtga tgattcagga actcttttcc gaaacctccg caggcaggca ggcggcagtc
     7561 ggcgcaggtg cggcggcagg cttcgtcagc ctcctgagcc tgtttaatat gggcggacgc
     7621 tttttgtggt cgagcgtttc cgacaaaatc ggacgcaaaa acacctacac catcttcttc
     7681 gtactcggtt cgctgctgta tttcgccgtt ccctccatcg gcgaaggcgg cagcaaagcc
     7741 ctgttcatca tcggcttctg cgtgattatt tccatgtacg gcggcggatt tgccgccatt
     7801 cccgcctacc tgaaagacct gttcggcacc tatcaggtcg gcgcgattca cgggcgcatc
     7861 ctgctggcct ggtcaaccgc cgccgtcatc ggcccggtac tggtcaatta catacgccaa
     7921 agccaaatcg acagcggcat accggcggca caggcataca gcgtaaccat gtacattatg
     7981 gcgggattgt tgattgtcgg actgttgtgc aacctcgccg tcaaatccgt acacgaaaaa
     8041 caccacgaaa aagacatcaa aaccgccgca cgcagcggca accccgacga cgaaaccgcc
     8101 atatccgacg cttaccttgt gggtgaaaaa gtttccggcg acggcatatc ggtctggtgg
     8161 cgttgggcat tggccgtcat accgctcgcc tacggcgtgg tgatggtctt tgtcaaagca
     8221 ctcgaccttt tctcctgaaa acgcccgaat aaaatgccgt ctgaaacagc aggtgtttca
     8281 gacggcattt ttactttcag gcttttttgc ggcggcgttc gcccggcagc agcatatccg
     8341 cccatttgag gatgttggcg acttcggcgg ggttgagttc gtagaactgt ccgcgtttga
     8401 ggcggttggg cagtccgatg gggccgaagg cgacgcgcac gaggcggctg actgtgaggc
     8461 cttggctttc gaatatgcgg cgcacttcgc ggttgcggcc ttctttaatc acgatgttgt
     8521 accatttgtt tgcgccttcg ccgccttgtt cgtagatgcg ttcgactttt gccaagccgt
     8581 cttcgagcat cacgccttct tcggtgagac tgcgcatttg ttcggtagtc agcccgccca
     8641 gtacgcgcac ggcgtattcg cgttcgactt cgaagctggg gtgggcgaaa cgttggacga
     8701 gttcgccgga ggtagtgaga atcagaagtc cgctggtgtt gatgtccaag cgtccgatgg
     8761 cgacccagcg gctgctggcg gcctgcggca ggcggtcgaa tatgctgacg cggccttgcg
     8821 ggtcgtcgcg ggaaacgatt tcgccttctt gtttgtaata caggatgatg cgcggcaggc
     8881 ggtcggccca tttgagtttg atgatgctgc ctttgacggt aacgtggtcg tcgggggtaa
     8941 ctttgtcgcc cagttgcgcg gttttgccgt tgaccgttac ccagccgttg ttgatccatt
     9001 cttccatttc gcggcgcgag ccgacgccgg aggctgcaag cactttttgc aggcgttcgg
     9061 gttccatacg cgacaggtcg ctgcggcgtt ctttcaaatc gcgcgcgtgt tccataattt
     9121 tttggttggg attgcggacg acgagttttt tggctttggc ggcgcgctgt ttgggggcgt
     9181 tttgcagctt gatgccgtct gaagcttttt gtccgtaagc ttgggcggcg gttttgcgcg
     9241 tttcgtcttt gggacgggct ttgcttttga aaggtttggc ggttttcttg gcagacgggg
     9301 ctgcgccgtc gcgccattgg cgtttgctgg tgggttgctt ggacatcatt tttcctaact
     9361 cgcctgataa ggcggttctt ctgcggcgtg tgcggcagga cgggttgata aaatgccgtc
     9421 ctcggtcgga cggttcgata ttgtacctga tgcggcggtt ttgcggatga aatcgtcgct
     9481 gcggctttgg ggacaaagtt tgtgcaacgg ttgaaatcgg tttgcgtgcg ggagtagaat
     9541 ctttcaaatg ataagaggag cggtaaatgg atttgcacaa tattcgggaa gattacagca
     9601 aacgggaatt gtcggaagcc gattgcgccg ataatccgat cgagcagttc gagcggtggt
     9661 tggacgaggc ggtacgcgcc gaggtcaacg agccgacggc ggtcaatgtg gcggcggtcg
     9721 acgggcgcgg gaggcccaac agccgtatgg tgcttttgaa ggaggttaat tcagaaggtt
     9781 ttgttttctt tacgaattac cacagccgca aggggcgttc gctggaactc aatccgtttg
     9841 cggcgatgac gtttttttgg ccggagctgg agcgtcaggt gcgcgtggaa gggcgtgtcg
     9901 gaaggctggc ggagaagttg tcggacgaat attttgagag tcgcccgtat cagagccgtt
     9961 tgggtgcgtg ggcaagcgcg cagagcgagg tgattccgaa caaggcggta ttggtggcaa
    10021 aggcggcggc ggtcggactc aaacatcctt tgcacgtgcc gcgtccgccg cattggggcg
    10081 gctatattgt gattcccgat ttgattgagt tttggcaggg caggccgagc aggctgcacg
    10141 accgcatcca ataccgtttg ctggatggcg gctggatacg cgagcggttg tcgccttgaa
    10201 caggaaaaaa catatgccgt ctgaaaaccg gaatcaggtt tcagacggca ttttttcacg
    10261 gggaaaatac ggacgggaag ttttttgtgt tccgaacgga aagcccgaat cctctttata
    10321 ccgaggaatt atgatgcgga cggcgtgaat gcgtcggaga aaaacaagtt ttccggcagc
    10381 ttatgctgtt gcacaaacag attttttgct tgttcggtca tagccggaga gccgcaggca
    10441 aacacttcgt attccgacag gtcggggtgg tcttgtgccg cgatgtcttg tacgtggcct
    10501 tttcttccct gccagccctc tccgggacgg gacaatacgg gggtgaagca ggtgtttttc
    10561 agacggcatg ccaacccttg tgcttcttcg agggcataca aatcatcttg atgacgcgcg
    10621 ccccagtaga aatgggcagc gcggctgctg ttttggcgga taaggtcgag caggatgctg
    10681 cggatggggg cgtagcctgt gtcggttgcc ggcaggatga cgggtttgcc gctgtcttcc
    10741 tgcaaggtaa acgaaccgag cgggccttta acgcggacga tgcctttttc tttgactttg
    10801 ggttcgctgc cgaaaatcat ttccgagcag acaccgtttt cgcgcctgcg gatgtgtagt
    10861 tccaaaatgc cttcttggtc gggcgaattg gcgatggagt agctgcggct gacgttgccc
    10921 ggcagcagta aatcaatgta ttgcccggcg tagaaggcaa acggcggggc tttgggcagg
    10981 gcaagtttca ggagggcgac atcgtgtttg aaaaccatac tttcgatgcg tgcgggcagg
    11041 gtgcggacgg gtagggcatc ggctttgcag ccggggatgt tgatgttgat atcgctttgc
    11101 gcggtggtgc ggcacatcaa aatcttgcct tgcgcttttt ctgcttcgga taaagcctgt
    11161 tccgagtgtc cgcccatttg aatatcgccg ctggccagtt cggctttgca ttgtccgcag
    11221 acaccgtttt tgcaggaatg gggcagattg aggttttgac gggcggcagc ggacaaaacg
    11281 gtttcgccgt cgccggcggc aaaggtggtt tggtcgggca gggtaacggt gtggttcatg
    11341 gaatattgtt ttttatgctg ttttaatgtg gtcagactgt gaaaatgccg tctgaacgtc
    11401 ggatttgggt ttcagacggc atcttgctta tcaggcaagt ttggctttaa tcaactcctg
    11461 cacctgcgcg ggattggctt tgcctttgct ggtcttcatc acttgcccca caatcgcatt
    11521 cagggctttt tcgttgccgg atttaaactg ttccacggct ttggcgttgt ttgccagcac
    11581 ttcgtccacc atcgcttcaa ccgcgccggt atcggtcatc tgttgcaaac tgtgtttttc
    11641 gatgatttcg gcaatgctgg tttccggttc cgcccacatg gcttcaaagg cttttttcgc
    11701 cagtttgccg cttaatgtgc catcggcaat tttgcccacc agcgcggcaa ggcgtggggc
    11761 ggtaatcggg ctgtcggcaa gttccatgcc ttctttgttc agcgtagcgg caagttcgcc
    11821 gttcatccag ttggcagtcg gcttgccttg tccgctctct ttggcggctt cttcaaaata
    11881 ggcagcctgc acgcggctgg cggtgagcag gcgcgcgtcg tattcggaca cgccgtaatc
    11941 cgccacgaaa cgcgccgcca tttctttcgg cagctcgggc atttctgctt tggctttttg
    12001 catttgggca tctgaaatga tgacgggcag caaatcggga tcggggaagt agccgtagtc
    12061 gtgcgcgtct tctttcaggc gcatcacgcg ggtttcgcct ttttccgggt cgaacagcat
    12121 cgtcgcctgc tgaactgtgc cgccgtcttc caaaatctcg atttgcgctt cggcttcgta
    12181 attaatcgcc tgatccaaga aacggaagga attgaggttt ttaatttcgc ggcgcgtgcc
    12241 gaattccgct tggcctttcg ggcgaacgga tacgttggca tcgatgcgga acgaaccttc
    12301 cgccatattg ccgtcgcaaa tgtccagcca ggttaccaag ctgtgtaagg ctttggcgta
    12361 ggcaacggct tcggcggcgg agcgcatttc gggttcggat accacttcca acagcggcgt
    12421 gccggcgcgg ttcaggtcga tgccggtcgc gccgttcaag ccctcgtgca cggatttgcc
    12481 tgcgtcttct tccatatggg cgcgggttac gttgatggtt ttcacatcgc cgccgaccac
    12541 gatttccaat ttgccgtgtt cgacaatcgg caaatccaac tggctgattt gatagccttt
    12601 cggcaagtcg ggatagaagt agtttttgcg gtcgaacacg tttttccggt tgatttttgc
    12661 atccaaagcc aaacctaatt tgatggcttt ttcaacgact tcgcggttca ttaccggcag
    12721 tacgcccggc agcgcgcatt ccactacgct ggcgtgcgcg ttgggttctg cgccgaatgc
    12781 ggtcgatgca ccgctgaaga ttttggattt ggtgttcaat tggacgtgga tttccaagcc
    12841 gattacagtt tcccaagtca taggaggttc tttctctaaa atatcaattc gatagtcttc
    12901 gcatcagact gtccggcgga gcggtttttc cgtttggatg ccgtctgaaa tgccgcgtgc
    12961 ttctgttttc agactgtcct tcatctttgt aaccatatcg tccagcattt cataacgctt
    13021 gcggtacatc gaacgctttt tgctttcgat gtcggcaagg tctttgcggg cgggggtttg
    13081 gggaagatgc cagtagccgt cccgttccaa acttgcgccg tattcctgcc agaatgcgtc
    13141 gtaattcatt ttgacgcgct ttttaagttt ccagcgtaat ttgacttgat gtttttgtgc
    13201 aatgcctatc gcgccgtcta attttaatgc ggcggcgaaa tattgcagtg cggttaccat
    13261 cagttgttgg ggacgtaagc cgtgaagttg tttggttatg cggcggacgg tgtctttggc
    13321 ttcttcacct gacggccctt gtacggaggc tgtcagcagg tgtgtgccga caaaggcgaa
    13381 ggtcgccata tacagccggt tgcctgcttc atcgcgcaaa gataaagacc acgcgccttc
    13441 ttcgacgcag ttgtcgttgc ggttgagcca caagctgatg ccgtctgaaa ggtgcgccaa
    13501 gaccagatgg aagcggcagt cttccatttg gcacagaacg tctgtgccga agagtttttc
    13561 cgccatgaga aaatctgccg tcatcgcatc taaacggcgt ttgcagttaa aacgcctgtc
    13621 aacaaattcg cgcagcagcg gataggcatc ctgcgggcag tggaggaaca gcccctgacg
    13681 gataggggag gcgttgacaa actgctcgaa ggcgtaaatt tctttttttc cgaaaaattt
    13741 tcgcaatgtg tattttaaat gttgcagccg atataaaggt gcatagctgt aaacggtgcg
    13801 aaaatcgggg aaggtaaagg tgcggttctg cttcattcgg gtcgtttgcc gtgccaatcg
    13861 ctgttgagtt ggatttgatg cgccgcaccg aggattttgg cttcggcgaa gtagttgccg
    13921 acaagctgta caccgacggg cagtccgccg ccgctgaagc ctgcgggcag ggtcaatgcg
    13981 ggcagtccgg cgaggttgac ggcgatggtg taaatgtcgc tcaagtaggt ttcaaccgga
    14041 gaagtatccg ctccgatttt gggtgcggcg gagggtgcgg tcggcgcgag gatgaggtcg
    14101 caccgtgcaa atgccgtctg aaagtcatcg gcaacgaggc ggcgcagttt ttgggctttg
    14161 agataatagg catcgtagta gccgtgcgac agtacgtaag tgccgatcat gatgcggcgt
    14221 ttgacttcgc tgccaaaacc ttcggcgcgg gttttgccgt acatttcttc caaatcgccg
    14281 aattgggcgg cacggtgtcc gtaacgtacg ccgtcgtaac gtgaaaggtt ggtgccggct
    14341 tctgcggagg cgaggacgta gtaggcgggg atggacagct tggtttgcgg cagggaaact
    14401 tcgaccggtt ccgcgccttg tgctttcagc aaatcaatgg tgttttgcaa tgccgtctga
    14461 acatcggtgc tgttgccttc gctgaagtat tctttgggca ggccgatttt cacgcctttg
    14521 agcggtttgt ccaaatcgcg ggtgtagtct tctttttcgc gttcgaagct ggtggagtct
    14581 ttggggtcga aacctgccat tgcattcaac agaatcgcgc agtcttcggc ggtttgcgcc
    14641 atcgggccgg cttgatcgaa gctggaggcg taggcgacca taccgaagcg ggaaaccgtg
    14701 ccgtaggtag gtttgatgcc ggtaatgccg cagtgcgatg cgggttggcg gatagagccg
    14761 cccgtgtccg aaccgagcgc ggcaggggcg aggcgcgcgg caacgacggc ggcggaaccg
    14821 cctgacgaac cgccgggtac gtgttccgga ttccacgggt ttttggccgc gccgtagaat
    14881 gagttttcat tggtcgaacc catagcgaac tcgtccatgt tggtgcggcc gagcgttacc
    14941 ataccttcgt cgagcaggtt ttggacgacg gtggccgtgt agggggagac gaagttgtcg
    15001 agcattttgg acgcgcacgc gctgcgccag ccggtttggc agaaaatgtc tttgtaggcg
    15061 acgggaacgc cggtaagtgc cgaagcgttg ccctgtgcga tgcgttcgtc ggcggcacgg
    15121 gcttcggcga gggttttatc ttggtcgatg gtgatgtagc cgttgagggc gggatttttt
    15181 tcggcaatgg cggcaaggta tgcgcttgcc agttcgacgg cggaaatctg tttggactgc
    15241 aacaggctgc ctgcctgttt caatgtgtat tgggtcataa atcttccatc cttaaagggc
    15301 ttcgggcggt attgctgatg ccgtctgaag cgtattcgga ttattcttcg ataacttgcg
    15361 gtacgatgta caggcggttg cgtacttccg gcgcaacggc ttggtattcg gcggcgcggt
    15421 cggtttcggt tacttcgtct tcacgcaggc ggagggcgac ctcgtgcggg tgcgccatcg
    15481 gctcgatgcc gtctgtgttg atgttctgca tctgttcgac catagtgaaa atgtcgttta
    15541 attcttgaag cgatttttct ttttcttccg cagtcagctg cagtcgggag agtcgggcga
    15601 ttttgtctac gtcggcaagt gtcagtgcca tgaaaagcat ccttgtctga tggtaaagat
    15661 accgtcgttc gggtatgatg gggcgtattg cttggatttt taattataac ggaaaaatcc
    15721 gttgcggcgg caatttgcag catcggatgc ctgccgcacg ggtttgaaag aggggaaata
    15781 atgggattgg gtatggaaat cggcaagctg attgtggctt ttttggtgct gatcaatccg
    15841 tttagcgcgt tgtcgcttta ccttgacctg accaacggac acagcacgaa ggagcgcagg
    15901 aaggtcgcgc ggacggccgc cgtcgccgtg tttgccgtga ttgcggtatt tgcgctgatc
    15961 ggcggtgcgc tattgaaggt tttgggcatc agcgtcggtt cgtttcaggt cggcggcggg
    16021 attttggtgc tgctgatcgc catttcgatg atgaacggca acgacaatcc cgccaagcag
    16081 aatctcggcg cgcagccgga aacggggcaa gcgcgccccg cccgcaatgc aggggcgatt
    16141 gccgtcgtgc ccatcgccat accgatcacc atcggtccgg gcggtatttc gaccgtgatt
    16201 atttacgctt cggcagccaa aacgtacagc gatatcgcgc tgattatcgc ggccggtttg
    16261 gtggtcagtg cgatttgtta tgccatttta atcgttgccg ggaaggtcag ccgcctgctg
    16321 ggcgcgacgg ggctgacgat tttaaaccgc attatgggta tgatgctggc ggcggtatcg
    16381 gtggagatta ttgtgtcggg actgaaaacg atattcccgc aactggcagg ttgattcgat
    16441 gtttcacagt gtcaatgtat ttacgggcga aacgctttac cgccgccccg atcaagatta
    16501 tgcggagttt gaacggcggc tggcggattt gaaaatgcac ggcagggcgt tcgcgcaatt
    16561 gggcgtaacc gaacgtgccg cgcgtctgca aaaatttgcc ggccgtttgg aggcggaaaa
    16621 agagcgtttt gcggaaatgg tgtgcgaaga agtcggacgc tgcctgcacg aatgccgtgc
    16681 cgaaatcgtc aagtctatcg aactgatacg ctattacgcc cgcctcgccc ccgaactgct
    16741 cgcccacaaa accattgcga cgcaggcgag tttgagtcag gtgcgcttcg agcctttggg
    16801 cgtggtgttt gccgtcatgc cttggaacta tcccgtctgg caggtattgc gttttgccgt
    16861 tcccgctatg tgcgcgggca acgcgtgcgc cgtcaaaccc gcgcccagcg tggcacgcgt
    16921 cagtcgggcg ttgttcgatt tggcttcaga cggtattcct ctggcaggcg cgtggctgga
    16981 tgaggcggac acgctcaagg cagtcgaaga taccgatgcg atggcgttta ccggttcgac
    17041 gcatacgggg cgcattcttg cggcacatgc gggcgcaaac ctcaagaaaa ccgtgttgga
    17101 gctcggcggc agcaacgcct ttatcgtgat gcccgatgcc gatttggaac gcgccgccgc
    17161 agaagcctgt tattcccgtt tccgcgatgc ggggcaatcg tgtaacgccg ccaagcgcat
    17221 catcgtaacc gaagccgcag ccgaccgctt tattcagctg tttctcgccg aatgcgccaa
    17281 attgaaaatg ggcgacccga aacatcccga taccacgctt gcaccgctgc accgcgaaga
    17341 tttgcgggac cgggttcacg ggcaggttga agatgccgtt tcaaacggcg cggtgtgcct
    17401 gaccggaggc aaagttccac aggggtcggg gtggttttac cctgcgacgg ttttagacag
    17461 ggtaaatcct gcctgccgcg tctggagaga agaagtattc ggccccgtcg ccctgatttt
    17521 gcgcgccgaa aacgaagaac acgccatctg ccttgccaat gattccccgt tcgggctcgg
    17581 tgcctgcatt tataccgccg atactgaacg cgcctggcgg tttgccgaaa aaatacaggc
    17641 cggatcggtg tttatcaacc gccataccag cagcgatttg cgcctgcctt tcggcggagt
    17701 caaagattcc ggttacgggc gcgaactgtc cgaattcggg ttgtacgagt tcgtcaacgt
    17761 caaaacctac tggcagaaat aaacaaaccc cgtgccgctt caggcggcat cgggtttgcg
    17821 ctagagccga ttaacggcag tatttgttta cggcgttatt gtatttacgg atcaaatcat
    17881 ccttgttttt cgcatttgag tttcccaccg ccttcaggtt catttttgaa atccggcagt
    17941 tttcttcttt gttctgccgt tcggtttcgg caattttttt cttttcttcc tcaagctgcc
    18001 cgtttttctc ggcgatatcc ttttcgtttt ccttcgcact gtccgcattc gtatcgacgg
    18061 caggtttggg cttgaccgcc ggtttggttt ggagcgtccg caggttgagg atttggctct
    18121 ggtcgggatg aagctgtttc ggcacatccg aatagctgtt gccgccgccg tctttccaag
    18181 taaagacggc ggttccggca ggcgggacgg gcaggaaaga ggcgagggta aatgtgatga
    18241 cggataaagc aaagttcatt agggtttcct tgatggctga ctgcccgggc agaatcggga
    18301 ttggttcggg cggtgtcttt tccgggttcg ggtcaaggca gggaagacac tccgccttaa
    18361 tatggtgctt tatatattgg ggtaaaacag attcccaaaa atatcattgg aatatggtta
    18421 tgctaccgta tattttggca aagaaaaaga aaattgcagg caggcgcgta taaaatccga
    18481 tgaggcgcgt gattctgcct ttggtgttga atcggttgaa tctgcggttt ttgatatttt
    18541 taattgatga aatgccgtcc gaacgctttg aaaagggttc tgacggcatt ttgttcaaac
    18601 cggtttattt gcgttgcaat acggcggcga agaagccgtc ggtttggtgt cgggcggaat
    18661 cgaggcgcag gtatttgccg gtatccaaat cgaccttcag gttttgcagc agttcggcgc
    18721 agttgacggg ttcaaattcg ggatgttcgg acaggaaacg tttgatttgc agttcgtttt
    18781 cttccggcaa cacgctgcag gtggcgtaaa ccaaacagcc ttgcggtttc accagtttgg
    18841 aggcggcatc gaggatactg tgttgctgtt ccaaaagttt ggcgacggtc tcggcggatt
    18901 ggcggtattt gaggtcggga ttgcggcgca gtgtgcctaa gccggagcag ggcgcatcga
    18961 ccaacacgcg gtcggctttg cctgccagtc gtgcgatacg ggtatcgtgt tcgctgccga
    19021 tacgttcggg gtggatgttg gtcagtccgg cacgggtcat gcgcggtttg aggttggcaa
    19081 ggcgtttttc ggcgatgtcg aaggcgtaga ttctgccttt gttggccatt tgggcgccga
    19141 cagccaaggt tttaccgccg gctccggcac agaaatccac aatgatttcg ccgcgttttg
    19201 cgcccaccaa taaggcaagc agctggctgc cttcgccttg gacttccagc gtgccgtcca
    19261 aaaacagttc gtgtttgtta agcgcgattt tgtttttcag gcggatgccc caaggagaat
    19321 aaggcgttgc ctctgcatcg gggctttcgg cttgcaacaa tggcaatact ttgtcgcgtt
    19381 tgcctttcaa ggtattgacg cggatgtcga gcggcgcagc ttggttgatg ctgcggccga
    19441 aagcgaggat ttcttcttcg ctccaatgct gctgcagttg ttccaccagc cattgcggca
    19501 attctgcggc ggtattcagg ccgtctgaaa actcggtttt acgggctttc aaattgccga
    19561 aaaactctgt ttcttcttca tcaagcaggt ctttgatttg gctgatgttg gtacttctgc
    19621 cgagaaccag cgcggcgagt gcggctttgc gcggctgcgc gtgcggacgg cgcagggcgg
    19681 tactgatttt ttgatagtgt cgcaacgcgg caaaggcggt ttcggcgatt tcgtggcggt
    19741 cttggcggcc gagctttttg tgtttgcgga aataggcgga gaggacggca tcggcaggct
    19801 gtttgaaagt cagcatttca gccaaaactt tggcggtatg gtcgagttgt gcggcgttca
    19861 taatgtaatg ggttctctta aagttggtaa agggtttcag acggccttca taatacgttc
    19921 gatttcgtgc caaaagccgt ccggccgaca ttccaaaacg gcaatcaagc cttggtcgat
    19981 gcggcggatt tcggttgcgt ttaatgtttc ggtttcggcg acacgcgcag gcgcgagata
    20041 gaccatgcgg tcgagcaggt gatagcgcgc ttcttggcgt tcaaacccgt attccgccca
    20101 atcttgaata tagatgccgc gccagccgta tggattaagc ggcggctcaa aagcatggaa
    20161 accttgtgga aaaaatccga tgccgcgcac gatggaagca ggaagtggat tttccggcag
    20221 gtcttgtgcg gctaaggttg ctttgccttg cgacgtatgc accagctgga attgctgcac
    20281 caatttggcg gctttgtccg tcagcgtgtc tttgggattg agaccgcgca ggttctgcac
    20341 ttggtctccg ccgtagtatt tacacgccag ctcgatatga tagggctgtt ggttgaggga
    20401 aacgacaaaa tccgcggcgc ctaaagtttg gccgtctgaa aaaacggtca gattatatgc
    20461 gtgcagtttg gtgtgcggtg cgttggtaaa ccaaaaagcc agcagctctt cggcataaat
    20521 gccgagacgg tggtcgaacg gggcgcgttg tgcaaggtaa tccgtcagcg gcgcgggatc
    20581 ggcatccaat gctaaaaggt agcggaaacc gcgttccccc agtagttcgc gcacgctcaa
    20641 ttcgcaaccg ctttgccaca aaggcggcgc agtcagcagc gaggcgaggt cgcggacggg
    20701 ttggctggtg agtttccacc ataatgcgtc tagggcgtaa ttcatgctta tccgaatatc
    20761 agtcatgccg tctgaaaaag cttgtaatgt tttcagacgg ccttaatcag tcttttttat
    20821 ggatacggtg gctgacggta ttgaaaaaac cgcgcaggat gtcgatgtct tcggtttgcg
    20881 tgttggcgcg tccgaacagg ctctgcatac ggcgcatcag acgctcgccg ttgcggcggt
    20941 tgaaaaagcc gatgtcgtcc atcacgcttt ccatgtgggc gagcatgcct ttgatttgct
    21001 cgtgggttgc ggcgtggtct tcctgttgaa gatgggtcat gggcgaatcg gtttggctga
    21061 aaatttcgta gcacacgacc tgcacggctt gggcgaggtt gagcgagaaa tagtcggggt
    21121 tgccgttgat ggtcatcagt cggttgcacg ctcggacttc ttcgatgctc aagccgaaag
    21181 tctcgttgcc gaagaccagc gccactttct cgccgcggtt ggcggcctgc agtaattcgg
    21241 gcaccaaatc gcgcggggtt tgcagcggcg cggtgatttc gcggcggcgg ctggtcaggg
    21301 cgcaggcgat ggtggtgtcg gcaagggctt cgtccagtgt ggcgacgatt tcggcgttgt
    21361 gcaatacgtc cgccgcgccg gaagcgagga tgaaactttc ttcaggcagt gcaaaacttt
    21421 gcacgtcatc gggattaaaa acgggcggat tttccgtcat cggcgttgcc atcagattgg
    21481 gggtgacgat ggtcagcctg tgcagaccca tcgttttcat cgcgcgtgcg gcagagccga
    21541 tgttggcggg gtggctggtg cgtgtgagga tgatgcggat gttgccgagg tagtcgggca
    21601 gggcgggttt gagagcagtc atgttcggtt ttcggtcgga atggaatagg gtataatgcg
    21661 ccgtcttttt cgttcggacg gtgaaatgcc gtttgaactg ttctttaaca acgtcggaaa
    21721 tttaacgttg cgcctgaagc agacacgcgt caggcggtat tttacccgat ttggcagggt
    21781 ttgccccaac cagataaact ccgattttgc cgctccgaag gactgcacgt ccgaaccggc
    21841 ggtatgccac ccttaaggaa atctgatgaa tccgtttttg aatacagcct ttaaagccgc
    21901 ccgccgtgcc ggtcagatga tgattcgcgc cgcaggtaac ctcgatgccg tcaaaaccga
    21961 cagcaaagcc ttcaacgatt ttgtttccga tgttgaccgc aattccgaaa tcatcctggt
    22021 tgaggctttg aaagaagcct atccgcatca caaaatcact tgcgaagaaa gcggctccca
    22081 cggcaaagct acagccgagt acgaatggat tatcgatccg ctcgacggga cgaccaattt
    22141 ccttcacggc catccccaat acgccatctc tatggcactg ctgcacaaag gcgtgttgca
    22201 ggaagctttg gtgtacgccc ccgaacgtaa cgacgtgtac atggcttcgc gcggtaaagg
    22261 cgcgttgctt aacgaccgcc gcatccgtgt ctccaaccgc atcgaattga accgctgcct
    22321 gatcggtacc ggtttccctg ttgtcgatca aagcatgatg gacaaatatc tggcgatttt
    22381 gaaagacttt ttggcaaaaa ccgccggaga ccgccgcgaa ggcgcggctt ctttggattt
    22441 gtgcgccgta gcaacaggac gttttgacgg ctttttcgag ttcaacctca aaccgtggga
    22501 cattgccgcc ggtgcattga ttgtccaaga agcaggcggc atcgttaccg atatgtcggg
    22561 tgaggacgct tggttggaaa gcggcgatat tgtggctgcc aatcccaaag tactggcgca
    22621 aatgttaaaa atcatttccg cacacgttta aatttcagcc agctcccgga aatgcgggaa
    22681 aacgggcggg tatgccgtct gaagtatcgt gccgatattt cagacggcat ttttattggc
    22741 ggcggggcag gaaggcggaa acggtcaaat gcccgaaatt gccgggaatg gaaatccgta
    22801 tctggttaaa cggagaaccc tgtgcaattt gtcccttttc tttcaattgt ttaaaggcgg
    22861 tttgctggaa tctaaatcgg ctgtggcaga aaacagacac cggggaataa aa
//
LOCUS       DAXDSD010000023        22848 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXDSD010000023 DAXDSD010000000
VERSION     DAXDSD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22848
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.023"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(41..211)
                     /locus_tag="ACFB2Q_000698"
     CDS             complement(41..211)
                     /locus_tag="ACFB2Q_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFC0917773.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            complement(344..1426)
                     /locus_tag="ACFB2Q_000699"
     CDS             complement(344..1426)
                     /locus_tag="ACFB2Q_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC0917774.1"
                     /translation="MNAQTLLANVAELVKTKLKPLVDDIDRKGYYPEAFMRELGAIGA
                     VGIEAEGGNGLGLATRIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDKYLA
                     DILQGKVLAGTGMSNTVKHLAGIEKHNLQAECVDGGYKVNGALPWVSNIGEDHIWANT
                     AQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPEQFA
                     GYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLDGAW
                     AETERLAGLAWDNTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQRRVG
                     EAMFVAIVTPAIKHLCKEIAAIEAAK"
     gene            complement(1564..2112)
                     /locus_tag="ACFB2Q_000700"
     CDS             complement(1564..2112)
                     /locus_tag="ACFB2Q_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC0917775.1"
                     /translation="MARLTVHTLETAPEAAKPRVEAVLKNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIRIIAARTNQCGFCVAGHTKLATLKKLLSEQSLNAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNRQQAVEVVMGVALA
                     TLCNYANNLAQTEINPKLQAYA"
     gene            complement(2252..2623)
                     /locus_tag="ACFB2Q_000701"
     CDS             complement(2252..2623)
                     /locus_tag="ACFB2Q_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC0917776.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            complement(2808..4499)
                     /gene="dld"
                     /locus_tag="ACFB2Q_000702"
     CDS             complement(2808..4499)
                     /gene="dld"
                     /locus_tag="ACFB2Q_000702"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC0917777.1"
                     /translation="MNASQLISSLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNGCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            complement(4596..5015)
                     /locus_tag="ACFB2Q_000703"
     CDS             complement(4596..5015)
                     /locus_tag="ACFB2Q_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917778.1"
                     /translation="MKKLLMITLTGMLAACSTGVNVGRLMVEMPQGERSVVVQVPATN
                     NPLSDAVAVGMIKTSGSPSASNMIEMLGADNINVGVAGGSQMFNKATALYSLNHAKKV
                     GNNVSVYMTGDSESDKADLENAANAKNIKLHYFFNQK"
     gene            complement(5238..5334)
                     /gene="ffs"
                     /locus_tag="ACFB2Q_000704"
     ncRNA           complement(5238..5334)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFB2Q_000704"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5595..9428
                     /locus_tag="ACFB2Q_000705"
     CDS             5595..9428
                     /locus_tag="ACFB2Q_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC0917779.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWHILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQADVLIAAAEKAVELFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRSVEYVELAGLYGRHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTAATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            9505..10506
                     /locus_tag="ACFB2Q_000706"
     CDS             9505..10506
                     /locus_tag="ACFB2Q_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC0917780.1"
                     /translation="MIDGQTTEPKQKTRIIPAPMRGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTGKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIHDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSTWIGQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEMDV"
     gene            complement(10567..10749)
                     /locus_tag="ACFB2Q_000707"
     CDS             complement(10567..10749)
                     /locus_tag="ACFB2Q_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917781.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(10922..11053)
                     /locus_tag="ACFB2Q_000708"
     CDS             complement(10922..11053)
                     /locus_tag="ACFB2Q_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917782.1"
                     /translation="MSETAKIKLNDRPENAEKPNEKVELPIVDNDKKGGHGEGGCCG"
     gene            complement(11312..12456)
                     /locus_tag="ACFB2Q_000709"
                     /pseudo
     CDS             complement(11312..12456)
                     /locus_tag="ACFB2Q_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(12781..13257)
                     /gene="greA"
                     /locus_tag="ACFB2Q_000710"
     CDS             complement(12781..13257)
                     /gene="greA"
                     /locus_tag="ACFB2Q_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC0917783.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            complement(13521..13649)
                     /locus_tag="ACFB2Q_000711"
     CDS             complement(13521..13649)
                     /locus_tag="ACFB2Q_000711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917784.1"
                     /translation="MAKRKYKDKNSLHYTGANAVWKISGLAFSVLKYVNLVFYAEN"
     gene            13648..14949
                     /gene="aroA"
                     /locus_tag="ACFB2Q_000712"
     CDS             13648..14949
                     /gene="aroA"
                     /locus_tag="ACFB2Q_000712"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC0917785.1"
                     /translation="MTESVRLPAASLKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGGYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHIGKRQDCGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(15027..15560)
                     /locus_tag="ACFB2Q_000713"
     CDS             complement(15027..15560)
                     /locus_tag="ACFB2Q_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC0917786.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(15617..17143)
                     /locus_tag="ACFB2Q_000714"
     CDS             complement(15617..17143)
                     /locus_tag="ACFB2Q_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC0917787.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(17255..18796)
                     /locus_tag="ACFB2Q_000715"
     CDS             complement(17255..18796)
                     /locus_tag="ACFB2Q_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFC0917788.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSSYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELALSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            18944..19723
                     /locus_tag="ACFB2Q_000716"
     CDS             18944..19723
                     /locus_tag="ACFB2Q_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC0917789.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREINV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            19720..20421
                     /locus_tag="ACFB2Q_000717"
     CDS             19720..20421
                     /locus_tag="ACFB2Q_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC0917790.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            20418..21872
                     /locus_tag="ACFB2Q_000718"
     CDS             20418..21872
                     /locus_tag="ACFB2Q_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC0917791.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(22021..22440)
                     /gene="fitB"
                     /locus_tag="ACFB2Q_000719"
     CDS             complement(22021..22440)
                     /gene="fitB"
                     /locus_tag="ACFB2Q_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HFC0917792.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(22440..22676)
                     /gene="fitA"
                     /locus_tag="ACFB2Q_000720"
     CDS             complement(22440..22676)
                     /gene="fitA"
                     /locus_tag="ACFB2Q_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HFC0917793.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
BASE COUNT         5276 a         6111 c         6214 g         5247 t
ORIGIN      
        1 cggaacacgc cgagggattt tccaaggggt gcggctgccg ctatatcacg aaatccaaca
       61 ggtagaaatc ttctttgccc acgccgcatt cggggcattt ccagtcgtcg gggatgtctt
      121 caaactttgt cccgggggcg atgccgtgtt ccgggtcgcc gagttcttca tcgtaaatcc
      181 agccgcaggg gccgcacata tattgagcca tttgtgtttc cttgtttgta ccgtgctttt
      241 ttgcagcggg ccgccggggt ttggttcgga tgccgtctga agcgtgccgc cgcatctgtc
      301 ccgccttggg cggtttgtgc ttcagacggc attttccgcc tgtttatttt gcggcttcga
      361 ttgctgctat ttctttgcag aggtgtttga ttgcgggggt tacgatggcg acaaacatcg
      421 cttcgccgac gcgcctttgg gcgggactcc gcatcaggta gcctttcgcg ccggaatgca
      481 gggcggcgga ttgcgcagcg gcaagggcca gtacggcggc ggcttcgcgc agcttgaggg
      541 tggcgaggtt gtcgggcgtg ttgtcccacg ccaatccggc gagccgttcg gtttctgccc
      601 acgcgccgtc cagccttgct ttgaggctgt cgtagccgtc gtcgagatag ctgttgactt
      661 ctgcattgac gatgttggcg aggcggatga tgccgaggct gccgtctatc acgcccgcgc
      721 cgatgccgat ttgcaggagg ataaagcctg ctttgatgct ttggatgtag ccggcaaact
      781 gttcgggcgc ggcgatgatg tcttcgtcgg ggataaatac gtctttgaag ttcaggctga
      841 aggtgcgcgt gccttcgagg gcgcaaaatt cggggcagtt ttgcaggctt acgccttccc
      901 tttgcccgcc tgtgatgaac atgacgtagc cgccgccgat ttgggcggta ttcgcccaaa
      961 tgtggtcttc gccgatgttg gacacccacg gcagcgcgcc gttgactttg taaccaccgt
     1021 ccacgcactc ggcttgaagg ttgtgttttt caatgccggc aaggtgtttg acggtattgg
     1081 acattccggt gcccgccaat actttgcctt gcaggatgtc ggcgaggtat ttgtctttga
     1141 cggcttggtt gggcgtttgg tgcagatacc acgcgcaagc cgcctgacac cacgcgctga
     1201 aggaggtcgc gccgcattct ttgccgattt cgcgcaagac ggcgattcgt gttgccaagc
     1261 ctaagccgtt gccgccttcg gcttctatac cgactgcgcc gattgcgccg agttcgcgca
     1321 taaatgcttc ggggtagtat cctttgcggt cgatgtcgtc gacgaggggt ttgagcttgg
     1381 ttttgacgag ttcggcaacg ttggcaagca gggtttgggc gttcatcttt gttccttaag
     1441 gtttttgggg ggaaatcggg ggcacgcctg atgcgcggct gcttttcccg ccgcttgggc
     1501 ggcttgtttt tgtttgaggg ggtgtttttg aaaaaccgcc tgcattcagg cggttttgtt
     1561 tgcttaggcg tatgcctgca atttggggtt gatttcggtt tgggcgaggt tgttggcgta
     1621 gttgcacaaa gttgccaagg ctacgcccat tacgacttcg actgcctgct gccggttgta
     1681 gcccgcatcg aaaaatgctt tgagttcgtc gtcggatact gcgccttttt tcgccattac
     1741 ggcttgggtg aaggcggcaa gcgcgccgag tttggcatcg tcaaattcac ctgccgccaa
     1801 agcgcgggcg gcattgaggg attgctcgga caggagtttt ttcagggttg cgagtttggt
     1861 gtgccctgcc acgcagaagc cgcattggtt ggtgcgtgcg gcaatgatcc ggatgacttc
     1921 gacttcgccg gcggtcaggc tgttggcggc gttgagcttg ccgacttctt ggtaaaacgc
     1981 caaggcttcg ggggcgtttg ccaatacgcc gatgaggttg gggataaagc cgttgttttt
     2041 gagtacggcc tctacgcgcg gtttggcggc ttcgggggcg gtttcgaggg tgtgtacggt
     2101 taaacgtgcc attttctttc cttgttcgca aatattgggg acgggcgcat ggtacgcatt
     2161 tcggaacgga ataggaaaga ctgtgccaca aacaaaaagt tataagaaat gccgcctgaa
     2221 gcctttcaga cggcattgcc gggaaacacg cctacccgcc cccgcgtccg aaatattgcg
     2281 ccaatgcggc ttgggggttg gcggcttcga ctgtttcggg caattcggta tagccgcgcg
     2341 ataaaaggtg gcgtgcataa aggctgttgc cctttgcgcc gaaccagacg gaaaccgcca
     2401 agcccaatac gttaaacgcc gagtctgtgg cgggcgtgtc gccgtaaacc agttgggcaa
     2461 acaccgccaa cacgaatatc gcgcccgtca tccccagccc caaaccccac attcttttga
     2521 aacacgccca cagcgtgccg aacaaaagac ccggccatga ccagccttgt ttgacggctt
     2581 ggggcggcag ggcgggatgg gtgtagattt tgtatggttt catcgtgttt ccttttcggt
     2641 tgaaaccccg ccctttgggg cggtaggatc agactttatt tgtgagacgc gcgcccgtgt
     2701 gcagacatac ggcttgaaca tgcccgtcgg ggaagacaga tccgacaatg ccgtctgaac
     2761 ggtgttttgc cttcagacgg cattgcctgc cttcaaagcg gacgcgctta ttccgcccag
     2821 tttttctttt tgctggtttt gccgataccc gggttgaagc tgttggtcgg gtcgagtttg
     2881 cggtaaaact gtttaagcgc gggcttggct tcatacaaat ggccgacgtt gtgttcggcg
     2941 ggatattgcg cgccgcgttg gtctaagaga tgcagcattt cgtgttccaa cgccatacag
     3001 ccgttgcctt ttttgatgat gtaatcctga tggaaaacgt ggcacataaa atgcccgtaa
     3061 tacaatttat ggatgatttt attgtcgatt tccggcggca gtttttcaaa ccagtcgcgg
     3121 tcgtcgcggc gcagggcgat atccagcgcg accaagtcct ccacttcgtc gtcgtgcacg
     3181 gaacggtagc ggatggcggc ggaagcgacg gcgaaacggt gcagcatggc ggcttgggtt
     3241 tcttcggcgt tgcactcgaa aaacgcgccg ccgtggtgtg caaaatactc tttcagaaac
     3301 gcgcgcgcct catccacgcc ttttccgccc attttcagaa tcaggtggtg ttcgtatttg
     3361 tcgcggtaat cgcgcatgga tttgggcagg tggtcgggca ggaatttgct gacgaactgc
     3421 attgccttgt cggaaaaatg tttgggcagg aagctgactt ttttgccgaa cctgtccacg
     3481 cgcgccttca agtcaaataa tttcggcagt tggtgcgtac cgaatttttt gatgacgtag
     3541 aacgtgtctt tgccgtacac gtcggcaatg tcgaaagcgt ggcggtggat gtattcgccg
     3601 gaaacgggca ggctttcaaa ttcgcccaag gcggcgcggc ggatgtcggt cagctcgttg
     3661 atgtcgttcg tgccgatgta gaacacggcg gtttgttttt cttgcgggaa ggtgtccaaa
     3721 cggacggcaa aaaccatcag cttgcccgcg cagcccgagg cttcgtaatg gcgggcgggg
     3781 tcggcattga aacgcgcggc ggtcggttcg tccacttggc ggacgtgttc gcaataggcg
     3841 tggtcatgtc ctttgcccgc gtcttgtttg atgtcttttt tctgataatg atgaccttgc
     3901 aggttggtca ggatttcttc gggcgtgtcg cccaagtcta tgcccaagtg gttgaccagt
     3961 tccaacctgc cctcttcgtt gatttgggcg aacaacgcca tttcggtgta ggccgggccg
     4021 cgctgtacca acgcgccgcc ggagttgttg cacacgccgc ctaaaacgga cgcgccgatg
     4081 caagaagagc cgatgaccga atgcggttct cgtcccaaag gtttcagcag cagttcaagc
     4141 tggttcaggg tcgagccggg caggcagacg acttgttcgt tgttgttgat ggtttggatg
     4201 atgttcatcc gcatggtgtt cacaatcacg atgtcgcggt cgtaatcgtt gccgtcgggg
     4261 gtcgaaccgc cggtcagtcc ggtattcgcc gcctgcgtaa tcacaatcac gtccgcttcg
     4321 acgcaggcct gcagaatttt ccacatttcc aaaatgcttc ccgggcgcac caccgccaac
     4381 gccttgcctt cgccgaaacg gtagccttgg cggtattgtt cggttttcgc ggggtcggtg
     4441 atgatgtatt tttcgcctac ggtttgggtc aggctgctga ttaattgcga ggcgttcata
     4501 acactctcct atcgggataa gcggcggcgc aaatcccggt acgccgccgt atgttcaatt
     4561 tggatatttt ggtgcggcaa gttgcacgct gcaaattatt tttggttaaa gaaataatgt
     4621 aatttaatat ttttggcatt tgccgcgttt tccaaatcgg ccttgtcgct ttcgctatcg
     4681 cccgtcatat agacactgac attatttccg actttctttg catggtttaa ggaataaagt
     4741 gcggtcgcct tattaaacat ttggctgcct cccgccacgc cgacgttgat attgtccgcg
     4801 ccgagcattt caatcatatt tgatgccgaa ggcgatccgg atgttttaat cattccgaca
     4861 gccaccgcat cggaaagcgg gttattcgtc gcgggaacct gcacgacaac agagcgttct
     4921 ccctgcggca tttcaaccat caaccggccg acattgacac ctgttgaaca agctgcaagc
     4981 ataccggtga gggttatcat tagaagtttt ttcatttgcc tactccttta gtctttaatc
     5041 aaaccgccct gttcgtcata taacttgaaa ttgaaccata cttccatttt tatgaagccg
     5101 gttcttcatt ggcctgtatg gatacattgt aatgatgcgt tatggcaaag taaaggaaat
     5161 gccgtctgaa cggctttcag acggcatcgg tgcgatacgg gaacgccgga acatcgaagc
     5221 tccggcgttt caaataaggc ggcgggccaa acccccggca ctggcgcatc ggagtgggct
     5281 gctggcttcc gcccctgacc cggtgttccg atttgccatg cggggagacc cgcctcagag
     5341 aaacggcatt ataacgggtt ttctgaaaaa ctcaaccgtt ttgatacggt catacgccgg
     5401 aaacaccgcc taaaatttat atttgataat attgtcaaca atttctcaaa gcgttatttt
     5461 gtttctataa gggtatttcc tgtttcggca ttgaaaaata tcaaaaattg aactacatta
     5521 tcgccttttc aaacccggct gaaaccgact tttcaaacgg cattcaaata aaaactgcca
     5581 aacacggaca caccatgacc acgactaccg ccccgcagcg tattcgggaa atcccctaca
     5641 actatacttc ctacaccgac cgcgaaatcg tcatccgcct gctgggcgac gaggcgtggc
     5701 acattctgca ggacttgcgc ggacagcgca aaaccgggcg ttcggcgcgg atgctgtttg
     5761 aagtgttggg cgatatttgg gtggtcgtac gcaatccgta tctggtcgat gatttgctgg
     5821 agcatccgaa acgccgcgcc gcgctggtgc gggaaatgcg ccacaggttg aacgaaatcc
     5881 gcaaacgccg cgacgataat cagcaagcgg atgtgttgat tgcggcggcg gaaaaggcag
     5941 tggagctttt tgacggcagc tttgacgaaa cgcgccaaaa gcggcggcag attttggagc
     6001 gtttgagcaa aatcaccaag ccgcacaata ttatgtttga cgggctggcg cgggtaacac
     6061 acgttaccga tgcgaccgac tggcgcgtcg aatatccgtt tgtcgtcgtc aatcccgaca
     6121 cggaggcgga agtcgcgcct ttggtgcgcg ccttaatcga gctggatttg gtcattatcc
     6181 cgcgcggcgg cggtacgggt tataccggcg gcgcggtacc tttggacgca aacagcgcag
     6241 tcatcaatac cgaaaaactc gacaagcatc gcagcgtcga atacgttgag ctggcaggct
     6301 tgtacggcag acatccgatt atccggtgcg gcgcgggcgt ggttacgcgg cgggtggaag
     6361 aaaccgcgca tcaggcgggc ttggtgttcg ccgtcgatcc gacttccgcc gacgcgtcct
     6421 gcgtgggtgg taatgtggcg atgaatgcgg gcggtaaaaa agccgtattg tggggtactg
     6481 ccttggacaa cctcgcctac tggaacatgg ttaaccctca aggcgaatgg ctgcgtatcg
     6541 aacgcgtgcg ccacaatttc ggcaaaatcc acgacgaaga aactgccgtg ttcgacgtgc
     6601 acacgctgga ttcagacggc atcaacatcg ttaaaaccga acgcttggaa atccccggac
     6661 acaaattccg caaagtcggt ttgggcaaag acgttaccga caaatttttg agcggactgc
     6721 ccggcgtgca gaaagaaggt acggacggca tcattaccgg cgttgccttc gtattgcaca
     6781 aaatgccgaa atacacgcgc accgtgtgta tggagttttt cggcacggcc gccaccgcca
     6841 cgccttctat cgtcgaaatc cgcgattttc tgctcgcaca cgacagcgtg cggctggcgg
     6901 gtttggagca tttggactgg cgttatgtcc gcgccgtcgg ctacgccacc aaagcggcgg
     6961 gcaaaggacg accgaaaatg gtcttgctgg cggacgtggt ttcagacgac gaagccgccg
     7021 tagaggcagc cgccgaacac atctgcgaac ttgcccgcgc ccgtgacggc gaaggcttta
     7081 tcgccgtatc gcccgaagcg cgcaaaacct tttggctcga ccgcagccgc accgccgcca
     7141 tcgccaaaca caccaacgcc tttaaaatca acgaagacgt ggtgattccg ctggagcggc
     7201 tgggcgagta ttcggacggc atcgaacgca tcaacatcga gctttccatc caaaacaaac
     7261 tcaaactctg tgccgctttg gagcaatatc tttcgggcaa actccccatc gacaaaatgg
     7321 gcaccgacct gccgaccgcc gaactgttgg gcgaacgcgg caaacacgcc ctcgcccacg
     7381 ttgccgccgt caaagcgcgt tgggactggc tgctcgccca tttggatgcg ccgcttgccg
     7441 actacaaatc acgctacggt acagccgtcc acgccgcgcc cgaagccaaa gacgatgaaa
     7501 gctgcttcac cgccttccgc gatttccgcc tgcgcgtgtc tgtcaaagca gacgtaatga
     7561 agccgcttgc cgaaatcttc agcggcaaaa ccgataccaa aattatccaa ggcttgggca
     7621 aaatccacgc caaaaccgta cgcagccgcg tctttgtcgc cctgcatatg catgccggcg
     7681 acggtaacgt ccacaccaat attccggtca actcagacga tgccgaaatg cttcagacgg
     7741 cctaccgttc tgtggaacgc attatgaaaa tcgcccgttc gctcggcggc gtgatttccg
     7801 gcgaacacgg catcggcatc accaagctcg aattcctcac cgatgaagat ttgcaaccgt
     7861 tttggaacta caaaaaccaa gtcgacccca agcacacctt caaccgccac aaactgatga
     7921 aaggttcgga cttacgcaac gcctacacgc cgtctttcga gctgttgggc gcggaatcgc
     7981 tgattatgga aaaatcagac ctcggcacga ttgccgattc cgtcaaagac tgcctgcgct
     8041 gcggcaaatg taaacccgtc tgctccaccc acgttccgcg cgccaacctg ctgtacagcc
     8101 cgcgcaacaa aatcctcggc gtgggtttgc tgaccgaggc cttcttatac gaagaacaaa
     8161 cccggcgcgg cgtttccgtc aaacattttg aagaactgat ggacatcggc gaccactgca
     8221 ccgtgtgcca ccgctgcgtc aaaccctgcc ccgtcaacat cgacttcggc gacgttaccg
     8281 tagccgtccg caactacctt gccgattccg gccacaaacg ctttgcgcct gcggcttcaa
     8341 tgggtatggc gtttttgaac gccaccggtc cgaaaaccat caaagccctg cgcgccgcga
     8401 tgatacagac cggcttcccc gcgcagaact ttgcctacaa aatcggcaag cttcttccga
     8461 tcggcacgaa aaagcaaaaa gccgaaccca aggcaaccgt cggcaaagcc ccgattaaag
     8521 aacaggttat ccatttcatc aaccgcccac tgcccaaaag cgtacccgcc aaaacaccgc
     8581 gctccttatt gggcatcgaa gacggcaaaa gcatccccat catccacaac cccgccgcgc
     8641 ccgaagatgc cgaagccgtg ttctacttcc ccggctgcgg ttcggagcgt ttgttcagcc
     8701 aaatcggact cgccgtccaa gccatgctct ggcacgtcgg cgtacaaacc gtcctgccgc
     8761 ccggctatat gtgttgcggc tatccgcaag acgctggcgg caacaaggca aaagccgaag
     8821 aaatgagcac caacaaccgc gtcgccttcc accgtatggc gaacaccctc aactacctcg
     8881 acatcaaaac cgtcgtcgtc agttgcggca cttgttacga ccagcttgaa aaataccgct
     8941 tcgaggaaat cttccccggc tgccgcatca tcgacatcca cgaatacctg ctcgaaaaag
     9001 gcgtgaaact cgacggcgtg aaaggtcagc aatacctcta ccacgacccc tgccacaccc
     9061 cgattaaaac catgaacgcc acccaaatgg ccagcagcct gatggggcag aaagtcgttt
     9121 taagcgaccg ctgctgcggc gaatccggta tgttcgccgt caaacgcccc gacatcgcca
     9181 cacaggtcaa gttccgcaaa caagaggaaa tcgagaaaaa cctcaaagag ctgccgcagg
     9241 gcgaacccgt caaaatgctg acctcctgcc ccgcctgcct gcaaggactg agccgctatt
     9301 ccgacgacaa caatatgcct gccgactaca tcgtcattga aatggcgaaa cacatcctcg
     9361 gcgaaaactg gctggatgag tttgtaaaaa aagccaacaa cggcggcgta gagaaagtgt
     9421 tgctataaca acggacacgg aaatgccgtc tgaacgccga aagccttcag acggcattgt
     9481 ttgaatcaaa tacctacccg aatcatgatt gacggacaaa caaccgaacc caaacaaaaa
     9541 accagaatca taccagcccc catgcggggg ctggtagacg atgtgatgcg cgacctgctg
     9601 acacgtatcg gcggctacga cgaatgcgtc agcgaatttg tacgcattac ccataccgtg
     9661 cattcacgat ccatatggtt aaaatatgtc cccgaaatcg ccaacggaaa caaaacgttt
     9721 tccggcacgc cctgcaccgt ccaacttctg ggcagcgatg cggacaatat ggcggcaaac
     9781 gcattggaag ccgtccgatt cggcgcggac aaaatcgatt tgaacttcgg ctgccccgcg
     9841 cccaccgtca acaaacacaa aggcggcgca attcttctga aagagccgga actgatattc
     9901 cacatcgtca aaacgctgcg cggacgtttg cctgcacata ttccgctgac ggggaaaatg
     9961 cggctaggct atgaagacaa aagccctgct ttggaatgcg cctgtgcgat tgccgaaggg
    10021 ggcgcgtgcg gactgaccgt gcacgcgcgc accaaagccg agggctacga acccccggcg
    10081 cattgggaat ggataaggaa aatccacgat accgtcaata ttcccgttac cgccaacggc
    10141 gacgttttca gcctgcagga ctatatcggc atcaaaacaa tcagcggctg caacagcgtg
    10201 atgctcggtc gcggtgcggt catccgcccc gatttggcgc gccaaatcaa gcaatacgag
    10261 aacggcggac cggtcaaaga cacggatttt gccgaagttt ccacatggat agggcagttt
    10321 ttcgagctgt gcctgacaaa agaggcaaac aacaaatacc cgatcgcccg tcttaagcaa
    10381 tggctgggca tgatgaaaaa aacgttcgac ccggcgcaaa cgctgttcga ccgcgtccga
    10441 acggttaaag atgcggatga agtgcgccga atcctgaacg catttgagca tgagatggat
    10501 gtttgatgtc tcccgcgctt caaagcaaat gccgtctgaa aacctttcag acggcatttg
    10561 ttatctttat tgccgttttt cccctgcttt cggtgtattt ttgtttttca gtttcgcccc
    10621 caaacccaaa cgtttttcat aatccgattg cggggtatcg tcttcctgca taccgaacgc
    10681 gccggcattg acccacagcg acagcagcgc gacgacaaag gcgcaaaagc cgattacata
    10741 ccaaaacatt gcccctccaa atttgttaaa atcatatcaa atacagtgcc gaatttatca
    10801 caagcgcacg agcaaataaa tcttctgacc gcagtatttc attcatttac cttgaaaaca
    10861 aaaaagggca cctgaaaatt ttgcgaaact tttcagatgc cctgctttgc cgaaaaacgt
    10921 atcagccgca gcaaccgcct tcgccgtgtc cgcctttttt gtcgttatcg acaatgggca
    10981 gttcgacttt ttcgtttggt ttttctgcat tttcgggccg gtcgttgagt ttgattttcg
    11041 cggtttcgct catttcctta cctcatttgg aaaacggttt gaaagattca taaatatagc
    11101 atgtttaatc cgaatctgaa aagggaagcc gtatgcgaaa tcccgtctga agcccaatca
    11161 gggcttcaga cgggatttgc tatctttatt gccgcttttc ttccgtatcc ggatttttgt
    11221 ttggggctga agcagattgg cagtcagatt gcaatcgaag aatgaaggca agccatcaaa
    11281 aacaaagcta tccgcttcac cgccccgata tttagaattt gtggcgcaga ccaacggagg
    11341 cggcattaat ttgagtgtag ttgccgatgc cggtatttcg tttcagccaa gcggcagaca
    11401 tgatggcgga agtgcgcttg gaaaaatcgt aatcgacacc ggcgatgatt tgatcatagc
    11461 tggtatgttc gcgtttctga ctgcgttcga caaagtcgaa accatgggca tagctgatgc
    11521 gcgggactgt attaccgaag cggtaggaag cagtggcggc aatttcggtc gtactgtttt
    11581 tggttttgtc ggcattttca gacaaatcca actgagccgc caaggcgaga ttcaagccgc
    11641 cttccccata gccgcccgtc aggcggtgta cctgatggtt tttcaatgga tcggtatcac
    11701 tcgctctgcc gagcaagaac aaaaagaaag catcatgccc ctcattggcg tgtttcgcat
    11761 atttaagggc ataatttccg aaaaagccgc catttttgta attcagaccg gcataataca
    11821 catccgaccc cggcttgccg acaacagccg gaactggttt catctgatta ctttccagcg
    11881 tgaaagtagc aggcgtatag gcggacttgc tgttttgact cggaacaaat tgaatgctgc
    11941 cgctgaaacc ggaaaatccg ggggaatcgt aacgcacgga aaccggcata ccgtcgtggc
    12001 gtttgaaaat acccaattgc gaagccacat tattattgct gtcccaagga tcaatggctt
    12061 tgctggcatc gtcaaacgga ttcgcaacgc gaccggcgag cgccgtgccg aattcgcctg
    12121 ccaagccgat aaaggattcc ctgttacccc aacgggtcgc gccgccgccg gcaacggata
    12181 cgtcttgctc gagctgccaa acagccttca gcccgccgcc caaatcccca cccccttaaa
    12241 gccgataaac gagccgaaat cactgacttt cgtcctgatg cggcttttgg ccttagtaac
    12301 tgtattgccc gtttgacctt ctgagggtgg ctcggtcaac tgcagccgga tgttcctgcc
    12361 ttccacacca gctttgactt cgccgtacag gctgacatcg gcaactgccg caaacggcag
    12421 tgcggacaat acgagggcgg taagtttttt tcgcatatcg gcttcctttt gtaaatttgc
    12481 taaaaacatc gggcaaacac ccaatcagtc tccaattacc ccccgaaaaa aaaaccattt
    12541 ttcggaacaa atttcgggta aattttggaa tttttatttt gaaaaataat atttcaatat
    12601 aaaacaataa attaactttt ttgaaaatgt tgctttcccg caacatgatt taaatacaga
    12661 ccttgtcaaa gccgatgcgg cgcagacccg gatgccgtct cgttttttaa aacaccataa
    12721 aaaaacacac gcggtacttt aatttcctgc gtgtgtcgag tgtatcgaaa tcaagccgaa
    12781 tcaaatatac cggacttcga taatatcgta ttcgcgtacg ccgcccggtg cctgaacttc
    12841 cgccgtatcc ccttcttcct tgccgatcag ggcgcgggca atcggagagc cgacatagat
    12901 tttgccctgt ttgatgtcgg cttcgtcttc gccgacaatt tgataggtaa cgtgttcttc
    12961 cgtttccaaa tcttccagcg taaccgtcgt accgaacacg attttgcctt cggcgtggat
    13021 ttcggccgga ttgatgatgt gggcaacgga aagtttgtgt tccaactcgg aaatgcggcc
    13081 ctcgataaaa ccttggcgtt ctttggcggc ttcgtattcg gcgttttcgg acaaatcgcc
    13141 gtgcgatctg gcttcggcaa tcgcttcgat cacttcggga cgcgccacgc ttttgagctg
    13201 ctgcaattcc tgtttcagca attccgcacc gcgtacggtc agggggattt tttgcatcgg
    13261 tctttttctc catattccgg cacaccggtt tgcggcggca agcataccgc gtaccgtctt
    13321 gttttgtgcg tccggatatt aaaataaaaa tacaagccgc ccggaaaatc ggcggcttgt
    13381 ctgtcgttga acagcggcta ttctaccaaa ttctatgaaa ttggcaatcg tgccgtgccg
    13441 ccggcagacg cgctatgtcc gcaacaaaag ccgaaaatat gccggcaaaa aaattttaga
    13501 aacaaaaaat ttaaaaaaaa tcaattttcg gcataaaaaa ccaaattcac gtactttaaa
    13561 accgaaaatg ccaaacctga gattttccag acggcatttg caccagtata atgcaggctg
    13621 tttttatctt tatatttacg ttttgccatg accgaatccg tccgcctccc cgccgcctcg
    13681 ctcaaacctt ccaccgtcgc cctgcccggc tccaaaagca tcagcaaccg caccctgctg
    13741 ctcgccgccc tgtccgacaa tgtttgcgaa atccattccc tgctcaaatc cgacgatacc
    13801 gaccgtatgc tcgaagcact cgataaactc ggcgttcaaa tcgaacatct tgccgaaggc
    13861 cgtctgaaag tacacggcac gggcggacgc ttccccaacc gctctgccga tttgtttttg
    13921 ggcaacgcgg gcacggcgtt ccgtccgctg accgccgcgc tggccgtttt gggcggcggt
    13981 tatcatctgc acggcgtgcc tcgtatgcac gaacgcccca tcggcgattt ggtcgatgcg
    14041 ttgcggattg ccggcgccga tgtcgaatat ctcggcaacg aacactatcc gccgcttcat
    14101 atcggcaaac gtcaagactg cggcgagcgc gtgattccta ttaaaggcaa tgtgtccagc
    14161 cagtttctga ccgccctctt gatggcgctg ccgctgaccg ggcaggcgtt tgaaatccgt
    14221 atggtcggcg agttgatttc caagccctat atcgacatta ctttgaaact gatggcgcaa
    14281 ttcggcgtac aggttgccaa tgaaggctac cgcgttttca aaatccctgc cgatgcgcac
    14341 tatcacgcac ccgaacactt gcacgtcgaa ggcgatgcct ccggcgcgtc ctacttcctc
    14401 gcagccggtt tgattgccgc cacgcccgtc cgcgttaccg gcatcggcgc aaacagcata
    14461 cagggcgatg tcgcctttgc ccgcgaactg gaaaaaatcg gtgcggacgt ggtttggggc
    14521 gaaaacttcg tcgaagtctc gcgcccgaaa ggacgcgccg tccaagcctt tgatttggat
    14581 gcgaaccata tccccgatgc cgcgatgacc ctcgccatcg tcgcgcttgc cacaaggcaa
    14641 acctgcacgc ttcgcaacat cggttcgtgg cgcgttaagg aaaccgaccg catcgccgca
    14701 atggcgaacg agttgcgcaa actcggtgca aaagtcgtcg aagaagccga agcgattcac
    14761 attaccccgc ccgaaacgcc gacacccgat gccgtcatcg acacttacga cgaccaccgc
    14821 atggcgatgt gtttctcgct gatttcgctg ttgggagtac ccgtcgtcat caacgacccg
    14881 aaatgcaccc ataaaacctt cccgacttat tttgaagtgt tctcatcact gaccgaaaca
    14941 gcggaataag gcggaaaaat gccgtctgaa acccgaaaac agggcttcag acggcatttt
    15001 gccgcgattc cggcgcggcg ggcggctcat tctgtaaaaa acgtatgcgc tccaaggtag
    15061 tttttggcgt aaaacggtgt ggagagtttt tcggttttga tggttttgcc gctgccgggg
    15121 gcatggatga attcgccgtt gccgatgtag agtccgacgt gtgagtagcg gtgtgcgccg
    15181 ccggtgttga agaatacgag gtcgccggcc ttgaggcggc tgtcggggat tttgcggctt
    15241 gccgccgcca tgtcgcgggc ggtgcgcggc agcttgacgt tgagggcgtt tttgtaaacg
    15301 aattgaatca tgccgctgca gtcgaagccg gttgcggtgc tgctgccgcc ccatttgtag
    15361 ggcgtgccga tgagtccgag gctgtggagc atgagttcct gcgagccttg tgtgcggccg
    15421 atgtggctga tgcggacggc ttggatttgc cggactgtct gtttgggttt cggttggcgg
    15481 tgtttgccgg aggtcgtgcc gcatgaggcg aggagcagtg cgccgagaca gaggaaaagg
    15541 gttttgtcgg gggaaaacat ggttttttcc tttgcgggtt cggatatgcc gtctgaaggt
    15601 gtttcagacg gcatttctat aataaacctt cgatgggcag cagggatagg atttttgcgg
    15661 cgatgcgttt ccaaagtttg gcttcgggtt cgttcgggta ggtttttcgg gtggcgggat
    15721 cgtgccattg caggcggttg tgtttgtcga gggtaacgcg gtaggcgtat tcgggtgtgg
    15781 tatcggcgag ggtgcgctcc atctgttctg cgattttggg gctttcgatg acgacgccca
    15841 tttcggtatt gagccgtgcg gaacgggggt cgaggttgaa tgagccgatg aagatgcgtt
    15901 tgccgtccac aatgaaggtt ttggcatgca ggctggttac ggagctgccg gtcaggcctt
    15961 tgtcttttgt ggcggggacg gcatggttgg gttgcagctc gtagagtttg atgccggctt
    16021 tgagcagcgg ttttcggtat ttgacgtagc cggaatggac ggcggcaacg tcggtcgcct
    16081 gtagcgagtt ggtcaggacg gtaacgtcta tgccgtcctg caccagtttt gccagtgcgt
    16141 ctgtgccgga ttttgtaggg acgaaatagg gtgaaaccag atagacgctt ttttcgggct
    16201 gtttgagcgc gtcttgcagc ctcccggcaa tcggcggttt gcggcggtcg cggtcgagtc
    16261 cttttgcagg gttgtcgctg atcaggcggg tttggacgct ctgccagtcg atgcgtcccg
    16321 tctgtatttt ttggtagagg ggcgactgtt cgacggtttc gcggtagcgc aggagcgcgt
    16381 gtctggatgt ttcgtcgttg tatccgagtg cttgaagacc cttgccgatg ttgccgctgc
    16441 ggatgatgcg cgtggcgttg tgggcggaat ggcttgccca gtagcggtcg aagtcgtgcg
    16501 atacttcgcc gacgacgctg ccggtggcga ggatgtccag gtcggcgaaa acggtgtcct
    16561 caccgacttt gaagtattcg tcgccgatat tgcgtccgcc gagtatggtg gcgcggttgt
    16621 cggcggtaaa ggatttgttg tgcatgcggc ggttgaggcg ggggaagtcg gtcaggtagc
    16681 cgagtgcgcg ccatttgcgt aggacgaagg ggttgaacag gcgcacttcg atattgggat
    16741 ggctgtcgag ggcgagcagg agatcgtcca acccgcgcgt gttgttgtcg tccaacagca
    16801 ggcgtacgcg cacgccgcgt tctgcggcaa ggtacatgag gttgaacagc agcctgccgg
    16861 aaatgtcgtt gcgccaaatg tagtattgca aatcgaggct gtgttcggca gattcgataa
    16921 gggcggcgcg ggcggcaaag gcttcgtggg ggtcgtcgag caggtagatg tcggatagcc
    16981 cgttgttatg aggggtgtgc cggatttgca ggatgttgtc caggaggaca ggtttggaag
    17041 tattgaaatg acggctttcc gtccgttctt ccagtggggg caaccatgaa gaacatgaac
    17101 agagaaggag gcataaaagg gaaatgaggc tgcgtgtttt catcggggat atagtttcag
    17161 acggcattgc ctgtattttg gggtctgtat gcattgaaat gaagcatcat aatctaaagg
    17221 ttggaatggg taaaagtttt gtcgggatgc ggggttaagg ccgcgtgcct cttgtcaggt
    17281 ttgcaccgtc tgaaacgtgc agacgcttaa aaaccaacga cgaggcaagg gtgatgccgc
    17341 cgatactgag cagggtcata cggaaggcga aatgcaggcc tgaagaagcc ggtatcagaa
    17401 atgtccagtt tttaaggatt aatgcgccgg caacaatgcc cacgctgatg gcaagctgtt
    17461 ggttgaccgc catcaggctg ttgccgctgc cggtctgttg cgggcgcaaa tcggcgaggg
    17521 tcagtgtgtt catggcagaa aactgtaggg agttgcatgc gccgatcgcc agcgagagga
    17581 aaatccaaat ccacagcggc gagtttccgt caggcagggc gagcagcatg atgaaggcgg
    17641 caagcagctt ggtgttccaa agcagtaccg tgcggtagcc gaaacgtttc atgagcggtg
    17701 caatcagcgg tttgaccagc agcgaagaca gggcgacggg tgcgaccagc cagcccgaca
    17761 ggcttgcgcc gaagccgaaa gcgacttgaa acatcagggg catcagaaaa ggaatcgagc
    17821 tgatgccgag acggctgaac agattgcccg ccagtcccaa acggaaggtg cgtatcagaa
    17881 acaggtcggc ggaataaatc ggtttggacg cggttttcat atgtcggaaa taacggcgtg
    17941 caaacagcag tccgccgcac agcggtaaca gtgcaaaata cggaggcagc gcgtgcgaca
    18001 ggctttctgc cgcaagcagc agcaggcagg cggcaacgga aaaggtcaga tagcctttga
    18061 aatccagagc ggtatcgttg cctttgacat cgggcatgat gttgcgtccc aatatgaaac
    18121 ccagcagacc gatgggcagg ttgagcagga aaatccagtg ccacgaagcg tattcgacca
    18181 gataaccgcc cgccagaggt cccaacgcag ggccgatcag tgcaggcatg accgcataat
    18241 tgatggcgtt gagcagcttc gatttttcat atacacgcaa gatggtcaga cgcggtatcg
    18301 gaaccagcat cgaaccgccg atcccctgaa cgacacggga aagcgccaat tcaaacagcg
    18361 aacccgatgc ggcgcacaat gccgatccga gcataaaaac ggcaatcgaa ccgaaaaaga
    18421 cttttttcgt tccgaacttg tccgccaaat aactgctcaa aggaatcagc agggcaaccg
    18481 tcagcgtata ggcaacgact gcctgttgca tatccggagg cgattcgttc aggtcggcgg
    18541 caatttcggg cagtgcggta tttaaaatgg tcgcatccaa catctgcata aaaatggcaa
    18601 ttgccagcag aagcggcagc caaggggatg gtgcgcgggc ggacagggtg tttttttcca
    18661 tagggtgatt gtaccccatc cttgtgccgt tattgttttc agatgctgtc tgaatgccgg
    18721 cagagtcggc atcctgaatg ttcacaagca aacgaatcgg cattgcattg taatgataat
    18781 tattatcgaa aatcatcaga gtaaggtaca gtaagcgtta tggggcagtt tgtaagaaaa
    18841 accggattat tttttaaaat tagacttgac ccgcaacggt caattactta aagtaaacgc
    18901 ttacctttct acagaaaaaa cgggtttccc gttatcaaaa aatatgagcg caaccattcc
    18961 cccaaaaatc atccgatacg acagcaatcc gacagatgtc tattttttcg gcacttgcgt
    19021 ccttgatctt tttatgcccg aagcaggcat ggatgccatt gccctaatcg agcagcaggg
    19081 catacgcgtc catttcccga tggcgcaaag ctgctgcggt cagcctgcct attcatccgg
    19141 ccatccgacc gaagccttcg atgtcgccaa agcgcaactc gaccttttcc ccgaaaactg
    19201 gccgattgtc gtaccgtccg gctcatgcgg cggcatgatg aaacatcact ggcctaccct
    19261 gtttaaaaat acagaatacg aatccaaagc ggttgattgt gccaaccgta tcattgagtt
    19321 tactcatttc ttgcttgcca tcggctacaa gcctgaagac aaaggcgagc cagtcaaagt
    19381 cgccgttcac acttcctgtg ccgcccgccg agaaataaat gtccatcttt caggctggca
    19441 gttgattgac ggtatggaaa acgtcgaacg catcgtccac gaccacgaaa gcgagtgttg
    19501 cggcttcggc ggcacattct ccgtcaaaca agccgatatt tccggcgcga tggtaaccga
    19561 caaagttgcc gcgctgaaag aaaccggcgc aaccgaaatc atcagcgcgg actgcggctg
    19621 tatgatgaac atcggaggca aaatcgccaa ggacgagccg gatatgccgc gtccgaagca
    19681 tatcgcatcc ttcttgttgg aacgcaccgg aggcaaagta tgagcgcgcg cgaaaatatt
    19741 ttggcaaaac tgaaaaaagc cggcgcattg ccgatggaag agcctgcggt ttttgattat
    19801 taccgcgaaa agggcgtttc ttgggacagc gaagccgagc gtctgaaaca ttgggctgcc
    19861 gccatgcgcg cggtcaaaac cgaaatctat tgggtgacga aaagcaattg gatgcaggtt
    19921 ttccgcgaag cggcagaagg caagggttta aaaaacatcc tgctgccctt ggcgaccgaa
    19981 cacggacaaa ttgcccgtgc cgcattggcg ggcagcaata tcgacccgat tgccttcgag
    20041 cgcgaaatcg atacttggaa aaccgagttt ttcacgaaca tcgatgcagg cttcagcggc
    20101 gcgcaatgcg gcatcgcccg caccggcacg ctgatgctgt tttccagccc tgaagaaccg
    20161 cgtactttga gcctcgttcc gcccgtgcat ttctgcctgt tcgatacgtc caagatgtac
    20221 aacgagtttc acaacgccgt cgaaggcgaa aaactggtgg aaaacggtat gccgaccaat
    20281 gtattcctga tttccggccc gtccaaaacc gcagacatcc agttgacgct cgcttacggt
    20341 gcgcacggtc cgcgtgattt ggtcatcctc gccattttgc ccgaccacat ttcccctgcc
    20401 gatttggagg aaaacgcatg actacgcaaa ccatcaagtt ccacatgaag ccggaaactt
    20461 tcaaacaaaa cgccgcaatt tcccttcaag acaagccctt gcgtaaaagc ctgcgtaccg
    20521 cgatggatat gctgatgacc aagcgcaaag ccgttttgac cgacgaagaa gagttgcaaa
    20581 gcctgcgcga tttgtgcgaa cacatccgtc agcgttccct ctccaaatta cccgcgctgc
    20641 tggagcagtt ggaagaaaac ctgactaagt tgggcgtgaa agtacactgg gcggaaaccc
    20701 ctgccgaagc ctgccaaatt atccacgaca tcatcacagc caaaaacggc aagctgatgg
    20761 tcaaaggcaa atccatggtc agcgaagaaa tcgagctgaa ccattatctt gaagcaaaag
    20821 gcattaaggc ggtggaaagc gacttgggcg agtttatcgt ccaaatggca ggcgaaaaac
    20881 cgacccatat cgtgatgccc gcaatccata aaaccaaaga acaggtcagc gaactgttcc
    20941 accaaaacct cggcacaccg cttacagacg acgtggacca actgaccgga ttcgcccgca
    21001 aagccctgcg cgatatttac agcactgccg atgtcggctt gagcggcgta aacttcgccg
    21061 ttgccgaaac gggcacgctg tgtctggtcg aaaacgaagg caacggccgc ttgagtacta
    21121 ccgttccgcc cgtacatatc gccattacgg gtattgaaaa agttgtcgcc aagctgtctg
    21181 atgttccgcc tttgtacagt ctgctgccgc gttccgccat cggtcagaac attaccactt
    21241 atttcaatat gattaccggc ccgcgccgca gtgaagaatt agacggtccg caggaaatgc
    21301 acttggtttt gctcgacaac ggccgcagcc aagcttatgc cgaagaccaa atgcgccgca
    21361 ccctgcaatg tatccgttgc ggcgcgtgta tgaaccattg cccggtttat acacgcatcg
    21421 gcggcgcggc atacggcaca acctatcccg gtccgattgg cgagattatt tccccgcacc
    21481 tgttgggtct tgatgccacc cgcgacctgc cgaccgcctg cacgatgtgc ggcgcgtgcg
    21541 tggaagtttg tccggtacgc atcccgatta ccgaacaaat gcaacgtttg cgcgttgaag
    21601 cacaacgttc gccgaccgaa accgtgccgc accccatccg agggcaaggc gcatcgcata
    21661 ccttcggcga acaaatggcg tggcgcacat tcaacggtat tttcagcggt agcaaaacct
    21721 accgcgcctt cggttgggca gccaccaagt tccgcaatct gaccccgcgc aaacagttgg
    21781 gttggacgca aaaccgcgta ccgatgaaac cggcgaagaa aaccctgcac gaactgatgg
    21841 cggaaaaaat gcgccaaaaa gaacaggcat aaaaagttgt tcgcaaaaat accgtctgaa
    21901 acccgaaaac agggcttcag acggcctgaa aagcagcctg aacgcgcaaa aataaataat
    21961 gaaacggaat gctaagacgc tttcaggcag cctgacatta aggctgctta aaagcgtctt
    22021 ttaatcgtgc cacggattga acaccgcgac atcggccgca aaaaatgagc cggtatcacg
    22081 cgtagcaact gtcaaactgt gctgttttgc agtggcggca atatagccgt ctgcggcagc
    22141 aatctctttg ccatgtgttt tggcatagga acgaatttgc gcatagattg cggcaaccgg
    22201 ttcatcaaaa ggcagaatcc gccccgcaaa taaaggcaaa atggattgtt ccaaacgttc
    22261 gtgcagcaca ttctttttct tgccattgag caacaacgcc acacccaaac gcaattctgc
    22321 aacagtaatg gcagacaaat acacatcttc caatatcaaa ctatccaacc atgccaccac
    22381 acgttcattg ggttgtgggc gcaaaggttc ggaaatcaca ttcgtgtcca gcaaaatcat
    22441 cacaaagaaa cctcgttatc agtattacga ccgcgtacgt cttccagctc aacacctccg
    22501 atttcctgcc ctattgatgc caacattgac cccaaacgta cagtttgttg tgctttggcg
    22561 atgttatcca aaattaagcg gatttctgct tcggtactgc gccctgcggc tcgcgcacgg
    22621 aatttgattg cgttgtgcgt ggcctcggat aaatttctaa tcacaacaga agccattgcc
    22681 tttccttcct gaaaaaagtt tttcaaatgc tatcaaaata aaaaaaatga tagcaatcta
    22741 ttttttaacg cctgcggatg cctgaaaatt caggtggccg caggcgtttt tttacatcta
    22801 aaaaaacgtc tcccctccct ctttctgata aataaaaaac agtcgctc
//
LOCUS       DAXDSD010000024        22713 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXDSD010000024 DAXDSD010000000
VERSION     DAXDSD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22713
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.024"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(200..1039)
                     /locus_tag="ACFB2Q_000721"
     CDS             complement(200..1039)
                     /locus_tag="ACFB2Q_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HFC0917794.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAALAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1324..1399
                     /locus_tag="ACFB2Q_000722"
     tRNA            1324..1399
                     /locus_tag="ACFB2Q_000722"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Arg,seq:ccg)
     gene            complement(1464..2657)
                     /locus_tag="ACFB2Q_000723"
     CDS             complement(1464..2657)
                     /locus_tag="ACFB2Q_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC0917795.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRAMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="ACFB2Q_000724"
     CDS             complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="ACFB2Q_000724"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC0917796.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3912..5012
                     /gene="aroC"
                     /locus_tag="ACFB2Q_000725"
     CDS             3912..5012
                     /gene="aroC"
                     /locus_tag="ACFB2Q_000725"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC0917797.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSNK"
     gene            5103..5528
                     /locus_tag="ACFB2Q_000726"
     CDS             5103..5528
                     /locus_tag="ACFB2Q_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC0917798.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5602..7587)
                     /gene="parE"
                     /locus_tag="ACFB2Q_000727"
     CDS             complement(5602..7587)
                     /gene="parE"
                     /locus_tag="ACFB2Q_000727"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC0917799.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7654..8202)
                     /locus_tag="ACFB2Q_000728"
     CDS             complement(7654..8202)
                     /locus_tag="ACFB2Q_000728"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC0917800.1"
                     /translation="MNLTEGDTVLDREGYRPNVGIILINERNEVFWGKRVREHSWQFP
                     QGGIKPGESPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRG
                     QKQIWYLLRLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELS
                     SRFLRGMESYEDFAARQPSGNR"
     gene            8344..9639
                     /gene="serS"
                     /locus_tag="ACFB2Q_000729"
     CDS             8344..9639
                     /gene="serS"
                     /locus_tag="ACFB2Q_000729"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC0917801.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9712..10710)
                     /locus_tag="ACFB2Q_000730"
     CDS             complement(9712..10710)
                     /locus_tag="ACFB2Q_000730"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC0917802.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="ACFB2Q_000731"
     CDS             complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="ACFB2Q_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC0917803.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGGEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12001..12771)
                     /locus_tag="ACFB2Q_000732"
     CDS             complement(12001..12771)
                     /locus_tag="ACFB2Q_000732"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC0917804.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12774..13766)
                     /locus_tag="ACFB2Q_000733"
     CDS             complement(12774..13766)
                     /locus_tag="ACFB2Q_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC0917805.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WHVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTSAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13833..14516)
                     /locus_tag="ACFB2Q_000734"
     CDS             complement(13833..14516)
                     /locus_tag="ACFB2Q_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC0917806.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="ACFB2Q_000735"
     CDS             complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="ACFB2Q_000735"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC0917807.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16169..17023)
                     /gene="folP"
                     /locus_tag="ACFB2Q_000736"
     CDS             complement(16169..17023)
                     /gene="folP"
                     /locus_tag="ACFB2Q_000736"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC0917808.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADFVPPEEEEWARVEPVLAEAAGWGVPVSLDTR
                     RTVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKY
                     QDVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETG
                     LPLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKV
                     WEALGVNR"
     gene            complement(17163..18056)
                     /locus_tag="ACFB2Q_000737"
     CDS             complement(17163..18056)
                     /locus_tag="ACFB2Q_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC0917809.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18147..20249
                     /locus_tag="ACFB2Q_000738"
     CDS             18147..20249
                     /locus_tag="ACFB2Q_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC0917810.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAIGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITEPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPRLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNSEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20313..21791)
                     /gene="ubiD"
                     /locus_tag="ACFB2Q_000739"
     CDS             complement(20313..21791)
                     /gene="ubiD"
                     /locus_tag="ACFB2Q_000739"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC0917811.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21811..22326)
                     /locus_tag="ACFB2Q_000740"
     CDS             complement(21811..22326)
                     /locus_tag="ACFB2Q_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC0917812.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSGQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22333..>22713)
                     /locus_tag="ACFB2Q_000741"
     CDS             complement(22333..>22713)
                     /locus_tag="ACFB2Q_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917813.1"
                     /translation="NLIHYITLFFAAVLVFGRRDSMYWYPVLVNLMMLAVFGSSLFAG
                     QTVIEKLARLQQPDLPEKAVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTG
                     VIAYVLMGMLFAGEWLYRKLVLKV"
BASE COUNT         5044 a         6555 c         6016 g         5098 t
ORIGIN      
        1 caaatgggtt aaaccagtat ccatacatca gcttttttat catcctactt tttattcatc
       61 cgatcgggca gacagatttc aaagatgaaa agcctattct attcacaccc atttgatgtc
      121 atttccacac ggacgaacaa ataaattccc ataaaaaaac ggagcagata cctgccccgt
      181 tttttaattt aatccgaatt tagaattttg caccggattg gtttgccata tagtcaacag
      241 ccgctttgac ttcgtcatcg ctcaaacctg cattgccgcc tttggccggc atcgcgttaa
      301 agccttcaag ggcatgtttg tgcaaggttt ctttgccttt tttgatacgc ggtgcccaat
      361 cgtctttttt gcctatgccg ggaataccgg gaatcgaacc gccgtggcac acctgacagg
      421 ttgcttcgaa gactttttta ccgtcaacgc cgaccgcaag ggctgccgca cccttgtctt
      481 ctgccttcgc ttctgccgga gctgcactat cggcaggagc agaagctgtt cctgaagcgg
      541 cattgtcggc aggcgcagcc tcatcaggat tcgggaaaga accgccgctt ttgttcgcca
      601 tgtaggtaat cgcccgtttg agttcctgat cggtcaggtc tgccgcaccg cctttggcag
      661 gcatggcgtt aaagccgttc agcgcgtgtt ggaacaaggt atcgaagcct tgcgcgatac
      721 gcggcgccca gtcgccgttg tgttccagtt tcggagcgtt cggcacattg ctgtccgccg
      781 cgtggcattg gatacagatt ttgccgaaaa tctgttcgcc ttggcgttcg ccgacgggga
      841 tgccgtcacc catcgtcaat tgtccgacag gctggatgcg ggtctgcgtt gccgcttccg
      901 tagtggcatc gacatcgccg aacgatccac tgcccgccag cttaatcagg aaataaagga
      961 ctgcaataac aataacgata ccgctcacaa gggtaaacag tgcagagcct tgggctttgt
     1021 tgtcgcggag ttgtttcatt tggtaggcct cgccgtcagg ttaggttgtg ctgtagatta
     1081 tagtttggcg tgttaaacac agttaacaat attttgctgg attatactga attcacaggg
     1141 tctttccaat cgctatcatt gaaaatatga aaaaatttgc caatggtatc tgtacaaaac
     1201 aaataatccg ttgaaaatac cggattatct ccaagaaaac tccccttgtg ccgccatacg
     1261 ccgcctgccg gcgcaagata acctttgcca atttgcagaa tttacgttaa ccttacgttt
     1321 tccgcaccca tagctcagtt ggaagagtgt cagtttccga agctggaggt cacaggttcg
     1381 atccctgttg ggtgcgccaa ttataaagag gccgtctgaa agataaatat ttttcagacg
     1441 gccttttgac ttacttcaaa ttcttatttc aacacttcgg caagcgcatc ggcgacatag
     1501 gtgatatttg atgtattcag cccggcgacg cacatcctgc cggaatccag caggtaaacg
     1561 gcaaattcgt cgcgcaacct gcggacttgc cccacgctca accccgtgta gccgaacatt
     1621 ccgcgctgtt tgatgaaata agtgaaatcg cggtcgggga ttcgggcggt caatacgccg
     1681 taaagtttct gccgcatcgc acggatgcgg tcgcgcatca tataaacttc gttttgccac
     1741 aaggcgtaaa gttcggagct gttcatcacg tcggcggcga tatacgcgcc gtgcgcgggc
     1801 gggctggagt agatgcggcg gacggtgaat ttgagttgtc cgaacaccaa atccgcttct
     1861 tctttattgg ggcaaaccac gcttaagccg ccgacgcgtt cgccgtagag cgacaggttt
     1921 tttgaaaacg aattgctgac aaacagcggc aaatccattt ccaccgcttt gcggacggcg
     1981 taggcatcgc tgtccaaatc gccgccgaag ccttggtagg cgatgtccat aaacggaatc
     2041 agtttgcgcg ttttgatgat ctgcaacact tcgtcccatt gccgttccga catatccacg
     2101 ccggtcgggt tgtggcagca gggatggagg atgaggacgc tgttttcggg cagggtgttg
     2161 aaaaacgcgg tcatttcgtc gaatttcacg ccgacagtgg cagggtcgta atatgggtaa
     2221 gtgccgacct cgaaacctgc gccttcaaaa atgccgcgat ggttgtccca agtcggatcg
     2281 ctgacgtagg cgcgcgcttc gggaaaccag cggtgcagga aatccgcccc gactttgagc
     2341 gcgcccgagc cgcccagagt ctgtacggta acgatgcgtc cctgcgcgag cgcggggttg
     2401 cctttgccga acaacaaatg ctgcaccgcg ctgcggtaaa cgtccaaacc ttccatcggc
     2461 aggtagggcg acggcgctgg cgcggcggca cgggcggttt cggcacggct tacggattcc
     2521 aatacgggca ttctgccttc gtcgtcgaaa taaatgccta tgctcaaatt gactttttcg
     2581 gggcgcgggt cgtttttgaa ggtttcgacc aaactcaaaa tcgggtcgcc gggatagtat
     2641 tcgatgtgtc ggtacatagt ccttaccttt tgcttttcaa aggattttct ttttcaacag
     2701 tacgccgctt tcgatatggt gcgtaaacgg gaattggtcg aacagggcgg cacgtccgac
     2761 cgtatgggtt tccgtcaagg tgtccaaatt ggcgcgcagc gtttcgggat tgcaggaaat
     2821 gtagatgatg ttgtcgaact gcgacaccag cttcaaagtt tcctcatcga tgccggcacg
     2881 cggcggatcg acgaaaatag tggaaaatgc gtaatccgtc aaaacaatac cgctctcctt
     2941 aaggcgtgta aactcacgtt ttccggtata ggcttcggta aactcctcag cagacaggcg
     3001 ggcgattttg atgttgccta tccagtttgc ctcaatattc cattgcgccg cactgacgga
     3061 ggttttggag atttcggttg ccaaaacctg tcggaaatat cgggacagcg gcagggtgaa
     3121 attgccgttg ccgcaataca gttcgagcag gtcgccgccc aagccttccg ccgcgcggca
     3181 cgcccattcg agcattttcc ggcatacagc ggcattcggc tgggtaaagc tgccttcgat
     3241 ttggcggtag tggaaatcgc ggtcgccgac gcgcagggtt tccgtcacat agtcctgttt
     3301 taagactatt ttctgccccc tgctccgccc gataacggaa atatccaact gttgctgtaa
     3361 cgcttgcgcc gccttcatcc actcatcatc aagccttttg tggtaaatca tggtaaccag
     3421 catttccccg ctgagcgtgg acagaaattc gacggcatac caatggtttc tcagttcggc
     3481 agattgggcg gcgacggcaa tcaattcggg catcaggcag ttgactgcct ctgaagcagc
     3541 atcgaaacgg tcgcaacgga tcaggcttgc cccgctagcc ttctgccctt tttcaaacat
     3601 ggcgtaaaac atttcaccgc cttcgtgcca aatgcggaat tcggcgcgca tgcggtagtg
     3661 tttgtccgga gattcgtaca cttcccactc aggaacatcc aaacctgcaa aaagggtttt
     3721 aaggtagtct tttttacctt gaagctgctg cgtgtagtca ttcatatcgc tatcctgaaa
     3781 aatcagaccg gattataagc cgattttcct cgccgcacgc aaactctttg actgccgccc
     3841 cttcaatgac ggacgggctt ttgtgctaaa atccgccatc tttccacact ataccgataa
     3901 agggaaaaat catggcaggc aacactttcg gacaaatctt caccgtgacc accttcggcg
     3961 aaagccacgg cgcgggtttg ggctgcatca tcgacggctg cccgcccggc ttggaattaa
     4021 gcgaagcgga tatccaattt gacctcgacc ggcgcaaacc cggcaccagc cgccacgtta
     4081 cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa ggcaaaacca
     4141 ccggcacacc catcgccctc ctaatccgca acaccgacca gcgcagcaaa gactacggca
     4201 acatcgccac cgccttccgc cccggacacg ccgactatac ctattggcac aaatacggta
     4261 cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgcagcc cgcgttgccg
     4321 ccggagccgt tgccaaaaaa tggctgaaag aaaaattcgg cacggaaatc accgcctacg
     4381 ttacccaagt cggcgaaaaa aaaatccggt ttgaaggcag cgaacacatt tcccaaaatc
     4441 ctttttttgc cgccaaccaa agccaaatcg ccgaactgga acactatatg gacggtgtgc
     4501 gcaaatcctt ggattccgtc ggcgcgaaac tgcatatcga agccgccaac gtccccgtcg
     4561 gtttgggcga acccgttttc gaccgcctcg atgccgaaat cgcctacgcg atgatgggca
     4621 tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg caacgcggca
     4681 gcgaacacgg agacgaactg accccgcaag gcttcctgtc caaccattcg ggcggcatcc
     4741 tcggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa cccaccagct
     4801 ccatcgccac accgcgccga agcatcgaca ttcacggcaa ccccgtcgaa ctcgccaccc
     4861 gcggcaggca cgatccctgc gtcggcttgc gcgccgcgcc gattgccgaa gctatgctcg
     4921 ccttggtttt aatcgaccac gccctgcgcc atcgtgcgca aaatgcggat gttgccgcgg
     4981 atacgcccga tatttcccgc tcgaacaagt aaaaagattt agccaaaaca cgggctttat
     5041 actaaaatac cgaccattgc cgtgccgtct gaacgcacga ttcttagagg aatacaaccg
     5101 aaatgacaca agaaaccgct ttgggcgcgg cactgaaatc cgccgtccaa actatgagca
     5161 aaaagaaaca gaccgaaatg atcgccgacc acatctacgg caaatacgat gtattcaaac
     5221 gcttcaaacc gttggcgctc ggcatcgatc aggatttgat tgccgctttg ccgcaatacg
     5281 attccgcact gattgcacgc gtcctcgcca accactgccg ccgtccgcgc tatctgaaag
     5341 ccttggcgcg cggcggcaaa cgtttcgatt tgaacaaccg tttcaaaggc gaagttaccc
     5401 ccgaagaaca ggcgattgcg caaaaccatc ctttcgtgca gcaggctttg caacagcagt
     5461 ccgcccaagc tgccgccgaa acgccgtctg ttgaagccga agcagccgaa tcttccgcag
     5521 cagaataaat ccccaaacga aatgccgtct gaaaaccgat ttggtttcag acggcatttt
     5581 ttcgtatgcg gcaatcacgg ctcaaatatc gagttgcgcc gtatcgcctt cgcgttccat
     5641 ccaagcgcgg cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga tgtcgcgcgt
     5701 ctcgtcatcc gcgccttcgg ggatttgcac ctgcaacagg cggcgggtgt cggggtgcat
     5761 ggtggtgtct ttgagctggt cggggttcat ctcgcccaag cctttgaaac ggctgatgga
     5821 ataggcggtt tctttaacgc cttctttttg cagccgctct aaaatgccgt caagttcgtt
     5881 ttggtcgagg gcgtagaatt tgcgggcggg tttgcttttg ccttgtgcgt tgacatcgac
     5941 gcggaacagc ggcggctggg cgacgtagat gtgtccgtcg gcgaccagtt tggggaagtg
     6001 gcggtagaac agggtcaaca gcaaaacctg aatatgcgaa ccgtccacgt cggcatcgga
     6061 caagatggct attttgccgt agcgcaggcc gcttaagtcg ggatggtcgt tgatggcgtg
     6121 cggatcgaca ccgatggcga cggaaatgtc gtggatttcg gcgttgccga aaagctggtc
     6181 ggggtggact tcaaagctat tgagcacttt gccgcgcagg ggcaggatgg cttgtgtggc
     6241 tttgtcgcgg gcgagtttgg cggagccgcc ggcggaatcg ccttcgacga ggaagagttc
     6301 gttttcgcgg atgtcttcgc tttcacagtc ggtcagctta ccgggcagga tggcgacgcc
     6361 gctgcctttt ttcttttcga tttttttaac cgaacgtatc cgtgcctgtg cctgacggat
     6421 ggcgagttcg gcgatttttt tgccgaagtc cacgttttgg ttcagccaca attccaaagg
     6481 atcgcccgat acggcggcga caagtttcag cgcgtcgcgg ttggtcagct tgtctttggt
     6541 ctgaccttgg aattgcgggt cgaggacgcg ggcggagagg acgaaggcgg tttttccgaa
     6601 cacgtcgtcg ctttgcactt taacgccgcg cggtaagagg ttgtgcaggt tgatgaaatt
     6661 gttgacggcg ttgaacacgg cttgtttcaa gcctgcttcg tgcgttccgc ccagcgaggt
     6721 cgggataagg ttgacgtagc tttcgttggc gcacgagcct tcttccagcc aagtcaaagc
     6781 aaacgcggca ccttcgccaa tgctgaaatc gccgttgtgg ccgtctgaaa tgtagttttc
     6841 gcaggagaac agcggcacgg cttcctgtgc gtcggcaatc aggtcggtca gatagctttt
     6901 cagaccgtcg gggtaatgcc aggtttgggt gtgcgcttcg tcttcgcctt tgaccggacg
     6961 ggtcagggaa acgcgcacgc ccggcagcag cacggctttg gcgcgcaata gacgctcgag
     7021 ttcgggaatg ctgtaattcg ggctttcaaa atatttgccg tccggccaca cgcgcacttc
     7081 ggtgccgctg tctttgacgg cgcatttgcc cacttgtgcc aacggttcga ccacatcgcc
     7141 gccggcaaac acgatgcggt ggactttgcc gtctcgtttg acactgactt caagtcgggt
     7201 ggaaagcgcg ttggtaacgg atacgcccac accgtgcagg ccgcctgaaa aggcatacgc
     7261 actgccgccg tcttttttgt tgaacttgcc gcccgcgtgc aggcgggtaa acacgagttc
     7321 gactacgggt acgccttctt cagggtgcag gccgacggga atgccgcgcc cgttgtcgcg
     7381 cacggaaagc gaaccgtctt catgaatttg cacgtcgatt tcagtggcga aaccgcccaa
     7441 cgcctcgtcc gccgcgttgt cgatgacttc ttggcagata tgtgtcgggc tgtcggtgcg
     7501 ggtgtacatg ccgggacgtt ctttgaccgg ctccaagcct ttgaggacgg tgatgctgga
     7561 ttcgctgtat tggttgtttt tagccatagg aataatctga aagtaagaaa aacaacgctt
     7621 tcagacggcc tgaaagcgtt gcgttccgtt gttttaccgg ttgccggaag gttggcgggc
     7681 ggcaaagtct tcataacttt ccataccgcg caggaagcgg gaagagagtt ctttcaacgc
     7741 ccccaaataa acgtcgcgtt tgaaatcaat cacttcgtca acgggcgccc aatattgatg
     7801 ccaacgccag ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat cgcaatcccg
     7861 gccggtcagg cgcaggagat accaaatctg cttctgcccg cgataagagc cgcgccattc
     7921 gcggcgcacc cagttgttcg gcacgtcgta acgcagccag tcgcgcgtcc gcccgacgat
     7981 tttgacgtgt tgcggcagaa gtccgacttc ttcgtaaagt tcgcggtaca tcgcggtttc
     8041 ggggctttcg ccgggcttga tgccgccttg aggaaactgc caagaatgtt cgcgcacgcg
     8101 cttgccccaa aagacttcgt tacgttcgtt aattaggata ataccgacat tggggcgata
     8161 gccttccctg tccaacacgg tgtcgccctc cgttaaattc aatcttggga ttttcccaca
     8221 aatcggacgg ttttgacaaa tcggacggta tggttcaagc atgtcgaaac acggacggat
     8281 tcgggaaaat atcttaaatt tggtttacaa taatacgttt caaattaatt ccggaatcag
     8341 actatgttag acatccaatt gctccgcagc aacaccgccg ccgttgccga acggcttgca
     8401 cggcgcggtt atgactttga taccgcacgt tttgacgcac tggaagaacg acgcaagtcc
     8461 gttcaggtga aaaccgaaga actacaagcc tcgcgcaaca gcatttccaa acaaatcggc
     8521 gcactgaaag gtcagggcaa acatgaagag gcgcaggcgg ccatggatca agtcgcccaa
     8581 atcaaaaccg atttggaaca ggctgccgcc gatttggatg ccgttcaaaa agaattggac
     8641 gcatggttgt tgagcatacc taacctgccg cacgaaagcg tacctcccgg taaagacgaa
     8701 accgaaaacg tcgaagtccg caaagtcggt acgccgcgcg aatttgattt tgaaatcaaa
     8761 gatcatgtcg atttgggcga gcctttgggc ttggatttcg aaggcggcgc gaaactctcc
     8821 ggcgcacgat ttaccgtgat gcgcggacaa atcgcccgtc tgcaccgcgc cttggcgcaa
     8881 ttcatgctcg atacgcacac gctgcaacac ggctacaccg agcattacac gccttatatc
     8941 gttgacgata cgacgctgca aggcacaggt cagctgccca aatttgcaga agatttgttc
     9001 cacgttaccc gcggcggcga tgaaaccaaa accacgcaat atctgattcc gacagccgaa
     9061 gttaccctga ccaataccgt tgccggcagc attatcccgt ccgaacaact gccgctgaag
     9121 ctgaccgcgc attcgccctg tttccgcagt gaagcgggat catacggcaa agacacgcgc
     9181 ggcctaatcc gccagcacca gttcgacaaa gtagaaatgg tacaaatcgt tcatcccgaa
     9241 aaatcatacg gcgcgctgga agaaatggtc ggccatgccg aaaacatcct gaaggctttg
     9301 gaactgccct accgcgtgat taccctgtgt accggcgaca tgggtttcag cgcggccaaa
     9361 acgtatgact tggaagtttg ggttcccgca caaaatacct accgcgaaat ctcaagctgc
     9421 tccaactgcg aagatttcca agcacgccgc atgaaggcgc gtttcaaaga cgaaaacggc
     9481 aaaaaccgct tggtacatac cttaaacggc tccggtttgg cagtcggtcg cactttggtc
     9541 gcggttttgg aaaaccatca aaacgccgac ggcagcatca atatccccgc cgcactgcaa
     9601 ccgtatatgg gcggtgttac caagttggaa gtgaaataag ccttaaccct gaacataaat
     9661 gccgtctgaa acctgtttca gacggcattt cctttaaact cttaaaacac atcaaccgtc
     9721 ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc tcgcggatat tggacagcgt
     9781 aacttccgaa atattaccca acgcctcttc cgtcaaaaat gcctgatgtc cggtaaacag
     9841 tacgttatga caagacgaca ggcggcggaa cacgtcgtcg gtaatcacat cgttggattt
     9901 gtcttcaaaa aacagctcgc gctcgttctc gtacacatcc atgcccaatg caccgatttt
     9961 ccggcatttc aacgcctcga tggcggcggc gctgtcaatc agcccgcccc ggctggtgtt
    10021 gataatcatc acgccgtctt tcattttgtc gaacgccgct tcgttcagca tatagtggtt
    10081 ttccggcgtg gcgggacaat gcagcgtgat gatgtccgat cgggcataca gctcgtccaa
    10141 atccacatac ctgccgccga gtttttccac ttcggggttg caaaacggat cgtaagccag
    10201 caggttcata ccgaaaccct ttaaaatccg catcgttgcg ataccgattt tccccgtgcc
    10261 gataacgccc gccgttttgc cgtacatatt gaagccggtc agaccttcca gcgaaaaatt
    10321 cgcatcgcgg gtacgctggt aggctttgtg gatacggcgg ttcaaagtca gcatcagacc
    10381 gaccgtatgt tccgcaaccg attcgggcga ataggcaggc acgcgcacga ctttcaagcc
    10441 caactcttca gccgccttca aatccacatt attgaagccg gcacaacgca acgccacagt
    10501 tttcacgcca atttgcgcca atttttccaa cacgggccgg ctggcgtcgt cgtttacaaa
    10561 aatacaggcc gcttccgcgc cttccgccat tttcgccgtt ttcgcatcca gcatgaaatc
    10621 aaaaaactcc agctcgaagc cgaaatgccg gttggcgcgg gtaaaatgtt cgcggtcata
    10681 gcttttcgta ccgtaaatcg caatcttcat caatatgtcc agttgtcgtc tatggttgag
    10741 aaacggcata atacaccgaa ttcaaacaaa tcagtagaat atggcggatt aaaattgatt
    10801 tcatgcacgg catttccatt tcaaaacaca aaactcaatc gcccatcgcc gccagaagct
    10861 ccgcctgatg ttcggcaatc agggcatcgg tgatttctgc caaatcgccg tccatcacaa
    10921 aatccagctt gtgcagggtg aggttgatgc ggtggtctgt taccctgcct tggggatagt
    10981 tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat gagggatttg cgttcggcgg
    11041 cttctttggc ttggacttcg cgtttttgcg cgtcgttcag gcgggcggcg aggactttca
    11101 tcgcctgcgc tttgttggcg tgttggctgc ggccgtcttg gcattcgacc accatgcctg
    11161 tcggcaagtg ggtaatgcgg acggcggagt cggttttgtt aatgtgctga ccgcccgcgc
    11221 cggatgcgcg gaaggtatcg gtgcgcaggt cggcggggtt cagttcgatg tcttcgagct
    11281 cgtccgcttc gggcatgacg gcaacggtac aggcggaggt gtggatgcgg ccttggcttt
    11341 cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc aaatttcaga cggctgtacg
    11401 cgccgagtcc gacaatacgg gcgatgactt ctttatagcc gcccaattcg ctttcgttgg
    11461 cggacacgat ttcaacctgc cagcggttgc gttcggcgta gcggctgtac atacgcagca
    11521 aatcgccggc aaacagcgcg gcttcgccgc cgcccgtgcc ggcgcgtatt tcgataaaga
    11581 tgtttttgtc gtcgtcggca tctttgggca gcagcagttt ttgcaattcg gtatcgagtt
    11641 cgtcgatttt ggctttcgcc gcttcgattt cttcggcggc aaagtctttc atttcggggt
    11701 cggacaacat ttcttcggca tccgccaagt cgctttgggc aagccgatag ttttggaata
    11761 cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag cttgcggtag ttgtccatat
    11821 cggatgtggc ttcgggttgc cctaaaaggt gggtaacttc ttccaaccgg tcgccgagtt
    11881 gttgcagttt ttctaagata gacggcttca taattcttcc ataacaaacg ccgcctgaat
    11941 ttcagacggc atcaaaacgc ggtcattata atatattttc tgaatattca aaaagataat
    12001 ctagggacgg agcagactgt cggcattgcg ttccgttatc gcggcgattt ccgccgcgcc
    12061 ggtaccgcgt atttccgcag caatttcggc aatccgaaga atgttggcag gcgtattgac
    12121 ggtgttttcc aacatgaacg ggctatccgt ttccaatacg aaatccccgt cgttcaaggc
    12181 tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc agcagcgaac cgatgccgat
    12241 tttgaacccc agttttgtca acacgcgcgc ttcttccgcg ctgcctgaga aagcgtgaac
    12301 gatgccgcct tgagtaaagc ctgtctgttt gacggcggcg gcgatgtcgg cggtggcttt
    12361 gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg atggcaagct ggcggctaaa
    12421 aacttgaatt tgccgttcac gctgcggcgg cgtttgggtt ttgtcgtaaa aatccaagcc
    12481 gatttcgccg acccacgctc gggggtaacg caccaatacg gcttccaaac cggaaaaatc
    12541 ctgttccgca atgccgtctg aaaaccaagg atggatgccc agcgcaatac ggatttgacc
    12601 gtgttcggac ggcatttccg acagttccgc cacgtcctgc caatcctgcg gacaggtggc
    12661 gggaacgata aaccgccaca cgcccgcctg tcgcgcttcg gcaagcacct gcgccagatt
    12721 gccgcgcaaa acggaatcgg caagatggca atgtgtgtcg gtcagcctca tcttcacacc
    12781 agccccaacc gctgcaactc gtgtttcacc ccgtccgcgc cccatccttc ggaaacggca
    12841 agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc cgccgttgac cacgcccgcc
    12901 cgacgcagcg cgtcgccctg tgcgtaaacg gcggcagcgc aaccctgcgg cacttggctg
    12961 atgttaagca acaatttgcc ttgctgcgta aagtcttgca cggcgcggat gaagccttcg
    13021 ccggcggagg tattgccgtg tccgtagctt tgcaggataa gggcttgggc gggaagctgt
    13081 ccgagcccgt cggcaagttc ttggacggca aagccgggga taagcgtgcg gacggcgatt
    13141 tttgcctgcg ggtcgggata acggatttcg aggccgtctg aaacatctgc cgcgtcctga
    13201 ttcgggatgc ggacattgtg ccagccccgg gtttcgtccc actcggcaag tgcgccgaaa
    13261 tgggcattgc cgaaaccggc ggcggtttcc gtgctgattt tgctgctgcc gacggcggga
    13321 tacagtttgc cgtcaaaggc aataagcgtc tgcttcaatc cgagtgggaa tgcggcaacg
    13381 gcagtcacca gattgcgcgg cgcgtcgctg ccctctgcgt tgtacggcca ttgcgaaccg
    13441 gtcaggacga tcggtttgtc caaaccttgc agggcgaggg cgaacatatt ggcggtatag
    13501 gcgagtgtgt ccgtgccgtg cagcaccaaa acaccgtcgt actgcggcag cgcggcggca
    13561 atgatgtcca gccaagcgca ccagttttgc agttggacgg cggaagaatc aatcagcggc
    13621 tcacaaacgt gccaatcgaa atccatgccg tctgaaaacg gcgcgagtgc ctgcccgacc
    13681 agcgcggtat cggggcgcaa gcccgcactg ctttgcgtca tgccgatggt gccgccggta
    13741 taaaggacaa aaattttctg tttcatggct cattctgaaa atgcaaagac cgcctgaaac
    13801 gcgaaatgcg tccggacggt cgaaaatccg atttactgct gcttctgtgc cgcttttttc
    13861 gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca attgtgcgcg gtaaagctga
    13921 taatgtcggc gtttgcgcca ccagaaccac gccagcgccg ccgccagcac gcccaatgcg
    13981 atgaagatgc ccgattgcag gctgtgcatt ttcgccatca gccaatcgat gttgtgcgcg
    14041 ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca gtgcggccag cccgtccata
    14101 atcagaaagc gcagatacga tactttgcgg ctgatgccgg cggtaacgaa aacggcagtc
    14161 cgcaaacccg gcaggaaacg ggcgacaaac agaacccagt tgccgtattt gtcgaatttt
    14221 tcctgaacct gcgcgtaacg tttcggcgtc atgatgcgcg caatcggttt gaacttgagg
    14281 attttctgcc cccagatgcg tccggcggca aacatcacgc cgtcgcccgc caacacgccg
    14341 agcataccga ccgcaaacat aatatgcgga ttggtataac ccatacccga aatcacgccg
    14401 cccgttacca aggtcaaatc ttcgggaatc ggcacgccga aaccgcagat gaccaaaacg
    14461 aaaaacacgg ccgcatagcc gtattcgaca aaaaaggctt ccaaaagggc aaacatagcg
    14521 gatattccat tgtcggagat aaaaagtcag aacaagccga aacattttct gcgcgaaacg
    14581 gcattctatc aaagattatg ccgtctgaaa gcggcagggg gggcagattc gcgccccgcc
    14641 ggacgttcag acggcattta aacaggaaaa ccgccgctgt tgaaacggtt ttgcctgatt
    14701 ttgcctaaat gccgccgatg gcggcggcga tacgctccgc cccgtcgcgc gcccaatcgg
    14761 cctgacgcgc ctcgaccatc acgcgcacga cgggttcggt tcccgaagcg cgcaacacga
    14821 cacgcccttt gccttcgagt tctttttcca cttcggccaa cacgtctttc gaagcctcct
    14881 gccatttctg acccttttgg atgcgcacgt taatcatcgt ttgcggatac ggctgccagt
    14941 cggcgcaaac ggtggcgagg tcttggttca ggatttgcag cgccgccaag acttgcagcg
    15001 cggaaataat gccgtcgccg gtgttatgtt tgtccatgca caaaatatgg ccgctggctt
    15061 cgccgccgat gagccagccg cgttggttca gctgttccaa cacatagcgg tcgccgactt
    15121 tggcgcggca gaaatcgacg ccctgctctt tcagggcgat ttccatcgcc atattggtca
    15181 tgaccgttcc gaccacgccg ccgatgttga taccttctcg ggcgcgggct ttggcgatga
    15241 cgtaaatcag gctgtcgccg tcgtaaacct gcctgttttt atcgaccatc atcaggcggt
    15301 cgccatcgcc gtccaaggca ataccgtaat cggcttcgtt ttgcaacacg gcggcttgga
    15361 gggttttggt atgggtcgcg ccgcattttt cgttgatgtt gtagccgttg ggttcgttgc
    15421 cgatgctgac gacctgtgcg ccgagttcgt ggaacacttt gggtgcaacg ccgtatcctg
    15481 caccgttggc ggtatcgata accaacttca aaccccgaag gtcggaatgg ccggggaagg
    15541 tggatttgca aaactcgata tagcggtcgt ccgcaccgct gatgcggcgg gcgcgtccga
    15601 gacgtgcgga cggttgggtt ttcatttctt catcgatttt ggcttcgatt tccaactcga
    15661 cttcatcgga aagtttcacg ccgccttcgg caaagaattt gatgccgttg tcggaatagg
    15721 tgttgtgcga cgcggaaatc atcacgccgg cggacaggcg caatgcgcgg gtcagataag
    15781 ccacaccggg cgtgggcaac ggcccggtct gtaccacatt cacacccgcc gccgtaaagc
    15841 ccgctaccag cgcggcttcc agcatatagc ctgaaatgcg cgtgtctttg ccgatgagga
    15901 cggtcggttt ctgatcggta tcgtgctgca ccagcacctg ccccgccgca tagccgagtt
    15961 tcaatacaaa atcgggcgta atcggaaact gaccgacttc gccgcgcacg ccgtccgtgc
    16021 cgaaatattt ttttgccatc atttgctccg tgaatgtgaa ccgttgtccg agattataca
    16081 gtcagtttgc gccctgctgc ctgcaccgtt gatgccgtct gaagccgtcc cgcccttttc
    16141 agacggcatt cggcgcaaaa accgccgttt accggttcac gcccaacgct tcccacacct
    16201 tcaacgcgtc cgccgtcgcc ttcacatcgt gcacgcgcac gatttgcgct ccgcgtgcca
    16261 cggaagccaa agccgctgcc acgctgccgt gtacgcgcgc cgccgcgtcc gcctcaccgg
    16321 tcagctcacc tatcatgctt ttgcgcgaca caccgatcag cagcggcaaa ccggtttccg
    16381 ccatcaattc gggcaaatgc cgcatcagtg cgatattgtg ttgcaggttc ttgccgaagc
    16441 cgaaacccgg gtcgagcgta atccgttgcg gcgcgatgcc tgccgcgaca caggtttctg
    16501 accgtgtttt cagataacgt gccacttcgc cgaccacatc ctgatatttc ggattgtcct
    16561 gcatggtttc gggcaagccc cgcatatgca tcaggcaaat gcccgtgtcc gcctggcgcg
    16621 ccagcaattc gaccgcacct tcgtcggtca acgccgccac atcattgata atatcgatgc
    16681 cgccgagtgc caacgccttt tccataacca ccgtgcggcg cgtgtccaaa ctgacgggaa
    16741 caccccaccc cgccgcttcc gccaatacag gctcaacccg cgcccattct tcttcttcgg
    16801 gcggaacgaa atccgcaccc ggccgcgtcg attcgccgcc gatgtcgaga atgtccgcac
    16861 cctctttcaa aagccgttcg gcatgcgcca aagctgtttg ggcgttttgc gaatacgcgc
    16921 cgccgtcgga aaaagaatcg ggcgtgagat tcacgatgcc catgattttc ggtttgtcca
    16981 aaccgatttc aaaccgtcct gcccgccaaa cgtgtcgtgc catctgaact cctcccgaaa
    17041 taaaaaacag attatatgcc gtctgaaacc gtcttgtgcg cttcggacgg caccgctatt
    17101 cgggcggcag acggcatgtt gtcccaatgt ctgcgccgcc tttgaatctg ccggcatgcc
    17161 tgctatccgc ccgccttttc aaaacaggtt ccgacgattc cgcacgcgcc tgccgccttt
    17221 gccaagccgt acaggatttc ctgcggcata tcgcggctcc ataatcccgt aatattcgca
    17281 atcacgggca gatggctgat ttggcggact ttcacgatgg attcgacatc caaacggtag
    17341 ggatggcctt tggtatggcc caacacgccc gactcgccca gaatcaggtg gcggttgccg
    17401 cgcgagacga catattctgc ggcattcaac caatcttcgg cagaatggtg cttgtcttta
    17461 cacagaacca agggaatgtt caggcttccg gcttcattca atacggcaag atcggacatc
    17521 agcccgccgc ccaaatacag gatgtccgct cccgcattca aagccgcttc gacatggcgg
    17581 acgttgcgaa cgcgcaccaa tacgggtttc cctgcgccat gcgccgatgc ggcctgttcc
    17641 gccaaccgtc tgcaccgtcc ccgcccttca tccgcactta aagtgtcgta taagtttgcc
    17701 gaagtgaaaa acggatccag aaacactgca tccgcattgc tccatactga cggttctgca
    17761 gcgatacgga cggtttcccc gccgccgaaa gccacgcctt tggcggcaac gcggctgtct
    17821 tccgcccgat tttcccgact gacggttttc catgtgtcca aaatgcggac ggctttctcg
    17881 acctccggca gcgtctgtac ctccctgacg ctcaaaaccc tatcgtcgcc gattgcgccg
    17941 atgacggtac gctcgtcgcc gtgagaaatg tgttctcgca gacctctgct gcggatgaag
    18001 gcgacaacgc cggcaatgtc cgcttcggcg gcacgcctgc tcatgacaat aatcatattt
    18061 cctcctgaca caagagacgg cccgcccaaa ataggatttt tgcaagccgt gttatactgt
    18121 ggcgtgtttt acagattgtt cgggctatgg atttattatc ggttttccac aaataccgtc
    18181 tgaaatatgc ggtggcggtg ctgacgatgc tgcttttggc ggcaatcggg ctgcacgctt
    18241 ccgtatatcg caccttcacg cccgaaaaca tccgcagccg cctccaacaa agcattgccc
    18301 atacccaccg gaaaatctcg tttgatgcgg atatacggcg caggcttctg ccccgcccga
    18361 ccgtcatcct gaaaaacctg accattaccg aacccgacgg cggccgggtc gccgtttccg
    18421 tcaaagaaac caaaatcgga ttgagctgga aaaacctgtg gtcggatcgg atacaggttg
    18481 aaaaatgggt ggtttcgggt gcggatcttg ccctgacgcg cgacagaaac ggcgcttgga
    18541 acatccaaga cctgttcgac ggcgcgaaac actccgcctc agtcaaccgc attatcgtcg
    18601 aaaacagcac cgtccgcctc aatttcctgc agcaacagct tatcctgaag gaaatcagcc
    18661 tcaacctgca atcccccgat tcgtcggggc agcagtttga aagttcgggc atactggttt
    18721 ggagaaagct gtccgtcccg tggaaaagca gggggctgtt cctttcagac ggcatcggca
    18781 cgcccgaaat ctcaccgttc cattttgaag cttccacttc gctggacgga cacggcatca
    18841 ccatttccac caccggcagc ccttctgtcc gcttcaacgc cggcggagcg gatgccgccg
    18901 gcctcggcct gcgtgcagac acttccttcc gcaacctcca cctgaccgcg caaatccccg
    18961 cactggcact caaaaacaac agcatcaaaa ccggcacggt caacggcacg tttaccgccg
    19021 gcggcgaata tgcccgatgg gacggttcgt tcaaactcga caaagccaac ctgcactccg
    19081 gcatcgccaa catcggcaac gccgaaatct ccggcagctt caaaacaccg cgccttcaaa
    19141 ccaatttctc cctcggctcg ccgttggttt ggagtcggga caacgggctg gacgccccgc
    19201 gcctgcacat atcgaccctt caggataccg tcgaccgcct gccgcaaccc cgtttcatca
    19261 gccggctcga cggttcgctg tccataccga atctgcaaaa ttggaatgcc gaattaaacg
    19321 gcacattcga ccgccaaccc gttgccgcaa aattcaaata tacgcgggaa ggcgcaccgc
    19381 gcctggaagc cgccgccgcg ctgcaaaaat taaacctcgc cccctatctt gacgaatttc
    19441 ggcaacaaaa cggcaaaata ttccccgaca tcctcggcag gctgtccggc aacgtcgagg
    19501 cacacctcaa aatcggcagc atccaactcc ccggcttgca actggacgat atggaaacct
    19561 acctccacgc cgacaaagac catatcgcgc tcagccgttt caagtcaggg ctttacggcg
    19621 gccataccga aggcggcatc agcatcgcca acacccgtcc cgccacttac cgcctgcaac
    19681 agaatgcaag caacatccaa atccaaccgc tgctgcaaga cctgttcggc ttccacagct
    19741 tcagcggcaa cggcgatgcg gtcatcgacc tgaccgcaag cggcgaaaac cgcaaacagc
    19801 ttatccgctc gctgcaaggc agcctgtcgc tgaatatttc caacggcgcg tggcacggca
    19861 tcgatatgga cagcatttta aaaaacggcc tttccggaaa aatctcgggc agcacaccct
    19921 tctaccgatt cacgctcaac agcgaaattt cagacggcat cagccgccac atcgataccg
    19981 aactcttctc cgacagcctc tatgttacca gcaacggcta taccaatctg gatacgcagg
    20041 aattgtctga agatgtcctt atccgcaacg ccgtccatcc gaaaaacaaa ccgattcccc
    20101 tgaaaatcac cggtacggtg gacaagccgt ccattaccgt cgattacggc aggctgaccg
    20161 gcggcatcaa ttcgcgcaaa gagaaacaga aaatcctcga agacaccctg ctggaacaat
    20221 ggcagtggct caaacctaaa gaaccgtaaa taccctgcgt acaaaaatgc cgtctgaaac
    20281 acattaatct tcagacggca tgaccgtaaa acctacaacc ccaatttccc ccaaatccca
    20341 tcaattttaa ccgtaaccgc agggtctttt ttgattaccc gtccccattc gcggtcggtt
    20401 tctcccggcc atttgctggt cgcatccaaa cccattttgc cgccgagtcc gctgacgggg
    20461 ctggcgaagt cgaggtagtc gatgggcgtg ttttccacca aaacggtgtc gcggacgggg
    20521 tccatgcgcg tggttaccgc ccagatgact tctttccagt cgcgcacgtt tacatcgtca
    20581 tccaccacga tgatgaattt ggtgtacata aactggcgca ggaacgacca gcagcccgtc
    20641 atcacgcgct tggcgtgtcc ggcgtactgt tttttcatgc tgaccaccgc catgcggtag
    20701 gaacagcctt cgggcggcag gtagaaatcg gtgatttcgg agaactgctt ttgcaaaagc
    20761 ggtacgaaca cttcgttcaa cgccacgccc aaaacggcag gttcgtcggg cggtttgcct
    20821 gtgtaggtag agtggtaaat cgggttttcg cgcatggtga tgcgttcgac cgtaaacaca
    20881 gggaaatggc cctgctcgtt gtaatagccc gtgtggtcgc cgtatgggcc ttccaacgcg
    20941 gtttcgtttg gatgaatcac gccttccaat acaatttcag cacgggcagg cacttgcaaa
    21001 tcgctgccga tacatttcac cagctccgtc cgcgaaccgc gcagcagtcc ggcaaactgg
    21061 tattcgctca aagtatcggg aacgggcgtt accgcgccca aaatggtgga ggggtcgcaa
    21121 ccgagtacga cggcgacggg atacggcgta tcgggattga gtttgcggaa ttcctgataa
    21181 tccaacgcgc cgccgcgatg cgacagccag cgcataacca gcttgttttt gccgatgagt
    21241 tgttggcggt aaatgccgag attttggcgt tttttgtgcg gcccgcgcgt gacggtcaag
    21301 ccccacgtta ccagcggcgc aacgtcttcc ggccagcaat gttgaatcgg aagctgatac
    21361 aaatcaacgt cttctccttc ccacacgatt tcctgacacg gcgcgttttt caccacgttc
    21421 ggcgccatgc tccaaatatc tttcaacagc ggcagtttgg aaaacgcgtc tttgatgcct
    21481 ttgggcggtt cgggttcttt caaatacgcc agcgtctgcc cgatttcgcg cagcttggac
    21541 acgctgtccg cgcccatgcc catcgccaca cgttcggggg tgccgaacag gttcgccaac
    21601 acgggataat catagcgcgt accgtcgggc ttaaccgggt tttcaaacaa caacgccggc
    21661 ccttcggcgc gcaacacgcg gtcggcaatt tcggtcattt ccaaatgcgg ggaaacgggg
    21721 tgggcgacgc gcttgagctt gccctgctgc tcgagcatag cgatgaagtc gcgcaggtct
    21781 ttgtatttca tgtttatcct ttttgtcctt ttatcctgaa caatccgatt cggataccgc
    21841 ccctatcctt gcctgcgctt cggcatatcc catgccgtga taaaagtcgc gtaccggcgg
    21901 atgtccgccg ctttgatgaa gttgcagcaa agggcgttgg ttgtcggact gggtaacgac
    21961 attgcagtgc agaccgaagg tgtcggtttc ataagggggc agccgattgc aaatcatacc
    22021 gaaataaacg gcgttttcag ggttgtcgta aaagcggctt tgatagtcgt taaaactctt
    22081 ttcgctgacg gacacccaca cgccgtattc cagcgtttcc tgatgcccga taatcggaat
    22141 aggcagcgcc gcgcggataa agcggtcggt ttggccggaa tagcggatga tgcagaaatc
    22201 agaatcgcat tccgcttgat aagcaatgcg ttcttcttca ctgagttgat tatagggata
    22261 gggggcggta aagccggttg cgggcatatc ttcatgtttt tcgccgcagg aagtgcaagt
    22321 gtgcataagg tttcagactt tcaacacaag tttgcggtaa agccattcgc cggcaaacag
    22381 catccccatc aatacatagg caatcacgcc tgtataaacc gcccaccaat catatcgccc
    22441 caaccgggcc aacaaagcgg caagcgtccc gttggcaata aagaatacgc accaaacctg
    22501 cgttacccgg cgggtatagc gcacggcttt ttcaggcagg tcgggctgtt gcagccgcgc
    22561 aagtttttca atcaccgtct gcccggcaaa caggctgctg ccgaacaccg ccaacatcat
    22621 caaattaacg aggacaggat accaatacat tgaatcacgc cgcccgaaca ccaatactgc
    22681 ggcaaaaaac agtgtaatat agtggattaa att
//
LOCUS       DAXDSD010000025        21593 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXDSD010000025 DAXDSD010000000
VERSION     DAXDSD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21593
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.025"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            61..303
                     /gene="imm31"
                     /locus_tag="ACFB2Q_000742"
     CDS             61..303
                     /gene="imm31"
                     /locus_tag="ACFB2Q_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC0917814.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            381..626
                     /locus_tag="ACFB2Q_000743"
     CDS             381..626
                     /locus_tag="ACFB2Q_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC0917815.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLIREGLHHDTGHIGWRAMSKGK"
     gene            629..1019
                     /locus_tag="ACFB2Q_000744"
                     /pseudo
     CDS             629..1019
                     /locus_tag="ACFB2Q_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1059..2516)
                     /locus_tag="ACFB2Q_000745"
     CDS             complement(1059..2516)
                     /locus_tag="ACFB2Q_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC0917816.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIS
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(2531..3361)
                     /locus_tag="ACFB2Q_000746"
     CDS             complement(2531..3361)
                     /locus_tag="ACFB2Q_000746"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC0917817.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(3438..3827)
                     /locus_tag="ACFB2Q_000747"
     CDS             complement(3438..3827)
                     /locus_tag="ACFB2Q_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC0917818.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGTVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(3974..4501)
                     /gene="nspA"
                     /locus_tag="ACFB2Q_000748"
     CDS             complement(3974..4501)
                     /gene="nspA"
                     /locus_tag="ACFB2Q_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC0917819.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYVGKVNTVK
                     NVRSGELSAGVRVKF"
     gene            complement(4944..6119)
                     /gene="hemW"
                     /locus_tag="ACFB2Q_000749"
     CDS             complement(4944..6119)
                     /gene="hemW"
                     /locus_tag="ACFB2Q_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC0917820.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGAGFIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(6176..8647)
                     /gene="ligA"
                     /locus_tag="ACFB2Q_000750"
     CDS             complement(6176..8647)
                     /gene="ligA"
                     /locus_tag="ACFB2Q_000750"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC0917821.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     SNIPLRLHGENVPELIEVRGEVLMLKADFAGLNKRQAENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSIARQQGGFEAEEHIQELAYFQELGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRIDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLC"
     gene            complement(8781..10067)
                     /locus_tag="ACFB2Q_000751"
     CDS             complement(8781..10067)
                     /locus_tag="ACFB2Q_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC0917822.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEEIGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDMRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(10261..10833)
                     /gene="ampD"
                     /locus_tag="ACFB2Q_000752"
     CDS             complement(10261..10833)
                     /gene="ampD"
                     /locus_tag="ACFB2Q_000752"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC0917823.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            10917..11912
                     /gene="mltG"
                     /locus_tag="ACFB2Q_000753"
     CDS             10917..11912
                     /gene="mltG"
                     /locus_tag="ACFB2Q_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC0917824.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            11972..12592
                     /gene="tmk"
                     /locus_tag="ACFB2Q_000754"
     CDS             11972..12592
                     /gene="tmk"
                     /locus_tag="ACFB2Q_000754"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC0917825.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            12814..14094
                     /locus_tag="ACFB2Q_000755"
     CDS             12814..14094
                     /locus_tag="ACFB2Q_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC0917826.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            14496..15527
                     /gene="lpxK"
                     /locus_tag="ACFB2Q_000756"
     CDS             14496..15527
                     /gene="lpxK"
                     /locus_tag="ACFB2Q_000756"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC0917827.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKGKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            15733..16266
                     /locus_tag="ACFB2Q_000757"
     CDS             15733..16266
                     /locus_tag="ACFB2Q_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC0917828.1"
                     /translation="MKLKTLLLPFAALALCANAFAAPPGDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGIVD"
     gene            16251..16358
                     /locus_tag="ACFB2Q_000758"
                     /pseudo
     CDS             16251..16358
                     /locus_tag="ACFB2Q_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            16488..16670
                     /locus_tag="ACFB2Q_000759"
     CDS             16488..16670
                     /locus_tag="ACFB2Q_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC0917829.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            16667..17428
                     /gene="kdsB"
                     /locus_tag="ACFB2Q_000760"
     CDS             16667..17428
                     /gene="kdsB"
                     /locus_tag="ACFB2Q_000760"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC0917830.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRVGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            17451..17966
                     /locus_tag="ACFB2Q_000761"
                     /pseudo
     CDS             17451..17966
                     /locus_tag="ACFB2Q_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            18123..18215
                     /locus_tag="ACFB2Q_000762"
     tRNA            18123..18215
                     /locus_tag="ACFB2Q_000762"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18157..18159,aa:Ser,seq:gct)
     gene            18489..19274
                     /gene="trpA"
                     /locus_tag="ACFB2Q_000763"
     CDS             18489..19274
                     /gene="trpA"
                     /locus_tag="ACFB2Q_000763"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC0917831.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIGSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVAAAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            19311..20183
                     /gene="accD"
                     /locus_tag="ACFB2Q_000764"
     CDS             19311..20183
                     /gene="accD"
                     /locus_tag="ACFB2Q_000764"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC0917832.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(20243..20854)
                     /locus_tag="ACFB2Q_000765"
     CDS             complement(20243..20854)
                     /locus_tag="ACFB2Q_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC0917833.1"
                     /translation="MRRAILLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCALSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            20962..21186
                     /locus_tag="ACFB2Q_000766"
     CDS             20962..21186
                     /locus_tag="ACFB2Q_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC0917834.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            21217..>21593
                     /locus_tag="ACFB2Q_000767"
     CDS             21217..>21593
                     /locus_tag="ACFB2Q_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917835.1"
                     /translation="MLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLFRSACVLTQGS
                     FEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYLSLTRDSCSVK
                     AERADSAALLEHFSGLVRQPPKNA"
BASE COUNT         4753 a         5231 c         6519 g         5090 t
ORIGIN      
        1 atgggctagt agggatggta agcatttaaa tatatcaatt gatggaaaga ttacacacaa
       61 atgactaatt taaaattaga tttctactct gaagttatta taaaagattc ttgccctaat
      121 gatttgttag aaaatggtga gactattaaa ggaaaaaaag gagtggtatt aggtataagc
      181 gaagaagatg gtataatcta tggctatact attttacttt ttgatataaa atattgtata
      241 tatatagata agaaatatat tataccgact ggaaaaaaat tctctcgcga tgatttttat
      301 tagctaattt aagtaattta agcaagtttt ttagatactg aaactggtaa ttatgtttat
      361 gtaagcataa ggagttcaga ttgaaggatt ctacagggca aatgaggctg gcaaccaagg
      421 atttggcgga agccattaaa cgaggagaag tacgtagttc tgcttttaca acaaagcaac
      481 taaaggcaat cgaaaaagga gaatctaaaa taccagatta cacttggcat catcatcaag
      541 atacaggaag gatgcaattg attcgtgaag gcttgcatca tgataccggc catatcggtt
      601 ggagagcgat gagtaaagga aagtaactat gtggaaaatc ataaaagagg atagtgatga
      661 tttaggattt gcaattaaat gcttattctc tcaatctatt gatttaaatg aattcaagtt
      721 atggattgaa caagtaatac gcgatatgcc catcgaggac atcccttttt atatttttga
      781 cttggcggat ttcaatgggg gaattggaga cattgacaat attgtaggtt ttgtttcgag
      841 ttacagccta tcaaaatcga aaaaaaatgc cttgacaggc attgccttct taagggggat
      901 agatgtctat agaccctccc gtttcaaaag aaaaagcatt aaaagcctta gagaaatatc
      961 ctgaaattta tcataaattt aaatggttct ttccttttgt agagcttccg cttctttaaa
     1021 ggacaatatg ccgtctgaaa agttttcaga cggcattttt atttcttcca gtaggcgggg
     1081 gtgaagagga tgaagacggt gaagatttcc agcctgccca agagcattgc ggtaacgcag
     1141 atccatttct gcatcacgtc caaaccggcg taattgccgg cgggcccgac ttcgcccagt
     1201 ccggggccgg cgttggtaat gcaggcgatg acggcggtaa aggcggtggt aaattccata
     1261 ccgctcgcca tcagcaggaa gctgaagagg acgacggtca taaagtagat gaagatgaag
     1321 gacataacgg tcagcgcgag gcggtcgggt atggctttgc cgctgatttt gacggtgcgg
     1381 acggctttgg ggtgcagcag caccatcatt tcgcgcaggc tgaatttgaa caggacgagg
     1441 gcgcgtatgg ttttgatgcc gccgccggtc gagccggagc tggcgaggat gttggcgagg
     1501 aaaaacatcc acagggaaat caggagcggc cattgtgcga agtcggtgtt ggacagcccg
     1561 tttgccaatc cgatggagac gaagttgaag gcggtgtagc gcagggattc gccgaagccg
     1621 gcgtaatggc cggtgtgcca caggtacagg gcggcggcaa ggatgctgcc ggagagcagc
     1681 agcagcatcg tccggcattc ttcgtctttc caataggttt tgaggctgcg gctgttgagg
     1741 gcggcgaaat ggctggcaaa attgatgccg ccgaaaatag tgaaaacgat gatgactgct
     1801 tcgatgaggg gggagttgta ataaccgatg ctggcatcgt gggtggaaaa cccgcccagc
     1861 gagagggttg ccagcgcgtg acagacggca tcgaaccaac ccatcccggc aaaatgcagg
     1921 caggctgccg cgaggatggt gatcagggtg tagccgaacc agagtttttt cgccacttgg
     1981 gaaatgcgcg gcgacatttt gctttctttg tcaatgccgg ggatttcggc tttgaataac
     2041 tgcgtgccgc ctacgccgag cataggcagg atggcgacgg caaggacgat gatgcccatc
     2101 ccgcccagcc agttgagcat atgccgccaa aagttgacgg agggggcgag cccgtcgacg
     2161 tgggggatga cggtcgcgcc ggtggtggtc agtcccgaca tcgattcaaa aaatgcgtcg
     2221 gtaaagccca tattcgggaa atacaggtac atcggcatcg cagccatagc ggcaaacgcc
     2281 agccacaaca tcaggacgag ggtgaagccg tcgcgcgggc gcagttcgcg cctgaaccgg
     2341 agggtggcga gccggacgat gcacgagccg gaaagggtaa cggtcgcggt ggtggcgaat
     2401 gcggtgtacg cgccgtccga aaaggcgtag gaaagggcgg cgggtatcag caggataaag
     2461 gaaaacagca tacccagtcg ggagaggacg tgggcgatgg gcaggatttt gtgcatagtg
     2521 gggcggtccg ttattttgcg aagcttttcc agtctatgcc gccggcggct tggacttggg
     2581 taatttcttc cgtttcgagg ttgacggcgg tcagctgccc gccccacagc gcgccggtgt
     2641 ccagcgagat gacgttgtcg gcattcgtgt agcccagcga ggaccagtgt ccgaagatga
     2701 tggtgtggtc gaggttttgc cgatcggggg ctttgaacca cgggcgcagg taaggcggca
     2761 tttttttcac tgtggatttg tagtcgaaat ccagttcgtt tttaaaggtc agggcgcgca
     2821 tccgcgtgaa ggcgttgacg atgaagcgca ggcgggcata gccttccaaa ccttcgtccc
     2881 atgcggccgg tttgttgccg tacattttgg agaagaattt gacgtatttt ttgccgcgca
     2941 gttcggcttc ggcttctccg gcgagcgatt cggctttggc tatgcgccat tgcggcagga
     3001 tgccggcgtg tatcatcacg cggccgccct cgcgtatcaa aagcggttgc gcgcgcagcc
     3061 agtcgagcat ttttccgccg tcggggtgtt tgagtatggg ttcgattgtg tcgctgcgtt
     3121 tgagcgcgcc ttcgccgcag ccgacggcga gcaggtacag gtcgtggttg ccgaggacga
     3181 tttgcacgct gttttcgtgc cggatgcaga attgcagcgt ttcgagggat ttcgggccgc
     3241 ggttgacgat gtcgcccgtc agccagaggg tgtccgtgcc gtggttgaag ccgattttgc
     3301 cgagcagcgc ggtcagttcg tcgaaacagc cttggatgtc gccgattgcg taatgtgcca
     3361 ttgcgggtgt tgtgaagtgg gaaagtgttg cggttcggac ggcacggttg gaaatcttat
     3421 gccgtccgaa cgtggaatta tgcgttcaaa acgaggacgg cttccgcttc gacctgcacg
     3481 cctttgggca gcgaggcaac gccgacggcg gcgcgggcgg ggaacggctc ggtgataaat
     3541 tccgccatca cttcgttgaa gacggcaaaa ttgcccaagt cggtcaggta ggcgttgagt
     3601 ttgacgatgt cgtccagcga accgcctgcc gcttcggcga cggcctgcag gttttggaac
     3661 acttggcgcg cttcggtgtg gaaatcgccg ttgccgacca cagtcatcgt ggcgggatcg
     3721 agcggaatct gaccgctcat gtaaacggtg ccgccggcgc gcacggcctg gctgtatgcg
     3781 ccgatggcgg cgggggcttt gtcggtatgg atgacggttt tggacatttc ggattcctca
     3841 aaaaataggg cggcagaagc cgcagcgttc gggattatcg tacaaaaccg ccggcttgtg
     3901 tagttttagg cggtatatgt gccggaaaca aaaaaccgcc gaaggctcgg cggtttgcgg
     3961 aataacgcgt atatcagaat ttgacgcgca cgccggcgga cagttcgccg gaacggacgt
     4021 ttttgacagt gttgactttg ccgacgtagt tgtagcggta gccggcatcc agatcgacat
     4081 tgggtgtaac ggcatagctt acgcccgcca atacgccgag gccggcggag gttttgctga
     4141 agctgtcgct gccgcccaag tgggcggaag cgcggttgag gctcaagcgc gcgccgaaat
     4201 acggtttgac gggcgattgg gtgtcgaagt cgtaaatgac ggacgcgccg atgctgtaaa
     4261 gtttgaaatc ggtggatggg gcttgtttat agtttttgta gcgcgtgtaa tcgacggcga
     4321 agcggaggtc gttgatgcgg tagcctgcgg agatgcgcgg gctgaagcct ttggcagaac
     4381 ctaaagagct tgaggctttg gcgtgtgcgg catcggcttg gacgtaaaag ccggatgcgc
     4441 cttccgccag tgcggcggcc gggagtgcga gggcaatcag tgcggcaagt gcttttttca
     4501 tattttggtt cctttatggt cagggagaaa aattgttaat aatccgttaa agaatcctgc
     4561 cgtattatac tgaaattttc tttttgcatc gtaatatttt caatgcctca agatacgcgg
     4621 cggcatccgg ctgctttgcc gacggcaaag ccgttaaccc gcgcgttgcc cttaaacggc
     4681 ggcatcacgc ggcggatggg tgaaacttgc aaacggtttg gaaaaaaccg caaaggcggg
     4741 gcggcggcat ttgtcagatt gttgcaggcg caggcgtacg gttttttgtg tgcggcgtta
     4801 ccttaggcgt cggacatttc cggcggcggc tgtgccgtct gaaacgcccg gcgggggatg
     4861 cggctgcgtt tcccatcgat aggcatattt gccggccgcg ttcggggcgg gttttacccg
     4921 ggcggccgcc gatttgtttg cgcttataaa aaacactgca acaaatcgtt taaaaacaag
     4981 cgtccttttt cggtcgggcg gaatacggtc gggtcggttt ccagcaggcc tttttgcctt
     5041 gccgtttcga tttgcaccat gattttggcg gcgggtacgc ccgtgcgctc ctgcaacatc
     5101 gcggcgggta cgccgtcggt caggcgcagg gcgttcatca tgaattcgaa cggcaaatct
     5161 tcggcggcga cggttttgcg ttcgacggct tcgcccggct gactttgcat taaggcgagg
     5221 tagtcgttgg ggtggcggcg gcggacggtg cgctcgatgc ggtcgggata ggagattttg
     5281 ccgtgcgcgc ccgcgcctat gcccaaataa tcgccgaact gccagtagtt caaattgtgg
     5341 cggcactgca tggtgggttt cgcaaaagcc gatgtttcgt agtggataaa acccgcgcct
     5401 tccagtgtgc cgtgtaccgc gtcttcgatg tcgagcgccg cttcgtcttg cggcaaacct
     5461 ttcggcggcg tatgaccgaa cggcgtgttc ggctccatcg tcagatgata tgcgctgatg
     5521 tgggttgcgt ctgtggcgat ggcggtttgc acgtcgtcca atgccgtctg aacggtttgg
     5581 ttcggcaggg agtacatcaa gtcgatattg actttatcaa ataattttaa ggcggtatcg
     5641 atagcggtta aggcttcttt gccattgtgg acgcgcccca gccttgagag catatcgtcg
     5701 ttgaaactct gtacgccgat agaaagccgc gtgatgcctg cgtctttaaa tccttgaaac
     5761 ttctcgattt caaatgtacc cggattggct tccaacgtaa tttccgcttc gggctgcaag
     5821 cgcaacagcg aacgcacgcc gcttaacaaa cggtcaatcg attccgcctg aaacaggctg
     5881 ggcgtaccgc cgccgaaaaa tatcgtttcc accggcctgc cccaaatatt gggcaattca
     5941 agctgcaagt cggtcagcag cgcgtcgata taggcggctt cgggcaatcc gtttttcagg
     6001 ctgtgggaat tgaagtcgca atacgggcat tttttgatgc accacgggat gtggatatag
     6061 agcgacaggg gcggcagggc ggtgagtcgg gtgcggtttg gaaaggaaat ggtgtgcatg
     6121 gtgcggttcg gaaaagtgga cgatgccgtc tgaaggcggt tcggacggca tgggttcagc
     6181 agagcagggt aagcagttcg gcttcgctga ggacggaaac gcccaaggca ttggcttttt
     6241 ccagcttgct gcccgcggct tctccggcga cgacgtagtc ggtttttttg gacacgctgc
     6301 cggacacttt gccgcctgcg gcttcgatta gggcttgggc ttggtcgcgt ttgaaggtgg
     6361 gcagggtgcc ggttaacaca aaggttttgc ctgccacggc tttattgatg ccgtctgaac
     6421 cttgcgccgc ctcgtcttca gacggcattt gcgcgaagaa ggtttttagg ttttcgagca
     6481 gggcggtgtt ttgcgctttg ctgcgccacg cctgccagtc ggcagggagg gctttgtcgt
     6541 tttgcagtcc ttctatactt tgtccggcga gttcccataa ggcttgggct ttgttttcgc
     6601 tgattttgaa accgggcagg cgggtaatcc agcgttgcgg cccggcatat tgtgcggcag
     6661 gcaggctgac ggcttgggct tgcggggcaa cgcctgcggc gagcagctcg tctatcatcg
     6721 cctgctgttc ggcttgggcg aagaagtggg caatggaacg cgccactacc gtgccgatgt
     6781 cgggcaggca ggcgaggacg ggttcggggg cgcggcggac gcgttccaat gtgccgaatg
     6841 cctgcgccag cgttttggcg gtgcgttcgc cgacgtggcg gatgccgagc gcgaacagga
     6901 agcgggcaag ttcgggcgtt ttgccggatt ctatgcctgc gaggatgttt tgcgcccact
     6961 tggtcggttg ttttttgccg ttttgggtat tgtatttaga caggtcgcct gaaaccgttt
     7021 ctgcgtcgcc gttttcgttt tcagatgacc ctttatccgc cgtttccttc attttttgca
     7081 gggtcggaat atcgatgcgg tagaggtcgg cgaaatggcg gacgaggtct tgggcgacca
     7141 gctgttcgat ttgtttttca cccaagccgt cgatgtccat cgctttgcgc gaggcgaagt
     7201 ggattaagcc ttgcgcgcgt tgtgcctgac aaagcatacc gccgctgcat cgggcgacgg
     7261 cttcgccttc ttcgcgttcg atttcgctgc ggcagatggg gcagcgggcc ggcaggcggt
     7321 agggcttatg gagcggaacg gattcggttt gcttggcgga cggtgtttcg gcaaacaggt
     7381 cgtcctgctg atgcccgatg ccgtctgaaa cggcaacggc ggtttcctgc atcgggcggc
     7441 gttcgaaaat cacgcgcacg acttcgggaa tcacgtctcc ggcgcggcgc acgacgacgg
     7501 tatcgccgac gcgcacgtct ttgcgcgata cttcgtcctg attgtgcagg gtggcgttgg
     7561 taacggttac gccgccgacg aatacgggtt gcaggcgggc aaccggcgtt accgcgcccg
     7621 tcctgccgat ttgcacgtca atcgcttcga caatggtcag ggcttcttcg gcagggaatt
     7681 tgtgggcaac cgcccaacgc ggcgcgcggg agatgaagcc gagttcgcgc tgttgcgcca
     7741 agctgttgac tttgaccacc atgccgtcga tttcgtaggg cagttcgggg cgtttttgct
     7801 gcatgtgttc gtaaaacgcc aatacttcgc cgatattttt gaaacagccg aaattgccat
     7861 tgggcagact gaagccgagt tcttggaaat aggcgagttc ttggatgtgt tcttcagctt
     7921 cgaaaccgcc ttgctgacgg gcaatcgaat aggggaaaaa gtgcagtttg cgttgcgcgg
     7981 tgatgcgcga atcgagttgg cgcaggctgc cggcggcggc gttgcgcgga ttggcaaagg
     8041 gtttttgccc gttttcggct tggcgtttat tgagcccggc aaagtcggct ttgagcatca
     8101 gcacttcgcc gcgtacctcg atgagttcgg gcacgttttc gccgtgcagc cgcaggggga
     8161 tgttggatac ggttttgacg tttcgggtaa cgtcttcgcc cgtcgtgccg tcgccgcgcg
     8221 ttgccgcctg caccaatacg ccgtcgcggt agagcaggct gatggcgagg ccgtcgaatt
     8281 tgggttcgat aacgtattcg ggattgccgc cgtccaagcc gtcgcgcacg cgttggtcga
     8341 aggcgtacat ttcggcatgg tcgaacacgc cgttttcatc ttgcggggaa aaggcgttgg
     8401 tcagcgacag catcggcact tcgtggcgca cttcggcaaa gcctgccaaa ggctcgccgc
     8461 cgacgcgctg ggtcgggctg tcgggcagct tgagctcggg atggtttcgt tccaacgctt
     8521 cgagttcgcg gaacaggcgg tcgtattcgg catcgggtat gctgggcgcg tcgagggtgt
     8581 agtattcgta ggcgtagcgg ttgaggaggt cggtgagttc gtgtatgcgt tgtgcggtcg
     8641 ggttcatgga ttcgtacagg ttcagacggc atggacaaat gccgtctgaa cggtaaaacg
     8701 tgttggaaat ggtcggattt tacctgaaaa caaaaggcgg atgcaccgtt gccgatgtat
     8761 ccgcctttgt gtttgcggag ttatgaaaac aggcgcaggg cggttttgcc gcccggttcg
     8821 ataccgactt tgagcatctc ggactgacgc gccaatacat aagtgcgtac gtctttgagc
     8881 cattgggtcg aaacttcttc cattttgtcg ttgaccagat tcaggttcaa ctgaccggac
     8941 aggcgtaccg ccaaatccat aaacaaatcg tcgaaggttt tttcgcctgc cggagagtgc
     9001 gggatgtcga gcagcatact gaagcctttg taggactggt tgtccaaaag ggcattggta
     9061 aacggctcgt tgttgagcga gcagatggag aacatggtcg agcccgacgt gtcggtatag
     9121 tggaacgcgc cgtcgtcttc caaaacgaaa cccacgcccg ttacggcgga acgcagttct
     9181 acgccgctga tgctggtcgg cgaaaccaaa tggatggcga tggtctggtc gacgcgcgcg
     9241 cagaatgcgt ccagtgcgga agccacttcg ataaaggcgg caaggtcggt gtgcagcgtc
     9301 tgaccgccca tgctttgtgc gaatgcgtcc gcctggcggt tgaatgcgga gagttcttcc
     9361 tgcgaggcaa gtccgttgcg gctgactgcc tggataccca cgataaatgc ctgatagcgg
     9421 atgcccggga tgggttcggc aatctggaaa tggtcgtcca tggtgcagcc gacaatctgg
     9481 tagcggcagc ggttggaaag gcgcggcagt gcgtgcagtt ctttggcttc ggtcagcgcg
     9541 atataggaga tgaagtcgaa gcgcatgtca aaccagggca gctcgacctt cgacagctct
     9601 ttgagcgtaa tcagcggttt tgccggcgtt tgcggaacgg gaacgggttt tgccggcgcg
     9661 tcggcaggtt tcggtgcgga atgtccggtt tggggttcgg aaacggtgtg ggcggagttg
     9721 ccgataatgc cgatttcttc caaggcggtt tcgatttcgg ttttgaacgg ggaggctttt
     9781 gcctgtttct gcttggcgat gtagacggca tcctgttctt gcaggttgcg catggcggag
     9841 tcttggggtt tggccggttt tttgaccgcc ggttggggtt tcggcatcat gactggcccg
     9901 ccggacggtt tgccgtcgcg gacatggctg gttttgctgt tgagcagggc atctttgtcg
     9961 gagtgtccga actggtcgcg cacttttttg cggtattggt tttcctgata catattgtag
    10021 gcgacaacgg cgaggacggc ggcgaggaac agtacgatgt aaatcatggc aatcacttta
    10081 aatttcggga tgcaggatgc gcaaagtgcg ggtactgcgg ttaaatcggg cttgcgtttc
    10141 cggcagtctg acggaacggc cggttataac gtttgaatta taacgaaaat tgcagggtct
    10201 gacagcagtg tgtcgaaata agcggaaatt ttccgaaatg ccgtctgaaa tctgtggttt
    10261 tcagacggca tttctgtcca cgggaaaccc tttttcccgt atccgccgcc agtcgaaaaa
    10321 atggccgggg tcggttttgc ggccgggtgc gatgtcttgg tgtcccgtta ccgccgtgat
    10381 ggggtagcgg cggcagaggg ctgccaacag cgtttcgagc gaacggtatt gcgcttcggc
    10441 aaacggttcg aaatcgcagc cttccagttc gatgccgatg gaaaacgcgt tgcacttctc
    10501 ccgcccccga aacgaggatg cgccggcgtg gtacgccatg tcgccgcatg atacgaactg
    10561 caccgtttcg ccgtcgcgtg caatcaggaa atggctggac acgtgcaaag tgtgtatcag
    10621 gctgaagaac ggatgtccgt cggggtcgag ccggttggca aacagctttt ccaccgcatc
    10681 cgtgccgtat tcgaacggcg gcagcgaaat gttgtgcagc acgatcaggg aaaccgtttc
    10741 tcccgtttcc ctcgggctga aattgggcga cggggtatgg cgtatgcttt gaagccagcc
    10801 gttttgccag tgtgctccgg cgtgattgtc catgatgttc ttcctgtccg gcgggcaatt
    10861 tgggttatac tgtcgcccga attttaagac gtattccgaa tgctgggaat cctaccatgt
    10921 tgaaaaaaat gttgaaatgg actgccgttt ttttgaccgt atcggcagcc gttttcgccg
    10981 cgctgctttt tgtccctaaa gacaacggca gggcataccg aatcaagatt gccaaaaatc
    11041 agggtatttc gtcggtcggc aggaaacttg ccgaagaccg catcgtgttc agcaggcatg
    11101 ttttgacggc ggcggcctac gttttgggtg tgcacaacag gctgcatacg gggacgtaca
    11161 gactgccttc ggaagtgtct gcttgggata tcttgcagaa aatgcgcggc ggcaggccgg
    11221 attccgttac cgtgcagatt atcgaaggtt cgcgtttttc gcatatgagg aaagtcatcg
    11281 acgcaacgcc cgacatcgga cacgacacca aaggctggag caatgaaaaa ctgatggcgg
    11341 aagttgcgcc cgatgccttc agcggcaatc ctgaagggca gttttttccc gacagctacg
    11401 aaatcgatgc gggcggcagc gatttgcaga tttaccaaac cgcctacaag gcgatgcaac
    11461 gccgcctgaa cgaggcatgg gcaggcaggc aggacgggct gccttataaa aacccttatg
    11521 aaatgctgat tatggcgagc ctgatcgaaa aggaaacggg gcatgaggcc gaccgcgacc
    11581 atgtcgcttc cgtcttcgtc aaccgcctga aaatcggtat gcgcctgcaa accgacccgt
    11641 ccgtgattta cggcatgggt gcggcataca agggcaaaat ccgtaaagcc gacctgcgcc
    11701 gcgacacgcc gtacaacacc tatacgcgcg gcggcctgcc gccaaccccg attgcgctgc
    11761 ccggcaaggc ggcactcgat gccgccgccc acccgtccgg cgaaaaatac ctgtatttcg
    11821 tgtccaaaat ggacggcacg ggcttgagcc agttcagcca tgatttgacc gaacacaacg
    11881 ccgccgtccg caaatatatt ttgaaaaaat aaaccatgcc gtctgaaaag tttgtgtttt
    11941 cagacggcat acccttaccg gaactgcaag catgaaaccg caattcatca ctttggacgg
    12001 catagacggt gccggaaaat ccaccaacct tgccgtcatc aaggcatggt ttgaacggag
    12061 ggggctgccc gtgctgttca cgcgcgagcc gggcggaacg ccggtcggtg aggccttgcg
    12121 cgaaatcctg ctcaaccctg aaaccaaagc cggtttgcgt gcggaaacac tgatgatgtt
    12181 cgccgcgcgt atgcagcaca tcgaggaagt catcctgccc gcgctttcag acggcatcca
    12241 tgtcgtgtcc gaccgtttta ccgatgcgac cttcgcctat cagggcggcg ggcgggggat
    12301 gccgtctgaa gacattgaaa ttttggaaca ttgggtgcag ggcggtttgc gccccgattt
    12361 gaccctgctg ttggatgtgc cgctggaagt atcgatggcg cgtatcggac aggcgcgcga
    12421 gaaagaccgg ttcgagcagg agcaggcgga tttctttatg cgtgtgcgcg gcgtttatct
    12481 cgaccgagcc gccgcctgtc ccgaacggta cgccgttatc gacagtaacc gcagcttgga
    12541 tgaagtcaga aacagcatag aaaaagtgtt ggacggacat ttcggctgtt gatgcggcaa
    12601 atattgaaac aagcgcatcc gcccgcgccg aaaaccaaac ggcagtgccg caggtgaaaa
    12661 tggcggtatg cgccaaactt tcggcatgat agaattacgc tcggttacaa ggcaggatgc
    12721 gtcggcaata ttaacgaacc gcccgtaaca tgatgacccg aaagcgtttc ggacagttcg
    12781 attcaaatct ttttctcgca acaggattga cacatggaaa actcattgaa agaagccgcc
    12841 ctcaagttcc acgaattacc cgttccgggc aaaatttccg ttaccccgac caaatctctg
    12901 gcgaccgaca aagatttggc gttggcgtac tctccgggcg tagccgcccc ctgtatggaa
    12961 atccatgccg atccgcaaaa tgcctacaaa tacaccgcca aaggcaactt ggtcgccgtc
    13021 atttccaacg gtacggccgt tttgggcttg ggcgacatcg gcgcgctggc gggcaaaccc
    13081 gtgatggaag gcaaaggcgt attgttcaaa aaattcgccg gtgtggacgt gttcgacatc
    13141 gaaatcgacg aaaaagaccc gcaaaaactg gtggacatca tcgccgcttt ggagccgact
    13201 ttcggcggca tcaacctcga agacatcaaa gcacccgagt gtttctacat cgaacgcgaa
    13261 ttacgcaaac gctgcaaaat ccccgtattt catgatgacc agcacggcac ggccatcatt
    13321 accgccgccg ccgtattgaa cgccctgcgt tataccggcc gtaaaatcga agaagcgact
    13381 ttggtgtgct ccggcgcagg tgccgccgcg attgcctgcc tgaaccaact gctggatttg
    13441 ggcttgaaac gtgaaaacgt aaccgtttgc gactccaaag gcgtgattta ccaaacccgc
    13501 gaagacaaag accgcatgga cgaatccaaa aaattctacg ccgttgaaga caacggccgg
    13561 cgcgtacttg ccgatgcggt taaaggcaaa gacatcttct tgggcctctc cggcgcaaac
    13621 ctgctgacgc ctgaaatgtt gaacacgatg aacgaaaaac ccatcgtgtt cgccatggcc
    13681 aacccgaatc cggaaatcct gccgccgctg gcgaaagaaa cccgtccgga cgtggttatc
    13741 ggtaccggcc gctccgactt cccgaaccaa gtgaacaacg tattgtgctt cccgttcatc
    13801 ttccggggcg cgttggatgt cggcgcaacc accatcaacg aagaaatgaa acgcgcctgc
    13861 gtgtatgctt tggcagattt ggcgatggaa gaagtaaccg aagaagtggt tgccgcttac
    13921 ggtaagaaat tcgaattcgg cgcggaatac ctgattccta ctccgttcga ttcccgcctg
    13981 ctgcctcgcg tcgctacggc tgccgccaaa gcagcgatgg aaagcggcgt ggcaacccgt
    14041 ccgattgcag atttggaagc ttacgctgcc aagttgggcg aatggaagct gtaagccgtt
    14101 tgtggttaaa atgccgtctg aactgttttc aggcggcatt ttgttgtcag attgataaat
    14161 gaaagatact ggaaaatgaa agagatgaaa cctgtccgtt atcatattgg cgatataccc
    14221 gaaacttcaa aacaaaccgc ccccggtcat gacgacaggg cagtgggtgt tgacgatgat
    14281 tgttttcatg attcctttgg tcaatatcgt tatgtttttt gtttgggcgt tcggcagagg
    14341 caaccccaac cgtgccaatt tctgtaaagc actgttctta tttaccttgt tggttcgctt
    14401 atcggtttga ttttcatatt gcttataggt gggtctgcat cgggtacgca ttattaatgt
    14461 gccggctgat tctgcttcga agatttgtat cgaatatgcc aaattttttt aaatttcata
    14521 ccgttatcga acggcattgg caaaaacctt atccggtttt gtcttttctg ctcaagccgc
    14581 tctccgggct gtttgccaaa attgcggcaa aatggcgggc ggatttttta tcgggaaaac
    14641 ggcaaagcga aaagctgtcc gtgcctgtgg tcgtggtcgg caacatccac gcgggcggga
    14701 cgggcaaaac gccgattgcc gccgcgctgg tgtcgggctt gcaggaaaaa ggtgtcaagg
    14761 tcggcatcat cagccgaggc tacgggcgca agggcaaggc ggtttatgta ttgaatgccg
    14821 cgagccgtgc ggaagatgcg ggcgacgagc ctttgctgct gttccgtaaa acaggtgcgc
    14881 cgacggcagt gggcagcagc cgtgtggagg caggcagggc gttgctggca gcgcatccgg
    14941 agcttgaatt gattgtggcg gacgacggtt tgcagcatta cgccctgcaa cgcgatgtgg
    15001 aaatcgcggt gtttccggcg gcggatacgg ggcgtacgga tttggacttg ctgcccaacg
    15061 gcaatttgcg cgaacctttg tcgagattgg aaagcgtgga tgcggtggtg gtcggcggca
    15121 gggcggcgga tggttttatg ccgtctgaac atttgttcgg cagccgtatt gaggcgggtg
    15181 cggtttaccg tttgaaccgt ccgtctgaaa aactggatat ttcgacattg tcagggaagc
    15241 gcgtcgcagc ggttgccggt atcgccaggc cgcagcgttt cttcgatact ctgacacaca
    15301 tgggcatccg ccttgaccaa acggttgctc tgcccgacca tgccgatatt ttcaatcggg
    15361 atttgccgcc tgccgatgtg gtgctggtaa ctgaaaaaga tgcggtcaaa ttttcagacg
    15421 gcatttgcac cgataatgtt tgggtgctgc cggtttgtgc gataatcgaa cctgatttgg
    15481 cggagtttgt gctggagcgg ttggagggtg taccgaaggc cgtctgaaag cgcggtttgg
    15541 acgaagtgat aacggactgg aataagaacg ccctacgccg tcattcccgc gcaggcggga
    15601 atctaagtct cgaattttca ggaatgccta ggagacttca gaaatcccaa atctccggat
    15661 ttccacttgg gcaggaatga gaaagccggt tgtatttttt atctgcatta atcattcgtt
    15721 aaaggagtgg atatgaagct gaaaaccttg ttattgccct tcgccgcact ggcattgtgt
    15781 gccaacgcat ttgccgcccc gcccggcgac gcgtcgttgg cacgttggct ggatacgcag
    15841 aatttcgacc gggatataga aaaaaatatg attgaaggct ttaatgccgg atttaaaccg
    15901 tatgcggaca aagcccttgc cgaaatgccg gaagcgaaaa aagatcaggc ggcagaagcc
    15961 tttaatcgtt atcgtgagaa tgttttgaaa gatttgatta cgcccgaagt gaaacaggct
    16021 gtccgcaata ccttattgaa gaatgcccgt gaaatataca cgcaagaaga aattgacggc
    16081 atgattgcct tttacggttc gcctgtcggt cagtccgtcg ttgccaaaaa tccgcgctta
    16141 atcaagaaat cgatgagtga aatagcggta tcttggactg cattgtcagg gaaaatcgcg
    16201 cgacatcatc tgcccgagtt tacggaagag ttacggcgca tcatctgcgg cggtatagtg
    16261 gattaaattt aaatcaggac aaggcgacga agccgcagac cgtacaaata gtacggcaag
    16321 gcgaggcaac gccgtaccgg tttaaattta atccactata aaaatcccga tgcgggctgc
    16381 aagcaggccg gacagcttgg gaaaaggtat cagaagtaaa taatagccgc ctgaaatatt
    16441 gcggagggcg tccgattgat taaaccatca aacccgaaag caaccttatg gaaaaaaaat
    16501 tcttagacat cctcgtctgc cccgttacca aaggcaggct ggaatatcat caggacaaac
    16561 aggaattgtg gagccgtcag gcgaagcttg cctatccgat taaagacggc attccctata
    16621 tgctggaaaa cgaagcacga gcgttgagcg aagaggaact caaagcatga ccgaattcgt
    16681 cgtattgatt ccggcgcggc tggattcgtc gcgcctgccc ggaaaagcct tggcggacat
    16741 tcacggcaaa ccgatggtcg tgcgcgttgc cgaacaggcg gcaaaaagca aagccgcgcg
    16801 cgtcgtcgtc gccaccgatc atcccgacat tcagacggcc tgtcaggcgc acggtatcga
    16861 agtcgtcatg acttcaaacc ggcacgaaag cggcacgacg cgccttgccg aagccgccgc
    16921 cgcgctgaag ctgccgccgc atttgattgt tgtgaacgta cagggcgacg agccgctgat
    16981 tgcccccgaa ctcatcgacc gcaccgccga agtactcgtc gaaaacaacg tccaaatggc
    17041 gaccgccggc cacgaattgc acgatttcga cgaattgatg aatcccaatg ccgtcaaagt
    17101 cgtcctcgac aaaaacggca acgccatcta cttcagccgt gccccgattc cctatccgcg
    17161 cgatgcgatg cgtgccggaa aacgcgaaat gccgtctgaa accgccgtcc tacgccatat
    17221 cggcatttac gcctaccgcg tcggcttcct gcaacgctat gccgaaatga gcgtctcgcc
    17281 gctggaaacc atcgaatcgc tggaacaact gcgcgtcctg tggcacggct accccatcgc
    17341 cgtcgaaacc gccaaagaag cccccgccgc cggtgtggat acgcaggaag atttggacag
    17401 ggttcgcgct gtatttcaga ccgtataaaa caggttcaaa gggaaaagat atgcagcaac
    17461 atattgaaaa gtggcaacac ttgagccgtg aagaacagaa aatccttgcc gaagtatggg
    17521 gtctcgtgca aaacgatgat caggaggttc actatgaaat gctcaaattg aacgcacctg
    17581 atgaagtcag cggtgaattt tggttcagaa tggcagaaac actcagcacc ctgccgccca
    17641 atcgttccct cgaccttaga atgaacggcg gcaggctgtc gaccgccgta tccatccttt
    17701 ccgtcatgat tgaagacaat cccgacatac cgcagctttg ggcgtaaaaa attaccgccc
    17761 tcaattattt ggtacacgga cacaaagccc gtgccgacgg tttggtacaa cagcccgaca
    17821 aagcggcaga agccaacgag gaggaatacc tgaccaaagc cctgtcgcaa aacctgctgt
    17881 caacattgga tgccgcactt gcacgttttc ctgaagacgc gtggtttcag gaaatcaaac
    17941 aggatgcaca aaagcatttt gcttgaggac gtggcggtca ggaatatttc cattcaggaa
    18001 gaaaagaagt gcctaattgg gtataatcag ggtaaatctt attttatttc agaagattaa
    18061 tatttgtttt ccgtttttcc ttgacggtat cggaaaagtt gattatagtt acagcttcct
    18121 taggagtaat ggctgagagg ctgaaggcac ttccctgcta aggaagcatg tgggatcaac
    18181 ctgcatcgag ggttcgaatc cctcttactc cgccagataa aaaaatagac gctgtgtttt
    18241 acagcgtcta tttttttatg caattttata gcgggttggc gcaaacccgg tatggtattg
    18301 ccctgtcttg attctgaatt ttgttaatcc gagatgtttg ccgtttattt ttgcctcgtt
    18361 caaacggcgg ctctgatctg cgcggtttct gtttgccgta ttcgcctatc cgtaccgcaa
    18421 atgttatact gggaaaaatt caccgattgt attttacggc gtatttgccg ataggatgga
    18481 agagacaaat gagcagaatc cggcaggctt ttgccgcttt ggatggcgga aaggcattga
    18541 ttccctatat tgcggtgggc gaccccgata ttcggacaac tttggcattg atgcacggca
    18601 tggttgcaag cggtgcggat attttggagt tgggcgtgcc gttttccgat ccgatggcgg
    18661 acgggccggt tattcagcgt gcggcggagc gggcgttggc aaacgggatt tcgctgcgcg
    18721 atgtcttgga tgtcgtcaga aaattccgtg aaaccgacac gcaaacgccg gttgttttga
    18781 tgggatattt gaatcccata cataagatgg gttatcggga gtttgctcag gaagccgcaa
    18841 aggcgggggt ggacggcgtg ttgacggtgg attcccccat cgaaaccatc ggttctttat
    18901 atcgcgagct gaaggataac gaggtcgact gtattttcct gattgcgccg acgacgacgg
    18961 aagaccgtat taaaaccatt gccgagctgg caggcggatt tgtctattat gtttcgctca
    19021 agggcgtaac gggcgcggca agtttggata cggatgaggt ttcgcgtaaa atagagtatt
    19081 tgcgtcagta tatcgatatt cccatcggtg tcggtttcgg catcagcaat gcggaaagcg
    19141 cgcgcaaaat cggccgggtt gccgccgcga tcattgtcgg cagccggatt gtgaaagaga
    19201 ttgaaaacaa tgcgggcaac gaggctgccg ccgtcggtgc tttggtcaaa gagttaaagg
    19261 atgccgtgcg ctgattctgt cgcgcattct aaatgtttta ggagttgtcc atgagctggt
    19321 tagataaaat cctgccaccc aaaatcaaga atcgtgggaa agacggttct tccaatgttc
    19381 ccgagggttt gtggcgcaaa tgcccgtctt gttcggcaac cgtttattcg accgagttgc
    19441 agcagaacaa tcaggtctgc ccgaaatgca accaccacaa tccgttatcg gcgcgccagc
    19501 gtttgaacct gcttttggat gaggaaggca gggaggaagt tgcgggtaat gtcaaaccga
    19561 ccgatccttt gaagtttaaa gacagcaaaa aatatccgga ccgtttgagt gcggcacgca
    19621 agctgaccgg ggaagatgac gcgctggtgg tgatgaaggg gatgatgaac ggtctgcccg
    19681 tcgtcgttgc cgcgtttgaa ttccgcttta tcggcggttc gatgggttcg gttgtgggcg
    19741 agcgtttcgt acagggtgtc cgccgtgcgg ttgccgataa ttgtccgttt gtctgtgtgg
    19801 cggcttccgg cggcgcgcgt atgcaggagg gtgtaaactc gctgatgcag atgacgaaaa
    19861 ccagtgccgc gctgcatttg ctgacggaaa aacgcctgcc gtttatatcg gtgttgaccg
    19921 atccgactat gggcggcgta tctgccagct tcgcattttt aggcgatgtc gtgcttgccg
    19981 aaccgaacgc gctgatcggt tttgccggtc cgcgcgtgat tgagcagacg gtgcgcgaaa
    20041 cgctgccgga aggcttccaa cgcgccgagt ttctgctgga aaaaggcgcg atcgaccaga
    20101 ttgtcgaccg ccgcgatatg aagcggcgca tcagtgattt gattacgctg ttgtgccgtc
    20161 aggacaaagt ttccgccacc tgatggctga tgaatcgaat gccgtctgaa accgatgttt
    20221 cagacggcat ttttgtgtct ggttatttgt cgtgcggctt catcgacggg gcatagcgtc
    20281 cggcacgttc tttcaggcgt tgtaccaaac cttgcgtgtc ggcgggtacg ccgccctcgc
    20341 agaatgcctg atacaggacg gcgcgcaatg cgtcgttgcg cccaagtgtg ccgcctatcg
    20401 gtttccattc ggcgtttcgg ggctgtatcc agcggctgtt gaccgtatcg ccgtatccga
    20461 atactttgta ggaggaaagt ttgccgttgc cgaacccgat ggaggagagg gcgcagaata
    20521 tgccttcggc agtcaggttg tcgtagcctt tgtcggagcg gatattgaga atgtagcgga
    20581 tgctgccgtc gggcgcgggc ataatttgca ggctgtcgag caggattttc ggctgtttgc
    20641 cgtaattttc atccacataa atgtcaaacc agccgtccga gtgcgcatcg ggcagaggcg
    20701 gcagttcggc ggtatgttct ttaaattcgc gggcggcggc ttcttcgggc gtttcgcggt
    20761 agcgggtgtt gatcggcgtg tctttttggc tgaagccggc ggcaagggac gtgccgacag
    20821 tcagggtcag aatcagaagg atggcgcggc gcataagttt ctccaaattg aaaacggctt
    20881 tattttatgg tttggcggga agggctgcaa gcaatcgggg tataatctga cctgattttc
    20941 attttaaagc ggtgtcgaac catgaacagc gaaactttag acgtaaccgg attgaaatgt
    21001 cccctgccga ttttgcgggc aaaaaaggct ttggcgcaaa tgcggcaggg cgaggtattg
    21061 accgttctgg cgaccgacgg cggcgcgccg ggggactttg aggctttttg ccgccaaacc
    21121 ggtcatgtgc tgttggattc ttccgaacag gacggcgtgt tcacactggt cgtcaagcac
    21181 aaataaatgc cgtctgaaat gcggatgtcc tgattgatgt tattgctgtc ggcatttgtg
    21241 cttgctgcgt gtaatgccca aaaccggcaa tccggtgtac ggcatcagga ttttgcacgc
    21301 gtagccgtcg atttgtttcg gagtgcctgc gtcctgactc aaggtagttt cgaaccggtt
    21361 tccaagtttg ccgctgtcgg acattttgtc cctgtcggaa aggaagacct gtcccgtctg
    21421 ccgcccgctg ttgcagaacc cgatgtacag gcgttgtgga cgcttgaacg cgatggcggc
    21481 atttattatt tgagcctgac gcgcgacagt tgcagtgtga aagccgagcg ggcagacagt
    21541 gccgcgcttt tggaacattt ctcagggctt gtccgacagc cgccgaaaaa tgc
//
LOCUS       DAXDSD010000026        21322 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXDSD010000026 DAXDSD010000000
VERSION     DAXDSD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21322
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.026"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(247..322)
                     /locus_tag="ACFB2Q_000768"
     tRNA            complement(247..322)
                     /locus_tag="ACFB2Q_000768"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:His,seq:gtg)
     gene            complement(350..426)
                     /locus_tag="ACFB2Q_000769"
     tRNA            complement(350..426)
                     /locus_tag="ACFB2Q_000769"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Arg,seq:tct)
     gene            complement(451..528)
                     /locus_tag="ACFB2Q_000770"
     tRNA            complement(451..528)
                     /locus_tag="ACFB2Q_000770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(491..493),aa:Pro,seq:tgg)
     gene            751..1605
                     /gene="folD"
                     /locus_tag="ACFB2Q_000771"
     CDS             751..1605
                     /gene="folD"
                     /locus_tag="ACFB2Q_000771"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC0917836.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAILERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            complement(1667..2248)
                     /locus_tag="ACFB2Q_000772"
     CDS             complement(1667..2248)
                     /locus_tag="ACFB2Q_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC0917837.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            2423..3538
                     /gene="asd"
                     /locus_tag="ACFB2Q_000773"
     CDS             2423..3538
                     /gene="asd"
                     /locus_tag="ACFB2Q_000773"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC0917838.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            complement(3631..4161)
                     /locus_tag="ACFB2Q_000774"
     CDS             complement(3631..4161)
                     /locus_tag="ACFB2Q_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC0917839.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(4171..4515)
                     /locus_tag="ACFB2Q_000775"
     CDS             complement(4171..4515)
                     /locus_tag="ACFB2Q_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917840.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            complement(4502..5281)
                     /locus_tag="ACFB2Q_000776"
     CDS             complement(4502..5281)
                     /locus_tag="ACFB2Q_000776"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC0917841.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            complement(5452..6873)
                     /gene="cysS"
                     /locus_tag="ACFB2Q_000777"
     CDS             complement(5452..6873)
                     /gene="cysS"
                     /locus_tag="ACFB2Q_000777"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC0917842.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            complement(6889..7941)
                     /locus_tag="ACFB2Q_000778"
     CDS             complement(6889..7941)
                     /locus_tag="ACFB2Q_000778"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HFC0917843.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGVESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFAEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            complement(7938..8885)
                     /locus_tag="ACFB2Q_000779"
     CDS             complement(7938..8885)
                     /locus_tag="ACFB2Q_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC0917844.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIVLETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            complement(8939..10093)
                     /gene="obgE"
                     /locus_tag="ACFB2Q_000780"
     CDS             complement(8939..10093)
                     /gene="obgE"
                     /locus_tag="ACFB2Q_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC0917845.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            10372..10902
                     /locus_tag="ACFB2Q_000781"
     CDS             10372..10902
                     /locus_tag="ACFB2Q_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC0917846.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            complement(10937..11812)
                     /gene="rsmI"
                     /locus_tag="ACFB2Q_000782"
     CDS             complement(10937..11812)
                     /gene="rsmI"
                     /locus_tag="ACFB2Q_000782"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC0917847.1"
                     /translation="MFQKHLQKAPDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGRLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            11859..12206
                     /locus_tag="ACFB2Q_000783"
     CDS             11859..12206
                     /locus_tag="ACFB2Q_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC0917848.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            12211..12804
                     /locus_tag="ACFB2Q_000784"
     CDS             12211..12804
                     /locus_tag="ACFB2Q_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC0917849.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            12868..13476
                     /locus_tag="ACFB2Q_000785"
     CDS             12868..13476
                     /locus_tag="ACFB2Q_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC0917850.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            13484..14134
                     /locus_tag="ACFB2Q_000786"
     CDS             13484..14134
                     /locus_tag="ACFB2Q_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917851.1"
                     /translation="MPFEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAAS
                     SEPAQPDGTEESGSGLPPPAAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVL
                     PTNAPHPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPAKPHKEILDNLF"
     gene            14174..14953
                     /gene="map"
                     /locus_tag="ACFB2Q_000787"
     CDS             14174..14953
                     /gene="map"
                     /locus_tag="ACFB2Q_000787"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC0917852.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            15052..15351
                     /locus_tag="ACFB2Q_000788"
     CDS             15052..15351
                     /locus_tag="ACFB2Q_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC0917853.1"
                     /translation="MKIFENIEDVKAIRKKTGMNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECLDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKAERKKQG"
     gene            15589..15960
                     /locus_tag="ACFB2Q_000789"
     CDS             15589..15960
                     /locus_tag="ACFB2Q_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC0917854.1"
                     /translation="MKLLTTAILSSAIALSSMAAAGTDNPTVAKKTVSYVCQQGKKVK
                     VTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSYR
                     KQPIMITAPDNQIVFKDCSPR"
     gene            complement(16147..17613)
                     /locus_tag="ACFB2Q_000790"
     CDS             complement(16147..17613)
                     /locus_tag="ACFB2Q_000790"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC0917855.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGADGVINPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCRYLQKRYDVFKTQKLFENMEFSTDRNKISDWAPLIMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDWQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGLPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSFMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(17997..18587)
                     /locus_tag="ACFB2Q_000791"
     CDS             complement(17997..18587)
                     /locus_tag="ACFB2Q_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC0917856.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLACCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            complement(18648..19229)
                     /locus_tag="ACFB2Q_000792"
     CDS             complement(18648..19229)
                     /locus_tag="ACFB2Q_000792"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC0917857.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKRGR
                     KIGVYWPMGKELRLGGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            19307..19540
                     /locus_tag="ACFB2Q_000793"
     CDS             19307..19540
                     /locus_tag="ACFB2Q_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917858.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMGQQEQAESEQQRAQRQKDSGTKP"
     gene            19713..20441
                     /gene="rpsB"
                     /locus_tag="ACFB2Q_000794"
     CDS             19713..20441
                     /gene="rpsB"
                     /locus_tag="ACFB2Q_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC0917859.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            20570..>21322
                     /gene="tsf"
                     /locus_tag="ACFB2Q_000795"
     CDS             20570..>21322
                     /gene="tsf"
                     /locus_tag="ACFB2Q_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC0917860.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKEN"
BASE COUNT         5228 a         6032 c         5300 g         4762 t
ORIGIN      
        1 gattcaaaat cagggacaga ccaagcgtca accatcaaag cctccgacaa aaaccataaa
       61 agacagaaaa acccacatga tacagaagca tatgcgaaag gcaaagccgg cggcgcaaat
      121 gccgtatgaa gcggtttccg tatgaccggc ccggctttca gacggcatac cgccgcaccg
      181 gtacgacggc gcggacagta cgcgcaaaca ggaaaagacc cgtaccgaaa agtacgggcc
      241 tttatctggg gtggctgatg gggctcgaac ccacgacaac cggaatcaca atccggggct
      301 ctaccaactg agctacagcc accataaaaa cggttttcaa tcaaattctt ggcacgcccg
      361 acaggaatcg aacctgtaac ccccgactta gaaggtcggt gctctatccg gttgagctac
      421 gggcgctcat gccgattcgt gctgattgat tggtcggggc ggtgggattc gaactcacga
      481 ccctctgctc ccaaagcaga tgcgctaacc gggctgcgct acgccccgac ttgaagaagc
      541 ggaatataca gttcagggaa agatgcgtca acatttattt tcaaaacacc aagatgaaaa
      601 atatagtttt ttgatttgaa aaaatattta atccgtccaa acagccgtat tttatttcgg
      661 ggcaaattta ttttcggcat tctgctgtaa aaacaaacgg aaaatgcgat aattttcagc
      721 attttctacc tgtttaacaa aaggacggat atgtcggcac aactgatcaa cggtaaagaa
      781 gtttcgcaaa aacacctgca ggcgattgcc gaagcggtgg cgcaacgcca acaggacaat
      841 ctgcacacgc cttgcctggc cgtggttttg gtcggaggcg accctgccgg cgcagtttat
      901 gtccgcaaca agaaaaccgc ctgccaaaaa tgcggcatca agtcgctgtc gtacgaactg
      961 cccgaatcaa catcgcagga agaactgctg gcattggtcg accgtctgaa tgccgattcc
     1021 gaagtggacg gtattctggt tcagcttcca ctgccgaagc acctcgacag ccaagcgatc
     1081 ttggaacgta tttcgccgga taaggacgtg gacggcttcc atccttacaa tgtcggcagg
     1141 ctggcggtca aaatgccgct gatgcgcccg tgtacgccca agggcgtgat gacgcttttg
     1201 gaggcttacg gcattgatcc gaaggggaaa aaagcggtcg tggtcggcgc gtcgaatatt
     1261 gtcggccgcc cgcaggcgtt ggaattgctg ctggcgcgcg caacggtaac ggtctgccac
     1321 agtgcgaccg aaaatcttgc cgatgaagtt gccgccgccg atattttggt ggtcggcgtg
     1381 ggcattccga actttgtcaa aggcgggtgg atcaaacccg gcgcggtcgt tatcgatgtg
     1441 ggcatcaacc gtttggacga cggcagcctg tgcggcgacg tggaatttga aacggcaaaa
     1501 gaacgggcgg cgatgattac gcccgttccc ggcggcgtgg gtccgatgac gattgccaca
     1561 ttaatggaaa acactttgca cgcagcttcg ctgcacgatg cttgagcggt tctgaagata
     1621 aaaatgccgt ctgaaagcct ttcagacggc attttgccgc gtccgtttat ttgggcagct
     1681 tgacgacaac cgtatccgcc aatatgtcgt aaagcgtgcg gcggtcgcgt ttgaccataa
     1741 agagcaggac aaagttggca aggaatgcca gcaggttgat ggcgttttcc ccgttttcac
     1801 ctactgcaag accgataacg gcggcaataa tggcaaccaa aaccgaccac gcgatttcgc
     1861 gtaccaaaac cgtgccgaca aaacccggat tgcggccgtc ggttttcaac acgcggattc
     1921 tcatgatttt cttacccaat gactgcccgt ccctgctcat atagtagatt tggatgacgg
     1981 tgtacgccaa aatgcccgcc agtccgaccc aaaaggaagt catgcccaaa agcagcccga
     2041 atatttcctc acgaccgcca atcctgcctt catttttgac ggcgaaggca atcagtccga
     2101 caaacggcac caacaaaatc aaaaaggtaa acaattggtt cagcagcgcg gcaagtatcc
     2161 ggtcgcctgc gccggcaatt ccgacttcaa tttcctgccc gttgcggttg tcggataccg
     2221 cgtcggtgta gtcgtttttt tcttccatat ccgttcctga taattgttct taaacagatg
     2281 gaattctacc gccctactgc tgcaaacgcc aatcccaaaa agaaaattcg ataaagaact
     2341 ttacattttc ccaatacggc gttaaaacgc tttctttacg ccatacataa ttttattaac
     2401 gattttcctc aaggagcaat acatgaaagt aggtttcgtc ggctggcgcg gtatggtcgg
     2461 ttcggttttg atgcagcgta tgaaagaaga aaacgacttc gcccacattc ccgaagcgtt
     2521 tttctttacc tcttccaacg tcggcggcgc ggcacctgat ttcggtcagg cggctaaaac
     2581 attattggac gcgaacgacg ttgccgagct ggcgaaaatg gacattatcg tcacctgcca
     2641 aggcggcgat tacaccaaat ccgtcttcca agccctgcgc gacagcggct ggaacggcta
     2701 ctggattgac gcggcgtcct cactgcgcat gaaagacgac gcgattatcg ccctcgaccc
     2761 cgtcaaccgc aacgtcatcg acaacggtct caaaaacggc gtgaaaaact acatcggcgg
     2821 caactgtacc gtctccctga tgctgatggc tttgggcggc ctgttccaaa acgatttggt
     2881 cgaatgggcg accagcatga cctaccaagc cgcttccggc gcgggcgcga aaaatatgcg
     2941 cgaactcatc agcggcatgg gcgcgattca cgcccaagtg gcggacgagc ttgccgatcc
     3001 ttccagcgcg attctcgata tcgaccgcaa agtgtccgat ttcctccgca gcgaagacta
     3061 tccgaaagcc aacttcggcg taccgctcgc cggcagcctg attccgtgga ttgacgtgga
     3121 tttgggcaac ggccagtcca aagaagaatg gaagggcggc gtggaaacca acaaaatcct
     3181 cggacgcagc ggcaatccga ccgtcatcga cggcctgtgc gtccgcatcg gcgcgatgcg
     3241 ctgccacagc caagccatca ctctgaagtt gaaaaaagac ctgcctgttt ccgaaatcga
     3301 agcgattttg gcaggcgcga acgactgggt gaaagtcgtc cccaacgaaa aagaagccag
     3361 catccgcgag ctgacgcccg ccaaagttac cggcacgctg tccgtccctg tcggacgcat
     3421 ccgcaaactg ggcatgggcg gcgaatacat cagcgcgttc accgtcggcg accaactttt
     3481 gtggggtgca gccgaaccga tgcgccgcgt gttgcgtatc gtgttgggca gcctgtaagc
     3541 cctgtttgaa tggaaatgcc gtctgaagcc tgtttcagac ggtattttcc ttgcaacccc
     3601 ggttaaccga aattccgata aagcgaccgt tcacgccagc aacatttcct gcatcagctt
     3661 cataccccac tgccagccgc cgagcatacc gttcaaactg cccgaatgcg gggaaaccaa
     3721 caggcgggcg ttccacaaat ccgcctgttt ttgcgcccaa ccgtgcggca cgccgccgtg
     3781 ttcggacacc accaatgcgg cacggcaagg acagcggacg cgttggaaag tgtgttccgc
     3841 atcgtcggga aaaatatcgg gacgcggaga tacgagaatg ataccggcga gtttcttctg
     3901 cgtcaggatg tcggcgcggt acagccacgc caagaatgcg gccgcgcccg cgccgtgtgc
     3961 gacaacggcg atatgcctgc cctgtatccg ttcgaatgcc gtctgaagcg cgctttgcca
     4021 cgcttccgtg ctgccgtttg cggacgcttc ggacatctgc acaacgggat aactgaccgc
     4081 ccaacggtct atccacattt ccgcttcatc cgcatcgcgt atcagccaca gcgtcaaatc
     4141 ctcaagctcg aaactttcca ttgtccgcga ctatttgagc aggtcccgga gggtaaaggc
     4201 gatgagcagc gaagcgggta cgctcaatat ggcgcagacg gtcaggcaga caaaaatatt
     4261 caccacccgc cgccagcctt tccaacccaa acatttggcc gcaatcagca ggcagggcag
     4321 acaaatcagc agccacacca acgcccatat cgggtttgcc ttggtcggcg caagccagcc
     4381 ttgcatccgc gacaacataa acaccgccca caccaatatg ggcaggataa acgcagcgac
     4441 aacccatgcc gcgcctattc ctgtttttcc gtccacattc caatcatatt tacccaaaac
     4501 cttattcggc agcatagtca tactccacaa ctaaaggggc gtgatccgag aatttttcat
     4561 ctttataaac gtgtgcggac acggctttgg cggcaagttc gggcgtaacc atctgataat
     4621 cgatgcgcca cccgacatct ttcgcatacg cctgcccgcg gttgctccac catgtataac
     4681 ccggcacatc gggatagagc gtgcgccaca tgtccgtcca gcccagcgta tggatgactt
     4741 tgcctatcca ctcgcgctct tcgggcagga agcccgaatt tttctgattg cctttccaat
     4801 ttttcaggtc gatgttttgg tgggcgatgt tccagtcgcc gcagacgacg atgtcgcgcc
     4861 cttcgttttt catcgcttcg agcatagggt aaaacgcatc gaggaaacgg tatttcacct
     4921 gctggcgttc ttccgcgctg ctgccgctgg gcaaataaag cgaaatgacg cccaacctgc
     4981 cgaaatcgca acgcacaaac cgcccttcgc ggtcgaattc ctcaatgccc atgccgattt
     5041 gcacattgtc gggtttgcgt ttgctgtaca ccgccacacc gctgtaaccg cgcttctcgg
     5101 cgcaatgcca atagccgtgc atcccgtgcg gatttttcat atcggcagac aaatcagcct
     5161 cctgcgcttt gagttcctgc acgcagacaa tgtctgcgcc cgatgcggcg atgtattcgt
     5221 aaaaaccttt tttgtaggcg gagcggatgc cgttgacgtt ggcggaaatg attttaagca
     5281 taataaaaat aagttctcac aatgttccca tacacaaagc ggcacccggt gcgccgcccg
     5341 gtatttccca ttcggtcctg cctgctgaaa acggcggggc ttcaagcggg aaatgccgtc
     5401 tgaaaacgaa aaaagggcaa aaggcggtac ttttgccctt tccgatacag ttcaaccgcg
     5461 ccgccaagtc gtgccgccgg cgctgtcttc caaaatgatt ttgtgttcgt tcagaaggtc
     5521 gcggatgcgg tcggactccg cccagttttt atccgcgcga gcctgtttgc gtcgggcaat
     5581 caagtcttcg atttcttcgt tggagaggcc gtctgaaact gcgccgcctt ggaggaactc
     5641 aatcgggttg cgttgcagca ggccgatgat gccgcccaaa gctttcaggc agccggcgag
     5701 gtgtgcgtca ttggttttgt tcacttcgcc tgccagctca aacaacacgg caaccgcttc
     5761 gaccgtaccg aaatcgtcgt tcatggcggc gtagaaacgg cgggtgtagc cgttggcgtt
     5821 ttcagacaaa tcaaactcag ccgccggtgt gtttttcaag gtggtgtaca gacgggtcaa
     5881 cgcgcctttc gcgtcgtcca aatgcgcgtc ggagtagttc aacgggctgc ggtagtgggc
     5941 gcgcaggatg aagaaacgca cgacttcggg gtcgtattgt ttcaatactt cgcggatagt
     6001 aaagaagttg cccagcgatt tggacatttt ttcgccgtcc acacggatga agccgttgtg
     6061 cagccagtat ttgacgtggc tggcaatact ttgaccgtgg tgggtttgcg cgtggtcatg
     6121 accgcaggtg tgaccgctgg caccgacgct ttgggcgatt tcattttcat ggtgcgggaa
     6181 ctgcaaatcc gcgccgccgc cgtgaatgtc gaacgtatcg ccgaacaggt tttcactcat
     6241 cgcggaacat tcgatgtgcc aacccggacg gccgttgccc cacgggcttt cccacgccgg
     6301 ttcgcctgct ttcgccgctt tccacaatac aaaatcaagc ggatcgcgtt tgaaaccgtc
     6361 cacttccacg cgttcgcccg cacgcaggtc gtccaacgat ttgcccgata attgtccgta
     6421 agcggcaaac tcgcgcacgg cgtagtaaac gtcgccgttt gcggcaggat atgccttgcc
     6481 gttttgaatc agggtctcaa tcatggcaat catttgcggg atgttttccg tcgccttcgg
     6541 ctcgatgtcg ggacgcaaca cgcccaaagc atcggcatct tcgtgcatcg cctgaatgaa
     6601 acgcgcgttc agttcgccga tggtttcgcc gttttcagcc gcacgggcga tgattttgtc
     6661 gtcgatgtcg gtgatgttgc gcacataagt gagcggataa ccgcactcgc gcaaccagcg
     6721 ggcaatcatg tcgaacacca ccatcacacg ggcatggcct aagtggcaat agtcgtaaac
     6781 ggtcatgccg cagacgtaca tacgcacgtt ttcagggtcg atgggggcga agggttcttt
     6841 ttgacgggta agggtgttgt agatggcggt catattcatc aagtaagttt agaaatgagt
     6901 aacagtaatg tcatatccgc gattttgggc gaaatctaaa aattcattgg cttcattaac
     6961 ggcaggaaat tcacgcataa tttcagtttt aatattttcc aacaacgctt ctgcaatcgg
     7021 atattggcct ctcaatgctt tttcgtacca acttatcaaa tcatcctcag ttacgatgtt
     7081 ttgaattcgg cttttccagc ctatttgcgt cagtaatgaa acaatcaccg attgttctgc
     7141 ctttttgcag acaatgacaa ttctatctgc gctaatggag gatacaatat cttctgccaa
     7201 ctcttcgtct aaagagagat gtttgacctg tatggccaat ccgaagttag accacatatc
     7261 caaccctcta tcggcagcat tggttacgcc tacacgatgg atttttgcag gaagttttaa
     7321 atgctcattt ttcggcatag tgatgatttt ttcggcaaaa tcttgatatt cctcccataa
     7381 aaacaggttt tccttaggaa aatcaattga aaccgttatg cctaattctg aaaccaatgc
     7441 atcaaacaac gcataaacaa cgatttcata tatcttgtct atacttcttt tcaatcccgg
     7501 ctcgagccaa aataaattta gaaattcaga cagttggaag gagtatctgt ctgtattgcc
     7561 gacataagcc aatgcttcgc tcatttggga aaaacgattg aaaaactgtt tataaatgta
     7621 cgattctacc ccgccgtccg actgtctgtt taatgttccc aataccgcta gtttttcagg
     7681 cggtattgca tttttttcaa acagattatc ttggaatttt gcagatgaag tagaaatccg
     7741 ccctaaaaac cgacggcaga tttcatccct ccagcgttta gattggttgc ggtaagtatc
     7801 taaatttaaa aaatccaact gttttataca acggtcatga tataaaattt ctgcaatttg
     7861 aatgggttta taaaggtgga cacgggattt tttgataatg ccgtccaatg cttctttggc
     7921 ttgttgttct tcaagcgtca tcctgcctcc aataaatcca attgcccttt ttgttcgacc
     7981 ttgccggaaa gagaagcgag catattttcc gcgatggctt ctattacagg gacggataca
     8041 gaatttccgg cgactttcct gacttgcgag taggggatat tgatttcaaa atcatcggga
     8101 aaaccttgta atcttaacat ttcccttcct gttagccttc ttactccgtt taccaccaaa
     8161 taattgtaac ttcccccggc ccttaaggca caggaatatg gaagtgcgga aacattcccg
     8221 ccaatatttt catgccaaat tgatggggtg ggcggagctt tttttaaggc tgccaaacgt
     8281 ttttgtttca aggtatctga taaaaaatag cttggttcga catctcgatc gttttccagc
     8341 agttcgccca atggccgata ttggtttatg ggttcgggaa agtaaaaagg gatgttgtct
     8401 gaaaaaccca caatataaat tctctcgcgt ttttgcggca atccgaaatc caaagtattt
     8461 aaaaccttaa aataaaccgt ataaccaagc tgtttaaggg tttctaaaac aatacggaaa
     8521 gtcctgcccg aatcgtgcgt agtcagccgc ttaacgtttt ccagaagaaa agccttcggc
     8581 tgtttggttt tcaggatttc ggcaatattg aaaaacaatg ttccgcgcgt atcttcaaat
     8641 cccaatccct tacctgcgat actgaaaggt tggcacggaa atcccgcaag cagaatatca
     8701 tgatcgggaa tatctgaagg atcgatgccg ttaatgtcgc caaaaggttt ttctccaaag
     8761 tttgcttcat acacttgtcg ggcatatttg tcccattccg atgagaatac attggtgcac
     8821 ccgtattttt caaagcctag gcgtattccg ccaatgcctg aaaataaatc tattgttttg
     8881 tacatagcac atctctgcag aatgagccgc ctgaaaactt tcagacggcc ttgtcctttt
     8941 actccggctt aaacacgcca gtatccgttt taggctgctg ttcgataatt tcaacatttg
     9001 ccgctgcttt ctccgcttcg gctttttcag cctcgatacg ttttttctcg gccaggtatt
     9061 ggttgatttg gtgtaccaac tcctgcgtgc cttgatgtgt cagcgcgctg atttggaaga
     9121 ggcgcggggt ttccatgtcg aattggaaac ggtcatcagg ttcggggtag tcccagccga
     9181 cggcttcgag gaaggcggct gttcgcgccc gggcttcttc ttcgtcgagc atatcgagtt
     9241 tgttcagcac cagccagcgc ggtttgccgt aaagttcttc gtcgtatttg cgtaattcgt
     9301 tgatgatggc gagtgcttct tcggcgggat tgacggtttc gtcgaagggt gccaaatcga
     9361 caacgtgtaa cagcagaccg gtgcgtgata ggtgtttgag gaagcggtgg ccgaggcctg
     9421 cgccttctgc cgcgccttca atcaggccgg ggatgtcggc catcacgaag ctgtggtttt
     9481 cgtcgatgcg taccacgccc aagttcggat gcagggtggt gaaggggtag ttggcgattt
     9541 tggggcgtgc ggcggagacg gcggtaatca gggtggattt gccggcgttg ggcatgccta
     9601 acaagccgac atcggcgagg actttgagtt cgagttgcag ggaacgggtt tcgccttctt
     9661 cgccgggcgt ggattgtttt ggggcgcggt tgacggagga tttgaagtgg atgttgccca
     9721 agccgccttt gccgcctttg gcgaggcaga cgcgctgtcc gtggtaggtg aggtcggcaa
     9781 cgatttcgtc ggtgtcgagg tcgcggataa gggtgccgac gggcattttg aggacaatgt
     9841 cgtccgcacc tgcgccgtag cggtcggaac cgtggccttt ttcgccgttt ttggcttggt
     9901 agcgtttgac gaagcggtat tcgacgaggg tgttggtgtt ttcgtcggct tctgcccaga
     9961 cgctgccgcc tttgccgccg tcgccaccgt ccgggccgcc gcggggtacg aatttttcgc
    10021 ggcggaaact ggttgcgcca ttaccgcctt tgcctgcggc gacttcgatt tttgcttcgt
    10081 cgatgaattt cattaaatgc tcttgtttgt tggttttaaa tagggtttca ggcggattgc
    10141 catatgtttt gatgccgtct gaacagattt tctgccgaca ttataaggta taagcggtat
    10201 ttcaatatac cgcggagaag ctatttgttc tgcccatctt aatgaatttt taagcaaatc
    10261 ttcagcctgc aaacaaaata tgtccaactt ctttggtaca atcgcgcctt tttgacattc
    10321 cgacccgacg gaatgtccgt tcaaaccgtt acatatgata aggatttttt tatgaacaca
    10381 aaccaacctg ccgtttacga cccgttgaca cgcgcgctgc actggctgac cgttgccggc
    10441 ttcatcggca ttctgaccac cattgtcctg tggacaattt acgaagaggc ggaatgggcg
    10501 ggcagcctgt tcggcctgca caaatctttc ggtttcctca tgctgacggt gattgcattg
    10561 cgcatcgtgt gggcggttgc caaccgcgcc aagcgtccgc aaagcgacag caaggcggca
    10621 gcggcaggac acggcattct gtatctgctc atgcttgccg ttcccgttat cggcatgatc
    10681 cgccaatacg gtggcggacg cggcccgttg aaagtgttcg gcgttgaagt aatgcagggt
    10741 tcgccggaaa aaatcgagtg gatggcaaac ttgggcaaca cgttccacgg caatttgggc
    10801 tggctgctgt ttgccgccgt cgtcggacac gtcgccatgg tcgtcgtcca tcgtgttcaa
    10861 ggcaaagacg tgctgtaccg catgacgggg cgtgtccgtt gattccgttc gcactatggt
    10921 gccggtccat ccggcatcat ttgtttttcc acgacagtgc caaatcgtac aaagcctttt
    10981 tgccctcacc tgtaatcttg gcggcaagct ccgccgcctg cttggtcggc agctcggccg
    11041 caaggatttt catcgcattt tgcgcagact cggacaagcc ttcgtgtttt tcatcctgcg
    11101 ccggataaag caccaacacc atctcgccgc gcgattggtt gccgtccgcc gccaatgccg
    11161 tctgaatttc cccaaccgtg ccgcttaaga acgtttcaaa cgttttcgtg atttcgcgcg
    11221 ccagcatcag acggcgttcg gggaacaatt ccgccatatc ggcaagcgtt gccccgattc
    11281 ggtgcggcgt ttcaaacatg acgacaggaa atgccgcccg cacccatttg gcaaacaatt
    11341 tcctacgttc gcccgatttc ggcggtacaa aaccgttgaa ataaaaatcg gattccgcca
    11401 caccggccac actcaacgcc gccattaccg cgcttgcgcc cacgacggga acgactttga
    11461 accctgcttc gcgcacgcgg cgggcgagtt tcgcgcccgg gtcgcacacg gccggcgtac
    11521 ccgcatcgga aacctgcgcc acaaccaggc cgtctgaaag gaaaccgatt accttgtccg
    11581 ccatctgccg ctcgttgtgt tcgcgcacac tgaccaacct gccctgaatg ccgtacgcgc
    11641 tcaaaagctg cgcagtaacg cgcgtgtctt cggcacaaat gatgtccgcc ttttgcaata
    11701 ccgccaaagc gcgcagggta atgtctgcca aattgccgat gggcgtggca accacgtata
    11761 atgtccctcc gacgacgctg tcgggggctt tctgcaagtg tttctgaaac ataaaaaatg
    11821 ccgtctgaaa aacagacatt ataaaggtta aaccgattat gcgcttaaac cacaaacagg
    11881 gtacggcagg ggaagatgcc gcacttgcct tcctccaatc ccaaggctgc acgctgcttg
    11941 cccgcaactg gcactgcgcc tacggcgaaa tcgacctgat tgtcaaaaac ggcggcatga
    12001 ttctgtttgt tgaagtaaaa taccgcaaaa accaacggtt cggcggcgcg gcatacagca
    12061 tttctccgtc caaattattg aaactgcaac gaagtgtaga gtattatctg caacagaaca
    12121 ggctgacaaa cgtaccgtgc cgcctcgatg cggtacttat cgaaggaaac cgcccgcccg
    12181 agtggataaa gaatattaca ggttgacgat atgacgacat tacaagaacg cgttgccgca
    12241 cattttgccg aaagcatccg tgccaaacag gaagccgaaa aaatactggt cgagccgacc
    12301 gtacaggctg ccgagctgat gctgcaatgc ctgatgaacg acggcaaaat cctggcctgc
    12361 ggcaacggcg gttcggctgc cgacgcgcaa cacttcgccg ccgaaatgac cgggcgtttt
    12421 gagaaagaac gcatggaact cgccgccgtc gccctgacca cagacacctc cgcgctgacc
    12481 gccatcggca acgactacgg tttcgaccac gtattcagca aacaagtgcg cgcgctcgga
    12541 cgtgcaggcg acgtcctcgt cggcatttcc acctccggca attccgccaa cgtcatcgaa
    12601 gccgtcaaag ccgcgcacga acgcgatatg cacgtcatcg ccctgaccgg ccgcgacggc
    12661 ggcaaaatcg ccgccatgct caaagacacc gacgttctgc tcaacgtccc ccatccgcgc
    12721 accgcccgca ttcaggaaaa ccacatcctg ctgatacacg ccatgtgcga ctgtatcgac
    12781 tccgtactgc tggaaggaat gtaacccttt tcagacggca tggcgcaaag caatgccgtc
    12841 tgaaacgccc aagaaaggaa gtccccgatg aaacccaaac cacacaccgt ccgcaccctg
    12901 attgccgccg tcctcagcct tgccctcggc ggctgcttca gcgcagtcgt cggcggggcc
    12961 gccgtcggcg cgaaatccgt catcgaccgc cgaaccaccg gcgcgcaaac cgatgacaac
    13021 gttatggcgt tgcgtatcga aaccaccgcc cgttcctacc tgcgccaaaa caaccaaacc
    13081 aaaggctaca cgccccaaat ctccgtcgtc ggctacaacc gccacctgct gctgctcgga
    13141 caagtcgcca ccgaaggcga aaaacagttc gtcggtcaga ttgcacgttc cgaacaggcc
    13201 gccgaaggcg tatacaacta cattaccgtc gcctccctgc cgcgcactgc gggcgacatc
    13261 gccggcgaca cttggaacac gtccaaagtc cgcgccacgc tgctgggcat cagccccgct
    13321 acacaggcgc gcgtcaaaat cattacctac ggcaatgtaa cctacgttat gggcatcctc
    13381 acccccgaag aacaggcgca gattacccaa aaagtcagca ccaccgtcgg cgtacaaaaa
    13441 gtcattaccc tctaccaaaa ctacgtccaa cgctgactcg gcaatgccgt ttgaaccgcc
    13501 ttcagacggc attgcccgac accccaaaag cacaatcaaa atggcaaaaa aaccgaacaa
    13561 acccttcagg ctgaccccca aactcctgat acgcgccgta ttgctcatct gtatcaccgc
    13621 catcggcgca ttggcagtag gcatcgtcag cacattcaac ccgaacggcg acaaaaccct
    13681 ccaaaccgaa ccgcaacaca ccgacagccc ccgcgaaacc gaattctggc tgccaaacgg
    13741 cgccgtcgga caagatgccg cccaacccga acaccaccac gccgcctcat ccgaacccgc
    13801 acagccggac ggcacagaag aaagcggcag cggactgccg ccccctgccg cacccaagaa
    13861 aaaccgggtc aaaccgcgcc cttcggatgc ggcccgggca gccgattcgt taaccggcac
    13921 cggaacacaa gctgaaaaca cactcaaaga aacccccgta ctgcccacaa acgcccccca
    13981 tcccgaaccc cgaaaagaaa cacccgaaaa acaggcgcag cccaaagaaa cacccaaaga
    14041 aaaagaaacg cccaaagaaa accataccaa accggacacc ccgaaaaaca cgccggccaa
    14101 accccataaa gagattctcg acaacctctt ttgacccggc acggcaggca cacccgcaat
    14161 ccaaggaagc attatgaacg gcatcatcat caaaaccccc gaagaaatcg aaaaaatgcg
    14221 cgagctgggc aaactcgtcg ccgaagccct cgactacatc ggacaattcg tcaaacccgg
    14281 catcaccacc gacgaaatcg acaaacttgt ttacgactac cacgtcaacg tccaaggcgg
    14341 ctatcccgcc cccctccact acggcaaccc gccctacccc aaatcctgct gcacctccgt
    14401 caaccacgtc atctgccacg gcattcccga cgacaagccg ctcaaagaag gtgacattat
    14461 caacatcgac ctcaccatca aaaaagacgg tttccacggc gactccagcc gtatgtttac
    14521 cgtcggcaaa gtctccccca tcgcccaacg cctgatcgac gtaacccacg cctctatgat
    14581 ggcgggcata gaagccgtca aacctggcgc gacgctgggc ggcgtaggtt acgcctgcca
    14641 gcaggttgcc gaaaatgcag gttattcggt tgtacaagaa ttctgcggac acggcatcgg
    14701 gcgcggtttc cacgaagccc cgcaagtgtt gcactacgga aaaaaagggc agggtcccgt
    14761 tctaaaaccg ggtatgattt ttaccgtcga accgatgatc aaccaaggca aacgccacct
    14821 gcgtatcctc aacgacggct ggacggtggt taccaaagac cgctccctct ccgcccaatg
    14881 ggaacacgaa gtcttggtga ccgaaaccgg ctacgaaatc ctcaccgtca gccccgccac
    14941 cggcaaaccc tgaagctgta tgcaatataa aaacaggctt gacccggcac attacgaaaa
    15001 caaagcaaat cggaatttgc cccgcaacca gaaaaactta aaggaagttt tatgaaaata
    15061 tttgaaaata tagaagatgt taaagccatc cgtaaaaaga ccgggatgaa ccagatagac
    15121 ttctggggca aggtcggcgt tactcaatcc ggaggttcac gctacgaaac cggccgcaag
    15181 atgcccaaac ccgtacgcga actgctccgc ctcgtccata tcgaatgcct cgatttggca
    15241 aaagtcaaca aaaaagatat ggaaatcgcc gccctgttga aaaaacacca tcccgacctg
    15301 tatgccgaat tgtccaaaca gaccaaggcc gaaagaaaaa aacaaggtta aaccgcaacc
    15361 tccggatgcc cggaggtttt tcatttccga aaaatgcaaa caatgccgtc tgaaacaccg
    15421 gacaggccgc cgtatcccgc ctgccgcccc tgcctcaaac cgccgaaccg cccgaacctg
    15481 cctttttaca aactttatcc aatttcctgt ttatttcagg atacgccgac attagactgt
    15541 caaacagtcc gaaacgggca aactccacat ctatccaaag gaataaaaat gaaacttctg
    15601 accactgcaa tcctgtcttc cgcaatcgcg ctcagcagta tggccgccgc cggcacggac
    15661 aaccccaccg ttgccaaaaa aaccgtcagc tacgtctgcc agcaaggtaa aaaagtcaaa
    15721 gtaacctacg gcttcaacaa acagggtctg accacatacg cctccgccgt catcaacggc
    15781 aaacgtgtgc aaatgcccat caatttggat aaatccgaca atatggacac gttctacggc
    15841 aaagaaggcg gttatgtgct gagcaccggc gcaatggaca gcaaatccta ccgcaaacag
    15901 cctattatga ttaccgcacc tgacaaccaa atcgtcttca aagactgttc cccacgttaa
    15961 tcaggcaaca aaaaacagcg ttttcatttc tgaaaacgct gttttttgac cgttccatta
    16021 ttcgcaaaag ggaaaaaacg attacctgcc ccgtgtatca aaacctgccc tgccggatga
    16081 agggcataac cggcagggac ggcgtcaaca ccatatgggg gtacggcttt tcttgaaaga
    16141 ttcggtttaa atatccaaca ctttcgcggt ataggcgata atttcatccg ccctttcagg
    16201 gttttcgttc aacttgatgc cgtaacccgg taccagctct ttcagacgac cttcccaaga
    16261 cggggcgcgc tcggggaagc attggtgcat cagccggatc atcagcggca cagcggtcga
    16321 tgcgcccggc gacgctccca gcaatgcggc gagcgagccg tcggcgtggg cgacaatctc
    16381 cgtaccaaac tgcagcacgc cgcctttttc ggagtctttt ttaatgattt ggacgcgttg
    16441 ccctgcggtg atgagttccc agtcgtcggg gtttgcctcg gggtagtatt ccagcaggga
    16501 ggcaaagcgt tcttctttgg ttttacgcaa ttcgcccagc aggtatttgg tcagcggcat
    16561 attcgcccag ccggcgcgca gcataggata gaggttgtcc atatggatgg acagcggcaa
    16621 atccataaac gagccttgct tgaggaagtt ggaacggaaa cctgcgtaag gaccgaacat
    16681 aaggtgtcgt ttgccgtcta cgttgcgtgt gtcgaggtgc gggacggaca tcggcggcgc
    16741 gccgacggaa gcctgcccgt acactttggc gttgtgttgt tcggcggttt cggggttgct
    16801 gttgcggaag aacaggccgg acacgggtaa gccgccgtag cctttgcctt cggggatgcc
    16861 ggatttttgc agcagggtca gtgcgccgcc gcccgcgccg aggaagagga agcgggtgcg
    16921 gagggtgagc tgccagtctg ggttgcgggt atcggcggtt ttgagcaccc acgcgccgtc
    16981 ggattcgcgt ttgatgtctt cgacgtggcg gttgaactcg gtttttacgc ccttgccctg
    17041 caaatatttc accatctggc gcgtcagccg tccgaaatcg acatccgtgc cttcggcgga
    17101 atagttggcg gcgacgggtt ggttttcgtc ccggccgcgc ataatcagcg gagcccaatc
    17161 ggaaattttg ttccgatcgg tggaaaattc catattttca aaaagtttct gcgttttaaa
    17221 cacatcatag cgtttttgca ggtaacggca gtggtcttcg ttcatcacca aagacatatg
    17281 cggcacggca ttgatgaagg aattgtcttc caacttgcct tccgcgacca gcgtcgccca
    17341 aaactggcgg ctgacatgaa actgttcggc aatattgagg gcgcgcgccg gattgatgac
    17401 gccgtccgca cccagcggcg catagttcaa ttcgcacagc gcggaatgcc ccgtgccggc
    17461 gttgttccac gcgtttgacg attccaacgc cacatcttcc aagcgttcaa tcagggtgat
    17521 ttcccaagac ggttcgagtt ctttgagcaa aacgcccaaa gtcgcgctca taatgccgcc
    17581 gcccaccaag acaacgtctg tcgcttcagc catggtttac tcctaaaaaa caggcatctt
    17641 ctgcccttat ggttatttgc cgtactacaa acgcctgaat cgcaaaagca ggggaaaccg
    17701 gcaatggtgt gtgtccgagc acgctgtttc ggggttggaa tgcgttgcaa gtatggcttc
    17761 cgacaccgct tcaggggctt gtaatatgtt atcgtgaatg tagtggattt tactgggaaa
    17821 tgcaaagttt ttctgtcgcc cgccaagccg gggaactgcg aaacaaaaaa taaaaatagt
    17881 tatttatcta tatatatcaa atttttaata gacaaaaaat caaaattgtt tatatattaa
    17941 tttttaaaag attgtcagca tatcgcgtta agttttttaa atttgagaat actgcctcac
    18001 acctgcacgc cataccctgc caacctgccg gtcaggattt ccctgttttt gcaccaatct
    18061 tccctcagca tactgtacac aaccgtatcg cgcacactgc cgtctttacg gagcatatgc
    18121 atacgcagca cgccgtcttt ttccgcgccc agccgttcga tggcacgttg cgaggcaagg
    18181 ttcagaatat ccgtgcgcca tcccgcgcaa cagcaagcta aaacatcaaa tgcgtaatcc
    18241 aacagcatga ttttgcaaca ggtgtttatc cgtttccgcc gtgccgatgc cgcataccat
    18301 gtgaatccga tatccaaacg cggaatctgc ggttcaaaat gataatacgc cgttgtcccg
    18361 accaccctgt ccgcctcttc atcgacaacc gcaaacgcca aacgcgttgc caatgctgtc
    18421 ccgatatagt ctgccaccct atccggatgg ggcgcggacg ttacccccag cttccaaacc
    18481 tccccatcgc aaaccgcctc gcgcaaaccc gtttcatgat gcacatccaa cggttcgagc
    18541 cgtacgccgc ccaatgacaa aaccggcagt attatccttt ccgacatcct tttctcccaa
    18601 tattccgcct tcagacggca tttccgcccg aaatgccgtc tgaacggcta aaaacacaat
    18661 atccccgctt ccgatacaaa accgtccagc ggcaggtcgt gcgcctcgcg tgggagcctg
    18721 tccaccaact ggcaggcaaa gcccacgccc acggttttcg cctgcaaacg gtatttcatc
    18781 gccgaaagcg tcgcatcgta atagccgcct gcctgcccca aacggtagcc ttcgcggtct
    18841 atgccgacaa gcgggacgag caataccgac aaaccgtgca cgcggatttt gcgccctgca
    18901 aactgaggga catgcagctt cgccctaccg cgcttgcgtt cccgttccat tccgcgttca
    18961 ggatacggcg taaaccacat ccgccgcgtg tgcggttcga tataaggcag atagagtttt
    19021 gcgccgcgtt tttgcgccgc gcggacaaag ccgcccaaac gcaattcctt gcccatcggc
    19081 caatacacgc cgattttccg accgcgcttg atataacgtt tgagcaggcg gttgattttt
    19141 accgccgccg ccgcccgcac gtcccgcccc atttgcgaac gccgcccgcg caattcgcgg
    19201 cgcagggcgc gtttttcctc gttcctcatt tcagacggcc tttcaggatt gcggtagaat
    19261 gttgcgatta taacgatttt gttaacattc aaacaggacg cacacaatgt ggcacatcgt
    19321 cgccatcggc tatctttttg ttgccgttat gtattccgcc gcgcagccga gtattgcgcg
    19381 cgctttgatt tatttggttt tttgggtggt tctgcccacc gtgttcacgg tcttcgccgt
    19441 aaccgtccgc cgccgcaacc gcctgatggg gcagcaggaa caggcagaat ccgaacagca
    19501 gcgcgcacaa cggcaaaaag acagcggcac aaaaccctga atcccttttc agacggcatc
    19561 ttatccgcta taatccgtca gttttccatt tcggaaacac actattttta aaacttatgc
    19621 ccactttcgc cgaagggtgc ttgacaatag gcgccgccta tcaagttcta tgcgattgaa
    19681 tgtgtgctct ttaacccttt caaggaaata acatgtctca aattactatg cgtcagatga
    19741 ttgaagccgg tgttcacttc ggtcaccaaa cccgtttctg gaacccgaaa atggctcaat
    19801 acattttcgg cgcgcgcaac aaaatccata tcgtaaactt ggaaaaaacc ctgccgatgt
    19861 tccaagaggc gcaagaagcc gtacgtcgtc tggttgccaa caaaggtacg gtattgttcg
    19921 taggtaccaa acgccaggca cgcgacatca tccgcgaaga agcgacccgc gccggtatgc
    19981 ctttcgtcga ccaccgctgg ttgggcggta tgctgaccaa ctacaaaacc gttaagcaat
    20041 ccatcaaacg cctggaagaa aaaaccgcag ccttggaaaa tgctgccgaa agcggtttca
    20101 gcaaaaaaga aattctggaa atgcaacgcg acgttgaaaa actggaacgc tctttgggcg
    20161 gtatcaaaaa catgaaaggc ctgcccgacg cgattttcgt tatcgatacc ggctaccaaa
    20221 aaggtactct ggttgaagct gaaaaactgg gcatccccgt tatcgccgta gtcgatacca
    20281 acaacagccc agacggcgtg aaatacgtca tccccggcaa cgacgactcc gccaaagcca
    20341 tccgcctgta ctgccgaggc atcgccgacg cagttttgga aggcaaaaac caagcgctgc
    20401 aagaaaccgt agccgctgcc caagaaaccg ctgccgagta attcggtaca cagaagaggg
    20461 gcgttatgcc ccttttctca aatatgccgt ctgaacgccc gttcgcggca cacgattccc
    20521 gaatgtggaa aatcctttcc gtatttccca aaaatctagg agattcaaaa tggcagaaat
    20581 tactgcaaaa atggttgccg acctgcgcgc cgctaccggc ctgggtatga tggaatgcaa
    20641 aaaagccttg gttgaagctg aaggcaactt cgacaaagcc gaagaaatcc tgcgtattaa
    20701 atccggtgcg aaagcgggca aactggctgg ccgtactgct gccgaaggcg tattggctta
    20761 tgcaatcaac ggtaatgtcg gcgcattggt cgaagtaaac tgcgaaaccg acttcgttgc
    20821 taaagacgcc ggcttcgtag aatttgccaa cttcgttgcg aaaactgctg ccgagaaaaa
    20881 accggcttct gttgaagaac tgagcgaact ggttgaatca gaacgcaaag ccatcatcgc
    20941 taaattgggc gagaacatgt ctgtccgccg cttccaagtg attgacactg ccaaccaact
    21001 ggttgcctac atccacggcg cattggcgac cgaaggcgtt ttggtcgagt acaaaggctc
    21061 tgaagatgta gcacgcaaaa tcggtatgca catcgttgcc gccaaaccgc aatgcgtaag
    21121 cgaagccgaa gtagatgctg aaaccgttga aaaagaacgc cacatctaca ccgagcaagc
    21181 catcgcttcc ggcaaacctg ccgacatcgc cgctaaaatg gttgaaggcc gtatccgcaa
    21241 attcttggct gaaatcactc tgaacggcca agcattcgtg atgaaccctg accaaactgt
    21301 tgcccaattc gctaaagaaa ac
//
LOCUS       DAXDSD010000027        20864 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXDSD010000027 DAXDSD010000000
VERSION     DAXDSD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20864
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.027"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(120..296)
                     /locus_tag="ACFB2Q_000796"
     CDS             complement(120..296)
                     /locus_tag="ACFB2Q_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917861.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            complement(299..1657)
                     /gene="murF"
                     /locus_tag="ACFB2Q_000797"
     CDS             complement(299..1657)
                     /gene="murF"
                     /locus_tag="ACFB2Q_000797"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC0917862.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1782..3428)
                     /locus_tag="ACFB2Q_000798"
     CDS             complement(1782..3428)
                     /locus_tag="ACFB2Q_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC0917863.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            complement(3451..4929)
                     /locus_tag="ACFB2Q_000799"
     CDS             complement(3451..4929)
                     /locus_tag="ACFB2Q_000799"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC0917864.1"
                     /translation="MFSKLTPLAETNIPPLLCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAVVNGAAFVFWDDDGKFAWNPEWKVPNQGIKNLKHRAGMLAAQVYDN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINTDDGYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGMEAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4954..6705)
                     /locus_tag="ACFB2Q_000800"
     CDS             complement(4954..6705)
                     /locus_tag="ACFB2Q_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC0917865.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVR
                     MHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPLSF
                     EKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNG
                     RYVDNKHVGTFIGFAPAKNPRVIVAVTIDEPTAHSYYGGVVAGPPFKKIMGGSLNILG
                     VSPTKPLTNVAAVKTPS"
     gene            complement(6766..7029)
                     /gene="ftsL"
                     /locus_tag="ACFB2Q_000801"
     CDS             complement(6766..7029)
                     /gene="ftsL"
                     /locus_tag="ACFB2Q_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC0917866.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRVQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(7056..8012)
                     /gene="rsmH"
                     /locus_tag="ACFB2Q_000802"
     CDS             complement(7056..8012)
                     /gene="rsmH"
                     /locus_tag="ACFB2Q_000802"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC0917867.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            complement(8009..8464)
                     /gene="mraZ"
                     /locus_tag="ACFB2Q_000803"
     CDS             complement(8009..8464)
                     /gene="mraZ"
                     /locus_tag="ACFB2Q_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC0917868.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8743..9990
                     /locus_tag="ACFB2Q_000804"
     CDS             8743..9990
                     /locus_tag="ACFB2Q_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC0917869.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAE
                     EIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQL
                     QELLPQTAGNIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSA
                     RPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKLQPSCRLNPLQTA"
     gene            10086..10162
                     /locus_tag="ACFB2Q_000805"
     tRNA            10086..10162
                     /locus_tag="ACFB2Q_000805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10120..10122,aa:Met,seq:cat)
     gene            10170..10245
                     /locus_tag="ACFB2Q_000806"
     tRNA            10170..10245
                     /locus_tag="ACFB2Q_000806"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10203..10205,aa:Ala,seq:ggc)
     gene            complement(10431..11252)
                     /locus_tag="ACFB2Q_000807"
     CDS             complement(10431..11252)
                     /locus_tag="ACFB2Q_000807"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC0917870.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMFWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(11285..11929)
                     /locus_tag="ACFB2Q_000808"
     CDS             complement(11285..11929)
                     /locus_tag="ACFB2Q_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC0917871.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQK
                     LTEQYGIDGTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11932..12795)
                     /gene="ftsN"
                     /locus_tag="ACFB2Q_000809"
     CDS             complement(11932..12795)
                     /gene="ftsN"
                     /locus_tag="ACFB2Q_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC0917872.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSEPDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKARKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSEVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(12901..14397)
                     /locus_tag="ACFB2Q_000810"
     CDS             complement(12901..14397)
                     /locus_tag="ACFB2Q_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC0917873.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHSARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14411..14731)
                     /locus_tag="ACFB2Q_000811"
     CDS             complement(14411..14731)
                     /locus_tag="ACFB2Q_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC0917874.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15219..16745
                     /gene="putP"
                     /locus_tag="ACFB2Q_000812"
     CDS             15219..16745
                     /gene="putP"
                     /locus_tag="ACFB2Q_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC0917875.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            16997..20602
                     /gene="putA"
                     /locus_tag="ACFB2Q_000813"
     CDS             16997..20602
                     /gene="putA"
                     /locus_tag="ACFB2Q_000813"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC0917876.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVKAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTGLAASGVQAVVEPDSPLASYTADLDGLLQISGKPENTGVNHVAALSP
                     LDSSRKQELANRDGALIRILPSGQGLDILQVFEEISCSVNTTAAGGNAGLMAVAD"
BASE COUNT         4877 a         6022 c         5119 g         4846 t
ORIGIN      
        1 atccgtactg tctgcggccc gccgccttgt cctgattttt gttaatccgc tatacatcgg
       61 gcttgcgccc gcttgaggtt tgcttgctgt ccggcaaggt ttcggacggc ttttgcagat
      121 taacgtgcag ggactttccc aatcacgcaa atcgggtagc tccccggatt tttgccgccg
      181 cccaagtcta aacataaatc ttcataaaga tacgcctgcg tccgcattcc ggcataaaac
      241 gcgccgacca gcgcggcaat cagcaataag gctttaaaaa aacgtcggct tttcattttc
      301 acttatcctc caatgcctcg accacttctt ccatctgcat aaagcgcgaa cctttcacca
      361 acacggtggc gcgttcgggc aaatcgtggc tcaacacttg aatcaacggg tctttggcgg
      421 cgaaccacaa accgtccgcg ccaaattttt ccgccgcttc gacgctgttg tcgccgacaa
      481 aataagccgc ttcgatgcct tggtcgcggg cgtacgcgcc gacttcggcg tgcatggcgg
      541 cggcttcgtc ctcgcccagt tcgcccatat cgcccatcac gaaaatgcgc ggcgcaggca
      601 tacgcgccaa cacgtcaacc gcggctttca tactgtcggg attcgcatta taagtatcgt
      661 caatcagggt tgcgcccttg atgccggctt tgacgttcag acggcctttg atgttgctga
      721 agccttgcaa accttccgcc acatcgttca aactcaaacc ggcagccaaa gccagcgcgg
      781 cggcagcggc ggcgttgtgg acattgtggc ggccgggaac aggcagcacc acggcagtgc
      841 gctcgtcgcc gcacaccaaa tcaaattcgc acgacaaagg tttcagcacg atattttccg
      901 cgcggacatc gccgctatcg acgccgaaag tgcacgtatt caaattaaac gttgccgttt
      961 tgaagacagc catattggca tcttcttgag gaatcagtgc catgccgtct gaacataagc
     1021 ctgcataaat ctcgcttttc gctttggcaa tatcgcccac tccgtcgaaa ccgcatccga
     1081 catgggcgcg cagggcgttg ttgaccaaag cggcatcggg tttggcgatt tgcgttaaaa
     1141 ccgccagttc gccaaaatgg ttcatgccca tttcaatcac ggcatagcgg tgtttttcgt
     1201 ttaatttcaa taaagtcagc ggcaatccga tgtggttgtt gaagttgcct gccgtcgccg
     1261 aaacggcatc atcgccgaaa cggcggcgca ataccgcagc cagcatctcc ttcaccgtcg
     1321 tcttgccgcc cgaaccggta atgccgaaca caaacgggtt cacattatcg cgccacgcct
     1381 tcgccaacgt ttgcaacgcg gcaagcgtgt catcgacttt caacgcgccg cccaaagccg
     1441 cgcaatcttc gcgcgaaacc acaaccgccg ccgcgcccgc agacaatacg cctccaacaa
     1501 aatcatgcgc gtcaaaccgc ccgcccgcca atgcgaaaaa cacatcgcct tcccgaatat
     1561 cgcggctgtc ggttacgatg cgcgacacgg gtttgttttc agacggcatc ggaagcttga
     1621 gggcttggca gatgaaattt aggtccagtg gtttcatatt tgctttcgtt aatattcggg
     1681 cggcggacac atcggtagcg gctgattttt ttatcgcctg ttttgctgtg gtaaaacaca
     1741 gattattttc ccattctcat tcggcatttt ttctgtacgt atcatttttt agacgtattt
     1801 ttagccgatt tgccttttcc cgcataccac ggcgcggggt cgtcggactg tctgtcgata
     1861 aaggcaaggt tattgccttc gcccggcaca tcggggacat tcccccaaaa atcatagccg
     1921 tcatcgggca actcgtcggt ttcgataccc gtccaactgc cgaatccgcg taaaaaatta
     1981 aacgcgctgc gcctcacttt aatcatttcg cgccgcgtgt catcggacga aattttaacc
     2041 agcggcacgc cgtagctttg acgcttccac tcgccgtgtg tcaacgttcg ctcgccgcct
     2101 ttgccgacat gcatcaaacc gtggtcggaa aagtaaacca aagaccagct ttctttattt
     2161 tcattcaata tcttaaccgt atcttctaaa aatttatcgg tttgcgcgat ggtggaaaca
     2221 tagcaggata ttttttcagt ttgatactga aaccgccgcg catccttatc caaacgtgtg
     2281 caaaaatcac tgtgcgaacc catcaggtgc atcacaatca gccgaggctt cgtgcctgca
     2341 tttcctgtca aaacccgttt gaacgccggc aacaaaaggc ggtcgctcaa tcccgcgctt
     2401 ttgccataat cacccctttg ggtaaaccac ggataatcgc tgcgtagggc ataggtggaa
     2461 atttcgttgg caaaatgccc caacattcct tgattagaca gccacgccgt ccgaaaacca
     2521 gcctgcttcg ccaagctgac aatgttattg ttcggttctc ccggcagccc caaagtctgc
     2581 ggcagcgaaa gattcgtcgc gtgggcggtc gattggtaac cgtttatcaa cagccctttg
     2641 gtccgactca aaaaaggcgt atcgggcaat gggaaaccgt aaacattcat ataatccgaa
     2701 cgcgcgctct caccgataac cacaacataa tttttatact tgggcgcaac atgacggata
     2761 tgccatgttg acgctttttt tgctgcttcc aaaatgtggg cgcgcttggc ggcatattgt
     2821 gccggcgcgg aagccaaatc gtaatacagg tcgaaaatat tcaacaacag gccggcatca
     2881 ggttctcgcc aatctttatc gccggcgatt ttctccatca ccgcgcagga taaaatcaaa
     2941 gtcaataata tggtcagcca tattttgctg cggcgtttat agttttttac gtcagcaaat
     3001 acccccacag atacacaata tttccaaact gtcaagccaa aaatgaaaaa taatgcctgc
     3061 acaaaataaa gcgacccggg aagattgccg acaaattcac gcgcctcggc aggattgctt
     3121 tccaatatcg aaccgactat ctgataagaa ggcgcaccat acagccagcc gaccggcaaa
     3181 tatagggcgg ttgtgccgac gtaaatcagt aaaacaactg aagaaacgcg cgggaagccg
     3241 cgtgccaata ataataaaaa tacaaccgaa gccaaagcgc ccacggcaga ataacggtag
     3301 ccgtattcat attccaagtg ataacctgat gcgatggccg cgccaaaaca aaaggctaaa
     3361 agcgcgcaaa tgcccaatgt attttttaat gtctgatcca tattttttat tcgggcggat
     3421 tgtttcatac gatgccgtcc gaatatccca ttatttacga gttaacaaag cctgcccgac
     3481 gatttcaaga tcggaaaaac ggtgcttcac gccctgtaca tcctgatagt tttcgtgtcc
     3541 tttgccggca atcaggatga tgtcgtttgc ggcggcctgc tcaaccgcat aacggatggc
     3601 ggcggcacgg tcggcttcga cgcattcggg cgcgggcacg gcgggcagga tgtcgttgat
     3661 gatgtcttgc ggattttcca aacgcgggtt gtcgctggtt acgacgactt tatccgcgcc
     3721 ctgtacggct gccgcgccca tcaatgggcg tttgccgcaa tcgcggttgc cgccgcaacc
     3781 gaatacgcac cataaggccg caccctgcgg tttgatttcc tgcaaagtgg agagtgcttt
     3841 ttccaatgcg tcgggcgtgt gggcgtaatc gacgacgacc aagggcttgc cgctgttcat
     3901 gatgcagtcc atgcgccctg aagcgggacg gatttttgcc agcacatcca ataccttatc
     3961 aagcggataa ccgttggcac aaagcaaggc gatgcaggcg gcgaggtttt gcgcgttgaa
     4021 ccgtccgagc aggcgggtgc ggcattttcc ttcgccccac ggggtttgga atactgcttc
     4081 catgccgtct gaagaggcgg taaagtcggt aatgcggatg tcggcgtgtt cgctgaaacc
     4141 atagctgtaa acggctaaat cgggacagtc ttttttcaga cgacctgcga gttccgcgcc
     4201 gtatccgtca tcggtattga taacggcgtg tttcaagccg tgccagtaaa acaggcgcga
     4261 cttgatagca ccgtaggctt ccatcgtgcc gtgatagtcg aggtggtctc gggtgaggtt
     4321 ggtaaagatt gcgctgcgga atggcacgcc gttgacgcgc gactggtcaa gaccgtggct
     4381 ggagacttcc atcgcggcgg ctgttgcgcc ctgttggcgg aaacggtaga gcagggtttg
     4441 gacatcgacg ggggcggggg tggtgtgcgt ggtttcttcc aatgcgcccc aaaagccgtt
     4501 gccgaccgtg ccgataatgg cggttttttc gcccaataaa tcggcagcct gcgccagcca
     4561 ttgtgtgatg gaggttttgc cgttggtgcc ggttacgccc cagactttga ggccgtctga
     4621 aacgttgtcg tacacttgcg ccgccaacat gccggcacgg tgtttcaaat ttttgatgcc
     4681 ttgattgggg actttccatt cgggattcca cgcgaatttg ccgtcgtcgt cccaaaaaac
     4741 aaaagccgcg ccgttgacaa cggcggcggg gatataactg cggccgtcgg tatattcgcc
     4801 ctgacaggca acgaaaatat caccttgttt gatttgacgg ctgtctgaat gcaacaaacg
     4861 ccctgctgcg tttgcacaca gcagaggcgg aatgttggtt tcagctaaag gggttaactt
     4921 gctgaacata aaataatctc gttgatactc ggattaagac ggtgttttga cggctgcaac
     4981 attggtcaaa ggtttggtcg gggaaacgcc caagatgttc aggctgccgc ccataatttt
     5041 tttgaagggc ggccctgcca ctacgccgcc gtaatagctg tgggcagtcg gttcgtcgat
     5101 ggttaccgcc acaatcacac gggggttttt ggcgggggca aaaccgataa acgtaccgac
     5161 gtgtttgttg tccacatagc ggccattgac cagtttgcgc gccgtaccgg ttttagcgcc
     5221 gacatcgaaa ccgtccaccg cacccgccgt accggtgccg cccggctcgg ttacggaaac
     5281 catcagattg cgtacctcgc gcgcggtcga ttctttgaat atgcgtttgc cttgcggcgc
     5341 aaccgcctgc ttctcaaagc tgagcggcag caaaacgccg tcgtgcgtca gtgcggtata
     5401 ggcgcgcgcc aattgcagca ggctcaattg caggccgtaa ccgaaagaca tcgtcgcctg
     5461 ttcgatgggc cgccacctgc gccaatttct caacaaacct gcagtttccc ccggaaagcc
     5521 cgagtgcata cgcacaccga tgcccaattc atgatagaag tcatacattt cttcggcgcc
     5581 gaaacgcgca gacagtttgc ttgtgccgac gttggacgat ttctgcataa tgccgcgcac
     5641 atccaaagag gggtaaacat gggtatcgtc gcgcacggga gacggtccga ttttataagg
     5701 ctgcgtattc agccgttcgt tcaaatcggt tttgcccgca tccaatgcct tcgcaatcac
     5761 gaacggtttg attgccgaac caggttcgat catatcggtt acggcacggt tgcgccgctg
     5821 ttcgctgtct gcccggccgg gtctgttggg atcgtaggcg ggcgtattgg ccaaggcgag
     5881 gatttccccc gtgcgggcat ccaaaaccac caccgttccg gcttttgcct gatggtattc
     5941 gaccgccttg ttcaactctt cataggccaa ggtctgaatc ctctgatcga gggaaaggat
     6001 gatgtctttg ccgttttgcg gtgctttatt gcgcggggag tccaagctgt ccacaatatt
     6061 gccctgccgg tcccgcaaaa caacttccgc gccgtcttcg ccatacaggc tgtcttcaag
     6121 cgaaagttcc aaaccttcct gacctttgcc gtcaatatcg gtaaatccga tgacgtgtgc
     6181 aaacaggttg cccatcgggt aatggcgttt taattctttt tcaaatacaa agttttccaa
     6241 acccaaggct ttgacctctt cggcaacctt gggatcgagc tgccgcttga tccaaataaa
     6301 cgacttgcct ttctgttcga gtttgttcct caaaacatcg accggcacat cgacaagctc
     6361 ggacaggcgt tccaattggg cggcagacgg catttccttc atatctttag gcacggcaaa
     6421 cagggactcc gtcggcgcgc tcaacgccaa aaccgcaccg ttccggtccg aaaccgtacc
     6481 gcgtgtagcc ggcaatgctt gagtccgcac aatccggttg tcgccctgtt ctttcaaaaa
     6541 gttatacgtt accgtctgca gatacagccc gcgggcaatc agacaggcaa acaagaccgc
     6601 cattgccatc aggacgaagc taatccgtcc gttactggtc atcggctttt tgacctgctc
     6661 ttctttgggc agcatccggg gcttatattc gcttttaatc aacattttta cttctcgtta
     6721 ttattatcct gatgcaggaa tccgattccg gcacacaggc tgcttctatc tttgatgttc
     6781 caccataaag gtattgcccg aaaccggcgg atggaggttt tgtttttctg ccgccgccct
     6841 gatcgcttcg tgattcgcca aacgcgcctg ttgcaccctc atttgcgcat aatcctgctc
     6901 caaggcgatt tcctgttttt tcgccttgtc caaagctgtg aagttgagcc tgtactggtt
     6961 ttgctgcatc acaacggaaa aagcggaaac gcacaccgca agcagcagaa agaaattcga
     7021 tttgttcatt gccgttcaga cgtttttctc tgttattatt ccggtatcgg accggcagtc
     7081 cgctccgcca cacgcaaaac tgcgctcctc gccctcgggt tggcggcaat ttccgcttca
     7141 cccggcttta atgccctgcc cacgattttc aggggcggct cgggcaaatc cgcttctctg
     7201 accgccgccc agctcggcag gggctcgtgt tgcgaatatt ttttgacaaa ctgcttcaca
     7261 atgcggtctt ccaacgaatg gaaagcaatg accgccaaac gcccgccctc tttcagacgg
     7321 cacatgacct gcggcaatac cgcccctact tcttcaagct cgcggttaat aaagatgcgg
     7381 attgcctgga aggtgcgcgt cgcaggatcc tgcccccgct cgcgagtacg gacgttttgt
     7441 gccacgatct gcgccagctt gcgggttgta tcgattggac tttccgcccg ttgcgcgaca
     7501 atggcgcgca caatctggcg gctaaaccgc tcttcaccat aattcttgat tacctcgtgc
     7561 aaatcctgtt ccgacgcaac cgctatccac tctgcggcag acataccgcg cgtcgtatcc
     7621 atacgcatat ccaaaggggc atcgaaacgg aagctgaaac cgcggctgcc gtcatcgatt
     7681 tgcggagacg aaatccccaa atcaaacagc gcaccgtcca ccttgccgat acccaaaccg
     7741 tccaatgccg tctgaaacga agcaaaaccg ccatgcacga caccgacccg tttgtccgaa
     7801 cgcgccagct cttctgccac agcaatcgcc tgcgggtctt tgtcgaaaac aatcaaccgc
     7861 cccgcatcgc ccaaacgcga caaaatcagc cgggaatgcc ctcccctgcc gaacgtaccg
     7921 tccacataga caccgtcttc gcgcacggca agcgcatcca ccgcctcatt cagcaagacc
     7981 gtgatatgcc ggtaactttc tgctccactc acaattgcaa atccgtctga ctcaactgga
     8041 aggcaagttc gtcaggatcg tcatccaaag cctgaaccat ctcagcctcc cactgctcgc
     8101 gaccccaaag ctccaaacgg ttggcacggc cgaccaaaac aacttcacgg tcgaaatcca
     8161 cccttttcct cagtccggca ggaaccagca ccctgccggc gctgtcccat tccaaaattt
     8221 ccgcgttatg cagcaaaaga ttttgaaacc gccgcaaaac agggttatcc gccactttta
     8281 agtttaaaag ttgcgccgca accttttccc actccgcaac agggtacatc aacagcttat
     8341 gtttcgactc gagcgttgcc actacggcag gcgtatagag gcgcgacaga atgtcgcgga
     8401 atttggcagg aacagccaac cgccccttac tgtcgatgct taattcgtgt gcgccgccga
     8461 acatgacatg tcccaagccg aaatcaaaat cacaagggta aaagagacac tttgccccac
     8521 aattccccac caatcgacac tataagaaat tttaaacact cggtcaaatc agggcatgaa
     8581 aactcattaa catatctgaa atttttattc tgtttaaaaa caataagata aaaaatgacg
     8641 acaacggcac ggcgggtgca gtacagagtg accgaaccaa taaacaacta tatatgatta
     8701 atttaataat ataaacacaa tatatagtat taagataaag ccatgacagc acccgtacca
     8761 acgtgtaata tgtcgggaaa tccaataaat ttacacaagc taacatttat catgctcctc
     8821 ccctcccccg aagcacggca atcctcgctc aatctgcaaa ccctcattgc cgaagaaatc
     8881 ggcaaacacg gcaattggat tccattttca cgttttatgg aattggtttt atacgctccg
     8941 caatacggct actacaccgg cggcagccat aaaatcggca ataccgggga ttttattacc
     9001 gcaccgaccc tcacccctct gtttgcacag acactggcac gccaacttca agaactcctg
     9061 ccccaaacgg cgggcaatat ctatgaattc ggcgcgggaa ccggacagct tgccgccgat
     9121 ttgttgggca gcgtttcaga cagcatcaac tgttactata ttattgaaat atcgccggag
     9181 ctggcagcac gtcagaaaaa cctgattcaa gcccgtgcac cggaagcatc tcaaaaagtt
     9241 gtccacttga ccgcacttcc cgaagcgttt gacggcatca tcatcggcaa cgaattactc
     9301 gatgccatac ctgtcgaaat cgtccgtaaa aatgaaggag gattactcga gcacatcggc
     9361 gtttgcacgg ataacggccg tttcgcctat tctgcaagac ccttgcacga cccgtcgctt
     9421 tccacctcgg cttccctcta ttttcctcaa acagattatc cctataccag cgaactgcac
     9481 ccgcaacaat atgcctttat ccgcaccctt gcctcaaggc ttgaacgcgg gggtatgata
     9541 ttcatcgact acggttttga tgcggcgcag tattaccacc cgcaacgcaa tcaaggcaca
     9601 ttgatcggac actaccgaca ccacgttatc cacaatcctt tcgacttcat cgggttggca
     9661 gatctgaccg cccacgtcaa ctttaccgac attgcccaag ccggtacgga tgcaggactg
     9721 gatttgacag gctatctgcc ccagtcccat tttctgctga atctgggcat aaccgaactg
     9781 ttggcacaga cgggtaaaac agattcggca gcctatatcc gtgaagccgc cgctgttcaa
     9841 aaactgattg accagcatga aatgggcgaa ctgtttaaag tcatcgcatt cggaaaaaat
     9901 atcggcatcg actgggcagg attccgcttc ggcgacatct gccacaaact ccaaccctcg
     9961 tgccgtctga atccgcttca gacggcataa actttttaac atttaaaaac aagaaactaa
    10021 ttcaaaatta aaaaatacgg cttgtcaaaa aaacaaaaaa acatataata gctaattcac
    10081 gaaacggcga attagctcag tcggttagag cagaggaatc ataatccttg tgtccggggt
    10141 tcgagtccct gattcgctac cagattttcg ggggtatagc tcagttggta gagcgcttgc
    10201 atggcatgca agaggtcagc ggttcgatcc cgcttacctc caccagataa aaaagcacag
    10261 accgtaaaag gtatgtgctt ttttattgcc tgattgccgg aaaataaaga ataaaccact
    10321 gccttcaaaa caggcaatcg actttaaacc tattgccccg cctgtcctga ttttatcagc
    10381 ccgccagaca aacccgacct gaataatgtc ttcaggtcgg gtttatggtt tcattcccaa
    10441 cttatccagc ccgacaacca caatattatg atgacaatgc cgaaaacaat gcggtaatag
    10501 gcaaacggga tatagttttt cttggaaaca aacttcagca gtgctttaac cgctaccaaa
    10561 ccggaaacaa aagcggcaat aaagcctatc aaaatcaaac cgacatcatg cagggtgaaa
    10621 aatcggtaat gtttcaggac atcataagcc gttgctgcaa ccatcatcgg aacggccaag
    10681 aaaaatgaaa actccgttgc cgttttccgc tcgattcccc aaaacatccc gcccataacc
    10741 gtactgcccg aacgggacgt acccggaacc agtgcaaaca cttgggcaac accgatcatc
    10801 aacgcatcaa tcggacgcaa tgcatcaaca tcggcaattt taggctctgc tcggctttgg
    10861 cgtttctcca cccacaaaat aaaaaaaccg cccaaaacca gcatgactgc aacactcaag
    10921 gggttaaaca gatactcttt gatttgtttg tcgaacaaca gccccatcac ggcggcaggt
    10981 ataaaagcaa tggcaagatt gaggacgaaa cggttggctt tccggtcttt tcccacgcca
    11041 tgcaacacat tgctgaaacg ctgccggtat tcaaacacta ccgccaaaac cgcaccgagc
    11101 tggatggcaa tttcaaaaac cttgtgattg ctgtgaaaac caatcagatt gccgaacaca
    11161 atcaaatgtc cggtgctgga aatcggtaaa aattcggtaa aaccttctac caagcccatc
    11221 atcagggctt tcaggacaat cagaaaatcc attgcttgcg cttctttcgg atacgggagt
    11281 tcggctattt ctgtacagca ggggtctgac gcttgcgttc ttccctgact ttggcaacca
    11341 attctttaat cgtatgaacg ccgccatcaa agccattatt gaagataacg cggtattttc
    11401 cgccgacaat aacggtcggc gtgccgtcaa taccgtattg ttccgtcagt ttctgcattt
    11461 ttaatgcgac ggcggcagct tcgggggaat cataggcgcg catcagtttt ttgccgtcaa
    11521 aacctttttg agataaagcc cattttccgg caacagccct gttttccaaa cggatttttt
    11581 gttcgtaaac tgctttaaac acagcagagt ttgcctgata tttcaaaccc gacagcttga
    11641 ccgcagcagc cattcttgcc agaccgagca tttcaggccg ccagaccacg tgctccgtcc
    11701 gcagataagt atcagacggc aatgccttgc ccagtttcaa taacaaagga tcgaaatgat
    11761 ggcaatgtac gcaaaaatag ccgaaaaatt ccaaaacctc aatttttccc ggctgttctt
    11821 gaggaatggg tttatccaac acaagatagt cttccccttc cgtcagggca tatgcctgcg
    11881 cggacaacac tgccgacagc agcagcggca acagatgttt aaacttcata attatttgcc
    11941 ttcaatcgca cggatcaggc tggcaacccc atgctttttc aactcgtcct gcattttttt
    12001 caccgcatcg gcagacatat tgccgctttg cacgcggtaa agcgttttat gtcccgcctg
    12061 atagccgacc acttcggaag atatgcccaa gattgccagt ttggcacgct gcccttccgc
    12121 gctccggcgg tcggcatacg cgcccatttg cagataatgc gttgcttccg ccttgccaaa
    12181 cgttttcatt ttctgcactt ctttggcagc ggcactgcgc gctttttcga tgctgccgct
    12241 gttgaggatt tgttccgggg tcggtttggg tgcaactttt tccttcgccg cctttttctc
    12301 ttcttttgaa gcttttttct ctgtttcttt agacggtttt accgcttgtt ttttaaccgt
    12361 ttcggcatct ttcttccgcg ctttttccct gacggtttgt tcacgctctt ccgtcagtgc
    12421 tttcttgcgc actgcctgtc cgtccggctc ttcccgttcc ggctcgcccg ccttttcttc
    12481 aacctcgtcg gctttgtcgg caacgggctg cttgtcggca gctttttccg catccgactg
    12541 ctctgcctct ttcgcaacat ccggttcgga caaggcgttt tgatcggccg gttcaggttg
    12601 gatgtcttcc ttaggctggt ttttcagttt caggatttcc gtttctgcag gctgcttcga
    12661 cggagccggg attttgaacg cattttgacc gccctggttc agataaagca aaataccggc
    12721 aataatgacc gttgccagta tcaaaccgaa gaagaaaccg gacagacctt ttccggattg
    12781 ggaaaatttg ttcataaaca taccttaatg tgtttcagac ggcattaacg ccgtttgctt
    12841 acgggcggat attctaacaa tatcgccata tttgggcaaa acctgcttcc attcccgttc
    12901 ctataaagca cgacggaagc ctatggcttt catgacgtgg cttctgccga cttcttcatc
    12961 gcccgccaaa tccgccaatg tacgcgccac gcgcataatg cggtggaagc tgcgggcgga
    13021 aagagacaat ttttccagca ggctacccaa ggcttcctgc gcttcttttt ggatgcaggc
    13081 ttgggaatcg agttcgctga cgctcaaagc ggcgttgact ttgccctgcc gtgcgtattg
    13141 tttgccgcgg gcggcgatga cgcgttccaa aacggacgca ctgctttcgc ccgcttcctg
    13201 ctgcatcagt tcggcggcgg acaggctggg gacttcgatg gtcaaatcga tgcggtcgag
    13261 cagcggaccg gaaattttgc ttcggtaacg cgcgacactt tcgggcgtgc agcggcaggg
    13321 tttgacggga tgcccgagat aaccgcacgg acacgggttc atggcagcga caagctggaa
    13381 tttggcggga tagacggctt ggcgcgccgc gcgggaaatg tggatttcgc cgttttccaa
    13441 cggttcgcgc aaaacctcca aaactttgcg gtcgaactcg ggcaattcgt ccaaaaataa
    13501 aacgccgtga tgcgccaatg aaatctcgcc cggacgcgga tccgagccgc cgccgaccat
    13561 agccgccgcg ctggcgctgt gatgcggact gcggaaagga cggttgctgt cgagttgttg
    13621 ttggtggttg ggcaggagcg aacgcaatgc ccaaacttct accaattcgt cttcggccag
    13681 cggcggcagg atgccgggca gccgttggga gagcatagac ttgcctgttc ccggcgggcc
    13741 catcatcaag aggctgtgtc cgcctgcggc ggcgatttcc aaagcaaggc gggcagagtg
    13801 ctgacctttc acatcacaca aatcaggttg tccgccatgt tcagacggca tttgcggtac
    13861 ggaacattcg gtttgcgcca aaggttcgat accgttcaaa tgagcggcga cttcgcccaa
    13921 agagcgcgcg ccgtaaacgg taatgccgcg catcacggcg gcttgtcctg cgttttcttc
    13981 aggcaaaaca aatgcacgtt ttgcctgcat accctgccac gccatcgcca acgcgccgcg
    14041 cacggggcgc aacaaccccg acagtgccaa ttcccccgca aactcgtatt ccgccagctt
    14101 ttcgggtgca acctgccccg atgcggcaag gatgccgatt gcaatcggca aatcgaaacg
    14161 ccccgactct ttgggcaggt cggcaggggc gaggttgacg gtgatttttt tggcggggaa
    14221 ttcaaaaccg ctttgaataa tggcggcacg gacgcggtcg cggctttcct ttacttccgt
    14281 atcgggcagt ccgacgatgt tgaaatgcgg caggccgttg gcaaggtggg cttccacttc
    14341 gaccaacggc gcattcatac cgctcaaggc gcggctgtaa accaaggcaa gcgacatatt
    14401 tcagacggcc ttattcgccg gcttcggttt gctgcctgat ttcggcgacg gcttcttcgg
    14461 cagcggcttc agccgcttcc aatgctgccc gttcgggatt ttgcgcggct tcgagtttcg
    14521 ccagacgtgc ttccaaagcc gccagtttgg tacgggtttt aatcaaaacc tgctgctgga
    14581 tgtcgaattc ttcgcgcgta accaaatcca tacggttgaa cgcgccgccc agcatcgcct
    14641 taatattttt ttccacatct ttggcagggc tgttggcgat ggtttcgctg attttcgagc
    14701 cgacttcttc aaaaagctgc ttgccgaaca taatctgcat ccttcctgaa catatcaaat
    14761 tcaagcggct attgtataag gaaaaatgcc gtctgaaaac gggcggcgga taatcgtcaa
    14821 aacataccgc gccccctttg cggttgctaa aattacactt cgagacacct gccgcctgaa
    14881 aacggatttc atattccgcg caacgcccga ttacattaca agacactaca aaacaatatg
    14941 ctgttttaaa tgatttttcc gatgcgcatc gtttcaaact cggcttttta agccattaag
    15001 tgctttgcaa acaacagaaa ttgggttatg ctgaaacgga ttatttacaa tttcatatag
    15061 tttcattaca tatcttattg taattgaaga taatttatcc gaatcccctt tcgggtatcc
    15121 ggattttccg ttgtactttc attagaaaaa catttcaggc gcaagttgct tgcaatttca
    15181 aagcctgtaa ttctgaagtt ttagacgagg aatagcacat gaatcccatg tacatcactt
    15241 ttgcaatcta tttggttgca gtccttctca tcgggcttgc cgcttatttt tccacgcgca
    15301 atttcgatga ttatattttg ggcgggcgca gcctgggccc gtttgttacg gcgatgtcgg
    15361 caggcgcgtc cgatatgtcc ggctggcttt tgatgggtct gccgggcgcg atttatttga
    15421 gcggtttgaa tgaggcttgg attgccatcg gcctcttggt cggcgcgtat ttcaactggc
    15481 tcttggtggc gggccgcctg cgcgtgcata ccgaatatgc caacaatgcg ctgacgctgc
    15541 cggattattt cttccaccgc ttcggcgcgg gcggacactt gatgaaagtg gtttccgcac
    15601 tgattatcct gtttttcttc acgatttatt gcgcctcggg cattgtggcg ggcgcaaccc
    15661 tgttccaaag cctgtttgaa ggtatgactt acaatcaggc aatgtggctg ggcgcgggcg
    15721 cgaccatcgc ctataccttc ttgggcggct tcttggcggt cagctggacg gatacgctgc
    15781 aggcttcttt gatgattttc gccctgattt taacgccggt gatggtctat ctgggcttgg
    15841 gcggcgcgga acagatgtct gccgcgattc aaagcgtcgc cgcaggcacg ggcaaagaat
    15901 acggcagcct gtttgccggt acgaccgtca tcggcatcat ttccaccgcc gcctggggct
    15961 tgggctattt cggacagccg cacattttgg cgcgctttat ggccgccgaa agcgcgaaat
    16021 cgctggtatc cgcacgccgc atcggcatga cttggatggc gttgtgcctt gcgggcgcgg
    16081 tagcggtcgg ttatttcggc attgcgtatt tcggtgcaaa ccccgacaaa gtttcttcta
    16141 tgagcggcaa ccacgaacgc atcttcatcg cgctttccac cctgctgttc aacccttgga
    16201 tcgccggcat cattttgagc gcgattttgg cggcggtgat gtccaccctg tcctgccagc
    16261 ttttggtttg ctccagcgcg attaccgaag acttctacaa aggctttttg cgtaaaaacg
    16321 cgcaacagtc ggaattggta tgggtcggcc gcctgatggt attggcgatt gccgtgattt
    16381 ccatcctgat tgcttccgat ccgaacagca aagtattggg actggtgtct tacgcgtggg
    16441 caggtttcgg tgcggcattc ggcccgattg tgattctgtc cgtattgtgg aaacgcatca
    16501 ctgcctacgg cgcgttgtca ggcatggttg caggtgcgtc aaccgttgtt gtgtgggcgg
    16561 aatgggtaaa aaaaccggct cgagcggcag gcgaaagcgg cttgttgacg atgtacgaaa
    16621 tcgtcccggg ctttattgtt tgcttgatcg ttgccgtgtt ggtttccctg ttcaacaaag
    16681 aaccttcgcg cgaaatccaa gaacgctttg aaaaagccga cgcagattac cgcgccgccc
    16741 gataaacccc tttcagacgg cacggcatcc cggttcgtgc cgtctgaacc cgcttccggg
    16801 ccaaacacaa ataaaccttg cctgcataca ggccttattt gtgttttacc cgtttcattt
    16861 tcaaagaaac ggaagcaccg attctgcccg catttgaagc cttgtatttc atctgcggat
    16921 ttattacgat attaccgtat tcaggccgca ccgatgccgt ctgaaccccc cgaaaaaact
    16981 ttggaggatc caaaaaatgt ttcattttgc atttccggca caaactgccc tgcgccaagc
    17041 gataaccgat gcctaccgcc gtaatgaaat cgaagccgta caggatatgt tgcaacgtgc
    17101 acagatgagt gacgaagagc gcaacgccgc ctccgagctt gcccgccgtt tggttaccca
    17161 agtccgcgcc ggccgcacca aagccggcgg cgtggatgcg ctgatgcacg agttttcact
    17221 ctccagcgaa gaaggcatcg cgctgatgtg tctggcagaa gccctgctgc gtatccccga
    17281 caacgccacg cgcgaccgcc tgattgccga caagatttca gacggcaact ggaaaagcca
    17341 tttgaacaac agcccttccc tcttcgtcaa tgctgccgcc tggggtctgc tgattaccgg
    17401 caagctgacc gccacaaacg acaaacaaat gagttccgca ctcggccgcc tgatcggcaa
    17461 aggcggcgca ccgctcatcc gccaaggcgt aaattacgcc atgcggcttc tgggcaaaca
    17521 gttcgtcacc ggacagacca ttgaagaagc cctgcaaaac ggcaaagaac gcgaaaaaat
    17581 gggctaccgc ttctccttcg atatgttggg cgaagccgcc tacacccaag ccgatgccga
    17641 ccgctactac cgcgactatg tcaaagccat ccacgccatc ggcaaagatg cggcaggaca
    17701 aggcgtttac gaaggcaacg gcatttccgt caaactctcc gccatccatc cgcgctactc
    17761 gcgcgcccaa cacggccgcg tgatgggcga actgttgcct cgcctgaaag aattgttcct
    17821 tttgggtaaa aaatacgata tcggtatcaa catcgatgcc gaagaagcca accgtctgga
    17881 gctgtctttg gatttgatgg aggcactcgt ttccgacccc gatttggccg gctacaaagg
    17941 catcggtttc gttgttcaag cctaccaaaa acgctgcccg ttcgtcatcg actatctgat
    18001 tgacctggcc cgccgcaaca accaaaaact gatgatccgc ctcgtcaaag gcgcgtattg
    18061 ggacagcgaa atcaaatggg cgcaagtgga cggcttgaac ggctatccga cctacacccg
    18121 caaagtgcat accgacatct cctacctcgc ctgcgcgcgc aaactgcttt ccgcgcaaga
    18181 cgcggtattc ccgcaattcg ccacccacaa cgcctacacc ttgggcgcaa tctaccaaat
    18241 gggcaaaggc aaagattttg aacaccaatg cctgcacggt atgggcgaaa ccttgtacga
    18301 ccaagtcgtc ggtccgcaaa acttaggccg ccgcgtgcgc gtgtacgccc cggtcggcac
    18361 acacgaaacc ctgctcgcct acttggtgcg ccgcctgttg gaaaacggcg cgaactcgtc
    18421 tttcgtcaac caaatcgttg atgaaaacat cagcatcgac cgcctgatta agagcccgtt
    18481 cgacaccatc gccgaacaag gcatccacct gcacaacgcc ctgccgctgc cgcgcgattt
    18541 gtacggcaaa tgccgtctga actcgcaagg cgtggacttg agcaacgaaa acgtattgca
    18601 gcagcttcaa gaacagatga acaaagccgc cgcacaagac ttccacgccg catccatcgt
    18661 caacggcaaa gcccgcgatg tcggcgaagc gcaaccgatt aaaaaccctg ccgaccacgg
    18721 cgatgttgtc ggtacggtca gctttgccga tgccgcgctt gcccaagaag cggttggcgc
    18781 agccgttgcc gcgttccccg aatggagtgc gacacctgcc gccgaacgcg ccgcctgcct
    18841 gcgccgtttc gccgacctgc tcgaacagca cacccccgcg ctgatgatgc ttgccgtgcg
    18901 cgaagcgggc aaaacgctga acaacgccat tgccgaagtg cgcgaagccg tcgatttctg
    18961 ccgctactac gcaaacgaag ccgaacacac cctgcctcaa gacgcaaaag ccgtcggcgc
    19021 gattgtcgcc atcagcccgt ggaacttccc gctcgccatc tttaccggcg aagtcgtgtc
    19081 cgccctggcg gcaggcaaca ccgtcatcgc caaacctgcc gaacaaacca gcctgattgc
    19141 cggttatgcc gtttccctca tgcacgaagc cggcatcccg acttccgccc tgcaactcat
    19201 cctcggcgca ggcgacacgg gtgcggcatt gaccaacgat gccagcatcg gcggcgtgat
    19261 tttcacaggc tcgaccgaag tggcgcgcct gatcaacaaa gcccttgcca aacgcggcga
    19321 caatcccgtc ctgattgccg aaaccggcgg acaaaacgcc atgattgtcg attccaccgc
    19381 acttgccgaa caagtctgcg ccgacgtatt gaactccgcc ttcgacagcg cgggacaacg
    19441 ctgctccgcc ctgcgtatct tatgcgtcca agaagacgtt gccgaccgta tgctcgacat
    19501 gatcaaaggc gcgatggacg aactcgtcgt cggcaaaccg attcagctca ctaccgatgt
    19561 cggccccgtc atcgatgccg aagcgcagca aaacctgttg aaccacatca acaaaatgaa
    19621 aggcgttgcc aagtcttacc acgaagtcaa agccgccgcc gatgtcgatt ccgaaaaatc
    19681 cacgttcgtt cgccccatcc tgtttgaatt gaacaacctc aacgaactgc aacgcgaagt
    19741 cttcggcccc gtcctgcacg tcgtccgcta ccgcgccgac gaactcgaca gcgtcatcga
    19801 ccaaatcaac agcaaaggct acgccctgac ccacggcgta cacagccgca tcgaaggcac
    19861 ggtacgccac atccgcagcc gcatcgaagc cggcaacgtt tacgtcaacc gcaacatcgt
    19921 cggtgcagtc gtcggcgtac agcccttcgg cggacacggt ctgtccggca caggccccaa
    19981 agcaggaggt tcgttctacc tgcaaaaact gacccgcatc cccgaatggg ttgccccgac
    20041 cctgagccaa atcggacagg cagacgaagc cgcgctcaaa cgcctcgaag ccctgattca
    20101 caaactgccg ttcaacgccg aagagaaaaa agccgcagcg gccgctttgg gacacgcccg
    20161 catccgcacc ctgcgccgtg ccgaaaccgt ccttaccggc ccgaccggcg agcgcaacag
    20221 catctcatgg cacgcgccaa aacgcgtttg gatacacggc ggcagcacgg ttcaagcctt
    20281 tgccgcactg accggacttg ccgcctccgg cgtacaggca gtggtcgaac ccgacagccc
    20341 gctggcttcc tacaccgccg acttggacgg tttgctgcaa atcagcggca aacccgaaaa
    20401 caccggtgtg aaccacgtcg ccgccctaag cccactggac agcagccgca agcaggaact
    20461 ggcaaaccgc gacggcgcac tcatccgcat cctcccgtcc ggacaaggct tggacatcct
    20521 gcaagtgttt gaagaaattt cttgcagcgt caacaccaca gccgccggcg gcaacgccgg
    20581 cctgatggcg gttgccgact gattttgcca aaccgcccgg gcgcggcccg tgaacaaatg
    20641 ccgtctgaaa acctttcagg cggcatttta tagtggatta acaaaaatca ggacaaggcg
    20701 gcgagccgca gacagtacaa atagtacggc aaggcgggcc aacgctgtac tggtttaaat
    20761 tcaattcact atatgtgttg gcacatcgct ccaaacctga tataatccgc ctttcaacat
    20821 cagtgaaaat cgttcttttt agtcagttaa cattaaattt cgga
//
LOCUS       DAXDSD010000028        20797 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXDSD010000028 DAXDSD010000000
VERSION     DAXDSD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20797
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.028"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..347)
                     /locus_tag="ACFB2Q_000814"
     CDS             complement(<1..347)
                     /locus_tag="ACFB2Q_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFC0917877.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLIS"
     gene            679..891
                     /locus_tag="ACFB2Q_000815"
     CDS             679..891
                     /locus_tag="ACFB2Q_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917878.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
     gene            complement(984..1616)
                     /gene="mtrR"
                     /locus_tag="ACFB2Q_000816"
     CDS             complement(984..1616)
                     /gene="mtrR"
                     /locus_tag="ACFB2Q_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC0917879.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAADAEGGSWTVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGESFDLGKTAPRIIGIMMDNLENHPCLRRK"
     gene            1866..3104
                     /gene="mtrC"
                     /locus_tag="ACFB2Q_000817"
     CDS             1866..3104
                     /gene="mtrC"
                     /locus_tag="ACFB2Q_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC0917880.1"
                     /translation="MAFYASKAMRAAALAAAVALALSSCGKGRDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVSADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGAIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVSNDQNILMPGLYVRVLMDQVAADNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWAPSENQA
                     AAPQAGVQTASEAKPASEAK"
     gene            3116..6319
                     /gene="mtrD"
                     /locus_tag="ACFB2Q_000818"
     CDS             3116..6319
                     /gene="mtrD"
                     /locus_tag="ACFB2Q_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC0917881.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHAIYPGASAQVMEGSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNV
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNIQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNIYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERLAVLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMTLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGAAGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTASGSDAVAVAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAAGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVRHASKAGITGSDDKQY"
     gene            6372..7775
                     /gene="mtrE"
                     /locus_tag="ACFB2Q_000819"
     CDS             6372..7775
                     /gene="mtrE"
                     /locus_tag="ACFB2Q_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC0917882.1"
                     /translation="MNTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFQNDTSV
                     SSVRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASVANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINRPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSVELGGLFKSGTGVWA
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTGK"
     gene            complement(7976..8168)
                     /locus_tag="ACFB2Q_000820"
                     /pseudo
     CDS             complement(7976..8168)
                     /locus_tag="ACFB2Q_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8174..8380)
                     /locus_tag="ACFB2Q_000821"
                     /pseudo
     CDS             complement(8174..8380)
                     /locus_tag="ACFB2Q_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            8730..9509
                     /locus_tag="ACFB2Q_000822"
     CDS             8730..9509
                     /locus_tag="ACFB2Q_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC0917883.1"
                     /translation="MKLVRSQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            complement(9547..9653)
                     /locus_tag="ACFB2Q_000823"
                     /pseudo
     CDS             complement(9547..9653)
                     /locus_tag="ACFB2Q_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(9748..11082)
                     /gene="gdhA"
                     /locus_tag="ACFB2Q_000824"
     CDS             complement(9748..11082)
                     /gene="gdhA"
                     /locus_tag="ACFB2Q_000824"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC0917884.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWA
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
     gene            11506..12300
                     /locus_tag="ACFB2Q_000825"
     CDS             11506..12300
                     /locus_tag="ACFB2Q_000825"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC0917885.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
     gene            12309..13321
                     /locus_tag="ACFB2Q_000826"
                     /pseudo
     CDS             12309..13321
                     /locus_tag="ACFB2Q_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703756.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(13496..14833)
                     /locus_tag="ACFB2Q_000827"
     CDS             complement(13496..14833)
                     /locus_tag="ACFB2Q_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC0917886.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PNQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLGIL"
     gene            complement(15067..16131)
                     /locus_tag="ACFB2Q_000828"
     CDS             complement(15067..16131)
                     /locus_tag="ACFB2Q_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC0917887.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(16132..16890)
                     /locus_tag="ACFB2Q_000829"
     CDS             complement(16132..16890)
                     /locus_tag="ACFB2Q_000829"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC0917888.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(16965..17040)
                     /locus_tag="ACFB2Q_000830"
     tRNA            complement(16965..17040)
                     /locus_tag="ACFB2Q_000830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17005..17007),aa:Ile,seq:cat)
     gene            complement(17094..18439)
                     /locus_tag="ACFB2Q_000831"
                     /pseudo
     CDS             complement(17094..18439)
                     /locus_tag="ACFB2Q_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757555.1,RefSeq:WP_003777802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase"
     gene            complement(18433..19162)
                     /gene="fabG"
                     /locus_tag="ACFB2Q_000832"
                     /pseudo
     CDS             complement(18433..19162)
                     /gene="fabG"
                     /locus_tag="ACFB2Q_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
     gene            complement(19200..19628)
                     /locus_tag="ACFB2Q_000833"
                     /pseudo
     CDS             complement(19200..19628)
                     /locus_tag="ACFB2Q_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioester dehydrase"
     gene            19828..20571
                     /locus_tag="ACFB2Q_000834"
                     /pseudo
     CDS             19828..20571
                     /locus_tag="ACFB2Q_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            20574..20657
                     /locus_tag="ACFB2Q_000835"
                     /pseudo
     CDS             20574..20657
                     /locus_tag="ACFB2Q_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
BASE COUNT         4767 a         5193 c         5817 g         5020 t
ORIGIN      
        1 gaaatcaagc ccgatttttc cgcaatcccc acgcccaata aagaaaccaa caccgttccc
       61 aacggcgcga aaccggtgaa atttttaacg gtatgcgtca ggattttgat caaaccgtcg
      121 gcatcgagca ggctgacaac gtgaatcaaa ccgtcatcgg cacgtccttt cgccccaaca
      181 ggacgcggat cggggacgga tagtccgaaa tacgcaccga cggcagaggc aatcagcaat
      241 aacacaatga aaataataaa aagcgtaacc gggtgcggca acatattgcc cagccattcg
      301 actgtgcgta aaaatcgtcc gctacgacgc gcgtcggttt gactcatctg cttctcctta
      361 aaaaaatata ttgagtgcat taaatttttg aatttaacat gaagtgtttt gtaagcaaac
      421 taaaataatc gtatatgtat ataaccgtaa tagattaaaa cggaaaatgc ccacatcgtt
      481 attctcataa aggcggtaat ccgaaaactt gacgttaaag ccttatcagg aatgactgga
      541 actcaaaaaa acgaattccc gcctgcgcgg gaatgacggg attttcggtt tttgtttgca
      601 agaatgccgg agaaaaatgc agtttacatt cgaatggtat tgttattaat tgtaaataaa
      661 tattggaagg taaatgatat gaaaaatgaa attcaaaaaa tcatggataa atatgatcca
      721 tggcatgaag atgattttga gtcgtatgaa gacattgcca aagatgtatc gttgatgaca
      781 gataaaacgt tcattgaaca ttatttgtta gaagtttatt cagaagaaaa cggacatttt
      841 gaccaagaga atatccatgc gatgataggg gaaattaaaa atgcaattta gtgaatatgt
      901 atttttagaa gaatgctttg tgtcattttg atgccgtttg aaagggtttg ttcgtttcag
      961 acggcattgt taccaaggct tgattatttc cggcgcaggc agggatggtt ttccaagttg
     1021 tccatcatta tcccgatgat gcgcggggcg gttttgccca aatcgaaact ttcgccggaa
     1081 gagaaccaac gccaaatcag cccgtccaac gtcgatttga tgaagatgac cgccgtttcc
     1141 ttgtccaaat cgtcagccaa atcctgattt tccaccgctt cggtcaaaac ggcggtaatt
     1201 ttctcgcgcc agattgcctg atgcttgcgg gcaatggcga taacggcggc gttttgctcc
     1261 gtgtgttcgc attttaaaaa caggatgttg tggaatttgt agtagatgtc gttgctttgc
     1321 agccgctcga aaaagtgcag cagcgtgtgg cggaataccg tccaagaacc tccttcggca
     1381 tctgcggcat cttgcgcgat gcagttttcg atgtcgtcgc agatacgttg gaacaacgcg
     1441 tcaaacaagt cttccttatt tttgaaatgc caatagagcg cgccgcgcgt tacgccggcg
     1501 gcttgggcga tttcgttgag cgaggtgcgg gcaatccctt tgcggtaaaa ggtttccaag
     1561 gcggcaagca tcaggtgttc tttggttttc aaggcttcgg ttttggtttt tctcataatg
     1621 gcgttttcgt ttcgggtcgg tttgacgagg gcggattata aaaaagactt ttatccgtgc
     1681 aatcgtgtat gtataatgaa acccataaaa ttgagactgc atctcaactt tgaaaacagg
     1741 tataacctgc tttgcaaccc gttcgaacat tcgggtatca aagcaggcgg tttttatatc
     1801 ctgaaataag aatgcctgtt gattggcaaa accctttaca tcaaaccaga cagataggat
     1861 aaataatggc tttttatgct tctaaggcga tgcgtgcggc cgcgttggct gccgccgttg
     1921 cattggcact gtcgtcttgc ggtaaaggca gagacgcggc gcagggcggg cagcctgcgg
     1981 gtcgggaagc ccccgcgccc gtcgtcggcg tcgtaaccgt ccatccgcaa accgtcgcat
     2041 tgaccgtcga gttgccgggg cgtttggaat cgctgcgtac cgccgatgtc cgcgcccaag
     2101 tcggcggcat catccaaaaa cgcctgttcc aagaaggcag ttatgtccgc gccgggcagc
     2161 cgctgtatca gatcgacagt tccacttatg aagcaggtct ggaaagcgcg cgcgcgcaac
     2221 tggcaacggc acaggcaacg cttgccaaag cggatgcgga tttggcgcgt tacaaaccgc
     2281 tggtttccgc cgatgccatc agtaaacaag agtacgatgc tgcggtaacg gcgaaacgtt
     2341 ctgccgaggc gggcgttaaa gcggcgcagg cggcgatcaa atccgccggc atcaatctga
     2401 accgttcgcg cattaccgcg ccgatttcgg gctttatcgg tcagtccaaa gtttccgaag
     2461 gtacgctgtt gaatgcgggc gatacaactg ttttagccac catccgccaa accaatccga
     2521 tgtatgtgaa cgttacccag tctgcatccg aagtgatgaa actgcgccgg cagatagccg
     2581 aaggcaagct gctggcggcg gatggtgcga ttgcggtcgg catcaaattt gacgacggta
     2641 cggtttatcc tgaaaaaggc cgcctgctgt ttgctgatcc gaccgttgac gaatcgaccg
     2701 gtcagattac cttgcgcgcc gccgtatcga acgatcagaa tatcctgatg cccggcctgt
     2761 atgtgcgtgt gctgatggat caggtggcgg cggataatgc attcatcgtt ccacagcagg
     2821 cggtaacgcg cggtgcgaaa gataccgtga tgattgtgaa tgcccagggc ggtatggaac
     2881 cccgcgaggt aacggtcgcg caacagcagg gtacgaattg gattgttacg tcgggtctga
     2941 aggacgggga caaggtggtt gtggaaggca tcagtatcgc cggtatgacg ggggcgaaaa
     3001 aggtaacgcc taaagaatgg gcgccgtctg aaaatcaagc tgccgcccct caagccggcg
     3061 ttcagacggc atctgaagcc aaacctgctt ctgaagcgaa ataaggaagg catcgatggc
     3121 taaattcttt atcgaccgcc ccattttcgc gtgggttatt tcgattttca ttattgcggc
     3181 aggtattttc ggcattaaaa gcctgccggt ttcgcaatat ccgtccgttg ccgccccgac
     3241 cattactttg cacgccattt atccgggtgc gtccgcacaa gtgatggaag gcagcgtact
     3301 ttccgtgatc gaacggaata tgaacggcgt ggaaggtttg gactatatgt ccacttccgc
     3361 cgattcgagc ggcagcggca gcgtgagcct gacctttacg cccgataccg acgagaatct
     3421 ggcgcaggtg gaagtgcaga acaagctttc cgaagtattg agcacgctgc cggcaaccgt
     3481 ccagcaatac ggcgtaaccg tatccaaggc gcgttccaat ttcctgatga ttgtgatgct
     3541 ttcgtcggat gtgcagtcaa ccgaagagat gaacgactac gcgcagcgca acgtcgttcc
     3601 cgaattgcag cgtatcgaag gcgtggggca ggtccgcctg ttcggcgcgc aacgcgcgat
     3661 gcggatttgg gtcgatccta agaaactgca aaactacaat ttgtcgtttg ccgatgtcgg
     3721 cagcgcgttg tcggcgcaga atatccaaat ttccgcaggt tccatcggtt cgcttcccgc
     3781 tgtgcgcgga cagacggtta cggctaccgt aacggcgcaa gggcagttgg gtacggcaga
     3841 agaattcggc aacgtcatcc tccgcgccaa taccgacggt tctaatattt acctgaagga
     3901 tgtggcgaaa gtcggattgg gtatggaaga ctattcttcc tcaacccgcc tgaacggggt
     3961 aaataccacc ggtatggcgg taatgttgtc caacagcggc aatgcgatgg cgacggcaaa
     4021 ggcggtaaaa gaacgcttgg cggtgctgga aaaatacttt ccgcagggta tgagttggaa
     4081 aaccccttac gatacttcca aattcgttga aatttcgatt gaaaaagtga ttcacacttt
     4141 aatcgaagcg atggtgctgg tgtttgtcgt aatgtacctc ttcctgcaaa acatccgtta
     4201 tacgctgatt ccgaccatcg tcgtaccgat ttccctgttg ggcggtttcg ccttcatctc
     4261 ttatatgggg atgtcgatta acgtattgac gatgtttgcg atgacattgg tcatcggcat
     4321 cgtggtcgat gacgcgattg tggtggttga aaacgtcgag cgcattatgg cgggtgaagg
     4381 cctgccgccc aaagaagcga ccaaaaaagc gatgggtcag atttcgggcg cggtcatcgg
     4441 tattaccgcc gttctgattt ccgtgttcgt gcctctggcg atgttcagcg gggcggccgg
     4501 caatatttac aaacagtttg ccctgacgat ggcgtcatca atcgcattct ccgccttcct
     4561 tgcgctgacc cttacccctg ctttgtgtgc cacaatgttg aagacaatcc cgaaagggca
     4621 tcacgaagag aaaaaaggtt tcttcggctg gttcaacaag aaattcgaca gttggacgca
     4681 cggctacgaa ggccgggttg ccaaagtgct gcgtaagact ttccgcatga tggttgtcta
     4741 tatcggcttg gcggttgtgg gcgtgttcct gtttatgcgc ctgccgactt cgttcctgcc
     4801 gaccgaagac caaggcttcg ttatggtcag cgtgcaactg cctgcgggtg cgaccaaaga
     4861 gcgcaccgat gcgacgctag cacaagttac ccaactggcg aaaagcattc ccgaaattga
     4921 aaacatcatt accgtttccg gcttcagctt ttcgggcagc ggtcagaata tggcgatggg
     4981 ttttgccata ttgaaagatt ggaacgaacg taccgcctcc ggcagcgatg ccgttgcggt
     5041 tgccggcaag ctgacgggta tgatgatggg gacgcttaaa gacggtttcg gcatcgccgt
     5101 cgtcccgcct ccgattctgg agttgggcaa cggttcgggc ctgagcatca acctgcaaga
     5161 ccgcaacaat accggccata ccgcattgct ggcgaagcgc aacgagctga ttcagaaaat
     5221 gcgtgccagc ggtttgtttg accccagcac cgtccgtgcc ggcggtttgg aagactcgcc
     5281 gcagttgaaa atcgacatca accgtgccgc agcggcggcg caaggcattt cgtttgccga
     5341 catccgcacc gcattggcaa gcgcgctgag ttcgtcttat gtcagcgact tcccgaacca
     5401 aggccgtctg caacgcgtga tggtgcaggc cgacgaggat gcccgtatgc agcctgccga
     5461 tattttgaac ctgaccgtgc cgaacaaatc cggcgtcgcc gtaccgcttt ccaccattgc
     5521 tactgtttct tgggaaaacg gtacggaaca gagcgtacgc ttcaacggtt atccctcgat
     5581 gaagctgtcc gcttcgcctg caaccggcgt ttccaccggg caggctatgg cggcggttca
     5641 aaaaatggtt gacgaattgg gcggcggtta cagcttcgaa tggggcggac agtcgcgcga
     5701 agaggcaaaa ggcggctcgc aaaccctgat tttgtacggt ttggcggttg cagccgtatt
     5761 tttggtactt gccgcgcttt atgaaagctg gtcgattccg ctggcggtca tccttgtgat
     5821 tccgttgggt ttgatcggtg cggctgcggg cgtaaccggg cgcaacctgt ttgaaggact
     5881 gttgggcagc gttccctcgt ttgccaacga catctacttc caagtcggtt tcgttactgt
     5941 gatgggtttg agtgcgaaaa acgcgattct gattatcgaa tttgccaaag accttcaagc
     6001 gcaagggaaa agcgcggtcg aagccgcttt ggaggcagcc cgcctgcgtt tccgtccgat
     6061 tatcatgacc tcgtttgcct ttattttggg cgtggttccc ctgtatattg ccgctggtgc
     6121 gagttctgcc agccagcgcg ccatcggcac gaccgtattc tgggggatgc tcatcggcac
     6181 gctcttgtcc gtgttccttg ttccgctttt ttatgtagtg gtgcgcaaat tcttcaaaga
     6241 aactgcacac gagcatgaaa tggcggtcag acatgcatca aaagcgggta taaccggttc
     6301 ggacgataaa caatattaag caaaataccg tctgagaacc gccggttttc agacggcatc
     6361 aggacttttt tatgaatact acattgaaaa ctaccttgac ctctgttgca gcagcctttg
     6421 cattgtctgc ctgcaccatg attcctcaat acgagcagcc caaagtcgaa gttgcggaaa
     6481 ccttccaaaa cgacacatcg gtttcttccg tccgcgcggt tgatttgggt tggcatgact
     6541 attttgccga cccgcgcctg caaaagctga tcgacatcgc actcgagcgc aataccagtt
     6601 tgcgtacagc cgtattgaac agcgaaatct accgcaaaca atacatgatc gagcgcaaca
     6661 acctcctgcc cacgcttgcc gccaatgcga acggctcgcg ccaaggcagc ttgagcggcg
     6721 gcaatgtcag cagcagctac aatgtcggac tgggtgcggc atcttacgaa ctcgatctgt
     6781 tcgggcgcgt gcgcagcagc agcgaagcag cactgcaagg ctattttgcc agcgttgcca
     6841 accgcgatgc ggcacatttg agcctgattg ccaccgttgc caaagcctat ttcaacgagc
     6901 gttatgccga agaagcgatg tctttggcgc agcgtgtctt gaaaacgcgc gaggaaacct
     6961 acaagctgtc cgaattgcgg tacaaggcag gcgtgatttc cgccgtcgcc ctgcgccagc
     7021 aggaagcctt gattgaatct gccaaagccg attatgccca tgccgcgcgc agccgcgaac
     7081 aggcgcgcaa tgccttggca accttgatta accgtccgat acccgaagac ctgcccgccg
     7141 gtttgccgtt ggacaagcaa ttttttgttg aaaaactgcc tgccggtttg agttccgaag
     7201 tattgctcga ccgtcccgac atccgcgccg ccgaacacgc gctcaaacag gcaaacgcca
     7261 atatcggtgc ggcgcgcgcc gcctttttcc cgtccatccg cctgaccgga agcgtcggta
     7321 cgggttctgt cgaattgggc gggctgttca aaagcggcac gggcgtttgg gcgttcgcgc
     7381 cgtctattac cctgccgatt tttacttggg gaacgaacaa ggcgaacctt gatgtggcaa
     7441 aactgcgcca acaggcacaa attgttgcct atgaatccgc cgtccaatcc gcctttcaag
     7501 acgtggcaaa cgcattggcg gcgcgcgagc agctggataa agcctatgac gctttaagca
     7561 aacaaagccg cgcctctaaa gaagcgttgc gcttggtcgg actgcgttac aaacacggcg
     7621 tatccggcgc gctcgatttg ctcgatgcgg aacgcagcag ctattcggcg gaaggtgcgg
     7681 ctttgtcggc acaactgacc cgcgccgaaa accttgccga tttgtacaag gcgctcggcg
     7741 gcggactgaa acgggatacc caaaccggca aataaccgcc cgggcaaaca aatgccgtct
     7801 gaaacgataa ggtttcagac ggcatttctt tctttatagt ggattaacaa aaaccagtac
     7861 ggcgttgccc cgccttagct caaagagaac gattccctaa ggcgcccaag caccgggcga
     7921 accggttccg tactatttgt actgtctgcg gcccgccgcc ttgtcctgat ttttgttaat
     7981 ccactatatg tttgacggtt gcggtcaggc tgcgcggcgt gccgtaaacg tgaatgtcgg
     8041 gcatcgtgcg gcagcgtttg ttaaagacgt tttcaaagtc caaaccgatg cgggtggatt
     8101 tgccgatttt gtaatgcgcg gtcaagtcca gtgtagcgta tggggcgttg ggtcaatccg
     8161 gctgcggcga cagggaagaa accgccatac ccgtcggcat cccgaagccg aatacggcaa
     8221 cggcaagcgc aatcagcagc gtataaagcc ccgcctgata gcctttcagc ttcaggacgg
     8281 tcagcgcggc aaagaaaaag acgatgggta agagtgcggc ggcggcagtc agatacaggc
     8341 tgccgccgat tgccgtgtag ttttgaaccc aagtttccat aattgaacat ctccgaaaat
     8401 atttttctaa tcgtcggcaa tagtggtcaa accaattaaa gcaacgttgc attactttac
     8461 gaaactttaa tattcaggtc aatatatttt tgggcggttc ggcagatttg aatcggagct
     8521 ttcatttaaa tcccgttaaa acaaatattt gcatgaacaa aaattgtagt ttggtgtagt
     8581 tttttcctgt gtttcggggg cggtgcgagg taagtgccgt tgacggcggg aacggcgtgt
     8641 cattagaatc cgccctgatt ggccagtcca atttgatgtt tgatgtattt cggaagggtt
     8701 ctgcagggaa tctttataaa ggaggagtga tgaaactggt aaggtctcag aaaatcagcg
     8761 atcaggtatt gtcggtattg gaaggccgca tcgtggaagg ggtttacgcg gaagggggca
     8821 agattccgcc cgaacgcgtt ttggcggaag agttcggcgt ttcgcgtccg tcggtccggt
     8881 cggcattgaa tattttggtt gcccgtcaga ttttggaagc gaggcagggc gatggttatt
     8941 atgtttcggt caagccgcag caggattttt tgcaaagttg gcaggagctt ttgggcaaac
     9001 attccaattg ggaacaggat gtttttgatt tcagctgcca catcgagggc tgtatggcgg
     9061 cattggcggc agaacgccgg acggatgccg atttgaagcg gattcggttt tggcttgaaa
     9121 agtttgagga agcgtgcgga agcggcaatc tggaacatca gggcgaagcc gatgtcagct
     9181 tccatcagac gattgcggat gcggcgcaca atttgttgtt cagccatttg tcgggcggtt
     9241 tgttgaaaat gctgtaccgg cagacgcgca gcagccttat ttatctgaat caggaagaag
     9301 atccgcgtcc gaaactgatg gcgcagcacc gcgtgctgta tgaggcgata tcgaaccgcc
     9361 ggccgggcga ggcttcggag gcggcaaagg ctcatttgaa ttatgtggcg agcagcatat
     9421 tgaaagacag ggaatatcag agccgtaacc gccacgcgga tactttggcg caaaacgatt
     9481 tgaagcgcgt gcaggattgg gaggtatgaa taccgttcag acggtctggc atattcggat
     9541 gtatcggtat agtgaactaa atttaaacca gtacggcgtt gcctcgcctt gccgtactat
     9601 ttgtactgtc tgcggctcgc cgccttgtcc tgatttaaat ttaattcact atagtattac
     9661 ccgattcgaa cgggcggata atgaaatgcc gtctgaacag catttgcggt tcggacggca
     9721 ttttgtttgc ggggcggagg cgtttgctta gaagccttgc gccagcatcg catcggcaac
     9781 tttgacgaag ccggcgatgt tcgcaccgtt gacgtagttc actttgtcgc cgactttgcc
     9841 gtatttcagg caggactcgt ggatgctttg catgatgccg aacagtcggc tgtccacttc
     9901 ttcgcgcgcc caagacaggc ggatggcgtt ttggctcatt tccaagcccg aagttgccac
     9961 gccgccggcg ttggaggctt tgcccggagc gtagaggatg ccggctttga taaattgctc
    10021 gaccgcgccc aaagtcgacg gcatattcgc gccttcggca acgacgtagc agccgtttgc
    10081 caacagggtt ttggcggctt cttcgtccaa ttcgttctgg gtcgcgcagg gcagggcgat
    10141 ttcggcggca acgccccacg gtttttggtt ttcaaagtat tgcaaacctt gctctttggc
    10201 gtaggtggca acgcgttcgc ggcggacttc tttcaattcg atcaaggcgg cgagttgcgc
    10261 ttcggtcata ccgatgtcgg ggaagaggac gaagccgtcg gagtcggaga cggtcagcac
    10321 tttcgcaccc agttggatgg ctttttcagc ggcgtattgc gccacgttgc cggaaccgga
    10381 aatcagaacg cgtttgcctt caaaactatc gttgcgggtt tgcagcatcg cttgggcgaa
    10441 atagacgcag ccgtagccgg tcgcctcggg gcggatgagg ctgccacccc attccaaacc
    10501 tttgccggtc aggacggaag tgaattcatt gcggattttt ttgtattgtc cgaacaggta
    10561 gccgatttcg cgtccgccta cgccgatgtc gccggccgga acatcggtgt ccgcgccgat
    10621 gtggcggtag agttcggtca taaaggcttg gcagaagcgc attacttcgg catcggattt
    10681 gcctttgggg tcgaagtcgg aaccgccttt gccgccgccc ataggcaggg tggtcaaggc
    10741 gtttttgaac acttgttcaa aagcgaggaa tttcaacacg cccaaatcga cggtcgggtg
    10801 gaagcgcagg ccgcctttgt aagggccgat ggcggaactc atttgcacgc ggtagccgcg
    10861 gttgacttgg acttgtcctt tgtcgtcctg ccaggttacg cggaacatca cgacgcgttc
    10921 gggttcgacg atgcgttcca gcaggctttg ctgggtgtat ttcggatttt ttgccaaaaa
    10981 cggatcaagg ctcatgaaga cttcttcaac cgcctgatgg aacggctcct gattggggtt
    11041 gcgttgtttg aggttggcaa acagggtgtt caggtcagtc attcggatac tccttttggt
    11101 tgatattgta ctaatgggaa aacaaatttg tagttggcaa tataaaccta tttttttctg
    11161 atgccaagcc taattttgca caaattgttg acattatttt tcccatattt tcgatatatg
    11221 atgtttattg ccgataatca accaataatc ataaattaat ttctcaaata aaaaatatta
    11281 ttggaatttt attctaagta gttaaactag attttttgat tccaatcaag ataattttgt
    11341 tttcacgcct gcttttgctg tgaaaaaggg ctgaaattgc caacaatcct gcctgccttc
    11401 atccgcgttt tgagcgtccg gcggggcggc ctgcgcgttt gtccggactc gggtgttggc
    11461 cggaaatgtt acaatatgtg ccgtttaatt tgatgaaaga tgatgatgaa ggcctatttg
    11521 gacttgatgc gccacgtcct cgacaacggt acggacaaat cagaccgcac cggtacgggt
    11581 acgcgctccg tattcggtta tcagatgcgt tttgacttgg gcaaaggttt tccgctgctg
    11641 acgaccaaaa agctgcattt gcgctcgata atccacgagc tgctttggtt cctcaaaggc
    11701 gataccaata tcaaatacct gaaggataac aatgtttcca tttgggacga gtgggcggac
    11761 gaaaacggcg acttgggtcc ggtttacggc taccaatggc gcagctggcc cgcgcccgac
    11821 ggcaggcata tcgaccaaat cgccaatgtg gtggaacaaa ttaagaaaaa ccccgactcg
    11881 cgccgcctga ttgtgtcggc gtggaatccg gctttggtcg atgagatggc cttgccgccc
    11941 tgccacgcgc tgtttcagtt ttacgttgcc gacggcaaac tgtcctgcca gctttaccag
    12001 cgtagtgcag atattttcct tggtgtgccg tttaatattg ccagctacgc gctgttgacg
    12061 atgatgatgg cgcaagtgtg cgggctggag gcgggcgagt ttgtccatac gtttggcgac
    12121 gcgcatttgt accgtaacca tttcgaacag gccgcattgc agttggaacg cgaaccgcgc
    12181 gccttgcctg tgatgaaaat caatcctgaa gtcaaggatt tgtttgcctt caagtttgaa
    12241 gatttcgagt tggaaggcta cgatccgcat ccgcacatca aggcggtagt gtcggtttag
    12301 gaggcggtat gggcaggcat ttcgggcgca gacgttttct gacggctgcc gccgttgctg
    12361 tggccggtgc ggcggtttct tttttgccga atccttttgc cgccggcggc gaaaaacgca
    12421 acatggataa aaaacgcgat gaaaatgtgt ttttctggaa aggtgtcgcg ctgggttccg
    12481 gcgcggagct gcgcctgttc ggcgtggacg acagacaggc ggcggatttg gtcaataagg
    12541 ttttggcgga agtggcgcgt ttggaaaaaa tgttcagcct ttaccgtgaa gacagcctga
    12601 tcagccgtct gaaccgcgac ggttatctga cttcgcctcc ggcggatttt ttggaactgt
    12661 tgagcctggc cgcgatattc acgcgctgac ggaaggcgcg ttcgatccga cggttcaggc
    12721 gttgtggaaa ctgtatgccg attattttgc cggccgtccc gatgcggaaa cgccgccgga
    12781 gaatagcgtg aaggaaaccc tgaaacgggt cggcttcggc aaagtctcgt ttgacgaccg
    12841 tgaaatccgt tttgccgaaa agggtatggg tttgtccttg aacggcatcg cgcaaggcta
    12901 tatcacggac aaggttgttg cgctgcttaa agcaaacgga gtgcctgccg cgctggtcga
    12961 tatgggcgaa atcagggggt ttgatacaaa cggcaggcgg atgtggaatg tcggcatccg
    13021 caatccggac gacgaagaag gcgtgttggc aaatatcacg atgaaagaca aggcgtttgc
    13081 cacttcgggc ggctatggga cggtgatgga caaagcgggc aggcttaccc atttgttcga
    13141 tccgcgaacc ggcgtgagta cgccgcgtta taaaagcatg agcgtaatgg cggatgatgc
    13201 ggcggttgcc gatgccttgt cgacggcgtt ttccgtgatg gatttgccgc tgatacggtc
    13261 ggttgccgaa agcaggcggc ttaaagttag gctggccatg ccggacaata tagtggatta
    13321 acaaaaaccg gtacggcgtt gcctcgcctt gccgtactat ctgtactgtc tgcgacttcg
    13381 ccgccttgtc ctgatttaaa tttaatccac tatactttga acgaaatagg ttgaatctga
    13441 agcaaaatgc cgtctgaaag gctttcagac ggcattttgc cgtcgggcgg tatgtttaaa
    13501 ggatgcctaa agaattgacg aaaacaataa taatggcaat cggggcaagg tagcgcaagg
    13561 tattcagcca cagcagcagc actgcccgtg gtacggtgct gcccgcgccg gcatctttta
    13621 acacagactg cttgtcctga atccaggcgg taaagatgga aacactcaaa gcaccaatcg
    13681 gcataatgac ggcggaaata acatagtccc acaaatcaaa aatggttttg ccgaaaacct
    13741 taaactcacc ccatgcgccg aaagacagcg cggacgggat gccgacaatg aaaatagccg
    13801 tgccgataag ccaagtatgt tttttgcgtt tgcgctcgtc ttggcggatg gttgaggcaa
    13861 tgaccgtttc caacatcgaa aatgccgaag tcagcgtggc gaaaacgacc agaagcatga
    13921 atactgcaaa taaaaccgta ccgaacggca ttttcatgaa cactgcgggc aatacgataa
    13981 aaatcaatcc cggcccctgg ttcggttcaa aaccgaaggc gaataccgcc gggaaaatca
    14041 ccaggccggc aagcagggaa accaagaggt tcatccacat aatcgtatgg ccggaacgga
    14101 acatatcctg atcttttccc aaatacgaag cataggtaat catggcggaa acgccgatgc
    14161 tcagggcaaa aaacgcctgg cccaaagccg taatcatcgt atcggcttta aagtacgacc
    14221 aattgggttt gagcaggaaa gacacgccct ccattgcatc cggcagcgtc agcgaacgga
    14281 cagccagcgc aataaagagg ataaacagcc ccggcatcag ataacggttt gccttttcaa
    14341 tgccgtctga aatgccgcct ttgaccaccc aaaccgtaat cagcataaac agtgcctgat
    14401 aggacagcga acctgccgga ttggaaatcg ttgtgccgaa cagggcttca aagtccgctc
    14461 ccgcatggat tgcccctgta aaactgtgga cgacataatt caatacccat ccgccgacca
    14521 cgctgtaaaa cgacagcaaa ataaagcagg cggcaacgcc catgcgcccg acccaaagcc
    14581 attgcgtacc cggacgcaga accctgaagg aatcgacggc atttttaccg cccgtgcgcc
    14641 cgatataaaa ttcggcaagc tggacgggca gggcgaccaa gacggtaaat atcaaaaaca
    14701 gcaggaaaaa caccgcgccg ccgttggtgc ccgccgtata aggaaatttc caaatcgcgc
    14761 ccaaaccgat ggccgaacct gccgcagcaa gtacgaaacc gattttggac gaccaagaag
    14821 tatggttact cataaaattt attaagaaat gtgaaggtag tgcattgtaa caaagcactt
    14881 gacgcggcgg cacaagccgg ggaagacgct gcaaaacttt gccaataggc tgcgcgatat
    14941 cgggggatat tgggattttc ttattttgaa ggatgaattc ggggtgtttc tttccaatga
    15001 acgaatataa tttggcggtt tgttgcatca aaggctttca gacggcatag gccgtctgaa
    15061 agcggcttat attgcatcca ataatttgtc ggcggtatgg ttgatggtga aacgggcggc
    15121 gacgcattga tgtcctgccg ttgccagtgc gttttttgct tcggggttta agacgaggcg
    15181 ttcgatttcg tccgccaaag attcaggcgt aagcctgtcc agcagaatcc ccgattgatg
    15241 atgttcgata atttcctttt gcgcacccaa agtattggaa atcaccgccg ttcggcagta
    15301 catcgcctcg cataatgaca aaccgaatgc ctccgggacg aggctgggca aaaccacgac
    15361 atcgctttgg cggtaaaacg aagcaagttt ttcggtaaac ccttcaaaag aaacaaatgg
    15421 ttctgctccc gaagcagata cgccccgctt caggcggcac atataatccg gatgtccgtg
    15481 ccctgccaat ttgagcctga tttgaggata tttccgatgc agtatcacac aggcttcaat
    15541 caggttttcc aatccttttt ccggactgat cctgccggcg taggcgacgg taaaaaaacg
    15601 gctgtcgggt ttttcttgag acggggggaa ccggccggta tcgataccgt tatgaacaat
    15661 ccggtatttt tctttaaagg gattgtcggc ggtttgcaca tcgtaaacca gacgggaaac
    15721 gcaaataaag cggtcggtgt ttttctgtat caggcggtga taaaaatcgg ttttgttggc
    15781 gacgacatta tgtttgacaa atatcaggcg caccccggtc agttttttca aaagtacgga
    15841 taaggcggca attttgccgg tgtgtatcat caggtgggaa atgtggtttt cccggataaa
    15901 gcgggtaagc gcgtagagcg aaaacagccc gttgaacgtg tgaaggcgcg ttgtgaaaac
    15961 ggaagaaact tcggaaaagc gtctgcgcat caattcatca tttttattga cggcggtaaa
    16021 catggtgcag ccccgaagtc ccaatgcttt tgaaacatca tagacatatt gttcgccccc
    16081 accccaaatc ttggccgata cgatatttaa tatcctgaat tctttttcca tttaaaattt
    16141 tccgccggat ttgtacagat aatatagttt gacatattta atcatcgtgt aatagctgtg
    16201 gttgacggac ataatccaac ccatttttcc atcaagaaac cctttgttca ggatataaat
    16261 tttgaaaaaa ccccaaatcg gacggaggat aatgtcccta acgaaactca cgggctttcc
    16321 ctgctcccgg tatttctcgg ccgagatgga agtatatttg ttgaacttgt tgaaatattg
    16381 ttcccagttg tcgtacgtgt aatggtacat aaaatgtttc agacggcgtt cggggtaggg
    16441 ggtctgtacg gtttcgtgta ctttgccttg caccgaaccg ccttttttcg gcatcagacg
    16501 gcatacgctg tcgggacgca tcgcgccgtg tgtggcggga tggttgggga aaaggttgcg
    16561 gcgttcgaca aaataggcgg cataatcgcc ggttcggaca atttttgaga tttcatcaga
    16621 tagttccggc gtgcagcgtt cgtctgcatc aatcaggaaa acccattctc cgcctgcctg
    16681 ttcgatggca aatgtttttt gcgctccgaa atccccgttc aaatgccgtc tgaagacttt
    16741 tgcacccaaa ccctcggcaa tttcggcagt gttgtcggcg ctatggtcgt cgataacgat
    16801 aacttcttta tcgaaacggc aactttcaat acattcccga atgtggtttg cttcgttttt
    16861 ggcaacaatc aatacgctga cttttttcat tatcaaggtt tctcaaagca ttttggtttc
    16921 acagggcggc aaaaaagccc aaacatacgt ccgggcttgg gaattggtgg gtccggtggg
    16981 gttcgaaccc acgaccaagg gattatgagt cccctgctct aacccctgag ctacagaccc
    17041 gtcaagagta ggcgcgtatt gtatagcata gcggtgggat tgacaaactt atttcatccg
    17101 atgattaaga cgcagttgct tcccccgaag gcaaacgacg agctggcggc aatgcgtttt
    17161 tcggtttccc agccgtcgcc ggtcaggtta atggcgggca ggtcggggtc gttctgcccg
    17221 tcccaaagcc ggggcggaag tttcccttcg ggattgcttt gccggtcggc aatgccccac
    17281 gcgaacgcgg cttcgattgc gcccgccgcg cccagcgtgt gtccggtttg cggcttggcg
    17341 gacgtgcagg gcgtattgtt gccgaaaacc gctgcaacgg cgcggctttc catactgtcg
    17401 ttgtggtgcg tcccggtgcc gtgcagatta atccggccga tgtcttcggg cgcaaggccg
    17461 gcgtgctgca atgccgtctg aaaggcgagg attgcgcctt tgcgtcgggg cggggcgtgg
    17521 aaatatggta ggcatcgctg cttgcgccgt aacccagaag ctgcataccg ccggaaaaat
    17581 ccgcatcgcg cgtcatgatg aatacggctg cgccttcgcc gatattgatg ccgtcgctgt
    17641 tggcgccgta gccgacgagt tcggcgtaaa ttttcgcacc gcgccgtttg gcgtgttcca
    17701 attcttccaa tacgaaaatc cccgcgcctt ttctccgatg accagcccgt cgcggttcgc
    17761 gtcgtatgga cgcggggtct cttccggttc gccgttgcgg cggctggcgg catagagcga
    17821 gtcgaaaaca tacacttcgg acgggcaaaa ttcttcgccg ccgcccgcca gcatcatatc
    17881 ggtcagaccg tatttgatgg cctcgtaagc ataacctatg ccttggctgc ccgaagaaca
    17941 cgcgctcgat gtcgggatga tgcgtccttg ttaatccgaa aaagatgccg atattggcgt
    18001 cggcggtgtg cggcatcata cgcacatagg tattggcgcc gaagttgcgc gacgtgccgg
    18061 tcagcaacaa ttctcccaca tctccgatgt ctttggtgcc gccgccggaa gagccgcagg
    18121 caacgcccat ccgtccgtcg gtaatgcttt cgtctccgag caaaccggca tccgccagcg
    18181 cctgctccgc cgcatcgacg cacaggtacg acacgcgccc catacttctg agccgcttgc
    18241 gcgtccaatg tttcggcggc gcgtaatcct caatcggcgc acccagttgc gcttccaatt
    18301 cggggaaacg ttcgcgccaa tccatatatt tgacggcgtt tttttcggct ttgaatgctg
    18361 cctgtatgct ttgccaatcc cggccgaagg cggtaatgcc gcctatgccc gttactgcga
    18421 cccttctggt attcaacaca aacctccgtt caccgcaatc acctggcgcg tgatatacgc
    18481 cgctttttca tccatcagga agtgcactgc gtgcgccact tcttccggca gtcccatacg
    18541 cgcggcgggg acggctttca aaatttctcc gacgggtacg ttttcatcga caatctccgt
    18601 atcgatcaaa cccggcgcca cgcagttgac ggtgattttg cgtttcgccg gttcgaccgc
    18661 caaggctttt gccgcgccga taatgcccgc ttttgacgcg ctgtaattga cctgcccccg
    18721 gttgcccgtc aggccggaca ccgatgccat acacacaatc cgtccggctt tgcggcggcg
    18781 tatcatcggc ataaccagcg gatgcagtac attgtaaaaa ccgtccaaat tagtccgcgg
    18841 caccacatcc caatcgtcgt ctgaaaacgc ggggaaggca ttgtcgcgcg tcaggccggc
    18901 gttcaacacc acgccgtaat acgcgccgtt tgcttcgatg tcggcggtca gaatctcgcg
    18961 gcaggcttcg cggtcggaca cgtcaaactg caacacgcgc gcatttctgc ccaaagcgcg
    19021 gatttcttcc gccacggctt cggcttcgtc gcggcggctg cggcagtgga cggcgatatc
    19081 aaagccgtcg tccgccaaaa ccgagtgcgg cggctttgcc tatgcctctg tttgagccgg
    19141 taatcaggac ggtttcagtc atggttgttc tccgtatgtg cgtgcctgtt cagacggcat
    19201 taagttgttt cgacagggtg ttcggggctg tacacgttga gtgaggcgcg cgccaaaatg
    19261 ccgtctgaaa ggagtgtttc agacaaggtt tccggcgcgt ccgtccaacg cagttcgcag
    19321 tcggacacgc ccataccccg gcatcttgga tagacatatg cgccgttgcc ggcaaatgtg
    19381 ttccgacagg gacggattgg gtaaaaattt caagtttgcg cgtgccgggc aggaaaaccg
    19441 agccggaccg gttgcgcctt ttttcgggct tggaggccgg catatgcgcc gacagcctac
    19501 gccatcagtt cgataaatgc cgtatagggc agcatgccgc cggccagcag gatgtggtcg
    19561 ggttttacct gcgcccctgc ttcgacaaaa tcatcgccgt atcgggtaat gcggtctatc
    19621 agaaccatac gcccgctgtg cggcagcagg gaggcggtgt cggtaatcgg gcattataat
    19681 gataaatgta aattcaggct aattcgggcg gtttgccgag gggcggcgtt gtcggaaaaa
    19741 atggcgtgga tgcgtttgaa tttcggcatt gtcaggggga tgggctttgg ttagaatgcg
    19801 gcacagtgta aatttttgaa agaaactatg ccgccgatta tttgccggtt ccacttcgac
    19861 atcgccgact ggcgggtttc gggcggcaaa atgcgcgata tggcccagta gaggcagtgg
    19921 gcggaatgtc cggattttgc cgacggtttg cccgatgtca ggcgggaatt gccgttcctg
    19981 cccgctatgc ggcgccgccg tttgagtaag gcggcgcgtt tggtatgcga cgcggcttgg
    20041 gacattgcct ccgcccatcc cggcagtccg gtagtttatg cctcgcacga cggggaaatg
    20101 gcgcgtagtt tcgatttgtg gttggaactg ttgaaatcgc ataccgtgtc gccgacttcg
    20161 ttcggtttgt cggtgcacaa tgcgacggcg gggcaatggt cgatattgag gcgggatatg
    20221 agcgagcaga cggcattggc cgtgtgtgcc gacggtgtgg aaacggcatt ggcggaagcg
    20281 gcgtccctgt tggaagaggg atgcgggtcg gttttggttt tggcggcgga cgatccgctg
    20341 ccggaaggat atgccgtatc ggcaacgcgc gcgccgtttg cttatgcttt ggcgatggtt
    20401 ctgaccaaag ggacgcgtta cagcctgact ttgtctgctt cggacgatat gccgtctgaa
    20461 gccggtatgc tgcccgaggc atattggagc ggtttggaat gggtgcgttt tctgctgaac
    20521 ggcagtcggg aatgccgccg ggtatatcgc aatcgtgaat ggttgtggca gcccgcaagc
    20581 tgcaggctga agatttccgc aatgtccgca ccgtcaacga cgtggtacag gcggtattga
    20641 agattcaggc cggctaatcg ggtatttcta attatttttc agacggcatg tttttatcgg
    20701 atgccgtctg aaaacgtttg tgcggattca aaatgaaacc cggttttttc ggacatattt
    20761 ttttgaccgt attaagcatt gcctatcccg ctttgtg
//
LOCUS       DAXDSD010000029        20755 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXDSD010000029 DAXDSD010000000
VERSION     DAXDSD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20755
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.029"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(126..326)
                     /locus_tag="ACFB2Q_000836"
     CDS             complement(126..326)
                     /locus_tag="ACFB2Q_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917889.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
     gene            complement(340..894)
                     /locus_tag="ACFB2Q_000837"
     CDS             complement(340..894)
                     /locus_tag="ACFB2Q_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917890.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            complement(979..1479)
                     /locus_tag="ACFB2Q_000838"
     CDS             complement(979..1479)
                     /locus_tag="ACFB2Q_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917891.1"
                     /translation="MDAMPQTAVQNENLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANKRACRFAEKHRLFFTPAQAELSLNKHKNRRLLTIETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            1576..1785
                     /locus_tag="ACFB2Q_000839"
     CDS             1576..1785
                     /locus_tag="ACFB2Q_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917892.1"
                     /translation="MGWISSKRKKAPHVCVVPIAVSGCKRFALHDEMPSEDKNIGEIL
                     NQNAAAPQALYRNFFVFFSYPVENI"
     gene            1884..3077
                     /locus_tag="ACFB2Q_000840"
     CDS             1884..3077
                     /locus_tag="ACFB2Q_000840"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFC0917893.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(3184..4428)
                     /gene="clpX"
                     /locus_tag="ACFB2Q_000841"
     CDS             complement(3184..4428)
                     /gene="clpX"
                     /locus_tag="ACFB2Q_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC0917894.1"
                     /translation="MSNENRTCSFCGKSKSHAKHLIEGKNAYICDECVANCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            4611..4982
                     /gene="rbfA"
                     /locus_tag="ACFB2Q_000842"
     CDS             4611..4982
                     /gene="rbfA"
                     /locus_tag="ACFB2Q_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC0917895.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     VTRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            4993..5517
                     /locus_tag="ACFB2Q_000843"
     CDS             4993..5517
                     /locus_tag="ACFB2Q_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917896.1"
                     /translation="MNNLIPEHLAAYAHSDNLQIEGGHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSQNQKQNRTLTDL
                     DEYTYRVPIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISIDLID
                     EKHSVRELLNEELS"
     gene            5575..6495
                     /gene="truB"
                     /locus_tag="ACFB2Q_000844"
     CDS             5575..6495
                     /gene="truB"
                     /locus_tag="ACFB2Q_000844"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC0917897.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLDETERDNLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRMKALRLMNTAASSA"
     gene            6713..7225
                     /locus_tag="ACFB2Q_000845"
     CDS             6713..7225
                     /locus_tag="ACFB2Q_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC0917898.1"
                     /translation="MKTGIQTELAQALLSHEKIWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPASQPASQPASQPASQPASQP
                     ASQPANYTPN"
     gene            7459..8919
                     /locus_tag="ACFB2Q_000846"
     CDS             7459..8919
                     /locus_tag="ACFB2Q_000846"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC0917899.1"
                     /translation="MIYIDPPYNTETDSFAYNDKFSHSTWLTFMKNRLEIAKELLKDD
                     GLIFVQCDDKEQAYLKVLLDETFTRENFINCIAVKMSEPSGNKMAHTSHRLPKIKEYI
                     LIYKNKNIKLNPIREQKSEWDNEYNIFLENFTQEDKKFIDLIVNSQTENKEINGNTLK
                     EIDILLKKISPISVNQKLAQLNIKDNEVIKWKLDNAYRIVRTAASSSVKKLADEKKEI
                     CQQQFFSVISKRDKLLYIVKSDYSKDAKAPRVQVLFAEDYLSISLCDLWTNINTTGLE
                     AEGNVELKNGKKPESLIETIIKLATNENDIVLDYHLGSGTTAAVAHKMNRQYIGIEQM
                     DYIETLAVERMKKVIDGEQGGISKAVNWQGGGEFVYAELSPFNETAKQQILACENSDG
                     IKTLFEGLCERHFLKYNVSVNEFSQIIEEPEFQSLALDEQKQMMLEMLDLNQMYISLS
                     EMDDEQFAGCLNDDDKALSRAFYQSVKHQAEKKDGE"
     gene            8909..11683
                     /locus_tag="ACFB2Q_000847"
     CDS             8909..11683
                     /locus_tag="ACFB2Q_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC0917900.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMIAALILYYFEKGYRH
                     FLFFVNKNNIVYKTENNFIDPTHPKFLFTEKILQGDTVIPIRKVETFSQYSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEKEMND
                     RTSDAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENTEVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYLAFLNWVENVQADDFSFLTTFSTSLNDSNNANEQGKTRTEQALKFMQENK
                     FEFVHLANWIKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLD
                     IQNRNELQNKLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFI
                     EPKGGHLVENDSWKEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTEL
                     FPLGAASLEK"
     gene            complement(11832..13004)
                     /locus_tag="ACFB2Q_000848"
     CDS             complement(11832..13004)
                     /locus_tag="ACFB2Q_000848"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC0917901.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(13125..13367)
                     /locus_tag="ACFB2Q_000849"
     CDS             complement(13125..13367)
                     /locus_tag="ACFB2Q_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917902.1"
                     /translation="MVSLMVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSL
                     ILLYLFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            13354..13800
                     /gene="iscR"
                     /locus_tag="ACFB2Q_000850"
     CDS             13354..13800
                     /gene="iscR"
                     /locus_tag="ACFB2Q_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC0917903.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            13829..15043
                     /locus_tag="ACFB2Q_000851"
     CDS             13829..15043
                     /locus_tag="ACFB2Q_000851"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC0917904.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            15028..15339
                     /locus_tag="ACFB2Q_000852"
     CDS             15028..15339
                     /locus_tag="ACFB2Q_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917905.1"
                     /translation="MGGALRLMSNKGCLRKNTMKVKIINLPNGYKRIIYGKYFEQFDL
                     DYEQDLDVLKKDIEFALSVIEYNRSIFKKFSSLFENKIIFVYQGGHHLDIIDRDKGSL
                     K"
     gene            15672..16139
                     /locus_tag="ACFB2Q_000853"
     CDS             15672..16139
                     /locus_tag="ACFB2Q_000853"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HFC0917906.1"
                     /translation="MAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFANFAFIENES
                     RKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLFMTQLAIKNG
                     FQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQTTIF"
     gene            16152..16538
                     /gene="iscU"
                     /locus_tag="ACFB2Q_000854"
     CDS             16152..16538
                     /gene="iscU"
                     /locus_tag="ACFB2Q_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC0917907.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            16625..16945
                     /gene="iscA"
                     /locus_tag="ACFB2Q_000855"
     CDS             16625..16945
                     /gene="iscA"
                     /locus_tag="ACFB2Q_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC0917908.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(16905..17126)
                     /locus_tag="ACFB2Q_000856"
     CDS             complement(16905..17126)
                     /locus_tag="ACFB2Q_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC0917909.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            17208..17708
                     /gene="hscB"
                     /locus_tag="ACFB2Q_000857"
     CDS             17208..17708
                     /gene="hscB"
                     /locus_tag="ACFB2Q_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC0917910.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            17806..20556
                     /gene="gyrA"
                     /locus_tag="ACFB2Q_000858"
     CDS             17806..20556
                     /gene="gyrA"
                     /locus_tag="ACFB2Q_000858"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC0917911.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDFAVYATIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
BASE COUNT         5924 a         5210 c         4787 g         4834 t
ORIGIN      
        1 ccttttccgc tttggcaacg attgaaaatt tccgtaaatt caaatatcta gattccttcc
       61 tgcgtgggaa tgacgcagaa gggtttcaga tgcaggatgg gcattcctgc ctacccaatc
      121 tcgccctacg gcttgacggt tcgatatgat gccgtctgaa aacccaacgg cggtatgaca
      181 atgccgccct gccaacgcac gtagatcaga attgccatcc cgacatcaaa cgcttggaaa
      241 caaaatgccg tctgaaaatc aaacggcaac ataacaatgc ccctaacaaa tacaaaaatg
      301 ccgtctgaaa gcccttcaga cggcattggc gcgccgggtt taccgccttc tgccgaaacc
      361 gcgcatagcg ggacggcggt aattgacggg cgggcgcgtt gtcgggcggt aacgctgcgc
      421 ctgcgccgcc tgttgttttg cacggaggct gcgcgtgttc aaatccctgc tggtgcgcgc
      481 attggggcgt gcggactgat ggtaggcagc acgcgcgcga cgggcgacgg ggctgtcttg
      541 gttgcccgcc cgtgtgaatt tgtttgccag cgcgttgccg ataaacgcgc ctgccgccgc
      601 gccaatcagg ctttgcagca gccagcttcc tgtcgattgg tcgtaaatat actgctgccc
      661 gtctttgccg gtaacgggct gaccgttgtt gccgtttgcc tgcgcctggg caggaatggt
      721 gtctttgact gcttcgggag tcagttggta aaccgtatcg tctgcctgct gtgcgagctg
      781 ctgttgcagg gcttcaatct gtttctgctg ccgttcgagc cgcgcctgcg tgtcgtcttg
      841 gcaggcggtg agtgcgaatg ttgcgataag cgcggaggcg atgatttttt tcatgtgtgt
      901 cctgttcggg tggaaaatcg gttgtattgt atcgccgtcg ggaattttgg caagcattct
      961 gccggcaaat cgtgatgttt acaggggtag ggtgtgcagt ttgcggacaa atgcgaggct
     1021 gttggcgaca gggttgcctt tgttttcgac ttcgtgttcg gtttctatgg tcagcaggcg
     1081 gcggtttttg tgtttgttca ggctcaattc ggcttgtgcg ggtgtgaaaa acaggcggtg
     1141 tttttcggca aagcggcagg cgcgcttgtt tgcggttttc aaaatctgaa gcagcgatac
     1201 gtccagatgg aagcgtcgca cgcccaatac gagaatccgt gcggcaaaaa aatcggcggc
     1261 atcggtaaac tcggcggggc tgctgcggct gaagtcgtgc cgttcggcgg ctatcagggc
     1321 ggcaacggtg cggatttgcg gaatcatcga ttcgctgatg agcgtgtcgt cccgccctgc
     1381 atcgagaagc atgggggaaa aatcctgcgc atcatcgaca ataatgctgc aagtgtgcag
     1441 gttttcgttt tgtacggcgg tttggggcat tgcatccatg gtcattttcc tgattctgtc
     1501 gtgtgttgcc gaatcgggcg acctgtgtga aggtaacaaa aaagccgccc cgttttcgag
     1561 cggcctgttt tgcgtatggg atggatttca agcaagcgca aaaaagcacc gcacgtctgt
     1621 gtggtaccaa tagcagtaag cggttgtaaa cgttttgcct tgcatgatga aatgccgtcc
     1681 gaagataaaa atattgggga gattctaaat caaaacgctg ccgcgcctca agcattatat
     1741 cgaaattttt ttgttttttt cagctatcct gttgaaaata tttaggttta tttttaccac
     1801 tgcccgatat tgtcggcaat ttccctttat ccgccttgaa aaacggtgca taatcccgaa
     1861 caaaaccgca atcaggagca attatgcaaa actatctgac ccccaatttc gcctttgccc
     1921 cgatgattcc cgaacgcgct tcaggcagcc gcgtttggga tacggaaggg cgtgaatata
     1981 ttgatttttc aggcggcatc gccgttaacg ctctggggca ctgccaccct gcccttgtcg
     2041 atgctttaaa cgcgcagatg cacaagctgt ggcacatttc caatatctat acgacgcggc
     2101 cggcgcagga attggcgcaa aaattggtga aacacagttt tgccgacaag gtttttttct
     2161 gcaactcggg cgcggaagcg aatgaggcgg cgttaaagct ggcaaggaaa tatgcgcgcg
     2221 accgtttcgg cggaggcaaa agcgaaatcg tcgcctgtat caacagtttc cacggacgca
     2281 cgctgtttac cgtgtccgtc ggcggacagc cgaaatacag taaggattat gcaccgctgc
     2341 cgcaaggcat tacgcacgtt ccgttcaacg atattgccgc gctggaagct gccgtcggcg
     2401 aacagacctg cgcggtcatc atcgagccga tacagggcga aagcggcatc ctgcccgcca
     2461 ctgcggaata tttgcaggcg gcgcgccgtc tgtgcgaccg gcacaatgcg ttgttgattt
     2521 tggacgaagt tcaaaccggg atggggcata cgggcaggct gtttgcctat gaacattacg
     2581 gcgttgttcc cgatattttg agttcggcaa aagccttggg ctgcggcttt ccgatcggcg
     2641 cgatgctggc gacagaaaag attgccgccg ccttccaacc gggcacgcac ggctcgactt
     2701 tcggcggcaa cccgatggcg tgtgcggtcg gcagccgcgc cttcgacatc atcaacgcgc
     2761 cggaaacctt gcacaacgtc cgcagtcagg ggcagaaact tcagacggca ttgctggatt
     2821 tgggcaggaa aacaggcttg ttctcacagg ttcgcgggat ggggctgctg ctcggctgcg
     2881 cgttggacac gccttatcgc ggacgctcat ccgaaatcgc cgctacctcc ttgaaacacg
     2941 gcgtgatgat cttagttgcg ggtgcggacg tattgcgttt cgcgccttcg ctgctgttga
     3001 acgatgagga tattgcggaa ggtttgcggc gtttggaaca cgtgctgacg gaatttgccg
     3061 ccgcaaaccg cccctaggca gaaagcagat gccgtctgaa gacgggaagg cctcagacgg
     3121 catcagaaac aaaaaaccgc ttcggaaacg tgatccaacg ttccgaagcg gttttgtttg
     3181 ccatcaggac tcgaacacca attccggttc cctgccctct tcgatgactg ccttgccgac
     3241 caccactttt ttcaagcctt gcaaatcggg caggcggtac atcgtatcga gcaggcagcg
     3301 ttcgacgatg gaacgcaggc cgcgcgcgcc ggttttgcgt tccattgcct gccgcgcgat
     3361 ggaacgcaat gcgccttctt caaattccaa cccgacgttt tctatgccga acaaggcttg
     3421 atactgcttg accaaagcgt ttttcggctc ggttaaaata ttaatcagcg cgtcctcatc
     3481 cagttcttct aaagttgcaa tcacgggcaa acgtccgatt aattccggaa tcaggccgaa
     3541 tttgattaaa tcttccggtt cgacgatgcc gaacagcttg gtaatgccgg cattttcgtc
     3601 cttgctgtga acggacgcgc cgaaaccgat accacctttc tcggtgcgct ggcgaatcac
     3661 tttttccaag cctgcaaacg cgccgccgca gataaacagg atgttggcgg tatcaacgtt
     3721 gataaattcc tgattcggat gcttgcgtcc gccttggggc ggaacgcttg ccaccgtacc
     3781 ttcaatcagt ttcagcaagg cttgctgcac gccttcgccg gacacgtcgc gcgtgatgga
     3841 cgggttgtcg cttttacgcg aaattttgtc aatttcgtca atatagacaa tgccgtgctg
     3901 ggctttttcg acatcgaaat cgcatttgcc caacagtttg gtaatgattt gttcgacatc
     3961 ttcgccgaca tagccggctt cggtcagcgt ggtcgcatcc gccatcacga acggcacgtc
     4021 cagtttgcgt gccaaagatt gcgccaacag cgttttaccc gatccggtcg ggccgataag
     4081 caggatgttg gatttcgaca attcgacacc gccgtcggct ttcgggtggc gcaggcgttt
     4141 gtaatggttg taaaccgcaa ccgccagcgc ctttttcgcc tgcccctgcc cgatgacata
     4201 atcgtcgagg ttggcaacga tttcggcggg cgtgggcagt ttgccggatt cttccggctc
     4261 ccctccggca ttttccggag gcgtgccgcc gttgtcgcct tcgtacaata tttcgaggca
     4321 gtttgcgacg cattcgtcgc agatataggc gtttttgccc tcaatcaggt gtttggcgtg
     4381 tgatttggat tttccgcaaa aggaacaggt acggttttcg ttggacatgg ctttctttac
     4441 aatgtatgcg ttgcagaaac ggcatgcgcc gttcgggttg ccaagtataa taactatatc
     4501 cgtgcttatc aacgtattac cttaaaatcc cgccgattcg gctataatac gccctttcgc
     4561 aaccgccccg gcggcagaaa tgccgtctga caccaaatct gaggatattc atgagaaaac
     4621 cccaacgcgg ctacgcccgc caagaccgtg tcaaagaaca aattatgcgc gagcttgccg
     4681 aactcgtccg taccggactg aaagacccgc gcgccggctt catcactatc aacgaagtcg
     4741 aagttacccg cgattacagc cacgccaccg tgttctacac cgttttgaac caagatacgc
     4801 gcgaaattac ggaagaagtg ctggaacacg cgcgcggaca cctccgcagc gaattgtcca
     4861 aacgcatcaa gctgttcaaa atccccgaac tgcatttcaa atacgacgaa tctttggaac
     4921 gcggtatgag cctgtccgcc cttatcgatc aagtggcggc ggaaaaaccg gttgaagact
     4981 gatactgcca gaatgaacaa tctgattccc gaacatttag ccgcctatgc acatagtgat
     5041 aacctgcaaa ttgaaggcgg gcatcgttgc ttttcattat cctgccaagg tagagatact
     5101 ttccacatcc gttactatgg agagcctttt gatggattga ttaccgatac tgataaggcg
     5161 ccggtaaaaa ttgtggcggt agaagctgta agcggcgatg aaatcgtatt gtttgatggg
     5221 gcggaacatg gctataacgc tatgttctgc gacaaatata gccaaaatca aaagcaaaac
     5281 agaacgttaa ctgatttgga tgaatatact taccgagttc cgattcatct ttattacaat
     5341 atagactacg aagatgaata tgaagatttc gtcaattctg aaggacaagt ccccttaatt
     5401 gatggccgca tcattagttt tgactcatta aaacgaaatg gctttgatgc aatcagcatt
     5461 gatctaattg atgaaaaaca ctctgttcgt gaattattga atgaagaatt aagctgacac
     5521 gcaggccgtc cgaacccttt cagacggtct gaacattaat attccctaat taatatgacc
     5581 aataaacccg ccaaacgccc ggtcaacggc gttcttcttc tcgacaaacc cgaaggcctt
     5641 tccagcaaca ccgccctgca aaaagcgcgg cgtttgtttc atgccgaaaa agcagggcat
     5701 accggcgtgc tcgatccttt ggcaaccgga cttttgcccg tctgcttcgg cgaagcggcc
     5761 aagttcgccc aatacctgct ggatgccgat aaagcctaca ccgccacgct gaaactcggc
     5821 gaagccagca gcacgggcga tgccgaagga gaaatcatcg ccgccgcccg cgccgatatt
     5881 tccttagccg aatttcagac ggcctgccaa gcactgacag gcaacatccg ccaagtgccg
     5941 ccgatgtttt ccgccctcaa gcacgaaggc aaaccgttgt acgaatacgc gcgcaaaggc
     6001 attgttatcg aacgcaaacc gcgcgacatt accgtttact ctatcgatat tgccgagttt
     6061 gacgcgccca aagccgtgat aagcgtgcgt tgcagcaaag gcacctacat ccgcaccctc
     6121 agcgaaggca tcgccaaaca catcggcaca ttcgcccacc tgaccgccct acgccgcacc
     6181 gaaaccgccg gctttaccat cgcccaaagc cacacgctcg aagccttggc aaacttggac
     6241 gaaacagaac gcgacaacct gctgctgccc tgcgacgtat tggtttcaca ctttccccaa
     6301 accgttttaa acgattatgc cgtccatatg ctccaatgcg ggcaacggcc gcgtttcgag
     6361 gaagacctgc cttccgatac gccggtacgc gtttacacgg aaaacggccg ctttgtcggt
     6421 ttggcggaat atcaaaagga aatatgccgt atgaaagcct tgcgcctgat gaacacggcg
     6481 gcatcctccg cctgagcggc ggttaaaaat acgggctgcg tttgaataat gtgttgatat
     6541 ttccgtaaaa tcacgacaca ctcggacacc cgccccgctt atcgcaactt tgcgaacttc
     6601 ccccggaaac agcaaagaca tcaaagaatt gattttatta taatttattt gtaaagccat
     6661 ttgccgttac acaagaatgg cacattaaaa taactgatga ggatttataa cgatgaagac
     6721 aggcattcaa accgaattag cccaagccct gctatcacac gaaaaaatat gggcgaacga
     6781 agaaaaaacc attttggcca aaaacatcct gttggatttg gtggaaaaaa ccgacccgtc
     6841 cattatcggt ttgttattga ataatgatga gttaaaacgc catttctttg tggaagtgaa
     6901 tggtgttttg gtgtttaaat tgcaggattt ccgttttttc ttggacaaac acagcatcaa
     6961 taattcctac acaaaatacg ccaaccgcat tggtttgacg gacggcaacc gctttttgaa
     7021 agacaattcg gatattgtgt tggattttcc gtttaaagat tgcgtattga atggcggaca
     7081 aagcaccgaa gaaggcgaag aaatttattt taaacgcaat aatagccagc cagccagcca
     7141 gccagccagc cagccagcca gccagccagc cagccagcca gccagccagc cagccagcca
     7201 gccagccaat tatacaccaa attaaccaga aaaagacaag aaatcttttt taatcaaacg
     7261 cttgcttttg atgaaattga ccggcttttt gacgcaaaag cattctcaaa attctcccgc
     7321 cacaccgcag acggcaaaca acccgttggc gaaatcaaac gccgttcaga cggtacgcca
     7381 gccgaaaatc ttattatcaa aggcaataat ctgattgccc tgcattcgct tgccaagcag
     7441 tttaaaggca aagtaaagtt gatttatatt gatccaccgt ataatacaga aaccgatagc
     7501 tttgcatata acgataaatt ttcacattcc acttggctaa catttatgaa aaatcgcttg
     7561 gaaattgcga aagagttatt aaaagatgat gggctaatct ttgttcaatg cgatgataag
     7621 gaacaggctt atttaaaagt tttattagat gaaactttta caagagaaaa ttttataaac
     7681 tgcattgctg taaaaatgtc agagccatca ggtaacaaaa tggcacatac atcgcataga
     7741 ctaccaaaga taaaggaata tattcttatc tataagaata aaaatataaa attaaaccct
     7801 attagagagc aaaaatcaga atgggacaat gagtacaata tttttttaga aaattttaca
     7861 caagaagata aaaaatttat tgatttaata gtaaatagcc aaacagaaaa taaagaaata
     7921 aatggtaata ctttaaaaga aattgatatt cttttaaaga aaatatctcc tatttcagtt
     7981 aatcaaaaat tagcccaatt aaatattaaa gataatgaag ttataaaatg gaaactagat
     8041 aatgcgtata gaattgtaag aacagcagct agtagtagcg taaaaaaatt agcagatgaa
     8101 aaaaaagaaa tttgccaaca acaatttttt tcagttattt ctaaacgaga taaattattg
     8161 tatatcgtca agtcagatta ctcaaaagat gcaaaagcac cacgagtgca ggttttgttt
     8221 gcagaagatt atttgagtat tagtttatgc gatttatgga caaatataaa taccacagga
     8281 ctagaagcag aaggaaatgt agagttaaaa aatggtaaaa agccagaaag tttaattgaa
     8341 accatcatta aactagcaac caacgaaaac gacatcgttc tagactacca cttgggcagt
     8401 ggcacaaccg ccgccgtcgc ccataaaatg aaccgccaat acatcggcat tgaacaaatg
     8461 gattatattg aaacgcttgc cgttgaacgc atgaaaaaag taattgacgg cgagcaaggc
     8521 ggtatctcaa aagctgtgaa ttggcaaggt ggcggcgagt ttgtttatgc cgaactttcc
     8581 ccatttaacg aaaccgcaaa acaacaaatt ttggcgtgcg aaaattcaga cggcatcaaa
     8641 acgctgtttg aaggtttatg cgaacgccat ttcttgaaat acaacgtcag cgtaaatgaa
     8701 tttagccaaa ttattgaaga gcctgaattt caatccttgg cattagacga acaaaaacaa
     8761 atgatgcttg aaatgttgga tttaaatcaa atgtatattt cattatccga aatggacgat
     8821 gaacaatttg caggctgcct gaatgatgat gataaagcgt taagccgtgc gttctatcaa
     8881 tcagtaaaac atcaagcgga gaaaaaagat ggcgaataat aaaacgttgt ttgattgggt
     8941 ggaagaccgc aaatcaatgc tggaagaaat ggaacagacg gattttttcg cgctgcctga
     9001 atttgtttcc aataatttaa aatatccgtt ttttgaatgg caaaaatcgg ctttagaaaa
     9061 ttttgtgatt tttgaccgca cttcaaaatt aaaggatttc cccgacatca aaaaccgccc
     9121 tacccatttg ctgttcaata tggcaacagg tgcaggcaaa acgatgatga tagcggcatt
     9181 gattctgtat tattttgaga aagggtatcg gcatttttta tttttcgtca ataaaaacaa
     9241 tatagtatat aaaacggaaa ataattttat cgacccgacg catccaaaat ttttatttac
     9301 cgagaaaatt ttgcaaggtg atacggtaat tcccattcgc aaagtggaga catttagcca
     9361 atattcagac ggcattgaaa ttaaatttac cagcattcaa aaactatata acgatattca
     9421 caccgagcgg gaaaatcaaa ccacattggc ggatttgcac gaattaaatc ttgtgatgct
     9481 gggcgatgaa gcacaccatt taaacgcgca aaccaaaggc aaaaaacaag gcgaattaga
     9541 tttagaaaag gaaatgaacg accgtaccag cgatgccgaa attgaacgca agggttggga
     9601 gcatatggtt ttggagttgt tactcaataa aaatggcaat cccggccaaa atgtgctgtt
     9661 ggaatttacc gccacgctgc ctgaaaatac tgaagtgcaa caaaaatatg ctgataaaat
     9721 catcacaaaa tttggtttaa aagaattttt gcaaaaaggt tataccaaag aaatcaattt
     9781 ggtatccggt acgctgaata agaaagagcg ggtgttgcac gctttattgt ttgcctggta
     9841 ccgccatcaa atcgcgttga aatacggcat tgccaatttc aagcctgtga tgttgtttag
     9901 aagtaagacg attgatgaat caaaagcgga ttatttggca tttttaaatt gggtagaaaa
     9961 tgtgcaagcg gatgattttt cgtttttaac tacattttca acaagcttaa acgatagcaa
    10021 taacgccaac gaacaaggca aaacccgtac cgaacaagcc ctgaaattta tgcaggaaaa
    10081 taaatttgaa tttgtgcatt tggcaaattg gattaaacaa aattatcaaa aacacaatgt
    10141 cattattacc aactccgaaa ccaacaaaaa caaaactgaa aaaaccgaca gcgaaaccga
    10201 aaaattgctg aacaatctgg aagcggctga taatccgatc cgtgccattt ttacggtgga
    10261 tagattaacc gaaggttggg acgttttaaa tttgtttgat attgtgcgtt tgtatgaagg
    10321 gcaaaacggt ggcggttcga ataaaaaatc aggcaaaacg gctgccgcca ccgtatcgga
    10381 aaagcagttg attggtcgcg gcgtgcgtta ttttccattt gcgtttgaag gtaaacagcc
    10441 gaataaacgc aaatttgaca acgatatgca acatgaattg cgtatcttgg aagaattgtt
    10501 ttattacacg cacgatgagc aatcccgcta tattacagaa ctgaaaaacg agttacgaaa
    10561 agacggttat ttgcctgaaa aagacgatga taaggtattg gcaacattta agctcaaatc
    10621 tgaatttgca gataataagg attttagaga gttgttaatt tgggcgaata aaaaaatccc
    10681 caacccaaac gccaaaagca ataatgcaga cagcctgcaa gccaatccgc aaacgctttc
    10741 attccaagtt cacggtaatc aactgttgca ggaaacgcaa tttacagccg atgaaaatga
    10801 tgaaacagcc cgacaaatcg gcacacaaaa taattttact caaaccataa aaatgagtga
    10861 aatggagcgg catattttca ataaagcatt acatatcaaa gggaaaaata gtcaatcttt
    10921 attccatttt gaccgcctgc aaagcaaact cgacattcaa aatcgcaatg aattgcaaaa
    10981 taaattgttg aaagactggc agattgaatt tttgggatta gagcaagata aacaggttcg
    11041 cccggatgat aaacttgcag gctgcctgaa aatcttggaa atggttgaaa aacatctgaa
    11101 tgaaagtgat ataccgttta tcggtacaaa agaatttaca cctaaaaaat tgtgggaaat
    11161 tttcggcacg ccaaaacaaa aatgggtcaa aaaagatgat ataaaaactg ctattgccac
    11221 gcaaaatgat tggtatgtga tggataattt tgccggaacg ggtttggaag aagcgttaat
    11281 tcaatttatt tcagcgcgct tgggcgattt gaaatctcaa tatgacgttc atttaatccg
    11341 caatgaagaa gtgtttaaat tgaataactt tgccgatggc gaaggattta tgccggactt
    11401 tgttttattg ctgaaagata aacaaaaatc ttcttccgac agcgtggacg actttttgca
    11461 ttaccaaatt ttcattgaac cgaaaggcgg gcatttagtg gaaaatgatt cgtggaaaga
    11521 agctttttta aaatcaatca cagtggaata tgggagggat aagatcctgc aaaaaaatac
    11581 accgcattat cgtttgatcg gtttgccgtt ttttactgat catcagaaaa atggacaatt
    11641 tacagagtta ttccctttag gtgcggcatc gcttgaaaaa tagagtagtg cattgcaggc
    11701 agccccgttc ggcaaaactt cctttacaaa agggcgtttt gtcagatatt taatcaacac
    11761 attattaaaa tacaaccaaa ttttaatgcc gtccgaacca tgtattcaga cggcatcgta
    11821 tttttcagta tctaaaccgt ttccctgccc caatccttac tcctcaaaat cgaggcatcg
    11881 acatcttgaa tatcgcggtg tcccgtaaac gccatcgaca catccatttc cttgtacaaa
    11941 atctccagcg cgcgcgttac gccttcttcg ccatacgcac ccaaaccata caggaacgcc
    12001 cgacctatca tcgtgccttt cgcacccaaa gcccacgcct tcaaaatatc ctggccgctg
    12061 cggataccgc tgtccatcca aacttcgatg tcactgccga ctgcggaaac aatatcgggc
    12121 aaggccttga tggcggacac agtatcgtcg agctggcggc cgccgtggtt ggaaacgacc
    12181 aatgcatccg caccgctttt cgccgccttt tccgcgtctt cgggttccat aatgcctttg
    12241 ataatcagct tgccgcccca caaatcttta atccgcgcca cgtcgtccca gcttaagcgc
    12301 gggtcgaact gttccgccgt ccacgaagaa agtgaagaca aatcgccgac gtttttggcg
    12361 tgtccgacga tattgcggaa cgtgcggcgt tccgtgttca gcattttcat gcaccattcg
    12421 ggcttggtcg ccagattgat taaattggcg atggtcggtt tcggcggcgc ggacaggccg
    12481 tttttgatgt ctttgtggcg ttgacccaaa acctgcaaat cggcagtcaa taccaatgcc
    12541 gaacatttgg catctttcgc acgcttaatc aggttttcca taaattcgcg gtcgcgcatc
    12601 acataaagct gaaaccaaaa cggcgcgctg gtgttctcgg caacgtcttc aatcgagcag
    12661 atggacatgg tggacaacgt aaacggaata ccgaacttct ccgccgcacg cgccgccaaa
    12721 atttcaccgt ctgcgtgtgc catgccggta aaacccgtcg gcgcaatcgc caccggcatt
    12781 ttcacatccc ccccgatcat tttggtttcg aggctgcgtc cctccatatt gaccaatacc
    12841 ttttgtcgga agcggatatc tttgaaatcc gaagtattct ccctataagt cgtttccgtc
    12901 cacgaacccg aatcgatgta atcgtaaaac atacgcggca ttttgcgctt ggcaacgcgg
    12961 cgcaagtctt cgatgcaggt cattttgctc aaatcacgtt tcatcttgtt tttccctgtt
    13021 taacagcctg atggaatatg ccgtctgaaa ctgcttgagc cttcaaacgg catttcgggc
    13081 aatacccaaa taaatcacta agatttagat aatctatatc aatattaaaa ataaatcaaa
    13141 tcgttttgaa accgcttgcc ggcaaaacgc cccaaccgcc gctcctcaaa aaacataact
    13201 aactgattca aaaaaccaaa caaataaagc aaaatcagac tataccgcca tttaaacaaa
    13261 caatcggcaa cagctccccc atacttgact gaaacactca gatattggac aattccgccc
    13321 actataaaaa agccgacacg ggcaaccacc accatgagac tgaccaccaa agggcgtttc
    13381 gccgttaccg ctatgctgga tttggcgatg aacgcgcaaa ccggcgccgt caaactcagt
    13441 gccatcagcg aacgccaaaa tatatccctc tcctatctcg agcaattgtt cggcaaactc
    13501 cgccgcgccg gactggttga aagcctgcgc gggcccggcg gcggctacat cctcgccgcc
    13561 cctcccgcac aaatcaacat cgcccaaatc atttccgccg ccgaagaccg tctggacgcg
    13621 acccaatgcg gcagcaaagc caactgccac cacggcgcgc cctgcctgac gcacgacctt
    13681 tgggagaatt taaacaaaac catcaacgac tatctcggca gcgttaccct gcaaagcatc
    13741 atcgaacaga aaaacaacgg cgacggcagc cgcgtcgtcc aatttacaca catccattaa
    13801 ataacacccg aaaaagaaag agcaaaccat gaccgtcaaa acccccgttt acctcgacta
    13861 cgccgccacc acacccgttg acaaacgcgt tgccgaaaaa atgattccct atctgactga
    13921 aaccttcggc aaccccgcct ccaacagcca cgcattcggc tggacggcag aagaagccgt
    13981 cgaaaaagcc cgcgccgaca tcgccgccct gattaacgcc gaccccaaag aaatcgtctt
    14041 caccagcggc gcgaccgagt ccgacaacct cgccatcaaa ggtgcggcaa acttctacaa
    14101 aaccaaaggc aaacacctca tcaccgtcaa aaccgaacac aaagccgtgc tcgacacgat
    14161 gcgcgaactc gaacgccaag gctttgaagt tacctacctc ggcgtgcagg aaaacggttt
    14221 gattgatttg gaagaactca aagccgccat ccgcgacgac accatcctga tttccataat
    14281 gtgggtgaac aacgaaatcg gcgtggtgca aaacattccc gccatcggcg aaatctgccg
    14341 cgaacgcaaa atcgccttcc acgtcgatgc cgcccaagcc tgcggcaaag tgcctgtcga
    14401 tgtcgaagcc gccaaaatcg acttgctctc gatgtccgcg cacaaagtgt acggccccaa
    14461 aggcatcggc gcgctgtacg tccgccgcaa accgcgcgtc cgcctcgaag cccaaatgca
    14521 cggtggcggt cacgagcgcg gtttccgttc cggcacattg ccgacccatc aaatcgtcgg
    14581 catgggcgag gccttccgca tcgccaaaga agaattggca caagacacag cgcactacct
    14641 gaaactgcgc gacatcttcc tcaaaggcat cgaaggcatc gaagaagtct atatcaacgg
    14701 cgacctcgaa caccgcgccc cgaacaacct gaacgtcagc ttcaacttcg tcgaaggcga
    14761 aagcctgatt atggcggtga aagaactcgc cgtatccagc ggatccgcct gcacctccgc
    14821 ctcgctcgaa cccagctacg tcctgcgcgc gcttggccgc aacgacgaac tggcgcactc
    14881 atccctgcgc atcaccttcg gccgcatgac caccgaagaa gaagtgcaat tcgcggcaga
    14941 actgatcaaa tccaaaatcg gcaaactgcg cgaactgtcg ccgctgtggg aaatgttcaa
    15001 agacggtatt gatctgaact cgattgaatg ggcggcgcat taagattgat gtcaaacaaa
    15061 ggctgtctga gaaagaatac catgaaagtt aaaattatta atttgccaaa tggttataaa
    15121 cgtattattt atggaaaata ttttgaacaa tttgacttag attatgaaca agatttagac
    15181 gtattaaaaa aagatattga atttgcttta tctgttattg aatacaatcg atctatattc
    15241 aaaaaattct cttccttatt tgagaataaa atcatttttg tttatcaagg aggacatcat
    15301 cttgatatta ttgatcgtga taaaggaagt ttgaaatagt taactattga agatcattgg
    15361 acaaaagatg ttgatgatat cactggttca tggaaaatgg agaatttaga aattagccga
    15421 caaactcaat atgcaatgca acttctaaag attggtaaaa caaatattga gaaagaaata
    15481 agaaaaatta aacaaaggtt ctaaaaaaat gagtaaatac ggtggtgaag acgtaatcta
    15541 tatgaatgat ggtgtattta gaaataaatt agccattcaa gatccactta aatttaaaaa
    15601 aggtcgaaag agacatttct gctatttccg cccaaaatct gcttttgcaa cccattacag
    15661 gcaatttcga tttggcgcat ttacagacca ttcatcgcga actgtttggc aatgtttatg
    15721 attgggcagg aaaaatccgg agggttgata tttctaaagg taatacccga tttgccaatt
    15781 ttgcttttat cgaaaacgaa agccgtaaac ttttggagaa gctgaaaaac gaaaactatt
    15841 tgcgggtgtt ggataaagat aaattcgccg agcgtgcagc ctattatttg gacgagttga
    15901 acgtcctaca tccttttcgt gaaggaaacg gcagaacatt gcgcctgttt atgacacaat
    15961 tggcaataaa aaacggtttt cagatacatt ggcaaaatat ctctgccgaa caaatgattc
    16021 aagcctgtat tcaggcatac catgccgata gcagcttatt ggcacgccta attattagac
    16081 aatttagaac aatcgtcttt ttcagacgac taaaacatca acaaaccacg atattttaag
    16141 gaaaccacat catggcatac agcgataaag taatcgacca ctacgaaaat ccccgcaacg
    16201 tcggcacttt cgacaaaaac gacgagtccg tcggcaccgg catggtcggc gcgcccgcct
    16261 gcggcgacgt gatgcgcctg caaatcaaag tgaacgatga aggcatcatc gaagatgcga
    16321 aattcaaaac ttacggctgc ggttccgcca tcgcttcgtc cagcctgatt accgagtggg
    16381 tcaaaggcaa aagtctggat gacgcgctgg caatcaaaaa cagcgaaatc gccgaagaac
    16441 tggaattgcc gccggtaaaa atccactgct ccatcttggc tgaagatgcg gtaaaagcgg
    16501 ccgttgccga ctaccgcaaa cgtcaggaaa acagataaag cccttcagac ggcatcgtcc
    16561 cacaatgccg tccgaaccac ccgccgcttc ggatccgtcc ggggcggtac aacaaggaag
    16621 aaatatgatt acccttaccg agaatgccgc aaaacacatc aatgactatc tcgccaaacg
    16681 cggcaaaggc ttgggcgtac gcttgggtgt aaaaaccagc ggctgctcgg ggatggcgta
    16741 caaccttgaa tttgtcgatg aagccaacgg cgacgacctg attttcgaag gacacggcgc
    16801 gcgcatttat atcgacccga aaagcttggt ttatctggac ggcacacaag tcgattacac
    16861 caaagaagat ttgcaggaag gtttcaaatt tgaaaacccc aatgtcaaag actcctgcgg
    16921 ctgcggcgag agcttccacg tttaaggcat aaaaacggcg ggacagtatc aaaaccgtcc
    16981 cgccgttttt tcgcttcctg cctgttatag ctgcctttgc ctttcctttt ccgttccacc
    17041 ttgtgccgga acaaatcgga ttttactaag gcttttaaag cattgtcgcg tattttgcct
    17101 ttattgtgct gcgctttgcc gcccatattc agtcctttcg tttaagaagc cgcagattat
    17161 aaggcaaaaa cagttttctg ccaaaatctt acatttatca tcctactatg tcccaatatt
    17221 tcaccctctt ccggattgaa cccgctttcg atatcggcac cgaaaacttg gaacaaacct
    17281 accgcgcctt ggccgcccgt ttccatcccg acaaattcgc ttccgcttcc gctttcgagc
    17341 agaaacaggc ggttatgatg tcttccacca tcaacgatgc ctaccgcacc ttgaaaaatc
    17401 ccatcgaccg cgccgcgtac ctgctgaaaa catcgggtat cgatgccgac gcgccggaac
    17461 atacctcttt cgcccccgac ttcctcatgc agcaaatgga atggcgcgaa acgctgatgg
    17521 aggcacgggc aggcaacaac cttgaatcct tgaaaaatct cgataacgaa atccgcgccg
    17581 aacaagaaaa actgttctgc ggtctgaaac agtcattcgc gcgccaagac tgcgacaccg
    17641 ccgcacaaca agtccgccaa ggcaggtttc tcgacaaact ccgccacgaa atttcctccg
    17701 cattataatc cgcaccgtgt ttcagacggc ataaccgccg caccgtttca tgtcgaaata
    17761 tgctaaaata agcaacaatt ttttgccata cgaaacattg aaaccatgac cgacgcaacc
    17821 atccgccacg accacaaatt cgccctcgaa accctgcccg tcagccttga agacgaaatg
    17881 cgcaaaagct atctcgacta cgccatgagc gtcattgtcg ggcgcgcgct gccggacgtt
    17941 cgcgacggcc taaagccggt gcaccggcgc gtactgtacg cgatgcacga gctgaaaaat
    18001 aactggaatg ccgcctacaa aaaatcggcg cgcatcgtcg gcgacgtcat cggtaaatac
    18061 cacccccacg gcgatttcgc agtttacgcc accatcgtcc gtatggcgca aaatttcgct
    18121 atgcgttatg tgctgataga cggacagggc aacttcggat cggtggacgg gcttgccgcc
    18181 gcagccatgc gctataccga aatccgcatg gcgaaaatct cacatgaaat gctggcagac
    18241 attgaggaag aaaccgttaa tttcggcccg aactacgacg gtagcgaaca cgagccgctt
    18301 gtactgccga cccgtttccc cacactgctc gtcaacggct cgtccggtat cgccgtcggt
    18361 atggcgacca acatcccgcc gcacaacctc accgacacca tcaacgcctg tctgcgtctt
    18421 ttggacgaac ccaaaaccga aatcgacgaa ctgatcgaca ttatccaagc ccccgacttc
    18481 ccgaccgggg caaccatcta cggcttgggc ggcgtgcgcg aaggctataa aacaggccgc
    18541 ggccgcgttg ttatgcgcgg taagacccat atcgaaccca taggcaaaaa cggcgaacgc
    18601 gaagccatcg ttatcgacga aatcccctat caggtcaaca aagccaagtt ggtcgagaaa
    18661 atcggcgatt tggttcggga aaaaacactg gaaggcattt ccgagctccg cgacgaatcc
    18721 gacaaatccg gtatgcgcgt cgttatcgag ctgaaacgca acgaaaatgc cgaagtcgtc
    18781 ttaaaccaac tctacaaact gactccgctg caagacagtt tcggcatcaa tatggtggtt
    18841 ttggtcgacg gacaaccgcg cctgttaaac ctgaaacaga ttctctccga attcctgcgc
    18901 caccgccgcg aagtcgttac ccgacgtacg cttttccggc tgaagaaggc acgccatgaa
    18961 gggcatatcg ccgaaggcaa agccgtcgca ctgtccaata tcgatgaaat catcaagctc
    19021 atcaaagaat cgcccaacgc ggccgaggcc aaagaaaaac tgcttgcgcg cccttggcgc
    19081 agcagcctcg ttgaagaaat gctgacgcgt tccggtctgg atttggaaat gatgcgtccg
    19141 gaaggattgg ctgcaaacat tggtctgaaa aaacaaggtt attacctgag cgagattcag
    19201 gcagatgcta ttttacgcat gagcctgcga aacctgaccg gcctcgatca gaaagaaatt
    19261 atcgaaagct acaaaaacct gatgggtaaa atcatcgact ttgtggatat cctctccaaa
    19321 cccgaacgca ttacccaaat catccgtgac gaactggaag aaatcaaaac caactatggc
    19381 gacgaacgcc gcagcgaaat caacccgttc ggcggcgaca ttgccgatga agacctgatt
    19441 ccgcaacgcg aaatggtcgt gaccctgacc cacggcggct atataaaaac ccagccgacc
    19501 accgactatc aggctcagcg tcgcggcggg cgcggcaaac aggcggctgc caccaaagac
    19561 gaagacttta tcgaaaccct gtttgttgcc aacacgcatg actatttgat gtgttttacc
    19621 aacctcggca agtgccactg gattaaggtt tacaaactgc ccgaaggcgg acgcaacagc
    19681 cgcggccgtc cgattaacaa cgtcatccag ctggaagaag gcgaaaaagt cagcgcgatt
    19741 ctggcagtac gcgagtttcc cgaagaccaa tacgtcttct tcgccaccgc gcagggaatg
    19801 gtgaaaaaag tccaactttc cgcctttaaa aacgtccgcg cccaaggcat taaagccatc
    19861 gcactcaaag aaggcgacta cctcgtcggc gctgcgcaaa caggcggtgc ggacgacatt
    19921 atgttgttct ccaacttggg caaagccatc cgcttcaacg aatactggga aaaatccggc
    19981 aacgacgaag cggaagatgc cgacatcgaa accgagattt cagacgacct cgaagacgaa
    20041 accgccgaca acgaaaacac cctgccaagc ggcaaaaacg gcgtgcgtcc gtccggtcgc
    20101 ggcagcggcg gtttgcgcgg tatgcgcctg cctgccgacg gcaaaatcgt cagcctgatt
    20161 accttcgccc ctgaaaccga agaaagcggt ttgcaagttt taaccgccac cgccaacgga
    20221 tacggaaaac gcaccccgat tgccgattac agccgcaaaa acaaaggcgg gcaaggcagt
    20281 attgccatta acaccggcga gcgcaacggc gatttggtcg ccgcaacctt ggtcggcgaa
    20341 accgacgatt tgatgctgat taccagcggc ggcgtgctta tccgtaccaa agtcgaacaa
    20401 atccgcgaaa ccggccgcgc cgcagcaggc gtgaaactga ttaacttgga cgaaggcgaa
    20461 accttggtat cgctggaacg tgttgccgaa gacgaatccg aactctccgg cgcttctgta
    20521 atttccaatg taaccgaacc ggaagccgag aactgaaaat catctcccga tgccgtctga
    20581 agattcagac ggcatttatt tttccctcat ccgtcatcca gcttctcaca atatagcgga
    20641 ttaacaaaaa tcaggataag gcgacgaagc cgcagacagc acaaatatag tggattaaat
    20701 ttaaactagt acagcgttgc ctcgccttgc cgtactatag tcaattaaaa acaag
//
LOCUS       DAXDSD010000030        20694 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXDSD010000030 DAXDSD010000000
VERSION     DAXDSD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20694
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.030"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(102..869)
                     /locus_tag="ACFB2Q_000859"
     CDS             complement(102..869)
                     /locus_tag="ACFB2Q_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC0917912.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            complement(957..1784)
                     /gene="mutM"
                     /locus_tag="ACFB2Q_000860"
     CDS             complement(957..1784)
                     /gene="mutM"
                     /locus_tag="ACFB2Q_000860"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC0917913.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            complement(1837..2502)
                     /locus_tag="ACFB2Q_000861"
     CDS             complement(1837..2502)
                     /locus_tag="ACFB2Q_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFC0917914.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKALAAGAAR
                     VAD"
     gene            2681..4657
                     /locus_tag="ACFB2Q_000862"
     CDS             2681..4657
                     /locus_tag="ACFB2Q_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC0917915.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILD
                     LPPTKQYFQSGSLWDELRQGFRMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKNKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVSIARIQPAAAQTADITVAPLPQETVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQVVPPNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAETSPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4725..5417
                     /locus_tag="ACFB2Q_000863"
     CDS             4725..5417
                     /locus_tag="ACFB2Q_000863"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC0917916.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            5891..7261
                     /locus_tag="ACFB2Q_000864"
     CDS             5891..7261
                     /locus_tag="ACFB2Q_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC0917917.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVAVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITTIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            7494..8150
                     /gene="cmk"
                     /locus_tag="ACFB2Q_000865"
     CDS             7494..8150
                     /gene="cmk"
                     /locus_tag="ACFB2Q_000865"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC0917918.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFTFERILSDIETRDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            8303..9988
                     /gene="rpsA"
                     /locus_tag="ACFB2Q_000866"
     CDS             8303..9988
                     /gene="rpsA"
                     /locus_tag="ACFB2Q_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC0917919.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            9999..10295
                     /locus_tag="ACFB2Q_000867"
     CDS             9999..10295
                     /locus_tag="ACFB2Q_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC0917920.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLA"
     gene            complement(10509..10916)
                     /locus_tag="ACFB2Q_000868"
     CDS             complement(10509..10916)
                     /locus_tag="ACFB2Q_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFC0917921.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            11066..12176
                     /locus_tag="ACFB2Q_000869"
                     /pseudo
     CDS             11066..12176
                     /locus_tag="ACFB2Q_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            12185..13012
                     /gene="fghA"
                     /locus_tag="ACFB2Q_000870"
     CDS             12185..13012
                     /gene="fghA"
                     /locus_tag="ACFB2Q_000870"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC0917922.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNRENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            13542..14693
                     /gene="zapE"
                     /locus_tag="ACFB2Q_000871"
     CDS             13542..14693
                     /gene="zapE"
                     /locus_tag="ACFB2Q_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC0917923.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            14752..15177
                     /gene="ndk"
                     /locus_tag="ACFB2Q_000872"
     CDS             14752..15177
                     /gene="ndk"
                     /locus_tag="ACFB2Q_000872"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC0917924.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            15321..16415
                     /gene="rlmN"
                     /locus_tag="ACFB2Q_000873"
     CDS             15321..16415
                     /gene="rlmN"
                     /locus_tag="ACFB2Q_000873"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC0917925.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            16418..17179
                     /gene="pilW"
                     /locus_tag="ACFB2Q_000874"
     CDS             16418..17179
                     /gene="pilW"
                     /locus_tag="ACFB2Q_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC0917926.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            17195..18460
                     /gene="ispG"
                     /locus_tag="ACFB2Q_000875"
                     /gene_synonym="gcpE"
     CDS             17195..18460
                     /gene="ispG"
                     /locus_tag="ACFB2Q_000875"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC0917927.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKRNKGKVIPIQSL"
     gene            complement(18514..19128)
                     /gene="clpP"
                     /locus_tag="ACFB2Q_000876"
     CDS             complement(18514..19128)
                     /gene="clpP"
                     /locus_tag="ACFB2Q_000876"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC0917928.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(19224..20534)
                     /gene="tig"
                     /locus_tag="ACFB2Q_000877"
     CDS             complement(19224..20534)
                     /gene="tig"
                     /locus_tag="ACFB2Q_000877"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC0917929.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
BASE COUNT         5433 a         5608 c         4770 g         4883 t
ORIGIN      
        1 aacaaactat ccttacggca tttcttttga ggtactcccg ttaccgcagc cttaaggcat
       61 ccgcaaaagc gaatcatacc gctacattcc gacaaccgct ttcagacggc aacatccgca
      121 tcgcctgcga caacggcacg gatgctttct tcgattttat cttttaaagc ataacggtct
      181 tcgctttccg ccgcgtccgc cacgcaaacg aaatcgactt tgatagtcag ttttttcata
      241 gacacgattc gccacaggca agtcggcaaa ccgacatcgg catacgacgg acgggccgtc
      301 ctttttcccg tttcgtcata ataacgcagt gcgaccgcca aaacctttgc ccccgcatcg
      361 atggcggatt ggaacagcgc agccttgaac ggcaggagcc ccagtccgga ggaagttcgc
      421 gcctcaggga aaaaactgac gttttgaccg cgttgcaagg tttcgcagac ggcgcggtta
      481 atcggttcga tgtcgcgccg cgaattgcgg ttgatgaaca ccgttcccgc gttctgcccc
      541 atcttgccca ataccggcca gcttttgatt tcctgcttgg cgataaagct actcggataa
      601 accgcgctca tcgcgaaaat atccagccag gacacgtggt tggcggcaac caagacaccg
      661 ttcggatgtt cgggtgcggg tctgcccacc tccaatccga tatccaaagc cgccaaagcg
      721 cccttgccca acgctataac tgcccgattg cgcgacttgg ggcagccgcc gtcaataccg
      781 cgcaggtttt tgccggtttt gaacagccag accgtcaagc ggcacaagcg gcgcagacgt
      841 gtaaaaaatg aagctttatt tgaagacatg ggcaattctt tcccgataat cggtgtttgt
      901 tgttgaaacg ttcggacggc ataccgataa aatgccgtca gaaaccgttt tccgccctat
      961 ttctgacagt tcgtgcaata aaacgtgccg cgctgcccca aagtttcttt cacaaccaaa
     1021 ccgccgcacc ggaggcacgg ctgattgtgc cgcccgtaca ccgtatattc ctgttgaaag
     1081 tagccgcttt tgccgtcgct gtccacaaaa tccctcagcg tactgccgcc cgtttcaatg
     1141 gcgcgctgca acaccgcttt gacggtttca accaaaaccg cgcactcttt cttttttagg
     1201 cggttggcag gacggtgggg cgaaatgccc gccctgaaca gactctcgtt ggcataaata
     1261 ttgcccacgc cgaccacgac ggtattgtcc atcagagcga gtttgaccgc gcgtttctgc
     1321 gccttcagcc ccgcatacag ataatccgtg caaaatgcct ccgacaaagg ctccggcccc
     1381 agtttttcca acagcggatg acgttcttcg attccctcat accaaagtat cgcgccgaac
     1441 tttctcggat cgcggtaacg catgaccgtg ccgtctgaaa acacaatatc gacgtgatcg
     1501 tgtctgtccg gcctgccgat acgtccgtcc gacggcgtaa agatccgcaa gctgcccgac
     1561 atccccaagt gaatcagcaa aatccccgtt tggaaacgga caatcagata tttggcacgc
     1621 ctgccgcagg acaaaacctg ccggccggac aaaatctccc ccaaatcggg attgatctgc
     1681 cagcgcagct tcagttggcg caatatcacg gcttccaccg tttttccttc aatatgcggc
     1741 gcgatgccgc gcaacgtcgt ttccacttcc ggcaattccg gcataacccc tcccgacatt
     1801 tcttctgaca gatgccgtct gaaagacggc cgtcccttaa tccgcaaccc ttgccgcacc
     1861 cgccgcaagg gctttgccgc cccaataccc gtcccacgcg ggcaggggcg ttacgcccgc
     1921 cgcccttttg accaaaacca aaccgtacac tgcggcacat tgcggccacc aacggtcgcc
     1981 cgccttttcc ataaaccgcc aaaagcgtat ttgcccgagc gacgaaacgg gaggcagata
     2041 caccatgaat ttcccaaatt caatatcgaa accggcatcg gcaagctgcc ttttcaactc
     2101 gggcagcggc agacaaaacc gtttttccgg cagacggaca ccgtcaaacc aacggcagaa
     2161 tccccaaagc gaatacggat tgaaacccgt cagcatcaaa cgtccggacg gtttcaatat
     2221 ccggtgcgct tccgacagga tttgcgaagg tacgccgcat tccagcgtat gcggcaaaag
     2281 cagcatatcc gcaaaaacat ccgccaaagc catattctcc gccgacatcg acatatctcg
     2341 cggcacacag acaatgtctt cagacaggct cagccactga ccgcccacct gaaccgcaca
     2401 cattcccgaa aaacggtatg acgccacata ccgattaaag aagtcctgtt ccaattttgc
     2461 aacataccgc cccatcgccg tatcttcaaa ccatgcatcc atattgcgtc cgtttcaaac
     2521 agattgcctg ccgatatttt attccaaaca ggatttctgt caaaaaacac atcggccgcc
     2581 catttccgaa tccgcataaa gttcctgtaa aacttgacgc tttttcagtc aaacagtacc
     2641 atcggacgat aaaatatgtt tattccgccg agtataaatc atgtccaaac tcaaaaccat
     2701 cgccctgacc gcatcaggtc tgtccgtttg tccgggtttc ctatatgccc aaaacacctc
     2761 atcacaccaa gtcggtttag cgattatgcg gttaaactct tcaatactcg acctgccacc
     2821 gacaaaacaa tatttccaat ccggcagcct gtgggacgag ctgcgccaag gcttccggat
     2881 gggcgaagtc aatcccgaac tggtacgccg ccacgaaagc aaattcatcg caagccgcag
     2941 ctatttcgac agggtcgtca accggagccg accctatatg taccatatcg ccaacgaagt
     3001 caaaaaacgc aatatgcccg ccgaagccgc cctgcttccc ttcatcgaaa gcgcgttcgt
     3061 caccaaagcc aaatcacacg tcggcgcatc gggcctgtgg cagttcatgc ccgctaccgg
     3121 caggcattac ggcttggaaa aaacaccggt ttacgacggc aggcacgacg tttacgcagc
     3181 caccgatgcc gcactcaact atctgcaata tctctatgga ctgttcggcg actggccgct
     3241 cgcctttgcc gcctacaact ggggtgaagg caacgtcgga cgcgccgtca accgcgcccg
     3301 cgaccaaggg ctcgaaccga cctacgaaaa cctgcgtatg cccaacgaaa cgcgcaacta
     3361 tgtccccaag ctgctcgccg tgcgcaacat tattgccacc ccccaatctt tcggcatgaa
     3421 tatcagcgac atagacaaca aaccctattt tcaggcagtc gaaccgggcc gtccgctcga
     3481 caacgaagcc atcgcccggc ttgccggcat cacgcaaagc gagctgctcg ccctgaatcc
     3541 tgcattcaac gtccccgcgt tcatccccaa aaacaaacgc aaactgctgc ttcctgtcgc
     3601 gtccgtccaa accttccaaa gcaactacct caacgccgca cccgacagcc tgttttcatg
     3661 ggaagtctat acgcctgccg ccaaaaccag cctgtccgac atctcgacgg caaccggcat
     3721 gagcattgcc gacatcaaac gcctcaacaa cctgaacggc aaccttgtca acgcaggacg
     3781 cagcatcctt gtcgccaaga acggcaagac ccttcatacg gcatcggaat ccgtcgtttc
     3841 catcgacatc gacaatacgc ccgacaccta ccgttccaat atgccggcag gcacggtgaa
     3901 cgtcagcatt gcccgaatcc aacccgccgc cgcacagaca gcggacatta ccgtcgcacc
     3961 tttgccgcaa gaaaccgtcc gtacggaacc cgatcccctt gtccgcattg ccgaacccgc
     4021 ccttgcgaca gccgcagcgc aacctcaaac cgaaaaacag actgccatgc cgtctgaaac
     4081 ccaaaccgca acactcgcgc aggtcgtccc cccaaacgac atgcaggcgg cagacgaact
     4141 catgcagctt gttgcccgaa acaacctgcg ccggcaggct gaagaaacca tctccgccgt
     4201 catcggcacg cctgacacag ttgccgaaca caacatttcc tcatctccgc aacataccgt
     4261 tgccgccgac ggcaaacgcc gggcacgttt ggaaacgcgc gtagccaaag ctgccgacgg
     4321 cgaagccgaa acctccccgc tccatgccag catccaccgc gttgtagaag gcgacaccct
     4381 gttcaacatt gccaaacgct acaacgtcag cgtagccgac ctgattgtcg ccaacaacat
     4441 caaaggcaac accatccaaa aaggacaggt actccgcctg gcgcaggcag cccctgccca
     4501 aacccgtatt gaaaaagtat cctacaccgc gcgcaaaggc gacaccttca aaagtatcgc
     4561 cgcgcgcttc aatatccata tcgacgacat ccgccggctc aatcccaacc tgaacaccat
     4621 caatccggga cagagggtca aactgattgg aagctgattc ggatacggca catgacagga
     4681 ctttctcagt cctgtctttt tcatcccaca tttcccatac catcatgaaa ctcatcaaat
     4741 acctgcaata tcaaggcata ggaagccgca agcagtgcca atggctgatt aggggcggct
     4801 acgtctccat caacggaacc tgcatggacg acaccgatgc agacatcgat tcctcatacg
     4861 tcgaaacgtt ggatattgac ggggaagcag taaccgtcgt tcccgaaccc tatttctaca
     4921 tcctgctcaa caagcctgaa gattacgaaa cttcgcacaa accgaaacac taccgcagcg
     4981 tattcagtct gttccccgac aatatgcgga acatcgatat gcaggcggtc ggcaggctgg
     5041 atgcggatac gaccggcgta ttgctgatta ccaacgacgg caaactgaac cacaacctga
     5101 cttcgccgag cagaaaaatt cccaagctat acgaagtaac gctcaaacac cctacaggag
     5161 aaacgctctg cgaaactttg aaaaacggcg tgccgctcca cgacgaaaac gaaaccgttt
     5221 gtgccgccga tgccgttttg gaaaatccga ccaccctgct gctgaccatt accgaaggaa
     5281 aataccacca agtcaaacgc atggttgccg ccgccggcaa ccgcgtgcaa caccttcacc
     5341 gccggcgatt cgcacatttg gaaacagaaa acctcaaacc cggggaatgg aaatttatcg
     5401 aatgtccgaa attctgaaac aaaattccat aatttcccta tattcaacca tatgcttatt
     5461 aaataagcag ttttacaaaa attaactcaa aaaacagtta gtttacaata taatccgctt
     5521 gtctaagtaa cgcctgttca agcaggatta tgagcaagac gttttccccg taatgtgttt
     5581 tgccatctat acttcccccc ttgtatagat ggttttctat ttcaaattcc ctctctttca
     5641 tcatcattga aatgctatca ttaatatgat taacaaaatt tcacttccgt acaccgatgt
     5701 agaaaaataa cagattaatc tgttcataaa ctccgattct tcctcatcgg tttatactca
     5761 tcagaaatat tcactgaaat atatgcattt tacatggaaa atcactaggc agttgaaaaa
     5821 cattcagtca atttctgtta agaatcacgt gtaaaatttc cgctttatct caatcggaca
     5881 cggtttcaat atgtcttccc cttcaaatac caaccgtcaa acctggtcca gccgtttgac
     5941 ttatatcctt accgttgccg gcgcgactgt cggtttcggc gcgacgtggc gtttcccgta
     6001 tttggtcggt gaaaacggcg gcggcgcgta tgtgttttta ttctgtatcg cgatgctggt
     6061 tatcggcatc ccgatgattt tggtggaaaa cgtcatcgga cggcgcaaag gcgtgaacgc
     6121 gctggatgcg ttcggcggcc cgatgaacgg caaacctgtt gccaaaattt ggaaactggt
     6181 cggccggatg gggctgctcg gcgcgttcgg catcatggct tattatatgg tactcggtgg
     6241 ctgggtaatc agctatatcg ttaatattat tggaggaaat ttgaatattt ccagccccgt
     6301 cgacggtgtg gttacaaaag gcttctttac cgaacacatt gaaaacagcc cttgggaaat
     6361 tgcgttttat acgctgcttt ttgtcgccgt gaaccaatgg attttggtca aaggcgttat
     6421 cggcggcatt gaaaaaacgg cgaaatacct gatgcctctg ctgtttttgt tcctaatcgc
     6481 gatggtcgtc cgcaacgtta cccttccggg cgcaatggaa ggggttgctt tctatctgaa
     6541 acctgatttc agcaagatta ccgccgaact gttcgtcttc gttttgggac aggtattttt
     6601 tgccctgagc ctgggtttcg gcgtgatgat taccttatcc agctatctgg ataaaaacga
     6661 aaatctggtt cagacggcag ttatcacggc aattaccaat accatcatcg ctgtacttgc
     6721 gggctttatg attttcccgt cgctcttcag cttcggcgtc gcgcccaatt ccggcccgac
     6781 tttggtgttc caaagtttac cgattgtgtt ctcacatatg tgggcaggac ctgtgttcgc
     6841 cgtcattttc ttctctctgc tcctgattgc cgcgttgaca acttcgctga cgatttatga
     6901 agtgttgatt acgaccattc aggaaaaaac caaaatccgc cgtaccgccg caatcacgat
     6961 tgtattgtcc gtcatcttcg ttttcggcaa catcccgtct attttgagct atggcccgtg
     7021 gaaagacgtt tccgtgttcg gcaaaaatat tttcgatgcc ttcgactaca tcagcggcaa
     7081 catcttgttt atgctgaccg cgctcggttc cgcattgttt gtcggttttg tgatgaagga
     7141 cgaagcgaag gaagaattgc tttataaagg caaccatacg acggtcaata tttggtttgc
     7201 ctatgtgaaa taccttgtgc cgctggtgat tctgctgatt ttcgtcagca acctgttcta
     7261 atccgcagca atcaatgccg tctgaaggtc atacctcttt cagacggcat tgcattgatg
     7321 cccgccacgc cggaatccga accggcttgc cggcttctgt cggacgcttt ccgggcaggc
     7381 ggcgttccgt ctgcttagac aatcgtcctt taaaacaggt agaatccgcc ccaacgggaa
     7441 acacaccctt cagacggcaa aacccatacc ccaaaccatc aggaatcccc cttatgaaca
     7501 gacaaaaagt catcgccatc gacggtccgg gcgcatcggg caaaggcacg gtcgccgccc
     7561 gcgttgccgc cgcattggga tacgattatc tcgataccgg cgcgctctac cgcctgaccg
     7621 ccctatatgc acaaaaacaa ggcgtggaat ggcacgatga agaaaacgtt tccgcacttg
     7681 ccaaaaaact gcccgccgta ttttcaggca accgcatcct acttgatggc gaagacgttt
     7741 cagacggcat ccggacagaa gccatcggta tgggcgcatc cgcagttgcg caatggccca
     7801 aagtccgcgc cgcactgctg caacgccaac gcgattttct gaccgaaaaa ggactggttg
     7861 ccgacggacg ggacaccgga tcggtcgtct tcccccaagc cgaactcaaa atttttctga
     7921 cagccgaatc taaaatccgt gccgaacgcc gcgccaaaca aatcggcatt ccctgcgaag
     7981 gtttcacatt cgagcgcatc ctgtccgaca tcgagaccag agacgaagca gaccgaaacc
     8041 gcaaggttgc ccccctgaaa caacagcccg atgccctgct tttggatacg agccgcctaa
     8101 ctatagaaga aactgtaaaa aaagtgcttg attggtatcg taaagtttaa attttcaggt
     8161 ataatcgccc gcattgcgct tcagacagca tgaatttcca tgtgccgtct gaaaccttat
     8221 ttacccgcct gccgtgccaa ggactgcaga cacccaccaa cccaacccgc accccttggc
     8281 ggtgtaccga aaagagttat atatgtctat ggaaaatttt gctcagctgt tggaagaaag
     8341 ctttaccctg caagaaatga acccgggtga ggtgattacc gctgaagtag tggcaatcga
     8401 ccaaaacttc gttaccgtaa acgcaggtct gaaatcagaa tccctgatcg atgtagctga
     8461 attcaaaaac gctcaaggcg aaattgaagt taaagtcggc gacttcgtta ccgttaccat
     8521 cgaatccgtc gaaaacggct tcggcgaaac caaactgtcc cgcgaaaaag ccaaacgcgc
     8581 agccgattgg atcgctttgg aagaagccat ggaaaacggc aacatcctgt ccggcatcat
     8641 caacggtaaa gtcaaaggcg gcctgaccgt tatgatcagc agcatccgcg cattcctgcc
     8701 gggttctttg gtcgacgtac gtcccgttaa agacacttcc cattttgaag gcaaagagat
     8761 cgaattcaaa gtgatcaaac tggacaaaaa acgcaacaac gtcgttgttt cccgccgcgc
     8821 cgttttggaa gccactttgg gtgaagaacg caaagccctg ctggaaaacc tgcaagaagg
     8881 ctccgtcatc aaaggcatcg tcaaaaatat caccgactac ggcgcattcg ttgacctggg
     8941 cggcatcgac ggcctgctgc acatcaccga tttggcatgg cgtcgcgtga aacacccgag
     9001 cgaagtcttg gaagtcggtc aggaagttga agccaaagta ttgaaattcg accaagaaaa
     9061 acaacgtgtt tccttgggta tgaaacaact gggcgaagat ccttggagcg gtctgacccg
     9121 ccgttatccg caagccaccc gcctgttcgg caaagtatcc aacctgaccg actacggcgc
     9181 attcgtcgaa atcgaacaag gcatcgaagg tttggtacac gtctccgaaa tggactggac
     9241 caacaaaaac gtacacccga gcaaagtcgt acaactgggt gacgaagtcg aagtcatgat
     9301 tttggaaatc gacgaaggcc gccgccgtat ctctttgggt atgaaacaat gccaagccaa
     9361 tccttgggaa gaatttgccg ccaaccacaa caaaggagac aaaatctccg gtgcggttaa
     9421 atccattacc gatttcggcg tattcgtcgg cctgcccggc ggcatcgacg gtctggttca
     9481 cctgtccgac ctgtcttgga ccgaatccgg cgaagaagcc gtacgcaaat acaaaaaagg
     9541 agaagaagtc gaagccgtcg tattggcaat cgatgtggaa aaagaacgca tctccttggg
     9601 tatcaaacaa ctggaaggcg atcctttcgg caacttcatc agcgtgaacg acaaaggttc
     9661 tttggttaaa ggttccgtga aatctgttga tgccaaaggc gctgttatcg ccctgtctga
     9721 cgaagtagaa ggctacctgc ctgcttccga atttgcagcc gaccgcgttg aagacttgac
     9781 caccaaactg aaagaaggcg acgaagttga agccgtcatc gttaccgttg accgcaaaaa
     9841 ccgcagcatc aaactttccg ttaaagccaa agatgccaaa gaaagccgcg aagcactgaa
     9901 ctccgtcaat gccgccgcca atgcgaatgc cggtaccacc agcttgggcg acctgctgaa
     9961 agccaaactc tccggcgaac aagaataagg ttgcagacat gacaaagtct gagttaatgg
    10021 ttcgcttggc agaagtattt gccgccaaaa acggcacgca tcttctggca aaagacgtag
    10081 agtacagcgt aaaagtcttg gttgacacca tgacccgatc gcttgcccga ggtcaacgca
    10141 tcgaaatccg cggtttcggc agcttcgatt tgaaccatcg tcctgcccgc atcggtcgca
    10201 atcccaaaac cggcgagcgc gtggaagtac ctgaaaaaca tgtaccccac ttcaagcctg
    10261 gtaaagaatt gcgcgagcgg gtcgacttgg cttgaaaaga aaatgccaat taaaccttag
    10321 catcaaaacg ccgctggtac gcggcgtttt ttctttggtt taacttcatc cgctgcttca
    10381 ataccttaag ccaagcaagc aacggattag agcgtggatt ttttaccatt gcttacaatc
    10441 cgctttttaa acaacaaatt gttgatttct attacgaaca ggacaaaatc ctgcttattg
    10501 cactaaaact aagccgtttc aggaatttgc ggcaaattta cagcttttac cgagcctaat
    10561 gctttcgctt tttggtaaaa cgccaatttg tattcaagca aatctaaata acgttttaat
    10621 tcggcaattt gacacttcac attttctatt tgattttcaa acaaggaaag gcgttcttca
    10681 atggtatcgt cgccaatgac ggtacattcc gcaaagcgtt tgatgtcttt taagctcatt
    10741 cccgtatttt tcaagcattg caataagtac aaccattgca aatcgttatc ggtaaaacaa
    10801 cggttaccgt attcatcacg tccgatatta ggcaacaagc cttctttgtc gtaaaagcgt
    10861 aaagtgtggg cggagatgcc tattttttcg gcagctttgg cagtggtata agtcattttc
    10921 cctccctcta aacaaaaaac ggtaaaaaaa cacttgcctt agagtgaact ctaaaatgta
    10981 aactgttgct atgttgctca ggcaaggcta ttttgttaat gaattaagag gaaagacaat
    11041 ggaaatgaaa caaaccgatt caaccatcaa atctcgtgcg gcggtggcat tcgcccctaa
    11101 ccaaccctta caaattgtgg aaatcgacgt ggaaatgccg cgtaaagacg aggtgttaat
    11161 tcgtaacacc cacactggcg tgtgccatac tgatgcattt accttatcag gaagcgatcc
    11221 tgaaggcgta ttccctgtgg tgcttggaca cgaaggtgcg ggtgtggtcg ttgctgtggg
    11281 cgagggtgtg tcaagcgtaa aaccgggtaa tcacgtcatt ccgctttaca ccgcagaatg
    11341 tggcaaatgt gagttttgcc tctcgggtaa aaccaattta tgcgtctcag tgcgtgatac
    11401 acaaggtaaa ggcttaatgc cagacggcac gacgcgtttt tcttatcaag gtcagccgat
    11461 ttatcactat atgggctgtt cgactttcag tgaatattct gttgttgccg aagtttcact
    11521 ggcgaaaatc aacccgaaag ccaaccacga acaagtatgt ttgctcggct gcggcgttac
    11581 cactggtatt ggtgcggtac ataacacggc aaaagtacaa gaaggcgatt ctgttgccgt
    11641 gtttggcttg ggagcgattg gtttggctgt ggtgcaaggt gcgcgtcaag ccaaagccgg
    11701 tcgcattatc gccattgata ccaatcctgc aaaattcgag ttggcaaaac agtgttggtg
    11761 caactgactg tttgagcccg aaagattacg ataaaccaat caaagatgtg ttgttagaca
    11821 tcaacaaatg gggcattgac cacacctttg aatgtatcgg caatgtaaac gtaatgcgtc
    11881 aggcattaga aagtgcacac cgaggttggg gacaatccat catcatcggc gtagcggatg
    11941 caggacaaga aatttcaacg cgtccgttcc aatcggtaac aggccgtgtt tggaaaggct
    12001 cggcatttgg tggtgttaaa ggccgctccg agcttccgaa aatggtggag gattcaatga
    12061 aaggcgatat ccaattagaa ccatttgtga cccatgcaat gacccttgat caaatcaatg
    12121 aagcctttga gttaatgcac gaaggtaaat cgatccgcgc tgttattcat tactaaggtc
    12181 cacgatgaaa ctgattgaac aacatcaaat ttttggtggt tcgcaacaag tttgggcgca
    12241 tcatgcccaa acgcttcaat gcgaaatgaa atttgccgtc tatttgccgg ataatcggga
    12301 aaatcaaccg cttggtgtga tttattggct ttccggcttg acgtgtacgg aacaaaattt
    12361 cattaccaaa tcgagcttcc ggcgttatgc ggcagaacat caagtggttg tggtcgcccc
    12421 cgataccggc cctcgcggag agcaagtgcc gaacggtgcc gcttatgatt taggacaggg
    12481 ggcaggcttt tatttgaatg cgaccgaaca gccttgggcg gcgaattatc aaatgtatga
    12541 ttacattttg aacgagttgc cccgtctgat tgaggagcat tttcctacca acggcaaacg
    12601 ttccattatg gggcattcga tggacggaca cggcgcattg gtattggcac tacggaatcg
    12661 ggaacattat caaagtgttt ctgccttttc gcctatttta tcgccaagcc tcgtaccgtg
    12721 gggagaaaaa gcctttactg cttatttagg gaaagaccgt gaaaaatggc agcaatatga
    12781 tgctaactca ctcattcaac aaggctataa agtgcaaggt atgcgtatcg atcagggctt
    12841 ggaagatgag tttttgccga cacaattgcg taccaaagat tttatcgaaa cctgtcgtgc
    12901 ggcaaatcag ccaatcgatg tgcgtttcca taaaggatac gatcacagct attacttcat
    12961 cgccagtttt attggtgagc atatcgctta tcacgccgca tttttgaagt aagtcaaaga
    13021 gcggtcagtt ttcaaagcag tttggaatag ccggcacgag ggcggtaaga agtgccggca
    13081 taaacgtatg ccgcctgaac cagaaggagc cggccctacg gattaccaaa ccctgccctt
    13141 gatttgatta gtatgatttt taagcttaaa ccctgattga tcaaacatta aaatcgcccg
    13201 tatttcctac ttcactttcg ggcttcgtat tattcgttgc cgaatttgca taagcaacct
    13261 gttcctgttg tgttttcgcc ccctccaagc tgacggtggc gtaacagctt cgcgattcac
    13321 catctgtttc atttgtcctc cttcaatatt aaaacgcagc ctgaaataaa aggcattttg
    13381 gctcatatct gcaccatatt aaaacgccgc ctttgcttat accccctttg tgcgcgtcat
    13441 tattcttttc cacgggaaac gccaagtttg aaggaaatca tttataatac cagcggtaag
    13501 cattttcttt cttagccgca agaagtataa caaggttaaa tatgaataat agagaccaac
    13561 tttttaaagc cccgccgttt gaaaaccaca gcccgctgac ctggtatcag gctgcctcac
    13621 aactgcccaa cttcatccgc gacgacgcac aggcggccgc catcgaacac cttgaccggc
    13681 tttggaccga attgatgatg ttcaaacgca aaagaaaccg ttttttaggc aggagtttgc
    13741 gttccccgca agttcccaaa gggctttatt tctatggcgg agtcggacgc ggcaagagct
    13801 ttctgatgga cgcttttttc ggctgcctcc cttaccgccg caaacgccgc gtccactttc
    13861 atgcctttat ggcggaaatc caccggcggc tgaaagccct gaaaagcgaa agcaacccgt
    13921 tgaaatccgt tgccgccgag attaccaaag aaacccgagt attgtgtttt gacgaatttc
    13981 atgtcagcga tattgcggat gcaatgattt taggccgtct gctggaaaac ctgcttaacg
    14041 agggcgttgt tttggtggcg acttcaaact acgcgccttc cgaactctac ccgcaaggtc
    14101 aaaaccggag cggttttctt cccacaatcg cgctcatcga gtccagtctg accgtcttaa
    14161 acgttgacgg aggggaagac taccggctgc gtaccctccg ccccgccgag attttcttta
    14221 cgcctgccaa tgaagaaaat gaggcaaaac tggcaaaact gttcaaagaa atgacaggca
    14281 ttaccgattt gaaccccggc atcagcacca tccacggccg ggaaattccc cacaaagccg
    14341 agtccggtcg taccatatgg tttgatttcc gcgcactgtg cttcagtccc cgctcacagt
    14401 ccgactatct gtatttggcc gaacattatg aaatggtttt tatttcaggt ttggaacaac
    14461 tctcaccgca agaaaaggcg gaggcgcgac ggctgacttg gctgattgac gtactctacg
    14521 atttccgggt caaactgtgt gccaccggcg cggtagatgt caaccatatc tacacggaag
    14581 gcgattttgc cgaagaattt acccgcaccg ccagccggat ggtcgaaatg cagtccgaag
    14641 tttatttgga acagccgcac ctgaccttat ctcccaaggc ttcaggcgga taagttattt
    14701 ttttggtaga ataccgattt gattcttctt aagtaaaaat aaggatatag catggcgatt
    14761 gaacgtacca tatccatcat caaacccgat gccgtcggca aaaacgttat cggcaaaata
    14821 tacagccgct ttgaggagaa cggtctgaaa atcgttgccg ccaaaatgaa gcagcttacc
    14881 ctcaaagaag cccaagaatt ttatgcggtt cataaagacc gccccttcta tgccggattg
    14941 gttgaattta tgaccggcgg tccggttatg attcaagtac tggaaggcga aaacgccgtt
    15001 ctgaaaaacc gcgaactgat gggggcgacc aatcccaccg aagccgcaga aggcacgata
    15061 cgcgcggact ttgccacttc ggtcagcatt aacgccgtac acggttccga cagtgtggaa
    15121 aatgccgctt tggaaattgc ctactttttc agccaaaccg aaatctgccc ccgttgatac
    15181 aatacaccgc ccaattccta ttcagacggc ataaaatata tccatgccgt ctgaaaactc
    15241 tgttgcaaaa gacttcaaat caaacttgcc tgccctgcaa ttttttattt gaagccttaa
    15301 tttaagaaaa acacaaatac atgaaaacca atctgctcaa ctacgacctt caagggctaa
    15361 cccgacattt tgccgatatg ggtgaaaaac cattccgcgc caaacaggtt atgcgttgga
    15421 tgcaccaatc cggcgcgcaa aattttgacg aaatgaccga tttggcaaaa tcgttgcgcc
    15481 ataaactgaa cgaacaggca agcatcgaaa ttcccaagct gatgatgtcc caagaatctt
    15541 cagacggcac tcgaaaatgg cttttggatg tcggtacggg gaacggcgtg gaaaccgtct
    15601 tcatccccga atcggatcgc ggcacgctct gcatttcctc gcaagtcggc tgcgctttgg
    15661 aatgtacatt ttgttcgacc ggccggcagg gcttcaaccg caatttaacc gctgccgaaa
    15721 tcatcggaca gttgtggtgg gcaaacaaag cgatgggcgt tacaccgaaa aacgagcgtg
    15781 tgatttccaa cgtcgtcatg atgggcatgg gcgagccgat ggcgaacttc gacaatgtcg
    15841 ttaccgcctt gagcatcatg ctggacgacc acggctacgg tttgagccgc cgccgcgtaa
    15901 ccgtttccac ttcgggtatg gttccccaaa tggacaggtt gcgcgatgtc atgccggtgg
    15961 ccttggcggt ttccctccac gcttccaatg acgaagtccg caaccaaatc gtaccgttga
    16021 acaaaaaata tcccttgaaa gaattgatgg ccgcatgcca acgctatctg gtcaaagcgc
    16081 ccagggattt catcactttc gagtatgtga tgctggacgg tgtcaacgat aaggcacaac
    16141 atgcatacga acttattgaa ctggtcaaag atgttccctg caaattcaat ttaatcccgt
    16201 ttaatccttt ccctaattcg ggatatgaac gttcaagtaa cgaaaacatc cgtattttca
    16261 gagatatcct gcaacaagcc gaatttgtcg ttaccgttcg aaaaactcgt ggcgacgata
    16321 tcgatgccgc ctgcggacag ttggcaggtc aggttcagga taaaacgcgc cgccaacaaa
    16381 aatggcagca gattttaatc ggacaacagg ggtaattatg ccttttaagc catccaaacg
    16441 aatctcttta ttacttgttc ttgccttggg cgcgtgcagc acttcctacc gcccctcgcg
    16501 ggcagaaaaa gccaatcagg tttccaatat caaaacccag ttggcgatgg aatatatgcg
    16561 cggtcaggac taccgtcagg caacggcaag tattgaagat gccttgaaat cgaaccctaa
    16621 aaacgagctt gcctggctgg tccgtgccga aatctatcaa tacctgaaag ttaacgacaa
    16681 ggcgcaggaa agtttccggc aagccctctc catcaaaccc gacagtgccg aaatcaacaa
    16741 caactacggc tggttcctgt gcggcaggct caaccgccct gccgaatcta tggcatattt
    16801 cgacaaagcc ctggccgacc ccacctaccc gaccccttat attgccaacc tgaataaagg
    16861 tatatgcagc gcaaaacagg ggcaattcgg attggcggaa gcctatttga aacgttccct
    16921 cgccgcccag ccgcagttcc cacccgcatt taaagaactg gcgcgcacca aaatgctggc
    16981 cgggcagttg ggcgatgccg attactactt taaaaaatac caaagcaggg tagaagtcct
    17041 tcaggccgat gatttgctgc taggctggaa aattgccaaa gccctcggca acgtgcaggc
    17101 ggcatacgaa tatgaagcac aattgcaggc aaatttcccc tactcggaag aattgcaaac
    17161 cgtcctcacc ggtcaataaa cagattcaaa ctatatgaac acactccaac gccgcaagac
    17221 gcatcaagtc ctcatcgatc atattaccgt cggttcagaa gcacccgtcg ttatccaatc
    17281 tatgaccaac accgacactg ccgatgcaaa agccaccgca ttgcagatta aggaattgag
    17341 cgatgccggc tcggaaatgg tgcggattac cgtcaacagc cccgaagccg cgtccaaagt
    17401 tgccgaaatc cgccgccgct tggacgatat gggctatgcc acaccactga ttggcgattt
    17461 ccattttaac ggcgaacgcc tgttggcgga atttcccgaa tgcggcaaag cattgtccaa
    17521 ataccgcatc aatcccggca atgtcggcaa aggcgtaaaa ggcgatgaaa aatttgcctt
    17581 tatgattcgg actgctgctg aaaacgataa agccgtccgt atcggcgtaa actggggttc
    17641 tttggatcag agcctcgcca aacgtatgat ggatgccaac ctcgtttctt ccgcgccgaa
    17701 accgcccgaa gaagtgatga aggaagcact gattgtctcc gctttggaat ctgccgaaaa
    17761 agccgttcta ttgggactgc ccgaagacaa aatcatcctg tcgtgcaaag tcagcgcggt
    17821 tcatgatttg attcaggttt accgcgaact gggcagccgt tgcgtctatc cgctgcattt
    17881 gggtttgacc gaagccggta tgggcagcaa aggcattgtc gcatcaacgg cggcattatc
    17941 tgttttgctt caagaaggaa ttggcgacac catccgcatt tcactcactc cggaaccggg
    18001 cagcccgcgt actcaggagg tcgtcgtcgg gcaagagatt ttacagacta tgggtttgcg
    18061 ctcgtttacg ccgatggtta ccgcctgccc gggatgcggg cgtaccacca gtaccgtatt
    18121 tcaagagctg gcacaagatg ttcaaaatta cctgcgccaa aaaatgtcta tatggcgtac
    18181 cctttatccc ggggttgaat ccctgaacgt tgccgtaatg ggctgcgttg tcaatggccc
    18241 cggagaaagc aaattggccg acatcggaat cagccttccc ggtacggggg aaacacccgt
    18301 cgcacctgtt tatgtagatg gggagcgcaa agtcacactg aaaggtaata atattgcaag
    18361 cgaattccta gctattgtag aggaatatgt taaaaccaat tatggcaaaa acagttctaa
    18421 acgcaataaa ggaaaagtca tcccgataca gtctctataa aacaaatgcc gtctgaacgt
    18481 ttcagacggc atttgtttct ctcagatttc ttattaaagc cgcaaagaag cgcggttttc
    18541 caaaacttgg tcgatcaaac catattcttt tgcttcttcg gcagacatga aattatcacg
    18601 gtcggtgtcg cgctccaaat ctgccaaatc gcggccgcaa tgtttcgcca tcaggcggtt
    18661 gagtttttct ttgattttca acaactcgcg tgcgtggatt tcaatgtcgg atgcctgacc
    18721 gcccaagccg ccgctgatta aaggctggtg aatcataatc cggctgttgg gcagggcgaa
    18781 acgtttgcct ttctcgcctg ccgacaataa gaacgcgccc atacttgccg cctgccccaa
    18841 gcacaaagtc gatacatcgg gcttgatgaa attcatcgtg tcgtaaatcg acataccggc
    18901 cgttaccgag ccgccggggg aattgatgta gaagaaaata tccttatccg gattctcact
    18961 ttccaaaaac aacagttggg cgaccaccag attagcagac tcatcggtta ccgggccaac
    19021 cagaaatacg atgcgctctt tcaaaagccg ggaatagata tcgaatgcac gctcaccgcg
    19081 accgctctgc tcgataacgg tagggacaag atggttatca aaagacattt cgtctccttt
    19141 catgatggaa aagcaccaaa gcgggcttta aaaacggctt cggtgctttc aaaaactgcc
    19201 ttcagacagc attttcagaa caatcaggct tgcgcgccca tcacttcgtc aaaagacaaa
    19261 gcttttttgt ttactttggc tttgcccaaa acgaaatcaa caacgttgct ttctactgcc
    19321 aaagaagtcg gggcttgcag gcgggaagta tctgcgtagt accagtcaat cacttcttga
    19381 ggatcttcgt agctttctgc gaagttggcg acaacggctt tgatttgctc ttcagtcggt
    19441 tccagtttgt tttcgtcaac cagtttggcc aaaatcagac ccaaagatac gcgacgttcg
    19501 gcttgttctt tgaacatatc caaaggcaaa tccaagttcg cggcatcggt cataccttgg
    19561 ttaacgaagt tttgtttcat ttcgtttgcc aggcgggcgg cttcttcatt gaccaaagca
    19621 accggaactt tcaactctac ggctttaatc agcgcgttca ttacggattc tttggtttgt
    19681 tcgttcacgc ggcgttccac ttcgcggctt acgtttttct tcacttcttc acgcattttg
    19741 gcaacatcgc catccgcaat acccaaggct tttgcaaaat ctgcatcgac ttcaggcaga
    19801 gtgggctcgg aaacattgtt cagcgtaatg gtgaacacgg cagttttacc ggcaacatct
    19861 ttgccgtggt attcttcagg gaagttgacg gtaacgtctt tactttcgcc cgctttcatg
    19921 ccgactacgc cggcttcaaa ttcaggcagc atttgacctg cgcccaatac gaaggcgtag
    19981 tttttggatg cgccgccggc aaaaggttcg ccgtcgattt tgccttcaaa gtcgatgatg
    20041 acgcggtcgc cgtttcgggc ttcgcggtca acatggttga agcgggtacg ttgtttacgc
    20101 aggatttcta cagtttggtc gacttcggca tcgccgacgg aagcggttac tttttcgacc
    20161 tcttgtgcag acaaatcgcc gataacgact tcggggaaca cttcaaaaat ggcggcaact
    20221 ttgaaagact ctttatcgtc ttgttcttca acgccttcaa aacgtggata gcctgccact
    20281 ttcaactctt gggcaacggc aacatcgtag aagcggcgtt gcaccagctc gttgatcacg
    20341 tcgttttgcg cgctcgcacc gtacatttgg gcaatcattt ttaaaggtgc tttacccgga
    20401 cggaaaccgt cgatttttgc acggcgttgg gtttgtttca gttttttatc ggtttctgcg
    20461 ttgatttcgg accaaggcag ggacaacact actttgcgtt ccagattttc taaaatttca
    20521 acagttacgc tcatcataag cccttaaatt tgttgtgttg ataaaatgat aaaccttctt
    20581 ccctacatgg ggaagcaaac agcgcaacgg tacgatattt gaaccgcatt gccgcaaagg
    20641 ggaaatttta gctggcaagt atatcacaat gtttcgcctg aaacataata tgcc
//
LOCUS       DAXDSD010000031        20237 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXDSD010000031 DAXDSD010000000
VERSION     DAXDSD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20237
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.031"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            19..665
                     /locus_tag="ACFB2Q_000878"
                     /pseudo
     CDS             19..665
                     /locus_tag="ACFB2Q_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(737..2482)
                     /gene="recD"
                     /locus_tag="ACFB2Q_000879"
     CDS             complement(737..2482)
                     /gene="recD"
                     /locus_tag="ACFB2Q_000879"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC0917930.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALGSMLERVFSQE"
     gene            complement(2549..3244)
                     /gene="lolD"
                     /locus_tag="ACFB2Q_000880"
     CDS             complement(2549..3244)
                     /gene="lolD"
                     /locus_tag="ACFB2Q_000880"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC0917931.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            complement(3237..4484)
                     /locus_tag="ACFB2Q_000881"
     CDS             complement(3237..4484)
                     /locus_tag="ACFB2Q_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC0917932.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            4710..4988
                     /locus_tag="ACFB2Q_000882"
     CDS             4710..4988
                     /locus_tag="ACFB2Q_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917933.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            complement(5044..5661)
                     /gene="recR"
                     /locus_tag="ACFB2Q_000883"
     CDS             complement(5044..5661)
                     /gene="recR"
                     /locus_tag="ACFB2Q_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC0917934.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(5728..7266)
                     /locus_tag="ACFB2Q_000884"
     CDS             complement(5728..7266)
                     /locus_tag="ACFB2Q_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC0917935.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(7315..7689)
                     /locus_tag="ACFB2Q_000885"
     CDS             complement(7315..7689)
                     /locus_tag="ACFB2Q_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC0917936.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            7839..9467
                     /locus_tag="ACFB2Q_000886"
     CDS             7839..9467
                     /locus_tag="ACFB2Q_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC0917937.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALDKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            9546..10778
                     /locus_tag="ACFB2Q_000887"
     CDS             9546..10778
                     /locus_tag="ACFB2Q_000887"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC0917938.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYGFEIAEETIKLMRQM
                     VENGEADALVAERVWQELAKGLMEKNPRKMIEMLRECGALQVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDINGVE
                     PVREVNQRLRAPRQCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHFIAEQI
                     DRARLAQIAPLQKAFRGA"
     gene            11197..11505
                     /locus_tag="ACFB2Q_000888"
     CDS             11197..11505
                     /locus_tag="ACFB2Q_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917939.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            11587..12618
                     /gene="ruvB"
                     /locus_tag="ACFB2Q_000889"
     CDS             11587..12618
                     /gene="ruvB"
                     /locus_tag="ACFB2Q_000889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC0917940.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMGEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(12863..13546)
                     /gene="rpe"
                     /locus_tag="ACFB2Q_000890"
     CDS             complement(12863..13546)
                     /gene="rpe"
                     /locus_tag="ACFB2Q_000890"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC0917941.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            13692..14126
                     /locus_tag="ACFB2Q_000891"
     CDS             13692..14126
                     /locus_tag="ACFB2Q_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC0917942.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYHSSMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(14139..15002)
                     /locus_tag="ACFB2Q_000892"
     CDS             complement(14139..15002)
                     /locus_tag="ACFB2Q_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC0917943.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(15010..15627)
                     /locus_tag="ACFB2Q_000893"
     CDS             complement(15010..15627)
                     /locus_tag="ACFB2Q_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC0917944.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(15790..16404)
                     /locus_tag="ACFB2Q_000894"
     CDS             complement(15790..16404)
                     /locus_tag="ACFB2Q_000894"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC0917945.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRPQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            16611..18380
                     /locus_tag="ACFB2Q_000895"
     CDS             16611..18380
                     /locus_tag="ACFB2Q_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFC0917946.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            18377..19033
                     /locus_tag="ACFB2Q_000896"
     CDS             18377..19033
                     /locus_tag="ACFB2Q_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC0917947.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(19234..20237)
                     /locus_tag="ACFB2Q_000897"
                     /pseudo
     CDS             complement(19234..20237)
                     /locus_tag="ACFB2Q_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_304669545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         4670 a         5847 c         5065 g         4655 t
ORIGIN      
        1 gtgttacgca atatataagg ggttgccgtt ccgcagttgg gcggcagcat acacgttcct
       61 ttcggcgtat tctcgccgtt gcgcggcgaa tatctcgacc tgcttgccca tgcgccgtca
      121 acgggttttc agacggcatt cgatatcggg acgggctcgg gcgtacttgc cgccattttg
      181 gcgaaacagg gcattccttc cgtcatcggc acggatacca atccgagggc ggttgcctgc
      241 gcccgtgcca atattgcccg tttgggtttt gaaaaacagg ttgaaatccg cgaaaccgat
      301 ttatttcccg aagggtttgc cgacctgatt gtctgcaatc cgccctggct tcccgccaag
      361 ccgacttccg ccgtcgaatc cgcgttatac gaccccgaat ctgcgatgct ggctgcgttt
      421 ttgcgtgatg cgccaaacat ctgaatcccg acggagaaat ccgtcttatc atttccgacc
      481 tcgccgtgca tttggggctg cggtctgccg actttttgga aaaggcattt atccgggcgg
      541 gtttgcgtgt tgaggatgtt ttgaaaacca aaccggttca caaaaaagct gccgattcga
      601 acgatccgct ggcttttgcc cgaaacaggg aaactacttt cctgtgccgt ttgaaaaagg
      661 cataaggggc ggtggcgtgc ttccgacggt aggcatcgga acgcccgcgc cgccggcacg
      721 gcatcattcg ggcggattat tcttgtgaaa atacccgctc aagcatactg cccaacgccg
      781 tctgacggac tttgacggtg tttaccgcct gacaaaatgt ctttttgccg ccgaaaaata
      841 cgaatttttc cctggcgcgg gtaatggcgg tatataacag ctccttactc aatccggaca
      901 atgcatcgtc cccttcgtcc gaaggtgcgt cggaaggcgg cagcagccag acttcccgat
      961 attccgaacc ttggcttttg tggacggtca tggcgaatgc gggttcaaat tcgggcaggc
     1021 agcttaccgc taccttttta aatccgtccg catcggcaaa ataggcggca aggctgccct
     1081 gccgtccgac atcttccata atcagtccga tgtcgccgtt gaacagctca agcgcgtagt
     1141 cgttctgtct gatcataatc ggctctccgg cgaaataggc caaatgttcc ggtatgttca
     1201 ttttgcggcg tacatagctg caataggctt cgttgaagtc ttccgcatcc tgccgccaag
     1261 ctgccagaac cacgatatcc gagatgcctg cgtatgcggc ttcgatattg ccgtctttta
     1321 ccgcctgcca ataggctttg tgcgcccggt acaacctttc gacccgagca ttcggactgc
     1381 attccgaatg ttccagttcg tccggaaacc ggtcaaacaa tgcccacgcc ccttcatcgc
     1441 ccgatacggc ggcacgggca aggcagccga tgccgctgtt gtcgccgaag cggtggctga
     1501 acgacagatg ggcggtgttt tgcgccaaca cgggcggatt tgcgctgacg ctgaaaccgt
     1561 gttccggaag gaaaccggcc agcctttggt gcgtttctcc gtccaaaacg gttttttgcg
     1621 acaaaaccga cagcaccgcc ccgattccga cggacgggag ctggttttca tcgcccagca
     1681 gaatcacgcg cgcgccggtt ttgaccgctt tcaaaagttg cagcatcaat gcggtatcca
     1741 acatggaggc ttcatcgaca atcagcacgt cgaacggcag cggacggata tggtcgaacg
     1801 ccgcctgcat tttgggcggg ctcagcttaa gcagtcggtg gacggtttgc ccttccagtt
     1861 tgagcaaatg gcggcggacg gcctccggcg cgtcaaaacc gttgattgca cggtgcagcg
     1921 cgcgcgccat atgtgccgcc gctttgcccg tcggtgcggc aagcgcgata tggggaagat
     1981 tttcgttttc accgcaaatc agtgccagca gtttggcaac cgttgtcgtt ttgcccgttc
     2041 ccggccctcc ggtaatcacc ataaaaaact gcaacagtgc caaggcggcc gcatcgcgct
     2101 gtccttcgct tcccgcgcct tgaaaccatt ttgcgaggtt ttgccgcgcg cctgccgcgt
     2161 cgggggcgga cgtgccggct gccgccaagc gttttatttc ggcagccaaa tcgtattcca
     2221 actgccacat cctgcccaaa aacagccttc tgccttccaa aatcaaaggc gcggcggatg
     2281 ttccgaccac gggtgcgagt gccgacagcg cgtcagcctc gtccccgctc aaacggataa
     2341 acgaatgacc gttttgcaat gcctgaaaca ggcgttcggt acagtttgca agcacttcgt
     2401 tgcccgaacc cgcatagcgt tccaaaaaac ggattgccgc ccttgccgcc gcttgggcaa
     2461 attcatctgt ctgcagttcc atcgtattcc ttgtcgaaat gccgtctgaa ggcacggggc
     2521 ttcagacggt gcgtgttgtt tcggctgttt aggcgtttgc gccctgtctg gggtgcaggc
     2581 tgccgtcttt catgaccatc acgcgctcga agcggccggc gagttcgtcg tcgtgcgtta
     2641 cgaccaccag ccccgttccc aattccgttt tcagttccag catcatatcc aaaacattcc
     2701 ttgcgtttgc ccggtcgagg ttaccggtcg gttcgtcggc aagcaggcat ttgggttgcg
     2761 taaccaaggc gcgcgcaatg gcggcacgct ggcgttcgcc gccggacaat tcgcccgcac
     2821 ggtgcgtcga acggtgtttc agtccgacct tgtcgagcat cgccattgcc gtttctaccg
     2881 cctctttacg gctttttttg ccgatcagaa gcggcatcat cacgttttcc agtgccgaaa
     2941 attcaggcag aagatgatgg aactggtaca cgaaaccgag atggcggttg cgcaaatcgc
     3001 ccaaacgccg ctggtttaag gcacgcaaat cctcgcccat cagcagcacc ctgccttcag
     3061 acggcatatc cagcccgccc aaaatatgca gcagcgtcga tttgccgctg cccgaagagc
     3121 cgatgatgcc ggtgctttcc cccgtgcgga tttccaaatc caagccgtac agcaccggaa
     3181 cgtccaaacc gccgtcacgg tagcgtttgc ccacgccttc gcatttcaaa atcaaatcac
     3241 tcataacgca aagcctccgc cggttgggtt tttgacgcgc gccagctcgg gtacagtgtc
     3301 gccacgaaag acagccccaa ggaaatgcag gcaatcaggg caacgtcgcc catatcgaca
     3361 tcactgggca ggtagtcgat aaaataaacc tgcgaattga tgaggtggac accgagcagg
     3421 ttttcaaaaa acgccacgac cctgccgacg ttccaacccc aaagcacgcc gcagaccaca
     3481 cccgccagcg tgccgaaaaa gcctgaaaac gcgccctgca ccataaaaat cttcatcacg
     3541 ccggcagggg aaagacccaa agtccgcaaa atcgcaatgt ccgcctgctt ttccgtaacc
     3601 gccatcacca aggaagagac aaggttgaac gccgccacgg cgataatcag cgtcaggata
     3661 atgaacatca tccgtttttc cagttcgacc gcttcaaaat agctgcggtt gctgtacgtc
     3721 caatcgcgca cccaaaccgt gtccctttgc gcctccggaa tcagcgttgc cgtcaaggcg
     3781 ggggcgtttt gcggatcggc aagcttcagc cgcagccccg caacttcctt atccaagcgg
     3841 tacagtacgc gcgcgtcttg gatatgcgtc atcgccaatg agttgtccac ttcgtaaaca
     3901 cccgtcttga ccaggccgac cacggtaaac tgcttcaggc gcggcacgac tccggcaggg
     3961 gtaacgttgc cctccggcgt aattaccgta accttgccgc cgacttccgc ccccaaagcc
     4021 tccgccaaac cgacaccgag gataatgtca aactcgcccg gaatcaggtc ttcaaacttg
     4081 cctgccggca ttttgtcgcc gtattccacc actttgcgct cttcagacgg caaaatgccg
     4141 cgtatctgaa cgcccctgat ttcgcccgcg tttgccagca atgcctgatt ggaaacatag
     4201 ggtgcggcag ccaaaatgcc tttgcggttt tcggcaaacc gaagcaggtt gcgccaatcc
     4261 gtatccgtat tgtcgatata gccgatttcg gcgtgcggcg cgacattcag gagctgcccg
     4321 cgtatttctt tctgaaagcc gttcataacc gacaagacga caatcagcgc ggttacgccc
     4381 aaggcgattc cggcaatcga aaccatcgtg ataaacgaca taaagccgtt gcgctttttc
     4441 gccctgagat acctcaagcc tatccaagcc tctagagaaa acataacgct accttaaaaa
     4501 tgtctgcaaa cgtgccgccc cggacggcgg tttgggggag gcggcaaaag tttattgtac
     4561 cgtaaaaccc cggcagcgtc cgaacggcag gctgtgtaaa taaagacgcg gtatgtggca
     4621 aattcggaaa aacaggacgg aaaacttgca caacgcgccg aactacccta tccttctctt
     4681 gaaacaaaac cttttcttta aggaaaacaa tgaatatcag gaaaatctcc gctttgtgtg
     4741 ctgttgccgt ttttactgtt tcgacagcct atgccaaaga aatcaaaatc gatgccaaca
     4801 acacgcctta ttccgaagcc gacgcgcaaa agctggcggc aacggcagtc ggtatgggcg
     4861 ttaaggaacc tgtcagcctg aacggcggca gcggcagcat taccgtttcc ggcagcagcg
     4921 cggcgcagtg cgtctttacg gtcggcagcg ggggcacttt gcagattcaa gggctcaact
     4981 gcaagtaaac cgcccgaaaa aatgccgtct gaaggcttca gacggcattt tgcattggcg
     5041 gcgttacgcc ccgccttctt taatcagacg ccgttcgtac accgcctgcg ccagcgttcc
     5101 cgcatcgaca tattccaatt cgccgcccaa tgggatgccc tgcgacagcc tgctgacctt
     5161 gtaaggcagg tttttgaaaa actcggacag gacatacgct gtggcattgc cttctgcggt
     5221 aaaggcggtc gcgataatga tttcttcgat ttccccgccg tccagccgtt gcgccagcct
     5281 gtccaatgcg atggcggata cgtccattcc caatgccgta ttgatttgcc ccatcaggac
     5341 gaaatacagc ccgtcgtggc agtttgccgc ttctatattc gacacgtcgg caggcatatg
     5401 caccaccatc agccgccgcc cgtcgcgtgt ttcatcggca caaatatcgc acaatccgcc
     5461 ttcgcaaaat gtattgcacc ttgcgcaatg gcgaacctgc ctcaatgccg tctgtaaggc
     5521 atccaccagc tcttcagcct ctttgcgctt ttgctgcaac agatggtacg ccatccgctg
     5581 tgccgatttc ggcccgacgt tgggcaatac tttcagcgca ccgatcaggc gttggaatgc
     5641 atcctgtttt ttggaattca tcatactccg ccatatggga aaacggtcgg aatattccga
     5701 ccgttatgtt gtcaacaaaa gtgtcaatta ctgaccatcg ccgttgtcaa ccgattgtgc
     5761 tcctttagtc tgtttgattt ttccgttgaa atagcggatc agcaggtcga aagtattggc
     5821 agactgctgt tgcgccaaag cctgtttcgc aggaggaagc tgtgcggcaa tatcctccgg
     5881 aggcgtgact gcctgcacct cgacaatcac gggtgccggc agaccggtca gtctgacata
     5941 ggcgggtttg ccgtttgccg gttttgcttt cagcagttcc gcataagcct cgggcggcat
     6001 ggactgcctt gcctgctgcg cgcccaaaac ggacacttcc gaccatttca cgtcaactgc
     6061 cttgccgccg ttcagttggg taagcacttc ttttgccttg ttttcggcaa gtttggcggc
     6121 ttcggtacgg atataggcct gacgcaccgc atctttggct tcttcaaaca gtaggttttt
     6181 ttcttcgcgg acttctttgg cgcggacgac ccacgcggtt tcgctgttga tggtcagcac
     6241 ttcggaattg tgttttttct tcaatacgtc gtcgctgaat acggcattga ttaggttttc
     6301 gggcatgccg gacatttgtg cgtcctgcct gctcagccaa gtttcttggg tttccacttt
     6361 caaaccgctg tttttggcgg cttcggcaag cgaggaggga tgattgaacg catcgtcgcc
     6421 cagcttttct tttgccttgt tgaagtcggc aaccgccttt ttcattttca attcgttttc
     6481 gacggcggct ttttcctgct cgaaagaagg tttggcttca tgtgccggca aacgcgccac
     6541 gcgctcttca aacgcatttt tcacttccgt ttcactgacg gtctgcttgt ctgcaaaatc
     6601 cttcagattc aaggcgacat attccaattt gaccgcctgc ggcagcagat agtctttttt
     6661 gttcgcatta taaaatttct gcaaatcggc ttcagacgct ttgacttggg cgatgaactc
     6721 gtcggggttg aaagtgtgcg aacggatggt gcggttgacc tgcgtcagcc tgatcagctg
     6781 ttccgcctgc gcgtcgccga ccaatacgcc gttttggacg aggcttacca aattctgcaa
     6841 ggcaaactga tcgcggattt cttcgacaaa ctggtcttca gacatatggc gttgcgacag
     6901 gtattgactc aaaagcgcgt gactgaattt gccgtttgcg tcgtggaaat tgggatcgtc
     6961 cacaatcatc tgcttgattt gttcggaaga aaccgaaatg cccatcagct tcgcaccctg
     7021 tttcaggtag gcgcgttgca gcagggattg gaacaccgcg tcgcgccaag ggctgccgcc
     7081 gtccgcctgc tcgttctgca tggcgttgtt gattgagtgc tcgctgattt tttcgtcgcc
     7141 cacttggacg atgtagtcgg cgcccggatg ggaaaccgtg ctgacgccga agccgacaaa
     7201 agttaatgca atcaggccta ataagacttg ggcgggtgtt ctgtattttt cgatggaatg
     7261 gaacatattt tcagattggg aatgggaaat tcaagtcggg tattgtaacg gtttttatcc
     7321 ctgtctgcac ggggcttgcc ggttgaagat gccgtcgtag gtttcttcgc tgaagccgac
     7381 gtaaaccctg ctgccgcact ccaatacggg acgcttgatc aggctcggca tttcggacat
     7441 cagtttgacg gcctccgccg tcgaggacag ggctttttgc tgtgtttcgg catcgagttt
     7501 gcgccagctt gtcccgcgtt tgttgaacag ggttgccaaa ggcacttgtt ccagccacga
     7561 gcagatttcc gcttcagacg gcatctgttt tttgaaatcc cgaaacccaa actccaagcc
     7621 gtatccggca agcctgtttt tggctttttt gactgtgtcg caatttggaa tgccgtgaag
     7681 gattatcatt tggaaacctt ttgcctgaaa taataaaacc gatattttac tataagtgtc
     7741 tgaaaatttg cccgtctgtt tcagacggcg gggcggttat gttacaatcc gaaaattcga
     7801 aaaatttaat ctcttgttca ataaaggttt tacccataat gatttctacc aacggcatta
     7861 ccatgcagtt cggcgcgaag ccgctgtttg aaaacgtatc cgtaaaattc ggcgaaggca
     7921 accgctacgg cttgatcggc gcgaacggtt cgggcaaatc caccttcatg aaaatcctcg
     7981 gcggcgattt ggaacagacc gccggcgaag tggcgattga aaacggcgtg cgtttgggta
     8041 aattgcgcca agaccagttt gcctacgaag acatgcgcgt gctggacgtg gtgatgatgg
     8101 ggcataccga aatgtgggcg gcgatgaccg agcgcgatgc gatttacgcc aatcccgaag
     8161 ccaccgaaga cgactacatg aaagccgccg aactggaagc caagttcgcc gaatacgacg
     8221 gctacaccgc cgaagcgcgc gccgccgaat tattaagcgg cgtgggcatt tccgaagatt
     8281 tgcacaatgc gaaaatggcg gaagtcgccc cgggcttcaa actgcgcgta ttgctggcgc
     8341 aggcgctgtt ctccaagccg gatgtattgc tcttggacga accgaccaac aacttggaca
     8401 tcaataccat ccgctggctg gaaggcgtgt tgaaccaata cgactccacc atgatcatca
     8461 tctcgcacga ccgccacttc ttgaacgaag tttgcaccca catggcggat ttggactaca
     8521 acaccatcac tatttatccg ggcaactacg acgactatat gctcgcttcc gcccaatcgc
     8581 gtgagcgcgc cctgaaagac aatgccaaag ccaaagagaa actgcaagag ctgcaagaat
     8641 tcgttgcccg tttttccgcc aataaatcca aagcccgtca ggcaaccagc cgtctgaaac
     8701 aggccgacaa aatcaaatcg gaaatggtcg aagtcaaacc ttctacccgt caaaacccgt
     8761 atatccgttt tgaagccgat gaaaaagcca agctgcaccg tcaggctgtg gaagttgaaa
     8821 aactggccaa acgctttgaa acccagttgt ttagaaacct caacttcatc cttgaagcag
     8881 gccaacgcct cgccatcatc ggcccgaacg gcgcaggtaa atctaccttg ctgaaactct
     8941 tggccggcgc gtacaacccc gaatattcag acggcctgtt gccggacgaa ggcagcatca
     9001 aatgggcgga aaaagccagt gtcggctact atccgcaaga ccatgaaaac gactttgacg
     9061 tcgatatgga cttgagcgaa tggatgcgcc aatggggtcg ggaaggcgac gacgaacaag
     9121 tcatccgagg cactttgggg cgtttgcttt tcggcagcaa cgacgtggta aaaaaagtga
     9181 aagttctctc cggcggcgaa aaaggccgta tgctttacgg caaactgttg ctgttgaaac
     9241 ccaatgtctt ggtcatggac gaaccgacca accatatgga catggaaagc atcgaatcgc
     9301 tgaacatggc actggacaaa tacaacggca cgctgatttt cgtctcccac gaccgtcagt
     9361 tcgtttcctc cctggcaacc caaatcatcg aactggacgg caaaggcgga tatgaacact
     9421 acttgggcga ttacgaaagc tatctcgaga aaaaaggcgt agcataagcg cacccctccg
     9481 gttgaaacaa tgccgtctga agccgcttca gacggcattg ttgataactt taaaatagga
     9541 agcatatgca gacttacctc gtcggcggcg ccgtccgcga ttatcttttg ggcctgcccg
     9601 tcaaagaccg cgattgggtg gtcgtcggcg cagacgcgca aaccatgttg gcgcaaggtt
     9661 tccagccggt cggcaaagat tttcctgtat tcctccatcc caaaacgcac gaagaatacg
     9721 ccctagcccg caccgaacgc aaaaccgcca agggttacgc cggtttcagt ttccacgccg
     9781 acaaagacgt tacgctggaa caggacttga tgcgccgcga cctgaccatc aacgcgatgg
     9841 cgcaagatgc ggacggcaag attatcgacc ctttcggcgg gcaacgggat ttggcggcag
     9901 gcattttgcg ccacgtctca cccgccttcg ccgaagatcc cgtccgcatc cttcgtgccg
     9961 cccgctttgc cgcgcgttac ggctttgaaa tcgccgaaga aaccataaag ctgatgcggc
    10021 agatggtgga aaacggcgaa gcggacgcat tggttgccga acgcgtctgg caggagttgg
    10081 cgaaaggttt gatggaaaaa aatccgcgca aaatgattga aatgttgcgc gaatgtggcg
    10141 cactccaagt cttgctgccc gaagtcgacg ccctcttcgg cgtgccgcaa cgcgccgact
    10201 atcatcccga aatcgacagc ggcatccata ccctgatgac gctgcaacgc gccgccgata
    10261 tgggtttgag cctgcccgaa cgctatgccg ccctgctgca cgacttgggc aaagccaaaa
    10321 caccgcccga catcctgccg cgccaccacg gacacgacat caacggcgta gaacccgtgc
    10381 gcgaagtcaa tcaacggctg cgcgcgccga ggcagtgtgc cgagcttgcc gaattggttt
    10441 gccgttggca cattattttc caccaagtcg gacagcttaa aagccaaacc attctgaacg
    10501 tattgaaaaa aaccgatgcc ttccgccgtc ccgagcgttt tcagacggca ttgaacgtct
    10561 gcattgccga cacgcaaggc cgtctgaacc gagaacacac gccctacccc cagcgcgcgc
    10621 actggctcgc cctgctcgaa gccgccaatc aggcggattc gggcaaaatc gccgccgaat
    10681 gccgcgcgca gggaaaagcg cactttatcg ccgaacaaat cgaccgggcg cggctggcgc
    10741 aaatcgcccc gctgcaaaaa gcgtttcggg gcgcgtagga aaaagaaaac gccaaacgcg
    10801 gcatcttttg aggttcgccc gcattgccga aacagcgtcg gagaaaggga tggatgacat
    10861 cggtttattc ggctgccttt taatattccc ttgatgcagg cctgattagt cggacaaaaa
    10921 taccttttgt tcaacggaac cgatttctat ccggacggaa acagccggct ttccccttgc
    10981 aaacggatgg aatcaagcgg gtattcaaac acagccgttt atttttattc cgccgccaag
    11041 ttatgggata gggtttgcgt gtttcgggca ggcaaaccgc gcaaccttag acgcagtccg
    11101 aagttttgaa ggcggcatcc gggccgggtt ttaaaacagc cgccgccata tccgctataa
    11161 tccatccttc agccattccg cccccgacat aaaatcatga ccctgaaaac cgatttattg
    11221 cctaaaatca acaacgaaga ttatcaacgc ctcatcctca aacatagcgc ggaattcagc
    11281 ggcggagaaa cccgcctgtt gaacgaaatc ctcgaaaaat tcaatttcga cgttgttcag
    11341 gcgcaggcat tggcgcaggc ggtaatgcag caagttcgct tcgatcccaa cgcctaccac
    11401 atcgacagcg acgacgaaga caccaccggc atctgccccc actgcatcaa cccgcctatg
    11461 ccgcccctgc gcgactatct cgtttggcgc gaaacacgcg gataaaacgc ttttgaccgt
    11521 tatcttttca atgccgtctg aaacgccgcc gaccgttcgg acggcatacc cgacaaaggg
    11581 aacactatgc tgcaaaccga caacctgacc gccgcgcaac cgcaacgcat cgttgccgcc
    11641 caaaccgcct ccgcacagga agaactgctc gaacgcgccc tccgccccaa aacgctggac
    11701 gactacatcg ggcagcataa agccaaagag cagctcgcca tcttcatcca agccgccaaa
    11761 aaacgcggcg aagcactcga ccacgtcctg cttttcggcc cgcccggact gggcaaaacc
    11821 acccttgccc acatcatcgc caaagagttg ggcgtaaatt tgcgccaaac cagcggcccc
    11881 gtcctcgaac gcgccggcga cctcgccgcc cttttgacca accttgatcc gcacgacgta
    11941 ttgtttatcg acgaaatcca ccggctaagc cccgtcgtcg aggaaatcct ctatcccgcg
    12001 ctcgaagact accggctcga cattatgata ggcgaaggac ccgccgcacg ttccgtcaaa
    12061 atcgacctgc cgcccttcac cctcgtcggc gcgaccaccc gcgccggtat gctgaccaac
    12121 ccgttgcgcg accgcttcgg catcgtctcc cgcctcgagt tttacgaaaa ccgcgacctt
    12181 accaccatcg tcagccgttc ggcacaactg ttgcagctcg atatgggcga agaaggcgcg
    12241 atggaagtcg ccaaacgcag ccgcggcacg ccccgcatcg ccaaccgcct gttgcgccgc
    12301 gtgcgcgatt tcgccgacgt gaaaaacaac ggcgtaatcg atgccgccgt cgccgatgcc
    12361 gctttaagta tgctggacgt ggacgcgcag gggctggacg tgatggacag gaaattcctc
    12421 gaagccgttt tgcacaaatt cggcggcggc cccgtcggtt tggacaacgt tgccgccgcc
    12481 atcggcgaat ctacggacac catcgaagac gttatcgaac cctacctcat ccaacaaggc
    12541 tttttgcaac gcaccccgcg cggcaggatg gcgaccgaac gcgcctacct gcatttcggg
    12601 ctgcccgtcg aaaaataaac catgccgtct gaaaccgaac cgggtttcat acggcattgt
    12661 tatcagacgt ttgcaccggc accggtttca tcacttaaca gaaacctttt gtccggcagg
    12721 acagattctc cccgatgaaa ataactgttt ccctggcaaa cggttgaaac ccgtctggct
    12781 gccggttgaa aaataacgca atgctgtctg aaacagagct aattttcaga cggcatttct
    12841 atttcaatca ttggctcaag gttcagcctg ccgctttttc cagttccgcc ctcatcgcag
    12901 cgattaccgc cttgtaatcc ggtttgccga aaatcgccga accggcaaca aaggtatccg
    12961 caccggctcg ggcaacggcg gcaatattgt cagttttgat gccgccgtcc acttcaatgg
    13021 caatacgtct gccgcttttc ccttcatagc ggtcaagcat ggcgcgaacc tgtctgattt
    13081 tttccaaagt gtgcggaatg aagctctgac cgccgaaccc ggggttgacc gacatcagca
    13141 aaaccatatc cagcctgtcc aacacgtttt ccaacacata tacgggcgtt gccggattca
    13201 acaccagccc cgcctgacag cccatgtccc gaatcaggct caagctgcgg tcgatatggc
    13261 ggctcgcttc gggatggaaa gtgataatcg atgctcctgc tttggcaaac gattgaatta
    13321 aatcgtcaac gggttcgacc atcagatgca catcgatcgg cacgcttgca taaggcttca
    13381 acgccgcgca gaccataggt ccgaaggtca ggttcggcac ataatggttg tccatcacgt
    13441 caaaatggat cagatccgca cccgccgcaa tgacatttgc cacctcttct ccgaggcggg
    13501 caaagtctgc cgacaaaata ctgggtgcga tacggtaggc ggtcatattt tttccttcaa
    13561 tgcctttatg ggcggactca acgcgggaaa gcctcgaaac agcattatta acaaaagtta
    13621 actgccataa taccatcttt cacacggcaa tcaagtatat tgtccgaaat atccggatta
    13681 aaggaaaagc cgtgccgctg cctgctccct gccgttttgc caaacctgcc gcctcttttt
    13741 taagtatggc tttgctttcc tgccagcttt cccacgccgc cacggcttat atccccccga
    13801 acgattttca accgaactgc gacatacgcc ggctcgggct gacacagggt cagcacaatg
    13861 agctgcgtaa aatccgcgcc gccttcaaaa tggcgggcga cagggcgcgt ttgaaggtta
    13921 tgcattccga acacagccgc cgccgctctg tcgtcgaaat catttcttcg gatgttttta
    13981 atcggaacga ggcgcgcgat tatgtcgaaa gccgctacca ctccagcatg gattttgcgg
    14041 tggacgaatt ggaaatccaa caccgcttct tccatattct cacaccgcaa cagcagcaaa
    14101 tgtggctttc ttcctgcctc aaataatccc cgaaacactc acggcgcacg ttgctccggc
    14161 agcctgcccg cccagtcgca ggcaaactgc caagcggaac gtcccgagcg gttgccccgc
    14221 atctgcgccc actgcaacgc cgccgtccgg gcggcttcgt catatggcac atcaaaatct
    14281 tccaaccagc ttcgcactgc cgccagataa tcgttttgat cgaacggata aaaactgagc
    14341 cacaatccga atcggtcgga caaagatact ttttcttcca cggcctcttt ttgatggatt
    14401 tctccgcgca cgcctgtcgt acctgcattt tcatccaaat attcgggcat cagatgccgt
    14461 ctgttggaag tcgcgtacac catcacgttg gcgcaacgtt gggacagacc gccatccaat
    14521 gccgttttca atgccttata ggtttcgtcg ccgctttcaa atgacaaatc gtcgcaaaac
    14581 acaatgaatt tttccgggcg ttccttcaaa agtgtcaaca gatacggcag gccgatcaaa
    14641 tcgcttttat cgacttcgat caggcgcaat cccttatccg catattcgtg cagcagggct
    14701 ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca tcaatacgtt attcgcaggt
    14761 ctgccggcaa taaattgttc ggtattacgc accagcaatt cggtctgcct gccgactccg
    14821 gcaagccgcg acaaagggaa aacgtgcgga tcgggcaagt gttccaaaaa accttttttg
    14881 cccgcactct gccagcggaa ggcaagcgcg ttccaatccg tatgcccggg ttcgggcgga
    14941 agcacggcat ccaaacgccg caaaacggca taggctttat cgagaaattc gttcaattcc
    15001 atctctacct cactttgcat atctttgcgc catcagccgt tcgacggtgt cgacgattgc
    15061 ctgcgtattc ggatcgattt cgatgttgat cctgtctccg gcctttctgc tgccgaacag
    15121 cgtccgttcc aaagtttcgg gaatcagatg gacattgaaa cggctgtctt cgactttgcc
    15181 tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg gtcaggatat agggtttgag
    15241 ttcgtgcggg agcgcaaacc aaaccgtgcg gttgaacccg tcccgttcga tttcgacaat
    15301 gggcacggtt gccataatgt gtccgctcat gacgtgtccg ccgatttcgt cgccgaaacg
    15361 tgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc agccccaaat tggtttttgc
    15421 caaagtttct gccattaaat cgaagctgac gcggtttcct tcgatttcag taatcgtcag
    15481 gcagcagccg ttattggcga ccgatgcgcc gtgttgcagg ttttctgccg cctcttgcgg
    15541 aagctcgacg acataagtct gaaacgcctc cgacgggcgg tggattgccg tcagttttcc
    15601 caatccttga acaatgcctg taaacataat cctgtttccc tgtgtcggta aaaatggtgc
    15661 aaattgtagc atttccccgc gaaaaatgcc gtctgaaact ccttcagacg gcattgtgcc
    15721 tccggttcgg gcaaaaaccg cccggtacgc cttgaccttt cctttccgcg ccggtcggcg
    15781 gtcttgcact tatccctcct gcaaatcggt ttgcgtgttc aagtcggcaa aatgcccgtc
    15841 aaactcgaat ctgaccggcc gcgccctttg ctgctgcaac cagcttctta atgttttcat
    15901 acccgaaaac agataaggaa tcgcgctttg cagaatttgc ggacggatat acataatgtt
    15961 gtagtgcatc gttaccggcg tttccacata aaacgcattg cacaacggtg tgcgtttgga
    16021 gacgctttca aagcgcgcga ccaaatcgtc cggcagatac ggcatatcgc aaggtacaat
    16081 cagcagccaa tcggccgccg ccaactgcaa atcgttggct gcggtacaca atgccgaaag
    16141 cgggccgaaa tgctgccact gccgggcatc cggaaaaaca tgcgggctgc ggcgggcgta
    16201 ttcttccaaa ttccggttgg cactgatgac gatatggctg acctgcggcc tgattttacc
    16261 gataacgcgg tcaatcagcg gtctgccttc caacagtgcc agccctttgt cttccccgcc
    16321 catacggtcg gccaggcctc ctgccaaaat caatgcgaac gtttttaatg cgggcgggct
    16381 tttgggaaag tttgaggttt tcatagtttc agtctgccgt ttccgatatt ttagaagcgc
    16441 gtttgaaaac ggcgtcgtat aaattcatgt tatttgaatt ttatcataac gtgatttaac
    16501 actgaaagca ttgaggctta agttttcctt aacctgtcct ttctgagcag ttgtttctaa
    16561 ttccaaagaa tgatattgtt tgcaccgttt aaaacgactt tcctccgaat atgatactgc
    16621 caacccgatt ttcagacggc atcccccttt ccctgcgcct gaaactcctg accggcttgt
    16681 gggtcggttt ggcggcactg tctgtcgttt taacgctgct gctttctttc cgcctggaaa
    16741 atgccgcctc ggtcatcgaa gaggcgggca atctgaagat gcaggcatac cggcttgcct
    16801 atatggcggg cgaaggctcg ccccgcgcac aaatcgacaa tcagattgcc gaatttgaaa
    16861 aaagtttaaa acgcatttcc caaagcgatg ccatccatcc gctgattccc tccgacaatc
    16921 cccttgccta cgatttgata cagtccatgc tgattatcga ctggcaggcc aacatcctcc
    16981 ccccgttgca ggcataccgc cgccccacac aaatcgagct ttaccgcttt gccggcaata
    17041 tcgaactgtt tttgcaggcg ttggaaaatg ccggcgaaaa aaacacttgg tggctcaggc
    17101 ggtttcaatg ggtcattatg ctgatgacgc tggtgtcgtc cgtgctgatg cttttttggc
    17161 accagatttg ggttatccgt ccgctgcagg cgttaaggga aggtgcggaa cgcatcggtc
    17221 agcggcattt cgacatccct gttcccgaag acggtacgcc cgaattcaaa caggtcgggc
    17281 ggtgtttcaa ccaaatggcg ctcaggctga agactttata cgatgatttg gaaggacagg
    17341 ttgccgaaca aacccacaac cttgaaaagc aaaaccgcaa cctgaccctg ctgtaccgga
    17401 ccacgcgcga cctgcatcaa tcctatacgc cgcgacaggc ggcggaagaa tttttgaacc
    17461 acatcctgcc cgccgtcggc gcgcaatccg gcaacatctg cctggaaaac ggttccgaca
    17521 cagacatttc cgtccatacg gccgaacacg gcaaaaagcc ccctttggaa aaataccatg
    17581 acgaaacctt ccccatcgaa tatcaaaacg agaaactggg catgctttcg ctcggctttt
    17641 cagacggcac ttccctcacc ggagacgacc gcacgctgct tcaaaccctg attcggcaat
    17701 tgggcgtatc gctcgccggg gcgaaacagg aggaagaaaa acgtctgctt gccgtattgc
    17761 aggaacgcag cctgattgca caggggctgc acgacagcat cgcgcaggca ttaaccttcc
    17821 tgaacctgca ggtacagatg ctggaaaccg cctttgccga aaacaaacgg gaggaagccg
    17881 cagaaaacat cggctttatc aaaacaggcg tgcaggaatg ttatgaagat gtccgcgaac
    17941 tgctgctcaa tttccgtacc aaaatcagca ataaagaatt tcccgaagct gttgccgacc
    18001 tgttcgcgcg cttcacgcaa caaaccggga taacggtcga aaccgtttgg gaaaacggtt
    18061 cgttcctgcc tacacaggac gagcagctcc aaatgatttt catcctgcaa gaaagcctgt
    18121 ccaacatccg caaacacgcc cgcgccaccc atgtaaaatt caccctttcc gaatacggcg
    18181 gacgctttac catgaccatc caagacaacg gacaaggttt cgacacggag aaaataggag
    18241 aacctacggg cagccatgtc ggactgcaca tcatgcagga gcgtgccaaa cgcatccgtg
    18301 ccgttttaga aatccgttcc caagcacaac agggaaccac cgtctcattg acgggcgcac
    18361 ccaaagaaag cctaccatga ccatcaaaat catcctgata gacgaccaca ccctcttccg
    18421 cagcggcatc aaagcccttt tgtcgcgcca acacggtttt gaagtcatcg gcgaagccgc
    18481 agacggactc tcgggcatca aaatgatcag tcggctgcaa cccgatgtcg tcctgctcga
    18541 tttggatatg ccaggcatga acgggcgcga ggccttgtcc caaatcatca gcatcaatcc
    18601 gcagcaggca gtcattatgc tgaccgtttc cgaagacagc gacgacctta ccgaatgtat
    18661 gcgcatcggc gcgcgcggct acctgctgaa aaacatcaac gccgattttc ttctcgaaag
    18721 catacgcaaa gccgccgaag gcgataatgt attctcgccc gagatgaccg ccaaactcgt
    18781 caaaagcctg atttcccccc aacctgccca aaggacgcag gcactctcct cacttacccc
    18841 tcgtgaactg gaaatcttgg gctatctcgc cgcaggacac agcaacaaaa tcatcgcccg
    18901 ccacctcgat cttgccgaat ccaccgtcaa agtccacgtt caaaacctgc tccgcaaact
    18961 caacctcagc agccgggtgc aggccgccgt ttacgccatc cggcacaacg tcccccaacc
    19021 tgtgccggaa tagacgttca gacggcatcg ggtcaggaaa aaagcaatgc gcccctttga
    19081 cggggtgcaa tatataaggg acggcacacc ggatgccgtc cctttaagta cgccattacc
    19141 gccgtaaccc ggacggaatg gcttaggcag tttatttcca agaatatttc acacccacgg
    19201 tgccttggta gcctttatgt ctgttgcttc cgcatcctgc cgataattgc cttgcctgtc
    19261 ccgagaaagg gtttgatttt cgccacggcg ttggtggagc gtatccagca tcgcatagcc
    19321 tgattccaag ttttgacgtg gaacgagtgc gtaggccggt gcgcttggcg tgatgatggt
    19381 tttatcttga ttcggcgtgg ttaaagtcca gaagtattct gtcgcgcccg cctcatcttt
    19441 gtgtgaaacc aattgcaatt cccccgcccc tgtggttttg gcagttccgc taaaagtatc
    19501 gcgcattgtg taggtatgtt tagcgtcttc gatatttaat ttaccggttt ccgccccctt
    19561 gtcttgtccc aaaaccttaa caacggcggc actgcgtttg tagcggtcgg aaagttcgcc
    19621 gatcgagccg tcgataatat tttctttgcc atctttaccc actgaaatga ccgttgtttt
    19681 tcctgaagca tcgcctgtga tttccagtaa atcagattga gaatttcctc cttgatcatc
    19741 acccggcgag ttccattctg tattgacttt taacacaccg tctttaccga cataatttcc
    19801 ttcaatggtg agtttgtctg cataggattg attggcaagc gtaatgatgc cgccgttggt
    19861 gaatgttcct ttttcaaata cagaagttgt acccgataaa gaaatacctg tattagatag
    19921 ggttagcgtg ccgcctgata ataatgtagt cccttcgggt gttgttaatg atgtgtcttt
    19981 ttttaggtga aaagtagctt ttgttagttt tatggttcca atatcttctg atttgaattc
    20041 cattgcgcca tttgtaattt ttagcacagc attggtaata gggaggggga agcgcattgt
    20101 aaatgatttt tctgttccat ctggattgat taatgtacca gatgcgccaa agatctctcc
    20161 ttcagggata gaaacagttc catcagaaat tgatggttta tttgcgcttt tatcactgtg
    20221 tttatgaatt aatgtaa
//
LOCUS       DAXDSD010000032        20076 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXDSD010000032 DAXDSD010000000
VERSION     DAXDSD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20076
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.032"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            282..1754
                     /gene="pyk"
                     /locus_tag="ACFB2Q_000898"
     CDS             282..1754
                     /gene="pyk"
                     /locus_tag="ACFB2Q_000898"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC0917948.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            1988..3340
                     /locus_tag="ACFB2Q_000899"
                     /pseudo
     CDS             1988..3340
                     /locus_tag="ACFB2Q_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
     gene            complement(3423..3890)
                     /locus_tag="ACFB2Q_000900"
     CDS             complement(3423..3890)
                     /locus_tag="ACFB2Q_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004466844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917949.1"
                     /translation="MSIRQGKPEEEFSRKMAGCLTDLENCLEKYLEQFGPVSESIEAC
                     TAKLQEQPSFFNRLMKANDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSIHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLYIFSRIPR"
     gene            complement(4028..4177)
                     /locus_tag="ACFB2Q_000901"
     CDS             complement(4028..4177)
                     /locus_tag="ACFB2Q_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917950.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVSYADVSTHRTAHKAS
                     PYVPS"
     gene            complement(4195..4392)
                     /locus_tag="ACFB2Q_000902"
     CDS             complement(4195..4392)
                     /locus_tag="ACFB2Q_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917951.1"
                     /translation="MNTVPKSRIPVKPLPEKTTAEAKVEKWRQLGADHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(4402..5418)
                     /locus_tag="ACFB2Q_000903"
     CDS             complement(4402..5418)
                     /locus_tag="ACFB2Q_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC0917952.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQDIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSPLLYGETSLADIVQQKTGGNVEFKDGVLTAAVRFLPAKDARTAFIDNTV
                     GMATQTLSAALLPYGVKSIVMIDGKAVTKEDAVRVLSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDVERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(5517..6731)
                     /gene="gltS"
                     /locus_tag="ACFB2Q_000904"
     CDS             complement(5517..6731)
                     /gene="gltS"
                     /locus_tag="ACFB2Q_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC0917953.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(6984..7265)
                     /locus_tag="ACFB2Q_000905"
     CDS             complement(6984..7265)
                     /locus_tag="ACFB2Q_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917954.1"
                     /translation="MKQIKASDFTDEHFGLPTILDILSELEKPQPRTDSRPQTTTQRA
                     CPNQFGDGGCVCEVEAVSPTAGMYSDRCSGGFCPRFFCYFKYVYSHDAV"
     gene            complement(7284..8408)
                     /locus_tag="ACFB2Q_000906"
     CDS             complement(7284..8408)
                     /locus_tag="ACFB2Q_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC0917955.1"
                     /translation="MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQ
                     DQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTP
                     LSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNK
                     KEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLK
                     RVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLA
                     YPVLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSD
                     DVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
     gene            complement(8471..9478)
                     /locus_tag="ACFB2Q_000907"
     CDS             complement(8471..9478)
                     /locus_tag="ACFB2Q_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC0917956.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYRKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLVEN
                     T"
     gene            complement(9471..10472)
                     /locus_tag="ACFB2Q_000908"
     CDS             complement(9471..10472)
                     /locus_tag="ACFB2Q_000908"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC0917957.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(10503..12776)
                     /locus_tag="ACFB2Q_000909"
     CDS             complement(10503..12776)
                     /locus_tag="ACFB2Q_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC0917958.1"
                     /translation="MNITRILSQELSATTVQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENIPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHTQVVEGKETEGEKFSDYFDRREPVRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPESSLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDANTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRD
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            12891..13907
                     /gene="galE"
                     /locus_tag="ACFB2Q_000910"
     CDS             12891..13907
                     /gene="galE"
                     /locus_tag="ACFB2Q_000910"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC0917959.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            13908..14948
                     /gene="rfbB"
                     /locus_tag="ACFB2Q_000911"
     CDS             13908..14948
                     /gene="rfbB"
                     /locus_tag="ACFB2Q_000911"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC0917960.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            15053..15919
                     /gene="rfbA"
                     /locus_tag="ACFB2Q_000912"
     CDS             15053..15919
                     /gene="rfbA"
                     /locus_tag="ACFB2Q_000912"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC0917961.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYRVKDPERFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFHDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTQESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            16057..16614
                     /gene="rfbC"
                     /locus_tag="ACFB2Q_000913"
     CDS             16057..16614
                     /gene="rfbC"
                     /locus_tag="ACFB2Q_000913"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC0917962.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRIVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(16658..18676)
                     /locus_tag="ACFB2Q_000914"
     CDS             complement(16658..18676)
                     /locus_tag="ACFB2Q_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC0917963.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVAVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAALQATLMT
                     TIASGIFAHNLEWAYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(18871..19992)
                     /gene="dnaJ"
                     /locus_tag="ACFB2Q_000915"
     CDS             complement(18871..19992)
                     /gene="dnaJ"
                     /locus_tag="ACFB2Q_000915"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC0917964.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         4972 a         5521 c         4893 g         4690 t
ORIGIN      
        1 ggcttagacg actttttctc atagggataa ttctaactta atttgaattt ccctagttat
       61 ctaggacagc cccaaaataa atttagccta aatcataaaa ccgtgtaatt ttgcaccaaa
      121 aaaggcggtt tgcaggtaaa atatcaaacc attaatttaa atcatatttt tacagaatat
      181 tccgccgcgt tcaccaaatg gacatcaaac cggttccaaa tttctgtaat tttgcaacaa
      241 aataccgcaa aacacccgat tgagaccgaa aggactttca tatgaaccag acaagccgcg
      301 atctgacccg catcagccac aacaccaaaa tcgtcgccac ccttgggccg ggcagcaaca
      361 acgtcgaact gttggaagac atgatccgcg tcggcggcct gaacgtcgtc cgcttcaact
      421 tcagccacgg cacgcccgaa ttccatcagg aaaacgcccg catcgtgcgc gaggcggcaa
      481 aacgcgccgg acaggaaatc gccatcattg ccgatctgca gggcccgaaa atccgcgtgg
      541 gcaaaatcgc cggcggcggc atcgaattga acaaaggcga aacattggta ctcgatgccg
      601 cgctcgaagg cgaaggcacg cgcgaggcgg tcggtttgga ctaccgcgac ctgcccgacg
      661 acgttgccgc aggcgatgtc ttgtggctgg acgacggcct gctgaccctg accgtggaat
      721 ccgtcgaagg cagcaggatt atcacaaggg tggaaaacag ccacatcctg aaaagcaaca
      781 aaggcatcaa caaacgcggc ggcggtttgt ccgcaggcgc gttgaccgaa aaagacttcc
      841 gcgacctgaa aaccgcgatt gccatcggtt gcgactatct cgccatcagc tttgtgaaat
      901 ccgccgaaga cctgcacatc gcgcgcgcca aagtcgaaga ggaaatgaag ggcagcactg
      961 ccgtccgccc cggtttggtt tccaaaatcg aacgcgtaga agcgattgaa aaccttgatg
     1021 aaatcatcct tgccggcgac ggcattatgg ttgcgcgcgg cgacttggcg gtcgaagtcg
     1081 gacacgccgc cgtccccgcc ctgcaaaaac ggatgatccg ccgcgcccgc gagttgcgcc
     1141 gcttcagcat tacggcgacg caaatgatgg aatcgatgat taccaacccc gtgccgaccc
     1201 gcgcggaagt cagcgatgtg gcaaacgcgg tattggacgg caccgatgcg gtgatgtgtt
     1261 ccgccgaaac cgccgtcggc gcgtatccgt ttgaaaccgt cagccaaatg gcgattatct
     1321 gcgcggctgc ggaaaaagaa caggattcgc tcaacggcgt tgccgaacag acagagtatc
     1381 ccgaagccgt cagcaccaac ttagcgattg ccggcggcgc ggtcagcgtg gcgcgcgcgg
     1441 ttcacgccaa agccatcgtc gccctgaccg aaagcggttc gaccgccttt gaaatcagcc
     1501 gccacaatat taccctgccg attttcgcgc tgaccccgag cgtttccgcc caacgccgta
     1561 tggcgatgta ccggggcgtg cgcccgctga ttttggcaac cagcaccgac cacgacacgg
     1621 cgttgaacga agtcgaaacg atgctggtgg aacacaacat cctgcattcc ggcgaccagt
     1681 acatcatcac cagcggctcg caaatgcgcg aatccggttc gaccaatacg ctggaagtgc
     1741 tgcgcgtcaa ataatccgcc ctgagtggaa aatgccgtct aaagccgata ctgtcgggct
     1801 tcagacggca ttttatttgc attccgtcag gtaaaaccga aagaattgtg caaatccggc
     1861 aacatcggat aaaatcgagt gcctatacta aagcgaaaca aggcatttcc gactgccttt
     1921 tttatttgtc caccgtccgc ctttttacgg aaaccgaaat gacccctttc acactgaaaa
     1981 aaccgtcctg ctgctcggca ctgcctttgc cgccgcatct gtccacgcat ccggctacca
     2041 cttcggcaca cagtcggtca acgcgcaaag cacggcaaat gccgccgacg cgtcgaccat
     2101 cttctacaat cccgccggcc tgaccaaact cgacagcagc cagatttccg tcaacgccaa
     2161 catcgtgctg cccagcattc attatgaagc agattccgcc accgacttta ccgggcttcc
     2221 cgtccaaggt tctaaaaacg gcaaaatcac caaaaccacg gtcgcacccc acatttacgg
     2281 cgcatacaaa gtcaacgaca atctgaccgt gggcttgggc gtgtacgtcc ccttcggctc
     2341 tgccaccgaa tacgaaaaag attccgtgtt gcgccacaac atcaacaaac tcggtctgac
     2401 cagcatcgcc gtcgaacctg tcgccgcgtg gaaactcaac gaacgccatt ccttcggcgc
     2461 aggcatcatc gcccaacata attccgccga actgcgcaaa tatgccgact gaggaatccc
     2521 aaaaaaagcg caaatgctgc aagcaacacc ttctaatcct actgccgctg ctcaaatcaa
     2581 ggccgacgga cacgccgatg tcaaaggcag cgattggggc gtcggctacc aactggcgtg
     2641 gatgtgggac atcaacgacc gcgcgcgcgt gggcgtgaac taccgttcca aagtttcaca
     2701 cacgctcaaa ggcgatgccg aatgggcggc agacggcgcg gcggcgaaac aacagtggaa
     2761 tgacaatatg ctcacaccgc tcggttacac ggcgaatgaa aaagcccgcg tcaaaatcgt
     2821 aacgcctgag tctttgtccg tacacggcat gtacaaagtg tccgacaaag ccgacctgtt
     2881 cggcgacgta acttggacgc gccacagccg cttcaataag gcggaactgt tttttgaaaa
     2941 agaaaaaaat attgctaatg gcaaaaaatc cgaccgcacc accatcaccc ccaactggcg
     3001 caacacctac aaagtcggct tgggcggttc ttatcaaatc agcgaaccgc tgcaactgcg
     3061 cgtcggcatc gcttttgaca aaccgcctgt ccgcaacgcc gactaccgca tgaacagcct
     3121 gcccgacggc aaccgcatct ggttctccgc cggcatgaaa taccatatcg gcaaaaacca
     3181 cgtcgtcgat gccgcctaca cccacatcca catcaacgac accagctacc gcacggcgaa
     3241 ggcaagcggc aacgatgtgg acagcaaagg tgcgtcttgc gcacgtttca aaaaccacgc
     3301 cgacatcatc gacctgcaat acacctacaa attcaaataa acgttacacc gtttgaatat
     3361 aaaaatgccg tctgaaacag atttccgttt cagacggcat ttttatccgg cagccgatac
     3421 gcctatctcg gtattcggga gaatatatac agaacctcct tcatatcaaa atggtaggga
     3481 cgctcgggca acaggacggc ggcaggcagc ttggtgatgc cgttcaaacc gcgcgggaac
     3541 agcgcaatcc gctctttgcc cttttccgca taatctgctt tgcggttgtg gatggaaact
     3601 acttcgcgat ggcggtaaag cagcgtgttc atttcgatat aggcttcgtt gtggatggct
     3661 gccgactgtt tctgcaacac gtcaatctgc ctgttgagct tgtcgttggc tttcatcagc
     3721 ctgttgaaga acgacggttg ctcctgcagt ttggcggtac aggcttcgat actttccgat
     3781 accggcccga actgttcgag gtatttttcc agacagtttt ccaagtcggt caggcagccg
     3841 gccatcttgc gggaaaattc ttcttcaggc tttccctgcc gaatggacaa tatagcggat
     3901 taacaaaaat caggacaagg cgacaaagcc gcagacagta caaatagtac ggcaaggcaa
     3961 ggcgaggcaa cgccgtaccg gtttttgtta atccactata tttgggaggg gcgtaacccc
     4021 ttccgaatca ggatggcaca tagggcgacg ctttatgtgc cgtcctgtgt gttgaaacat
     4081 cagcatagga aacacaggaa aagccggctg ttttgcgcct gcgtaccgta ttggtcaaaa
     4141 tgccgtctga aaccgacaac agggtttcag acggcatttc cgtcaatttt ccctttatct
     4201 gccgaacagg ttggcgatat tttccatctg ttcttcggta aaaccgtttt cgcccaatct
     4261 ggcggcaact gcccattcgc ccgacaaacc gtggtccgca ccgagctgcc gccatttttc
     4321 gactttggct tcggctgtgg ttttttcggg cagcggtttg acgggaatcc tgcttttggg
     4381 gacggtattc atcggtttgc cctaatcgat ggaatagccg cgaagatact gtatccgttc
     4441 gcgcgtcatc cgcgtgtcgc attggagctt gaggtattcg gcgtattcct gccggtctgc
     4501 ctgcgcggcg gcttgtcggc agttgctgat tttttcctgc gcccacttgc gctgttcgcc
     4561 gaccaactct ttttgcacgt cggtatcgag tcctccccaa agtttggtaa tttcggattc
     4621 cgcacgttgg ttttgtacgc gcgcctcttc cacttcgccc cgtgatacgg taacggtatc
     4681 ggcacgctcg acgtcgtcgg gatgcaggat ttcgggttcg ggcgcgcctt ctgcggcttg
     4741 gggtacgccc gcatcgccgc cggcggcatt gtgttccaaa atgtcttcgg gggtgggttt
     4801 ggacggttct tcttcacggg ctttgccgct caaaaccctg accgcgtctt cttttgtcac
     4861 cgccttgccg tctatcatca cgatactctt cacgccgtaa ggcagcaacg cggcagacag
     4921 cgtttgcgtc gccataccga ccgtgttgtc gataaatgcc gtccgagcgt ctttggcggg
     4981 caggaagcgg acggctgccg tcaatacgcc gtctttaaac tcgacattgc cgcccgtctt
     5041 ctgctgcacg atgtctgcca aagacgtttc cccatacagc agggggctgt ttgcctcggc
     5101 atcggcaagc gtttcagacg gcacggtaat gttcaaatcg gcgatacaga acgtgcgccc
     5161 gccttcctgc gtttccgaag cgtgttccaa agaaaacgcc aaaccgtagg cggcggcgat
     5221 aattttgtcg gcatcgacaa actgcctgcc gtcttcgcgc gcgaaagaac gcgcttcctg
     5281 cgtgagcgtt tcctgaatac tgccgcgtat gtcctgcaac acggcggggt tggcgcattc
     5341 caacgccttg ggcggttctt ccctgccgca cgctgcaagc agcagggcaa acggcagcgc
     5401 aatgagtttc cgatacatga tgttccttcc aaacggtgca gaccgatggc ggctatcata
     5461 gcaaaagcac ctgcaggcgg taaaccttgc aggcgctttg tcggaaaggc ggaaaatcag
     5521 cctttaaaga aattcacaaa accggtgaga atcgcggcat taatcaaatc gacaaagaac
     5581 gcgccgacca taggcacaat caaaaacgcc ttatgcgacg cgccgaaagt atgcgtgacg
     5641 gactgcatat ttgccaccgc cgtcggcgtt gcgcccaaac cgaaaccgca gtggccggca
     5701 gccaatactg cggcatcata gtcgcgcccc ataaagacat aggtaacaaa agtcgcgtac
     5761 aaaaccatca ctgcggtttg tactgcaaga atcacggtta cagaccccgc caaaccggtc
     5821 agctcccaca gtttcaaatt cagcaacgcc attgccaaga aaagcgaaag cgaagcattg
     5881 ccgaacacat cgatggcacg gtcgaacata ttgaccttga atgcggcggt aaggatgttg
     5941 cggataacca cgccgccaaa cagacaccac acgaatttgg gcaggtcgaa caggtattct
     6001 ttgtcgaagc cgtccataat ctcggcaaac gccagacacg cggcaaacat ggcaagcgtt
     6061 tcaacggcag attccgccgt aatcaggcgg gtgcgttttg cctgctcgaa cacgtcgtcc
     6121 gcgttgtcgt cctgatcctg ttttgtgttt tcaaccggtt tgcggcccat tttgttgatc
     6181 aggcggcgcg caaccggccc gccgatcagg ccgccgaaca ccagcccgaa agtagccgaa
     6241 gcaataccca aaccggttgc gccgaccaag ccgtattgcg tttcaaaatt aggtccccac
     6301 gcacctgacg tgccgtgtcc gcccgtcagc gacaccgaac cggtaatcag accgatgagc
     6361 gggtccaaac ccaaagccgt agccagtccg accccgacaa agttttgcac caagataaat
     6421 ccgcccacaa tcgcggtaaa aaccaccagc ggcaaaccgc ccgccttcaa acgggaaaaa
     6481 tccgcgctca agccgatgga cgtgaagaaa atcagcataa acgcattttg cagcggtttc
     6541 tcaaatttga agctcacgcc gtacgcctcg tgcagcgcaa acaggataat cgcggcaatc
     6601 agcccgcccg ccacgggttc ggggatgtta aaatcacgca agattttgat tttcttaacc
     6661 aaaaccttgc cgaccaacaa aaccaaagtg gcggcaatca aggtgtaata actgttaaac
     6721 gcccattcca tatcgtttga cctcctaaaa tgtttttctt tatatccgcc cgaaggggac
     6781 gcaagcgcga agatattagg ggaatagtta aggcttgtca aagaatcgcc gttttttata
     6841 gtggattaaa tttaaaccag tacatcgttg actcgccttg ccgtactatc tgtactgtct
     6901 gtggcttcgt cgccttgtcc tgatttaaat ttaatccact atacaactat tttttacaaa
     6961 caaaatgccg tccgaaactc cgttcagacg gcatcatggg aatacacata tttgaaataa
     7021 cagaaaaagc gggggcaaaa cccgccgctg catctgtcgc tgtacatccc tgctgtcgga
     7081 cttaccgctt caacttcgca aacgcatccg ccatcgccga attggttggg acacgctcgt
     7141 tgcgttgtgg tttgcggccg gctgtccgtt cgcggctgcg gtttttccag ctcggacaga
     7201 atgtccagaa tggtcggcag tccgaagtgt tcgtcggtga agtcggacgc tttgatttgc
     7261 ttcacgcgct cgcggttaac tgcctaaata tgaaattctt ggtcttcttg tagcataagt
     7321 tctcgatgat atgccaagta agatttacgt ttgtcgttga gcagattgtt atctagcttc
     7381 acatcacacc aacgtcgcca aacatcatct gataaccgct tagatttcac catatttcct
     7441 tcatcatcaa acgaaatata tttcaaatca aataggctat ccaaggtacg acttaatagc
     7501 aagccgttat ttgggtcgta tgcttcggta tcatccgaca ggatgaaagg cttaatatga
     7561 ctggcaatca atacaggata ggcaagtttt tccagcatac atttcacatt accgtaatgt
     7621 tcttcacttt cttcttgaag ttggtttttg tataggcgat gtaaataagg gtcgcgtttc
     7681 cttacagtaa tttcatccaa attaccaaaa atacgttggg catcctcagc aaaatataag
     7741 tcatcaccta ctcgttttaa gtcgtctaat ttgtctaata ggttccataa ataacttatt
     7801 tgattgtatt ttcgttcaat aaatcctgat tctattccct gttgaaaata atcattcaat
     7861 tctgtttcgg ttagataatc ttgttgaaag gtttttaagt ctactaacat catggcggca
     7921 atttcttttt tatttagctt gccttgtgga tgctcgacca gcgttttaat caggaaattt
     7981 atttgtcgaa tattagagtt ttcagttact gaacgctgaa agccagagtg cgtatatacg
     8041 attttggaaa gtagggtttg tcgttttcgg ttagtacgag cttgtacata atctcgtgat
     8101 aaaggtgtat agcttgataa aaacggttct ataaatccca tttttacaaa ttgattaata
     8161 gctttacgaa ccgaggccaa attcatggcc ttgcttgttc tctgcgaagc cataacggaa
     8221 tcttgggcaa tgttttgctg caatgcttca tatagttcgt tgctgtatgg ctggtcttga
     8281 ttcgcatcaa taaagtcaag acaaatattg agtgcagtac aaaatttttg atcgttataa
     8341 tttgtaaagg cattggtaaa cgcccaataa tcttcataaa tttggctgtt tggctgatta
     8401 ttactcatct ctgtctccaa gaatattcga taccaatatt ctgtttttgt atttaagtaa
     8461 tatctatcat tcatgtattt tctactaaaa acagaatttc cttatggctt tgcttctttt
     8521 cttttcctgt aaccttataa acgtgaggcg tttccaagat ttgtacattt cgcccatttt
     8581 tacctaacat ttcttctaat tcatctcggt ttggatatga agcattgttg tagcttaaca
     8641 gccaatattt aaatggtttc aaatgattga ttaccctttt aaataattcg acagcctgct
     8701 ttttatccat gaaatgattg gcaaatggtt tgggtatgga tgatgtaatg taactgtcca
     8761 ataatccgta aaagccaaaa taattgttca tcgtgcctgt gtaaggtggg tctaaataga
     8821 cggcatcagc ttgtatatta ggtaataaat caaatatatc ttgattaaag gcgtgatgcc
     8881 gccagccatt attgaaaaca gcttggttat agtcatccaa attttgcaga aaatgacttt
     8941 gaaaactttc attgtggtag gcgcgtcgac ggccatattt agcataactg tattcttcat
     9001 cacgcagttg tttaactttt tcccaaggaa ttgtgaatcg tgaatagggc attttacgta
     9061 tcatggcgcg acgcattaaa gtaaacgcca aagcacgttt atactggtta ttcagcttcc
     9121 ctatattttt acggtacaaa tcaagttgtt ggcattcatc gtgaaaatag aatttttcgg
     9181 aataacgctg actcataaaa ccggcatgcg ggctgcctga aaaaatcatt gcgacatcat
     9241 cgtcatttaa tgtttcatgg ttgttttcta ttaatgctaa agcaatttgg taattaattg
     9301 ccaaaatatc gttagtaatc acacgatagc cgcgtttttt ggcttcgtag gcaaaggaac
     9361 agcctccact aaatacatct gcaactgtat ctacatcaga cggaagctgg tcacaaatcc
     9421 aggaagctat tttctcttta ttaccgatat agttaatttt cggatatttc ttattcattt
     9481 tcactcattt ttaaaatact ttcagcaata gcttttgcca ataaaggcgg cacggcgtta
     9541 ccaacctgct gctgctgggc aattttgctg ccgcaaaaaa taaaattatc agggaaagat
     9601 tgtaaggcag ctaattcacg aacggttaaa gcccgattct gttcatagtg aaaaactttg
     9661 cgcatatctc ctgtaataca aacggctggt ttgttgctgt tgtaacggat gtatttacgg
     9721 atatcacctg ttttcggacg taatggttca ggaatatcgt tacggttacc tccattttta
     9781 acaaatgcca ttttttctaa catttgtgcc gaatgattca tagcttcatg atttgcaacg
     9841 tgtggattgc tttcgccagc agccagtttt ggaaaatgtc ctattgctga tccaacagtc
     9901 tgatgggaaa tctgcaaagg ttcgggaaag gaaattttgc ctttatccct cctcccgata
     9961 aatatcactc ggctacgtat ctgaggaaca ccgaaatcgg ctgcactcag tatcttacat
    10021 tccaccgaat aaccgatatt ctgaaatgct tgaataatct caatacgtgt tttacctgaa
    10081 ttgtgtgtat agagtcgcgc tacattttcc ataacaaaaa aatatggttg gacaatttta
    10141 actattcgga caaactcttt aaataaatgg ttgcgtgggt catctgtaaa tgtccgtcca
    10201 atctttcctg ccatactaaa accttgacaa ggtggtcctc caataaccaa atcaactgat
    10261 tgtccgttaa gacaattggt taaatcttgt tcggttagtg tggttaaatc tttttgcagt
    10321 aattgatgac gggggaagtt ggtacggtaa gtctgacaat aatcagactc catttcaaca
    10381 gaaagcaatt gttggaatcc ggcttgttca aaacccaagg ataggcctcc tgctcctgag
    10441 aaaaggtcaa tataggtaag tggtgtgtgc ataagtcaaa atccataaac tcttcgatta
    10501 ttttaccgct tcaacttcgc aaacgcatcc gccatcgccg aattggtcgg ggcgcggtcg
    10561 ttgcgttggg gtttgcggcc ggctgtccgt tcgcggctgc ggttttcaga cggcatttta
    10621 tgttttgcgc cgcccggttc gtcatccaag cgcatggtca gcgcgatgcg tttgcgtgcg
    10681 gcatcgactt ccagcacttt cacttttacc acgtcgccgg ctttcaccac gtcgcgcggg
    10741 tcttggacga acttattaga cagggcggag atgtgcacca agccgtcctg atggacgccg
    10801 atgtccacga acgcgccgaa gttggcgacg ttggagacca cgccctcgag tatcatgccg
    10861 acttgcaagt cgctgatttc gtggatgcct tcggcaaacg atgccgtctg aaacgcaccg
    10921 cgcgggtcgc ggccgggttt ttccagctcg gacaggatgt ccaaaatagt cggcagaccg
    10981 aagcgttcgt cggtgaagtc ggatgctttg atttgcttca cacgctcgcg gttgccgatg
    11041 agttcggcgg cggtaatgcc ttgttgcgcc agcattttgg cgacgacggg ataggcttcg
    11101 gggtggacgg cgctcgcgtc cagcggctct ttgccgccgt taatccgcaa aaagcctgct
    11161 gcttgctcga aggttttttc gcccaagcgc ggtactttta gcaatttttt tcggctgtcg
    11221 aacgcgccgt tttcatcacg gtaggcgacg atgttttggg caagggtttg attcaagccg
    11281 gaaatccgcg ccaagagcgg ggcggaggcg gtgttcgcgt ccacgccgac ggcgttcacg
    11341 cagtcttcga ccaccgcgtc cagcgatttg gcgagccggc tttggttcac gtcgtgctga
    11401 tactgcccca cgccgatgga tttggggtcg attttgacca actcggcaag cgggtcttgc
    11461 agcctgcggg cgatggacac tgcgccgcgc agggaaacat ctaagtcggg aaactcgcgc
    11521 gccgccagtt cggacgcaga ataaatcgac gcgccggctt cggaaacgac gattttgtgc
    11581 aggctgcttt cgggcattcc gcgcaccagt tcgcccgcga ttttgtcggt ttcgcggctg
    11641 gcggtgccgt tgccgatggc gatgagcttc acaccgtgtt gtttaatcag gcgcgacaac
    11701 gttgccaaca tattgttttc ttgatgcaaa tagacaatga ccgtatccag cagtttgccc
    11761 gtgtcgtcca ccacggcgca tttcacgccg ttgcggtagc cggggtcgag tcccaaagtg
    11821 gtcagccgcc cggcgggcgc ggcgagcagc aagtctttga gattgcgggc gaacacggta
    11881 atcgcgtcag tgtcggcagc ttctttcaaa cgattgagag cttcgagttc cagcgacaaa
    11941 aagattttcg cgcgccaagt caggcgcacg gtgtcgcgca gccatttgtg gccgtctgaa
    12001 accttgaagc ggcgggcgat gatttgctcg tattcgcttt gctgcgtaat cggcgtgtcg
    12061 tcgggctggt atttgagcgc gatgttcaac acgccttcgt tgcgaccgcg caaaaccgcc
    12121 agcgcgcggt ggctgggcat ggcgcgcacg ggttcgcggc ggtcgaaata atcgctgaat
    12181 ttttcgcctt cggtttcttt gccttcaacg acttgcgtgt ggatttcggc ttcgttccac
    12241 agcttgtcgc gcagcgtgcc gatgagttcc gcgtcttcgg caaactgctc catcagaatc
    12301 gcgcgcgcgc catccagcgc ggctttggcg tcggggatgt tttcgttcag gtagccttgc
    12361 gcggcggctt ccacgtcctg cggctgttcg gcaagcaaca cgtccgccag cggctgcaaa
    12421 ccgtgttcgc gagcgatttg cgctttggtg cggcgtttgg gcttgtaggg cagatacagg
    12481 tcttccagcg cggttttgtt gtcggcggct tcgatttgcg ccctgaggtc gtctgaaagc
    12541 ttgccttgct cttcaatgct ttttaacaca acggccttgc gctcttccaa ctcgcgcagg
    12601 tattgcagcc gctcggcaag ctggcgcagc tgcgtatcgt ccagcccgcc cgtggcttct
    12661 ttacggtaac gcgcgataaa ggggacggtc gcgccgtcgt ccaaaagctc gatagcggca
    12721 ttgatttgca cggtagtggc ggagagttct tgggagagga ttcgggtaat gttcataaat
    12781 tgcctccgtg atgccgtctg aacagccgac ggcgcaatgt gggtaatcgt ttggaaaaga
    12841 tgaagataat actgatatac tttgcccgat acaatctgaa aggaatgttt atgaccgtcc
    12901 tgattaccgg cggcaccggc tttatcggtt cgcacaccgc cgtctcgctc gtccaatccg
    12961 gttacgatgc cgtgattttg gataatctgt gcaactcgtc tgccgccgtc ctcccgcgcc
    13021 ttcggcaaat taccggcaga aacataccgt tttatcaggg cgacatccgc gactgtcaga
    13081 ttttgaggca gattttttca gaacatgaaa tcgaatccgt catccatttt gccggtttga
    13141 aggcagtggg ggaaagcgtt gccgagccga caaaatatta cggcaacaat gtttacggca
    13201 gcctggtgct ggcggaagaa atggcgcgcg cgggcgtgtt gaaaatcgta ttcagctcgt
    13261 cggcaaccgt ttacggcgat gcggaaaaag tcccctatac ggaagatatg cgcccgggcg
    13321 ataccgctaa tccttacggt gcgtccaaag cgatggtgga gcggatgtta accgacatcc
    13381 aaaaagccga tccgcgttgg agcgtgattt tgttgcgcta tttcaacccg atcggcgcgc
    13441 acgaaagcgg acttatcggc gaacagccca acggcgttcc caacaatctt ttgccctata
    13501 tctgtcaagt ggcttcgggc aggctgccgc aactgtcggt attcggcggc gactatccga
    13561 cccccgacgg tacgggaatg cgcgactaca tccatgtgat ggatttggca gaagggcata
    13621 tcgcggcaat gaaggcgaaa ggcggcgttg ccggcgtaca tttgttcaac ttgggttcgg
    13681 gacgcgccta ttccgttttg gaaatcatcc gcgcctttga ggccgcatcc ggtttgcaca
    13741 ttccttaccg aatccaaccc cgccgcgccg gcgacttggc gtgttcctat gccgacccgt
    13801 cccataccaa acaacaaacc ggctgggaaa ccaaacgcgg cttgcagcaa atgatggaag
    13861 attcgtggcg ttgggtcagc cgcaaccccg gcagatatgg ggattaaatg caaaccgcag
    13921 gcaaaaaaaa catcctcgtt accggcggcg cgggttttat cggctcggca gtcgtccgcc
    13981 atattatcca aaacacccga gattccgtcg tcaacctcga caagttaacc tacgcgggca
    14041 atctcgaatc gctgaccgac attgccgata atccccgcta cgcttttgag caagtggata
    14101 tttgcgaccg cgccgaactc gaccgcgtgt tcgcgcaata ccggcccgat gccgtgatgc
    14161 acttggcggc ggaaagccac gtcgaccgcg ccatcggttc ggcaggcgaa tttatccgaa
    14221 ccaatatcgt cggcacattc gacctgctgg aagctgcgcg cgcctattgg cagcaaatgc
    14281 cgtctgaaaa acgcgaagcc ttccgtttcc accatatttc taccgatgaa gtctatggcg
    14341 atttgcacgg cacagacgat ttgtttacag aaaccacgcc atacgcgccg tccagcccct
    14401 actccgcctc caaagccgcc gccgaccacc ttgtccgcgc gtggcagcgg acttacaggc
    14461 tgccctccat tgtcagcaac tgttccaata attacggccc ccgacaattc cccgaaaaac
    14521 tcattccttt gatgattctg aacgcacttt ccggcaaacc actgcctgtg tacggcgacg
    14581 gggcgcaaat ccgcgactgg ctgtttgtcg aagaccacgc gcgcgcgctg tatcaggtcg
    14641 ttaccgaagg tgttgtcggc gaaacctaca atatcggcgg acacaatgaa aaaaccaatc
    14701 tcgaagtgat caaaaccatc tgcgccctct tggaagaact cgcccccgaa aaaccggccg
    14761 gcgtggcacg gtatgaagat ttaattactt tcgtacaaga ccgccccggc catgatgccc
    14821 gctacgccgt cgatgcggcg aaaatcaggc gggatttggg ctggctgcct ttggaaactt
    14881 ttgaatccgg cctccgcaaa accgtgcaat ggtatttgga caacaaaacc cggcggcaaa
    14941 acgcataaaa cggcaactgc cgttcggaac gcttaggtac gggcaaatag ttttcagacg
    15001 gcatcccgac gcaatgccgt ctgaaaaccc atcgcaaagg aagaaggaaa agatgaaagg
    15061 catcatactg gcaggcggca gcggtacgcg cctatatccg attacgcgcg gcgtatccaa
    15121 acaactgctg cccgtgtacg acaaaccgat gatttattac cccttgtcgg ttttgatgct
    15181 ggcgggaatc cgcgatattt tggtaattac cgcacccgaa gacaacgctg ccttccaacg
    15241 gctactcggc gacggctccg acttcggcat ccgcctgcaa tatgccgttc aacccagccc
    15301 cgacggcttg gcgcaggcat ttatcatcgg cgaagaattt atcggcaacg gcaatgtctg
    15361 cctgattttg ggcgacaata ttttctacgg tcaatcattt acgcaaacat tgaaacaggc
    15421 cgccgccaag acccacggcg caaccgtatt cggttatcgg gttaaagacc ccgagcgttt
    15481 cggcgtggtt gaatttgacg aaaacttcaa cgccttatcc atcgaagaga aaccgcaaca
    15541 gcccaaatcc gattgggcgg taaccggctt gtacttccac gacaaccgcg ccgtcgagtt
    15601 cgccaaacag ctcaaaccgt ccgcacgcgg cgaattggaa atttccgacc tcaaccggat
    15661 gtatctagaa gacggctcgc tgtccgttca aatattggga cgcggtttcg cgtggttgga
    15721 caccggcacg caagaaagcc tgcacgaggc ggcttccttc gtccaaaccg tgcaaaatat
    15781 ccaaaacctg cacatcgcct gcctcgaaga aatcgcctgg cgtaacggct ggctgacgaa
    15841 aaaagatgtg gaaacacggg caaagccttt ggaaaaaacc gcctacgggc aatacctgct
    15901 gcgcctgatc ggcaaataaa taccgtctga acgccccgac ggctttcaga cggcatccaa
    15961 accgtgccgc gccgccacct taaccgacct gccgcacggc gcgcataaaa aatcaaaccg
    16021 caaaaaacag cggtaacgat accgccaaag gactttatgg acatcatcga caccgccctc
    16081 cccgacgtaa aacttttaaa accgcaagtc ttcacagacg ggcgcggttt ttttatggaa
    16141 acattccgcg acggatggtt caaagaaaat attgccgatc gaactttcgt gcaggaaaac
    16201 cactccaact ccagcaaggg cgtattgcgc ggcctgcact accaaaccga aaacacccaa
    16261 ggcaaactcg tacgcatagt tgtcggcgaa gtgttcgacg tggccgtcga tatgcgcgaa
    16321 ggttcgccga ctttcggcaa atgggcgggc gcaaccttgt cggcgcaaaa ccgataccag
    16381 ctttggatac ccgaaggctt cgcacacggt ttttgcgttt tgggcgacgc ggcggaagtc
    16441 gtgtacaaat gcacggacta ttacaaccct gaaaccgaac aggttttaat atggaacgac
    16501 ccggcaatcg gcataggctg gccgcttcaa accgcgccgc tactttcgcc caaagacctt
    16561 gccggcaaaa cgtgggcaca agccgaaaag ctccgcctta cgctttcccg ataaaaaatg
    16621 ccgtccgaac gtttcagacg gcattttctc cgacaggcta cctacccgcc ttcagtacgc
    16681 gctgtgcaaa gaaaaacatc ccggtaacga agaacgccaa gcccaaccaa gaagcggcgg
    16741 catcccagtt ttgcagattc aacgcgagcg gcgcatccga gccgccgttg gtcacagaga
    16801 aggcaataat aaacgccata attacaccaa tcaggctttc accgacaatc aggcctgccg
    16861 cgaaaagcgt accgatacgg tcggcgtttt tcagacggcc ttcgcggttt tccgccttct
    16921 tgccaatgat gtgtttcaac accgccgcca acaccgcgcc tgccacgatg ggcatattga
    16981 cggacggcgg cagataaatg cccataccga ccgcaaggac gggcagggca agtttaccgc
    17041 ctgatgattt tttcaacacc aaatcgacga cgattaatac cgctccaatc gcgataccgg
    17101 taaagatgta cgcccattcg aggttgtggg cgaaaatgcc cgacgcaatg gtcgtcatca
    17161 aagtcgcttg aagggctgcc aaagcctgcg ccgcatccat gccttcgcgc ggcattgcgc
    17221 cggtaaagcc gtaggcttcg taaagcagtt ccaacacggg cgaaataacc agcgcaccaa
    17281 cgatacagcc gataatcagg gcgacttgct gccgccaagg cgtagctttg agcaggtagc
    17341 cggttttcaa gtcttgcagg ttgtcgttgg aaatcgaagc cacgcagatt actgccgatc
    17401 cgcaaaaaag cgtcaacgcc agtaagaatt tgcggttggc ctcatccgcc aacaaacctc
    17461 cggattcgcc taccagcagc aaaaccagtg aaataacgac gatggacacg atgcccacgc
    17521 cggaaatcgg gctggaagac gagccgacca aacctgccat ataaccgcag gcggcggcga
    17581 ccaaaaagcc gatgacggaa gccaaaagcg tgcaaaccgc caccaaaagc cacgccatgc
    17641 cgcccgtaat gtgcgaatcg ccgataaagt ggtaaaacga cacgcctaga ataaacatca
    17701 tagacaacac ccagaaaatc atggctttag gcgacaaatc ctgttcggcg cgttctgtag
    17761 cgggcgcacc gccgccaaaa ctcttgaacg acatcttcat gccttccacc attggcttga
    17821 gcagcatcaa aagcgtccaa accgccgcga tacctatggt tcccgcaccg ataaaacgca
    17881 ctttttcctt ccacagcttc atcgcaaacg ccgccatttc catatcggaa ggttgcggaa
    17941 tgtgtgagga gaaatacggc acggcaatac cccaagcaat cgaaatgccc agcaggatgg
    18001 cgatgccgcc cgtcagtccg accaaataac ccgcgcccaa caaggccagt gaaaagccca
    18061 tcggcagttg gaaaattgcc gtaccgcttt taaaccaata actcgcgcta tcggcaatca
    18121 cacgcagacc tccggcgcaa aagctcatca atcccgccaa cgcgccgccg gccgccagct
    18181 ctttgatacc gctgccgccc tgacggtcat ccccttcttc atgaccgccc actttcaaaa
    18241 tttcagcagc cgccacacct tccggataag gcaaatcgct tttcaccacc atcgcgtaac
    18301 gcagcggaat ggtgaaaatc acccccaaaa tcccgccggc aatacataaa agcgtcgtct
    18361 gccaaaacgg gaaaccgctc cagtagcccg ccatcagcaa accgggcagg acgaagatga
    18421 tggttgaaag cgtacccgca gccgaggctt gggtctgcac catgttgttt tccaaaatat
    18481 tgctgccttt gaaaaatttt aaaaccgcca tcgaaatcac cgccgccgga atcgacgagg
    18541 caaaggtcag cccgactttc aaaccgaggt aaacgttaga cgcagtaaaa attacagtga
    18601 tcaatgcgcc gagtatcatg cctcggagcg tcagttcgcg gtattcttct accgaaccgg
    18661 ataaagattt attcattatt cttcctttga caacagacgt tcacatattg ttggcatcac
    18721 gccatgatgt caagttttaa aaagaacagt taaaaacaat tatcccaccc tgcccatacc
    18781 caattgaaaa taaaaactat ttccaaacga acaaaaacag ccgtatcagg gaggttcccc
    18841 gatacggctg cttgtttccg aaccttaaaa tcaatcaaac aaatcgcgca gcttgtctaa
    18901 aaacgatttc ttgcgcggcg tttggttttc caagcccgta gaaatccgct caaattcttc
    18961 caaaagctct ttttgacggt cggtcaaatt gacaggcgtt tcgacaacaa tatggcagta
    19021 caaatcgccg gtcgcgctgc tgcgtaaaga tttgacaccc ttacctttca cgcgcatcct
    19081 cctgccggtt tgggtttctt tggggacggt gagcttgact ttgccgtcca aggtcggcac
    19141 ttctaactcc ccgcccaaag cagccgtggc aaaactaatc ggcagttcgc aatgcaaatc
    19201 caaaccatcg cgttggaaaa tcttatgcgc ccgaatgcgg acggttacat acaggtcgcc
    19261 ggcaggcgcg ccgtgcatac ccggcccgcc ttcaccgctc aaacggatac gctgcccgtc
    19321 atcgataccg gcgggaatat tgacttccac cgtcttgacc gccttattcc gccccacgcc
    19381 acggcatttg acgcaaggtt ctttaatatg tttgcccgca ccgcggcagg tcggacaagt
    19441 ctgctgcata cggaaaatcg cctgctggat gtgcaccgta cccgaacctt tgcaagtcgg
    19501 gcaggtttcc ggggatgccc ccggtttcgc gccgctgccg ttgcagacat cacacgcttc
    19561 ataagtcgga atattgatgc gtttcttcac accttttgcg gcttcttcaa gcgtgatttc
    19621 gataccgacc tgcacgtcct caccctgata atcaggctgg gcgcgccccg aaccacctcc
    19681 aaacatttgg ctgaaaatat ccccaaagtc aaaaccctgc gcaccgccaa atccgccaaa
    19741 ccctccgaag cccccctgtc cgccgccttc aaacgccgca tgaccatact ggtcgtacat
    19801 ggcacgcttt tccttgtcgg acaaagtttc atacgccttt tgtacttctt taaacttctc
    19861 ttccgcctct ttattgtcag gattgcggtc gggatggtat ttcatcgcca atttacggta
    19921 ggctttttta atctcatcat cggtagctgc tcttgccaca cccagcgtcg cataaaaatc
    19981 ttgattactc attttttcat ctaaaaataa aatcacggct taaaatgagg gcaattgcgc
    20041 aaaacacaag acaaacagac tgagaccttt gcaaaa
//
LOCUS       DAXDSD010000033        19617 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXDSD010000033 DAXDSD010000000
VERSION     DAXDSD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19617
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.033"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(2..466)
                     /locus_tag="ACFB2Q_000916"
     CDS             complement(2..466)
                     /locus_tag="ACFB2Q_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917965.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGEGKWRFANNALTQSQIKYGEVSRQHSPETLAWLE
                     KSEDGRLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(543..3380)
                     /locus_tag="ACFB2Q_000917"
     CDS             complement(543..3380)
                     /locus_tag="ACFB2Q_000917"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC0917966.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFRPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDGVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(3485..4744)
                     /locus_tag="ACFB2Q_000918"
     CDS             complement(3485..4744)
                     /locus_tag="ACFB2Q_000918"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC0917967.1"
                     /translation="MKVLVLGAGVAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVETETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(4823..6214)
                     /locus_tag="ACFB2Q_000919"
     CDS             complement(4823..6214)
                     /locus_tag="ACFB2Q_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC0917968.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGTGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            6414..6560
                     /locus_tag="ACFB2Q_000920"
     CDS             6414..6560
                     /locus_tag="ACFB2Q_000920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917969.1"
                     /translation="MGMWRAAEIINAADCHTSEIYSGLNLNRYSVASPCRTSCTVCGF
                     VALS"
     gene            complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="ACFB2Q_000921"
     CDS             complement(6677..7453)
                     /gene="lpxA"
                     /locus_tag="ACFB2Q_000921"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC0917970.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVRIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(7496..8176)
                     /locus_tag="ACFB2Q_000922"
     CDS             complement(7496..8176)
                     /locus_tag="ACFB2Q_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC0917971.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIYQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="ACFB2Q_000923"
     CDS             complement(8383..8832)
                     /gene="fabZ"
                     /locus_tag="ACFB2Q_000923"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC0917972.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="ACFB2Q_000924"
     CDS             complement(8868..9911)
                     /gene="lpxD"
                     /locus_tag="ACFB2Q_000924"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC0917973.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTIVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHIAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(9944..10444)
                     /locus_tag="ACFB2Q_000925"
     CDS             complement(9944..10444)
                     /locus_tag="ACFB2Q_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC0917974.1"
                     /translation="MTRLTRAFAATLIGLCCTTGTHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="ACFB2Q_000926"
     CDS             complement(10510..12888)
                     /gene="bamA"
                     /locus_tag="ACFB2Q_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC0917975.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK
                     GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="ACFB2Q_000927"
     CDS             complement(12945..14285)
                     /gene="rseP"
                     /locus_tag="ACFB2Q_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC0917976.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(14320..15555)
                     /gene="ispC"
                     /locus_tag="ACFB2Q_000928"
     CDS             complement(14320..15555)
                     /gene="ispC"
                     /locus_tag="ACFB2Q_000928"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC0917977.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15560..16357)
                     /locus_tag="ACFB2Q_000929"
     CDS             complement(15560..16357)
                     /locus_tag="ACFB2Q_000929"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC0917978.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(16360..17106)
                     /locus_tag="ACFB2Q_000930"
     CDS             complement(16360..17106)
                     /locus_tag="ACFB2Q_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC0917979.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(17162..17719)
                     /gene="frr"
                     /locus_tag="ACFB2Q_000931"
     CDS             complement(17162..17719)
                     /gene="frr"
                     /locus_tag="ACFB2Q_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC0917980.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(17861..18853)
                     /locus_tag="ACFB2Q_000932"
     CDS             complement(17861..18853)
                     /locus_tag="ACFB2Q_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC0917981.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDGCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(18858..19262)
                     /locus_tag="ACFB2Q_000933"
     CDS             complement(18858..19262)
                     /locus_tag="ACFB2Q_000933"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC0917982.1"
                     /translation="MVYGIDYCADAECYLKIKNQIKEGIGNIGGIQFAETKELGRVNR
                     IDPLNITYLRVRGMWGIENPWFVFNYIYQRNMEKSFNFMAIINEDKWNSFNNTDKLLA
                     IQDSKLAISDIKIKNPNNPARLRNAKLITYHL"
     gene            complement(19291..>19617)
                     /locus_tag="ACFB2Q_000934"
     CDS             complement(19291..>19617)
                     /locus_tag="ACFB2Q_000934"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFC0917983.1"
                     /translation="RANQAGDALEEYVKDLFSGSFNLNETQRIARHAKVFSYLGNNSN
                     PPDAMLRNGDAIEVKKIESKDSALALNSSHPKSKLSVDDSMLTKACKDAEKWEEKDII
                     YNCRRR"
BASE COUNT         4017 a         4987 c         5533 g         5080 t
ORIGIN      
        1 ttcactccac tttcgtacat gcctcacggg aagttccgtc ttcggacact aatttgcctg
       61 atttatccag ttgatacaca tcatcttttc cgggtttgtc catctgcgcg accaatccgg
      121 tgtacatcat gctttccaag agtcgtccat cctccgactt ttctaaccac gccagcgttt
      181 cggggctgtg ctgtcgtgaa acttcaccgt acttgatttg gctttgagtt aaggcattgt
      241 tcgcaaaacg ccatttacct tcaccgacaa cacggtatgc caagatattg ccgtcatcat
      301 cgattttgag tatagcatcg ccacggaagc tgccgtcctg aagatatttg acttttgtat
      361 cactgtgaat gttttcatca gtgccgatgc aatgccacgt accgacaatc ggagaagcca
      421 aggcggttgt tgcggataaa agcaccggca gaataaagcg tttcatctat ttcctttttt
      481 aaaggaggcc gtctgaaaac cccggtttca ggttttcaga cggccttgtt tattcaatca
      541 aatcagtctt tcaacttcgc caactgattt tgcacttttg ccattttgtc ttccaattcc
      601 gccaaatcgg ctttgtcttt ttccaccaga tgcgccgggg ctttttcggt gtagccgggt
      661 ttggagagtt tggcattgag tttgtccaag gctttttgca gcttctcggc ttctttgctc
      721 aaacgggcgg tttcggcggc tttgtcgatt tcgactttca gcatcaggcg cgcgccgttg
      781 cagacggcga cgggcgcgtc tccgctttcg ggcagggcgg cgacttgccg tgcttcggtc
      841 aggcgggtca tcatcggcag gtatttgagg tagtccgcca agtcgtccgc gctttcgaca
      901 aacagcgggg ctttcacgtt gggctggatg cccgtttcgc cgcgcaggtt gcggactgcg
      961 ccgatcaaat cctgcaacac ggtcatttgc ccgaatgccg tctgaacaat ctcgccgccg
     1021 tcggtttcgg ggaagcgggc gagcatgatg ctgtcggcgg ttttggcatc gcacatgggg
     1081 gcgacggttt gccacagttc ttcggtgatg aacgggataa tcgggtgcag caggcgcagg
     1141 gcggcttcga gtacgcgcaa taaggtatgg cgtgtggcgc gctggcggct ggcgcagccg
     1201 gtttggagct gcactttggc gagttccaga taccagtcgc aataatcgtt ccatacgaag
     1261 ctgtacaggg tttccgccgc caaatcaaag cggtaggttt cgtaggcttg cgtaacctgc
     1321 tcgatggtct gattcagacg acctatgatc cacatatcgg ggaaggaatg gccgcgcggt
     1381 tcggcagcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa gacgaagttg
     1441 gtggcgttcc agattttgtt gcagaagttg cggtagcctt cggcgcgttt gaagtcgaag
     1501 ttaaccgaac ggcccaagct ggcgtagctc gccatggtga agcgcaatgc gtccgcgccc
     1561 atgctgggga taccttcggg gaagagtttt ttcgtggctt cttccacttt cggcgcggtt
     1621 tcgggtttgc gcaggccggt cgtacgtttc atcagcagct tgtccaagcc gataccatcg
     1681 atcaaatcca cagggtcgat gacgttgcct tcggatttgg acattttttt gccttcgtgg
     1741 tcgcgcacga tgccgtggat gtacacggct ttaaacggta ctttgccggt gaagtgggtg
     1801 gtcatcataa tcatgcgcgc cacccagaag aagatgattt catatccggt aaccaagacg
     1861 ttggacggca ggaaggcttt gagctcgtcg gtttcagacg gccagccgag cgtggagaac
     1921 ggcacgagcg cggaggagaa ccatgtgtcc aatacgtctt cttcgcgggt caaacctgtt
     1981 ttgccggctt gtttttcggc ttcagcctga ttgcgggcga cgtaaacatt acctgcttcg
     2041 tcgtaccacg cggggatttg atgcccccac cacagttggc gcgaaataca ccagtcttgg
     2101 atgttgttca tccattggtt gtaggtattg acccagtttt cagggataaa gcgcactgcg
     2161 ccgctgtcga cggctttttt ggctttgtcg gcaaggctca agcctttgaa ttcgttgtca
     2221 ggctcgccgc cgttcggtgt ggcggacatg gcgacaaacc attggctggt cagcatcggc
     2281 tcaatcactg aacctgtgcg gtcgcctttc ggcgtcatca gcgtgtgcgg tttgatttcg
     2341 accaagaagc cttgctcctg caaatcggca accatttgtt tgcgcgcggc aaagcggtcc
     2401 aagcctgcgt atttttcagg cagggaaaag cctggttgcg cttcgccttt gaagttgaac
     2461 acttcggcgt ttgccagcac tttggcttct aagtcgaaca cgttaatcag gcgcgtgtcg
     2521 tggcgtttgc cgacttcgta atcgttgaaa tcgtgcgcag gcgtgatttt cacgcaaccg
     2581 gtaccgaagt ctttttcaac gtattcgtcg gcgataaccg gaatggtgcg accggtcagc
     2641 ggcaggatta attctttgcc gattaagtgg gtgtaacgtt catcttctgg attgacggca
     2701 acggccgcgt cgcccagcaa cgtttcagga cgggtggtcg ccacgataac ggcttcggtg
     2761 ggattgtcgg ccagcggata gcggatgtgc cacatagagc cttgttcttc catgctttcc
     2821 acttccaaat ccgataccgc cgtgccaagc acgggatccc agttcaccaa gcgtttgccg
     2881 cggtaaatca agccctgttc atacaggcgc acgaacactt cggtcacggt ttcggcgcgt
     2941 acgccgtcca tcgtgaaata ctcgcgcgtc cagtcggcgg agcagcccac gcggcgcatt
     3001 tgctgggtaa tcgtgccgcc ggaaacttct ttccattccc acactttttc caagaatttt
     3061 tcgcggccca agtcatgacg ggacacgttt tgcgcggcaa gctgacgctc gaccacgatt
     3121 tgcgtggcga tgcccgcgtg gtcggtaccg ggaatccagg cggtgttgca gcctttcatg
     3181 cggtagtagc gggtcaggcc gtccatgatg gtttggttga aggcatgacc catgtgcagc
     3241 gtgccggtta cgttgggcgg cggcagttgg atggaaaaag acggtttggt caaatccata
     3301 tcgggccgga aatagccttg gctttcccag ttttgatagt gtttggattc gatttcggcg
     3361 ggggagtatt tgtctaacat atgaagccct gagaaataag atgatttgat ggtttgaatt
     3421 atagcgcaaa tgccgtctga aacgctttcg gggtttcaga cggccttaaa tttaaatatt
     3481 gcctttacag tttttgatag cgcgacaggc tcaaatcgtc gctgcggatt tcggtgtctt
     3541 tgccgctcac gatatcggcg gtcaattttg ccgaacccgg cgacatggtc cagcccaaag
     3601 taccgtgccc ggtattcaga aacaggtttt caaagcgggt gcgcccgatt aacggcgtac
     3661 tgtcgggcgt catcggcctg aggccgctcc aggacaatgc ttggctcaaa tcgccgcctt
     3721 ccgggaacaa gtcgttgacg accaaagcca aggtttcgcg gcgtttttcg ggcagtttgg
     3781 tttcgtagcc ggacaattcc gccattccgc cgacgcggat tctgttgtca aaacgcgtga
     3841 tggcgacttt gtagctttca tctaaaacag tggacaccgg tgcgccgtcg gaatttgtga
     3901 ccggcagggt caaggaatag cctttgacgg gataaatggg cagattgaga tccaactgcg
     3961 ccaacacagt cctgctgaag cagccgagcg cgcagacaac ggcatctgtt tcaaaccgcc
     4021 ctgtttcggt ttcaacggct ttgatgcgca gcccgttgtg gtcgatgcgg ctgatggttt
     4081 ggttgaagta gaaccgtacg cccttctctt gacacaattt gtacaggttt tcggtaaaga
     4141 ggcggcagtc gccggtcgca tccgcaggca ggtgcagacc gccgacaatt ttggcggtaa
     4201 cgcgtgccag cgcaggctcg aattctgcgc attcttcggg cttcagacgg cggtacggca
     4261 cgccgtagcg ttccaaaacg gcaatgtctt gttttgccgc ttcgacttct tcggtttggc
     4321 ggaaaatctg caacgtccct tttttgcgtc cctcaaaatt catgtcggtt tgcgcttcaa
     4381 aacggcggaa catttcacgg ctgtattcgg aaatcctgac catgcgctct ttattgattt
     4441 gatagcgcgt tgccgtgcag ttttgcagca tccgccacag ccattcgatt tgatacaggc
     4501 cgccgtcagg gcggaacagt aaaggcggat ggcttttaaa cagccgtttc agtgctttgg
     4561 tcggaatacc gggtgcagcc caaggcgtgg tatagccgta agaaagctgg cctgcattgg
     4621 caaaactggt ttccatcgcc acaccctcgg tgcggtcgat gaccgttact tcatgtccgg
     4681 cctctgccag ataccacacg gaggatacgc cggcaacacc cgcacctaaa acaagcactt
     4741 tcatgtttcc ccctccggct ttttcaaaac aggcttaata tgccgtgccg tctgaatatt
     4801 cggattcaga cggtctcgga tattaatgcg gcaattcgcc gtttgtgatt ttttgtttga
     4861 agtcgcgtgt ttcattgacg atgactttcg ccatcaacaa aagcgcagtc aggttgggca
     4921 atgccatcaa gccgttgaat gtatccgaag ccagccatac caaatcaagg ctcaacacgg
     4981 tacccggcat gacggaagaa acataaccga cgcggtacaa agcggcaaac ttctcgccga
     5041 agacgtaaac cgcgcatttc tcgccgtaat aacaccagcc gagaatggtt gagtaggcaa
     5101 agaaaatcag gccgatggtc acaatccagc cgccgatgcc gggcagcatt ttttggaaag
     5161 tgacggttgt cagcgccgcg ccgctcaatt caggtttcac aaactcgccg cctgcgccga
     5221 gcagacccat gaccaacacg ataccggtaa tcgagcagac aacaatggtg tccaaaaaag
     5281 taccggtcat ggaaaccaaa gcctgacgaa cggggtggtc ggttttcgcg gctgcggcgg
     5341 caataggcgc agaacccata cccgcctcat tggagaacac gccgcgcgcc acgccgtagc
     5401 ggatgaccgt accgatagcg ccgcctgcta cggcctgcgc gctgaacgca tcggagaaaa
     5461 tcagcttgac ggcaggcatc agtgcatcgg aattaatgac gataatggaa agaccgccca
     5521 acacataaaa caccgccata gcaggcacga tgaaagaagc ggctttggcg atgcctttaa
     5581 taccacctaa aacgacaacg gcagtcagaa cggtcaacgt aatgccggta taggcaggtt
     5641 cgataccgaa gctggtttgc acagcctgtg caaccgagtt ggactgcacc gagctgccga
     5701 taccgaagga agcgaatgtg ccgaacagcg caaatgcaac ggccatccat ttccagtttt
     5761 tgcccaagcc tttttcgatg taatacatcg ggccgccgga catttcgcct ttggaattgt
     5821 tgacgcggta tttcaccgcc aacacgcctt cgccgtattt ggtggccatg ccgaaaatag
     5881 cggtcatcca catccagaat accgcgcccg ggccgccggt taccactgca gtcgccacgc
     5941 cggcgatgtt acccgtaccg atggtggcgg acagcgcggt catcaacgcc gcaaaatggg
     6001 aaatatcgcc ttcgtggcct gtgccgcctt catacttctt tgacggcaca aacgcctgtt
     6061 tcagcgcata gcccaacata gtgaactgca agcctttcag cataacggtc agcacaatgc
     6121 cggtgccgac cagcagcatc aacataacag atccccaaac ccagctgctg acggtttcga
     6181 aaaaggcttt gagattgtct aaaaatactt gcatggcttt ctcctttgtc tgttttattt
     6241 ttaaaacacc acttttgtag tgtccggtaa tttcagcaca gaatattcaa taagacaata
     6301 tgttcttttg aaaaatactt ttggtttttt caccgaagac aggccggttc aagctgcgga
     6361 aattgtttgc aattatttaa aagcagaggc ggaggtcaca atgaaatgtc cgaatgggga
     6421 tgtggcgggc ggcagaaatc atcaatgctg ccgactgcca tacttctgaa atctatagtg
     6481 gtttaaattt aaaccggtac agcgttgcct cgccttgccg tacttcttgt actgtctgcg
     6541 gcttcgtcgc cttgtcctga tttaaattta aaccactata caaaatgatg catcgatcaa
     6601 acaacatacc gctttaaaaa aaaccgatgc cgtctgaaac gctttcgggg tttcagacgg
     6661 cattgaaagg gtgcggtcag cggatgatgc cgcgcgtcga ttgtgcgaag aagtcttgaa
     6721 atacggcaag ctcggcttgg gtttcggcgc ggcggagaat gtccgccttg gcttcttcaa
     6781 acggaatgcc gcgatggtag agggttttgt atacgtcttt gacggcggaa atctgttctg
     6841 cggtaaaacc gttgcggcgc ataccttcgc tgtttaggcc ggccggttcg gcgcggtagc
     6901 ccgatgccat aaagtagggc ggcacgtctt tgtgtacgcc tgcggcgaag gcggtcatgg
     6961 cgtagtcgcc gatgcggcag aattggaaga ccagcgtgta gccgcccaaa acgacgtagt
     7021 cgccgacggt aacgtgtccg gcaagcgagg cgttgttggc gaaaatggtg tggttgccga
     7081 ccacgcagtc gtgcgcgagg tggcagtacg ccataatcca gttgtcgtcg ccgatacggg
     7141 tttcgccgat gccggttacc gtgcctaaat taaaggtggt gaattcgcgg atggtgttgc
     7201 cgttgccgat aatcagcttg gtcggctcgt cgcggtattt tttgtcctgc ggaatttcgc
     7261 cgaggctggc aaattgaaaa atgcggttgt tttcgccgat ggtggtgtgg ccgttgatga
     7321 cggcgtgcgg accgatttct gtattcgcac cgattcggac gttggggccg ataacggtgt
     7381 acgcgccgac tttgacgccg gagtcgagtt cggctttggg gtcgatgacg gcggtcgggt
     7441 ggatgagggt catgttttga atactccgct gcttgttaag acaaataata gaaaatcaca
     7501 gtttgtcgat ttcatcgcag accgcaatca cttgatcgct aaaatggcta aatactgaag
     7561 cagtatctac atccatgtaa ttaagcagtt caaaaataga ttggtaaata tttctagcgt
     7621 agccggtatc ttttattgca cctttaaaac aaatcggttc gtgatgtgcc aaacgattac
     7681 ggaaattgtt aatattggaa agttctcgaa aaatccattt ttggttatat tgcacacttg
     7741 gtgtagattt gggctttttt ggaaaaactt tcatcaatac ttttcctgca gcatcaaatt
     7801 gagcatcttt ccctcccgca aacagatacc gccaaaaacc gaatcctagt cctgcaacca
     7861 attggttgtg gtcaaacttt cctccgctat tattttgtat tttagataat gcaaccttga
     7921 ttaattcagc agattctttc gtaccatttc ttaaaaagca accttgatat ttcaatgcag
     7981 gattcgtttg tggttgtatg ctatcatata accggtttct gtctttaaac tcctgccgaa
     8041 agcaaatatc aattttgttt cttaaaacga tctcaaatat agaaacaaca tgaaaaagtt
     8101 ctcttgataa agataaattc aataaataca attcagctgc tttgtctttc ctaacggcat
     8161 ttttataccg ttccattctg aaatcagaaa aaatactaca gcattcctta taattcacgc
     8221 taacccttga ttctttattt ttaaattgac gtttcaaact atatttgata tagttagata
     8281 aaactaatcc ccaccgtgtc cctgggtttg aataaaccta aaccggtggg ttttcttttt
     8341 attaggtcgt ctgaaacttt cagacgacct ttttccgaac gctcaaacca cgcgtttggc
     8401 gcacataatc acggcttcga cggcgacttg tccgtccact ttggcaacgg cgttgaattt
     8461 gccgatgccg cgccggctgg tcaggagttc gacttcaaag acgagttggt cgccggggat
     8521 gacttggcgt ttgaaacggg cttcgtctat gccggcgaag aagaaaaatt cgttttcctt
     8581 gcgcccgcct tcgctcaaaa tcgccaacgt gccgcacgcc tgcgccatcg cttcgatgat
     8641 gagtacgccg ggcataacgg gcaggtcggg gaaatggcct tggaattggg gttcgtttat
     8701 ggttacgttt ttgatggcgg tcagggtttt catcggctcg aaggcggtaa tgcggtcgag
     8761 ctggagaaac gggtagcggt gggggatgag tttttggatg tctttggctt cgatggggag
     8821 ttgtacgtcc atgtctgtcg tattccttga ataaagtcgg tttggattta ttgcgtgtct
     8881 ttgctgtctg aaagctgctg ttccagcgtt ttgaggcgtt tgttcatttc gcttaagcgg
     8941 tggatgtaaa cggcgttgcg cgcccattct ttatgggtgg acatcgggaa gatgccggcg
     9001 atgtgtttgc cgctttcggt aatgctgtgg gtaacggacg tgccgccgcc gatggtggtt
     9061 ttgtcggcga tttcgatgtg tccgaccgtg ccgactccgc cgccgatgat gcagtagctg
     9121 cctatggtta cactgccgga gatgcccgtt ttggcggcga tgacggtgtg cgaaccgatt
     9181 ttgcagttgt gtccgatttg gacttggttg tcgattttgg tgccgttgcc gacaatggtg
     9241 tcgctcatcg cgccccggtc gatgttggtg ttcgagccga tttctacgtc gtcgcccagc
     9301 gttaccgcgc cggtttgcgg gattttgaac cacgaatcgc cggcgaaggc gagtccgaaa
     9361 ccgtccgcgc cgatgaccgc gccgctgtgg atttcgacgt gtctgccgag tgtgcagccg
     9421 taataaacga cggcgttggg atgcaggacg acttcgtcgc ccagtttgca atcgtgttgg
     9481 acgacggcgt ttgccaagat gcggcagcct tcgccgagca cggtgttcgc gccgatgtag
     9541 gcgttcgcgc cgatttcaca gcttgcggga acggtcgcgc ccggttcgac gacggcggtc
     9601 ggatggatgc cgccgcgcgc tttgacgacg ggtgaaaaca ggcgcgcgac tttggcgaaa
     9661 tagagatagg gatcgtcggc gacaatcagg ttgcgccctt caaatccgtc tgctgctttg
     9721 gcggaaacga tgatcgcgcc cgcgctgctg tcgtggactt cggctttgta tttcggattg
     9781 gcgaggaagc tgatgtgttc cgcctgcgcg tctgcgagcg ggcgcacggc ggtaacggaa
     9841 atgtcctcgc cgcgccattc gccgccgagc cgcgcggtga tttgggacag ggtgcaggtg
     9901 gccggaatca tggttttcct gtttggtatg ccgtctgaaa gggttagcgg gcgttcattt
     9961 ctttaatgac gctgtcggta acgtcgtatt gggtgttgac gtaaatcacg tcctgcaaaa
    10021 tgacatcgta accttcctgt ttggcgattt tgacgatgac gcggttggcg ttttgctgga
    10081 gggaggcaaa ctcttcgttg cggcggaggt tgtagtcttc ttcaaactgc gcctgttttt
    10141 tgcggaacgc ttcgaccagc ccgcgccatt tttcttcggc ttgcgccttt tttgcgtcct
    10201 taagtttgcc gccggcgagc tgcctttcca aatccaagcc ttcgcgttgc agtttttgca
    10261 attcgtcctg acgggcggaa aattcgccgt ccagcgtttt ttggatgttg cgcgcctgct
    10321 tggattcgag gtagatgcgc tcggtgttga taaagccgat tttttggaag gtgtcggcgt
    10381 gcgtgcctgt ggtgcagcat aaaccgatca gggtggcggc aaacgcgcgg gtcaaacggg
    10441 tcatggtaaa actccttcgg atgttgccgc gaaatgccgt ctgaagggct tcagacggca
    10501 tttgcgggat tagaacgtcg tgccgagctg gaattggaag cgttggattt cgtcttccgg
    10561 ttttttcttc agcgggtagg cgtagctgaa tttcatcggg cccaaaggcg agagccaggt
    10621 aaccgcgccg ccggcggaat agcgcaattc gttggtaaag gtggatttat gcgcgttttc
    10681 cgagtaaacc gatttgttgt taccgttttc ggcggcggta taggttctgc cgtcccacac
    10741 gctgcctgcg tcggcaaaca ggctcaggcg gacggtgcgt gcgtctttcg caccgggcat
    10801 cgggaagagc agctcggcgg agacgttggc ttttttgttg ccgccgtagc tgattttttc
    10861 gccgtattcg tcatacactt tcgggccgag cgtgccgctt tcgtagccgc gcaccgaacc
    10921 caggccgccg ccgtagaagt tttcaaagaa ggggatttct ttggttctgc cgtagccgcc
    10981 cgcaatgccg acttcgccgc cgagcatcag cgtgaaggtt ttgcttaagg ggaagaacca
    11041 ggtttggttg tgggtggcgg agtagtattg cagtttgctg ccgggcaggg cgatttcggc
    11101 atttacgccg gtcaggtagc cgcgcgtcgg ccataaggcg ctgtcggtct tgttgcgccc
    11161 ccagccgaca gtgcctttgt acagcaggcc tttgaagctg ccgtctgcgc cgtcggtttt
    11221 gccgtattgt ttgataaagt cggcatagcg tttgggtgct ttgttgtagg tgttgacggt
    11281 caggtgttcc gccgccagcc cgaaattgac gcggtcgtat tcggtaacgg ggatacccat
    11341 ccttacgccg ccgccggcgg tggtggtttt atattgtttg acgctggtcg atgctttgcg
    11401 cgggtcgaag gcttttccgt aaatatcgta gcccaggctg accccgtctg ccgtgaagta
    11461 cgggtcggta aacgacagcg agccgttgag cgtggttttg cttcgcgagg cgcgcagggc
    11521 ggccgacttg cccgtaccga acaggttgtc ctgcgatacg ccggcggaca tgaccaagcc
    11581 ggtatcctga acccagcccg cgctcaagtc gagcgagccg gtggagcgtt cggtcaggct
    11641 catgttcaaa tcgactttgt cgggcgtgcc ggcaagcggg acggcatcaa actgtacgtt
    11701 gtcgaagtag cccaaaagct cgacgcgctc tttggaacgt tgcagcttgg aggtgtcgta
    11761 aggcgcggat tccatttggc gcaattcgcg gcggacgact tcgtcgcggg ttttgttgtt
    11821 gccggtgatg tggatttcgt tgacgtagat ttttctgccc ggttcgatgt gcaggacgaa
    11881 atcgacggtt ttggttccgg cgttcggcag cggctgtacg ctgatttcgc tgtatgcgta
    11941 gcctgccgag cccatgcggt tctgaatctc acccaaaacg gcggtcatct gctggcgttc
    12001 gtaccatttg ccgggcttca tggtcagcag tttttccagt tcggccttgg ggacttcgtt
    12061 ggtgtcgcct tcaatcgaca ctttgcccca gcggaaacgt ccgccttcgt ggacggtgat
    12121 tttgatggtc tgcctggttt tgtcttcgtt ggtttggatg tcggtatcga ggatacggaa
    12181 atcgaagtag ccgttgttct ggtagaagtc ggttactttt tccatgtctt gggcgaattt
    12241 ctggcggtcg aaccggtcgc ttcgtgtcag ccatgtccaa atgccgcctt cggtcagcga
    12301 catctgccgc atcagtttgc ggtcggaata gacttggttg ccttcaaatt cgatgtcggt
    12361 gattttggcg gatttgccct cgtcaatcgt gatgtcgatg tcgacgcggt tgcgggcgag
    12421 tttggttact ttgggcgtga tttggatatt gagtttgccg cgcccgaggt attcttcttt
    12481 caggccggcg actgcctggt tgagtgtcgc ctgattaaag tattgcgact gcgccagccc
    12541 gaacgattcg aggtttttct tgatggcgtc gttttgcagc attttggcgc cggtgatgtt
    12601 gagcgagccg atggtggggc gttcgataac ggtcagcaga agctgcccgt ccgcagtttc
    12661 gactcgtacg tcgtcaaaga aaccggtggc gtacaggctt ttgatgatgg cactgccgtg
    12721 tgtgtcgttg taggtgtcgc cgactttgac gggcaggtag ttgaatacgg tgctcggctc
    12781 ggtacgctgc aagccttcga cacggatgtc ttggatggtg aagtcggcaa atgccaaagg
    12841 cgatatgccc aacatcatca gtgcggaggc aatctgtttc agtttcatcg taagttcctt
    12901 gtggtgcgga atgcggtttc agactgcatt ccgaaacgta aaatctaacc gatcagccgg
    12961 gtaacgtcgt tgaagaaggc ggccgccatc atcagcatca tcagggcgag cccgaagcgc
    13021 aaaccgatgt tttggacacg ttcgcccaaa ggtttgccgc gtatccattc gacagtataa
    13081 aacacgaggt gcccgccgtc caaaacgggg acgggcagca ggttcagcac gccgaggctg
    13141 atgctgacca acgctaaaaa ttccaaataa ctttgcaagc cgagttcggc ggactgtccg
    13201 gcaatgtcgg caatggtcag cggcccggaa atatggctga cagaggcgtt gccgctgatt
    13261 agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc ccagcccatg
    13321 ccgaatgcgc ggacaacaga cggacggtag ctgcggcgga tttgcgcgtc ccacgccctg
    13381 tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcgggctg ttcgacagta
    13441 tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtaggtcag ggtgattttt
    13501 ttgcccgggc tttggcgggt caggtttgcc cattcctgcc atgaggcgat gggtttgccg
    13561 tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg gctgcctttt
    13621 tccacgccgc cggcaacggt tgtgatttta aagggcatca gtccgatgta gccttggttt
    13681 tttgcgattt taccggcttc cggcgtgcct gcggcatcga tggtgcggac ggtttgcgcg
    13741 cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag gacgatttcg
    13801 gtttgcgcgc tgctccagtc ttggacggaa acgccgttga cggattgtat tttgtcgccg
    13861 ctttggaagc cggtgcgggc ggcaatggtg tcgggttcga ctgtgccgac atagggccgc
    13921 agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag tgcgaggttg
    13981 gtcagcggac cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt gtcaaaagcg
    14041 tagggtaaat cggcttctga tacttcgcct tcgcgcgtat cgaccatttt gacgtagccg
    14101 cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt gaaaaacggt
    14161 ttgccgaagc cgacggaaaa acgcacaacc ttgacgccgc acaacctggc gacgatgtag
    14221 tgtccgaatt cgtgcaggct gaccaaaatc aggatggcga agataaaagc tagaagggtt
    14281 tgcaaatggt tttcctttga taacggtgtt cggacggtat cagcgcagtg tgccgataaa
    14341 tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaac agtccttcta tatcgcccat
    14401 gccgtttgaa aagtcttgtg caagacagtg ggcgacggtt ttggcaatgt cggtaaactt
    14461 aatctgtccg tccaaaaagg cggcgacggc ggtttcgttg gcggcgttca atacgcaggg
    14521 cgcggctccg cctgcgttta tggtttcata ggcgaacttc aggcagggga agcggccgaa
    14581 gtcgggcttt tggaaggtca gcgcggacaa tgcgccgaaa tcgagtttgc cgacacccga
    14641 atcgatgcgc tcgggcaagc ccaaacaata ggcgatgggc gttcgcatat cgggattgcc
    14701 cagttgcgcc agcacggagc cgtcgcggta gcgcaccata ctgtgtatca cggattgggg
    14761 atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg cttcaatcag
    14821 ttccaagccc ttgtttgcca tagtggctga atcgacggag attttgcgcc ccatacgcca
    14881 attggggtgt ttgaccgcct gctcgggcgt aatgctgtcg aacgtgctta aatcggttgt
    14941 taaaaacggg ccgccggaag cggtcaggat aatcgaatcg atgccgtgtt cgttcagacg
    15001 gtctgtgtaa tcgcgcggca aaacttggaa aatggcgttg tgttcgctgt cgacgggcaa
    15061 cactgccgcg ccgtttgcgc gggcggtttc cataaacaac gcgccggaaa ccaccagcgt
    15121 ttctttgttt gccagataaa tggttttgcc tttttgcgcc gctgcgagcg cggaaggcag
    15181 ccccgccgcc ccgacgatgg cgcacatgac gccgctgact tcgtcggcag acgcaacgtc
    15241 aaccaatgcc tgcgcgccgt gtaaaacctg agtcgccgtg ccgtcgcgtt tcaacagggc
    15301 ttcaagccgg gcggcgtgtt cggcatcggc aacgacggca tattcggggc ggaacgtttg
    15361 acattgagcc gccagtttct cgacctgctt atgccctgcc agcgcgaata cgcggaattt
    15421 ttcgggatgg cgggagacaa cgtccagcgt gctttcgcct atgctgccgg tactgcctaa
    15481 tatggtcagg acttgtggtg tcataatgag gataacttta taccggatgc cgtctgaagc
    15541 gttttcagac ggcatagaat caatttaaaa ccgacattat cgccgcatag acgctgataa
    15601 cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccgggcagc aggttgctgc
    15661 tgtctttgat gcctgccgcg cgcttgagcc agctttccaa caggtcgccg catacgctga
    15721 caacggtcaa caccaaaccg attaacacgg tatcgaacca gcctgtatcg aatgccagcc
    15781 agccggcact tcgtacggca gtcatgtaca ctgccacgca aaccgcaccg ccgattgcgc
    15841 cttcccagct cttgccgggg ctgattgccg gtgcgatttt gtgtttgccg agcgccttgc
    15901 cgctgaaata cgcgcaaacg tcggcaaccc agaccaaacc catcacggcg agcagcggca
    15961 gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc atgagcaaaa
    16021 gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat ctcaaccaca
    16081 aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta ggcagcatcc
    16141 agccgcccgc ataggcaact acgccgaaaa ccaaggttgc ggcgaggtaa tggttggttt
    16201 cggttttgca caaaccggcc atacgggcat actcccacaa ggcggtcagg gcaatcagcc
    16261 cgcaaaatgc agcccacagc cattgcggcg cgtaaaacag catgcccagc atcagcggca
    16321 gcagccacat agcggttatt acccgttgtt tcagcatatt cagttccttt gctgtccgat
    16381 aggcagttgc tcggaagtgc gtccgaaccg ccgttcgcgt ttttggaacg aagcgacggc
    16441 ggcatctaag gcggtttcgt cgaaatcggg ccacaggatg tcggtgaaat acagttctgc
    16501 atacgccatc tgccagagca ggaaattgct gatgcgcgtt tctccgcccg tgcggatgaa
    16561 caaatccggt tccggcgcgt cgcccagcat caagtgtttg gccagcgtgt cttccgtaat
    16621 ctcggatacg ccttcggcaa tcagtttgtt tgccgcctgc aaaatatccc agcggccgcc
    16681 gtaatccgca gcaatgctca gggtcagacc ggtattattt gccgtcaagg cttccgcctc
    16741 ttcgatacct tgcagaatct gccgattaaa gcgttcgcgg ctgcccaata ttttcaggcg
    16801 catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa acagccccat
    16861 caggaacgaa acttcgtctt cggggcgacg ccagttttcg gttgaaaagg caaacacggt
    16921 cagatattgc acgcccagtt tggcgcaatg cttcaccata ttttccaacg cgtccaagcc
    16981 gcgtttgtgt cccattatac gcgggagaaa acgttttttc gcccaacggc cgttgccgtc
    17041 cataattacg gcgatgtgcc tcgggatggc ggtgtgttcc aaaatggtct gcgtgctgct
    17101 cttcatatct gcctttcgcg gttcggcgtt caaatgccgt ctgaacgccg cgccgtgaga
    17161 tttaaaccgc catcaggtct tcttctttgg cagccaaaac cttatcggct tcggcaatgt
    17221 acttgtcggt cagtttttga accgcctctt cgccgcgacg tgcctcgtct tcggaaattt
    17281 ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg ttgcggatag
    17341 agacgcgtcc ttcttccgcc tcgccgcgca cgactttaat caggtctttg cggcgttcct
    17401 cggtcagcat gggcatcggc acgcggatca aatcgccgac agctgccggg ttcagtccca
    17461 agtttgaatc gcggatggct ttctcgactt tggccgccat attgccctca aacggtttca
    17521 cgccgatggt gcgcgcgtcc aaaagggtta cgttggcaac ctggctgacg gggaccatgc
    17581 tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg ccggtacgca
    17641 ctttcgccag attttctttc agtacttcga ccgaacgctg catcttgcct tcggctgttt
    17701 tttgaatatc gttgatcata ttgttctttc ggtaagataa ggtaggcggg cagccgttcg
    17761 aacgcgctcc aagccgttca gacggcataa agaccgttaa ctgcgaatag taccgttatt
    17821 cgggcatgac gacaaggcag atgcctgtag gaagggtgtt tcacctttct agggtttttt
    17881 taattgccgc tgcaatttcg taggcaaggt tgacggggac ggcgttgcca atcattttgt
    17941 atgcgtcgtt gacattttga tagatgaatt tgaagttgtc gggaaagcct tggattcttg
    18001 caacttcgcg taccgtcatc ctccgatata gtgtttcttt gccggcagca aaacggtagt
    18061 cgtttgcccc gtgcttttcc atctttgggg cttgcgggtg cagttggcac tgcctgcctg
    18121 aagcttggac ggtaaaaccc tgttcatccc acgctttaac tcggttccgg ctcataaaaa
    18181 taggggaaaa actgccggta aaatattcgt tgttgttgac tgcgtcgggg ttggtcttgt
    18241 tttgcggggc ggaaggtacg gctgtgtcct gcaaatccca aataacgtct ttcaatgtaa
    18301 tcttgtcttt gtcttcgacc gtcgaacctt ttggaaaaga aaattttatt tccaagtctt
    18361 tacggaaacc gatgtagaag acccttttgc gttcctgtgc tacaccgtag tctttggcgt
    18421 tggccatagt caaggttacg tcgtatccgc atccgtcaaa cattttcagc aggttttgta
    18481 cggctccgtt gtggcggttt gccagcattc cgctgacgtt ttccgctaaa aagaattttg
    18541 gctgtttgct tttcaaaata cggatgtagt cgaaaaacaa ctgtccgcgc gcatcgtcga
    18601 tgccgcgcaa agctcccgct tcagaccaag actggcaggg tgggccgccg ataatcccgt
    18661 cgatttcttc agggaaatct tcttctttaa tcttgcgtat atcgccttct atcaaatggg
    18721 tctttggatg gtttgccttg aaggttgccc aaatggtttt atcgtattcg ttggcggcgg
    18781 ggatttcaaa tcccgctttt tcgaaaccca aatccaaacc gccgcaaccg ctaaacaaac
    18841 taatgatttt cataggatta taagtggtag gtgatgagtt ttgcatttct taatcgcgcc
    18901 gggttgttgg gatttttaat tttaatgtcg gaaatggcca gtttggaatc ctgtatggca
    18961 agcagcttgt ctgtattgtt aaaactgttc catttgtctt cgttgataat ggccatgaaa
    19021 ttgaaacttt tttccatatt ccgttggtaa atatagttga agacaaacca tggattttcg
    19081 attccccaca taccccgtac tctcagataa gttatgttga gcggatcaat gcggttgacc
    19141 ctgcccagct ctttggtttc tgcaaactgt atgccgccga tgttgccgat tccttcctta
    19201 atttgatttt ttatcttcaa atagcattcg gcatcggcgc agtagtcgat tccgtaaacc
    19261 attgccaagt gtttgaggtt tttctttttg tcaacgacgc cgacaattat agataatgtc
    19321 tttctcttcc catttttccg catctttgca ggcttttgta agcatgctgt catcaacaga
    19381 gagctttgat tttgggtggc tgctattcag cgccagtgcg gaatctttac tttcaatttt
    19441 cttaacttca atggcatccc cgtttcgcaa catggcatca ggagggttgg agttgttgcc
    19501 caaatatgag aatacttttg cgtgtcgggc tatgcgttgg gtttcattta ggttgaaact
    19561 gccggaaaac aagtctttta catattcttc caaagcatca cctgcctgat tggcacg
//
LOCUS       DAXDSD010000034        19350 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXDSD010000034 DAXDSD010000000
VERSION     DAXDSD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19350
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.034"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            133..978
                     /locus_tag="ACFB2Q_000935"
     CDS             133..978
                     /locus_tag="ACFB2Q_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC0917984.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTASGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGYQITPKTTADDRTGLRQAVAALVGRKGIDYSSAYSMIHQRFNVESIEDLPAGK
                     LPEAVAYVHALTLHTGLTGEVLDAPPKAEPKLPIDGNSLADIAAMVYYGTWMIESGKD
                     ISAPLKQLGCRQAVTMWTVWHETRPILKRSAATLEVLRGYADKDASDRIAACLEGIYG
                     KATVR"
     gene            complement(1015..1164)
                     /locus_tag="ACFB2Q_000936"
     CDS             complement(1015..1164)
                     /locus_tag="ACFB2Q_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917985.1"
                     /translation="MSAEEFCRKQIAYWLNESRKASDNADLKAFEFAGREPADYREML
                     KRYAA"
     gene            complement(1167..1841)
                     /locus_tag="ACFB2Q_000937"
     CDS             complement(1167..1841)
                     /locus_tag="ACFB2Q_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917986.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDAPHL
                     NRHFWI"
     gene            complement(1838..2323)
                     /locus_tag="ACFB2Q_000938"
     CDS             complement(1838..2323)
                     /locus_tag="ACFB2Q_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917987.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIA
                     Q"
     gene            complement(2329..2802)
                     /locus_tag="ACFB2Q_000939"
     CDS             complement(2329..2802)
                     /locus_tag="ACFB2Q_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917988.1"
                     /translation="MARIHVRNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADAYG
                     PPKAGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAHVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(2857..4152)
                     /locus_tag="ACFB2Q_000940"
     CDS             complement(2857..4152)
                     /locus_tag="ACFB2Q_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFC0917989.1"
                     /translation="MAQKTNTAYGDPQAMMKQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGAR
                     GFHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVGDNGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELDHGNRVETLVGT
                     IRGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(4173..4910)
                     /locus_tag="ACFB2Q_000941"
     CDS             complement(4173..4910)
                     /locus_tag="ACFB2Q_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917990.1"
                     /translation="MGGIAGGRQYGRGGRRGYRRGGRRADKVIRAKDGKHTIPYEKLV
                     EAREAEKSAKAEAQALRERIEELEKGAEKPGVETADGSDNSLFGDFSDEDVKKGVEKL
                     IQEKLAGYEADMKRQEAAKAHYREIYTAHPDADSIVESRELEEWLAGQNPLVRKAFND
                     ALKDGTAAEVIGAFDMFKAAKSAAEPEKPAEKPPAGKNTPNTLSDIPAGRDHTASDGP
                     PDYLSGNALAEKLASMTEEQVEKFLNS"
     gene            complement(4892..9700)
                     /locus_tag="ACFB2Q_000942"
                     /pseudo
     CDS             complement(4892..9700)
                     /locus_tag="ACFB2Q_000942"
                     /inference="COORDINATES: protein motif:HMM:NF032893.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
     gene            complement(9642..10994)
                     /locus_tag="ACFB2Q_000943"
     CDS             complement(9642..10994)
                     /locus_tag="ACFB2Q_000943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917991.1"
                     /translation="MRRHRRLRTAAQAAADGGAEQGGAGFDTAHHDQSHPALRQFADR
                     TKQEEAGRFFSGPADGNTPHAEELARGTEKQPDVSGIPSEDGAEFLDTGVMPDGLARQ
                     YADMAAKYRAKPSEAVGIDPDDGAVSAAAALAADSGAAVPSAVSDDMEARSVADDAPS
                     GRSADADRGGVPSAYGNVRPGGAPRGAASVAPGGSAAAASGGIARVSPLPAGQYFDGL
                     DTRGRKALAKEAGLDIKGVADFGQIAAPVRRKIEQAYHARIEADYQAASEAKQGYLPP
                     PVRMADAVPVPKKGFSVPADALDKESRRRFDALPEGVRRHAQTVADYTADGIMRREAG
                     MADMRGHYPEGLAESAGAVRAYRAEHPESADVLDRLNRAVYGYRRNNGWSVPLLSREG
                     ERLQGVRTALPDDGASEAVVGGGRGLTRALPTEDKGLAQDVRQDVRQGFDPRRQGFDP
                     "
     gene            complement(10915..12129)
                     /locus_tag="ACFB2Q_000944"
     CDS             complement(10915..12129)
                     /locus_tag="ACFB2Q_000944"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917992.1"
                     /translation="MSDLVRYDPLEHGRLAGGLKEYRGFTQKDARAAADDTALTRGFK
                     NSMRSARMGWNALTGDKEELGRLKAEDMDYRKIQEGRKSQARRELGEAWEKGGGVGGG
                     LSNVWGELKKDWREKGLDGALEDVGEMGGAVLEQAPNALVPIATTTAGGILGALAGGN
                     AAVGAYAGATLGNTLMEYGGQLDRAAEAAGVDPADKDAVMAFIGRGAPGALKNAAVKG
                     AVVGAADMAAMKLGGSILNMGKKAAGKAALEKMGVAAADKAAVAAAKGTPEFAALAKE
                     SAKGGLGGAARHAAAYATESAGEFAGEYLGTGLANGEWDEKGAALEAFSSLGHSAVGF
                     AGTKAYAAVTDPLRPPAGRKAGAQGGIGGNRKAGRPAPEGAQALRAAAQAAADGGTGG
                     CGRRRGTGRRGF"
     gene            complement(12126..13322)
                     /locus_tag="ACFB2Q_000945"
     CDS             complement(12126..13322)
                     /locus_tag="ACFB2Q_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917993.1"
                     /translation="MGLFEPSAGDFWEMKEKEKKEKARKGAEERERAAAQAHRADAVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKALSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRDGKPVRDFDAQRPLVSAGPDALSPEE
                     RELYRRATTPYAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REAVLQEGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LEDRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFERQGEKGFDPNVFKTISYEVA
                     DPDTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAQLGFKPNGQKTAGGKIIYENEKG
                     EKRVEQ"
     gene            complement(13559..15826)
                     /locus_tag="ACFB2Q_000946"
     CDS             complement(13559..15826)
                     /locus_tag="ACFB2Q_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917994.1"
                     /translation="MEALLMGTDVPETGVLPDKNGEPLTIGEYRLFVGEMMNQPAWRA
                     VADKEMDYADGRQLDNELLQKQRELGLPPAVENLITPTLLSVQGYEATIRTDWRVTAD
                     GETGGRDVADALNFKLNRAERQSRADKACSDAFRGQIACGIGWVEVTRNPNPFEFPYE
                     CGVIHRNAIHWDMKSYKYDLSDARWLIRRRWLLPERLAQFFPEYAGHFKAMGRGGSDW
                     RISGEMLDGGGNTGLADAWGISGRNTVSEEFWFNETTRELAVAEVWYRRWVTADCLRD
                     KKTGRTVEFDGANPNHREMAANGAVLFAASVPRMRRAFVVGDLVVRDEPTPYPHQKFP
                     YVPFFGFREDNTGIPYGYVRNMKYAQDNLNSTNSKLRWGLSAIRTVRTKGIVDMSDEQ
                     FRRNIARVDADIVLNKIEAAQPGARFDVSRDFELSAQHWQMLQDSRATIRQISGITPS
                     FMGNRGNATSGRQESIQVEQSNQSLGLVMDNFRQSRSLVGELLLAMIIEDLGSDEQTV
                     VIEGDAVTQGRTVVINRPETDPVTGKAYLSNDLQNIRLKVALEDVPSTNSYRSQQLGA
                     MSEAVKSLPPEYQAAVLPFMVSLMDIPFKDKVIEKIKEVRVQETPEQIEARIAQAVQD
                     ALAKSGNDIKRRELALKEQRTASEIKEIEARAVQIGVQAAYAAMQAGGQIAAMPQIAP
                     VADAVMQGAGYVRPARGDDPGFPVPAMPPETQIPPEGIPEAYGADTGPMTAVPPKSAN
                     HAQTGMETPTVSDNL"
     gene            complement(15811..17085)
                     /locus_tag="ACFB2Q_000947"
     CDS             complement(15811..17085)
                     /locus_tag="ACFB2Q_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFC0917995.1"
                     /translation="MTGKTVDLKLPAKLDGLFKPCRYKVMYGGRGGGKSHGAASALLA
                     LGAQRPLRILCVREIQKSMRDTVHRLLKDKVAQLGLGHFYEITDFEIRGANGTLFVFS
                     GLQSHTVDSIKSFEGIDIVWVEEGHGVSKKSWDVLTPTIRKEGSEIWITLNPDMETDE
                     TYRRFIAMPSEDTWLCEINWRDNPWFPEALNRERLKAQRSMNKEDYGNIWEGRPRMVS
                     EGAVYRHEIQDAFHSGRVTLVPYDSSLPVHTVWDLGWNDAMTIGLVQRDLTSVRIIGY
                     IEDTHRTLDWYVAELEKLPYRWGTDFLPHDGRTRNFQTGKSTMEILTGLGRKSVFVQN
                     ATGIEEGIRAARMLFPKVYFDKDKTARLLECLKRYGRQIHAKTGVAMGPLHDEYSHGA
                     DMFRYLAQAVDLMDTGSNTGYTETPVSDWRLY"
     gene            complement(17066..17605)
                     /locus_tag="ACFB2Q_000948"
     CDS             complement(17066..17605)
                     /locus_tag="ACFB2Q_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917996.1"
                     /translation="MPRLSDEDWRKVELDYRRGVLSIAEIGRKYNVSAQHVGRVAKER
                     VWTRDLNDEVQAKARAMVLSADKNGNAPDYADFNLKQVTDAEAKRVAAVQRRHRNLAE
                     NLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIERKSYGMDTAESKISESA
                     KTAGIRIEFVGPEDDGKDG"
     gene            complement(17605..18027)
                     /locus_tag="ACFB2Q_000949"
     CDS             complement(17605..18027)
                     /locus_tag="ACFB2Q_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0917997.1"
                     /translation="MSGLSDIDKVYQGVVDLHNAEQVVSREALAETTGLKMSEITKFT
                     KLLVEHGKIYRVTRGIFKPAIGFGETRPVSVSVLDSGMGVLEIGDTVLHLNPQEMRSL
                     GALMSGFGQQFSSIQMGREFSVLRNYLECSAKNGRLDL"
     gene            complement(18092..18610)
                     /locus_tag="ACFB2Q_000950"
     CDS             complement(18092..18610)
                     /locus_tag="ACFB2Q_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC0917998.1"
                     /translation="MGLTQEVLKELLRYDDNTGKLYWAERPRKYFNSGLHYKSWNTGF
                     SGKEVFLYKGRLGYLKLKIFKKQYNAHRLIWLFVYGKHASSIDHINRDKTDNRISNLR
                     DVTHAENMKNRGKFKNNTSGHTGVYFHKPSKKWQARIMVNRKNKILGLFEHIEDAVKA
                     REAASKDFGFVV"
     gene            complement(18601..18984)
                     /locus_tag="ACFB2Q_000951"
     CDS             complement(18601..18984)
                     /locus_tag="ACFB2Q_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFC0917999.1"
                     /translation="MNGAEFTLTPQNKKQVMRSIWDSPDGWFENGNLEITIRPRKSKR
                     SVEQNRRLWFLYREISEKVFIDGRRFSQDVWHEFLKRKFIGCIEMPNGQLMGISTTKL
                     SVREMSEYQEKIISWASMEHGVLWD"
     gene            complement(18981..19286)
                     /locus_tag="ACFB2Q_000952"
     CDS             complement(18981..19286)
                     /locus_tag="ACFB2Q_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HFC0918000.1"
                     /translation="MSSAIRKAAKGGQCTPNIAGVCNDNPETVVLCRFPGETHGAGLK
                     SGGLGAGFGCGCRRGAIDGRGAGLSREDKEFYMRRSQLRTIRRLEALGVVGVKGRLK"
BASE COUNT         3943 a         6835 c         4510 g         4062 t
ORIGIN      
        1 attcttacaa cggcacagcc gttaatggaa aaccgaacca agcgggaact tggttcggtt
       61 aagtgaaaag cagttctagg aaaactcttt tcgcgtcttt taaataacct ctaaaagaaa
      121 gactattcta tcatgaatgc tgtccaagtt ttaaactttc aacaaaactc cgtccgtact
      181 gttgccgata acaaaggcga gttgtggttt ttggcaaacg atgtttgcga gattttaggc
      241 tacaccaacc ctcgccgaac ggttgacctg cactgcaaaa gcaggggcgt aacaaaacgg
      301 tacaccccca ccgccagcgg cgaacaggag atgacctata ttaacgagcc gaatctctac
      361 cgcttgatta tcaaatcccg caagcctgcc gcagaagcgt ttgaagaatg ggtaatggaa
      421 accgtcctgc ccgccatccg caaaacaggc ggctaccaaa ttactcccaa aaccaccgcc
      481 gacgaccgta ccggattgcg ccaagctgtt gccgcgcttg tcggacgcaa aggtatagac
      541 tactcctccg cgtacagtat gatacaccaa cgcttcaacg tcgaatccat cgaagacctc
      601 cctgccggga agctgcccga agccgtcgcc tacgtccacg cattgacact gcacacgggc
      661 ttgacaggcg aagtgttgga cgcaccgccc aaagccgagc cgaaactgcc catcgacggc
      721 aactctttag ccgacattgc cgctatggtt tattacggca catggatgat tgaatcgggc
      781 aaagacatct ccgcgccgct gaagcagctc ggctgcaggc aggcggttac gatgtggacg
      841 gtttggcacg aaacccgtcc aatcctgaaa agatccgccg cgaccctcga agtgttgcgg
      901 ggatatgcgg acaaagacgc ctccgaccgc atagccgcat gtcttgaagg catttacggc
      961 aaggctacgg taaggtaaac ggaaaatgcc gcccgaatct tcggacggca tttttcacgc
     1021 ggcatagcgt ttcaacattt cccgataatc cgccggttcc cgtccggcga actcaaaggc
     1081 cttcaaatcg gcgttatctg atgccttgcg gctctcgttc agccaatagg cgatctgttt
     1141 cctgcaaaac tcttcagcac tcatcgtcaa atccaaaaat gcctgttcag gtgcggcgca
     1201 tcctcacgcg tgatacggcg caaatcactg tcggccggca ggccgaaata cagctcgaaa
     1261 cgcctccttg ccgattccga acgctgcggg tcgaaagtct ccgcatccgg gatactgaaa
     1321 gcctccgcca aagcccaata aaccaaattc cgatgatgta tggaattgat ttccggctcg
     1381 tccccgtccc ccgccatatc ccgcggcagg caataccccc cggcaaacaa cctgccgtca
     1441 cggtccggcg caggcgcaag cgtcagcttc ccgtcgccct gcaccgcata aaccggcagc
     1501 cccgtttgcg ccccgtcgcg ccaacccggg gcgcaaacat ccgccgcctc cggcgaaacc
     1561 aacttcaggc acaccggctt gccgtcggac acaaaacgga tactgtcgat ttcatacgca
     1621 taacccgccg caaacccccc tgccgcataa gcataaagcg gccgcccttt ttccacatcc
     1681 gttacaaacg catcgtccga atgcagcagc cggccgcgca ggcacgcctc gtgaaccgca
     1741 tcgttcaacc atcccgacac ctcttcgtcg ctccaaaaat acggctcggc cttatcattg
     1801 gcaagcacgc ggaaacgcgc aatcaaagcc ttcaaattca ttgcgcgata ccgaactgac
     1861 gaaccatccg aaccacgtcc ccgcgcaaat tctccgccga tttgcgctta tccaaaccct
     1921 ggccgtaatt cgccttcgca tacagttcaa gctcgtcctt gcccatccgg ttgatttgca
     1981 ggatcacatc ctgcaccgcg tcaaacgccg ccgtttcccg ttcgagttcc gaagcgcccg
     2041 ccttttccaa agcctccgta tcatccgcct tttccacgcg tttgcccggg acggcggcaa
     2101 acacatcgcc gtggcgcaac aactttgccg cagccccggc cgacacctcg cgcacctggc
     2161 cgtcttcgaa aaccaagccc gtatgataga tacggtcgcg ccacacggca cgcccgccga
     2221 tatacttgat aaaaacctta tccatccaac cccccgccgc cgtccggacg gcattgtcaa
     2281 atacagcaac gccgcaaagg caaatccccg ggcggcgttt cattccgctt acaactggcc
     2341 gaacttctcg ccgtaaatca aaatatcgat atcggcggcc ttcttatttt ccgccccttt
     2401 aagcgtaacc gtcagcaaag cctgcttggg cagcgtaacc agtttggcag actggcagcg
     2461 gatacgcccg gcagacgcaa agtccgcgcc cgaagcaaaa taagccgcat cctgaggcac
     2521 gtgcgcatca tccgcaccgt ccgcatacgc aaagccgaca tcggcagtga taccggaaga
     2581 catcgccgtc ttcacaataa tctgcgcgtc atccaaacgg aaaccctgtt ccaagacacc
     2641 cagcgccaac acatcgcccg ccttgggcgg cccgtaggca tccgccccca agattgcacc
     2701 gtcctgcttc gcaacgatac ggtaatgctc cgccgccaga ttgccgtaag gcacgccgcc
     2761 gaaacggttg ccgccattgt ttctcacatg aatccgagcc ataaaccccc ccaactcgac
     2821 aaataggaaa agccgccctt tcaagcggca ttcgaatcag cggataccgc cgtgcaaagg
     2881 aaccaccgta tccacaaccg tgacaccgta gtcggtaatt tccttcgcac ccccgccgac
     2941 atccacggca aagcgcgttt tcgccacacc gcgtatcgta ccgacgagcg tttccacacg
     3001 gttgccgtgg tcaagctcct tctcagacca aaagaaaggc ataccgccgt gtttgccggt
     3061 attcgcaaac gcctccaaca ccgcctggcc gcccaaaata accgaacggt cgaccgcaaa
     3121 cttgtccgca aacgaagccg ggattttcaa gcccgattcc gcctcgctga acttatcggc
     3181 gcaatacttc atctcatcgc ccgcatagaa acggatgggg cgcggcattt tcaccaggat
     3241 aaagccgttc cacaaacccg catcgcccaa aaacagcgga ttttgatttg cctgggaggc
     3301 gcgcgcgatt gccgaagcct gaagctggcg gaatttcgga tcggcggcaa aacggttgta
     3361 ctgcgccggg gaaagcagcc acacgcgcaa aggcgaatca cccgccgcct tgtcgccctc
     3421 aaacttcaca atcggcggcg gcagcggaat ctggtcgagc acggtacgca tactgtccac
     3481 cgtatccatc gtaaacaaat cggtagaggc aatcttcaac tcgccgccgt tgtccccaac
     3541 gcccgttacc gcatcgcccg aagccgtaaa atggcggttt ttggacgggg ctttgaccgg
     3601 attgaccgca taatcattga atttcgggtc gcccgccaaa ggcacgcccc attcgatatt
     3661 gtcatgaaaa ccgcgcgcgc ccgccatatg caccaacagc gtctggtcgg cataacggtc
     3721 catcaggctt tgcgccgccg gacgaatcag cgcgcgataa tcggcagggc tgcgctgatt
     3781 cgtcatcgtg ccgccgccgt caacggggaa acgcgcctga ttcacacgca aacccgcctc
     3841 cgacaggctc atccccacgc ccctgccttc cgccgtgttg tcgcccataa tcggcagggc
     3901 ggaaacaggg ttgaccaaat tgaaacggat ttcgtcaccc atgccgcgcg tcaaatcctg
     3961 acagcgcacg accggcatat gctgggtcgt ctgtttgcgc aaagtcgcct ccgcacccgc
     4021 ggtaccggca ggcatcttgc ccgccaaacg gttcagcgta ctgttgcgct gcatatgcat
     4081 cgcaaacagc cccgccgcct gcttcatcat cgcctgcggg tcgccgtagg ccgtattcgt
     4141 cttttgtgcc ataaagagcc tccctcaaaa actcaagaat ttaaaaactt ctcaacctgc
     4201 tcttccgtca tggaagccag tttttccgcc agcgcgttgc cgctcaaata atccggcggg
     4261 ccgtccgaag ccgtatggtc gcgccccgcc ggaatatccg acagcgtatt gggcgtattc
     4321 ttcccggcag gcggcttttc ggcgggcttt tccggttcgg cggcggattt tgccgcctta
     4381 aacatatcga acgccccgat gacctcggcg gcagtcccgt ccttaagcgc gtcattaaac
     4441 gccttgcgga caagcgggtt ctgcccggcc agccactcct ccagctcgcg gctttccaca
     4501 atcgaatccg catcggggtg cgccgtatag atttcgcggt aatgcgcctt tgccgcctcc
     4561 tgccgcttca tgtccgcctc ataacccgcc agcttctcct gaatcagctt ttccacgccc
     4621 tttttcacat cctcgtcgga aaaatccccg aacaacgaat tatcggaacc gtcagccgtt
     4681 tccactccgg gtttttccgc ccccttctca agctcctcga tgcgctccct caaggcttgg
     4741 gcttcggctt tcgccgactt ttccgcctcg cgcgcctcta ccagcttttc ataagggata
     4801 gtgtgtttgc cgtccttggc acggatgacc ttgtccgcac gccgtccgcc gcgccgatac
     4861 ccgcgccgac caccgcgccc gtactgccga ccaccagcga ttccgcccac ttcttaccct
     4921 ccaaaccgtc ttggatatcg gggaaaacca actggtccaa acccaaacgc cccgacacgt
     4981 ccagcggcgt caggcggctg aaccccttgg ccaacacctc gcccgcctta tccccgaaag
     5041 tgtccgccaa catattgcgc aacgccgctt ccgcatccca cgggtcatcg tcgtcactgc
     5101 ccaacatcga aaccaccgca agcagcgtcg acacaaacgg cagccccaag atgcccgaag
     5161 ccatcgcatg cgagaccaac agccccgcca gcgtcttacg cgcctgctta tcgcccttaa
     5221 acgcaaggta cgcattgcgc cccagcgcat acaggatatt ctgcgaatac tgcttgaaca
     5281 gaaagaccac cttcgccgca ttgcccatca taaagcgcgg acggttttgc gccgcatagt
     5341 caaaatgccc gtcatacgtc gcctttttcg cctgttcgaa agccgcctcg ctgtccgccc
     5401 ccgcccgttt cgccaaacgg taggccgcga caaacgtaac ctggcggtta aacttctccg
     5461 catgatggaa cagccaggcc gccttatcca tcaccttctg ccaagccgac cccgccaacc
     5521 ccgggtcgcc gttggccacc cccgccaaat catgcgcctg cgacaaatcg atcacaccgt
     5581 aatccgccgc ctttcggaac gccgccttct catccccgtt caaactgtcc tcaatcgtat
     5641 tgaacttctc cccgaacctc agcccgatct gttttgaagc ccgcagcaat tcccgcgccg
     5701 caccggcata accccacttc gccgccatca ccggataagc caccattgcc gtctgcgaca
     5761 ggttcacaac cgcagaagcg ggcgacatcc ccatcatcca caaaaagccg aagcccgtca
     5821 aagcctgcgc cagcttggaa ggattcggat tcatcaccgc ctcgtggcgt ttcctcatct
     5881 catccgccac acgctgcaac tgacgctggt cgaaaccctc ctcatatttg cgcccgtcca
     5941 caaaatcctg catcacatcc aactgttccg ccatacggtc cgcatagcgc aacttcgcca
     6001 gataattcgc accgctgccc atattctgcg catatgcgcg cctcgcatca tcgctgaagc
     6061 ccggcacgcc cttgcggtgg atgccgtgtt tcgcccaaga cgtatcgggc agcgagttca
     6121 aataaagctg cgtcagcgta tcgttcaatc gggcccgttg cgccggatcc aaatccatcc
     6181 tgccgacagc ctcgcccagc tccctcataa aaccgccgct taccgcatcg cggcttcgga
     6241 tataatcccg ggacttcatc acgggcgaaa ccttaaaccc cgccccgaaa tcagccttca
     6301 gcgcatcgcg cagcttctcc gcctcgctca aagtttccgc gcgggacaca ttcgcactat
     6361 tcccgtccgc atccttgacc accaccagat aatcgccgaa acgcgccaag gggaaataca
     6421 caccgcccag ctcgttgtca aaacgctcct tcaggcggcg caccgtttcc gcatcctgcc
     6481 ccgcacgcgc caaccgttcg gcaagcgcat cgcgcacctg cccgaaatgc gccctataat
     6541 catcccgcgc cttgcggtac accctctgcg ccgcatcagg cagcccggca aaacggcggt
     6601 tcagcaaagc aagcccctcc aaagcctttt gcgccccaac ctcatccttt tccaaacgct
     6661 tcacatccgc gcgcgcctgc tccgacatcc gtaccgcatc cgttttagcc cggctttccg
     6721 ccgccacatc cgccccggcg cgcctcaacg cccgcttcgc ctccgaatcc gcataaaaca
     6781 gacggcgcag gcgcatatcc gcctcatccg ccaaaatttc ccgaccgtgt ttttcctgcg
     6841 ccttctccag cgcataagcc gccttatccg ccgcccgttg cgctttccgt accgcggcat
     6901 ccgcacgcgc ggtacgcgca ccggcggaag caacggccgc ctccgccttc gcgattttac
     6961 cgtccgcaat atccaaagcc gtccggatgc cgtccaaacg cttctgagca tcctttcgca
     7021 tcagcgggtc ggcatccacc ttcgccagcg tcgcatcgtg catcagatcc gcaagcgctt
     7081 tcgcatcctc ctccttcaac ctgccccatt ccctgacaac cttgtccgcc tccgtaaccg
     7141 ccttgttcgc atccgcgccg aagagttccg acaaacgccc gtattccttc aggccgtcca
     7201 aaacgccgcc gtaaatgtcc tcgatctgcc gcctgcccaa aaactggagc tgaatcccct
     7261 tggaagcatc ccagcggtct ttcagcgcgt ttgccaaatc ggatttttta cccgttatat
     7321 gaccgattgc ggccccgatg ttcgccgcac gggaaaaacg caccgcctcc gtgtcaaaat
     7381 cggcaatgcc gcccttgccc gcatctttgt cttgcaaaat ctgcaacagg cgtttatcat
     7441 caatgcccga taaaggcgat ttgctttcag aggctcccgt agttgatacg cggtcaagta
     7501 atgaaagcaa gttgcctttt tctattttcg tcagcttatg gtcgtaatag cggttaccgt
     7561 tcctgctaac cgcgacggca gcccttaccg tgtaatccgc accgccgaca ttcagtcccg
     7621 aaacataata ttcataaccc tgaatatcgc cgttcttagc caaatcctcg ttcggcagcg
     7681 tatcgatata caccgcattc tcaataatct gcggaattgc cgcgatactc tgcaaatgtt
     7741 ccgcgtcctt atagtcgtga cgcaatattt cagtgatgcc cgaacgtccc aaactgattt
     7801 cacgccccgt gtccttattc acataaggcc cgcgcaaaga tttgccgtat tccagcgcat
     7861 tgcgcttata ttcgcgcaaa ttgccgcctt ccggaacctc cctgcccgag atacggatgg
     7921 tttccgcacg gcgcagcttc tcaaggcttt ccgacttgga acgttccgcc gcaccgtctt
     7981 cggcccgcgc gaacatcact gcacgatgat tgccgcccca aggcgcatcc cgcgcccctt
     8041 ccacattccc gtgcaaatcc gccagcatcg caaacacatc cgcgtccgac atcgcaccgc
     8101 cggccttgcc cgccaggcgt tgcagcacac ggcgcaacag ggcgccgata cgcgccaaca
     8161 cgcccgcaat gccgcgtttc aaaccgttgc cgaccttcac gccgtaacgg ttttcaatgc
     8221 ccgcccaacc gccggtacgc tgcgccgcat aaagctccgc gaccgcctct tccaccgcgg
     8281 cggggcgcac cgaggccgcc gcatcgcccg tgccttcgcg cccttcctgc accgcgtccg
     8341 caatgcgccg aatcaggccg ttgccgtccg cacgttccaa ttcttcacgg tacttggcga
     8401 aaccatccgc cgcaaagccc ctgtgcccca gctcgtgcca cgccgcccat accgcacgtt
     8461 cgggcgtaag gttttccgcc accagcgtga ttttgcccgt cctgccgtca aaccagccct
     8521 ccacacgctg cgacaacagc atctgcgcct tgtccggcgc cgtttcggac acggcggcga
     8581 catccacccg ttccgccagt ccgcctatgc tttcggcgag gcgttttttg atgctttcgg
     8641 cgtgctcgcg caatgcctta aaaccttgcg gcgcctgacg ctctttcacc gtataaacag
     8701 gccctccctg caacacacgc gccttgcgcc gtgcctcttc cggattgtcc gaagccgttt
     8761 tcgcaaacgt ttcaggcttg gctttgcccg tcttttcaga cggcttttcg gttgccgcgc
     8821 cttgtgcggc ggccgacttg ccctttccgc catcgaagcg ggattttccc gcttcgatat
     8881 ttcgcacctt atccgaagac ggcttttcgg ttgccgcatt ttttgcagca gccgccttgc
     8941 ccgcaacagg cgtatctttt gcccgggcag gctgcgccct gcccgccggt ttgccgtcgg
     9001 cggcggcatc gcgcggccgc gccatcacct tggccggcgc ccttgcttcc tgcgtcggac
     9061 gataatcccg acccggctcg gacggcacgg cttccgacaa accgcccgcc cgcaaggttt
     9121 ccgcctcccg ccccttcaaa taacggcgac cgtcggagac aaggccgtca gagttggaag
     9181 gaccggccat atcggcaaac tcctccggca tgccgccttt gcccggcctg cccggcacgc
     9241 ccgcaagcgc atcaatatgc ctgcggtccg tatgctcgaa acggtattcg ccgcccccca
     9301 aaggcacacc cgccttttcc ggcctgcgcc tgtccgccgc caaacggcgc gtgaaatccg
     9361 ccgccgcatc cgccatccgt ttcttaggcg ataccctgcc cgccgattcc gcaacctgcg
     9421 gattcaatac cggcaacacc gtatcggggc gtttgcccgc aacaggcgca cgtttcccgc
     9481 cgtccgtccc ggcaaggttt tcagacgcgg acaggccggg cgcggcacct tccgcgcgcg
     9541 cccgaacctg taagttttgc cgacgggccg gcgcattgcc ggacgcaacg gcggaccctg
     9601 gcaaaccctg caaggcagcc gcatttgcat ccgcccccgc atcaggggtc aaacccctgc
     9661 cgccttgggt caaacccctg ccgcacatcc tgccgcacat cctgcgccaa acccttatct
     9721 tccgtgggta aagcccgggt caaacccctg ccgccgccga caacggcctc agacgcgccg
     9781 tcatccggca acgccgtccg aaccccctgc aaacgctccc cctcgcggct caacagcggc
     9841 acgctccaac cgttgttgcg gcggtaaccg taaaccgcac ggttaagcct gtccaacaca
     9901 tccgccgatt ccggatgttc cgcacggtaa gcgcgtacag ccccggccga ttccgccaaa
     9961 ccctcaggat aatgtccgcg catatccgcc atacccgcct cccggcgcat aatcccgtcc
    10021 gccgtatagt ccgccaccgt ctgcgcatga cggcgcaccc cttccggcag cgcgtcaaac
    10081 cgcctgcgcg attccttatc caacgcatcg gcagggacgg aaaacccttt tttaggaaca
    10141 ggcacggcat ccgccatacg cacgggcggc ggcaggtaac cctgtttggc ttcggaagcc
    10201 gcctgataat ccgcctcaat ccgcgcgtga tacgcctgct cgatttttcg gcgcacaggc
    10261 gcggcgattt gcccgaaatc cgcaacaccc ttaatatcaa ggcccgcctc cttcgccaaa
    10321 gccttgcgcc cgcgcgtatc caagccgtcg aaatattggc ccgcaggcag cggcgagacc
    10381 cgcgcaatcc cgcccgaagc agcggcggca gaaccgcccg gcgcaaccga agccgcaccg
    10441 cgcggcgcac cgccggggcg cacattgccg taagcggacg gaacaccgcc cctgtccgca
    10501 tccgccgacc gtccggacgg cgcatcatcc gcaaccgacc gggcttccat atcgtcagac
    10561 accgcagacg gcacagccgc gcccgaatcc gccgccaaag cagcggccgc agaaaccgca
    10621 ccgtcatccg gatcaatccc caccgcctcc gacggcttgg cccggtattt agccgccatg
    10681 tccgcatact gacgcgccaa accgtccggc atcacgcccg tatccaaaaa ttccgccccg
    10741 tcctccgacg ggatacccga aacatccggc tgtttttccg tcccgcgcgc caattcctcc
    10801 gcgtgcggcg tattgccgtc ggcagggccg ctgaaaaacc gccccgcctc ctcctgcttc
    10861 gtacggtccg caaactgccg caaagccgga tgcgactgat cgtgatgcgc cgtatcaaaa
    10921 cccgcgccgc cctgttccgc gccgccgtcc gcagccgcct gtgccgccgt ccgcagccgc
    10981 ctgtgccgcc gcacgcaacg cctgcgcccc ttccggggcc ggcctgcccg ccttcctgtt
    11041 gcccccgata cccccctgcg cacccgcctt ccgtccggcc ggcggcctga gcgggtcagt
    11101 taccgccgca taagccttcg ttccggcaaa ccccaccgca gaatgcccca aagaagagaa
    11161 agcctccaaa gccgccccct tctcgtccca ttccccgttt gccagccccg tacccaaata
    11221 ctcgcccgca aactcaccgg ccgattccgt cgcataagcc gccgcgtgcc gtgccgcacc
    11281 gcccaaaccg cccttggcag actccttcgc cagcgccgca aattcaggcg ttcccttagc
    11341 cgccgcaacc gccgccttat ccgccgccgc aacacccatt ttctccaatg cggctttccc
    11401 ggcggccttc ttgcccatat tcaaaatact gccgccaagt ttcatcgccg ccatatccgc
    11461 cgcgccgacc accgcgccct tgaccgccgc atttttcaac gcacccggcg cacctcggcc
    11521 gataaacgcc atcaccgcat ccttgtccgc agggtcgacg cccgccgcct ctgccgccct
    11581 gtccagctgc ccgccgtatt ccatcagcgt attgcccaag gtcgcgcccg cataggcgcc
    11641 gacagccgcg ttaccgcccg ccaaagcgcc caatatgccg ccggcggttg tcgtagcaat
    11701 agggacaagc gcattgggcg cctgctccag caccgcgccc cccatctcgc ccacatcttc
    11761 caaggcgccg tccaaaccct tctcgcgcca gtccttctta agctcccccc acacattcga
    11821 caggccgccg ccgacacccc cgcccttttc ccaagcctcg cccagctccc tgcgcgcttg
    11881 ggatttgcgc ccctcctgaa tcttccgata atccatatcc tcggccttga gccggcccag
    11941 ttcctctttg tcgcccgtaa gggcattcca ccccatacgc gccgaacgca tagaattttt
    12001 aaacccgcgc gtcaacgccg tatcgtcggc ggccgcccgc gcatcctttt gcgtaaagcc
    12061 gcggtactcc ttcaaacccc cggcaagccg cccgtgttcc aacggatcgt atctgaccaa
    12121 atcactcatt gctcaaccct cttctcaccc ttctcattct cataaatgat tttaccgccg
    12181 gccgttttct gaccgttcgg cttaaagccc aactgcgcat aacgcttctc gcgctctccc
    12241 gcaaactcca catccaaagg cttgcccgtc cgcaaatcga caatcccctc gcgcttcgcc
    12301 gtcaagcccg tatccgggtc ggcaacctca taactgattg tcttgaacac attcgggtca
    12361 aagcccttct ccccttgccg ctcaaacttc ccgttaagcg catcaatctg ccgcgcaatc
    12421 tccccgcgct gcccgtccga cttcgcaaaa ccataagccc gcgtcaaatc ggcgatgcgc
    12481 ttcctatcct caagctccag cgcatccgcc tcacgcttca tctcaaaccc ctgccgcttc
    12541 atccccagct cggcctgctg cacatcaaac gccctgtccg cattcctctg cgccatctcg
    12601 cggttcaacg caccctcgtt cgccttaacc gccgcatcaa accgcccctc ctgcaacacc
    12661 gcctcacggt tcgccgcatt ctccgccgca gccgccgcca gcctttccct gcccaattcc
    12721 ctgaccgccg catttttatt atgttccgcc acaatcccgc gcgccgcatt aagctgcgcc
    12781 gccgtcaact gaccgttcaa agcccccgca taaggcgtag tcgccctcct gtaaagctcc
    12841 cgctcctcag gcgacagcgc atccggcccg gcagacacca aaggacgctg cgcgtcaaaa
    12901 tcacgcaccg gtttgccgtc ccgatacaca ttcccgtcaa atcccaaagg cggcggcgtc
    12961 cgtccgcccg catccgtcgg gcgcgtatcg cgccgcgcga aatccgcccc ggcccgcgca
    13021 tcgggggaaa gcgccttatc gccgcccgaa aaccccaaac cggcatccgc accccgttcg
    13081 aaagcacccg cccggcgcaa ccgccgcgcc ccatcctcaa tattgttacg gctcaaatcc
    13141 atcacattcc gataacgcgc cggcccggcc tcataattcg caacggtacg ccgcaccgca
    13201 tcggcacggt gcgcctgtgc cgccgcccgc tcccgctcct ccgccccctt ccgggccttc
    13261 tcttttttct ccttttcctt catctcccaa aaatccccgg cagacggctc aaacaaaccc
    13321 atcgcaaacc ctccgaacaa ataaagccca aaataagcaa aaacccaaac ccaatcaaac
    13381 gcctaaagtt aagaacaaac aaaaatcgca accacaaacc aattaataaa caacaagtta
    13441 cacaaaaaaa cacaccgacg ggccatttcc gggggcaaat aaaaaccgcc ttcgtgcaag
    13501 gcggttgcaa aaaagccttt catttaacga aaggcttttt gccgcccgcg taccgcgttc
    13561 agaggttgtc cgacaccgtc ggcgtttcca tgcctgtttg ggcgtgattc gcactctttg
    13621 gcggcacggc cgtcatcggg ccggtatccg cgccgtaggc ttcagggatg ccttcgggcg
    13681 gtatttgcgt ttcaggcggc atggcgggga cggggaagcc gggatcgtcg ccccgcgccg
    13741 gcctgacata tcccgcgccc tgcatgaccg cgtcggcaac gggggcgatt tgcggcatgg
    13801 cggctatctg cccgcccgcc tgcatggccg cataagccgc ctgcacgccg atttgtaccg
    13861 cccgcgcttc gatttccttg atttcgctcg cggtacgttg ttccttgagc gccaattccc
    13921 gccgtttgat gtcgttgccg gattttgcca atgcgtcctg caccgcctgc gcgatacgcg
    13981 cctcgatttg ttcgggcgtt tcctgcactc ggacttcttt gattttttca atcactttgt
    14041 ctttaaacgg gatgtccatc agggacacca taaacggcag caccgccgcc tgatattcgg
    14101 gcggcaggga tttgaccgcc tcgctcatcg cacccagctg ctggctgcgg taggagttgg
    14161 tgctgggcac gtcttccaaa gccactttca gccgtatgtt ttgcaggtcg ttggacaaat
    14221 aagccttgcc ggttacgggg tcggtttcag gcctgttgat gacgaccgtc cgcccttgcg
    14281 tgacggcgtc cccttctatg acgacggttt gctcgtccga gcccaaatcc tcgataatca
    14341 tcgcaagcag caactcgccg accaatgagc ggctctggcg gaagttgtcc ataaccagcc
    14401 ccagcgactg gttggactgc tcgacttgga tgctttcctg cctgccgctg gtggcgttgc
    14461 cccggttgcc cataaatgac ggggtaatcc cgctgatttg ccgtattgtc gcgcggctgt
    14521 cttgaagcat ctgccaatgc tgtgccgaca attcgaaatc gcggctgacg tcgaaacgcg
    14581 cgcccggctg ggcggcctct attttgttca gcacgatgtc cgcgtccacc cgtgcgatat
    14641 tgcggcggaa ctgttcgtcc gacatatcga ctatgccttt ggtgcgtacc gtgcgtattg
    14701 ccgacaaacc ccatcgtaat ttgctgttgg tgctgttgag gttgtcctgc gcgtatttca
    14761 tattgcggac atatccgtag gggatgccgg tgttgtcctc gcggaatccg aaaaacggga
    14821 cgtaagggaa cttttgatgc ggatacgggg tcggctcgtc gcggacgacc aaatccccca
    14881 cgacaaaggc gcggcgcata cgcgggacgg aagcggcaaa caataccgcg ccgtttgccg
    14941 ccatctcccg atggtttggg tttgcgccgt caaactccac cgtgcgccct gtttttttgt
    15001 cgcgcaggca gtctgccgtt acccaccgcc tgtaccatac ttccgccacc gccagttcgc
    15061 gcgtggtttc attgaaccaa aactcttcgc tgacggtgtt gcgccccgaa ataccccaag
    15121 cgtccgccag tccggtattg ccgccgccgt caagcatttc cccgctgatg cgccagtccg
    15181 aaccgccgcg ccccatcgct ttgaagtgtc cggcatattc agggaagaat tgcgccaggc
    15241 gttccggcag cagccagcgg cggcgtatca gccagcgggc atcggacagg tcgtatttgt
    15301 aagatttcat atcccaatgg atggcgttgc ggtggatgac gccgcactca taaggaaact
    15361 cgaaagggtt ggggttgcgt gtaacctcca cccagccgat gccgcaggct atctgccccc
    15421 tgaacgcgtc cgaacaagcc ttgtcggcac ggctttgccg ttccgcgcgg ttgagtttga
    15481 agttcaatgc gtccgccacg tcccgcccgc cggtttcgcc gtccgccgtc acgcgccagt
    15541 ccgtccgtat cgtcgcctca tatccctgta ccgacagcag ggtcggggta atcaggtttt
    15601 caacggcggg gggcaggccc aactcgcgct gtttctgtaa aagctcgttg tcaagctgcc
    15661 tgccgtcggc gtagtccatt tccttgtcgg caacggcacg ccatgcaggt tggttcatca
    15721 tttcaccgac aaacagccgg tattccccga tagtcagcgg ttcgccgttt ttatcgggca
    15781 atacgcctgt ttccggtacg tccgtcccca tcaataaagc ctccaatccg aaacgggcgt
    15841 ttccgtgtat cccgtattgc tgcctgtatc cattaaatca accgcctgcg ccaggtagcg
    15901 gaacatatcc gcgccgtgcg aatattcgtc gtgcagcggc cccattgcca cgcctgtttt
    15961 cgcatgtatt tggcggccgt accgtttcag gcattccaaa agccgcgctg ttttgtcttt
    16021 atcgaagtac actttgggaa acagcatccg cgccgccctg atgccttctt cgataccggt
    16081 cgcgttttgc acgaaaaccg acttgcgccc cagtccggtc aaaatctcca tcgtactttt
    16141 gcctgtttgg aagttgcgcg tcctgccgtc gtgcggcagg aagtccgtcc cccaccggta
    16201 gggcagcttt tccaattcgg caacatacca gtccaacgtc cggtgcgtgt cttcgatgta
    16261 gccgatgatg cgcacgctcg tcaaatcgcg ctgcaccagc ccgatggtca tggcatcgtt
    16321 ccagcccaaa tcccaaaccg tatgcacggg caaagaagaa tcataaggga cgagtgtaac
    16381 gcgtccggaa tgaaaagcgt cctgtatttc atgccggtaa accgccccct ccgataccat
    16441 gcgcggcctg ccttcccaaa tattcccgta gtcctcttta ttcatcgaac gctgtgcttt
    16501 gagccgctcc cggttcaatg cttcgggaaa ccacggattg tcgcgccagt tgatttcgca
    16561 caaccaagtg tcttcggacg gcatagcgat aaaacgccgg taggtttcgt ccgtctccat
    16621 atcgggattg agggtaatcc aaatttccga accttctttg cgtatggtcg gcgtgagcac
    16681 gtcccagctt tttttactga cgccgtgccc ttcctcaacc catacgatgt cgataccttc
    16741 aaacgatttg atgctgtcca cggtatgcga ctgcaggccc gaaaacacga acagcgtgcc
    16801 gtttgcgccg cgtatctcga agtcggttat ttcgtagaaa tgccccaaac ccaactgcgc
    16861 cactttgtct ttcaacaggc ggtgtacggt atcgcgcatc gatttttgaa tttcgcgcac
    16921 gcataaaata cgcaaagggc gttgcgcccc cagcgccagc agtgcggatg ccgcgccgtg
    16981 cgatttgccg ccgccgcgcc cgccgtacat aaccttgtac cggcaaggct tgaacagccc
    17041 gtccagtttt gcgggcaatt tcaaatcaac cgtctttccc gtcatcttca ggccctacaa
    17101 attcgatacg gatgcccgcc gtcttggcgg attccgaaat cttcgattcc gcggtatcca
    17161 taccgtatga tttacgctcg atttccacca aaaccttaaa ccccgccgcc accgtttgga
    17221 acatacgcgc ctttgtgaat gtgtcctccg ggcttccgta caattccgcg acaatctgct
    17281 ccgcgctttt ggacaggttt tccgccaaat tccgatgcct gcgctgtacg gcggcaaccc
    17341 gtttcgcttc cgcgtccgtt acttgtttaa ggttgaaatc cgcataatcc ggcgcattgc
    17401 cgtttttgtc cgcggacagc accatcgccc gcgcctttgc ctgtacctcg tcattcaaat
    17461 cgcgcgtcca cacgcgttct ttggcaaccc tgccgacgtg ttgcgccgaa acattgtatt
    17521 tgcggccgat ttcggcaatg ctcaaaaccc cgcgccggta atccagctcg actttgcgcc
    17581 aatcttcatc tgacaatctc ggcattaaag gtccaacctc ccgtttttgg cggaacattc
    17641 cagataattc cgcaatactg aaaactcgcg ccccatctga atactggaaa actgctgccc
    17701 gaaccccgac atcaaagccc ccaacgagcg catttcctgc gggttgaggt gcaataccgt
    17761 atcgcctatt tccaatacgc ccatccccga atccaatacc gatacgctga cggggcgcgt
    17821 ctcgccaaac cctatggcgg gcttgaaaat cccccttgtc acgcggtaga ttttgccgtg
    17881 ctcgaccagc agcttggtaa atttagtgat ttccgacatt ttcaggcctg tcgtttccgc
    17941 caaagcctcg cggctgacca cctgctccgc gttgtgcaaa tccacaaccc cctggtacac
    18001 cttgtcaata tccgacaatc ccgacatcaa atcaaactcc taaacatccc aaacgattcc
    18061 aaactccgcc gccgcccgcg attgcaggcg gttacactac aaagccaaaa tctttagatg
    18121 ctgcctctct cgctttcact gcatcttcaa tatgttcaaa taaacctaat attttatttt
    18181 ttctattaac cataatccta gcttgccatt tcttagacgg cttatggaaa taaaccccag
    18241 tatgcccgct agtattattt ttaaacttcc ctctattttt catattttca gcatgtgtaa
    18301 catctctcaa attagatatt ctattatctg tcttatccct attgatatgg tctattgaag
    18361 aagcgtgttt cccataaaca aaaagccaaa ttaatctatg tgcattatat tgtttcttaa
    18421 atatttttaa cttcaaatac cccaacctgc ctttgtataa gaaaacctcc ttgccggaaa
    18481 atccggtatt ccaagatttg taatgcaaac cgctattgaa atactttctt ggacgctccg
    18541 cccaatataa ctttcccgta ttgtcatcat atcttaataa ttcttttaaa acctcttgtg
    18601 ttaatcccat aaaacaccat gctccataga tgcccaagat ataatctttt cttgatattc
    18661 agacatttcc cgaactgata attttgtcgt tgatataccc attaattgcc cgttaggcat
    18721 ttcaatacat ccaataaatt ttctttttaa aaattcatgc catacatctt gactaaacct
    18781 tctcccatcg ataaaaactt tttctgaaat ttcacgatac aaaaaccata gccgcctgtt
    18841 ctgctcgacg ctccgttttg acttgcgcgg gcggattgtg atttcaaggt tgccgttttc
    18901 aaaccacccg tccgggctgt cccaaatcga ccgcataacc tgctttttgt tttggggcgt
    18961 cagtgtaaat tccgccccgt tcatttcaga cggcctttca cgccgacaac ccccaatgct
    19021 tcaaggcggc gtatcgtgcg caattgcgaa cggcgcatat aaaactcttt gtcttcgcgg
    19081 cttaggcctg cgcccctgcc gtcgatcgcg ccgcggcggc aaccgcaccc gaaacccgcg
    19141 cccaaaccgc cgcttttcaa tcccgccccg tgcgtctcgc ccgggaaacg gcacaacacg
    19201 accgtttcag ggttgtcatt gcacacgccc gcgatgttcg gcgtgcattg cccgcctttg
    19261 gcggctttgc gtatcgcgct agacatccca accctcctgt ccgatgaaat cccaaccctc
    19321 gtctgccgca tccgtctgtt ccatctccaa
//
LOCUS       DAXDSD010000035        19310 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXDSD010000035 DAXDSD010000000
VERSION     DAXDSD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19310
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.035"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(78..1148)
                     /gene="lptG"
                     /locus_tag="ACFB2Q_000953"
     CDS             complement(78..1148)
                     /gene="lptG"
                     /locus_tag="ACFB2Q_000953"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC0918001.1"
                     /translation="MNLISRYIIRQMAVMAVCALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(1145..2260)
                     /gene="lptF"
                     /locus_tag="ACFB2Q_000954"
     CDS             complement(1145..2260)
                     /gene="lptF"
                     /locus_tag="ACFB2Q_000954"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC0918002.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            2249..2431
                     /locus_tag="ACFB2Q_000955"
     CDS             2249..2431
                     /locus_tag="ACFB2Q_000955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918003.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            2434..3840
                     /locus_tag="ACFB2Q_000956"
     CDS             2434..3840
                     /locus_tag="ACFB2Q_000956"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC0918004.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            3903..4343
                     /locus_tag="ACFB2Q_000957"
     CDS             3903..4343
                     /locus_tag="ACFB2Q_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC0918005.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            4603..5421
                     /locus_tag="ACFB2Q_000958"
     CDS             4603..5421
                     /locus_tag="ACFB2Q_000958"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC0918006.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGGTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGK
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            5528..6154
                     /gene="purN"
                     /locus_tag="ACFB2Q_000959"
     CDS             5528..6154
                     /gene="purN"
                     /locus_tag="ACFB2Q_000959"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC0918007.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGQIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            6170..6841
                     /locus_tag="ACFB2Q_000960"
     CDS             6170..6841
                     /locus_tag="ACFB2Q_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC0918008.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            6959..7381
                     /locus_tag="ACFB2Q_000961"
     CDS             6959..7381
                     /locus_tag="ACFB2Q_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC0918009.1"
                     /translation="MQVTSKWIDGMCFVGTAEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
     gene            7904..8620
                     /locus_tag="ACFB2Q_000962"
     CDS             7904..8620
                     /locus_tag="ACFB2Q_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC0918010.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            8769..9671
                     /locus_tag="ACFB2Q_000963"
     CDS             8769..9671
                     /locus_tag="ACFB2Q_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC0918011.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            9714..10488
                     /locus_tag="ACFB2Q_000964"
                     /pseudo
     CDS             9714..10488
                     /locus_tag="ACFB2Q_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            10600..12288
                     /locus_tag="ACFB2Q_000965"
     CDS             10600..12288
                     /locus_tag="ACFB2Q_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC0918012.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPGAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDTPVFNRTVTLKDSWQPK"
     gene            12380..13336
                     /gene="gshB"
                     /locus_tag="ACFB2Q_000966"
     CDS             12380..13336
                     /gene="gshB"
                     /locus_tag="ACFB2Q_000966"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC0918013.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKEFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GKVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            13495..13878
                     /locus_tag="ACFB2Q_000967"
     CDS             13495..13878
                     /locus_tag="ACFB2Q_000967"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFC0918014.1"
                     /translation="MEPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            14010..14483
                     /locus_tag="ACFB2Q_000968"
     CDS             14010..14483
                     /locus_tag="ACFB2Q_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC0918015.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            14608..15711
                     /gene="mnmA"
                     /locus_tag="ACFB2Q_000969"
     CDS             14608..15711
                     /gene="mnmA"
                     /locus_tag="ACFB2Q_000969"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC0918016.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            15782..17452
                     /locus_tag="ACFB2Q_000970"
     CDS             15782..17452
                     /locus_tag="ACFB2Q_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC0918017.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSAGK"
     gene            17564..19198
                     /locus_tag="ACFB2Q_000971"
     CDS             17564..19198
                     /locus_tag="ACFB2Q_000971"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC0918018.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         4734 a         5179 c         5238 g         4159 t
ORIGIN      
        1 agggaacggc tatatgtctg cgggaatgtg tcaatgaaaa caaatgccgt ctgaagagag
       61 gttcggacgg catttgttca acgtttttcc tgtttgcgta tcagccaaac agcgagcaag
      121 gcgaaggcta tggtaggcag tgcgccggcg aggaagggtg gggtgccgta gagttggctg
      181 gtaaacccga agagcctgcc ggcaaggtgg aacagcaatc cgagacagat gccgccgaag
      241 agttttaagc ccatattgcc gtggcgcgtg gtttgcggcg taaaggcgaa ggcaacgagc
      301 gccatgaccc atgcggcgac ggggtaaacg agtttacgcc accatgcgat ggcgtagatt
      361 tgggtgtttt ggctgttgtt ttggaggtgg cggatgtagg tggtcagctc gccgacggac
      421 atttggtcgg gcttgacgag caatacgtcc atcaggttgc gtctgacggc aatcggccaa
      481 gtttcttcgg cggcggcgga tgtttcgatt ttgtctgtac ccatgatgct gcggcggatg
      541 tttttcaact gccagctgcc gtcgctgttc aaaacggcgg aatcggcttc cactgcctct
      601 gccaattcgt ttttatcgtt gcgcgcccaa attttgatgc ccaaaagcgt atggtcgggc
      661 aacattccgc gcacattgat aatgctgttt ttttctttca gccaaaggcc ggtatggccg
      721 gtgctgattt tgccgttaat ggcggcggct ttgatgtttt cggctttttg gctcagcgtg
      781 ggcgcaaccc attcgccgag cgcgacggcg gcaatagcaa aaataaaacc gaactgagac
      841 agaatcaaca gcagcttttt ggtgctcatg ccgctggctt tgatgacggc cagttcgctg
      901 ccggcggcaa gctggctgag agaggccagt ccgccgatga ggacggcgag gggcatgagt
      961 tcgtaggcgc gggcgggcat tttgagggcg gtgtagccca gcatttccca tatgccgtaa
     1021 ctgcctttgc cgaggttgcc ggtttcgtac aggatttcaa aaaagctgta caaagcgagg
     1081 aaggcaagga gcgcgcaaac cgccataacc gccatttggc ggatgatgta acgtgaaatc
     1141 aggttcattt tccgcctttc aatgtcagac ttttgccaac cgcctgccag aagggctggc
     1201 tgggcatact gcggacgcgc agaagtacga ttgcgatgac gaacatgatg atgtgcatag
     1261 gcagcagtcc gagccaaaaa tggattttgc cgtcttccac ggcttcaaaa agcagggtca
     1321 gcccgttttg gtaaattaaa aacaaaccga tggcaatcaa gatattgtag gtatgtccgc
     1381 tgcgcgggtt gaaataggaa agcggcacgg cgagtaggca gagcaggagg acgctgacgg
     1441 tcagcgagat gcgccacatc aattctgcct gatgttgcgg attgctgctg ccaatcagtt
     1501 gggcggtcga aatggtgcgg cggtgggaaa cggggtcgat aagtttgggc gtggtgctga
     1561 taatcaggtt gagtttttgg aaggaaacct gattgtagtc ggcgcgtccg ggcgtgccgc
     1621 tgtaacggta gccgtggcgc aattcgagcg tgcgtttgtt gtcgttcagc gagaagttac
     1681 cttctttggc gaagatgatg ttgtcgccgc cgtttttgtc ctgttcgcgc aggaacaggt
     1741 ttttcatgat gccggattcg gtgtcaaagg tttcgacgaa ataaaccctg ccgttgcgct
     1801 tgcccaagtt attgaactcg ccggcttcca ccaaagacaa ttcctgcttc tgcttcaaaa
     1861 tttcggcata ttcgcggctg cgcagttccg cccacggtat cacccaaagc tgcatgacgg
     1921 caatcaggat ggcaaacggc acggcaaact gcatgacggg gcgtatccac tgtttcaacg
     1981 ccaatccgca ggatagccag accgacattt cgctgtcgcg ccagtagcgg gtcaatacgg
     2041 tcagcgtgct gatgaatgcg gtcaacacca gcaaaagcgg ggtcataccg atgacccaga
     2101 agccgactaa ggccaacacg gcatcgatgg cgacacgccc gtcagctgcg cggccaagca
     2161 ggttgatcgc ctgcgtggac accaacaccg ccaagaggac gacgaaaatg ccgacggcgg
     2221 taaaagagag ttctttgatg aggtttcttt gataaatcat aaaatcgcgg tcaggggggc
     2281 ggtaagcggt tcggcgcagg cggattgtcc gcgcaaggtt tcagacggtg cttcaaaaaa
     2341 tggggtacaa tcgtgccagc cgtcttcaga cggtcttttg agcgaaaggc cgtctgaaaa
     2401 ctaccgacaa ccgtctcaat caggagaata aacgtggaat ttagcacaaa aaccgaaatc
     2461 ttgcaggaac agcaggcagg cgcgcagtta tttgtctgtg ccgacaaagc acccgagcac
     2521 aacaccgccg cacatgcgct cttctctgct ttggaagagg gtcagaattt ttccgacacc
     2581 aaaatcccga cggacaacgg tttgcaggca gtcgccgtcg tccgcctcga aaaaaccgac
     2641 cgcgccgcgc tgaacaaagc cgccgccgaa gccgccaaat gggcgcaaaa tcaagaaacg
     2701 gtcaatgtgg acgttcacgc ctttgatgag gcgcaagcag cagccgttgc cgaagctttt
     2761 gccatcgcgt tcggcaacgc tgcatatcgt ttcgaccgct acaaaaaaga agccaagccc
     2821 gccaaatttt cgcaagccgt gttccacagc gcacacgaag ccgccgtcaa agaagccctg
     2881 cgcgttgccg aagcacaagt ttacggacaa agcctctgcc gcgacttagg caatgccgca
     2941 ccgaacgaat gcacgcctga gttcctcgcg cgtaccgcca aagccgaagc cgagaaactg
     3001 ggcgcgcacg ccaaaatcat cgaaaaagac tacatcaaag aaaacatggg ttcgttttgg
     3061 tcggttgcca aaggcagcgt cgaagaccca tatttggttg aactgagcta tttcggtgcg
     3121 gccgacaaag aagccgcgcc tgtggtattg gtcggcaaag gcattacctt cgacaccggc
     3181 ggcatttccc tcaaacccgg cctgaacatg gacgaaatga agtttgacat gtgcggcgcg
     3241 gcaaccgtca tcagcacctt ctgcgccgcc gtcaaactgc aactgccgat caatctgatt
     3301 gccatcgtcg ccacttgtga aaacatgcct tccggcgcgg caaacaaacc gggcgatgtt
     3361 gtgaaaagta tgaaagggct gacgattgaa gtgttgaaca ccgatgccga aggccgtctg
     3421 attttgtgcg acgcgctcac ttacgccgag cagttcaaac ccaaagccgt catcgatgtc
     3481 gccaccctga ccggcgcgtg catcgtcgcc ttggggcacg atgtcagcgg cgtgatgggc
     3541 aataatcagg atttgatcga cagcctgctt gccgcttcct acaacgtgga cgacaaagcg
     3601 tggcaactgc cgctctttga aacctacaaa gaccagctca aatccaactt tgccgacatc
     3661 cccaatatcg gcacgcccgg cgcgggcacg attaccgccg caacattcct gtcctatttc
     3721 accgaaggct acccgtgggc gcaccttgac atcgcgggta cggcgtggaa atccggagcg
     3781 gaaaaaggtg cgaccggccg ccccgtaccg ctgctgatga actatctgcg gaatctttaa
     3841 ttttgccaca atgccgtctg aagcttttca gacggcatca tcctgtcaaa tgctcaaaca
     3901 atatgccgaa agttaccttt tatacccatg ttgaccaaat cccccttttt acctgtcggt
     3961 tgattgcgcg cgctatccga gacggcggca ggatactggt gtggtccgac tcgttcgggc
     4021 ggcttcagga attggacaaa atgctttggc aatacgaggc cgagagtttt attccgcacg
     4081 aaatttggga aacggaagaa gccatgccgt ctgacacatc cgtcctgctt gcctgcggcg
     4141 gcaatctgcc ccgaattccc gaaggcatgg ccgttttgaa cctgtccgac ggtttttgga
     4201 acaccgcttc ggtcttgccc gcgcgcgttt tggaaatcgt cggcaacagc ctggaagatc
     4261 tcgccgacgc gcgcgaacgc tttaccgcct accgccgaag cggttttgcc atcgaacatc
     4321 acggcatgga gggcaaggca tgaaatgccg tctgaaatgc cgcatttaca aaatttaaac
     4381 aggcttgatt ctatttgatt aaaatttcct tatcggttga accccgccac ttggacatct
     4441 gtccttcggg gcggtagaat caaaccttat tttggaaggt tcaatccctt ccaaaacagg
     4501 gcaacacaca gattgacgct ttatgtgcca tcctgtgtgt tgaaacattc aaactcggct
     4561 ataatcccgt ttttccgact ttatcgacag cgaagatcca tcatgaacac cattttcaaa
     4621 atcagcgcac tgaccctttc cgccgctttg gcgctttccg cctgcggcaa aaaagaagcc
     4681 gcccccgcat ctgcatccga acctgccgcc gcttctgccg cgcagggcga cacctcttca
     4741 atcggcggca cgatgcagca ggcaagctat gcgatgggcg tggacatcgg acgctccctg
     4801 aaacaaatga aggaacaggg cgcggaaatc gatttgaaag tctttaccga tgccatgcag
     4861 gcagtgtatg acggcaaaga aatcaaaatg accgaagagc aggcccagga agtgatgatg
     4921 aaattcctgc aggagcagca ggctaaagcc gtagaaaaac acaaggcgga tgcgaaggcc
     4981 aacaaagaaa aaggcgaagc cttcctgaag gaaaatgccg ccaaagacgg cgtgaagacc
     5041 actgcttccg gtctgcagta caaaatcacc aaacagggta aaggcaaaca gccgacaaaa
     5101 gacgacatcg ttaccgtgga atacgaaggc cgcctgattg acggtaccgt attcgacagc
     5161 agcaaagcca acggcggccc ggccaccttc cctttgagcc aagtgattcc gggttggacc
     5221 gaaggcgtac ggcttctgaa agaaggcggc gaagccacgt tctacatccc gtccaacctt
     5281 gcctaccgcg aacagggtgc gggcgaaaaa atcggtccga acgccacttt ggtatttgac
     5341 gtgaaactgg tcaaaatcgg cgcacccgaa aacgcgcccg ccaagcagcc ggatcaagtc
     5401 gacatcaaaa aagtaaatta agtccgaatc catgcccgaa acaggttttc gggcattttt
     5461 acggcaaatg ccgtctgaag ccaccaaaca gcggttcaga cggcatccct ttcaaaagcg
     5521 caccattatg aaaaacatcg tcatcctgat ttccggacgc ggcagcaata tgcaggcaat
     5581 cgtcaatgcc gccattccca acgtccgcat tgccgccgtg ttgagcaaca gcgaaacggc
     5641 tgccggtttg caatgggcgg ccgaacgcgg catcccgacc gacagcctga atcataaaaa
     5701 ctttgaatca cgacttgcct tcgatacggc catgatggaa aaaatcgacg catatcaacc
     5761 cgatttggtg gttttggcag gttttatgcg gattctgacc cctgagttct gcgcccatta
     5821 cgaaggcagg ctgatgaaca tccatccgtc catcctgccc tcgttcaccg ggcttcatac
     5881 gcacgaacgc gctttggaag caggttgccg cgttgccggc tgtacgattc atttcgttac
     5941 ggccgagctg gattgcggcc agattgtatc gcaagggatt gtgccgatac ttgacggcga
     6001 tacggcagac gatgttgccg cccgggtttt ggctgtcgaa cacaaactct atccgaaagc
     6061 cgttgccgat tttgccgccg gccgcctgat tatcgaggga aaccgtgtca gaaattcgga
     6121 aaacgccgat gccgcccgtt tcctgacggc gtaaaccggg cgggagcaaa tgatgaagac
     6181 ttttaaaaat atattttccg ccgccatttt gtccgccgcc ctgccgtgcg cgtatgcggc
     6241 aaggctaccc caatccgccg tgctgcacta ttccggcagc tacggcattc ccgccacgat
     6301 gacatttgaa cgcagcggca atgcttacaa aatcgtttcg acgattaaag tgccgctata
     6361 caatatccgt ttcgagtccg gcggtacggt tgtcggcaat accctgcacc ctgcctacta
     6421 taaagacata cgcaggggca aactgtatgc ggaagccaaa ttcgccgacg gcagcgtaac
     6481 ctacggcaaa gcgggcgaga gcaaaaccga gcaaagcccc aaggctatgg atttgttcac
     6541 gcttgcctgg cagttggcgg caaatgacgc gaaactcccc ccgggtctga aaatcaccaa
     6601 cggcaaaaaa ctttattccg tcggcggcct gaataaggcg ggtacgggaa aatacagcat
     6661 aggcggcgtg gaaaccgaag tcgtcaaata tcgggtgcgg cgcggcgacg atacggtaac
     6721 gtatttcttc gcaccgtccc tgaacaatat tccggcacaa atcggctata ccgacgacgg
     6781 caaaacctat acgctgaagc tcaaatcggt gcagatcaac ggacaggccg ccaaaccgta
     6841 aatcggtttt caggcggttt tttgccgttt tgtccatatt tctgtataag ccgcttgaaa
     6901 ttgtttacaa taacgccata tgcaaagcgg ttttaacgct attttcggga atgacaccat
     6961 gcaggttaca tcaaaatgga tagacggaat gtgttttgtc ggcacggcgg aaggcgggca
     7021 cagcgtcgtt atggagggat cggcggcaga aggcgcggcc aagcgcgggc ccagcccttt
     7081 ggaaatgctg ctgttggggg tggcgggctg ttcgagcatc gatgtggtga tgattgccga
     7141 aaaacagcgt cagaaagtga ctgactgccg tgcgacggtt acggcgaaac gggcggacga
     7201 tgcgccgcgc gtgtttaccg aaatccacat ccatttcaag gtaatcgggc atgatttgaa
     7261 agaatcggcc attgagcgcg ccgttcagat gtctgccgaa aaatactgtt cggcttcgat
     7321 tatgttgggc aaagcggcaa agattaccca cagttttgaa attgccgggg cggagaaata
     7381 ggcatcgggc ggcattatgg ggaaacggct gatgccgtct gaaggggaaa ggtgcggcag
     7441 gcaatccgga ggcgcataaa aaagcagata tattcggact gcacctcccg aatatatctg
     7501 cctgctgttt cctctttatt cagcctttat aatacttgga cttgtcgggg tattgtgcag
     7561 gcttgattcc ggattgtcaa caattttcgg tcaaatttta aatgccgcgt tttaaaatga
     7621 tgcccgcctg attttgcggg cgggcggaag gcgggaatcc cgttatttta agccatatat
     7681 taatttattg aattaaaata aatttgtgca atatagtgga ttaacaaaaa ccagtacggc
     7741 attacctcgc cttgccgtac tatttttgta ctgtctgcgg cttcgtcgcc ttgtactgat
     7801 ttaaatttaa tccactatac tttaatttct cctacacgat ttgaaacaca gccgtcttaa
     7861 aacatcttaa aaaatttgaa agcgtaaaaa tacggaaaac atcatgaact ttgaaaacga
     7921 cgacattatc catgcgccga ccacgtcttc cctgattctc gaagagcggc acgattcgga
     7981 gctgttccgt gtttacgccc tgattttgga cggcattacc gatcaggtgc tgctgcccgg
     8041 caaaaagctg accgaatccg aactttgccg tcagatggtg tgttcgcgca acaccgtccg
     8101 cggcgcgctg tcgcttttgg cgcacgacaa gattgtcgat ttgcaaccca acaggggcgc
     8161 gttcgtccac gttcccgatt tgaaagaaat gcaggatgtg ttcaatgcgc gcatcgaaat
     8221 ggagacgatg attttgaaca tcctcgccgg cctgccggat ttggaaacgc gcctcaagcc
     8281 gctttatgcg atgataaggt gcgaagaaga ggcctccggc aggggcgacc gcgtcggctg
     8341 gaaccgcctg tccaatgcct tccacgtcga actggcgcgc ctggtgggca acgatgtgct
     8401 gttcgacatt atgaacacgc tgtgcgcgcg gtcttccctg attgtcgccg tggcgggcgt
     8461 gcatcgcgag aaaaaacacg ccatcaatac gcatacgcat tccgaacacc gcgaaatcct
     8521 cgacctgctg ctggcgggca ggcgcaaccg cgtggtcaaa atcctgcgcc gccatttggg
     8581 caactgtatg gagcgtttgg aaaagacttt ggaagattga atgccggagc ggataagccg
     8641 cctgaacctt caggcggttt tttaatggcg aacctgatgc cgtctgaaat atggatgcgg
     8701 gtatctgcaa ttttcagacg gcaattttta agccgcacat atcatgcggc aataaaggag
     8761 ggtaggggat gagcagcctg atgacgttgt tttcggtatt ggtaccgatg tttgccggat
     8821 tttttatccg tgttcccaag ccttacctgc ccgcttcgga caaggtgctg tcggttttgg
     8881 tgtatgccgt gctgctgctg atcggcgtat cgttgtcgcg cgtggaggat ttgggttcgc
     8941 ggttgggcga tatggcgttg acggttctgt ggctgtttgt ttgtacggta ggggcgaacc
     9001 tgcttgcctt ggcagtgttg ggaaagttgt ccccgtggcg gataggggga aaagggaagg
     9061 gcgtttcggt cggcgtgtcg ggcagtgtga ggcagctcgg atgcgtactg atcggttttg
     9121 tgtccggcaa attgatgtgc gatatttgga tgccgtctga aaacgcgggt atgtactgcc
     9181 tgatgctgct ggtgttcctc atcggcgtac agctcaaaag tagcggcgta tcgttgcggc
     9241 aggttttgct taaccggcgg ggcatccggc tgtcggtttg gtttatattg tcatctcttt
     9301 caggcgggct gctgtttgcc gcatcggcag atggtgtgtc gtggacgaaa ggtttggcga
     9361 tggcttccgg cttcggttgg tattccctct cgggtttggt aatgaccgag gcttacgggg
     9421 cggtatgggg cagcatcatg ctgctgaacg atttggcacg agagctgttt gcactggcat
     9481 ttattccgct gctgatgaag cgttttccgg atgcggcggt gggggtcggc ggcgcgacca
     9541 gtatggattt cacattgccc gtaattcagg gtgcgggcgg tttggaagtc gtgccggtag
     9601 cggtcagctt cggcgtggtg gtcaatatcg ccgccccgtt tctgatggtg gtgttttcca
     9661 cgctgggctg aacgcggtaa aatcggcatc ccgatgcaag gaagcagaaa acgatgaaac
     9721 cgaaaatcca aaggcatgga gagattttaa gccttgtccg ccggcatcag tttatgtcgg
     9781 tggacgagct tgccgccgca ttggacgtta ccccgcagac gatacgttgc gacatccgcg
     9841 agttggagga gggcggcagc ctgaaacgcc atcacggcgg cgcatcttcg ggcggaaact
     9901 tgccggaggg tctgcccgcc gaccgccaaa cccggtgtca aaacgaaaaa aacgccattg
     9961 cccggctgat tgcggaacac atccccgacg gttcgtcgct gttcgtcagt atcggtacga
    10021 ccatggaagc cgcggcatca gagctggtga agcggcgcag cagcctgcgg attattacca
    10081 acaatatcca cgtcgcctcc gtcgtttcgg cacgtacgga ttacacggtc atcatcacat
    10141 ccggcgtcgt ccgccctttg gacggcggta ttaccggcgt ggcgaccgtc gactttatca
    10201 accagttcaa agtcgattat gccgtgatga gcacgcacgg cgtggaaagc gacggttcgc
    10261 ttttggatta cgattacaag gaagtcagcg tcatgcaggc gatgattgcc aacgcgcgcg
    10321 tccgttttct cggcgtggat cacagcaaat tccgcagcaa cgcgctggtc aggctcggcg
    10381 gcattaccgg cgtttgacaa agtatttacc gacaggttgc ctgataccgc gatgcagaag
    10441 atgctgaaag aggcgggggt ggaatgcctg attgccgatg ccgtctgaac gctatgtcaa
    10501 agcgcgcaag tcgggtacaa taaacacatc atcaaaccgc ttcagacggc atacggaacc
    10561 tccccaatgc cgtctgaagc catctgttta aagaaaacca tgctcaataa agaccaattc
    10621 gcggacaacc atttcatccg caccatcatc gaagacgacc tcaaaagcgg caaacacgaa
    10681 gccgtccaaa cccgttttcc gcccgaaccc aacggctatt tacatatcgg acacgccaaa
    10741 tccatctgcc tgaacttcgg tttggcgtat atttacgacg gtttgtgcaa cctgcgtttc
    10801 gacgacacca acccggaaaa agaaaacgac gaatacgtca acgccatcaa agaagacgtc
    10861 gagtggctcg gtttccattg ggcgggcgaa ccgcgtttcg cttccgacta tttcgaccgg
    10921 ctttatgact acgccgtcgg tttaatcaaa gacggcaaag cgtatgtcga tgatttgacg
    10981 cccgaagaaa tgcgagaata ccgcggtacg ctgaccgaag cgggtaaaaa cagcccttac
    11041 cgcgaccgca gtatcgaaga aaacctcgac ctgttcacgc gtatgaaaaa cggcgagttc
    11101 cccgacggca gcaaaaccct gcgcctgaaa atcgacatgg cggcaggcaa catcaatatg
    11161 cgcgatcccg tcatctaccg catccgccgc gcccatcacc acaacaccgg cgacaaatgg
    11221 tgcatctacc cgatgtacga ttacacgcat tgcatttccg atgccatcga aggcatcacg
    11281 cattccttgt gcacgctcga atttgaagcg caccgtccgc tttacgactg ggtgttggac
    11341 aacatccccg cgccgcacgc cacccgtccg cgccaatacg agttttcccg tttggagctt
    11401 ttgtacacca ttacctccaa acggaaattg aatcagttgg ttgtggaaaa acacgtttcc
    11461 ggctgggatg atccgcgtat gccgaccatt tccggtatgc gccgccgcgg ctacacgccc
    11521 gaaggggtgc gcctgtttgc caaacgcgcc ggtatttcca aatctgaaaa catcgtcgac
    11581 atgagcgtgt tggagggtgc gattcgcgaa gagctggaaa actccgcccc gcgcctgatg
    11641 gcggttttga acccgctcaa agtgaccctg accaactttc aagccggcaa aacccaaagc
    11701 cgccgtgccg cgttccatcc gaaccacgaa gaaatgggcg atcgcgaagt acctgtttca
    11761 caaaccatct acatcgaagc cgacgacttt gccgaaaatc cgcccaaagg cttcaagcgt
    11821 ctgattcccg gcggcgaagt acgcttgcgc cacggctatg tcatcaagtg cggtgaagtc
    11881 gtcaaagacg aagcaggcaa tgtggttgaa ctcaaatgca gcatcgacca cgacaccttg
    11941 ggcaaaaatc cagaaggccg caaagttaaa ggcgtgattc actgggtttc cgccgaacac
    12001 gccgccgaaa tcaaagtccg tctgtacgac cgcctcttta ccgtcgagcg tcccggtgcc
    12061 gtgcgcggcg aagacggcga atacctgccg tttaccgatt tcctcaatcc ggaatccgtt
    12121 aaggaaatca ctgcttacgc cgaacctgcc gcgaaagatt tgccggcgga aagccgttgg
    12181 cagttcgagc gcatcggcta ttttgtgacc gaccgccaag accacggcaa agacacgccg
    12241 gtgtttaacc gcacggtgac gttgaaagat tcttggcagc ctaagtaaac cccatccttg
    12301 ccgtctgaat attgttcggg cggcatttct cctttacccg cgaaatgcgg cacattcggc
    12361 acaccggaaa ggaaatatga tgaaagtcct ctttatcgcc gacccgatgg caagtttcaa
    12421 aacctacaaa gacaccacct acgcgatgat gcgggaaatg gcaaaacgcg gctggcggct
    12481 gtttcatacc ttgagcgggg aattgtctgt aaacggcggt ttggtaacgg cacaggcatc
    12541 ggcatttgaa ttttcgggtg caaaaaacga tgatgaccat gaatggttta aagcggcgga
    12601 caaagttcag acggcattaa aagaatttga tgccgtgatt atgcgtaccg atccgccgtt
    12661 cgatatgcaa tacctttact ccacccaatt actgacgctg gcggaacagc agggcgcgaa
    12721 agtgttcaac agcggacggg cgatgcgcga ctttaacgaa aaactggcga ttttgaattt
    12781 cagccgcttt accgcgccca cgctggtaac gacccgttcc gccgatgtcc gcgcattttt
    12841 gaaagaacac ggcgacatca tcgtcaaacc gctcgacggc atgggcggca tgggcatctt
    12901 ccgcctgacc gaaaaagacc ccaacatcgg cagcatcctc gaaaccctga tgcggtttga
    12961 ttcccgcacc attatggcgc aacgctacat tcccgaaatc gtacacggtg acaaacgcat
    13021 cttgattatc ggcggcaaag tcgtccccta tgctttggcg cgtatcccgc aaaacggcga
    13081 aacacgcggc aatctggcgg caggcgggcg cggtgtggcg caggaattgg acggacgcga
    13141 ccgggaaatt gcagagactc tggctcccga gcttaaacgg agcggcatcc tgctggccgg
    13201 tttggacgtt atcggcagca acctgaccga agtcaacgta accagcccga ccggattcca
    13261 agaaattatg aaacaaaaaa gtttcgacgt ggcggcaatg tttgccgatg ccgttgccgc
    13321 gtggtcggta cgttaaaccg atgccgtctg aaaggctttt gcttcgtaac cgcttggttt
    13381 gttcgggcag gcagggtttt ccctcggccg gcagggatgc gcttaatgcc gtacggacac
    13441 ccctccgccc cgttttcaga cggcatatat tgaggacatt ttgaaaggat accgatggaa
    13501 ccttcctcct acgcggcaga aaaaaaaggc aaaagcggca tcaggcgcgt catcaacgca
    13561 ttcggctatt cgatagacgg catcgccgcc gcctaccgtt acgaagcggc attccgtcag
    13621 gttttgtggc tgaacgcgct gctggtgtgc gcggcatttt tttgggtttc cgaaaccgcc
    13681 gtccgcctgc cgttgattat cgcgtctttt gtgtcggtca ttgtcgaact gttcaacacc
    13741 gccgtcgaag ccgccgtcga tcatacttcg accgaaaaac acgagctggc caaacgcgcc
    13801 aaagatgcag gttctgccgc acaactggtt gccatgctga tgttggcggc ggtttggctg
    13861 tccgccctgt tcgggtaaaa cgcttgcagc aggatttgta atcctttagg attagtattt
    13921 attattcact taatctacat caaatttccg agcagtattt gtatgtaaga ttaagcacat
    13981 tccccgtctg atattaaagg agcaggaaga tgaaaaaatt attggcagcc gtgatgatgg
    14041 caggtttggc aggcgcggtt tccgccgccg gagtccatgt cgaggacggc tgggcgcgca
    14101 ccactgtcga aggtatgaaa atgggcggcg cgttcatgaa aatccacaac gacgaagcca
    14161 aacaagactt tttgctcggc ggaagcagcc ccgttgccga ccgcgtcgaa gtgcataccc
    14221 acatcaacga caacggcgtg atgcgtatgc gcgaagtcaa aggcggcgtg cctttggagg
    14281 cgaaatccgt taccgaactc aaacccggca gctatcacgt gatgtttatg ggtttgaaaa
    14341 aacaactgaa agagggcgac aagattcccg ttaccctgaa atttaaaaac gccaaagcgc
    14401 aaaccgtcca actggaagtc aaaaccgcgc cgatgccggc aatgaaccac ggtcatcacc
    14461 acggcgaagc gcatcagcac taatctgctg gaaatatttg aaatgccgtc tgaaaaagcc
    14521 cgggctttca gacggcattt ttatgcccgc ctttaaaatg tgttaaaatc cgcctttaaa
    14581 aaccgccgtt tccaagccat cctgcgtatg aatacgacat caaacacttc caatatcatc
    14641 gtcgggcttt ccggcggtgt cgattcttcc gtaaccgccg ccctgctcaa gcagcagggt
    14701 tatcaagtgc gcggtgtgtt catgcagaac tgggaaaacg acgacaacga cgaatattgc
    14761 agcatcaaac aggattcgtt cgatgccatc gccgttgccg atattgtcgg catcgacatc
    14821 gacatcgtta atttcgccgc gcaatataaa gacaaagttt ttgcttattt tcttcaggaa
    14881 tacagtgcgg ggcgcacgcc gaatccggat gtgttgtgca atgccgaaat caaattcaaa
    14941 tgctttttgg actacgccgt agggcagggc gcggatacca ttgccaccgg acactatgcg
    15001 cgcaaagaag tccgcaacgg cgtgcattac ctgctcaaag gtttggatcg aaacaaagac
    15061 caaagctatt ttctctaccg cctcaagcct ttccaactcg aacgcgcgat ttttccgttg
    15121 ggcggtttgg aaaaacccga agtgcgccgc cttgccgccg aatttaattt gccgactgcc
    15181 gctaaaaaag acagtaccgg catctgtttc atcggcgagc gtccgttccg cgagtttctg
    15241 cagaaatact tgccgaccga caacggcaaa atggtgacgc ccgaagggaa aaccatcggc
    15301 gaacacgtcg ggctgatgtt ttacacattg ggtcagcgca aaggattggg catcggcggc
    15361 gcgggcgaac cgtggtttgt tgcggctaaa gatttgacga aaaacgaact catcgtcgta
    15421 caaggacacg accatccgct gctctatacc cgcagccttg tgatgaacga tttgagtttc
    15481 acgctgcccg aacgtccgaa ggcaggacgc tatacctgca aaacgcgtta ccgtatggcg
    15541 gacgcgcctt gcgaattgtg ctatttggat gatgaaaccg ccgagctggt gtttgacgaa
    15601 ccgcaatggg cggttacgcc gggtcagtcc gccgtgctgt acgacgtcga catctgtttg
    15661 ggcggcggca tcatccaaac gaccgacaaa cccgtcatca tcacgcgata aaggtaatgc
    15721 cgtctgaaac ggttttcaga cggcattgtt ccgctcaatt ccactttaaa gaccgatacc
    15781 tatggaaaaa atctggttag aaagctacga gaagggcgtc agtgccgaaa tcgacatcac
    15841 gcaatacaat tccgtcagcg acgtattccg ccaaagcgtg gaaaaatttg cccgtctgcc
    15901 cgcttttcaa aatatgggca aaacgctcac ttatgccgaa accggcaaac tggcgaccga
    15961 tttcgcctct tatctgcaaa acgtcctcaa gctgccgcgc ggcgaacgtg ttgccattat
    16021 gatgccgaac gtattgcaat atccgattgc ccttttcggt attttgcagg caggtttggt
    16081 ggcggtgaac accaatccgc tctatacgcc gcgcgagttg gagcatcagc tgaaagacag
    16141 cggtgcgacc gccatcatcg ttttggaaaa tttcgccaac acgctggagc tggtgctgcc
    16201 gcgcacgcag atcaaacacg tcatcgtcgc ctccgtcggc gaaatgttcg ggctgcttaa
    16261 aggttcgctg atcaatttca tcatccgaaa aatcaagaaa atggttcccg aataccgtat
    16321 tcgggaaacc gtttcctttc agacggcatt gaaagagggg gcgaagcacg ttttccaacc
    16381 tgtcgcatta aaccgcgaag ataccgcatt gttgcaatac acgggcggca cgacaggcgt
    16441 tgccagaggc gcggtgctga gccacggcaa catctgcgcc aatatgcttc aggcaaaaga
    16501 atggattaaa aaccaattgc gcgagggaaa agaaaccgtt atcgccgccc tgccgctgta
    16561 ccacatcttc gccctgacag tgaatctgat gatttttgcc aatgccggct cgaaaattgt
    16621 cctgattgcc aacccgcgcg atatgaaagg ctttatcggc gaactgaaaa agcagcgggt
    16681 taacgtattt atcggcgtga acacgctgtt taacgcgatg gtcaaccggc ccgatttcgc
    16741 cgaagtcgat ttttcaggat tgcggctgac tttgggcggc ggtatggcga cccaaaaagc
    16801 cgttgccgaa aaatggaaaa aaatcaccgg cacgcccatc gtcgaagcct acggtttgac
    16861 cgaagccagc cccggcgtgt gctgcaaccc cttaaacatc gaatcataca gcggcagcat
    16921 cggtttgccc gtcccgtcca ccgaagtcga actgcgcgac gcaaacggca aagaagtccc
    16981 cgttgggcag ccgggcgaat tgtgggtaaa aggcccgcaa gtgatgcaag gctactggaa
    17041 ccgccccgaa gaaaccgcca aagccataga cgcgtgcggc tttttggaaa ccggcgatat
    17101 tgccgtgatg gacgaaaaag gccgtctgaa gctggtcgat cgcaaaaaag acctcgtcgt
    17161 cgtttccgga ttcaatgttt atccgaacga aatcgaggaa ttcatcgcgc accacgaaaa
    17221 agttatggaa gttgcgtgta tcggcgtacc cgacgaaaaa accggcgagg cactcaaagt
    17281 gttcgtcgtc aaaaaagacc cgtctttgac caaagaagaa ctcaccgctt tctgccgtac
    17341 cggtctgacc gcatacaaag tgccgaaaga catcgaattc cgcgacgagt tgcccaagtc
    17401 caatgtcggc aaaatcctgc gccgcgagtt gcgccaaagt gccgggaaat aaagaaaaga
    17461 taccgtctga aaacagccgt ccaccgttca gacggcatcc gtccgtttgc aagaaccgcg
    17521 cgcttcacgt taaaatcacg cattccaata cgggtattcc atcatgacca aattcatttt
    17581 cgtaaccggc ggcgttgtct cctcactggg taaaggtatc gccgccgctt ctattgccgc
    17641 catcctcgaa tcgcgcggct tgaacgttac catgctcaag cttgatcctt atatcaacgt
    17701 cgatcccggc acgatgagcc cgttccaaca cggcgaagtg ttcgtaaccg acgacggcgc
    17761 ggaaaccgac ctcgacttgg gacactacga acgtttcatc gattccacga tgacccgccg
    17821 caacagcttc agcacgggtc aggtgtacga aaacgtcatc gccaaagaac gacggggcga
    17881 ctatttgggc ggcacggttc aagtcatccc gcacattacc gacgaaatca aacgccgcat
    17941 ccacgaaggc gcggcgggtt acgatgtggc gattgtcgaa atcggcggta cggtcggcga
    18001 catcgaatcg ctgccgtttt tggaagccat ccgccagatg cgaagccagt tgggacgcaa
    18061 caacaccctg ttcgcccact tgagctacgt tccctacatc gccgccgcag gcgaaatcaa
    18121 aaccaagccg acccagcaca ccgtgaaaga aatgttgagc atcggcttgc aacccgacat
    18181 cctgatttgc cgtatggaca ggaaaatgcc ggcagacgaa cgccgcaaaa tcgccttgtt
    18241 ctgcaacgtg gaagagcgcg cgattgtcgg cagctacgat gtggacagca tctacgaatg
    18301 ccccgaaatg ctgcacgacc aaggcatcga caacatcatt accgagcagt tgcagcttaa
    18361 cgtgcagcag gcggatttga ccgcgtggaa aaaaatcgtc cacgccgtca aaaacccgaa
    18421 acacaccgtc aaaatcgcga tggtcggcaa atacgttgat ttgaccgaat cctacaaatc
    18481 attgattgaa gccttgaaac acgcgggcat ccataccgaa accgatgtgc agattacctt
    18541 cgttgacagc gaaagcatcg agaaaaacaa gggcgacgtt tccgtactca aagatatgga
    18601 tgccatcctc gttcccggcg gcttcggttc gcgcggcgtg gaaggcaaaa tcgccgccgt
    18661 gcgctacgcc cgtgaaaaca acgtgccata cttgggcatc tgcctcggta tgcagattgc
    18721 gctgattgaa tacgcccgcg acgtggcagg tttgaaaggt gcgaattcca ctgagtttga
    18781 cttgaaatgc gctgcccccg tcgtcgccct gattgacgaa tggcaaaccg ccgacggcag
    18841 cgtcgaaacc cgtgacgaat ccgccgattt gggcggcacg atgcgtttgg gcgcgcaaga
    18901 agtcgaattg aaagcaggca gcctcgccgt caaaatctac ggcagcggac acattcgcga
    18961 acgccaccgc caccgctacg aagtcaacaa caactacgtt tccgcgctgg aacaggcagg
    19021 tttggtcatc ggcggcgtat ccgccggacg cgaacgcttg gtcgaaacca tcgaactgcc
    19081 gaaccatcct tggttcttcg cctgccagtt ccatcccgag ttcacgtcca acccgcgcaa
    19141 agggcatcct ttgttcaccg cgtttgtcaa agccgcgttg aacaataaaa aagcctgata
    19201 aagcgttact tgatgatcaa aatgccgtct gaaagcctga caaagacttt cagacggcat
    19261 tttcgcaaat cggggatcat aaaagccatc aatttaattt gggtctgtac
//
LOCUS       DAXDSD010000036        19294 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXDSD010000036 DAXDSD010000000
VERSION     DAXDSD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19294
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.036"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            397..1239
                     /locus_tag="ACFB2Q_000972"
     CDS             397..1239
                     /locus_tag="ACFB2Q_000972"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC0918019.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1268..1732
                     /locus_tag="ACFB2Q_000973"
     CDS             1268..1732
                     /locus_tag="ACFB2Q_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC0918020.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETV
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     ISSSELVEIVLEELFGQNTEAAVRFAALHKRFDSPEHFASWLAQAVKTGGKT"
     gene            1764..2873
                     /gene="ribD"
                     /locus_tag="ACFB2Q_000974"
     CDS             1764..2873
                     /gene="ribD"
                     /locus_tag="ACFB2Q_000974"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC0918021.1"
                     /translation="MFSDTNISMMENALRLAALGRFSTSPNPRVGCVIAHGRQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGGGKDLFSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            3068..4489
                     /locus_tag="ACFB2Q_000975"
     CDS             3068..4489
                     /locus_tag="ACFB2Q_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC0918022.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLFSAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTTVYALANLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIP
                     LALSSLAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLLQSIFSTVWTPYIFR
                     AIEENATPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFTVVSCMLPP
                     LFYTLTEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGTRGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKNLHKLFHYLKKQGFPL"
     gene            4657..5898
                     /locus_tag="ACFB2Q_000976"
     CDS             4657..5898
                     /locus_tag="ACFB2Q_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFC0918023.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIR
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRTASLDELPELWNVLK
                     GEMSLVGPRPLLMQYLPLYNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITE
                     KAAALGFKLPVLIHPDATVSPSATIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDG
                     MTVAGNPAKPLTGKNPKTGTA"
     gene            6029..7204
                     /locus_tag="ACFB2Q_000977"
     CDS             6029..7204
                     /locus_tag="ACFB2Q_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC0918024.1"
                     /translation="MLNTALSPWPSFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            7252..9162
                     /locus_tag="ACFB2Q_000978"
     CDS             7252..9162
                     /locus_tag="ACFB2Q_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFC0918025.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFTAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFHVLTTAFAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSDHQKKTHDPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLKAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     TEGKTVMVTGAGGSIGSELCRQIIRRRPERLLLFELSEFALYTVEKELCEYCARNGIA
                     AEILPFLGSVQNRTLLTHIMTAFSVTTVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPANTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGRGGDVFVL
                     DMGESVKITDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADETMLPWHELSALLDRIRTACDRYDQQAIRDLLVNAPTGFTPTGGICDL
                     LWVCETHRKNAV"
     gene            9218..10510
                     /locus_tag="ACFB2Q_000979"
     CDS             9218..10510
                     /locus_tag="ACFB2Q_000979"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC0918026.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAIYCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(10562..11398)
                     /locus_tag="ACFB2Q_000980"
     CDS             complement(10562..11398)
                     /locus_tag="ACFB2Q_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC0918027.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKRQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(11693..11896)
                     /locus_tag="ACFB2Q_000981"
     CDS             complement(11693..11896)
                     /locus_tag="ACFB2Q_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC0918028.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(11874..12107)
                     /locus_tag="ACFB2Q_000982"
     CDS             complement(11874..12107)
                     /locus_tag="ACFB2Q_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC0918029.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNG
                     GTEIRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            complement(12119..12355)
                     /locus_tag="ACFB2Q_000983"
     CDS             complement(12119..12355)
                     /locus_tag="ACFB2Q_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918030.1"
                     /translation="MPIAGVSGSPRLRMCISVNADSSGNRICSLPLGRISLDGWRHTG
                     FQTAFLRNRQCGHIAGFAAILGAAAAGATITPSF"
     gene            12640..16074
                     /gene="dnaE"
                     /locus_tag="ACFB2Q_000984"
     CDS             12640..16074
                     /gene="dnaE"
                     /locus_tag="ACFB2Q_000984"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC0918031.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            16116..16370
                     /locus_tag="ACFB2Q_000985"
                     /pseudo
     CDS             16116..16370
                     /locus_tag="ACFB2Q_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16726..17656)
                     /locus_tag="ACFB2Q_000986"
                     /pseudo
     CDS             complement(16726..17656)
                     /locus_tag="ACFB2Q_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(17726..18269)
                     /locus_tag="ACFB2Q_000987"
                     /pseudo
     CDS             complement(17726..18269)
                     /locus_tag="ACFB2Q_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(18215..18430)
                     /locus_tag="ACFB2Q_000988"
     CDS             complement(18215..18430)
                     /locus_tag="ACFB2Q_000988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918032.1"
                     /translation="MSVFRINMTAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTASIPASPPAA"
     gene            18590..19249
                     /locus_tag="ACFB2Q_000989"
     CDS             18590..19249
                     /locus_tag="ACFB2Q_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC0918033.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDAPPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
BASE COUNT         4300 a         5926 c         5082 g         3986 t
ORIGIN      
        1 acagcaaccc tgtcgccgtc attaccgcga aagcgggaat ccagtccgtt cagtttcggt
       61 catttccgat aaattcctgt tgcttttcat ttctagattc ccactttcgt gggaatgacg
      121 gcggaagggt tttggttttt tccgataaat tcttgaggca ttgaaattcc agattcccgc
      181 ctgcgcggga atgacggctc aaaagttacg gaacgaaaaa caaccgaaac cggacaagtc
      241 ggattcccgc ctgcgcggga atgacggaat cttaagtttc cgtctttgtt ttctgttttc
      301 gcgggaataa tggggaacaa gccgtatttc agacggcatt ttcagttcgg ggtataatcc
      361 gaatacttgc gaccatctga atcatttgga caaactatgt gtcaactgct gggcatgaac
      421 tgcaatacgc cgaccgatat tatgttttcc tttgaaggct tccgccgcag gggcggcatt
      481 accgaccatc acgccgacgg tttcggcatc ggctttttcg aaggcaaagg cgtgcgcctg
      541 ttccatgacg acaagccgag tgcaaattcc cccgtcgccg acctcgtgcg cgcctaccag
      601 attaaatcgg aaaacgtcgt cgcccacatc cgcaaagcct cgcaaggtca aacctcgctg
      661 gcgaacaccc atccctttat gcgcgaaatg tggggcggct actggctgtt tgcccacaac
      721 gggcatttgg tcgatttttt ccccgaacag ggagaatttt tccaccccgt cggcacaacc
      781 gattccgaac gcgcgttctg ccacatcctc aaccgcctgc gcacccgctt tgccgcccgt
      841 cccgacgacg acacgctgtt tgacgcgatt gcggggctga cgcacgaaat ccgcaagttc
      901 gggctgttta acttcatgct ttcagacggc atttccctgt ttgcccacgc cagcacgctg
      961 ctgcactaca tcgtccgcca agccccgttc ggcaaagcgc gcctgctcga cgacgatgta
     1021 atggtcgatt ttgccgaagt aaccacgccg gacgaccgcg tcgccgttat ctccaccctg
     1081 ccgctgactc gcgatgaatc gtggtcccaa cttgccgtga acgaactggt catgttccgc
     1141 gaaggcaaca tcgtccggca cgaccgtccc gaaaatcccg tctatatgag tgccgaagaa
     1201 ggcctggaaa tcgcccgcgc cgccggcgta tcggtttgac gttcagacgg catcaggaag
     1261 aaacaccatg aaatgcccgt tttgcagcca cccagacacc cgagttgccg attcccgcct
     1321 gatggaagag cgcaataccg tccgccgccg ccgccactgc cccggctgcg gcaaacgctt
     1381 cggcacgctc gaaaccgtcg aactcaaaat gcccgccgtc atcggtccgg acaagaaacg
     1441 ttcgcccttc aatgcacaac gcctccgcaa cgacctgacc gccgccgccc gaaaatccgc
     1501 cctgacaccc gaacagattg acgaaaccgt ccgcctgacg gaacacaggc tctacacttc
     1561 gggtcagcgc gacatatcct catccgaact tgtcgaaatc gtgctggagg agctgttcgg
     1621 gcaaaacacg gaagccgccg tccgtttcgc cgccctgcac aaacgcttcg acagcccgga
     1681 acatttcgcc tcgtggctgg cgcaagccgt caaaacaggc ggcaaaacct gattccccca
     1741 acccatactg atacggtatc cctatgtttt cggacacaaa tatatccatg atggaaaacg
     1801 ccctccgact tgccgctttg gggcgttttt ccacttcacc caacccgcgc gtcggctgcg
     1861 ttatcgcaca cggcagacaa attgtcggac agggcttcca cgtcaaagcg ggcgaacccc
     1921 atgccgaagt ccacgccctg cgtcaggcgg gggaaatggc aaaaggcgcg accgcctttg
     1981 ttaccctcga accgtgcagc cattacggac gcacaccgcc ctgcgccgaa gccctgctcc
     2041 gttccggcgt aacacgcgtc gtcgccgcca tgcgcgaccc caacccgccg gttgcaggca
     2101 aagggcttgt cctgctcaaa gcagcgggca tcaagacgga atgcggacta cttgaaaaca
     2161 aagcacgcga actcaaccgg ggatttttat cgcgcatcga acgccgccgc ccctttgtcc
     2221 gcctcaaatg cgccgtttcg ctggacggca aaaccgccct ttcagacggc agcagctttt
     2281 ggattaccgg cgaggaagcg cgtgccgacg tacaggtttt gcgtgccgaa agctgcgcgg
     2341 tgctgaccgg catcggcacg gtgttggcag acaatccccg gctcaacgtc cgcgcttttc
     2401 caactttgcg ccaacccgca cgcatcgttt tagacagccg cctgcgcctg cccccgaaca
     2461 gccatttggt taccgacgga caatctccga cctacatcgc caccttggaa cgcgatgaag
     2521 acaaactccg cccctatcgg gaacacgcac acatccgcat cctgatgccg tctgaaacgg
     2581 cagacggcaa aatcgacctg caccatctga tgcgcctcct tgccgacgaa ggtttcggcg
     2641 aaatcatggt cgaagcaggc tccgaactca catccgcatt tttggcggaa aatttggcgg
     2701 acgaaatcgt gctgtaccgt tcgcccaaaa tcctcggcgg cggcaaagac ctgttttccc
     2761 tgcccgaaaa ccgtgccgcc ctttccgcac cgcccttgtg gacacccgtt tcaagcgaaa
     2821 tcctcggaca cgacatcaaa accgtgttcc ggaaaaacgg caacgccttt taaagggttt
     2881 gccgccgttt tgctatataa taacgccgat aaaaaacggc ccgttcagac cgccgcccgc
     2941 ccggaaaagc gcaacccgga ctgccgcacc ccgccggcag ccgcgacggc ctgaaagccg
     3001 tcaaattccg atcaagaaag gcttcagacg gcacaggcag catcacgccg ccgcccggac
     3061 atcaaaaatg gacacaaaag aaatcctcgg ctacgcggca ggctcgatcg gcagcgcggt
     3121 tttagccgtc atcatcctgc cgctgctgtc gtggtatttc cccgccgacg acatcgggcg
     3181 catcgtgctg atgcagacgg cggcgggact gacggtgtcg gtattgtgcc tcgggctgga
     3241 tcaggcatac gtccgcgaat actatgccgc cgccgacaaa gacactttgt tcaaaaccct
     3301 gttcctgccg ccgctgctgt tttccgccgc gatagccgcc ctgctgcttt cccgcccgtc
     3361 cctgccgtct gaaatcctgt tttcgctcga cgatgccgcc gccggcatcg ggctggtgct
     3421 gtttgaactg agcttcctgc ccatccgctt tctcttactg gttttgcgta tggaagggcg
     3481 cgcccttgcc ttttcgtccg cgcaactcgt gcccaaactc gccattctgc tgctgttgcc
     3541 gctgacggtc gggctgctgc actttccggc gaacaccgcc gtcctgacca ccgtttacgc
     3601 gctggcaaac cttgccgccg ccgccttttt gctgtttcaa aaccgatgcc gtctgaaggc
     3661 cgtccggcgc gcgccgtttt cgcccgccgt cctgcaccgg gggctgcgct acggcatacc
     3721 gctcgcactg agcagccttg cctattgggg gctggcatcc gccgaccgtt tgttcctgaa
     3781 aaaatatgcg ggcctggaac agctcggcgt ttattcgatg ggtatttcgt tcggcggggc
     3841 ggcattattg ctccaaagca tcttttcaac ggtctggaca ccgtatattt tccgtgcaat
     3901 cgaagaaaac gccacgcccg cccgcctctc ggcaacggca gaatccgccg ccgccctgct
     3961 tgcctccgcc ctctgcctga ccggaatttt ctcgcccctc gcctccctcc tactgccgga
     4021 aaactacgcc gccgtccggt ttaccgtcgt atcgtgcatg ttgccgccgc tgttttacac
     4081 gctgaccgaa atcagcggca tcggtttgaa cgtcgtccgc aaaacgcgtc cgatcgcgct
     4141 tgccaccttg ggcgcgctgg cggcaaacct gctgctgctg gggcttgccg taccgtccgg
     4201 cggcacgcgc ggcgcggcgg ttgcctgtgc cgcctcattc tggttgtttt ttgttttcaa
     4261 gacagaaagc tcctgccgcc tgtggcagcc gctcaaacgc ctgccgcttt atatgcacac
     4321 attgttctgc ctggcctcct cggcggccta cacctgcttc ggcacaccgg caaactaccc
     4381 cctgtttgcc ggcgtatggg cggcatatct ggcaggctgc atcctgcgcc accggaaaaa
     4441 tttgcacaaa ctgtttcatt atttgaaaaa acaaggtttc ccattatgaa aatcatcctg
     4501 actacttcta tgtcggttct gggaggcacg ggcacgacat catcgattgc caaatgtcgt
     4561 gcaaaaccga ccccgttcag tccgacgaaa tcgtccgccg cttcaggcgc gacatttcct
     4621 atcggaaaat cgtcaacctg attgaaagat tggcaaatga gtaaagccgt caaacgcctg
     4681 ttcgacatca tcgcatccgc atcggggctg attgtcctgt cgcccgtgtt tctggttttg
     4741 atatacctca tccgcaagaa tctgggttcg cccgtcttct tcattcggga acgccccgga
     4801 aaggacggaa aaccttttaa aatggtcaaa ttccgttcca tgcgcgacgc gcttgattca
     4861 gacggcattc cgctgcccga cagcgagcgg ctcacgccct tcggcaaaaa actgcgcacc
     4921 gccagtttgg acgaactgcc tgaactgtgg aacgtcctca aaggcgagat gagcctggtc
     4981 ggcccccgcc cgcttttgat gcagtatctg ccgctttaca acaaatttca aaaccgccgc
     5041 cacgaaatga aaccgggcat taccggctgg gcgcaggtca acgggcgcaa cgcgctttcg
     5101 tgggacgaaa agttctcctg cgatgtttgg tacaccgaca atttcagctt ttggctggat
     5161 atgaaaatcc tgtttctgac agtcaaaaaa gtcttgatta aagaaggcat ttcggcgcaa
     5221 ggggaagcca ccatgccccc tttcgcgggg aatcgcaaac tcgccgttat cggcgcgggc
     5281 ggacacggca aagtcgttgc cgagcttgcc gccgcactcg gcacatacgg cgaaatcgtt
     5341 tttctggacg accgcaccca aggcagcgtc aacggcttcc ccgtcatcgg cacgacgctg
     5401 ctgcttgaaa acagtttatc gcccgaacaa ttcgacatca ccgtcgccgt cggcaacaac
     5461 cgcatccgcc gccaaatcac cgaaaaagcc gccgcgctcg gcttcaaact gcccgttctg
     5521 attcatcccg acgcgaccgt ctcgccttct gcaacaatcg gacaaggcag cgtcgtaatg
     5581 gcgaaagccg tcgtacaggc cggcagcgta ttgaaagacg gcgtgattgt gaacactgcc
     5641 gccaccgtcg atcacgactg cctgcttgac gctttcgtcc acatcagccc gggcgcgcac
     5701 ctgtcgggca acacgcgtat cggcgaagaa agccggatag gcacgggcgc gtgcagccgc
     5761 cagcagacaa ccgtcggcag cggggttacc gccggtgcag gggcggttat cgtatgcgac
     5821 atcccggacg gcatgaccgt cgcgggcaac ccggcaaagc cccttacggg caaaaacccc
     5881 aagaccggga cggcataagc gattaaaata aacttccgta caaaccgatt ttgacaacac
     5941 ctgcggcgcg cgcccgattc ttcggaacac gcccccttca gacggcatag ggtcggaaat
     6001 gccgtccgaa cccgacggac caaacaccat gctgaacacc gccctttccc catggccgag
     6061 cttcacccaa gaagaagccg atgccgtttc caaagtcctg ctgtccaaca aagtcaacta
     6121 ctggacgggc agcgaatgcc gcgaatttga aaaagaattt gccgcctttg ccggcacgcg
     6181 gtacgccgtc gccctttcca acggcacgct tgcgctcgat gccgcactca aagcaatcgg
     6241 cataggcgcg ggcgacgatg tgattgttac ctcgcgcacc ttcctcgcct ccgcctcctg
     6301 cattgtgaac gcgggcgcaa accccgtgtt tgccgacgtg gatttgaaca gccaaaacat
     6361 cagcgcggaa accgtcaaag ccgtgctgac cccgaatacc aaagccgtca ttgtcgtcca
     6421 ccttgccggt atgcctgccg aaatggacgg catcatggct ttggcaaaag aacatgattt
     6481 gtgggtgatt gaagactgcg cccaagcgca cggcgcgacc tacaaaggca aatccgtcgg
     6541 ctctatcggg cacgtcggcg cgtggtcgtt ctgtcaagac aaaatcatca ccaccggcgg
     6601 cgagggcggt atggttacga ccaacgacaa aaccctgtgg gaaaaaatgt gggcgtacaa
     6661 agaccacggc aaaagctacg atgccgtgta ccaccgcgaa cacgcgcccg gtttccgctg
     6721 gctgcacgaa agtttcggca ccaactggcg catgatggaa atgcaggctg tcatcggccg
     6781 catccagctc aaacacctgc ccgaatggac ggcgcgccgc caagaaaacg ccgccaagct
     6841 ggcagaaagt ttgcgcaaat tcaaaagcat ccgcctgatt gaagtcgccg gctacatcgg
     6901 acacgcgcaa tacaagttct acgccttcgt caaacccgaa cacctgaaag acgactggac
     6961 gcgcgaccgc atcgtcagcg aactgaacgc gcgcaacgtc ccctgctatc aaggcggctg
     7021 ctccgaagtg tatttggaaa aagccttcga caacacgccg tggcggccca aagagcggct
     7081 gaaaaacgcc gtcgaactgg gcggcaccgc cctgacgttc ttggtgcacc cgacgctgac
     7141 cgacgacgaa atcgcgttct gcaaaaaaca catcgaagcc gtcctgaccg aagccgcacg
     7201 ataacccttc agacggcata tgccgcctga aagcacatac cgccccacga tatgaatctg
     7261 gaaaccctga tcgccctgcc gcgcaacatc aagaaaatct gtttcctcat acacgatttt
     7321 ctgatgattt tcaccgcctt ttggttcacc caaagcctga aggccgacta ctcggacgaa
     7381 tggttcgatt ttgccaactg gcagtctttt ttgctgactg ccttgctgac catcacatta
     7441 tttatccgaa tggggcttta ccgcgccgtt acacgctttg tcagtttcca cgtcctgacc
     7501 accgcctttg ccggcagcct cgcctccgcc gtattgttct tcctcaacac attgattttt
     7561 gaagaaaggc tgcgcctcgc cctgccgatt gtctatttct tactgctgtt tgtttccgtg
     7621 accggctcgc gtatggtttt gcgcggactc ctgtccgacc accagaaaaa aacacatgac
     7681 cccgtcatca tttacggcgc ggggcgttcg ggtcggcagc tccttgaagc cgtcaaacag
     7741 atgcgcgaat attccgccgc cgcctttgtg gacgacaacc ccaagctttg gcacaccgtc
     7801 atctacgacc ttgccgttta ccagcccgat gccatcgcct tcctcatcga acgctacggc
     7861 gtggaaaaaa tcctgctcgc cattcccagc gcgactcagg aacaacgccg ccgaatcatc
     7921 agcaaactga aagcctatcc gtgcgaagtg ttgaccattc ccggaatgaa agacctgatg
     7981 gacgggaaaa tcagcatcgg cacgctcaaa aaaatctctg tgtccgacct gctcgggcgc
     8041 gattccgtcg cacccgacga ccgcctcatg aatgccgaca ccgaaggcaa aaccgtcatg
     8101 gtaaccggcg cgggcggctc catcggttcg gaactctgcc gccagattat ccgccgccgc
     8161 cccgaaaggc tgctgctgtt cgagctgtcc gaattcgccc tgtacaccgt tgaaaaagaa
     8221 ttgtgcgaat actgcgcccg aaacggcatc gccgccgaaa tccttccctt tttgggttcg
     8281 gtgcaaaacc gcacgctgct cacgcacatt atgaccgcct tttccgttac gaccgtctat
     8341 cacgccgccg cctacaaaca cgtccctatg gtcgagttca acaccgtcga gggcatacgc
     8401 aacaacatct tcggcacact cgaatgcgcg cttgccgcca cggcttcggg cgtggaaacc
     8461 ttcgtcctca tctccaccga caaagccgtc cgccccgcca acacgatggg tgcgggcaaa
     8521 cgcatggcgg aactctgcct tcaggcactc gccgccgaac ccggacaaaa aacccgcttc
     8581 agcatggtac gtttcggcaa tgttttaggc tcgtccggct ccgtcgtccc gctgtttgaa
     8641 aaacagattg ccgaaggcgg ccccatcacc ctgacccacc ccgacatcac gcgctatttc
     8701 atgaccatac ccgaagcggc gcaactggtt atccaagccg gcgcgatggg cagaggcggc
     8761 gacgtgttcg tcctcgatat gggcgaatcc gtcaaaatca ccgaccttgc ccgccaaatg
     8821 attaccttaa gcggactcaa acccaaaacg cccgaacagc ccgacggcga catcgaaatc
     8881 ctcattaccg ggctgcgccc cggcgaaaaa ctctacgaag agttgctcat cggcgacaac
     8941 gtccgcaaaa ccggccatcc gcgcatcatg accgccgacg agaccatgct gccgtggcac
     9001 gaactctccg ccctgctcga ccgcatccgc accgcctgcg accgttacga ccagcaggca
     9061 atccgcgacc tgctcgtcaa cgccccgacc ggctttaccc cgaccggcgg catctgcgac
     9121 ctgctttggg tatgcgaaac acacagaaaa aatgccgtct gaaccttcag acggcatacc
     9181 gtacaaacca atctgcctta cacacactga gtttgacatg cagttctcgg cattcggcga
     9241 aaaattcacg caacacagcg gcatcctgca actgatggac gacctcggcg acgcgctcaa
     9301 aagcgacaag cccgtcaata tgctcggcgg cggcaacccg gcgcgcattc ccgaaatcga
     9361 tcgggcgttc gccgacatat tttccaagct ggcggcggaa cacgccgtcg agaacatcgg
     9421 caactactcc aatccccaag gcgatgccgc gctgattgac gcgctgaccg ccttccccaa
     9481 ccgcgaatac ggctggaacc tgaccgtcgg caatatcgcg ctgaccaacg gttcgcaaaa
     9541 cgcgtttttc tatttattca acctcttcgg cggcaaattc aagctttcag acggcacatc
     9601 cgcagaaaaa gccattttgt tgccgctcgc gcccgaatac atcggctatg ccgacgtgca
     9661 tgtcgaaggg cggcacttcg tttccgtcaa acccaaaatc gaaaacgtcg aacacgaagg
     9721 cgaagcaggc ttcttcaaat accgcgtgga ctttgacgca ctggaaaacc tgcccgaact
     9781 caaagcgggc aaaatcggcg cgatttactg ttcgcgcccg accaacccga ccggcaacgt
     9841 gttgaccgac ggcgaaatgg cgcgtttgga cgctttggcg cgtgaacacg gcattccgcc
     9901 gattatcgac aacgcctacg gaatgccgtt ccccaacatt attgacagcg gcgtaacgct
     9961 gaattggcac gaaaacatca tcctctgctt cagcctttcc aaagtcggcc tgccgggcgt
    10021 tcgcaccggc atcatcgtcg ccgcgcccga agccgtcaaa gccgtcagca gcctgaacgc
    10081 gattgtgaac cttgccccca cgcgcttcgg cgcggccatc gccgccccgc tgctggaaag
    10141 cggcgagatg aaacggcttg ccgaccaagt catccggccg ttttaccgca atcaggcgca
    10201 aaccgccgtt tcgctgctca agcgcgagct gggcgcgtac ccgatgaaaa tccacaaacc
    10261 cgaaggcgcg attttcctgt ggctctggtt tgaaaacctg cccgtttcgt cgcaaaccct
    10321 gtacgaaatg ctcaaagccg aaggtacgct gattattccg ggcgaacatt tcttcgtcgg
    10381 catcgacacg caagactacc cgcacgcggg cgagtgcatc cgtatgagta tcgcacagga
    10441 cgctcaaacg ctggaaaaag gcatcgccgc catcggcaaa accgtccgaa aaccgtacga
    10501 caacgtttaa aacgcaaaaa atgccgtctg aagacctttc agacggcatt ttcatccgca
    10561 ttcaatgtcg ggaaaaatgc gcccgcaccg gtttgcagtt ttccggaaga tcgggacacg
    10621 cgccgaggat gccgccgctg aagtcgtcta aacggtggaa gtcgctgccc gcgcttgcca
    10681 acaatccgaa gcggtctgcc aaaagcgcgt agttgaggcg gtcgtttttg cagcagttgc
    10741 cgctgtggac ttcgatgccc gcgccgccga ggtttttaaa ttcttcaaac aggttgcgct
    10801 tggcggttgc cgacaaatca taacgcatcg gatgggcaat caccgccatg ccgcccgcgc
    10861 cgttgacggc ggagacgcag tctgccagcg tcgcccattc gtggcgtacc gcgcaggatt
    10921 tgccgtcgcc caagtatttg gtgaacgcct gccgcttgtt tttgacgtgt ccgtttttga
    10981 tgaggaactc ggcgacgtgg gtgcggctga ccatttcttt gtttgccgcc agcgccagcg
    11041 cgccgtcgta tgcgccgccg atgcctttct tttcgagctt ggcggcgatg gcttcaagac
    11101 gtttcagacg gcctttgcgg acatctgcca acaggttttg caggttttcg tcctgctcgt
    11161 cgaaatccaa accgacaacg tgtatggtac gcccgcgcca cgttaccgaa atttccacgc
    11221 cgttaatcag gcgcaaaccg agcctgtcgg cttcggcacg cgcttcggag atgccgcccg
    11281 tgtggtcgtg gtcggtcaac gccagcagcg tgcagccgtt ttgatgtgcg aggcggacga
    11341 cttcggcggg ggggagcata ccgtcggaaa cggtggaatg gcagtgcagg tctatcatgg
    11401 ggtgttatgt ggtggtgaat gaaggacggg atgtttcaat gttgtcggac agtgtggcgg
    11461 cggcggtcat tttaaaaaac aggtgtatgg caaatgcaaa ggaaaagccg ctatgccgtc
    11521 attcccgcgc aggcgagaat ccagactttg atttattgga aatgtttaag gttaaccgct
    11581 atttcaaact tccggattcc tgctttcgag ggaatggcga tatgaacgtt ttcaatttta
    11641 atctactata aaagaccgtc tgaaaacgtg gttttatcaa gcccaaccgt tttcagacgg
    11701 catcgtccgg ccaacccgct tccttcagtt ttttccgttg cgcatcgtag cgtgccttgt
    11761 ccgcccagta aatcgtctga atgcacgcct cgccgcagtc gctgccgcag cattcccacg
    11821 actcggggcg gacgggttcg tccaaaagcg gctcgcccaa aagggcttcg gctttatgct
    11881 tcagggtcgt atccatcggc gatttccaag cgcgcgccgt caaactcgat gacttctccg
    11941 ccgcgtattt tggccgtttt gcggatttcg gttccgccgt tgcgcaacac cagcccttcg
    12001 gcgataaatg cttttgcctg tccgccgctt tcggcaagtc cggccaattt caagaggtcg
    12061 cacaaggcga tgtattcgtt gtcttcgaga tagacggtgg cttccataat gtcccctttt
    12121 agaaagaggg cgttattgta gcacctgccg ccgccgcacc caaaattgcc gcaaaaccgg
    12181 cgatgtgtcc gcactgcctg ttccgtaaaa atgccgtctg aaaccccgta tgccgccatc
    12241 cgtccaaaga aatcctgccc aacggcaggc tgcaaatcct gttccccgac gaatccgcat
    12301 tgacgctgat gcacatcctc aaacgcggac tgcccgatac accggcaatc ggcatcaaaa
    12361 cgaaatcaaa aacctgtttg aacgtaaaat ctatttaact gcttgccgtc cgtccgaata
    12421 aggtatatag ttccttataa ctattttgat agtcctttat atctatcaat actccttggg
    12481 aagcctccgc cacacggcag gaggcatttt tttgccgtcg taaaagctca aaaaacattt
    12541 gcaggtcatg ccgtctgaat ccgaaacggc attacctgca ccgccatcta aagacaaccc
    12601 tgctacaata cgccttttat tgtccacgcc gattttgcca tgaccgagcc gacctacatc
    12661 cccctgcgcc tgcataccga attctccatt accgacggta tggtgcggat taaaaaactg
    12721 attgccaaag cgcaagaata cggtttgcct gctttgggca tcagcgattt gatgaacgaa
    12781 ttcggtttgg tgaaattcta taaagcctgc cgcggcgcag ggattaagcc cgtcggcgcg
    12841 gcggacgtgt ggataggcaa tccgaatgcg cccgacaagc cgttccgcgc catgctggtt
    12901 atccgcaacg atgcgggtta tctgcgcttg agcgagcttt tgacggaggc ttatgtcggc
    12961 caagaccgca atatccatca tgcggaactc aatcccgaat ggctggaaaa cggcgacaac
    13021 agcggcttga tttgtttgag cggcgcgcat tacggcgaag tgggcgtgaa tctgttgaac
    13081 ggcaatgaag acgcggcgcg tgcggcggcg ttgaaatatg cggcatggtt tcccgatacg
    13141 ttttatttag agctgcaacg cctgcccgaa cgccccgaat gggaggcttg cgtttccggc
    13201 agcgtgaagc tggcggagga actgggtttg ccggtggtag caacgcatcc gacacagttt
    13261 atgaaccgcg acgatttcaa cgcgcacgag gcgcgggtgt gcatcgcggg cggctgggtg
    13321 ctgacggaca agaaacgtcc gcgcgatttc acgccgagcc agtttttcat tccgccggaa
    13381 acgatgctgg agcgtttcgc cgacttgccc gaagccttgg aaaacacggt ggaaatcgcc
    13441 aaacgctgca acttgcacat cacattgggc aaaaacttcc tacccctttt ccccacgccc
    13501 gacggtttat cactcgatga ctgtctcatc aaactctcca acgagggttt gcaggaacgc
    13561 atggttcagc tttatcccga cgaggcggaa cgggcggcaa aaatgccgga atatcaggag
    13621 cggctggact ttgagctgaa catcatcatc caaatgaaat tccccggcta tttccttatc
    13681 gtacaagact ttatcaactg ggcgaaaacg cacggctgtc cggtcgggcc gggacgcggt
    13741 tcgggcgcgg gttcgctggt ggcgtattcg ctgaagatta ccgacctcga cccgctgaaa
    13801 tacgcgctgc tgttcgagcg tttcctaaac cccgaacgcg tttccatgcc cgactttgac
    13861 gtggacttct gtcaggcaaa ccgcggccgc gtgattgaat acgtgcgcga aaaatacggc
    13921 gctcaggcgg tcagccagat tgtgactttc ggcacgatgt cgtccaaagc ggtcatccgc
    13981 gacgtggggc gtgtgctgga actgccgttt atgctgtgcg acaaactgtc caagctgatt
    14041 ccgttggaag ccaacaaacc cctgagtttg gacgacgcga tgaaggcgca gccgcagatt
    14101 caggaattgc ttgaagcgga agaagcggac gaactgatta cgctggcgaa aaaactggaa
    14161 gacctgacgc gcggtttggg tatgcacgca ggcggcgtgt tgattgcgcc gggcaagatt
    14221 tccgattaca gccccgtgta tcaggcggac gaatccgcct cgcccgtatc catgtacgac
    14281 aagggcgacg tggaagacgt gggtttggtg aagttcgact ttttgggcct gcgcaacctg
    14341 accattatcg aaatggcgca gaacaacatc aaaaacaccg ccggcgatat cgtcgatgtc
    14401 ggcaagattc cgcttgacga ccagactgcc taccaaatct tccgcgatgc gaacaccacc
    14461 gccgtcttcc agttcgagtc gaccggcatg aaaaaaatgc tgaaaacggc gcatacgacc
    14521 aagtttgaag aactcatcgc cttcgtatcg ctctaccgac ccggcccgat ggacaacatt
    14581 cccgacttcg tcgcacgcat gaagggacag gaattccaat acatccaccc gctgctggaa
    14641 ggcatcctcg cgccgaccta cggcattatg gtgtatcagg aacaagtgat gcaggcggcg
    14701 cagattatcg gcggctactc gctcggcggc gcggacctgc tgcgccgcgc catgggtaag
    14761 aaaaagcccg aagagatggt gaaacaccgc gaaatcttcg ccgaaggtgc ggcaaaacaa
    14821 ggcatttcgc gcgaaaaatc cgacgaaatc ttcaactaca tggaaaaatt cgccggctac
    14881 ggtttcaaca aatcccacgc cgccgcctac gccctgattt cctaccagac cgcatggctc
    14941 aaagcccact atcccgccga atttatggcg gcgacgatgt cgtccgaatt ggacaacacc
    15001 gaccagctca aacatttcta cgacgactgc cgcgccaacg gcatcgagtt cctgccgccc
    15061 gacattaacg aatccgacta ccgcttcacg ccgtatccga acatgaaaat ccgctacgca
    15121 ctcggcgcga ttaaaggcac gggcgaagcc gccgtcgaat ccatcatcgc cgcgcggcaa
    15181 agcggcggca aatttaccgg cctgttggac ttctgcgagc gcgtcggcaa agaacacatg
    15241 aaccgccgca ccctcgaagc cctgatacgc ggcggcgcgt tcgacagcat cgaacccaac
    15301 cgcgccatgc tcttggcgaa catcgacctt gccatggaca acgccgacca aaaagccgcc
    15361 aacgccaatc agggcgggct gtttgacatg atggaagacg ccatcgaacc ggtgcggctc
    15421 atcgacgcac ccatgtggag cgaatcggaa aaactcgccg aagaaaaaac cgtcatcggc
    15481 ttttacctgt ccggccaccc gttcggcccg tatgcccaag aagtccgcca aatcgccccg
    15541 accaaattag gccgtctgaa accgcaagac agcgtgcgcc tcgccggatt cgttaccgcc
    15601 gtgcgcacca tgatgggcaa acgcggcaaa atcgccttcg tcagcctcga agattcgagc
    15661 gggcaggctg aaatcatggt cggcggacaa acgctggaaa actgcgccga ctgcctcaaa
    15721 gccgaccaag tgctgattat cgaatccaaa gtcagccgcg acgattacgg cggcggcgac
    15781 gggctgcgca tcatggcaaa ccaagtcatg accctgcaaa cggcgcgcga acgctacgcc
    15841 cgcagcctca gcctcgccct tgccccgcat cacgacatcg gcggactggt acggctgctc
    15901 gccgcccacc aactgcccga cacgccgcgc atcccgcttc aattgtcgta tgccaacgaa
    15961 aaagcgtcgg gcaggcttca agtgcctccg aaatggacgg ttacaccgag ttccgcattg
    16021 ttcggcgaac tggaaacatt gctcggcagc cggtcggtgc gcgtgaattg gtaaccaaaa
    16081 atacaaatgc cgtctgaagc ccaaaaccgg tttcattcgt actttattcg aatgattgaa
    16141 taaaagtaac tgccaagaaa aacgtatttt ttggttattt cgccagccta aataaatcaa
    16201 ccggggcgat tgatatccgt gtacatgaca cagacagcac caaagggaaa aacggcattt
    16261 tcaaaagtat cggtgtcaaa accgcccttt cattccaaaa ataccaaatc gacgaaccgg
    16321 gcaaagaaat cagaccgtgc cgcctgaaaa aacgcccgcc cgtccgttaa attaatctat
    16381 ccctgtttca ggcggcctga agcagggatt tttatatcaa gataaaatga gaacgggagc
    16441 gacaaccacg ggcaaagtcg gcgcgaacta tcaaaaattg tagcactgcg aaatgaggac
    16501 gggagcaata acccttaggt agctcttgtt atagcggatt aacgaaaatc aggacaaggc
    16561 gacgaagccg cagacagtac aaatagtacg gaaccgattc acttggtgct tgggtgcctt
    16621 agggaatcgt tctctttgag ctaaggcggg gcaacgccgt accggttttt gttaatccgc
    16681 tatattttcc gatgcaaaac aaagcagtca tatatttgat tccccctacc gttgccacgc
    16741 ttccctcaaa tattcgacgc agtcggtcag cgagtaaaac gggacattgc cgtggtcggc
    16801 attcggatat tcccggaaaa atacggcggt cccgcgcctg tccaactctg ccgccatttg
    16861 ttcggcctgc cctgccatgt cgcgttcttc cctgcgtttg caaccgctgc tccgctcgag
    16921 tgcgccgatg ttgaggcagg catcgatgcc gtccagccgg ttttcagacg gcataaagtc
    16981 gagtatccgc ctgttgtgcc accaaatcga ggggatacga gccaatgccg tctgaaacag
    17041 cggtgggaaa gcagggaata cagtccgaac agtgcgccga acgagtgtcc gaatacggcg
    17101 gtttcattac ggttaagggt gtagcggctt tctaaaaagg cggtcagttc gctgtcgata
    17161 aagtcggcga agcggtctgc ctgtccgaac tgccgccgtt cgtctgctgt ggcgttgtct
    17221 ccaagcggcg gcgtgtagtc ggcggcacgc tgcgccaggt cgcgcacact gcctgtcgtg
    17281 tagccgatac cgacaatcag gcagggggcg ttgcttcggg taacggggtt gttcatcagc
    17341 gactgcatga tgttgtacag cgcgggaaaa aggcttcgcc gtcgaggaca aagaggacgg
    17401 gatagccttc agactgcatt tcgccgagtg ttgccgtctg aatccgatag attcgccccg
    17461 tgcaggtgga tttgatttcg gtttcaaagg cttggggcag tatggcaggt tggaatgtgt
    17521 cggtcggtat gggtttcatg atgttcggct tgtgggtcag actggcggca agcggtaaaa
    17581 ccgaaccggc aaagcggtct gcctgccggt tcggtctgtt ttccttcgca atgccatact
    17641 ccggttgtga gcgcataggg tatgccgcgc agcttgttgt ggtttgatta tgctgcggct
    17701 gccgtttcag acggcatatt ggtctttaaa aactgtaacg caggtttgcc gtcaggctgc
    17761 gctccgaacc gggaatgtta aaggtgttcg tgccgccgac gcgggcgtag taatggcggt
    17821 tgaagatgtt gtcggcattg atttgcagct tcagcttggg cgtgaagcgg tatgccgcca
    17881 tcgcatcgaa cgtggcataa ccgcctgcat gcatccctgc agatgaagta atgccgctca
    17941 tcgcgttcac gccgccgccg acggtcagcc cgggcgtaac ttggtaagtc gtccacaggt
    18001 ttgcgctgtg tttgggcacc agcaggaaga tgccgtcgtc gcgtggattg gcggcggttt
    18061 tgatttggct gtgcagatag ctgtaacctg catggatttg ccatttcggt gtaatcgcac
    18121 cgctgatttc ggtctcaacg ccttccatca cgcgtttgcc caatgcggcg taacgggtgt
    18181 tgcggttgtt cgggttcggc ggcgtttttg tctttcaggc ggcaggggga gacgcgggta
    18241 ttgaggcggt catccatata gctgccttta acgagctgta atcgccgttt ttctcggtgg
    18301 caatcgcgtt gtacgaacgc tgccctttga tatggacggt ttccaaatcc gccgtttgtg
    18361 cggcaaaaac cggagacgag agtgctgcca aaaccgtggc ggcggtcata ttgatgcgga
    18421 aaactgacat aaactgtccc atctgtataa atgataatgg ttctatttta ataaagcaca
    18481 acgcggcttg ttcggaaaaa catatcgctc agccgacaaa ttttgtcgaa aatgcgacac
    18541 gtctgcgttt tccgcataaa atctgctttt ttactgcaac caacctgcca tgaccccgcc
    18601 caaactcatc atcttcgact gggacggcac gcttgccgat acgacccaac ccatcatcga
    18661 caccatgcgc cgcagcttcg ccgaatgcgg ttttccgccg cccgaagcgg agcgcgtccg
    18721 cagcctgatc ggatacagcc tgcccgaaat catccgcgcc ctgctcgaaa tgccttctga
    18781 agctgccgtt gccgacatcg cgcgcactta ttccgcgcat tacctcaatc ccaacaaccg
    18841 caatatgaca ctctttcccg atgccccgcc ctgtctggac aagctcaaag cgcaaggctt
    18901 ccggcttgcc gtcgccacgg gcaaagggcg ggcgggtttg gacaacgcca tcagccaaac
    18961 cgccaccggc ggctattggc tggccaccgc ctgcgcgggg gaatacccct ccaaaccctc
    19021 gcccgaaatg gtattcggac tctgcggcga actgggactc gacccgaaag aggcattggt
    19081 tgtcggcgat acggcgcacg acctgcatat ggcggcaaac gcaggcgcgg cggcagtcgg
    19141 cgttgccacc ggcgcacatt cgcgcgaaca gctccttggc gcaccgcatc tcgccgtatt
    19201 ggacggtttg tccgaactgc ccggttttct tgcacgacat tacgcctgat tggtttccgc
    19261 atccggcacg cggcaaaaat gccgtctgaa gcct
//
LOCUS       DAXDSD010000037        19148 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXDSD010000037 DAXDSD010000000
VERSION     DAXDSD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19148
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.037"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..137
                     /locus_tag="ACFB2Q_000990"
     CDS             <1..137
                     /locus_tag="ACFB2Q_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC0918034.1"
                     /translation="IKSSPWLPDSVGIHERYERFTTMLRYIFTEKDIVNVRFDYYNKK
                     "
     gene            complement(201..2414)
                     /locus_tag="ACFB2Q_000991"
     CDS             complement(201..2414)
                     /locus_tag="ACFB2Q_000991"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC0918035.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATSEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
     gene            2557..3198
                     /locus_tag="ACFB2Q_000992"
     CDS             2557..3198
                     /locus_tag="ACFB2Q_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC0918036.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            complement(3270..3908)
                     /locus_tag="ACFB2Q_000993"
     CDS             complement(3270..3908)
                     /locus_tag="ACFB2Q_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC0918037.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            4164..5015
                     /locus_tag="ACFB2Q_000994"
     CDS             4164..5015
                     /locus_tag="ACFB2Q_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC0918038.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            5081..5743
                     /gene="exbB"
                     /locus_tag="ACFB2Q_000995"
     CDS             5081..5743
                     /gene="exbB"
                     /locus_tag="ACFB2Q_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC0918039.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            5746..6180
                     /locus_tag="ACFB2Q_000996"
     CDS             5746..6180
                     /locus_tag="ACFB2Q_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC0918040.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQANKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(6681..7109)
                     /locus_tag="ACFB2Q_000997"
     CDS             complement(6681..7109)
                     /locus_tag="ACFB2Q_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC0918041.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(7174..8922)
                     /locus_tag="ACFB2Q_000998"
     CDS             complement(7174..8922)
                     /locus_tag="ACFB2Q_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC0918042.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALNGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
     gene            9248..10681
                     /gene="ccoN"
                     /locus_tag="ACFB2Q_000999"
     CDS             9248..10681
                     /gene="ccoN"
                     /locus_tag="ACFB2Q_000999"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC0918043.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            10708..11319
                     /gene="ccoO"
                     /locus_tag="ACFB2Q_001000"
     CDS             10708..11319
                     /gene="ccoO"
                     /locus_tag="ACFB2Q_001000"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC0918044.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            11324..11494
                     /locus_tag="ACFB2Q_001001"
     CDS             11324..11494
                     /locus_tag="ACFB2Q_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC0918045.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            11518..12837
                     /gene="ccoP"
                     /locus_tag="ACFB2Q_001002"
     CDS             11518..12837
                     /gene="ccoP"
                     /locus_tag="ACFB2Q_001002"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HFC0918046.1"
                     /translation="MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQ
                     AASEAKPAAAEPKAEEKAAPAAKADGKQVYETVCAACHGNAIPGIPHVGTKADWADRI
                     KKGKDTLHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            13181..14590
                     /locus_tag="ACFB2Q_001003"
     CDS             13181..14590
                     /locus_tag="ACFB2Q_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC0918047.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            14691..17900
                     /gene="recC"
                     /locus_tag="ACFB2Q_001004"
     CDS             14691..17900
                     /gene="recC"
                     /locus_tag="ACFB2Q_001004"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC0918048.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKCALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAADRMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLLETLFQAGPDDQKAVQNLE
                     NEWVKWQEESTLAGFFGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPRPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHAEQIAETYIGARREGQDFSQTAARIAAESLLPSG
                     ELGKLWQQSFQTAAKQIDTAVLNSPKLPPFPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVKSGETEVLAEIAQDRALQLLSEWI
                     VFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGTGEA"
     gene            complement(17970..>19148)
                     /gene="mtrF"
                     /locus_tag="ACFB2Q_001005"
     CDS             complement(17970..>19148)
                     /gene="mtrF"
                     /locus_tag="ACFB2Q_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC0918049.1"
                     /translation="TTFMVVFTGILSNTASELGYVVLIPLSAVIFHSLGRHPLAGLAA
                     AFAGVSGGYSANLFLGTLDPLLAGITQQAAQIIHPDYVVGPEANWFFMAASTFVIALI
                     GYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWAGVVFIALSALLAWS
                     IVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRITRSLRGEREVVNAMA
                     ESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVGLAGSVLFIGFILIC
                     AFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDSVTNIITPMMSYFGL
                     IMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLGLPVGPGAPTFYPVP
                     "
BASE COUNT         4542 a         4654 c         5365 g         4587 t
ORIGIN      
        1 caattaaatc ttccccttgg ttgcctgata gtgtgggaat tcatgaacgt tatgaaagat
       61 tcacaacgat gctccgttat atttttaccg agaaagacat agtcaacgtg cgatttgatt
      121 attacaacaa aaaatagcaa aacccggcaa tcaaaatgcc gtctgaagcg ttatccggct
      181 ttcagacggc atttttgtat ttaaagccgg gtaacgctca atacgccttt gacatcgccg
      241 aggccggcga ggacgcgcgg gaggtcgttg acttgtttga cttcgagcgt gaacctcatg
      301 ctggcttcca agtcgcggga ctgggtttgt acggcggtaa cgttgagttt gtggcgggcg
      361 agcgcgtcgg atacgtcgcg caaaagcccg gagcggtctt gggcgcggat ttcgatatcg
      421 acggcgaaca cttgcccttc ctgcaacgcc gcccaacttg cgtccagtac tttttcgggc
      481 gcgtgttcgg caaggtgtcg gaaagagggg caggttttgc ggtggacgga aatgccgcgc
      541 tcgcgggtaa cgaagccggc aatatcgtcg ggcggcgcgg gtttgcagca tttggcaagc
      601 gtggtcatca agccgtcttc gccgtcgatg agcacgccgg ttttgccacc ttttttgatt
      661 ttggactgtt tgacgatggt ggttgcgctg acgggcacgg gcggcggttc gttcagcgtg
      721 ccgcaggctt tttggatggc gcggttggaa atttcgcctt gtccgacggc ggtgtagagg
      781 tcttctggct ttttgtagcc gagattttcg gcaagctctt gcaggttggg tttgggcgtg
      841 agtttggcaa gctgcttgtc gagttggaca cggccttctt cgcgcacggt gtcggcgttt
      901 tgctggcgga tgtaggcgcg gattttgccg atggccttgc cggatttgac ccagccttcg
      961 taaagccagt tgacggaagg atgcccttct ttggcggtaa tgatttcgac gcgctgtccg
     1021 ttttcgagcg gggtggacag cggcacaatc tgcccttcga ctttcgcgcc ccggcagcgg
     1081 tcgccgatgc tgctgtgcag ggcgtaggcg aagtcgatgg gggttgcgcc cgttggcaga
     1141 gagaggactt tgccgtgcgg ggtcaaaaca taaatcgtgt cgttgaaaag ctcggttttg
     1201 aaggcggcgg cgaggtcttc cttgccgctt tccgccatat tttcgcgcca gtccaagagt
     1261 tggcgcaacc aggcgatttt ttgttcgtag gcggaatcgc ctttgccgcc ttctttgtaa
     1321 cgccagtggg cggcgacacc gaattcgttg aattggtgca tatcgaaggt gcggatttgc
     1381 acttccacac ctttgtcttc cgggccgacg atgacggtgt gcaaactttt ataaccgttg
     1441 cctttggggt tggcgatgta gtcgtcgaac tcgccgggaa tgggctgcca gaggctgtgg
     1501 acgatgccca gcgtggtgta acactcgggg acggtatcga ccagaatccg cacggcgcgg
     1561 atgtcgaaca ggccgtcgaa gctgagtttt ttcttcacca tttttttgta aatggagtag
     1621 atgtgtttcg gacggccggc gacttcaaag tggatattgt attttttgag ttccgtacgc
     1681 aggatatcga ggaagttttc gatgtattcg aggcgttcgg tgcgtttttc gtccaaaagc
     1741 agggcgattt cgcggtattt ttcgggttct tgatggcgga agcccaaatc ttcgagctgc
     1801 catttgagct gccacacgcc caagcggttg gcgagcgggg cgaagatgtc gagggtttct
     1861 ttggcgacgg cgcgtttttc ggggctgtcg ggggcgttgc ttaaaaattg cagggtgcgc
     1921 gtacgcatcg ccagtttgat taatacgacg cggatgtcgg taaccatcgc cagcagcatt
     1981 ttccgcatgg tttcggcttg ctgcgcgcgc tcttcggaag tggcgaggct gtccacccgg
     2041 gcgaagtggg tgagcttctg cacttcgtcc acacctttga ccagctcggc gacggtgctg
     2101 ttgcagcgct cggaaaccaa taggttccaa tcggggacgt agcgtccgat gtcggcaagc
     2161 agggtggcgg cgacggcatc gggcagcagg tcgagttcgt cgaccatttg cgccgcgccg
     2221 aggaagtggt cgggcagcgg ctcgccatac ggcgtggcgg catcggcagg ataatgttcc
     2281 tgcgccagcg accatgcggt accgatgagg tttttatcgt tgtccggcag agcggcgcag
     2341 tagctgtcga accattcgcg caattcttgc agggtcgcgc tttgcgtgtc ttgaatcggg
     2401 ctgatggcgg tcatgatggg atcttccgtt ccgataaaaa cgagttcatt gtaacagctt
     2461 atttatttac ggtgcatagg cgggaagata aacttgtttc gggcggcgcc gggtgttaag
     2521 gtagccggta ccgacaggtt gacgaggaat attttgatga aactgtatat ttacgaccat
     2581 tgcccgtttt gcgtgcgtgc gcggatggct gccgggctgt ttggtgctga cgttgaagaa
     2641 gtcgtgctgg caaacgacga cgaggcaacg ccgatcggta tgatcggtgc gaaacaagtg
     2701 cccgtcctgc aaaaagaaga cggttcgttt atgggcgaga gtttggacat tgtgtgccat
     2761 ttcgaccgag aaggccgtct gaaagacgaa gtccgccccg agatacaggc gtggctggac
     2821 aaggtaggcg gatacaacaa caaattggtt cagccacgct tgatcaaaat cgggctgccc
     2881 gaatttgtaa cgccggaagc ggtgaaatat ttcacagaca aaaaagagaa aagcatcggc
     2941 agcttttccg ccaatttgaa taaaaccgcc caataccttg aacggataaa cgcagatttg
     3001 caagagttgg agaatttgat ggacggtact tcagacggca tcaacggtgg aatcggtatg
     3061 gaggatattt tggttttccc cgttttgagg aatctgaccg tggtgcgcgg catcgcgttc
     3121 cctcggaaga cgatggatta tttaatcggt atgagtgaaa aatcaggcgt gccgctttat
     3181 ttcgacaggg cattgtaaaa ccaaaaaaaa gcggcagggc aatatgtcct tccgcgtttt
     3241 ttggaacgtt tatgccgtct gagagccagt cagacgaaca tctgcacaaa cgcaatcacc
     3301 agccgcacaa tcggtgcaat aaacgcaccc aaaaccccgg tcagcataag cagcaggata
     3361 atccacgtcc cataaggttc gattttgcgg aacgcttgcg aatatttcgc cggcaggaag
     3421 gtgtcgatga aaatgccgcc gtcccaaggc aggatgggga tgatgttgag cgcgaacaaa
     3481 atcgcattga tcagaatacc gtagtttgcc atttgagcca gcggcatctg atacgccccg
     3541 ccggcatacg gagttagcac caaaaccacg ccccacagaa cagccatcgc cagattcgac
     3601 agcgggccgg acgcggcaat gcaacgccat gcaaggcgcg ggttgcggaa gttgcgcgaa
     3661 tcgataggaa tcggacgcgc ccagccgaac aggaagggcg tgaacatcaa agtaagcagc
     3721 ggtacgatga ttgtgccgac caaatcgata tggggcaggg ggttcagtgt cagcctgccg
     3781 tattgttcgg cagtgttgtc tccccagtag cgcgccgtat agccgcgcgc cacctccctg
     3841 acggtaatgg cgagcagcac gggcaggaca gcaagcagaa acacgcccaa gtcaaaattt
     3901 tgaaacatgg ttttcctttg aacagatgtg gtgtgcagcc cgaaacggct gacagacaga
     3961 agcggcaata tatcatctaa cctgatttta ttggaataag aaatcagacg gaatcatttt
     4021 ttctgccggc ccgttatctg atgatttgct gtaataccgc gtttaaacac ggtttgccta
     4081 tgaatggagc aggcgttcac atgacaacaa attataaaaa ataggttgca aataggaatt
     4141 gttgctatta ttatgcccga attatggata aagaacgaat tttaaccccc gcagtcgtgt
     4201 tttccgtcgc actgctgcat ttggcaatag tggcattgct ctggcaggcg cacaagctgc
     4261 ccgtgataga gtcaggaaat gttatcgaat ttgtcgattt gggcgatttt ggcggagggg
     4321 gcggcgcacc cgaaggcgca ggcgcgcctg ccgcgcccga accgcaaccc gcgcccgatc
     4381 cgcccaaacc tgtcgaaccg cccaagccgg ttttgaagcc ggcggttaca aaaaaggcgg
     4441 atgcggatat tcagcaacct aaggaaaagc cgaaacccga agaaaaacct aagcccgaac
     4501 ccgaaccgga agcgaagcct gccccgaaac cggcggaaaa accggcagag aagccgtctg
     4561 aaaaacctgc cgaacattcc ggtaatgctt ctgccaaagc cggcagcgag cagggtaatg
     4621 gggaaggtaa gggaaccggt accaaaggcg acggaacggg gcgcggagaa ggcagcggta
     4681 aaggaagcgg cggtgccaaa ggcgaacacg gggaaggagc cggcggcagc ggcggcggaa
     4741 ccggtgtcgg cagcagcaaa ggcaatcctt tacgcgccaa cggcagcatc ccgcgcccgg
     4801 cctatcccgc actttctatg gaaaatgacg agcagggtat ggttgttttg agcgttttgg
     4861 tttctccggg cggtcatgtc gagtccgtca aagtggtgaa aagcagcggt ttttcccgtt
     4921 tggacaatgc ggcacgcaaa gcggcgcaaa acgggcattt tcaagccaat gcctggacgg
     4981 agtttaaagt ccccgtcaag tttgaattga attaggcggt tgtcgcggca gtttgcggca
     5041 gcttggatgt ttgattggtt ttttcagtgt tggaatcatt atgaatttga aattagtgtt
     5101 tgaatcgggc gatcccgtcc tgatcggtgt gtttgtgttg atgctgttga tgagtaccgt
     5161 aacgtggtgt ctggttgtct tgcgctgcat caagctgtat cgggcgcgca aagggaatgc
     5221 cgccgtcaaa cggcatatgc gcgatacttt gtcgctgaac gacgcggtcg aaaaagtgcg
     5281 cgccgtcgat gcgcctttgt ccaaactggc gcaagaggca ttgcagtctt accgcaacta
     5341 ccgccgaaac gaagcgtccg aactggcgca ggctttgccg ttgaacgagt atttggtcat
     5401 tcaaatccgc aacagtatgg cgcagattat gcgccggttt gattacggga tgaccgcgct
     5461 tgcctccatc ggcgcgaccg cgccgtttat cgggttgttc ggcacggttt gggggattta
     5521 ccacgccctg atcaatatcg ggcaaagcgg gcagatgagt attgcggcgg ttgccggccc
     5581 gattggcgag gcactggtgg cgacggcggc gggtttgttc gtggcgattc cggcggtgtt
     5641 ggcatacaac ttcctcaatc gcggcacaaa aatactgacc caggatttgg atgcgatggc
     5701 gcacgatttg cacgtccgcc tgcttaatca aaaggatagc tgattatggc atttggttcg
     5761 atgaattcca gcgacgattc tccgatgtcc gacatcaacg ttacgccgtt ggtggacgtg
     5821 atgctggtgt tgctgattgt gtttatgatt actatgccgg tgctgacgca ttccatccct
     5881 ttggaactgc cgaccgcgtc cgagcaggca aacaagcagg acaaacagcc taaagacccc
     5941 ctgcgcctga cgattgatgc gaacggcggc tattatgtcg gcggggattc tgcaagcaaa
     6001 gtggaaatcg gggaagtgga aagccgtctg aaagccgcca aggagcagaa tgaaaacgtg
     6061 attgtggcga ttgcggcaga caaggcggtg gaatacgatt atgtaaacaa agctttagaa
     6121 gccgcccgtc aggcaggaat taccaaaatc ggttttgtaa ccgaaaccaa ggcgcaataa
     6181 agttcgacgc ttgaacgaat gccgcctgaa agtctttcag acggcatttc ttatgtttaa
     6241 tcagttatat atagttgatt aaaacaaaaa tgagacgaca gagcaccgtc tccatttctg
     6301 cgcaggcggg aatacaaaca tctgattttt cttttatttt caaacattac tgaaatgggt
     6361 aagtctggag tcctgcctgc gcgagaataa tgaaaatgtg catttctaat ttttatagtg
     6421 gattaacaaa aatcaggaca aggcaacgaa gccgtagaca gtacaaatag tacggaaccg
     6481 attcacttgg tgcttcagca ccttagagaa tcgttctctt tgagttaagg cgaggcaacg
     6541 ccgtactggt ttttgttaat ccgctatacc cactgtaaaa acggagaagg acagaggacg
     6601 gcagtaaggt ttgccgtcct ctttttcaag ttgcggtgga atgccgcttg aaggtgtggt
     6661 ttaaacgttt cggcttgtaa tcagacgcgg gcgttgtcca gccgctctct gaagttttcc
     6721 agattcacac ctgccgcttg ggcgcggcgg atgatttcgt caaccggcat accttctgcc
     6781 gcccggcgga agccccacag gagggagcag cgcgtaccgg tccggcaata ggcaaggacg
     6841 ggatattcgg cttgtccgat gagttggcgg aaggtttcga catcgtgttt ttggatgtcg
     6901 cgtgcggtaa cgggttggtg atggaatccg gtaacgcctg cttgttccag ccactgtttg
     6961 atttgggcga agtcgggttg cgattcttct tcgcggtcgg ggcggttgca gatgacggtt
     7021 ttgatgccaa gttgcgcgat ttgttccgca tcggctttgg tcagttgcgg ggagatgtag
     7081 agatgttcgt caagtttgag aatcgccatt ttgtttcctt tgctttgcgg ttggtgttca
     7141 gacggcattt tgtgtgcaat gccgtctgaa agtttaaccg aacaaccagt tttccaacag
     7201 gttgcgatct gtgatgtgta ggatggcagt atccgccgcc gttgcctgaa ccgtcaagac
     7261 ggtttgacgc aatatgcctg cacggaagaa gtggatattg atttttgccc tgacgtggta
     7321 tcgggcccat tgtgcggcaa agtcggtgca ggcataaccg tctaaagcaa tgattttgtc
     7381 ttgcgggcac agtgccgcag attccgcact tccgccgttg aagacatggg tcaggacgat
     7441 atggtcggcg ttttgtttga agcgtgcgcc gaaatcgccc gtcggcggga tggggaaaat
     7501 gtgttccgca tatccgccgc cgtgttgtcg gggaagcggc aggaaggtca gtttcacgcc
     7561 tgtggttgcc aggcattcgg caagcggcaa atcttcggtg ctgtacaatg ccgtctgaaa
     7621 aaaatctgtc aaatccaagc cggtaatttc ctgacaacgg atttgccagt gtttttccgg
     7681 aatacccgag tgtgtgtccc tccactcccg atagagtttg tccattaccg tatcaaggga
     7741 atgtctgccg ttgcttcggt tgcgtattat cagatcaagg cacaatgcgg caagtgcgcc
     7801 tttttggtag tagctgacga tggcgttggg gctgttttca tccggtttgt aaaatttgtt
     7861 ccacgcggta aaactcgatt ccgccaaggt ctgcctcaaa cggccgcggg tttgttgtac
     7921 gcgcgtaatg ccttgtgcta gcaggtttaa ataaaattcg ggcgagatgg tgcggctgcg
     7981 tgccaaaaac aaatcgtcgt aataggatgt aatgccttcg aatgcccata gttgttcggt
     8041 atagttttct ttgtcgaggt cataaggggc gaatgcggca ggtttgatgg atttgacgtt
     8101 ccacgcgtga aaatattcgt gggagaaaag tccgagcaat gtggtgtagg catcgtcggc
     8161 atcggtcata ccgtacggcg gaaggctgtg gcgatcggcg agcagtgcgg tgctgtcggt
     8221 gtgttccaaa ccgccgtaaa tatggtcgcc gacgtggagc aggaacaaat atttctcaaa
     8281 tggggcaggg gaggagaaca tcgccagttc tgtttcgcag atttttttga tgtccgaaac
     8341 cagcctgtcg cggtcgaaat cgggatatat gccgtttaag gcaatcgtgt gcggaatgcc
     8401 tgccgcctca aaatctaaaa attcaatcaa gcccatctcg acaggtcggt caatcaattc
     8461 gtcataagat gccgtctgaa agacaagcct ccctgtttcc ggcagcgttg tggcaatacg
     8521 ccattcgggt ggaatgcctg tcaattccaa gcggtgcggc agcgtttccg ttccttcgac
     8581 tttcaaaaac aggcacgatc cgtcaaaaaa accgcgttct gtcgtcagga aagaacctcg
     8641 aaccgacaaa tcgaatgcat atacggtgta gcggatttgc cactcgccgc gtacggcggc
     8701 agtgtgccag cggttttttg caatttgttc gagcggcatg gacgtgccgt tgcaggatgc
     8761 atggatagaa gtgatgtggc gggaaaaatc ccgaatcaga tagctgcccg gcacccaatt
     8821 cggaaggctt atttctatag gaaaatcatt atcttgtgtg aatttcagca ggatgtgcca
     8881 ttcatggtct agaggggacg gggctatttt ataatgaatc ataactatca aatatatcgt
     8941 taaacgggat ggattaaatg cctttaaaat gcagcttaac ctacatcaat aaagtgccac
     9001 aaaaagggaa aattggaaac aaaagcaatg gcggccgggt tgtaatttat tataacaata
     9061 tgatttatat gtaataaaaa acagattaag caaagggttc gccggcggcg cgaatgtaat
     9121 tccgcttggc gtaataaggc agatatgtta aatttagaaa aaattacgct tgcccggatt
     9181 gcaccttaaa caggctgcgg aagcggcagg ataaaaatct ttattcccat aattcatgga
     9241 gactctcatg gacacacaaa cttacaacta caaagtggtg cgccagttcg ccatcatgac
     9301 tgtagtttgg gggattgtgg gcatgttggt cggcgttatc gtcgccgccc agctttttgc
     9361 tcctgccctc gacttgtcta atatcggacc ttggttccac ttcggccgcc tgcgtccgct
     9421 gcacaccaat gcggttattt tcgcatttgg cggttgcggt ctgatcggca catcatacta
     9481 cgttgtccaa cgtacttgta atacccgtct tttcggcggt tggctgccgg catttacctt
     9541 ttggggttgg caggcggtaa tcgttgccgc cgtcgtcagc ttccctatgg gttggaccca
     9601 aggtaaggaa tatgccgaac tggaatggcc gattgacatt ctgattactt tggtttgggt
     9661 ggcttacgcc atcgtattct tcggtacgat tgccaaacgc aaggtaaaac acatttacgt
     9721 tgccaactgg ttctacggcg gctttatttt ggcggtcgca cttttgcaca tcgtcaacaa
     9781 tatcagcatc ccggccggtt tgatgaagtc ataccccgtc tattccggtg cgattgatgc
     9841 tatggttcaa tggtggtacg ggcataacgc cgtgggcttc ttcctgactg ccggcttctt
     9901 gggtatgatg tactatttcg tacccaaaca agcagcccgc cccgtttact cctaccgcct
     9961 gtccgtcgtt cacttctggg cgttgatttt cacctatatg tgggcgggtc cgcaccatct
    10021 tcactacact gcgctgcctg actggacgca atctttgggt atggttctgt ccttgatttt
    10081 gttcgcaccc tcttggggcg gtatgattaa cggcattatg accttgtccg gcgcgtggga
    10141 caaactgcgt acagacccga ttcttaaatt cctgattgta tccttgtcct tctacggtat
    10201 gtctaccttt gaaggcccga tgatgtcgat taaaacggtc aatgcattga gccactatac
    10261 ggactggacc gtcgcgcacg ttcatgcggg tgcgttgggc tgggtaggct ttgtaaccat
    10321 cggttccgtc tattacatga ttccccgtct gttcggcaaa gagcagatgc acagcaccaa
    10381 gctggtagag gcgcatttct ggattgcgac tatcggtgtg gttctgtata tcgccgcgat
    10441 gtggattgca ggtgtgatgc agggtttgat gtggagttct ctgaacgatg acggtacgct
    10501 gacctactcg tttgtcgaat ccgtaaaacg caccatgcct tactacatga tccgttttgc
    10561 aggtggttta ttgtatctga gtggcatgtg cattatggcg tacaacgtgt accgcacagc
    10621 catcggtggt aaagcagtcg atgccgaaat ccctgcggtt tcccaaacac agcaccacta
    10681 aaatactaag aaagataggc taccaaaatg aaattacaac aattggctga agaaaaaatc
    10741 ggcgttctga ttgtgttcac gctgcttgta gtcagtgtcg gtctgttgat tgaagttgtg
    10801 cccttggcct ttaccaaggc ggcaacacag ccggcgccgg gcgtgaagcc ttacaatgcc
    10861 ctgcaggttg ccggacgcga tatttacatc cgtgagggct gttacaactg ccactctcaa
    10921 atgattcgtc cgttccgtgc ggaaactgag cgttacggtc attactctgt tgctggagag
    10981 tcggtttacg accatccgtt ccaatggggt tccaaacgta ccggtcctga tttggcacgt
    11041 gtgggcggcc gctattccga cgaatggcac cgcatccacc tgctgaatcc ccgtgatgtc
    11101 gtgcctgagt ccaatatgcc ggcattcccg tggcttgcac gcaataaagt cgatgtcgat
    11161 gcaaccgttg ccaacatgaa ggctttgcgt aaagtaggta ctccttacag tgatgaggaa
    11221 attgcgaaag cgcctgaggc tttggcaaac aaatccgagc tggatgctgt agtcgcctat
    11281 ctgcaaggat tgggtctggc tttgaaaaac gtaaggtaac atcatggata ttaacggtat
    11341 tcgcgctctt ttcacggtat ggatctttat ctgtttcctg ttagtactct atatcgtctt
    11401 caacaggcgg aataagaaaa actacgatga tgccgcaaac agcatttttg ctgaaaacca
    11461 agatgcgcaa gataagaaaa gcgaaaaccg ttaatattgt gataacggag caaaacaatg
    11521 aacgcaacat cccaatttac cagtaatttc tggaatatat atattgcagt tattgtctta
    11581 ctgagcttta tcgctttggc ttggctgctg ctgtctcaaa atgttgtcaa acgtccgaag
    11641 aagggcgaag aagtacaaac tacgggtcac gagtgggacg gcattgccga atacgacaat
    11701 ccgctgcccc gctggtggtt ttggctgtgt gttttgacgt ggctgttcgg tatcggttat
    11761 ctggttatgt atccgggtgt cggcgactac aaaggtctgc tgaaatggac cagccataac
    11821 caatatgaaa aagaggtcaa aaaagccgat gagcaatacg gcaaactgta tgccaagttt
    11881 gcggatatgc cgattgaaaa agtggcaaaa gacccacagg ccaagcaaat cgcccaaaac
    11941 ctgtttaaca cttattgtat ccagtgccac ggctctgatg ccaaaggttc taaaggtttt
    12001 ccgaatctga ccgatagcga ttggttgtgg ggcggcgatc ccgataaaat ccacgagacc
    12061 atcgaaaaag gccgtgttgc gactatgcct gcctggggtc ctgctttggg cgaagaaggc
    12121 gtgaaagacg ttgcccatta tgtgatgtcc ctttcaaaac ccaaaggtca gtatgatgag
    12181 gaacgtgccg cgcgcggaca agccttgttc agcggtccgc ctgccaactg tttcacttgc
    12241 cacggcgata agggacaagg tatccaaggt ttgggtccga acctgactga tgacgtgtgg
    12301 ttgtggggcg gtacgcaaaa atccattatc gaaaccatta ccaacggtcg cagcagccaa
    12361 atgcccgctt ggggacattt cttggataaa gacaaactgc atatcatgac tgcttatgta
    12421 tggggtcttt ccaataaaga cggtaaagct ccggtgaaaa aagccgagcc tgcaccggcg
    12481 gcagaacccg caccatctgc tcctgcagaa gcagcacaag ccgcttctga agccaaacct
    12541 gccgcagcag aacctaaagc cgaggaaaaa gccgcacctg ccgccaaagc ggatggcaaa
    12601 caggtttatg aaaccgtttg tgccgcctgc catggcaatg caattccagg tattccccat
    12661 gtaggcacca aagccgactg ggccgaccgc atcaaaaaag gtaaggacac gttgcacaaa
    12721 cacgcgatcg aaggttttaa tacgatgccc gcaaaaggag gtcgcggcga tttgagcgat
    12781 gatgaggtca aagctgcggt tgactatatg gtcaaccagt caggcggtaa gttctaaccg
    12841 ttgttttccg actttgtcgg atgcagaaat ccggattcat atcgaatccg gattttttat
    12901 tttgaacgga aatcctgttt ttgatttatg ccgtctgaaa gtggggattt tgatgcttaa
    12961 gcagtcagac cgactgatat ttttgtatgg tttaaagaat ggtgcagttg ggtgtcggac
    13021 agcattgttt gatgctttca gacggcatat aggtttgttt cttcaaaatt gaatcttttt
    13081 tcatatgttg caaaaaaatt gattttagtc ataaagaatt agtctttatt gtttataatc
    13141 gcgcatcatc acgatagcct taatttcaaa aggacgatta atggatacac aaatcaaaac
    13201 tgaagccggt cagcagaata accggcatta tctgagcgtt tggaggtggc atttttatgc
    13261 cggtttattg gttatgccct tcttgaccct gcttgccgtt acaggtttgg gtatgctgtt
    13321 gtttgccaat attaccggta aagagggaga gcgtattcac gttttccctc aggcagtggt
    13381 gcaacccctc tctgttcagg cagaagcggc acgcggtgcc attaatccgg agacttcgtc
    13441 cgtcgtccaa tatattgcac cgcgtgccga tgatatggtt gctgtgttcc gtgtcaacaa
    13501 tgagggtaag gcaaccatgg ttgcggtcga tccttatacg gcaaaagtgg tcaacaccat
    13561 gccgcgcaat cagggttggt atcacactat ggatgaaatc cacggcgata tgatgctcgg
    13621 tgcggcaggc gattatcttt tggaaacggc agcttcactg accattatta tggttgtcag
    13681 cggcttgtac ctttggtggg cgaaacagcg cggcattaaa gcgatgctgc tgccgccaaa
    13741 aggcagggcg cgttcttggt ggcggaatct gcacggcgcg tttggaactt gggtatcgtt
    13801 gattttactg ttgttctgcc tgtcgggtat tgcttgggca ggtatttggg gcggcaagtt
    13861 cgtgcaggct tggagtcagt tcccggccgg caaatggggt gtcgaaccga accccgtttc
    13921 agtcgtgccg acccacggcg aggtattgaa tgacggcaag gttaaggaag tgccgtggat
    13981 tttggagctt acgcctatgc ctgtctcagg gacgactgtg ggtgaaaacg gcattaaccc
    14041 cagcgagccg atgacattgg aaaccgtcga ccgtttcgcg cgggaaatcg gtttcaaagg
    14101 gcgttatcag ttgaatttgc ccaaaggcga ggacggggta tggactttgt cgcaggattc
    14161 tatgagttat gacatgatca gcccgtttgc cgaccgcacg gtgcatatcg accagtacag
    14221 cggcaagatt cttgccgaca tccgttttga cgattacaac ccgttcggca aatttatggc
    14281 ggcaagcatt gcgctgcata tggggacttt gggctggtgg agcgtgttgg cgaacgtcgt
    14341 gttctgcctt gccgtgattt ttatcggcat cagcggctgc gtgatgtggt ggaaacgccg
    14401 tccgtccggc gtggcgggca ttgttcctcc ggcgcaaaaa atcaaactgc ccgtctggtg
    14461 ggcgatggca ttgccgctgc tgttgattgc actgcttttc ccgaccgcgc tgcttgccat
    14521 tgccgtgatt tggctgttgg ataccttgct gctgtcgcgg attcctgtgt tgaggaaatg
    14581 gtttaaatga cccgatgccg tctgaagcgt tcagacggca ttttgtttgc gggcggcttg
    14641 cccgagaagc tatataattc cgcatcactg tcattttgaa aaagcaagcc atgttttacc
    14701 tgtaccaatc caaccgcctc gaatcgctcg ctgcactgtt tgcccgcatt caaaaagtca
    14761 aaccgctgaa atgtgccttg cagcccgaac agattgttgt gcagagccag gggatgcgcc
    14821 gctacctcaa cacctgcctc gcccgcgatt tgggcgtggc ggcgaatttg gcgttcagcc
    14881 tgcccgccgg cctgacgtgg aagttgatga aaaaactgat tcccggtatt ccggaactca
    14941 gcccgtttgc acccgaagtc atgcgctggc ggctgctgga tttgttccgc agcgcagaat
    15001 ttcaaaacgg agcggaattt gaagatgtga ggaatgtgct gcaagactat ctgggcagcg
    15061 gagaatcggc ggattaccag cttgcgggac agcttgcaga catattcgac caatacctcg
    15121 tgtaccgtcc tcagtggata gacgcttggc agcagggcag gatactcggt ttgggcgacg
    15181 acgaaatctg gcagtccaaa ctgtggcgtt acctcgacga cggcaggcag agcgcgccgc
    15241 accgtgtcgc gttgtgggaa aaactgcttg ccgcgttgga caaggataag ctgcccgagc
    15301 gttatttcgt gttcggcatt tccacgatgg cgccgatgta tttgcagctt ttgcacaagc
    15361 tgtccgaaca ttgcgacgtg ttcgtgttcg cactcaatcc gagcgggatg cattggggca
    15421 acgtcatcga agcggcgcaa atcctcaaag gcggcggcga tcccgatctg acgcaggcag
    15481 ggcatccgct gcttgcctca ttgggcaagc aggggcgcga ctttttcgac tttttgaacg
    15541 aaatggaaat agaaggcgaa acgccggtat ttgaagaagg cggacgcgat acgcttttgc
    15601 acgccctgca aaccgatatt caaaacctga aaatgccgtc tgaaaatgtg ggaagcgtca
    15661 acacgggcga cggctcgata cgcatcgtct ccgcacacag ccctttgcgc gaattgcaga
    15721 tactcaaaga caagctgttg aaaattctgc atgaacatcc cgattggcag ccgcatgata
    15781 tcgccgtatt aaccccgaac atcgaaccct atacgccttt tatcgaagcc gtgttcggac
    15841 aggcgcagcc cggcgcgcag gcattgccgt attccgtttc ggacgtgaaa atcagccgcc
    15901 ggcaaccgtt tttttatgcg ttggagtgcg tgtccgactt gctggaaagc cgttttgaag
    15961 tcggcaaagt gctcgcgctt ttggaaaccg cgcccgtgtt gcgccgtttc ggactgaccg
    16021 gggacgattt accgcttttg cacgacatgg ttgccgattt gaacgtccgc tggggtttgg
    16081 acggagaaat gcgcggcggc acggatcagc tgtttacctg gaagcaggcg gcagaccgta
    16141 tgatattggg ctggatgctg cccgaaggcg gcaacccgat gtggcaggat gtcagcgcgt
    16201 ggtatgccga cgtgaatcaa accgccatgt tcggacgttt tgccgccttc ctcgaaaccc
    16261 tgtcggatat tgcccggata tggcggcaac ctgcgacggt cggcgaatgg gtggcgcgtt
    16321 gccgggattt gcttgaaaca ttgtttcaag ccgggcccga tgaccaaaag gcagtccaaa
    16381 acctcgaaaa cgaatgggtc aaatggcagg aagagagcac tttggctggg tttttcggac
    16441 agttgccgca acaaaccgtc atccgccata tccgccgctt cctcgacagc gaaagcgagg
    16501 caggcttttt acgcggcggc atcacctttt gcagtatggt gccgatgcgg agcctgccgt
    16561 tcaaagtcat ctgcctgttg ggtttgaacg acggagattt tccccgcaat accaaagccg
    16621 ccgtattcga cctggttgcc aaacatcccg ccaaaggcga ccgcgcccgc cgcgacgacg
    16681 accgctacct gttcctcgaa gccctcatca gcgcgcgcga aatcctgtat ttgtcctaca
    16741 tcgggcgcga catccgcaaa gacgaagagc ttgccccgtc ttccctgttg ggcgaactca
    16801 tcgacacagt tgccgccatg accggcacaa acagccgcca acttgcacaa aattggatag
    16861 aacagcatcc gctgcaagcc ttctcgcgcc gatatttcca agaaggcgga cgttcagacg
    16921 gcatattcgg cacgcgtacc gactacgccg ccgcgctcgg acaaacgccc gaaccgccgc
    16981 gccctttttt cgaccaaccc gtagaaaacg ccgaacccgt tgccgaaatc ggacaggacg
    17041 aatttatccg cttctggcgc aaccccgtca aagtatggct tcagcagcag cttgcgtgga
    17101 gcgaacccca tatcggcgaa gcctgggagc ctgccgaacc cttcgagccg caacacgcgg
    17161 agcaaatcgc cgaaacctat atcggggcgc gccgcgaagg acaagatttt tcccaaaccg
    17221 ccgcccgtat cgcggcggaa agcctgctgc cttccggaga attgggaaaa ctttggcagc
    17281 agtccttcca aaccgccgcc aaacaaatcg acacggcggt tttaaacagc cccaaactgc
    17341 ccccgtttcc atacgccata ccgtcggacg ggcaaatcct gaaaggcagc ttgggcaatc
    17401 tgtaccgctg cggtcaggtg ttttatgcct acggcaaacc caatgcgccg caacgtatcg
    17461 cttttttgtt ggaacacctg atattttgcg ccgttatgcc gtctgaagcc gaaacgcggc
    17521 aaacctttat cgtcaaatcc ggagaaaccg aagtattggc ggaaatcgcg caagacaggg
    17581 cattgcagct gttgtcggaa tggattgtat ttttcaatat cgggcaaaac cgcccgctgc
    17641 cgttttttgc caaaacctcg cttgccgccg ccgaagcgtt tgcccaaaaa caagattggg
    17701 aagccgccct gaaaaaagcc caaaccgcct attacggcag caaagtcagc aaagggcaga
    17761 aagactatac cgaagtcgcc ctcgtgttcg gcaacgcaag ccaaaacccg ctcgaacagc
    17821 ccctgtttga aaacctcgcc cgcctgctcg ccgacacact tgccgcagcg gaaaagaggg
    17881 aagggaccgg agaagcctga cgggatggaa aggcaaatac cgtctgaaaa cagaaaaaag
    17941 gttttcagac ggtattttgt ttatcgtgtt taaggcaccg gatagaatgt gggtgcgccg
    18001 ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga ataaggcgat ccatgcaatt
    18061 aagaagaaag cggaatacgg caacatcata gaaatcagcg tgcctacgcc cgcatctttt
    18121 ttgtatttga ttaccgtcgc cataatcagc ccgaaataac tcatcatcgg cgtaataata
    18181 ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga cttcgggcgc gtaaccggcc
    18241 aacatcagca tagggacgaa aatcggtgcg gttaccgccc attgcgcgga agcggagcct
    18301 atcatcagat tgataaaagc acaaattaaa ataaaaccga taaacaacac gctgccggcc
    18361 aagccgactt cttttaagaa caccgcccct ttaacggcaa tatattgccc aatattcgtc
    18421 caattaaaaa atgcgacaaa ctgtgcggca aaaaagatga tgaccaaata aagtcccaaa
    18481 gtactcatcg attcggccat cgcattaacg acttcccgtt cgccgcgcaa acttcgggtt
    18541 atccggccat aaacaatgcc cggtagcgca aacaacaaga aaataaaaac aacaatcgat
    18601 tttaaaaacg gcgaaccggc aaccaatcct gtttcaggat gacgcaaaat accgtcggca
    18661 gggacgatgc tccaagccaa tagggcggat aaagcaataa acaccacgcc tgcccaaatt
    18721 aatcctttat attccaaagg cgtgatttca ttggaatgcc ggatgtcttt ttcttcttgt
    18781 gacaaatctg actgataagg gcccaattgc ggttcgacga ttttttcagt aacaaaataa
    18841 ccaatcaaag caatcacaaa cgtactggct gccataaaaa accagttggc ttcagggcct
    18901 acgacgtagt cgggatggat gatttgcgcc gcctgttggg taatgcctgc caagagcgga
    18961 tcgagtgtgc ctaagaacag attggccgaa taaccgccgg aaacgccggc gaaagccgca
    19021 gccagaccgg cgagaggatg gcggccgagc gaatgaaaga tgacggcgga caaagggatt
    19081 aggacgacat agcccaattc agaagccgta ttggataaaa tccctgtaaa aacaaccata
    19141 aaagtagt
//
LOCUS       DAXDSD010000038        18417 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXDSD010000038 DAXDSD010000000
VERSION     DAXDSD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18417
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.038"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(223..1440)
                     /locus_tag="ACFB2Q_001006"
     CDS             complement(223..1440)
                     /locus_tag="ACFB2Q_001006"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC0918050.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVSL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(1642..2370)
                     /gene="rph"
                     /locus_tag="ACFB2Q_001007"
     CDS             complement(1642..2370)
                     /gene="rph"
                     /locus_tag="ACFB2Q_001007"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC0918051.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(2452..2916)
                     /locus_tag="ACFB2Q_001008"
     CDS             complement(2452..2916)
                     /locus_tag="ACFB2Q_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC0918052.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3133..3909
                     /locus_tag="ACFB2Q_001009"
     CDS             3133..3909
                     /locus_tag="ACFB2Q_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC0918053.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            3913..4044
                     /locus_tag="ACFB2Q_001010"
     CDS             3913..4044
                     /locus_tag="ACFB2Q_001010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918054.1"
                     /translation="MPSETGFRRHLNKRGNQAVGSEKPTSARLKPYSQSTPFQNICI"
     gene            4154..5008
                     /locus_tag="ACFB2Q_001011"
     CDS             4154..5008
                     /locus_tag="ACFB2Q_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC0918055.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKIVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            5049..6257
                     /gene="pncB"
                     /locus_tag="ACFB2Q_001012"
     CDS             5049..6257
                     /gene="pncB"
                     /locus_tag="ACFB2Q_001012"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC0918056.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFGIPEPRTP"
     gene            6349..8067
                     /gene="argS"
                     /locus_tag="ACFB2Q_001013"
     CDS             6349..8067
                     /gene="argS"
                     /locus_tag="ACFB2Q_001013"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC0918057.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGVAKTAQPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVADDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKAGRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
     gene            complement(8003..8447)
                     /locus_tag="ACFB2Q_001014"
                     /pseudo
     CDS             complement(8003..8447)
                     /locus_tag="ACFB2Q_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257883.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8643..10061
                     /locus_tag="ACFB2Q_001015"
     CDS             8643..10061
                     /locus_tag="ACFB2Q_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC0918058.1"
                     /translation="MNIHALLSEQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWPAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAAMRL
                     SEPKSQKILFIVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQKIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
     gene            complement(10514..11260)
                     /locus_tag="ACFB2Q_001016"
     CDS             complement(10514..11260)
                     /locus_tag="ACFB2Q_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC0918059.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(11244..11423)
                     /locus_tag="ACFB2Q_001017"
     CDS             complement(11244..11423)
                     /locus_tag="ACFB2Q_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918060.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(11439..12110)
                     /locus_tag="ACFB2Q_001018"
     CDS             complement(11439..12110)
                     /locus_tag="ACFB2Q_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC0918061.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(12179..12850)
                     /gene="rpiA"
                     /locus_tag="ACFB2Q_001019"
     CDS             complement(12179..12850)
                     /gene="rpiA"
                     /locus_tag="ACFB2Q_001019"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC0918062.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(12927..13409)
                     /gene="ispF"
                     /locus_tag="ACFB2Q_001020"
     CDS             complement(12927..13409)
                     /gene="ispF"
                     /locus_tag="ACFB2Q_001020"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC0918063.1"
                     /translation="MTNIRIGQGYDVHQLIEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(13441..14130)
                     /gene="ispD"
                     /locus_tag="ACFB2Q_001021"
     CDS             complement(13441..14130)
                     /gene="ispD"
                     /locus_tag="ACFB2Q_001021"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC0918064.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(14127..14861)
                     /gene="dnaQ"
                     /locus_tag="ACFB2Q_001022"
     CDS             complement(14127..14861)
                     /gene="dnaQ"
                     /locus_tag="ACFB2Q_001022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC0918065.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEEPTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(15017..16327)
                     /locus_tag="ACFB2Q_001023"
     CDS             complement(15017..16327)
                     /locus_tag="ACFB2Q_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC0918066.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYSVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(16317..16502)
                     /gene="ccoS"
                     /locus_tag="ACFB2Q_001024"
     CDS             complement(16317..16502)
                     /gene="ccoS"
                     /locus_tag="ACFB2Q_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC0918067.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(16502..16801)
                     /locus_tag="ACFB2Q_001025"
     CDS             complement(16502..16801)
                     /locus_tag="ACFB2Q_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918068.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
     gene            17152..18348
                     /locus_tag="ACFB2Q_001026"
     CDS             17152..18348
                     /locus_tag="ACFB2Q_001026"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC0918069.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETAHFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         4307 a         5439 c         4585 g         4086 t
ORIGIN      
        1 gaatccgttt tttgagtttc gcttaagttc aataaatcct gaaactttaa tctcgccgtg
       61 aaaatgaatt ttctatttca atttgtcata tatagttaat ttttttaggg tattttattt
      121 tttgttccaa agtcaaaata tgccgtccga acgttcgggc ggcagacaaa acggcactgc
      181 ccgataaagg cagtgccgtt gtccgtttta aaccgtgaaa catcagccca aatcaaaggc
      241 tttatgcaat accctggttg ccagttccat gtatttttcg tcaatcaata cggaaacttt
      301 gatttcggag gtggaaatca tttggatgtt gatgccctct tcggcgagcg tgcggaagat
      361 tttggcggct acgccgacgt gcgaacgcat acccaaaccg actgcggaga ctttgcacac
      421 ggtgtcgtcg ccgtcgatag aagctgcgcc gatactgtct ttacgttccg acaggatttc
      481 caaagtctgt ttgtaatcgc cgcgcggtac ggtgaaagaa aaatcggttg tgccttcgct
      541 gccgacattt tggataatca tatcgacttc gatgttggca tctgcgaccg cgcccaaaat
      601 ctgataggcg acgccgggct tatcgggcac accgcgcacg ttgatgcggg cttggttttt
      661 atcgaatgcg ataccggtta cggcagctct ttccatgttg tcgtcctctt caaaggtaat
      721 taaggtgccg ttgccgccgt cttgcaggct gctcagtacg cgcaggcgca ctttgtattt
      781 tccggcgaat tctactgaac ggatttgcaa aactttcgaa ccgaggctgg ccagttcgat
      841 catttcttca aatgtaaccg tatccatacg gcgcgcttcg ggtacgacgc gggggtcggt
      901 ggtgtaaacg ccgtctacgt cggtatagat ttggcattcg tccgctttga gggctgcggc
      961 aagcgcaacg gcggaagtgt cggaaccgcc gcgtcctagc gtggaaatat tgccttcact
     1021 gctgatgcct tggaagccgg caacgatgac gactttgccg gcggcgaggt cggcgcgcat
     1081 tttttcgtca tcaatgcttt ctatgcgggc tttggtgtgg gcggtatcgg ttttgaggga
     1141 gacctgccag cctgtgtagc ttttggcatc cacgccgata tccttcaatg ccattgccaa
     1201 aaggccgatg gttacttgtt cgccggtagc taagacgacg tccagctcgc gcggatcggg
     1261 atgctcctgc atttcgtgcg ccaacgcaac cagacggttg gtttcgccgc tcatggcgga
     1321 tacgacgacg acgatatcgt gtccttcggc gcgggcttta gcgacacgtt tggccacgtt
     1381 tttaatgcgc tcgggcgagc ctacggatgt gccgccgtat ttatgtacga ttaacgccat
     1441 gtttcatgct ttcttgtgtg ggattggtcg ggcagcatgg tttgctggaa aagcggctat
     1501 tattactatt ttttacatga agttcaagaa cggactgcgc tttcccgcct gccgtttgac
     1561 agcggtcagc gaaaaacctg ttctttcaga tttttgacaa aatgccgtct gaacggtttt
     1621 cagacggcat acggacagta attaagcaac ggacaacgca ttttgctgat aacgcagcag
     1681 ttcgcctatg cctttttgcg ccagtgcaac cagtttgccc aactcgtcca aactgaatgg
     1741 cgcgccttcc gccgttccct gtatttcgat gattttcccc gatgcggtca tgacgatgtt
     1801 cacatcactg tcgcagccgg agtcttcggg ataatccaaa tccaaaagcg gcacgccgtt
     1861 cactacgccc gctgatacgg cggcaacagc ttcaaggatg gggttttcac tcaaaatgcc
     1921 gtctgaaacc agtttgccga cggcgatttg cagcgcgaca aacgcaccgg taatcgaagc
     1981 cgtacgcgtg ccgccgtccg cctgaatcac atcgcaatca atcaggattt gtcgttcgcc
     2041 gagtttttcc atatccacga ccgcgcgcag cgaacgcccg atcaagcgtt ggatttcttg
     2101 tgtgcgtccc gactgtttgc ccgccgaagc ttcgcggcgc atacgcaaag cggttgaggc
     2161 aggcagcata ccgtattccg ccgttaccca gccttggttt ttgccgtgca gaaacggtgg
     2221 ggcattttcg tccacggaag cggtacaaat cactttggta ttaccgcatt caataaggca
     2281 cgaaccgtcc gtatgcggca ggaaatgagg ggtgattttg atatcgcgca ggctgtcggc
     2341 ggcgcgtgag gtgcggatat aatcaggcat actgccctcc cattaaaaac agataaatta
     2401 aaaagcctta aatatgaaaa atcacattta aggccttcaa actgaaaatt tctacgcctc
     2461 ttcggctttg ctacggataa tcaaaagcgg caggtggctt tggcgcatta ccgtttcggc
     2521 gaaactgccc atcaaaaggt gcatcagccc ggtgcgcccg tgcgtgccca acaccagcag
     2581 gccggcaccg ttttcatcgg cataatcgac caaatcctgc gccatttcgc gcgcaccctt
     2641 attggcaacc agcaggtgtt tgacggtatt tcccacaccc agttcccggg cggtgcgctc
     2701 ggcggcatcc aaaacctcgt tgccttgcgc gacggcggcg gcctcgtaac tttcgtgttg
     2761 caaaaattcg ggggcgagcg ccatatattc ggcagggttg gcaacgtgta ccaaagtcag
     2821 acgcgcaccg ttgaccccgg caagctcggc ggcgtgtttc agggcattga tggacgtttc
     2881 actgccgtca acggcaacga ccagatgttt gtacatatcg tattctcctt ttgcaccgtc
     2941 tcacggtgcc gtctcttcag gtttgcgcaa aggcagtctg cgccgtttca ttatagaccc
     3001 gccgtcgggc tttatacaac agccgaacag cctgactgct ttccagtata atatgccgct
     3061 ttcgtgcagt caggcatttt ttgccggctt tcgttcactt tttgatttga cgcaatcttg
     3121 caggattcga ccatgtccga caacgctttg acctcttcgc gacgcttcgg cggcatcgcc
     3181 agactctacg gagactctgc cttgacgcac ttttcacagg cacacgtctg cgtggtcggc
     3241 gtgggcgggg tcggctcgtg ggcggtcgag gctttggcgc gcacgggcat cgggcgtttg
     3301 actttgattg atttggacaa cgttgccgaa tcgaatgtca accgccagct gcacgccctg
     3361 accggcgact tcggcaaagc aaaagttacc gccttgcgcg aacgtattac acaaatcaat
     3421 ccgcaatgcg aagtatttga aattgaagat ttcgttaccg aagacaattt gccggaatac
     3481 ttcggaaaag gttttgattt cgtcatcgac gcgatcgacc aagtgcgcgt caaagcggca
     3541 atggcggctt attttgtgga acgcaaacaa ccgtttgtcc tcagcggcgg cgcgggcgga
     3601 cagaaaaatc ccgccctgat tcaaaccgcc gatttaagcc gcgtaaccca cgacccgctg
     3661 cttgccaacc tgcgctacac cttgcggaaa cgctacggct tcagccgcga tactaaggaa
     3721 aaaatgcgcg tgccgtgcgt gtattcgacc gaaaatatca tgccgccgca atcgggcgca
     3781 gcgtgttctg ccgatgccgc gccgcagggc ttgtcatgcg ccggctatgg cgcaagtatg
     3841 ctcgttaccg cttcgttcgg actgtattgc gcacaggcgg cggtggaaca catcgcgggc
     3901 aaaaaataag caatgccgtc tgaaacagga ttcagacggc atttgaacaa acgcggcaat
     3961 caagctgtcg gttcagaaaa accaacatcg gcacgcctaa aaccgtattc acaatctact
     4021 ccttttcaaa atatttgcat ttaaaagccg ttataatgcc gtccgaacat ctgcccgacc
     4081 acattatgcg tgaatgccgg cagattgttt tcttttgtaa acttatatta aaattcactt
     4141 accaattcac gccatgccgc ccatccctgc cctatccgca ccatccgagc acactgtcgc
     4201 atgggtattc ggccaacccg ttaccgattt gccgcaggat ttgtttatcc cgcccgacgc
     4261 gttgaaaatc gtgttgggca gcttccaagg ccctttggat ctgctgctct accttatccg
     4321 caagcagaac atcgatgttc tcgatattcc gatggtggaa attaccgggc agtatctgca
     4381 ctatattgcc caaatggaag cctatcagtt tgatttggcg gcggaatatc ttttgatggc
     4441 ggcaatgctg attgaaatca aatcgcgcct gctgctgccg cgtaccgaag ccgtcgaaga
     4501 cgaagaggcc gacccgcgtg ccgagttggt gcgccgtctg cttgcctacg agcaaatgaa
     4561 actggcggcg cagggtttgg acgcgctgcc gcgtgcggga cgggatttcg cgtgggctta
     4621 cctgccgctg gaaattgcag ccgagacgaa gctgcccgag gtttacatcg ccgatttgat
     4681 gcaggcatgg ttgggcattc tttctcgggc aaaacatacg cgcagccacg aagtaatcca
     4741 agaaaccctt tccgtgcgcg cgcaaatgac ggcaatcctg cgccgtttga acgaacacgg
     4801 gatatgcagg tttcacgacc tgttcaatcc cgaacagggc gcggcttacg tgatcgtcaa
     4861 cttcatcgcc ctgttggagc ttgccaaaga aggattggtc ggaatcgtac aggaagacgg
     4921 tttcggagaa atccgaatca gcctcaatca tgagggggcg cattcagacg gcattttcgg
     4981 cacacggggc gggcgcgatg tgttctaata cgccccaagc cgccaccaga aatcgggaga
     5041 cacgccatat gaccggcatc atacattcgc tgctcgacac tgacctctac aaattcacta
     5101 tgctgcaagt agtcctgcac cagtttccgc agacgcacag cctttacgaa ttccgctgcc
     5161 gcaacgtctc gaccgtctat ccgcttgccg acatcaggga agacttggaa gccgaactcg
     5221 acgcgctctg ccggctgcgc ttcacccacg acgaactcgg ctatctgcgc tccctgcgtt
     5281 tcatcaaaag cgactttgtc gattatctcg aactcttcca gctccaacgc cgctttgtcg
     5341 aagtcggcac agacgataaa ggccgtctga acatccgtat cgaaggcccg atgatacagg
     5401 cgatgttttt tgaaatcttc atcctcgcca ttgtcaacga actttacttc cgccggctgg
     5461 aaacccctgc cgttatcgaa gaaggcgaac gccggcttca agccaaagcc gcgcgcctga
     5521 aagaaatcgc cgccgcacag aatccggacg aaccgccctt cctgatttcc gacttcggca
     5581 cgcgccgccg ctacaagctc gcgtggcagg aacacgtcat ccgcaccctg ctcgaagccg
     5641 ccccaagcat cgtgcgcggc acaagcaacg tttacctcgc caaaaaactc ggcatcaccc
     5701 ccatcggcac catggcgcac gaattcctcc aagccttcca agccctcgac gtgcgcctgc
     5761 ggaatttcca aaaggccgcg ctcgaaagct gggtgcacga ataccggggc gatttgggcg
     5821 ttgccctgac cgacgtggtc ggtatggatg ccttcctgcg cgatttcgac ctctatttcg
     5881 ccaaactctt cgacgggctg cgccacgaca gcggcgaccc ttacgtttgg ggcgacaaag
     5941 cctacgccca ctatcaaaag ctcaaaatcg acagccgcac caaaatgctg accttctccg
     6001 acgggctgga catcgaacgc tcttgggcat tgcaccaata tttcaaaggc cgcttcaaaa
     6061 ccggcttcgg catcggcacc aacctcacca acgatatggg gcatacgccc ttgaatatcg
     6121 tgttgaaact ggtcgaatgc aacgggcagt ccgtcgccaa gctctccgac tcgccgggca
     6181 aaaccatgac caacaacagc accttcctcg cctacctgcg ccaagtgttc ggcatacccg
     6241 aacctagaac gccgtaaacc ggcagaaaaa gcgtacaatt cctgtttccg ccgcataaaa
     6301 tcttttaaaa taccgcctga tttgaattta accgaaagac cgaacttcat gaacctacat
     6361 caaaccgtcg aacacgaagc cgccgccgcc tttgccgccg caggcatcgc cggcagcccc
     6421 gttgtcttgc agccgaccaa aaatgccgag cacggcgatt tccaaatcaa cggcgtgatg
     6481 ggggcggcga aaaaagccaa acaaaatccg cgcgaactgg cgcaaaaggt cgccgacgca
     6541 ttggcgggca acgccgtcat cgaaagcgcg gaagtggccg gacccggctt tatcaacctg
     6601 cgcctgcgcc acgaatttct cgctcaaaac attcatgcgg ctttgaacga cgcgcgtttc
     6661 ggcgtagcga aaactgccca accgcaaacc gtcgtcatcg actattcctc gcccaatctg
     6721 gcgaaggaaa tgcacgtcgg ccatctgcgt tccagcatca tcggcgacag tatttcgcgc
     6781 gtgttggaat ttacgggcaa caccgttatc cgtcaaaacc acgtcggcga ctggggtacg
     6841 cagttcggca tgttggtcgc ttatttggtc gagcagcaaa aagacaatgc cgcgttcgag
     6901 ctggcggatt tggagcagtt ttaccgcgcc gccaaagtgc gctttgacga agaccctgcc
     6961 tttgccgaca ccgcacgcga atacgttgtg aagctgcaag gcggcgatga aaccgtgttg
     7021 gcgttgtgga aacagtttgt cgatatttcg ctctcgcacg cccaagccgt ttacgacacg
     7081 ctgggcttga agctgcgccc cgaagatgtg gcgggcgaat cgaaatacaa cgacgatttg
     7141 cagcccgtag ccgatgattt ggttcaaaaa ggcctggcgg ttgaggacga cggcgcgaaa
     7201 gtcgtgttct tggacgagtt caaaaacaaa gagggcgaac ccgccgcatt tatcgtgcaa
     7261 aaacaaggcg gcggcttcct ttatgcctcc accgacttgg cgtgcctgcg ctaccgcata
     7321 ggccgtctga aagccggccg cctgctgtac gtcgtcgacc accgccaagc cctgcacttc
     7381 gagcaactct ttaccacttc ccgcaaagca ggctatctgc ctgaaaatgc aaaagccgag
     7441 tttatcggct tcggcaccat gatgggcaaa gacggcaaac cgttcaaaac gcgcagcggc
     7501 gacaccgtaa aactggttga cctgctgacc gaagccgtcg agcgcgccac cgctttggtg
     7561 aaagaaaaaa atcccgaatt gggcgcggat gaagccgcca aaatcggcaa gaccgtcggc
     7621 atcggcgcag tcaaatacgc cgatttaagc aaaaaccgca ccagcgatta tgtgttcgac
     7681 tgggacgcca tgctctcatt tgaaggcaac accgccccct acctgcaata cgcctacacc
     7741 cgcgtgcaaa gcgtgttccg caaagccgga gaatgggacg caaccgcacc gaccgttttg
     7801 accgaaccac tggaaaaaca gcttgccgcc gagcttctga aatttgaaaa cgtgctgcaa
     7861 agcgtggcgg acaccgcgta tccgcactac ctcgccgcct acctttacca agccgccacc
     7921 ctgttcagcc gcttctacga agcttgcccg attttgaaag ccgaaggcgc aagccgcaac
     7981 agccgcctgc aactggcaaa actcaccggc aacacgctga aacaaggctt ggatttgctg
     8041 ggcatcgatg tgttggacgt aatgtaaaac cgcaccgccc gattgcggac aacagccctg
     8101 caatcaaccg gcggaccatc ctcatccgaa tctgaaaaag cggcgcgata caccgtatcc
     8161 gccgcccctc ccaaaatgcg aaacagacaa acgccaagca agcaagcaag caagcaagca
     8221 agcaagcaag caagcaagca agcaagcaaa aaattatacc cccccttcct gccgacgcac
     8281 gcactttccg cgcggcgtat tccccttttc ccgcccctca aacccgcctt ttcttcaggc
     8341 agggtttcag cccgcctcct ttccctgttt tccttccctc aacacgcgtg ctcgccccct
     8401 gccgcaccgt gccgcacttt cgcgcccgga cggcatcgtt ccgccatccg gttctctgtt
     8461 tcaaataccc ctgtttcaga aagaaatgca gatgtttcaa cacacaggac gacatataaa
     8521 gtaccgccct atgtgtcgtc ctgatttgga aggggttgcg ccccttccta aatatagtct
     8581 gatcctgccg ccccgaagga cagatgtccg agtggcgggg tttcaaccga aaaggaaata
     8641 cgatgaatat tcacgccctg ctctccgaac aatggacgct gccgccattc ctgccgaaac
     8701 ggctgctgct gtccctgctg atactgctgg cccccaatgc ggtgttttgg gttttggcac
     8761 tgctgaccgc caccgcccgc ccgattgtca atttggacta ccttcccgcc gcgttgctga
     8821 tcgccctgcc ttggcgtttc gtcaaaattg ccggcgtatt ggcgttttgg ccggcggttt
     8881 tgtttgacgg actgatgatg gtcatccagc tcttcccttt tatggacctc atcggcgcca
     8941 tcaacctcgt ccccttcatc ctgaccgccc ccgcccctta tcagataatg accgggctgt
     9001 tgctgctgta tatgctggcg atgccgtttg tgttgcaaaa agccgccgtc aaaaccgact
     9061 tccgacacat tgccgtctgt gccgccgttg tggcggcagc cggctatttc accggccatt
     9121 tgagttacta cgaccggggg cggatggcca atatcttcgg cgcaaacaac ttctattacg
     9181 ccaaaagtca ggcgatgctc tacaccgtca gccagaatgc cgactttatt accgccggcc
     9241 tggtcgaccc cgtcttcctc cccttgggca atcagcagcg tgccgccatg cggctgagtg
     9301 agccgaaatc tcaaaaaatc ctctttatcg tcgccgaatc ttgggggctg ccgggcaatc
     9361 ccgagcttca aaacgccact tttgccaaac tgctggcgca aaaagaccgt ttttcggttt
     9421 gggaaagcgg cagttttccc ttcatcggcg cgacggtcga aggcgaaatg cgcgaattgt
     9481 gcgcctacgg cggtttgcgc gggttcgcac tgcgccgcgc gcccgacgaa aaatttgccc
     9541 gctgcctccc caaccgtttg aaacaagaag gttacgccac ctttgcgatg cacggcgcgg
     9601 gtagttcgct ttacgaccgc ttcagctggt atccgagggc gggctttcaa aaaatcaaaa
     9661 ccgccgaaaa cctgatcggt aaaaaaacct gcgccatttt cggcggcgtg tgcgacagcg
     9721 agctgttcgg cgaagtgtcg gcatttttca aaaaacacga caagggactg ttttactgga
     9781 tgacgctgac cagccacgcc gactatcccg aatccgacat tttcaaccac aggctcaaat
     9841 gcaccgaata cggcctgccc gccgaaaccg acctctgccg caatttcagc ctgcacaccc
     9901 aattcttcga ccaactggcg gatttgatcc gacgccccga aatgaaaggc acggaagtca
     9961 tcatcgtcgg cgaccatccg ccgcccgtcg gcaacctcaa tgaaaccttc cgctacctca
    10021 aacagggaca cgtcgcctgg ctgcacttca aaatcaaata acaacaatgc cgtctgaacg
    10081 caccaacagc cttcagacgg cattttgcag acagaccgac ctcctcaagc ccactttttt
    10141 catcatctcc gataaattgc tttgtaagaa tccaaaaatc caaaatcaca ggaagttatc
    10201 aaaaaaacag aaaccatccg ccgtcgttcc cgcgaaagcg ggaatccaga cccggaaacc
    10261 gcagaaattt atcggaaaaa ccgaaactcg aaaacaacgg acaaaattcc cgcctgcgca
    10321 ggaatgacgg ctcatacatc acccaaaaaa aattgaaacc aaacagctgg gattccctct
    10381 tgcgtgaggc tgacagatgc cgtctgaaag actttcagac ggcatagggc gttttctctt
    10441 tgaatttaaa ttttgccaca aaaaagccgc cccaaatgaa tacccgggca gctttttgtt
    10501 gatatgactc caatcagcgg tgttgcggat tgtaacgttt ttccaaacgc aggaatatcc
    10561 agcctaagaa agtcgtcatc aacagataaa tcagggcggc ggtgtaaagc ggttcttcat
    10621 aaaccgaata ccggcccgta atcgtattct gaacatacgc caactccgcc acagcaatga
    10681 ccgacagcag cgagctgtct ttcaagagcg tgatgaactc gctcgccaaa ggcggcagca
    10741 tacggcgcaa tgcctgcggc agaatcacat agcgcatcgc ctgcggatag gtcagtccca
    10801 aagaacacgc cgcctccatc tgtcctttgt ctatagactg gatgcccgcg cggaaaatct
    10861 cacagatata cgcccccgag ttggcgatca gtgccaaaga accggcaatc agcggcccgt
    10921 atccgcgacg cagcgcgatt gccgcctcgc cgctgaccaa aatgccgtct gaaggatgga
    10981 cgaaaaacgg aaaccacaca tacgcccaaa tcacaatctg cacaaacagc ggcgtacccc
    11041 ggaacagggt aacgtacagc agcgaaacct tacgcaacgc ccacgccagc acgcgcatcg
    11101 gcgcaccggc tttttccaag tgaatcaggc gcgccaacgc caacaacaga cccaataccg
    11161 aaccgcccgc cgtcgccacg accgtcagcc ccaaggtcgt cagtgcgccg taaagaaaca
    11221 tccagcggta ttcgtaaata atgtcaaaac gaaaatccat aaaccgtccg tatcaaaaac
    11281 cggcggaact gccgccgttg caaaataatc cgccatttta ccgtaaaaac cgccgcctga
    11341 acttttttat cgcggcagac ggcggttgcg cgtctccgca aaaatgcagg gcgcgcggtt
    11401 ttcagacggc atttgccgtt caaggccgtg cggtgtcttt accaaatgcc caaccattcg
    11461 cccacggaat ccatccaatc cttattgccc ccgccgctcc tgcctgcccg gcggtacgcc
    11521 cacggcgctt gcggattttt agctttccac aatcctttgc gttccctttc cgcctgaatt
    11581 tgagcgtcgg catagtcggc aaaatccgcc ttatcctgct gttctttagc ataactttta
    11641 taatgccacg ccgccccgtc ctgcacctgc atcaggttca aatcggtttt gccggcggat
    11701 acctgcgcca cttcgcgctg atagcggtcg gtttcaaaca cacgtacact gactttccta
    11761 ccctccgccg ccgcgcgcag gttgtcgcgc gaacgtgtac cgtaagcctg tttcatctcc
    11821 ggtgcgtcga tatacgccat ccgaatttta tgtttcgcgc cgtcgccgtc gatgacgtga
    11881 agggtatcgc cgtcatagac tttggacacc gtgcctgtgt agctgtggcc ggatttcgcc
    11941 gatgcccgtc ggcgaacggg cgcgtcggaa cccacgtccc ctgccgtgcc gagtacgtcg
    12001 agtacggcaa ccgccgtccg caccgcctca ctgtcatatc ccgtataacc caacgcgccc
    12061 aaaagcgaca gggcgacggg aagccatttc atgatttttt taatctgcat atttttcaaa
    12121 tgccgatgcc gtctgaacat ctctgaaccg gatttcagac ggcatcttaa cgtcaggatt
    12181 acccttggca gggatagatg actttcgcac cctcttccgt ccccaaaatc aacacatcgg
    12241 cggcatcgcg ggcgaatatg ccgttttcga gtacgccggt gattttgttg atttcatctt
    12301 ccatcgtcag cggctgatcg atattcaagc cgtggacatc gacgatttgg ttgccgtaaa
    12361 acgtggtgta gccgatacgc agttcgggct gtccgcccat ggcaagcagt ttgcgcgaaa
    12421 caagggagcg cgcgctttcg acggcttcca cgggcagtgg gaatttgccc agccgggaaa
    12481 catatttgct ttcgtccgca atgcagacga atttttctga tgcgctggcg acgatttttt
    12541 cgttgaggtg cgcgccgccg ccgcccttaa tcatttgcag ggcgtggttc acttcgtccg
    12601 caccgtcgat atagaccgcc aaaccggaca cttcgttcag ggaaacgacg ggaatgtcgt
    12661 accgggcaag cagttcgccg gattttttgg aggttgaaac cgcgcctttg atttttttac
    12721 cgcttttgcc caaggcttcg atgaagaaat tgatggtcga accggtaccg atgccgatgt
    12781 attcgttttc gggtacgaat tcgactgctt tttcggcggc gatgcgcttg agttcgtctt
    12841 gtgtcgtcat atttttatcc tttgggaaac cgtatcaaca aacagccgcc atcttaacat
    12901 ttttttgcac gtcctgcccg ccgcgttcaa atccgtacca gcaataccgc cgcctgcgcc
    12961 tctatgcctt ccatccgccc gagatagccg agtttttcgt tggttttgcc tttgatattg
    13021 acgcacgaaa tatcgatacc caaatcggcg gcgatgttgg cacgcatttg cggaatgtgc
    13081 ggtgcgagtt tgggtttctg tgcaatcacg gtcgtatcga cattgaccgc ctgccaaccc
    13141 tgcgcctgaa cgctttgata cgccgcacgc aaaaggacgc ggctgtccgc atctttgaac
    13201 tctgcggcgg tgtcgggaaa atggctgccg atgtcgccca aacctgccgc accgagcagc
    13261 gcgtcggtaa cggcgtgcag cagcgcgtcg gcatcggaat gcccgagcag ccctttttca
    13321 aatgggattt caactccgcc aagtatcagc tttctgcctt cgatcaattg gtggacatcg
    13381 tagccctgtc cgatacggat attcgtcatc gtttgtgttc ctgatgtttt gaattgaatt
    13441 tcagacggca ttgagcagca gcctgacgat gtatgcgtcc tgcggctgcg tcagtttcaa
    13501 attgcgcgcg tcgccctgta tcagtagcgg acgcacaccc agtttttcca cggcggacgc
    13561 ttcatcggta atgccgtcca agttttccgc agccaatgcg cggtgcagca aacccgcttg
    13621 aaaaagctgc ggcgtttgcg cctgccaaag ccccgaacgg tcgacagttg cactgatttg
    13681 tccgctttct gcgcgcttga gcgtatcggc aacgggaact gccaaaatcc cgccttcggc
    13741 ggcgttgccc gcctgttcta tcaaccgcgt cagagcttca gacggcaggc agcagcgggc
    13801 ggcatcgtgt accagaatat tgtcggtttc cgccgccaaa ccggtttcca acagttttgc
    13861 cacaccgttg cggacagttt cggcgcgggt ctgtccaccg tttttccaca cccgaacctg
    13921 tggaaatgcc gtctgaacct tatcggcaaa cgtgtcttcg ggcgagacga caacgacggt
    13981 caaatcgacg gcctcatgcc gttcaaaaat cccaagtaca tgttctaaaa cggttttgct
    14041 tccgatttcg acatattgct tgggtttgtc cgcaccgaaa cgcaccccga tgccggcggc
    14101 gggaatcagc gcgatatttt tgcgtttcat gcgttttccc cgccgttttc agacggcacg
    14161 gcttccttac gccaaataca ggcttcgccc aagccgtcaa gatattcttc gtgcgccgcc
    14221 agttcggctt catccgcctt aatcactttc agtttggcac cgagtttcat ttcggcagat
    14281 gcaacaggtt tggctgtcgg ttcttcctcc tcggtctccc ccatcaagtc gaattgccgc
    14341 cgcgtcatcg ccagatagac ttcgcccaaa agttcgcagt cgatcaatgc gccgtgcagg
    14401 acgcgcttgc tgcggtcgac ggaaaaacgg ttgcacaggg catccagact ggctttctgc
    14461 ccggggaaca tttcgcgcgc catcgccagg gtatcggtaa cggtgcagcc gagttcctcg
    14521 acggtcggca gccccatccg gcggaactcc atattgagga agcccacatc gaatttggca
    14581 ttgtggataa tcagttccgc accgcgcaga aaatcggcaa tctgcctgcc gacctctgca
    14641 aacggcggcg cgtttttctc ttccaaaacc tgtatcgtca agccgtggac gcgtgccgcc
    14701 tcttcgggca tatcgcgctc ggggtggaca tagaggtgca ggtttttgtc ggtcatttgg
    14761 cggttgacca tttccagacc ggcaaactcg accagacggt cgccgccgtc ggcatacagg
    14821 ccggtggttt cggtatcgag gatgatttgg cgtgtcatca tatcggtgtc tttcttctgt
    14881 cttcgtaaat tgattatttt ttaagcaatg tatttttctg ttttcatttc aatgcacaaa
    14941 cccacttatt cacagtgtgt tcacaacatt gggcaggcgg attgtgtatt ttggggacaa
    15001 ttttttcaga cggcattcaa ggttttttcc tgattgccgc cgcgcctaaa aaccgccttt
    15061 cgcgcttaat caaaaatacc gccaacggaa tattgcccaa ggcgacaatc agatacagca
    15121 gggaaatgct gtcaaacaaa aacagcaata ccgcgctcaa aacggcggcg gaaaccataa
    15181 agatgccgtt aacgatattg ttggcggcaa cggcgcgggc gcggaaagtc tcgctgctgg
    15241 cggtttgcag ccaggtatag agcggaacgg agaaaaatcc gccgaaaaag ccgatcagcg
    15301 tcatcaccgc catcacggag tatgcccatc cttgcgataa aaaccaaaaa atgccgttca
    15361 gcccttcaaa acggtgtccg tgcgtcagcc acaccaaaac caggccgcaa accgtcaaac
    15421 ccaacgcacc aaccgttacc caagccaaca tcagccgttc cctgccgaac ttggcacaca
    15481 gtaccgaacc ggcggcaata ccgatggaaa acaaagcaag catcaggtta aaaacattat
    15541 cgttgccgcc caaatggatt tgggtaaagg tcggcagttg cgtggtataa accgcgccga
    15601 caaaccaaaa ccacgagatg ccgataatgg cggtaaaaac gggattgtgc cgcaccgttt
    15661 cacgcagcag ggattttgta ccacggacaa tattccactc gatttgggta tcggcagcct
    15721 tggcgggtac ggacggcata aacaggctgc cggccgttcc tccgacggcg accagcaaaa
    15781 ccagtatccc gacaatataa ggcggcgcgc cggcaaccgc cgttcccaaa atctgaccaa
    15841 acaggatggc gacaaacgta ccggattcaa tcaggctgtt gcccatcatc agctcgttgt
    15901 cgtcgagata atcgggcaga atggcatatt taagcgggcc gaacagcgtc gattgcgcgc
    15961 ccatgcaaaa caggcagagc aagagcagcg gggctgaccg gatataaaac ccgtatgccg
    16021 ccaccgccat aatgatgatt tccagcacct tgacccaacg cgccaaaacg gccttgtcga
    16081 atttgttacc caactgcccc gacagcgcgg aaaacaggaa atacggcaaa ataaacagca
    16141 gtgcgcccaa gttcaacatc tgtccggcag gcagaacgcc gttttgcccc aaaccgtaaa
    16201 acccgatcat cacaaacagc gcggttttga acacattgtc attgaacgcg ccgagaaact
    16261 gcgtagcgaa aagaggcccg aaacggcggc ttttaaccag tcccaaaccg ccttttttag
    16321 cgtccatcgt tttccttctc tttttcaatc agtttacttg tcgaatcatc atccatcaaa
    16381 atgcggtgcg ccggtccttc caaatcgtca aactgtccgt ttttgcccga ccaccagaaa
    16441 aaccagccga tgacaaatgc caaaataatg ctgatgggca ccagaataaa catactttcc
    16501 atcacatatt ccctatcaaa tcgttcaaaa caaaagtctg ccctgacacg gtcagatatt
    16561 cgttacgcaa agttccgacg ggggcttcgt caaaaaacag ctcgatacgg tctttgacca
    16621 cgcgccaata ttggggaatt tccgtctgac cgaacggcga caggacatgg ttgcccgtcc
    16681 catcttccaa tttaacggca aaacgcccgc tctgcagccg tgcttccgat tcgtcgtcgg
    16741 caagaaggat gaaaaagcct atatgccgtc ccgattggtc atgaatactg aaataatgca
    16801 taaattcccc acccgccttt tttcagacgg catcaactaa aaacagggcg aatgtaccag
    16861 tttggacggg aaaaatgcaa agaaattctc cctcccccag ccgaaaacac cggcaaaccg
    16921 catatccccc ttttttccgt caaaataccc gacttccgcc attttcacgc aaacgcccga
    16981 ttaagccaag caattgcaaa gattttttgc tagaatagcc tgcttctttt atcaaccgtt
    17041 tcagacggct ccactacttt cccgtcaagg aaagcaaagc ggattcggca cgaatccgat
    17101 tagtatccgt gtccgattcc aatgccgtct gaaactttcc ggagtaagaa aatgtcccaa
    17161 aaattgatct tggttttgaa ctgcggcagc tcgtccctca aaggcgcggt cctggataac
    17221 ggcagcggcg aagtcctgct gagctgcctt gccgaaaaac tcaacctgcc cgatgcctac
    17281 attacattca aagtaaacgg cgaaaaacac aaagtcgatc tgtccgcaca ccccgaccac
    17341 accggcgcgg tcgaagccct gatggaagaa ctcgaagccc acggcctcga cagccgcatc
    17401 ggcgccatcg gccaccgcgt cgtcagcggc ggcgaactgt acaacgaatc catcctcgtt
    17461 gacgacgaag tcatcgccgg catcgaaaaa tgcatcccgc tggctcctct gcacaatccc
    17521 gcccacctct tgggcctgcg tgccgcgcaa agcattttca aaggcctgcc caacgtcgtc
    17581 gtattcgata cctccttcca ccaaaccatg cccgaagtcg cctacaaata cgccgttccg
    17641 caggagttgt atgaaaaata cggcctgcgc cgctacggcg cgcacggtac cagctaccgc
    17701 ttcgttgccg acgaaaccgc gcacttcctc ggcaaagaca aaaaagacct gcgtatggtc
    17761 attgcccact tgggcaacgg cgcatccatc accgccgtcg ccaacggaga atcgcgcgac
    17821 accagtatgg gcctgacccc gctggaaggg ctggtaatgg gtacgcgcag cggcgacatc
    17881 gatccttccg tattcggctt cctcgccgaa aacgccaata tgaccattgc ccaaatcacc
    17941 gacatgctga acaaaaaatc cggcctgctc ggcatttccg gcctgtccaa cgactgccgc
    18001 accattgaag aagaagccgc caaggggcat aaaggcgcga aattggcctt ggatatgttt
    18061 atctaccgcc ttgccaaata catcggcagt atggcggttg ccgcaggcgg cttggacgca
    18121 ctggtcttta ccggcggtat cggcgaaaac tccgacatca tccgcgaacg cgtaatcggc
    18181 tacttgggct tcctcggtct gaacatcgac caagaagcca acctgaaagc ccgcttcggc
    18241 aacgccggcg tgattaccac tgccgacagc aaagccgttg ccgtggtcat tccgaccaac
    18301 gaagagctga tgattgccca cgataccgcc cgtttgagcg gtctgtaagg ttttatcctc
    18361 acgcgaactg cctccggaaa cggaggcagt ttttttatag tggattaaat ttaaacc
//
LOCUS       DAXDSD010000039        18407 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXDSD010000039 DAXDSD010000000
VERSION     DAXDSD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18407
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.039"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            42..115
                     /locus_tag="ACFB2Q_001027"
     tRNA            42..115
                     /locus_tag="ACFB2Q_001027"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Cys,seq:gca)
     gene            216..305
                     /locus_tag="ACFB2Q_001028"
     tRNA            216..305
                     /locus_tag="ACFB2Q_001028"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:250..252,aa:Leu,seq:taa)
     gene            409..1461
                     /gene="sohB"
                     /locus_tag="ACFB2Q_001029"
     CDS             409..1461
                     /gene="sohB"
                     /locus_tag="ACFB2Q_001029"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC0918070.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(1512..2366)
                     /locus_tag="ACFB2Q_001030"
     CDS             complement(1512..2366)
                     /locus_tag="ACFB2Q_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFC0918071.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFDERQLYRHYGLVSRLRVLISNREAFGYLCVGAAMG
                     ILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRIEWS
                     ERNIKAGT"
     gene            complement(2377..2808)
                     /locus_tag="ACFB2Q_001031"
     CDS             complement(2377..2808)
                     /locus_tag="ACFB2Q_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFC0918072.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(3043..3912)
                     /locus_tag="ACFB2Q_001032"
     CDS             complement(3043..3912)
                     /locus_tag="ACFB2Q_001032"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC0918073.1"
                     /translation="MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGSTAELVRACPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(3988..4899)
                     /gene="hisB"
                     /locus_tag="ACFB2Q_001033"
     CDS             complement(3988..4899)
                     /gene="hisB"
                     /locus_tag="ACFB2Q_001033"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC0918074.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(4916..5995)
                     /gene="hisC"
                     /locus_tag="ACFB2Q_001034"
     CDS             complement(4916..5995)
                     /gene="hisC"
                     /locus_tag="ACFB2Q_001034"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC0918075.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFIMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(6041..7330)
                     /gene="hisD"
                     /locus_tag="ACFB2Q_001035"
     CDS             complement(6041..7330)
                     /gene="hisD"
                     /locus_tag="ACFB2Q_001035"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC0918076.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     ATKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(7327..8214)
                     /locus_tag="ACFB2Q_001036"
     CDS             complement(7327..8214)
                     /locus_tag="ACFB2Q_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC0918077.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(8306..8971)
                     /gene="hisG"
                     /locus_tag="ACFB2Q_001037"
     CDS             complement(8306..8971)
                     /gene="hisG"
                     /locus_tag="ACFB2Q_001037"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC0918078.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(9050..9703)
                     /locus_tag="ACFB2Q_001038"
     CDS             complement(9050..9703)
                     /locus_tag="ACFB2Q_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC0918079.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            9979..10063
                     /locus_tag="ACFB2Q_001039"
     tRNA            9979..10063
                     /locus_tag="ACFB2Q_001039"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10013..10015,aa:Leu,seq:caa)
     gene            complement(10126..10233)
                     /locus_tag="ACFB2Q_001040"
                     /pseudo
     CDS             complement(10126..10233)
                     /locus_tag="ACFB2Q_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            10757..12484
                     /gene="ilvB"
                     /locus_tag="ACFB2Q_001041"
     CDS             10757..12484
                     /gene="ilvB"
                     /locus_tag="ACFB2Q_001041"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC0918080.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
     gene            12495..12986
                     /gene="ilvN"
                     /locus_tag="ACFB2Q_001042"
     CDS             12495..12986
                     /gene="ilvN"
                     /locus_tag="ACFB2Q_001042"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC0918081.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            13053..13346
                     /locus_tag="ACFB2Q_001043"
     CDS             13053..13346
                     /locus_tag="ACFB2Q_001043"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC0918082.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            13426..14439
                     /gene="ilvC"
                     /locus_tag="ACFB2Q_001044"
     CDS             13426..14439
                     /gene="ilvC"
                     /locus_tag="ACFB2Q_001044"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC0918083.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(14558..15553)
                     /locus_tag="ACFB2Q_001045"
     CDS             complement(14558..15553)
                     /locus_tag="ACFB2Q_001045"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC0918084.1"
                     /translation="MNLKNRHFLKLLDFTPEEITTYLDLAAELKDAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGADATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFAQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(15695..18280)
                     /gene="acnB"
                     /locus_tag="ACFB2Q_001046"
     CDS             complement(15695..18280)
                     /gene="acnB"
                     /locus_tag="ACFB2Q_001046"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC0918085.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
BASE COUNT         4120 a         4482 c         5130 g         4675 t
ORIGIN      
        1 ctcgtttccc gctccaagtt ttgtagacaa gccagtttta gggcgagata gcaaagtggt
       61 tatgcagcgg attgcaaatc cgtctacgcc ggttcgattc cgactctcgc ctccatatat
      121 cttacggcgg ggtggcagag tgtttatgct atgaggggtg caaccttcat ataggccggt
      181 taaaatccgc gcccccgcct ccacctttca caaatgcccg ggtggtgaaa taggtagaca
      241 caacggactt aaaatccgtc gggactaaac atcccatgcc ggttcgattc cggctccggg
      301 caccaagctg aaaatgacaa tgccgctcca agcggttatt tttttatctg tcggatgggg
      361 atggacaata ttgatatgtt catgcctgaa caagaggaaa tccaatcaat gtggaaagaa
      421 attttactga attacggtat tttcctgctc gaactgctta ccgtgttcgg cgcaattgcg
      481 ctgattgtgt tggctatcgt acagagtaag aaacagtcgg aaagcggcag tgtcgtactg
      541 acagattttt cggaaaatta taaaaaacag cggcaatcgt ttgaaacatt ctttttaagc
      601 gaggaagaga caaaacatca ggaaaaaaaa gaaaagaaaa aggaaaaggc ggaagccaaa
      661 gcagagaaaa agcgtttgaa ggagggcggg gagaaatctg ccgaaacgca aaaatcccgc
      721 ctttttgtgt tggattttga cggcgatttg tatgcacacg ccgtagaatc cttgcgtcat
      781 gagattacgg cggtgctttt gattgccaag cctgaagatg aggttctgct cagattggaa
      841 agtccgggcg gcgtggttca cggttacggt ttggcggctt cgcagcttag gcgtttgcgc
      901 gaacgcaata ttccgctgac cgtcgccgtc gataaggtcg cggcaagcgg cggctatatg
      961 atggcgtgtg tggcggataa aattgtttcc gctccgtttg cgattatcgg ttcggtgggt
     1021 gtggtggcgg aagtgccgaa tatccaccgc ctgttgaaaa aacatgatat tgatgtggat
     1081 gtgatgacgg cgggcgaatt taagcgcacg gttactttta tgggtgaaaa tacggaaaag
     1141 ggcaaacaga aattccggca ggaactggag gaaacgcatc agttgttcaa gcagtttgtc
     1201 agtgaaaacc gccccgggtt ggatattgaa aaaatagcga cgggcgagca ttggttcggc
     1261 cggcaggcgt tggcgttgaa cttgattgac gagatttcga ccagtgatga tttgttgttg
     1321 aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc aggagaagcg aagcctgatc
     1381 cagcgcattg gtttgcaggc ggaagcttcc gttgaaaagt tgtttgccaa acttgtcaac
     1441 cggcgagcgg atgtgatgta gtttgccgaa cggatggaaa tgccgtctga agtgtgttca
     1501 gacggcattt ttcaagttcc ggctttgatg ttccgttccg accactctat ccgttgtccg
     1561 atgtctttca aattggaata ttgttcgacc aatcgcggca catcgtccaa actcatggca
     1621 aacatccaca gataagtgcc gaccgaataa atatgccccg cgctgccgta gcctttgagc
     1681 gtcatcatca caaaagcaaa gccgaacaaa atacccatcg ccgcgccgac gcagagatag
     1741 ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg aaaccagtcc gtaatggcgg
     1801 tacagctgcc gctcgtcgaa gtggttgtcg cgttccaagc tgttgttcag gcggaaatac
     1861 aggttttcgc tgatggcggc aaaacgtggc aaaagccata aaaacaacgc aagtatgccc
     1921 accgccgaca cgccgaccca aaattccagc accagcagca tgatgcacgc gccgaatatg
     1981 gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa aaaagctgac aaattcacgc
     2041 gacagggcaa cccgtgcagt taccgctgaa tgcgggactt gccgctgccg ttgttccaac
     2101 acaaccggca cggcgatttc ggtataaatc cgcgtaaacg tgcgcgtatc ggcaatccgc
     2161 cgtgccgcac cgaccagcca catcaaaaat acaaccaaag cgtacagcaa cgcctgccac
     2221 accctccccg caatcacggc attaatcgcc cagccgccaa acaccggata ccccagcatc
     2281 aaaaggtttt ccagtccgac cggggaaaat gtgccaatca atcgcttgcg gtgggttttg
     2341 gctatgtgtt tcaacatttt ccacatattt gtccctctat tttttatttt ccgagattgt
     2401 tttttccatc acttccgcca gtgcatccaa atcggcaaac agccgggttg tgcgcttgtc
     2461 gccgaatgtg gaaaatactt tgtcgctgaa ttcctgcgcg ttttctgtta aaggtgcggc
     2521 atggactttg cctttttcgg tcagcgacag caaccgttcg cgcctgtcct gttcgccttc
     2581 ctgccattca atcaaccctt gtccggcaag ggttttgcat acgcctgaaa cggtctgttt
     2641 gggcagactc cactcttctc cgatgtgctt ttgcgtgcgg ctgccttcgg ttgccagggt
     2701 ataaagtacg gtaaagaggt tgtaattcaa atcctgctgc ccgatccatt tgtcaaagac
     2761 attgcaaatc aggttgatac ggattccaag ttggtcgagt cggttcataa ttggtcttga
     2821 tattgactaa atttgtgcgg attatagtgg attaacaaaa accggtacgg cgttgcctcg
     2881 ccttgtcgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
     2941 cactatatag tcataatcgg gactgatttc aagcgtgggg cgataaaaat gccgtctgaa
     3001 acgctttctg gcttcagacg gcatattgtt tggaaaggga agtcagtgtt ctgccaattt
     3061 caggtaaacg ccggaaacgt cctgttcgcc gtagccggct tcaactgctt tgcggtagct
     3121 ggcagcaacg gtttcgacgg cgggcagggt gttgcctgcc tgttcaagct ctttgacggc
     3181 gaggttaagg tctttggaag cgtgtttgag tgcaaaggca ggggggaact cacggtttgc
     3241 ccatagtgat ttttttgttt gaaacatagg cgagtccatt gccgagccgc cgatggcttc
     3301 gacgatggtg tcggtatcga tgccgaactg ccgcgccatc agcatcgctt cgctgtacgc
     3361 ttcgccgaaa atgcctaaga gcgagttcaa gacgagtttc gcgcccgagc ctttgccgac
     3421 atcgccgaaa tggaaggttt ttttgccgac aagggaaaat attttttgca gcgggtttaa
     3481 aacggcttcg ctgccgccga acagaatcag cagtgtgccg ttggtggcgg gtccgaccga
     3541 tccggaaacg ggtgcttcgg caaactgtcc gcctgcggct tcgacaagtg ctttgacggc
     3601 gaggttttcg gtcggggaga tggtgctcat gttgacgatg attttgccgg ccaatccgtc
     3661 gcggactccg ttcaggatgt cgcacacggc ggcatagtcg gaaaccatca ggaaaatgac
     3721 gggacaggcg cggacgagtt cggcggtgct gccgtaaact tttgctcctt tggcggagat
     3781 gggggcagtt ttgtcgggcg agcggttgta tacgccgact tcgatgccgc cgtccaagag
     3841 ccgcgttacc ataggcagac ccatttgccc taagcctacc cagccgattt gtgtgtatgt
     3901 ttctgcggac atagtgtttc tcctttgttg gggcgctgta atgccgtctg aaggcttcct
     3961 gcgcttcaga cggtctgttt ggggatgtta tgcggtcagc gtgcctttgg tcgagggggt
     4021 ttgcccagcc attctcgggt cgtgttcgac tgccatacgc agggcgcgcc cgaaggcttt
     4081 gaatacggtt tccgcctgat ggtgggcgtt tttgccgctg aggttgtcga tgtgcagggt
     4141 catcatgctg tggttgacga tgccgtggaa aaattcttca aacaaatcga catcgaaacg
     4201 tccgattagt gcgcgggtaa attcgatgtt gtacacgagt ccggggcggc cggaaaggtc
     4261 gatgacgacg cggctgaggg cttcgtcgag cgggacgtag gaatgtccgt aacggcggat
     4321 gccttttttg tcgccgagtg cctgccggat tgcttgtccg agtgtgatgc cgatgtcttc
     4381 ggcggtgtgg tggtcgtcga tgtgcaggtc gcctttgcag ctgatgtcga tgtcaatcat
     4441 gccgtggcgg gcgatttggt cgatcatgtg ttcgaggaag ggaacgccgg tatccagcct
     4501 gcttttgccg ctgccgtcga ggttgatgga gacggtgatt tgggtttcgc aggtattgcg
     4561 gttgacggta acgctgcggc agccggcagc agatgcggtt tcggcaattc gggtttcggc
     4621 agttccggtg ctttctgcgg cggtttcggg gacagtgcgt tcgcggtgtt ttctgtcgag
     4681 ccagcctttg ggtttgccgg tgtgtttttc gagttttgcc atcaggctgg gacggatgcc
     4741 gcgcgcatcg gggtcttctg cctgcttttc aaggcgttgt ttgagtttgt aaagtgcgac
     4801 gggggtacgg tagccgcaga gtttggcaag cttgggcagc gaacctgctt cttgggcgag
     4861 agtcaggaag ttgttcaggt ggagttgtgt cttggtcata gggattccgt ttggtttatc
     4921 ggtagagccg gcggataacg tcgaggacgg catcgttttg tgcggagctg cctatggtaa
     4981 tgcgcaggca gtgttccaaa agcggatgtg cgccgtgcag ttttttgacg aggatgcggt
     5041 tttgtttgag tgtgtcaaac aacaaatcgg catcgggtac gcgtatggta atgaagtttg
     5101 cctgacttgg aaaggcgttc agacggcata ttttgcccaa ttcggcgaac atccgttcgc
     5161 gttcgttttt caggctgtcg atgttggcag aaatgatgcc gtagtgttgc agggcgagtt
     5221 tggcggtggt caggctcagt tggttcatat tgtagggcgg caggattttt tgcagttcgc
     5281 cgatgacttc gggacagcct gtcgcataac cgatacgcag tccggcaaaa ccgattttgc
     5341 tgagggtgcg taagactatc aggttgggaa tcctgcccgc ctgcggcagg aagctgtcgc
     5401 cgttgaatgc gccgtaggct tcgtcgacga cgacgatgcc gtctgaagct tcgatggcgg
     5461 cttcgatttc ggcacgcgtg aaacatacgc cggtcgggtt gttcgggtag gcgataaagg
     5521 tcagggcagg gcggtgcttc ctgacggctt cgaggacggc gggcaggttg agggtgaaat
     5581 ctccgttcag tggaacgccg acataatcca tgccgtacag cgcggcgttg tggcggtaca
     5641 tgatgaaacc gggttcggct gccaacattg ccgcgccggg tttggcggtc agcatggtga
     5701 tgaactgtat cagttcgtcc gaaccgttgc ctaaggcgac ggcggcgcag tcagggatgt
     5761 cgaacgccga acgcaatgct tcctgcaaac cgcagccgga ggggttggga tagaggtgga
     5821 tgggggcgga tgccagttgc gcccgccatt cccgcatcag ggcttcgtgc ccctcaaaag
     5881 gatgggcggg gctttccatc gcatcgagtt tggcaaaacc gggcggaaca tcggtgattt
     5941 tgtatgcgga catggcgagg atgtcgttgc ggatgaagga gcggacggat ttcatcgtgt
     6001 ttccttaatg gttggaatat gcctgtacgc cgtttcggca ttatttcata cggaactctg
     6061 ccgcgcgtgc gtgggcggtc aggctttcgc cgtgtgccag cacgctggcg gtttcgccta
     6121 atttttgcgc gccctgttcc gaaacctgaa tcaggctgga gcgtttttgg aaatcatatg
     6181 tccccaaagg cgaggaaaag cgggcggttc ggctggtggg caacacatgg ttcgggccgg
     6241 cgcagtagtc gccgaggctt tcgccggtgt agcgtcccat gaaaatcgcg ccggcatggc
     6301 ggattttttt cgcccattcc tgcgggtttt cgactgacag ttccaagtgt tcgggggaaa
     6361 tgtagttgga gatttcgcag gcttcgttca agtctttaac gagtatcatc gcgcccctgt
     6421 tgccgagcga ggcctcgatg atgtcgcggc gcggcatggt ttcgatgagc ctgtccatgg
     6481 cggcttctac ttcgtcgaga tacgcttgcg acgtgccgat gaggatggct tgggcaattt
     6541 cgtcgtgttc ggcctggctg aacaaatcca tcgccaccca atcggcgggt gtcgtgccgt
     6601 cggcgatgac cagtatttca gacggccccg ccaccatgtc gatgccgacc acgccgaaca
     6661 cgcggcgttt ggtggcggcg acgaaggcgt tgcccggacc ggtgatttta tcgacttggg
     6721 ggatggtttc cgtgccgtag gcgagggcgg cgatggcttg cgcgccgccg acggtgaaga
     6781 ctttggttac gccggcgacg tatgcggcgg caagcacgat gtcgttgcgt tcgcctttcg
     6841 gtgtcggtac gaccataatg atttctttca cgcctgcgac gtgggcgggc atcgcgttca
     6901 tgatgacgga actcggatat gccgccttgc cgcctgggac gtaaatgccg acgcggtcaa
     6961 gcggggtaat ctgttgcccc agcagcgtgc cgtcttcatc ggtgtagctc cacgattcca
     7021 ttttttggcg ttggtggtag ctttcgacac ggcgggcggc ggtctgcaat gctgtctgaa
     7081 cgtcgttcgg aatgcgctcg aacgccgcgt tcaaatcggc ttgcgtgagt attaaatcat
     7141 cgatgctttt agcgtttgtc tgatcgaatt tgttggtgta ttcaatcaaa gccgcatcgc
     7201 cgcgcttttg cacgtcggcg caaatgtcgg cgacgatgcg ttcggtttcg gggttttgcg
     7261 cggtttcaaa agccagcagg gctttgagtc cggcttggaa atcgggcgat tgggtgttga
     7321 gttttttcat gatttgggat tctcctttgt tattttggga cttgggatcg tctgaagcgt
     7381 ctcttgctcg gttttgggtc tgatatacgc ttttcctccc ccgctaccaa acttcaattc
     7441 gggcaggcat ttttgttgca gaccgagttt ggctaatacc tcttcgctac gagaggtagt
     7501 acggaaacgg taacgggttt gttttctgcc gtttttggtt tcctcagtca ttgtttcaaa
     7561 accgtccact tcgtacgcac tgacaacagc gttttgcaga ccggtgtgaa cgccgataac
     7621 gtatttcact tttgaagcaa catctttacc gataacccag ttgcccaacg tacgcgattt
     7681 gaggttggca tcgtcttggt tgtcgaaaag gtagtctaat tcttcgtcag tatctaaatc
     7741 gaaagcattg tggatcttga tggcgagaat cagcccgtcg gggttaatct cgctaagtgg
     7801 gagagaagag aatccaaagc ggcgatctag ttcttctacg ctaataccac ccggaccatg
     7861 cccggcaatt ttatttttga gttttttacc caagacagat ttaacaaaat gaattaaggc
     7921 agattcggcg gcgagcgctt cgacttcagt cagatgatag ctgatgatat agcgaccgag
     7981 tttcttgcat ttggagatgg ctttgagttt ccgatcgata atctcgccgg agactgggtc
     8041 ttgtgaacga ctagcaaccc attcatgctc gaagatacga ttaccgcaac cttttccgat
     8101 gtagaggatt ttgtcttttt tcaggtcggt cagacaatag acataaaacc gacgctcgcc
     8161 gttattcaaa acggaaaggg tcgaatctga gaattttttt atttttgcaa ccattttaac
     8221 ttctcctata cagtttactg ccggtgatat tttcagacgg cctgtcggcg ggaacggata
     8281 gggtcgtctg aaaacgcatc tttattcaaa tgaatgccca cttcgccttc actgcgccgc
     8341 cgaacgactg aataatcggc tccagcagcg cgtatttcgt tttcaaagca gccttgttga
     8401 ccaccaggta gctggaaatg tcgacgatgt gttcgactgc ctccaagccg tttgccttca
     8461 aggtgtttcc cgtcgaaacc aagtccacaa tcgcatcgct caagccgacc agcggcgcaa
     8521 gttccatcga gccgtacagt ttgataatgt ccacatggac acccttgccg gcaaaatgag
     8581 atgccgcgat ttcaggatac ttcgtggcaa tcttcagacg gcatccgggt tgcgaagcgg
     8641 cttcgtaatc aaacccttta cgcacagcaa ccatcatgcg gcacttggca atctccaaat
     8701 ccaaaggccg gtaaagcccc gtgccgccgt gttcgatcag cacgtctttg cccgcaatgc
     8761 cgaagtccgc cgcgccgtag cggacataag tcggcacatc ggttgcgcgg acaatgacaa
     8821 ggcggatgtt ttcatggttc gtcccgataa tcagcttgcg cgatttttcg ggctcttcag
     8881 tcggagcaat gccggcagcg gcaagcagcg gcagcgtctc ctcaaaaatg cgccctttgg
     8941 ataaggcgat ggtcaaagca ttatcctgca tggtgtggat gtcctttgtc ggttgaaaca
     9001 ataatgccgt ctgaacaggc gccggcggtg cagacggcat acggttttgt cagagcaggg
     9061 tccttacatc ggcagcaatc gtttccggct cgcttccgta aggcgagaaa atggcaacct
     9121 caccgttttt gtcgatgaga tacgcaccgg aagagtggtc gaccaaatag ttttcgctgt
     9181 cgtctttttg attgattttg gcagaaacca cgcggtattg ctgcttgatg accggcaggt
     9241 tttgaccgcc cgttgccgtc agaccgataa agtccggatt gaactgtttg gcatacttgc
     9301 cgatgatttc aggcgtgtcg cgttccggat cgatgctgac gaacaccact ttcacgtcct
     9361 tagcctgccc gcccaactgc ttcaaagtgt cgctgtacgt caaaagctct gtcgggcaga
     9421 catcgggaca gtgcgtaaag ccgaaagaca gaatcacgac cttgcctttc aaatcgctca
     9481 ggctgaaagg cttgccttcg ccgtcggtca gcgtgaaatc gccgccgatg tcttccttac
     9541 gcatatccgt accgcgcgtt tgcggctttg ccgcattttc cgcagccggc gcggatgcac
     9601 ttgaagaagc ggcttgcgcc gcactgttgt cttgaggttt gcaggcggca agcgcggcaa
     9661 gtacgaaaac gcccggcaaa aaggaacgcg gtacggaaaa catgacaatc tcccaagaat
     9721 aaatagaaaa acctgattcg tacggaattt gaatatttcc atcttaacgc gtttggcaga
     9781 tttaatgtag tattttttta cgttgtccgt acggcaggaa ttgccgaatc atacagaatc
     9841 tttggagcgg aaagaacagt aagattaaaa taataaaaat tctatttaaa atcaaaaaca
     9901 aaataaaaac agataaaata atttcaaaaa aaggtttaca gaatctcaaa atccattata
     9961 atggcaattt cttcaccagc ccaggtggcg aaattggtag acgcagggga ctcaaaatcc
    10021 cccgccgcaa ggtgtgtcgg ttcgagtccg accctgggca ccacaaccgc ttttggagcg
    10081 gttatttttt tgtctattgc tgtttaatga aatagataag actaaatata gtggattaaa
    10141 tttaaaccgg tacggcgttg cctcgccttg ccgtaccatc tgtactgtct gcggcttcgt
    10201 cgttttgtcc tgatttaaat ttaatccact atacaatcaa tatatttaag tcgttgttag
    10261 ccctcttggt tctgtcaatg ccgtccggtg caatgagcca ttttcaggaa gtatggttgg
    10321 gaatattttt gatatatcaa aaataaaatc aagttcaatc aatatcccga gtgaaaccct
    10381 gtaaatttta aaattcttga tactttcaca tctgccccca tgcaaactgt caacaatttc
    10441 catctttcaa aaaaggtact cctgatttta ttcttttaaa atcaatcaat aatttctccg
    10501 ctttcaaaaa aatttctccg ctttcaaaaa atattaaaag atgaaagatt tttcttgatt
    10561 gaactgtcgg tttgcgttat cgtttttact ctttttcaca aactctgtgt tcctttccaa
    10621 ttgattggat aatctgtctg ttgccgtgtt cccccaatgc gcgtacccta aatcgctgct
    10681 tggtggaatt gcattacagg tgctgtggca aagcggtttg tccctattgg tttgaaaccg
    10741 tataaaagag gtcgttatgc agttatcagg cgcgcaaatc atagtgcaga gtctcaaagc
    10801 cgaaggcgtc gagtacgttt tcggttatcc cggcggtgcg gttatcgaaa tctacgatgc
    10861 cctgttccaa ctcaataaat tcaaacacat tcttacccgc catgagcagg cggcagtaca
    10921 cgcggcagat gcgtatgcgc gcgtcagcgg taaggtgggc gtggcattgg ttacatccgg
    10981 cccgggcgtt accaatgcgc tgaccggtat tgctactgcc tatacggatt cgattccgat
    11041 ggtggtcatc agcggacagg taggcaattc cctgattggt acggatgcgt tccaagaagt
    11101 tgatacggtg ggtattaccc gtccgtgcgt caaacacaat ttcttggtta cggacatcaa
    11161 tgagttggtg gaaaccatta aaaaggcgtt ccaaattgcc gcaagcggcc gaccggggcc
    11221 cgtggtgctt gatgtcccga aagatgttac gcaggcgatg gcgaaattca gctatcctca
    11281 ggaagacatt tttatccgtt cgtatcaacc ggttgttcaa gggcatatcg gtcagattaa
    11341 aaaggccgtg cagatgttgg catctgccaa acgcccggtc gtctattttg gcggcggcgt
    11401 ggtgttgggt aatgcttctg aagagctgac ccgatttgtc cgaatgacgg gtgctccgtg
    11461 cacgggtacg ctgatgggct tgggcgctta tccttccggc gaccgccaat tcctcggtat
    11521 gctcggtatg cacggtactt acgaggcaaa ccttgccatg cagaatgcgg atgtcgttct
    11581 tgccgtaggt gcgcgttttg acgaccgtgt ggtttccgta ccgtccaaat ttttcgagaa
    11641 ggcgaagaag gttatccata tcgatgtcga tccttccagc atcgccaaac gcgtgaaggc
    11701 ggacattccg attgtcggcg acgtgaaaaa cattttgtcc gagatggttg cgctgtggca
    11761 aaaacaagag tccgtgccgt ctgaagatgc tttgggcaaa tggtggaaaa ccatagagga
    11821 atggcgttcc cgcgattgct tgtggtttga caacggcagc gaaattatca agccccaata
    11881 tgtgattcag aagcttgccg agattaccgg caattcggca atcatcacat cggatgtagg
    11941 gcagcatcaa atgtttgcgg ctcaatatta tcctttcgaa cgtccgcgcc aatggctcaa
    12001 ctccggcggt ttgggtacga tgggcgtggg tctgccttat gcgattggtg caaaacttgc
    12061 cgccccggat caagacgtat tctgtattac cggcgacggc tcgatccaga tgaacatcca
    12121 agagttgtcc acctgtttcc aatatcggat tccggttaac gtcattacgc tgaacaacgg
    12181 ttatctgggt atggtacgcc agtggcagga aatatattac ggcggtcgag agtcggaaac
    12241 ctatttcgat tctttgcccg atttcgtcaa acttgccgag gcatacggcc atatcggcat
    12301 ccgcgtggac aagaaatctg atgtggaagg tgccttgttg gaagcattga accaaaaaga
    12361 caggctggtg tttatcgact tcctgaccga ccagaaacag aatgtgatgc ctatggtcgg
    12421 caacggcaaa ggtttggacg aaatggtact tccgccgcat atgcgtacgg acggaaaggc
    12481 gtaaggagag gcaaatgcga catatcttat ctgttctgat cgaaaacgaa tcaggtgcga
    12541 tgagccgcgt ggtcggtttg ttctctgcac gcgattacaa tatcgattct ttggcggttg
    12601 cgccgaccga agacaaaacc ctgtcacgga tgaccatcgt tacccacggc gacgagcagg
    12661 ttatcgaaca aatcaccaag caactcaata aattgattga agtgattaaa gtggtcgatt
    12721 tgaacgaaag ccgttttgtc gaacgcgaac tgatgttggt aaaagtccgt gccgccggca
    12781 aagaccgcga cgaattttta cgcttgaccg aaatctaccg gggcagcatc atcgacgtaa
    12841 ccgaccgcag ctatacgatt gaaattaccg gctcgaccga caagctggat tcctttttgg
    12901 aaacggtcgg acgcgcccaa attttggaaa ccgtacgtac aggtgcggcc ggcatcggtc
    12961 gcggtgagcg tattttgaaa atttaacgcc gtaacctttc agacggcatg gtatttgaat
    13021 gccgtctgaa aaacgaacgg caggagagat ttatgtcgaa cattaaaatt gtcgcactgg
    13081 ttaccgtcaa accggaatac acggaaacac tggcagcaca gtttaaagaa ctggtcaaag
    13141 ccagccgtgc ggaagagggc aacatcagct acaatctcca tcaggaaatc ggcaaaccga
    13201 accgttttgt tttcgtggaa aattggaaat cccaagcagc tattgacgaa cataatgcca
    13261 gcgcgcattt ccaagccttc gtccagtccg tcgacggcaa aaccgaagcg ttggaaatcg
    13321 tattgatgaa tgaagttgcc gtttaagcgg cacactctgt ttaacccgtc cgaagccgct
    13381 caacatttta aggcttcgac aaccatttac cttaaaggaa atcaaatgca agtctattac
    13441 gataaagatg ccgatctgtc cctgatcaaa ggcaaaaccg ttgccatcat cggttacggt
    13501 tcgcaaggtc atgcccatgc tgccaacctg aaagattcgg gtgtaaacgt ggtgatcggt
    13561 ctgcgccacg gctcttcttg gaaaaaagcc gaagcagccg gccatgttgt taaaaccgtt
    13621 gctgaagcga ccaaagaagc cgatgtcgtt atgctgctgc tgcctgacga aaccatgcct
    13681 gccgtctatc acgccgaagt tgcagccaat ttgaaagaag gcgcgacgct ggcatttgca
    13741 cacggcttca acgtgcacta caaccaaatc gttccgcgtg ccgacttgga cgtgattatg
    13801 gttgccccca aaggtccggg tcataccgta cgcagtgaat acaaacgcgg cggcggcgtg
    13861 ccttctctga ttgccgttta ccaagacaat tccggcaagg ccaaagacat cgccctgtct
    13921 tatgcggctg ccaacggcgg caccaaaggc ggtgtgattg aaaccacttt ccgcgaagaa
    13981 accgaaaccg atctgttcgg cgaacaagcc gtattgtgcg gtggcgtggc cgagttgatc
    14041 aaagcaggtt ttgaaaccct gaccgaagcc ggttacgcgc ctgaaatggc ttacttcgaa
    14101 tgcctgcacg aaatgaaact gatcgttgac ctgattttcg aaggcggtat tgccaatatg
    14161 aactactcca tttccaacaa tgcggagtac ggcgaatacg ttaccggccc tgaagtggtc
    14221 aatgcttcca gcaaagaagc catgcgcaat gccctgaaac gcatccaaac cggcgaatac
    14281 gcaaaaatgt ttatccaaga gggtaatgtc aactacgcgt ctatgactgc ccgccgccgt
    14341 ttgaatgccg accaccaagt tgaaaaagtc ggcgcacgac tgcgtgccat gatgccttgg
    14401 attacggcca acaaattggt tgaccaagac aaaaactgat tgttttcaaa cgggactgcc
    14461 tacacatcgt gtaggcagtt tgttatatgg ataccgtctg aacatcgtgt tcggacggta
    14521 tctgtgttgc ggatgaattt aaacaggcac agttctgtca gtcgcccaga gccgcgacca
    14581 ttaccgcttt aatcgtgtgc atacggtttt ccgcctgatc gaacacgatg ccggccggac
    14641 tttcgaatac ttcttctgta acttccacac cgttcagccc gaaggtttcg taaatccatt
    14701 cgccgacttt ggtttcgcgg ttgtggaagg cgggcaggca gtgcatgaat ttgacttgcg
    14761 gattgcccga tgccgccatc agttcgggcg taacgcggta atctttcagc aaatcgatgc
    14821 gttcctgcca gacttctttc ggctcgccca tgctgaccca tacgtcagta tgaatgaaac
    14881 cgacaccttt gacggcttca tgcgcgtttt cggtcagggt aatttttgca ccggtttctt
    14941 tggcggcggc gtgtgcggcg gcgataatgc cttcagacgg ccacaggctt tgaggtgcgc
    15001 cgatacgcac gtccatcccc aattttgccc ctaaaatcag cagggaattg cccatgttgt
    15061 aacgcgcgtc gccgacgtag gcaaacgcgg tttggttcaa aggtttgccg ctgtgttcgc
    15121 gcatagtcag tgcgtcggca agcatttgtg tgggatggaa ctcgttggtc agcccgttga
    15181 acacgggtac gcccgcatat tttgccaatt cttcgacagt ttcctgagcg aagccgcgat
    15241 attcgatggc atcgtacatt ctgcctaaga cgcgcgccgt gtctttgatg ctttctttgt
    15301 gcccgatttg gctggcggac ggttccagat aggttgcatc cgcgccttgg tcgcgtgcgg
    15361 cgacttcaaa cgcacagcgt gtgcgcgtgg atgttttttc aaaaatcagg gcgatgtttt
    15421 tccctttcat ccgctgaatc tcgcgccctg cctttttggc gtctttcaac tcggcggcaa
    15481 ggtcgaggta ggtggtgatt tcttccggcg tgaagtccaa aagtttcaga aaatggcggt
    15541 ttttcaggtt cactgtcgtt tcctttatgt gggggcgtga acgccgtatt tgcagcgttt
    15601 gcggaaagaa gggttagcgg aatgttgcaa tgccgtctga aggttgttca gacggcattg
    15661 cggtcaatcc cttactccat acggatgacg gggatcaaac attcacggtc tcggctactt
    15721 cgttgtagct gtcgatttcg ttgaagttca tatagcggta gattttatcg ccctgttcgt
    15781 tgatgatgcc gatattggct tgatattctt caacggtcgg gattttaccc agtttggagc
    15841 aaatcgccgc caactctgcc gagccgaggt aaacgaaggt gtttttaccc aagcggttcg
    15901 ggaagttgcg ggtcgaagtg gacatgacag ttgcaccttc gcgtacttgg gcttggttac
    15961 ccatacacaa cgaacagccc ggcatttcca tacgcgcgcc ggcgcggccg agtacgccgt
    16021 agtgaccttc gtcggacaac tctttcgcgt ccattttggt cggcggcgct acccacaggc
    16081 ggacggggat gtcgctcttg ccttccaaaa gtttggaggc ggcgcggaag tggccgatgt
    16141 tggtcataca agaaccgatg aacacttcgt cgattttggt gccggagcgt tcggacataa
    16201 agcatacgtc gtccgggtcg ttcgggcagg cgataatcgg ctctttgatg tcgtccatgt
    16261 tgatttcaat cacggcggcg tattcggcat ctttatccgc ttcgagcaac tcgggatttg
    16321 ccagccattt ttccatagct ttgatgcggc gttccaaagc gcgcggatct tgatagccgt
    16381 tggcaatcat atttttcatc aacacgacgt tggatttcat gtactcgata atcggctctt
    16441 tattgagctt cacggtacag ccggcggcgg agcgttcggc ggatgcgtcg gtcaattcaa
    16501 aggcttgttc cactttcaaa tcgggcaggc cttcgatttc tagaatgcgg ccggagaaga
    16561 tgtttttctt accggctttg gcaacggtca gcaaaccttg tttaatcgcg taaagcggga
    16621 tggcgttcac caaatcgcgc agggttacgc ccggttgcag cttgccgctg aaacgtacca
    16681 atacggactc gggcatatcg agcggtatta cgcccgttgc ggcggcaaaa gcgaccaagc
    16741 ctgaacctgc ggggaaggaa ataccgatgg ggaaacgggt gtggctgtcg ccgcctgtac
    16801 cgactgtatc aggcaacagc aggcggttga gccacgagtg gatgacgccg tcgcccggac
    16861 gcagggacac gccgccacgg gtagaaataa aggcgggcag ttctttatgg gtttttacat
    16921 cgacaggttt cggataggca gcggtgtggc agaaagactg catcaccata tcggcggaga
    16981 agcccaaaca agccaagtct ttcaactcgt cgcgggtcat cggcccggtc gtatcttgcg
    17041 agccgaccgt cgtcatgcgc ggttcgcagt aagtacccgg acgcacgcct tgtccttcgg
    17101 gcagaccgca ggcgcgaccg accatttttt gcgccaaggt gaaaccgact ttgctttcgg
    17161 caggcgcttg cggcaggcgg aatgcagtag aggcaggcag tttcagggct tcgcgcgctt
    17221 tggcggtcag accgcgaccg ataatcaggt tgatacggcc gccggcttgc acttcgtcca
    17281 gcaatacttg tgatttcaat tcaaactcgg caacagtctc gccgtttttc acgattttac
    17341 cttcataagg aaggatattg acgacatcgc ccattttcag cgcggaaaca tcgacttcaa
    17401 tcggcagcgc gccggagtct tcttgagtgt tgaagaaaat cggcgcgatt ttaccgccca
    17461 agcacacgcc gccgaagcgt ttgttcggca cgaacgggat gtcttcgccg gtatgccaaa
    17521 tgacggagtt ggtcgcggat ttgcgtgaag aaccggtacc gaccacgtca ccgacgtagg
    17581 caaccggatg gcctttggct ttgagttctt ccaacaattt aatcggaccg acttcgcccg
    17641 gtttgtcggg cgtgatgccg tcgcgcgggt ttttcagcat ggccagcgcg tgcagcggaa
    17701 tatcgggacg actccacgcg tcgggcgcgg gagagaggtc gtctgtattg gtttcgccgt
    17761 caactttgaa gacggtaacg gtgatttttt cgggaacttt ggcacgggag gcgaaccatt
    17821 cggcatctgc ccaagattgc aaaacttcct gcgcgtattt gttgcctttt tcggcttttt
    17881 cctgaacgtc gtggaaggaa tcgaacatca gaagcgtatg tttcaagcct ttggcggcaa
    17941 tgggcgcgag tttgtcgtcg tccaagagtt cgattaaggc gtggatattg taaccgccga
    18001 gcatcgtacc taagagttcg gtcgcatatt tgggggaaac cagcgggctg gatgcgctgc
    18061 cttcggcaac ggcagccagg aatgaggctt tgactttggc ggcatcgtcc acaccgggcg
    18121 gaacacggtg ggcaagcagc tcgaccaaga actcgccttc gcctgcgggc gggtttttca
    18181 gcagctcaac caaatcggcg gtttgctgcg cattcaaagg gagggcggga atgccgaggg
    18241 cggcgcgctc ggcggcggct ttacgatagg cttctaacat ctctttgttc ctttttccgt
    18301 ttttcttttg tcgggttgca aatgatctgc gttaaatatt gtaaacaaca tttgtaccgc
    18361 tatcatagac tagttttaac aaaaatggaa ctgttatgac aggtaat
//
LOCUS       DAXDSD010000040        18197 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXDSD010000040 DAXDSD010000000
VERSION     DAXDSD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18197
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.040"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            275..973
                     /locus_tag="ACFB2Q_001047"
     CDS             275..973
                     /locus_tag="ACFB2Q_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC0918086.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1030..2034)
                     /gene="amgK"
                     /locus_tag="ACFB2Q_001048"
     CDS             complement(1030..2034)
                     /gene="amgK"
                     /locus_tag="ACFB2Q_001048"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC0918087.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2104..4509
                     /locus_tag="ACFB2Q_001049"
     CDS             2104..4509
                     /locus_tag="ACFB2Q_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC0918088.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQAFDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMQGQSQV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSQDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            4611..5612
                     /locus_tag="ACFB2Q_001050"
     CDS             4611..5612
                     /locus_tag="ACFB2Q_001050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC0918089.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSKNAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(6090..7970)
                     /locus_tag="ACFB2Q_001051"
     CDS             complement(6090..7970)
                     /locus_tag="ACFB2Q_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFC0918090.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQEGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGIFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQKSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            8126..8758
                     /locus_tag="ACFB2Q_001052"
     CDS             8126..8758
                     /locus_tag="ACFB2Q_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC0918091.1"
                     /translation="MENVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQDHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            8830..10200
                     /gene="purB"
                     /locus_tag="ACFB2Q_001053"
     CDS             8830..10200
                     /gene="purB"
                     /locus_tag="ACFB2Q_001053"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC0918092.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAAPEIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTHGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            10280..12148
                     /locus_tag="ACFB2Q_001054"
     CDS             10280..12148
                     /locus_tag="ACFB2Q_001054"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC0918093.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTIELSTVFLSNIYLGFRLGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRALRHISIILFLILTATSFLPAAFYTDILNQPNTYYLSTLRFPELLVGSLLAVY
                     GQTQNGRRQTENGKRQLLSLLCFGALLVCLFVIDKHDPFIPGITLLLPCLLTVLLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFISFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLMLVGYNLYSRGILKQEHLRPLPG
                     TPVAAENNFPETVLTLGDSHAGHLRGFLDYVGGREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAINQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPENTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHESLLKHSRGGALQ"
     gene            complement(12284..12898)
                     /locus_tag="ACFB2Q_001055"
     CDS             complement(12284..12898)
                     /locus_tag="ACFB2Q_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC0918094.1"
                     /translation="MNFTRLLNQVLSTVQKKGSTFSGSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     ETYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGI"
     gene            complement(12934..15084)
                     /gene="dinG"
                     /locus_tag="ACFB2Q_001056"
     CDS             complement(12934..15084)
                     /gene="dinG"
                     /locus_tag="ACFB2Q_001056"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC0918095.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(15267..16565)
                     /locus_tag="ACFB2Q_001057"
     CDS             complement(15267..16565)
                     /locus_tag="ACFB2Q_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC0918096.1"
                     /translation="MPSETALPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(16555..17466)
                     /locus_tag="ACFB2Q_001058"
     CDS             complement(16555..17466)
                     /locus_tag="ACFB2Q_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC0918097.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            17574..17972
                     /locus_tag="ACFB2Q_001059"
     CDS             17574..17972
                     /locus_tag="ACFB2Q_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC0918098.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            18021..>18197
                     /locus_tag="ACFB2Q_001060"
     CDS             18021..>18197
                     /locus_tag="ACFB2Q_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC0918099.1"
                     /translation="MSRQSLQQAAESRRSVYSLNKNLPVGKDEIVQIVEHAVLHTPFS
                     FNSQSARVVVLFGEE"
BASE COUNT         3892 a         4972 c         5243 g         4090 t
ORIGIN      
        1 aaggggtgta acccttccaa atcagggcaa cgcatagggc ggtgctttat gtgccgtcct
       61 gtgtgttgaa acatcattgt tccgcaacag ccgtatccgt ccggggcggc gcgtattgta
      121 cgacagaaac cgcttcggat ggcatcgggt ttgatgccgt ctgaagcggt ttcccgaaac
      181 gaaaacgtcc tttttcggcg gcaaactgta tcaacacgcg aaaatacaat aaaataccaa
      241 tatccgattt atttccaaca tcacggagag ccgtatgaaa tccagacacc tcgccctcgg
      301 cgttgccgcc ctgttcgccc ttgccgcgtg cgacagcaaa gtccaaacca gcgtccccgc
      361 cgacagcgcg cctgccgctt cggcagccgc cgccccggca ggactggtcg aagggcaaaa
      421 ctacaccgtc cttgccaacc cgattcccca acagcaggca ggcaaggttg aagtgcttga
      481 gtttttcggc tatttttgtc cgcactgcgc ccgcctcgaa cctgttttga gcaaacacgc
      541 caagtctttt aaagacgata tgtacctgcg taccgaacac gtcgtctggc agaaagaaat
      601 gctgccgctg gcacgcctcg ccgccgccgt cgatatggct gccgccgaaa gcaaagatgt
      661 ggcgaacagc catattttcg atgcgatggt caaccaaaaa atcaagctgc aagagccgga
      721 agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac ggcaaaaaag tccttgccgc
      781 ctacgaatcc cccgaaagtc aggcgcgcgc cggcaaaatg caggagctga ccgaaacctt
      841 ccaaatcgac ggtacgccca cggttatcgt cggcggcaaa tataaagtcg aatttgccga
      901 ctgggagtcc ggtatgaaca ccatcgacct tttggcggac aaagtacgtg aagaacaaaa
      961 agccgcgcag taggcccgtt tgaaaaatgc cgtctgaaac ttggttttca gacggcattt
     1021 tgattgggtt tagaacgtaa agcccgtttc cagttcttca tcgccgacca gttcgaccaa
     1081 gagcgcgtag agcggggcga gttcggcata acggcgcgat acgcggcgca gatagtttaa
     1141 gaaacgcggg atttccggac ggtatttgtc tttgccgtcg cggtagtaca ggcgtgcgaa
     1201 gatgcccgcg accttcaagt gccgctgcac gcccatccat tcgaaccggc ggtaaaactc
     1261 gtcaaactcg gcagggacag gcaagccggc agcccgcgcc ttttcccagt agcggataac
     1321 caaatccaaa acaaactctt cttcccactc gataaaggca tcgcgcaaca gcgacaccaa
     1381 atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct aaaacgcccg gcctgccgcg
     1441 cgtcagcatc aggttgcgga cgataaagtc gcggtgcacg tagactttgg gctgggccaa
     1501 caagggcggc agcagcgtat cggcggtttg ctgccaaagt tggcgttgtt tgaatgttaa
     1561 ttcgcgcccc aattcttttg cgacaaacca ttccgggaac aggttgattt cgcgcagcat
     1621 cgtttcacgg tcatattcgg gcaaaacccc ttcacggctt gccttctgca atccgaccag
     1681 ctcgccgatg gcctccagca acagggcttt gtgtgccgct tcgccctgct cctgaagcat
     1741 cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc acaaacccca aatccgtgtc
     1801 cgcgtgcaat acctgcggca cattgaccat gtcaaacagc ttctgcacct tcaaataagg
     1861 cgcgacactc atcttgtcgg gcggcgcatc catacagacg acgctgccgc cgtctgaaaa
     1921 cgccgcacgg aaatagcggc ggaaatcagc atccgccgcc gcgaaggaca gatcgaagtc
     1981 ccgttcggga taaacggtct gaagccaatt tttcagttcg gtttgccgtt gcataacagt
     2041 atccgggcat ttcgggttac aataaacact attctaaccg gtaaaccgac ttgaggggcg
     2101 attttggctc gtttattttc actcaaacca ctggtgctgg cattgggctt ctgtttcggc
     2161 acgcattgtg ccgccgatac cgttgcggcg gaagaggcgg acgggcgtgt cgcagaaggc
     2221 ggtgcgcagg gcgcgtccga atccgcacaa gctttcgatt tgaccctcgg ttcgacctgc
     2281 ctgttttgca gtaacgaaag cggcagcccc gagagaaccg aagccgccgt ccaaggcagc
     2341 ggcgaagcat ccgtccccga agactatacg cgcattgttg ccgacaggat gcaaggacag
     2401 tcgcaggtta aggtgcgcgc ggaaggaagc gttatcatcg aacgggacgg cgcagtcctc
     2461 aataccgatt gggcggatta cgaccagtcg ggcgacaccg ttaccgtagg cgaccggttc
     2521 gccctccaac aggacggtac gctgattcgg ggcgaaaccc tgacctacaa tctcgatcag
     2581 cagaccggcg aagcgcacaa cgtccgtatg gaaaccgaac aaggcggacg gcggctgcaa
     2641 agcgtcagcc gcaccgccga aatgttgggc gaagggcgtt acaaactgac ggaaacccaa
     2701 ttcaacacct gttccgccgg agatgccggc tggtatgtca aggccgcctc tgtcgaagcc
     2761 gatcggggaa aaggcatagg cgttgccaaa cacgccgcct tcgtgttcgg cggcgttccc
     2821 cttttctata cgccttgggc ggacttcccg cttgacggca accgcaaaag cggactgctc
     2881 gtcccgtccg tatctgccgg ttcggacggc gtttcccttt ccgtccccta ttatttcaac
     2941 cttgccccca acttcgatgc cactttcgcc cccggcatta tcggcgaacg cggcgcgacg
     3001 tttgacggac aaatccgtta cctgcgtccc gattacagcg gacagaccga cctgacctgg
     3061 ctgccgcacg ataagaaaag cggcaggaac aaccgctatc aggcaaaatg gcagcaccgg
     3121 cacgacattt ccgacacgct tcaggcgggt gtcgatttca accaagtctc cgacagcggc
     3181 tactaccgcg acttttacgg cggcgaagaa atcgccggca acgtcaacct caaccgccgc
     3241 gtatggctgg attatggcgg cagggcggcg ggaggcagcc tgaatgccgg cctttcggtt
     3301 cagaaatacc agacgctggc aaaccaaagc ggctacaaag acgaacctta cgccatcatg
     3361 ccccgccttt ctgccgattg gcataaaaac gcaggcaggg cgcaaatcgg cgtgtccgca
     3421 caatttacac gcttcagcca agacggccgc caagacggca gccgactggt cgtgtatccc
     3481 ggtatcaaat gggatttcag caacagctgg ggctacgtcc gccccaaact ggggctgcac
     3541 gccacttatt acagcctcga cagtttcggc ggcaaagcat cccgcagcgt cgggcgcgtt
     3601 ttgcccgttg tcaatatcga cggcggcaca accttcgaac gcaatacgcg cctgttcggc
     3661 ggcggagtcg tgcaaaccat cgagccgcgc ctgttctaca actatattcc tgccaaatct
     3721 caaaacgacc tgcccaattt cgattcgtcg gaaagcagct tcggctacgg gcagcttttc
     3781 cgcgaaaacc tttattacgg caacgaccgc atcaacgccg ccaacagcct ttccaccgcc
     3841 gtgcagagcc gtattttgga cggcgcgacg ggggaggagc gtttccgcgc cggtatcggt
     3901 cagaaattct atttcaagga tgatgcggtg atgcttgacg gcagcgtcgg caaaaatccg
     3961 cgcagccgtt ccgactgggt ggcattcgcc tccggcggca taggcgggcg tttcaccctc
     4021 gacagcagca tccactacaa ccaaaacgac aaacgcgccg aacattacgc cgtcggcgca
     4081 ggctaccgcc ccgcccccgg aaaagtgttg aacgcccgct acaaatacgg gcgcaacgaa
     4141 aaaatctacc tgcaggcgga cggttcctat ttttacgaca aactcagcca gctcgacctg
     4201 tccgcacaat ggccgctgac gcgcaacctg tctgccgtcg tccgctacaa ctacggtttt
     4261 gaagccaaaa aaccgataga aatgcttgcc ggtgcagaat acaaaagcag ttgcggctgc
     4321 tggggcgcgg gcgtgtacgc ccaacgctac gttaccggcg aaaacaccta caaaaacgcc
     4381 gtcttttttt cacttcagtt gaaagacctc agcagcgtcg gcagaaaccc cgcaggcagg
     4441 atggatgtcg ccgttcccgg ctacatcccc gcccactctc tttccgccgg acgcaacaaa
     4501 cggccctgac cgtcggaaac ccggcaggag caccgttcct gcacaagata gcattccacc
     4561 gacaacccca aacccgccat caaaggcagg attcaaacga taaggaaaga atgatgaaaa
     4621 tcaaagccct gatgattgcc gccgcattgc tggcagcagc cgatgtccac gccgcaccgc
     4681 aaaaggcaaa aaccgcgccc gccaaagcgg tcaaagctgc cgccacggcg caaaaagaag
     4741 ccgcacccgc acaacagcag ggcggtatcc gcttttcaga cggcattgcc gccgttgccg
     4801 acaacgaagt catcaccaac cgccggcttg ccgaagccgt tgccgaagcc aaagccaccc
     4861 tgcccgaaga cgcgcagata agtgaatccg agctgtcccg acaggtgctg atgcagcttg
     4921 tcaaccaatc cctgattgta caggcgggca aacgccgcaa cattcaggca agtgaagcgg
     4981 aaatcgatgc cgtcgtcgca caaaatcccg ccctcaaaaa cctcagcccc acccaacgcc
     5041 gcgagcttgc cgacaacatc attgccgaaa aagtccgcca gcaggcagtg atgcaaaaca
     5101 gccgcgtgag cgaagctgaa attgatgcct tcctcgagca ggcgcaaaaa caaggcatca
     5161 ccctgcccga aggcgcaccg ttgcgccaat accgcgccca acacatcctg attaaagccg
     5221 acagcaaaaa cgccgccgtc ggcgcggaaa gtaccatccg caaaatctac gaccaggcgc
     5281 gcaacggcac ggatttcgca ggtttggcac gccgatattc ccaagacgca agcgcgggca
     5341 atggcggcga cttgggctgg tttgccgacg gcgtaatggt tcccgccttt gaagaagccg
     5401 tccacgcgct caaacccgga caggtcggcg cgcctgtccg cacccaattc ggctggcaca
     5461 tcatcaaatt gaacgaagtg cgcgatgccg gcacgccgga ggaacgtatc cgcaattccg
     5521 tgcggcaata catcttccaa caaaaagccg gacaggcaac cgtcaacctg ttgcgcgacc
     5581 tgcattccgg cgcgtatgtc gacatccgct aaggcggttt gaatccaaaa gtcataccga
     5641 tcgggcaaaa gtccgggcgg tatggctttt ttgaatttcg agttactttt acaccgccat
     5701 tcgtcattcc cgcgcaggcg ggaatctagc aacgaaaagt aacaggaatt tatcgggaat
     5761 ggccggagtt taaaggtctg gattcccgcc ttcgcgggaa tgacgattca gaagttacac
     5821 gaaactcaaa aaaaaacgaa accgaacgaa ccggattccc gcttttacgg gaatgacggc
     5881 atatcggttt ccgtgaggac aaacctagat tcccgcctgc gcgggaatga cgggatttta
     5941 gattgcgggt atttatcggg aatggcgatt taaaaattac ctaaaactaa acttaaaagc
     6001 aactgaaact taaaaaacta aaatttcgcc tgcgtgggaa ggatgggatt tcagatttct
     6061 gtttttgtgg gaatttgccg tatcgggctt cagacggcat tgcctgccgt tttgcccccg
     6121 ggtgcggctg ccttgatgta gttgagcgag acaaactgct tctcggcatc caattcggtg
     6181 attttaaaca atgcctgcga tttgggcagc gcgtcaaacg gaataccggt cgcgcgcgtt
     6241 gtcagtggca gaccttcgat gcggacgagg tcttctttca gaatcgtcgc ggtcagttcg
     6301 ctgatgcctt gttgttgcag gtacacgagg ctccagtagg cttccatctg ccgttggaag
     6361 tcggcgtagg cggcgtaggc ggtgtcgaaa tcgcgcaggg cggcgaagag cgcggcatcg
     6421 cttttttgat acagcggctc ggcagtgtcg tcgatcaggc tgatcagctg cttttggttg
     6481 atgtagtcgg cggcgcggcg cagcggcgag gtaaaccagc cgtaatgctg cacgcccatg
     6541 ccgatgtgcg gctcggattg ggtgctcatg cgcactttgc ctgccggttg gacgcggaaa
     6601 atgccgggca gcccgttttc gtctagcatt tgcgcccaag tgctgttggc aagaatcatc
     6661 atctcgctga ccagcgtatc gatgggcgaa ccgcgttcgc ggcggacgac ggagacgttg
     6721 ccttcttcat ccaattcgat gctgtaatcg tattgcggcg cgcggtcggg ttcgtatttg
     6781 ccgcgcgctt tttgcagggc ggtggcgaat tgatagaacc aaatcaggtc ttgatggtgg
     6841 gcgaacatca tttcgcctgc ttggtccaag ccggtttctg cgttgaaatg cggctcgatg
     6901 gcttggatac gcaggtttgc ggcgatgttg acggcttcga ttttgcaggt cggctcgccg
     6961 acgttgaact cgccgtccac atcgaaataa atgctgacgg cagggcggta tgcgcctgca
     7021 tcgaggctga acgcggtaat ccagttttcg ggcagcatcg tgattttgcc gccggggaaa
     7081 taaaccgtac tcaaccgctg cataatgatt tgttccattc cgccgccttg tcggacggca
     7141 agcgacggcg cggcgatgtg gatgccgacg cgcttcgtgc cgttgcccaa atcggtcagg
     7201 ctcaaggcat cgtccacttc ggtggtcgat tcgtcgtcaa tggaaaaggc ggtaacgtcg
     7261 gccttgggca ggtcgggcat ttcgggaagg gagaggtcgg ggaaacctgt tcctttgggg
     7321 aagtatttga tttcaaagcc ttcttgcagg tattgcggaa tggacgtaat gccgcccgtt
     7381 tttttcgcca gttcgtaggc ggaggttttc agtgcgtcgg cggctttggt aaaggctttg
     7441 taggtcagcg actgtttgtc gggcgcgtgt aagatggttc tcaaatccgc cgcgatttca
     7501 gacggcatct cgccgcgttt caaggcttcc gcccaagcgt cgatttgcgc gtcttgctgt
     7561 tttttgcgtt cgatggcggc aagcgcttgt ttcaaagttt cttcgggcgc ggctttgaac
     7621 acgcctttgg cttttttgta gaaatacacc ggcgcggcgt acagcgcaat caaagtcgcc
     7681 gccagctcgg ttttggtcgg cgcatggccg taatattctt cggcgatggc ttcggcggta
     7741 aactcctctt cgccgcatac ttcccacaat aaatcggtgt cgatgtccgc cgcctgcgcc
     7801 tgagcgtttt ccaaaaacgc cgccatatcg ccgtcaaact cggcaaagac attgtttgcc
     7861 ttcactttgg tgcgtttgcc gtgttgggta tcgacttggt aggtagcgtc gtttttttgg
     7921 acgacgacgg caactttgaa ctggccggac tcttcgtaaa aaatattcat ttttcggatt
     7981 tttctgtgga aactcaagcg ggcgatttta gcagattacc gaaaatgccg tctgaaaaaa
     8041 ggttggggga aggcgggtgc ggctttgcgg tgcttgcgtt gcgtttttga cggggtacaa
     8101 tcgccgtttt tgaacggagg acggaatgga gaatgtaaac cgcgtgccgg agcaggcacg
     8161 ttatgatgcc gaacgcaggc aggcagacga agcattggcg gacgtgtttc cggcagtcag
     8221 tattttcggc agcgcgcgca cgccgcagga tcatgcggat tatgcgtttg cctgccgtct
     8281 ggcgcggcgg ctgtcggatt cgggtattgc cgtcatttcg ggcggcgggc cggggattat
     8341 ggaggcggca aacaagggcg cgtttgccgg gaagtcggtt tcggtagggc tgaacattgc
     8401 cctgccgcac gagcagaaac cgaatccgta tcaggacatc gccttgcggt tttcccgttt
     8461 tgccgaacgc aaggcagtgt ttttccgcta ttcccaagca tatgtcgtga tgccgggcgg
     8521 cttcgggacg ctggacgaat tgtttgaaat cctgaccttg gtgcagacgg gcaaagtgcc
     8581 gccgcgtccg gtcgttttgg ttggaaaggc gttttggtcg ggcttggcgg agtggataaa
     8641 cgcgcagctt ttggcgcgcg gtatgatttc cgaaggggcc gcttctttgt tctccatatc
     8701 ggacgacgaa gacgaaatca ttgcgtatct gtcggaacac gggcttcaga cggcatagcg
     8761 tcctgagagt gatgtataat tgcaaacaat ttgtcaattt ttgatgtctt ttccgaacag
     8821 gatgccgata tgatcaaccc catcgcctcg ctttcccccc tagacggccg ttatgcccaa
     8881 tccgttgaag cattgcgccc gattttttca gagtacggac tgatgaaggc gcgcgtcaaa
     8941 gtcgaattaa actggctcaa agccctggcc gccgcgccgg agattgccga agtgccgccc
     9001 ttcagtgccg aaacgcttgc cgaaatcgac aaagtcatcg aaaacttttc attagaagac
     9061 gcggccgccg tcaaagccat cgaagccacg accaatcatg atgtcaaagc catcgaatat
     9121 tggctgaaaa aacgttttgc cgaagtgccg gaagtcgccg ccgtgagcga gttcatccac
     9181 ttcgcctgca ccagcgaaga catcaacaac ctgtcccacg ccctgatgct gcaagaggcg
     9241 cgcgaaaccg tcatcctgcc caaacttgcc gaaatcatcg gcaaactcac cgctatggcg
     9301 cacgaccttg ccgccgtccc gatgatgagc cgcacccacg gccagcccgc cacaccgacc
     9361 actttgggca aagaaaccgc caatgtcgtg taccgcctgc aacgccagtt caaaatcctt
     9421 caggcgcaag aattcctcgg caaaatcaac ggcgcggtcg gcaactacaa cgcccatatg
     9481 gccgcctatc ccgatgtgga ttgggaaaca cactgccgca acttcgtcga aatcagcctc
     9541 ggtctgacct tcaaccccta caccatccaa atcgagccgc acgactatat ggcggagttt
     9601 ttccaaaccc tcagccgtgt caacaccatt ctgatcgact ttaaccgcga cgtttggggt
     9661 tatatttcat tgggttattt caaacaaaaa gtcaaagcgg gcgaagtcgg ctcttccacc
     9721 atgccgcaca aagtcaaccc catcgacttt gaaaactccg agggcaacct cggtatggca
     9781 aacgccgtat tgggcttttt gtccgaaaaa ctgccgattt cccgctggca gcgcgatttg
     9841 accgacagca ccgtattgcg caatatgggc gtgggcgtgg gctatgccgt gttgggtttc
     9901 gccgcccacc tgcgcggtct gaacaagctc gaacccaacc ctgccgcgct tgccgccgat
     9961 ttggatgtca cttgggagct gctcgccgag ccgattcaaa ccgtaatgcg ccgttacggt
    10021 gtcgccaatc cttacgagaa gctgaaagac ctgacgcacg gtaaagacgg cattacgccc
    10081 gaagtgttga aactctttat cgaatcgttg gaaattccgg cagaagccaa agccaaattg
    10141 cttgagctga cccctgcgtt gtatgtgggc aaggctgaag cgttggcgaa acggatttga
    10201 gtgcttattg aaaccgatgc cgtccgaacg cgcgttcaga cggcattttt aagataacgg
    10261 gacatacggg gcgatattta tgcaagctgt ccgatacagg cctgaaattg acggattgcg
    10321 ggccgtcgcc gtgctatccg tcattatttt ccacctgaat aaccgctggc tgcccggagg
    10381 attcctgggg gtggacattt tctttgtcat ctcgggattc ctcattaccg gcatcattct
    10441 ttctgaaata cagaacggtt ctttttcttt ccgggatttt tatacccgca ggattaagcg
    10501 gatttatcct gcttttattg cggccgtgtc cctggcttcg gtgattgcct ctcaaatctt
    10561 cctttacgaa gatttcaacc aaatgaggaa aaccatagag ctttctacgg tttttttgtc
    10621 caatatttat ttggggttcc gattggggta tttcgatttg agtgccgacg agaaccccgt
    10681 actgcatatc tggtctttgg cagtagagga acagtattac ctcctgtatc ctcttttgct
    10741 gatattctgc tgcaaaaaga caaaatcgct acgggcgttg cgccacatca gcatcatcct
    10801 gttcctgatt ttgactgcca catcgttttt gccggccgcg ttttataccg acatcctcaa
    10861 ccaacccaat acttattacc tttcgacact gaggtttccc gagctgttgg tgggttcgct
    10921 gttggcggtt tacgggcaaa cgcaaaacgg cagacggcaa acagaaaatg gaaaacggca
    10981 gttgctttca ttactctgtt tcggcgcatt gcttgtctgc ctgttcgtga tcgacaaaca
    11041 cgatccgttt atcccgggaa taaccctgct ccttccctgc ctgctgacgg tgctgcttat
    11101 ccggagtatg caatacggga cacttccgac ccgcatcctg tcggcaagcc ccatcgtatt
    11161 tgtcggcaaa atctcttatt ccctatacct gtaccattgg atttttattt ccttcgccca
    11221 ttacattaca ggcgacaaac agctcggatt gcctgccgta tcggcggttg ccgcgttgac
    11281 ggccggattt tccctgttga gctattattt gattgaacag ccgcttagaa aacggaagat
    11341 gaccttcaaa aaggcatttt tctgccttta tctcgccccg tccctgatgc ttgtcggtta
    11401 caacctgtat tcaagaggga tattgaaaca ggaacacctc cgcccgctgc ccggcacgcc
    11461 cgttgctgcg gaaaataatt ttccggaaac cgtcttgacc ctcggcgact cgcacgccgg
    11521 acacctgcgg ggttttctgg attatgtcgg cggcagggaa gggtggaaag ctaaaatcct
    11581 gtccctcgat tcggagtgtt tggtttgggt ggatgagaag ctggcagaca acccgttgtg
    11641 ccgaaaatac cgggatgaag ttgaaaaagc cgaagccgtt ttcattgccc aattctatga
    11701 tttgaggatg ggcggccagc ccgtgccgag atttgaagcg caatccttcc tgatacccgg
    11761 attccaagcc cgattcaggg aaaccgtcaa aaggatagcc gccgtcaaac ccgtctatgt
    11821 ttttgcaaac aacacatcaa tcagccgttc gcccctgagg gaggaaaaat tgaaaagatt
    11881 tgctataaac caatatctgc gccccattca ggctatgggc gacatcggca agagcaatca
    11941 ggcggtcttt gatttggtta aagatattcc caatgtgcat tgggtggacg cacaaaaata
    12001 cctgcctgaa aacacggtcg aaatacacgg acgctatctt tacggcgacc aagaccacct
    12061 gacctatttc ggttcttatt atatggggcg ggaattccac aaacacgaaa gcctgctcaa
    12121 gcattcccga ggcggcgcat tgcagtagcc tgccttcttg ccggatattg cctttggcag
    12181 cctatgccgc tgtttgccct ttgaggcggc ttttattttg ccgttttgag gccggggtcg
    12241 ggaataaccg ttttttgatg attttccctc cccggctgtg tcatcaaatc cctagttgcc
    12301 tttccaaact ctccaccaga ttgtcatcca gtttcaaagc ctgcgacagg cgggcgagga
    12361 agacggtttc tttccgcgac aaatcggcac agaccaacct tgccgccaga taggtctccg
    12421 ccgccaacgc ctcatcgttg ccgacggcgg cggcgatgtc cccgatgctt gcgggcaggc
    12481 ggtattcggc ggcgagccat gcggcagttt cggggtctgt gccgctttcc tgttcgatag
    12541 tccggcgttc ggcttcgtct atcatgccgt ctgaagcggc ggcggctatc atggtacgca
    12601 atacggtgcg gctgtatgtt tcttcggttt ttccggcagg ttggaaatcg ctttgtgtta
    12661 cggttgcccg ccctttgttt tgctgccaca tctgatagcc ccggtaggcg aggtagccca
    12721 aagcggcggt cgaaccgatt ttggtgatgg ttttgcggtt tttaccgttc agcagcatgg
    12781 aggcgacacc ggcaaccagc gcgcctccgc cgaatgaatt gagcgggctg ccggagaatg
    12841 tgctgccttt tttttgaacc gtgcttaaga cttggttgag caaccgggtg aagttcatga
    12901 tttttccttt ctgttgcggg aagggcggta tgtttaccct atccttttaa acggtggcag
    12961 gccggccaat aattgttggc cgtaccgctg cgttttgatg cggttgtcga ggatggttac
    13021 gcggccgtag tcttgttcgg tgcggatgag gcggccgacg gcctggatga gtttgatgcc
    13081 ggcttcgggg acggtgattt cgatgaaggg gttgccgccg cgttgttcta tccaacggtt
    13141 ttgggttttt tcgatggggt tgtcgggcat ggcgaagggg agtttggcga tgatgacttg
    13201 cacgcaggcg gtgccgggca ggtcgagccc ttcggcgaag ctgtcgagtc cgaagatgat
    13261 gctggctttg ccttcttcta tggcttggtg gtgtttttgc aggaggacgg ctttgggtaa
    13321 ttcgccttgt acgagcaaga gcggcaggta gtctccgggc aggtgcaggg cgacatcctg
    13381 catttgtttg cgcgaggaaa acaggacgag tgtgccgatg gcttcggtgg gcgaaatcag
    13441 cttgggcagc cattcgatga cggcggcggt gtgggcttcg gggtctttgg ggctggcgta
    13501 tatggagggg atgtagagtt cgccctgttt ttcaaagtca aaggggcttt tgagggcgag
    13561 ggtggtggtt tcgggcagcc acagcaggcc ggtttggcgc aaaatcaagt tgaagctgcc
    13621 gagcgattgc agggtggccg aagtcagcac cgcgcctgcc gcgcgccgcc acaggctgtt
    13681 ggcaaggtgg gacgcgctgc tgatggggct ggcattgaaa atatagtcgt ttttgtcgtc
    13741 ggcacggcgg gctatccatt tcgccaacgg ctcctcgccc tcaatggaaa cagtggagag
    13801 caaatcccaa accgcgctga tttgttcgat acgggcgata aaaaggccga actcgccggt
    13861 caggcggtcg atgagcgcac cgtcctgttc tttttcgcgg cgtgcggcgg aaagcgcatc
    13921 gttcagcccg acaacgtgtt tgagcaggct gcgtgcagca atggccgtat tggaaacggt
    13981 ggtttcgagg ccttcgggga ttttgccgtc ttcccacagc caagtcggtt cgctgttggt
    14041 tcgtctgtcg ttttcagaca gccccatact taaagacggc tcttccgcca aatggaattg
    14101 ccattcgtgc aggctgtcga gcaaggatgc ggcggcttcg tcggcaaggt tggcaagttc
    14161 ggctttatcg gtaagcgcgg caattttgcc ggtaagctgc ggcagttttt ccagcgtcca
    14221 aacggcaata ttccatgaat gttcggcggc aaaacggctg agggcttttt tgggcaggtg
    14281 gtgcgcttcg tctatgcaat agaaactgtt ttcgggcgca ggcagaatca cgccgccgcc
    14341 catgctgatg tcggcaagca gaagatcgtg gttggcaaca acgacatcga cggtttccaa
    14401 catatcgcgt gccaggtaaa aagggcattc cggacggttg ggacaggcgg ctttcaggca
    14461 gccgtggcgg tcgttggtca ctttgagcca aatcgcgtca tcgatttttt ccggccaagc
    14521 gtcgcggtcg ccgttgaacc gtcgggcgga aaattcgtcg gcgatgtcgc gcagcagctt
    14581 caattcttcc ggcttgggtt tgctgtccca caagacggcg ggggcttcaa agccgagcag
    14641 gttttgctgg gcgttgcttt gcgtcagttg atagagtttg taggggcaga gatagcggcc
    14701 gcgccctttg gcaagtgcga aggtcagttc caaaccgctt ttttcgacca gaaacggcag
    14761 gtcgcggtct accaactgct cctgcaaggc aaccgtcgcg ctgctcacaa tcagccgctt
    14821 gccgcgtgtt tgcgccatga tgccgcccgc aagcaggtag gcaagcgatt tgcccacgcc
    14881 ggtcggccct tcgattacgg caatgctttc gccctcgcgc ttgggcggct cgccgccttc
    14941 ttcgcgcgtc aacgtccgcg aaaaagcgtt ggcaaccgcc gcaatcattt cccgctgcga
    15001 agcacgcgga cggaaaccgg gcaggttttt gccgatattt tggtaatggt cgcggatggc
    15061 gtttttttct aaatcggtga gcatagtatc ggtgggcgga tgatttgggg tcattttaac
    15121 attgcgcgga tgcggtacgg tatcgttgtc ggacggtggt tcgggcatat ctgattattg
    15181 aaagtaaaaa aaaacggaag atattgggtc atccgtacaa aatgccgtct gaagcgggaa
    15241 acgggcttca gacggcattg ccgcgcttat acgccggcga cggggcgggc gggatagccg
    15301 tgggtatcga ccgaacaggc aaaccgccaa ggcgtgtgga cggtgtcggc ggacaggtgg
    15361 gcgagttcgg gaatgtgccg tttgataaaa gtaccgtccg ggtcggtacg gtatgaggcg
    15421 gcggcaatgt cggggcaggt gtgccgtgag gcggcaagcc gccagttgcc ttggttgatt
    15481 gccgcatcga aatcggtcag ctgccgggca aaccatatct cgccttcgcg gcgggggagg
    15541 tttaaaacgt ggcagaaaaa atccgcgctc aagcgtctca gggcggggtg gaggctgccg
    15601 gttttgtgca aacagcgcat cgcggcatcg ataatcggaa tgccggtccg gccctgctgc
    15661 caaagcgtca ggcgcagggt gtgttccgga ttgccgtctg aagggtcgtc atccgcgtgc
    15721 tgcaaggcaa gttgaaggaa aaaatcgcgg cggatgatgt tgtccgccca cgcgttcaga
    15781 cggcgttcga ggctttcccg cgcgagcagg cgcggcgaga tgcagccgac actcaaatac
    15841 gcgcccatca gcgaagtgtt tttgcgcgaa gggaaatcct ttaaaacgga gtaggaagcc
    15901 gcctgttcga gaaaccgccg ccactgccgc caagccgccg tttcgccgcc gttttgcggc
    15961 aggaagatgc cgtctgaaag cgcggcaggc ggcggggcgg gcaggttttc ggggaagggt
    16021 tggcggtatg ccgcgaatag gtccgaaccg gcggggggct gcttggaaaa gcggtcgagc
    16081 catacttcgc ggtagcggtt gaaatcggtg tgtgccgtac cgttgtctgg tgtcaggtcg
    16141 gttttgccga aaacggaacg gtcgttgacg aaggttaacg cgatgccgtg tttgtccaat
    16201 tcgtgccaaa gggcgttgtc ggcgagtttg tcggcaaaag tatgggattc gtcggcgatg
    16261 acggcgcgga tattgaggcg gacggcgagc cggacaagcc cggcaggcga tgccgccgtg
    16321 tagagcggga tgccgcgcct tgccagccct tgggcgagtt cggcggcgga ttggcggtag
    16381 aacgcggcgc ggcgcgggtt gtctgtttcg gcatcgtcaa tccaaatgcc gataacgggc
    16441 gaaccttcgg caacggcggc gcataaggcg gcgttgtcgc ggatgcggag gttttggcgg
    16501 aaccagacga gcgtgtgcgc ggcgcgcgtg tccgcataaa gggggagggc ggtttcagac
    16561 ggcatttcgg cagcctttcc tgctggcgat tttttcgttc agaaaatcga tgaaactgcg
    16621 gactttcgca ctcaaaaatg ccctgtccgc ataaacggcg ttcagccggt cggtcgggac
    16681 ggcgtatccg ggcagcagcc tcaccagcgg gccgcagcgc aaatcgtgtt ccgccgccca
    16741 aagcggctga taaccgatgc acgcgcccgc cttaatcatt tcgcgcatca tcagcgtgtt
    16801 gtcggtacgg atgacggggg tcagttcaag ccggtatttc ttgccgtccg atttgcgggt
    16861 gaggtcgagt ttctgctggt tggtgtaggt cggcaggacg gcaggcagcc ccgccacttc
    16921 ttccggtgtt tccggcacgc cgttgcgcct caggaagtcg ggcgaggcaa gcagggcaaa
    16981 ttcgatttcc gccagcgggc gcgcaatcag cgaaggggaa agggtttggg aaacgcgcaa
    17041 cgccaaatcc acgccttcgg caatcaaatc gacgtggcgg ttgtccaaaa tcagttccaa
    17101 cgccacttcg ggataacgtt cgcggtattc cgccagccag ttgcatatct ggctgccggc
    17161 aaaccacagc ggcatcgtta cgcgcagcag cccctgcggt ttttccgtcc ccccggcggc
    17221 tttttgcgcg gcatcgtcga gcgtgtcgag cgcgtaactg cattgccggt agtattcttc
    17281 cccggcttcg gtcaggctga ggttgcggct gttgtggtgc aggagtttgg cttggacggt
    17341 gttttccaag tgactgacgt gtttgcttgc cattgcggtg gagatgccga gcgcgtcggc
    17401 ggcgcgggtg aagccgccgc tttggacgac ttggcggaaa actttgaggc tgaacagggt
    17461 gtccatattt tcttgtgtgg aaaagttgta tcaataaaag cagtatatat ttgaaaaagg
    17521 gaaacatcta tactctgccg cctgaaatga agacaaatat caaaggagct tttatgtccg
    17581 attgctgcaa ccgtatccaa ccggttttgc tttctgtttt gcgtatcgta accgcctacc
    17641 tgtttttgtt gcacggtacg tcgaaaatct tcgccttccc cattgaaatg ggcagcggtt
    17701 cgcccggcgg gctgttgctg cttgccggta ttttagaaat tgtcggcggc attttgctgg
    17761 tgttgggcct gtttgcgcgc cctgccgcgt ttgttttgtc cggccagatg gcggttgcct
    17821 attttatggc gcacgcttcc ggaaatgctt tgttcccgat tgccaacggc ggcgagtccg
    17881 cagtgctgtt ctgcttcgta tttctctata tcgcggcagc gggcggcgga gcatggtcgc
    17941 tggacaggct gtttttcaag cgtaaagcct gaaccggact gcctaaagtg tattttgttg
    18001 aatgtttttg aggaaaagaa atgagccgtc aatccctgca acaggctgcc gaaagccgcc
    18061 gttccgttta ttcgttaaat aaaaacctgc ccgttggcaa agatgaaatc gtccaaatcg
    18121 tcgaacacgc cgttttgcac acaccttttt cgttcaattc ccaatctgcc cgcgtggtcg
    18181 tgctgtttgg cgaagag
//
LOCUS       DAXDSD010000041        18157 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXDSD010000041 DAXDSD010000000
VERSION     DAXDSD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18157
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.041"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            212..2218
                     /locus_tag="ACFB2Q_001061"
     CDS             212..2218
                     /locus_tag="ACFB2Q_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC0918100.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
     gene            complement(2341..3432)
                     /gene="mltB"
                     /locus_tag="ACFB2Q_001062"
     CDS             complement(2341..3432)
                     /gene="mltB"
                     /locus_tag="ACFB2Q_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC0918101.1"
                     /translation="MEKRKILPLAICLAALSACTAMEARTPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGRAKFHGARRFYAENRAVIDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(3580..5007)
                     /locus_tag="ACFB2Q_001063"
     CDS             complement(3580..5007)
                     /locus_tag="ACFB2Q_001063"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC0918102.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEAQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            5192..8896
                     /gene="mfd"
                     /locus_tag="ACFB2Q_001064"
     CDS             5192..8896
                     /gene="mfd"
                     /locus_tag="ACFB2Q_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC0918103.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDGIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDDPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQKINAIHEELVDRFGLTEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            9122..9424
                     /locus_tag="ACFB2Q_001065"
     CDS             9122..9424
                     /locus_tag="ACFB2Q_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HFC0918104.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            9586..9969
                     /gene="panD"
                     /locus_tag="ACFB2Q_001066"
     CDS             9586..9969
                     /gene="panD"
                     /locus_tag="ACFB2Q_001066"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC0918105.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            9991..10833
                     /gene="kdsA"
                     /locus_tag="ACFB2Q_001067"
     CDS             9991..10833
                     /gene="kdsA"
                     /locus_tag="ACFB2Q_001067"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC0918106.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            10848..11288
                     /locus_tag="ACFB2Q_001068"
     CDS             10848..11288
                     /locus_tag="ACFB2Q_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC0918107.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            11335..12621
                     /gene="eno"
                     /locus_tag="ACFB2Q_001069"
     CDS             11335..12621
                     /gene="eno"
                     /locus_tag="ACFB2Q_001069"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC0918108.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            12789..13067
                     /gene="ftsB"
                     /locus_tag="ACFB2Q_001070"
     CDS             12789..13067
                     /gene="ftsB"
                     /locus_tag="ACFB2Q_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC0918109.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(13108..13398)
                     /gene="yfaE"
                     /locus_tag="ACFB2Q_001071"
     CDS             complement(13108..13398)
                     /gene="yfaE"
                     /locus_tag="ACFB2Q_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC0918110.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(13519..14652)
                     /gene="nrdB"
                     /locus_tag="ACFB2Q_001072"
     CDS             complement(13519..14652)
                     /gene="nrdB"
                     /locus_tag="ACFB2Q_001072"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC0918111.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(14965..17244)
                     /gene="nrdA"
                     /locus_tag="ACFB2Q_001073"
     CDS             complement(14965..17244)
                     /gene="nrdA"
                     /locus_tag="ACFB2Q_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC0918112.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKITDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17775..18086
                     /locus_tag="ACFB2Q_001074"
     CDS             17775..18086
                     /locus_tag="ACFB2Q_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC0918113.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
BASE COUNT         4402 a         5118 c         4488 g         4149 t
ORIGIN      
        1 aacatatctg ctaatctagt acagacccgc cgaaattgtt ataatcgaaa aaattaaaaa
       61 tcaatacgga ttattctatc agattgattt atcgatattt attatatcat taatatagtt
      121 tgatttcaaa ccggcggaaa atgcgccgcc cgccgcagat gcgccctccc cgccatcggg
      181 gcgtttaacg ccggattgcc gatgcggtac aatggcagat atgaagaaaa ttacccctca
      241 aaacctgcgt cccctgcttt cggaaagctt gggacatacc gattttgtca acgtcctcaa
      301 cgcattgatt aaatttttgc gccgtggcgg caaaaaatgt gcgggggagc gtttcgacct
      361 gattatcgac acattcaaac aagacaggga attactgtcc cgcttcagcc ggtgttttta
      421 catctggctc gcgcaaatac acatttatcc ggcactcatc aaactcggca ttttctcgcg
      481 ccacagcttt gcccgggaaa tgggcatacg catatacgaa cgcttcaacc cgtcatataa
      541 agattttgcc aacttgggcg aagtcttcct ttatcttttc cattccgaaa acgacgacaa
      601 atggctgcaa acgctcaata tccgccaatg gctggtttta tacgaactca tccgtagcca
      661 cgccgagccg tccaaattgc agacggcggg catccgcctt gccgatgcgc gtttgcgcgc
      721 catcgaaatg ctgtctgtct ggacggcatc cgaagccatc gaacccgatc tcatccgtat
      781 cgccccgcgc ctgctggaag ccgattcttc cttcgtcgcc ctccaacgcg aaaccgccaa
      841 actggtcgaa cactaccgca acggcaccgc gccttacgac accgcccacc tcgaagtgat
      901 gttcgaccaa tgtttcagcc agattgacta tttgcgtcgc aaagggacgg gcgccggctc
      961 cggttcgtcg gtcaaagtcg cccacctgct cgaacgactc cggcagaccg taggccgtct
     1021 gaagctgctc acagacatac aaaccggtgc cggcaacagc aaccgcctga ccatcgccct
     1081 gatgaactcc ctcatctacg cggcggtcga acaatacagc acccgccacc tgcgccgcag
     1141 cagcatccgt atgctcgccc gcagcattac cgaaaacaaa agccaccacg gcgaacacta
     1201 catcacccgc aaccgcaaag aatatttcaa aatgttctac tcggcagcag gcggcggcgg
     1261 catcatcgcc ctgatggcgc tgctcaaaat ccgcatcggc acactcgacc tcagtccctt
     1321 cctcgcttcc ttgtcggctg ggttcaacta cggcatcggc tttatgatca tccatatgct
     1381 gcactgcacc gtcgccacca agcagcccgc gatgactgcc gccagctttg ccgaacaggt
     1441 cgatctcaac gaaggcggca aagcggtgga caacaaactc tccaagctcc tcatcgacgt
     1501 atgccgctcc caaagcgtcg ccgtcttcgg caacgtttcc atcgccatcc ttttggcgtg
     1561 cgccatatcg ttcggctatg cccatctgta ccggctgccc atactcgatg cccacaccgc
     1621 cgcctaccag ttcaaatcca tagacatcat caatcatcct accctatggt atgccgctat
     1681 tgcaggcttg tggctgttct gctccggcat catcgcaggt tttttcgaca accgcgccga
     1741 ctacctcaac ctgcgccaac gcctgccctt caatcccttg ctgcgtaaaa tcatgcgccc
     1801 cgggccccgc cgcgtcctcg ccgcctacat ccacaaacac tacggctcgc tggtcggcaa
     1861 cttcatcttc ggaatgctct tgggtatgac cggctatttc ggacacctcc tcgggctgcc
     1921 gctggacatc cgccacgtcg ccttttcctc cgccaacctc ggctatgccg ccgtcagcgg
     1981 caacgtcggc tttggtacat tcgtactcgg cattttcagc gtcctcgcca tcggcttggt
     2041 caacctctgc gtcagcttca gcctcgccct cttcgtcgcc ctgcgctcgc gcggcacaaa
     2101 aatcggcagc atccgcaacc tgatcaaaag tttttggaat cagattaaaa gcaatccttg
     2161 catacttttc ctcccgcccg ccaaaggaca gggacatcct ccttcggaca agccttgacc
     2221 ggcaatgccg tctgaaacgg gattcgcccc gaataccgcc ctgatgcggg aaatccccat
     2281 aaaaggatgc aaaaatgccg tctgaaccga aacgtggttc agacagcatt ttaaaaaaca
     2341 ttacaatccc gggccgccga gcgaattggc aatgtccctg accgccgtta catacatccg
     2401 gctgtggttg tactgccata ccgtataaaa attgttcaag cccaaataat attcaaacac
     2461 gccgggtgcg gtttccagtt tgaacaaaac cgccttttca tcatctgcga gcgtttcccc
     2521 ggggatgatg ccgtacgcct tcaaatccgc caccgtccgc gtcagggcgg ttttttcgcc
     2581 aatgattgcc tgaacatccg cacccggcgc caacgtcgcc gacaccaaca ttttaccgcc
     2641 cgtgcgccaa ccgtgctgct tcatataatt ggcaaccgat gccgcgacat caccgacgtt
     2701 gccccatata tcccgatgtc cgtccccgtc ataatccacc gcccatttcc ggtagctcga
     2761 aggcataaat tgcggcatac ccattgcgcc cgcatagctg cccttaaagg cgaaaacatc
     2821 gccgccttct tcttttgcca gctttaaaag ctcgaccaat tctttttgga aaaacccggc
     2881 gcggcggggg taatcaaagc ctaaggtcgc caatgcgtcc gccacacgga agctgcccgt
     2941 atttttgccg taattcgttt caatcccgat aatcgccacg ataagctcgg caggcacgcc
     3001 gtatttttgc gccacatcat cgataaccgc gcggttttcc gcataaaacc tgcgcgcgcc
     3061 gtgaaatttc gccctgcccg aatttcccgt gcggaacaca taccacggac gcgatgtgga
     3121 gggtcggtgc ataatcttga cgatgtccgc cttgtaagcc gctttgtcaa aaaaatcctg
     3181 ccattccgcc tgggaaaaat cccctttccc gacttcatcg tccacaaaac ggcggacatt
     3241 tgcattggcg gcaaacccgc tgtcggatac cggtacggct gccgcgtcaa acgcggggcg
     3301 gctttctttt ttcatttcat ccgcgcgggg ggcttgggct tcatttgccc ggggtgtgcg
     3361 ggcctccatc gccgtacagg cagacaaagc cgccaaacaa attgccagcg gcagtatttt
     3421 tctcttttcc ataaacacat tccgaacaaa tagggtaagt gggaaagcgg cacaagggtg
     3481 aggcgcgaaa tgcggtatcc gccgcaatcg gcggttttgc agaatgccgc gcgttgcctt
     3541 ttgcgccgcc cgaaatctgc ctgccgccgt atttgcccgc tatccgcaat atcggcagtc
     3601 caatatatct ttgcggatgt cgttcagcag gaaggctgtg gtgtcttcgt gttcctcctg
     3661 tacgaagaca aagagtcggg cgatgatttt gccgatgaag actttttgca gggcgcaggc
     3721 gtcggtcagg gtgtgttcct gcaggaagct gcggatgtct tcgggcaaag cgtagtcgcg
     3781 ggcaacggcg gcaaagcgga catcggtttg caggcggtcg agcagggttt ggtttttgtc
     3841 caacttaatg cctgcttctt tttcttcggc ggtggcgcgg acgaactggt cgtaaatttc
     3901 ggcataaagc atatcgttcg ggccttcaaa aatcgtgaag gggcggatat cgatggcgat
     3961 attgccggct gggtgtccgc gttcaaaacc cttcgcgccc aagagttttt gcaacatttg
     4021 cgcggcggcg taagtgtatt ccgtggcgag ggttttgacg atgttcgcct ccatcaattg
     4081 atgggcgacg ggcgcgacgg gcgaaacgga atggcagacg tagcggtaaa ggatttcgga
     4141 aacctgatgg cggcgttgga tttcgcggcg ttcgtaatcg acgaatctga tatcgttgcg
     4201 gacatatcgg ttcaggttgt caaggatgta ttccataatg ccgtgcgtca tgccgatcag
     4261 ttgcaggcgg ctgcggataa agatgttttg gaacgcgcgc aaaccggcag cgtcgccccg
     4321 ggagagtttc atcacggcgg ttgcaggcat ttcggcatcg atgcggttga cggcgtaacg
     4381 gacggcgcgc aggccttcgg atgcgagggt ttcgcagcgg atgtatgttt tggggacgag
     4441 cagcaggtcg atgactttgg cgagtttgcc gtttttgcgc tctttggcgg caacgaggag
     4501 gaagtcgctt tgcgaattgc cctgccagta tttcgcggcg ttgacgtaaa tggtttgttc
     4561 gtcggtatat tcgtagcagg actgcatttc gcgtgcaatc gccgcgccgg aggtttcggg
     4621 ttcggtaacg cccaaaccgc cgccttcgcc tttgaaaatc atgtccaaac cttgtgcgac
     4681 ttgcgcttcg ccgccgaact cttgcagagg ctgcaacacc agcgcgcctt cgatgccggt
     4741 acgcagcgta acgggcacgc cgtaatgccc cgcaatcctt aggacttctt ggatttcaaa
     4801 ctgactgccc ttgcgcccgc cgtgtttttt gtcgaggaag ggcaacagca aacccgcctg
     4861 cttcaaggca agccatttgt cttcgggcag gtatcgcatc aggtcgatgc cgtctgaaaa
     4921 aatgcggcgg aatgcggatt cgatgtgctt taaaaaagca gccgtgtcca tggttgacgg
     4981 ctgcgcgctc ggttcggtgt gtatcatcgg cttcctctgt cggttcccat taatcggcgg
     5041 ccggtcaaac cgcctgccac agtttagagt taattttcta aactttacca caaagtgcac
     5101 cgggcaacaa tccgccggcc tttcagacgg catcgcgcac cctcacgtgc taaaatgacc
     5161 gtttgcatca ctgtccgccg attgccgcac tatgacctgc cccatcccca aaccccgtga
     5221 aaaatcccgt tggttcaatc tttcgcaagg ctcgctgccc ttggctttgg cgcgttattt
     5281 gccgcacaag cggctcaaag ccgtgctgac ccaagatgcg gaacaggcgt tgcgccttca
     5341 gacggcatgg cggtttttcc gtccgcacga cacggcggtg ttcctgccgg actgggaaac
     5401 gctgccttac gagcgttttt ctccgcatca ggatttggtg tcggagcggc tgtcggcgtt
     5461 gtggcagatt aaaagcggcg cggcggacgt gttgttcgta ccggttgcca cggcgatgca
     5521 gaagctgccg cccgtgccgt ttttggcggg gcgcacgttt tggctgaaaa cggggcagac
     5581 tttggatata ggccgcctga aaaccgattt ggtggatgcg ggctacaatc atgtttccca
     5641 cgttgtcgcg gcgggcgagt ttgccgtgcg cggcggcatt gtcgatttgt tcccgatggg
     5701 cagcgaaacg ccgtaccgca tcgatttgtt tgacgatgaa atcgacggca ttaaaacctt
     5761 cgataccgac acgcagcgca ccatttcccc cgtttccgaa atccgcctgc tgccggcgca
     5821 cgagttcccc accgacagcg aggcgcaaaa aatcttccgc agccgcttcc gcgaggaagt
     5881 cgacggtaat ccgaacgatg ccgccgtgta caaagccgtc agcaacggcc acttcggcgc
     5941 gggcgtggaa tattatctgc cgctgttttt tgaaaacgag ctggaaacgc tgtttgacta
     6001 tatcggcgaa gatgcgctgt ttgtctcttt gggcgatgtt catgccgagg caaaccgctt
     6061 ttggaacgac gttaaatcac gttacgccat ggcgcagggc gacgaaacct atccgccttt
     6121 gcttccacag catttgtatc tctctgccga tgtgttcgca ggccgtctga aaaactacgg
     6181 acaagttctg cctgatgttt ccggcaaggc acactccctg cccgaccttg ccgtcaaccg
     6241 ccaatccgac gatcctttgc aggcattgaa ggattttcag acggcctttg acgggcggat
     6301 tttgctgtgc gccgaaagtt tgggacggcg cgaaaccatg ctcggtttct tgcagcaaaa
     6361 cggtttgaaa gccaaacctg tgtccgactg gcagggcttc ttgtcggcgc acgagccgct
     6421 gatgattacg gtcgcgccgt tggcttacgg attcaaactg ggcggactgc aatcttcaag
     6481 ccaacagcaa actgttcccg cctccgaggg agaaggcaaa gcggttaccg accaaaccga
     6541 attttccgca tccgcaacaa accctctccc cagccctctc ccgcaggaga gggaacaaag
     6601 tgccgccgcc gtttcagacg gcctgaaagc agccgccgtt tcaaccgaaa gcagcctgta
     6661 tctcgtcgca agtgatctgc acgggcaaac ccgacagcaa tctgccccct cccccgtggg
     6721 ggagggttgg ggagagggca aagcggttgc cgctcaaagt gccatcgccg tcatcaccga
     6781 atccgatctt taccaatacg tcgcccgttc gcgcgtccac aaccgccgta agaaacacgc
     6841 cgctgtttca gacggcctgt tgcgcgacct tgccgaaatc aatatcggcg atcccgtcgt
     6901 gcacgaagaa cacggcatcg ggcggtatac gggcttggta acgatggatt taggcggcga
     6961 aaccaacgaa atgatgttgc tcgaatacgc gggcgaagcg cagctttatg tgcctgtttc
     7021 gcaactgcat ttaatcagcc gctactccgg tcaggcgcat gaaagcgtcg ccctgcacaa
     7081 gctcggcagc ggcgcgtgga acaaggcgaa gcgcaaagcc gccgaaaaag cgcgcgatac
     7141 cgccgccgag ttgctcaacc tctacgccca acgcgccgcc caatcgggac acaagtttga
     7201 aatcaacgag tcggactatc aggcgtttgc cgacggcttc ggctacgagg aaaccgaaga
     7261 ccaggccgcc gccatcgccg ccgtgattaa agatttgacg caggcgaagc cgatggaccg
     7321 acttgtgtgc ggcgatgtcg gtttcggcaa aaccgaagtc gccctgcgcg ccgcgtttgt
     7381 ggcggtgatg ggcggcaaac aggtcgccgt actcgccccg accacgcttc tggtcgagca
     7441 gcacgcgcaa aacttcgccg accgtttcgc cgatttcccc gtaaaagtcg ccagcctttc
     7501 gcgtttcaac aacagcaaag ccaccaaagc cacgctcgaa ggcatggcgg acggtactgt
     7561 cgatatcgtt atcggtacgc acaaattggt gcaggacgac ataaaattca aaaacttagg
     7621 tttattgatt atcgatgaag aacaccgctt cggcgtgcga cagaaagagc agctcaaacg
     7681 cctgcgcgcc aatgtcgaca tcctaaccat gaccgccacg ccgattccgc gcaccctcag
     7741 catggcgctc gaaggtctgc gcgacttctc gctgattacc actgcaccga gccgccgcct
     7801 cgccgtgaaa acctttgtca aaccgttcag cgaaggcagc gtgcgcgaag ccgtgttgcg
     7861 cgaactcaaa cgcggaggac aggtattttt cctgcacaat gaagtagata cgattgagaa
     7921 tatgcgcgag cggctggaaa ccctgctgcc cgaagcccgt atcggcgtgg cgcacggaca
     7981 actgcgcgag cgcgagctgg aacaagtcat gcgcgacttt ttgcagcaaa gattcaatgt
     8041 gttgctctgt tccaccatca tcgaaaccgg catcgacatc cccaacgcca acaccatcat
     8101 catcaaccgc gccgacaaat tcgggctggc ccaactgcac cagcttcgcg gacgcgtcgg
     8161 ccgcagccac caccaagcct acgcctacct gctcacgccc gaatacatca ccaaagacgc
     8221 agaaaaacgc ctcgacgcca tcgcggcggc agacgaactc ggcgcaggtt tcaccctcgc
     8281 catgcaggat ttggaaatcc gaggcgcagg cgaaatcctc ggcgaaggac aatccggcga
     8341 aatgatgcag gtcggcttca cgctctacac cgaaatgctc aaacaggccg tgcgcgacct
     8401 caaaaaaggc cgccaacccg acctcgacgc accgttgggc attaccaccg aaatcaaact
     8461 gcacagcccg gcattactgc ctgaagacta ctgccccgac atccacgagc gcctcgtcct
     8521 ctacaaacgc ctcgccgtat gcgaaaccgt gcagaaaatc aacgccatac acgaagaact
     8581 cgtcgaccgc ttcggcctga ccgaacaacc cgtcaaaacc ctcattgaaa gccaccacct
     8641 gcggcttgcg gcaaaagaac tgggtattga tgccatcgat gccacaagtg aagccgtaac
     8701 cgttaccttc ggcaaacacc actgtatcga cccgacgggg attatcctgc tgattcagac
     8761 ggataaaaaa taccggctgg caggggcaga caaattgagg tttgcagcgg aaatggaaaa
     8821 catcgaggtc aggatcaata ccgtcaaaac cgtactaaaa accctgcaag gcaaacgcct
     8881 gcccaaagga aattgatgta acgatgccgt ctgaagaaca ccgttcagac ggcatacaat
     8941 cccccaatcc tcaccaaaag gaacaatcat gtcgattttt cacccctatt tccaacaact
     9001 gtccaccgaa ggttttgacg gagaagaaga tgtcctctgg gaagacgagc tgaccgtcaa
     9061 aggcaatctt gtcgaggtat tcctgtaggc agaaaatgaa aacagcctgc caaccgaaac
     9121 attggatgca tttgccgcct tcctgtccga attggaaaaa gccgacgatg ctgcacgcgc
     9181 cgcactgacg gaatatttga aacgggacag ccgctatatc gactttcaca ccgaagatac
     9241 ccaaacaagc cgtgatgcgg caaaatttgt ccgcactatg cagttgatct atatcagctt
     9301 atgggcaaaa aatccggctt ttgccgttat ggattatatg cccgccaata tggaaagcga
     9361 cgaaatcctt gccgtcaaac tgcatttgga cggcagcatt ttctcaatcg attgggagag
     9421 ttgagccgga ttgccaatcg gcaaaaatga aatgccgtct gaaaacagct tccctgcccg
     9481 aagccttgcc attatgaatt tgaaggaaac tccactataa tacggcattc agattttcag
     9541 acggcatcac gcccgtcaga ccgcacacaa accaaaagga aatacatgtt ccgtactata
     9601 cttggcggaa aaatccaccg cgccaccgta accgaagccg atttaaacta cgtcggcagc
     9661 attaccgtcg atcaagacct gttagacgcg gcaggcatct gccccaacga aaaagtcgcc
     9721 atcgtcaaca acaacaacgg cgaacgtttt gaaacctata ccattgcagg gaaacgcggc
     9781 agcggcgtga tttgcctgaa cggtgctgca gccaggctgg tacagaaagg cgacatcgtc
     9841 atcattatgt cttatatcca actttccgaa ccggaaatcg ccgcacacga acccaaagtc
     9901 gtcttagtgg acggaaacaa taaaatccgc gacatcatct cctacgagcc gccgcacacc
     9961 gtactgtaat cccgcaaacg gacatcgatt atggatatta aaatcaacga catcaccctc
    10021 ggcaacaatt cgcctttcgt cctattcggc ggcatcaacg ttttagaaga tttggattcc
    10081 accctccaaa cctgtgcgca ttacgtcgaa gttacccgca aactgggcat cccctatatc
    10141 tttaaagcct ctttcgacaa ggcaaaccgc tcgtctatcc attcctatcg cggcgtaggc
    10201 ttggaagaag gcttaaagat ttttgaaaaa gtcaaagcag agttcggcat ccccgtcatt
    10261 accgacgtac acgaacccca tcaatgccaa cccgtcgccg aagtgtgcga tgtcatccag
    10321 cttcccgcct ttcttgcgcg gcagaccgat ttggtggccg caatggcgga aacgggcaat
    10381 gttatcaaca tcaaaaaacc ccagttcctc agcccttcgc aaatgaaaaa catcgtggaa
    10441 aaattccgcg aagccggcaa cgggaagctg attttatgcg aacgcggcag cagcttcggc
    10501 tacgacaacc tcgttgtcga tatgctcggt ttcggcgtga tgaaacaaac ctgcggcaac
    10561 ctgccggtta ttttcgacgt tacccattcc ctgcaaaccc gcgatgccgg ttctgccgca
    10621 tccggcggtc gtcgcgcaca ggctttggat ttggcacttg caggcatggc aacccgcctt
    10681 gccggcctgt tcctcgaatc gcaccccgat ccgaaactgg caaaatgcga cggccccagc
    10741 gcgctgccgc tacacctttt agaaaatttt ttaatccgca tcaaagcatt ggacgattta
    10801 atcaaatcac aaccgatttt aacaatcgag taacacggtt tcgccttatg atgcagactt
    10861 tccgaaaaat cagcctgtat gccgcaacct tgtggctcgg tatgcagatt atggcaggtt
    10921 atatcgccgc accggtgctg ttcaaaatgc tgcccaaaat gcaggcgggc gaaattgccg
    10981 gcgtattgtt cgacatcctc tcttggagcg ggcttgccgt ttggggcacg gtactggctg
    11041 ccgcctttgc cgccctaacc cggcggcaaa ccgccctgct gcttttttta ttgtccgccc
    11101 ttgccgccaa ccaatttttg gttacacccg ttatcgaggc actgaaatac gggcatgaaa
    11161 attggctgtt gtcggttgca ggcggatcct tcggaatgtg gcacggtatt tccagcatga
    11221 ctttcatggc aaccgcccta ctttcagcag ttttaagttg gcggctttcc ggcaaagagg
    11281 ccgtctgaag ccctcccatt ttttacctcc cttcacttca cttggagaac attcatgagc
    11341 gcaatcgttg atattttcgc ccgcgaaatt ttggactcgc gcggcaaccc cacagtcgag
    11401 tgtgatgtat tgctcgaatc cggcgtaatg ggacgtgcgg ccgtaccgag cggcgcatcc
    11461 accggtcaga aagaagcttt ggaacttcgc gacggcgaca aatcccgcta ttccggcaaa
    11521 ggcgtattga aggcggtcga acacgtcaac aaccaaatcg cccaagccct catcggtatc
    11581 gatgccaacg agcaatctta tatcgaccaa atcatgatcg aattggacgg tactgaaaac
    11641 aaaggcaatt tgggtgcgaa tgcgactttg gcggtctcta tggcggttgc acgcgccgct
    11701 gccgaagact caggcctgcc gctttaccgc tacttgggcg gcgcaggtcc gatgtccctg
    11761 cccgtaccga tgatgaacgt catcaacggc ggcgaacacg ccaacaacag cctgaacatc
    11821 caagagttta tgattatgcc cgtcggcgca aaatctttcc gcgaagcgtt gcgctgcggt
    11881 gcggaaattt tccacgcctt gaaaaaactg tgcgacagta aaggcttccc gaccacagtc
    11941 ggcgacgaag gcggtttcgc ccccaacctg aacagccaca aagaagccct gcaactgatg
    12001 gtcgaagcgg ccgaagccgc cggctacaag gcgggcgaag acgtattatt cgcattggac
    12061 tgcgcgtcca gcgagttcta caaagacggc aaataccact tggaagccga aggccgctcc
    12121 tacaccaacg cggaatttgc cgaatacttg gaaggcttgg ttaacgaatt cccgattatt
    12181 tccattgaag acgggatgga cgaaaacgac tgggaaggct ggaaactgct gaccgaaaaa
    12241 ttgggcaaaa aagttcaatt ggtcggcgac gacttgttcg taaccaatcc gaaaattctt
    12301 gccgaaggca tcgaaaaagg cgtagcaaac gcattgctgg tcaaagtcaa ccaaatcggt
    12361 actttaagcg aaaccctgaa agccgtcgat ctggcaaaat gcaaccgcta cgccagcgtg
    12421 atgagccacc gctccggcga aaccgaagac agtaccattg ccgacttggc agtcgccacc
    12481 aactgtatgc agattaaaac cggttctttg agccgttccg accgcatggc gaaatacaac
    12541 caactgctgc gtatcgagga agaattggcg gaagccgcct actaccccgg caaagccgca
    12601 ttctaccaac tgggcaaata aataaaaaag tggatgtatg tttcaacaca cagggcaaca
    12661 catgaagcgt cgccctgtgt gttgacctga tttggaagag gttacacctc tcccaaataa
    12721 agtctaattt taccgccacg aaggacggac atccgagtgg tggggtttca accagtaagg
    12781 aaacgttgat gaagtgggta actgtcgttt tatccttcgc acttgtctgt tgccaataca
    12841 gcctgtggtt cggcaaaggc agcatcggac gcaacagcag tctgagagaa cagattgccg
    12901 ttcaagaaga aaaaaaccag acactcgccc tacgcaatca ttcccttgcc gccgaagtct
    12961 atgatttgga aaacggtcaa gaagccattt cggaaatcgc ccgggtagaa ctgggttata
    13021 tccaagacgg cgaaaccttt taccgactca tcaggcataa ccggtaatac cgtcaaaaag
    13081 ccgtccgaac caatgttcgg acggctttta tttcaacaaa ctgtcagaca gcccctcatc
    13141 ctcccccgcc aaaccgcaat ccagcctgac atccccctcg acgcaacagc agcacggcag
    13201 tatctcgtcc cgccccaaaa aagccaaagg cggctcccga taagtaacgc ttccctccaa
    13261 aatctttacc cggcacgatc cgcaatatcc gcttcggcac tgatattcca ccatatgccc
    13321 cgtccgttcc aagccttcca acagggtctc accctccaag agctcaaaaa ggcccttatt
    13381 cgtaccaatg cgcgccattt ccgaccaatc aaaaataaaa ggaacaataa ccgatcctac
    13441 ctgctgtttt tcccatcata caacacacaa atgccgtctg aaacatccgg cttcagacgg
    13501 cattttttca aaaaacattt acaactcaaa atcgcccaag tcatccgtat tcacttcaga
    13561 atcgatttga ccaatcaaat aagaggatat ttccacttcc tgcggcgcga cctgtacgtt
    13621 gtcggacgac agccacgcat taatccacgg aatcgggttt tgatttgcgc cttcaaatcc
    13681 ggccggcagc cccaccgcct gcatacgcag attggtaata tattcgacgt attgggataa
    13741 gatttctttg ttcaaaccga tcatcgaacc gtctttaaac aaatatgccg cccattcttt
    13801 ttcctgttcc gccgcttttt tgaagagttg gaaacattcg tcctgcaact cggcggcaat
    13861 ttccgccatt tccggatcat caacacccga acgcatcaga ttaagcatat gctgcgtgcc
    13921 ggtcaggtgc agggcttcat cgcgggcaat cagtttgatg attttggcgt tgccttccat
    13981 caactcacgc tcagcaaaag caaacgagca ggcaaatgaa acgtagaaac ggatggcttc
    14041 caacacattg acgcacatca ggcagagata gagttttttc ttcaacccgc gcaaagacac
    14101 agtaacgggt tttccgccga cattgtgcac cccttcgccc aacaggttgt aatattgggt
    14161 gtattcgatt aaatcatcat aatagcaggc aatgtcttcg gcgcgggcgg taatgtattc
    14221 gttttcgaca atatcatcaa acacgaccga cggatcattc acaatattgc ggatgatgtg
    14281 ggtatagctg cgcgagtgga tggtttcgct gaagctccac gtttcaaccc aagtttccag
    14341 ctcgggaatc gaaaccaaag gcagcaaggc aacattcgga ctgcgccctt ggatggaatc
    14401 gagcagtgtt tgatatttca gattgctgat gaaaatatgt ttttcgtgtt cgggcaggtt
    14461 ggcgtagtcg atacggtcgc gcgacacgtc gatttcttcc ggccgccaaa aaaacgacaa
    14521 ttgtttttca atcagttttt caaatacctc gtatttctgc tggtcataac gggcgacatt
    14581 taccggctga ccgaaaaaca tcggctcttt cagcgcgtcg tttttggttt tgggaaaggt
    14641 gctgtatgac atgactaggt gttcgcagga cataacgtct ctctttttaa aaattagtcg
    14701 acttttctat taatttttaa ttcaccatat aaaacatgtt gaattctatc aataaagtgc
    14761 catcctaaaa tagcaaatac taaaagaaca ctaataaata tataaaattt atattcaact
    14821 aagaacgacc gaatgacatc catacttttc cttttctgga aaataacaac ttagcctcat
    14881 atagtagtag gcactaattt aagaaaatcc ttgaagcctc ctactatcta gaagccttgg
    14941 atatatttca aaaattaacc tgttttaaat cttacaagcc ccaccagcgc agccgtcatc
    15001 ttgaatatcg gtctgcgtgt cgtccgcgcc gtcgcgggtg ttgtggtagt acagggtttt
    15061 gacgccgtat ttgtaggcag tcagcaggtc tttgagcatt tgtttcatag aaactttgtt
    15121 accttcgaat ttgcccgggt cgtaagcagt gttggcggaa atcgcctgat cgacgaattt
    15181 ttgcattacg ccgacaagtt tcaggtagcc ttcgttgccc ggaagctgcc acagggtttc
    15241 gtaggcattt ttcaaggttt caaactccgg cacgacttgt ttcaaaatgc cgtctttcga
    15301 tgctttgacc gttaccaatc cgcgcggcgg ctcgatgccg ttggttgcgt tggcgatttg
    15361 cgagctggtt tcagacggca tgagcgcggt cagagtagag ttgcgcaggc cgtatttgac
    15421 gatgtcggca cgcaggcttt cccagtcgta atgcaaaggc tcgccgcaga cggcatccaa
    15481 atcttttttg taggtgtcga tgggcagttt gccttgcgaa taaacggttt ggttaaagag
    15541 cgtgcacgct ccgtattctt tggcaaggtt tgccgatgct ttgagcaggt aatactgtat
    15601 ggcttcaaag gtacggtggg tcagaccgag cgcggaaccg tcgctgtagc ggacaccgtt
    15661 tttcgccaga tagtaggcgt agttgatgac accgatgccg agcgaacgcc ggtccatagt
    15721 agaggtacgc gcggcttcta ccggatatcc ctgataatct aaaagtgcat cgagtgcgcg
    15781 cacggtcaaa tcggcaagcc cttccaattc gtccaagctg tttaatgcac ccaagttgaa
    15841 ggcggacagt gtacacaggg ctatttcgcc gtcagggtcg ttgatgttgt ccagcggttt
    15901 ggtcggcagg gcgatttcca tacacaagtt ggactgatga acaggcgcaa cgcgcggatc
    15961 gaacgggctg tgcgtattgc agtgatcgac gttttgaatg tagatgcgcc cggtcccggc
    16021 acgctcctgc atcagcgtgg aaaacaggtc ggcagccgga atgatacgct tgcgtatgtt
    16081 cgggtcttgc tcgtatttcg tatagagccg ctcaaattcg tcttggtcgg caaaaaacgc
    16141 ttcgtacaac cccgaaacct cgttgggcga aaacagcgta atgttgccgc ccttaatcag
    16201 gcgggtgtac agcaggcggt tgatttgcac gccataatca agctggcgga tacggttgtc
    16261 ttccacgccc cggttgtttt tcaacaccag caggctttcg gcttcaatat gccacaaggg
    16321 gtagaacaag gttgccgcgc cgccgcgcac gccaccttgc gaacaagatt tgaccgccgc
    16381 ctgaaacatc ttaaagaagg gaatgcagcc ggtatgccgg gcttcaccgc cccggatttc
    16441 gctgtccagc ccgcggatac gtccggcatt gatgccgatg cccgcgcgtt gggaaacgta
    16501 tttcacaatc gcgctggtag tggcattgat ggaatccaaa ctatcgtcgc attcaatcag
    16561 cacacagctt gagaactggc gcgtaggcgt acgcacgccg ctcataatcg gcgtgggcaa
    16621 cgatactttg aatgtggaaa cggcatcgta aaaccgtttg acgtaaccca agcgcgcctc
    16681 tttcgggtat ttgctgaaaa ggcacatcgc caccaaaaca tataaaaact gcggcgtttc
    16741 gtaaatctgg cgggtaacgc ggttctgtac cagatatttg ccttcgagct gtttgacggc
    16801 ggcataggaa aaggacatat cgcgttcgtg gtcgatatag gcgttcagtt cgtcaaattc
    16861 ttcgcggctg taatcctcaa ggatatgcct gtcgtatttt ccggcatcgg tgatcttttt
    16921 aacatggttg taaaggtgcg gcggctcgta ttcgccgtag gctattttac gaagatggaa
    16981 aatcgccaaa cgtgcggcaa ggtattggta gtccggggta tcttccgaaa ttaaatctgc
    17041 agcggctttg atgatggttt cgtggatgtc gtcggtgcgg atgccgttgt agaactggat
    17101 gtgcgatttc aactcgacct gcgacacgga aacattttcc aatccttccg ccgcccaagt
    17161 aacgacacgg tgaatcttat ccaaatcaat ggcttccaat cttccgtctc gtttggttac
    17221 ttttaaatca gtcggtgtat tcatcgcttc ctcttccact cttgatattc aagacacggt
    17281 cttttcaaat aaattaaggc agacaatata gtggattttt agcattttct ccagtcttga
    17341 caacgattgc atttttcagt ttcaggcaat gcgcgataaa acccgcctgt cgtatttttc
    17401 ctataacatt tgttttatct gataattctt taccgataaa aaacgggtaa attttttgcc
    17461 ttttgaccgg ctccggctac aaggcgatga aataaggatt ttccgacgaa aaaggaaaac
    17521 ttccggtttt ccgcccctgc gaattgttaa attttgcaaa gtatgatttt tgccacgccg
    17581 ccgccgacaa attccatttt cttaccgatt ggaatttatt attgagatta atatgttatt
    17641 tgaatttgca tatcaaacgg caagttttgt cggctgaatg agtctgaaac tgccgacagt
    17701 ttgcccgttc catccttcca tcctatactg gaaggaatta caaactgaaa ggatcgtcat
    17761 gtccgtcaat aaagatgctt gccatactgt tgtctgcaat actgggactg gtatcaacaa
    17821 ctgccgctgc cggtacgtca gaacccgccc accgacatac caaacatatc agcaaggcaa
    17881 acaagcagat gctgcacccc gaatgcagga aatatttgga acgccgtgcc gcgtggtacc
    17941 gatcgcaagg caacgtgcag gaattgcgcg aaaacaaaaa ggcgcgcaaa gcattccgca
    18001 ccctgcctta tgcggaacag aaaatccaat gccgggcggc ttatgaggct ttcgatgatt
    18061 tcgacggcgg caggttccgc cgttaatccc atataaaata tgctgtctga acacaagtta
    18121 agatggcatt ttccatagat atagtggatt aaattta
//
LOCUS       DAXDSD010000042        18061 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXDSD010000042 DAXDSD010000000
VERSION     DAXDSD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18061
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.042"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1251
                     /locus_tag="ACFB2Q_001075"
     CDS             <1..1251
                     /locus_tag="ACFB2Q_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFC0918114.1"
                     /translation="KYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVASAEG
                     SVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVVVGSEAKVNAERGIAIGKEAKAGG
                     KTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAAGDFS
                     TAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGNHSVS
                     LGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGVNDFD
                     AVNVRQLKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVGFGTY
                     KGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSDKVLM
                     EQLMQDNRQLKARLEKLEARLNAM"
     gene            complement(1390..1905)
                     /gene="coaD"
                     /locus_tag="ACFB2Q_001076"
     CDS             complement(1390..1905)
                     /gene="coaD"
                     /locus_tag="ACFB2Q_001076"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC0918115.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(2035..2718)
                     /locus_tag="ACFB2Q_001077"
     CDS             complement(2035..2718)
                     /locus_tag="ACFB2Q_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFC0918116.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            complement(2875..3141)
                     /locus_tag="ACFB2Q_001078"
     CDS             complement(2875..3141)
                     /locus_tag="ACFB2Q_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC0918117.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(3215..3685)
                     /gene="rlmH"
                     /locus_tag="ACFB2Q_001079"
     CDS             complement(3215..3685)
                     /gene="rlmH"
                     /locus_tag="ACFB2Q_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC0918118.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYALKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            complement(3738..4124)
                     /gene="rsfS"
                     /locus_tag="ACFB2Q_001080"
     CDS             complement(3738..4124)
                     /gene="rsfS"
                     /locus_tag="ACFB2Q_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC0918119.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            complement(4183..4788)
                     /gene="nadD"
                     /locus_tag="ACFB2Q_001081"
     CDS             complement(4183..4788)
                     /gene="nadD"
                     /locus_tag="ACFB2Q_001081"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC0918120.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRRQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPAAARYIRKHGLYEK"
     gene            complement(4785..5384)
                     /locus_tag="ACFB2Q_001082"
     CDS             complement(4785..5384)
                     /locus_tag="ACFB2Q_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC0918121.1"
                     /translation="MSELSEILSYNQKFVESGGYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(5413..6954)
                     /locus_tag="ACFB2Q_001083"
     CDS             complement(5413..6954)
                     /locus_tag="ACFB2Q_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC0918122.1"
                     /translation="MDGRRWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLAWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            7345..8731
                     /locus_tag="ACFB2Q_001084"
                     /pseudo
     CDS             7345..8731
                     /locus_tag="ACFB2Q_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            8751..9269
                     /locus_tag="ACFB2Q_001085"
     CDS             8751..9269
                     /locus_tag="ACFB2Q_001085"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC0918123.1"
                     /translation="MTTHFVVTGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(9455..10372)
                     /gene="thrB"
                     /locus_tag="ACFB2Q_001086"
     CDS             complement(9455..10372)
                     /gene="thrB"
                     /locus_tag="ACFB2Q_001086"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC0918124.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELSFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(10409..11137)
                     /gene="ubiG"
                     /locus_tag="ACFB2Q_001087"
     CDS             complement(10409..11137)
                     /gene="ubiG"
                     /locus_tag="ACFB2Q_001087"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC0918125.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            11289..12530
                     /locus_tag="ACFB2Q_001088"
     CDS             11289..12530
                     /locus_tag="ACFB2Q_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC0918126.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSGGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWATGGLVADAKPSVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPVFKG"
     gene            12682..13209
                     /locus_tag="ACFB2Q_001089"
     CDS             12682..13209
                     /locus_tag="ACFB2Q_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC0918127.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQRRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIVAHSHMMRRELVGLYGVPPERIQ
                     VAPPPRRYGTLLSTT"
     gene            13223..13738
                     /locus_tag="ACFB2Q_001090"
     CDS             13223..13738
                     /locus_tag="ACFB2Q_001090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC0918128.1"
                     /translation="MRAKYGFADHETVFLFPSTGHTRKGLELLADFFEQTELPVKLAV
                     AGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESALCGTRVVLSE
                     NMACTEVMNEEAGFFFSRQNPETLAQAVARAVSLKKQGGHRLSDPMRALNYNPSLSHH
                     IDRLTDMLASV"
     gene            complement(13805..15457)
                     /locus_tag="ACFB2Q_001091"
     CDS             complement(13805..15457)
                     /locus_tag="ACFB2Q_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFC0918129.1"
                     /translation="MVAYAFLFLFVTAAVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            15600..16163
                     /gene="gmhB"
                     /locus_tag="ACFB2Q_001092"
     CDS             15600..16163
                     /gene="gmhB"
                     /locus_tag="ACFB2Q_001092"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC0918130.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            16193..16936
                     /locus_tag="ACFB2Q_001093"
     CDS             16193..16936
                     /locus_tag="ACFB2Q_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC0918131.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            16933..17622
                     /locus_tag="ACFB2Q_001094"
     CDS             16933..17622
                     /locus_tag="ACFB2Q_001094"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC0918132.1"
                     /translation="MTSLIHTLSDGIELTVEINRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPHNTANRLPEHIWFHGRQLALTAHQDTQILLMPS
                     EILVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAAASLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQWI
                     KIHGRELFALG"
     gene            17704..17835
                     /locus_tag="ACFB2Q_001095"
     CDS             17704..17835
                     /locus_tag="ACFB2Q_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFC0918133.1"
                     /translation="MPPVRGRMAASAPSAPFHFTPPFRNPPDPTCGMKRLSRREVFY"
BASE COUNT         4296 a         5460 c         4598 g         3707 t
ORIGIN      
        1 aaatatgcag tagcattagg atctagttct actgccagtg gagagtattc ctatgcgtcg
       61 ggctataatt ctgttgccag tggaaataaa tcctatgctg caggctatgc ttctgttgcc
      121 agtgcagaag gcagtgttgt aattggcgat tcaaggcaag taaaaccgga agcagatcaa
      181 ggggttgcag taggttctaa agcaacggta aagaacaaag ctaaacaaag ggttgtggta
      241 ggttctgagg caaaggtaaa tgcagaacgt ggtattgcga ttggtaaaga agccaaagca
      301 gggggcaaaa caacgaacac actgttagat ggaccggcat attatgcgga cgctattgcc
      361 gtcgggtatc aagcagaggc tggcaaaggt ggcgccatcg ctttgggaaa acaagcaaaa
      421 gcaacgaagc aaaatggaat ggcacttggc gtagaatcag aagctgccgg tgattttagt
      481 accgccgtgg gtaatgaaag caaggcaaag gggcaaggcg gcgtaggtct cggtaaccaa
      541 tctaaggcag aggcagattt tgcggtggct gtggggaata aggctgaagc gactaaggaa
      601 aactcacttg tcattggccg atatgcaagg gcaaacggaa atcattctgt gagtttagga
      661 tctcgtagtg aaataaaaga tggtgtgagc aattctgtgg caccgggtta tggttcagta
      721 gctagtgaga ataatgtcgt ctctgttgcc tataaagaaa caccacaatc tacagaactt
      781 tcctatagaa aaattgtagg tgttgatgat ggtgtcaatg attttgatgc cgtcaatgta
      841 cgccagttga aagcgatgca aggacaaaat atggctgagc tattttcagt acgcagcgaa
      901 gtgcgtggtg tggcagcgag tagtgcagcc ctatcggctc tgactccatt atcctatgat
      961 gcaaacaatc ctacgcagtt tatggtcggt ttcggcacat acaaaggacg tcaagctatg
     1021 gcattaggat tgtcccattt cgtcaatgac cgctttatgg taaaagtagg cggaacctta
     1081 ggcggcaata agacgggaca tatgatgaat gtaggcttga cttggaaatt tggaacgagc
     1141 caaggtgttc cggctacaag tgataaggtg cttatggaac agttgatgca agataatcgt
     1201 caattaaagg cacgattgga gaaattagaa gcacgtctga atgctatgta gccttgtggc
     1261 gataacggtt tattgagtat atcgccaatg ataaagtgca taattttcaa accgttgata
     1321 atatacaata aattgtaacc aatacctcaa acgggtttga acccgaatat gcctgacagg
     1381 aactttctgt caggcattgt tgttgtgatg ttctgcaatc attttttggt acacggccgg
     1441 cggcacatat cttttgacgg tttccatcca gccctcgggc ccgaccagtc ctttgatcat
     1501 agtggacgac acttcggcga tttcgcgcgg cggcatgagg aatacggtag atatttcggg
     1561 tgcgaggtcg ctgttgatat ggcgcatgga gcgttcgtat tcataatcgg aggtggaacg
     1621 gatgccgcgc acgatgaatc ccgcatctac ctcacgggcg taatgcacca aaaaccggtt
     1681 ttgaaacact tcaattctga cgtttgggaa gttgtcggta atggcgcaca acatatcctg
     1741 cctttcagcg gcggtatagg tgttgcgttt gtcgggattg atgccgatgg cgacgatgag
     1801 ttcgtcaaac atagattgcg cctgccgtat catccacaga tgccccaatg tgggcggatc
     1861 gaaactgccg gcataaacgg cgcggcgcgg tgtattcggt aacatttgat tcctcccggc
     1921 ttcatagccg gctgtgtttt tatgtgtttg catccgtatt gtatgcccaa aacaaaatgc
     1981 cgtctgaaaa taagtgcttt cagacggcat ggtttgcatg gtgtttcgcc tacatcagtc
     2041 gtcgccgttt aaggacagca ggatgttgag caggctgctg aagatgttgt aaagcgagat
     2101 aaacagtgtc agtgccgcgc tgatgtaact gtcttcgccg ccgtcgatga cggtgcgtac
     2161 ctgccacata atcattaagg aactgaacaa gacaaaaccg gcggaaatgg tcagggcgag
     2221 tgcgggaata cccaaaaaca gattggcaac cacggcgacc atcagaatga ccgcacctac
     2281 ggtcaggaag cgtccgagcg cgttcatatc gagccgggtt cggcgtgcca atgcggacat
     2341 cgttaaaaag acggcggcgg tcatcgcggc ggcaatgccg acgattttcg caccgtcggc
     2401 aatatggagt gcgtattgca gcacggggcc gatcaatacg cccataccga atgtgaatac
     2461 catcagcagg gtaacgccgg tattgctgta acggtttttt tcgatgaagt ggatcatacc
     2521 gtagaaaaac gccaatacga cggcaaatcc tatccagcgc gaaccgaagg cggcgtaaaa
     2581 attgaaaccg gcattggcgg caagtgccgc gcctgcggct gccggaatga atgaaaatcc
     2641 gagcaggcgg taggttttct gcaggacggt gtttttagaa accgtatgcg cggtgtagtc
     2701 gtaaacgtcg tgttgcatat catctgctcc tgaaagcgcg gttggaaata acgggggatt
     2761 ttaacattgc ccaatgtcaa aatttgtccg gttgcgtgaa gataaagttg tccggcgtat
     2821 tttaaaggcc gtctgaagca gtttcggaca gcctgtgttc aaaacggaaa accgttattg
     2881 cggaacgtat ccctgcacgg catccgcgcc gtcgccgaag aaatactgct ccatctgctg
     2941 agccaggtat tcgcgcgcgc gcggatcggc aaggcttaag cggttttcgt taatcagcat
     3001 ggtttggtgg cgcgtccacg ccgcccatgc ttcttgcgat acgttttcaa aaatgcgttt
     3061 gcccaattcg ttgggcagcg gcggaaattt catgccttcg gcttctttgt tgagcttgac
     3121 gcagaatacc attcgtgcca tagcggattc ctttgctgtg ttcagaaata acggggcgat
     3181 tttaaccgat tagggggtac agacaaaagc ctttttattc ccgatgataa ggatggttgt
     3241 gcaggatgga aacggcccgg tagagctgct cggtcagaag gacgcgcacc atgccgtgcg
     3301 gcagggtgag gctggacagg cgcatcatca tgcgggcttg ttgtttgagg cggtcggtca
     3361 tgccgtccgc gccgccgatg acgaagcaga cgtgttcgcc gttttgccgc cagcttttga
     3421 ggtgttccgc cagctcgacg gaggtcggtg ctttgccgcg ttcgtcaaga acgacgagga
     3481 acgcgccttg cggaatggct tcaaggatgc gtttttcttc cgccgccata ccttggacgg
     3541 cattcacgcc cgcgccgcgt ttttcgggtt tgatttcttt gagtgcgtag gcggcgtcgc
     3601 gtccgaagcg tttggcgtat tcggcgacgg cctcatcaac ccaacgcggc attttggtgc
     3661 cgactgccaa gacggtaatg ttcaatgctt tcccccttac aggaaatgcc gtatgaatat
     3721 tcagacggca tcgggaatca gtctgcagcg tgccacggct tctgcattcc ggcgtggaaa
     3781 ctcggtttct cgccgcccca gatggtgtca atgtcgtaga agtcgcgcac ggcagggagc
     3841 atgacgtgga cgacgaggtc tcctgcatca accaacgtcc attcgccgct gtctccttcg
     3901 gtactgagga tttcaaaacc ggcttctttc aaatcgacgg caacgttgtt ggccagtgct
     3961 ttgacttggc gcgtactgtc gccgctggcg ataatcattc tggcaaacag cgaagttttg
     4021 tcttgcgttt cgagaacgga aatgtctttg gctttgatgt cttcgagggc attgacggcg
     4081 accccgacca ttttttgcag gtcttgcagt tcttgttcgt tcattgtttt cctaacggga
     4141 tgttttcaga cggcattata gcctctcctt actgatttga ctttattttt catacaaacc
     4201 gtgttttcgg atataacggg cggcggcagg cgggatgccg tctgaaacac cggcggcact
     4261 caggttgcgg cggatttccg ttgacgacac attatgcatc ggggctgaca agatgcggac
     4321 gctgccgtcc tgaagggcat tgcccagcca cgcgtgcagt tcgcgcgggg tttggtgcag
     4381 gctgtcgccc tgccgcatgg cgacggcgat attggtttcg cgcacgagca actgccattt
     4441 tttccaagta tgcagcttca gcaggctgtc gctgcccatc agccaccaaa gttgcgcgga
     4501 cgggaactgc cggcggaaga tttggacggt atcaaaagta taggttgcac tttctcggac
     4561 gatgtcgcag tcgctgacgg caaaacgcgc gtcttctgcc gtcgccagtt cgaccatggc
     4621 aaggcggtcg gcggcggaag cggaggcggc gtctttgtga tacgggccgc ctgccggcag
     4681 gaaaacaacc gcgtccaacc cgatttcgtc ggcaaaggca cgggcgatat gaaaatgccc
     4741 gttgtgtatc gggtcgaaag tgccgccgaa cagtccgatt ttcttcatga tgtttccatt
     4801 ccttccgata agtccatgcc gtctgaaaca ctgccgtcca caaccagcgt cagtttgccg
     4861 gtaacgggat ggatgaccag tccgtgaacg gcgatatggc gcggcatcag cggatggttg
     4921 cggataaggt ccaccgtgtg gcgcacgctg tcttcgacgt tgtcgaaacc ggtcagccag
     4981 ccgtcgaggt cgacacctgc ataacgcagg gtttcgatac ggtcttcggg aatccggctt
     5041 tcccggacgc gcccgaggaa ttcttcggca ttcagcccct gcataccgca atcgtgatgg
     5101 gcgatgacca taatctctct gaccttcaat tcaaacacgg caaccaaaag gctccgcatc
     5161 accgaacccc acgggtgcgt aaccagcgcg ccggcatttt taatcagctt ggcatcgccg
     5221 tttttcaaac ccaacgcgtc gggcagcagc ccgataatcc gcgcatccat acaggacaaa
     5281 accgccagcc cgcgttcggg gtatttgtcg gtaaagtatt tttcatatcc tcccgactcg
     5341 acaaacttct gattatagga aaggatttcg ctcaactcgc tcattttgcc gtcctctgaa
     5401 aaagggttcg cattataacg tttccgtccg ttttccgcct tcgccgttgt ccaacagcag
     5461 gaaaatgcac accgcaaatg ccgacaacag caatgtcaac accattgccc gcgcataatt
     5521 gtcctcaccc gcacgcccca aataggcata aatcaaagtc gtcaacgtct gccattccgg
     5581 acgcgacagg aacaaggttg ccgcaaattc gcccacacac gtcgccgccg ccaaagtcag
     5641 accgcgccgc aacgccggtt tcaagagggg gaacgtgata cggcatgccg tctgaaagcc
     5701 gtttgcgccc aaacctgccg ccgccctgcc gtaatccggc ggcagtgcat cccaggccga
     5761 taaaacatct tttgccacaa acggatacgc cagcagcgca tacatcgcca gcagcagcgg
     5821 taacgaagcc gtccaccccg gataaagcag cagcacgccc gccgaaacac aaaccggcga
     5881 caccataaac ggtaaaaaca ccagtccgcg catccacgcc agccgccgcg ccgccgccgc
     5941 atacaccaca cccaaaaccg ccgccgcaaa caccgccgcc gccgaaaagc gcaaagtatt
     6001 ccacactgcc tgccacgttt cactttccat taacacacgc cgcgattcgc cggccgacca
     6061 cgctttcaca acaattgccg acaaaggaaa caggcagcac acggacaaca ccgccaccga
     6121 aaatgccagc aatacatatt cccccaccga ttgcggcggc gacggcatca cgggggaaac
     6181 cgccttatcc gaaaccgcgc gcctgccgaa ccacgcatac agcaaccctg ccgccgccgt
     6241 tacccccaac accagccaca ccagcgccga agcccccgcc atatcgagtt cgaacataac
     6301 caactggtaa atttccactt cgaccgtggc ataacggctg ccgcccaaca gcaatgccag
     6361 cccgaacccc gaaaaacaat acaggaagac aaggcacacg ccgccggcaa gccacgggcg
     6421 caaaacgggc atttcaatgt cccaaaaccg ccgccacgcc cccgcgccca acgtccgtgc
     6481 cgtctgaagc cgtgccgcag gcacttgagc aaacccctga tacgccgccc tgaccaacac
     6541 gggcaggttg aaaaacacat tgccgtacaa caacagatac ggcgtatcct gccggccgcg
     6601 ccacaacagc ccgtccgccc cgaacagagc cagcacgccc acgcccgcca ccagcgtcgg
     6661 catcacaaac ggcagcatca gcaggcgcag caccaaagcc cgccccggga acgccagccg
     6721 cgccagcacc cacgcgacag gcacgcccaa aggcagcacc agcacacagg ttgccgccgc
     6781 ctgaaacacc gtccacgcca aacgtttgag catataggca tccgacagca ccgcgcgcca
     6841 cgccaaaccg tcatacgccg ccaccgccca caaaggcgca acgaccatta ccgccaaaaa
     6901 agccgaaggc agcagggaaa aagcaccccg taccgcccaa cgccgtccat ccatcgcctt
     6961 ccccacttga aacactgatg ttgcgattgt acccaaaagc cccacatacc gtatatttca
     7021 atccgactac gtaccgtatc cgccttcctc ccgccgtctg aaatatagcg gattaacaaa
     7081 aatcaggaca aggcggcgga ccgcaggcag tacaaatagt acggaaccgg ttcgcccggt
     7141 gcttcagcac cttagggaac cgttcccttt gagctaaggc ggggcaacgc cgtaccggtt
     7201 tttgttaatc cactataaat cgttcaaata aacaggaata taacttcaga caaacaactt
     7261 accgcccgat ttgtgctatc gttttcgcac aacttaaaaa aacctgacaa ttttgtactt
     7321 ttattacaga gaaaggcttt acaaatggac ggccggacac agacgctgtc cgcgcaaacc
     7381 ctgttgggca tttcggcggc ggcaatcatc ctcattctga ttttaatcgt caaattccgc
     7441 atccgcgcgc tgctgacact ggtcatcgcc agcctgctga cggctttggc aaccggtttg
     7501 cccacaggca gcatcgtcaa cgacgtactg gtcaaaaact tcggcggcac gctcggcggc
     7561 gtggcgcttc tggtcggtct gggcgcaatg ctcggacgtt tggtagaaac atccggcggc
     7621 gcacagtcgc tggcggacgc gctgatccgg atgttcggcg aaaaacgcgc accgttcgcg
     7681 ccgggcgttg cctcgctgat tttcggcttc ccgattttct tcgatgccgg actaatcgtc
     7741 atgctgccca tcgtattcgc caccgcacgg cgcatgaaac aggacgtact gcccttcgcg
     7801 cttgcctccg tcggcgcatt tatccgtcat gcacgtcttc ctgccgcccc atccgggccc
     7861 gattgccgct tccgaatttt acggcgcgaa catcggccag gttttgattt tgggtctgcc
     7921 gaccgccttc atcacatggt atttcagcgg ctatatgctc ggcaaagtgt tggggcgcgc
     7981 catccatgtt cccgttcccg aactgctcag cggcggcacg cgagacagcg acccgccgaa
     8041 agaacctgcc aaagcaggaa cggtcgtcgc cgtcatgctg attcccatgc tgctgatttt
     8101 cctgaatacc ggcgtatcgg ccctcatcag cgaaaaactc gtgagcgcgg acgaaacctg
     8161 ggttcagacg gcaaaaatga tcggttcgac acctgtcgcc cttctgattt ccgtattggc
     8221 cgcactgttg gtcttgggac gcaaacgcgg cgaaagcggc agcacgttgg aaaaaaccgt
     8281 ggacggcgca ctcgcccccg tctgttccgt gattctgatt accggcgcgg gcggtatgtt
     8341 cggcggcgtt ttgcgcgctt ccggcatcgg caaggcactc gccgacagca tggcggattt
     8401 gggcattccc gtccttttgg gctgcttcct tgccgccttg gcactgcgta tcgcgcaagg
     8461 ttcggcaacc gtcgccctga ccaccgccgc cgcgctgatg gctcctgccg ttgccgccgc
     8521 cggctttacc gactggcagc tcgcctgtat cgtattggca acggcggcag gttcggtcgg
     8581 ttgcagccac ttcaacgact ccggcttctg gctggtcggc cgcctcttgg atatggacgt
     8641 accgaccacg ctgaaaacct ggacggccaa ccaaaccctc atcgcactca tcggctttgc
     8701 cttgtccgca ctgctgtttg ccatcgtctg acagacggaa aggatagtaa atgactacgc
     8761 attttgtcgt tacgggcgta tgcggctgcg gcaagaccac cgccgcgccg tccctgcaga
     8821 aacacctcgg tcaatgtccc tatgccgaag gcgacgagtt ccacacccaa gccaaccgcg
     8881 acaagatggg cgcgggtatt ccgctgaccg atgaagaccg ctatccgtgg ttgggcaatc
     8941 tgcgcgactg gatgacgcaa caggcgcaaa acggcgcgga ccacaccatc gtaacctgtt
     9001 ccgccctcaa acgcggctac cgcgacattc tgcgcggagc cgaaggcaaa gccgccttca
     9061 tccacctcag tccgccgcaa gacatcaacc tcgaacgcat gatgtcgcgc aaagggcact
     9121 acatgaaagc agggatgctc gattcgcaac tggaaatcct cgaggaactg ggcgaaggcg
     9181 aatacggggt caaaatcgcc aaccccggca cgcccgaagc ggtcgaagcc gatattctga
     9241 actgggttgc ctcggaaaac ctgctttgaa gcaatatgcc gtctgaagcc cggcacagga
     9301 tgggtttcag acggcataaa catcgggaac agaatgaatt acattgattt aaaatggaaa
     9361 atacccggct atcgtctcat tttcgtttta atcagccata aaaatgccgt ccgaaaccct
     9421 ttcagacggc atttctgtca aacgccggac gcactcaatc caaactcaac agcaggttgc
     9481 ggaacgcgtt cgggtctttg ataaacgtca tctcgcccgc ctgcggaaaa tgaaaatcca
     9541 acaggcgcga cacccaaaaa cggatgcagc cggcgcgttg ggcggtcggg aaatacgcct
     9601 tttccccggc actcaagggg cgcacgccct cataaccgcc gataaacgcc tttttcaacg
     9661 cctcatccaa cttattgtcc gccgtccttg cccaatcgtt gaccgcaatc gccaagtcat
     9721 acataaaatt gccccggcag gcataataga aatcgatgaa gcccgatacc tgaccgccgt
     9781 caagcaacac attgtcttta aacaggtcgg cgtggatgat gcccgaaggc agatggttgc
     9841 cgagattgtc cttcagcgca tcgatttcgg aacacagcag cgcggcatcg tcttgcgaca
     9901 ggacgggcag cagccgggcg cacgcttccg tccaccacgc atcgtaacgc gggttttcca
     9961 tttccaaagg gaaatcggcg gcggcaaggt gcattttcgc caacatcgca ccggtatgga
    10021 aacactgctc agccgtcggc agcgcggtat ccgaaccttt caggcaggca accaggcagg
    10081 ccggcttgcc cgccaaaacg gaatcgagcc ggccgtcttt gcgcgcaacc ggcgcggcaa
    10141 ccgccacgcc cttcatactc aaatgccggt taagctccag aaaaaacgac agctcttcct
    10201 gtttcaacac ttcaaacacg gtcagcacat aacgtcccga agtcgtcgtc agaaaataat
    10261 tgctgttggt aatcccctgc gcaatgccct gcagggaaac aaattccccc aaatcgtaac
    10321 cgctcaggaa gccgcgcatt tcatcatcgg aaacactggt atagacagac atatcaactt
    10381 acttgctcaa aaacgggcaa acccgccgtc agaacgccgg acggcaggca aacatataat
    10441 tcacatcggt cgaatcgcac agggcataag tttgcgacaa cacatggtaa gtcataccct
    10501 tcgtatccgc cacatccaag cccgcctgac ggcacatccg cgccagctcg gcaggcgcga
    10561 tgaatttttt ccagtcgtgc gtgcctttgg ggacgaactt caacagatat tccgccgcca
    10621 caatcagatg caggtacgat ttcgggtttt tattgatggt ggaaaaaaac accatgccgt
    10681 ccggcttgac cagcttggca caagcacgca cgatggcggc gggatcgggg acgtgttcca
    10741 tcatttccat gcacgttacc acatcgaacg agtgcggttc cgcctcggca aggtcttcca
    10801 cgcggataca ttcgtattcg atatcggcga cattgttcaa agccgcgtgc aggcgggcgg
    10861 tttccagcga ctgctccgcc atgtcgatgc ccttgacaaa cgccgcgccg cgccgcgcca
    10921 tgctttccgc aaggatgccg cctccgcagc ccacgtccaa aacccgtttg ccgcacaaat
    10981 ccgcgtgtcc gtcgatataa tcgagccgga gcggattgat gtcgtgcaag gttttgaact
    11041 cgcccgactt gtcccaccat ttgtcggcaa tccggctgaa tttggcgatt tccccctcat
    11101 cgacattata ttttttgtcg gacattttcc ctcccatctg acgaaccgcc cactccaaaa
    11161 cccaagatta tactatggaa cacaatgccg tctgaaagcc ttttcgggcg gcgcggcgca
    11221 aatgccttac aaatccttac actttacggc ataatggcgg ctcgcttttt ctggcagaaa
    11281 gacaaaatat gcccaccaaa accccttcac tgttcggcgg cgcgatgatt atcgccggca
    11341 cggtcatcgg cgcaggtatg ctcgccaacc cgaccgccac atccggcgta tggtttaccg
    11401 gctcgctggc cgtgttgctg tacacctggt tttccatgct ctccggcggc ctgatgattt
    11461 tggaagtcaa cacccattat ccgcacggcg caagtttcga cacgatggtc aaagacctgc
    11521 tcggacgcgg ctggaacatc atcaacggca tcgccgtcgc cttcgtttta tacctgctca
    11581 cctacgccta catcttcgtc ggcggcgacc tgaccgccaa aggcatcggc agcgcagtag
    11641 gcggcaaaat ttcgctcacc gtcggacaac tcgtcttctt cggcatcctc gccttttgcg
    11701 tatgggcatc cgcacgcttg gtcgaccgct ttaccggcgt cctcatcggc ggcatggtat
    11761 taacctttat ttgggcaacc ggcggcctgg ttgccgatgc caaaccgtcc gtcctcttcg
    11821 acacccaagc ccccgccggc accggctact ggatttacgc cgccaccgcc ctgcccgtct
    11881 gcctcgcttc cttcggcttc cacggcaacg tttccagcct gctcaaatac tttaaaggcg
    11941 acgcgcccaa agtggcgaaa tccatctggg caggtacgtt ggttgccttg gtaatttacg
    12001 tcctctggca aaccgccatc caaggcaacc tgccgcgcaa cgagttcgcc cccgtgattg
    12061 ccgccgaagg gcaagtctcc gtcctgattg aaaccctgtc caaattcgcc caaaccggta
    12121 atatggataa aatattgtcc ctattttcct acatggcaat cgccacctcg tttttaggcg
    12181 taaccttagg cctgttcgac tacatcgccg acatcttcaa atggaacgac agcatgtccg
    12241 gccgcaccaa aaccgccgcg ctgaccttcc tgccgcccct gatttcctgc ctgctcttcc
    12301 ccaccggctt cgttaccgcc atcggctgcg tcggcctggc ggcaaccgtc tggacaggca
    12361 tcatccccgc catgctgctc taccgttcgc gcaaaaaatt cggcgcaggc aaaacctata
    12421 aagtttacgg cggcttgtgg ctgatggttt gggtcttcct tttcggcatc gccaacatcg
    12481 ccgcacaggt attgagccaa atggaactcg tccccgtatt taaaggataa aggcaaaatg
    12541 ccgtttgaaa cccgccggcg gcttcaggcg gcattgccgc aacaaacggc aaccgtattc
    12601 cggcacacag cgcattaccc taccccctca cgcacaaatc ccgccccgac aggcgcggga
    12661 cgcaaccata aaggaacaat gatgaagctc aaaatagaca ttgcaaccaa caacttcaaa
    12721 cacggcggcg gcacggaacg ctacacattg gatttggtaa agggtctgaa cagacaaaac
    12781 atcacaccgg ccgtttatgc gacgaaattt gatcacggca ttcccgaata cgccatgatc
    12841 gaaccccatc ttgtcgatca acgccggacg ctgaaaaaac tacgctcgtt cctcttttca
    12901 agccggctcg ctcaaaccag aaaaaacagt gccgccaaac tgattgcctg ccaccacgcc
    12961 gattacgccg acctcctcat ctgcggcggc acgcacttgg gctacctgca ccatatggcg
    13021 caaaaaccga acctgctcga ccgcctcgcc atacgccgca accgcagcaa ctacgccacc
    13081 gccaaactga ttgtggcgca ttcccatatg atgcggcgcg aactggtcgg actatacggc
    13141 gttccccccg aaagaatcca agtcgccccc cccccccgca gatacggaac gcttctttcc
    13201 acaacctaga gaaactgccg ccctgcgcgc caaatacggt tttgccgacc atgaaaccgt
    13261 tttcctattc ccatcgaccg gccacacgcg caaaggcctg gaactgctcg ccgacttttt
    13321 cgaacaaacc gaactgcccg tcaagctcgc cgttgccggt tcaccgcttc cccgcccgat
    13381 gaaaaacgtc gtcggactgg gcttctgcac cgatatgccc gaactctacc gcgccgccga
    13441 ctttaccatt atggcatccc tgtacgaacc cttcgggctg gtcggtgtcg aatccgccct
    13501 atgcggcaca cgcgtcgtcc tctccgaaaa catggcatgt acagaggtca tgaacgaaga
    13561 agccggattc tttttctcac gacaaaaccc ggaaaccctc gcgcaagccg ttgcccgggc
    13621 cgtcagcctt aaaaaacagg gcggacaccg cctgtccgac ccgatgcggg cgctgaacta
    13681 caacccgtcc ctgtcgcacc acatcgaccg actgaccgac atgctggcat ccgtataaca
    13741 aaatgccgtc tgaacgggtt ttcagacggc attttcgcaa ccgttgcagc cgtgcggcaa
    13801 aaagctaacg ggcaattttc aaaacgtcgt acagatagtt gcggtaattt ccgaccaact
    13861 cccggttcgg gcagtcttcc aaccctccgc cgcacaaacc gcgcatcgcc atattcgcac
    13921 gcatgaagcc gtctttggct tccaaaccag ccgcctccat cagtacgccg cctgcaaagg
    13981 caaggtcgag gaaatcctgc cgctgtacga atccgccggc aatattgctc ctgacggcaa
    14041 actgcgttac ataatccggc tgagcgtaat cccatttctt cctgacggac acgccctcaa
    14101 acggtacctg atgatcgccg aaataaccga aaacaaaggg tttgccgcgt tcgtgcagat
    14161 aacggtcgaa actttccacc gctttgtcca aatcggcaat gcgcccgatg tagtcgttaa
    14221 gcgcggatac ggttttcgca ttcaaatcgg gcgcatccaa atcaaacaca ttgtcggtat
    14281 ccgtccgata cggcccgtgc tccttcatgg tcagcacata tacgaacatc ggctgccgca
    14341 cgttttccaa atcgggatgg cgtttttcga gaatcatccg cgcatactgc atcatttcct
    14401 cactggaaat gtgccacagg tttttgccca tcggcgcggg atagcccaaa tcttgcggct
    14461 ggaacatcag attaaagccg aaatggtcat atgctgcctt ggcattgtag ttgcccttgg
    14521 taaatggcga gagcgccacg caaaaataac cgtgttcgcg caggttgcgg acaaaaccgg
    14581 tctgcaaatg cggtacgacc gaataaaaca cgccgcttgc caacgcgccg aaatctgtgg
    14641 acggaacacc cgccaaaaac gcaaattcgg acttccacgt tgcgccgccg aaagtatgca
    14701 cgcgcaaagg tgaggaaaat acagtatctt cctgccgtcc gaacattttc aaatcgggaa
    14761 ttttggcggc ggcaaaatcg aaacagtgcg gatcgagcgt cgattccatc agggtaacga
    14821 caatatcggg cttttcatcc gacatgccgt acggccgggt ttcagacggc atctgcctgg
    14881 caaacgcttc cccgtcgccc tcgaacacgg gtacttggaa aaacaccgcc cgacaggaca
    14941 tcggcaggtt caaaaacacg tcgcgcccgt cgtccggcag cgagtccagc cacgtcttta
    15001 ccgcgccggg gtgcttggaa aaatgccgca ccgacacgaa cgccgccgca aacagaaccg
    15061 cgcccgccca acgccacggc atacccaaag aatccgcacc gctccagccg aaaaccgcat
    15121 atgccgccaa agccagcagc cccgccatac cgataaccgc ttccttataa tgaaacagcg
    15181 tttcccaatt ccgccagtcg gcaatcagca aaaagtcgga aatcaacagc ggctgtttgt
    15241 aataatggat tttcagcctg tggaacagta tcagcaccac aaaccagacc gaagcgaaat
    15301 tcaaggcgcg ctgccactgc gccgtcaaca tcagcatacc gcccgccaaa aagacaaaca
    15361 gcgccgacgc gaaaaaatac gtccagcggt agtgcgacct gacaatcagc agcaccgccg
    15421 ccgttacaaa caaaaatagg aaagcatagg caaccatccc gccccactcc tcaaaacggc
    15481 atacctgtgc cgttgcgaat tattatcgaa agcgtgcatt ataatgcagc acaggcgcat
    15541 ttccaccgat atttcagtat aatgccatcc ccgaacccgc ccaacccaaa ggaaccgtga
    15601 tgaaactcat cattctcgac cgcgacggcg tcatcaaccg ggaccgcgac gacttcgtca
    15661 aatccgttga cgagtggata cctgtcgaag gcagcatgga tgcggtggca ttcctgacgc
    15721 aggcaggctg caccgttgcc gtcgccacca accaatccgg catcggccgc aaatatttta
    15781 ccgttcaaga cctcaccgaa atgcatgcca aaatgcaccg cctcatccgt caggcgggcg
    15841 gggaaatcga cggcatctgg ttctgcccgc ataccggcgc cgacggctgc aactgccgca
    15901 agcccaaacc gggcatgatt gaagacatcc tcgaacgctt caacgcccaa gcttcggaaa
    15961 cttggctggt cggcgacagc ctgcgcgatt tgcaggcaat cgatgccgtc ggcggaaaac
    16021 ccgcgctggt tctgaccgga aaaggcaaaa aaacgctctc ccaacacgga cacgaattac
    16081 ccggacacac acaggttttc gataccctgc tcgatttctc acaatacatc atgcaggaaa
    16141 acgccgcacc gcaagccgac tgaacatacc gcatttccga caaggcaaaa ccatgctcat
    16201 catccgcaac ctgatttact ggctgatact ctgttccagc ctgattttcc tctttccctt
    16261 tatgctgctc gcctcgcctt tccgggacgg ggcgcacaag atggcgcggg tctgggtcgg
    16321 catcctcaac tggtcgctca aacacatcgt cgggctcaaa taccgcatca tcggcgcgga
    16381 acacattccg gaccgcccct ccgtcatctg cgccaaacac caaagcggct gggaaacgct
    16441 cgcgctccaa gagatttttc cgccgcaggt ttacgttgcc aagcgcgagt tgttcaaaat
    16501 cccctttttc ggctggggct tgaaactggt caaaaccata ggcatagacc gcaacaaccg
    16561 ccgcgaagcc aacgaacagc tcataaaaca gggtttggcg cgcaaaaacg aaggttattg
    16621 gattaccatt ttccccgaag gcacgcgcct tgcgcccgga aaacgcggca aatacaaact
    16681 cggcggcgcg cgcatggcga aaatgtttga gatggacatc gtccccgtcg ccctcaacag
    16741 cggcgaattt tggccgaaaa attcctttct gaaatatccg ggggaaatca ccgtcatcat
    16801 ctgtccgacc atcccgcacg caagcggcag cgaagccgaa ttgatggaaa aatgcgaaca
    16861 cctcatcgaa acgcagcagc cgctcatttc cggcgcaggc ccgtttgccg ccgaaatgcc
    16921 gtctgaaacc gcatgacctc cctgattcac accctttcag acggcataga actgaccgtc
    16981 gaaatcaacc gccgtgccaa gaaaaacctg attatccgcc ctatcggcac acataccgtc
    17041 cgcatcagcg tcccaccctg cttctccgtc tccgccctca accgctggct gtatgaaaac
    17101 gaagccgtcc tgcggcggac actggcgaaa acaccgccgc acaatacggc aaaccgactg
    17161 cccgaacata tctggttcca cggcaggcag cttgccctta ccgcccatca agacacgcaa
    17221 atcctgctga tgccgtctga aatcctcgtc cccgaaggcg cgcccgaaaa acagcttgcg
    17281 ctgctgcggg actttttgga acggcaggcg cacagttacc tgattccccg tctcgaacgc
    17341 cacgcccgca ccacgcaact cttccctgcc gccgcctcgc tgacctccgc caaaaccttc
    17401 tggggcgtgt gccgcaaaac cacaggcata cgcctcaacc ggcggctggt cggcgcaccc
    17461 gaatacgttg ccgactatgt ctgcatacac gaactctgcc acctcgccca tcccgaccac
    17521 agccccgcct tttgggaact gacccgccgc ttcgccccct acacgcccga agcaaaacag
    17581 tggatcaaaa tccacggcag ggagcttttc gccttgggct gacacagccc cgggccgacg
    17641 ctttcagacg gcatccgtgc cggaacaggc acgcgcccgc ccgattcaaa ccgcgatgac
    17701 gctttgccgc cggttcgggg caggatggcg gcatccgcgc cgtccgcacc gtttcatttc
    17761 acaccgccct tccgaaaccc gcccgatccg acgtgcggta tgaaacgctt gagccggcgc
    17821 gaagtcttct actgatttgc ccgcgaaaat accgtccgaa aggttttcgg acggcatttt
    17881 tttgcgtttt cctggatggc ggcggattcg taaaaggcgg gcagggtgga ttgcaggatg
    17941 ggttgaggcc tgccaaatcc gccaactcta ccgaatacgc cgccgtcatt cccgcgcagg
    18001 cgggaatttg gttttgtcgg tgcggaaact tatcggataa aacggtttct tagattccac
    18061 g
//
LOCUS       DAXDSD010000043        17949 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXDSD010000043 DAXDSD010000000
VERSION     DAXDSD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17949
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.043"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(50..1471)
                     /locus_tag="ACFB2Q_001096"
     CDS             complement(50..1471)
                     /locus_tag="ACFB2Q_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC0918134.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
     gene            complement(1830..2189)
                     /locus_tag="ACFB2Q_001097"
     CDS             complement(1830..2189)
                     /locus_tag="ACFB2Q_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC0918135.1"
                     /translation="MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLMMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2186..2791)
                     /locus_tag="ACFB2Q_001098"
     CDS             complement(2186..2791)
                     /locus_tag="ACFB2Q_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HFC0918136.1"
                     /translation="MTIMNIDTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIVQIAPFHPYFQFEGTDSDGIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2838..3320)
                     /locus_tag="ACFB2Q_001099"
     CDS             complement(2838..3320)
                     /locus_tag="ACFB2Q_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC0918137.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3524..3621
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3832..4932
                     /gene="gcvT"
                     /locus_tag="ACFB2Q_001100"
     CDS             3832..4932
                     /gene="gcvT"
                     /locus_tag="ACFB2Q_001100"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC0918138.1"
                     /translation="MTALKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVVGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPDTEAVAFFKVLQTA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGRTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRSGQKQFD"
     gene            5079..5618
                     /locus_tag="ACFB2Q_001101"
     CDS             5079..5618
                     /locus_tag="ACFB2Q_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918139.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRPKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            5670..6056
                     /gene="gcvH"
                     /locus_tag="ACFB2Q_001102"
     CDS             5670..6056
                     /gene="gcvH"
                     /locus_tag="ACFB2Q_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC0918140.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            6209..7456
                     /gene="hemA"
                     /locus_tag="ACFB2Q_001103"
     CDS             6209..7456
                     /gene="hemA"
                     /locus_tag="ACFB2Q_001103"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC0918141.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7703..10009
                     /locus_tag="ACFB2Q_001104"
                     /pseudo
     CDS             7703..10009
                     /locus_tag="ACFB2Q_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753899.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            10138..12105
                     /gene="nosZ"
                     /locus_tag="ACFB2Q_001105"
                     /pseudo
     CDS             10138..12105
                     /gene="nosZ"
                     /locus_tag="ACFB2Q_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            12390..12545
                     /locus_tag="ACFB2Q_001106"
                     /pseudo
     CDS             12390..12545
                     /locus_tag="ACFB2Q_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            12542..13633
                     /locus_tag="ACFB2Q_001107"
                     /pseudo
     CDS             12542..13633
                     /locus_tag="ACFB2Q_001107"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            13691..14587
                     /locus_tag="ACFB2Q_001108"
     CDS             13691..14587
                     /locus_tag="ACFB2Q_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC0918142.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            14584..15414
                     /locus_tag="ACFB2Q_001109"
     CDS             14584..15414
                     /locus_tag="ACFB2Q_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC0918143.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            15411..15905
                     /locus_tag="ACFB2Q_001110"
     CDS             15411..15905
                     /locus_tag="ACFB2Q_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC0918144.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(16039..17700)
                     /locus_tag="ACFB2Q_001111"
     CDS             complement(16039..17700)
                     /locus_tag="ACFB2Q_001111"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC0918145.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT         4345 a         4636 c         4865 g         4103 t
ORIGIN      
        1 attatgccgg aatttaaaat gccgtctgaa tgttcagacg gcatagcgtt tacagtagtt
       61 tgaaaacgaa aaagacgagg gtatgtacga tgaagacggg tgtcaggaag gcgaccgacc
      121 acatcatata gccgaagaaa gtcggcatcg gtacgccgcg ctgttcggca atggccttga
      181 ccatgaagtt cggtgcgttg ccgatgtagg tcagtgcgcc catgaatacc gaacccatag
      241 aaaccgccag cagcgaatga aacaggggac ccgtcattaa ggcttgggca tcgccgcccg
      301 ccatattgaa aaacacgaga taagtgggcg cgttatccaa gaatgccgac aatatgccgc
      361 tcatccagaa atacatcgta ttaatcggat gacctgccgt atcgtgaacc agcgatacca
      421 ccccgcccag cgcgcctgcc tcgcctgctt tcagaatgct caggacgggg aagatggtga
      481 tgaagatgcc gaggaagagt ttgcccactt cggcgatggg ttcaaagttg aattcgttgc
      541 ctgcgcggac ttgtttgggc gtgattgcca tagatacggc ggtcaatgta atcaggatga
      601 catcgcggac aaggttttgc agggcgtaac ggctgccgag gatttcaaat cccgggtgtt
      661 cgggtttcca aaggccggac attagaaccg cgccgaccac gcccgaaagc aggaggaagt
      721 tccatttgcc gaagatggcg attttttcgg gtttttcctg ttgtgccggc gtatcttgtg
      781 caatgctttc ctgtttgaag aaacggttgt cgatgaaata gaaggcggtc aacaggacag
      841 cggtgctaat caggacgggg gcgaacatat gtttgaccgt ccacatgaaa tctacgcctt
      901 tgaggaagcc gaggaagagt ggggggtcgc ccaaaggggt caggccgccg ccgatgtttg
      961 caaccaggaa aatgaagaag atgacgatgt gcacgcggcg ggtacggtct tggttggctt
     1021 tcagcagcgg acgaatcatc agcattgccg cgccggtcgt tcccatgata gaggcaagtg
     1081 ccgtaccgac ggcaagcagg gcggtgttga gcttgggtgt gccgttcaag tcgccccaaa
     1141 ccaaaatgcc gcctgaaatg gtgtacaggg caagcagcag caggatgaag gggatatatt
     1201 cttcaacgag tgcgtgtgcg acggtatgga taccggcgga cgcgccaaaa accaaactga
     1261 acgggatgag gaagagcaat gtccaaaagg cggtgatttt gccgtaatgg tgatgccagg
     1321 tatgcgaaaa aaacaaggga cccaatgcga tagacagcaa aatcagggca aagggcaagc
     1381 cccacagcag gtttaggttt gcgccgtccg aatctgcggc gtaaaccgat gccgggaaaa
     1441 gcattagtga aaacaggggt aggtggcgca tcgtgtttcc tcgattcaag cactgccttg
     1501 cgcggcgcgc gggagtgata caggcttcgt gccgcccgga cataggcgga ctgtgctgcc
     1561 tgtttgtttt tgaaaaaagt tttccgattg attgtaaagt aaataatcag gataaaccag
     1621 tttcccaaac cggaaggcgg cgggaaggcg gattgccgtg cttgggaata tatgttcttt
     1681 ttgataaata attttattta aaacaaataa atatatgtct ttaataaata atctgtcgaa
     1741 aacgaaaaat aaatttattt taacatatat ttgcaatgaa acaggtttgc cccacccgtt
     1801 tgtttgccct tatccctttc agacggcatt caagattcgg gtctgcgcca catccatatg
     1861 gcgacaaggg aacaaaaaac cgatgaaacc gccccgaccc accagcgttg gggaaactgc
     1921 caaaacatca tcaggcagga tgcggtcatc atgctgatgg cgaaaatctt ggctttgcgc
     1981 ggcactgcgc cgttttgttc ccagttatga accatcgggc cgaaatagcg gtgccggtgc
     2041 agccagcggt gaaagcgcgg ggatgccttt gcccagcagg cggcggagag tagtacgaac
     2101 ggcgtggtcg gcaacagcgg caaaaaaatg ccgatgatcc ccagcagcag ggagatgccg
     2161 ccgcaggcaa ttaaaagata acggatcatt ttgaaatatt tttcttattg tgcggataag
     2221 ggcaggatgt gataccgagt tttgcccagc cttcatgtcc cattttttcc agcagggcga
     2281 tattgcgttc gaatattgcc gaagcgtcgg gaaaggcttg tgcggctttg gcaatgctgt
     2341 cttcgcggat gaggtgcagc gtcggatagg gagaacggtt ggtgtagttg ccgatgccgt
     2401 ctgaatccgt gccttcaaat tggaaatagg gatgaaacgg ggcgatttgg acgatgcctt
     2461 ccaagccgtt ttcgacaacg gcggcatcgg caatgtcgag catatcgttg aatacgtcga
     2521 aatcggggaa tagggtcggg tgaaccagca gggtggtttc cagttcggtg gcgggtgtat
     2581 tgcccagtcg ctgcagttct tcgtccaagt cttccaaaaa accgtcaagg tgtttggctt
     2641 cgctgatcgc gatgcggaca aggtttttaa cgtggggggc tttggcaaag ggacacaggt
     2701 tcagaccgat gacggctttt tccaaccatt gtccggtgtg ttcggcaaca gcatctttat
     2761 tttcggaagt atcgatattc attattgtca tgtaaatgtg tttgcagagt gcacgtgcgg
     2821 gaaaatcggg aagggcacta ttccttcagc aggtggttga gcggcaggga ggtggtgtgt
     2881 ttgatttctt ttaaaacaaa gctcgattgc gcatcctgta cgccgtggtg ggataaaagc
     2941 gtatccaaaa caaaatggga aaacgcgttc atatcggtaa aaaatgcctg aagcaggtag
     3001 tcggtctcgc cggttagggc gaagcagctc aagacttccg gccattttcg aaccgatgcg
     3061 gcaaaatctt cccgcgcgtc ttttgctttg cggatggaaa cgcggataaa tgcctgaaga
     3121 cctaagttga cggattccgg agacagcagc gcggcatatt ggcggacgat gccggcatct
     3181 tccaactgct tcagacggcg caggcacgga gaaggcgaaa gtgcgacacg ttcggacagt
     3241 tcgacattgg tcagcctgcc gttttcctgg aggacctgta agattttaat atcggttttg
     3301 tctaaagtga gttggggcat atttgcgttc cgttttaagg aattcggatt gtctgtccgt
     3361 atgtttgcgg caatccgcat agatggagac catattaaca tataaaaagt tataccgtca
     3421 tccgggacaa attttgtttt cggaaaatca tgtgaaaaca gaggcggtcg gtttgcatct
     3481 cttttaagac ggcttgccca aaccgccgat tcaagacata atcgggaaat gtgcaggaga
     3541 gtgttacacc caactacaat gtaaccaccg aaggcgcaga cacccttaaa tcgctcaggt
     3601 atcagggact gcacattgaa acaaacaatc tggagagcgg cgttagaata acgtccaccg
     3661 aaggggagaa ggccgtctga accaccattc agacaaccgc gcaaagcagt gagcagactg
     3721 gtttgccatc atgcggatac ggccgaaaat ctcaggttca aggacagata gggtcatccg
     3781 cgcacaggtg cgcgggcggc atctgaacaa aaaatccgga gaaacttgag aatgactgct
     3841 ctgaaaacca ccccgtttca tcaagcccat caagatgcag gcgcgaagcc ggtagatttt
     3901 gccggctggg aactgcccat ccattatggt tcgcaaatcg ccgaacacga agccgtgcgc
     3961 accgacgccg gtatgtttga cgtatcccat atgctcgtta ccgacgtcgt cggagcgaac
     4021 gccaaagcct ttttccgcaa attgattgcc aacgacgttg ccaaactcgc ttttgtcggc
     4081 aaagcccttt attccgcttt gctcaacgac aacggcggcg tgattgacga cttgatcgtt
     4141 taccgcacca acgaggccga aacccaatac cgcatcgtgt ccaacggtgc gacccgtgaa
     4201 aaagacacgg cacaattcca caaagtcgga caagagttcg gcgttgcctt caatccgcgc
     4261 tacgacctcg gtatgcttgc cgtacaaggt cctaaagcca ttgaaaaact cctgaccgtc
     4321 aaacccgaat gggcggatgt gattcacggc ctcaaaccgt tccaaggcgc ggatttgggc
     4381 aacgactggt ttgtcgcccg tacaggctac accggcgaag acggcgtcga agtgattctg
     4441 ccggataccg aagccgtcgc attcttcaaa gtcctgcaaa cagcaggcgt acagccttgc
     4501 ggcctcggcg cgcgcgacac cctgcgcatg gaagccggta tgaacctcta cggcaacgat
     4561 atggacgacg acaccagccc gctcgaagca ggcatgggcc ggacggttga tttgaaagac
     4621 gaaagccgcg attttgtcgg taaagccgcc ttgttggcat tgaaagaaaa aggcgttgcc
     4681 gtcaaacaag tcggtttgct gctcgaaaaa ggcggcatcc tgcgcgcgca tatggaagtg
     4741 ttgaccgaca aaggaaaagg cgaaaccacc agcggcgtat tctcgcccag cctgaaacaa
     4801 tctatcgcca tcgcccgcgt accgaaagat tttgacggcg ataccgccaa agtcctgatt
     4861 cgcggcaaag aagcggacgt gcgcgtactg aagctgccgt tcgtccgcag cggtcaaaaa
     4921 cagtttgatt aatatcggtt cagacggcat ttgtttgaat taagccgtct gaaagcaggt
     4981 tttaaggaat tgttatttcc tatgaaagca aattttggca gtttattgtt gttattgatg
     5041 ttatcggctt gcgcttcgtc gccggcgagc gcggattgat gtttttcgat acagaaggaa
     5101 aatcccatac tgcaatattt atgaaaatcg ttggcggcaa tggaaagatt gccggcgagg
     5161 tggtgataga cggggaatat gctcttgata ttgtccgcat gaatgatgat ttgagtattg
     5221 ccgtgcaaga taataagggt agaagccgtg cttccgacct ggaagcattg tcgcaagcga
     5281 aatcatttac cctaattgcg ttcagccgtt atgcgggcac agttgaagtg gataaagtat
     5341 cggctgttac aggcatttgt gcagattacc gcagcaaagg cctgaaggtt gccgttgcag
     5401 acaatatgag gcccaaacaa gaaatcgttg tattccgtgc aagcggcgta atggcaagta
     5461 ataagcgcaa gataaacgaa gcatatttgg attattctgc atcctctaaa ctgccttccg
     5521 cccaaaagca agaattggaa caagataaaa acaatatgat tgccgtccat tacggacgct
     5581 tggaagaatt aatcgcacga ggtatctgcg tacgctaagt tgtgcagatg ccgtctgaaa
     5641 ataaccccct accaatggag aatcaaacca tgagcaacaa catcccaaca gaactgaaat
     5701 acgttgccag ccatgaatgg ctgcgccttg aagaagacgg tatcattacc gtcggtatta
     5761 cccatcacgc gcaagagctg ttgggcgaca tcgtgtttgt cgagctgccc gaagtcggtg
     5821 cgaacctcgc cgccgaagaa cagtccggtg tggttgagtc tgtaaaagcc gcatccgacg
     5881 tgtatgcgcc gattgcgggc gaagtcgttg ccgtcaacga tgacttgcca ggcgcgccgg
     5941 aaaccgccaa cagcgaccct tacggcgcag gctggttctt caaaatcaaa cctgccaatc
     6001 ctgccgatta cgacggtctg ctgactgccg aacaatacgc gggcgaagtg gattaaaccg
     6061 cccgtttgtc cgacggcaac cgtcggacaa acggaaactg caccttcaga cggcatttct
     6121 gcggtcggag gtgcagtttt tttgtccgtg ttttaaggat gcagttaggc tataataacg
     6181 gtctatatcc atcttcaccg attttttcat gcaacttacc gctgtcggac tcaatcatca
     6241 aaccgcacct ttaagcatac gggaaaagct ggcgtttgcc gccgccgccc tgccagaagc
     6301 cgtccgcaat cttgcccgaa gcaatgcggc aacggaggcg gtaatccttt ctacctgcaa
     6361 ccgcaccgag ctttactgcg tcggcgattc ggaagaaatc atccgatggc ttgccgatta
     6421 ccacagtttg ccgattgaag aaatccgtcc gtatctgtac acgctggata tgcaggaaac
     6481 cgtgcgccac gccttccgcg ttgcctgcgg cttggattcg atggttttgg gcgagccgca
     6541 gattttgggg cagattaaag atgcggtgcg tgcggctcaa gaacaggaaa gtatgggggc
     6601 aaaactcaat gccctgttcc aaaaaacctt ttccgttgct aaagaagtcc gtaccgatac
     6661 cgctgtcggc gaaaattcgg tttcgatggc ttccgcgtcc gtcaagttgg cggaacagat
     6721 ttttcccgac atcggcgatt tgaacgtatt gtttatcggc gcaggcgaaa tgattgagct
     6781 ggttgccact tattttgccg ccaaaaatcc ccggctgatg acggttgcca accggacgct
     6841 ggcgcgtgca caggagttgt gcgacaagct cggtgttaac gccgaaccgt gcctgctgtc
     6901 cgatctgcct gccattctgc acgattacga cgtggtggtt tcttcaacgg cgagccagct
     6961 tccgatagtc ggcaaaggca tggtcgaacg cgcattgaaa cagcgtcaga gtatgccgtt
     7021 gttcatgctt gacttggccg tgccgcgcga tattgaagcg gaagtcggcg atttgaacga
     7081 tgcgtatctt tatacggtgg acgatatggt caacatcgtc caaagcggca aggaggcaag
     7141 gcagaaggcc gccgccgccg ccgaaacgct ggtgtccgaa aaggttgccg aatttgtcag
     7201 gcagcagcag ggcaggcaga gcgttccgct gattaaggcc ttgcgggacg agggcgagaa
     7261 agcgcgcaag caggtgttgg aaaatgcgat gaaacagctt gccaaaggcg caacggcgga
     7321 agaggttttg gaacggctgt ccgtccaact gaccaacaag ctgctgcatt cgccaactca
     7381 aaccttgaat aaggcggggg aagaagataa agatttggtt catgccgtcg cgcagattta
     7441 tcatttggac aaataacggt gcgccggaaa aggatgccgt ctgaaggggt ttcagacggc
     7501 atatttttgt gccgcctgac aacatcgtga aatcccacat tatatcgatg taattacaaa
     7561 gtattatcct atatgcggat tggttttaag atttgtaaat ttgatttgca tcaaaaatcg
     7621 ccgatagatg attcatataa tatcaatatt aaagagtatc ggtatatcgg ggatagctat
     7681 gtcctgtttt tcaatccggc gtatgtctgc gtttcgggcg cggataacgg cgttttttac
     7741 cgcctttgtc tttttgacgg cggcactgcc cgcttatgcg gagcgtctgc ctgattttct
     7801 ggcgaaaata cagccttcgg aaatttttcc gggtgcggat cgttacggca agccggaagg
     7861 caagcctatg gttgcccgcg tttacaaagg cgatgagcag ctcggtttgg tttatatcac
     7921 gaccgatgcg gtcaatacgc gcggttattc gagcaaaccg atcgatacgc tgatggcttt
     7981 ggcaaacgac ggcacgatag ccggggcgaa actggtcgat catcacgaac cgattatgct
     8041 gatcggtatc ccgcaatcgc gtgtcgataa gttcatcgac aaatatatcg gtctgaattt
     8101 tattaaaaat ccgccgaccc cgagcgtggc gccgggcgac atcatcagcg gtgcgactgt
     8161 tacactgatg gtggttaacg acagcatcca gcgttcgtac aaggtcattg ccaaccaata
     8221 ccgtctgggt tcggacaagg cccttcagac ggcatccgct tccgatgttc gggaagccgc
     8281 gcctgcgtca gaaacccgtc cgcgccgtat ggcaaatccc gacaagcagg atattttgtc
     8341 ttgggacgaa cttttgaaac aaaaggccgt cggccatctg catatcacgc tcgatcaaat
     8401 caacaaactg tttgagaaag gcggcaaggc cggcgtggcc gatcacgccg aacagggcga
     8461 tcctgacgat acctttattg atttgtatgt tgccttggtc agccagcctt ccatcggtaa
     8521 aagcctgctg ggtgaggacg gctgggcgca tctgcaaaaa cggctgaaac ccgggcagca
     8581 ggcggttttg gttgccggag agggccgtta ttcttggaaa ggttcgggct atgtgcgcgg
     8641 cggtattttc gaccgtatcg agatgattca gggggagaac agcttccgtt ttaccgatgc
     8701 ccaacacgaa cgcgtcgtcg agctgtctgc cgccgatgcg ccgcgtttta aagaagtttc
     8761 ttggtttacc atccctgaag gcgtagcgtt tgacggtgcg gagccgtggc ggctgtaatt
     8821 gatggttcag cgcgtcttga gtgtgaacga taaagccttc gttaccgccg atttggatta
     8881 cgaactgccc caagcttatt acgtcgatga tcctaaagcg ccgcccgtag aaatcagcgc
     8941 gcctgtcgaa gcagtacctg ccgcagcatc cgataccgct tcagacggca ttgccgaaga
     9001 tgcgtcagct gaaaacggtg tatccaacca attatggaaa cagatttgga aagcgaaaca
     9061 ggggcagatt gtcgtcgtcg gcattgcgct gaccatcctg cttttggtat tcctgttcca
     9121 agattggatt gtgcgttacg aaaaatggta cgaccgcttc cgctttgctt tcctaacttt
     9181 tacgctcttc tatatcggct ggtatgcaca ggcgcagctt tcggtcgtca atacgctgac
     9241 gctgttttcc gccatcctta ccgaatttca ttgggaattc ttcctgatgg atccgattgt
     9301 gttcatcctg tggctgttca cggctgcaac catgctgctg tggaacaggg ggacgttttg
     9361 cggttggttg tgtcggttcg gctcattaca ggaattgact aaccgcattg ccaaaaaact
     9421 gggtgtgaaa cagattaccg taccgcatat gctgcatacc cgtttgaacg tcataaaata
     9481 cctgatttta ttcggttttt tggcaatttc gctgtatgat ttgggtacgg cggaaaaatt
     9541 tgccgaagtc gaaccgttca aaactgcgat tatcttgaaa ttcatgtgcg attggtggtt
     9601 tgtcgccttt gccgtggcac ttttgattgc ggggctgttt atcgaacgct tcttctgccg
     9661 ttatctgtgt cctttgggtg cgggcatcgc gctgccgggc agattccgcg tgttcgactg
     9721 gctgcgccgc tacaaaatgt gcggcaaccc ctgccaaatc tgtacgcacg aatgccccgt
     9781 tcaggcaatc gcgcccgagg gcgacatcca tccgaacgaa tgtatccagt gtctgcactg
     9841 ccaagtgatg taccaccacg atacgcgctg tccgcaagtt gtggcggcaa ataagaaaaa
     9901 acaaaaacag gctgctgcca aatcagggga attggaaaat gtctctaagc agccgcagga
     9961 acaggtcgtc cgttttgtca aacccgaaac ggcgcaatct gaaaaataag catccggggg
    10021 aagcctgaag ccaaagtgtc aggcaggcag ggtttccccg gaaggataat aaaatttttt
    10081 tcccatcggc ttgccgtccg aaaaccggtt tgccgtattt tatccaagga gtgttgtatg
    10141 tcagacgaaa aattagaaca aaacggcttg agccgtcgtt cgttcttagg tacggccgcc
    10201 gcttccggtg caggtattgc cggtgcgggc ctgttgggtt tggcgggttg ctctaaagac
    10261 ggcgaacagg ctgccgctaa cgcttccggc gcggctcccg tcgccaaggc gcaaggggaa
    10321 tccaaacccg gccaactgtc ttccgaagtc ggtccgggcg aactcgatca atattacggt
    10381 ttcctttccg gcggccagtc cggcgagatg cgcctgatcg gtctgccttc tatgcgcgaa
    10441 ctgatgcgta tccccgtgtt caatatggac agcgcgaccg gttggggacg caccaatgag
    10501 agcttgaaag tcctcaacgg caatattacc gaagaaaccc gtaaattctt aaaagacggc
    10561 ggcctgcgct gctaccccaa cggcgacttg caccacccgc acctgtcgtt taccgaccaa
    10621 acttatgacg gccgctatgc ctatgccaac gacaaggcaa acaaccgcgt ctgccgcgtg
    10681 cgtttggatg tgatgaaggc cgacaaaatc atcgacatcc ctaatgattc aggtattcac
    10741 ggtctgcgtc cgcaacgtta tccgaaaacc ggttatgttt ttgccaacgg cgaacacatt
    10801 acccctgtca gcggtgtagg caaactggat gatgcgaaaa cctggaatgc agtgtacacc
    10861 gccatcgacg gcgaaaccat ggagatcgca tggcaagtat tggttgacgg taacctggac
    10921 aacggcgatg ccgactatca aggcaaatat tcttttgcca cctgctacaa ctccgagcgc
    10981 gcgctgaccg tacaaggtgc gtcttccaac gagcaggact ggtgcgtcgt tttcgacctg
    11041 aaagccatcg aagaaggcat caaagcgggc gacttcaaag aagttaacgg tgtgaaaatg
    11101 ctggacggcc gcgctgaggc caaatccaaa tacacgcgtt atatccctgt gccgaactct
    11161 cctcacggct gtaacgcaag ccctgacggt aaatacatca tgcccaacgg taaactgcct
    11221 ccaaccgtta ccgtattgga tgtcagcaaa ctggacgatt tgttcgccgg caaaatcaaa
    11281 gagcgcgatg tggtcgtagc cgaaccgcaa ctgggtctcg gcccgttaca cactgcattc
    11341 gacggtcgcg gcaatgctta tacgacattg tttatcgaca gccaaatggt gaaatggaat
    11401 attgacgatg cgatcaaagc ctacaaaggc gagaaaatcg atccgatcaa acaaaaactc
    11461 gacgttcact atcaaccggg tcacaaccat acgaccatgg gcgaaaccaa agaagccgac
    11521 ggtcaatggc tggtgtcctt gaacaaattc tccaaagacc gcttcttgaa tgccggtcct
    11581 ttgaaacccg agtgcgacca attgatcgac atctccggcg acgaaatgcg tctggtacac
    11641 gacaacccga cttttgccga accgcacgac ttgtgtttgg ttgccgcgtc caaactgaac
    11701 ccgggcaaaa cttgggaccg caaagacccg tggttctagc aggatgcttt ggaacaggcg
    11761 aaaaaagacg gtgtcgagct ggaaaaagcc gccaaagccg tacgcgaagg caacaaagta
    11821 cgcgtgtata tgacggccgt cgcgcctgct tacagtatcc cgcagtttga agtgaaccaa
    11881 ggcgacgaag taaccgttta cgttaccaac gtcgaaacca tcgaggacct gacccacggc
    11941 ttcactttgg aaggctgcgg catcgctatg gaaatcggcc cgcaagccac ttcttccgta
    12001 accttcaaag ccgtccgccc gggtgtgcac tggtactact gccaatggtt ctgccacgca
    12061 ttgcatatgg aaatgtccgg tcagatgatt gttcatccca aataatgcgg cccgacggct
    12121 gaatatggag aatggcgtct gaaggcaaca ggtcttcaga cggcatttat agccaaaccg
    12181 cttgccgtac cgtttcaggc ggtttcgcta taaaaacagg cacatttgaa tgatgcttta
    12241 ttttgttcgg ccataccgaa cgaaacagag ggacactatg cacacatccg cgctccgtat
    12301 atggctgaag gccgtgctga tacttgccgc cggcagcatt tttcagacgg catcggcagc
    12361 cgtcgtccac gtttccccgc aagacaacct tgccgaaatt tttgcccgcg cccgggcggg
    12421 cgatacgatc aaactggctt ccggcgtata tcaaaccaaa ctttatatcg acaaaccgat
    12481 tacgattgaa gggcctgccg accgttccgc aaccatcgaa ggggacaaga gcgggcgtac
    12541 catagccgta cacgcgccgg acgtaacgct ccgcaacctg accgttaccc gttccggtat
    12601 gagcctgcca gcaatggatg ccggtattta tctcgaaaaa gccgccccgc gcgccctggt
    12661 tgaacacaac aatatttttg ataattcgtt cggcgtatac cttcatggtt ctgccgatgc
    12721 gatggtgcgc gagaataaaa tcgtcggcga tgcgaccttg cgcgtgaatg agcgcggcaa
    12781 tggcgttacc gtttggaacg cgcccggcgc gcaggtcgtc ggcaacgaca tttccaaagg
    12841 gcgggacggc attttttcca ataccagcac gcacaacacc tataaaaaca accgcttcag
    12901 cgacctgcgt ttcgccgtcc actatatgta caccaacgac agcgaagtca gcggcaatat
    12961 ttgcgtgggc aacaatatgg gctatgtgct gatgttttcc gaacggctca aagtgttcga
    13021 caatatcgcc gtcggcagcc gcgattaggg catcatgctc aactatgtca actattccga
    13081 tattcacgac aatattatca acaaagcggg caagtgcgtt tttgcctaca atgccaacta
    13141 cgataaactg tccgccaatc attttgaaaa ctgccaaatc ggcatgcact ttaccgccgc
    13201 catcgaaggc gcgtccctgc acgacaattc ctttatcaac aacggaagcc aggtcaaata
    13261 tgtcagtacg cgctttctcg actggagcga gggcggacac ggcaactact ggagcgacaa
    13321 cagcccgttc gatttgaacg gcgacggctt cggagacagc gcgtaccgtc ccgacggcat
    13381 catcgaccaa atcatctggc gcgcgcccgt atcgcgcctc ttgatgaaca gtcccgcaat
    13441 cagcatcgtc aaatgggcgc aggcgcagtt tcccgccgtt ctgcccggcg gcgtggtgga
    13501 cagcaaaccg ctgatgaagc cttatgcccc caaaattcaa acccgttatc aggcgatgaa
    13561 ggacgagttg ctcaaagaag ccgaaacgcg gcagtcggaa cggggcaggg cggaaaacgg
    13621 ttctttgaac tagtctgctt cagacggcac ccggattcaa atgccgtctg aaaacacaaa
    13681 aggaacaacc atgaccacac atcatgtcga attgaggaag gtaaccaaac ggttcggggc
    13741 gcaaaaagcc gtcagccaag tcgatttggt tttgaaggca ggagaaagcg tcgggcttgc
    13801 cggacacaac ggcgcgggca agtccaccat catgaagctg atactcgggc tgattacccc
    13861 gaccgaaggc gaagtgatgc ttttgggcga acgtaccggc agcaaagcgg gggcgcggct
    13921 tcgcagccaa atcggctacc tgcccgaaac cgttgcgctg cacccttcgc tgaccggcat
    13981 cgaaacgctg gatttttatg ccaaacttaa aaaacagccg ctcacgcaga accgggggct
    14041 gcttgagcgc gtcggcattt cccagaccgc acaccgccgc gtcggcactt attctaaagg
    14101 gatgcgccaa cgccttgcct tggcacaagc cctgctgggc gagcccaaag tcctgctgtt
    14161 tgacgaaccg acaaccggcc ccgaccctgc atcacgacaa atgttttacg aagtcgtgcg
    14221 cgaactcaac gggcgcggcg cgaccgtatt gctcagtacc cacgcccttg ccgagttgga
    14281 cggacacgcc gaccgcattg tcgtgatgaa aaacggcgtt aaggttgccg acggcagtat
    14341 ggacgaattg cacgtccaaa gcgggctgcc gctgaccgtc aatatccgcc tcaatgcgcc
    14401 gcgcaccctc agcagccgct ggcagccgct ttcagacggc atttcctacc gggcgcaatg
    14461 tcaggccgaa gaacgtatgg aactgttggg cgaattgggc agcctgtccg accttgccta
    14521 tttcgacatc cacacgccga cgcttgacga aatgtacgca cggttcttga aaagggagga
    14581 cgtatgaacc ccgtttggat tattaccggc aaagaagccc gcgacagcct gcgtaaccgc
    14641 tgggtgcttg ccgccgtcct cctgcttgcc gcactcgccc tttctttggg tttcctcggc
    14701 agctcgccca ccggttcggt caaggtcgat ccgctgaccg ttaccgtcgt cagcctgtcg
    14761 agcctgtcta ttttcctgat tccgctgatt gcgatgctgc tttcttatga cgcactgatc
    14821 ggcgaaatcg aacgcggtac gatggcgttg ctgttgagtt atcccatttg gcgcaaccaa
    14881 atccttgccg gcaagtttgt cggacacctc atcatccttg cccttgccac cacggcaggt
    14941 tacggattgg caggcattac gctgcaactt gccaacggcg gtttcgacat cgccgtttgg
    15001 aaaccctttg cgctgttgat tgccgccagc gtgattttag gtgcggcatt tctgtctatg
    15061 ggctacttga ttagtgcaaa ggtcaaagag cgggggacgg cggccggtat ttccatcggc
    15121 gtatggttgt ttttcgtcgt catcttcgat atggcgcttt tgggtattct ggttgccgac
    15181 tcgaaacagg tcatcaccgc gcccgtcgtt gaaacggtgc tgctgttcaa tcccaccgac
    15241 atttaccgcc tgctcaacct gaccggttac gaaaatacgg ctatgtatgc gggtatggcg
    15301 ggtttgagcg gacaaatcgg cctgaccgtc cccgttttgc tgaccgcgca ggttttatgg
    15361 gttatcattc cgcttgtttt ggcagccgga atttttagaa agcgacgaat atgaagaaaa
    15421 ccctgttggc aattgttgcc gttttcgcct taagtgcctg ccggcaggcg gaagaggcac
    15481 cgccgccttt accccggcag attagcgacc gttcggtcgg acactattgc agtatgaacc
    15541 tgaccgaaca caacggtccc aaagcccaga tttttttgaa cggcaaaccc gatcagcccg
    15601 tttggttctc caccgtcaag cagatgttcg gctataccaa gctgcccgaa gagcccaaag
    15661 gcatccgcgt gatttacgtt accgatatgg gcaatgttac cgattggacg aatcctaatg
    15721 ccgacacgga gtggatagat gcgaaaaaag ccttttacgt catcgacagc ggctttatcg
    15781 gcggtatggg cgcggaagac gcgctgccgt tcggcaacaa ggagcaggct gaaaaatttg
    15841 caaaggataa aggcggcaag gtcgtcggtt ttgacgatat gcccgatgct tacattttca
    15901 agtaaatcca aacagaaatg ccgtctgaac gttgaagcca aggttcagac ggcatttttt
    15961 gtcataggtt caaacggggc aggttcgaac aactgcccca ttccttattc ctgaccgggc
    16021 gtaacggcgg aatgaggttt acatcccgcc gtaattcggt ccgcttgcgc cttcggggca
    16081 aatccaagtg atgttttgcg tcgggtcttt gatgtcgcag gttttgcagt gcacgcagtt
    16141 tgccgcgttg atttgcaggc gcggactgcc gttttcttcg atgatttcat atacgccggc
    16201 cggacaatag cgcgtttcgg gagaggcgta ttctttatag ttcacgtcta tcatcgtttg
    16261 cggattgttc agcaccaaat ggtcgggctg gttttcttcg tgcgcgagat tggcaaggaa
    16321 gacgctgctc aagcggtcaa aggtcaacac accgtcgggt ttcggataat caatcggctt
    16381 gcacacggcg gcttttttga gctgctcgtt gtctttgccg tgatgtttca aggtccacgg
    16441 ggctttgcct ctgaaaatca tctggtcgat gccggtataa agcgagccga ggtaaacgcc
    16501 ccatttgaat gacggacgga cattgcgcgc ggcataaagc tcttgataca gccagctttg
    16561 ttcaaacagt ttttgatagt ttcccgcttc tttgccgctg tcgaagcttt ccacttcttc
    16621 aaagttttcc aacaagggga acacggcttc ggcggcgagc atggcggatt tcatcgcggt
    16681 atgaatgcct ttgatgcgcg gcatattgag gaaccccgcc gcatcgccga ttaaaacgcc
    16741 ccccttgaac gagagcttcg gcaggctttg caaaccgcct tcaatcagcg aacgcgcgcc
    16801 gtaagcaatg cggcggccgc cttcaaaggt tttgcggatt tcgggatggg ttttgaaacg
    16861 ttggaactct tcaaacggcg acagatacgg gttttgatag tccaaaccga ccacgaagcc
    16921 gacggcgact ttgttgtcgt cgaaatggta aataaacgca ccgccgtagg ttttgctgtc
    16981 cagcggccag cctgcgctat gcaccaccaa accgggctga tgctgttcag acggcacttc
    17041 ccaaacttct ttgatgccca agccgtaagt ttgcggctgg ctgttttggt cgagttggaa
    17101 acgttcgatg atttgcttgg aaagcgaacc tcggcaacct tcggcaaaca gggtttgccg
    17161 cgcccaaagc tccatgccgg gctggaaact gtcggtcggc ccgccgtctt tgcctacacc
    17221 catattgccg gtcgcaatac ctttgaccga gccgtcttcg tgatacagca cttcggcggc
    17281 ggcaaagccc ggatagattt ccacgcccat attttccgcc tgctccgcca accagcgcac
    17341 gacttcgcct aagctgacga tgtagttgcc gtgattgtcg aaattcgggg taataggcag
    17401 attgaacgct tttttctccg tcaggaacaa caccttgtcc tgagttaccg tgcgcgtcag
    17461 cggcgcgcct ttttctttcc agtcgggaat cagctcattc agcgcaatcg gatcgataac
    17521 cgcgcccgcc agcgaatgcg cccccgcctc cgaacccttc tccgccacgc aaacgctgat
    17581 ttcgcgcccg ttctgttcgg caagctgctt gagtttgatg gcggcggaca accccgacgg
    17641 gcctgcgccg acaatcacga catcgtattg catactgtcg cgggtgatgg attctgtcat
    17701 ggcggttcct gtgtatttat tattgaattg caaatccgtg attatacaac gggaacatat
    17761 agttaccaaa tacaacaaag gtcgtctgaa aaccatattt tcggttttca gacgaccttt
    17821 gtcgaaattt caataagcac gccaccattt tacctgtccg accgcaaact ccgtctgacg
    17881 tttcggactg cgtgtgaaaa acgccttatc cccaccggca tccctccctt tcggcacgac
    17941 cgccaaaat
//
LOCUS       DAXDSD010000044        16968 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXDSD010000044 DAXDSD010000000
VERSION     DAXDSD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.044"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..186
                     /gene="rplK"
                     /locus_tag="ACFB2Q_001112"
     CDS             <1..186
                     /gene="rplK"
                     /locus_tag="ACFB2Q_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="binds directly to 23S ribosomal RNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC0918146.1"
                     /translation="AGLQKGSSNPLTNKVGKLTRAQLEEIAKTKEPDLTAADLDAAVR
                     TIAGSARSMGLDVEGVV"
     gene            186..881
                     /gene="rplA"
                     /locus_tag="ACFB2Q_001113"
     CDS             186..881
                     /gene="rplA"
                     /locus_tag="ACFB2Q_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC0918147.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGSRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            1109..1609
                     /gene="rplJ"
                     /locus_tag="ACFB2Q_001114"
     CDS             1109..1609
                     /gene="rplJ"
                     /locus_tag="ACFB2Q_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC0918148.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            1667..2038
                     /gene="rplL"
                     /locus_tag="ACFB2Q_001115"
     CDS             1667..2038
                     /gene="rplL"
                     /locus_tag="ACFB2Q_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC0918149.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEAAGAKVEIK"
     gene            2229..6407
                     /gene="rpoB"
                     /locus_tag="ACFB2Q_001116"
     CDS             2229..6407
                     /gene="rpoB"
                     /locus_tag="ACFB2Q_001116"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC0918150.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(6372..6512)
                     /locus_tag="ACFB2Q_001117"
     CDS             complement(6372..6512)
                     /locus_tag="ACFB2Q_001117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918151.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            6560..10735
                     /gene="rpoC"
                     /locus_tag="ACFB2Q_001118"
     CDS             6560..10735
                     /gene="rpoC"
                     /locus_tag="ACFB2Q_001118"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC0918152.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSALVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDMMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            11024..11437
                     /locus_tag="ACFB2Q_001119"
     CDS             11024..11437
                     /locus_tag="ACFB2Q_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918153.1"
                     /translation="MKVSQSAMNKLVIAVIASMGLTACAGGGSDSSMSVQPSVSEQLK
                     DNANVDAKDEKVIEYLKKSSLKDVPKELQAKVLKVKGDEYTGVRKQYAGKLGKGESVK
                     AMLFLDGEEPFSKEQLQKMDVYVNGKKYEGSKGGN"
     gene            11455..12084
                     /locus_tag="ACFB2Q_001120"
     CDS             11455..12084
                     /locus_tag="ACFB2Q_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding protein domain-containing
                     protein"
                     /protein_id="HFC0918154.1"
                     /translation="MSEQKIEFYGADKEQNYALLKTWVYEQPYSVVRGYFGYSRKDGN
                     PIEEDGQNPEEIPFDLYLGDIRGVATDEDKLPKAGSFQYEGRAFGGNGVLSKESLDNH
                     NGVFRYTIDFDRRKGSGSIEGMEQYGKIKLEEAAIERIPYRESGSSLGLKDRVSYFGV
                     NEGVAMLEKDNEIKKYHLGIFGEAANEVAGAVSQEHKHQAVIGFGGEKK"
     gene            12322..12564
                     /locus_tag="ACFB2Q_001121"
     CDS             12322..12564
                     /locus_tag="ACFB2Q_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918155.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            12761..13621
                     /locus_tag="ACFB2Q_001122"
                     /pseudo
     CDS             12761..13621
                     /locus_tag="ACFB2Q_001122"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
     gene            13800..14171
                     /gene="rpsL"
                     /locus_tag="ACFB2Q_001123"
     CDS             13800..14171
                     /gene="rpsL"
                     /locus_tag="ACFB2Q_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC0918156.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            14289..14759
                     /gene="rpsG"
                     /locus_tag="ACFB2Q_001124"
     CDS             14289..14759
                     /gene="rpsG"
                     /locus_tag="ACFB2Q_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC0918157.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14778..16883
                     /gene="fusA"
                     /locus_tag="ACFB2Q_001125"
     CDS             14778..16883
                     /gene="fusA"
                     /locus_tag="ACFB2Q_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC0918158.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4554 a         3752 c         4416 g         4246 t
ORIGIN      
        1 gccggtttgc aaaaaggtag ttctaatcct ctgacaaaca aggtaggtaa attgacccgt
       61 gcccagttgg aagaaattgc taaaactaaa gagcctgatt tgactgctgc tgacttggat
      121 gcggctgttc gtactatagc aggttctgct cgctcaatgg gcttggacgt ggagggtgtt
      181 gtataatggc taaagtatct aaacgcttga aagttcttcg ctcttctgtt gaagctaaca
      241 aactgtacgc aatcgacgaa gcaattgcct tggttaaaaa agctgctacc gctaaatttg
      301 acgaatctgt tgacgtatct ttcaacttgg gcgttgattc tcgtaaatct gaccaagtta
      361 tccgtggttc agtcgttctg cctaaaggta ctggtaagac aacccgtgtg gctgtattta
      421 ctcaaggtgt aaatgcagaa gctgctaaag aggctggtgc ggatgtcgtc ggtttcgaag
      481 atttggctgc tgaaatcaaa gcaggcaatc tgaactttga tgttgttatt gcttctcctg
      541 atgcaatgcg tatcgttggt caattgggta ccatcttggg ttctcgtggt ctgatgccaa
      601 accctaaaat aggtacggtt acccctaatg ttgccgaagc ggtaaaaaat gcaaaagcag
      661 gtcaagtgca gtaccgtacc gacaaggcag gtattgttca tgcaacgatt ggtcgtgctt
      721 cgttcgctga agctgatttg aaagagaact ttgatgcgtt gctggacgct atcgttaaag
      781 ccaaacctgc tgctgctaaa ggtcagtacc tgaaaaaagt tgctgtatcc agcactatgg
      841 gtttaggtgt tcgcgttgat acatcaagcg tgaataacta atctaagaaa ttttcaagca
      901 actctgtttt tgagttgctt gaatttgggc tacttaaaat taagtagatg tccaagaccg
      961 tagggatcgt aagatttaat cgtaactgcc ctacgcagac ggtagtcctg aaacacattg
     1021 caagattgct tgtaagatgt ctttttaggt taccgcgctg gtgggatatc gttttggtat
     1081 cctgtttata aacagtggga ggtagacctt gagtctcaat attgaaacca agaaagtggc
     1141 ggtcgaggaa attagcgcgg caattgctaa tgctcaaacc ctcgtagtcg ctgaatatcg
     1201 cggtatcagt gtttccagta tgactgagct tcgtgcgaat gcacgtaaag aaggcgttta
     1261 tttgcgcgtt ctgaaaaata ctctggctcg tcgcgcagtg caaggtactt catttgtaga
     1321 attggccgat caaatggttg gtccgttggt ttacgctgct tctgaagatg ctgttgctgc
     1381 tgctaaagtg ttgcaccaat tcgcgaaaaa agatgacaaa attgtcgtta aagccggttc
     1441 ttacaatggc gaagtaatga atgctgctca ggttgctgag ttggcttcta ttccgagccg
     1501 cgaagagctg ttgtccaaac tgttgttcgt tatgcaagct cctgtatcgg gctttgcgcg
     1561 cggtttggct gctttggcag agaaaaaagc cggcgaagaa gctgcttaat cgattttgtt
     1621 tctgttaatc aattattttt taatacaata tttggagtaa aatagcatgg ctattactaa
     1681 agaagacatt ttggaagcag ttggttcttt gaccgtaatg gaattgaatg acctggttaa
     1741 agcttttgaa gaaaaattcg gtgtttctgc tgctgctgtt gcagttgcag gtcctgctgg
     1801 tgccggtgct gccgatgctg aagaaaaaac cgaatttgat gtcgttttgg cttctgccgg
     1861 cgatcaaaaa gtcggcgtga ttaaagttgt ccgtgcaatt actggtttgg gtctgaaaga
     1921 agctaaagac atcgttgacg gcgcacctaa aaccattaaa gagggtgttt ctaaagctga
     1981 agccgaagac atccaaaaac aactggaagc agcaggcgct aaagtcgaaa tcaaataatt
     2041 tgatgcttct tatgaaggct ggcagttttc tgccagcctt attttgcttc ttaaaataag
     2101 cattaagtat tgtttacatt tatttgcata gtttttatca aatcattgca aataaatgta
     2161 aatatcagat tgatgcgtac cgttgttttc agacggccta ttattgaaaa ttacttttcg
     2221 gagtgtgtat gaactattcg tttaccgaga aaaaacgtat ccgtaagagt tttgcaaagc
     2281 gggaaaatgt tttggaagtt ccttttctac tggcaaccca aattgattct tatgcgaaat
     2341 ttttgcagct ggaaaatgct tttgacaaac gtaccgatga cggtctgcaa gcggcattta
     2401 attctatttt cccgattgtg agccacaacg gttatgctcg tctggagttt gtatattaca
     2461 cgctgggcga gcctttgttc gatatccctg aatgtcaatt gcgcggaatc acttatgctg
     2521 cccctctgcg cgcgcgtatc cgtttggtga ttttggataa ggaagcgtct aaaccgacgg
     2581 taaaagaagt tcgtgaaaac gaagtgtata tgggcgaaat tccgttgatg accccgagcg
     2641 gttcttttgt gattaacggc acagagcgtg tgattgtttc ccagttgcac cgttcgcccg
     2701 gtgtgttctt cgagcatgac aaaggtaaga cacactcttc cggcaaattg ttattctccg
     2761 cccgcatcat tccctaccgt ggttcatggt tggattttga atttgatccg aaagatttgc
     2821 tgtatttccg tatcgaccgc cgccgtaaaa tgccggtaac gattttgttg aaggctttgg
     2881 gctacaacaa tgagcaaatc ttggatattt tctacgacaa agaaacgttt tatttgtctt
     2941 caaacggtgt tcaaaccgat ttggtcgcag gccgtctgaa aggcgaaact gccaaggtcg
     3001 atatcttgga taaagaaggc aatgtattgg ttgccaaagg taagcgcatt actgcgaaaa
     3061 atatccgtga tattaccaat gcaggcctga cccgtttgga tgtagaacag gaaagcctgc
     3121 tgggcaaagc attggctgcc gatttgattg attcggaaac cggcgaggta ttggcttctg
     3181 ccaatgatga aattacagaa gagttgttgg ccaaatttga tattaacggc gtaaaagaaa
     3241 ttacgaccct ttacatcaat gagttggatc agggtgctta tatttccaat accttgcgca
     3301 cggatgagac tgccggccgg caggcggcgc gtgttgcgat ttaccgtatg atgcgtccgg
     3361 gcgaaccgcc caccgaagag gcggtcgagc aattgtttaa ccgcttgttc ttcagtgaag
     3421 acagctacga tttgtcccgc gtaggccgta tgaaatttaa tacgcgcaca tacgaacaaa
     3481 aactgtccga agcccaacaa aactcttggt acggccgcct gttgaacgag acgtttgccg
     3541 gtgctgccga caaaggcggt tatgtcctga gcgtcgaaga tattgtcgcc tcgattgcga
     3601 ctttggtcga gttgcgtaac ggccatggcg aagtggacga tatcgatcac ttgggcaacc
     3661 gtcgagtacg gtcggtaggc gagctgactg aaaaccaatt ccgcagcggt ttggctcgtg
     3721 tggaacgtgc cgtaaaagaa cgtttgaatc aggcggaatc agaaaacttg atgccgcacg
     3781 acttgattaa tgcgaaaccc gtttctgccg ccatcaaaga attcttcggc tccagccaat
     3841 tgagtcagtt tatggatcag accaatccct tgtctgaagt aacccataaa cgccgtgtat
     3901 ctgcattggg cccgggcggt ttgacccgcg aacgtgccgg cttcgaagtg cgggacgtgc
     3961 atccgaccca ctatggacgt gtatgtccta ttgaaacgcc tgaaggtccg aacatcggtt
     4021 tgatcaactc attgtctgtt tacgcgcgca ccaatgatta cggtttcttg gaaacgcctt
     4081 accgccgcgt tattgacggc aaagtaaccg aggaaattga ttacttgtct gccatcgaag
     4141 aaggccgcta tgtgattgca caggcgaatg ccgatttgga ttcagacggc aatctgattg
     4201 gcgatttggt tacctgtcgt gaaaaaggcg aaaccattat ggcaacgccc gaccgcgtcc
     4261 aatatatgga cgtggcaacc ggtcaggtgg tatccgttgc ggcatccctg attccgttct
     4321 tggaacacga tgacgcgaac cgcgcattga tgggtgccaa catgcaacgt caggcagtgc
     4381 cttgtctgcg tcctgaaaaa ccgatggtcg gtaccggtat cgagcgttcc gttgccgttg
     4441 actctgctac tgcaatcgtt gcccgccgag gcggcgtggt cgagtatgtc gatgccaacc
     4501 gcgttgtgat ccgtgtccat gacgacgaag cgactgccgg cgaagtgggt gtcgatattt
     4561 acaatttggt taaattcacc cgttccaatc aatctaccaa catcaaccaa cgtccggctg
     4621 tcaaagcagg cgatgttttg caacgcggcg atttggtggc cgacggcgcg tccaccgatt
     4681 tgggcgaatt ggctttgggt caaaatatga ccatcgcctt catgccgtgg aacggttaca
     4741 actacgaaga ctcgattctg atttccgaaa aagtggctgc ggacgaccgc tatacttcga
     4801 ttcacattga ggaattgaat gtcgttgccc gcgataccaa gctgggtgcg gaagacatta
     4861 cccgcgatat tccgaacttg tccgagcgta tgcaaaaccg tttggacgaa tccggtatcg
     4921 tttacatcgg tgcggaagta gaagccggcg atgtgttggt aggcaaggta acgcctaaag
     4981 gcgaaaccca gctgacgccg gaagaaaaac tgctgcgcgc catcttcggc gaaaaagcgt
     5041 ctgacgtaaa agatacttca ttgcgtatgc ctaccggtat gagcggtacg gttatcgacg
     5101 ttcaagtctt tacccgcgaa ggcatccaac gcgacaaacg cgcccaatcc attatcgatt
     5161 ccgaattgaa acgctaccgt ttggatttga acgaccaatt gcgtattttc gacaacgacg
     5221 cattcgaccg tatcgagcgt atgattgtcg gtcagaaagc caacggcggt ccgatgaagc
     5281 tggccaaagg cagcgaaatc acgaccgaat atctggcggg tctgccgagc aggcacgatt
     5341 ggtttgatat ccgtttgacc gatgaagatt tggccaagca gttggaactg attaaattga
     5401 gcctgcaaca aaaacgcgaa gaagcggatg agttgtatga gatcaagaag aaaaaactga
     5461 cccaaggcga cgagctgcaa cccggcgtac aaaaaatggt gaaagtcttt atcgccatca
     5521 aacgccgtct gcaagccggt gacaaaatgg cgggccgcca cggtaataaa ggtgtggtat
     5581 ctcgcattct gcctgtggaa gacatgccgt acatggcgga cggccgtcct gtggacatcg
     5641 tactgaaccc gttgggcgta ccttcccgta tgaacatcgg tcagattttg gaagttcact
     5701 tgggttgggc ggcaaaaggt atcggcgagc gcatcgaccg tatgctgaaa gagcgacgca
     5761 aagccggcga gctgcgcgag ttcttgaaca aactctacaa cggcagcggt aagaaagaag
     5821 atttggacag cctgactgat gaagaaatca tcgaattggc ttccaatctg cgtaaaggtg
     5881 catctttcgc ctctcctgta ttcgacggtg cgaaagagtc tgaaatccgc gaaatgttga
     5941 acttggctta cccaagcgaa gatcctgagg ttgagaaact gggcttcaac gacagtaaaa
     6001 ctcaaatcac gctgtatgac ggccgttcag gcgaagcatt tgaccgcaag gttacagtcg
     6061 gtgtgatgca ctatctgaaa ctgcaccact tggttgacga aaaaatgcac gcgcgttcta
     6121 ccggcccgta cagtctggtt actcaacaac ctctgggcgg taaagctcag tttggcggcc
     6181 aacgtttcgg tgagatggag gtttgggcat tggaagcata cggcgcggca tacacgctgc
     6241 aagagatgct gactgtgaag tctgacgacg tgaacggccg taccaaaatg tacgaaaaca
     6301 tcgtcaaagg cgaacacaaa atcgatgccg gtatgcccga gtccttcaac gtattggtca
     6361 aagagattcg ctcactgggt ttggatatcg atttggaacg ctactgatac gggtttcaga
     6421 cggcataagg ggagctgttc tgcaggtatg cggggcagcc cgacaatgtt taaaaacgaa
     6481 atgccgtctg aaagcactgt acctttatcc atatcgaaaa tccgccacgc ggtaaaaata
     6541 tttccttcaa ggagcagaaa tgaatttgtt gaacttattt aatccgttgc aaaccgcagg
     6601 tatggaagaa gagtttgatg ccatcaaaat cggtattgcc tctcccgaaa ccatccgctc
     6661 atggtcttat ggcgaagtta aaaaacctga aaccatcaac taccgtacgt tcaaacccga
     6721 gcgcgacggc ttgttctgcg ccaaaatctt tggcccggtc aaagactacg aatgcttgtg
     6781 cggaaaatac aaacgattga aatttaaagg cgtaacctgt gaaaaatgtg gcgtagaagt
     6841 taccctgtcc aaagtgcgcc gcgaacgcat gggccatatc gaattggctg cacccgttgc
     6901 ccatatttgg ttcttgaaat ccctgccttc ccgcttgggt atggtattga acatgacgtt
     6961 gcgtgatatc gaacgcgtat tgtactttga agcattcgtg gtgaccgatc ccggcatgac
     7021 tccgttgcaa cgtcgtcaat tgctgactga agacgattac tacaacaaac tggacgaata
     7081 cggcgacgac ttcgatgcca aaatgggtgc ggaaggtatc cgcgaattgt tgcgaacctt
     7141 ggatgtagca ggcgaaatcg aaatcctgcg ccaagagctt gaatcgaccg gttctgacac
     7201 caaaatcaaa aaaatcgcta aacgcttgaa agtattggaa gccttccatc gttccggtat
     7261 gaagctggag tggatgatta tggatgtgct gccggtattg ccgcctgatt tgcgtccgtt
     7321 ggttccgttg gacggcggcc gttttgccac ttccgatttg aacgatttgt accgccgcgt
     7381 catcaaccgc aacaaccgtc tgaaacgcct gctggaactg catgcgcctg acatcatcgt
     7441 ccgcaacgaa aaacgtatgt tgcaagaagc ggttgattcg ctgttggata acggccgtcg
     7501 cggtaaagcc atgaccggag ccaacaaacg tccgctgaaa tcgttggcag atatgattaa
     7561 aggtaaaggc ggccgcttcc gtcaaaacct gttgggcaaa cgtgtggact actccggccg
     7621 ttccgtgatt accgtaggcc catacctgcg tctgcaccaa tgcggtttgc cgaaaaaaat
     7681 ggcgttggaa ctgttcaaac catttatttt ccacaaattg gaaaaacaag gtttggcgtc
     7741 taccgttaaa gctgcgaaaa aattggtaga acaagaagta cctgaagtat gggatatctt
     7801 ggaagaagtc atccgcgaac atccgattat gttgaaccgt gcgccgaccc tgcaccgttt
     7861 gggtattcaa gcgttcgagc ctattctgat cgaaggtaaa gcgattcagt tgcacccatt
     7921 ggtgtgtgcc gcatttaatg ccgactttga cggtgaccaa atggcagtac acgttccatt
     7981 gagcttggaa gcgcaaatgg aagcacgcac gctgatgctg gcttcaaaca acgtattgtc
     8041 tcctgccaac ggcgaaccga ttatcgtacc ttcccaagac atcgtattgg gtctgtacta
     8101 catgacccgc gaccgtatca atgccaaagg cgagggcagc ctgtttgccg atgtgaaaga
     8161 agtgcatcgt gcataccata ccaaacaggt cgagctgggt acaaaaataa ccgtacgtct
     8221 gcgcgaatgg gtgaaaaacg aagcaggtga gtttgagcct gtcgttaacc gttacgaaac
     8281 aaccgtcggc cgtgcattgt tgagcgaaat cctgccgaaa ggcctgccgt ttgaatatgt
     8341 caacaaagcg ttgaagaaaa aagaaatttc caagctgatt aacgcatcgt tccgcctgtg
     8401 cggcttgcgc gatacggtta tcttcgccga ccacctgatg tacaccggtt tcggatttgc
     8461 ggcaaaaggc ggtatttcca ttgccgttga cgatatggaa attccaaaag aaaaagcggc
     8521 cttgctggct gaagccaatg ccgaagttaa agaaatcgaa gaccaatacc gtcaaggttt
     8581 ggttaccaac ggcgaacgct acaacaaagt ggtggatatt tggggtcgtg ccggcgataa
     8641 gattgctaaa gcgatgatgg acaacctgtc caaacaaaaa gttatcgacc gtgacggcaa
     8701 cgaagtcgat caagagtcat tcaactccat ttatatgatg gcggactccg gtgcccgtgg
     8761 ttctgcggct cagattaaac agttgtccgg tatgcgcggt ttgatggcga aacctgacgg
     8821 atcgattatt gaaacgccga ttacctcaaa cttccgcgaa ggtctgaccg tattgcaata
     8881 ctttattgcg acccacggtg cgcgtaaggg tttggcggat accgcgttga aaaccgcgaa
     8941 ctccggttac ctgacccgtc gtctggtaga cgtaactcaa gacttggtcg ttgttgaaga
     9001 cgattgcggt acttcagacg gctttgtcat gaaggcagtg gtacaaggcg gtgatgtgat
     9061 tgaagcattg cgcgatcgta ttttgggtcg tgttaccgcg tctgacgttg tcgatccgtc
     9121 aagcggcgag actttggttg aagccggtac gttgttgact gaaaaactgg tggatatgat
     9181 tgaccaatcc ggtgtcgatg aagtcaaagt ccgtactccg attacttgta aaacccgcca
     9241 cggtctgtgc gcgcactgtt acggccgcga cttggcgcgc ggcaaactgg tcaatgccgg
     9301 cgaggcagtc ggcgtgattg cggctcagtc tattggtgaa ccgggtaccc agttgaccat
     9361 gcgtacgttc cacatcggtg gtgcggcatc ccgtgcggca gcagccagcc aagtcgaagc
     9421 caaatccaac ggtacggcac gtttcagcag ccaaatgcgt tatgttgcca acaacaaagg
     9481 cgagttggtt gtcatcggcc gttcttgcga agtcgtgatt cacgatgaca tcggccgtga
     9541 acgcgaacgc cacaaagtac cttacggtgc catcctgctg gtacaagacg gtatggccat
     9601 taaagccggt caaaccttgg caacttggga tccgcatacc cgtccgatga ttaccgaaca
     9661 tgcaggtatg gtgaaattcg agaacatgga agagggcgtt accgttgcaa aacaaaccga
     9721 tgatgtaacc ggtttgtccg ctttggtagt gattgacggt aaacgccgtt cctctagtgc
     9781 ttccaaactg ctgcgtccga ctgtgaaact cttggacgaa aacggcgtgg aaatctgtat
     9841 tcccggtacg tccactccgg tttcgatggc attccccgtt ggtgcggtga ttaccgtacg
     9901 cgaaggtcag gaaatcggta aaggcgacgt attggcgcgt attccgcaag cctcttccaa
     9961 aacccgcgac attaccggcg gtctgccgcg cgttgccgag ctgtttgaag cacgcgtgcc
    10021 gaaagatgcc ggtatgctgg cggaaattac cggtaccgtt tccttcggta aggagaccaa
    10081 aggcaaacaa cgtctgatta tcactgacgt ggacggtgta gcatacgaga ctttgatttc
    10141 caaagagaaa caaattctgg tacatgacgg tcaagtggta aaccgaggcg aaaccattgt
    10201 ggacggcgcg gtcgatccgc acgatattct gcgtctgcaa ggtatcgaag cactggcacg
    10261 ctacattgtc caagaggtgc aagaggttta ccgcctgcaa ggtgtgaaga tttccgataa
    10321 acacatcgaa gtcatcatcc gtcaaatgtt gcgccgtgtg aacattgcgg atgccggcga
    10381 aaccgggttt attaccggag agcaggtcga acgcggcgat atgatggcgg ccaatgaaaa
    10441 agctttggaa gaaggcaaag agccggcacg ttacgaaaac atattgctgg gtattaccaa
    10501 agcctccctg tccaccgaca gcttcatttc tgccgcatcg ttccaagaaa ccacccgtgt
    10561 gttgaccgaa gctgccatca tgggcaaaca agacgaactg cgcggtctga aagagaatgt
    10621 catcgtcggt cgcctgattc ctgccggtac cggtttgact tatcaccgca gccgtcatca
    10681 gcaatggcaa ggggtagagc aggagactgc tgaaacccaa gtaacggatg aataatcttc
    10741 ggcgcgttca ttcaataaaa aaccgcaagc cttgagcttg cggtttttct ttgtccgatt
    10801 aaggcaaaaa caagcgtttt cgtcattttg aggcgtgtgg atataagccc gataagtgtc
    10861 ccatatcttc gtgccgtcaa attatgcatc gaatagaacg agttaaatcc gaaggttatt
    10921 gtgttaaata ttgcaaaaat actttaaaaa tttcgcacgg tgggagggta gaattcccag
    10981 acttaaagct taagtttttt taacaaagtt tgggggagtc taaatgaaag tatctcaatc
    11041 agcaatgaat aaacttgtta ttgctgttat tgcttctatg gggctaacag cgtgcgcggg
    11101 tggcggttcc gattcatcta tgtcggttca accaagcgtt tcggaacaac tgaaagacaa
    11161 tgcaaatgtt gatgcgaaag atgaaaaagt tattgaatat ctgaagaaat catcattaaa
    11221 agatgtacca aaagaattgc aagccaaagt attaaaggtt aaaggtgatg aatatacagg
    11281 agtgcgaaaa caatatgcag gtaagctcgg taagggtgaa tctgtcaagg caatgctatt
    11341 tctagacggc gaagagccgt ttagtaagga acaacttcag aagatggatg tatatgttaa
    11401 tggtaaaaaa tacgaaggta gtaagggggg gaactagatg ttctccccaa aggtttgagt
    11461 gagcaaaaga ttgagtttta cggtgcggat aaagaacaga attatgcgct attaaaaact
    11521 tgggtttatg agcaaccgta ttctgtggtt agaggctatt ttggctacag tcgaaaagac
    11581 ggtaatccta tagaagagga tggacaaaat cctgaagaga ttccttttga cttgtatctg
    11641 ggcgacatta gaggcgtggc aacagatgaa gacaaattac ccaaggctgg gagctttcaa
    11701 tatgaaggtc gggcatttgg cggtaatggg gttttatcca aagaatcgtt agataaccat
    11761 aatggcgtat ttagatacac aattgatttt gatcgtagaa aaggctctgg ctcaattgaa
    11821 ggaatggaac aatatggcaa gatcaaatta gaagaagctg ctattgagag aatcccttat
    11881 cgagagtccg gctccagctt ggggttgaaa gaccgggttt cttatttcgg cgtgaacgaa
    11941 ggggtggcta tgttagagaa ggataatgag attaaaaagt atcatttggg tattttcggc
    12001 gaagcggcta atgaagtagc aggtgctgta agccaagagc ataaacatca agctgttatc
    12061 ggtttcggcg gcgagaagaa atagagaaag cactatttct atttagtgcc gtttgtccgg
    12121 gcaagatgcc gtctgaagtt tcagacggca ttttcttcaa aaaacagtcc gccaaatcga
    12181 atactgccaa gcggcaatgc aatacctcaa aacagcaata atttctttta aaacagttga
    12241 ttcttactga acataaggcc gggcaatacg aaaagaaaaa tggcgttgct gggcatttta
    12301 tttgaatttg ttatgaaatt gatgaaatct aaaaatatct tttttatggg atacctaatc
    12361 gcttcggcga cgtttgccga ggatatcggc gtgcctgtcg aaccgattaa cgtcggtagt
    12421 cgggctgcga tgccgtctga aggggaaagc ctcgccctcc tgccgtttgc agaggatgtg
    12481 ccgccggttc gcgatgcaat gccgtctgaa gttcctaaaa gcgcggcagg cggcgatgtt
    12541 cgaggcggcc gatctatacc gtgagttgtt gtcggaaaga cccgatcttg tttatccccg
    12601 ttttgatttg ggcgtgatgc tgtttgaaga caaacaatat cgtgaggcat tggttcagtt
    12661 gcaccgggcc gaggaggtgt tgccgcccga tatgcggcag cttgcacggg aatatatccg
    12721 tcaggcggaa gcggtgcagg cgtggcatcc ttcgttcaat atgaactatg aacaaacaga
    12781 taatgtaaac aatgcgtccc tttcacggga catcgtcatc aacggccgga aatggataaa
    12841 atcggaagac agcctgccca aacgcgccaa cgggataagg tatgaattgg gtattgaccg
    12901 gatgtttaac atggcaggca atcactttgc ccgcttaggc atcagcggtt ccggtgtgca
    12961 ttattggaat gcccgggatt ttagcgaaca ggcgtttcat gccgaagtag gctaccgtta
    13021 tcggaatagc cgtctagagt ggggattccg gccctttgtc aaacaaaacc ggctgggcaa
    13081 taaccgttat acggcaaata cgggtattgc gttggattac agccgccgtc tgaatgaaaa
    13141 atggcgcagc acccaatctt tccagtatgg ccgcaaacaa taccatgacg aatatttggc
    13201 aaaacgttac aacagtaaaa cgatttcggt gagcggtacg ttcagctatt acgctatgtc
    13261 ggcttggcag ttgtacgggg gtattagcgg tatgtttgat aatactgtgg agaaagagca
    13321 ggcttcacgg cgttatggtg taagtttggg tacggtgaaa atattagacg gcggtcttgg
    13381 actgaagttg ggtgcgggtt acacgaaacg gatatttaaa gcccctgcaa cattaattta
    13441 caattttact cggcgtgatg attaacatcg gatgagcgcg gctttatggc ataaaaaact
    13501 gtcgtggaaa ggatttacac cccaaataaa tttccgttac aacaagaaca acagcaatat
    13561 gcccgccttt tattcgcaca gcggcaaggg atggtttgtc agtatggaaa aaacgtattg
    13621 acagtatttt ctccagccgt ccgactgatt gtgagggatg tcggtaaata tttatcggca
    13681 aacaagaaaa tcatcttttt tcttgtcgtt atgcttgact gtctgcttgc aataaaaata
    13741 taattccact cttgccgaca tggtgtcggc aagtatttaa ctcaacagga cgagaaaata
    13801 tgccaactat caaccaattg gtacgcaaag gccgtcaaaa gcccgtgtac gtaaacaaag
    13861 tgcccgcact ggaagcctgc ccgcaaaaac gcggcgtgtg cacccgtgta tacacgacta
    13921 cccctaaaaa acctaactct gcattgcgta aagtatgtaa agtccgcctg accaacggtt
    13981 ttgaagtcat ttcatatatc ggcggtgaag gccacaacct gcaagagcac agcgtcgtac
    14041 tgattcgcgg cggccgtgta aaagacttgc cgggtgtacg ttaccacact gtacgcggtt
    14101 ctttggatac tgcaggtgtt aaagaccgca aacaagcccg ttctaaatac ggtgctaagc
    14161 gtcctaaata attaccggga ctcaaatagg cacgtcggcc gcctaagctg aacaacggcc
    14221 gagtaagtga atactcaatt gggtattcat gggaatagac ccgactgaat agattaaagg
    14281 aaattaaaat gccaagacgt agagaagtcc ccaagcgcga cgtactgcca gatcctaaat
    14341 tcggtagcgt cgagttgacc aaattcatga acgtattgat gattgacggt aaaaaatccg
    14401 ttgccgagcg tatcgtttac ggtgcgttgg aacagattga gaaaaaaacc ggcaaagcag
    14461 caatcgaagt atttaacgaa gccattgcaa actccaaacc tatcgtggaa gtgaaaagcc
    14521 gccgtgtagg tggtgcaaac taccaagttc ctgttgaagt tcgtccttca cgccgtctgg
    14581 ctttggcaat gcgttgggtt cgcgacgcgg cccgcaaacg tggtgagaaa tccatggatc
    14641 tgcgtttggc aggcgagttg attgatgcgt ccgaaggccg tggcggtgcg ttgaaaaaac
    14701 gtgaagaagt acaccgtatg gctgaagcca acaaagcatt ctctcacttc cgtttctaat
    14761 tttgaaaggc taataaaatg gctcgtaaga ccccgatcag cctgtaccgc aacatcggta
    14821 tttccgccca tatcgatgcg ggtaaaacca cgacgacaga acgtattttg ttctataccg
    14881 gtttgaccca caagctgggc gaagtgcatg acggtgcggc tactaccgac tacatggaac
    14941 aagagcaaga gcgcggtatt accattacct ccgctgccgt tacttcctac tggtccggta
    15001 tggcgaaaca attccccgag caccgcttca acatcatcga caccccgggg cacgttgact
    15061 ttaccgtaga ggtagagcgt tctatgcgtg tattggacgg cgcggtaatg gtttactgtg
    15121 cggtgggcgg tgttcaaccg caatctgaaa ccgtatggcg gcaagccaac aaataccaag
    15181 ttccgcgctt ggcgtttgtc aataaaatgg accgccaagg tgccaacttc ttccgcgttg
    15241 tcgagcaaat gaaaacccgt ttgcgcgcaa accccgtacc tatcgtcatt ccggtaggcg
    15301 cggaagacag tttcaccggt gttgtcgatt tgctgaaaat gaaatctatc atctggaatg
    15361 aagccgataa aggtacaacc tttacctatg gcgatattcc tgccgaattg gtcgaaactg
    15421 ccgaagaatg gcgtcaaaat atgattgaag ccgcagccga agccagcgaa gaactgatgg
    15481 acaaatactt gggcggtgaa gatctggccg aagaagaaat cgtaggcgcg ttgcgtcaac
    15541 gtactttggc aggcgaaatt cagcctatgc tgtgcggttc tgcatttaaa aacaaaggtg
    15601 ttcaacgtat gttggacgca gttgtagaat tgctgccagc tcctaccgat attcctccgg
    15661 ttcaaggtgt taatcctaac actgaagaag ccgacagccg tcaagccagc gatgaagaga
    15721 aattctctgc attggcattc aaaatgttga acgacaaata cgtcggtcag ctgaccttta
    15781 tccgcgttta ctcaggcgta gtaaaatccg gcgataccgt actgaattct gtaaaaggca
    15841 ctcgcgaacg tatcggtcgt ttggtgcaaa tgactgccgc agaccgtact gaaatcgaag
    15901 aagtacgcgc tggcgacatc gcagccgcta tcggtctgaa agacgttact accggtgaaa
    15961 ccttgtgtgc ggaaagcgcg ccgattatct tggaacgtat ggaattcccc gagccggtaa
    16021 tccatattgc cgttgagccg aaaaccaaag ccgaccaaga gaaaatgggt atcgccctga
    16081 accgcttggc taaagaagac ccttctttcc gcgttcgtac agacgaagaa tccggtcaaa
    16141 ccattatttc cggtatgggt gagctgcact tggaaattat tgttgaccgt atgaaacgcg
    16201 aattcggtgt ggaagcaaat atcggtgcac ctcaagtggc ttaccgtgaa actatccgca
    16261 aagccgttaa agctgaatac aaacatgcaa aacaatccgg tggtaaaggt caatacggtc
    16321 acgttgtgat tgaaatggaa cctatggaac cgggtggtga aggttacgag tttatcgatg
    16381 aaattaaagg tggtgtgatt cctcgcgaat ttattccgtc tgtcgataaa ggtatccgcg
    16441 atacgttgcc taacggtatc gttgccggct atcctgtagt tgacgtacgt atccgtctgg
    16501 tattcggttc ttaccatgat gtcgactctt cccaattggc atttgaattg gctgcttctc
    16561 aagcgtttaa agaaggtatg cgtcaagcat ctcctgccct gcttgagccg attatggcag
    16621 ttgaagtgga aactccggaa gaatacatgg gcgacgtaat gggcgacttg aaccgccgtc
    16681 gcggtgttgt attgggtatg gatgatgacg gtatcggcgg taaaaaagtc cgtgccgaag
    16741 tacctctggc agaaatgttc ggttactcga ccgacctgcg ttctgcaacc caaggccgcg
    16801 ctacttactc tatggagttc aagaaatatt ctgaagctcc tgcccacata gctgctgctg
    16861 taactgaagc ccgtaaaggc taatcaggca aataggccgt ctgaaaggct gaaatgattt
    16921 ttcagatggc attgttcttt aatcgatctt taatgtaaag gaattagc
//
LOCUS       DAXDSD010000045        16825 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXDSD010000045 DAXDSD010000000
VERSION     DAXDSD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16825
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.045"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            72..1205
                     /locus_tag="ACFB2Q_001126"
     CDS             72..1205
                     /locus_tag="ACFB2Q_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918159.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRGLSH"
     gene            complement(1708..1983)
                     /locus_tag="ACFB2Q_001127"
     CDS             complement(1708..1983)
                     /locus_tag="ACFB2Q_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC0918160.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQGEKMPSET"
     gene            complement(1980..3263)
                     /locus_tag="ACFB2Q_001128"
     CDS             complement(1980..3263)
                     /locus_tag="ACFB2Q_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC0918161.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(3412..4830)
                     /gene="glnA"
                     /locus_tag="ACFB2Q_001129"
     CDS             complement(3412..4830)
                     /gene="glnA"
                     /locus_tag="ACFB2Q_001129"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC0918162.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGPAFDGSSIGGWKGIEASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            5141..5950
                     /gene="aroE"
                     /locus_tag="ACFB2Q_001130"
     CDS             5141..5950
                     /gene="aroE"
                     /locus_tag="ACFB2Q_001130"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC0918163.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            5953..6654
                     /gene="mtgA"
                     /locus_tag="ACFB2Q_001131"
     CDS             5953..6654
                     /gene="mtgA"
                     /locus_tag="ACFB2Q_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC0918164.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(6928..7003)
                     /locus_tag="ACFB2Q_001132"
     tRNA            complement(6928..7003)
                     /locus_tag="ACFB2Q_001132"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6968..6970),aa:Phe,seq:gaa)
     gene            complement(7194..7928)
                     /gene="lptB"
                     /locus_tag="ACFB2Q_001133"
     CDS             complement(7194..7928)
                     /gene="lptB"
                     /locus_tag="ACFB2Q_001133"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC0918165.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(7999..8529)
                     /gene="lptA"
                     /locus_tag="ACFB2Q_001134"
     CDS             complement(7999..8529)
                     /gene="lptA"
                     /locus_tag="ACFB2Q_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC0918166.1"
                     /translation="MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(8510..9091)
                     /gene="lptC"
                     /locus_tag="ACFB2Q_001135"
     CDS             complement(8510..9091)
                     /gene="lptC"
                     /locus_tag="ACFB2Q_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC0918167.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKDM"
     gene            complement(9088..9624)
                     /locus_tag="ACFB2Q_001136"
     CDS             complement(9088..9624)
                     /locus_tag="ACFB2Q_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC0918168.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(9690..10664)
                     /locus_tag="ACFB2Q_001137"
     CDS             complement(9690..10664)
                     /locus_tag="ACFB2Q_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC0918169.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            10761..11816
                     /gene="tal"
                     /locus_tag="ACFB2Q_001138"
     CDS             10761..11816
                     /gene="tal"
                     /locus_tag="ACFB2Q_001138"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC0918170.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            11828..11962
                     /locus_tag="ACFB2Q_001139"
     CDS             11828..11962
                     /locus_tag="ACFB2Q_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918171.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGSALKILMGKKLMHDITRQHKRK
                     "
     gene            11967..12257
                     /locus_tag="ACFB2Q_001140"
     CDS             11967..12257
                     /locus_tag="ACFB2Q_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918172.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            12274..13161
                     /gene="gluQRS"
                     /locus_tag="ACFB2Q_001141"
     CDS             12274..13161
                     /gene="gluQRS"
                     /locus_tag="ACFB2Q_001141"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC0918173.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDSNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(13231..14247)
                     /gene="dusA"
                     /locus_tag="ACFB2Q_001142"
     CDS             complement(13231..14247)
                     /gene="dusA"
                     /locus_tag="ACFB2Q_001142"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC0918174.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            14367..15521
                     /locus_tag="ACFB2Q_001143"
     CDS             14367..15521
                     /locus_tag="ACFB2Q_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC0918175.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKMGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLRIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAMMQAQSDIKSGK"
     gene            complement(15571..15837)
                     /locus_tag="ACFB2Q_001144"
     CDS             complement(15571..15837)
                     /locus_tag="ACFB2Q_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFC0918176.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(15945..>16825)
                     /locus_tag="ACFB2Q_001145"
     CDS             complement(15945..>16825)
                     /locus_tag="ACFB2Q_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC0918177.1"
                     /translation="LRRLVAAKVRRYCGEHPGVFDGAAGSGQLEQYIEPSDFRAVEIQ
                     AEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVMNPPFSIKLKDLSEDEKSRIAQEY
                     PWKKSGVADEIFVLKGLENARRFGFFILFPGIAYRKSEQRFREIIGNRLAELNRIQNA
                     FEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLLAADTWQIEPDLWQTVQEPAPPKE
                     KEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGWPHAEFDGFCDRLCNLIQAEKYGK
                     KHYFPCSLPLFGGAAG"
BASE COUNT         3818 a         4530 c         4566 g         3911 t
ORIGIN      
        1 attagattaa aaatcaaaca atcaaagccc gcctgatata atcgatatat cctgttaaac
       61 gaaaaataaa tatgtttaaa aaattcaaac cggtattgtt gtcatttttt gcacttgtat
      121 ttgccttttg gctgggaacg ggcattgcct atgagattaa tccgcgttgg tttttgagcg
      181 atacggcaac tgaaatacct gaaaatccga atacttttgt ggcgaaactt gcccgcctgt
      241 tccgaaatgc cgacagggcg gttgtcatcg tgaaggaatc gatgaggacg gaggaaagcc
      301 ttgccggagc tgtggatgac ggtccgttgc agtcggagaa ggattatctc gcgctcgctg
      361 tccggctcag tcgtttgaaa gaaaaggcga aatggtttca cgtaacggag caggaacatg
      421 gggaagaggt ctggctggat tactatatcg gcgagggcgg tttggttgcg gtttcgcttt
      481 tgcaacgctc gccggaagcg tttgttaatg ccgaatatct gtatcggaac gatcgtccgt
      541 tttctgtaaa tgtgtacggc ggaacggctc acggggaaaa ttatgaaacg acaggagaat
      601 atcgggttgt ttggcaaccg gacggttcgg tatttgatgc gtcggggcgc gggaaaatcg
      661 gggaagatgt ttatgagcat tgcctcgggt gttatcagat ggcccaggta tatttggcga
      721 aataccggga tgtcgcgaat gatgagcaga aggtttggga cttccgcgaa gagagcaacc
      781 ggattgcatc ggactcgcgc gattctgtgt tttatcagaa tatgcgggaa ttgatgcccc
      841 gggggatgaa ggcgaacagt cttgtggtcg gctatgatgc ggacggtctg ccgcaaaaag
      901 tctattggag tttcgacaat ggaaaaaaac gccagagttt cgaatattat ttgaaaaacg
      961 gaaatctttt tattgcacaa tcttcgacgg tagcattgaa agcggatggc gtaacggcgg
     1021 atatgcagac ctatcatgcg caacagacgt ggtatttgga tggcgggcgg attatccgcg
     1081 aagagaaaca gggagacaga ctgcctgatt ttcctttgaa cttggaagat ttggaaaaag
     1141 aggtgagccg ttatgcagag gctgcggcga gacgttcggg cggcaggcgc ggcctttctc
     1201 actgaacgca aataccgtct gaaaccggtt ttcagacggc attggagagt caaacgcttt
     1261 ttgcaatgcc gtatatgccg aacccggttc gctacaaatt gaaaaccaca atccaaaaaa
     1321 cggaaataca cattatcaaa caaacaaata agttgacata ttgtgcgata ttgagggctg
     1381 taccgtattt tcccgttctt tatatgagcg gcaggcatcc tcataaaaga agggataccg
     1441 ataaggaaaa atccctcggt ataaaaaata tactgattaa tcaatacgaa tccaacaaac
     1501 agttcatcat tttcttttct gttggcgtat aaaaaccgct cggaaaaaaa taaaatatca
     1561 aaacggcaaa ataaaaatac tgatatatgc cttcactttc atagggcgac gctctatgtg
     1621 ccgtcctgtg tgttgaaacg tcggttttcc ttaccgcctg ccccgctttt ccctgattgc
     1681 tgccaactgc cgtccgaacc tgaaccgtca ggtttcagac ggcatttttt ctccctgttc
     1741 ccacaattgc cgtttttgtg cggcggtcag ttttttgacg gcgatttcct gtttgagcgc
     1801 agtgccttta tccatgccgc ctgcaacgat acgcatcgcc accggtttga atacgcgggt
     1861 atatttcgcg ccttttccgg ctgtgtggat ggcaagccgc tgttgcggat tcgggctgat
     1921 accgcaatag aacgcgctgt tttcacacaa tatcaggtaa acgctccagt ttgacgcgtt
     1981 catctgcctg catcctgggt tttttccccg ttccaaggcg caactttcag cagcagcagc
     2041 atacccggca gggcaagtat gaaacacagc cggaaaaacg gtacataacc catccattcg
     2101 atcagatagc ctgcaaagga attgatgacc gtgcgcggca cggcggacag gctggtaaac
     2161 agcgcaagct gcgttgccgt aaacgcggga ttggtctcgc gcgccatata cgatacaaac
     2221 gccgccgtcc ccaaccccac gccgaccgct tccgcgccga taactgccgc cagcatcagc
     2281 ctctcgcctg tgccgaccgt gtcgaaatgt ccgaaccctg ccagccatac aaaccccaaa
     2341 accgttattg cctgcaccgc gccgaatagc cacaaggctt tgtttacgcc gattttcagc
     2401 atccacacgc cgcccaagat acctgccgcc actgccggcc acagtcctgc atttttggca
     2461 atcaaaccga tgtcggtctt gctgaaaccc atatccagat aaaacggcgt tgccaacgcg
     2521 gttgccatac tgtcgccgag tttgtaaagg aagataaaca gcagcacgca caccgccgaa
     2581 gcgatgccct tgcgcgtaaa aaattcttta aacggctcta ccacggtctg cttcaacgtt
     2641 ttaggaacgg aaggcggcaa cacgggttcg tgcgcgagaa acagcgtcat cagaaggccg
     2701 ggcagcataa ataatgaagt gataacaaat acttctgacc acggcatcct gtctgccaac
     2761 accaaactca atgaaccggg aatcagggcg gcaacccggt aggcgttcac atgaaccgag
     2821 ttgcccaaac ccaattcttc gtccgacaaa atctcgcgcc tgaacgcatc caatacaatg
     2881 tcctgactgg cggaaaaaaa agcgacaagc accgacaggc cggcaatcaa cggcagatga
     2941 ttccgggggt ttaaaaaggc atatgccgcc aaagccgcca gtaaccctgc ctgcgtcagc
     3001 agcatccacc cgcgccgccg tcccaaaacg ggcagcctga ccgcgtccat cagcggcgac
     3061 cacaaaaatt tccaagtaaa cggcagaccg atcaacgcca tcagcccgat gctcttcaaa
     3121 tccacctgct cgctgcgcaa ccacgccgga atcaggttaa tcagaaagta cagcggcagc
     3181 cccgaggcaa accccgtaaa aatacagacg agcatattac gggaaaagat ttgcccgata
     3241 aaacctgatt tcgatgcagt catcgttcgt acagccaaaa aacaaaagcc tattgtagca
     3301 aatatcgccc gcctgctgaa aaatgccgcc acgcgccgcc ggtttgccgt tccaatgcaa
     3361 aatgccgtct gaaactgtgt tcagacggca ttgctactga aacccgacgg tttacaggct
     3421 gtaatacatt tcaaattcca gcgggtgcgg cgccatacgg atgcggcgga catcttcctc
     3481 tttaaaggcg atgtagctgt cgatccagtc tttgctgaac acgccgccgc gcagcaggaa
     3541 ttcgtggtcg gccttgaggg cggcaagtgc ttcttccaaa gaagcgcaga cggtcgggac
     3601 gagcgcgtct tcttccggcg gcaggtcgta caggttttta tcggcagggt cgcccggatg
     3661 gattttgttt tgaatgccgt ccaaaccggc catcagcagg gcggcaaatg ccaaatacgg
     3721 gttggcggtc gggtcgggga aacgcgcttc gatgcggcgc gccttgctgc tgttcacaga
     3781 cgggatacgg atggaagcgg aacggttttt ggcggaatag gccaatttgg tcggtgcttc
     3841 aaagtgcggc acgaggcgtt tgtaggagtt ggtggacgga ttggtaatcg cgttcagggc
     3901 tttggcgtgt ttgatgatgc cgccgatgta gtagagcgcg gtatcggaca aaccggcata
     3961 gccgtcgcct gcgaacaggt tttgaccgtc tttccaaatg gattggtgga cgtgcatacc
     4021 gctgccgttg tcgcccataa tcggtttggg cataaaggtg gcggttttgc cgaaattgtg
     4081 ggcaacgttt tggatgacgt atttcatatc ttgggtttgg tcggcgcgtt tgaccaaagt
     4141 ggcgaaacgg gtgccgattt ccatttggct gccggtaccg acttcgctgt ggtggacttc
     4201 gacttcgatg ccgagtcctt ccaaaatgtt caccatggcg gagcgcaaat cttgaccgca
     4261 gtcaatcggc gcgacgggcg cgtagccgcc tttgacggcg gggcggtggc cggtgttttg
     4321 accgtccata tgcaggccgc ttgcccacgc gccgctttcg gacgtgattt cgtaacgggt
     4381 tttgtgcatg tcggtttcaa attctacgcc gtcgaagacg aagaattcgg gttcggggcc
     4441 gaaataggcg gtgtcgccga taccggaaga tttcaaatag gcttcggcgc ggcgtgcgat
     4501 ggagcgcggg tcgcggtcgt aaccctgacc gtcggcaggg tcgatgacgt cgcaggtaat
     4561 gacgacggta acatcatcat aaaaaggatc gacgaaggct gtggacgcat cgggacgcag
     4621 ctgcatatcg gaagcctcaa tgcctttcca gccgccgatg gacgagccgt caaacgccgg
     4681 tccgttttca aaccactctt cggggtcttc caacacgatg cgcgcaggca cggtaaagtg
     4741 gtgctgcttg cctttggtat cggtgaagcg caaatcgaca aagcgggctt cgctttcttc
     4801 aatcaatttt acggcgtttt taatggacat cttcagctcc tgaaaaaggg aaatggcaaa
     4861 atatgcggaa cgcggcggct tgacgcaaag tcggcacgat tttaccacgc cgatgccgtc
     4921 tgaaaccggt tttgcgtcaa attccaagcc ggaaaatata gtttcaaacg gcaagattgt
     4981 caacaatatg cttcggtttg tctgttcaaa agcggaatgc ccgccctttt gtccgaatgc
     5041 cgacaaaaca gatgccgtct gaaggcggtt tcagacggca tcgttcccat cttcgcgccg
     5101 ccgttataaa atgaccgccc cgcctttatg gaatatgact atgcacgccc tcccccgcta
     5161 cgccgttttc ggcaaccccg ccgcccacag caaatcgccg cagattcatc agcaatttgc
     5221 ccttcaggaa ggcgttgaca tcgaatacgg acgcatttgc gccgacatcg gcggtttcgc
     5281 gcaggcggtt tcgacatttt ttgaaacagg cggttgcggg gcaaacgtta ccgtaccgtt
     5341 caaacaggaa gcgtttcatc tggcggacga acattccgac cgcgctttgg ctgccggtgc
     5401 ggtcaatacg ctggtgtggt tggaagacgg cagaatacgc ggcgacaata ccgacggtat
     5461 cggtttggcc aacgacatca cgcaggtcaa aaacattgcc atcgagggca aaaccatctt
     5521 gcttttgggc gcgggcggcg cggtgcgcgg cgtgattccg gttttgaaag aacaccgccc
     5581 tgcccgtatc gtcattgcca accgtacccg cgccaaagcc gaggaattgg cgcggctttt
     5641 cggcattgaa gccgtcccga tggcggatgt gaacggcggt ttcgatatca tcatcaacgg
     5701 cacgtccggc ggcttgagcg gtcagcttcc tgccgtcagc cctaaaattt tccgcgactg
     5761 ccgccttgcc tacgatatgg tttacggcga agcggcaaaa ccgtttttag attttgcacg
     5821 acaatcgggg gcgaaaaaaa ctgccgacgg actgggtatg ctagtcggtc aggcggcggc
     5881 ttcctacgcc ctctggcgcg gtttcaaacc ggacatccgc cccgtcatcg aacacatgaa
     5941 agccctgtaa ccatgttccg catcgtcaaa tggctgattg ccctgcccgt cggcatcttt
     6001 atcttcttca atgcctatgt gtacggcaac atcattacct accgcgccgt cgcgccccat
     6061 cggactgcct ttatgtcgat gcggatgaag cagtttgaac aagaaggtcg cgatgtcgca
     6121 ctggattacc gctgggtgcc ctacaaccgc atttccacca acctgaaaaa agccctgatt
     6181 gcttccgaag atgtccgttt tgccggacac ggcggcttcg attggggcgg cattcaaaac
     6241 gccatcaggc gcaaccggaa cagcggcgaa gtgaaggcgg gcggatcgac catcagccag
     6301 cagcttgcca aaaacctctt cctcaacgaa agccgcaact atctgcgcaa aggggaagag
     6361 gcggccatta cggcaatgat ggaagctgtt accgacaaaa acaggatttt cgaactgtat
     6421 ttaaactcaa tcgaatggca ctacggcgtt ttcggcgcgg aagctgcgtc ccggtatttt
     6481 tataaaaaac cggccgcaga cctgaccaaa cagcaggcgg cgaaactgac ggcactcgtc
     6541 cccgccccgc tctactacgc tgaccatccg aaaagcaaac ggctgcgcaa caaaaccaat
     6601 atcgtgctca gacgcatggg ttcggcagaa ttacccgaaa gcgatacgga ctgattgttt
     6661 cagatatgga aatgccgtct gaactgcggt tcggacggca tatgttttct ggttcttata
     6721 gtggattaaa tttaaaccgg tacggcgtta ccccgccttg ccgtactatg ttgtactgtc
     6781 tgcggcttcg tcgctttgtc ctgatttttg ttaatccact aacctgcccg acagccgcaa
     6841 agaccgtatc gtgcatggca ggtatttgcg gtgcgggact gctgccgaaa acggcaggca
     6901 aaaaataacc gcttataagc ggttttttgg tggccagagg cggaatcgaa ccgccgacac
     6961 acggattttc aatccgttgc tctaccaact gagctatctg gcctgtatct gtctcgttaa
     7021 gaggtgcgta ttaaaccaaa tttcgggacg acacgcaagc ggatttgtcg atatttttta
     7081 cccaaccgac taagtcgatg ttttaagttt aaattaattt cacagaaaaa tagtgcaaat
     7141 cggttttttg ccgttcgttc ggcgatgttc tgctttgcag acggcaaaac gcttcaatat
     7201 ttgaagttct cgcccaaata gacggcgcgc acctgttcgt tgccgaccaa atcgtcgggc
     7261 ttgccggaag ccagcaccgc accgtctgaa atgatgtagg cgcggtcgca gatgctgagg
     7321 gtctcgcgga cgttgtggtc ggtaatcaat acgccgatgc cgcgcgattt gaggaaaccg
     7381 atgattttct ggatgtcgat gacggcgatg ggatcgacgc cggcaaaagg ttcgtccaaa
     7441 aggatgaaac gtggtttcat ggcgaggaca cgggcgattt cgacgcgccg ccgttcgccg
     7501 ccggacagcg acggcgcagg attgcggcgc aagtgtccga tattgaggtc ggcgagcagt
     7561 ttttcgattt ctctgtcgat ttgattttta tctttggttc tgatttccaa gatggcgcgg
     7621 atgttttgtt cgacggtcat tttgcggaat atcgaggctt cctgcggcag gtagccgaca
     7681 cccaggcggg cgcgttcgtg tatgggcagg tggcgcaatt cccgcccgtc cagcatcacg
     7741 ctgcctgcgt cggcagcgat gagcccgact atcatgtaga agccggtggt tttgcctgcg
     7801 ccgttgggcc cgagcagtcc gatgacttcg ccgctttcga tctcaaggga gaaacttttg
     7861 acgacttggc gttttttgaa acttttctgc aggttttgga cgacgaggcg gctgttgttt
     7921 gcgctcataa atctcttctc ggcatttcgg tcggcaaatg ccgtctgaac tgcgtttccg
     7981 tttcagacgg catcggggtt attcggtttt ttgtgtgctt gaaggctgga tgacgacgct
     8041 gaccctgccg gttttggaag cggatttcgc acccgatttc gtgctgccgt tgatggtata
     8101 gacttcggtt ttggtgttgt aggtaatgac cgcaccttcg gcaacgtcgc cgccgcgctg
     8161 cactttggca ttgccggtca gaacgacggt gcttcctgcg gaggaatagg taacgttgtt
     8221 tgcctgaccg cgcaccgtcc ctttgccccc gtccaacgtt tggctgaagc ggacgggcga
     8281 accttccgcc ctcacggatt cgccgccttt gccgccgcgt gtgacgttga cgcgcgaggc
     8341 ggaaatgttg agcgtaccct gtctgatgat gacattgccg ctaaatgtgg tactttggtt
     8401 ggcttgatcg agcgaacctt ggtcggcttc gatttggatg ggccgtctgc tgtcgctttg
     8461 aagggcaaaa gcgggagaag ttgcaaaaat tacaattaaa acaaatagct tacatatctt
     8521 ttgtatcata aatcgcggct ttcactttag atgagaagtt caacatgcct gttttgtggt
     8581 tgtaggtcat accgcccgcc tgaccgtgcg acgcgccata ttggaaactg acaggcgtat
     8641 cggtttgggc atattgagat tcggtatcga cgtgcagttt ttcggtttcg actttacccg
     8701 cctgccgcct gccgtcggcg gttttggtca gcacaacgtt gtttttaaaa agaacctgtt
     8761 tgttttcggt atggtaaacg gcttcatcgc tgccgacttc gtacaacagc ctgccttctt
     8821 ggaagaacac gagatgcggc gaatcaaaat ggatgtcgct gttttcggga aactgtttcg
     8881 cacctttcgc gctcaaatgt tctttcaagt atccctgttc gtcaaaccgc cttccgtcca
     8941 agccgtccat tgtgtattga ggttcgtcgg gattgagcct gacttcctcg atttcgactt
     9001 cgctgatacg gcccaaccat gccgacaggc tgcccaaggc aaccgccaat atcaatggga
     9061 acgcaattcc gtaccgccat cttactttca tttgatgtac ccgttcaaag ccgcgcccaa
     9121 cgtacctttc gcgcggataa tcaggtcaca cacttcgcgc accgcgcccg caccgccggg
     9181 gcgtttggca atataggcgg cgtgttgcag cgtaaaccaa tgcgcgtcgg ggacggcaac
     9241 cggcaatccg cagcgcacca tcaccggcaa atcgaccacg tcgtcgccga caaaggcgca
     9301 ctcggcttct tccaagcctg cctgcgcgcg cagctcttca tacgcggcgc gtttgtccga
     9361 aatgcctttg aagtagtaat ttatgcccaa ctgtttgacg cggatgccga cggagggcgc
     9421 gtcccgacct gtgataatcg cagtctgcac gccgcttgcc tgaagcattt tcaggccgtg
     9481 tccgtccagt gtgtgaaacg atttgatttc ttcgccgtcg tcgcggataa agatgcgccc
     9541 gtcggtcaaa acgccgtcca catccaatat cagcagtttg attttggcgg cgcgtgcctg
     9601 taattcggga gaaattgcct gcattgggat ttccttgttt tcagacggca tatcttacca
     9661 caaatgccgt ctgaaacggc tgcaacgcct tatacaatcc gcgccgccaa aaggtcgtgc
     9721 atattcagcg cacctgtcag cacgccgtct gcatcggtaa ccagaagccc gttcacatgg
     9781 tttgcctgca tgactttcag ggcttcggtg gcgaggcgtt cggcggagat ggttttaggg
     9841 tgtgtatgca tgacttcgtc tatcgaaaga ccggtaaaat tgtcgcattc ttgaaacagg
     9901 cggcgcaaat cgccgtcagt aaacacgcct ttcagacagc cttgcccgtc cgttaccgcc
     9961 aacatgccca aacctttctc gctcatgctg acgatggctc ctttcaaggg cgtgccgagt
    10021 cggacggcgg gcaggccgcc gcctttgtgc ataatgtcgg caacgcgcaa aagcaggcgt
    10081 ttgccgaggc tgccggcagg gtggatcaag gcgaagtcgt cgggcgtgaa cgcgcgggcg
    10141 cgcagcagga cgaccgccaa cgcgtcgccc aaagccataa cggcggtggt gctggtggtc
    10201 ggggcaagcc ccaacgggca ggcttcttgc gaaaccgatg cggtgatgtg gatatcggca
    10261 tggcgcgcca tggttgaatc ggggcgggcg gtgatgcaga cgagggtaat gtctttgcgt
    10321 ttgagcgcgg ggatgatggc ggtgatttcg tcgctttcgc cggaattgga aatggcggcg
    10381 acgacgtcgt tgtccacaat catgcccaaa tcgccgtgtg ccgcttccgc agggtggacg
    10441 aaaaacgcgg gcgtgccggt cgaggccatg gttgccgcca ttttgcgccc gatatgtccg
    10501 gacttgccca tacccgtgat gacgaccctg cccttgcagt gcaacaacgc gtctgccgca
    10561 aggacgaagt tttcgtccaa ttccgccgca atttcgcgca agccttccgc ttcggtgtgc
    10621 aacacttcgc gcgcccagtc gagatatttt tcgttttctg ccattgccat ccgcctttgc
    10681 tgtaacaaaa ctacccaaaa tttccgaatc ggggtagcat aagccttcaa accccgccca
    10741 tccatccaaa ggaatcaaaa atgactattt tatcggacgt taaagcatta ggacaacaaa
    10801 tctggctgga caacctttcc cgctcgctcg tgcaaagcgg cgaattggcg caaatgctca
    10861 aacaaggcgt gtgcggcgta acttccaatc ccgccatttt ccaaaaagcc ttcgccggcg
    10921 acgcgcttta tgccgacgag gtcgccgccc tcaagcggca aaacctcagc cccaaacaac
    10981 gctacgaaac catggcggtt gccgacgtac gggcagcctg cgacgtttgc cttgccgaac
    11041 acgaatccac cggcggcaaa accggtttcg tcagtctcga agtttctccc gaacttgcca
    11101 aagacgctca aggcacggta gaagaagccc gccgcctcca tgccgccatc gcgcgtaaaa
    11161 acgccatgat taaagtgcct gccaccgacg caggcatcga tgcgctcgaa accctcgttt
    11221 cagacggcat cagcgtgaac ctgaccctgc tgttctcacg cgcccaaacc ctcaaagcct
    11281 acgccgccta cgcgcgcggc attgccaaac gcttggcagc cggacaaagc gttgcccata
    11341 tccaagttgt cgccagcttc ttcatctcgc gcgtggacag tgcgctggat gcaacgctgc
    11401 ccgaccggct caaaggcaaa accgccatcg cccttgccaa agccgcctat caagattggg
    11461 aacaatattt caccgccccc gaatttgccg cactggaagc ccaaggcgca aaccgcgtgc
    11521 agcttttatg ggcatctacc ggcgtgaaaa acccagccta tcccgacacg ctctacgttg
    11581 acagcctgat cggcgtgcac accgtcaaca ccgtccccga tgccacgctc aaagccttta
    11641 tcgaccacgg cacggcgaaa gcgacgctga ccgaaagcgc ggacgaagca cgggcgcggc
    11701 tcgccgaaat tgccgcgctc ggcatcgatg tcgaaacctt ggcggcgcgt ttgcaggaag
    11761 acggtttgaa acagtttgaa gaagcctttg aaaaactgct cgcgcctttg gtttaggcat
    11821 ttccccgatg ccgtctgaaa ggctttcaga cggcatcgcc cttttgaccg gacacagtgc
    11881 ggcatatcaa ggatccgccc taaaaatatt aatgggcaag aagttaatgc acgacattac
    11941 ccgccaacac aaaaggaaat agaatcatgg caaaagcact cgaaatcatt tcacccgatg
    12001 aaatttattc agatctgatt tttaaggatc cggtacctcc ccatactata tatacagaat
    12061 tgatgaaact ggttggtaga aatgcaggca atgagagaat tgcagataga gccttcgatt
    12121 ttttctcgcc tgctcttatg gatgattcag ctaccgaaga gcaatataat gccctatatg
    12181 atttaacctt gcttgaagag cccgggatgg aactggataa agatgagata acggctttga
    12241 ttaatagtct taaataaagg ggctgtaccg gatatgtaca caggacgctt cgcccccagc
    12301 ccgaccgggt tgctccacat cggctctctt ctgaccgccg tcgcttccta tgccgatgca
    12361 cgttcaaacg gcggcaaatg gctggtccgt atggaagacc tcgatccgcc gcgcgaaatg
    12421 ccgggggcgg caagccatat cctgcacacg ctcgaggcat tcggattcga gtgggacggc
    12481 gaagtcacct atcagagccg ccgttacgcc ctgtatgaag aaaccctata ccgtctgaaa
    12541 accgccggac tggtctatcc ctgccattgc aaccgcaaag actggcaggc cggggcaagg
    12601 cggggcacag acgggttcgt ctataacgga cgctgccgcc accccggcca acgccctgca
    12661 ctgcaaggca aacagccgtc gtggcgcatc cgcgtccccg accgcgacat cggcttttca
    12721 gacggcatcg tcggcagtta cacccaaaac ctcgcccgcg acatcggcga tttcgtcctg
    12781 ttccgtgcag acggttactg ggcataccag ctcgccgtcg ttgccgacga tgccgaacag
    12841 ggcgttaccc acatcgtgcg cggacaagac ctgctcgttt ccacaccgcg ccaaatctat
    12901 ttgcagcagt gtttgggcgt tccgacaccg caatatgccc acctgccgct gctgaccaac
    12961 gcgcaagggc agaaatggtc gaagcagacg ctcgcccccg cattggattc aaaccgccgc
    13021 gaacaacttc tccgccaagt gttccgctac ctcaagctgc ccgaagcacc ggaaaccgac
    13081 cgccctgccg aattgctcga ctgggcggtg gcacactggg atatggacaa agtgccgaaa
    13141 cacgccatta ccgcccccta aacaccaccc gacaaacgca caaatgccgt ctgaaacctt
    13201 cagacggcat ttcacacata cagccccgcc ctattcgcgc gtatttgccc gttcgacttc
    13261 tttccacgct tcgaaaatca ggccgccgtc gttgtctttc aagagcgttg catcggaaag
    13321 catacgccgc caagtccgcg cgcctttcaa accgtgcatc agcccgaggc tgtggcggac
    13381 gatgtggcgc aggattgtgc cgcgtccggc ttggatttgg acttggctgt atgtgtagag
    13441 gcgctgcacc aaatcggcgt attcgatcgg ggcgcggttg tcaccgtaaa acagcctgtc
    13501 ccattcgcgc atcaccatcg ggttgtggta tgcctcgcgc ccgaccatca cgccgtcaac
    13561 gtgttgcaag tgtccggcga ttgcttcgtt ggtggtgatg ccgccgttga tgatgatttc
    13621 cagctcggga aactcctgct tgaggcggta aacgtaatcg tatttcaagg gcggaacatc
    13681 gcggttttct ttgggcgaaa gaccgtccag ccaagcgttg cgggcgtgga cgatgaaggt
    13741 tttgcaggcg gttttgtcgc gcagcgtgcc gacgaaatcg gcaacggttt ggtattcggt
    13801 ctgcctgtcc acaccgatgc ggtgtttgac ggtaacgggg atgccgaccg cgtcgtgcat
    13861 ggcgttgagg cagtcggcaa ccagcccgac ttcgttcatc agacacgcgc cgaacgcgcc
    13921 tttctgcacg cgcggactgg gacagccgca gttgaggttg acctcgttgt agccgtattc
    13981 ttcggcggct ttggcggctt tcgccaaatc ggacggatcg ctgccgccca gttgcagggc
    14041 gacgggctgc tcgccttcgt taaacatcaa aaaacggtct ttgtcgccgt aaacaatcgc
    14101 gccggcgttg accatttcgc tgtaaagcca agcatttcgg gtaatctggc gggcaaggta
    14161 acggtagtgc ctgtccgtcc agtcgagcat cggggcgacg cataaacgcc ttttcccttt
    14221 atgttcaata agttccttgt ttatcataag gttttcacat tcttttaact caaacattct
    14281 ttattatttg cttggatttt tgcccatttc actatatttg acgtaccata cttgcaccat
    14341 aatttttaat tcttggaatg gtgcaaatgg caacaataac caagcggcgc aatccttccg
    14401 gcgaaacagt atatcgggtt caggtacggg tcggcaagaa aggctatcct gctttcaatg
    14461 agagcaggac gttcagtaaa aaggctttgg cggtcgaatg ggggaagaaa cgggaggcgg
    14521 aaatcgaagc cggcccagaa ctgcttttca agcgcggcaa ggtcaagatg atgacgctgt
    14581 ccgaagctat gcggaagtat cttaacgaaa cgcttggagc gggtcggtca aagaaaatgg
    14641 gcttgcgttt tctgatggag ttcccgattg gcggcatcgg catcgataag ctgaaacggt
    14701 ctgatttcgc ggaacacgtt atgcagaggc ggcgcggaat ccccgaactg gacattgcgc
    14761 caatcgcggc ttctacggca ttgcaggagc tgcaatatat ccgttccgtg ctaaaacacg
    14821 ccttttatgt gtgggggctt gaaataggct ggcaggaatt ggatttcgcg gcaaacgggc
    14881 tgaaacgctc gaatatggtt gcgaaatctg caatcaggga cagattgccg accacggaag
    14941 aactgcaaac cctgacaact tatttcctgc ggcaatggca aagcaggaaa tcttccatac
    15001 cgatgcacct gattatgtgg ctggcgattt atacgtcaag gcggcaggat gagatttgcc
    15061 gcctgctgtt tgacgactgg cacaaaaatg attgtacccg gccggttcgt gatttaaaaa
    15121 atccgaacgg cagcacaggg aataataagg agtttgatat tctgcctatg gctttgccgg
    15181 tcattgacga gctgccggag gaatcggtca ggaagcgtat gcttgccaac aaaggcatcg
    15241 ccgacagcct tgtaccgtgc aacggaaaat cggtttccgc cgcttggacg agggcgtgca
    15301 aggttcttag aattaaagac ttgcgcttcc acgatttacg gcacgaggct gctacccgta
    15361 tggctgaaga cggcttcacg attccgcaaa tgcaacgggt aacgctgcat gacggttgga
    15421 acagcctgca gaggtatgtg agcgtacgca aacgctcgac gcggctggat tttaaagagg
    15481 caatgatgca ggcgcaaagc gatataaaat ccgggaagtg atgttaaatt aaaggggatg
    15541 tgccgcatcc cctgattcct ccgtcttgca tcaagaccgg aatttccgat attccgattt
    15601 tgccttttct tccttgtctg cgattgcctt tgcaagctct cggacgctga cgaaatattc
    15661 cgattttcgg ctgtcggaca atcggaaaat cggaaacgga agctcgcacc gtacccccga
    15721 tgctttaaac gcgttaaagc cgatatgggc aaaaaagtct ttgtgtactt ccctcggcgg
    15781 gatatacggg cggccgccgt atgcgattaa aagcccttgt tctttactga tgttcatata
    15841 tacctctttc tgttaattct gctttgtccg ggtttccgct tttttacggc ttggcggatg
    15901 ttcagcctgc gtcggtgcat aagtcctcct gttcgggcgg ttgttcagcc ggccgcccct
    15961 ccgaaaagtg gcaggctgca cgggaagtag tgtttttttc cgtatttctc tgcctgtatc
    16021 aggttgcaca ggcggtcgca aaagccgtca aattcggcat gcggccagcc ttctatctcg
    16081 ttgaccattt tgctgaacct caattcccgg gcaatccgct ttgccgctgc gtcacggcat
    16141 tcgtgttcca agaggacggg gtcttccttt tccttcggcg gtgcaggttc ttgcacggtt
    16201 tgccacaagt ccggttcgat ttgccaagtg tcggcggcaa gcagggtgtc tgttttgcag
    16261 tcgtacagtt cgcggatgca gccgccgtcg gttttgtctt tgtcgataac cagtaaaagc
    16321 acttcgatcg gcgtgtcttc aaacgcgttt tgtatgcggt tgagttctgc cagccggttg
    16381 ccgatgattt cgcggaatcg ctgctcgctt ttgcggtagg cgatgccggg gaacaggatg
    16441 aagaatccga agcggcgggc gttttccaat ccttttaaaa cgaagatttc atcggctacg
    16501 cccgatttct tccacgggta ttcctgtgcg atacggcttt tttcgtcttc gctcaaatcc
    16561 tttagcttga tggaaaacgg cggattcatg acggtgcagt cttgcggttc gccgtcggta
    16621 taaaggaaaa ggcttgtgtt gtatactttg gcggcagggt agttttgcaa caaggcttta
    16681 cacgcttccg cctgtatctc tacggcgcgg aaatcggacg gttcgatgta ctgctccagc
    16741 tgcccgcttc ctgccgcgcc gtcaaatacg cccgggtgtt cgccgcagta tcggcggact
    16801 ttggcggcaa ccagccgcct caagc
//
LOCUS       DAXDSD010000046        16642 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXDSD010000046 DAXDSD010000000
VERSION     DAXDSD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16642
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.046"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(201..686)
                     /gene="purE"
                     /locus_tag="ACFB2Q_001146"
     CDS             complement(201..686)
                     /gene="purE"
                     /locus_tag="ACFB2Q_001146"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC0918178.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(734..1447)
                     /locus_tag="ACFB2Q_001147"
     CDS             complement(734..1447)
                     /locus_tag="ACFB2Q_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC0918179.1"
                     /translation="MKTKLPLFIIWLSVSASCASVSPVPEGSRTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVEMLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1447..2115)
                     /locus_tag="ACFB2Q_001148"
     CDS             complement(1447..2115)
                     /locus_tag="ACFB2Q_001148"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC0918180.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2126..2311)
                     /locus_tag="ACFB2Q_001149"
     CDS             complement(2126..2311)
                     /locus_tag="ACFB2Q_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC0918181.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2455..4431
                     /gene="mutL"
                     /locus_tag="ACFB2Q_001150"
     CDS             2455..4431
                     /gene="mutL"
                     /locus_tag="ACFB2Q_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC0918182.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4526..6649
                     /gene="dnaX"
                     /locus_tag="ACFB2Q_001151"
     CDS             4526..6649
                     /gene="dnaX"
                     /locus_tag="ACFB2Q_001151"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC0918183.1"
                     /translation="MTYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTASEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5715..5754
                     /gene="dnaX"
                     /locus_tag="ACFB2Q_001151"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6730..7065
                     /locus_tag="ACFB2Q_001152"
     CDS             6730..7065
                     /locus_tag="ACFB2Q_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC0918184.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACFB2Q_001153"
     CDS             complement(7718..8764)
                     /gene="recA"
                     /locus_tag="ACFB2Q_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC0918185.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8964..9728
                     /gene="aroD"
                     /locus_tag="ACFB2Q_001154"
     CDS             8964..9728
                     /gene="aroD"
                     /locus_tag="ACFB2Q_001154"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC0918186.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9748..11763
                     /gene="rep"
                     /locus_tag="ACFB2Q_001155"
     CDS             9748..11763
                     /gene="rep"
                     /locus_tag="ACFB2Q_001155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC0918187.1"
                     /translation="MMKLNAQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACFB2Q_001156"
     CDS             complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="ACFB2Q_001156"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC0918188.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATVKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     PDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
     gene            12990..13199
                     /locus_tag="ACFB2Q_001157"
     CDS             12990..13199
                     /locus_tag="ACFB2Q_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918189.1"
                     /translation="MKLDKNNGFYQAERNSNGNYDIVRKNYLKNITKIQSNILIYLDN
                     TSTGFKIQQRRKQIRAQISARQWRR"
     gene            13791..14570
                     /locus_tag="ACFB2Q_001158"
     CDS             13791..14570
                     /locus_tag="ACFB2Q_001158"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC0918190.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            14779..15849
                     /locus_tag="ACFB2Q_001159"
     CDS             14779..15849
                     /locus_tag="ACFB2Q_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC0918191.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAEHHRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKDNTDINDITTAGVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRNPATTARPLLNTSKPKPQPHIRPDELPDF
                     YRTLQTAHDLHPYARPLLLLAALTVPRPSALLSARWQDIDLTARLWHIPAADMKTKHP
                     FTVPLSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYD
                     GSTPANPNTPCTDSATSSPTSA"
     gene            15810..16058
                     /locus_tag="ACFB2Q_001160"
     CDS             15810..16058
                     /locus_tag="ACFB2Q_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918192.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            16078..16341
                     /locus_tag="ACFB2Q_001161"
     CDS             16078..16341
                     /locus_tag="ACFB2Q_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918193.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            16378..>16642
                     /locus_tag="ACFB2Q_001162"
     CDS             16378..>16642
                     /locus_tag="ACFB2Q_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918194.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKK"
BASE COUNT         4249 a         4933 c         4102 g         3358 t
ORIGIN      
        1 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat atttcaaaaa
       61 ttgaaacccg cacagattgt taaaccccaa aacaggggcg ggcgaaattc ttccggcaat
      121 gggttcatgc aaacaaaatg ccatctgaac cccgaaaaca gggcttcaga cggcatttgt
      181 gtattcgcgg acggcgggtt ttaaatctgt tccaattcca tgtttaaaac ggtttgttcc
      241 tgtttggctc ggaagtctgc cagtttttgc gccagttcgg ggttttcgtt ggcgagcatg
      301 gagatggcga acaatgcggc atttgccgcg cctgcctcgc cgatggcgaa tgtggcgacg
      361 ggtacgcctt tgggcatttg tacaatcgat aaaagcgaat cttcgccgcg caggtatttg
      421 cttgggacgg gtacgcccaa aacggggacg gtggtcttgg cggcaaccat gccgggtaaa
      481 tgcgccgcgc cgcccgcacc cgcgatgatg gctttgatgc cgcgcgcccg tgcggtttcg
      541 gcgtattcaa acatcaaatc cggagtgcgg tgtgcggaaa cgacgcgcgc ctcatattct
      601 acgccgaact cttcaagaaa ctgcgctgcc tgccgcataa cgggccaatc gctgttgctg
      661 cccatgatga tgccgatttg tatcataaat ccttcttggt gcggatgggg taaaaagcgg
      721 aaaaatggaa aaactatcgt ttgcgtacgg ctgcggcggc gcgttttgcc gccgggctgc
      781 cgggataggt ctgtatcagg ctgcgccaag tcgcccttgc aatgtctttt tgctgaagcc
      841 tgtattggca ttcgccgatt ttgaatatga cttcgggcgc ggttgggctg tctttgaaac
      901 ggttggcgta acgccctccg atttcgatga cagattcaca gttccccata cgcgccctgc
      961 tttgcagcaa caggtacata ctgcgttgcg cgatgctgcc gccgtctccg ccgtccgccc
     1021 ccttcaacaa ggcggctgcg gcagaaaacc tgccgttttg atagtgtttg agtgcctgat
     1081 tgtagaggtt ttgtgcggtt tcgacggtat gtgcggatgc gctgccgcct tcggtattga
     1141 ggtaatgctc tttcaatttg cggtcgtcga gtttttggac gtatgtcctg ccggaagggt
     1201 gtgtttttgc gtgttccaat gctttgactt tcccgtttaa catttccact tcgttcgaca
     1261 gccggacgat tttgccttcc agatagtcca aacggtcttg cagagtggga acgggatagg
     1321 gaatgccgtc tgaagcattt tcctgtgtcg gcatttcggt tcggctgccc tccggaacgg
     1381 gtgaaacgga agcacaggag gcagacacag acagccaaat gataaaaagc ggtaatttgg
     1441 tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg tgatggggac
     1501 gatgcgcgta tcgttcggca ggttttgatt gaaatctttg agaattttga cactgggcgg
     1561 cgcatcaaaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt cgatggcgat
     1621 gatgccgcct tgccggacga gtttgaggca acgctcgaaa tattgcggcg tgggcggttt
     1681 gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa tcaaatcatc
     1741 caatgtcagc aatgcgggtt gcaggtgcag gctgatttta tgcgccacac cggcctcgtt
     1801 ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg taatccgccc
     1861 gtgttcgggc agcgccaatg caagcgcggt gctgctgtat ccggtaaata cgccgatttc
     1921 cagatatttt tccgcgcgaa tcagctttgc cagccaaacc aaaaccgccg cctgttcgcg
     1981 cgcaatcgcc attttgccca tacggtgatg cccggccttc tcacgcagcc gcgtcaaaac
     2041 gggatgttcg ggttcgccga tggcattcaa atagttttgc aggtccggtg cgacattgga
     2101 cagatgggtc gtcatttcgg cgggttcagt cttggtaata ggtataaggt tttttcgcca
     2161 cttttgccga ttcaaagttt ttttgctctt cgggattgag ttcgacatcc cacaaaagcc
     2221 ctcgattttt caaacgttct tgcgccaatt caggtttttc ttcgatcagg cggttgagga
     2281 attgtgtggc ttcggattgg tagtggtaca tctttgcgct ccaattttac gggatacggc
     2341 gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat acgggcagaa
     2401 agataagggg ttttattata gaataaggcg ttttttgcaa cggaagaccg ccttatgcca
     2461 cgcattgccg ccctgcccga ccacctcgtc aaccaaattg ccgccggcga agtggtcgaa
     2521 cgccctgcca acgccctgaa agaaatcgtt gaaaacagca tagacgcggg cgcaacggcg
     2581 gttgatgtgg aactggaagg cggcggcatc cgcctcatcc gcgtcggcga caacggcggc
     2641 ggcatccacc ccgacgacat cgaactcgcg ctccaccgcc acgccaccag caaaatcaaa
     2701 accctgaacg atttggaaca cgttgccagc atgggctttc gcggcgaagg cttggccagt
     2761 atcgcctccg tcagccgcct gaccctgacc agccgccaag aagacagttc gcatgcgacc
     2821 caagtcaaag ccgaagacgg caaactcagc agccccaccg ccgccgccca ccccgtcggc
     2881 accaccatcg aagccgccga actcttcttc aacacccccg cgcggcgcaa gttcctcaaa
     2941 tccgaaaaca ccgaatacgc tcactgcgcc accatgctcg aacgcctcgc gctggcgcat
     3001 ccgcacattg ccttctcact caaacgcgac ggcaaacaag tgttcaaact tcctgcccaa
     3061 agcctgcatg aacgtatcgc cgccattgtc ggcgatgatt ttcagacggc atctttggaa
     3121 attgacagcg gcaatagcgc gctgcggctc tatggcgcga ttgccaagcc gactttcgcc
     3181 aaaggcaaaa ccgataaaca atattgcttc gtcaaccatc gttttgtccg cgacaaagtc
     3241 atgctccatg ccgtcaagca ggcataccgc gacgtattgc acaacgccct cacccctgct
     3301 ttcgtccttt tcctcgagct gccgcccaaa gccgtggatg tcaacgtcca cccgaccaaa
     3361 accgaaatcc gcttccgcga cagccggcaa gtacaccaac tcgtgttcca cacgctcaac
     3421 aaagcccttg ccgacacacg cgccaacctg accgaaagcg tcagcaacgc aggcgaagtg
     3481 ttgcatgaca ttaccggcgt tacgcctgcc ccaatgccgt ctgaaaacga cagcgaaaat
     3541 ctgtttgata gcgcatccaa ccacccgaca ggcaacaaac ccgatacacg caatgccttt
     3601 ggttcatcag gtaaaaccgc gcccatgcct taccaggccg cgcgtgcgcc gcaacaacac
     3661 agcctgtccc tgcgcgaaag ccgcgcggca atggacacct atgccgaact ctacaaaaaa
     3721 accgacgaca tcgaccttga gttgagccaa ttcgaacagg cacgtttcgg caatatgccg
     3781 tctgaaacgc ctgctcacaa aacagatacg ccgctttcag acggcatccc gtcccaatcc
     3841 gaactgccgc cgctcggttt cgccattgcc caactattag gcatctacat tcttgcccaa
     3901 gccgaagaca gcctgttgct catcgatatg cacgccgctg ccgaacgcgt caactacgag
     3961 aaaatgaaac gccaacgtca ggaaaacggc aacctgcaaa gccagcacct gcttattccc
     4021 gtaacctttg ccgcgtccca cgaagaatgc gccgccctcg ccgatcatgc cgaaacgctg
     4081 gcaggcttcg gcctggaact gtccgacatg ggcggcaaca ccctcgccgt ccgcgccgcg
     4141 cccgtaatgt tgggcaaatc cgatgtcgtc tccctcgcac gcgacgtatt gggcgaactc
     4201 gcccaagtcg gcagcagcca aaccatcgca tcacacgaaa accgcatcct cgccacaatg
     4261 tcctgccacg gctcaatccg cgccggccgc aggctcaccc tgcccgaaat gaacgcgctg
     4321 ctgcgcgata tggaaaatac gccgcgcagc aaccagtgca accacggcag gccgacgtgg
     4381 gtcaaactga ctttgaaaga attggacaca ctgttcttgc gcggacagta agccgaaaat
     4441 gctagaatac gccgcccgaa gccgccgttc agacggcatt ccgacgcacc gacagaaaca
     4501 tcacgaccga aaccaagaga aaaacatgac ctatcaagtt ctcgcccgaa aatggcggcc
     4561 caaaaccttt tccgacttag tcggtcagga acacgtcgtc aaagccctgc aaaatgcctt
     4621 ggacgaaggc aggctgcacc acgcctacct gctgaccggg acgcgcggcg tgggcaaaac
     4681 caccatcgcc cgcatccttg ccaaaagcct caactgcgaa aatgcgcaac acggcgaacc
     4741 ttgcggcgtg tgccaaagct gcacccaaat cgatgccgga cgctatgtcg acctgctcga
     4801 aatcgacgcc gcctccaaca caggcatcga caacatccgc gaagtcttgg aaaacgccca
     4861 atacgcgccg accgccggca aatacaaagt ctatatcatc gacgaagtgc atatgctttc
     4921 caaaagcgcg ttcaacgcca tgctcaaaac gctggaagag ccgcccgaac acgtcaaatt
     4981 catcctcgcc accaccgatc cgcacaaagt ccccgttacc gtattaagcc gctgcctgca
     5041 attcgtctta cgcaatatga ccgcgcaaca ggttgccgac caccttgccc acgtcctcga
     5101 cagcgaaaaa atcgcctacg acccccccgc cctgcaactt ttgggacgtg ccgccgccgg
     5161 atcgatgcgc gatgccttaa gcctgctcga ccaagccatc gccctaggtt cgggcaaagt
     5221 tgccgaaaac gatgtccgcc aaatgatcgg cgcggttgac aaacaatacc tgtacgaact
     5281 gctgacaggc atcgtcaacc aagacggcga agccctgctg gcaaaagcgc aggaaatggc
     5341 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactg gccatactgc tgcaacaact
     5401 cgccctgata caggcagtcc cctccgcctt ggcgcacgac gaccccgatt ccgatatttt
     5461 gcaccgcctc gcccaaacca taagcggcga acaaatccag ctttactacc aaatcgccgt
     5521 ccacggcaaa cgcgacctgg gcctcgcccc cgacgaatac gccggcttta tgatgaccct
     5581 gctgcgtatg ctggcgttcg cccccttggc ggcggcttcg tgtgatgcaa acgccgtgat
     5641 tgaaaatacc gaacttcaat ccccgtcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
     5701 aaagccacaa ccgcgccctg aagcggatgc cgcccaaacg cccgttcaga cggcatccgc
     5761 agcagcaatg ccgtctgaag gcaaaactgc cggccccgtt tcccatcaag aaaacaacga
     5821 tgttccgcct tgggaagacg cgccggacaa aaccgaaacc gcagccggca cggcgcgaac
     5881 gtcggcaaaa agcattcaga cggcatccga agccgaaacg ccgcccgaaa atcaagtttc
     5941 caagaacaag gcagccgaca acgaaaccga ggcttccttg tccgaagtgc cgtctgaaaa
     6001 ccccattcag gcaacaccga atgatgaagc cgttgaaacg gaaacatttg cacacgaagc
     6061 ccctgcagaa cctttctacg gttttccgga taatgactgc cccccggaag acggcgtgga
     6121 aatcccgccg cccgattggg caaatgtcct ccctgccgat accgcaggcg gagggacgga
     6181 cgaagaagca gaagcgggcg gcatcggcgg aaacaacacg ccgtccgccc cgccgcccga
     6241 attttccacc gaaaactggg cagccatcgt ccggcacttc gcccgcaaac tcggcgcggc
     6301 gcaaatgccg gcgcaacact ccgcgtggac ggaataccgt tccgacaccg gactgatggt
     6361 tttggcaatg accgccgaag cccgcgccac cgccgacaaa aagcggctcg acaaaatccg
     6421 cgacaccctc gcccaagcct acgggctgca actcaccctg caaaccgaag actggcgcga
     6481 cgaagccggc cgggaaaccc ccgcgatgca ggacaagcgt gtccaagccg aagacaggca
     6541 aaaagcacaa gcattgctcg aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg
     6601 cgcgcaatgg cagcccgaat cactggaatt ggcggcaaac cggccataaa cagatataat
     6661 gccgcccgaa cccttcggac ggcattgccg cttccctcat tcattcaaac acaaacagga
     6721 gtattcagta tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg
     6781 caggaaaaca tgaaaaaggc acaagccaaa cttgccgaaa ccgaaatcga aggcgaagca
     6841 ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc
     6901 agccccgatt tgattcaaga agccgccgat gacaaagaaa tgctcgaaga cctcatcctc
     6961 gccgccctca aatccgcccg gggcaaagcc gaagaaaccg caaacaaaac aatgggcgca
     7021 ttcacccaag atctgccccc cggcgtgggc gacttcttcc gctgaccccg accgtcattc
     7081 ccgcgaacgc gggaatccgg taaccgaaaa accacaggaa tctatcggaa aaaaacagaa
     7141 acccccgccg ccgtcattcc cgcgcaggcg ggaatctaga acgtagaatc tgaaaaaccg
     7201 ttttactcga taaatttccg tgccgacagg tctggattcc cgccttcgcg ggaatgacgg
     7261 catatttttt gcatttgata taaagggtcg tttgaatttt gttcagcaag tgcaaagtat
     7321 tgcacataaa agggcgcagg cttcatccgt aaagtcggcg caggatagag gcaaagcggg
     7381 cgtaggtcgg gctgtagcaa ctgtattttt caccccgtcg ggtaaaaata tagtggatta
     7441 acaaaaaccg gtacggcgtt gcctcgcctt agatcaaaga gaacgattct ctaagatgct
     7501 gaagcaccaa gtgaatcggt tccgtactat agtggattaa atttaaacca gtacggcgtt
     7561 gcctcgcctt gccgtactat ttgtactgtc tgcggctctc cgccttgtcc tgatttttgt
     7621 taatccgcta taccaaaact caaatcaagc cgtccggagg cggctcaaaa aaaacggtac
     7681 ttcgcagcag aagtaccgtt tatcgggatt tcaggtttta ttcttcgggt cgttcgccgt
     7741 cggtttcgtc ctgcgtccct tcggtgatgt gcatttctac gccgttgagg gcgcggattt
     7801 ttgcgtcgat ttcgtcggaa atttcggggt tttctttcag ccagacgcgg acgttgtctt
     7861 tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc gccggatttg ttgatgatgt
     7921 cgtttttcac gccgatgtcg atcaattcgc cttcccaact gatgccttct ccgtagagga
     7981 tgtcaaactc tgcctgacgg aacggggggg cgactttgtt tttgatgact ttgacgcggg
     8041 tttcgttgcc caatacttct tcgccttttt tgatggatcc ggtgcggcgg atgtcgaggc
     8101 ggacggaaga atagaatttc agcgcgttgc cgccggtcgt ggtctcgggg ctgccgaaca
     8161 ttacgccgat cttcattcgg atttggttga tgaacacaac cagcgtattg gtttttttga
     8221 tgtgtccggt cagtttgcgc aaagcctgac tcatcaggcg cgcctgcagt ccgacgtggc
     8281 tgtcccccat atcgccttcg atttcggctt tggggacgag tgcggctacg gaatcgacga
     8341 ctaccatatc tatgccgccc gaacggacga gcgtgtcgca gatttccaaa gcctgttcgc
     8401 cggtatcggg ctgggacagg taaagctctt cgacttttac gccgagtttg cgggcgtaaa
     8461 cgggatcgaa ggcgtgttcg gcatcgacaa aggcgcacac gccgccgttt ttctggcatt
     8521 gggcgacggc ttcgaggcag agtgtggttt tgccggagga ttcggggccg aagatttcga
     8581 cgatgcgccc gcgcggcagg ccgccgactc cgagggcgag gtctaatccg agcgatccgg
     8641 tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc cattttcatg atggcgcctt
     8701 tgccgaaact tttttcgatt tgcgccagtg cggcggcaag ggctttgctt ttgtcgtctg
     8761 acatgggggt tactccggaa caaatgcggt atgtgggctg cggcgcaaca cgggctgcgg
     8821 cgcggaatat gaatcggtcg gtcattatcg catgtttgcc tttcgggggc taccatccgc
     8881 ttcagaccga atgccgtctg aaacattgtc cctcctttgc tataatggcg cccttacttt
     8941 tcccgccagc cgcttgaacc gccatgcaca ccagcctgac cgtcaaaaat accgttatcg
     9001 gaagcgggcg caccaaaatc gccgtgccgc ttgtcgcccg agatgccgcc gatttatcct
     9061 ccgtgctgtc ccaaatcaaa aacctgccct tcgatatcgt cgagttccgc gccgactttt
     9121 tggaatgcgc gggcagcatc ggcgaagtat tgcgccacac gcaggccgtc cgcgacgcgc
     9181 tgcccgacaa accgctgctg tttacgttca gacggcattg cgaaggcggc tcgttcccgt
     9241 gttcggacga ttattatttt gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca
     9301 ttatcgatat cgagctgttt tccggcgaaa ccgccgtccg ccgcgccgtg gcaaatgctc
     9361 aaaaaaacgg catcgccgcc ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag
     9421 aagaaatcgt atgccgtctg aaacagatgg aggactgcgg cgcggacatc tgcaaaattg
     9481 cggtgatgcc gcaaagctcg gaagatgtgc tgactttgct ttccgccacg ctcgaagcga
     9541 aacggcttgt cgccaaaccg gttattacga tgtcgatggg gcagacgggt gcggtcagcc
     9601 ggcttgccgg acaggtgttc ggctcaagca tcacgttcgg ttcgggaacg caaaactccg
     9661 cgccggggca aatcggcgta tccgccctcc gtgcggcact cgactgcctc gaaagcggcg
     9721 cagactgatt tcagacggca tcaaaacatg atgaaactca atgcccaaca gctcgaagcc
     9781 gtccgctacc tcggcggccc cctgttcgtc cttgccggcg caggcagcgg caaaaccggc
     9841 gtgattacgc aaaaaatcaa gcatttgatt gtcaatgtcg gctatctgcc gcataccgtc
     9901 gccgcgatta cctttaccaa caaagccgct gcggaaatgc aggagcgcgt cgccaaaatg
     9961 ctgcccaaac cgcaaacgcg cgggctgacg atttgcacgt tccattcttt gggcatgaag
    10021 attctgcgcg aagaggcgaa ccatatcggt tacaaaaaaa acttctccat tctcgactct
    10081 accgacagcg ccaaaatcat cggcgaactc ttaggcggca cgggaaaaga agccgtattc
    10141 aaggcgcagc accagatttc cctttggaaa aacgatttaa aaacgcctga agatgtcgtt
    10201 cagacggcct ctaatgtgtg ggaacaacaa accgcgcgcg tgtatgcaag ttatcaggaa
    10261 accctgcaaa gctatcaggc agtagatttt gacgacttaa tccgcctgcc tgccgtgctg
    10321 ttgcagcaaa acagtgaagt gcgcaacaaa tggcagcggc ggctgcgtta tctgttggtt
    10381 gacgaatgcc aagatacgaa tacctgccaa tttgcgctga tgaaactctt gacaggcgcg
    10441 gaagggatgt ttaccgccgt cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg
    10501 aacatggaaa acctgcgcaa aatgcaggaa aactatccgc agatgaaggt catcaaactg
    10561 gagcaaaact accgctccac cgcgcggatt ctcaaaattg ccaacaaagt catcgaaaac
    10621 aaccccaagc tgtttaccaa aaaactttgg tcgcaattgg gcgaaggcga gccggttaaa
    10681 gtcgttgcct gccaaaacga gcagcacgaa gccgattggg tcgtcagcca aatcgtcaaa
    10741 caaaaactca tcggcggcga caaaacccga tacgccgatt ttgccgtgtt ataccgcggc
    10801 aagcatcagg cacggatttt cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc
    10861 tccggcggac aaagcttttt cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg
    10921 ctgcttgcca accccaacga cgatcccgcc tttctgcgcg ccgttaccac gccaaaacgc
    10981 ggcatcggcg atgtaacgct gggcaaactc aacgcctacg cgcacgaaca cgaatgcagc
    11041 ctgtatgaag ccgcacaaaa cgaagaagcc cttgccacac tgaacaatac caaccgccaa
    11101 cacctgcaag cctttatgga tatgttcggc aactaccgcg ccaaagccga aatcgacgaa
    11161 gcgggcgagt tcatcaacag cctgctcgaa gaaatcgact atgaaaacca tttgatgcaa
    11221 aacgaagaag gcaaagccgg cgaaatcaaa tggcgcaacg tcggcgagtt ggtatcgtgg
    11281 ttcgcacgaa aaggcgaacg ggacggcaaa aacgtcatcg aactcgccca aaccgtcgcc
    11341 ttgatgacgc ttttggaagg aaaagacgaa gaagaaaccg atgccgtctc gctatccacg
    11401 ctgcacgccg ccaaaggttt ggagtatccg tatgttttcc ttgtcggttg cgaagaaggt
    11461 gttttgccgc acaacgacag catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg
    11521 tacgtcggca tcacccgtgc caaacgccaa ctcacgctga cccactgtgt caaacgcaaa
    11581 aaacaaggca tatggcaatt ccccgaaccc agccgattca tagacgaaat gccgcaggaa
    11641 gatttgaaaa tcctggggcg caaaggcggc gaaccgattg tcagcaaaga agaaggcaga
    11701 cgtaaccttg ccgatataat cggaaggatc gacaacctaa aaaaaagcgg cccggcggat
    11761 taaaccggag ccgcaatgcc gtctgaaaga cgttcagacg gcatattttt tgaacggcgc
    11821 gcgtcaagcg gtttacgccc acaaatcctg ctgctgattt ttcggtacaa gcctgccgac
    11881 accgataccg atgaggcgga acgcgtcttc cgtctgcggc gggacgcgcg ccatcaacat
    11941 ttgcgcagcc tgcagcagag ctgcgcagtc gggcaatacg gaggaataag tcagcgtgcg
    12001 cgtgatgatg cggaaatcgt aggtcttcag cttgagcgtt acgctttggg cttcgacgtt
    12061 tttgcgcgtg atttgccgcc acaggtcttc ggcaagatgg ggcaggtgtc cggcagcctg
    12121 cccgagcggc aggtcttcgg gcagggtgat ttctgtggaa atttggaggc gttcgcgttc
    12181 ggctttgacg gggtgttcgt ccgtaccgcg cgccaaatca tagaggcggt agccgtagcg
    12241 tccgaaatga tttaagagct cgccgcgctc gaaacggcgc aaatcgcccg ccgtccgcat
    12301 acccagcgac tgcatttttt tcagcgttac cttgcccgct ccggggattt tgcccaaagg
    12361 cagggtttcc aaaaatgcca taactttgtg cggcggcaac acaaactgcc cgttcggctt
    12421 gcgccagtcg gaagcaattt tcgccagaaa tttgttcggc gcgatgcctg cggatgcggt
    12481 caaacccgtt tccgcaaaaa tggcggcacg gatttctttg gcaacttcgc cggcgtaagg
    12541 gatatttttg aaattacggg taacgtcaag ataggcttcg tccggcgaca agggttcgat
    12601 taaatcggta taacgcctga atacggcgtg aatctgcgcg gaaacctgac ggtacaaatc
    12661 gaaatgcgga ggcacataca ccgcttgcgg acacagcctt ttcaccgttg ccaccgacat
    12721 cgcggaatgc agcccgaact gccgtgcctc atacgatgcg gcgcaaatca ccgaacgcgc
    12781 gccctcccac gcgacgacca ccggccgccc tttcaaatgc ggctgctcgc gtagttctac
    12841 cgatgcgtag aacgcgtcca tgtcgatgtg gatgattttg cgtaaagaca tcggctctgc
    12901 cgaggataaa agggatattt tactgcgggc atcggacaaa atccaaatat actaacaggt
    12961 taaatacaaa agagttggct ggattaattt tgaaactcga taaaaataat ggtttttatc
    13021 aagcagaaag aaacagtaat ggaaattatg atatcgtaag aaaaaattat ctgaaaaata
    13081 ttaccaaaat acaatcaaat atattgattt acttagataa taccagtacc ggatttaaaa
    13141 ttcaacagag gagaaaacaa atcagagcac aaatttcagc cagacaatgg agaagataaa
    13201 agatgaataa aaatataatt tatatatttt ctttattaat cactattgtt attttcttta
    13261 tatttgaaaa gaatgtaata agcaaaataa gttttgatta taataaaaaa gaattcttaa
    13321 ttagtgatat aacgaacttt aactgggatc atgtaaaact gtatataatc aattcagatt
    13381 tccaaaaaat tgttttttat gataaaggta agatagtttt tgaggaatta atagaactag
    13441 ataggaaagg gaaagtttta cctcaatatc tatttgattc atatttgaaa aatgtttaat
    13501 attacgaatg tgattacaag aatggcaaga tgcaactttt aaaaaagaaa aattacattc
    13561 ttttgataga tatttttatt attataaacc tataaactgt aggccgaaac ttttgtaaaa
    13621 ctgtccatac tcattaccgc ctagtcggta atgcattttt tagtagtatt tgaaaaagta
    13681 tgatgataaa aacggttaag cctaactttt tcataaaatc cagcagagta aggcagaaaa
    13741 aaacacccaa attggctata atcccgacaa acacactcaa ggacaacaac atggcagcct
    13801 cgcccgaagc aaaattcacc gaagaaaaga ttttgtgggt caaacaccac acgccgaaac
    13861 tcatcacttt cgccatcagc cgtcccgaat cctaccgttt taaagccgga cagttctccc
    13921 gactcggttt ctacgaaggt aaaggcttta tttggcgtgc ctattccgtc gtttccgcag
    13981 aatatgccga cacgctcgaa tattttgccg tactcatcca agacggcccc atgtcggccc
    14041 ttttcgccaa aatgcaacag ggcgacacca tcctgctcga taaaaatgcc actggctttc
    14101 tcctgcccga acgcttcccc gacggcaagg atttggtgat gctctgtacc ggctcgggca
    14161 tcgccccctt cctttccatt ctcgaacaac ccgaaatccg gcagcgtttc gataccgtca
    14221 acctgataca ttccgtatct tttcccgaag aattgatttt caacgaccgg ctggccgcat
    14281 tgtccgaaca tcccctggta ggcgaatacg gacactcgtt ccgtttcgtc cctgttacca
    14341 cccgtgccgc caacccctcg ggcttaagcg gaaaacgcat tccggaactc ttaaaaaaca
    14401 acagcatcga acaggcgctg cataccaagc ttaccccgga atccacacgg tttatgattt
    14461 gcggtaaccc ggaaatggtc aaagacactt tccaaacgct gctcgatatg ggttacgcca
    14521 tgcaccgcaa ccgcattccc ggtcaaatca tgatggaaaa cggcttctaa aaacgaacgc
    14581 tcccaacccc tacccacgcc cacacacaaa acaaaataaa aaacagcgat atacaaaaac
    14641 caatttgcaa aaacagacct cacgcctata ctgccactca cagcaagcaa cacaaaaaag
    14701 cgggtacaaa ttagggtaca aaacacaagc ccgaacacag aagcacccca accaaaccca
    14761 aaggcaaagg caaacaaaat ggcggcacac aacaaactga cccaaaaaca aatagaagca
    14821 gccaaggcag acggcaaaca aagcaagctg gccgacggcg gcggcttata tctgctgctg
    14881 caccccaacg gcagcaaata ctggcggatg cgctaccggc acggcggatg cgaaaaaacc
    14941 ctagccttgg gcgtatatcc cgccgtcagc ctcaagcagg cccgcgaact tgcccgcgcc
    15001 gcccgggcgc agaccgccgc cggcatcgac cccgtcgccg aacaccaccg cacccgtccc
    15061 ggcagcgggc gcagccttcc cgaaatcgcc cgcgcgtggt acggcagccg tcaagggcaa
    15121 cgcgccgaca acacactgga aggcgaccgc cgttcactcg cctacctgac cgactactac
    15181 aaagacaaca ccgacatcaa cgacatcacc accgccggcg tatcaaaatt tatcgaacac
    15241 ctcaaccgca tcaacatccc cgcctgcgcc cggcgcaccg tccaaatcct cgcccaaata
    15301 tgggactatg ccgtccagcg cggcatcatt accgacgaac ggcgcaaccc cgcaaccacc
    15361 gcccgcccac tgctaaacac aagcaaaccc aaaccccagc cccacatccg ccccgacgag
    15421 ttgcccgact tttaccgcac cctccaaacc gcccacgacc tgcacccgta cgcccgcccc
    15481 ctgctgctgc ttgccgccct gaccgtcccc cgtccgtccg cactgctttc cgcccgctgg
    15541 caagacatcg acctcaccgc ccggttatgg cacatccccg ccgccgacat gaagaccaaa
    15601 caccccttta ccgtcccact gtcggactgg gcagtagaaa tactccgaga gctgcacaca
    15661 cagaccggcg acaacatcca ccttttccca ggcatccgcc cacggcgcaa acccgccccc
    15721 catcccgacc acatcagcat caaattcgcc cacaacgcca tccgacgact gggctatgac
    15781 ggcagcaccc cggcaaatcc aaacacacca tgcacggatt ccgcgacctc ttcaccaaca
    15841 tcagcctgac cgccggaaaa gacaccctca caacagacct cgcccttggc cacatcagcc
    15901 gcgccgccct ccaacgcgcc ggcttctcaa gcctgcacca ctacctgacc gccgacagct
    15961 accgcctcca agagcggcgc gaactcgccg aatggtacgg ccgccgccac cgccaagcat
    16021 acgaagccgc cgcccaaaac acttgcaata aaacctaaaa ggtattaaca tacatacatg
    16081 gcagacatcc tgccatccga caagcggcgg caaccgccaa aaacgaaagg aaatccaata
    16141 aacataatcg aacaagaaac aaaacgcacc gagttaagaa aaatagatgc cgaaatcgcc
    16201 aaaatcattg ccgacgcgca caaaatcaac gccgaatccg taaaaatagc ccaagagtcc
    16261 cgctggtatc ccatgatggc ggccaccggt ctggttaccg ccatcgccgc cgtgttggca
    16321 ctgatattca aatttgcctg accacaagcc ccgcgagggg ctttatccct caacaaaatg
    16381 aacagagccg cactattcaa ccgctacccc gaatggatta tcgggcaaga cggcgcaagc
    16441 cgtttcatta cccattgccg ctatccccgc ctgattgcca aaatccacag gcagacagac
    16501 ggcgaatgcc cgggcgggca ttaccgccat tccgaaaacg gcatcaccct ttacgatttt
    16561 atttttttcg gcggcaaacc ggcagacgaa gcccgatttg ccgccgtcct taccgaaacc
    16621 tgccgccggg cagtcaaaaa aa
//
LOCUS       DAXDSD010000047        16403 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXDSD010000047 DAXDSD010000000
VERSION     DAXDSD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16403
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.047"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            319..1776
                     /locus_tag="ACFB2Q_001163"
     CDS             319..1776
                     /locus_tag="ACFB2Q_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC0918195.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSRILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
     gene            complement(2105..2464)
                     /gene="rnpB"
                     /locus_tag="ACFB2Q_001164"
     ncRNA           complement(2105..2464)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFB2Q_001164"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2543..3646)
                     /gene="prfB"
                     /locus_tag="ACFB2Q_001165"
     CDS             complement(2543..3646)
                     /gene="prfB"
                     /locus_tag="ACFB2Q_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC0918196.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(3749..4642)
                     /locus_tag="ACFB2Q_001166"
     CDS             complement(3749..4642)
                     /locus_tag="ACFB2Q_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC0918197.1"
                     /translation="MVTFSKIRSFLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVA
                     LALGGGASKGFAHIGIVKVLKENGIPVKVVTGTSAGSIVGSLLASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHKYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNVGQGFFSYLDQTLNVMSVSVLQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            4983..5432
                     /locus_tag="ACFB2Q_001167"
     CDS             4983..5432
                     /locus_tag="ACFB2Q_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC0918198.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNFAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPKCRLKQD"
     gene            5506..5814
                     /locus_tag="ACFB2Q_001168"
     CDS             5506..5814
                     /locus_tag="ACFB2Q_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918199.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAAHKPASNATGVQKSAQGSCGA
                     SKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            5932..6774
                     /locus_tag="ACFB2Q_001169"
     CDS             5932..6774
                     /locus_tag="ACFB2Q_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC0918200.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELAYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            6764..7510
                     /locus_tag="ACFB2Q_001170"
     CDS             6764..7510
                     /locus_tag="ACFB2Q_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC0918201.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            7532..8119
                     /locus_tag="ACFB2Q_001171"
     CDS             7532..8119
                     /locus_tag="ACFB2Q_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC0918202.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            8125..8304
                     /locus_tag="ACFB2Q_001172"
     CDS             8125..8304
                     /locus_tag="ACFB2Q_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC0918203.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            8536..8976
                     /locus_tag="ACFB2Q_001173"
     CDS             8536..8976
                     /locus_tag="ACFB2Q_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC0918204.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(9128..9643)
                     /locus_tag="ACFB2Q_001174"
     CDS             complement(9128..9643)
                     /locus_tag="ACFB2Q_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918205.1"
                     /translation="MKTFLHTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTSDYFLEAVLVTGRSRECKKCFIKIMRASSFVYQ
                     WNNLRLNSEIV"
     gene            complement(9658..10260)
                     /locus_tag="ACFB2Q_001175"
     CDS             complement(9658..10260)
                     /locus_tag="ACFB2Q_001175"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC0918206.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPSENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(10294..11565)
                     /gene="purD"
                     /locus_tag="ACFB2Q_001176"
     CDS             complement(10294..11565)
                     /gene="purD"
                     /locus_tag="ACFB2Q_001176"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC0918207.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            11702..12040
                     /locus_tag="ACFB2Q_001177"
     CDS             11702..12040
                     /locus_tag="ACFB2Q_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC0918208.1"
                     /translation="MKKEQANTQKRIDEIQSKYREWCHLLLQLEENIRRWKYAVALIR
                     DMDNFYTHEYQVRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSV
                     DTLDRYTQEN"
     gene            complement(12092..12703)
                     /locus_tag="ACFB2Q_001178"
     CDS             complement(12092..12703)
                     /locus_tag="ACFB2Q_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC0918209.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCRLTVSLSLVNYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(12766..13701)
                     /locus_tag="ACFB2Q_001179"
     CDS             complement(12766..13701)
                     /locus_tag="ACFB2Q_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC0918210.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            complement(13712..14461)
                     /locus_tag="ACFB2Q_001180"
     CDS             complement(13712..14461)
                     /locus_tag="ACFB2Q_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC0918211.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQTGVKVASVAELVEKLKNEAKVI"
     gene            complement(14687..15655)
                     /gene="waaC"
                     /locus_tag="ACFB2Q_001181"
     CDS             complement(14687..15655)
                     /gene="waaC"
                     /locus_tag="ACFB2Q_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC0918212.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            complement(15692..16327)
                     /locus_tag="ACFB2Q_001182"
     CDS             complement(15692..16327)
                     /locus_tag="ACFB2Q_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC0918213.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMAAKWLVGNPVDMLKPTGLSDADLTWVAHAMVGTRGYELLDGLPSVKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTNMVIVGGLATDYCVKTTVLQL
                     LKGGRWQVIVNEAACRGIAPDTIEAAWQEMRSSGAIILKNAEKIKKYINNQ"
BASE COUNT         4277 a         4467 c         3428 g         4231 t
ORIGIN      
        1 aagttaatcc accataatgc gcccttcggc gtggcggata tataaggaag tgattttcca
       61 tctaagtaaa aaccgcccta tcggataagc cattgacaga aaaggattta cccgcgccgt
      121 atcggaacac gtcctctaaa atacaatcca ttgaattgaa acaaatataa aaacatccgc
      181 ccgcgaaaaa cggcagcgcg tcgtttgaca aagaatgaaa atatcggtta aaaaccgata
      241 ttttcataca aaaaaacacc gccgccgtcc gcatccgttt cagacggtat tgagagaaaa
      301 tcttttagga gaacctttat gtcccggcat cccgcccgca ccggagaaaa aacatttttc
      361 ggccacccct tccagctttc caccctcttc catatcgaat tgtgggaacg tttttcattt
      421 tacggaatgc agggcatcct gctgatttac ctctactaca ccgccgacaa aggcggcttg
      481 ggcatagaca aaaccctcgc aggcggcatt gtcggcgcat acagcggcag cgtgtacctg
      541 tccaccattt tgggcgcgtg gtttgccgac cgcgtatggg gtgcggaaaa aaccctcttc
      601 ctctcgggca tcgtcgtaat gctcgggcac atcgtccttg ccgccgcacc gggcctgtac
      661 ggccttctaa tcgggctgat attcatcgca ttgggcagcg gcggcgtgaa atctacggcc
      721 agttctatgg tgggcgcatt atacgaacag gacgaaatgc gcccgctgcg cgatgcggga
      781 ttttccattt tctacatcgc catcaatatc ggcggcttct taggcccctt attgaccgga
      841 ctgcttcaag aaaacatcgg tttccattat ggtttcggcg cggcggcggt cggtatggca
      901 ttcggcttgt ggcgttattc cttgggacgt aaaaacctgc cccaccccac cgtcccccat
      961 ccgctttcaa aaggacaggg caaaactgcg gccgccgtcg gcatcaccct catcgccgca
     1021 cttgcaaccg ccatcaaaac cgggcttgtc aacctcgaca atttctcccg catcctctta
     1081 tctaccgtca tccttgccgt catcgcctat ttcgcccgcc tgctgaccaa cccccgcgtc
     1141 agttccgaca acaaacggca catcatcgcc tacatcccgc ttttcctgac catctgtatg
     1201 ttttgggccg tctggtttca gatttacacc gtggcaaccg tctatttcga cgaaaccgtc
     1261 aaccgcacca tcggttcgtt taccgtaccc gtcgcctgga aagattctat gcaaagcctg
     1321 tgggtcatcc tgttttccgg actcatggcg gcaatgtgga caaaaatggg gcgcaaacaa
     1381 cccaaaaccc cgctgaaatt cgctatggcg gtatttgtta ccggcgcgtc gtttttggga
     1441 ttcgttccct ttatttcctc cggaacgccg atgcctattg cggttttcgc actgatcgtc
     1501 ctcgccatca cgataggcga actgatgatt tccccgattg cgctgtccat ctccaccaaa
     1561 atcgcaccgc ctttattcaa aacccaaatg gtcgccctta atttccttgc cttttcatta
     1621 ggcttcactt tgggcggcgt attgtttgaa aaaggctatc aggcgggcga cgaaatcggc
     1681 ttctaccggc tgctgttcta catcggcgca gccacaggct tcctgctgct cctgctcgtc
     1741 cccaaattga acaaaatgct cgaaggcaca gactaagtcc tgccccgatg cgccgatacc
     1801 gtctgaaccc ttcagacggc atttttccgc ataatgaaac caaaccgttt ccacccgaca
     1861 ggacaggctt tcccccccaa ccggaaggca gcctgccgat tgtcatttaa atacccgcct
     1921 gattccgtct gtttgcaagg gaaatagccg tttatttccg cttgggcgga aaccggtcct
     1981 attgagtaaa aggcattttg tccgactcat cagggctgca tcaacggaat ataaaaacac
     2041 agcctaaata aaaatgccgt ccgaaccgta tttcaggttt cagacggcat ttgcgtgtcg
     2101 gatgcacacc ggacaggcgg taagccgggt tctgtctcgg acagtcattc ctctaggcat
     2161 accgttgccg gtatgctcaa gcaacctacc cgaacgctcg gcgggcagcg tcattgcgtt
     2221 ctgtttggtc ttgctccgaa tggggtttgg cctgccgcat attgttacca aatgcgcggt
     2281 gcgcccttac cgcacctttt cacccttgcc tgtgctgcca aagcagccat cggcggtttt
     2341 gctttctgtt ccactttccg tcgcgttacc gcgcccggcc gttaaccggc attctaccct
     2401 gcggagcccg gactttcctc cccgtatgcc ttacgcgata cgcggcgact gtctgcccgt
     2461 cccgtgtgcg gcgcggatta taacacgaaa cgcaaaaatg ccgtctgaaa cggtacaggt
     2521 ttcagacggc atacagccta aactacacac cctgtttcag gctggcttcg ataaagccgt
     2581 ccaagtcgcc gtccaatacg gctttggtgt tgccgacttc gtagcctgta cgcaagtctt
     2641 tgatacgtga ggaatccaaa acatacgaac ggatttggct gccccaaccc acatcggatt
     2701 taccttcttc caatgcctgt ttctcttcgt tgcgtttgcg catttccaat tcatacagtt
     2761 tggatttcaa catttccatc gcggcggctt tgttggcgtg ttgagaacgg tcgttttgac
     2821 attgcaccac aatccccgtc ggctcgtggg taatacgcac ggcggagtcg gttttgttga
     2881 tgtgctgacc gcccgcaccc gatgcgcgat aggtgtcgat gcgcaaatcg gcggggttga
     2941 tttcgatttc gatggaatcg tcgatttcag ggtaaacgaa cacggaggca aacgaggtat
     3001 ggcgtttgtt gttcgaatca aacggcgagt agcgcaccag acggtgaacg ccggtttcgg
     3061 tacgcagcaa accataagcg tattcgcctt ccactcggat ggtggcgcgg ttgatgcctg
     3121 cgatttcgcc gtcgtcttcc tcaaggattt cgattttgaa gcctttgcgc tcggcatagc
     3181 ggctgtacat acggaacagc atccccgccc agtcttccgc ttcggtaccg cccgcgccgg
     3241 cggtgatgtc gataaagcag ttgttcgggt cggcgggctg gttgaacatc cgtttgaact
     3301 ccaaatccgc catctgtttt tccagccccg ccacgtcttc cttcacggcg gcaaaacctt
     3361 cttcgtcgtt ttcttcgacg gccatttcaa tcagcatacg gttgtcttcg atacccgaag
     3421 cgatgttgtc gagcgtcagc acgatgcctt caaggatttt gctctctttg ccgatttctt
     3481 gggcacgttt cgggtcgttc caaagctcgg ggtcttcgga aagcccgatg acttcttcca
     3541 atcggtcttt cttgccctga taatccatat aaacgcggat gtcttcgcta cgcttctcca
     3601 aatcattcag ggtgttgttg agctggttga ttacttcggc ttccatgatt ctttcgttct
     3661 ttcaaaattt taggggcgta ttgtacggga ttcgggtatt tttttctatg gataaagcct
     3721 tctggaaata cgttcagacg gcatagcgtc aataacggta tgccgccagt ttgcgtttga
     3781 tttcaggcaa tgcggcacgt gctgcctcct cgcccaaccg gatggcgcgc tttttctgat
     3841 cgaatccgcc gactgcaccc aaatccaaaa cctgcggttt gataaccaca tccgcctgcc
     3901 ccaactcgtt ttgcaacacg gaaacgctca tcacgttcag cgtctgatcg agataagaga
     3961 agaaaccttg accgacattt ttgctcggac gtgcggaaat atcgacggca atcacgaaat
     4021 tcgccccctg ccgccgagcg gcactgacgg gcacgggctg cgacagaccg ccgtcaacat
     4081 atttgtgcct gccgatgatg actggctgga acacattggg aatggcggcg gaagcacgaa
     4141 ccgcctgccc ggcattccct tgattgaaag cgacggcctt gccggtttca aaatcagtgg
     4201 caacggcggc aaatttgatg ggaaactgct gaatctgcct gccgccgact tttcggttga
     4261 tgtaattttg cagcttttcg cctttgataa aaccactggt ggacaaggtt aaatcgacta
     4321 aatcggtttt acctaaaatc tcggcttcca attcgaggcg gtcgggcgac atacccgatg
     4381 ccaaaaggct gccgactatc gaacctgccg atgtgccggt aaccaccttc acaggaatac
     4441 cattttcttt caaaacctta acaattccta tatgggcaaa tcctttagat gcgccgccac
     4501 cgagtgccaa agcgaccact gcggcgggtt tggcggtttg caccggcttg cgggcggcat
     4561 tgtttcccgc cgtaccgcag gcggcaagca acgcggcggc ggcgattgcc aaaaatgatc
     4621 tgatttttga aaacgttacc atattttcca ttcctttaca tatcgcaccc cgtcaaaggg
     4681 agattgcttt tcttaacaat ctcctttgac agccaagcga aaggggcttt gttaagtcat
     4741 catcaaaatt aatattttct ttttttcctt tacggaaatt gtatttgaag gcataccgtc
     4801 caaggcggga attatctcac aacaccgccg ttatccaaat atcccgcctt tttccctttc
     4861 tttccgtcaa aatactttct ttttatattc attaacttgt taaattatta gctgacgggt
     4921 gtcagttttt ccgacaaaat ccgtctaacg gggtatcaac aggaacaaaa acaggaacac
     4981 ttatgaaaat cggaacaact tggcagacgg catccgctat gctggttttg cgtctgtttg
     5041 ccgcatatga atttttggaa tcgggtttgc aaaaatggaa cggggagaat tggttttccg
     5101 aaatcaacga tcagtttcca ttcccgttca acttgctgcc ggacgcgtta aactggaatt
     5161 tcgccatgta tgcggagctt ttgctgcccg tactgttgct tttgggtttg gcaacgcgcc
     5221 tgtcggcatt ggggctgatg gtcgttaccg ccgtcgcttg ggctgcggtt cacgccggtt
     5281 cgggttacaa tgtctgcgac aacggttata aaatggcttt aatttatatc gtggtattaa
     5341 tcccgctgct tttccagggt gcgggcggat ggtcgctgga tacgctgctg aaaaaacggt
     5401 tttgccccaa atgccgtctg aaacaagatt gattcagtcg tggaatccga ctttaaacat
     5461 tccaacctta tctcgttaac ttgatattct gaaaaggaaa tagaaatgaa caaaaatatt
     5521 gctgccgcac tcgccggtgc tttatccctg tctttggccg ccggcgccgt tgccgcccac
     5581 aaaccggcaa gcaacgcaac aggcgttcaa aaatccgccc aaggctcttg cggcgcatcc
     5641 aaatctgccg aaggttcgtg cggcgcggct gcttctaaag caggcgaagg caaatgcggc
     5701 gagggcaaat gcggtgcaac tgtaaaaaaa gcccacaaac acaccaaagc atctaaagcc
     5761 aaagccaaat ctgccgaagg caaatgcggc gaaggcaaat gcggttctaa ataatccctc
     5821 cccttcaaac caagccgcgt ttttcagtaa aatgcggctt ttttaacggc aaacaaagat
     5881 ttttttaaac aagcacatta ttcttttgtg ccatccgaac cgggtaaaaa tatgattcaa
     5941 cacgcaggct tgggctaccg ccgcgacttg gcggaagact ttctctcgct ttccgaaaac
     6001 agcccgatat gctttatcga agccgcaccg gaaaactggc tgaaaatggg cggcagggcg
     6061 cgcaaacagt ttgaccgtgt ggcggaacgg ctgccgctgg cgttgcacgg attatctatg
     6121 tcgctgggcg gacaagcccc gctggatacc gatttgatag acggcatcaa agaaatgatg
     6181 tgccgttatg actgcacgtt tttctccgat catttgagct actgccacga cggcggtcat
     6241 ctttacgatt tgttgccgct gcctttcact gaagaaatgg tgcatcatac ggcgcggcgt
     6301 atccgcgaag tgcaagaccg tttgggctgc cgcatcgccg tggaaaacac gtcctactac
     6361 ctgcattctc cgcttgccga gatgaacgag gtcgagttcc tcaacgcggt tgcccgtgaa
     6421 gccgactgcg gtattcattt ggatgtgaac aatatttacg tcaacgccgt caatcacggc
     6481 ctgctgtcgc ctgaggcctt tttagagaat gtggacgcag ggcgcgtgtg ctacatccac
     6541 atcgccggac acgatgccga aacccccgag cttttgattg atacccacgg cgcggcggtt
     6601 ttaccgaccg tttgggactt gctcgaactc gcctacacca agcttcccac catccctccc
     6661 accctgttgg aacgcgattt caatttccca ccttttgccg aactcgaagc cgaagttgcc
     6721 aaaattgccg attatcaaac gcgtgccgga aaggaatacc gccgtgcagc ctgaaacctc
     6781 cgcccaatac cagcaccgtt tctcccaagc catacgcggg ggcgaagccg cagacggtct
     6841 gccgcaagac cgattgaacg tctatatccg cctgatacgc aacaatatcc acagctttat
     6901 cgaccgctgc tataccgaaa cgcggcaata ctttgacagc aaagaatgga gccgtctgaa
     6961 agaaggtttc gtccgcgacg cgcgtgccca aacgccctat tttcaagaaa tccccggcga
     7021 gttcctacaa tattgccaaa gcctgccgct ttcagacggc attttggcac tgatggattt
     7081 tgaatatacc caattgctgg cagaagttgc acaaattccg gatattcccg acattcatta
     7141 ttcaaatgac agcaaataca cgccctcccc tgcggccttt atccggcaat atcgatacga
     7201 tgttacccat gatttgcagg aagcggaaac agccttgtta atatggcgaa acgccgaaga
     7261 tgatgtgatg taccaaacat tggacggctt cgatatgatg ctgctggaga taatgggttc
     7321 ctccgcgctt tcgtttgaca ccctcgccca aacccttgtc gaatttatgc ctaaagccga
     7381 taattggaaa aatattttgc ttgggaaatg gtcaggctgg attgaacaaa ggattatcat
     7441 cccctccttg tccgccatat ccgaaaatat ggaaggcaat tccccgagcc aaaaccatct
     7501 atccgcataa aattaccttg ttcccgatac tatgccgcta cccgacctga ccgatgccga
     7561 attaatggag tcgcgcaaac tgcttctgca ttttgcgcga ctccagttgc ccgaccaccc
     7621 tgatttggct gaagatttag tgcaggaaac attgctgtcc gcatacagcg caggcgacag
     7681 ttttcaaggc agggcacttg tcaacagctg gctttttgcc atattgaaaa acaaaattat
     7741 tgacgcatta cgtcaaatcg ggaggcagag gaaagtcttt accgcactgg atgacgagct
     7801 gctggatgaa gcatttgaaa gccatttttc ccaaaacggg cattggacgc cggaagggca
     7861 gccgcaacat tggaacactc cggaaaaatc attaaataac aacgaattcc aaaaaattct
     7921 gcaaagctgc ctatacaacc tgcctgaaaa caccgcacgg gtattcaccc tgaaggaaat
     7981 actcggtttt tcatccaacg aaatacaaca aatgtgcggt atcagcacgt ccaactacca
     8041 caccattatg caccgcgccc gagaatcatt gcgccaatgc ctgcaaatca aatggttcaa
     8101 ccaagaaaac ccgaagtaaa cgttatgaaa aaatgccgcg atattgccct gcttctttcc
     8161 aaacatcagg accgggaaac caccccgggc gagaagattt ccatatatat gcacctgctg
     8221 ttctgcccgc attgccggga atataaaaga cagcttcaaa ccatcaaaat atcactggca
     8281 aaaacaacca gaacttcaaa ataaatgccg tctgaaaggg cttcagacgg cataagctga
     8341 cggaaacaaa tcaaaccgat ttactgttat cggcagttca tccataatac actcttcaaa
     8401 agcagcatat tcctccatac ggaatatata aatacgtaaa acacgaaggc tgcatcaatt
     8461 tcccatattt gcctcatttc acagacggca ttacccctcc ggcccaaccc gttctttctg
     8521 aatgagcgga tttcaatgat taaggaaacc ctaatgcgcc caatcttcct atctttcgtt
     8581 ttactcccta ttttgataac cgcctgcagc acaccggaca agtctgcccg atgggaaaat
     8641 atcggcacaa tctcaaacgg caatattcat acatatatta ataaagacag tgtgagaaaa
     8701 aacggaaatc tgatgatttt ccaagataaa aaagttgtta ccaatctgaa acaagaacgt
     8761 tttgccaaca cccccgcata caagactgcc attgccgagt gggaaatcca ctgcaacaac
     8821 aaaacatacc gcttaagttc gctacagtta tttgatacaa aaaacacgga aatttccaca
     8881 caaaactaca cagcctcttc cctccgcccg atgagcatcc tgtccgggac attaactgaa
     8941 aaacaatatg aaaccgtatg cgggaaaaaa ctctgatttc aacttataca caaatttatc
     9001 cataagcctt attataaaaa tgccgtctgg aatactgaaa tatcagcatt tcagacgaca
     9061 ttttgccatt ccctgaaatt atccacatta tttttttaaa ccggcttcca tccgaaatat
     9121 aaacccgcta tacaatctca ctattcaaac gtaaattgtt ccattgatac acaaaactgc
     9181 ttgccctcat aattttgata aagcattttt tacattcccg gctccgtccc gtaaccaaca
     9241 cagcctctaa aaaataatcg cttgttttga caccgataca ttcatacaga tacagaaatt
     9301 caagaaaata gccttgtgca ttgatcattt caagcaattc agaaaaatca agaaattttc
     9361 tgactgtaaa caaacgtttc cctaaaaaga cgatgtcttc aaaaatatcg accaaatagt
     9421 cgtcttcggc gtttctattt tcatgggtta aaacaacacg ggaaaaatct gttttcagat
     9481 gcttgcccgc ttgattgttc ggattattgt ccagaacgac aaaaccgtcc tcaaaatcaa
     9541 agcagacgtt gcgtccttct acctttatcg cagtgcgata acaatcatgt agagaaatac
     9601 catccaaaaa attttttctt tgtgtatgca aaaaagtttt cactcaagca tccatatcta
     9661 acgcaaacgt ttacctgttt ccccatcgat aatctgactc ggcgatttct gcctgccgat
     9721 tctcccaccg acaatccaca catcgcgtcc gaactgcctt ctgacttccc tctccgtccg
     9781 acacgcgcgt ttacccgccc gattacatga agtggaaacc aaaggcatac ccaatgcctg
     9841 acacaaacgc cttgcacctg tatgatcggg cactctgacc gcaagcttgc tgcgcctctt
     9901 cccgcgcaac gcaggtaata cgccagattt agccaacaat aggaaagtct taggagcagg
     9961 ccattcgttt ctaagcatgg tttgaatgtt ttcagacggc atttgaagta aaggctgcaa
    10021 ttgttcaagt tgattcccga tgacaatcat acccttgtgt tgcggccttt ttttcaaatg
    10081 cgccaactta ccgagtgctt tggctaatgt cggaagacac cccaagccat aacaagattc
    10141 ggtcggataa gcaaccaaac cgcctttttt caaataaacg cttaacttac gttgcgctga
    10201 tgctgcgata attctcgaaa ataacataat ataaaatgcc gtctgaagcg catcagtcat
    10261 acttggcttc agacggcatc atcctctttc taattaacgg ttaatcgctt tatcggcaat
    10321 gtctttacgg tattgcatcc cgtcgaagct gattttttcc aacgcgccat acgccttggc
    10381 tttcgcttgc gccacatcgt cgcccaatcc tacaacacac aatatgcgcc cgccgttggt
    10441 caacacgtcg cctttctcgt ttaccgttgt gccggcatgg aatactttgc cgattcggtt
    10501 gacgtcatcc aaaccggaaa taacatcgcc tttcttgggt gtttcggggt aattttgcgc
    10561 cgccagtacc acacccacgg cagtttgcgg attccattcc gctttgacgc tgtccagtct
    10621 gccgtctatt gccgcttcaa ccaaatccgc caaatcgctg tttagacggc tcataatcgg
    10681 ctgggtttcg ggatcgccga aacggcagtt aaactcaatc gtatagggtg caccgctttg
    10741 atcgatcatc aaacctgcgt acaaaaagcc ggtgaactcg tgcccctccg ctttcatacc
    10801 cgctacggtc ggcaaaataa tctcgttcat cgcacgctcg tacacagcag gcgttaccac
    10861 aggcgcggga ctgtacgcac ccataccgcc cgtattcggg cctttatctc cgtctaaaag
    10921 acgtttgtgg tctcgactgg tcgccatagg taatacatga ttgccatcaa ccataacgat
    10981 aaagctcgct tcttcgcctt gcagaaaatc ttcaatcaca acgcgttcgc cggcattgcc
    11041 cattttattg cccaacaaca tatcgtcaat cgcagcatgc gcttcatcca aagtcattgc
    11101 cacaatcacg cctttacctg ccaccaaacc atcggctttg ataacgatag gcgcaccttt
    11161 ctgattgacg taatcatgtg cggcatcggc attttcaaag gtttggtatt gcgcggtcgg
    11221 aatattgtat ttcaccataa atgctttggc gaaatcttta gaactttcca actgcgccgc
    11281 atattgtgtc ggaccaaata ttttcagccc tgcggcacga aaatcatcca caatacctgc
    11341 cgccaaaggc gcttcagggc cgacgacggt aaaaacaata ttttctttac ggcagaattc
    11401 aatcaaatcc tgatacgcgg tcaaggcaat gttttggagc ttggattcaa tcgccgtacc
    11461 ggcattaccg ggcgcaacaa atactgtttc cactttgggc gactgcgcca acttccaagc
    11521 cagcgcgtgt tcgcgaccgc cattaccgat aaccagcagt ttcatgccat ctccttgaca
    11581 aatatgtact tttaacgaaa actcgataca aaggaacttt tgtcccatct gaagaaattt
    11641 tagtagaatc aaacaaaaga ccacttcatt ccactctgca acctattcaa cttattcaca
    11701 aattaaaaaa gaacaagcaa acacgcaaaa acgtattgat gaaatccaaa gcaaataccg
    11761 cgaatggtgt catttactac tgcaactgga agaaaacatc cgccgttgga aatatgccgt
    11821 cgctttaatt cgcgatatgg acaatttcta tacccacgag tatcaggtgc gccatcagac
    11881 tattgaagac ggggtagaac tgaatttgca aacagaaggc gaatatagca ttatgagtga
    11941 agatgcatta tggaacgcgc cgggcgaatt ccatcaattg gcttggctat atttatgctc
    12001 cagcgtcgat accttagaca gatatacaca agaaaattaa tcagcgaaga ggttgcctga
    12061 aataccatta caaagcattt catacaacct ttcattcaaa aggcttttcc gtatttactt
    12121 caacctgccg tgtattcttc caagccgcaa cacaggcctc ataatttacc aacgacaaac
    12181 ttaccgtcaa tcggcaatcc aactgcaaat cctgctccaa tatatccgcc tgatattgtt
    12241 tggcaatgca tattgcttca ttcaaaaacg gatactcaca tttcagccaa acggtttttt
    12301 caatattctt ttcaaccact tccgcgatcg ccaacgcctg agccgttgcc tctttgtatg
    12361 cgtgaatcaa gccgggaacg cccaataaag caccaccgaa atagcggacg accacgacca
    12421 aaacatcagt gatacccacc gaatcaatct gtcccaaaat tggccgccca gcacttcctg
    12481 gtggctctcc atcatcgttg ccgcgaaact gcatcccgtt cacacctaaa cgataggcat
    12541 agcaccaatg ccgcgcttta taatgctctt cctttaacag attgaggtat tttttcacat
    12601 cagccaatgt ccgaatcgga taggcaaatg caataaaacg gctgccttta tctttaaact
    12661 cagcctgcgt cggggaagta atggttttat aagtcgtaat catgctgaaa tgttttcaga
    12721 cgacctcatt aataacaaag tcgcctgaaa gtttcacgtg aaacatcaat ttttcaataa
    12781 ttctattaat tgtggaacaa tttcaaataa atcgccaacc aatccgtaat cggctacatt
    12841 gaaaatcggc gcatcggcat ctttgttgat tgcaacaatc accttactgt cctgcatacc
    12901 ggcaacgtgt tgaatcgcac ctgaaatacc gattgcaaaa tagagctgcg gcgcaaccac
    12961 cttaccggtt tgtccgactt gagcatcgtt tggtgcatac tcggcatcaa ccgccgcacg
    13021 ggatgcaccg attgccgcac ctaaaacatc cgccaacggt gtcagcactt cactgaattt
    13081 ttccacacta cccaacgcac gaccaccgga aacaatcact tttgcctgag tcagttcggg
    13141 acgatcggaa tgggaaagtt gacggttaac aaaacgactc aggttttggg caggggttgt
    13201 tttaacatta attacctcag cattaccacc ttgcgccgcc actgcgtcaa aagccgtcac
    13261 acggaaggtc agcaccaatt tttctgaatc ggcttgcacg gtttcaaatg cattacctgc
    13321 ataaatcgga cgtacaaaag tcgtgttatc tacaatctcg gtcaaatcag aaatttgcgg
    13381 tacgtctaat aaggcggcta cgcggggcaa aaggttttta ccgaatgtgg ttgccgttgc
    13441 tgcaacatag cggtaatccg ccgccaattt gacaaccagc ggagccaact cttcagccaa
    13501 accttcagca taatgagcag catctgcaac caaaactttt tccactcccg ctacttgctt
    13561 cgcgaattcc acgacggacg atgcgccgtt tccgacaacc aataaatcga ccttgcccag
    13621 tttggcggca gcggcaacag catgcaaagt ggcaggattc aactgtttgt tgtcgtgttc
    13681 ggcaataatc aatacgctca tttcagcctc ctcaaatcac tttggcttcg tttttcaatt
    13741 tttcaaccaa ttcggcaacg cttgctactt ttacacctgt ctgacgcgcc ttaggttcgg
    13801 caaatttcac cgttttcaga cgaggcgaaa tatcggtagc caaatcggca gaatccagtt
    13861 tttccagagg ttttttcttt gccgccataa tattggggag tttgacaaag cgcggctcgt
    13921 tcaaacgcaa atctgcgctg atgactgcag gcagtttcaa cgcaatcgtt tcttcgccgc
    13981 catcaatttc gcgcacaatc tgtacttcat caccttcaat ttgtactttt gaaacaaatg
    14041 taccttgcgc cgcattcaac aaagctgcca gcatttgcgc cacttgattg gcatcgtcat
    14101 caatcgcttg tttgcccaaa aagaaaattt gcggattttc tttgtccgca acagctttca
    14161 gcagtttggc aactgccagc ggctctaatt tcacatcggt ttcaatatga atggcacggt
    14221 cggcacccat tgccaaagcg gtacgcaagg tttcttcgca tttcttttca cccaaagaaa
    14281 ccgctacaat ttcgcttact ttcccggctt ctttcaaacg gacggcttct tccacagcga
    14341 tttcatcaaa cgggttcatc gacattttga cattgccgat gtccacatcc gaaccatcgg
    14401 ctttcacacg gactttgacg ttgtagtcca ctacgcgctt tactgcgacc agtgctttca
    14461 ttgaattctc ctaaaaagaa cgctgctttc accatccagc gaaaccaaac cttcttcttt
    14521 ataaaatcaa atccgttttc cttaaaaacg aatttattta gcaatctttc ggataatttt
    14581 accgattata ccatttttaa agtatttact cagtctggcg gatatacatt cctgtatcta
    14641 ataaattgga aaatatcatg ccgccatatc agttttagac gaccctttag cctttatctg
    14701 ctgaaacaca atccatcagc gtttgataaa ccaaatctgc ggtcggaatc tgcccgatat
    14761 tgcccacatt ttttgcaata gccgaaactt gaacgcctgt tttaatcgga tcggtatcgg
    14821 tataaatgcc gaccacaggt ttttccaagg catttgccaa atgcagcaaa ccggtatcta
    14881 caccaactat tccaaccgcg tgtttcaaca gatacgccgc ctgtaataaa ttcattttag
    14941 cgcacactat ggtaaacggc agcccgtctg caatctgttc cgcacgcact ttttcagcct
    15001 cgtttcccca aggcagataa acattgcatt gttgtttttg attcagtttc tgcagcaact
    15061 cccgccaatt ttccataggc cataacttac tgtcccgact ggtcgcgtgc aaagccgcat
    15121 aatacggctg cgctaaattt ttcagacggc ttgcttcagg aacggacaaa ccaaatacct
    15181 gcgtctcagg cattgcatac ccaaacacct gggcaaacag atcacggttg cgccaaacgg
    15241 catttctacc tttcggtacg acatactttt tatcatacgc caaagcagcc accccctcac
    15301 gcgcactgtg tttatccaaa ccataaatag gggattttgc cattttggcg aaacacgcgc
    15361 ttttaatcag accctgactg tccaatacaa aatcaaatgc ttcctgcagc aaagcctgtt
    15421 tcagacgacc catctcccgc caagtttcag cctgaaagag atgtttgcgc cattgccgcc
    15481 acttcattac atggaccttt tttacaaacg gatgcaaccg tgcaataccc gcaaacccgg
    15541 cttcacatag ccaatgcagt tctacatcag agcattgtcg cgccaaatct tcgattgcgg
    15601 gcaaagtgtg aattaaatca cccatactgg acaaacggac aagcaaaatt ttcatatgta
    15661 gacagggtgt ttcacgtgaa acaattataa gttattgatt attaatatat tttttaattt
    15721 tttcggcgtt tttcaaaatt atcgcacccg aagaacgcat ttcctgccac gctgcttcaa
    15781 tggtatcggg cgcaataccc cgacaagccg cttcattcac gataacctgc caacgaccgc
    15841 ctttaagtaa ctgcaaaacc gttgttttaa cacaataatc cgtagccaag ccgccgacaa
    15901 tgaccatatt cgtattttga caacgcagcc actcaatcaa tccagtgctc agtttttcct
    15961 caatatcgtg aaaacacgcg ccgtaaggat gcaattcggg atcgacacct ttccaaacgc
    16021 aataatcgta ttccttgacg gaaggcagac cgtccaacaa ttcatagccg cgcgtaccga
    16081 ccatcgcatg agccacccaa gtcaaatccg catcggacaa acctgtcggc ttcaacatat
    16141 caacagggtt acccacaagc catttcgccg ccatatgatg cgcatctttc gtcatcacac
    16201 gcaaatccgc caaagcggct tgggcattta actcctcgac aatcaaatgc ccctcgttca
    16261 cgggcagttc gtcaggacac aaaggcgtaa aagttttttg tgcatcaaca tcaatggaaa
    16321 caatcatctc attacttctt catttaagat cgtcattctc tattttacga ataacaaaac
    16381 tcttaccttc tttatttaag gag
//
LOCUS       DAXDSD010000048        16241 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXDSD010000048 DAXDSD010000000
VERSION     DAXDSD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16241
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.048"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..181)
                     /locus_tag="ACFB2Q_001183"
     CDS             complement(<1..181)
                     /locus_tag="ACFB2Q_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918214.1"
                     /translation="MPSERLSDGIFPCILNRVYETPDAFGGNVGCCAMFGGDQRTKCA
                     GKDLMPSEGSDGIGIG"
     gene            complement(199..756)
                     /locus_tag="ACFB2Q_001184"
     CDS             complement(199..756)
                     /locus_tag="ACFB2Q_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC0918215.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQILQAATQVSDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFVPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCEDKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(831..2465)
                     /locus_tag="ACFB2Q_001185"
     CDS             complement(831..2465)
                     /locus_tag="ACFB2Q_001185"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC0918216.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCARATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVYYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2528..2869)
                     /locus_tag="ACFB2Q_001186"
     CDS             complement(2528..2869)
                     /locus_tag="ACFB2Q_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918217.1"
                     /translation="MIFKCRPNVFQTASAVSAFSGGGWGCGVKLRCRRLSVLFNPGVM
                     LFVPDCPVGGASGFFQYEMLCPLFRQGRLQTGSNFAYDVFMSAGRLNGGRKPPRRLLV
                     FLPCFAPLPYN"
     gene            complement(2866..3972)
                     /gene="serC"
                     /locus_tag="ACFB2Q_001187"
     CDS             complement(2866..3972)
                     /gene="serC"
                     /locus_tag="ACFB2Q_001187"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC0918218.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFSVMEMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYQIVPKLSDGLPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4338..4769
                     /gene="rimP"
                     /locus_tag="ACFB2Q_001188"
     CDS             4338..4769
                     /gene="rimP"
                     /locus_tag="ACFB2Q_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC0918219.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELAAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4797..6299
                     /gene="nusA"
                     /locus_tag="ACFB2Q_001189"
     CDS             4797..6299
                     /gene="nusA"
                     /locus_tag="ACFB2Q_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC0918220.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6311..9142
                     /gene="infB"
                     /locus_tag="ACFB2Q_001190"
     CDS             6311..9142
                     /gene="infB"
                     /locus_tag="ACFB2Q_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC0918221.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRTHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9302..10642)
                     /locus_tag="ACFB2Q_001191"
     CDS             complement(9302..10642)
                     /locus_tag="ACFB2Q_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC0918222.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GGARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(10716..12056)
                     /locus_tag="ACFB2Q_001192"
     CDS             complement(10716..12056)
                     /locus_tag="ACFB2Q_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC0918223.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRSEMIDRDRMLRDTLERVRAGSFWLWVVVASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12199..12825
                     /locus_tag="ACFB2Q_001193"
     CDS             12199..12825
                     /locus_tag="ACFB2Q_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC0918224.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12890..14308)
                     /locus_tag="ACFB2Q_001194"
     CDS             complement(12890..14308)
                     /locus_tag="ACFB2Q_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC0918225.1"
                     /translation="MENILSVLVGTVNRFLWGYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKQ"
     gene            14635..15363
                     /locus_tag="ACFB2Q_001195"
     CDS             14635..15363
                     /locus_tag="ACFB2Q_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC0918226.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIGLRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRTQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15422..15910)
                     /locus_tag="ACFB2Q_001196"
     CDS             complement(15422..15910)
                     /locus_tag="ACFB2Q_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC0918227.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
BASE COUNT         4256 a         4644 c         4144 g         3197 t
ORIGIN      
        1 ccccgattcc gatgccgtct gaaccttcag acggcatgag gtctttgcct gcgcacttgg
       61 tgcgctggtc gcctccgaac atggcgcaac acccgacatt tccgccgaac gcatcgggcg
      121 tttcataaac ccggtttaaa atgcatggaa aaatgccgtc tgaaagcctt tcagacggca
      181 ttttgtttga gattccgttt accaatggct gacaaacgct tccaaatcgg tattcttggg
      241 cttatgcact tcctctgtcg gcgtgccgac catcatcagc ccgatgattt tatccttatc
      301 ctcgcaaccg aaagcctccc gcaacaatgg gctgttgacc cacatccccg taatccagac
      361 attgtcgaat ccctgagccg ttgccgccag ttgcagcgca tacgccgcac aacccgccgt
      421 cagcatctgc tcccattccg gtttcggctt aggcacatcg cggttcggca caaacgttac
      481 cccgatgacc atcggcgcca tattgcccac tttttccgcc tttttcatcg catcgtcgcc
      541 gaaattcagt tcggcaaccg tttgcttcaa cacatcgcga aaacgttgca atcctacctc
      601 gccttgaatc acggtaaaac ggaaggggcg catgttgccg tgatcggaaa cttgggttgc
      661 cgcctgcaat atttgttcca actccgccgc atcgggggcg gggtgcttca gctttttgga
      721 agatcggcgg ttcgtcaata attttaaagc atccatatcg ttatactccg gtcatcggtc
      781 gggtgtttcg gacacatatg ccgtgaaggc ttcagacggc atatccggca tcagcgcgga
      841 cggcggcagg ctgccaatat atccatttcc ttccgatagg tttggctgtt ggaaatgtcc
      901 atcagcccca ataccgtgct gaaatagtgg tcgtgcgaat attcgttttc cgccgctttt
      961 tgtttgaggc attggaaatc tatgcccccg tgttggcgga aggctttgga aaaccacata
     1021 accatcggga tatgcgtctg tccggaaggc gcgatggcgt aaggcgcggc gtgcaggtac
     1081 atcccgtttt cgcccaaact ttcgccgtgg tcggaaacat aatacaccgc gctttccaaa
     1141 tcgtcgcggt tttcaagttt gcggataacc ttgtcgataa actgatccac atacaaaacc
     1201 gtattgtcgt aagtgttgac caacgtggcg cgggcgcatt tgtcgatttc gttggtatcg
     1261 catgtcggcg tgaatttgcg ctcggcttcg gtgtagcgtt cgtaatacgt cggcccgtgg
     1321 ctgccgatgg tatgcaggat taaaaccgcg tctttatcgt ttttgttgag ggcttcgtcg
     1381 aacttggtca acaggatatt gtcgaggcac tcgccgttgc ggcagtattc gggcaggttg
     1441 agcgaagtaa cgtcggtatt cggcactttg ccgcacacgc ccttgcagcc ggaatcgttt
     1501 tccaaccaag taacttccac gccggcgcgc tgcacgatgt ccagcaggtt gtcttggtgt
     1561 tcggctttga tttcgtcata atccgtgcgg tcgaaggttg agaacataca cggcagggag
     1621 tgcgcggtcg atgtgccgca gcttctgacc tgcgggaaat tgacaatttc atcgccgcgc
     1681 gcggcaagca gcggcgtagt ttggcggctg taaccgttca aaccccagtt ggcggcacgc
     1741 gtagtctcgc ccacgaccag caccacgaaa cggcgcaggc tgccggccgg ccggttttgc
     1801 acgaccgcca tatccaattg cgtataagga atattggaac gcttccaatc tttgtatttc
     1861 gacacgcccg cgccgatgaa attagacggc acaatcagat gggttactga tttattgttg
     1921 cggaaaaacg aggcgtaatc ctgatattgc aacatcgcga tgcccaacgc gcacaaaaag
     1981 gaaacggcgg caagcacaag gcgcgtcaaa agctccttat accaaacgcg gtatttgacc
     2041 ttgacggcga tatatgccag cgcgggcaat acgcccaaac atacaatcca cagcacatag
     2101 cccggcgtaa tcaggcgcgc gctttccgcc gccgtggttt gcaagacatt attcaacatc
     2161 gacttgttga aatagatatt gaaaaatatt tcttggtaag acaccgccgc actgataacc
     2221 aatatcaatg gaatcaacac tttatgcaca aaaggcaggg caatgacgtg aaaaacgaaa
     2281 ttacttaaaa aaaacagcac caccggcatc gtatagagga agatgtccgc cccggtgtcg
     2341 ttaaaagggc gaagttcgac aactttggca aaaaaggcgt aattcaatac cagcgaagaa
     2401 tacagggaaa gaaaggcaat cagcgcggaa gagccgagct tcggcctcag gttcggtttt
     2461 atcatttgga atgtgtcgga taagggttgg aaaaggcatc cggaatccgg attattgaaa
     2521 aagattctta attataaggc aacggagcaa agcagggcaa gaaaacgagt aggcggcgcg
     2581 ggggtttccg cccgccattc aaacgtccgg cagacataaa aacatcgtaa gcaaaattcg
     2641 aaccggtctg caaccgcccc tgccggaaaa gcgggcaaag catttcatat tggaaaaacc
     2701 cggacgcgcc gccgacggga cagtccggca caaacagcat cacgcccgga ttgaaaagga
     2761 cggatagccg ccggcagcgc agttttaccc cgcatcccca accgccgccg gagaacgccg
     2821 aaacagccga tgccgtctga aacacattcg ggcggcattt gaaaatcaac cgtaacgccg
     2881 ttggaaatcg cgcataaaat ctgccaaagc ccgcacgcct tcaagcggca tcgcattata
     2941 aatgctggcg cgcatcccgc cgacagactt atagcccttc aacaggcaca agccctgcaa
     3001 ctcggcttcc aacacaaaac ggcggtcaag ctcctcatcc cccgtttgga acacgacatt
     3061 cattttagaa cgcgcattcg gacggatacg gttgatatag aaaccgccgc tgccatctat
     3121 cgtctcatac aaggtttgcg ccttcagccg attgaccgct tcaatttttt tcacaccgcc
     3181 ctgcgcctgc agccagcgga acaccagccc cgacatataa atcgcgtaag ttgacggcgt
     3241 gttgtacata ccgtcgcggt tcaaatgaga acggtagttg aacacatcgg gaatatcgtt
     3301 cggacaacgc tcgagcaaat cctcacgcac aatcaccacc gtaactcctg ccggcccgat
     3361 gtttttctgt gcgcctgcgt aaatcagtcc gtagtcggca acatcaaact cgcgcgacag
     3421 aatctcgctg gacatatcgc acaccaacgg cggcaggccg tctgaaagct tggggacgat
     3481 ttggtattgc aggccgttga cagtttcatt gacggcaaaa tggacaaacg ccgaatcggg
     3541 tgcaacatcc cacgtttcca caggcggcag gtcaagatag tcgaactgct cgcccccgtg
     3601 cgccgccaaa cggatttccg tatcggtcaa acggctcatc tgttcatagg cgatacggct
     3661 ccagttgccc gttaccaccg cgtcggcagt gcggaaaccg tgtgccagat tcatggctgc
     3721 catattaaat tgggttgttg ctccgccctg aagaaacaat atcttatagt tgtcaggcac
     3781 tttcaaaagc tgcctcaaat cctgttccgc atgatgcagg atgctcaaaa acatttccga
     3841 acggtggctc atttccatca cggaaaaacc cgtaccgttg tagtccgaca tttcctgctg
     3901 cgccgtcctc aacacagctt caggcaacac ggcagggccg gcggaaaaat tgtaaatcgg
     3961 ataaagagac atgatgcagc cctggttctg aacaataacc cgcccgattt taggcttgca
     4021 gcaggctttg tgcaaggatg ggaaatacct gtcctccgcc cgattccatg ccgcccgaac
     4081 acggaaaata atatcaatat attgatttac aaacataaaa atcatgctag cgacaaatag
     4141 atacatttgt tttgtcaaca atattcacga tttcccatta caaacctccc gtacacccgc
     4201 ttttttccgt cccaaaaaca caaaataaat caacactttc atttctccgc aaaagcggtt
     4261 ataatcacgc cgatttttca acttttgacg aaaaatggcc acggggccat ttttttatta
     4321 tgtacatagg gagcagcatg gatattcaaa ccatcctcga aaaaaccctg cccggcctgg
     4381 gatacgaact ggtcgatttc gaactggccg cgcaaggaac attgcgcgtg ttcatcgaca
     4441 aagaaggcgg cattaccgtc gaagactgcg ccaccgtcag caaccacttg agccgcgtct
     4501 tcatggttga agacatcggc tacaaaaacc tggaaatttc cagccccgga ctcgaccgcc
     4561 ccttgaaaaa agccgccgac ttcgtgcgct ttgccggtca gaatgccaaa atcaaaaccc
     4621 gcctgccgat aggcggtcag aaaaacttta tcggtaaaat cgaaggctgc gaaaacgaca
     4681 ctgttaccgt ttccttcgac ggcaaaaccg tgcaaatcga attgggcaac atcgacaaag
     4741 cccgcctgcg ccccgaattc aaattctaaa acacagcaat attggagatg ttcaaaatga
     4801 gtcgtgaaat gttacagctg gcagaagcac tggcaagcga aaaaaacgtt gatgcggaag
     4861 tcgtcttcca agcactggaa ttcgcactct ctaccgccgc caagaaaaag gcagaccgcg
     4921 aacacatgga cgtgcgcgtc caaatcaacc gcgataccgg cgaataccaa accttccgcc
     4981 gctggctgat tgtggccgat gaagactata cctatcccga tgtcgaaaaa accatcgagg
     5041 aaatccaaga ggaaatcccc gatacgacca tccaaatcgg cgaatactac gaagagcagc
     5101 tgcccaacga aggcttcggc cgccaagcgg cgcaaactgc caaacaaatc atcctgcaac
     5161 gcatccgcga tgccgagcgc gagcagaatc tgaacgagtt cctcgccgtc aaagaagaca
     5221 tcgtgtccgg cacggtcaaa cgcgtcgaac gccacggcat catcgtcgaa gtcgttgccg
     5281 gcaaactgga cgcgctgatt ccgcgcgacc aaatgattcc gcgcgaaaac ttccgcagcg
     5341 gcgaccgcat ccgcgccctc ttcctgcgcg tcgaagaaat cggcaacacc ggccgcaaac
     5401 aagtcatcct gagccgtact tccggcgatt tcctcgtcaa actgtacgcc aatgaagtac
     5461 ctgaaattgc aggcggcatg cttgaaatcc gcgctgtcgc ccgcgacccg ggccaacgtg
     5521 ccaaagtcgc cgtcaaagcc aacgaccagc gcatcgatcc gcaaggcacc tgtatcggcg
     5581 ttcgcggttc gcgtgtcaat gccgtcagca atgaattgtc cggcgagcgc atcgacgttg
     5641 tcctttggtc gcccgagcct gcccaattcg tcatgagcgc gctctcaccc gccgaagtca
     5701 gccgcatcgt catcgacgaa gacaaacacg ccgtcgatgt catcgttgcc gaggaccggc
     5761 tcgcgctcgc catcggacgc ggcggtcaaa acgtgcgcct tgcttccgac ctgaccggct
     5821 ggcagctcaa catcatgact tccgccgaag cagacgaacg caatgcggca gaagatgccg
     5881 ccatccgccg cctgtttatg aatcacttga atgtagatga agaaaccgcc gacgtactgg
     5941 ttcaagaagg ttttgcaacc ttggaagaag tcgcctatgt tcctgccgcc gaactgcttg
     6001 ccattgaagg atttgacgaa gaaatcgtcg atatgctccg caaccgtgcc cgcgatgcca
     6061 tcctgaccat ggcgattgct gccgaagaaa aactgggcga agtgtccgac gatatgcgta
     6121 acctcgaagg cgtagatgcc gatatgctcc tcagccttgc cgaagcaggc attaccaccc
     6181 gcgacgactt ggcagagctt gccgtggacg aactgattga aatcaccggt gtaaacgaag
     6241 aaactgcaaa agccgtcatc ctgaccgcac gcgaacactg gtttaccgaa gacaaataaa
     6301 gggggtacag atgagtaaca caaccgtaga acaatttgcc gccgagctga aacgccccgt
     6361 cgaagacctg ttgaaacagt tgaaagaagc cggcgtcagc aaaaacagcg gcagcgattc
     6421 cctgacgctg gacgacaaac agcttctgaa cgcctacctg accaagaaaa acggcagcaa
     6481 cggcggcacc atcagcatcc gccgcaccaa aaccgaagtc agcaccgttg acggcgtaaa
     6541 agtcgaaaca cgcaaacgcg gacgcactgt caacattcct tctgccgaag aattggcagc
     6601 acaagtaaaa gccgcccaaa cccaagccgc acctgttcag ccggagcaga cggcagaaga
     6661 cgcggtaaaa gcccgagccg aagctgccgc acgcgcagaa gcccgtgcca aggcagaagc
     6721 ggaagcggca aaactgaaag cggcaaaagc aggcaacaaa gccaaacctg ccgcgcagaa
     6781 acctaccgaa gcaaaagccg aaaccgcacc cgttgcggcg gaaaccaaac ccgccgagcc
     6841 caaagaaaaa gccgtcaagc cgaaacacga gcgaaacggc aaaggcaaag atgccaaaaa
     6901 accggcgaaa cctgccgcac ctgccgtgcc gcaacccgtg gtcagcgcgg aagaacaggc
     6961 gcaacgcgac gaagaggcgc gccgcgccgc cgcattgcgc acccaccagg aagccctgtt
     7021 gaaagagaaa caggaacgcc aggcacgtcg cgaagccatg aaacaacagg cagaacaaca
     7081 ggcaaaagcc gcacaggaag ccaaaaccgg cagacagcgt cccgccaaac ctgccgagaa
     7141 accgcaggca gccgcaccgg ccgtcgaaaa caaacctgtc aatccggcaa aagcgaaaaa
     7201 agaagaccgc cgcaaccgcg atgacgaagg tcaaggccgc aacgccaaag gcaaaggtgc
     7261 aaaaggcggg cgtgaccgca acaatgcccg caacggcggc gacgagcgcg tacgcggcgg
     7321 caagaaaggc aaaaaactca aactcgagcc gaaccaacac gccttccaag caccgaccga
     7381 acccgtcgta cacgaggttt tggttcccga aaccattacc gttgccgatt tggcgcacaa
     7441 aatggcggtc aaaggcgtgg aagtggtcaa agccctgatg aagatgggca tgatggttac
     7501 catcaaccaa tccatcgacc aagacaccgc cctgattgtc gtcgaagaac tcggacacat
     7561 cggcaaaccc gccgccgccg acgatcccga agccttcttg ggcgagggcg cggaagcggt
     7621 ggaagccgaa gcattgccgc gtccgcccgt cgttaccgtc atgggccacg tcgaccacgg
     7681 caaaacctcg ctgctggact acatccgccg cgccaaagtg gtacagggcg aagcgggcgg
     7741 cattacgcag cacatcggcg cgtaccacgt caaaaccccg cgcggcgtga ttaccttctt
     7801 ggacaccccg ggccacgaag cctttaccgc tatgcgcgcg cgcggcgcga aagcaaccga
     7861 tatcgtgatt cttgtggtcg ccgccgacga cggcgtgatg ccgcaaacca tcgaggcaat
     7921 tgcccacgct aaagcggcag gcgttccgat tgtggttgcc gtcaataaaa tcgataaaga
     7981 cactgccaac cccgaacgca tccgtcagga actgacccaa cacgaagtca tccccgacga
     8041 ttggggcggc acggttcaat tcatcgacgt ttccgcgaaa aaaggaacga acatcgacgc
     8101 gctgctcgaa gccgtattgc tcgaagccga agtattggaa ctgaccgcac ctgtcgatgc
     8161 gcccgccaaa ggcatcatcg tcgaggcgcg tttggacaaa ggacgcggcg cggttgccac
     8221 attgctggtt caaaacggta cgctgaaaaa aggcgacatg ctgctggccg gtacggcatt
     8281 cggcaaaatc cgcgcgatgg tcgatgaaaa cggcaaatcc attaccgaag ccggcccgtc
     8341 catccccgtc gaaatcctcg gcttgtccga cgtaccgaat gcgggtgaag acgcgatggt
     8401 attggcggac gagaaaaaag cgcgcgaaat cgccctcttc cgccaaggca aataccgcga
     8461 cgtgcgcctt gccaaacagc aggcggcgaa gctggaaaat atgttcaaca atatgggcga
     8521 aacccaggcc caatctttgt cggtcatcat caaggcagac gttcagggct cttacgaggc
     8581 tttggcgggc agcctgaaaa aactgtccgc cgacgaggtg aaagtgaacg tgttgcacag
     8641 cggcgtgggc ggcattaccg aatcggatgt caaccttgcc atcgcttcgg gcgcgttcat
     8701 tatcggcttt aacgtgcgtg cagatgcctc ttcgcgcaaa cttgccgaaa atgaaaacgt
     8761 ggaaatccgc tactacaaca tcatctacga tgccatcgac gacgtgaagg cggcgatgag
     8821 cggcatgctt tctcccgaaa agaaagaaca ggttaccggt acggtcgaaa tccgtcaggt
     8881 catctccgtt tccaaggtcg gcaacattgc aggctgtatg gtcaccgacg gcgtggtcaa
     8941 acgcgattcc catatccgcc tcatccgcaa caacgtggtc atccacacgg gcgaactggc
     9001 ttcgttgaaa cgctataaag acgacgtaaa agaagtccgc atgggcttcg agtgcggtct
     9061 gatgctcaaa ggctacaacg aaatcatgga aggcgaccaa ctggaatgct tcgacatcgt
     9121 cgaagttgcc cgcaccctgt aattcctttg caaataaaat gccgtctgaa gcgttcagac
     9181 ggcaacgaaa cgggttctgt gtcatacaga acccgttttt tgtcgcaaat cggcttcagc
     9241 gaccctcctg ccttatcccg atttgaatct gacttgccat acaaacaggc ttcagacggc
     9301 attatttgcc cgccaaacgt atcccgatct tctccgcata ttccctgcgt tcggcgcggc
     9361 tggtttccgg gcggtgcgta ttgagcgacg accatttcca atgactgcgg gctttgttga
     9421 gttcgggcgg gagtttggcg gcatcccacg gggctttgcg gctgtgcagc tcgatatccg
     9481 actgtgccgc gtgtccgcgc gtttgcagga cgtggagcag atcgagggcg cgggcggcga
     9541 gcagggtcag ggtttcaggg tcggtgtgca gggtttgacg gccggcgagt ttgtcggaaa
     9601 tggtgcgggt attgggcagg atgccgccca aaaagccgcc gattgccgta cccaatccga
     9661 gcgagccgcc gagtgtggcg atgtccagcc ccaagccgat gagcgcgccg gttgccgcgc
     9721 ccgtgccggt gcggatgccg tattgtttga gcaattcgct gtcgaacggg tcttggcgga
     9781 aggcttgcgg catccagtcg ccgccgtcga tttcgctgtg gtagaaacgg taaagggcaa
     9841 acagccgctg ctgcatctgc cgttcgagtt ggcgtatttc cgcctgcatg gtttgcagca
     9901 cggtggcggt atcctcgttt tcgtccactt cctgcctgaa ggcggcggca tcgagcagga
     9961 aatcggcgat ttcgcggcgc gctccgccgt ccagccgctg ccattcgcgc cggcgcatgg
    10021 ctgtcaggcg gtcaagtgtg ctgcgttcgg gcaacatggt ggcgaggttt tcccacaggc
    10081 gcaattcgcc ttcaaaatca aaggcgacgg tgtcgaaccc tgcgaaaacg tgcaggtttc
    10141 tcctcgccag catggttgtc cacgattcgg gaagctgtcc gccggtaaag ttgaacacgg
    10201 gcataaccgg tttggcacac catgaaagga tggtcaattc atcccgatat ttgtcgagga
    10261 cgggttcgcg cgcgtcgatg acgtacattg ccatatcgct ttgcaaaacc tgccgcagca
    10321 ctttggcttc ttggttgaaa tcgtgatgcg cgccgtggct gccgagaaac tgttgcagcc
    10381 gctcgatgcc gtctgaacga ttgtccgtat ggttttccag ccattccaaa acaccgcccg
    10441 cgtcttcgag tccgggcgtg tcatacagga aaaccaacat gtccgcgccg tcgctgatgg
    10501 cggcttcttc gacatggcgt gtggtggacg gtgcgtttct gacttcgccg aagccgctgt
    10561 cgcgcaacag ggtgcgcagg agcgaggttt tgccggtgtt ggtgtgtccc acgacggcga
    10621 gggaaagggg ttgtttgttc atgatgtttt tgaagaatgg attttcagac ggtctttttt
    10681 cagaatggcg gcttaacaga acatttcaag tgagtttatt ggtctttcaa acggccttcc
    10741 tgcgccaccc tgtcaggctc aagccacgcc gcgccgcatt cggtcagcgc gttacgccaa
    10801 tgttccagct tttccgaaag gtcgtctgaa agcccctgtt ccgccaaaag ctgcaccacc
    10861 gcgccgccct gcgccgcttc cgaaagccgc acaatctgcc gcagcacgcc ccggtccggc
    10921 acagtttggg cgcgtacgcc gataagcagt tgcgccggtt tctgcttcag ctctgtctcc
    10981 agcgcggcaa cctgttcccg attggcggca acgcccttat ccagccattc ctgcgccagc
    11041 ctgccctcga accattggcc gtcctgccac tcggtctcca gcatgagcgc ccatttcggc
    11101 gcatcgttca agacgatttt cggcgaaacg gcggacacgg tttcccgacg cgtatccgca
    11161 tcggtgattt tgttctgcca gcggcggatg accgcctgat aataggtttt ttccaaatcc
    11221 aatccgtttt cgcttgtttt caaaaggatt ttacacacta cccaagccaa gaggcgcggc
    11281 aggatgccgt agcagacgat actgccgacc agcagccccg accaagcccg cgcatcggca
    11341 atattgccgt tcagacgacc ttcgatgacc gcccgcgcat cggggacagg gaaaccgagt
    11401 ttcgacggca gccatgccaa catttccacc gcgcgtaccg aagcggcatt gctcaacagc
    11461 gtgctttccc agttgaacgt atattgccgc accaaaagca gcagcaatac cgacaccagc
    11521 attccgagca gcgtgcagag ccacaagctg tgcgccgttg cgcctatttt ccatcgtacc
    11581 gaaggttgcc gccactggtc cgcatacagc cgcaacaccg cctgatttac agggcctttg
    11641 ccccgaaacc acgtcgccgg actgctgaaa aaccgcccca ctttcacgcg caggaacaac
    11701 gttgccaacc atactgccag catcagcgta ttcatgccca acactcccgc caaaactaaa
    11761 aagaaattca gcccctgatt gtccatcaga agataagtgc ctgaaaatcc ggcggtaaac
    11821 atcatcgatg ccaccaccac ccataaccag aacgaccccg cacgcacacg ttccaacgtg
    11881 tcccgcagca tacggtccct gtcgatcatc tccgaccgac ggatgatttt ttcctccgta
    11941 ctgccgtcca cgcggcgcaa agcctccgtc gcctgcacgg gatcgccgct gaaaataaaa
    12001 ccgccttcat ccaaaatacg gaccagctca accagttttc gggatggatt caacataaaa
    12061 tgccgtctga aaataaaaaa caaattttaa cacacgcatt ttcaagaata ttcacagtgt
    12121 aggcaaagag taaatcccac acagaggcaa aagtatcggc gtaaactgac tgcctctact
    12181 ttcccgaaag attgtgcgat gtatgcaggc gaacgcttca atacttacag ccatttgagc
    12241 ggtttgattc tggcggcggc aggtttgatg ctgatgctgc tgaaaaccat aggacacggg
    12301 gacggctacc gtatcttcag cgtatcggtt tacggcatca gccttcttct gctctatttg
    12361 agttcctcgc tgtaccacgg aattgcagcc ggaaaactga aaagcatttt gaaaaaaacc
    12421 gaccactgca tgatttatgt gctgattgcc ggaagctaca caccgtttgc actggtttct
    12481 ttgagaaacg ggccgggctg gacggtattt tcactgtcct ggctgctggc ggctgcagga
    12541 atcgcacaag aactcaccat cggacggaaa agcgaaaaac gtctgctgtc tattgcgatt
    12601 tatatcgtaa tgggctggat ggtcttggcg gtaatgaaat ccctgacagc ctcactcccg
    12661 ccggcaggac tggcttggct ggcggcaggc ggtatgctgt acagcgtcgg catttactgg
    12721 tttgtaaacg atgaaaaaat ccgacacggg cacggaatct ggcatctgtt cgtattgggc
    12781 ggcagcataa cccaatttgt cagcgtgtac ggttatgtaa tctgaatgcc gtctgaaaca
    12841 caaaaacctc ccaaacctga aggcagggag gttttctctt tgccgggcat tattgtttat
    12901 cgtggaactc gtggaattca tactgatagg acaaatcccg acccgctttt ttctgtgcca
    12961 aataatcatc ataaatggcg cggatttcct tacgcagcaa gaacagggca atcaggttgg
    13021 ggataaccat aaagccgttg aacatatcgg acagattcca aaccaaatcg actttgccga
    13081 gcgtacccaa aacgatagca agcagaacca atgcgcgata gatgcccaag tgtcttcccc
    13141 tgaaaagaaa acggatgttg gactcgccga aataatacca gccgataatg gtcgtaaagg
    13201 cgaagaaggt cagacacacg gcaagcaatt gcgaaccgaa gcccggaaat gccttgttaa
    13261 aggcaaattg agtaaccgcc gcgccctgtt cgcccgaaag gttggcatcg gtcagcagga
    13321 taatcaatgc cgtagccgta cataccaaaa tcgtatcgat aaatacaccg acaaatgccg
    13381 tcataccttg ctgaacagga tgcttcacat ccgcagtcgc gtgggcgtgc ggagtcgaac
    13441 ccatacctgc ttcgttggaa aacagaccgc gcgccacgcc gaaacgtatc gcttcacgca
    13501 taccgatacc cgcagcaccg cccaaaacgg cctcgggatt gaaggcggcg gtaaagatgt
    13561 ggttgaacat cggcacaata tggtcggaaa attcaaacag gataacgacg gcgcacaaaa
    13621 tataaacaac cgccataaac ggcacgacaa accgggcaat attggcaata cggttcacgc
    13681 cgccaatcac aaccatgccc gcaaggacgg caaacacaat accgactgcc aaagaaggca
    13741 catcaaatgc aatggtaacg gcagaagcga tggagtttgc ctgagtcgca ttgccgataa
    13801 agcccaacgc gacaatcagc gcgatggaga aaaagcccga caggaaacgc gccgcgcccc
    13861 tgccgatttt cggagtcaga ccgtgcgtaa tataaaacgc cggcccgccg atgtatttgc
    13921 cgtggctgac gacgcggtat ttctgcgcca gcagtgcttc cgcaaaaatc gtggacatcc
    13981 ccaaaacggc agaaagccac atccaaaaaa tcgcacccgg cccgcctgcg gtaatggcgg
    14041 tcgccacacc ggcgacgttg cccgtaccga tttgcgcgga tatagcaacc gccaacgcct
    14101 gaaactgcga taaggactta tcgtctttat cacctttggc aaacaagccg ccgaatacgg
    14161 atttgaatcc cgcgcccagc ttggtaatct gcggcgcacc aagatacagc gtaaaaaaca
    14221 ggccgatacc caaaagcgca taaatcagca ggtagcccca aagaaaccga ttgactgtac
    14281 ccaccagaac agacaatata ttttccataa aataaacctt atcttacgat taaaatgact
    14341 gttttccaaa agacattcca ataaggaaac acggcgagca gaccgtattt gccgcaacag
    14401 atgccttaaa ttgtcaacaa tccgagagaa gctgtgccag cataccgtaa aatatcgtta
    14461 attaaaatat ttctttattt ttaagcggaa agcggaggaa atcgttttca gacggcatcg
    14521 acaacggcac ggcataaaac aggatatttt gggtacttgc aacttatgtt aaaatgccga
    14581 ccgtaaaaaa tctgacaaaa acagattaat tatttgaaat aagaaaggaa atttatggca
    14641 ggccatagca agtgggcgaa tatccagcat aaaaaagccc gtcaggatgc caaacgcggc
    14701 aaaatcttca cccgtttaat caaagaaatc accgttgcgg cgcgtatggg cggcggcgat
    14761 cccggcgcaa atccgcgcct gcgtctggct ttggaaaaag cagccgaaaa caatatgccc
    14821 aaagacaatg tgcaacgcgc catcgacaaa ggtacgggta acttggaagg cgtggaatac
    14881 atcgggttgc gctacgaagg ctacggcatc ggcggcgcag ctttgatggt ggactgcctg
    14941 accgacaaca aaacccgcac cgttgcggac gtacgccacg catttaccaa aaacggcggc
    15001 aacttgggta ccgacggctg cgtggcgttc aacttcgtgc atcagggcta tttggtattc
    15061 gaacccggcg ttgacgaaga cgagctgatg gaagcggctt tggaagccgg tgcggaagac
    15121 gtggttacca acgacgacgg ttccatcgaa gtcattaccg cgccaaatga ttgggcgggc
    15181 gtaaaatccg ctttggaggc ggcaggttac aaatccgttg acggcgacgt tacgatgcgc
    15241 acccaaaacg aaaccgaact ctccggcgac gatgccgtca aaatgcaaaa actgattgac
    15301 gcgctggaag acttggacga cgtgcaagac gtttacactt ccgccgtatt gaatctggac
    15361 tgatacgcag cacagcagac atacaacaaa atgccgtctg aacctttcag acggcattga
    15421 tttatttagc ccttgcccac gcccaccaaa ccgtcaggac aaccgccaaa aaatacgccg
    15481 cgctccaaac aggcaaagca acccctaaca gataatccgg ttcggcacaa tttccgaacc
    15541 cgcgcacaac aggctcgaac caatcaaaca aaggccagcc cttcaatcga aacgtccacg
    15601 gcgcgccgca cgaaggagcc gtacccggcg gcagcgactg caaccacaac tgatatgccg
    15661 caacagaaat acccgtaacg gcgggaatac tgataaacac tgcgcctgac agtccgccca
    15721 ccctgccctt gggcttacac gccagcacaa ctgccgcaca caatgcggtt gccaaaacac
    15781 ataaccgctg actgatgcac aaaacgcaag gctccatacc caaaacatac tgtgccgcca
    15841 aagaaccggc aaatgcacag accgaaacgg caaacagcag ccaaacggct tttctaaata
    15901 acggggtcat tttctcaaca caccaatcaa aataccgata tgccgatttt gctggatata
    15961 tcccgagatg gcaagggacg aatcgacacc ggcacaggtg cggacgattt cggttttccc
    16021 gatatgcaca aaacctgaca aaggacaaaa cggcgatttg tccgcacaat gcccgaaaaa
    16081 atacatccgg aggatttgaa tttgaatttc ggcaaattta gcgaatcaaa agcaacagcc
    16141 gataaaaaaa tcaaaagctt atttcaatgt ttcaacacac aggacgacac ataaagcacc
    16201 gccctatgtg ccgtcctgat ttggaagggg ttacacccct c
//
LOCUS       DAXDSD010000049        16196 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXDSD010000049 DAXDSD010000000
VERSION     DAXDSD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16196
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.049"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..147
                     /locus_tag="ACFB2Q_001197"
     CDS             <1..147
                     /locus_tag="ACFB2Q_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFC0918228.1"
                     /translation="SDIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAI
                     RGNI"
     gene            188..718
                     /locus_tag="ACFB2Q_001198"
     CDS             188..718
                     /locus_tag="ACFB2Q_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC0918229.1"
                     /translation="MNAIRTFQNRMPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYIGSPVRQVRLLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(886..2481)
                     /locus_tag="ACFB2Q_001199"
     CDS             complement(886..2481)
                     /locus_tag="ACFB2Q_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC0918230.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFGIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(2588..2983)
                     /gene="hisI"
                     /locus_tag="ACFB2Q_001200"
     CDS             complement(2588..2983)
                     /gene="hisI"
                     /locus_tag="ACFB2Q_001200"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC0918231.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(3014..3781)
                     /gene="hisF"
                     /locus_tag="ACFB2Q_001201"
     CDS             complement(3014..3781)
                     /gene="hisF"
                     /locus_tag="ACFB2Q_001201"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC0918232.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRTMREAGIEVRL"
     gene            complement(3794..4531)
                     /gene="hisA"
                     /locus_tag="ACFB2Q_001202"
     CDS             complement(3794..4531)
                     /gene="hisA"
                     /locus_tag="ACFB2Q_001202"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC0918233.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDLVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQSVRIPVIASGGLTGLDDIPALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(4564..5202)
                     /gene="hisH"
                     /locus_tag="ACFB2Q_001203"
     CDS             complement(4564..5202)
                     /gene="hisH"
                     /locus_tag="ACFB2Q_001203"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC0918234.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5332..6834)
                     /gene="pta"
                     /locus_tag="ACFB2Q_001204"
     CDS             complement(5332..6834)
                     /gene="pta"
                     /locus_tag="ACFB2Q_001204"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC0918235.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7019..8077)
                     /gene="fbpC"
                     /locus_tag="ACFB2Q_001205"
     CDS             complement(7019..8077)
                     /gene="fbpC"
                     /locus_tag="ACFB2Q_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC0918236.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8098..9615)
                     /gene="fbpB"
                     /locus_tag="ACFB2Q_001206"
     CDS             complement(8098..9615)
                     /gene="fbpB"
                     /locus_tag="ACFB2Q_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC0918237.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(9683..10678)
                     /gene="fbpA"
                     /locus_tag="ACFB2Q_001207"
     CDS             complement(9683..10678)
                     /gene="fbpA"
                     /locus_tag="ACFB2Q_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC0918238.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(10930..11410)
                     /locus_tag="ACFB2Q_001208"
                     /pseudo
     CDS             complement(10930..11410)
                     /locus_tag="ACFB2Q_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(11468..12844)
                     /gene="argH"
                     /locus_tag="ACFB2Q_001209"
     CDS             complement(11468..12844)
                     /gene="argH"
                     /locus_tag="ACFB2Q_001209"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC0918239.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHLLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(12863..13732)
                     /gene="galU"
                     /locus_tag="ACFB2Q_001210"
     CDS             complement(12863..13732)
                     /gene="galU"
                     /locus_tag="ACFB2Q_001210"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC0918240.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(13760..14359)
                     /gene="rdgB"
                     /locus_tag="ACFB2Q_001211"
     CDS             complement(13760..14359)
                     /gene="rdgB"
                     /locus_tag="ACFB2Q_001211"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC0918241.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSPFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(14352..14585)
                     /locus_tag="ACFB2Q_001212"
     CDS             complement(14352..14585)
                     /locus_tag="ACFB2Q_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC0918242.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(14693..15226)
                     /locus_tag="ACFB2Q_001213"
     CDS             complement(14693..15226)
                     /locus_tag="ACFB2Q_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC0918243.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15328..15786)
                     /gene="nudB"
                     /locus_tag="ACFB2Q_001214"
     CDS             complement(15328..15786)
                     /gene="nudB"
                     /locus_tag="ACFB2Q_001214"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC0918244.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            15997..16073
                     /locus_tag="ACFB2Q_001215"
     tRNA            15997..16073
                     /locus_tag="ACFB2Q_001215"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16031..16033,aa:Pro,seq:ggg)
BASE COUNT         3212 a         3965 c         5049 g         3970 t
ORIGIN      
        1 tctgatataa aaagaagggc atatcattta aggtttatgc tgttaaagtc caataagctg
       61 atatataagc tgatattccc gataatcaca ttattcgggg tcgatattac aggcaggatt
      121 ttaaaagcaa tcagggggaa tatttaataa atcctttaac aatatatacc ttaccgaagg
      181 aggaaaaatg aacgcaatca gaactttcca aaaccgcatg cccgaaatcc acgaaacctg
      241 catgatagac gaggcgtgcg tcgtcatcgg cgaagtctcg cttgccgaag atgtttccgt
      301 gtggccgtgc gccgtgttgc gcggcgatgt gaacagcatc accgtcggcg cgcgcagcaa
      361 tatacaggac ggcagcgtcc tgcacgtttc ccacaaaacc gccgccaaac ccgaagggtc
      421 gccgctggtt atcggcgaag acgttaccgt ggggcacaaa gtgatgctgc acggctgccg
      481 aatcggtaac cgcgtcttgg tcggtatggg cagcaccgtt ttggacgatg ccgttattga
      541 agatgacgtg atgatcggcg cgggcagcct cgttccgccg cgcaaacgtt tggagggcgg
      601 ctatctttat atcggttcgc ctgtgaggca ggttcgtctg ctgaccgatg aggaaaaagc
      661 ctttttgaaa tattccgccg cacattatgt gaagctgtcg aaacagtacg ggatgtgaaa
      721 tcacatcggc gttcttgcgt cagtcccaaa ttcacgcggt tgggatgccc gataacggta
      781 tccgatgcgc cttgattttg accggctgcg tttgagttgc aggcaaaaat gccgtctgaa
      841 agcctttttt tcggcttcgg acggcatttt attgccgatt tctttttaga gtttgaccga
      901 atgttcgcgc gtttcgtgga acacgatgtc cggccaacgt tcttgcgtca accccaaatt
      961 cacgcggttg ggggcgaggt aggcgaggtt gccgcctgcg tcgatggcga ggttgcctgc
     1021 gttggctttt tcaaattccg ccagtttttt cttgtcgtcg cacgataccc agcgcgccga
     1081 ccagatggat gcgctgtcga acacggcttc cacgccgtat tcgttggcga ggcgtgaggt
     1141 tacgacttca aactgcaaca cgccgaccgc acccaaaatc aaatccgcgc cgctcatcgg
     1201 tttgaatact tgaaccgcac cttcttcgcc gagttgttgc aaaccttttt gcagttgttt
     1261 gattttcagc gggtttttga tgcggacgct gcggaacagt tcgggtgcga agaatgggat
     1321 gccggtaaac gccagttgtt cgccttcgga gaagctgtcg ccgatttgga tgttgccgtg
     1381 gttcgggatg ccgatgatgt cgccggcgta ggcttcttcc gccagttcgc ggttgtgcga
     1441 catgaaggtt actacgctgg aggcggcgat ttcgcggttg atacgcaggt gtttcatctt
     1501 catgccgcgc tcgaatttac cggagcagac gcgcaagaag gcgatacggt cgcggtgttt
     1561 cgggtccata ttggcttgga ttttaaagat aaatccggaa aatttcggct cgtccggccc
     1621 gaccatgcgc atggtcgcgt cgcgcggttt cggtgcgggt gcccagtcaa tcaatgaatt
     1681 gaggatttcc tgaatgccga agttgttaat cgcagagccg aagaacactg gcgtgagttc
     1741 gccggcgaga aattcgtcga gattaaattc gttggaagcc gcctgcacca attcgatttc
     1801 gtcgcgcaac tgctggattt ccaacggaaa gcgttgttcc aattcgggat tgtttatgcc
     1861 tttgatgatg ccgaactcgt gcggcaggcg ttcgccgccc gcttcaaaga gatagatttc
     1921 gtcgttcagg atgtggtaca cgcccttgaa gtttttgccc ataccgatcg gccaggtaac
     1981 gggcgcgcag cggatttgca ggatgtcttc cacttcgtcc aagagttcca aagaatcgcg
     2041 cacttcgcgg tcgtatttgt tcatgaaggt aacaatcggc gtatcgcgca ggcggcagac
     2101 gttcaagagt ttgatggttt gcgcttccac gccttttgcc gcgtcgatga ccatcaaggc
     2161 gctgtccact gcggttaaaa cgcggtaggt gtcttcggag aagtcttggt gtcccggcgt
     2221 gtccaagagg ttgacggtgt ggtctttgta gtcgaactgc atcacgcttg atgccacgga
     2281 aatgccgcgc tgcttctcga tgtccatcca gtcggaggtg gcgaatttgc cggttttctt
     2341 acctttcacc gtgcctgcgc tttgaatcgc gcccgaaaac agcagcagtt tttcggtcag
     2401 cgtggtttta cccgcatcgg ggtgggagat gatggcaaac gtgcggcggc ggcgcacttg
     2461 gtcgaggatt tcttgggaca tggttttctt tgcaaaaagg ttcaggccgc ttttcagacg
     2521 gcccggacag tgtttgagac ggcgaaattg tacaaaaaaa tgcctgataa ttcaatgttg
     2581 gaggcggtca gtgcgtgctg ccgtaaatct ctttttcgtc tttcaggacg gcatcggcgg
     2641 tttcccacgc accgccgttc cattttttgt aaaagcagct ttcgcgcccg gtgtggcagg
     2701 cgatgccgcc gttttgggcg atgagcatca caatggtatc gccgtcgcag tcgaggcgca
     2761 gttcgcggac tttttgcgtg tgtcccgact cttcgccctt catccattgt ttttggcgcg
     2821 aacggctgta atagtgggca aagccggttt cgacggtttt ttgcagggct tcggcgttca
     2881 tccacgccac cattaaaaca cgtttggttt cggcatcttg ggcgatggcg caaaccaaac
     2941 ctttttcgtc aaatttgacg gcttcaagca ggtttttatc catatttcct ttcagacggc
     3001 atagtcgggg cggtcagagg cgcacttcga tgccggcttc gcgcatagtg cgtttggctt
     3061 cgcggatggc gatttccccg aaatggaaaa tgccggcggc aagtacggca tcggctttgc
     3121 cttcggttat gccttcaatc aggtgccgga cattgccgac cccgccggag gcgatgacgg
     3181 ggatgtcgac ggcttcggca acggcgcggg tcagcggcag gttgaaaccc tgtttcgtcc
     3241 cgtccctgtc cataccggtg agcaggattt cgcccgcgcc gcgtttttgc atttcgaccg
     3301 cccattccac cgcatccaaa ccggtcggat tgcgcccgcc gtgggtaaag atttcccagc
     3361 gtgtgttttc ggggttgacg gctttggcat cgacggcggc gacgatggct tgcgaaccga
     3421 aaaatccggc ggcttcgtta attaaatcgg gacgggtaac ggcggcggtg ttgatgctga
     3481 ctttgtccgc gccggcattg agcaggcggc ggatgtcggc aacggtgcgt acgccgccgc
     3541 cgacggtcag cgggatgaag acttgtccgg caacctcttc gatgatgtgc aggatggtgt
     3601 cgcggttgtc ggatgaggcg gtgatgtcga ggaaggtcaa ttcgtccgcg ccttcgccgt
     3661 tgtagcgttt ggcggcttcg acggggtcgc ccgcgtcgcg caaaccgatg aagttcacgc
     3721 ctttgacgac gcgcccgtct tttacgtcga gacaggggat gatgcgtttt gccagtgcca
     3781 taatcggatg cctttagtcg agggaatctg ccagttgctg cgcttgggca aaatcgatgc
     3841 tgccctcgta aatcgcgcgg ccggtaatcg cgcctgctac gccatgtttt tcggcggcac
     3901 acagggcggg gatgtcgtcc aagccggtca gtccgccgga ggcgatgacg ggaatgcgga
     3961 cggattgggc gagtttgacc gtcgcgtcga tgttcacgcc gctcatcata ccgtcgcgcc
     4021 cgatgtcggt gtagatgatg ctgttgacac cgtcgtcttc aaagcgtttt gccaaatcaa
     4081 ttacatgatg cccggttacg gttgcccagc cgtcgatggc ggccataccg tctttggcat
     4141 ccagtccgac gataatcctg ccggggaagg ctttgcacgc ttcgcgcacc aagtcggggt
     4201 ttttgactgc cgccgtgccg ataatcacgt cgttcagtcc caaatccaaa tattgtccga
     4261 tggttttcaa atcgcgcatg ccgccgccga gctgcacggg gatgtctttg gcgacggcgg
     4321 cgaggatgtc tttgatggcg ggcaggtttt gcggaacgcc ggcaaatgcg ccgttcaaat
     4381 ctaccaggtg caggcggcgc gcgccttgtt cgaaccagtg cagcgcggtg tcggcgggcg
     4441 aatcggaaaa gacggtcgcc tcttccatca gcccttgttt caagcggacg caacgtcctt
     4501 ctttcaaatc gatggcgggt atcagcagca tagtttttct ccttgtgcgg ggccgtgtcg
     4561 ggcttaccag tttaaaaagt ttttcaacat cgtcagcccg gcatcgtggc ttttttcggt
     4621 gtgaaattgc gtggcgaata cgttgtcttt gccgacgatg caggcaaacg gggacgggta
     4681 gtcgctttcg cccaatatgg tttcgggatt ttcgggggcg aaatagtagc tgtggacgaa
     4741 ataaaaacgc gtgttttggg ggatgccttg aaaaagcggg tggttttggg tttggcgcac
     4801 ggtgttccag cccatatgcg ggactttcag acggcatccc tgcgggtcgc ggaggtcgcg
     4861 ggcaaagcgt ctgactttgc cgccgaacca gcccaagccg tcggtgtttc cttcttcgct
     4921 gtggtcgaat aaaagttgcg caccgacgca gattccgaaa aacggtttgt tttttaaggc
     4981 atctttgact gcctcgtcca agccgccgcg cgtcagtgcc gccatacagt cgggcatcgc
     5041 gccctgcccg ggaaaaatga ctttgtcggc gcgggacacg cggtcggggt cgccgcttaa
     5101 aaagattttg gtattttttc cggcaagctg ccccgccgtc cggacggatt tcaatacgga
     5161 atgcaggttg cccataccgt aatcgataat ggcggtttgc atggcttcct cctctttttt
     5221 gcaatatggc tgcgatttta acaaacaaat gtgccgggct gataaaaatg ccgtctgaaa
     5281 acgggaatct gtcttcagac ggcatagggt tcaaacccgg aaagccgttt gtcagccttc
     5341 catttgtttt gcctgaacgg cagtcagggc gatggtgaac acgatgtctt ccaccagcgc
     5401 gccgcgagac aggtcgttga ccggtttgcg caggccttgc agcagcgggc cgacgctcaa
     5461 gacgttggca ctgcgttgga cggctttata ggtgcagttg ccggtgttca ggtcggggaa
     5521 gaccaaaacg gttgcctgtc ctgccaccgg gctgcccggg gctttggatt tgcctacgcc
     5581 cggcacggtt gccgcgtcat attgcagcgg gccgtcgatg gcgaggtcgg ggcgtttttc
     5641 ccgggcaagt ttggtggctt cgatgacggt atcgacatcg gggccgctgc cggagttgac
     5701 ggtggagtag gaaatcatcg ccactttcgg gtcgatgccg aaggcttttg cggaatcggc
     5761 agactggatg gcgatgtcgg caagctgttg cgcggtcggg ttcggattaa ccgcgcagtc
     5821 gccgaacacc agaacctgat ttggtagcag cataaagaat acgctggata cgaggcttgc
     5881 gcccggtgcg gttttaatca gttgcaaagc ggggcggatg gtgttggcgg tggtgtgaac
     5941 cgcaccggat accaaaccgt ccacatcatt ttgcgccatc atcatcgtac cgagtaccac
     6001 ggtgtcttgc agttgcttgc gcgcgtcttc gggtgtcagg cctttggatt tgcgcagttc
     6061 gcacatcggc tcgacgtatt gttcgaccaa tgaggcggga tcgatgattt ccaaagagtc
     6121 gggcaggctg atgccgcgtt ctttggcaac ggcttcgact tcttcgcgtt tggcaagcag
     6181 gacgcagcgg gcaatgcctt tttcgtggca gatggcggcg gcttggacgg tgcggggttc
     6241 tgcgccttca ggcaggacga tgcgtttgcc ggcttggcgg gcgaagtcga tcaggttgta
     6301 gcggaattgc gccggcgaca ggcgttttgc ttcgcggcct gccaatacgg atacgtcttt
     6361 cagcgcgtcg ctcgaaccga agaaggtcag gccggttttc tcggctgccg cttcggcaac
     6421 ggaggctgcc gcaccgtcca cgacaaaacc ttccaatacg cccggcgcgg cggcgaagaa
     6481 ctgtttggca aggttcaatt ggtgtgccag cgcgtcggca tcggtattgt cggaacggac
     6541 ggcgaagacg gctgccgcgt caagggacaa tgccagttcg acgtttttgc ctgcgagata
     6601 gattttgtcg gcatcgggcg cgatgccttc gatgacgagg ttggcggcat cgagtgcggc
     6661 aactttgccg accagtgcgt cgaaccagtc gtcgcttttg ccttgcgcga gcagggtttc
     6721 ggcggttgcg tcaacggctt ggaaaacttg tgcgtccagt gcttttgcaa aggcttgtgc
     6781 ggcggcggag gtgttcagtc cggcagatac gggtacgacg agtacttttg ccataatata
     6841 tcctttcgta tgctgcggtg tgcggcatat gtggttggaa ggggcggcat atgggcagaa
     6901 acggctgcct gcgtgccgtg cgtgccgtgt ttggcttggg gcgcgcaggt tgaatatagc
     6961 aaacaaattc tgtttccaac aagataaata tccgcaggct tgtggatgct gccgcctttc
     7021 agagggtatt tccggggaag aacagggcgg gaccgtccaa atggaggacg gcggaaatgc
     7081 cgtctgacag ggtgggggcg gaagggaggt tgagcgtgag gacggtttgt ccggccctga
     7141 ggctgatttc ggtatgccgc gctttgggcg tggttttgag aaccacggcg tgaatggagg
     7201 cggcgggtgc ggaatggggg tgaaggctga actgttccgg acggatgagc agtgtaccgc
     7261 gcgtgcctgc gggtgcgccg ctttggacgg gcaggcggcc caatctgcaa tcggcggtgc
     7321 cgtcggcgtt gagcgcggcg gggaacacga tgccttcgcc gataaacagg gcggcatcaa
     7381 ggtcggcagg ttgtcggtac aattcgtgag ggcttgcggt ttggaggatg cgcccctgtt
     7441 tcatcacggc aatccggtcg gcgtattgca gggcttcttc gcggtcgtgg ctgacaaaaa
     7501 cggcggattt tccgttggcg cgcagggcgg caatcatgtc ttcgcgaatc tggcggcgca
     7561 actgttcgtc cagcgcgctg aagggttcgt ccaacaaaat cagttcgggg tcgggggcga
     7621 gggcgcgggc gagggcgacg cgctgttgtt gtccgcccga aagttcgtgc ggatagcgtc
     7681 cggcaagttc ggaaatgccg gtcaattcca acatggcttc gatgcgctgt cgctcttgcg
     7741 ccgtcctgcc tttgccgttg ccgagaccgt aggcgatatt gcggtaaacg gtcaggtggg
     7801 ggaacagcac gccttcctgt acgaggtaac ccaaacggcg ttcgcggacg ggaaggttgg
     7861 tatttttcga gaagatggtt ttgccggaaa gcgaaatttc gccggaatcg ggttgttcga
     7921 aaccggcaag gcagcgtaaa agggtggttt tgccgcagcc ggacgcgccg atgataaaga
     7981 gaatttcgcc cgggtcgagg ctgagcgaaa tgtcgtttaa aaccggggtg ttttgaaaac
     8041 ttttggacag gtgtccgatg tgcagggcgg cggtcatagc ggtgcttcct caagctgtta
     8101 tttgaaggcg tatttcttca gcaggaatac gggtatgccg gaaaataata ccagcatcag
     8161 cgcgtaaggg gcggcggcgg cgtattgtgc gtccgatgtg tattcccaaa cggcggtgga
     8221 gagcgtgtgg acatcgtcgg cggtcagcag cagggtggcg gtcaactctt tcatcagctt
     8281 gaggaagacg agtgcgaatg cggcggtaat gccgggcagg atggacggca gtaccaacgt
     8341 cctgaaaata aagaagtgtc cgcgccccaa tgttgcgccg acctgttcca tccctttggg
     8401 gagttgttcc aaggaagtcc tcagggtggt ttgcgccatc ggcaggtaaa gcatgaaata
     8461 ggcaaggatg acgacgataa aggtttggta aacggcaggg gtgtagttga tgctgaaata
     8521 aaccaaggat agggcgataa ccaaaccggg gacggcgtgc agtaaaaacg gcagcctgtc
     8581 tatccaaacg gttaaaaaat tgcgatagcg aaccgatgcc caaacaaggg gcaaggcaca
     8641 taatatagtc aaaatcgcac ctaaagccga tacgcttaag gaacggataa aggcatcaaa
     8701 tacggatacg agcgcgaatg tgccggaagt gccgaccatc atccaatgta tcaatacgcc
     8761 aaaggggata ataatgccca aaatcaacaa gctgcttaaa aaaacaatcg cgccgatctg
     8821 accgggcagt ttgagggttt tgacgggata aggacgggca acgcctttgc cgctgtggta
     8881 aatcttggct ttgccgcgaa atatgctttc tccaaatacg acgatgccgc acaccgccgt
     8941 caaaacagcg gaaagcaggg cggcggtatt gttgttgtag gacatttcgt attcttggaa
     9001 aatggcagtg gtaaaagtgg ggtagttcaa aatggatacc gcgccaaatt cgaccagcat
     9061 atgcagggcg atcagtaaca cgctgctgcc gatggcgggt ttgagctgcg ggaggatggc
     9121 ggaaaaaaag gtttgcaggc ggcttttgcc caaggacagg ctgacttctt cgtaagacag
     9181 gctgatgcgt ttgagtgccg cctcgacggg caggtaggcg agcggaaacg aggacaggct
     9241 cataatcatc actgtccccc aaaagccttc gacgcggaag gtcaggctga tccaggtgaa
     9301 acagctgaca aatgcgggga tgcacaaagg cagggtgatt gccgtctgaa aaaaggtttt
     9361 gccgaagaag cggtaacgtt ggaacaaaag ggcgcaggca atgcccaaaa caatggaaat
     9421 cagggtaacg cccgccatca tcgtcaaggt gttggagagc aaatcccaca tacgcgggcg
     9481 gaacaacagt tcgacggcgc ggttgatgcc gacctgccac gaacgcatag cgacatataa
     9541 aaaaggcagg gtaagcggca gggcgatcag taggatgagg ccggtaagcc aaatgggtat
     9601 ttttttagga gacatagtgt ttttcatcgg caaaacgggc ggacagtata aatgtccgcc
     9661 cgtttgacaa tccgaaaacg gcttatttca taccggcttg ctcaagcagc cgggtggcgt
     9721 gttctttttc ggaaacagtg gtggcggaca cttggggtgc ttccaacttg gcgatgggtt
     9781 ccaaattgaa ggtggatacc acgtgcggat tcaaaggata ttcggcacgg acggcggtca
     9841 gggcgcgctg tccttccttg ccggcgagga aggcgacgaa ttttttcgcc tcatccttgt
     9901 tttgggagga ttttaacacg gctgcgccgg aataggtaac gagtgcgccg ggatctctgt
     9961 ggcggacgaa attcaggcgg gtgtggacat tttgtacgcc tttttcacgc gcgaaagcgt
    10021 gccagtagta gttgttgatg agggcggcat cgatttcgcc gttttcaacc gcttgaaggg
    10081 cgacggagtt tttagcgtaa ggcttgccgt attctttcag gcctttgagc catttcaatg
    10141 cggccgcttc gcctttcagt ttgacgatgg cgacaatctg ttccaagaat gcgccggaag
    10201 tggggacgta accgatgcgg tttttccatt tcggcgtggc gtaattcagg acggattttt
    10261 ccaaatcttt ttcagacagt ttgcgggtgt cgtaaacgac gacgcgcgaa cgtccgctca
    10321 gtgccaccca gtcttttttg gcggcaaccg gcacgccttt gccgcgtgtt tcgttgatgg
    10381 tggaggcggg caggggctct aggaggttgg ctgcggaaag ggtggcgagt gccgggattt
    10441 gttcggaata gaatacgtcg gcggggcttc ggctgccttc ttctttgatt tggccggcaa
    10501 gctggtcgcc tttggcactg ttgagtttga ctttgatgcc ggtagcccgg gtaaaggcat
    10561 ctgcaacggc ttgtgccgct tctttgtgtt ggccgttgta cacggtaatg tctgccagcg
    10621 cgggggtggc ggcggtcagg gctgcggcaa gcagtgcgta tcggatagat gttttcatat
    10681 cgattttctc ctaaatgaat gagggtgtat accttgttaa gacataacgg ggtgtagtgt
    10741 attccttctt ttttataaat gcaaataatt attttttaaa tttgttatta tccgatccgg
    10801 ttattgtttg ttctgacttg tattttttcc gtgcatcgcg cccgtaaggc ggaagcggcg
    10861 ggcaatgcgt ggcggaatgt gggtaaaggc ggcattttga tttgtcggaa tgctggagaa
    10921 cctctccctt taaaacgccg tctgaacaag gttgccggaa tagtattgcc atcccggcag
    10981 atacagtttg tcgggatctt gccaatattg ttcatccaga ctgttcggca gcgaggcggt
    11041 tttgttgtcg agatgttttg ttaatccact atatatccca catttctttt aggtttttac
    11101 cttccgattg gaggtggcgg atttcttttc ccaatgcgtc tgccgcccga tggatcagca
    11161 ttgcgctgtg taccgcgccg atttttttca gaacggaaca agtcttttcg gcggcgggaa
    11221 gaccccagtt gcagacaaat tgcagtatgc cgccgttgcc gatgtcggct tccgcccgcc
    11281 aaatcaaaac cagctcctgt tcccgtccgt ccatgctttc gagtttgccg tcatgctgtt
    11341 caaaaagttt gtccaccgcc tgccgcatca tctgctcgaa gcggtcggct tgggtatccg
    11401 tatcggtcat agtattcttg ccttttaaaa atgccgtctg aacatttctt cagacggcat
    11461 ttgggggtta agctgacatt tcccgccagc gtttgacttg gaggcgtacc tgttcgggcg
    11521 cggtgccgcc caagtggttg cgcgcgttga ggctgccttc gggtgtcaga acgccgtaca
    11581 cgtcgtctga aatcagcttg ctgaagcctt gcagggcttc gagcggcaat tcgctcaaat
    11641 caacgccggc ttcatcggcg tggcgtacgg cttgggcgac gacttcgtgg gcatcgcgga
    11701 agggcatacc ttttttgacc agataatccg ccaagtcggt ggcggtggca aaaccctgca
    11761 tcacggcggc gcgcatattg ccgggtttga cggttacgcc gcgcatcata tcggcgtaaa
    11821 tccgcagcgt gtcgatgagc gtgtcggcgg tgtcaaacag cggttctttg tcttcctgat
    11881 tgtctttgtt gtatgccaag ggctgcgatt tcatcagggt aatcaggccg ataaggtgtc
    11941 cgatgactcg gccggattta ccgcgcacga gttcgggtac gtcggggttt ttcttttgcg
    12001 gcatgatgga cgaacccgta cagaagcggt cggcgatgtc gataaagccg aaacgcggac
    12061 tcatccacaa aatcaattct tcagacaggc ggctcaagtg aaccataacc agcgaggcgg
    12121 cggcggtgaa ctcaatggca aaatcgcggt cggatacggc atcgagcgag ttctggcaga
    12181 tttgttcgaa acccaacaat tcggcggtga tttcgcgctg aatcggatag gtcgtaccgg
    12241 caagggcggc agcgccgagc ggcatacggt tgacgcgttt gcgacagtcg gccatgcgtt
    12301 cgaaatcgcg tccgagcatt tcgacgtagg cgagcaagtg gtgtccgaag ctgaccggct
    12361 gggcgacttg cagatgggta aaaccgggca tgacggtttc ggcgttttgt tccgccaaat
    12421 ccaataatgc cgtctgaagg ttttgaatca ggttttggat aacggtaatc tggtcgcgca
    12481 gccaaaggcg gatgtcggtg gcgacttggt cgttgcggct gcggccggtg tgcaggcgtt
    12541 tgcccgcgtc gccgattttg tcggtcaggc ggcgttcgat gttcatgtgg acatcttcca
    12601 aatcgagcgg ccattcgatt ttgccgctgc ggatttcttc gaggatttcc gacatgcccc
    12661 gacggatgtc ggtcagatcg ttttcactca gcacacccga ccgggtcagc atctgcgcgt
    12721 gggcgagcga gccttggatg tcccattcgg caaggcgttg gtcgaaaccg atggaggcgg
    12781 tgtattgttt gacgagttcg gaaacgggtt cgttgaaacg tccggaccag gttttgtcgt
    12841 gcataaggat tccttgatgg ggttattcgg tgcggtattt ttccaaaagg cggcggaagg
    12901 gttcgccggt ttcggggtgt tttaaaccgt aggcgacggt ggcttcgaga tagccgagtt
    12961 tgctgccgca gtcgtagcgc gtgccttcaa aggggtgcgc caagacaaat tcgtgatcga
    13021 gcagcttggc gatgccgtct gtaagctgga tttcgttgcc cgcgccgcgc ggcaggccgg
    13081 ttaaaaggtc gaaaatgcgc ggggtgagga tgtagcgtcc gacaacggca aggttggagg
    13141 gcgcgtcttc gggcttgggt ttttcgacaa tgccggtaat gcgttggaac tgtttgagct
    13201 gttcggtttc gacgatgccg tatgagccgg tttgcgacgg ttcgacggtt tctacgccca
    13261 aaatgctgtt accgctgcgc ccgtacactt cgaccatttg tttgagcgcg cctttgggcg
    13321 catcgattaa gtcgtcggca aggatgacgg cgaagggttc gtcgccgatg gcggcgcggg
    13381 cgcacaagac ggcgtgtccc aatcccagtg cttccgcctg acggatgtag aggcaggtaa
    13441 tgttcggcgg caggatgttg cggacgtgtt ccaacaattt gtctttatgg cgcatttcca
    13501 acttagtttc gagttcgtat gccttgtcga aatggtcttc gatgctgcgc ttgttgcgcc
    13561 cggtaacaaa caccatttcc gtgcagccgg cttccacggc ttcttctacg gcgtattgga
    13621 tcagcggctt gtcgacgatg ggcagcattt ctttcgggtt ggccttggtg gcgggcagga
    13681 agcgggttcc catccctgca acggggaaga cggctttttt tatcggtttc attctttttc
    13741 ctttgtattg ttttgatgtt taaagggcga gtttgcggat taaatcggcg agtgcctgcg
    13801 cgcggtggct ttcgcggttt ttgacctccg aatccaattc ggcggcggtt ttgccgtgtt
    13861 cgggcagata aaaatacggg tcgtaaccga aaccgttttg cccgagcggc gtgtcgtgcc
    13921 attgtccgtg ccatatgccc tcggcgataa tcgggcgcgg gtcgtcttta tgacggacaa
    13981 aaaccaatac gcagacataa gaacaacttt tgtcggcctt gccggcaagt tcggcggcaa
    14041 gtttcaggtt gttggcggta tcggatttgg gattgctgcc ggcgtaacgt gcggaatgga
    14101 tgcccggcgc gccgtttagg gcggcggcac agatgccgct gtcgtcggcg agtgcgggca
    14161 gcccgctgtg tccggcggcg tgccgggctt ttgccagcgc gttttcgaca aaggtgggat
    14221 agggttcggg gcattcgggt atgccgaatg gggattgcgg caatacggtg atgccgtaag
    14281 gtttgaataa gttgccgaat tctttgagct tgccggcatt gccgcttgcc aaaacgattt
    14341 tttccggttt ttcagacata gcggtttccc tgtgatgaag atggggcggc gcgtagggat
    14401 ttgtgccgca ggtagagggc gagtgcgccg atttgcccga acagcgcgcc gaatgcgaaa
    14461 aggaaggacg cgacggcgaa ggctttgctg ccggcggtca gcagataagc acccaagagg
    14521 acgggcagca taaatgccag tgtgaacaag gcaacagaca acagatagat ttttcggcgg
    14581 ttcatggcgt tcggtcggaa acgggatgtt cggattatag ccgattagga cggcattccc
    14641 tagaggctgg aaaaatgccg tctgaagcgg cgttcagacg gcatggcggg cattatgcct
    14701 gtttgttcca acgttcgatg gattctttga tgactttttt cgcttcttcc gcaccgcccc
    14761 aggattcgac tttggtcgta cccgcttttt tcaggtcttt gtagtggttg aagtggaact
    14821 cgatttgttt gatgagctgt tgcggcaaat cggacaaagt tttgtaggcg ttgccgttat
    14881 tgcggtcgtc ggcaggtacg cagacgattt tgtcgtccac ttcgccgtcg tcgacgaatt
    14941 tcatcacgcc gataacgcgc gcttccaaga atacgccggt tgccagaggt tgttcggtaa
    15001 cgagcaacac gtccaattca tcgccgtctt cgtccaaagt ttgggggatg aagccgtagt
    15061 tggtcggttt ggcgaagatg gcgggttcga tgcggtcgag ttggaatgcg gcgagtttgc
    15121 ggttccactc gattttgtgg ttgctgccgg cggggatttc gttgacgacg ttgatgatgc
    15181 cgccgtccac gtcgcccggg gtcaggattt ggttgaagtc tgccatttgg tttcctttat
    15241 ttgggaaggt ttgaagtttg aaagtatagc acaaacgtcc ggctggaaat gcgtccgatg
    15301 ccgtctgaaa gggaaaccgg gcgcgtgtta ccgtttgccc aaaaacctgc ccagttccaa
    15361 aatcgcgcgc ctgttggacg gggaaaacac tttttccgcc gcttcttcca agccgaacca
    15421 gccgtaggag acgtgttcct cgggttgcag gacgacgggc gtatcgcgcg ggatttcggc
    15481 ccggaagacg tgttcgcggt tttcaaacac gcctttgggg tagcggtgcc gccagtggtg
    15541 gtagatttcg taaaccgtgc tgtcgtgccg gtcttggagc tgcccgtcct ccagcaggat
    15601 gccggtttct tcccaaactt cgcgccttgc cgtttgggcg acggtttcgc ccggttcgag
    15661 gctgccggtt accgactgcc aaaatccttt cggatgcgtg cgttcgatga gcaggatgcc
    15721 gccgtccccg ctataaagga cgaccagtgc ggaaacgggg tatttgagcg gttttgccat
    15781 cggcatcttt cggcgggctg cggtaatgaa ggggcggatt atagcaaacg ccgcacgtta
    15841 tggcgtttat ccttttccgt atcctttttt ctgcacggat gggacgcgcc ggtgtttgcc
    15901 ggtaaatttt cctttgtgtc aaaaagataa gggcggttgt gattttaatg cttgccaaag
    15961 tgacgggcgg aaactataat ccgcgactta ccgagtcgga gtgtggcgca gtctggtagc
    16021 gcacttgcat ggggtgcaag gggtcgaagg ttcgaatcct ttcactccga ccaaaaattc
    16081 cgaaagccgc tttcaaaagc ggcttttttg ccgtccgtat gattatgatg tagagtacgc
    16141 ggcgacagac attcaaatgc cgtccgaaaa ccgttcagac ggcatctctt tatctt
//
LOCUS       DAXDSD010000050        16117 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXDSD010000050 DAXDSD010000000
VERSION     DAXDSD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16117
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.050"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            145..2389
                     /locus_tag="ACFB2Q_001216"
                     /pseudo
     CDS             145..2389
                     /locus_tag="ACFB2Q_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            2514..4064
                     /locus_tag="ACFB2Q_001217"
     CDS             2514..4064
                     /locus_tag="ACFB2Q_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC0918245.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4488..6272)
                     /gene="lpdA"
                     /locus_tag="ACFB2Q_001218"
     CDS             complement(4488..6272)
                     /gene="lpdA"
                     /locus_tag="ACFB2Q_001218"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC0918246.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGSAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(6290..6724)
                     /locus_tag="ACFB2Q_001219"
     CDS             complement(6290..6724)
                     /locus_tag="ACFB2Q_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918247.1"
                     /translation="MSYLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKAT
                     HGPSVKNAVYAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQY
                     SIFEYEVEHPENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(6825..8387)
                     /gene="aceF"
                     /locus_tag="ACFB2Q_001220"
     CDS             complement(6825..8387)
                     /gene="aceF"
                     /locus_tag="ACFB2Q_001220"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC0918248.1"
                     /translation="MSIVEIKVPDIGGHESVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     SLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSASPAAAKIDEAAFAKA
                     HAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKPAAAGASLGG
                     GLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEF
                     RKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADT
                     PNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTG
                     FTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFL
                     ANLLKDFRRITL"
     gene            complement(8537..11200)
                     /gene="aceE"
                     /locus_tag="ACFB2Q_001221"
     CDS             complement(8537..11200)
                     /gene="aceE"
                     /locus_tag="ACFB2Q_001221"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC0918249.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            11897..13609
                     /locus_tag="ACFB2Q_001222"
     CDS             11897..13609
                     /locus_tag="ACFB2Q_001222"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC0918250.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFCPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRGNEAQAVAIGEIVARVTA
                     SLNA"
     gene            13672..14328
                     /locus_tag="ACFB2Q_001223"
     CDS             13672..14328
                     /locus_tag="ACFB2Q_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC0918251.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(14338..14793)
                     /gene="ruvX"
                     /locus_tag="ACFB2Q_001224"
     CDS             complement(14338..14793)
                     /gene="ruvX"
                     /locus_tag="ACFB2Q_001224"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC0918252.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(14786..15334)
                     /locus_tag="ACFB2Q_001225"
     CDS             complement(14786..15334)
                     /locus_tag="ACFB2Q_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC0918253.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(15336..15896)
                     /locus_tag="ACFB2Q_001226"
     CDS             complement(15336..15896)
                     /locus_tag="ACFB2Q_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC0918254.1"
                     /translation="MNRLLLLSAAVLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGTGFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
BASE COUNT         3813 a         4006 c         4321 g         3977 t
ORIGIN      
        1 caatatttaa cagtcggata gctccgcctg attaagtttt tcggtaatag ttgtgattat
       61 caaatataat tgccgcccga aatatccgat aagtttgttt gttttatctt taattcccca
      121 atacttaaaa aagagagcgt tttcatgaac aaaaaacttg ccttaatgcc gctgttgatt
      181 ttgagcgcgt tttcatctgc cgccgacaat actccgcagc atggcgaact cggacaggtt
      241 catgtccgcg ccgatgccaa gcgcgtcaaa gccgcgcatt cttactccat cgccagcgat
      301 ggcgacttgc gcgaccgcgt aaatttgggc gtattgggta aagccaacgc gtttaccgcg
      361 ccgattaccg tcgtcaacta cgacgaacaa gccttcaaca ataccgaggc gcgtacttta
      421 gtggatgccg tagccaaaaa agacgcttcc gtttgagagt ttggcggcga aagcaacacg
      481 ctgaccggtc tgtatttccg tggttatcag cttgatgcgc gccaattcag cgtaaacggc
      541 ttggcaggaa tgtatggcac gcaaggcacg gcgagcgtgc aagtcggctt cgcacaactg
      601 attaaaggcg cgtccaccgc cgtaaacggc ataccctgaa ggcgcggtat ccggttccgt
      661 caacatcgaa accaaaaaag ccgccgacga aggcaaccgc aaaatcggtt tggcccggtt
      721 cggcaacaac cgcgcccaag gtaggttcga cttagggcaa cgcttcggcg aaaacaaagc
      781 attcggcgtg cgtgccaacg gcaaactgcg ccacggcgac accccgcgcc acggttacag
      841 agaagacaac aaagaatttg ccttaaatgc cgattatcgc ggcgaaaaac tgcgcgtgac
      901 gttcgactcc atctacgcga aacgcaaaat caacggcggc cgcgcgcgta tgcaggatat
      961 ccaaaacgcc ggcggacgct tgttcgacgc gcccgacggc aaaatcaacc tgctgccttc
     1021 ttggaactgg caaaataccg tcggcgaaac caatatgctg acgtttgaat gggatgcgtt
     1081 tgacaatacc taaattaccg gcggtatcgg ctacaacaaa gcgcgttatt acggtacgct
     1141 gatttcccca accgtttgcg gtacaagcgg tgcgagcagc caaaccaaaa cctgcatggc
     1201 tgccaaccaa taccacaccg gcacggcacg cctgaccgac caatatttcc gtactttgag
     1261 tatgaacctg accgcacgcg gcgaatttga aaccggcccg gtcacgcata actggagtac
     1321 ggctttcgac cgcgttatcc gccagcggaa aaccataaac ggcagcggga acggcaacag
     1381 caagatagaa gtgaaggcaa acgaaaatat tgcgaatcaa ttggcatcgt tcaaagccga
     1441 ctatcccaat tcttgggcga actcggccaa cttggatgcc aatatcaaag tcaacagctt
     1501 ggctttgtcc gatactttgg gcttcgttga taacaaatac cccctgactt tgggcggacg
     1561 tttccaagcg gttgaataca ctgataagaa gaaatcacag agcggcgatg ccaaacgttt
     1621 cagcccgatg ctgatggcgg cgtgggtgcc gcaacctgat ttggtcgtgt gcggcaacta
     1681 tatggaagac ttggagcgtg ccgatatcaa aaccgatgat tccggcgaaa cgacaatggc
     1741 gaagccgcgc gtcagccgcc agtttgaaat cggcgtgcgt aaaaactggg gcgattttgt
     1801 aaccacgttg aacgcgttcc aaatcaaacg cccgggctac tggcgcggca ataccaaaaa
     1861 aggtactgat tttgcagcat ataaagcagc aggaggcgca gccggcgacg agcaaggtat
     1921 ggaacgcaat cgcggtattg agttcaatgc ctatgccaac ctgttgaaca aaaccctgcg
     1981 cccgactttc ggcgtgatgt atctgcaatc gtccgtgaag aattatccga atgcgcgcga
     2041 tatgctggtt aacggcgtac aagtcgccaa cccgcgcgtg attgtcaaaa caggcgttga
     2101 acgggataca ccgtttgcca aaggtttgag cttgaacggc aatgtttcgt atttcggcaa
     2161 gtcttatcaa gacacgcaaa aacaatacgc cttcccgtcc tataccttgg ttgacgtagg
     2221 cgcgcgctac aaaaccaagc tgggcaaaaa caccctgacc gtcagcagct cggtagaaaa
     2281 cctgttcaac aaaaactact ggcaggtaca gcgcggacaa tttgaccgta gtttcgccgt
     2341 cgtcggtctg ccgcgtactt actggctgaa ggcggaattg gatttctaag ctgcctccgt
     2401 ttccgaaaat gccgtccgaa caggtttcag acggcatttc ttttaaagaa atatggcggg
     2461 atatgcttaa ataatgctat tctagtacct ttgaaacctg cttgtttgaa gtgatgaaaa
     2521 aacctttgat ttcagttgcg gcagtattgc tcggcgttgc tttgggtaca ccttattatt
     2581 tgggtgtcaa agcagaagaa agtctgacgc agcagcaaaa aatattgcag aaaacgggct
     2641 ttttgaccgt cgaatcgcac cagtatgatc gaggctggtt tacctctacg gaaacgacgg
     2701 tcatccgtct gaaacccgag ttgctgcata atgcgcagaa atacctgccg gataacttga
     2761 aaatagtgtt ggaacagccg gttacgctgg taaaccatat cacgcacggc cctttcgccg
     2821 gcggattcgg cacgcaggcg cacattgaaa ccgagttcaa atacgcgcct gaaacggaaa
     2881 aagttttgga acgctttttt gggaaacaag ttccggtttc ccttgccaat accgtttatt
     2941 tcaacggcag cggtaaaatg gaagtcagtg ttcccgcttt cgattatgaa gaactgtcgg
     3001 gcatcaggct gcactgggaa ggcctgacgg gggaaacggt ttatcaaaaa ggtttcaaaa
     3061 gctaccgcaa cagctatgat gcgcccttgt tcaaaatcaa gctggcagac aaaggcgatg
     3121 ccgcgtttga aaaagcgcat ttcgattcgg aaacttcaga cggcatcaat ccgcttgctt
     3181 tgggcagcag caatctgact ttggaaaaat tttcgctcga atggaaagag ggtgtcgatt
     3241 acaacgtcaa attgaacgaa ctggtcaacc tcgttaccga tttgcagatc ggcgcgttta
     3301 tcaatcccaa cggcagcatc gcaccttcca aaatcgaagt cggcaagctg gctttttcaa
     3361 ccaagaccgg ggaatcgggc gcgtttatcg acagcgaagg gcggttccgt ttcgatacgt
     3421 tggtgtacgg cgatgaaaaa tacggcccgc tggacatcca tatcgctgcc gaacacctcg
     3481 atgcttctgc cttaaccgta ttgaaacgca agtttgcaca aatttctgcc aaaaaaatga
     3541 ctgaggaaca aatccgcaat gatttgattg cggcagtcaa aggcgatgct tccggattat
     3601 ttacccatga cccggtacta aatatcaaaa ttttccgttt caccctgcct cagggaaaaa
     3661 ttgatgtggg cggaaaaatc atgtttaaag gcatgaagaa ggaagatttg aaccaattgg
     3721 gactgatgtt aaagaaaacc gaggcaaaca tcagaatgag tattcctcaa aaaatgttgg
     3781 aagatttggc ggtaagtcag gctggaaata ttttcagtgt aaatgccgaa gatgaggcgg
     3841 aagccagagc aagcattgcc gatattaatg aaacattgcg cctgatggtg gacagtacgg
     3901 tccaaagtat ggcaagggaa aaatatctta ctttagacgg taatcagatt gatacggtca
     3961 tttcccttaa aaacaacgcc ctgaagttaa acgggaaaac gctgcaaaat gaacccgatc
     4021 ctgattttga cgagggagat atggtttccg gccagccgca ttaatgccgt ttatgccaaa
     4081 tgccgtctga aatgttcaga cggcattttt gtcggtctcg aaataggatg gattttcatg
     4141 tttcaaaaac aattgattgt ctctaatccg atatcaggac acgcttccaa tgtttagata
     4201 tggaattcgg acgggatagg ggaactattg aaatccagtt gaaatctgtt attggaaagc
     4261 atggaggaaa aataaaaggc tgtattttaa tatagtattg ataggcatgc ttagcttcaa
     4321 ggtattcaat agtgtaggtg tctgcctaac tggaaaataa tcgtcttttc ccttgcaacc
     4381 agttaaaatt gacagatggt taaaacacag ccaaataaaa atgccgcctg aagggttcag
     4441 acggcatttg ttcggataaa ccttatcggc tgcttagagc atcaggttta ttttttcttt
     4501 tgaggaggca ggtcggtaca tacacccaaa gccacttccg cagccatacc gatggattcg
     4561 cccaaggtcg ggtgcgggtg gatggttttg ccgatgtctg ccgcgtcgca gcccatttcg
     4621 atggcaaggc agacttcgcc gatcatgtcg ccaccgttcg gaccgacaat gccgccgccg
     4681 atgatgcggc cggtttcggc atcgaaaatc agcttggtaa agccgttgtc gcaaccgttg
     4741 gcaatcgcac gaccggaagc cgcccatggg aagttggctt tggtgatttt gcggcctgat
     4801 gctttggcag acaattcggt ttcaccgacc catgccactt cgggggaagt gtaggcaacg
     4861 ccgggaataa cgcgtgcgtc gaagtaggct ttgtggccgg cacagttttc agcggcaacg
     4921 tgaccttcgt gaacggcttt gtgtgccaac atcggttgac cgacgatatc gccgatagcg
     4981 tagatgtgcg gtacgttggt gcgcatttgt ttgtcaactt cgatgaagcc acgatcggta
     5041 acggcaacac cggctttttc tgcgctgatg agtttgccgt taggcgcacg gcctgccgcg
     5101 accagtacgg catcgtaacg ttgcggctct ttaggtgcat ttgcaccttc aaaggtaacg
     5161 taaacgccgt cttctttcgg ctcgactgca acggttttgg tgttgaccat aatgttgtca
     5221 aaacggtatt cgttttgttt ttgccatact ttaaccaagt cgcggtctgc gccttgcatc
     5281 aggccgtcca tcatttcaac cacatccagg cgagaaccca gcgtgctgta aaccgtaccc
     5341 atctcgaggc cgataatgcc gccgccgata atcagcagtt tgcccggtac ttctttcagt
     5401 gccagtgcgc cgctggaatc gatgatgcgc ggatcttcag gaatgaaagg cagtttggtt
     5461 acgcggctgc ctgctgcaat gatacagttt ttgaaggcaa cgattttttt ctcgccggta
     5521 ggggctgcct gttcgtacgc gtcgccggca gtcagcgaca cttccaagtg gtgaggatcc
     5581 aagaattgcc cgtcgccttg gataacgtcc actttacggc ttttcgccat acctgccaaa
     5641 ccacccgtga ggcgggaaac cacgccgtct ttataggcgc gaagcatatc gatgtcgagt
     5701 tccggttcgg ggtatttgat accgttggca gccaagtggc gcacttcgtc gataacggcg
     5761 gcattgtgca acaaggcttt ggaagggata cagccgacgt tcaggcaaac gccgcccaaa
     5821 gttttgtaac gctcgacgat ggcgactttc aagccttcat cggcagcggc aaatgcagcg
     5881 gagtaaccgc cgggaccgcc gcccaatacg accacgtcgt actcggcatc ggcagcactg
     5941 ccgaattgcg cggcttgcgg agcaggagcg gcagctttgg gtgcttcttg cgccggggcg
     6001 gcagccgctt cggctttagg ggcggcagcc gcaccttcgg cttcgacgac gacaatcaaa
     6061 ccgccttcgg agattttgtc gccgactttg actttcactt ctttgatcac gcccgcaact
     6121 tcggcaggca cgtccatcgt cgctttgtcg gtttccaagg taatcagggt gtcgtcaacg
     6181 gcgatggtgt cgccaacgtt cacttcaacc gcgataatat ctacattttc gtgtccgcca
     6241 atgtcgggca ctttcaattc aactaaagcc attatattga cctttcaact tagttaattt
     6301 ctatcaactc tttagcaata atcacttctt caggaataca gccacaatct tcagctctga
     6361 ttgttacttc cttctcatct gggttttctg gatgttcaac ctcatattca aaaatagaat
     6421 attgaccaaa caaatctcta tttaaaacat atatatagcc attttcgatg ccggagcttg
     6481 ttgcaaattt cttggcaatt tccttgtctg ttgtcgtaga tatataacat ccgtcatata
     6541 gatctgtttc aatttgatgg gcgtaaactg cattcttcac acttggacca tgtgtagctt
     6601 taccatcata tttaaactta ccatcataac gaattgcaac ttcagcttta ttacctttag
     6661 gttttaactg tccattattt tgctcatctt gttggcaact aatgcctcta tataaataag
     6721 aaatttccca taagttatta atttaaagtt tgaatttaac accatcactc atcaatctca
     6781 agcaatccaa acattatcag actgcttgag agggatgttt tattttataa ggtaatgcgg
     6841 cggaagtctt tcaacaggtt cgccaggaat acggtgaagc gcataccggc ggcaccgtcg
     6901 atgacgcggt ggtcgaagga caggctcaac gggcacatca ggcgcggggc aaactctttg
     6961 ccgttccaaa ccggtttgat ttgggatttg cacacgccca agatagcgac ttcgggagcg
     7021 ttcacaatcg gcgtgaaacc tgtgccgccg atgccgccca agctggaaat ggtaaagcac
     7081 gcgccttgca tttcttgcgg tttgagcttg ccttcgcggg cttttttgga caattcggtc
     7141 aattcttggc tgatttgttt caagcctttt tgatccacgt ctttgatgac gggaacaacc
     7201 aagccgttcg gcgtatcggc tgcgaaaccg atgttgaagt agtttttcag caccaggttg
     7261 tcgccgtcca aagaagcgtt gaattcgggg aaggctttca gcgcggaaac ggaggctttg
     7321 atgatgaacg ccaacgggga cagtttcacg ccttcgcgtt cccattcttt gttcagctgt
     7381 ttgcggaatt cttccagttc ggtcatatcc gcttcttcgt gtacggtaac gtgaggaatc
     7441 accacccagt tgcgggacag gttttgaccg gagattttct taatgcggga caattcttta
     7501 acttcgacat tgccgaattt ggagaagtcc actttaggcc acggcagcaa gtccagaccg
     7561 ccgcccaaag atgcgccggc tgcggcaggt tttgccgcgc cgccctgcat tacggatttc
     7621 acaaaggctt tgatgtcgtc tcccatgata cggcctttca agccgctacc tttgacttgg
     7681 cccaaatcca cgcccaattc gcgcgccagt ttgcgtgcgg aaggaccggc gtgtgctttg
     7741 gcgaaagcgg cttcatcgat tttggcagcg gcaggcgagg cagatgtagg cacggcggcg
     7801 ggtgcggcag cttgagcggc aggcgcagga gctgctgcgg cagagccggc ggtttccact
     7861 tcgataatgg cagagccttc ggacactttg tcgccgactt ttaaggatac ggctttaacg
     7921 acaccggcag cggtacaagg tacgtccatt gtcgctttat cggtttccaa agtaatcagc
     7981 gtgtcgtctt cggcaacggt gtcgccaact ttgatttcaa cggcgattac atctacatcg
     8041 gtatggccgc cgatatcggg aacggctact tgaacggttg caccgcctgc ggcagcgggt
     8101 gcaggttgtg cttcggcggc agcagccggg gcggcttcgg cagcggcggc accggtttca
     8161 acggtcaaaa ttacgccgcc ttcggagatt ttgtcgccga ctttgacttt tacttctttc
     8221 acgacaccgg ccgcatcggc aggcacatcc atcgtggctt tgtcggtttc cagtgtaatc
     8281 agggtgtcgt caacggcgat ggtgtcgccc gctttaactt ctacggcgat gatgtcgacg
     8341 ctttcgtgac cgccgatatc ggggactttg atttctacga tactcatttg agttccttta
     8401 atctttttca gttggatgcc gtctgaaagc tgcttcaggc ggcatcggta cggtttgtct
     8461 gttcaaacca ttcgggcatt cggttttcag acggcctgat gttggcatga aacaaacagg
     8521 ccgtctgaaa catcaatcag cgtttccagc taggggctga atcggctttg atgccgtatt
     8581 tctcaatggc ttgttgaacg gtttctttgc tgactttgcc ttgttccgcc aatgcggcca
     8641 atgctgccac ggcaacgttg tagcggtcca cttcaaagaa gcggcgcagg ttggcgcggc
     8701 tgtcggaacg gccgaaaccg tcagtgccca agacatggta gtcgttcggg atgtacgcgc
     8761 ggatacggtc ggcatagctg cggatatagt cggtagcggc gataaccgga ccgtcatgac
     8821 cttgcagttg ggaagtaacg aaaggcactt tttcagcttc cagcggatgc aggcggttga
     8881 agcgttcggt ttcgatggcg tcgcggtgca gcaggttgaa ggacgggcaa gaccagatgt
     8941 ctgcttccac gccgaagtcg gctttcagca gctcggcacc ggcaatcact tcttgcagaa
     9001 tcgtaccgga acccatcaat tggactttct tgtcgccttt gccgccggct ttcagcaggt
     9061 acataccttt gaggatgtct tgttccgcac cttccggcat atcgggatgg gtgtagttct
     9121 cgttcatcag ggtgatgtag tagaacacgt cttcattatt ggcatacata cggcgcagac
     9181 cgtcttgtac gatgacggcc acttcgtatt ggaaggtcgg gtcataagat acgcagttcg
     9241 gaatcaggtc ggcctggatg tggctgtggc cgtcttcgtg ttgcagacct tcgccgttca
     9301 gcgtcgtacg gccggcagta ccgcccagca ggaagccgcg cgcgtgcata tcgcccgccg
     9361 cccaagccaa gtcgccgata cgttggaaac cgaacatgga gtagtagatg tagaacggaa
     9421 tcatcgcgaa gtcgctgttg gcgtagctgg tcgcagccgc aatccagtcg gccatcgcgc
     9481 ccggttcgtt aataccttct tgcaagattt gaccgtcgac ggattctttg tagaacatca
     9541 gttggtcttt atcttgaggc gtgtattgtt ggcctttggg attccaaata ccgtattggc
     9601 ggaacatacc ttccatgccg aaagtacggc tttcgtcggg aacgataggt acgacgcgtt
     9661 tgccgatttt tttgtctttc agcaaagtgg acaggatgcg gacgaatgcc atcgtggtcg
     9721 agaactcgcg ttcaccgctg gatttgagtt gtgcgtcgaa tgccgacagc tcgggcactt
     9781 ccaatacttc ctgcgtcggt ttgcgttgcg gcaggtagcc gcccaaagca tcgcggcgtg
     9841 cgtgcaggta tttgtattct tccgtatcgg gggcaaaagt caggtaaggc agatcgccgc
     9901 tttcgatttg ttcgtcggta accggaatgt caaagcggtc gcggaattgt ttcagggacg
     9961 ctttgtccat ttttttggct tggtgggcaa cgttctgacc ttcgccggat gcgcccatac
    10021 cgtaaccttt aatggttttc gccaagataa cggtaggttt gccgtcggca tggttcgctg
    10081 cgcggtcgta ggcgttgtac actttttgag gatcgtggcc gccgcggttc aatgcccaga
    10141 gttgctcatc ggtcatatcg gcaaccaatg ctttcagttc gggcgtattg aagaagtgtt
    10201 cgcgcacata cgcgccgtct ttggatttgt aagtttggta gtcgccgtcc aaacattctt
    10261 ccatacgttg gcgcaggata ccgtctttgt ctttcgccaa gaggcggtcc caacggcggc
    10321 cccaaatgac tttgacgaca ttccagccgg caccggcaaa gttgccttcc aattcttgga
    10381 tgattttgcc gttgccgcgc accggaccgt ccaagcgttg caggttgcag ttgatgacga
    10441 aaatcaggtt gtccaagcct tcgcgtgcag ccagcgcgat tgcaccttgg gattcgggtt
    10501 cgtccatttc gccgtcgccg cagaaacacc atactttacg gcctttggtt tttgccaagc
    10561 cgcgtgattc caagtatttc aggaaacgcg cctgataaat cgccatgatg gggcccaagc
    10621 ccatggatac ggtcgggaac tgccagaagt cgggcaagag gtgggggtgt ggataggaag
    10681 gcagaccgtg tccgtccact tcttggcgga agttgttcag ctggtcttcg gtcagacggc
    10741 cttcgacgaa tgcgcgtgca tagatgcccg gggcaacgtg accttggaag aagaccaaat
    10801 cgccttcttc accttcacct ttggctttcc agaagtggtt gaaaccgact tcgtacatgg
    10861 tggcggcaga ttggaaagat gcgatgtgcc cacccagttc caaatctttc ttgccggcgc
    10921 gcaatacgat ggcggcggcg ttccagcgta cgaatgcgcg aatgcggtgt tcgatgtttt
    10981 ggtcgcccgg aatgcctttt tcgttttcaa ccgaaacggt attcaaatac ggggtggtcg
    11041 tgccgtgggg catacgtacg cctttgtcgc ggcagtattt gagcaggttt tccaagaggt
    11101 attgcgcgcg ttcgccgcct tcatattcga ggacggagct taacgcgtcc agccactctt
    11161 gggtttcgat agggtcaaca tcgtgtaatt gggtggacat agtatctatc ctttatgttg
    11221 agtgtttaat cggaagggag ggtgtagcca acccctttat tttcgtttgt gaaaaataat
    11281 aagcaaaaac gaaaatttct aaatcaaacc gaaacaaaat aatttaagtt taatgttttc
    11341 aaaaaattat aaaagtcttg gaagcaaggg aaaacttgaa aaaaaatgtt cggtctgctc
    11401 cgcacgaaat cctatcagca atcgggtctg ctggcaaata accatgcttt taaaagcagg
    11461 ttttatactg aagttggcgt ttggtaagga caggtctttt tgattgcgaa aacattctgt
    11521 ttaattttgg ttatattttt tcggaattgt gggatatcga atcgttatgt ctctgtcggc
    11581 aattttccta ttgcagaaaa tctatacaca agctttgtgg acaaccgtgt tgataagcca
    11641 cggcttgtgt taaaaaagtc ttacgcaaca aatgtacgct tattttgatt aaaaattagg
    11701 caaatttaaa tctataaaaa tcatgtaatt atgttcaata tgatgtttct ggtcatgttt
    11761 ttgccgtctg ttatggggtt tgtcccgcgt ttggggcagg gggtgaattt tggaggttgg
    11821 tgcaaaacct gcttttttcg cgttacaatc tgcacccgat ttacttttga atctgcctgt
    11881 ttggaaaaat acttttatga aagccagcca attctttatc tctactttaa aagaagcccc
    11941 tgccgaagcc gcgtttgcca gccacaagct gatgattcgc gccggtctga ttaaagccaa
    12001 cgcgtccggt ctttatactt ggatgccgat ggggctgcgc gtgttacgca aagtcgaaaa
    12061 cgtcgtgcgc gaggaaatgg cgcgcgcggg cagcgtggag ctgctgatgc cggtggtgca
    12121 gcctgccgaa ctgtggcagg aatccggccg ctgggagttt tacggtaaag aactgctgcg
    12181 cctgaaagac cgccacgaac gcgatttctg catgggcccg acctgcgagg aagtcatcgc
    12241 cgacatcgtg cgcaaagaaa tcaacagcta caaacaactg ccgaaaaatt tttaccacat
    12301 ccaaaccaaa ttccgcgacg aagtgcgccc gcgtttcggc gtgatgcgcg cgcgcgaatt
    12361 tgtgatgaaa gatgcttatt ccttccacgc cgactacgcc tcgcttcagg cgacctatga
    12421 tgccatgtat gacgcttact gccgcatctt tacccgtctg ggtttggcgt tctgccccgt
    12481 cgccgcagac accggcagca tcggcggtac gggttcgcac gagtttcaag tgttggcgga
    12541 aagcggcgaa gatgtgattg catacagcga cacttccgat tacgccgcca atatcgagtt
    12601 ggcaccgacc ttgccgctta aaggtgaacg tgccgccgct caggctgtgt tgaccaaagt
    12661 acatacacca aacgtcaaaa ccattgagtc tttggttgaa ttcctgaata ttccggttga
    12721 acaaaccctc aaatccatcg tggttgaagg cgaaaacgaa ggcgaactcg tcctactgct
    12781 gttgcgtggc gaccatgagt ttaacgacat caaggcagaa aaactggcgg gcgtaaaatc
    12841 gccactgact atggcaagcc ctgccgcgat tgttgaacaa ttcggcgcaa acggcggttc
    12901 gctcggcccc gtcggcttca caggcaaagt ctatgccgat ttcgctaccg aaaaaggcgc
    12961 ggactgggtt atcggcgcaa acgaagacga ctaccactat accggcttca acttcggccg
    13021 cgatgccgcc gagcctgaat tcgtcgattt gcgtaatgtg gtcgaaggcg acgaaagccc
    13081 cgacgggcaa ggccgtctga aactggcgcg cggcatcgaa gtcggacatg tcttccagtt
    13141 gcgcggcaaa tatacccaag ctatgaacgt cagcttcctc gacaataacg gcaaatcgca
    13201 aatcatggaa atgggctgct acggcatcgg catcacccgc gtcgttgccg ccgccatcga
    13261 gcagaataac gacgaaaaag gcatcatttg gaccaaagcg atggcgccgt ttgaagtcgt
    13321 catcgtgccg atgaactaca aaaaatccga cactgtgcgt gaagccgccg acagaatcta
    13381 tgccgaattg ctggcggcag gcgcggatgt gctgctggac gaccgcgacg aacgcgcagg
    13441 cgtattgctg aacgattccg agcttctcgg tatcccgcac cgcatcgtca tcggcgaccg
    13501 cgccttgaaa gaaggcaatg tcgaatacgc cgaacgccgc ggcaacgaag cgcaggcagt
    13561 tgcaatcgga gaaattgttg cgcgtgtaac agcttcatta aatgcgtaag tctggtcaaa
    13621 tgccgtctga aaataatttc agacggcatt tttattttgg gggaaagtga agtggcttca
    13681 gtatttttag aaggagaagc agtcgaggtc ggcaatattt tctgtatcgg caggaactat
    13741 gccgcacatg tcgaagagtt aaaaaacgaa ataccttcag aacctgtggt gtttatgaag
    13801 ccgtcaggca gcattttgaa cagcggcggt acaatcttgc tgcccgagtt cagtagggat
    13861 gtgcagtttg agtgcgaact agtgttgctg attggccggg attcagacgg cacgggcgag
    13921 ggtgaagata ttttgggatg cgtggcgggg tacggcgtgg gtttggattt gaccgcgcgg
    13981 gacatccaat gccgtctgaa ggcgcagggg ctgccctggc tgaaggcaaa agggttcagg
    14041 cattctgcgt gcgtgtcgga ttttgcggcg gcagacagaa tcggcaatca agataaggtg
    14101 ttgttttcat taaaacaaaa cggcgtattg aaacagcgcg gggagactgg tttgatgatt
    14161 tacccgattc gggaaatttt acacaaactg gcggcggatt acggtttggg gaaaggggat
    14221 ttggtgttta ccggaacgcc ctccggcgtg ggcgcaatcg gtgcggggga caatttggca
    14281 ttggagctgg acggcttggt gtgcgcctct ttcacgatag ggtgttaacc gcgccgctta
    14341 accttcacgc ccgttgaaac attccgccgg gccgccctcg agaaaaccgt gcaggatggc
    14401 ttgcgccgcc acttggtcga gcaccgattt gcgttttttg cccaagacct gtgcttccga
    14461 aagcaggctt tcggcataga cggacgacag ccgttcgtca acccaataga cggggagatt
    14521 gaacctgccg ttcagcctgc gtccaaactt gcgcgacagg tgcgtcattt catgttccgt
    14581 gccgtcggca tgcacgggca gtccgacgac aaaataacgc ggctgccatt cttgaaccag
    14641 cttggcgatt gccgcgaatt tttcatcgtt gctgccgccg gtaacggtgg ccagcggatg
    14701 ggacagcccc aattccgcat cgccctgcgc cacgccgatg cgcgcttcgc cgaagtcgaa
    14761 tgccagtgcc gttccttttg gaattttatg catggccggc tcccgaaaac agggcgagcg
    14821 ggtcgatgcc gagtttggcg aatgcggcgg cgtaacggtg ttcgtagggg atgtcgaaca
    14881 ggatgtgttc gtcggcggga acggtcagcc acgcattgtc ggcaagttcg cgttcgagct
    14941 gccctttgct ccagctcgaa tagcctatgc tgatcaaggc tttgtcaacc gcgccttcgc
    15001 gtgaaatgtt ttcaagcaca tcgcgggaag aggtcagcgc gatgccgtct gaaacgccga
    15061 tgctgctttg ccagttgccg atcggagtgt ggacgacgta accgcgctcg acctgcaccg
    15121 gcccgcccat catcacgctg tcgtgctgca tccgcatggg gatgtttttg ccggtggcgg
    15181 aaaaaatcat gtccatcgta atcggagagg gtttgttgat ggcgataccg agtgcgccgt
    15241 cttcatcgtg tttgcagata tagacgaccg attgtgaaaa aaacgcgtct tccatatcgg
    15301 gcatggcgac gagaaagtgg ttggacaggt tcatatcagc gtttgtcggg attttttatc
    15361 gggttggaaa taatgcaggc gccggcaagc agtttgccgg aagtatcggc ttgcaccccg
    15421 tcggtttgcg gtatgccgct gccgaaacag gaaaccgcgc ttaaggaatt tttcggcgat
    15481 ccttggacga ttttatcgct gtaaaccaaa taggcgaagg ctttgcgttt ggggtcgtaa
    15541 taacggacaa tctgccggct cttgaacgcg aagcccgtac cgcgcttgaa aacttctttc
    15601 ggtttgcgca cggcggtttc gtcaaaagaa atcgaagatg ccgtctgaac gcacgaaacc
    15661 gatgcgtcgg acgcgtcctc ttccaaattg accatttcct tcaagccgcc tttttttgca
    15721 tacgaaatat aacaggcaac cccttgaacg tcgggatcgt cgaatccttc cacttcgata
    15781 cggtcgtttt tgcccaacat gttgaaaacg gtacttgccc gtccgatttt atcggtttcg
    15841 ccgctgccgc aggcagtcgg caggacggcg gcagacagca gtagcagtct gttcatcaaa
    15901 atttccttat cggttgaatc cccgttttcc ggaaggcggc agggtttgct ttgtttggag
    15961 gggcgcacgc tcccaaatcg ggcaacatac ggtacgcagc ttacatgtgc taccctgtgt
    16021 gtcggaacac aatatccgtt gcagcattga aaatatgccg cacaatataa ggctgtttga
    16081 gccgattgca agtatccgcg caaagaaggt gcaagat
//
LOCUS       DAXDSD010000051        16100 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXDSD010000051 DAXDSD010000000
VERSION     DAXDSD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16100
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.051"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(120..1262)
                     /locus_tag="ACFB2Q_001227"
     CDS             complement(120..1262)
                     /locus_tag="ACFB2Q_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC0918255.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQAGSAPAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTVAITAKGK
                     ELLGGKLPENGWDLLFKPEYTRKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1568..1831
                     /gene="rpsT"
                     /locus_tag="ACFB2Q_001228"
     CDS             1568..1831
                     /gene="rpsT"
                     /locus_tag="ACFB2Q_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC0918256.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            1991..3139
                     /locus_tag="ACFB2Q_001229"
     CDS             1991..3139
                     /locus_tag="ACFB2Q_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC0918257.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            3281..3982
                     /locus_tag="ACFB2Q_001230"
     CDS             3281..3982
                     /locus_tag="ACFB2Q_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC0918258.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            4040..5848
                     /gene="aspS"
                     /locus_tag="ACFB2Q_001231"
     CDS             4040..5848
                     /gene="aspS"
                     /locus_tag="ACFB2Q_001231"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC0918259.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGIAGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVATGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLRLRQKAVETKEA"
     gene            5998..6282
                     /locus_tag="ACFB2Q_001232"
     CDS             5998..6282
                     /locus_tag="ACFB2Q_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC0918260.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6449..8341
                     /gene="speA"
                     /locus_tag="ACFB2Q_001233"
     CDS             6449..8341
                     /gene="speA"
                     /locus_tag="ACFB2Q_001233"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC0918261.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8440..9363
                     /gene="speB"
                     /locus_tag="ACFB2Q_001234"
     CDS             8440..9363
                     /gene="speB"
                     /locus_tag="ACFB2Q_001234"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC0918262.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9561..11027
                     /locus_tag="ACFB2Q_001235"
     CDS             9561..11027
                     /locus_tag="ACFB2Q_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC0918263.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFVMSVVNFLIFSVIGSIWWKVLGYW"
     gene            complement(11231..12307)
                     /locus_tag="ACFB2Q_001236"
     CDS             complement(11231..12307)
                     /locus_tag="ACFB2Q_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFC0918264.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            12371..12508
                     /locus_tag="ACFB2Q_001237"
     CDS             12371..12508
                     /locus_tag="ACFB2Q_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFC0918265.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGWFR
                     L"
     gene            12530..13672
                     /gene="bioF"
                     /locus_tag="ACFB2Q_001238"
     CDS             12530..13672
                     /gene="bioF"
                     /locus_tag="ACFB2Q_001238"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC0918266.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            13682..14329
                     /locus_tag="ACFB2Q_001239"
     CDS             13682..14329
                     /locus_tag="ACFB2Q_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC0918267.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            14317..15099
                     /gene="bioC"
                     /locus_tag="ACFB2Q_001240"
     CDS             14317..15099
                     /gene="bioC"
                     /locus_tag="ACFB2Q_001240"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC0918268.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            15341..16100
                     /locus_tag="ACFB2Q_001241"
                     /pseudo
     CDS             15341..16100
                     /locus_tag="ACFB2Q_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
BASE COUNT         3959 a         3871 c         4167 g         4103 t
ORIGIN      
        1 ctataaatgt ccgcaatctg aaatcccggc cgtgggggcg acggtataaa tatttctatt
       61 tccatccaat ataaaatgcc gtccgaacat cgttcggacg gcatttttaa tgcttcgaat
      121 cagttggtgc cgactttgat tttctgccac aggctgaccg acagtttttt cgcatccgtg
      181 ctcatttgcg gcatgacgaa accgtctttc atatcctgct cgttcgggaa gatggaacgg
      241 gtgttcacca actcggcagg cattttttcg cgcgccggtt tgctggcggg ggcgaaggtt
      301 acggcgatgc cgtttttcgc cgcgatttcg gggtcgagcg tgtagttgat gtatttgtgg
      361 gcgttggcga cgtttttcgc atcggcggga atcagccaag actcaatcca gaagcccata
      421 cctttcggtg tcagcacttc gatgccgacg ttgtttttca cttcttcgga acgcgctttc
      481 gccaagttca aatcgccgcc gttgcctgcc gccaggcaga tgtcgccgcg cgccagctcg
      541 tcgatgatgg acgggctgaa acgtttgacg tccggacgga tagacttcaa cacttccgcc
      601 gccgccttca aatcttcagg attcgaacct ttggggtctt tgcccaagta gttcagcaaa
      661 atcgggaaca tttcgctcgg ggtgtcccac aaggcgatgc cgcaggattt cagcttgcgg
      721 gtgtattcgg gtttgaacag caaatcccag ccgttttcgg gcagcttgcc gcccaaaagc
      781 tctttgccct tcgccgtaat cgcaaccgtg ttcacgccgg agaaataggg gacggcatac
      841 tggttgcccg ggtcggcggt ttccagcatt ttcaagagtt cgggatcgat gtttttatag
      901 ttgggaatca ggtctttgtt gactttttga tacgcgcccg cctcgatttg gcgcggcagg
      961 aaggcgatgc ccggcacaac caaatcgtaa ccggatttgc cggtcagcat tttggcttcc
     1021 agcgtttcgt tgttttcgta caagtcgtaa gtcagcttca gattgttggc ttttttaaag
     1081 tcttcgaccg tactctcgtc aacatagttc gaccagttgt agatgttcag agtatcggtg
     1141 gcagcggctt cggcattggc agcaggtgcg ctgcctgctt gaggctgcac ggcgtttttt
     1201 tcgctgccgc cgcaggctgc cagagacagc gcggccaaaa cggctaatac ggattttttc
     1261 atacgggcag attcctgatg aaagaggttg gaaaaaaaga aaaccccgcg ccccatagac
     1321 accccggcgc aaggtttggg cattgtaaag taaatttgtg caaactcaaa gcgatattgg
     1381 cctgattttc ctaaaaaatt accctgtttc caaaaaaggg ggggggaaac ggccgcccga
     1441 ttttgccgtt tttttgcgcc gtcagggtgt ccgacgggcg gatagagaaa aaaggcttgc
     1501 atataatgta aacccccttt aaaattgcgc gtttacagaa tttatttttc tttcaggaga
     1561 ttccaatatg gcaaacagcg cacaagcacg caaacgtgcc cgccagtccg tcaaacaacg
     1621 cgcccacaac gctagcctgc gtaccgcatt ccgcaccgca gtgaaaaaag tattgaaagc
     1681 agtcgaagca ggcgataaag ctgccgcaca agcggtttac caagagtccg tcaaagtcat
     1741 cgaccgcatc gccgacaaag gcgtgtttca taaaaacaaa gcggctcgcc acaaaagccg
     1801 tctgtctgca aaagtaaaag cactggcttg atttttgcaa aaccgccaag gcggttgata
     1861 cgcgataagc ggaaaaccct gaagcccgac ggtttcgggg ttttctgtat ttcgggggta
     1921 aagttcgaaa tggcggaaag ggtgcggttt tttatccgaa tccgctataa aatgccgttt
     1981 gaaaaccaat atgccgacaa tgggggcgga gatgaataca cgaaatatgc gctatattct
     2041 tttgacagga ctgttgccga cggcatccgc ttttggagag accgcgctgc aatgcgccgc
     2101 tttgacggac aatgttacgc gtttggcgtg ttacgacagg atttttgcgg cacagcttcc
     2161 gtcttcggca gggcaggaag ggcaggagtc gaaagccgta ctcaatctga cggaaaccgt
     2221 ccgcagcagc ttggataagg gcgaggcggt cattgttgtt gaaaaaggcg gggatgcgct
     2281 tcctgccgac agtgcgggcg aaaccgccga tatctatacg cctttgagcc tgatgtacga
     2341 cttggacaaa aacgatttgc gcgggctgtt gggcgtacgc gaacacaatc cgatgtacct
     2401 tatgccgttt tggtataaca attcgcccaa ctatgccccg agttcgccga cgcgcggtac
     2461 gactgtacag gaaaaattcg gacagcagaa acgtgcggaa accaaattgc aggtttcgtt
     2521 caaaagcaaa attgccgaaa atttgtttaa aacccgcgcg gatctgtggt tcggctacac
     2581 ccaaagatcc gattggcaga tttacaacca aggcaggaaa tccgcgccgt tccgcaatac
     2641 ggattacaaa cctgaaattt tcctgaccca gcctgtgaag gcggatttgc cgttcggcgg
     2701 caggctgcgt atgctcggtg cgggttttgt ccaccagtcc aacggacaga gccgtcccga
     2761 atcgcgttcg tggaacagga tttatgccat ggcaggcatg gaatggggca aattgacggt
     2821 gattccgcgc gtgtgggtgc gtgcgttcga tcagagcggc gataaaaacg acaatcccga
     2881 tattgccgac tatatggggt atggcgacgt gaagctgcag taccgcctga acgacaggca
     2941 gaatgtgtat tccgtattgc gctacaaccc caaaacgggc tacggcgcga ttgaagccgc
     3001 ctacacgttt ccgattaagg gcaaactcaa aggcgtggta cgcggattcc acggttacgg
     3061 cgagagcctg atcgactaca accacaagca gaacggtatc ggtatcgggt tgatgttcaa
     3121 cgactgggac ggcatctgaa taccgtttca aacggcatat caagttgccg tgccgtctga
     3181 agccgccggc ggtttggcgg ggcgggataa aaaattcgtt tgaatccggg caaacgcgta
     3241 taatgtgcgg tttgtacgga ctttgtcgga atcaagcaag atgacggaac ctgcggccga
     3301 aggcggcaaa gctgccaagg cgttaaaaaa atatctgatt acaggcattt tggtctggct
     3361 gccgattgcg gtaacggttt gggtggtttc ctatatcgtt tccgcgtccg accagcttgt
     3421 caacctgctg ccgaagcaat ggcggccgca atatgttttg gggtttaata tccccgggct
     3481 cggcgttatt gttgccattg ccgtattgtt tgtaaccggg ttatttgccg caaacgtgtt
     3541 gggtcggcag attcttgccg cgtgggacag cctgttgggt cggattccgg ttgtcaaatc
     3601 catctattcg agtgtgaaaa aagtatccga atcgctgctg tccgacagca gccgttcgtt
     3661 taaaacgccg gtactcgtgc cgtttcccca atcgggtatt tggacaatcg cattcgtgtc
     3721 cggtcaggtg tcgaatgcgg ttaaggccgc attgccgcag gatggcgatt atctttccgt
     3781 gtatgtcccg accacgccca acccgaccgg cggttactat attatggtaa agaaaagcga
     3841 tgtgcgcgaa ctcgatatga gcgtggacga agcgttgaaa tatgtgattt cgctgggtat
     3901 ggtcatccct gacgacctgc ccgtcaaaac attggcagga cctatgccgc ctgaaaaggc
     3961 ggagttgccc gaacaacaat aaagccgccg ttcagacggc attttctgtt ttcagtttaa
     4021 atcaataaaa ggtgatttta tgcgtaccaa ctattgcggc ctgatcagcg agcaatactt
     4081 aggccaaacc gttaccgtca aaggctgggt acaccgccgg cgcgaccacg gcggtgtgat
     4141 ttttatcgac ctgcgcgacc gcgaaggcat cgtccaagtc gtgatcgatc ccgacacgcc
     4201 cgaagcgttt gccgctgccg attccgcccg taacgaatac gttttgggca ttgccggccg
     4261 cgtacgcaac cgtcccgaag gcacgaccaa cgacaaaatg atttccggca aaatcgagat
     4321 tcttgccaaa gaaatcgaag tgttgaacgc cgccgctacg ccgccgttcc aaatcgacga
     4381 cgaaaacatc agcgaaaacg tgcgcctgac caaccgcgtt atcgacctgc gccgccctgt
     4441 gatgcagcgc aacctgcgcc tgcgttacca agtcgctacg ggcgttcgcc gttatctgga
     4501 cgcgcaaggc ttcatcgaca tcgaaacgcc gatgctgacc cgctccactc cggaaggcgc
     4561 gcgcgactac ctcgtaccga gccgcgttca tccgggcgag tttttcgcgc tgccgcaatc
     4621 gccgcaatta ttcaaacaac tgttgatggt ggcgggtttc gaccgttact accaaatcac
     4681 caagtgcttc cgtgacgaag acttgcgcgc cgaccgccaa cctgaattca ctcaaatcga
     4741 cttggaaacc tcgttcctga acgaggatga aatcatggac atcactgaag gcatggccaa
     4801 acaagtcttc aaagatgctt tgaacgtaga tttgggcgac ttcccacgca tgccttattc
     4861 tgaagccatg ttctactacg gctctgacaa accggacatg cgcatcaact taaaattcac
     4921 cgagttgacc gacctgatga aaacggaaga attcaaagtc ttccgtggcg cagccgacat
     4981 gaaaggcggc cgcgtagtcg ctctgcgcgt gccgaacggc gccaaattca gccgcaaaga
     5041 aatcgacgaa tacactaaat ttgtcggcat ctacggcgcg aaaggtctgg catacatcaa
     5101 agtaaacgat gcgggcaacc tctccaacgg cgaagacagc ggcctgcaat ctccaatcgt
     5161 aaaattcttg tctgaaaacg ccctgaaaga aatcatcgag cgtaccgccg cgcaaaacgg
     5221 cgacatcatc ttcttcggtg cggacaaagc caaagtcgtg aacgaagccg tcggcgcatt
     5281 gcgtatcaaa gtcggcttgg aacacggcaa agacaacggc tacttcacag acgaatggaa
     5341 acctttgtgg gttgtcgatt tcccgatgtt cgaatacgac gaagaagccg accgctacgt
     5401 tgccgtacac catccgttta ccgcgcctaa agaaggtcat gaagacctga tggtttccga
     5461 tcctgcaaac tgcctggccc gtgcctacga tatggtattg aacggctggg aaatcggcgg
     5521 aggctctatc cgtatccacc gtgcagacgt acaagagaaa gtgtttgccg cgctgaaaat
     5581 cagccctgaa gagcaacaag agaaattcgg cttcctcttg gacaacctga aattcggtgc
     5641 gcctcctcac ggcggccttg cattcggcct cgaccgtctg gtaacgctga tgaccggtgc
     5701 cgaatccatc cgcgacgtga ttgccttccc gaaaacacaa cgcgcccaat gcctgctgac
     5761 cgatgcgccc aacagcgtgg acgataagca gttgcgtgaa ttgagcctgc gtctgcgtca
     5821 aaaagcagtt gaaacaaaag aagcataagg aaaacagagc cgtttgacgg ctctgttttt
     5881 tcagacggca tttacgcttc ttgacttccc tctaattcaa acctaatttt gctgtgtttt
     5941 aatggcggta ttgaaaaaca catcttgttc aatcaaccga taaaaaaagg actgaaaatg
     6001 aaaaaactgt tattggccgc cgttgtttcc ctaaatgccg caaccgcatt tgccggcgac
     6061 tctgccgagc gtcagattta cggcgatccc cattttgaac aaaaccgcac aaaagccgtg
     6121 aaaatgttgg aacagcgcgg ttatcaggtt tacgatgtcg atgccgacga ccactggggc
     6181 aaacctgttt tggaagtgga agcctataaa gacggccgcg aatacgacat cgtgttgtct
     6241 taccccgacc tgaaaatcat caaagagcag ctcgatcgct gactcctttg atggaaagat
     6301 gaaccaaaat gccgtctgaa gcgttcagac ggcattttgc ctgttcctca tcaggtatga
     6361 ggcaggcttt tcttattaaa aaaatgacat ttcacgctga tttgttataa tcattccttt
     6421 tcaacacgac agacggagca ggtttattat gcctatcctt accatccgtg aagtgtgcaa
     6481 cattaatcat tggagcatag gttattatga tgttgacgat tccggcgaaa tcatcgtccg
     6541 ccccaatccc tcgcaacaca atcaaactgt ttcactgcaa aaactgactg aagccgtgca
     6601 acaaaaacat caggcgcgcc tgcctgtttt gttttgtttt ccgcaaatcc tcgaacaccg
     6661 cctccgcgac attaaccgcg cctttcagac ggcacgggaa gagtgcggct ataagggcgg
     6721 ttattgtttg gtttacccta tcaaggtcaa ccaacaccgc cgcgtcatcg agtcgcttat
     6781 gtcaagcgga caaccgcatg gtttggaagc cggttctaaa gccgaactga tggcggtttt
     6841 ggcacacgcc ggcacccggc aaacattaat cgtctgcaac ggctataaag accgtgaata
     6901 tatccgtttc gccttgatgg gcgaaaaact ggggcatcag gtttatttgg tgattgagaa
     6961 gctgtccgaa atacaaatgg tattggaaga ggcggaaaaa ctcggcatca agccccgttt
     7021 gggtgtgcgc gccagactgg cttcccaagg ttcgggaaaa tggcagtctt cgggtgggga
     7081 aaaatcaaaa ttcggcttgt cggcttccca agttttgcaa ctggtcgata ttttgaaaca
     7141 aaaaaacagg ctggactgcc tgcagctttt gcatttccat ttgggctcgc agcttgggaa
     7201 catccgtgat gttgccacag gtgtacacga atcggctcgg ttttatgttg agttgcacaa
     7261 actgggggta aatatccgct gttttgatgt aggtggcggg cttggcgtgg attacgaagg
     7321 aaaccgcaca caatcggatt gttccgttaa ttacagcctg aacgaatatg ccgccacagt
     7381 cgtatggggc atcagtcagg cttgtctcga acacgggctg ccgcatccga caatcattac
     7441 cgagagcggg cgcggcatta ccgcacatca cgccgttttg gttgctaatg ttataggcgt
     7501 tgaacgttac aaaccgcgtc ggctggatgc gccatcgccc gaagcaccgc gtgtgttgca
     7561 cagtatgtgg gaaacttgga cggatatttc cgcctcgcgg gaaaaacgtt ccttacgcag
     7621 ctggatacac gaagggcagt ttgatcttgc cgatgtgcat aatcagtata atgtcgggct
     7681 gttgagtttg gcgcaacgtg cgtgggcgga gcaactgtat ttaaatatct gtcatgaagt
     7741 cggcgaattg tttaatgaaa aacaccggtc tcaccgaacc attattgacg aattgcaaga
     7801 acgttttgcc gataagctgt atgtcaattt ctcactcttc caatctttgc ccgatgcttg
     7861 gggcatagat caacttttcc ctgtttgtcc tattaccggt ttgaatgaac cgattgcgcg
     7921 ccgcgccgtg ttgttggaca ttacctgcga ttcagacggt acgattgacc actacatcga
     7981 cggcgacggc atcgccggta cgatgcctat gcctgattat cccgaagaag agccgccgtt
     8041 tttaggcttt tttatggtgg gagcatatca ggaaatactc ggcaatatgc acaatctttt
     8101 cggcgacact gccactgctg atgttgttgt aagggaagac ggacaattta ccgtcatcga
     8161 ttacgatgaa ggaaacaccg ttgccgatat gctcgaatac gtttatcaag atccgaaaga
     8221 gctgatgaaa cgctatcgcg aacaaatcga acattcagac cttcctgcct cgcaggctat
     8281 gtctttctta aaagaactcg aagcggggct taatggttat acctatttgg aagacgaata
     8341 gacgcatcaa ggtatcggat atgtcgtctg aagcccgatt ttcttactca aacaccaatc
     8401 atcacgaccg attgaaacca attacaagga atcattacga tgcaatacag cacactggca
     8461 ggacaaaccg acaactccct cgtttccaat aatttcgggt ttttgcgcct gccgcttaat
     8521 tttatgccgt atgaaagcca tgccgattgg gttattaccg gcgtgcctta tgatatggcg
     8581 gtttcagggc gttccggcgc gcgtttcggt cctgaagcca tccggcgcgc ctccgtcaac
     8641 ctcgcttggg agcaccgcag gtttccgtgg acatttgatg tgcgcgaacg cctgaacatt
     8701 attgattgcg gcgacttggt tttttctttt ggcgacagca gggattttgt cgaaaaaatg
     8761 gaagcgcacg ccggcaaatt actttctttc ggcaaacgct gtttgagttt gggcggcgac
     8821 catttcatta ccctcccgtt gttgcgcgcc cacgcccgct atttcggcaa actcgcactg
     8881 attcattttg acgcgcacac cgacacctac gacaacggca gcgaatacga ccacggcacg
     8941 atgttttata ccgcccccaa ggaaggcctc atcgacccgt cccgttccgt acaaatcggc
     9001 atacgcaccg aacacagtaa aaaattgcct tttactgtgt tgtccgcccc caaagtcaat
     9061 gaagacagtg ttgaagagac cgtccgtaaa atcaaagaaa ccgtcggcaa tatgcccgtt
     9121 tacctgactt tcgacataga ctgtctcgac ccgtcgttcg cccccgggac cggtacgccc
     9181 gtatgcggcg gcttgagcag cgacagggca ttaaaaatcc tacgtgggct gacggatctc
     9241 gacatcgtcg gtatggatgt tgtagaagtt gccccctctt acgaccaatc cgacattacc
     9301 gctttggccg gcgccacaat tgccttggaa atgctttacc ttcaaggtgc gaaaaaggac
     9361 tgaacgtccg gcatcccccg ggttttcgcc gtgccgttca aacggcgtat tcagtctaat
     9421 gaaaattcaa atactgaaac aaaagttgcc cggagccgca tatcggaaag acggtgaaat
     9481 atcgtaatat aacttataaa acaattagtt aaatattatt tttccgattt ttcgggacgg
     9541 tcttttttac ggaggtcaat atgatgaaat tgggtttcaa accgataccc ctcgccattg
     9601 ccgcagtatt gtgcgccctg gttttggcac tgcccgtacc cgacggggtc aagcctcagg
     9661 cttggacgct gctggctatg tttgtcggtg tgattgccgc cattatcggc aaggttatgc
     9721 cgttgggcgc gctgtcgatt attgccgtcg ggttggtcgc agtaaccggc gtaaccgccg
     9781 acaaaccggg cgcggcgatg agcgatgcgt tgagtgcgtt cgccaatccg ttgatttggc
     9841 tgattgccat cgcagttatg atttcgcgcg gtttgctcaa aacagggctg gggatgcgta
     9901 tcggatattt gtttatcgcc gtttttggaa gaaaaacgct gggcatcggt tacagtctcg
     9961 ctctttccga actgctgctg gctcccgtta ccccttccaa taccgcgcgc ggcggcggca
    10021 ttatacatcc gattatgcag tcgattgccg gcagttacgg ctccaatccc gcaaaaggca
    10081 cagaaggcaa gatgggtaaa tatttggctt tggtcaatta tcattccaat cccatttcgt
    10141 cggctatgtt tattactgca actgccccca accctttaat cgtcaacttg attgccgaaa
    10201 atttaggcag tagtttccgt ctttcttggg gggcgtgggc gtgggcaatg gctgttcccg
    10261 gcgttatcgc ctttttcgtt atgcctttga ttttatattt tttgtatccg cctgaaatta
    10321 aagaaacgcc caatgctgtt caatttgcca aagaccgtct gagggagatg ggtaaaatgt
    10381 cggcagacga aatcattatg gcggtcattt tcggtatctt gctgctgttg tgggcagatg
    10441 ttcccgccct tattaccggc aatcacgctt ttagtatcaa cgccaccgcc accgcattta
    10501 tcggattaag cctgcttttg ctttccggtg tattgacttg ggacgatgtt ttgaaagaaa
    10561 aaagcgcgtg ggatacgatt atttggtttg gcgcattgat tatgatggcc gcatttttaa
    10621 ataaactcgg actgattaaa tggttctccg gagtgttggc ggaaagtgtc ggcggtttgg
    10681 gcgttagcgg cacggctgcg ggcgtaatcc tcgtgcttgc ttatatgtat gcgcattata
    10741 tgtttgccag tactactgca catattaccg ctatgttcgg cgcatttctc gctgctgccg
    10801 tttcactgaa tgccccggcg atgccgaccg cgctgatgat ggcggccgca tccaacatta
    10861 tgatgaccct cactcattat gcgaccggta cttcacctgt gattttcggc tcgggctaca
    10921 ccacaatggg agaatggtgg aaggcgggtt ttgtcatgag cgtagtcaat tttctgattt
    10981 tttccgttat cggcagcatt tggtggaaag ttctgggata ttggtaaagg gaaaaataaa
    11041 tttccaatct gtgtttattt gattgggcga ctattatcgt gaaatatgcc gtctaaagcc
    11101 ttcagacggc atatttgtgc gcttggatgt tgcagaaagc ggcagggcgg cggtgtagga
    11161 aaagccaaac aaaaaccaaa ccgcctgtca acttctgata aacataagta ttaaataatc
    11221 agaaggttat tcaattacct aaacgcaaat tcccctgccg tatcacatct attgaaaata
    11281 atacatcaac cggctcggaa gcagcctgat caggtgattc tacttgcggc gatgaatcgg
    11341 cagccggttc ggtataggca gtcggcgtgc cgtcggattg ggtatttaaa tgcgcttgtg
    11401 cctcgggcgt gtgtacatca ggcacttcgg gcgtgcgtgt ctcggatact tcggcagagt
    11461 tggtttcctc agtttgttca atgacttcag cttggctgta tgaggaagaa ccctgtatcc
    11521 acgccagcga tttgagcggc atcttcatct tgccgttttt gccgcaggtc agacagacgg
    11581 ccgatccggt gcggtctttg agtacggcat cgactttctc gggcgcgatg gttttgccct
    11641 gtgtttttgc ccattgcccg atgctttttt tcagggaggc ggtgtcaagg ttgttcttac
    11701 cgtaagggtc gcggaaggcg gcaagccaca ggttgcgatc cgcatcttca ttgaggcccg
    11761 ccatttgata gatgacggag gcgggcgagc cggtggcgat ggttttggca aactcgagcg
    11821 cgtcgggcga tttcatgcgg acgcagccgt gactccggat gcccgggacg ctggccggtg
    11881 cattggttcc gtgtatgccc aaaccgagtt tcgggtctcc taagcggaca aaaaccggcc
    11941 ccaaagggtt gtccgggccg gcggctatgg tttttacgcc gtcgccgcgt tctttctgta
    12001 tggattttgg gatgtgccaa acagggttat aggctttcgc cccgatttta tgttcgccta
    12061 gattggtttg tgtcatcgcc cgacctactg caacgggata aaccttggtc agtttgccgt
    12121 cggtgtagag gaacaggcgt tgctgaggga tgttaatgaa gacatgttga ccttgtgcga
    12181 cgggggagac atcgggaatg acggtgtttg cgtatgaaaa accgcttatc aatagtgcgg
    12241 cagtgcggca gattgtttta ttcatatcaa aatatggtgt gtgtccgata ggttttcggc
    12301 aaatcatacc tgaaaccgta ccaatttgtg cgaaaatatg cgcttcggta cagtgcggac
    12361 ggattgggta atggcaacgg aaacaaatat cgcggaaatt tccgccttgg attatgaagg
    12421 caggggtgtg gcaaaggtcg gcggcaaaac gattttcatt aaaggggcat tacttgattg
    12481 tttgatgctg ggttggttca ggctttaact caggaatatt tacatcataa tgaaagtttt
    12541 tagacaacag cttgaacaac tcggcgcgca aaatcaatat cgttcgattc cggatttgat
    12601 tcatcaaggg cggtatatta cgcgggaaaa ccgcaaaatg ctgaatatgt cgtctaatga
    12661 ttatttgggg ttggcatcag atgaaaactt acgccggtct tttttgcagc aatacggcgg
    12721 taattttccc tcttttacca gttcttcatc acgtttatta acgggcaact ttcctattta
    12781 taccgatttg gaagagcttg tcgcacaacg tttccaacgg gaaagcgcgt tattgttcaa
    12841 cagcggctat cacgccaatc tcggtatttt gcctgctttg acgacgacga aaagtttgat
    12901 tttggcagat aaattcgttc acgccagtat gattgacggc atccgtttga gccggtgtgc
    12961 gtttttccgt tatcgtcata atgattatga acatttgaaa aatctgcttg aaaaaaacgt
    13021 cggaaaattt gaccgcactt ttatcgttac cgaatctgtt ttcagtatgg acggcgatgt
    13081 ggcggatttg aaacagcttg tccaattaaa aaaacagttt cccaatactt atctttatgt
    13141 ggatgaagcc cacgcaatcg gtgtttatgg gcaaaacgga ttggggattg ccgaacggga
    13201 taatctgatt gccgagattg atttattggt cggcactttc ggtaaagcct tagcctcggt
    13261 gggggcgtat gccgtctgca accaagtatt gaaagaatgt ttgattaatc aaatgcgccc
    13321 attgattttt tcaaccgcat tgccgccgtt taatgtggct tggacttatt ttatttttga
    13381 acgattgccg caattctcaa aagaaagaag tcatcttgag cagttaagcg catttttacg
    13441 gcgggaagtg gcgcatcgga cgcaaataat gccgagcgaa acctgtatcg tcccctatat
    13501 tttaggcggg aatgaagcca cccttgccaa agcggaatat ctgcaaggtc agggttatta
    13561 ttgcctgccc atcagaccgc cgacagtacc aaaaaacaca tccagaatcc gcctgtcttt
    13621 aactgccgat atgacaacgg atgaagttcg gcagtttgcg gcgtgcctgt aaggatatga
    13681 tatgaaaaca aaattttaca atcatcaagg cgaacattta atcctgtatt ttgcaggttg
    13741 gggaatgccg cccgatgctg taaatcattt gattttgccg gaaaatcacg atttattgat
    13801 ttgctatgat tatcaagatt taaatttgga ttttgatttt tccgcctatc ggcacatccg
    13861 tttggtggcg tggtcaatgg gcgtttgggc ggcagagagg gcattgcaag gaataagatt
    13921 aaaatccgca acggcagtga atggcacagg tttgccttgc gatgataatt tcggtatccc
    13981 ttgcgccgtt tttaaaggca cattggagaa tctcacagaa aatactcgtt caaagtttga
    14041 acgtagaatc tgtggcgata aagcatcttt tgaagattac caacaatttc ccgcccgtcc
    14101 gtttgacgaa attcatcaag aacttaccgc actttttgcg atgatcgggc aagataggcg
    14161 tacagatctt atccgctgga caaacgcctt gttcggatcg ggcgataaaa tttttattcc
    14221 tgccaatcag caccgatatt ggacaccgcg ttgcaccgtt caagaaacag acggcgggca
    14281 ttacctgttt tcaagattta cccattggtc ggcactatgg aatcactgac tgccataaat
    14341 aaaccgcgca ttcggcaggc tttccaaaaa gcattaagcg attatgacag gcacgcctta
    14401 atccaacaaa aaatgacgat taatctaatc gcgcatttgc aagattattt gccggatatg
    14461 ccattggaaa acgtgttgga attgggctgc ggctcaggaa tgttgagtgc cttgctgcaa
    14521 aaacagattt cagcgaatta ttggttattt aatgatttgt gcgatgtacg aagccgactg
    14581 gctgaaaaat tgcctcaatc ctttgatttt tattgcggcg atgcggaaaa ctttcctttt
    14641 caacgacaat ttgacttaat cgcaagcgca tctgccgtgc aatggtttca ccaacccgac
    14701 gcttttatcg cccattgcaa aacaggcttg aaaacaaacg gattattggc ggttgcgacc
    14761 tttggcaaag acaatttaaa agaagtccgc caaatcacaa atataggctt aaattacccg
    14821 actttatccc aatggcaggc ttggttagcc aaagattttg agcttttatg gtgtgaggat
    14881 tttaaggtaa tactagactt tgatacgccg ttagatgtac tcaaacacct taaatataca
    14941 ggcgttaccg ccacgaacca aaaaaattgg acaagaaaaa atctcaatgg atttattggc
    15001 gattacttgt cggcgttcgg tatgccgtcg ggcaaagtgc gcctgactta tcatccgtta
    15061 ttttttatcg cgcgccactc tgcggcgggc aggcaataag gcagcttatg ggcaaagtta
    15121 tttttatatc gggtattgat actgatgtgg gtaaaaggta atatggcgag ccttatgcag
    15181 aaggcatatt gtcaaacgtt aaattatggt atgatttaaa atttacaagt ctatttcagt
    15241 aaatcgttaa taataaaagc ggacaattta ataataaaag cggataattg gccgttgcag
    15301 gcggtcagct atccgctcgt tacggtatcg ggggtttcct ttggaaaact cattaattat
    15361 cggtctggca ctggcggtat tgctgatgct tttggttatg cgtgcgaagc agggtaagaa
    15421 aacaccgaag cgcaaatccc aaggtgtcgg caatacgcag gggcagccgc ccggaagcaa
    15481 tgattcggac tgggtcgatc agacttggtc aatcggtttc agacggcacg caactcgact
    15541 ggtcttggaa cgaaagtgcc gagaccgcat ccgccgccgt atccgcgcaa gaagtcgatc
    15601 cgcttacgga gtatcaggtt tataagcaat tcggttatca gggcaaggct gccgaatctt
    15661 tggctgccta tctggacggc attccggatg gtgaagcgaa acctgagaac cttatccgcg
    15721 agctgatcga tatcaatctc gaagtggggg atgtcgatgt tttggcagac aatctgcaaa
    15781 aatacggcag actgattcct ttcgaacttt tggcaaaata tatcgaacag gcattacagc
    15841 gagattcaaa ccatttgcgt atccgcgtct tggcggaaga aggtttggga tagggcactc
    15901 aggagattga aaaacgtgcg gaaggcggtt ctgcgacggc agcttccgca tcgcccccgc
    15961 cggatgtcgg cggtaaggct tatggagccg aagaaatcaa gcgcatcccg attgtgcggg
    16021 gcaaaaaaga cgtgtccgga atcagccaag agaaaatcgg tgcgattgcc ggcctggtcc
    16081 gcgccgatca aggtgcgaaa
//
LOCUS       DAXDSD010000052        15945 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXDSD010000052 DAXDSD010000000
VERSION     DAXDSD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15945
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.052"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..251
                     /locus_tag="ACFB2Q_001242"
     CDS             <1..251
                     /locus_tag="ACFB2Q_001242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918269.1"
                     /translation="ILKQIISKSDFFKIKFQKKETGKIRHKTLTYSKPSVLQRKYPKK
                     TICLPSVSNRYTAGTRYFSDGAAVVRTDKNVTAAERIA"
     gene            305..2689
                     /gene="ppsA"
                     /locus_tag="ACFB2Q_001243"
     CDS             305..2689
                     /gene="ppsA"
                     /locus_tag="ACFB2Q_001243"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC0918270.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2910..4169
                     /gene="rho"
                     /locus_tag="ACFB2Q_001244"
     CDS             2910..4169
                     /gene="rho"
                     /locus_tag="ACFB2Q_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC0918271.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIILLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            4783..6087
                     /locus_tag="ACFB2Q_001245"
     CDS             4783..6087
                     /locus_tag="ACFB2Q_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC0918272.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(6181..7476)
                     /locus_tag="ACFB2Q_001246"
     CDS             complement(6181..7476)
                     /locus_tag="ACFB2Q_001246"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC0918273.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(7603..8490)
                     /locus_tag="ACFB2Q_001247"
     CDS             complement(7603..8490)
                     /locus_tag="ACFB2Q_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC0918274.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(8490..9455)
                     /locus_tag="ACFB2Q_001248"
     CDS             complement(8490..9455)
                     /locus_tag="ACFB2Q_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC0918275.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISSA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(9471..10595)
                     /gene="potA"
                     /locus_tag="ACFB2Q_001249"
     CDS             complement(9471..10595)
                     /gene="potA"
                     /locus_tag="ACFB2Q_001249"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC0918276.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLNIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(10873..11142)
                     /gene="rpsO"
                     /locus_tag="ACFB2Q_001250"
     CDS             complement(10873..11142)
                     /gene="rpsO"
                     /locus_tag="ACFB2Q_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC0918277.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(11223..11357)
                     /locus_tag="ACFB2Q_001251"
     CDS             complement(11223..11357)
                     /locus_tag="ACFB2Q_001251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918278.1"
                     /translation="MWSDVKCRLKPEDVSVFRRHFADIAKYRAEQQKLCDNALFLFQE
                     "
     gene            complement(11347..12282)
                     /gene="secF"
                     /locus_tag="ACFB2Q_001252"
     CDS             complement(11347..12282)
                     /gene="secF"
                     /locus_tag="ACFB2Q_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC0918279.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVARGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(12286..14139)
                     /gene="secD"
                     /locus_tag="ACFB2Q_001253"
     CDS             complement(12286..14139)
                     /gene="secD"
                     /locus_tag="ACFB2Q_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC0918280.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(14351..14617)
                     /gene="yajC"
                     /locus_tag="ACFB2Q_001254"
     CDS             complement(14351..14617)
                     /gene="yajC"
                     /locus_tag="ACFB2Q_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC0918281.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            14748..15866
                     /locus_tag="ACFB2Q_001255"
     CDS             14748..15866
                     /locus_tag="ACFB2Q_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC0918282.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPACTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPGHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
BASE COUNT         4009 a         4349 c         3994 g         3593 t
ORIGIN      
        1 atattttaaa acaaattatc tcaaaatcag atttctttaa aataaaattc caaaaaaaag
       61 aaacaggaaa aatacggcat aaaacattaa cctattcaaa accatctgtt ttacaaagga
      121 aatacccgaa aaaaacaata tgcctgccat cggtttcaaa ccgctacaca gccggaaccc
      181 gctatttttc agacggcgca gcggttgtcc ggacagataa aaacgtgaca gcggcggaac
      241 gaatcgctta aaataagcaa gcggatttgt ttctttttta acatattttg gattggacac
      301 acaaatggcc gacaactacg taatctggtt tgaaaacctg cgtatgacag atgttgaacg
      361 cgtgggcggt aaaaacgcct cgctgggcga aatgatcagt caactgaccg aaaaaggcgt
      421 tcgcgtcccc ggtggctttg ccaccacagc cgatgcctac cgcgcatttc tcgcacacaa
      481 cggtctgaac gaacgcattt ccgccgctct ggcaaaattg gatgtcgaag acgtttccga
      541 actggcacgc gtcggcaaag aaatccgcca atggattttg gacacgccct tccccgaaca
      601 gctcgatgcc gaaatcgaag cggcatggaa caaaatggtt accgatgccg acggcgcgga
      661 catttccgtt gccgtacgct cttccgcaac cgccgaagac ctgcccgacg catcatttgc
      721 cggccaacag gaaaccttcc tgaatatcaa tggcttggat aacgttaaaa aagcgatgcg
      781 ccacgttttc gcctccctgt acaacgaccg cgccatttcc taccgcgtcc acaaaggctt
      841 cgaacacgac atcgtcgccc tttccgccgg cgttcaacgc atggtgcgtt ccgacagcgg
      901 cgcatcaggt gtgatgttca ccctcgacac cgaatccggc tacaaccaag tcgtctttgt
      961 tacctcctct tacggtctgg gcgaaaacgt cgtacaaggc gcggtcaacc cggacgaatt
     1021 ttatgtgttc aaacccacgc tcaaagcagg caaacccgcc atcctgcgca agaccatggg
     1081 ttcgaaacac atcaaaatga ttttcaccga caaagccgaa gccggcaaat cggtaaccaa
     1141 cgtcgaagtc cccgaagaag accgcaaccg cttctccatt accgacgaag aaatcaccga
     1201 gttggcacac tacgcgctga ccatcgaaaa acactacggc cgcccgatgg atatcgaatg
     1261 gggacgcgac ggcctggacg gcaaactcta catcctgcaa gcccgccccg aaaccgtcaa
     1321 atcccaagaa gaaggcagcc gcaacctgcg ccgctacgcc atcaacggcg aaaaaaccgt
     1381 cttgtgcgaa ggtcgtgcca tcggtcaaaa agtcggtcag ggcaaggtgc gcctgattaa
     1441 agatgcttcc gagatggatt ccgtcgaagc cggcgacgta ctggttaccg acatgaccga
     1501 tccggattgg gaacccgtga tgaaacgtgc ttccgccatc gtgaccaacc gcggcggacg
     1561 cacctgccac gccgccatca tcgcgcgcga actgggtatt cctgccgtcg tcggctgcgg
     1621 caatgcgacc gaactcttgg aaaacggtca ggaagttacc gtatcctgtg ccgaaggcga
     1681 taccggcttt atctatgccg gcctgctgga cgtacaaatt accgatgtcg ccttggacaa
     1741 tatgccaaaa gcacccgtca aagtcatgat gaacgtcggc aatcccgaac tcgcattcag
     1801 cttcgccaat ctgcccagcg aaggcatcgg cttggcgcgt atggaattta tcatcaaccg
     1861 ccaaatcggc atccacccga aagccttgtt ggaatttgac aaacaagacg acgaactgaa
     1921 agcggaaatt atccgccgca tcgccggtta cgcatcccct gtcgacttct acgtcgataa
     1981 aatcgccgaa ggcgtggcaa cgcttgccgc atcggtttat ccgcgtaaaa ccatcgtccg
     2041 tatgtccgac ttcaaatcca acgaatacgc caacctggtc ggcggcagcg tatacgaacc
     2101 gcatgaagaa aacccgatgt tgggcttccg tggtgcggcg cgttatgttg ccgagagctt
     2161 caaagactgt ttcgccttgg aatgcaaagc cttgaaacgc gttcgtgacg aaatgggtct
     2221 gaccaacgtc gaaatcatga ttccgttcgt ccgcactttg ggcgaagccg aagcagtggt
     2281 taaggcattg aaagaaaacg gcttggaacg cggcaaaaac ggcctgcgcc tgattatgat
     2341 gtgcgagctg ccgagcaacg cggtattggc ggaacaattc ctgcaatact tcgacggctt
     2401 ctccatcggc tcgaacgaca tgacccagct gaccctcggc ctcgaccgag acagcggctt
     2461 ggtgtccgaa tcgtttgacg aacgcaaccc tgccgtcaaa gtgatgctgc atcttgccat
     2521 ctccgcctgc cgcaagcaga acaaatatgt cggtatctgc ggtcaaggtc cgtccgacca
     2581 tccggacttt gccaaatggc tggttggaga aggcatcgaa agcgtgtctc tgaacccgga
     2641 taccgtcatc gaaacttggc tgtatttggc gaatgaattg aacaaataat caattcccat
     2701 acccccaagc ctgaaaagcg cgggggtatt ttttccaagc agctccgttc agacggcatt
     2761 tcctgtcgat gccccgtccg cgataatatt tgacacccac gcgccgactg cctacaattc
     2821 ccccctctcc gagcaaccgg caacagtcag cttcttcttt cagacggcat ccacccgtct
     2881 tttcctttct attctatata tcattgatta tgcacgtctc cgaattacaa accctgcaca
     2941 tttccaaact cttagaattg gcggaagaac acggcatcga aaacgccaac cgattccgca
     3001 aacaagacct cgtatttgcc atcgtccgcc agatgatgaa aaaaggcgag ggttttacct
     3061 gctccggcac gctcgaaatc ctgcccgacg gcttcggctt cctccgcagc gcggacacgt
     3121 cctatcttgc cggccccgac gacatctatg tctcgcccac tcaaatccgc cgcttcaacc
     3181 tgcatacggg cgacaccatc gaaggcagcg tgcgcgtccc caaagacaac gaacgctatt
     3241 ttgccttggt cagactggat tccatcaatg gcgaccaccc ggaagtatgc cgccataaaa
     3301 tcctgtttga aaacctgacc ccgctgtttc cgaccgaaca gttgaagctg gaacgcgact
     3361 taaagtccga agaaaacctg accggacgcg ccatcgacct gatttcgccc atcggcaaag
     3421 gtcagcgcgc cctcttggtt gccccgccca aaatcggtaa aaccgtgatg ctgcaaaaca
     3481 ttgcccacgc cgttaccgca aactatcctg aagtcgaact catcatcctc ttgattgacg
     3541 aacgccccga agaagtaacc gaaatgagcc gttccgtccg aggtgaagta gtctcctcca
     3601 cctttgacga accggcgcaa cgccacgtcc aagttgccga aatggtgctt gaaaaagcca
     3661 agcgtatggt ggaacacaaa aaagacgtgg tcatcctgct ggattcgatt acccgccttg
     3721 cccgcgccta caataccgtc gtccccgcct cgggcaaaat cctgaccggc ggtgtcgatg
     3781 ccaacgcgct gcaccgcccc aaacgtttct tcggtgcggc gcgcaacgtg gaagaaggcg
     3841 gttcgctgac cattatcgcc accgcattgg ttgaaaccgg cagccgtatg gacgatgtga
     3901 tttacgaaga attcaaaggt accggcaata tggaattgca cctcgaccgc cgtatggcgg
     3961 aaaaacgcct cttccccgcc atcaacatca acaaatccgg cacgcgccgc gaagagctgc
     4021 ttgtgccgaa cgaccagttg caacgtatgt ggttactgcg caagttcctg cacccgatgg
     4081 acgaaatcga agctaccgag ttcttaaacg gaaaaatcaa agcatctaaa aataatgatg
     4141 atttctttga actgatgcgc ggaaaataaa cgcgccgccc gcattaatgc gaaatgccgt
     4201 ctgaagcctg aaaatcgggt ttcagacgac attttcattc gcacggccgg cgcagctccc
     4261 cccccgctaa cggcgcaacc gccggcagtg tccgtgtccg cctgccgaag gcgcggcctt
     4321 tgcaaaaccg gctcgaacgc atccctaccg gcaatgaaaa ccgatgcggg aacttgattc
     4381 aaggttgcgc cgaaccgcgc ccatttttgt ataaatttta ttttttattt ccaattagaa
     4441 atttttaata tattaatcaa taaattaatt ttataaaata aaaatattgt caacaatctt
     4501 ttgacttatc gcccaaacct ctgtatattt tcctacagta aattgttgac aatccatacg
     4561 cccgcatatg cgccgcctaa ggataaatcc tcccgccgga caacgggggc aagggatagg
     4621 atgcgatatt tccatattca aacaagggat ttgtttcacg cacagggcgg cacatcggca
     4681 aaatccccgc gccggtccgg cagggcttgc gcccatcccg gacaagcccc gaccccgcct
     4741 ttccgaaaga cgggcccaac cattaaggaa actttaatca aaatgaaaaa acacatatgg
     4801 gcggcatctt tgctgccggc atccctatcg gcagaacctt taaactggtg gaaaccttat
     4861 tccgccgtca attcgggcga taccgcctgg gtgatgactg cggctgcctt ggtactgttg
     4921 atgacgcttc ccgggctggc tttattctac ggcggtatgg tgcggaaaaa aaacctgctc
     4981 tcgacgatga tgcacagctt ttccatcgcg acattggtgg gcatcctttg ggtcgccgtc
     5041 ggctattctt tggcgttcac gccgggaaat gcctttatcg gcggtttggg gcgcgtattt
     5101 ttaagcggga tgcagataga cgctgccgca cggatgctga ccgtgtcgcc caatgcgccg
     5161 actgttcccg aaccggtatt tatgtttttt cagatgacgt ttgccattat ttcgactgcc
     5221 attattaccg gcgcgtttgc cgaacgaatg aaatattcgg caatgatgct gttttcgggc
     5281 atatggtttt tattggttta tgtgccgggc gcgcattggg tgtggggcgg cggctttatg
     5341 agcaagggcg gcgtattgga ttatgccggc ggtacggtgg tgcacatcaa tgccggtatc
     5401 gcgggactcg tcgccgcctt ggttttgggc aggcgcatag gctacgggcg cgaggcgatg
     5461 cctccgcaca atatggcgat gacgctgatt ggcgcggcaa tgttgtggtt cggctggttc
     5521 ggctttaacg ctggttcggc gcttgcggca gacgcggcgg cggggatggc gatggcggta
     5581 acgcaggtgt cggccgtatt cggcgcggca ggctggcttg cctgcgagaa aatagcggga
     5641 cacaaacctt ctgctttggg gctggcttcc ggcgcagttt ccgggctggt cggcatcacc
     5701 cctgccgccg gctttaccgg tccgtcgggc gcggccgcca tcggtatatt gactgccgcc
     5761 gcgtgctttg tgtccgtcac cgtcgtcaaa cacaaattgc gttacgatga ttctttggac
     5821 gctttcggca tacacggatt cggcgggctg gtaggcggaa tattgaccgg catctttttc
     5881 gacaaccgca ttttcggtgg ggatgcggca gtttggcagc agttgtggat acaggtaaaa
     5941 gacgggttca ttatggcggc atacagcggg ctgatgagtt gggcgatttt gaaggctgtg
     6001 gggaaaatct gcggcggtct gcgcgtcggc aaggatgtcg aacgcgaagg tttggatctg
     6061 aatatccacg gcgaacacgt ggaataagga cggctatgcc gtctgaagcc tgaaaatcgg
     6121 gtttcagacg gcatttttca cgtttgccgc cgatggggat aaacatactg cctgcgacgc
     6181 ttaacggctg tctttccact gataatattt cgagccgagg aaagaaccga gtttgcgcaa
     6241 aaacggttgc aggataatat tcggctggcg caactgttca aacggtttca gacggcattc
     6301 gtcccctaaa attgcttcgg caaccgccag gcctgcaatg cccgttatcg ccatcccgtg
     6361 cccggaataa ccttgcgtat aaaaaacatt cgaggtcaaa cgtccgaaat gcgggacaag
     6421 gttggcggta atatcgcact ccccgcccca cgaatattcg attctaacat cggcaagctg
     6481 cggaaaaact ttaagcatat cttggcggac aagctcggtc atacgcgcag gattgtcgat
     6541 aaattcgtta tccttaccgc cgaaaagcag cctgccgtcc gcgctgaggc ggtaataatc
     6601 caaaatatgg cggttgtcgc atactgccat attgttgcgg ataagccctt ttgtgcgcgc
     6661 gcccaagggt tcggtggcaa taataaaggt gctgacggca atcgccttgc gttccaaagg
     6721 ccggaatatc gggttcaaac cgacataagt attgacggca tagaccacat ttttacactc
     6781 gacgctgcct tcgggcgtgt aaaccagcca accgttttga tacggttcga tgcgcgtcat
     6841 cggggattgc tcgaaaatct gcgcgccggc ttcggcagcg gcgctggcaa cacccaacgt
     6901 gtaattgagc ggatgaagat gcccggacaa gggatcgaac tgtgcgcctt ggtacatatc
     6961 gctgtcaagc tgctgtttca actcggcttt atcccaaagt tgataatgac tcgcaccgta
     7021 atgccgttgg gcgtgttcat gccactgctg caactcttcc caatgctgcg gacggacggc
     7081 aaccgtggca taaccgcgct gccaatcgca atcgatggca tgtttgcgga cgcgttcgtc
     7141 caccagttcg accgcctgca aagactgttg ccaaaaccat tgcgcctgct ccaagccgac
     7201 ctgtttttca atttccccca taccgcaggc gtagtcgctg ataacctgcc cgccactcct
     7261 gccggacgcg ccgaagccga tacgtgcggc ttccaaaacg acggcttcat gtccgtgttc
     7321 cgccagcggc aatgcggtac acaaaccgct caaaccgccg ccgataatgc aggtttcggc
     7381 tttcagacgg cattggagtt tcggataaac agtatgcgga ttaaccgaac taaaataata
     7441 agaaggcaga tattcttgaa aatcagggcg aatcattgtg tttgctttat cgggtatatt
     7501 ttcggacgga atgatacagg ctgtcgggcc atatcgtcca aacagaaaat cggttgaaaa
     7561 aaaacaggct gacccagtca tgcggtcagc ctgccttatt aattaatttg ctttctcggc
     7621 agccaatttt tcttggcggt aggcttctgc cgcttctcgg ttgcgcttgg ttgcctgcct
     7681 catcatccaa taattgacga tgatgaccaa tgttccgatg atgccgatca ggatggttgc
     7741 caagacattc atctgaggat cgagacccaa cttgatcttg gagaaaataa cctgcggcaa
     7801 tgtggatgaa ccggggccgg agaggaatga ggtaatcacc aaatcatcca aagacaaggt
     7861 aatgccgagt aggaagcctg aagcaatggc aggggcaatc aaaggcaaag tgatgacaaa
     7921 aaagattttc agcggacgcg cgcccaagtc cattgcggct tcttcgagcg actggtcaag
     7981 ctcgaccagc cgcgaacgga tgacaacggt aatgtatgcc atgcacagcg tcgtatgtcc
     8041 gaggaagatg gtgaaaaagc cgcgatcgaa gtagagatgt tgcaaccatt cgctgccctg
     8101 caaaaatatc tgcacctgaa taatcagcag cagcatagac agaccggtaa tcacgtcggg
     8161 catgaccatg ggcgcggaaa tcataccggc aaacagcgtg ctgccgcgaa agcgtttgat
     8221 acgcgccatc gcatagcccg ccagcgtgcc caaaacgacg gcggcaagcg aagacacaac
     8281 ggcaatccgc agcgacagcc aagcggcttc caaaatggtg tcgttttcca gcaatgcgcc
     8341 gtaccacttg gtcgaaaagc cgccccaaac ggtaacaagt ttggattcgt tgaacgaata
     8401 gatgaccaaa acaaccagcg ggatatacag aaacgccagc gatagtgcca acatcagttt
     8461 caagaaccaa gataatttgg atttctgcat tatttggctc cttcttccaa ttcgcggttt
     8521 tcatagtgct ggaacagggc aatcggtaca accagtagcg cgaccatcac gacggcgacg
     8581 gcagaagcca acggccagtt gttttgatca aagaatgcct gccacaatac tttaccgatc
     8641 atcaggtttt ccgaaccgcc gaccagctcg ggaatgacga actcaccgac agcggggacg
     8701 aaaaccagca tcgaacctgc aataatgccg gttttcgaca aaggcagggt aatcgtcaag
     8761 aacgatttga ccggtcccgc gcccaagtcg gaagccgctt cgagcaggcg gctgtccagt
     8821 ttcaccagct gcgtgtaaag cggcagaatc ataaacggca gataggcata aaccattacc
     8881 aaatttaacg aaaacgcatt atagaacaaa tctaatggtt ctctgataat acccatctta
     8941 atcaataagt tgtttatgat gccgttatgc ccgagcaaac ccatccacgc atagacacgc
     9001 agcaggaacg atgtccaaaa aggtaacatg atggcaagca gcaaaccatt gcggacggaa
     9061 ggattggcac gggaaatcgc ataggcggtc gggtaaccga ccaacaggca aatcaccgtc
     9121 gtcgtcagcg cagtcttaat tgaagaccaa taagtcatca gatagatatt actgttttca
     9181 ctgtcgccga aggggttaag ggtatgccaa aaattttgga aaatatcggc atagttttga
     9241 tagctgacgg cgatgttcag gcgacccaag tcctcatcta tcgtcgttaa aggagtaaac
     9301 ggtggaatgg cgatttcttg ttcggcagag ctgattttca gcacgatggc gaacggaatc
     9361 agaaacagca ccaaaagcca aatatacggt acggcaatca ccgcacgctg ccccggacgg
     9421 cggaacagtt tgtttttcag tttattaagg ttcatctcac tccccttaaa tcaacggaac
     9481 aacggagtcg gttggttttc cggccagctg atatagacgg tttcatccca agtcggcggc
     9541 gtgatgttac gcacatacca gtaaggtgca ggaacttggc ttttgacgac gcgcccgttg
     9601 gcgagtttga tatggtatat ggcgaagctg cccaaatagg caatttcttt taccgtgcct
     9661 ttcgcccagt tgtagtcgcc caaatattcg ggtttttctt tatataaatc aatatcctct
     9721 ggtcgaatac taacccaaag atcctgctcg ctcgggccac ccaaaccatg gtcgatacgg
     9781 acgtggtttt ccaaaccttc gcattcgata acggcatagt cggcatgatt ttcaatcacc
     9841 acaccgtcaa agatgttggt ttcgccgata aactcggcag tgaagcggct gttgggatag
     9901 tcgtacacgt cgctgggcgt gccgacctgc tgcaactgac cgtcggacat aatggcgatg
     9961 cgggtcgcca tcgtcatcgc ctcttcttgg tcgtgcgtaa ccataatgca ggttacgccg
    10021 acttgttcca gcgtattgac caactcgagc tgggtttgct ggcgcaattt cttatccaac
    10081 gcccccaaag gttcgtcaag caacaggatt ttcggacgtt ttgccaaact gcgcgccaag
    10141 gcgatacgtt gctgctgacc accggacaat tggtgcggtt tgcgtttggc aaatttggtc
    10201 atctgaacca gacgcagcat ttcttcgacg cgcgcatcga tttcgccttt aggcattttg
    10261 tcctgtttca gaccgaaggc aatgttttgc tccaccgtca tatgcggaaa aagcgcgtaa
    10321 ctttggaaca tcatattgat ggggcggtca tagggtgcaa gtttggtaat atcttgacca
    10381 tcaaggatga tttttccctg attgggactt tccatacccg ccagcatacg cagcagcgta
    10441 gattttccgc tgccggaact gcccaaaagg gcgaagattt cgttttgata aatgttcaag
    10501 tcgatgttat cgacagcgta attgtcacca aactttttca ccaaaccttg gatttgcaga
    10561 taaggtttgg ctgaagacgc tgtggttgcg gtcataatgg caatactcca ataaaaagac
    10621 gagtaccggc aaaacggatt ttcgaatggg tgataaaaag ctgtttgatt gctggcggga
    10681 gtcgcaacgt ctgatgccgt ctgaaactct tgtgaagcgc acgggcagca tgaaataaaa
    10741 aggggttcta aagaaccccg cattatatta gtttataagg tttttagggt aataaatatg
    10801 ggtttgagct tgtcggaaag caacagaaaa gaaaacaccg cccatttttc tgggcggtgt
    10861 cggaaagcgc aattatttac gcagacccaa gcgggtaatc aacgcgcgat acgtatcggg
    10921 ctgggtacgg cgcaaatagg ccaacaggcg gcggcgttgg ctgaccattt tcaacaggcc
    10981 gcgacggctg tggtggtctt tggggttggc tttgaagtgg ggggtcagat cgttgatgcg
    11041 gaaagtcaac agggcaactt gtacttcaga agagccggtg tcgccttctt tacgttggaa
    11101 atctttaacg atttgtgctt tttgttctac ggtcagtgcc ataatggaaa aactccgaaa
    11161 aataaaaaga accccaaatg ggattcggac aagtttgcca agcctcgaca acggcaaact
    11221 ccctattcct gaaacaggaa cagcgcatta tcacacaatt tttgctgttc tgcacgatat
    11281 tttgcgatgt cggcgaaatg ccgtctgaaa acggagacat cttccggttt cagacggcat
    11341 ttgacatcag accacaattt cttccttctg tttcgcttcc ttggcgatat tgtcgcggct
    11401 caaaccgaac atcagcaaga gcgggctggc aaccaatacg gaagagtaga tgccgaacac
    11461 gataccgatg gtcagtgcca tagaaaagcc gtgcaaggct gcaccgccga acaccagcat
    11521 agaaacaacc atcgcctcgg tcgaaccgtg ggtaatgatg gtgcggctca tcgttgcggt
    11581 aatcgcgttg tcaatgactt ccggcacggt atgtccgcgc atcgccggct tgcggaaatt
    11641 ttcacggata cggtcgaaga cgacgacgga ttcgttcaca gaatagccca atacggcaag
    11701 gatacctgcc aagacggtca gcgaaaattc ccattggaag aaggcaaagc agccgagaat
    11761 aatcacgatg tcgtgcatat tggcgataat ggcagatacg gcaaaacgcc attcgaaacg
    11821 catcgacagg taaataatga tgccgataac gacaaaacct aaagccatca atccattatt
    11881 taccaattcc tcaccgactt gcgggccgat aaattcgact tggcgcaaag taacgtcggg
    11941 actgtctttt ttcagcaaat ccataacctg attggacaac tgtgcggaag taacaccttc
    12001 tttgttcggc aggcggatca tgatgtgttt gttcgtaccc aatgcctgaa cctgtacatc
    12061 acccattttc agcgtatcga ggcgttcgcg catcttattg acatccgcac cctgctgata
    12121 ttggacttcc attaccgtac cgccggtaaa ttcgacagag aaattcaggc ctctggcaac
    12181 caaaaagaat acggcggcaa taaaggtaac caacgaaatg aaggttgtca gtttgccgta
    12241 gctcataaac ggaatatcgc gtttgatttt aaagagttcc atagcttact ccttacctgc
    12301 cgcagtttcg gcttcaggtt tccataccgc accgatggaa atgttctgca atttgcggcg
    12361 gcggccgtac cacagattga ccaacgcacg gaatacgacg acggacgaat acatcgaagt
    12421 cagaataccc aagcagtgta cgacggcaaa acctcttacc ggtccggaac cgaataccaa
    12481 aagcgcgata ccggcaatca gcgaagtcag gttcgaatca acaatggtcg cccatgcgtg
    12541 ttggaaaccg agattgattg cctgctgcgg cggcacgccg gcacgcaatt cctctcggat
    12601 acgttcgtta atcaagacgt tggagtcgat tgccataccc aaagtcaacg ccagcgcggc
    12661 catacccggt aacgtcaacg ttgcctgcat ggcagacaaa ataccgatta gaaacagtat
    12721 gttggcactc aatgcaatag tagaaaagaa acccatcagg cggtaataaa ccaccatgaa
    12781 tgcagcaacg atggcaaaac cccataaagt cgaatggaag cctttttcga tgttctcctt
    12841 acccaaagac ggaccgatgg tacgttcttc gacaatctgc atcggtgcgg caagagaacc
    12901 ggcacgcaac agcaaagacg tatcgttggc ttcggctgtc gtcatgcttc cggaaatttc
    12961 cacgcgtccg ccggtaatgg cagtacggat aaccggtgcg gttacaacct cggattttcc
    13021 ttggtcgatc aaaaccatcg ccatgcgttt gccgacattt gcggcagtca gttcgccgaa
    13081 aatgctgccg cccgcgctgt ccaagctcag actgacggca ggtgcgccca tttggtcgaa
    13141 actcggttgc gcatcgttga tgttgtcgcc ggtcagctcg acctgtttgc tgatcagcag
    13201 agtttcggga tgctccccgc cgcttgaaag cagctcataa ccgctcggca cgttgccttc
    13261 caatgcctcg cgcaacttgg caggatcgtc ctccaccatg cggacttcca aagtcgcggt
    13321 acggccgatg atgtcttttg ccttggcagt atcttgcacg ccgggaagct gtacgacaat
    13381 gcggtccaaa cctgactgtt ggataatcgg ctctgccaca cccaattcgt ttacacggtt
    13441 atgcagtgta gtgatgtttt gcttcaccgc atctgatcgt actttattca ccgcctcttc
    13501 cgaaagcgcc aatacaatat tgctgccttc tgaatttaac gttgcctcag gaaacagctt
    13561 gagtaactga ggcagtgcct tttgcacatc gccggcatcc tgcaaaggga cggtcaggct
    13621 gttttcagcc tgacgcaccg tgccgctgcg gattttttca cggcgcagtt cgcggcggat
    13681 gtcgcccgaa taacgctcaa acgttttctg catcgcggct ttcatatcga cctgcatggt
    13741 gaaatgcacg ccgccgcgca ggtccaaacc caaaaacatc ggattggctt tgattttcgc
    13801 catccattcg gggctgtccg ccaacaggtt gagcgcggta atataccctt cgcccaaagt
    13861 gttttcgata acgtcgcgcg ctttaagctg cgtttctgtg tctttgaaac gcactttcag
    13921 tgaattatcc acaacaaaca tcccgtcggt ctgaatgccc gcgtttttca gcgcagcatc
    13981 cactttggat tgagtctgtt cgttgatgat gatggcttgt cggttggtcg atacctgcac
    14041 ggcgggcgtt tcgccgaata ggttgggcag cgaatacact gcggcaaccg caatcgtgaa
    14101 cacaatcagc agatatttcc ataaaggata acggttcatc attgttcctt aatggttgga
    14161 accccaccct ttcggtggtg tcggaatcgg gctatttcag aagaggcaaa aacccctccc
    14221 tgccaggcaa gaccgaaagg cagcatcctg aatatgccgc cctgcgtgtc ggaacatggt
    14281 caagccttcg gttggaattc aaaacaaagt gccgcattcg ggctttcccg atgcggcttg
    14341 tcggcacaaa tcaatcgact tttgcggcaa tcgcattgcg ttccacttcg acctcgattt
    14401 ttgtaccctg tccgatatcc acggtaaaaa actgttcgcc gactctggtt accttaccct
    14461 tgaaacctgc cgccaagacc actttgtcgc cggctttcaa ggcggcaagc attgcctgat
    14521 gcgctttgaa tttcttttgt tgggggcgca tgatcaggaa gtagaacacc accataatca
    14581 acaccaaagg agcaaattgt gcaacagctt gattcataat ttatccgttc tttctaaata
    14641 tgattgaaaa tcgagcgggg tatataatag cataagaccg taaacaatat atcgggtttg
    14701 ccgcccatac ccaaccgtat gccgtctgca ccccctttcc cgaacccatg ccgctgaccc
    14761 gcctgttcct gaaactctac gccctgttgc gccttttttt gggcaaaaac gcccagaccg
    14821 cctggatttt ccaccccgcc tgcaccgggc acgaccccgg ggcaaaccat cccgattcgc
    14881 ccgaccgcat cctctgcatc gagcaggcat tgcgccgcgc cggtatttgg cagcacctcc
    14941 aaaccgtaga ggcggaagaa atcagcgata cgcgccttgc acttgtccgc tcgagcaaat
    15001 atctgaaccg tttggaatct tgcctacccg aagacggcaa gatttgccgt ttggacgacg
    15061 acaccgttat cagcaaaaac tccctgtccg ccgcccgttt ttccgccggt tcggcagttc
    15121 aggcagtcga tatggtcatg aaacgcaaag cgtggcacgc cttttgcgcc gcccgcccgc
    15181 cggggcatca cgcaaaaagc ggcaaagccg gcggattctg cctgctgaac aacgttgccg
    15241 ccggcgtcat gcatgccatt gccgaatacc gcctgaaacg cattgccgtc atcgatttcg
    15301 atgtccacta cggcgacggt acggcagaaa tattcaaaga cgatccgcgc atcctgtttt
    15361 tcaacctgtt tgaaaccgac cttttcccct tccccgaaaa caacggtatg cccgacggcg
    15421 gcaatatggt gcacctgccc ttgccgtcgg gaacgggcag ccgcacattc cgcgaagccg
    15481 tccgcagaca gtggctgccc cggcttgccg cattcaaacc cgaactggtg ctgctgtcgg
    15541 caggattcga cgcacaccgt ctggacgaat cgggcaggct caacctgcac gaggcggatt
    15601 ttgcctggct gacacacaaa atcattcaga cggcatccag ctgccccggc aaaatcgtat
    15661 ccgtactcga aggcggctac acccttgaac ctctggcaca atctgctgcc gagcatatcc
    15721 gcgtactggc ggggctgggc aaatccgatg ccgcaaccgc ctatcagaaa acactgaacc
    15781 caacaaaaaa acggtttgcc aaaccgaaaa ccggacaggt gcgccaacct acccaatccg
    15841 accgttacga tataaatgcc gtctgaagcc gattcggttt tcagacggca tcattatttg
    15901 acctgacgca aacagaaagc cttccgcgtg cttaaacaca tctct
//
LOCUS       DAXDSD010000053        15927 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXDSD010000053 DAXDSD010000000
VERSION     DAXDSD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15927
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.053"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..956
                     /locus_tag="ACFB2Q_001256"
     CDS             <1..956
                     /locus_tag="ACFB2Q_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFC0918283.1"
                     /translation="LSDMFYVNYGRSIGGTPDEENFDGHRKEGGSNNYAVHYSAPFGK
                     WTWAFNHNGYRYHQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLSVKLWTRE
                     TKSYIDDAELTVQRRKTTGWLAELSHKGYIGRSTADFKLKYKHGTGMKDALRAPEEAF
                     GEGTSRMKIWTASADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRG
                     FDGEMSLPAERGWYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLSGQTLAGTAIGI
                     RGQIKLGGNLHYDIFTGRALKKPEYFQTKKWVTGFQVGYSF"
     gene            1028..1342
                     /locus_tag="ACFB2Q_001257"
     CDS             1028..1342
                     /locus_tag="ACFB2Q_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918284.1"
                     /translation="MFGAVPDNRVKFPLAHYFKIEILYFISLLLKRCSHFFIGFIIHH
                     LIYGFWRQGLTAFAAVIDFWNRGNAFKESKAIRPDADGGAIIHLLEFVAIGADSCDDY
                     VE"
     gene            complement(1718..2758)
                     /gene="mutY"
                     /locus_tag="ACFB2Q_001258"
     CDS             complement(1718..2758)
                     /gene="mutY"
                     /locus_tag="ACFB2Q_001258"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC0918285.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRTAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2921..3961
                     /locus_tag="ACFB2Q_001259"
     CDS             2921..3961
                     /locus_tag="ACFB2Q_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFC0918286.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKIQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPPHDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKPAKELGATHTISP
                     ASGDVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEILMKAIFSSSVDYTKMLTHRFKFSELEKAYDVFKHAA
                     ENQAMKVVLEAD"
     gene            4059..4171
                     /locus_tag="ACFB2Q_001260"
                     /pseudo
     CDS             4059..4171
                     /locus_tag="ACFB2Q_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4182..4367
                     /locus_tag="ACFB2Q_001261"
     CDS             4182..4367
                     /locus_tag="ACFB2Q_001261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918287.1"
                     /translation="MGYFGGNGGYLKRKQPKNLWLDVGCLKKRNFAKVSERYFLTTVN
                     LLVSAAHHGILPIGRFL"
     gene            complement(4556..5194)
                     /locus_tag="ACFB2Q_001262"
     CDS             complement(4556..5194)
                     /locus_tag="ACFB2Q_001262"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC0918288.1"
                     /translation="MSKLTPRQILTAGAVVPVMAIDDLSTAVDLSRALVEGGIPTLEI
                     TLRTPVGLEAIRLIAKEMPNAIIGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAG
                     RNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(5403..7238)
                     /gene="edd"
                     /locus_tag="ACFB2Q_001263"
     CDS             complement(5403..7238)
                     /gene="edd"
                     /locus_tag="ACFB2Q_001263"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC0918289.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVVKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            complement(7322..7440)
                     /locus_tag="ACFB2Q_001264"
                     /pseudo
     CDS             complement(7322..7440)
                     /locus_tag="ACFB2Q_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7758..9203
                     /gene="zwf"
                     /locus_tag="ACFB2Q_001265"
     CDS             7758..9203
                     /gene="zwf"
                     /locus_tag="ACFB2Q_001265"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC0918290.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
     gene            9354..10049
                     /gene="pgl"
                     /locus_tag="ACFB2Q_001266"
     CDS             9354..10049
                     /gene="pgl"
                     /locus_tag="ACFB2Q_001266"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC0918291.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            10030..11016
                     /locus_tag="ACFB2Q_001267"
     CDS             10030..11016
                     /locus_tag="ACFB2Q_001267"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC0918292.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            11065..11913
                     /gene="hexR"
                     /locus_tag="ACFB2Q_001268"
     CDS             11065..11913
                     /gene="hexR"
                     /locus_tag="ACFB2Q_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC0918293.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            12139..13785
                     /gene="pgi"
                     /locus_tag="ACFB2Q_001269"
     CDS             12139..13785
                     /gene="pgi"
                     /locus_tag="ACFB2Q_001269"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC0918294.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDRHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            14563..15015
                     /locus_tag="ACFB2Q_001270"
     CDS             14563..15015
                     /locus_tag="ACFB2Q_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFC0918295.1"
                     /translation="MALIKEPLDKAKQRNEKLEAAEATAQEAREAEEAAAQEALGREQ
                     EAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGE
                     TPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELPKRL"
     gene            15045..15200
                     /locus_tag="ACFB2Q_001271"
     CDS             15045..15200
                     /locus_tag="ACFB2Q_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918296.1"
                     /translation="MAETVMPQYRNWQSGLDAKCADGDEYGVAQSDCRTREINAKTKE
                     IQGYLID"
     gene            15305..15688
                     /locus_tag="ACFB2Q_001272"
     CDS             15305..15688
                     /locus_tag="ACFB2Q_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC0918297.1"
                     /translation="MIYYPAVLFQDPDQSNWGVIIPDLPGCYPVGDTVADALADAKAA
                     AMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVEADDAALTGQVRFNVSWPQ
                     HILNRVDACTAARHETRAVFWRKPP"
     gene            complement(15772..15906)
                     /locus_tag="ACFB2Q_001273"
                     /pseudo
     CDS             complement(15772..15906)
                     /locus_tag="ACFB2Q_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
BASE COUNT         3882 a         4016 c         4327 g         3702 t
ORIGIN      
        1 gactgagtga tatgttctat gtaaattatg gacgttcaat tggcggtacg cccgatgagg
       61 aaaattttga cggccatcgc aaagaaggcg gatcaaacaa ttacgccgta cattattcag
      121 cccctttcgg taaatggaca tgggcattca atcacaatgg ctaccgttac catcaggcgg
      181 tttccggatt atcggaagtc tatgactata atggaaaaag ttacaacact gatttcggct
      241 tcaaccgcct gttgtatcgt gatgccaaac gcaaaaccta tctcagtgta aaactgtgga
      301 cgagggaaac aaaaagttac attgatgatg ccgaactgac tgtacaacgg cgtaaaacca
      361 caggttggtt ggcagaactt tcccacaaag gatatatcgg tcgcagtacg gcagatttta
      421 agttgaaata taaacacggc accggcatga aagatgctct gcgcgcgcct gaagaagcct
      481 ttggcgaagg cacgtcacgt atgaaaattt ggacggcatc ggctgatgta aatactcctt
      541 ttcaaatcgg taaacagcta tttgcctatg acacatccgt tcatgcacaa tggaacaaaa
      601 ccccgctaac atcgcaagac aaactggcta tcggcggaca ccacaccgta cgtggcttcg
      661 acggtgaaat gagtttgcct gccgagcggg gatggtattg gcgcaacgat ttgagctggc
      721 aatttaaacc aggccatcag ctttatcttg gggctgatgt aggacatgtt tcaggacaat
      781 ccgccaaatg gttatcgggc caaactctag ccggcacagc aattgggata cgcgggcaga
      841 taaagcttgg cggcaacctg cattacgata tatttaccgg ccgtgcattg aaaaagcccg
      901 aatattttca gacgaagaaa tgggtaacgg ggtttcaggt gggttattcg ttttgatgcg
      961 gttgaagtga ataatgtatg acaagataca caaaagctgc cggttttttg caggcagctt
     1021 ttgttttatg tttggggccg taccggacaa ccgggtcaaa tttccattag ctcattattt
     1081 taaaattgaa attttatatt ttatttcact gttgttaaaa cgctgttcac atttcttcat
     1141 cggttttatc attcatcacc ttatttacgg cttctggaga caggggctta ccgcatttgc
     1201 tgcagtaatc gatttttgga acaggggaaa tgcctttaaa gaaagtaaag ctatccgacc
     1261 cgatgccgac ggaggtgcca taattcatct tttggaattc gtggcaatag gggcagattc
     1321 ctgcgatgat tatgtagaat aaaattatga tacctccgaa actgaaatat atcacaaata
     1381 ttatttacat aattgttatt tgcagaaaat tagaacccgt tatcacgtct tggataaatg
     1441 ccaaaaccac tgtgattccg agaattaaaa agaaaaattt cggaatcctc atccttttct
     1501 gaaacctgat tgcggtatcc atggctttgt ctttgtcatt taagtttgtg ttcatgtttt
     1561 tgaccctctt tgtttctaaa atccttttta aattgccgac tttttccgtt ccaaatgtcc
     1621 catttgaaca ttatcccgct gcctaaaata cgacccaaat tcatcggttg ttcttgctgc
     1681 gatacctgct aacaacagtt caacgggttt attttgttta ttctaaccta ttaccgttta
     1741 aataaatttc caaaggcttg ggcaggccgt aatctttcaa atggcccggc tttatccaaa
     1801 tgccgtctga atggtgttcg gacggcattt gcccttcaaa cggcgtaatc atcagcagcc
     1861 ggtgcgtcag gcggtgggtc agggcggttt gttcgtccat atccgccatc gtcagggaga
     1921 gtttggcggc aaagtcggaa agcccgttca aactttcaaa acacggcacg caatacagtc
     1981 cgccccaaat gcctttggcg gtgcgttttt ccagcaagat tgcgccgtcc cggttgcgga
     2041 caatcagcca gtaaagcggc agggtttgca cttcgagggc agttttttta cgcggcagct
     2101 cggcggtgcg gttttgcttt tttgcttcgc agatgtccgc catagggcat tgacggcaca
     2161 agggtttcgt ccgtttgcac acggtcgcgc ccaaatccat caaaccttgc gtgtaagtag
     2221 gcatatcggc gttttcagac ggcatcaggc tttcggcaag tgtccagagc gagttttcaa
     2281 attttttatc ttgcggattg ccgtcttggg caaaaacacg gcagagaacg cgtttgacgt
     2341 tgccgtccaa gatggtttct cgtcggttga aagcaaaggc agaaatggcg gcggcggtgc
     2401 ttctgcctac gccgcagagc gtttccaagt ctttgcgctc cgatggaaac gtaccgccga
     2461 attgtccgac gatttgttgc gcggctttgt gcagattgcg cgcgcggccg taatagccca
     2521 agcccgccca caacgacaac acttcgtctt gcggcgcggc ggcaagcgtc tgaacggtcg
     2581 ggaatttttc caagaaacgc ggatagtagt ccaacacggc ggcgacctgc gtttgctgga
     2641 gcatgatttc ggaaagccag acgcaataag ggtttttgac ctgccaaggg aggtggtggc
     2701 gaccgtgttg tttttgccag cggatgagcc gttcggagaa ggggatgggt gtgttcatta
     2761 atatcaatcg gtggttttat ttatatttaa aacagtatgt tattgtataa aattgtgaaa
     2821 ataattctta ttgacttatt ttttgtaggg gcatataact catataaaga aactttgttg
     2881 cggtattgaa attatttatc aacaagcaag gagtatcagc atgaaagcaa tggtttatca
     2941 cggcgcaaac gacatccgtt ttgaagaaaa accccgcccg cagattatcg atccgaccga
     3001 tgcggtggtg aaaatcgtca aaaccacgat ttgcggtacc gacttgggta tttggaaagg
     3061 caaaaacccc gaagtcgccg acggtcgtat cctcggtcat gaaggcatcg gtattgtaga
     3121 agaagtcggc gaggctgtaa aaaacatcaa agtcggcgat aaagtcatta tttcatgcgt
     3181 cagcaaatgc tgcacttgcg acaactgcaa aatccaactt tattcgcact gccgcaacgg
     3241 cggctggatt ttgggctata tgattgacgg cacgcaggcc gaatacgtcc gcacgcctta
     3301 tgccgacaac agcctcgtgc cgccgcacga caacgtcaac gaagaaatcg ccctgctgtt
     3361 gagcgacgct ttaccgaccg cccacgaaat cggcgtgcaa tacggcgatg tgaaacccgg
     3421 cgataccgta ttcatcgcag gtgccggccc tgtcggtatg tccgccttgt tgaccgccca
     3481 actgtatagc cctgccgcca ttatcgtgtg cgatatggac gaaaaccgtt tgaaaccggc
     3541 gaaagagttg ggcgcgaccc ataccatcag ccccgcttcc ggcgacgtct ccaaacaagt
     3601 tttcgccatc gtcggcgaag acggcgtaga ttgtgccatc gaagcggtcg gtatccctgc
     3661 aacttggaat atgtgccaag acatcgtgaa acccggcggt catatcgccg tcgtcggcgt
     3721 acacggtcaa tccgttgatt tcaaactgga aaaactgtgg attaaaaatc ttgccatcac
     3781 taccggtttg gtcaatgcca acaccaccga aatactgatg aaggcaattt tcagcagctc
     3841 agtggattac accaaaatgt tgacccaccg tttcaaattc agcgaattgg aaaaagccta
     3901 cgacgtgttc aaacacgccg ccgaaaacca agcgatgaaa gtggttttgg aagcggatta
     3961 atcgccgttg aatcagttgt tttttaagcg gatgccgtct gaacgtgttt cagacggcat
     4021 tttttcagcc gtgtaaaacg ggctgttcct gatcaatcag gtatagtgga ttaaatttaa
     4081 atcaggacaa ggcaacgaag ccgcagacag taaaatagta cggcaaggcg aggtaacgcc
     4141 gtactggttt aaatttaatc cactatattt aagggggatt aatgggatat tttggtggga
     4201 acggcggtta tttgaaacga aaacagccga aaaacctgtg gttggatgtc ggctgtttga
     4261 agaaaaggaa ttttgcaaag gtctctgagc gttatttttt aacgacggtt aacttgttag
     4321 tttcagccgc tcatcacggc attttaccga tagggcgatt tttatgacta attgataaat
     4381 atattataat tattttttcg tcataggtct ccgcatatcg cgcgtcattc gcattgttct
     4441 ggagatggat gggcatttta ggggcggtgc tgctgtgtat gccgactgag tcgccaagcg
     4501 gcaaaatgcc gtctgagact ggaaagggct tcagacggca tttttacgat gcggttcagg
     4561 ctttgggttt caatgccgcc gcttctttgg cgaggcgggt aatggtgtcc cagtctttgt
     4621 tttttacagc ttctttcggt gtcagccaag agccgccgac gcataagacg ttgggcaatg
     4681 ccaagtaatc gggcgcagtg gcgaggctga tgccgccggt cgggcagaag cgtacgtcgg
     4741 cgtaaggtcc gtagagggct ttgagcatcg ctttgccgcc aacgacttcg gcagggaaga
     4801 gtttgagtgt gtcgatgccg tgttccaaag ccagttggac ttcgcccgga gtggcaacgc
     4861 cgggaatcag gggaatgccg ctgttgcggc cggctctggc gagggattcg tgcaaacccg
     4921 ggctgatggc gaaaaccgcg cctgcgtctt cgacggcttt gagctgttcg ggattggtta
     4981 ccgtacctgc gccgatgatg gcgttgggca tttctttggc aatcaggcgg atggcctcga
     5041 gtccgacggg ggtgcgcaag gtaatttcga gggtggggat gccgccttcg acaagggcgc
     5101 gggacaaatc gacggcggtg cttaagtcgt caatcgccat cacaggcaca actgcgccgg
     5161 cggtcaggat ttggcggggg gtcagtttgg acatttcggt tctccaagtg gggggtgtcg
     5221 tttttgttga ggtcaaacgg gatggggggg ttaagttttc agacaacctt tggggtaaaa
     5281 cattctgatg ttgcttgggt tttgttcgcg ggcaaagctc acgctacgtc tgcatctatt
     5341 ttaaacggct tcaataattt ttcagacggc cttctgtttt aaaaggccgt ctgaaacgcg
     5401 catcaggcaa attctccgcc gaagctcatc gcaccggttt ccgcactgct ggtcatactg
     5461 cggaagccgg cgaagagttc gcggccgcag ccttgttggt tcgcgcccaa atcgatgcgc
     5521 tcgacttctc ggacgttcca ttcggcctcg ttaatcagga cgttgagttc gccggtaacg
     5581 gagtcgaagc gaatcaggtc gccggtacgg attttggcga tgttgccgcc catcagggct
     5641 tcgggcgtca tgtggatgga cgcgggcact ttgcctgacg cgccggacat acggccgtcg
     5701 gtcagcagcg ccactttgaa gccgcggtct tgcaggatgc ccaaaggcgg ggtcagtttg
     5761 tgcaattcgg gcataccgtt ggcacgcggg ccttggtagc ggacgacgca gataaaatcg
     5821 cgttccaact cgccgcgttc aaatgccgcc aacacttcgc gctggtcgtt gaacacgatg
     5881 gcgggtgctt cgatgatgcg gcagccttcg cgtacggctg acactttaac cacgccgcgt
     5941 ccgatgttgc ctttcatcag gcgcagaccg ccgtccgggg agaacggatt gtcggcttta
     6001 cgcaggatgt cgtcgttgcc gctggtctcg ggggcttcgc gccattcgag tttgccgtcg
     6061 atgaggaaag gctctttagt gtagtggcgc ataccgtgtc cgacgacggt atcgacatcg
     6121 tcgtgcaata agcctgcgtc cagcaattcg cggataacga aaggcaggcc gcctgctgcg
     6181 gtaaagtggt tcacgtcggc tttgccgttg ggatacacgc ggatgagcag cgggatgatg
     6241 gaagaaattt cgtcgaagtc gtcccagttc aaaatcacgc ctgcggcgcg cgccatagcg
     6301 acgaggtgca tggtgtggtt ggtcgagccg ccggtcgcca tcaaaccgat cagggcattg
     6361 atgaaggatt tttcggtcaa catttcgccc aaaggtttaa tcgtgccgtt tttgatgccg
     6421 cgcgcgaggt gtccggcggc gtagcgggtc agggcttcgc gcaggtcggt ataggggtgg
     6481 acaaaggcgg cggcaggcag gtgtacgccc atcatttcca tcatcatttg gttggagttt
     6541 gccgtgccgt agaaagtgca ggtgcccggg ctgtggtaag aacccatttc gcttttcaaa
     6601 agttcgtcgc gtccgacttt gccttcggcg aaaagttggc gggtacgggc tttttcttta
     6661 ttgccgatgc cgctggacat cgggcctgcc ggaacgaaga taccgggaat atgaccgaac
     6721 gaaagcgcac ctatcatcag acccggaacg attttgtcgc acacgcccat aaacaggctg
     6781 ccgtcaaaca tttggtgcga cagcccgacg gcggtactca tcgcaatcac gtcgcgggaa
     6841 aacagcgaca attccatgcc ggcgtagcct tgcgtgatgc cgtcgcacat ggcaggcgtg
     6901 ccgccggcga cttgtgcggt cgcgccgttt ttttgtgctt cgtctttgat ttggtcaggg
     6961 aagtctttaa acggctggtg tgcggaaacc atgtcgttgt aggcagtgat gatgcctaag
     7021 ttggggacgg tttcctgaag catttcgatt ttgatgcttt tgggcatggc ggcataaccg
     7081 tgcgccaaat tgctgcagcc gagctggttg cgctctaagc gtcccatttg tttggcactg
     7141 cggattttcg ccagatattt ttcacgcgtc ggacggctgc gttcgataat gcgcccggtg
     7201 atttcggcga gtttggggtg gataggagtg tggttcatgt tcggtctcct ggcggaacgg
     7261 ggtggtattg gggatttgtt tgtgcgtttc tgatttttgt atcgggtatg ttagacggtg
     7321 agtatagtgg attaaattta aaccggtccg gcgttgcctc gccttgccgt actatttgtg
     7381 ctgtctgcgg ctcgccgcct tgtcctgatt taaatttaat tcactatatt ttaaaaggta
     7441 cgccgtctga aagtgggaag cgggtgctat tatagtacaa aatttgtaat tttgttacaa
     7501 aaaaatcaga cattgaaaag aaatttgatt taaatcgcag aatcttctga ttgttgtaac
     7561 ggaatagggg aaacctgcct gatttttgga ctaggtcaaa taaattgtaa aaatgggaat
     7621 agacaaaaag tgtagcggtg tgtagtatta ctactcatta tgccgcctgc ctttgtttcc
     7681 gatattgcga tgctgcgtga tggaaaaaac aggtttcaga cggcatcgaa aaaatccttt
     7741 cctgacgaga taatgcgatg agtacacaga caaattttga tttggtgttg ttcggtgcga
     7801 ccggcgattt ggcaatgcgc aaacttttgc cctgcctgta tcaggcgcat gttgccggtc
     7861 tgcttcatcc cgaagggcgt attttggggg tcagccgcag cgagttggat accgaaggtt
     7921 ttctggcgaa ggtggaaacc agttccaaaa tccatgtgaa agaaaatttt tccggtgagg
     7981 cgtgggcttc gtttgttgaa cgtctcgcat atctcaaagt cgatgttacg cagccggacg
     8041 attttgccgc tttgggcgat ttggtcaaag cacgcaaaga aaccgacaac gtcgtcatct
     8101 atctgtccac cgcgccgaaa ttcttcgcgc aagcctgcga aaaccttgct gcgatcggtt
     8161 tgaatgccga caatgtgcgc gtggttttgg aaaaaccgct gggtacggat ttggcttctt
     8221 cccaacaaat caataccgat gtggcgcgtt atttcaaaga agggcaaatt taccgcatcg
     8281 accactacct tggcaaagaa agcctgcaaa acctgcttgc gctgcgtttt gccaatgtaa
     8341 tgttcgagcc gctgtggaac aataaataca tcgaaagcgt gcagctgacc atcgccgaac
     8401 agctcggtgt ggaagagcgc ggcgagtttt acgacattac cggcgcgttg cgcgatatgg
     8461 tgcaaaacca tttgatgcag atgctgtgca tgactgcgat ggaagccccc gccagcttgg
     8521 atgccgacgc ggtgcgcgat gaaaaagtca aagtcatcaa gtcattgaag ccgctgacca
     8581 tcgaatctgt caatgaaaat gtcgtgcgcg gacaatatac cgctgccaaa ggcatgaacg
     8641 gctatcttga agaaatcaac gttccgcaag acagctttac cgaaacctac gtcgccatta
     8701 aagccgaaat cgaaaacgaa cgctggaagg gcgttccctt ctacctgcgt accggcaaac
     8761 gcatggcggg caaagtggcg gaaatcgttt tgaacttccg cccgctgcaa aaccatattt
     8821 tcgacaacag ccaaaccgcg ccgaaccgtt tggttatcga gcttcaaccg aatgaatccg
     8881 tccgccttta cacgcaggtg aaaaccccgg gtgcgggcaa caaagtggaa gtaacgcctt
     8941 tgggcgtgga tttgggcaag gccgttgaag gccgccgcgc cgaggcttac gaacgcctgc
     9001 tgttggatgt gattaacggc aaactcgctt tgttcaaccg ccgcgacgaa ctcgaagccg
     9061 cgtgggaata tgtgatgccg attttggaaa actgggcaaa taacaccacg ccgccgcacg
     9121 gttacggcgc acactcttgg ggccctgaag ccgcccgcga actgttggcg cgcgacggca
     9181 acaagtggca cgaagaacag taatgcgctt cagacggcat cgggttcgca atgccgtctg
     9241 aaggagggcg ggcagaaaaa gcagtcccgc ttcgaggcgg aagcggctaa gccattgccc
     9301 ggcagccgcc ctgtaacggg atatgccgtc tgaaatcaca aaaggacaca aatatgtttg
     9361 tttggcacga atacgaaaat gcggcagaag cggcgcagtc tttggctgac gcagtggcgg
     9421 atgctttgca gggcgcactg gacgagaagg gcggtgcggt gttggcagtt tccggcggac
     9481 gttcgccgat tgcatttttc aacgccctgt cgcaaaaaga tttggattgg aaaaacgtcg
     9541 gaattacctt ggcagatgaa cgcatcgtgc cgaccaacca cgccgacagc aataccggtc
     9601 tggtgcgcga atacctgtta aagaacaaag ccgctgcggc agtgtggatt cctatggtgg
     9661 aagacggaaa aactgaaacc gaattacatc ccgatgctgt tgtcgattat gcgctgaaac
     9721 attacaagca gcccgatgtt ttgattttgg gtatgggaaa cgacgggcat acggcttcga
     9781 ttttcccaaa agctccgcag tttcagacgg caatcgacgg ttcggcaggt gtcgcgctgg
     9841 tgcataccac gcccgttacc gcgccgcacg agcgcatcag catgactttg gatgcgattg
     9901 cccatactgg gcatgtattt ttggcgatac agggcgaaga gaaaaaagcc gtgttcgacc
     9961 aagccgcaca aggcgaaaac cgcgaatatc cgatcagcct cgttttgaac catcaaggag
    10021 tgaactgcca tgtcttctac gccgaataaa cacgccgatt acccgaggct ggttgccgac
    10081 atcggcggca caaacgcccg tttcgcattg gaaaccgcgc cgtgcgtgat tgagaaagtc
    10141 gccgtcctgc cgtgtaagga atacgacacg gttaccgacg cggtgcgtgc ctatctgaac
    10201 caaagtggtg caacgggcgt acggcacgcg gcatttgcca tcgccaaccc gattttgggc
    10261 gactgggtgc agatgaccaa ccaccattgg gcgttttcca tcgaaaccac ccgtcaggct
    10321 ttggggctgg acaccctcat ccttttgaac gactttaccg cgcaggcatt ggcggtaacg
    10381 cagacttcaa gcaaagacct gatgcaggta ggcgggcaaa agcctgtcga attcgccccc
    10441 aaggccgtta tcggccccgg taccggcttg ggcgtgagcg gattggtgca cagccccgca
    10501 ggctgggtgg cgttggcggg cgagggcggg cataccagtt tcccgccgtt tgacgatatg
    10561 gaagtgttga tttggcagta cgccaaaaac aaataccgcc acgtttccgc cgaacgtttc
    10621 ctcagcggcg caggtttgag cctgatttat gaaacgctgg cggccaagca gaaagccgaa
    10681 ccggcaaaac tgatgccgtc tgaaatcacc gaaaaggcat taaattgcga atcgcccctg
    10741 tgccggcagg cattggacat tttttgcgcg atgctgggca cggtcgcttc caacctcgcc
    10801 ctgactttgg gcgcgcgcgg gggcgtgtat ttgtgcggcg gcattatccc gcgaatgttg
    10861 gattacttca aaacctcccc cttccgcagc cgctttgaaa acaaagggcg ttttgaagcc
    10921 taccttgccg cgattcccgt ttatgtcgtc ttgagcgaat ttccgggtat tgccggcgcg
    10981 gcggcggcgc tcggcaacca cctgaaaaac gtctaaccac ggcagccctt gcagcagggc
    11041 cgcattatcg aagggcatat cattatgtta agcaaaatca gcgaatcact ggccgacctg
    11101 tccggtgcgg aacgcaaagt tgccgaatgc gccttggcag aacccaagtg gtttgtccac
    11161 gccgccgtcg ccgaaatcgc cgaccgcgcc tcggtcagcc agccgaccgt catccggttc
    11221 tgccgcagcc tcggttataa ggggctgccg gaattcaagc tctccctgtc cgccagcatc
    11281 gggcacgaag gtatgcctta tgtccacgaa gaactcaatg ccgacgacga tatggcgaat
    11341 gtggttgaaa aagtgttggg caacgccgcc gcagccttgt tgggggagag gaggttctta
    11401 aaagaatccg aactcgaaaa cggcatcgcc accctgatgc acgcccgccg cgtcgaattt
    11461 tacggcgtgg gcaattccgg cattgtggca caggacgcgc agcacaaatt tttccgtttc
    11521 ggcatgtcca ccgtcgccta tgtcgatacg cacacgcaac tgatggcggc atccgttttg
    11581 agcgatcagg atgttttggt tgccatttcc aacacgggtt cgtctatcga acttttggat
    11641 gcggtcagca tcgccaaaga aaacggcgcg tccgtcatcg cactgacccg caacgattcg
    11701 cctctggcgc aacttgccga ctgcgtgttg agcgttgcca cacaggaaaa cgccgaactc
    11761 tacacgccca tggtttcccg cctcttgcag cttgccgtca ttgatattct cgccatcgga
    11821 cttgccctgc gcttgggcga tgctgccagc ctgcaattgc agaaaagcaa aaaaagcata
    11881 cacaacaagc acatcgatta cgacaaagac tgatgttcag acggcatccc gccaatgccg
    11941 tctgaacggc cggacaggct ccggcaagcc ttttatcaga atttccttaa tggcggaaac
    12001 cccgcccttt aaggcggcag ggttcgggtt tgccgcaacc ccttccaaat cggggcggta
    12061 cgcagggttg cgccttatgt gcgcatcgat gtgcggaaac gccccccgtc acggaaaacc
    12121 tcaatcggga gcatacagat gaaacacctt cacgacttac cggcctggtc aaaattgtgg
    12181 aaccactttg acgacagcaa aacattgcat atgcgcgaaa tgttcgagca agacccgcag
    12241 cgggcggaac gctactggct gcaagtcggc ggactgacgc tggactactc caaaaaccgc
    12301 atcaacgacg aaaccatgtc cctactgttc gagttggcgc gtgaagcagg cgtgccggag
    12361 cggatgcggc agatgttcca cggcgaaaaa atcaatacca ccgaaaaccg cgccgtcctg
    12421 catgtcgccc ttcgcaaccg caccaattcg ccgattatgg ttgacggtga agatgtgatg
    12481 cccaaagtca accgcgtttt gcaacgtatg ggcgaatttg cacacgaagt ccgcagcgga
    12541 agctggctgg gctataccaa ccaagtcatt accgacgttg tcaacatcgg catcggcgga
    12601 tcggatttgg gcccgctgac gatgtgtacc gcgctcaaac ctttcggtca tccgcgcctc
    12661 aatatgcact tcgtctccaa cgtggacggc tcgcaactgc gcgacgtatt gtccaaagtc
    12721 cacccggaaa ccacgctgtt catcatcgcc tccaaaacat ttaccacgca ggaaacgctg
    12781 accaacgcgc taaccgcgcg cgaatggttt ttgaatcatg cgggtgacga agaagccgtt
    12841 gccaaacact tcgccgccgt ttccaccaat cgaaaagccg tcgccgaatt cggcatcgac
    12901 atcgccaata tgtttgaatt ttgggattgg gtcggcggtc ggtacagcct gtggtccgcc
    12961 atcggattgc cgattatgct gtatctcggc gaagaaaact tcatcgaaat gctcaacggc
    13021 gcgcacctga tggaccgaca cttcatcaac accccgctcg agcgcaacct gcccgtcatc
    13081 ctcgccctca tcggcatctg gtatatcaac tactacggtg gcggcagcca cgtcatcgcg
    13141 ccttacgacc aacatttgca ccgcctgccc aaattcatcc agcagctcga tatggaaagc
    13201 aacggcaaac aggttacgtt ggacggcaaa gcagtcggac acgaaacctc gccgattatc
    13261 tggggagaaa cgggcattaa cggtcagcac gccttcttcc aactgctgca tcaaggcacg
    13321 cacatcaccc ccatcgacct gattgcctcg cttgaaaaac gcagcaacct gcccggacac
    13381 cacgaaatcc tgcttgccaa cgtctttgca caagcagaag cctttatgtg cggcaaaacc
    13441 cccgacgaag tccgcgccga actcaaagcg cagggtatgg atgaggcgcg catcgaagag
    13501 ctggttccgc acaaaacctt ctccggcaac cgcccgacca acctcatcct gatggataag
    13561 gtcaaccccc gcaatatggg cagcctgatt gccatgtacg aacacaaaac cttcgtccaa
    13621 ggcatcattt ggggcatcaa cagcttcgac cagtggggcg tggaactcgg caaacaactg
    13681 gctaaaacca ttttgggcga actgacaggc gaaaccgaac ctcaaaaaca cgacagctcg
    13741 accgaacgcc tgattaacct ctacctgcag accaaccgca aataaaacct gcggaaaaat
    13801 gccgtctgaa cgccgaccgt ttcagacggc atttttatcg aacaggaaaa ccgtcggtaa
    13861 atttgcaggg cgtgtgcaat cgcgatatgg cggtttgcat aaatttaaac atatatgttc
    13921 cgcagccatg tacttcaagt gccgtagcgt gtgtggaaat acgcaacaga cagtccgtgt
    13981 ttggacggca aaggaaatct aatgaaaaaa aatttacccg cactggcatt ggcaagtatg
    14041 ctgattttgt cgggctgcga ccgtttggga ataggcaacc cgttttccgg aaaggaaatt
    14101 tcctgcggaa gcgaagagac taaagagatt ttggtcaaac tggtccgcga caatgtcgaa
    14161 ggtgaaaccg tcaaaacttt tgacgacgac gcattcaaag accaagcatt tgccgatatc
    14221 ggcatatcgc atatccgcag aatggtcgaa cgtttgggca taaccgtcga tgaagtccga
    14281 actaccgaga aaaccgacac gtccagcaaa ctcaaatgtg aagccgcgtt aaaactggac
    14341 gtgcccgacg atgttgtcga ttatgccgtc gccgccaacc aatctatagg caacagctat
    14401 aagaaaacgc ccgacttttt tgaaccctac taccgcaaag aaggcgcgta ttatgtcaaa
    14461 actatttctt acagcgtcca gccgacagac gacaaaagca aaatctttgc cgaactcagt
    14521 caggcacacg atatcatcca tccgctcagc gagctggtgt ctatggcact gattaaagag
    14581 ccgttggaca aagcgaaaca aaggaacgaa aaacttgaag cggcagaagc caccgcgcag
    14641 gaagcgaggg aggcagaaga agcggcggcg caggaggcat tgggtcggga gcaggaagcc
    14701 gcccgcgtat ccgaatggga agaacgctac aagctgtcgc gcagcgagtt cgagcagttt
    14761 tggaaaggat tgcctcaaac tgtacagaat aagctgcaag cctcgcagaa aacatggaaa
    14821 agcggtatgg acaagatctg tgccaacaat gcgaaagccg aaggtgaaac gccaaacggc
    14881 ataaaagtca gtgagttggc gtgtaaaacg gcagaaaccg aagcacgctt ggaagagctg
    14941 cacaaccgta aaaaagccct tatcgacgaa atggtcaggg aagaggacaa gaaagaactg
    15001 ccaaagcggc tctgaacagc gcggttcagg cactgcccgc cgatattgcc gaaaccgtta
    15061 tgccccagta ccgcaactgg caaagcggtt tggatgccaa atgcgccgac ggcgacgaat
    15121 acggggtggc acaatctgac tgccgtacca gagaaatcaa tgcgaaaacc aaagaaatcc
    15181 aaggttatct gattgactga aacttggatg cgggaatgtc ggcggctttt gcgtttttgt
    15241 cgtttttaaa tccccttcgt tgcgtttgga ctttgccgat accgcatatc cgagaaagga
    15301 agtaatgatt tactatcccg ccgtgctgtt ccaagacccc gatcaaagca actggggcgt
    15361 gattattccc gatctgccgg gctgctatcc tgtcggcgat acggttgccg acgcgcttgc
    15421 cgatgccaaa gccgccgcaa tgttccatct cgaagggctt ttgcaggaaa acctgccgat
    15481 tcccgaggcg caaagcatag aggttaatcg ggacaatccc gattatgccg atgcggtgtt
    15541 gtggacgatg gttgaagcgg acgatgccgc attaaccgga caggtgcggt ttaacgtcag
    15601 ttggccgcaa catatcctca accgtgtcga tgcctgcact gccgcccgtc atgaaacccg
    15661 agcggttttt tggcgaaagc cgccttaaag gcgatgcagc cttaactttc agcccgattt
    15721 tttcgggctt ttttattgcc gatgccgtct gaatgcggcg cggcggcaat tttaccgcgt
    15781 atccctttca ttggcggcgc ggctttggtt gaaccgcgcg tcgtgctgcc tgcctttcag
    15841 ttagctgata tacgcctgcc ggcagtggtg gcggtcgaaa aatacgccgt tgatcagggt
    15901 atatatagtg gattaacaaa aaccggt
//
LOCUS       DAXDSD010000054        15271 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXDSD010000054 DAXDSD010000000
VERSION     DAXDSD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15271
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.054"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(191..316)
                     /locus_tag="ACFB2Q_001274"
     CDS             complement(191..316)
                     /locus_tag="ACFB2Q_001274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918298.1"
                     /translation="MPPETDFAKRRRVTGVEMETERVKPDVSVLRKPAIKGLAGV"
     gene            356..592
                     /locus_tag="ACFB2Q_001275"
     CDS             356..592
                     /locus_tag="ACFB2Q_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFC0918299.1"
                     /translation="MLRLQKWGNSAAVRLPARLLTALNAKIGDALETEIRNGELLIRP
                     VKRYRLSDLLAETEAAPPRAEGWEEMPDVGQEGV"
     gene            592..939
                     /locus_tag="ACFB2Q_001276"
     CDS             592..939
                     /locus_tag="ACFB2Q_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC0918300.1"
                     /translation="MYIPERGDIFHLAFDPAAGTEMKGGHYAIALSPRAYNRATGLVY
                     ACPISQGRAAAARSGGMISTLLGAGTATQGNVHCHRMKALDWKIRRAAFRETVPDYVI
                     EDVLARIGAVLFD"
     gene            complement(994..1146)
                     /locus_tag="ACFB2Q_001277"
     CDS             complement(994..1146)
                     /locus_tag="ACFB2Q_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918301.1"
                     /translation="MKFSINREFRKMLKLFAEYAVLRRMFYAVLCAAPLTALIWKPAD
                     ILNALK"
     gene            complement(1305..1637)
                     /locus_tag="ACFB2Q_001278"
     CDS             complement(1305..1637)
                     /locus_tag="ACFB2Q_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918302.1"
                     /translation="MGFLQFGLLFAAAGGVLGAVWASLQERGRPVQAVLEAFISAIAA
                     AAAAERFVPLDQAWTCAAAGVFAGMMTGHALDTVRALAPKVLRGYLGGLAEKLTGVKD
                     GGSSDGKD"
     gene            complement(1638..2108)
                     /locus_tag="ACFB2Q_001279"
     CDS             complement(1638..2108)
                     /locus_tag="ACFB2Q_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918303.1"
                     /translation="MLRFLTERRFLPAGFQAWLFGTATRVLEAVSGLGLSGYAAVFAL
                     APDEIYAWRIYYKFQDIPEAWTVGVLAAAGLLQTALLFARGVRACVASAYLLLFSGFV
                     WFLVSVAFWGAYPPLNTGMVVPPLLAFFCALAGNNALRFLFSAQKSRGLADEGS"
     gene            complement(2179..2484)
                     /locus_tag="ACFB2Q_001280"
     CDS             complement(2179..2484)
                     /locus_tag="ACFB2Q_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918304.1"
                     /translation="MIEDKKIVALNFAVEDGMTGASAAYHVVEHIGADYRNGYVTATL
                     NGYVSENAFKSGKRYLLARTLDFQETDITAPDPGWVYRKALEGAAGIPKDAQPVYAE"
     gene            complement(2596..6741)
                     /locus_tag="ACFB2Q_001281"
     CDS             complement(2596..6741)
                     /locus_tag="ACFB2Q_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC0918305.1"
                     /translation="MGGKSSTVTSAEERILSLQVQRSSQGLTLPVIYGRTRVAGNLVW
                     YGDFVTIEHKTTTRRGGKGGGGVKQVGISYTYEAAVMLALCEGEIQGVGRVWRDKEKF
                     DSPAQLRLTLMRGGDEQPLWTHLQQAKHQGQALNYSGTAYLCSPNYELTKSAQIYQHN
                     FEVIGKSGYSGNIPDANPREIVLDLLTNQRYGCGFPSQNIGDTDRYSNYCRAVGIFLS
                     PAYTEQGEAQRNISELLEQTNSAAVFSQGRLKIIPYGDGSHSGNGAVYVADNKAAYDL
                     TDDDFIVSGAQDPVKAGRKTNADAFNQIQVEYLDRDNDYNVAIAEAKDQANIEQYGLR
                     PKDAVKMHGICDAKVAQKVAQQLLQRALYVRNEYEFKLGWKYCLLEPMDIVTLTDAGL
                     GLNKTPVRITEIEEDGEGVLSVKAEDCPAGVYTVSEYPTQPSSGYSADYNVSPGNAHV
                     PVIFEAPLQLTGGEPQIWLATAGGGMWGGAEVWVSADGDSYTRVGAVNRKARFGALTA
                     DLPDGAVFDRTNTLGVEISAGQLTGGTEQDSRDLLTLCYVDGEFLAYADAELKGVGRY
                     TLGNLTRGAYGSAVNAHAAGSRFARIDEALFKYAVPRNWIGRTVWVKLVSYNVFGGGI
                     QDLAEVPAYSYTIEGAPLGQIQNLRLTSSWAYGKEAVIAWDKLDGADTYDVEIYAGGS
                     RRRLRAVDGIVDNSYTYTQADMKADGGQVRGIVFKVRGRAVTGKTGNWAQIAAQNPQL
                     QALQGISIDSGLRQAFFTCQKPDEEDFAGIIVWVCENAACPAADANKAYDGAETFITI
                     AKCGGKPLEKGKTYYLRAAGYDSFGKDNLKISGSISFTVYDVSATDLSESSLNKALRD
                     KINLIDGNGAGSVNERVEAVRSTADGNAAAVQTHARSINGLEAQYTVKVDANGKVAGF
                     GLATTPKNGTPESKFIVNADRFGIGAAGKADVFPFVVDTQKNRVGINGELVVNGKAVV
                     DKLNAGDIHGGKIAADTLDANRLKAGSVTAREIGAAAVTADKIGANAVTADKIQVADL
                     SAVSSNLGSITGGSLNIGGGNFTVSSDGILTADNAVIRGRIEADSGYFNGTVRASSVE
                     GDVLRAHRLRWTEGNVWVLDLDKDPLPRVLIPNFYVVSETFGNNRVQAKLLLNGGVLA
                     PREVRETENYTNYIWRGRTFGRYEDLPPRAMGGGDYTKQSGSRYRTRLEYQIQVIPAG
                     KPVSLKLSLASHESVFSPFVSVSYLAQSDYEYKQLLGRMVWRTFAESFRYDSKKQVYL
                     GGDRRVHDYQNQMYKHWNAYGGLLQLPDDIYGISFEYRPFTNADWSTMLAFDRSDKFV
                     VVKKYRGHAPQQYSLFQEEFNTAVPKSNLLFFVEHWWQYIELRNIRVLIPESRENEVW
                     PSV"
     gene            6981..7259
                     /locus_tag="ACFB2Q_001282"
     CDS             6981..7259
                     /locus_tag="ACFB2Q_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC0918306.1"
                     /translation="MESQTFASVFDALCDTPAQAANMRLRAGLMMHIADTVRENGWTQ
                     KQAAEHCGLTRPRINDLLNGKIDKFSLDALVNINAGLGQCISLSFAPA"
     gene            complement(7287..7718)
                     /locus_tag="ACFB2Q_001283"
     CDS             complement(7287..7718)
                     /locus_tag="ACFB2Q_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFC0918307.1"
                     /translation="MPSETDLRARIVEEARSWLGTPYHHHAMVKGAGVDCAMLLVAVY
                     GAVGLLPEGFDPRPYPQDWHLHRDCERYLGFVTQFCRETESPQAGDIAVWRFGRSFSH
                     GGILAGGGKVIHSYIGRGVVSDDIGQAELIGRGVRFFTFSF"
     gene            complement(7720..8574)
                     /locus_tag="ACFB2Q_001284"
     CDS             complement(7720..8574)
                     /locus_tag="ACFB2Q_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFC0918308.1"
                     /translation="MKAATKELIDLLHGGDEFQMADLYTITLSGGRVLRHTGADMPVV
                     WDGQAYGAHELVIKRGATRTAVGLEVDSNTLQISAAPDYRLEGLQWAEAALGGVLDGA
                     RVRIDRVFFDAGLRPVGAVNIFSGRVSDVSGGRSSVKVDVKSDIELLNVSSPRNIYQA
                     GCMRTLYDDGCKVNREKFTVNGRVTENSRTGTVLKHNLTQPDGWFSQGVIKFAGGRNA
                     GLSRTVKAHGGNTFELALRLPYPPQAGDAFKVYPGCDKRRDTCKDKFDNIVHFRGFPF
                     IPSADTVV"
     gene            complement(8571..9170)
                     /locus_tag="ACFB2Q_001285"
     CDS             complement(8571..9170)
                     /locus_tag="ACFB2Q_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HFC0918309.1"
                     /translation="MGNAVFPEFPGLKWGRKKTAVWSTGTQKSASGREFRTAYYTYPQ
                     WRFSLSFEVLRTKASVNELEKLAGFFNARKGSFESFLYEDPADNAVTDQPVGNTVQGV
                     ARYQLVRSMGGFIEPVSAVKERPAVKVGGTALAYGRDYTVTDKGVLVFNTPQPPGRPI
                     TWTGGFYFRVRFTSDTVDFENVLGSLWAAKKIEFTSVKL"
     gene            complement(9170..9436)
                     /locus_tag="ACFB2Q_001286"
     CDS             complement(9170..9436)
                     /locus_tag="ACFB2Q_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918310.1"
                     /translation="MIAHVCASTGWTWDYVADNLDLPRIKHLNEYWREHPPVHILAAS
                     YMGVKPSSGSVQSEADEAEAVGMLGGGELSEDEFDALLKAKGII"
     gene            complement(9448..9777)
                     /locus_tag="ACFB2Q_001287"
     CDS             complement(9448..9777)
                     /locus_tag="ACFB2Q_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918311.1"
                     /translation="MTVRIKGVTVELNGADYVIPPIALGALEQLQERIGSFDGNAADA
                     GQISTVIDCAHAALKRNYPDLTREEAADLIDIGNMNEVFAAVMDVSGLKRREQEAAQA
                     GEARAAD"
     gene            complement(9838..10611)
                     /locus_tag="ACFB2Q_001288"
     CDS             complement(9838..10611)
                     /locus_tag="ACFB2Q_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918312.1"
                     /translation="MQLTFGSGEVFAQMITDAYGNRVQNATPVRIMGLQEMSVDLSAE
                     LKEFYGQNRYPLAVAQGKVKVSGKMKGALINGLTLNTLFFGTEYATGTMKALWAETTG
                     KVLDGDNYSYLHAAAPGGGKFAEDAGVMGQDGTAYIKVASSPKQGQYTVSESGVYAFN
                     SSDKGKTVYPSFTYTQTMPPAKKIELTNMATGNTPTFKMRYLTQFKGKKALLELESVT
                     SGKLGLFSTKNDDFSVPEIDFTASTDEAGFKIGTLWIQE"
     gene            complement(10637..11065)
                     /locus_tag="ACFB2Q_001289"
     CDS             complement(10637..11065)
                     /locus_tag="ACFB2Q_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918313.1"
                     /translation="MNREAVYSALWSKLDALDGFVTKSRKLVHWNDVKRYDQPALFMA
                     QGDMQALTLTGRETKWILRADVYLYVQTAGQPPAPVMNPLIDAVCNAVNAVHPVTGKT
                     DLTADGADIEYCRVEGTVETDEGTLGEQAVCIIPIVICAA"
     gene            complement(11062..11544)
                     /locus_tag="ACFB2Q_001290"
     CDS             complement(11062..11544)
                     /locus_tag="ACFB2Q_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFC0918314.1"
                     /translation="MLKMEFIGGGILAAVFKAYAADVQDAVVKSVGRSALRLQREVKQ
                     NRLSGRVLRVRTGNLRRSVHQRVNVSGNVVSGEVDTNVRYGIAHEYGFAGSVNVKASL
                     RQVRQAFGKPLKPPRYVRVRAHTRDVKLPERSFLRSALRDLTPKFADDLQKSIGKVLK
                     "
     gene            complement(11544..12074)
                     /locus_tag="ACFB2Q_001291"
     CDS             complement(11544..12074)
                     /locus_tag="ACFB2Q_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFC0918315.1"
                     /translation="MAAPVSLEEFKQRIGVEHDRRDDFFLSVIDGVSAAAEAYIGRSL
                     LAADYVGRYDGNGKDRIVLDNYPVLSVSSVKINGADAGGWEFDNWLLMRPEGFARGLK
                     NVEVSYRAGYERMPADIREAVMIIAVQRVNEIEGKGVRSKTLAGETVAFSTFGNSGGM
                     PPSAFAILNEYKRKGV"
     gene            complement(12077..12439)
                     /locus_tag="ACFB2Q_001292"
     CDS             complement(12077..12439)
                     /locus_tag="ACFB2Q_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918316.1"
                     /translation="MPSENQTAFSFGDFEMTVKLKAPEGFTDVSFGSQSYAADENGIV
                     EVPSEAAEFLYQFGFGNVASEPAEGPEKAKRGRKPKTGQPAGQSEPAEAAEPAEAEAE
                     AAEPAEAEAEPAEAEKAE"
     gene            complement(12446..13945)
                     /locus_tag="ACFB2Q_001293"
     CDS             complement(12446..13945)
                     /locus_tag="ACFB2Q_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918317.1"
                     /translation="MNVNQLTQETIELMKSAQAGGGPPDKGFTQPAGFTAGLQTYDLS
                     APSQKLYPVLTPLRNRIPRVGGGRTIGSNWKAVTNINVGNQRAGIGEGRRGGVINHET
                     VERNAQFRAIGLENQVTFEADYAARGFEDVKALAVAQTLQATMVAEEMILLGGNTSLK
                     AGVTPTPTAVVSADAAGKISVSTLSVICVALGLQAYWDVAGANNGATGQSPNIKTAQV
                     PAKITRRNADGTTDTFGGGSARKSAAASVSGIEAGKKVTAVIPAVRGAVAYAWYWGAA
                     GSEKLGAVTTAAKVEISADAEGTQTAASLPSEDNSTSILEFDGLLTQIALPDSGAFWS
                     DNKGGGLTSDNAGGVYEFEEAFAHFFTRYRLSPDTVYVNARDLAALTKLIIGNSGAPL
                     IKLNVDVNNTANIRAGVVVGSYMNKITGDDLNIVVHPNLPAGTYLFYSSRLPAYVQGV
                     GNLLQVRTRQEYYQIEWPLRTRMYEYGVYADEVLQGMFMPAFGMITNVG"
     gene            complement(13986..15143)
                     /locus_tag="ACFB2Q_001294"
     CDS             complement(13986..15143)
                     /locus_tag="ACFB2Q_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC0918318.1"
                     /translation="MTKLYAQIAKTEAQDDGTVKVWGYASSEAVDSDGEVVAAEAMKA
                     AIPDYMKFGAVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVTKVKTGVYKGFS
                     IGGRVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKPKDEAGAADKDGKP
                     SDKPTEEEDENPKDGDKGPKTEDKGDKDAGKKDEAGKSASVELSESEIAALKAVLAKA
                     DKPKGGTAAKSMYQVKSPADVLMSLKWLVEDASYDNIDEAVTAQIKESAAGLAESLKA
                     LAASEADKPADGLAAKAGKSGDLAKAESADELAKAQDALKKSNDALAKAQAEIESLKK
                     QAVPPKGSTKAISKAEDNGEDPLKGFQPIVKNDGTLDDVATLIKAKQTGRL"
BASE COUNT         3199 a         4828 c         3920 g         3324 t
ORIGIN      
        1 tctgtttctt tccaccacgc tttctgctcc tgattaaaat ttcccatttc ttgcagcatc
       61 gctaaaagca cagggtttga cttgccattc actttttcgt gagcgccaag gtatttacgg
      121 gcttcttcaa tccatttcaa ttctttcatt tcttttcctt ccgataaaag acgagatgta
      181 aactttcggt ttacaccccg gccaagcctt tgattgcagg tttccgcaac accgatacat
      241 ccggtttcac acgttccgtt tccatctcca ctcctgttac acgccgccgc ttggcaaaat
      301 ccgtttcagg cggcataatg tatcgacaac gtatctacaa cacgaaagca aaaccatgtt
      361 gcgactgcaa aaatggggca acagcgcggc agtccgtctg cccgcccgcc tcctgaccgc
      421 cctcaacgcc aaaatcggcg acgcgctgga aacagaaatc cgcaacggcg agctgctcat
      481 ccgccccgtc aaacgctacc gcctgtccga ccttttggcg gaaacggaag ccgccccgcc
      541 gcgcgcggaa ggctgggaag aaatgcccga tgtcggacag gagggcgttt gatgtatatt
      601 cccgagcgag gcgacatttt ccatcttgcc ttcgaccccg ccgccggtac ggaaatgaaa
      661 ggcggccatt acgccatagc cctgtcgccc agagcttaca accgcgcaac cggcttggtt
      721 tacgcctgcc cgatttcaca aggcagggcg gcggcggcaa gaagcggcgg tatgatttcc
      781 acactgctcg gcgcgggaac ggcaacacaa ggcaatgtcc actgccaccg gatgaaggca
      841 ttggattgga aaatccgccg cgccgccttc cgcgaaaccg tccccgatta cgtgattgaa
      901 gacgtattgg cacgcatcgg cgcagtactg ttcgactgac ttcagagtgc gaagcctgtc
      961 cgcaggggga agcatccgtt tccatacccg cccctatttc aaagcattca aaatatccgc
     1021 cggcttccaa atcaacgccg tcaaaggcgc ggcgcacaaa accgcataaa acatacggcg
     1081 caacaccgca tactcggcaa acagcttcaa catcttgcga aactccctgt ttatgctaaa
     1141 cttcaaacag atttctcctt acctcggata aggttaaatt cagaaacgcc gcaaggattc
     1201 cgcccctcgc ggcgtttcgc cgttcacacc ccgcgcgtaa tgtcgtgcgt cagccggacg
     1261 cgcccggaac acagcgtctt cacgcgccgc gataccgctc gaaatcaatc ttttccatcc
     1321 gaagaccctc cgtctttcac acccgtaagc ttttccgcca agccgcccag ataaccgcgc
     1381 aacaccttgg gcgcgagcgc gcgcaccgta tcgagcgcgt gccccgtcat catccccgcg
     1441 aacacccccg ccgccgcgca cgtccacgcc tgatccagcg gcacgaaccg ctccgccgcg
     1501 gccgccgccg cgattgcaga aataaaagcc tccagcacag cctgcacggg gcggccgcgt
     1561 tcctgaaggc ttgcccacac cgcgcccagc acgccgccgg ccgcggcaaa cagcagtccg
     1621 aactgcaaaa accccattca cgacccctca tccgccaaac cccgcgactt ttgcgccgaa
     1681 aacaaaaacc tcaacgcatt attccccgcc agcgcgcaga aaaacgccaa cagcggcgga
     1741 acgaccatac ccgtgttcaa aggcgggtac gccccccaaa acgccaccga aaccaaaaac
     1801 cacacaaagc ccgaaaacaa aagcaggtag gccgaagcca cacaagccct cacgccccgc
     1861 gcaaacagca atgccgtctg aagcagcccc gccgccgcca gcacccccac cgtccacgcc
     1921 tccggaatat cctggaattt gtaataaatc cgccacgcgt aaatctcgtc gggcgcaagc
     1981 gcgaacaccg ccgcgtaacc cgacaagccc aaaccgctga ccgcctccag cacccgcgtc
     2041 gccgtgccga acaaccacgc ctgaaacccg gcgggcaaaa accgccgttc cgtcaaaaac
     2101 ctcaacatac ccgtcacaca ccccccggca aaacaccgcg aagccgaagc cccgcagtgt
     2161 ccgacgccgt ctgaaaactt attccgcgta aaccggttgc gcgtctttgg ggatgcccgc
     2221 cgcaccttcc aacgccttgc ggtacaccca accggggtcg ggcgcggtta tgtccgtttc
     2281 ctgaaaatcc aatgtgcgcg ccaacaggta gcgtttgccc gatttaaacg cattttccga
     2341 cacatagccg ttcaacgtcg ccgtaacata accgttgcgg taatccgcgc cgatatgttc
     2401 gacgacgtgg taggccgcac tcgcgccggt cataccgtcc tcgactgcaa aattcaaggc
     2461 aacaatcttt ttatcttcaa tcatttcaaa tctccaaaag cccgctaggg gcgggaacga
     2521 aaatgccgtc cgaggtcttc agagggcatt gcccggacaa aacaggaaaa accgcagagc
     2581 cggcatacgg gggcgtcaga cggagggcca aacctcgttt tcgcgcgatt ccggaatcag
     2641 caccctgata ttgcgcagct cgatatactg ccaccagtgt tccacaaaaa acagcaggtt
     2701 ggatttcgga acggcagtat taaactcctc ctgaaacaga gaatactgtt ggggcgcatg
     2761 accccggtat ttcttaacca ctacaaactt atccgaccgg tcgaacgcaa gcatcgtact
     2821 ccagtcggca ttggtaaaag gccggtactc gaacgatatg ccgtagatat cgtccggcaa
     2881 ctgaagcagc ccgccgtaag cattccaatg tttatacatc tgattttggt aatcgtgtac
     2941 acgccggtcg ccgcccaaat aaacctgctt cttactgtcg taccggaaac tttccgcaaa
     3001 ggtgcgccaa accatcctcc cgagcagctg cttgtattcg tagtcggatt gcgccaaata
     3061 cgaaaccgac acgaacggcg aaaacaccga ttcgtgggaa gcgagcgaca acttcaggct
     3121 gacgggtttg cctgcgggga tgacttggat ctggtactcg aggcgggttc tgtaccttga
     3181 gccggattgt ttggtataat ccccgccccc cattgcccgg gggggcaggt cttcgtacct
     3241 cccgaaagtt cggccgcgcc aaatataatt ggtatagttc tcggtttccc tgacttccct
     3301 cggcgcaagc acacccccgt tcaacagcag ctttgcctgc accctgttgt tgccgaacgt
     3361 ttccgaaacc acataaaaat tcggaatcaa aaccctcggc agcgggtctt tatccaaatc
     3421 caacacccaa acgttaccct ccgtccaacg cagcctgtgc gccctcaaca cgtcgccctc
     3481 gacggacgaa gccctgaccg tgccgttgaa ataacccgaa tcggcctcaa tccgtccgcg
     3541 tattaccgca ttgtcggccg tcaggatgcc gtctgaagac accgtaaaat tacccccgcc
     3601 gatattcagg ctgccgcccg tgatgcttcc gagattggaa gataccgcgc tcaaatcggc
     3661 aacctgaatc ttatcggcag tcaccgcgtt tgccccgatt ttatcggcgg taacggcggc
     3721 agccccgatt tcccgcgccg taacgcttcc ggctttcagg cggtttgcgt ccagcgtgtc
     3781 cgccgcgatt ttgccgccgt ggatatcccc ggcgttcaac ttatcgacaa ccgccttgcc
     3841 gttgaccacc aattcgccgt tgatgcccac gcggtttttc tgcgtgtcca ccacgaacgg
     3901 gaacacatcc gccttcccgg ccgcaccgat gccgaagcgg tcggcattca cgataaactt
     3961 gctttcgggc gtgccgtttt tcggcgtagt cgccaagccg aagcccgcca ctttgccgtt
     4021 tgcatcaacc ttgaccgtgt attgcgcctc caagccgttg atgcttcggg cgtgtgtctg
     4081 aaccgccgcc gcattgccgt ccgccgtcga tcggactgcc tcgacgcgct cgttgacgct
     4141 gcccgcgccg ttgccgtcaa tcaggttgat tttgtcgcgc aaagccttgt tcagactgct
     4201 ttccgacagg tcggttgccg atacgtcgta aacggtaaac gatatgctgc cgctgatttt
     4261 caggttatct ttgccgaaac tgtcgtaacc cgccgccctc aagtaatagg tctttccttt
     4321 ctccagcggt ttgccgccgc atttggcgat ggttataaac gtttccgcgc cgtcataggc
     4381 tttgtttgcg tccgcggccg ggcaggctgc gttttcgcaa acccaaacga taatccctgc
     4441 aaagtcttct tcgtcaggtt tttggcaggt aaaaaacgcc tggcgcaggc cgctgtcgat
     4501 agagatgccc tgcaatgcct gcaattgcgg attttgcgcc gcgatttgcg cccaattgcc
     4561 cgttttaccg gtaacggcac gcccgcgaac cttgaagaca ataccgcgta cctggccgcc
     4621 gtccgccttc atatccgcct gagtgtaggt gtagctgttg tcaacgatgc cgtcaaccgc
     4681 acgcagacgg cgtcggctgc cgcctgcgta gatttccacg tcgtaggtat ccgcgccgtc
     4741 caatttatcc caagcgatga cggcttcttt gccgtatgcc cacgatgatg tcaggcgcag
     4801 gttttggatt tgaccgagcg gcgcgccttc gatggtgtag gaatacgccg gcacttccgc
     4861 caaatcctga atgccgccgc cgaaaacgtt gtacgaaacc agtttgaccc aaaccgtgcg
     4921 gccaatccag ttgcgcggca cggcgtattt gaacaatgct tcgtcgatgc gcgcaaaccg
     4981 gctgcccgcc gcgtgtgcgt tgacggcgga gccgtacgcg ccgcgcgtca ggttgcccaa
     5041 tgtgtagcgt cccacgccct tcagttcggc gtcggcgtat gccagaaatt cgccgtcgac
     5101 gtagcacaat gtcagcaaat cgcggctgtc ctgctccgtg ccgcccgtca gctgccccgc
     5161 cgaaatttcc acgcccaacg tatttgtgcg gtcgaaaacc gcgccgtcgg gcaaatcggc
     5221 ggtcagcgcg ccgaaacgcg ccttgcggtt gaccgcgccg acgcgggtgt agctgtcgcc
     5281 gtccgccgat acccacactt cggcgccgcc ccacataccg cccccggcgg ttgccagcca
     5341 gatttgcggt tcgccgcccg tcagttgcaa cggcgcttcg aaaattaccg gcacatgggc
     5401 gttgcccggc gaaacgttgt agtcggccga ataacccgaa gacggctgcg tcggatattc
     5461 cgacacggta taaacaccgg ccgggcagtc ttcggccttg acggataaaa ccccttctcc
     5521 gtcttcttcg atttccgtaa tgcggacggg cgttttattc aggccgagcc ccgcgtcggt
     5581 cagggttacg atgtccatcg gctcaagcag gcagtatttc caacccagct taaactcata
     5641 ttcgttgcgc acgtacaggg cgcgttgcag cagttgttgc gccacttttt gcgccacctt
     5701 cgcatcgcaa atgccgtgca tcttgaccgc gtctttcggg cgcaatccgt actgctcgat
     5761 attcgcctgg tctttcgctt cggcgatggc gacgttgtag tcgttgtccc tgtcaaggta
     5821 ctcgacctga atctgattaa acgcatcggc attggttttg cgcccggcct ttacagggtc
     5881 ttgcgcgccc gaaacgatga aatcgtcatc ggtcaggtcg taggcggcct tattgtcggc
     5941 aacgtacacc gcgccgttcc ccgaatggct gccgtccccg taggggatga ttttcaggcg
     6001 gccttgcgaa aataccgccg cgctgttggt ctgctccagc agttcggaaa tattccgttg
     6061 cgcctccccc tgttccgtgt aggcagggct taggaaaatc ccgacggcgc ggcaataatt
     6121 gctgtaccgg tcggtatcgc cgatgttttg ggacgggaaa ccgcagccgt agcgttggtt
     6181 cgtcagcaaa tccaatacga tttcgcgcgg gtttgcatcg gggatgttgc cggaatagcc
     6241 cgatttcccg atgacctcga aattgtgctg atatatttgc gccgatttcg tcagttcgta
     6301 gttcgggctg cacaaataag ccgtgcccga ataattcaag gcttggcctt ggtgcttcgc
     6361 ctgttgcagg tgcgtccaca acggctgctc gtcgccgccg cgcataagcg tcaggcgcaa
     6421 ctgtgccggc gaatcgaatt tctccttgtc gcgccaaacc cgccccacgc cctgaatctc
     6481 gccctcgcac aaagcaagca tgacggcggc ttcgtaggta taggaaatac cgacctgttt
     6541 cacaccgccg ccgcccttgc cgccccggcg cgtcgtagtt ttatgctcga tggtgacgaa
     6601 atcgccgtac cacaccaaat tgcccgccac gcgcgtcctg ccgtagatga cgggcagggt
     6661 cagcccctgt gatgaccgct gaacctgtaa cgataaaatc cgctcttcgg cagatgtaac
     6721 ggttgacgat ttaccaccca taaaaccacc tcgaaacttg caaaaattat caaaatcgat
     6781 ataatttact tatatcaaaa cagatataaa atatgaaacc gttaaatttt tcaggcggtt
     6841 cattggattg cctgcggcaa ttccccgaaa acgccaaaca ggcggcaggt tatcaacttc
     6901 aagaaaagcc ggaaaaccgc gggcgccgat tcggaatcgg cggaaaaacg ttacaacaaa
     6961 ttgattcggg aaaagaaata atggaaagcc aaactttcgc ctccgtattt gacgcactgt
     7021 gcgacacgcc tgcccaagcc gccaatatgc ggttgcgcgc cggcctgatg atgcacatcg
     7081 ccgataccgt ccgcgaaaac ggttggacgc aaaaacaggc cgcagaacat tgcggcctga
     7141 cccggccgcg catcaacgac ctgctgaacg ggaaaatcga caaattttca ttggatgcgc
     7201 tcgtaaacat caatgccgga ctcgggcagt gcatttcctt atccttcgcc cccgcgtaaa
     7261 gcccgtcctt tcagacggcc gcaaaatcaa aatgaaaatg taaaaaaccg aaccccgcgc
     7321 ccgataagtt cggcttggcc gatgtcgtcc gacaccacgc cgcgcccgat gtagctgtga
     7381 ataaccttgc cgccgcccgc caatatgccg ccgtgcgaaa acgaccgccc gaaacgccat
     7441 actgcaatgt cgcccgcctg cggcgattcc gtttcacggc aaaattgcgt gacgaacccc
     7501 aaataacgct cgcaatcgcg gtgcagatgc caatcttgag ggtaagggcg cgggtcgaac
     7561 ccttcgggaa gcagtccgac cgccccgtag acggcgacca ggagcatcgc gcaatcaaca
     7621 cccgcgccct tgaccattgc atgatgatgg tacggcgtgc caagccacga ccgcgcctct
     7681 tcgacgatcc gcgccctcaa atccgtttcg gacggcatat cacaccaccg tatctgccga
     7741 agggatgaac gggaagccgc gaaaatgcac gatattgtca aacttatcct tgcaggtatc
     7801 gcgccgcttg tcgcagcccg gataaacctt gaacgcatcg cccgcctgcg gcgggtaggg
     7861 caggcgaagg gcaagttcga acgtgttgcc gccgtgtgcc ttgaccgtcc tgctcaaacc
     7921 cgcgttgcgc ccgcccgcaa acttaatcac gccctgcgaa aaccagccgt caggctgcgt
     7981 cagattgtgc ttcagcacgg ttcccgtccg gctgttttcg gttacgcgcc cgtttaccgt
     8041 gaatttctca cggttgacct tgcagccgtc gtcatagagc gtcctcatgc agcccgcctg
     8101 ataaatgttg cgcgggctgg aaacgttcaa aagctcgatg tcggatttca cgtcaacctt
     8161 cacggacgac ctgccgcccg atacgtccga cacgcgccct gaaaaaatat tcaccgcacc
     8221 gacggggcgc agtccggcat caaaaaacac acggtctatc ctgacccgcg cgccgtccaa
     8281 tacgccgccc agggcggcct ccgcccattg caggccctca agcctgtaat cgggcgcggc
     8341 tgaaatctgc agggtgttgg aatccacttc caatccgacg gcggtacggg ttgcgccgcg
     8401 cttgataacc agctcgtgcg ccccgtaggc ctgaccgtcc caaacgacgg gcatatccgc
     8461 gccggtatgc cgcagcaccc ggccgcccga aagcgtaatg gtgtacaaat ccgccatctg
     8521 aaactcgtcg ccgccgtgca gcaaatcaat cagttctttt gtcgccgcct tcatagtttc
     8581 acactcgtaa actcaatctt tttggccgcc cacaagctgc ccaaaacgtt ttcaaaatcc
     8641 accgtgtcag acgtaaacct cacgcggaaa taaaagccgc ccgtccatgt gatcgggcgg
     8701 cccggcggtt gcggcgtgtt gaaaaccaaa acgcccttgt cggtaacggt gtaatcgcgc
     8761 ccgtacgcca acgccgtgcc gccgaccttg acggcgggcc gttccttgac cgccgacaca
     8821 ggctcgataa atccgcccat cgaacggaca agctgataac gcgcaacgcc ctgcaccgtg
     8881 tttccgacag gctggtcggt tacggcgttg tcggccgggt cttcgtaaag gaaactttca
     8941 aagctgcctt tgcgggcgtt gaagaatccc gccagttttt ccaactcgtt tacggacgcc
     9001 tttgtccgca atacctcgaa cgacagcgaa aaccgccatt gcgggtaggt gtagtaggcg
     9061 gttcggaact cacggccgct tgccgatttc tgcgtccccg tactccatac cgccgttttc
     9121 ttccgccccc acttcaagcc ggggaactcg gggaaaaccg cattgcccat cagatgattc
     9181 ctttcgcttt gagcagggcg tcaaattcgt cttcggacag ttcgccgccg ccaagcatac
     9241 cgacggcttc ggcttcgtcc gcttcgcttt gcacactgcc cgacgacggc ttgacgccca
     9301 tatacgacgc tgccaagata tgcacgggcg gatgttcgcg ccaatactcg ttcaggtgtt
     9361 tgatacgcgg caaatccaag ttgtcggcga cgtagtccca cgtccagccg gtagaggcgc
     9421 aaacgtgggc aatcaccgcg ccgaaactta atccgccgcc cgggcctccc ccgcttgtgc
     9481 ggcttcctgc tccctgcgtt tcaagcccga aacgtccatc acggcggcaa acacttcgtt
     9541 catgttgccg atatcgatca aatcggccgc ttcttcgcgc gttaaatccg ggtaattgcg
     9601 cttcagggcg gcgtgggcgc aatcgataac ggtagagatt tgtccggcat ccgccgcatt
     9661 gccgtcaaat gagccgatgc gctcctgcaa ctgctccaac gcgcccagcg caataggcgg
     9721 aatcacataa tccgcgccgt tcagttctac ggttacgcct ttaatccgta cggtcatttt
     9781 tatttccttg actttggttg aataaaaaaa gccgcctttt cagacggcat ataaacatta
     9841 ctcctgaatc cacagcgtac cgattttaaa gcccgcctcg tcggttgagg cggtaaagtc
     9901 gatttcgggg acggagaagt cgtcgttttt agtcgagaac aagcccaatt tgccgctggt
     9961 tacgctttcc agttccaaca gggctttttt gcctttgaac tgcgtcaggt acctcatctt
    10021 aaacgtcggc gtgttgcccg tcgccatatt ggtcagctcg attttcttcg ccgggggcat
    10081 cgtttgcgta taggtaaagc tcggataaac ggttttgcct ttatccgagc tgttaaaggc
    10141 gtaaacgccc gattccgaaa ccgtatattg gccttgtttc ggggaggatg ctactttgat
    10201 ataagccgtg ccgtcctggc ccatcacgcc cgcgtcctca gcgaacttgc cgccgccggg
    10261 cgcggctgca tgaaggtaag aataattgtc gccatcgagg actttgcccg tagtttccgc
    10321 ccaaagcgcc ttcatcgtgc cggttgcata ttccgtaccg aaaaacaggg tattgagggt
    10381 caggccgtta atcaacgcgc ctttcatttt gcccgacacc ttgaccttgc cttgcgccac
    10441 agccaaagga taacggtttt gaccgtagaa ctctttcaat tccgccgaca aatcgacgga
    10501 catttcctgc aagcccataa tccgcacggg cgttgcgttc tgtacacggt tgccgtaagc
    10561 atccgtaatc atttgggcga acacttcgcc gctgccaaac gtcaactgca tgacatccct
    10621 ttcaaaaaaa gccgcattac gcggcgcaaa tcacaatcgg aataatacaa accgcctgtt
    10681 cgccaagcgt cccctcgtcg gtttccaccg taccctcgac gcggcaatac tcgatatccg
    10741 cgccgtccgc cgttaaatcc gtcttgcccg taacggggtg gacggcgttc acggcattgc
    10801 acaccgcgtc aatcagcgga ttcatgacgg gcgcgggcgg ttggcccgcc gtctggacgt
    10861 acaggtaaac atcggcgcgc aaaatccact tggtctcccg ccccgtcaat gtcaacgcct
    10921 gcatatcgcc ctgcgccata aataacgcgg gctgatcgta gcgtttcaca tcgttccagt
    10981 gcaccagctt ccggctcttg gttacgaagc cgtccaatgc gtccagtttc gaccaaagcg
    11041 cggaataaac cgcctcacga ttcatttcaa cacctttcca atcgattttt gcaggtcgtc
    11101 cgcgaacttc ggcgtcaaat cgcgcaatgc cgaacggagg aacgaccgct ccggcaactt
    11161 cacatcacgg gtatgggcgc ggacgcggac atatcgcggc ggtttcagcg gcttgccgaa
    11221 cgcctgcctg acctggcgca gcgaagcctt aacgttgaca ctgcccgcga aaccatactc
    11281 atgcgcgatg ccgtaacgga cgttcgtatc gacttcgccc gaaacgacat tgccggaaac
    11341 gttcacgcgc tggtgtacgg agcgtcgcag gttccccgtc cttaccctca gcacccggcc
    11401 cgataaccgg ttctgcttga cttcgcgctg caggcgcaat gccgacctgc ccaccgattt
    11461 gacgaccgca tcttgaacat cggcggcata tgctttaaaa accgccgcca gaataccgcc
    11521 gccgataaat tccatcttca gcatcacacg ccctttcgtt tgtactcatt gagtatcgca
    11581 aacgccgacg gcggcatacc gccggaatta ccgaacgtag aaaaagcgac ggtttcgcct
    11641 gcaagcgtct tgctccgtac gcccttgccc tcgatttcgt tcacgcgctg caccgcgata
    11701 atcatcaccg cttcgcgtat gtcggcgggc atgcgctcat aaccggcgcg gtacgatacc
    11761 tcgacatttt tcagcccccg cgcgaaacct tcggggcgca tcagcagcca gttgtcaaac
    11821 tcccagccgc cggcatccgc gccgttgatt ttgacggacg acaccgacag gacgggataa
    11881 ttgtccaaca cgatgcggtc tttgccgttg ccgtcgtacc gcccgacata atcggcggcc
    11941 aggaggctgc gcccgatata ggcttccgcc gccgccgaca cgccgtcaat gacgctgagg
    12001 aagaaatcgt cccgccggtc atgttcaacg ccgatacgct gcttgaactc ctcaagcgat
    12061 accggggcgg ccatcgttat tcagcctttt cggcttcggc aggttcggct tctgcttcgg
    12121 caggttcggc agcttcggct tcggcttcgg caggttcggc agcttcggca ggttcggatt
    12181 gtcctgccgg ctgcccggtt ttaggtttac gcccgcgctt ggctttttca ggcccttcgg
    12241 caggctcgga ggcaacgttg ccaaaaccga actgatacag gaattccgcc gcctccgacg
    12301 gcacttccac gataccgttt tcatctgccg cgtagctttg gctgccgaag gaaacgtcgg
    12361 taaacccttc gggcgctttt aatttgactg tcatctcaaa atctccaaaa gaaaaagccg
    12421 tctgattttc agacggcata tcggcttaac ccacgttggt aatcataccg aaggcaggca
    12481 tgaacatacc ttgcagcacc tcgtccgcat agacgccgta ttcgtacata cgggtgcgca
    12541 gcggccattc gatttggtaa tactcttggc gcgtacgcac ttgcagcaga ttgccgacgc
    12601 cctgaacgta ggcgggcagg cggcttgagt agaacaggta agtaccggca ggcaggttcg
    12661 ggtgtaccac gatgttcagg tcgtcgcccg tgatcttgtt catatacgaa ccgacaacga
    12721 caccggcgcg gatgttcgcc gtattgttca cgtcaacgtt caacttaatc aacggcgcgc
    12781 cgctgtttcc gataatcagc ttggtcagcg ccgccaaatc gcgggcgttg acgtagacgg
    12841 tatcggggga caggcggtat cgggtaaaga aatgcgcgaa cgcctcttca aattcataca
    12901 cgccgcccgc gttgtcggaa gtcaggccgc cgcctttgtt gtccgaccag aacgcgcccg
    12961 aatcaggcag ggcgatttgg gtcaacaggc cgtcaaattc caaaatagaa gtggaattgt
    13021 cttcggacgg cagggaagcg gcggtctggg taccttcggc atcagccgaa atttccactt
    13081 tggcggcagt ggtaaccgcg cccagttttt cagaaccggc cgcaccccag taccaagcat
    13141 aggcaaccgc gccgcgaaca gccggaatca cggcggttac ttttttgcct gcctcaatac
    13201 cggaaacgga agccgccgca gattttcggg cggaaccgcc gccgaacgta tcggttgtac
    13261 catccgcgtt tcggcgtgtg attttggcag ggacttgggc agttttaatg ttcgggcttt
    13321 gtccggttgc accgttgttc gcgcctgcca catcccagta tgcctgcaag cccaaagcca
    13381 cacagattac agacaaggtg ctgacgctga ttttccccgc cgcgtcggcg gaaacgacgg
    13441 cggtcggtgt aggtgtaacg cctgccttca ggctggtgtt gccgcccagc aaaatcattt
    13501 cttcggcaac catagtagcc tgaagggttt gggcgaccgc caacgctttc acgtcctcga
    13561 aaccgcgcgc ggcgtaatcc gcttcaaagg ttacttggtt ttccaagccg atggcgcgga
    13621 attgcgcgtt gcgttccacc gtttcgtgat tgatgacgcc gccgcgcctg ccttcgccga
    13681 taccggcgcg ttggttgccg acgttgatat tcgtgacggc tttccagttc gagccgatgg
    13741 tgcggccgcc gcccacgcgc gggatacggt tgcgcaacgg ggtcaatacc ggatagagtt
    13801 tttgagacgg cgcggaaagg tcataggttt gcaggccggc ggtaaagccg gccggctggg
    13861 taaaaccttt gtcaggcggc ccgccgcccg cttgtgccga cttcatcagt tcaatcgttt
    13921 cttgtgtgag ttggttcacg ttcatttatc gctcctgata ataaaaaaac cgcctgtaag
    13981 cggtgttaca gacggcctgt ttgttttgcc ttaatgagtg tcgccacgtc atccagcgtg
    14041 ccgtcattct ttacaatcgg ctgaaaacct tttaacgggt cttcgccgtt atcttctgcc
    14101 ttactgatag ctttcgtact gcctttcggc ggtactgcct gtttcttcag gctttcgatt
    14161 tccgcctgtg ctttggcaag ggcgtcattc gattttttca gcgcgtcttg tgcctttgcc
    14221 agttcgtcag ccgattccgc tttggcaaga tcgcctgatt tgccggcttt ggccgccaaa
    14281 ccgtcggccg gcttatcggc ttcgcttgcc gccaacgctt tcagcgattc ggcaaggccg
    14341 gccgccgatt ctttgatttg cgcggtaacg gcttcatcga tgttgtcgta ggatgcgtcc
    14401 tcaaccagcc atttcagcga catcaataca tcagccggtg atttgacttg gtacattgat
    14461 ttggcggccg tcccgccttt cggcttgtcg gctttagcca ataccgcttt caaggcggcg
    14521 atttcagatt cggacaattc gacgcttgcc gattttccgg cttcgtcctt tttgccggcg
    14581 tccttatcgc ctttatcttc ggttttaggc cctttatcgc cgtctttagg attttcatct
    14641 tcctcttcgg ttggtttgtc agacggcttg ccgtctttat ccgccgcccc tgcctcatct
    14701 ttcggtttgt ccgccttaaa gcaggtaaac accgcgtcag gattggcagg gcggtcgaca
    14761 aggctgattt ccgtcagctt caagcccgtg atttgcgact tgttcaaatc atcgcgggcg
    14821 gtaaccctgc cgccgatgga aaagcctttg taaacgcctg tcttgacttt cgtcacggca
    14881 acagggtcaa cgatatgcgc gccaaaaaat gtgcgcccgt cgtcttccac gttgatttca
    14941 atcgccgttc ccgccgcgtt tgagccgtgc atctcgcgca ccgcgccgaa cttcatataa
    15001 tcgggaatcg ccgccttcat tgcttccgcc gcgacgactt cgccgtccga atcgaccgct
    15061 tcgctcgagg cataccccca aactttgacc gtgccgtcgt cctgcgcctc cgtcttggcg
    15121 atttgcgcgt ataacttcgt cattggtttg ctccaaaaaa aagccgcccc gcgaaggggc
    15181 ggcaaaacac acccgcctga cccgaaggaa tcaagattca ggcatatccc ctgccaaaac
    15241 aggcacaacc gcgcaccggc agttcggatg a
//
LOCUS       DAXDSD010000055        14905 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXDSD010000055 DAXDSD010000000
VERSION     DAXDSD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14905
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.055"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..516
                     /locus_tag="ACFB2Q_001295"
     CDS             <1..516
                     /locus_tag="ACFB2Q_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC0918319.1"
                     /translation="NEFSGDWEQLNKEFEGVISQYQSNDENSNIEIESPEIPKGRERF
                     ARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVASHIKPWGEDKDNRLNPRNGLC
                     LNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLFKPYENRMIRLPERLNPSLEF
                     LKFHRENIFQS"
     gene            607..2970
                     /gene="pheT"
                     /locus_tag="ACFB2Q_001296"
     CDS             607..2970
                     /gene="pheT"
                     /locus_tag="ACFB2Q_001296"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC0918320.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            3044..3346
                     /locus_tag="ACFB2Q_001297"
     CDS             3044..3346
                     /locus_tag="ACFB2Q_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC0918321.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            3330..3638
                     /locus_tag="ACFB2Q_001298"
     CDS             3330..3638
                     /locus_tag="ACFB2Q_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918322.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            3814..4299
                     /locus_tag="ACFB2Q_001299"
     CDS             3814..4299
                     /locus_tag="ACFB2Q_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC0918323.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            4491..4715
                     /locus_tag="ACFB2Q_001300"
     CDS             4491..4715
                     /locus_tag="ACFB2Q_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918324.1"
                     /translation="MSVWDIWDRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEEPCIG
                     RCYAPMPESPDFANAVAMAVAMICIVFIYD"
     gene            4933..6234
                     /gene="bioA"
                     /locus_tag="ACFB2Q_001301"
     CDS             4933..6234
                     /gene="bioA"
                     /locus_tag="ACFB2Q_001301"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC0918325.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRAFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            6231..6878
                     /gene="bioD"
                     /locus_tag="ACFB2Q_001302"
     CDS             6231..6878
                     /gene="bioD"
                     /locus_tag="ACFB2Q_001302"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC0918326.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSHPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            6893..7369
                     /locus_tag="ACFB2Q_001303"
     CDS             6893..7369
                     /locus_tag="ACFB2Q_001303"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC0918327.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            7389..7484
                     /locus_tag="ACFB2Q_001304"
     CDS             7389..7484
                     /locus_tag="ACFB2Q_001304"
                     /inference="COORDINATES: protein motif:HMM:NF026993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH/endonuclease VII fold putative polymorphic
                     toxin"
                     /protein_id="HFC0918328.1"
                     /translation="MRQPASKPHFNIRDSNLDTGHVDGTHGHYNF"
     gene            7493..7900
                     /locus_tag="ACFB2Q_001305"
     CDS             7493..7900
                     /locus_tag="ACFB2Q_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918329.1"
                     /translation="MNIIEIISRNRFLKQIYPSGIMDISLVSFSTDLSNCILTIRTST
                     KPSVEIEKWGLWLKDYDTVEIELRNSFIKGMKCQNWSHNNRNICQVEIKNQEDGLKII
                     RFYDNNSNWLLELEVYGLVFQGCKTYMKEGYES"
     gene            7890..8780
                     /gene="ubiA"
                     /locus_tag="ACFB2Q_001306"
     CDS             7890..8780
                     /gene="ubiA"
                     /locus_tag="ACFB2Q_001306"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC0918330.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            8965..9414
                     /gene="ptsN"
                     /locus_tag="ACFB2Q_001307"
     CDS             8965..9414
                     /gene="ptsN"
                     /locus_tag="ACFB2Q_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC0918331.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            9418..10380
                     /gene="hprK"
                     /locus_tag="ACFB2Q_001308"
     CDS             9418..10380
                     /gene="hprK"
                     /locus_tag="ACFB2Q_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC0918332.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            10361..11215
                     /gene="rapZ"
                     /locus_tag="ACFB2Q_001309"
     CDS             10361..11215
                     /gene="rapZ"
                     /locus_tag="ACFB2Q_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC0918333.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            11296..12237
                     /gene="ylqF"
                     /locus_tag="ACFB2Q_001310"
     CDS             11296..12237
                     /gene="ylqF"
                     /locus_tag="ACFB2Q_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC0918334.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            12338..12940
                     /locus_tag="ACFB2Q_001311"
     CDS             12338..12940
                     /locus_tag="ACFB2Q_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC0918335.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            13124..14797
                     /gene="recN"
                     /locus_tag="ACFB2Q_001312"
     CDS             13124..14797
                     /gene="recN"
                     /locus_tag="ACFB2Q_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC0918336.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
BASE COUNT         3765 a         3508 c         4058 g         3574 t
ORIGIN      
        1 aatgagtttt caggagattg ggagcaactt aataaagaat ttgagggagt catttcccaa
       61 taccaatcaa atgatgagaa tagtaatatt gaaatagaat cacctgagat tccaaaggga
      121 agggaaaggt ttgcaagaat atcagtacgc gtcaatcagg gctttttccg ttccagtgtt
      181 ttggcagcat acaataatca atgttgtatt actggattaa aacaacctga attgttagtt
      241 gctagtcata tcaaaccctg gggggaagat aaggataacc gtttaaaccc aaggaatggg
      301 ctatgtttga atgcgttaca tgacaaagct tttgataggg ggttgttagg aatagatgaa
      361 aatttcaaaa ttatattttc tccactactg gcaaaaacag aaggatttga tgatttattt
      421 aaaccatatg aaaatcggat gataagactg cctgaaagac taaacccaag tctggaattt
      481 ttaaaatttc acagagaaaa tatttttcag agttgagtaa atatcaacat taaccagaat
      541 tctaaaacag actgtctgaa cgaaaattga taaaacaaaa gttaattaat tgattgagaa
      601 cataacatgc aattctccta ctcatggctg aaaacccaag ccaatcctga tctttctgcc
      661 gataaactgg aacatctttt gaccatggcc ggtttggaag tggaagaaat cgatactgcc
      721 gcccctgctt tcagtggcgt ggttgttgcc gaagtaaaat ctgttgaaaa acatcctgat
      781 gcagaccgtt tgaacgttac tcaagttgat gccggtacgg gcgagttggt tcagattgtt
      841 tgtggtgcgc cgaatgtcaa gccgggcatt aaagtgccgt gttcgttgcc ggttgccgtt
      901 ttgccgggca attttaaaat taagccgact aaaatgcgcg gcgtaccatc aaacggtatg
      961 ctgtgttcga ccaatgaact gggtttgcct gatgatggtg tagacggcct gcacattctg
     1021 cctgaagatg cgcctgtcgg taccaacatc cgcgaatact tggatttgga cgatatgctg
     1081 tttacgttga aaattacgcc taaccgcgct gattgcttga gtgttaaggg cattgcgcgt
     1141 gaggtttctg cgttgactca atgtgcgttt acgcctgttg aaattcagac ggcccctatc
     1201 ggcagtgaga aaaaacaggc tgtccgtatt gatgcaccgg ctgactgcgg ccgttttatc
     1261 agccgcgtta ttgaaaatgt caacgcgaaa gcggctactc ctgattggat gaaacaacgt
     1321 ttggaacgca gcggtatccg cagcatttcc gcattggtgg acattggcaa ctatgtgatg
     1381 ctggaaatcg gtcagcctat gcatgttttc gatgctgata agctgtcggg cagtttgatt
     1441 gtccgccgtg ctcaaaatgg cgagactttg gcgtgtctga atgagaagac ggttactttg
     1501 gctgacaata cactggtggt cgctgatgaa aaaggtgcgt tgagcttggc cggcttgatg
     1561 ggtggcgcgg cgagcgcggt ttcagacggc acgcaaaata tcgtgctgga agcggcttgg
     1621 tttgagcccg aaatcatcgt cggcaaatcg cgccaatacg gtttcggttc ggattcgtcg
     1681 ttccgcttcg agcgcggcgt ggattaccgt ttgcaggcgg atgccattga acgcgccacc
     1741 gaattggttt tgcagatttg cggcggtgcg gcaggcgaga tggtggaagc acaaggtaaa
     1801 ttgcccgaag ccaagcaggt cggattgcgt ttgggtcgtc tgaaaaccgt gttgggcgtg
     1861 gacattcctg ccgaacaggt ggaaaccatt ttgcaacact tgggtctgca gcctgagaaa
     1921 acggcggaag gcttccgcat taccgcgccg agcttccgtt tcgacatcga aattgaggct
     1981 gatttgattg aagaaatcgg acgcgtttac ggctatgaaa acatccccga cgattacacg
     2041 tcaggccgtc tgaaaatgtc ggaactgccc gaaacacgcc gtccgcgttt tgccgtttac
     2101 aacgaaatgg cggcgcgcgg ctaccgcgaa gtgatcagct atgccttcgt tgacgagcag
     2161 tgggaactcg attttgccgc caacgccgcc cccatccgcc tgcaaaaccc gctggcggcg
     2221 caatatgccg tgatgcgttc tacgttgatt ggcggcttag tggaaatttt gcaaaacaac
     2281 ctgaaccgca agcaaaaccg cgtgcgcgtg tttgaaatcg cctgcgtgtt cggcaaaggt
     2341 tcagacggcc ggtttgtcca aaacgaacgc atcggcggtc tgtggtacgg cgcggcgatg
     2401 cccgaacaat ggggtgagaa aacgcgcaat gcggattttt acgacatcaa ggcggacgtg
     2461 gaaaatctgt tgaaaaacaa agcggtcgag ttcgttaaaa ccggacatcc cgccctgcat
     2521 cccggacgtg ccgccaatat cgtttcagac ggcaaagtca tcggctttgt cggcgaactg
     2581 catccgaaat ggctgcaaaa atacgacctg ccgcaagcgc cgctggtatt tgaaatcgat
     2641 atggcggctg tgttggaatg cgggaaaacg cgctatcggg ccgtatcgaa attccagccg
     2701 gtgcgccgcg atttggcgtt tgtgatgccg gaagttatga atcatgatga tttactgctt
     2761 gtcttgaaag gcgcagcaaa caagttggta caggaaatca gcgtgtttga cgtgtatcgc
     2821 ggcacgggtc tgcccgaagg gatgaagagc gtggcggtta aagtgatttt gcaggatatg
     2881 gaaaacacgc tgacggatga ggcggtcgag ccgcttattg gaaaactgat tggcgtggca
     2941 acggaggcag gggcgcggct tcgcagttaa aaaataaata ttcgcttgaa ttttaaataa
     3001 aaattggtaa taatccacaa ctgttacaac agaaggtaat catatgacac taactaaagc
     3061 agaactggcc gatattttgg tggataaagt cagcaacgtc accaaaaacg atgccaaaga
     3121 aattgtcgaa ctcttttttg aagaaatccg cagcactttg gcaagcggcg aagaaatcaa
     3181 aatttccggt ttcggaaatt tccagttgcg cgacaagccg caacgcccgg gccgtaatcc
     3241 gaaaaccggc gaggaagtgc cgattaccgc ccgccgcgtg gtaactttcc atgccagcca
     3301 gaaactcaaa ggcatggtgg aacactacta tgacaaacaa cgttgatttg acgattcctg
     3361 caaaacgcta tttcacgctg gacgagttgt gcggactgtt gcaaatcagc ccctatggtt
     3421 ttgcgcaatg gcaacatgat cacggtgtgg ttgtcggtta cggcggagaa cgctacaccc
     3481 gtttggatgt ggtgaaactg ttgaaactga agagcacgtt tgcaccgtat gcagaaggtg
     3541 cggaatcggg ttcggacggc aaccgtccgg ttacgcttca ggaaatcgga gacggtctga
     3601 aagacctgtt ggaggatttg gataaggagt tgtgctgatt tgagaccggt tgcaggtatg
     3661 cagccggttt tgttttacac gctaaaaata attattgcgt gatttcacat tgtttcggct
     3721 tgaagcacat ggttttgtaa tcatttacag gcagctcgct tggaatcctg ttcgggcggt
     3781 ttgctgttta cttaaatata aggatgacgg tcaatgagat ttttcggtat cggttttttg
     3841 gtgctgctgt ttttggaaat tatgtcgatt gtgtgggttg ccgattggct gggcggcggt
     3901 tggacgctgt ttctaatggc ggcaaccttt gccgccggtg tgctgatgct caggcatacg
     3961 gggctgtccg gtcttttatt ggctggcgcg gcggtaaaaa gtagtgggaa ggtatctgtt
     4021 tatcagatgt tgtggcctat ccgttatacg gtggcggcgg tgtgtctgat gagtccggga
     4081 ttcgtatcct cggtgttggc ggtattgctg ctgctgccgt ttaagggagg ggcagtgttg
     4141 caggcaggag gtgcggaaaa ttttttcaac atgaaccaat cgggcagaaa agagggattt
     4201 ttccacgatg acgatattat cgagggagaa tatacggttg aagagcctta cggcggcaat
     4261 cgttcccgaa acgccatcga acacaaaaaa gacgaataaa tatgaatgga atgccgtctg
     4321 aaggttcaga cggcattttt ccggtttgaa aatatagtgg attaacaaaa accagtacgg
     4381 caaggcgagg caacgccgta ctggtttttg ttaatccact ataaatttaa tccactataa
     4441 aatagggctg taaccttcaa tcggaatttg ttgcctgcgg gatatacggt atgagtgttt
     4501 gggatatatg ggacaggatg gtggaaatct atcataagta taagaagccg tgcctggttt
     4561 tagcagtgga ttttgtgatg ggtatggtat tcatagagcc gaatgaggag ccgtgcatcg
     4621 gcagatgcta tgcgcctatg ccggagtccc ctgattttgc caacgctgtt gcgatggctg
     4681 ttgctatgat ctgtatcgta tttatctatg attgattgaa gcgttgggcg gaggctgtgt
     4741 gaaacggtat tgggcgttgg gtcgtctgat tccaatcagg cttgaggaat gcgaaacggt
     4801 gtgcgcttat gttgcggacg atttgtttcg cggtttttcg cccgaaacgg atggaaaggt
     4861 gtgggaagcg gtctgtcgga gtagaatacg ctttttgcgt ttgaatgcag taataagaaa
     4921 agagagaaac ttatgccgtc tgaacatcaa cacacatcat cattacttaa tttcgaccgt
     4981 acccatctgc tccatcccta tacttccatg accgatccgc ttcccgttta tcctgtcaaa
     5041 cgtgcagaag gggtgtttat cgaattggcg gacggcacgc ggctgattga cgggatgtcg
     5101 tcttggtggt gtgcgataca cggttacaat catcccgttt tgaatcaggc ggttgagaat
     5161 cagatgaaac aaatggcgca cgtgatgttc ggcggcctga cgcacgagcc ggcggtggag
     5221 ctgggcaagt tgttggtcgg gattttgccg caggggctgg accgtatttt ttatgcggat
     5281 tcgggttcgg tttcggtaga agtcgcgttg aaaatggcgg tgcaatacca gcaggcgcgg
     5341 ggtctgacgg cgaagcagaa tattgcgacg gtacggcgcg ggtatcacgg cgatacttgg
     5401 aacgcgatgt ccgtttgtga tccggaaacg gggatgcacc atattttcgg cagcgcgttg
     5461 ccgcagcgtt attttgtcga taatccgaaa aaccgtttcg acgatgaatg ggacggggcg
     5521 gatttgcagc ctgtccgcgc cttgtttgaa gcgcatcatg tggatattgc cgcctttatt
     5581 ttagaaccgg tcgtgcaagg cgcgggcggc atgtattttt atcatccgca gtatcttcgc
     5641 ggcttgcgcg atttgtgcga cgaatttgat atcgtgttga tttttgacga aatcgccacc
     5701 ggattcgggc gcacgggcaa gatgtttgcc tgcgaacacg cggaggtcgt gccggatatt
     5761 atgtgtatcg gcaagggttt gagcggcggc tatatgacgc tggcggcggc aatcacttcg
     5821 caaaaagtta ccgaaacgat ttcacgcggc gaagcgggcg tgtttatgca cggcccgaca
     5881 tttatggcga acccgctggc gtgtgccgtt gcctgtgctt cggtcaagct gcttttatcc
     5941 caagactggc aggcaaatat ccgccgtatc gaaagcatct taaaaggccg tctgaaagcc
     6001 gcgtgggaca ttcgcggcgt gaaagacgtg cgcgttttgg gtgctatcgg cgtgatagag
     6061 ttggaaaaag gcgtggatat ggcgcgtttt caagcggatt gtgtggcgca gggcatttgg
     6121 gtacgcgcgt tcggcaggct ggtgtatctg atgccgccct atatcatttc agacggcatt
     6181 ttgaccaaac ttgccgataa aaccgtgcaa atcttgaagg aacacagcaa atgaaaggcg
     6241 tttacttcgt cagcggtata gacacggaca tcggcaaaac cgtcgccacc ggtatgttgg
     6301 caaaacaatt gttgcagcag ggcaaaagcg tgattacgca aaagcccgtg caaaccggtt
     6361 gccaagacat cgccgaagac atcgccgtcc accgtaaaat catgggcata cccatgcagg
     6421 aagctgatga acagcgtctg actatgcctg aaatcttcag ccatcccgcc tcgccccacc
     6481 ttgccgcccg actggacggc aggggtttgg acttggacaa aatccgcacc gccacacaag
     6541 aattggcggc gcagtacgaa gtcgttttgg tcgaaggcgc gggcgggttg atggtaccac
     6601 tgacggaaaa gctgttaacc attgatcata ttcagcaaca ggcttatccc gtcatccttg
     6661 ttaccagcgg acggctggga agcattaatc acaccctgct cagtttcgtc gtgctcaaac
     6721 aatacggcat tcgcctgcac agcctcgtgt ttaaccacat ccacgacagc cgcgacgcac
     6781 acgtcgccca agacagcctg aactatctgc aatgccgtct gaaagccgat tttcccgaag
     6841 cggaatggat ggagttggca aaaacaggcg cggtataaag attgggaaag atatggaaca
     6901 cctatttggg aaatggctgc ccgacttgcc cgcccccgtt tcagacggca tcgacctgcc
     6961 gatgagccgg ttattgaaag cccggtcgct gaccgccgca ttgtgcgcct tgccgcatac
     7021 attttcggtg gaactgctga aactgggcga agcggaaacg gaatacggac ggcggagggt
     7081 gcgcgatgtg cttctgaaac tggacggcac tgctgttgtg caggcacgaa gcgcgtgcag
     7141 cgtcggttcg gcgttttggc aaaacatttt ggactgcggc acacgccctt tgggcgagcg
     7201 tctgtttcaa gccgatttgg aaggggcgcg ttcggcgttt gagtttgccg tttccagtga
     7261 aggatgcgga cggtactttg ccgcgcggcg ttctcggttt tcccatcaag gcgaggaaat
     7321 gctgctgacc gagtattttc tgcccgaact gaaacgcttt atcggataaa ataccgtttt
     7381 ttcaagctgt gcggcaacct gcatctaaac cacattttaa tataagagac tccaatttag
     7441 atacaggaca tgtcgatggt actcacggac actataattt ttagtaagaa atatgaatat
     7501 aatagaaata ataagtagga atcgttttct aaaacaaata tatcctagtg gcataatgga
     7561 tatttcacta gtctcttttt caactgactt gtctaattgt attttaacta tccgaacaag
     7621 tacaaagcct tctgtagaaa tcgaaaaatg ggggctgtgg ctaaaagatt atgatacagt
     7681 tgaaattgaa ttaagaaata gctttattaa aggaatgaaa tgtcaaaatt ggtcgcataa
     7741 caatagaaat atatgccaag tagaaataaa gaaccaagaa gatggtctaa aaataataag
     7801 attttacgac aataattcaa attggttatt ggaactagaa gtttatggat tagttttcca
     7861 agggtgtaag acttatatga aagagggtta tgaatcttaa atccccttta tttttacgac
     7921 tgtccgatcg tttggacgtt tatatcagac tgatgagggc ggacaagccc attgggacgc
     7981 tgcttttact gtggccgacc tactgggcat tgtggctggc ttcagacggc attcccgatt
     8041 tggcggtatt ggcggcgttt acaatcggca cgtttttaat gcgcagtgcc ggctgcgtca
     8101 tcaacgactt tgccgaccgc gattttgacg gtgctgtcga gcgcaccaaa aaccgtccgt
     8161 tcgcacaggg cagggtcaag aaaaaagaag ccctgctgct gacggcattt ttgtgtctgc
     8221 ttgccgcatt gtgcctgatt ccgctgaatc atctgacatg gctgatgagc ctgcccgcgc
     8281 tgttccttgc gctgacttac ccgtttacca aacgcttttt tccgattccc caattttatc
     8341 tgggacttgc cttttccttc ggcatcccga tggcgtttgc cgcagtgggc aacagcgttc
     8401 ccgttgaagc gtggatactc tttgccgcca atgttttatg gacactggct tacgataccg
     8461 tctatgcaat ggcggacaaa gaggacgatt tgaaaatcgg catcaaaacc tccgccgtca
     8521 cgttcgggcg ttacgacatc gccgccgtta tgctgtgtca tggcggcttt accctgctga
     8581 tggcagtatt gggtgcggtt atcggtgcgg catgggcata ttggacggca atccccatcg
     8641 tcctgctgct gcaataccgc caatatgccg ccatcaaaag ccgcgtccgg caaatctgtt
     8701 ttgaaacgtt tttggcaaac aacagaatcg gttgggtgtg gtttgccgct atttttgccc
     8761 atacgttttt cgcgaaataa ggcagggcaa tgccgtctga agaaccgtaa actgctttgg
     8821 acggcatttc tatctgtgcc gaaaagcgtt aaaatatgtt tttgaaacgc tgtgttatgt
     8881 cagcccgtac cgtatgcggg attgaggttt gccccggcag tcggtacaat ccttctgttt
     8941 tgcgatgtct gaaaagagaa gcttatgagc cttatcggcg aaattttgcc tttgtcccat
     9001 attgttttgg atatggaggt gggcagtaaa aaaaggctgt ttgaggaagc gggcctgctt
     9061 ttggaacgcg aatcctcatt gtcccatgct gatgttttcg aatgtctttt tgcccgtgaa
     9121 aaactcggtt cgaccggttt ggggcagggt gtcgccatcc cccacggccg ccacgcgagc
     9181 gtgaagcagg cgacgggcgc gttcatccgt acgcgcgaac ccgtcggatt cgacgcgcct
     9241 gacggcaaac cggtttcact ggtttttatc ttgctggtgc cggaaaacgc aacgggcgag
     9301 catttggaag tcttatccaa actggccggc aagttttccc aaaaaagcat cagagaatcg
     9361 ctgatgacgg ttgcttctgc ggaagaagcc cgcgccatcc tgaccgaaga ataaaatatg
     9421 cccagtatct ccgtccgccg cctgtttgac gacaaccaat acaagttgca acttgcctgg
     9481 gccgccggca attcgggtgc ggacaaccgt atcggcgtag aggcggacaa gcccgtcctc
     9541 gccctggtcg gacacctgaa tttcatccat cccaaccaaa tccaagtggt cggtttggcg
     9601 gagtcggaat atctgaaccg cctcgaatcg ggggagacgg gttatcagtt cggagacctg
     9661 ttcgatattt ccatgtcttt ggtcattgtg gcaaacgatt tgccggtttc cccggggctg
     9721 cgcgactact gccacaaaaa cgacatcccc ctgttgactt ccaaactcga aagcccttat
     9781 ctgatggatg tgttgcggat ttacctgcaa cgcaccttgg cagcatcgtc cgtcaaacac
     9841 ggcgtatttc tcgatgtgtt tgaaatcggc gtgctgatta ccggacattc tggtttgggt
     9901 aagagcgaat tggcattgga actgatttcg cgcggccata gcctgattgc cgacgatgcg
     9961 gtcgagctgt tccgcatcgg tccggaaaca ttggaagggc gttgttcgcc tatgctgcgg
    10021 gattttttgg aagtgcgcgg cttggggata ctcaatatcc gccatatttt cggcgaaact
    10081 tccatccgcc ccaaaaaaat cctgcaactc attatcaatt tggtcgaggc ggacgacgag
    10141 tatatgaagc agctcgaccg gttgagcatc cgcaccgaaa ccgaatccat cctcaacgtc
    10201 aacgtccgtt cggttacgct gcccgtcgcc gtcggacgca accttgccgt tttggttgag
    10261 gcggcggtac gcaattacat tttgcagttg cgcggcaaag acagtacgcg cgaatttttg
    10321 gaacgccatc agacgcaact taaagaaaac gaacagaatc atgaaaatcg tcctgattag
    10381 cgggctttcc ggctcgggca aatccgttgc actgcgccaa atggaagatt tgggctattt
    10441 ttgcgtggac aacctgccct tggaaatgct gccgtcgctg gtgtcgtacc atatcgaacg
    10501 tgcggacgaa accgaattgg cggtcagcgt cgatgtgcgt tccggcatag acattgccca
    10561 agcgcgcgag cagattgcct atctgcgcgg actcggacac agggtggaag ttttgtttgt
    10621 cgaggcggaa gaggccgtgc tggtccgccg cttttccgaa acccggcgcg gacatccttt
    10681 gagcaatcag gatatgacgc tgttggaaag cctgaaaaaa gagcgcgaat ggctgttccc
    10741 gcttaaggaa atcgcctact gtatcgacac ttccaagatg aatgcccaac agctccgcca
    10801 tgcagtccgg cagtggctga aggttgagcg taccggactg ctggtggttt tggagtcctt
    10861 cgggttcaaa tacggtgtgc cgaacaacgc cgattttatg ttcgatatgc gcagtctgcc
    10921 caacccgtat tacgatcccg agttgaggcc ttacaccggt atggacaagc ccgtttggga
    10981 ttatttggac ggacagccgt tggcgcagga aatggttgac ggcatcgaaa ggtttgttac
    11041 gcgttggttg ccgcgtttgg aggatgaaag caggagctac gttaccgtcg ccatcggctg
    11101 taccggcggg cagcatcgtt cggtctatat tgtcgaaaaa ctcgcccgaa ggttgaaagg
    11161 gcgttatgaa ttgctgatac ggcacaggca ggcgcaaaac ctgtcgggcc gctaatcccg
    11221 tcaaaccatt atgccgtctg aaaccccgtc cgccgtttca gacggcattt caaatattga
    11281 gaagaaagaa cagacatggc aatccaatgg tttcccggcc atatgaacaa ggcaagaaaa
    11341 gctatcgccg agcgtgccaa aagcgttgat atggtgattg aaatgctgga cgcgcgtatg
    11401 cccgcctcca gcgaaaatcc actgttggcg cagctttcca aaggtaagcc caaactcaaa
    11461 attttaaaca agcaagacct tgccgaccct gaacgcacaa aggtctggct tgaccactac
    11521 aacagccgcc ccgacacccg ggccatcgcc ctcgattcct ccgaaaccgg cgcacacggc
    11581 aaaatcaccc aagcctgccg cgcgatgatt ccccaccggc aaggcataga caaacccctg
    11641 cgcgttttga tttgcggcat tcccaacgtc ggcaaatcca ccctcatcaa tggaatgata
    11701 ggcaaaaaat ccgccaaaac cggcaacgaa cccggcatta ccaaagccga acaacgcctt
    11761 ttccttgccg acgacttctg gctgtacgac accccgggaa tgctgtggcc gaaaatcatc
    11821 gtcgaagaaa gcggctacaa cctcgccgcc ggcggcgcag tcggacgcaa cgcattggac
    11881 gaagaagaag tcgccctcga acttttagac tacctccgcc gccactacct ccctatgctg
    11941 caagaacgct accaagccga caaagacccc agcagccact gggacgacaa ctcgtggctc
    12001 gaatggatag ccaaaaaacg cggcgcagtc ctcagcggcg gacgggtcaa ctaccaaaaa
    12061 gccgccgaaa acatcctcac ggacttccgc gaaggcaaaa tcggcagaat cacccttgaa
    12121 acaccgaacc aatgggaaac ttggcttaaa aaagcacgtc agaaagaagc cgaactcaaa
    12181 gccattcgcg aagccagaaa agcagagcgc aaagggcaga agccttcgga agcataaaga
    12241 atgccgtctg aaaaatattt ttcaggcagc ttctctctac tccaaccgat ttcagacggc
    12301 atatccaaac ccatgccgtt tcagcacgga tacccgtatg accgacaaaa tttctcccga
    12361 cgcgctgatt gaagccgcac tgctgaccca aaccgaaccg ctgaccgaaa aatctatgcg
    12421 cgaactgtgt gtgccgccgt tgtcgcaaga caaactgatt gatgtgttgg cgcagttgaa
    12481 aacgcgttgg caggataggg cgttgcaact ggtgcatacg caggagggct ggcgttttca
    12541 gattgttcag acggcattcg agcggctggg cagcctgcaa gaacagcgtg cgccgcgcta
    12601 ctcccgcgcc gtgatggaaa cactggcgat tatcgcctac cagcagcccg taacgcgcgg
    12661 cgacatcgaa ggtatacgcg gcgtggcggt gtcgcagaac gtgatgcaga ccttgcagga
    12721 tcgggggtgg attgaagtca tcggacatcg ggacacattg ggaaaacccg cattgtgggc
    12781 aacaacggca acttttctca gcgatttgcg cttggacggt ttggaagaac tgccgccgct
    12841 gaccgaactg ggcgaactgg ttttgcccga tttgatagaa atgccgccta cggatgaaga
    12901 agagccggaa accgtaccgt ccgataccct gcccaactga aattccaaat gccgtctgaa
    12961 acgcacattg cttcagacgg cattgcaaca aataagcaga taaaaacaaa cactaagcaa
    13021 aattaaggaa aaacttattt ttaatttaaa aaatcttaga tataattcgt atatctaaag
    13081 ttgatattgc ttttgtcggt agaattgcta aggaatcctc acaatgcttc taacactttc
    13141 tttgcgtgat tttgtcattg tcgaaaatct gaatctggat tttcaaagcg gttttaccgt
    13201 tttgaccggc gagaccggcg cgggcaagtc cattactttg gatgcgattg gtctgctgtt
    13261 gggcgataag gccgattaca gccaagtccg cagcggcgca aaagaagcgc agttgtcggc
    13321 attgttcgat atttcccatt tgcctgcttt aaaagcagaa ttgcgtgaac aagggctgtt
    13381 ggatgagggc ggggaagaac tcagtatccg ccgcattatc gatgccaaag gcaaaagccg
    13441 cagttttatc aacaatcagg cggctacctt ggcgcaactc aaagccgtcg gcgggcagct
    13501 tatcgacatc cacgggcaaa acgaccatca ttcgcttaat caggaagctg cccagcgcga
    13561 attgttggac gcatttgtgg gcggcagggt gcaggcggaa accgtcaggc agctttatca
    13621 aaactgggcc aatgcgaaaa aagccctcca agaggcgcag gaacacgccg atgccgtcat
    13681 tatcgagcgg gagcgtctgg aatggcagtt taacgaactg aatcagttgg acattaaaca
    13741 aggcgaatgg gaagctctca gccaaagcca cgacagcctt gcccattctg ccgaattgtt
    13801 gcaggccgcc gaagaagtcg gaagcaagat tgacggcgac aacggcatcc aacgccatat
    13861 ctatcaatgt caaaaactat tggccaatct gcaaaacatc gagccgcgct ttgccgagag
    13921 cctgaatatg ttggcaagca tcgaggccga attgggcgaa atcagtgcca atatgcgcga
    13981 tgtggcagga cacagtgaca tcaaccccaa tgaattggcg gcacaggaac agcgtatggg
    14041 cgaactgatg gggatggcgc ggaaataccg gattgaaccc gaagagttgc ctgccaagtt
    14101 ggcagaaatc gaagaacgcc tgcaaagtct tcaagctgcc gctgatttgg acgcgctcga
    14161 gcataatgtt gcccacaatt ttgccgaata tcaggaagct gcccacatcc tttctgccat
    14221 gcgccatcag gcggcagggc gtttgagtag cgaaacgacc gagcatatgc aacaccttgc
    14281 catgaaaggc gcgcgtttcg acatcgtcct gttgccttcg tcgccgacgg cacacggttt
    14341 ggagcaggtt caatttcaag ttgccgctaa caaaggcaat ccgccccgcc cgctgaacaa
    14401 agtcgcctcc ggcggcgaat tggcgcgtat cagccttgcc ttacaggttg ttgccagcca
    14461 atatacccaa gttcccaccc tgatttttga tgaggtcgat accggtattg gagggggagt
    14521 ggctgaaatg gtcggcaagg cattacgtgc gttgggcaga aaacatcagg tgcttgccgt
    14581 tacccatctt ccgcaagtcg catcctgcgg agaaaaccac tggcgggtgc gcaagcacag
    14641 cgagggagag caaaccgtca gcgaaatcag tatattggat gaaatacaac ggatcgaaga
    14701 ggttgcccgt atgttgggcg gagaagttat taccgatacg acgcggcaac atgcggcaga
    14761 attgctgcaa cttgcgtcga aaaatagttt attttaaaat caatcagtta caaaaataac
    14821 tgaaaataga agtctaaaat aatagacagg actcagataa atccgtatta tcacgctttc
    14881 ttaatcactt gaacaagtga ttgtg
//
LOCUS       DAXDSD010000056        14699 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXDSD010000056 DAXDSD010000000
VERSION     DAXDSD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14699
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.056"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(323..4492)
                     /locus_tag="ACFB2Q_001313"
     CDS             complement(323..4492)
                     /locus_tag="ACFB2Q_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC0918337.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     TVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPDTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AEHVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVSSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFWHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(4547..6394)
                     /locus_tag="ACFB2Q_001314"
     CDS             complement(4547..6394)
                     /locus_tag="ACFB2Q_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC0918338.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYQGNYWTSNVSYNRSTTQNIEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6766..7995
                     /gene="sstT"
                     /locus_tag="ACFB2Q_001315"
     CDS             6766..7995
                     /gene="sstT"
                     /locus_tag="ACFB2Q_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC0918339.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8146..9426
                     /locus_tag="ACFB2Q_001316"
     CDS             8146..9426
                     /locus_tag="ACFB2Q_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC0918340.1"
                     /translation="MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     NAGEGVLPKEKKDEEAAGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKN
                     EDAGAQNDMPQNAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVID
                     GPSQNITLTHCKGDPCNGDNLLDEEAPPKSEFESLSDEEKIKKYKKDGEKFTGLVAIK
                     VENNGLNKYTIIYQAQPTRSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLVGTAVYNGEVLHFHMENGRPYP
                     SGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKDRD"
     gene            9426..9629
                     /locus_tag="ACFB2Q_001317"
     CDS             9426..9629
                     /locus_tag="ACFB2Q_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC0918341.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            9689..9937
                     /locus_tag="ACFB2Q_001318"
     CDS             9689..9937
                     /locus_tag="ACFB2Q_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918342.1"
                     /translation="MFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKGIAFPNRGGAT
                     GCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(9930..10145)
                     /locus_tag="ACFB2Q_001319"
     CDS             complement(9930..10145)
                     /locus_tag="ACFB2Q_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918343.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(10138..10353)
                     /locus_tag="ACFB2Q_001320"
     CDS             complement(10138..10353)
                     /locus_tag="ACFB2Q_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC0918344.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10556..11899)
                     /gene="argG"
                     /locus_tag="ACFB2Q_001321"
     CDS             complement(10556..11899)
                     /gene="argG"
                     /locus_tag="ACFB2Q_001321"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC0918345.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            12024..12824
                     /locus_tag="ACFB2Q_001322"
     CDS             12024..12824
                     /locus_tag="ACFB2Q_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC0918346.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12864..13850
                     /locus_tag="ACFB2Q_001323"
     CDS             12864..13850
                     /locus_tag="ACFB2Q_001323"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC0918347.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
     gene            14511..14699
                     /locus_tag="ACFB2Q_001324"
                     /pseudo
     CDS             14511..14699
                     /locus_tag="ACFB2Q_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         3088 a         3754 c         4440 g         3417 t
ORIGIN      
        1 tttcttaacc atcccttcca atagccgtgc gaaggttttt ttatatcagt gggaaatgca
       61 atattctatt gtttttattg tagaatttta aaaacagatt gttgtgttcc gcgtttttgc
      121 cggtttggaa gcggtggggc gcatcagcct tttataaagg catatcggaa gcctgtataa
      181 ggtttttgaa catatcgatc ctgttccctt gcaggcggac ggcagcggcg gatggcgaaa
      241 ggcgggtttt caaatgtccg gtttggcggc gcgtctttca gacggcatgg cggcggagcg
      301 tgccgcgccg tctgaaaacc gtttatttcc ctttgccgtt tccggcggag tcttttttgt
      361 ccgaaccgaa gaggcggtcg aaacgtatgg tgtatgtcag ctcgccgccc gacgaacggc
      421 tgccgatacg ggcaaccgcc tgtatggcgc gggtcagccg gtaaatcagt ttgacggact
      481 gttccgcgct ggagatgccg tattcgtagc cgatgtagag tttgccggtc agttgtttgc
      541 cgacggtcag cacctgttcg gcggggttga gttcgccggt ttgcgcgttg cggctgcgct
      601 tgctggtaaa gcccaaatca tccaccagcc cgatgcggtc gttgatttgc ccggcaagca
      661 gcgcgcctgc ggctgcggac agggcggcat tgtcgccgct gctgccgctg ccggcgcggt
      721 tgaggatgag ccaggagagc ttgtcttttt cactcatcgg ttcgtttgcc gtcagcgtaa
      781 tgcgcgggct gttgaggctg cccaatattt ccacgcccgc accgacgggg gaaaggcggc
      841 gttcggcgcg gatgttgagg ttggggtcgt tgagcgggcc gacaaaggag actgtgcctt
      901 tggtaatgtc taaatcctgc ccgtatgctt tgtaacgccc tttgatgacg cggaccgtgc
      961 ccaccccacg cacatttccg cccggttgcg cggtcagggt cagtttgccg cctatggtaa
     1021 cgtccgcgcc gtagccggag aagcggatgc cgtcattgag gtctaaagtc aggttcatat
     1081 tgacggggag cgatgccgcc gcctctttct tgacttcgcc caatacgacg acatcgtcgc
     1141 cgacggacgg catcgaggat ttttgcgaac cgaacagccc ctgatcagtt ttaatcatac
     1201 cggtaacgga tatgcctttt tgcggcgaat agcgcaggcg ggtgttgccg gaaaccgtca
     1261 ggcggcggtt ggggcgggac aggatgcggt atttgtcgaa caccgcgccg atatcgacat
     1321 cgggcacgct gttttccatg ctgaccgtgc cggagagttc cgccgtccct tcgtgccaga
     1381 atttcaggct gtcgattacc catttcctgc ctgcaatatg cgaacgcagc gagccgttgt
     1441 ccaagatgat gccttgggtt tggttgcggt aatagagctt gtcgccgttg atactgccgc
     1501 ccaagtgcgg gtcggcgatg ctgccgccga gggttacggc ggcattcagg ctgcctttga
     1561 cggtttgtcc gaccggtagg aagttgcgga atacttcggc atcggcaacg gtcaggttga
     1621 gcctgccgcc caaaggtgcg gtatcgaagg agcggctttg cccgacggtg atattgccgt
     1681 tgattttccc gtagttgctg ctaccgttga cggcggcatt gacggacgga gagcttaccc
     1741 gtccgccgat ttgcgcggag gcattcaggc tgccggtaat gttttgcgcg gcggcgggca
     1801 gaaagggctt caatgcgccc aagtcgggaa gggaggctgt aatcctgccg ccgagcggtg
     1861 tatttgccat attgccgccg aaggcgttgc cgatgcccaa atcggcgtta atccgtccga
     1921 aacgcgcgcc gccgtcaagc aggattccga tgcggtcgtt ttgaaagcgc gttttcaggg
     1981 aaaatgcgtt caaacccaaa gcctgcccgc cgggcaatac ggcatcgccg ctttgccggc
     2041 tgatattgag gtagccgcgc gcgttgtgcc cgtaggcgac atcccagtcg ccgtttaaaa
     2101 ccagattgtg ttcgaagggc ggtttgaaga aattgtgcaa ctcggcgatg tgcaggccgc
     2161 gtgcgccgcc ttttgccgat atgccggttt tcctgtccca agaaaagtgt tgcaggttga
     2221 ggctgccgcc cattgcctgc caatttgccg cacttgccgc cacgtgttcc gcaccggctt
     2281 cgagcgtcat acggttttgc agcttgaggt tgaatgcgcc gccgatgtcg aggatgccga
     2341 tgctgccttt ccatcgggta agttccctgt tgatgccgcc tgaagcgtcc aaatcgagtt
     2401 tgaacggttt gccgtccagc gtcatggcgg cgtgtgtgcg gatgcggtgc tgcgcgcccg
     2461 taccttccag cgtcaggccg gcggtatcga caaccgccgc gccgcccgac agggaaaggc
     2521 ggccgccctt gatatcggcg cgcatcgggc ggcttgtgtc gggcgagcct ttgagggtaa
     2581 aatcgagcga acggatgtct gccgctttgc cgatgtgtaa gttgcgcgcc gtgccggaaa
     2641 ggtcggtttc aaaggttcgg atgccgccgt ccaaatcgcc ggaaaggtgt ccgcgtacat
     2701 ttaaagaccc cgcgagtccg aaaccgaaac gggataaatc gggtgcggtg atattgaggt
     2761 taagccggtc gccttttttg ccgaagccgc cgtctgtttt gacgatgttc cgccccaacc
     2821 gcaaatcgac ggcggcacgc ggaaggtggc gggactcgta aacaatgtcg gcactgccgg
     2881 caatcggcac gccgttgaac gtaccgggca aaaaacgcat tttgcccgta aatttctctt
     2941 ttgccagttc accggcaaga tgaatcgaac cgttgatatt gcctgccgga aattgcggat
     3001 cgatgcgtga agggtcgaat gcgcgggaac ggatgtccag cttgagcagg cggtctttaa
     3061 acagctcgag atagccttgc gcggtcaggc tgccttcccc ggcggagatg ttgacggtgt
     3121 cgaacaccag tttccgctgt tcgtttgcgg ggtcgcttgc gatggcgagg ctgccgtccg
     3181 tgcgtgccgt gccggtgccg agttgccaag agattttggg cgaggcggtc gtgccgccga
     3241 tgccgatgct gccgtccaac ctgcctttga acgcggtttg cagcacgtct tccgcgccga
     3301 cggagttgat gcctatattt aaatcaagga tgtctttttc ggtgtcgatt ttgcccgaca
     3361 gcctgatgcc gccccgtccg agcagggcgg cggacgtatt gccgatatgc accgtgccgt
     3421 cctgccggat gacaaagccg cccaaaacct gacggacggg gatgccgttg cggtcggcaa
     3481 agccggcttt ggtgttttcc aaatcgagcg agccttccag cgcgatgccg tctgaaaacg
     3541 acgggatggc ggtcaggtcg aaattcagcc cggcatcggg cagggaaggc acgaaggcgg
     3601 acggattgat gttgaatcct ttgaccagta cttcttccaa tgttttatcc aatgattcgg
     3661 caaacgggtg gatgacggat tttcccgaga ggcggatatt gccgccgtcg atcgtcagtt
     3721 cggcgcgcac atccttcagg ctgccgctca gccgcgccgt actgtgtatg gtttcgcctt
     3781 cgaatccgcc tttggtgtaa atggcggtat cgagggcaaa cggttttttc aagccgaccg
     3841 aggctgaccc cgacgaactg ctccacggcg tgtcggcggc cttcaggtcg aggcggtgcc
     3901 ctttacggtc gtaacggtat gccgcgttga ggcgttcgag atagacagtt tgtttgtcaa
     3961 aggttttgcc catgctgatt ttgcccgtct cgaagcggtc gagatagacg gtggcgggca
     4021 ggtctatgct gtcgggcagg ccttgaggcg ggcgttcttc tttaggcgga gtcggtttgg
     4081 ttacgatggc gatgtcgccg gcggagatgt cggtgatgtg caggctgcgg cgcatcagtt
     4141 cggacggttt ccacgcgaag cggaagcggc tgattttaag gtctgccccc tcggtttcta
     4201 tcgaccagtt gtcgccgtcg aagccgtcga gcagtgtgcc tttgaggttt tgggaggaaa
     4261 tgtttacgcc gaaccaggac gggatttggt acagcccgaa gcgcaaacct gcttccgtac
     4321 cggcgatcca gccgaggaaa catactgcca aaatcaggac agacagcagt gccgccgaca
     4381 gcttcagcag cgggcggcgt ttttttgccg gcggggcggg gcggtgttca gacggcattt
     4441 tgcgcgtgcc gttttcggtc ggatcggtat ctgtcggtgc ggtatcggtc ataatgtctg
     4501 tttcaaaatg gtttgctgca atgccgtctg aagcggccgt atcggtttag aaacgcgttc
     4561 ccaagctgat gtgccagcgg atttttttgt cgctgtgtcc gtaggcgatg tcgaaggaaa
     4621 acggcgcgag cgggctgaac cagcgcacgc ccagtcccga accgtgtttc agcttcatac
     4681 gtttgaaatt ggcggcggca tcgcccatat cgtggaatac cgcgccggac agggtgcgcg
     4741 taaacggcag ttggtattcc aagctgccca ccaagagggc gcgttcgggc aggaccgatc
     4801 cgttcggacc ggcaagcccg atgctgtcaa gttcgtaacc gcgcacggaa gacgcgccgc
     4861 cgctgcggaa catcagcccc gaggggacat cggcattgtc gcgtgcaacg gtgtaacccg
     4921 cttgtccgcg tatgatgaac gtgccgagtt ttttgttttc gggcgtgaag aaataacctg
     4981 cgcgggcaga ggtgcggatt agcgcggtgg aggacaggaa tgtgcccaaa gtcgtcccga
     5041 ttttgccgtc gaggtaatgg ccgttttcgg ggtgcagcac gttgttgagc agctggcgtt
     5101 tccaagaggc ggtcagcatc gtggcgtggc tgttgcccaa atcgacatcc gagccgggga
     5161 ttttccggcc ttctgcgaga aattccgccc ccagcctggc atcgatgccc gcgcggtcgc
     5221 gcacatacca gatgccgccg gagaaggcgc gtttttcaat gttttgggtg gtcgaacggt
     5281 tgtaggaaac gttgcttgtc cagtagttgc cctgatagtt gcgcggctgg ctgatgccgg
     5341 cggcaagcgt ggtttcgtat ttgtccatat cccagacgac cgagccgata tagcctttgt
     5401 tgaagaggtt gtaatagtcg taggcgattt tgccgcccaa accgtattcc gaatcgaggc
     5461 ggatgccggt ttcgagtttg tggcgtttga cctcggttac gctgactttg acggggacgc
     5521 ggtcgccttg gaggcggtcg aagtcggctt gtacggacgc gccggaataa tgcccgtttt
     5581 gttcgagcgc ctgttggaag tcgagcagca ggtcgaggtc gtagggcgtg cccggttgga
     5641 agcgcgccag gccggagacg gtttgttcgg ggtaacgctg tgtgccggtg atttcaaagt
     5701 cgccgaaggc gatggggcgg ccgctgtcca cgacgacgtt caaatcggcg gtggcggtat
     5761 cggggttgac ggccgcccgg gtgttgccga gcttggcaag cgggtagcct ttgcgcgtta
     5821 ccgcgccgag gacggaagtt ttgctgtttt cccaactgtc ctgatcgaaa tcgctgccta
     5881 ccggctgctg ccagttttcc agcgcgttgc ggtagtattc ggcgaggttg ccgtctgaaa
     5941 ggatgtcgcc gaggatggcg acgccgacgt tggcgatttt ggtgcgcggg cccggtgtga
     6001 tgtgcaccgt ataagctccg tctttttccg tcaggctgac cttgctgctg aaatagcctt
     6061 tgctgcggag catggttttg acgttgtccg gtgcttcttc ggcaaggaat cccgtctgtt
     6121 ccttatccaa aacctcttcc tgctgctgcg tgatgagcgg caggtgttct tcgaccatat
     6181 ctttgatttc actgtcctgc gtgtcgatgc ggacggggaa tttgggtttt aatttgactg
     6241 attcggtgtc ggggcttttg cttttgaaca atgcgaaacc cgccgccttg ttttcggaaa
     6301 ggtcggcggc aggcgcgtat gcgtgcggaa agaaaaataa agccggcagg agcagggcgg
     6361 tcggtttgat catcatggta cgggtgtcgt gcatcggtgt ggcggatttc gattctaaca
     6421 ctgttcggac ggggcgggac gcttcagacg gcatactgcc gacggtattt cggaggttgc
     6481 ggcccgccgg aaatgtatct gcttgtttta aggcattttg tttccaatcc gcatatggaa
     6541 aacaggctgc gtttcggaat agtgtcgaaa aataggcagg attaaataaa tttattctcg
     6601 ttcaataaat taaaccgttg cgccgaatga taaaacaaag cctgtccctg catagggcaa
     6661 atcaacgcac ctcaaaacac agcttgaaaa ataaaaaaaa cacagagaat gggttttctt
     6721 ttaaaataca acatttttta acatatcaca acataaggaa gaattatggc gttcggcaaa
     6781 tccttatttc atgcgatcgg ccgcgtcagc ctggtcaggc agattgccgc cggtttggcg
     6841 ttgggcatcg taatcggttc ggtttcaccg caactgggct tggcagcagg cttgttcggc
     6901 agcctgtttg tcggcgcgct caaagcggtc gcgccggttt tggtatttat tttggtggcg
     6961 gccacaatcg cgcagcacca aaaaggcaac aaggcgcata tcaggccgat tatcgtcctt
     7021 tacctcatcg gcacgttttc cgcagccctg accgccgtca tcgccggtat ggttttcccg
     7081 acgcacattg ttttggcggg cgcgggcgat gtgtccgccg cgccgccttc cggcattgtg
     7141 gaagtgttga aatcgctgct gatgaacctg gtcgccaacc cgattaacgc gattgccaat
     7201 gccaactata tcggcatttt ggcttgggct ttggttttgg gcgcggcgtt gcggaatcac
     7261 ggttcggacg ttacgcggca ggtcgttgcc gatttggcgg aagcggtttc caccgtcgtg
     7321 aaatggatta tccgttttgc ccctttgggc attttcgggc tggtgtcgtc cacaatcgcg
     7381 gagacgggtt tcggcgcgct ggcgggttat gcgaagctgc ttgcggtgct gttgggctgt
     7441 atggcgttta tcgcgctggc ggtcaatccc gccatcgtgt ggtggaaaat ccgccgcaac
     7501 ccttatccgc tggtgtttac ctgcctgcgc gaaagcggcg tgtatgcctt ctttacccgt
     7561 tcttccgccg ccaatatccc cgtgaatatg gctttggcga aaaagctggg gctgcacgaa
     7621 gacacttatt cgatttccat cccgttgggg gcgaccgtca atatgggcgg cgcggcgatt
     7681 acgattaccg ttttggctat ggcggcggcg cacacccaag gcatacaggt tgattttgcc
     7741 accgcgctgc tgttgagcct ggtggcgacg gtcagcgcgt gcggcgcgtc cggcgtggcc
     7801 ggcggttcgc tgctgctgat tccgctggcg tgcagcctgt tcggcatcag caacgatgtc
     7861 gccatgcagg tggtcgccgt cggcttcatc atcggcgtga ttcaggattc ggcggaaacg
     7921 gcgttgaatt cttcaaccga cgttttgttt accgctgccg ccgatttggg ccgacagaga
     7981 aaccgtgcgg aataaaccaa gctatgccgt ctgaagcccg tttggcgttc agacggcata
     8041 tttttacaat tttatgggca tatggcggaa acaataccgg ctgccttgaa tatgtttcaa
     8101 cacacaggtc gatacataaa gcgtcgccct atgaaggaga tgatgatgtt taaacgcagt
     8161 gtgattgcaa tggcttgtat ttttcccctt tcagcctgtg ggggcggcgg tggcggatcg
     8221 cccgatgtca agtcggcgga cacgccgtca aaaccggccg cccccgttgt tgctgaaaat
     8281 gccggggaag gggtgctgcc gaaagaaaag aaagatgagg aggcagcggg cggtgcgccg
     8341 caagccgata cgcaggacgc aaccgccgga gaaggcagcc aagatatggc ggcagtttcg
     8401 gcagaaaata caggcaatgg cggtgcggca acaacggaca accccaaaaa tgaagacgcg
     8461 ggggcgcaaa atgatatgcc gcaaaatgcc gccgaatccg caaatcaaac agggaacaac
     8521 caacccgccg gttcttcaga ttccgccccc gcgtcaaacc ctgcccctgc gaatggcggt
     8581 agcgattttg gaaggacgaa cgtgggcaat tctgttgtga ttgacggacc gtcgcaaaat
     8641 ataacgttga cccactgtaa aggcgatcct tgtaatggtg ataatttatt ggatgaagaa
     8701 gcaccgccaa aatcagaatt tgaatcgtta agtgatgaag agaaaattaa gaaatataaa
     8761 aaagatggag aaaagtttac tggtttggtt gctataaaag ttgaaaataa tgggttgaat
     8821 aaatatacca ttatttatca agctcagcct actcgttctg cacggtcgag gaggtcgctt
     8881 ccggccgaga ttccgctgat tcccgtcaat caggccgata cgctgattgt ggatggggaa
     8941 gcggtcagcc tgacggggca ttccggcaat atcttcgcgc ccgaagggaa ttaccggtat
     9001 ctgacttacg gggcggaaaa attgcccggc ggatcgtatg ccctccgtgt gcaaggcgaa
     9061 ccggcaaaag gcgaaatgct tgttggcacg gccgtgtaca acggcgaagt gctgcatttc
     9121 catatggaaa acggccgtcc gtacccgtcc ggaggcaggt ttgccgcaaa agtcgatttc
     9181 ggcagcaaat ctgtggacgg cattatcgac agcggcgatg atttgcatat gggtacgcaa
     9241 aaattcaaag ccgccatcga tggaaacggc tttaagggga cttggacgga aaatggcggc
     9301 ggggatgttt ccggaaggtt ttacggcccg gccggcgagg aagtggcggg aaaatacagc
     9361 tatcgcccga cagatgctga aaagggcgga ttcggcgtgt ttgccggcaa aaaagatcgg
     9421 gattgatgtt gatgccgccc gaagcgcagg tattcattac ggcgggacga cgcgttcaaa
     9481 tcatcaaaca agcccccgaa aaaggcggca tcccaaaatc cggcactgaa attgccgcgc
     9541 gtatcggcgg cattccgcag cctgccgggg aaactgtcgg cagcagccgg aatgtcgggt
     9601 atttccgccc gcctttttcg ggctgttgaa atatgaaaat aaagcatagg ccgtctgaat
     9661 gttcagacgg cctgtttttt taacggctgt gtttcaacac tccgccggtt ccaaccggca
     9721 gcagcaggga gataatgcct tgaaccccgc tcttcggggc ggcagggttc gggccggttt
     9781 ggggaaaggc atcgcctttc cgaatcgggg cggtgcaacg gggtgcgcct tatgtgccga
     9841 aagggtttgc ggcaacggta tgccgtctga agcgggtgcg ttcagacggc attgtgatgt
     9901 ttggggcgcg ggtccggttc aaatccgtat cagttgataa acccgttgtc tttcattttc
     9961 ttttcggaaa aatactggcc gcataccagc agggcggcga ggatgatgac aaacagccct
    10021 gccatagtca gtgtgatttt gaagaacaat tcggcttcga gaaaagaaaa ccaaagttgc
    10081 acaatcagca gcagcaccca gccggcaaac agtccgagac aggcataaac gccgagctta
    10141 aacacggtcc cgctccaaaa tgacgacgta ggcttcgcgt ttgaaaaaca gcagcatacg
    10201 gcgcaaatct ttttccatcg ttacgacccg ccagccttcg ggggtttgtt tgttgaggaa
    10261 cgcgctgaaa ttgacggggt tgacctttgc cgcgccgaaa aacaggctgg acaacaggct
    10321 ttcctgataa atgacgactt tgtattcttt catcgtattt ccttttcggt tgaaaccccg
    10381 ccctttgggg cggtaggatc agactttatt tgggagggaa ccccttccaa atcaggacga
    10441 tacatagggc ggtgctttat gtatcgtcct gtgtgttgaa acataggatt cctttaatca
    10501 agggattgcc ttttcaggca atcccttaaa ttgcagcgat gcaatgcggc gaaatttatt
    10561 gcttattgcc caactgcggc aataccgagc cttcgcccag cgagagcaaa ccgctttgcg
    10621 aataaatacc cagtttggcg cgggtgtcgg tgatgtcgag gttgcgcatc gtgagctgtc
    10681 cgatgcggtc gagcggtgtg aacgccgcac cttcgacttt ttccatactc aggcgttcgg
    10741 gttggtaggt caggttgggc gattcggtgt tcagaatcga gtagtcgttg ccgcgccgca
    10801 gttcgagggt aacttcgccg gtaacggctt tggcgaccca gcgttgggcg gtttcgcgca
    10861 acatcaaggc ttggctgtcg aaccagcggc cttggtagag caaacgtccg aggcgcaggc
    10921 cgttgatgcg gtattgttcg atggtgtctt cgttgtggat gccggtaacc aagcgttcgt
    10981 aggcgatgtg gaacaatgcc atgcccgggg cttcgtagat gccgcgcgat ttggcttcga
    11041 tgatgcggtt ttcgatttgg tcgctcatac ccaagccgtg gcggccgccg atgcggttgg
    11101 cttcgaggaa gagttcgacg gggtcggcgt attctttgcc gttcaatgca accggcacgc
    11161 cttcttcaaa gcgcacgctg acttcttcgg gttcaatttc gacgttttcg tcccagaagg
    11221 caacgcccat gatgggctta acgattttga tgcccgagtt caaaaattcc aagtctttgg
    11281 cttcgtgggt cgcgccgagc atattggaat cggtggagta ggctttttca accgacattt
    11341 tgtagttgaa gccgttggca atcagaaatt cgctcatttc gtgacggccg ccgagttcgt
    11401 cgataaattg ctggtcgagc cagggtttgt agattttcag cgcgggattg gtgagcaagc
    11461 cgtagcggta gaaacgctcg atgtcgttgc ctttgtaggt gctgccgtcg ccccagatat
    11521 tcacatcgtc ttctttcatg gcggaaacga gcatggtgcc ggtcacggcg cggcccagag
    11581 gcgtggtgtt gaaataggcg atgccgccgg tggaaacgtg gaacgcgccg cattggatgg
    11641 cggcgatgcc ttcgtgtgcc agttgtgcgc ggcagtcgat caggcgggcg ttttccgcac
    11701 cgtattccat tgcttttttg ggaatggcgt tgtaatcgtc ttcgtcgggc tggccaaggt
    11761 tggcagtgta ggcataaggc agcgcgcctt tgagtttcat ccacaacagc gcggcagagg
    11821 tatcaagacc gccggagaag gcgatgccga ctttctgacc gacggggagg ttttgtaaaa
    11881 tagtatggtt ttggttcatg aaaaaatcct cgatagcttg aaaaatcaaa gccattatct
    11941 tataaataga aaatattgtc agccatcatg ccgtctgaac gggtattccg ccgtttcagg
    12001 cggtatttaa aaccgaaaac attatgaacg cgttcaacct catcatcatc ggagacgaaa
    12061 tcttacacgg cagccgccaa gacaaacatt tcgccttttt taaatccctg ctcgaatcca
    12121 aagggctgaa gctcaatcag gtgcaatatc tgcccgacga acccgatttg ctggtcaggc
    12181 aactgcgccg cagcttttca gacggcatac cgacgttcgt taccggcggc atcggcgcca
    12241 cgcccgacga ccgcacgcgc caaaccgcag cggctgcttt ggatttgccc gtcgtccgcc
    12301 atcctgaagc cgccaagttt atcgaaggca taacccggaa acgcggcaag ccgctcgatt
    12361 cgcccgaaca cgcccaacgc ctgaaaatgg cggatttccc cgagggcgca gagcttgtgc
    12421 ccaacccgtt caacaacatc gccggatttt ccatccgcga gcattatttc ttccccggct
    12481 ttcctgtgat ggcgcacccg atggcggaat gggttttgga aacttattac gccggccgct
    12541 tcaaccaaac cgaacgcggc agccgcagcg tgtatgtgtt tgagcagccc gaatcgcgca
    12601 ttacgccgat tatagagcat atcgagcaaa cttatcccgg cgtgcgttcg tacagcctgc
    12661 ccagcgtcgg ttggacgcat tcagacggca cgcaggtcaa accgcacatt gagttcggca
    12721 tcaaagccga aggagaagcc gtcaaccttt tggacgcggc gtgggcggaa gtgttgcaca
    12781 gcttggacgg attgggagca gagctgaaaa atcgggtaaa ctgaaaactg tttgcccaaa
    12841 tcggacgata tttaaggaaa accatgcaat accgaatcag aagagaaaac gaagcacccg
    12901 aagcaaaaaa tgcgggggaa accttatggg agcgcgacat tatgcgcgaa gtgctgctgt
    12961 ccgcctatcg ggacaggcgc agggagcgga tgtggaaaaa catctggcgc gccgtcagca
    13021 ccctgattct ggttgccctg attgcaggca ttttccgaaa agacgaagcc gcattgcagt
    13081 tggcgggcaa cacgccgcat accgcagtcg tcaacctgta cggcaaaatc ggcaacggcg
    13141 tagaagacca ggtcaaaaaa ctcaaagacg ggatggaggc cgcctacaaa aacccgcagg
    13201 caaaagccat cgtcatccgc gccaacagcc ccggcggttc gcccgtcgtc tccaacaccg
    13261 cttttgaaga aatacgccgt ctgaaggcgc agcaccccgg catccccgtt tatctcgtgg
    13321 cagaggatat gtgcgcgtcc ggctgctact acatcgcggc ggcggcggac aaaatctatg
    13381 ccgacccgtc cagcatcgtc ggcagcatcg gcgttatcgg cagcagtttc gacgcgaccg
    13441 gcctgatgga aaaaatcggc gtaaaacgca gggttaaaat tgcgggcagc aacaaaggca
    13501 tgggcgatcc gttttcgccc gaaacgcccg aacagtcgaa aatctgggaa gaaatgctga
    13561 ccggcataca cggcgaattt atcaaagccg tcaaaaccgg acgcggcggc cgcctcaaat
    13621 tccgacaata tcccgacgtg ttcagcggtc gcgtgtacac aggcgcggac gcgctgaaag
    13681 tcgggctggt ggacggactc ggcaatattt acagcgtggc gcgagacgtg gtcaaagctc
    13741 ccgatgtggt ggattacact ccgaaggacg atttcggcag aatcttgggc agacgcttcg
    13801 gagcagaact caaagcaagc gtttcggaag ctctgcaggc cgtccggtag cgttgtttac
    13861 agtatttcta agggaatacc agaataaaaa tatacacaaa aacactcacc tccccagtat
    13921 gctgccacta agtcagccgc gttactattt accgtgcact taaagccgca aaaggcaggt
    13981 tgtccccggg aggttttgtg catcctgata ccgatcggtt gctgttgctg ttgctgttgc
    14041 tcaaaggacg gaaggccgcc gataaacgag attacctgtt tgtcgctatt gacgattttt
    14101 atactctgcc attttgccgg acaaaaccgc agacagtgct gccaagtttc tgaccgaaca
    14161 tctggctgac ccctgcttgt acctggttga gtacgtttac tctgacaatg ataggtaata
    14221 taaagagccg tccaacatgc tttcggtgca gcttgttatg ataatgggat tggttggagg
    14281 cttgcccgat ttgcttgtcc gcagaccaac ggtaaggcgg agcgggttat ccgtaccttg
    14341 atggagatgt ggcatgagga acagtcgttt gacagtccgg aacatcagca aaaggagttg
    14401 tgctgcttta ttaatttagg cgctgtccta gataactagg gaaattcaaa ttaagttaga
    14461 attatcccta tgagaaaagc cgtctaagcc ggtataaaca aaataaactc attgggctat
    14521 ttgtcgcagg tgtaactgca agaacagcgg cagagttggt aggcgttaat aaaaatactg
    14581 cagcctatga ttttcatcgt ttacgatgac tgatttatca aaacggtccg catttggaaa
    14641 tgtttgatgg cgaagtagaa gcagatgaaa gttattttgg cggacaacgc aaaggtaaa
//
LOCUS       DAXDSD010000057        14271 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXDSD010000057 DAXDSD010000000
VERSION     DAXDSD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.057"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(57..1157)
                     /locus_tag="ACFB2Q_001325"
     CDS             complement(57..1157)
                     /locus_tag="ACFB2Q_001325"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC0918348.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRVVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1482..3056
                     /locus_tag="ACFB2Q_001326"
     CDS             1482..3056
                     /locus_tag="ACFB2Q_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC0918349.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            3070..3258
                     /locus_tag="ACFB2Q_001327"
     CDS             3070..3258
                     /locus_tag="ACFB2Q_001327"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918350.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(3296..4717)
                     /gene="hemN"
                     /locus_tag="ACFB2Q_001328"
     CDS             complement(3296..4717)
                     /gene="hemN"
                     /locus_tag="ACFB2Q_001328"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC0918351.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIK
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPGDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            4852..5586
                     /gene="fnr"
                     /locus_tag="ACFB2Q_001329"
     CDS             4852..5586
                     /gene="fnr"
                     /locus_tag="ACFB2Q_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC0918352.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(5699..6649)
                     /locus_tag="ACFB2Q_001330"
     CDS             complement(5699..6649)
                     /locus_tag="ACFB2Q_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC0918353.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            7013..7723
                     /gene="rmpM"
                     /locus_tag="ACFB2Q_001331"
     CDS             7013..7723
                     /gene="rmpM"
                     /locus_tag="ACFB2Q_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC0918354.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPARNHHQH"
     gene            8498..9454
                     /gene="thiL"
                     /locus_tag="ACFB2Q_001332"
     CDS             8498..9454
                     /gene="thiL"
                     /locus_tag="ACFB2Q_001332"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC0918355.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            9447..9932
                     /locus_tag="ACFB2Q_001333"
     CDS             9447..9932
                     /locus_tag="ACFB2Q_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC0918356.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGAFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(9992..11668)
                     /gene="ettA"
                     /locus_tag="ACFB2Q_001334"
     CDS             complement(9992..11668)
                     /gene="ettA"
                     /locus_tag="ACFB2Q_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC0918357.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGDIKIGYLPQEPELDPEKTVREEVESGFGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            11904..13258
                     /locus_tag="ACFB2Q_001335"
                     /pseudo
     CDS             11904..13258
                     /locus_tag="ACFB2Q_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
     gene            13262..14223
                     /locus_tag="ACFB2Q_001336"
                     /pseudo
     CDS             13262..14223
                     /locus_tag="ACFB2Q_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
BASE COUNT         2987 a         3596 c         4051 g         3637 t
ORIGIN      
        1 aaaggggtgg aaatgccgtc tgaacggcgg aaatggcttt cagacggtat ttgcgctcaa
       61 taataatatc ccgcgcccag aatacacggt ttggatgcgc cggtcgcttt gtgcggacta
      121 ccgggaatgc ggttaatcca acacgccgcc aaccacgcaa atgcggccgc ctccacccat
      181 tgaggatcga ggttcagttc ggcggtgctg tgcagggaaa cgcgtgtgcc gaaacattct
      241 gccaaatccg ccattaaaac aggattgcgg atgccgccgc cgcaaatgta catttgacgg
      301 gcatctgccg ctgcgtgtga gacggcgtcg caaacggttt gcgcggtgaa tcgggaaagc
      361 gtccgcaata cgtcgtatcg gttttcgccg ccgtcaaggt aggtttcgag ccaatttagg
      421 gcaaacagtt cgcgccccgt gctttttggg tggggttgtg agaaatacgg gtgggcgagc
      481 agcctgccga gcagttgcgg caatatgttg ccttgtgccg cctttgcacc gtttttgtcg
      541 taaggcagct gccagtgtgc ctgcgtccac gcgtccatca gcatattgcc cggccctgtg
      601 tcgaagccga aggcgggtgc gccggggggg agtacgctga tgttggcaat cccgccgatg
      661 ttcagtacca cgcgtgtttc cctgtcatcg cggaacaggg cttcgtgaaa ggcggggacg
      721 agcggcgcac cttgtccgcc ggcagcaagg tcgcggctgc ggaagtcgcc gacggtaaaa
      781 atccgcgtca gttccgccag cagcggcaaa tcggcaagct gtatgctgta accgtgttcc
      841 ggcgcgtgtc ggacggtttg cccgtggcag ccgagggcgg taatgtcgca cggagcgagg
      901 ttttgactgc acagcagttc ggcggcggtt tgcgcgtaca ggcggctgag ttcttgcgac
      961 aacatcctgc tgcggtgcag ttcgtctgtg cctgtgtcct gcaaatccag caatttgcgg
     1021 cgcaaccggt cagggtaggg ggtaaaggcg tgcccttccg cgcccagcca tttgccgccg
     1081 tccatccgta ccagcacggc atccgccccg tccatactgg ttcccgacat aatgccgatg
     1141 taaagctgtg tttccatcat cactcccaaa ctggtgcaaa acgccatttt aacgtgtatt
     1201 gacgctcgta taccgatttg ccgccgcagt gtaaataaag tgtaaataaa tgttttaaga
     1261 cgggtggaaa aatattataa tgcgcccgca acatccagta gtagaagtgt catacaaacc
     1321 gtttccggca gcagttttgc attcggtcag gtttgggggt attcggatgc ggttcagaag
     1381 gaaggatgcg tctgccatat cccgaaacgg cagttcgacc ggaggcagca atacagtgtc
     1441 ggcaacactc atgatttcca ccacattaaa ggaagattgc catggctcaa atccaaatga
     1501 gcgcaaatgt taaaaccatc aacgccgtct ttgccgccat gctggtaggt acagtcggct
     1561 attttattta ttggggcttg ggttataccc attacaatta cgccgcctta ttcattattg
     1621 ccacgatgtt cggtgtgttt atggcgttca acatcggcgg caacgatgtt gccaattctt
     1681 tcggcaccag cgtcggtgca ggcacgctga ccatcccgca ggctttgctg attgcggcgg
     1741 tatttgaggt cagcggcgcg gtcatcgcgg gcggcgaggt aaccgatacc atacgcaaag
     1801 gcatcgtcga tttgaagggt gttgatttcg aacccataca gtttgtgttt attatgatgt
     1861 ccgcgctttt ggcggcggcg ttgtggctgc tgttcgcgtc ccgcaaaggc ctgccggttt
     1921 cgacgacgca cgcgattatc ggcggcatcg tcggcagtgc gttgtgtatg gcgtttacgc
     1981 ataatgcgga cggggtggcg ttgatacggt ggggcaagct gggcgaaatc gggatgtctt
     2041 gggtattgtc gcccgtgttg ggcggcgcgg tgtcctattt cctgttttcg cgcgtcaaga
     2101 aaaacgtctt agattacaac gcttgggcgg aaggaacgct caagggcatc aagcaggaaa
     2161 aaaaggccta taaagaacgg caccgcctgt ttttcgaggg tttgtccgaa gccgaaaaag
     2221 tcgagtacgc caccaaaatg gcgcacgacg cgcaaattta cgacgaaccc gaattcgatc
     2281 ctcaagagct gcaatcggag tattaccgcg gtctttatgc gttcgacaac cgtaaaaaca
     2341 atgtcgattc ctacaaggcg ctgcattctt ggattccctt tatcgcttcg ttcggcacga
     2401 tgatgatttc cgctatgctg attttcaagg gcttgaaaaa cctgcatttg gggatgagca
     2461 acgtcaacag cttcctgacc atctttatga taggcgcggc ggtgtggatg gggacgtttg
     2521 tgtttgccaa aagcctcaag cgtaaagact tgggcaaatc gacctttcag atgttttcat
     2581 ggatgcaggt ctttaccgcc tgcggcttcg cattcagcca cggtgcgaac gatatcgcca
     2641 acgccatcgg cccgtttgcc gcgattatgg atgttttgcg gacgaacagc gttgccgcgc
     2701 aaagcgccgt ccctccgatt gcgatgctga ctttcggcat cgcgctgatt gtcggtttgt
     2761 ggtttgtcgg taaagaggtg attaaaaccg tcggtacgag tttggcggaa atgcatcctg
     2821 cttcgggttt taccgccgaa ctgtccgccg catccgtcgt gatgggcgcg tcgctgatgg
     2881 ggctgcccgt gtccagtacg catatcttgg tcggcgcggt actcggtatc ggtctggtca
     2941 accgcaatgc caactggaaa ctgatgaaac ccatcggttt ggcgtgggtc attaccctgc
     3001 ctgccgccgc tgtattgtcg atggccagct atttgatttt gcaggcagtg ttctgattgt
     3061 aaaatactga tgccgtctga acccgtgttc agacggcatt ttgttgatgg aatgtgcggg
     3121 cttgtgcctt atgcacaatc tgttctgtcg ggatatgctg tttggtatct tggtttcgga
     3181 acggtcggac acaaaggtta tgatatgccg ccgcctgttc ttgaaaacac ttatcctcgg
     3241 cagcaaaatg ccgtctgaaa aagcctttca gacggctttt gtacgttagc cgcaatcaca
     3301 ccgtttgcga atatttcgct ttggtttctt tgtggcgcag gtggtagtcg aagaccatgg
     3361 cgatgttgcg gatgaggaag cgtcctttcg gtgtaacggt cagcccgtgg ctgttcaggc
     3421 gcaccaatcc caaaccggcg agtttttcca aatccgccag ttcgtctttg aagtagcggt
     3481 cgaacgggat gccgaacata ctttcgtaaa tcctgtagtc gagcgcgaag cggcacatta
     3541 aatcctgaat gatgttgcgg cgcaggatgt cgtcctgatt gagctggtag ccgcgcatga
     3601 tgggcagtct gccttcgtcg atggcggcat agtaggcatc gatgtcgcgt tcgttttggg
     3661 aataggtgct gccgattttg ccgatggacg acacgccgat ggcgaccaaa tcgcaatccg
     3721 cgtaggtcga atagccttgg aagttgcgtt ggaggaagcc ttctttgagg gcgatggaga
     3781 gttcgtcgcc aggtttggcg aaatggtcca tgccgatgaa gacgtagccg cgctctgtca
     3841 gggtttggac gcagtattgc agcatatcga gcttctcttc gctgtcgggg acggcggcgg
     3901 tatcgatgcg gcgttgcggt ttgaacacgt gcggcaggtg ggcgtagtgg taaagggcga
     3961 ggcggtcggg atcgagcgac aaaacggtat ctatggtggt tttgatgctt tcggaagtct
     4021 gatgcggcaa gccgtaaatc aaatcgacgc tgacggattt gaaccctgcc tcacgggcgg
     4081 catcgatgac ttctttggtt tcttcgtagc tttggatgcg gttgactgcc gcctgcactt
     4141 tggggtcgaa atcctgaatg ccgacgctca tgcggttgaa gccgagtctg ccgagcatga
     4201 ggacggtgtc gcggctgact ttgcgcgggt cgatttcgat ggaatattcg ccggacggta
     4261 tcagttcgaa atgtttgcgg atcatgcgga agacacgttc gatctgttcg tcgctcaaaa
     4321 aggtcggcgt gccgccgccg aagtgcagtt gggcaagctg gtgccgtccg ttcagatgtg
     4381 gagcgagcag ttccatttct ttttcaagat atttgatgta ggtatcggcg cggcttttgt
     4441 ctttggtgat gattttgttg cagccgcagt agtagcagat ggtgttgcaa aacggaatgt
     4501 gaatgtaaag ggaaagcggt ttgtttaacg cgcccatacc gcgcaaatgt aaaactttga
     4561 tatattcgcc ttcgcggaaa ccgtcatgga aacggtcggc ggtagggtag gaagtatagc
     4621 gcgggccgct ggcgggcagg ctggcaatca gcgcgcggtc aaactcgggg cggtcatcgt
     4681 ttacattgtg attgttctgt atctgaatga ttttcatggt gtgtgtgcgg ttttatgatg
     4741 ttagtcaaat tttggatagt ttggtagaat gccacagtat gataaacctg tcttgatatg
     4801 tgtcaacaag cacataatcg ggatggggca aaacacacga aaaggtacgg tatggcttcg
     4861 cataatacta cacatcagat gaaaacgctg tgttcttcct gttctttgcg ggaactctgc
     4921 ctgcctgtcg ggctgctgcc caacgagttc agccaactcg atgccgtcat ccgccaaagc
     4981 cgccgcctga aaaaaggcga atacctgttc cgtgccggcg gagcctttac ctcgctcttt
     5041 gccatccgtt cgggcttctt caaaacaacc gtcgccagtc aggacggccg cgatcaggta
     5101 acgggtttct ttatgtcggg cgaactcatc ggcatggacg gcatctgttc ctatgtgcac
     5161 agttgcgacg cggtcgcctt ggaaaacagc gaggtatgcg agttgccgtt tacccatatc
     5221 gaagagctcg gtcagaatat ccccagcctg cgtacacact ttttccgcat gataagccgc
     5281 gaaattgtgc gcgatcaagg tgttatgctg ttgttgggca atatgcgcgc cgaagagcgg
     5341 attgccgcct tcctgttgaa cctttcccaa cgcctttatt cccgaggttt cgccgccaac
     5401 gacttcatct taagaatgtc ccgcgaagaa atcggcagtt atctcgggct gaaacttgaa
     5461 accgtcagcc gcacattatc caagttccat caggaaggat tgatttccgt cgaacacaaa
     5521 cacatcaaga ttctcaatct gcaggtgctg aaaaaaatgg tatccggttg ttcgcacgcc
     5581 atttgattaa cctgtacgaa catttcagac ggcattctca ataaacacag ggcagacgaa
     5641 aacatctgcc ctgtttgttg tatctgccgc aaagtgccgc ctgaaaaccg gcagccgcct
     5701 aaatcgaaaa atcctcgctg atgggcgtgt acagaatcct atccaccttc tcgcgtgtca
     5761 ggtgcggcgc aaacgcttgg ataaagtcgt aggcatatcc gcgcaaataa gtatcgctgc
     5821 gcaaagctat ccacgtcggc gacggctcga acaggtgtgc cgcatccaca agctgcaaat
     5881 cgccgtccgt atccgggttg tatgccattt tcgccatcag tcccacgccc aaacccaagc
     5941 gcacataagt cttcaataca tccgtatccg ccgccgccaa tgcgacatcc ggccgttcca
     6001 aacgggcttt ggcgaatgcc cgcgcgatgc tgctgcccgc attgaatgca aattcataag
     6061 taatcagcgg aaacctcgcc aaatcttcaa tacggagggg attcctgcat tcgagcagcg
     6121 gatggtcgtt cggcacaatc accgcgtgtg tccagtcgta gcaggaaagc cttcccaact
     6181 cggggtggtc gtctatccgt tccgtaacaa tcgccaaatc agattctccc gaagtaacca
     6241 tttgggcgat ggcggcaggg cttccctgtt tgatggtcag attgactttc ggatagcgtt
     6301 tcacaaaatc ggcaacaatc aacggtaggg catagcgcgc ctgcgtatgc gtcgtggcaa
     6361 ccgtcagcga accgctgtcc tgtccggtaa actcgctgcc gatattttta atgttctgta
     6421 catcgcgcaa aatacgttcc gcaatatcca aaaccacctt gcccggctgc gagaccgaaa
     6481 ccacgcgctt gccgctgcgg ataaaaatct gaatgccgat ttcttcttcc agcaatttga
     6541 tttgtttgga gatgccgggt tgtgatgtga ataaggcttc ggccgcttcg gaaacgttca
     6601 ggttgtgccg gtaaacttct aaggcgtatt tcaattgttg taatttcatg gcgggtcggt
     6661 gtgggtctgt gtcgggtggc tgaacattgt ttataattta tcatattttc ttgccggtac
     6721 ggtatggggc tttgccgttg tgtttgttgt ttttgtgcaa cggcaatcgt gcgatatgga
     6781 aaaaatcccc ctaaagtaat gacacggaat tgatttttcg gcatgataga ctatcaggaa
     6841 acaggctgtt ttacggttgt tttcaggcgt tgagtattga cagtccgccc cctgtttctt
     6901 tatagtggag actgaaatat ccgatttgcc gccatgtttc tacagcggcc tgtatgttgg
     6961 caattcagca gttgcttctg tatctgctgt acaaatctaa tgagggaata aaatgaccaa
     7021 acagctgaaa ttaagcgcat tattcgttgc attgctcgct tccggcactg ctgttgcggg
     7081 cgaggcgtcc gttcagggtt acaccgtaag cggccagtcg aacgaaatcg tacgcaacaa
     7141 ctatggagaa tgctggaaaa acgcctactt tgataaagca agccaaggtc gcgtagaatg
     7201 cggcgatgcg gttgccgtcc ccgagcccga acccgcgcct gtcgccgttg tggagcaggc
     7261 tcctcaatat gttgatgaaa ccatttccct gtctgccaaa accctgttcg gtttcgataa
     7321 ggattcattg cgcgccgaag ctcaagacaa cctgaaagta ttggcgcaac gcctgagtcg
     7381 aaccaatgtc caatctgtcc gcgtcgaagg ccataccgac tttatgggtt ctgaaaaata
     7441 caatcaggct ctgtccgaac gccgcgcata cgtagtggca aacaacctgg tcagcaacgg
     7501 cgtacctgct tctagaattt ctgctgtcgg cttgggcgaa tctcaagcgc aaatgactca
     7561 agtttgtcaa gccgaagttg ccaaactggg tgcgaaagcc tctaaagcca aaaaacgtga
     7621 ggctctgatt gcatgtatcg aacctgaccg ccgcgtagat gtgaaaatcc gcagcatcgt
     7681 aacccgtcag gttgtgccgg cacgcaatca tcaccaacac taaggctagg taatatcttg
     7741 ccgatgcatg aggttagcgg attttgtacc gggtactgtt gcaatattcg tgaaacgtcg
     7801 gccggtatcg atgatgtgaa acaaaccccg cttttgcggg gtttgttttt ttgggtggtt
     7861 ttctgaaacg gctatcgtca gaatcggggt gcaggttcgg attcggattc agattcatgt
     7921 ttgtgtccca ttgccgcgct ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
     7981 cgcagacagt acaatagtac ggcaaggcga ggcaacgccg taccggttta aatttaatcc
     8041 actatatcgg ttgaaactct gattttaagg cggtaggatg tgggtttgcc catagcaagg
     8101 gaatcctttc tgtatcaagc cccgaaaggg ataattcata caaattcacg cctttccccc
     8161 tcattgggaa atggatggaa tcgtgcccga tgtgtgcggc actgtatgcc ggatatggtt
     8221 ttatcatcat cccttttcgg ttgaaacccc gccagttgca gcgattgagc ctaatcggtg
     8281 gcggaagttg ccgctttgca ttcggggcgg cgtgcagtgc ggtgctttga tatgccgttt
     8341 gtgtgttgaa acagggtggt cggtgcatac gggtacggta tggctaaagc taaaagtgaa
     8401 atacgctgaa acactgaatg agccgcttta ttgtttgtac gacctttgct gctttgctat
     8461 gatttaaatt ggattcgcac gccggatatt ttgggatatg aaagaattcg acttcatcaa
     8521 acggtatttg caaacaggta cggataagga tgtcgtattg ggcataggcg acgatgcggc
     8581 gattgtccgc ccgcgcgaag gcttcgattt gtgtttcagt gcggatatgc ttttgaagga
     8641 caggcatttt tttgcagatg tcaaacctga aggcttggct tggaaggttt tggccgtcaa
     8701 tatttcagat atggcggcga tgggtgcgat accgcgttgg gtgctgctga gcgcggcgtt
     8761 gcccgaattg gatgaagtat ggctggaacg gttttgcggc agctttttcg gtttggcaaa
     8821 aaagtttggc gtaacgttaa tcggcggcga tacgaccaag ggcgatatgg cgttcaatgt
     8881 aaccattatc ggcgaattgc cgaagggtag ggcgttgcgg cgtgatgcgg cggttgcggg
     8941 cgacgatatt tgggtgtcgg ggcgtgtcgg tatggcggcg gcggctttga actgccgtct
     9001 gaaacggtgt gtgttgccgg atgatgtgtt cgccgaatgc gaacaaaagc tgctccgtcc
     9061 cgagccaagg gttgggctgg ggcttgcgct gttgccgttt gcccgggcgg cacaggatgt
     9121 ttcagacggc cttgcgcaag atttggggca tatcctgacc gcttccggcg tgggggcgga
     9181 aatttgggcc gattcgctgc catctttatc tgtattgaaa gatattttgc cccgagcgca
     9241 atggctgtct tatactttgg cgggcggcga cgattacgag ctggttttta ccgctccgga
     9301 aagttgccgc agccgcgtgc ttgatgcggc ggaacggtgc ggcgtgccgg taacgtgcat
     9361 cggcaaaatc aacggaggat gccgtctgaa ggttttagat gccggcggca gggaattgga
     9421 actacattct ttaggattcg atcattttgg ctgattttaa acctgacttt gcgtggctgt
     9481 tgaaacggcc gttgtgtttt ttggctttcg gtttcggcag cgggctggct ccgtttgcgc
     9541 cgggcgcatt cggcactttg gcggcactgc ctttggcgtt tgtgctgatt ttgctcggca
     9601 tagacgggct gctgctggct tttgtgtgta tcgtgctgtt tatgtggggc atacgtattt
     9661 gtgcgtgtgc ggaacgtgaa acgggtgtca gtgaccacgg tgggattgtt tgggacgaga
     9721 ttgtcgccat gctgtttgtg ctggcgtttg tgccgttcag gtggacgtgg tggctggcgg
     9781 catttgttct gttccgtctg tttgatgcgc tcaaaccgcc ccccgtcggt tggtttgaca
     9841 agaatctgca cggcggtttg ggcattatgg cggacgatat ggcggctgcg gtgatgacct
     9901 tgattgtctt gaggattgca atgctgtttt aaacggggct gccttgtaaa aatgccgtct
     9961 gaaagccttt cagacggcat tgtttcggag gttaacgcgt taccggtttg tatttgatgc
    10021 gtttcggttt cgcgccttct tcgccgagtc ggcgtttctt gtcggcttcg tattcttgat
    10081 agttgccgtc gaagaacacc catttggagt cgccttcaca cgccaagata tgcgtggcta
    10141 tgcggtcgag aaaccagcgg tcgtgcgaaa tcaccatcac gctgccggca aattccaaca
    10201 atgcgtcttc caacgcgcgc agggtttcca cgtcgagatc gttggacggt tcgtccagca
    10261 gcaacacatt gccgccgccc aacaaggttt ttgccaagtg cagacggccg cgttcgccgc
    10321 cggaaagctg ccttgcgatt ttgctttggt cgctgccttt aaagttgaag cgtcccaaat
    10381 attggcgggc ggggatttca aactgtccga cctgcaaaat atcgcgacct tcggcaatgt
    10441 tgtcgaacac ggttttgtcg ttttgcaaac cttcgcggct ttggtcaatc aagctcattt
    10501 tcacggtttg cccgattttc acttcgcccg aatcgggctg ctctttgccc gcaatcattt
    10561 tgaacagcgt cgatttaccc gcgccgttcg ggccgatgat gccgacaatc gcgcccgccg
    10621 gcactttgaa gctcaaatcg tcaatcagca ctttatcgcc gaacgatttg gaaacattca
    10681 caaattcaat cacttcgtta cccaaacgct cggcaacagg gataaagatt tcctgagttt
    10741 cgttgcgttt ttggtattcg tagttgctca tttcttcaaa acgcgccaaa cgcgctttgg
    10801 gcttggcttg gcggcctttg gcattttggc gcacccattc caattcctgc ttcatcgcct
    10861 tcacgcgcgc ggcttcggat ttcgcctcgt tttccaagcg tttttctttc tgctccagcc
    10921 aagacgagta attgcctttc cacggaatgc cgtgtccgcg gtcgagttcc aaaatccatt
    10981 cggcggcgtt gtcgaggaag tagcggtcgt gcgttaccgc gaccactgtg ccggggaagc
    11041 gcacgaggaa ttgctccagc cattccaccg attccgcatc caagtggttg gtcggctcgt
    11101 ccaagagcag catatcgggc ttgctcaaca aaagtttgca caaagcgacg cggcgttttt
    11161 caccgcccga aagattgccg attttggcat cccaatcagg caggcgcagc gcgtcggcag
    11221 cgatttccaa ttcgtgttcc gcaccgccgc cagttgacga acccgccgcg ataatcgctt
    11281 ccaagcggcc ttgttcttcc gccagcgcgt caaaatccgc atcgggattg gcgtactcgg
    11341 catacacttc ttccaaacgt ttctgtgcgg cagccacttc gccgaaaccg ctttccactt
    11401 cctcgcgcac ggttttttcc ggatcaagct ccggctcttg cggcagatag ccgattttga
    11461 tgtcgcccat cggcacggct tcgccctcaa attctttatc cacgcccgcc ataatccgca
    11521 gcacggtgga cttgcccgtg ccgttcaagc ccaagaggcc gattttcgcg ccggggaaga
    11581 aagaaaggga aatatcttta atgatggttt tctgcggcgg cacaaccttg ctcacgcgca
    11641 gcatagaata gacgtattgt tgggacatag ttttctcgat tcggtcaaac aaatttcaga
    11701 cggccatttt aaccgataat ttgatttaag ccagtttatc cgcgaaccgg tattgccaaa
    11761 atcgggcagg attcataaaa tccgcctatc cctttgaaat tatacagaca aaaaaataat
    11821 aatgataggg gatcgccgcg ccggcaacca tttcggattt tccaaagcaa atacttttca
    11881 aatcaaaaaa aaggatttac tttatgtcgg aatatacgcc tcaaacagca aaacaaggtt
    11941 tgcccgcgcc ggcaaaaagc acgatttgga tgttgagctt cggctatctc ggcgttcaga
    12001 cggcctttac cctgcaaagc tcgcagatga gccgcatttt tcaaacgcta ggcgcagacc
    12061 cgcacaattt gggctggttt ttcatcctgc cgccgctggc ggggatgctg gttcagccga
    12121 tagtggctac tactcagacc gcacttggaa gccgcgcttg ggcggccgcc gcctgccgta
    12181 tctgctttac ggcacgctga ttgcggtcat cgtgatgatt ttgatgccga actcgggcag
    12241 cttcggtttc ggctatgcgt cgctggcggc cttgtcgttc ggcgcgctga tgattgcgct
    12301 gttggacgtg tcgtcgaata tggcgatgca gccgtttaag atgatggtcg gcgatatggt
    12361 caacgaggag cagaaaagct acgcctacgg gattcaaagt ttcttagcga atacggacgc
    12421 ggttgtggca gcgattctgc cgtttgtgtt cgcgtatatc ggtttggcga acactgccga
    12481 gaaaggcgtt gtgccacaaa ccgtggtcgt agcattctat gtgggtgcgg cgttactgat
    12541 tattaccagt gcgttcacaa tctccaaagt caaagaatac gacccggaaa cctacgcccg
    12601 ttaccacggc atcgatgtcg ccgcgaatca ggaaaaagcc aactggttcg aactcttaaa
    12661 aaccgcgcct aaagtgtttt ggacggttac tccggtacag tttttctgct ggttcgcctt
    12721 ccggtatatg tggacttact cggcaggcgc gattgcagaa aacgtctggc acactaccga
    12781 tgcgtcttcc gtaggccatc aggaggcggg caaccggtac ggcgttttgg cggcggtgta
    12841 gtcggttgcg gcggtgattt gttcgtttat tctggcaaaa gtaccgaata aataccataa
    12901 ggcgggttat ttcggctgtt tggctttggg cgcgctcggt ttcttctcta tcttcttcat
    12961 ctacaatcaa tacgcactca tcctgtctta tatcttaatc ggcatcgctt gggcgggcat
    13021 tatcacttat ccgctgacga ttgtggccaa cgctttgtcg ggcaaacaca tggatactta
    13081 tttgggcctg tttaacggct ctgtctgtat gccgcaaatc gtcgcttcgc tgttgagttt
    13141 cgtgcttttc ccgatgctgg gcggccatca ggcaaccatg ttcttggttg caggcgcagt
    13201 cttgctgctg ggagccttct cagtctgtct gattaaagag acccacggcg gggtttgagc
    13261 gatgagcgat acccccgcta cccgcgattt cggcctgatc gacgggcggg ccgtaaccgg
    13321 ctatgtgctg tccaaccggc gtggtacgtg cgtcttcgtg ctggacttgg gcgggattgt
    13381 gcaggaattt tccgttttgg cagacggcgt gcgcgaaaac cccgtggtgt cgttcgacga
    13441 tgcggcttcc tatgcggaca atccgtttca gattaacaag cagatagggc gcgtggccgg
    13501 acgcatccgc ggtgcggcgt tcgacatcaa cggtaggact taccgcgtgg aggccaacga
    13561 aggcaggaac gcgctgcacg gcggttcgca cgggctggcc gttacccgtt tcaacgcggt
    13621 ggcggcagac ggccgacggt tatcccaacg atttggatat ttcctaccgc ttggacgagg
    13681 atggccggct taccgttacc tatcgcgcca ccgcgctcgg cgacacggtg ttcgacccga
    13741 cgctgcacat ttactggcgg ctggacgcgg gcctgcacga tgcggttctg catattccgc
    13801 agggcggaca tattccggcc gatgccgaaa aactgcccgt cttaacggtt tcagacggcc
    13861 tcgaagtatt tgatttcagc cggcccaagc cgccggatgc cgccgttgcc gccctgcgcc
    13921 gcgaaacggg ccggttttga cgacgcttac cgcgtgccgt ccgatatagg ccgtcccgcc
    13981 gccgtgttgc aagccggacg ccgccgtcgt atcagcatat acagcgaccg caacggcttg
    14041 gtcatcttta ccgccgcccc gcaggatttc gcgcggcacg atgcgggcgt ttacgacgcg
    14101 ctggcgaccg aggcgcagac gctgcccgac agcctgcatt ggcccgaatt cggcaatatc
    14161 cgtctgaaca agggtgatac tagggaggcg acgattgctt acggcatcga atccctttct
    14221 taggagcttc ctaacaccgg tttcagacga cctttttata gtggattaac a
//
LOCUS       DAXDSD010000058        14218 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXDSD010000058 DAXDSD010000000
VERSION     DAXDSD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.058"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            782..1891
                     /gene="fic"
                     /locus_tag="ACFB2Q_001337"
     CDS             782..1891
                     /gene="fic"
                     /locus_tag="ACFB2Q_001337"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC0918358.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     GIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(2606..3418)
                     /gene="murI"
                     /locus_tag="ACFB2Q_001338"
     CDS             complement(2606..3418)
                     /gene="murI"
                     /locus_tag="ACFB2Q_001338"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC0918359.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            3607..4068
                     /gene="tsaE"
                     /locus_tag="ACFB2Q_001339"
     CDS             3607..4068
                     /gene="tsaE"
                     /locus_tag="ACFB2Q_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC0918360.1"
                     /translation="MSDFSPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            4065..5315
                     /locus_tag="ACFB2Q_001340"
     CDS             4065..5315
                     /locus_tag="ACFB2Q_001340"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC0918361.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADPAQNTTPRPGRGKNGRRPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            5397..6215
                     /locus_tag="ACFB2Q_001341"
     CDS             5397..6215
                     /locus_tag="ACFB2Q_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC0918362.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSPAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(6261..7403)
                     /locus_tag="ACFB2Q_001342"
     CDS             complement(6261..7403)
                     /locus_tag="ACFB2Q_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC0918363.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFAGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNGRRIVQTALDNAHNA
                     GADDIVSFSVAGAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLLPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(7452..7874)
                     /locus_tag="ACFB2Q_001343"
     CDS             complement(7452..7874)
                     /locus_tag="ACFB2Q_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918364.1"
                     /translation="MNKNRKLLLAALLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(7867..8676)
                     /locus_tag="ACFB2Q_001344"
     CDS             complement(7867..8676)
                     /locus_tag="ACFB2Q_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC0918365.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVAVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            complement(8758..9135)
                     /gene="acpS"
                     /locus_tag="ACFB2Q_001345"
     CDS             complement(8758..9135)
                     /gene="acpS"
                     /locus_tag="ACFB2Q_001345"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC0918366.1"
                     /translation="MIYGIGTDIVSLKRIVRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            complement(9185..9913)
                     /locus_tag="ACFB2Q_001346"
     CDS             complement(9185..9913)
                     /locus_tag="ACFB2Q_001346"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC0918367.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADNEQIQAARDVGAPVVELHTGAYADAH
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPVAQILAIRELNIGHSLISQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(9966..10709)
                     /gene="recO"
                     /locus_tag="ACFB2Q_001347"
     CDS             complement(9966..10709)
                     /gene="recO"
                     /locus_tag="ACFB2Q_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC0918368.1"
                     /translation="MSEHRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPASVSWYGSQELKTLHRAEWMGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMSELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTGALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(10743..11870)
                     /gene="pheA"
                     /locus_tag="ACFB2Q_001348"
     CDS             complement(10743..11870)
                     /gene="pheA"
                     /locus_tag="ACFB2Q_001348"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC0918369.1"
                     /translation="MLECTANYRSGEIMSQTIDELLIPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIVYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIRHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     RLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(11909..13138)
                     /locus_tag="ACFB2Q_001349"
                     /pseudo
     CDS             complement(11909..13138)
                     /locus_tag="ACFB2Q_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
     gene            complement(13254..14129)
                     /locus_tag="ACFB2Q_001350"
     CDS             complement(13254..14129)
                     /locus_tag="ACFB2Q_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC0918370.1"
                     /translation="MFYQILALIIWGSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
BASE COUNT         3285 a         4085 c         3825 g         3023 t
ORIGIN      
        1 tgccctctct ctaactctct cccacgggga gagaggacta tgatgcccac cggcggcttg
       61 ctattaggaa tatctgaatc gtcattccca cgaaagtggg aatctagaac gtcaaatctc
      121 aagaaaccgt tttatccgat aagtttctgt tccgacaggt ccggattccc gcctgcgcgg
      181 gaatgacgaa tccatccata cggaaacctg catcccgtca ttcccacgaa cctacattcc
      241 gtcattcccg cgaaagtggg aatctagttt tttgagtttc agtcatttcc gataaattgc
      301 cttagcattg aaggtccgga ttcccgcctg cgcgggaatg acgggatttg aggtttctgt
      361 ttttaatttt ctgttttcac gggaatgacg gtttaaaagt tacccgaaac ctcaaaaaaa
      421 agctgtgttt taatatagtg ttgatagacg tacttggcct ccatgtattc aatcgtggaa
      481 atctatatct tcgtcctcgc cgaaatagtc tatgcccgat atacaatttt gatacacaaa
      541 cttggaaata tgggtatcgt cgccggagcg atagaatgcg gacagttata tatatacggt
      601 ttttttaggg gagcgggcag atgaagggcg gattttgaat taaccccatg caaattgact
      661 tttgcgggcg gttttaccgc ccatatattt acaaaagcca aatttttaaa catatatcct
      721 tgatatatac acggcgtaaa catatactgg aaacatcttt aaattttccg aaattttaaa
      781 catgagcaac tggaaacccg atattcccta taacgattta ccacccctgc cgccaaaaca
      841 ggatattgaa agcaaaacca tcctgaaacg ttgtatagcc gcccgtgcat cccttgcccg
      901 tttaaagcag gcggcagaat tgataccgaa tcaagccatg ctgattaaca cccttcctgt
      961 tatggaagcc cgtgcaagtt cggaaattga aaacatcgta accaccacgg acaagctgtt
     1021 tcaatccctg caaatggata cggaacggca agaccctgcc acgaaagaag ccctgcaata
     1081 ccgcaccgcc ctgtttgcag gctatgaatc actggcgagc cgccctttat gcacacaaac
     1141 cgccatcatg gtctgcaacg ccatcaagca cccctacgaa acggccatcc gcaaaacagg
     1201 cggcacagcc ctaaaaggag gtaacagcgg aaatgttgtc tataccccgc ccgaaggaga
     1261 agaaaccata cgcggcaagc tggcaaattg ggagcggttt attcacgaaa gcggcgattt
     1321 agacccgctt gtcatcatgg cggcggcaca ttaccaattt gaagccatcc acccgtttac
     1381 ggacggcaac gggcggacgg gacgcatatt gaacagcctg ctattgattg aaaaagggct
     1441 tttggatttg cctattttgt atttgagccg ctacatcatc gaaaacaggg cggactatta
     1501 ccgcctgctt ttaggcgtaa ccgaacggca ggattgggaa agctggataa tctacatctt
     1561 agacggcgta gccgacaccg ccgattggac ggtatcgaaa atagatgcga tacgctgcct
     1621 gttcgagcag acacgtcaac acatacggac acacgcacaa ggaatctaca cgcacgaact
     1681 ggtaaatctt ctgtttgagc agccatatac acgcattgcc aacctagaag cggcagggat
     1741 agccaaacgg cagacggctt ctaagtacct gaaagagctt tcaggcatag gtgtgctgca
     1801 agaaatcgcc atcggcaggg acaaactatt cattcatccg cgcctaatgg aactattgcg
     1861 gggagagggc aacagcttta cttcattcta accccctctt catccctaca tgactaacac
     1921 gaaacaggga ttttgacacc cgaaccggta ccccttgtat tttccccgcg aaaagccggc
     1981 atccgcccgc gcatcatggg agcaacaaaa cccctgccta aaattttgac ttgtgcaaat
     2041 taggggtata ttgaaaaatg gctaaaataa aatgtttaat aaacaaaatg ttgaaactta
     2101 atttcgatag agcctctgca tatcgtattg aggcgttaat ggaatttgag aaagctattt
     2161 ttaaataaga aaaggtaact attaaatagc tacctttcta aaattaaata tcaacacatc
     2221 gttaaaacac agccaaaaaa aaccgaaacc gaacggtccg gattcccgcc tgcgcgggaa
     2281 tgacggattt caagattacg gtattgtcgg ggatgatggg aaacggtggg aattgtgtaa
     2341 aaaatgccgt ctgaagcctt tcaggcggca tttgcggcat tcggacattt aaaaatcccg
     2401 tcattcccgc gcaggaggga atccgaatac atccgcacag aaacctgcat cccgtcattc
     2461 ccgcgaaagc gggaatctag aacgtaaaat ctaaagaaac cgttttatcc gacaagtttc
     2521 cgcaccgaca ggtctagatt cccactttcg cgggaatgac gggattttag gtctctgtaa
     2581 tctcgggcag ctttccgtta tcgttttaac ccaaagacac catttcaatc tgctccatcg
     2641 tcctgcccag aaaacgctcg ccgatggttc tgaatttcaa aggaatatcg ctgacgtaaa
     2701 aacggtagtc gggattgttg ttgccggtat cgagcaatcc ttcctgtgca aggacgcgtg
     2761 cggtttcttc ggccgtcgta atcgcggaat cgaccagcgc gacattgtgc gcctccctgc
     2821 cgattaaggg cttgagcaag ggaaagtgcg tgcagcccaa caccagcgta tcgatgccgt
     2881 ctgcaagcag tggtttgagg tattcgcata cggtcaggcg ggtaacttcg tgttccaacc
     2941 aaccttcctc caccaaaggg acgagcagcg gcgcggcctg cgtgcggacg agcgtgtcgg
     3001 ggttgtccct gtggatggcg cgcgcataag cattgctgtt gactgtcgta ttggtggcga
     3061 tgatgccgat tttattgttg cgcgtcgttg ccagcgcggc tttggcgccg gcggaaatca
     3121 cgtccaaaac gggcatattg cccgtctttt gacggatttt ccgccccgca accgccgcaa
     3181 tcgtgttgca cgcgataacc atcgccttga catcgtgtcc caataaaaaa tcgacaatct
     3241 gcatcgagaa attttcgatg gtcgccttag atttcgtccc gtaaggcacg cgcgccgtgt
     3301 cgccgaaata aatgatgttc tccatcggca gccgttccat cagcgcgcgc acattggtca
     3361 aaccgccgat tcccgagtca aaaacgccga tgggccgctg cctgccgata ttttccatcc
     3421 tttttccaat ccgtcctaaa catacaatcc gcatattgta cacgcgccgc tttttcttga
     3481 cagccatgcc gtctgaaagc aggatgcgga tttggctgtt ataatgtacg gcaatgccgc
     3541 cgccgggcgc acggttttcc gaccgcctgc ccctccgatc cgccacataa aagttgtaaa
     3601 gcccctatgt ctgatttttc ccccgtttcc cgattccttg ccgacgaagc cgccacactc
     3661 gatttgggcg cggcgtggtc ttcccgttta aacgcaccgc tggtcattta cctcgaaggg
     3721 gatttgggtg cgggcaaaac cacgctgacg cgcggcatcc tgcgcggatt gggtcaccag
     3781 ggcgcagtca aaagcccgac ctacgccatc gtcgaatctt atccgctgga acgcttcgcc
     3841 ctgcaccatt tcgacctcta ccgcttctcg ttccccgaag aatgggaaga cgcggggctt
     3901 gacgagctgt ttgccgcaaa cagcgtctgc ctgatcgaat ggccgcaaca gggaggcgaa
     3961 tttaccccgc ctgccgacat caccgcaaca ttgacacacg gcggcggcgg cagaaaatgc
     4021 ctgctgaccg cccataccga acgaggacgc gaaagcctgc cgctatgacc aaactgacac
     4081 gaagacaaat catccgccgc accgccggca cactgttcgc cctaagcccc atcgcatccg
     4141 ccgttgccaa aacggtacgc gccccgcagt ttaccgccgc acggatatgg ccgtcgcaca
     4201 cctacacccg cctgacgctg gaaagcaccg ccgcgctcaa ataccagcac ttcgcgctcg
     4261 acaacccggg caggttggtc gtcgatatac aaaacgcaaa catcaatacc gtattgcacg
     4321 ggctttccca aaaagttatg gcggacgacc cctttatccg cagcatacgc gcgggtcaga
     4381 acacgccgac caccgtccgc ctcgtcatcg acctgaaaca gcccacccac gcacaagtct
     4441 tcgcgcttcc gcccgtcggc ggctttaagg accgcctcgt cgtcgacctc tatccgcacg
     4501 ggatggatgc cgacgatccg atgatggcgc tgctcaacgg cagcctgaac aaaaccctgc
     4561 gcggctctcc ggaagccgac cccgcccaaa acaccacgcc ccgacccggg cggggcaaaa
     4621 acgggcgcag acccgtcatc atgctcgatc cgggacacgg cggtgaagac cccggcgccg
     4681 tcagcccggg cggtttgcag gaaaaacacg tcgtcctctc catcgcccgc gaaaccaaaa
     4741 aacaattgga agccttgggt tacaacgtct ttatgacgcg caacgaagac gtgttcatcc
     4801 cattgggcgt gcgtgtcgcc aaagggcgcg cacggcgggc ggacgtattc gtctccatcc
     4861 acgccgacgc tttcaccagc ccctccgcgc gcggaacggg ggtttatatg ctgaacacca
     4921 aaggcgcgac cagctctgcc gccaaattct tggaacaaac ccaaaataat gccgacgcgg
     4981 tcggcggcgt accgaccagc ggcaaccgca acgtcgatac cgccctgctc gacatgacgc
     5041 aaaccgccac actgcgcgac agccgcaaac tcggcaaact ggtgcttgaa gaattgggca
     5101 ggctcaacca tctgcacaaa ggcagggtgg acgaagccaa tttcgccgtt ttgcgcgcac
     5161 ccgatatgcc gtctatcctg gtcgaaaccg ccttcctgtc caatcctgcc gaagagaagc
     5221 tgctgggcag cgaatccttc cgtcggcagt gcgcccaatc cattgcctcg ggtgtccaac
     5281 gctacatcaa tacatccgta ttgaagcggg gttgattgaa aaggcggtgt ttcaaaatat
     5341 agttgtcgga tgaaggcagg cataaaccta cttccgcccc aaaccgcgtt tttatgatga
     5401 aaggtatgga taaattgaga taccaacggg attttttaaa tatccgcccc atttttacag
     5461 ccggcgaaca agaatacctg accgaattgt ccgatcggct tcctttatct gttttaacgg
     5521 actccgtgcg taatattgaa gaaatcggta tcgattttgt gtacagtcct gccaaattgg
     5581 aaggaaacac ttataatcaa tacgatacgc aggcattgct taaactcggg caaaccgcag
     5641 gcgggaagtt atattcggat gccgtaatgt taatcaacct gagggaaagt taccggcacc
     5701 tgctgtccgg tttggacagc cccaagccgt ttgactggtt ggatttctta aaaaccaccc
     5761 acagcctgat ttcggaaaac ttactggaaa aaggctccgg cggcgtagtc cgccgcgaca
     5821 gcgttactat aagcgggaca gactacaccc ctttatccaa cccgcaaagt ttggatacgg
     5881 aattgaaatg gttgcttcaa gaagccccca aaatagaaaa tcctttcgac cgcgccgttt
     5941 atctccataa taatttggcg tatctccgat attttaagga ctgcaataaa cgtactgcca
     6001 gaaactgtat gaccttgtcg ctgatgcgct ccggtttttt tccttgcgta ttctcccccg
     6061 acagttatcc cgcctatgcc gaggctgtcg tagcctatta cgaaaccggc gattacggtt
     6121 tattcaagaa atattttatt tcggcatacg aaaataccgt aaataagtac ggtccccagc
     6181 ctgatgtgga tattttccga aatttctcca tttaaaaagc cgtctgaaca tttttcagac
     6241 ggcatttttg tacactttcc tcaacggttc gatccctgca ccatatcgat caggaacagg
     6301 cggcagtcga ggttgccgtt ataaagcggg attttccgct tgggcagcag gcgcatgaat
     6361 ttgggcattt ccctgtcgcc ggtaaacatg ccgacccgcc agccggcata gtgttttttc
     6421 aaccacgtac ccaactgcgg ataaagtgcc tgcaaagcgc ggacttcctc aaggcgcacg
     6481 ccgtagggcg gattggacac catgatgccg ttttcgccgt tcggacggac ggactgcgcg
     6541 ccggcaacgc tgaaggaaac gatgtcgtcc gctccggcat tgtgtgcgtt gtccaatgcc
     6601 gtctgaacga tgcggcggcc gttgtcgctg cctgcaatcg gggcgcggac ggggcgggtt
     6661 tgcgcttcgg cgcggcggcg caaatccgcc cacagcgttt tatcgaaatt ttgcagtttt
     6721 tcaaaaccga agcggcgcat catacccggc gcgcggccgg cggcaatcca agcggcttcg
     6781 atagcaatcg tgccgctgcc gcaaaacggg tcttgaaacg gctgcgtgcc gtcgtagcct
     6841 gccgagagca gcagcccggc ggcaaggttt tcgcgcagcg gggcttcgcc ggtatccagg
     6901 cggtagccgc gtttgaacag ggcttcgccc gaagtgtcga tgaagatttc gacattgcgt
     6961 tcgtccaaaa aggcgtggat gcggacatcg ggcgcggctt tgtccacgct cggacgtgcg
     7021 tcgtaaatgt cgcggaaagc gtcgcagacg gcatctttga cggtcaggcc ggcaaattgg
     7081 aggcttttga cgttggcgcg ttttgcctcg attttgactt tgaacgtctg ctgcaaggta
     7141 aaccagttga accagttgat gtttttggca agtttgtaga tgtcgcgctc ggttcggtat
     7201 gtgcctttgg tcaggcgcag caggatgcgg ccggcggtgc gcgagtgcag gttggcggcg
     7261 taggcctgtt ccaatccgcc ccggcaggaa acgccgccgt caaacgcttg gacatcggta
     7321 cagccgaggc ttccgagttc ttgagataaa acggtttcca agccgcgcgg gcaggtggcg
     7381 aaaagtgtat ataaagtatt catagcgtgt cctttccgga cggatgttcc ggcaatggcg
     7441 gattgaaagg tttattcggc ggtaaatgcc gtctgaaatg tccggctgcc gattgtaatg
     7501 tccgccgtaa aatcgcgcct gccttcgaca cagacgggca ggcggatgcg tgctgcctgc
     7561 caagtccccg acggttgccg ctcgaacata tagcggttga aacccatatc catatttttc
     7621 atactgaagc tgatgctgac ctgttccgtg ccggcgggcg cgtgttcgat ataaatatca
     7681 aacggttttt tggttgaaac ggcggcggcg cggacacggc ttccgtccgg cagcgtgcaa
     7741 ccctcggtca aatcgcattg cgccgccacg gcttgcggct gctgcgcctg ccaccattgc
     7801 aacaaaacga gcttgaaggc ggcaaaggca gtcagcagca gtgcggcaag cagtaatttg
     7861 cggtttttat tcatgattct tccctattgt ttcatccctt accaccgcgt gcgcccccaa
     7921 tccgagccat agttttccat aacgtgcaac cgtctgtccg tttgctgcaa gtacgagcgg
     7981 cacggatacg ccctgccgca gggcttctgc cgcctcttgt gcctgaacat catcgcacac
     8041 gcgccacacg gccacatccg cgcccttttc ctgcgcctgc cgccattgtt cgcgggtctg
     8101 caccatcatc cacacgctgc cggcgtgttc ggacctgtcc tgccattgtg ccgcctccat
     8161 caaaacgttc gcaccgcttc cccctgccga acccaaaggc aggacgcggt acgcgcccat
     8221 actgttttcg ccgtacaaac ctgttttcag gctgccgtac aaacggcgcg ggacggacag
     8281 cgaacgcaaa agcgcgccgt tggcaggcag catgggggca acggtaaaat cacccgcctt
     8341 ctgccaagac cattcctgcc cttcgcggga ttgcggtccg ccctcccatt gatcggggtt
     8401 tacccataag aatttcaggc agacgcgggc gtgttcgtag gaatggattt tggtcagcca
     8461 aggcgtggcg gcgaggatgc ggatgccgag ttcttctcca aactcgcgtt gcagggcttg
     8521 gaagtcggtt tcgcccgcct cgaccttgcc gccggcaaat tcccaatatc cggcataggg
     8581 cttgccttcg gggcgcgagc cgagcaggta attgccgtct gaatcgagca ggatgccggc
     8641 aacgacgcgg ataagggggc gggtatcctg gatcatggcg ggtatgcggt tggtcggtcg
     8701 gaatgcaaat gagggaaggg caatgccgtc aacgggtttg ccgaatttca gacggcatta
     8761 tttttcggca acggcaaacg ccaacacggt atcgccttcg tccgccatac tcaggctgac
     8821 gcggctgatg ccttgttctt ccagccattc ggacagggcg gggccgtaga aaaattcggg
     8881 cttgcccaat gcgtcgtgcc cgatgccgat gttgcggaag gaaaccgcgc ctcgtatgcc
     8941 cgtgccgacg gctttggcga aggcttcttt ggcggcaaag cgtttggcaa ggtagttgac
     9001 gggtttgccc gcctgcggaa attcaagcag ctcttcggga gtgagtatgc gccctgcaaa
     9061 cgcctgtccg aattttttgt ttaagcggac gatgcgcttg agggaaacga tgtctgtacc
     9121 gatgccgtaa atcatatttg cgctcctttt tcgtgaaatg ccgtctgaaa cggtttgcct
     9181 gcccttacgg cagcagcctt gccctgaaca tcgcctcctt catctggcgc acggcctcgg
     9241 gcaatccgag gaaaagtgcc tgcgaaatca gcgaatgccc gatgttcagc tcgcggatgg
     9301 cgaggatttg ggcgacgggg gtaacgttgt gtatggtcag tccgtgtccg gcgttgacga
     9361 ccaagcccaa atcgccggcg aaatgcgcgc cgttttgcag acgctcgaat tgcttgagtt
     9421 gttcggcgtg gctgtgtgca tcggcatatg cgccggtgtg cagctcgaca acgggcgcgc
     9481 cgacatcacg ggctgcttgg atttgctcgt tgtcggcatc gatgaagagc gagactcgga
     9541 tgcctgcgtc ggtcaggatt ttggtgaacc cggcaatttt ttcttgttgc gccaatacat
     9601 ccaagccgcc ttcggtcgtg atttcctgcc gtttttcagg cacgatgcac acgtcttccg
     9661 gcatcacttt aagcgcgttt tcgagcattt cttccgtcaa cgccatttca aggttcaggc
     9721 gcgtgcggat ggcgtttttg acggcaaata catccgcgtc tttgatgtgg cggcggtctt
     9781 cgcgcaggtg catggtaatc aggtcggcac cgtgcgtttc ggcaaccagt gccgcctcca
     9841 cggggctggg ataagtcgtg ccgcgcgcgt tgcggacggt ggcgatgtgg tcgatgttga
     9901 cacccagaag cataatgttt ccttttctct tcgttttcct gtttgacggc atggtgcggg
     9961 cgcagctacg cggtattgcg gtcgaactgc cttatctgtt ccaacacctg ccgcgatttc
    10021 aagccttcgg gaagcagcgt gccgattagc agccgtgtga ttttcaatgc ctgttgcagg
    10081 ctttcggcgg tgcggaaact gccttcgcgc aaatcgatca ggctctgccc ttcgacaatt
    10141 gcgccggctt cgtgcgaaag cgcgcctgtg tcccggcata cgggcatgac cgcctcttcg
    10201 ggcatcagcc ggtaggtttt gtccgccaaa atatcgccgc ccgtgccgtc ggcgtgcaaa
    10261 tcgggggcaa cgcccaaggc attgagcagc ttccattcga agcggcgcaa atcggcaata
    10321 tggttggctt cacggcacac tgcctccatc acttttgcca acgcgtcgta cagttcggac
    10381 atcgggtctt cgcgcgcggt cagtttcaac accagctcgt tcacatacag accgctgaac
    10441 aacgccctgc cctgcggctg ccgccatccg cccatccatt cggcgcggtg cagggttttg
    10501 agttcctgac tgccgtacca cgacacgctg gcagggacga acggaaccaa tacgccgcgc
    10561 agctcgctct gccgtttgcg cgcgctgcgc gccagcaaag ccacacgccc gtaacggcgg
    10621 ctgaatgctt caacccgcag gctgctttcg cgccaaggcg aagatgccag cataaaaacg
    10681 ggttcgtggt tgactcggtg ttcggacata atgcctgaaa tgccgtctga acgctgccgc
    10741 gactacaaaa cggcagtcgg atacgaaccg atgactttga caaacgaagc gcgttcgccc
    10801 aagcgttcca atgccgtctg aatctgcgcg tcccggcggt gtccttcgat gtcgatgaag
    10861 aacaggtatt cccacaaaac ggatttgctc ggacggctct caaacttggt catggaaata
    10921 cccgattcgg tcagcggttg cagcagcgag gcaaccgcgc cggcgcggtt gggcgcggaa
    10981 acggccagcg aggttttgtc gctgccgctt gcaccggttt cgtgatgtcc catgaccaga
    11041 aagcgcgtgg tgttgttcgg ttcgtcttcg atgcactcgg caaccatatc gagtccgtaa
    11101 atttccgccg ccgtgcgtcc ggcgatggcg gcaaccgtac cgtcgtccga ttcggcaacc
    11161 agccttgcgg cttcggcatt gctggacacg gcaatccgtt cggcgttggg cagacgcctg
    11221 cccaaccagt cgttgcactg cgccaacgcc tgcgcgtgcg aaaatacttt ggcgataccc
    11281 tcggtgctgc cgttgttttt gcgcaacagg ttgtggcgga tgcgcaaaac gacttcgccg
    11341 cacgcctgca acgcggtaac ggcaagcaaa tccaatgtgc gcccgaccga gccttcggtc
    11401 gagttttcca cgggcgcgac cagataatcc gcctgacggg tttcaacctg tttgaagcag
    11461 tcgtctatgg tcggacacgc cattgtatgc gcggcatgac cgaaatgctt aatcgccgcc
    11521 tgctgggtaa acgtgccctg cggccccaga tagacaatgg tcagcggacg ttcgacggcg
    11581 aggcactcgc tcatcacttc ccgaaacagg cgtgctaccg attcgtcggg cagcgggcct
    11641 ttgttcaaat cctgaatgcg gcgcaacacg gcaacctcgc gttcggggcg gtacaccgcg
    11701 cccgtgcctt tcagctcgcc gatggcgtgg gcatgttgcg cgcgttcgtt gagcaggcgc
    11761 aggatttcgg catcgatggt gtcgatggcg ttgcggtgcg ggatgaggag ttcgtcgata
    11821 gtttgggaca taatttcgcc tgaacggtaa tttgccgtgc attctaacat caaatgccgt
    11881 ctgaagcggt ttcagacggc attttccgtt aaaggtattc gctttgcccg ttttctttcc
    11941 acgcacgatg cgagcagagc cacaaaagcg caatgccgca agaggcggac gcggtcatgg
    12001 ttgccgccat cacggttgcc gaaccgtcgt gcaggacggt tgccgccatg cccacgcccg
    12061 cgccgattaa ggaccggaat acacccgaca cggcgttcgc gctgccgccc tcttctttga
    12121 aataagacat aaagcatgcc tgcgtgtccg caccgaccag gccctgcgta ccgacggaaa
    12181 acatcacgca cgcaaccggc agccaaaacg ggggcaaccc gaaaaacagc acggcggcga
    12241 gttgggacgg gttggcggca aattggacga caatcccccg cagcaggatg ctttgcggat
    12301 gcgcgccggt tttaagccgc cacgcggtaa cgcggctgaa aaacatcatc gtgatgatgt
    12361 tgagtgcaaa cacccatgcg taccggtgcg gcgtaacgtg gtagagctgc cggtacacga
    12421 aggaagattc ggtcagaaag gcgaacatcg aaccgaagct gaatgcctga aaaaacagat
    12481 aacccatcgc ggcacgggtt ttcaatacgc gcttgaaccg ccccgccacc agcccgaaca
    12541 catccctgcc gattttgccg ccgacggcgg gattgggcag gaaatactgt accaaaccgg
    12601 gcagcaccgg cgaatacgcc gccaagaaaa cgaaaatcgc ccgccatccg cccaatccct
    12661 gcaacaatgc gccgaccatg ggtgcggcca gcggcacaac catcagaatg atgccgataa
    12721 gggcaaacat ctgcgcggct ttgcgtccgg aataataatc gcgcaccatc gcaccgacga
    12781 tgactacagc catgcctgcg ccgaacgcct gtaccgcacg caggttaagg agctgttcgg
    12841 tactcgaagc aaatacgatg gcggcaacgg caaggcaata tacaatcaaa ccggtcaggg
    12901 cgacgggttt gcgccctttg atgtcggaca ccgcgccgcc ggccacttgc ccgaacgccg
    12961 tgccgaacat aaacaaactc agactctatt cgatacggtg gatatccgcg ttcagcggct
    13021 gcgccatttc gggaatcgcg ggcaggtagg catcggtgga aaacggcatc agcgcgacca
    13081 gtaccgccat caaaaccgcc atcagttttc cgctcatcgg aggaaggggc ggagacatgg
    13141 tttttccgtt caaatcaaat aaagaaaccg tcgcaaacga cggtcggtac gggcgcaagt
    13201 ctaacatatc ggacggcggg gcaaaaccga aggatatgcc gcccgaaacc gcttcagacg
    13261 gcattgccgt tttgcgcgtc cctgcgcgac agccggccgg cggcgaaagt ggcggcgatg
    13321 acgacaaaca cgcccaaggc ggacacgggc gataaatgtt cgcccaaaat caaaaccgcc
    13381 aacagcacgc cgacgacggg ttcgagcgaa atcaacagtc ccgacgcgtt ggcaggaaca
    13441 cggctcatcc ccttgttcca cagccaatag gcgtaccagc cgcaccccaa acccaaatac
    13501 aacagcgaca ataccatccc gacgctccag tccacggtat aactttgcgc caaagcaagc
    13561 gaaaacggca ggcacatcaa cgatgcggcg gcaatggaaa cagatgtgaa tgccggtgcg
    13621 ccgatgcggg caatcagcct ttgcgtcgga cgcatagcgg cacaaaagcc cgcgcccgcc
    13681 aacaacacca gcaggcagcc gaaccagccg acttcgccgc cctcttccgc accgcccgcc
    13741 atcagcagcg cgacaccggc aaatgccgcc gcgccgcata tccagtggta ggcacgcgct
    13801 ttgtcgttga agaaaaagtg tccgacaaac accatcagca gcggctcgag tccgacaatg
    13861 accgatgcgc tggcggcgga agtgtatttc aacccgacaa actgaagcag cagggtcagc
    13921 acatagttga cgaacgacac aatcagcaac ggcttccatt cctcacgcgg aatcttgccg
    13981 acatgacggc ggcaggcggg cagtgcaggc agcgcggcaa tcagcaggcg cacgccgacc
    14041 atcaatgcgg gatcgatgcc gccatagaca tatttggcgg caataaacga gctgccccag
    14101 ataatcaggg caaggatttg gtaaaacata gtgagtggta aagtcagatg gtcatacaaa
    14161 agtatagcgg attaacaaaa atcaggacaa gaaggaatgc ccgaaccgtc attcccgc
//
LOCUS       DAXDSD010000059        14124 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXDSD010000059 DAXDSD010000000
VERSION     DAXDSD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14124
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.059"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            290..553
                     /locus_tag="ACFB2Q_001351"
     CDS             290..553
                     /locus_tag="ACFB2Q_001351"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918371.1"
                     /translation="MSSAQQANYPQNLFPTKNNPQVCTKGNPPYRHPFKTAIQMILKI
                     KKSYPQKIPHINIIIVLLFKPYILFITLQGNGTAKRKGIFSKN"
     gene            633..2081
                     /gene="gnd"
                     /locus_tag="ACFB2Q_001352"
     CDS             633..2081
                     /gene="gnd"
                     /locus_tag="ACFB2Q_001352"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC0918372.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWGLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2144..3415
                     /gene="waaA"
                     /locus_tag="ACFB2Q_001353"
     CDS             2144..3415
                     /gene="waaA"
                     /locus_tag="ACFB2Q_001353"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC0918373.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3456..3905
                     /locus_tag="ACFB2Q_001354"
     CDS             3456..3905
                     /locus_tag="ACFB2Q_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC0918374.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPISGEPFATSFGTMLTLKILLAFSV
                     LAHFAVAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            3938..4912
                     /locus_tag="ACFB2Q_001355"
     CDS             3938..4912
                     /locus_tag="ACFB2Q_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC0918375.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            4981..5056
                     /locus_tag="ACFB2Q_001356"
     tRNA            4981..5056
                     /locus_tag="ACFB2Q_001356"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5014..5016,aa:Lys,seq:ttt)
     gene            5277..6530
                     /gene="murA"
                     /locus_tag="ACFB2Q_001357"
     CDS             5277..6530
                     /gene="murA"
                     /locus_tag="ACFB2Q_001357"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC0918376.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            6611..7804
                     /locus_tag="ACFB2Q_001358"
     CDS             6611..7804
                     /locus_tag="ACFB2Q_001358"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC0918377.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDTLGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(7856..8104)
                     /locus_tag="ACFB2Q_001359"
     CDS             complement(7856..8104)
                     /locus_tag="ACFB2Q_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC0918378.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8197..9114)
                     /gene="ftsX"
                     /locus_tag="ACFB2Q_001360"
     CDS             complement(8197..9114)
                     /gene="ftsX"
                     /locus_tag="ACFB2Q_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC0918379.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            complement(9111..9761)
                     /locus_tag="ACFB2Q_001361"
     CDS             complement(9111..9761)
                     /locus_tag="ACFB2Q_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC0918380.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9955..10437)
                     /locus_tag="ACFB2Q_001362"
     CDS             complement(9955..10437)
                     /locus_tag="ACFB2Q_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC0918381.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10561..10914
                     /gene="arsC"
                     /locus_tag="ACFB2Q_001363"
     CDS             10561..10914
                     /gene="arsC"
                     /locus_tag="ACFB2Q_001363"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC0918382.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEAVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10911..11567
                     /locus_tag="ACFB2Q_001364"
     CDS             10911..11567
                     /locus_tag="ACFB2Q_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC0918383.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11683..13077
                     /gene="gltX"
                     /locus_tag="ACFB2Q_001365"
     CDS             11683..13077
                     /gene="gltX"
                     /locus_tag="ACFB2Q_001365"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC0918384.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            13098..13493
                     /locus_tag="ACFB2Q_001366"
     CDS             13098..13493
                     /locus_tag="ACFB2Q_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918385.1"
                     /translation="MPSDVGMRLQTAFRRKLNMKKILYFLMFVFSTSVWAGGAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEEMRQIGVRLDHAWNAVYGAGERKRRGN"
     gene            13497..13988
                     /locus_tag="ACFB2Q_001367"
     CDS             13497..13988
                     /locus_tag="ACFB2Q_001367"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC0918386.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
BASE COUNT         3212 a         3503 c         4148 g         3261 t
ORIGIN      
        1 gtactgcctg cggcccgccg ccttgtcctg attttcgtta atccgctatg cttatacaca
       61 accagccaaa agccatctta ttgaaatatc ataagattta ctcaaaaatc ttattttctc
      121 caccacttat gcacaaactc ggatacacac tctgccaata tgcagaaaaa aaagacattc
      181 gcaccgacac cttttcatcc ccacaagttc caaacacatt tccggcttaa atatccacag
      241 cattcccgtt cttaaaggca ccggcagcca ttcggtaaag aaaagcggga tgtcgtccgc
      301 acaacaagca aactatccac aaaacctatt ccccacgaaa aacaatccac aggtatgcac
      361 aaaagggaat cctccttacc gacacccttt taaaacggca attcagatga ttttaaaaat
      421 aaaaaaaagt tacccacaga aaattcccca tatcaatata atcatcgttt tattatttaa
      481 accttatatt ttatttataa cccttcaggg aaacggcacg gcaaagcgta aaggcatctt
      541 ttcaaaaaat taaaaagtta aggattgtta aacaagtgta aatcaggtag agttgcagcc
      601 gataaattaa atctataacg gagcttcctg caatgaaagg cgatattggc gtaatcggtt
      661 tggcggttat gggtcaaaac ctgattttga atatgaacga ttgcggattt aaggttgtcg
      721 cctacaaccg gacaatcggt aaagtggacg aatttttaaa cggtgcggca aaagagacgg
      781 gcattatcgg cgcgtattcc ctgcaagact tggtcgataa gctggcaaaa ccgagaaaaa
      841 tcatgatgat ggttcgcgca ggttcggtgg ttgacgactt tgtcgaacaa ctgcttccgc
      901 tgttggaaga aggcgacatc attatcgacg gcggcaatgc caattacccc gacacgacac
      961 ggcggacaca ttaccttgcc ggaaaaggga ttttgtttgt cggcgcgggc gtatccggcg
     1021 gcgaagaagg cgcgcggcgc gggccgtcca tcatgccggg cggggataag cgcgcttggg
     1081 aagcggtcaa gccgattttc caggcgattg ccgccaaaac gccgcagggc gaaccgtgtt
     1141 gcgactgggt cggcaaggat ggcgcgggac attttgtcaa aatggtgcac aacggcatcg
     1201 aatacggcga tatgcagctg atttgcgaag cgtaccagtt tatgaaagac ggtttggggc
     1261 tgtcctacga cgaaatgcac cgcgtgtttg ccgagtggaa caaaaccgag ctggattctt
     1321 atctgattga aatcacagcg gcaattttgg gctataagga cgaaggcggc gaaccgctgg
     1381 ccgagaaaat cctcgatacg gcagggcaaa aaggcacggg caaatggacg ggcatcaatg
     1441 ccttggattt gggcattccg ctgacgctga tttccgaagc cgtattcgcc cgctgtgttt
     1501 cgtcgttcaa agaacagcgc gtgcagaccg gcaagctgtt cgcccgaacc gccacgcccg
     1561 tcgaaggcgg caaacaagaa tgggtcgagg cgttgagaca ggctttgctt gcctccaaaa
     1621 tcatttccta cgcacaaggc tttatgttga tccgcgaagc gggcgaaagt tacggctggg
     1681 gtttggacta cggcaacact gcgctgctgt ggcgcgaagg ctgcatcatc cgcagcgcgt
     1741 ttttgggcaa tatccgcgac gcgtatgagg ccaatcccga tttggtgttc ttgggtgcgg
     1801 atccgtattt caaaaatatt ttggaaaact gcctgccggc atggcgcaag gtggttgcca
     1861 aggcggtcga atgcggcatc cctatgccct gcatggcttc ggcgattacg ttcttggacg
     1921 gctatacgac cgaacgcctg cctgccaacc tcttgcaggc acagcgcgac tacttcggcg
     1981 cgcacactta cgagcgcacc gacaaaccgc gcggcgaatt tttccatacc aactggacgg
     2041 gcaagggcgg agataccgct tcgacaacct acgatatttg attccgttgc cgaaatgccg
     2101 tccgaaccgt tcggacggca tttgttttgc aggaaataga aagatgttcc aatggcttta
     2161 tgatgtattg tggctgcttg cgccgatatg gatacggcgt tatttggaca aacgctccgg
     2221 aagtgccccg gcataccggg cgcataggga cgagcgtttc ggcaagccgc atccgaatcc
     2281 cgttaccggc gcggtttgga tacacgccgt ttcggtcgga gaaacgcgtg ccgcccagcc
     2341 cttgatacgc gagttgcggc ggcgttttcc cgatgcgccg ctgctgatga cgcagatgac
     2401 cccgacggga cgggaaaccg cgcaagttct gtttcccgat gcgcaatgcc gctatcttcc
     2461 gtatgacaaa aaaacgtggg tacggcagtt tttgcgcgaa caccgcccga tgttcggcat
     2521 tttgatggaa accgaaatct ggcccaatct gatgaaggaa tgccggcgag cgggtgtgcc
     2581 gctgtttttg gcgaatgcgc ggctgtcgga aaaatcgctg aacggttatc tgaaagtccg
     2641 ccgcctgatc cgtcccgcag ccgcttcgct gacgggctgt ctggcgcaga cggaggcgga
     2701 tgcggcgcgg ttggcgaaat tgggcgcggc atccgtgcag gtgtgcggca ataccaaata
     2761 cgacctgatg ccgtctgaag acatgaaaac gctggcgggg cagtttgaaa aacgcatcgg
     2821 aggccggccg gttgccgtgt gcggcagcac gcgcgtttat cggggcgaag acgaggcgga
     2881 aaaactgctg gcggcgtggc aacaatatcg cggcgatgcc ctgctggccg tcgtgccgcg
     2941 ccatcccgag cattttcaga cgacatttga aacggcgaaa cgcttcgggt ttaaggttca
     3001 gcggcgcagc gacggtttgc cggtcgaacc cgatacgcag gtgtggatag gcgacagtat
     3061 gggcgagctg tatgcgtatt acctgtgcgc cgatgtcgct tttgtcggcg gcagtctggt
     3121 cggttcgggc tgtcagaaca tcatcgaacc gctttcctgc ggcgttacga cgatattcgg
     3181 cttttcgacc tacaattttt ccgaagcctg ccggcacgcc ttggcatcgg gtgcggcggt
     3241 tcaagtcgaa tcggcggacg cgtggcgtga agccgttgaa aaaaacttat ctggcgaggg
     3301 gggcggaatg cagatgcagg cgcgcgtgga cggctttatc gcacaacatc gcggagcggg
     3361 cgcgagaatc gccgaggcgg tgcgggaagc ggtatgcgga catcgggggc gatagtgata
     3421 caatccgtat ccgagttttc cggttggagt accgtatgag tatttatgcc gtcgcgcaca
     3481 tcatccacct gtattgcgcc accgcctttg tcggcggcgt gttttttgaa gtgctggttt
     3541 tgtccgtcct gcatacggga cgggtgtcgc gcgaggcgcg gcgcgaagtg gaaaaggcaa
     3601 tgtcttaccg cgccgtcagg gtgatgccgt ttgcggtcgg actgctgttc gccagcggca
     3661 tcgtgatggc ggcaaaccgc taccttccta tatcgggcga accgtttgcc acttccttcg
     3721 gtacgatgct gacgctgaaa atcctgttgg cgttcagcgt gttggcgcac ttcgccgtcg
     3781 ccgtcgtcaa aatggcgcgt tccacgctga cggtcggctg gtcgaaatac atacacgccg
     3841 tcgtctttac ccatatgctg ctgattgtct ttttggcaaa agcgatgttt tatatcagct
     3901 ggtaacacaa ctacatttca aatcaggaga ttgaaccatg acagaatatc ccggcatcgg
     3961 gtcgccgttg ttttacggcg ttttttttgc ggcagtgctg gtcatgattg ccttggatat
     4021 gttttcgctg aagaaaaacg gcagccataa agtcggcatc aaagaaacct tggcatggag
     4081 cggcttgtgg gttgccgtat cctgcctgtt cgcaggctgg ctgtatttcg aactcgccgg
     4141 caatcccggc tacggcgcgg cagccgcaaa agaaaaagta ttggaattct ttaccggcta
     4201 cattttggaa aaatcgttgg cagtcgacaa tattttcgtg ttcctgatga tattcggcta
     4261 cttcaaagtc gcaccgcagt ttcaacaccg cgtgctgctg tacggcgtat tgggcgcatt
     4321 ggtattgcgc accgtcatga ttttcgtcgg cgcggcactg gttcggcagt tcgagtggat
     4381 tctgtatctg ttcggcgcgt tcctgctcta taccggcata cacatgatga agcccgaagg
     4441 agatgaggaa gaggatttgg caaacagcag gctgctgaat gccgtcaaga aagtcgttcc
     4501 ggtcggcacg gcgtttcacg gcgagaaatt ttttaccgtc gagaatggca aaaaaatcgc
     4561 cacgccgctg tttttggtgc tgattatgat tgaattgagc gatgtcgttt ttgccgtgga
     4621 cagtatcccc gccgtctttg ccgttaccac cgatccgttt atcgtgctga cttccaatat
     4681 tttcgctatt ttgggtttgc gggcgatgta tttcctgctg gcggatgtgg cggaacgctt
     4741 tatcttcctg aaatacggct tggcattcgt gttgggtttt atcggtgtga aaatgctggt
     4801 gatgcattgg gtacacatcc cgatttccgt ttcgctgtcg gtcgtgttcg gcgcgttggg
     4861 tgcatcgata ctgacctcgt taatttatac gaaaaaacag cctgataaat aaaacgccgt
     4921 ctgaacgccg acaaaatggt tgacagcatc aggcttgatg tataaacttt caaatttttc
     4981 gggtcgttag ctcagtcggt agagcagcgg acttttaatc cgttggtcga gcgttcgaat
     5041 cgctcacgac ccaccaggta aagcaaaaaa gccgagtttt caaaactcgg cttttttttg
     5101 ttttccgaca atccttgttc ggaaagcgcg gcatttcgtc aaattctttg gtgcggcttc
     5161 cgtatttttc ccgaaaatcc cttataattc cgcctctgca accttcatcg ggtgatgctt
     5221 tatgcattgt cccgagtatt gaaatattag caagtaccca ccaggaggga acaaacgtgg
     5281 acaaactgaa aatctccgca aacggcccgc tcaacgggga aataacggtc tcgggcgcga
     5341 aaaacgcggc attgccgctg atgtgcgcgg gtttgctgac gtcgggtacg ttgcgcctga
     5401 aaaacgtccc tatgctggca gatgtcgcaa ccacgcaaaa gctgcttcag ggcatgggcg
     5461 cgcgcgttct gaccgacaat atcagcgaat ttgaaatcaa cggcggtacg gtcaacaata
     5521 cctgcgcccc ttacgaattg gttcggacga tgcgcgcttc gattttggtg cttggcccga
     5581 cgctggcgcg tttcggcgag gcgcaagtca gcctgccggg cggctgcgcc atcggttcgc
     5641 gccccgtcaa tcagcatttg aaaggcttgg aagcgatggg ggcggaaatc gccatcgaac
     5701 acggttacgt caaagccaaa ggcaaactca aaggtgcgcg cgtggcgatg gatgtcgtta
     5761 ccgtcggcgg tacggaaaac ctgctgatgg cggcgacgct ggcggaaggt acgacggttt
     5821 tggaaaactg cgccatcgag cctgaagtgg tcgatttggc ggaatgcctg gtcaaaatgg
     5881 gcgcgaaaat cagcggcatc ggaacatcga ccatgattgt ggaaggtgcg ggcgagctgt
     5941 acggctgcga acacagcgtc gtccccgacc ggattgaagc ggggacgttc ctgtgtgcgg
     6001 tggcgataac cggcggcagg gtggttttgc ggaatgccgc cccgaaaacg atggaagtgg
     6061 tgttggacaa actggtcgag gcaggtgcgg tgattgaagc gggcgacgat tggatcgcca
     6121 tcgatatgcg gcagcgtccg aaggcggtgg acatccgcac ggtcgtccat cccggcttcc
     6181 ccacggatat gcaggcgcag tttatggcat tgaatgctgt ggcggaagga agctgccgcg
     6241 tggtggaaac gatttttgaa aaccgcttta tgcacgttcc ggagttgaac cggatggggg
     6301 cgaacattac aaccgagggc aatacggcat ttgtgcaggg tgtggaacgg ctttccggcg
     6361 cggtcgtcaa ggcgacggat ttgcgcgcgt ccgccagcct cgttatcgcc ggtttggcgg
     6421 cgcgcggcga aaccgtggtc gaacggattt accacttgga tcgcggttat gaaaatattg
     6481 aaaaaaaact cggcagcgtc ggtgcgaaca tcgagcgcgt atccggctga tcgggttgcg
     6541 atgccgtctg aaggcttcag gcggcatcaa ttgtttctgt atcgcaaaca ctgattgaaa
     6601 aggactgatt atggcatttt taaaactgac cgaacaaaac gtgcggggca aaaccgtcct
     6661 catccgcgcc gatatgaacg tgccgttcaa aggcggcaaa atcagcgacg acacccgtat
     6721 ccgcgcctcg ctcgcgtccg tcaaatactg cttggacaac ggcgcgtccg ttattgtgat
     6781 gacccacttg ggccgcccga ccgaaggcga gttccacccc gaagacgacg ttgcgcccgt
     6841 tgccgcgcac ttgggcggcc tgttgggcaa agacgtgaaa gtattgaacg actggcgcga
     6901 aaacaaaccg gctttgaacg cgggcgatgt cgtgatgctg caaaacgtgc gtatcaacaa
     6961 aggcgagaag aaaaacgact tggaactggg taaagcctac gccgccttgt gcgacgtgtt
     7021 cgtcaatgat gcgttcggca ccgcacaccg cgcccaagct tcgaccgaag ccgttgccca
     7081 agccgcgccc gtcgcctgcg ccggcgtatt gatggcgggc gaactcgaca ctttgggcaa
     7141 agccctgaaa cagcccgcgc gcccgatggt ggcgattgtt gccggcagca aagtgtccac
     7201 caagctgacc atcctcgaat cgttggcgga caaagtcgat cagcttatcg tcggcggcgg
     7261 catcgccaac acattcctgt tggcggaagg caaagccatc ggtaaatcgc tggcggaaca
     7321 tgatttggtg gaagaatcca aaaaaattat ggcgaaaatg gcggccaaag gcggttctgt
     7381 gccgctgccg accgatgtcg tcgttgccaa agcctttgcc gccgatgccg aagcggtggt
     7441 aaaagacatc gccgacgttg ccgaagacga gatgattttg gacatcggcc cgaaatccgc
     7501 cgccgcgctt gccgagttgc ttaaagccgc cggtacggtc gtttggaacg ggccggtcgg
     7561 cgtgttcgag ttcgatcaat tcgcaggcgg cacgaaagcc cttgccgaag ccatcgccca
     7621 aagcaaagcg ttctcgattg cgggcggcgg cgacacgctg gcggcgattg ccaaattcgg
     7681 cgttaccgac caaatcggct acatctccac cggaggcggc gcgttccttg agttcttgga
     7741 aggcaaagag ctgcctgccg tagccgcttt ggaaaaaacg cggcgagtaa acggtttgat
     7801 ataaaaaaat gccgtctgaa acgaaatggt ttcagacggc atttttgtgt ggcggttatt
     7861 gtgctttggc agcccattcc gccggagtgg cggcaaccga aatggaaagt gcgtgcagtt
     7921 cgttgctttc cagttgggct ttgagtccgt ctttaatcag gcggtggcgc gcgaggcgtg
     7981 ccttgccttc aaattctgat gaaacgatga cggcgaaaaa atggtgtccg tcgccttcta
     8041 cttcgatgtg ttcgcatttt gccacgcctg caatcagggc cttgacttgt tcgggtgtca
     8101 gcatagtggg gagtccttat tggggaaatg cgccattata ccggcaaatc gaaattccgt
     8161 ctgaaaacgg cttcagacgg aatttttgac ggtgttttat tttttggctt tgaagcagag
     8221 caggtgttgt gtggtggcaa gccacgcgcc gaatacgccc aacgcgatga cgaagccgaa
     8281 caccagcccg agttcgccga cgtagaagaa acgccagccg atgttcaagc cgtagggttt
     8341 gaagatggca tcgaccaagg ggcgcacggc agagagcagc caaccgcaaa gccctaagct
     8401 gacggcggcg gagaagatac tctgccacat agcctgataa aggaaggggc ggcggataaa
     8461 cgacgcgggc gcacccagga gtttggtgat ttcgatttct tctttgcggc tgaggatttg
     8521 cagtcggatg gtgttgtgtg cgacaaggac gaaagccata cccagcgtca gggaaagaaa
     8581 ccataagatt ttgcggatga actcgttgat ttggtacagc gtttgcaccc attcggtatc
     8641 catagacgcg gattcgacca taggcagctt ggtaatgtcc cggtagattg cctgcatttg
     8701 ggcgggcgtg gttgccgggt cgggggtaac gataaagaca tccggcaggg ggttgccgtc
     8761 aagcatggaa atcagatttt ggtcgaggtt ggactgtaat tccgccaaac cgtcttcctt
     8821 gccgatgaag cggatgttgt cgagccgttt atcgcgcgtc agcaggctgc gtacggtatc
     8881 gctgtcgctt tgtgcggcgg cggtttccat atagacggtg atttgcggcg actcgttgag
     8941 tttgcccaac acgctttgcc cgctttggat gcccagatac ataaacagcg gcagggtcat
     9001 cgcgacggca agcattatga gggtaagcag tgtgccgaag ggttggcgca gaagctgttt
     9061 gagtgcggag cgcgcggatt cgacgtgcag cgagaagtag tggatgatgc tcatgcgagt
     9121 cgtcctttcg agaggcgcag gatgcggtgt ccgtagtccg ccatcagggt ttcgtcatgt
     9181 gcggcgacga tgacggtggt tcccgcttcg tggaaggttt tgaacaattc cataatatcg
     9241 agcgcgtagg cacggtcgag gttggcggag ggttcgtcgg caatcagcag gccgggctgg
     9301 tggacgacgg cgcgggcgat gcacaggcgt tgttgttcac cgcccgagag ggttacggga
     9361 tcgtccaatt ctcgtccttt caagccgact ttttcgatgg cgatgcgggc gcgttcttcg
     9421 gctttgcgcg gcggatagcc gataatccgc aggggcagga tgacgttttg caggacattg
     9481 cggtcgttga ggattttgtg gtcttggaac acgatgccga tgtgttggcg cataaagccg
     9541 atttggttgt cggacaatgt gccgaggtcc tgcccgttaa acaggacttt gcccatgctc
     9601 ggcttggtga tgcccgaaat cagcttgagg acggtggatt tgcccgaacc ggagtgtccc
     9661 gcgataaaaa tcatctcgcc tttgttgatt tggaagctga cgtttttcag ggcttcaaaa
     9721 ccgccgggat aggttttgga aacttgttcg aaacggatca taggaggtcc tgtaaggaag
     9781 ggcggcatat cgtttcgggc ggttgcgtcc gcagggatat gccgtctgaa caataggtaa
     9841 gacagtaccg attataaaga ttccgcctgt ttttagatag tggataagtt tggcggatac
     9901 cgaaggccgg gcgggaaaat gccgtctgaa gcggcttcag acggcatccg gcgtttaacg
     9961 gcattttgaa tgggcgagtt tgacggcttc ggtcaggctt ttctcgttca cctctcctgt
    10021 aatggtctgc ctgtatccgc atttcggggc ttcgacgacg gtaaagggca gtacgccgac
    10081 attgtttccg taggatttca taaagtttcg gctgttcgcc ccggtgtaac gccaaatcgg
    10141 gtagctgacc ggggtctgct tgagaaagtt gccgatattg tcggatgtgt cgagcgcgat
    10201 gccgaccata tcgacgctgc cttttttctg cgctttgtac catttggaca tggcgggcat
    10261 ctcttttcga cacggaccgc accaagtcgc ccataggttg gcgatgcgga cgggggcttt
    10321 gagcgattgc aggttttgcg gggtattgtc tttccatccg gccagttcgt ctgccgaggc
    10381 ggtatgtgcg gcaaatgtgg cggcaagggc gattgcggcg agattcaagc gtttcatcgt
    10441 tttgtccttt gtatgtcgat tcgggtattg taaaccgttt ggtacggttg gaggcggggt
    10501 attgagtaaa accctgcgta agccgataat aaggggattg tcgagaagta gggaaatgtc
    10561 atgtctgaaa tcaaaatttt ccacaatccg cgttgcagca aatcgcgtgc cgccgtgtcc
    10621 ctattggaag aacgcggcat tgctgccgaa gcggtcaaat atttggatac gccgcccgac
    10681 ttgtccgaat tgaaggatat tttcaacaaa ttgggcttgg aatcggcgcg cgggatgatg
    10741 cgcgtgaaag atgatttgta caaggaattg ggtttggaca accccgattt ggacaatgac
    10801 gcgctgctgc gtgccatcgc cgatcatccc gccctgttgg agcgtccgat tgttttggca
    10861 aacggtaagg cggctgtcgg cagaccgttg gaaaatatcg aagcggtatt gtgaacaaac
    10921 ggctgttttg cagccgcaac ggcttgcgct attacctgtt gggcggtttc tgtttgagtg
    10981 tttttccgct tttgctggtg tttgcctcat ccgtttgggc ggtttatcgg acgggcgggc
    11041 aggttttgcc gccgtatgtc cgggcggatg ccgcgctggt gttgggcgca gccgcgtggg
    11101 acaaacgccc ttccccggtt tttcgggaac gcatcaacca cgccatcgcg ctttaccaaa
    11161 gccgcagggt aggcaaaatc gtgtttacgg gagggcgaac caaaaaaggc tatatgaccg
    11221 aagccgaggt cgggcggcgg tacgcgctca aacagggggt tccggcgcgc aacatcctgt
    11281 ttgaaaacac ttcgcgcaac acttatgaaa acctgaacaa catccgtccg gttttgcgtg
    11341 ccaacggtat tgccagcgtg gtgattgtca gcgatcccta ccatttggcg cgggcggcgg
    11401 aaatggcgga ggatttgggt gtccgcgttt atatgtctgc cacgccgacc acgcggtttg
    11461 atgcgggaaa taagaaaaaa atctttatgc tgcaagaggg gtatgcgtta tccctctacc
    11521 gtttggaaaa atggggcagc cgctttttgg gatggctgtc tgattgaagc ggtagaaaat
    11581 gccgtctgaa acttatccgc aggcagggtt cggacggcat ttttgatgcc taagcacggt
    11641 aaaaacagta aaataaacgc tttcaaagat ttcggagcca ttatgaccgt caaaacccgt
    11701 ttcgccccca gccccaccgg ttacctgcac atcggcggcg ttcgcaccgc cttgttttcg
    11761 tgggcgtttg cccgccatca taaaggcgaa tttttattgc gtatcgaaga caccgacttg
    11821 gcacgttcca ccgccgaatc cgtcaacatc atccttgacg gcatgaaatg ggtcggtctc
    11881 gattacgaca acgccgacaa cgtggtgtac caaacccgcc gtttcgaccg ttataaagaa
    11941 gtcattgccg aactcttggc aaaaggcgat gcctactact gctattgcag caaagaagaa
    12001 ttggaagcga tgcgcgagaa agccgaaaaa gaaggcacgg cgacttacga ccgccgctgg
    12061 cggccggaag caggcaaaac cctgcccgaa attcctgcag gcgtgcaacc cgtcgtccgc
    12121 ttcaaaacgc ctttggacgg cgttaccaaa tggacagact tggtcaaagg cgaaatctcc
    12181 atccccaacg aagccctaga cgacctgatt attgcccgcg ccgacggtac gccgacctac
    12241 aacttctgcg ccgtggtaga cgactacgac atgggcgtta cccacattat ccgcggggac
    12301 gaccatgtga acaacacccc gaaacaaatc aacatcttga aagccatcgg cgcgaacctg
    12361 cccgaatacg ggcatctgcc gatgattctc aacgaacaag gcaaaaaaat ctccaaacgc
    12421 agcggcgata ccgtcgccat taccgatttc ggcgcaatgg gcattctgcc cgaagcgatg
    12481 ctcaactatt tggcgcgttt gggctgggcg cacggcgacg atgagttttt cacgacggaa
    12541 caattcatcg aatggtttga tttgaaagac gtttccccgt ccccaagccg tatggatttg
    12601 aaaaagctct attggatcaa cggcgaacac atcaaaatca cacccgacgg caaacttacc
    12661 gaactcgtca aaccgcgcct tgcgctgcgc gatattcatg aaaccgaaaa acctgcgttg
    12721 gaagacgtgt tggcactggt caaagaccgc gcccaagatt tgaacgcctt ggcagacgaa
    12781 tgcctgtatt tctacaaaaa acaagtccct gccgaagccg atgttgccaa acactgggac
    12841 gacgaagccg ccgcccgtat gctgcgcttc gccgaacgcc tcgaagggct ggaagactgg
    12901 aacgccaaag ccattcacga cctcttcaaa cctttctgcg acgaagaagg catcaaaatg
    12961 ggcaaactcg gtatgcccct gcgccttgct gtctgcggta cggcaaaaac cccgagcgtc
    13021 gatgccgtgt tggcattaat cagcaaagag gaagtgttga aacgcatccg tgcttaagtt
    13081 atccacaggt gcgggcaatg ccgtctgatg tcggaatgcg gcttcagacg gcattcagac
    13141 ggaagttgaa catgaaaaaa atattgtatt ttctgatgtt tgttttttct acaagcgtat
    13201 gggcaggggg tgcggaagac aatttgctca gcatccaatc cggttaccgt gctttactgc
    13261 aaaagcaaaa caatctggac ggaaaaatca tcgggatgca gtcggatttg gaagatgcgc
    13321 gccggcgttt gcaggcggct caggcagaca tcgcccgttt ggaagcggaa attcctgcag
    13381 caatggccca aaaagcccgg caggctgaag agatgaggca aatcggagtg cgtttggacc
    13441 atgcttggaa tgcggtttac ggcgcagggg aacgaaagcg tcgggggaat tgaccgatga
    13501 attccctctt tgtggacaac actgttttta ttacacggct gaaagccggg catatcggca
    13561 ggttggttca ggcgttgttt gaggagtggc acggatttga accgtggtct tctgtggata
    13621 agattcgtgc ctattacggc agatgtttga aggatgacga attgccgctg gcttttgcgg
    13681 ctgtggatga ttccggaacc ctgttgggtt cggctgcagt caagcggcat gatatggaaa
    13741 attttccaca gtatgaatat tggttgggcg atgtctttgt tttacctgaa tatcgcggaa
    13801 aaggcattgg caggaggctg gtcgcccact gcataggcgc agcgcgttcg ctggggataa
    13861 agttcttgta tctttatacg cctgatgtgc aaatatttta tgaatcattc ggctgggcgg
    13921 ttgtcgggcg acatttccat aacggggaat gggttacggt tatgcgtttg gatgtggata
    13981 aggtttaagc cgaatgggca gatgccgtct gagctgttcg gacggcattt tttatgtttg
    14041 gaaatttcct gtcagatata gtggattaac aaaaaccggt acggcgttgc cccgccccgg
    14101 ctcaaaggga acgattccct aagg
//
LOCUS       DAXDSD010000060        14038 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXDSD010000060 DAXDSD010000000
VERSION     DAXDSD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.060"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..221)
                     /locus_tag="ACFB2Q_001368"
     CDS             complement(<1..221)
                     /locus_tag="ACFB2Q_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918387.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRAALAAPVYIQTGATSFIPIPTEI"
     gene            386..1309
                     /locus_tag="ACFB2Q_001369"
     CDS             386..1309
                     /locus_tag="ACFB2Q_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC0918388.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFLG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETGCESEPRIPFEEKILFVDE
                     HLIVVDKPHFLPVIPGGRFLRETLLTRLRLRPELQHLNVEDITPIHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTKTDLPYPLDVVSRLVRGEKFFTTQEAEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(1388..2029)
                     /locus_tag="ACFB2Q_001370"
     CDS             complement(1388..2029)
                     /locus_tag="ACFB2Q_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC0918389.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(2084..2875)
                     /locus_tag="ACFB2Q_001371"
     CDS             complement(2084..2875)
                     /locus_tag="ACFB2Q_001371"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC0918390.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            3041..3116
                     /locus_tag="ACFB2Q_001372"
     tRNA            3041..3116
                     /locus_tag="ACFB2Q_001372"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3074..3076,aa:Thr,seq:tgt)
     gene            3258..4049
                     /gene="panB"
                     /locus_tag="ACFB2Q_001373"
     CDS             3258..4049
                     /gene="panB"
                     /locus_tag="ACFB2Q_001373"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC0918391.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKVHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            4172..5008
                     /gene="panC"
                     /locus_tag="ACFB2Q_001374"
     CDS             4172..5008
                     /gene="panC"
                     /locus_tag="ACFB2Q_001374"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC0918392.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            5335..7173
                     /locus_tag="ACFB2Q_001375"
     CDS             5335..7173
                     /locus_tag="ACFB2Q_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC0918393.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            7313..7894
                     /locus_tag="ACFB2Q_001376"
     CDS             7313..7894
                     /locus_tag="ACFB2Q_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC0918394.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGGTLGQLCQDRDGALAVDGKGNVYQAEGTE
                     DLSRQLVGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            7904..8749
                     /gene="ispE"
                     /locus_tag="ACFB2Q_001377"
     CDS             7904..8749
                     /gene="ispE"
                     /locus_tag="ACFB2Q_001377"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC0918395.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWQLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            8757..8832
                     /locus_tag="ACFB2Q_001378"
     tRNA            8757..8832
                     /locus_tag="ACFB2Q_001378"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8790..8792,aa:Gln,seq:ttg)
     gene            8845..8920
                     /locus_tag="ACFB2Q_001379"
     tRNA            8845..8920
                     /locus_tag="ACFB2Q_001379"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8878..8880,aa:Gln,seq:ttg)
     gene            9088..10071
                     /locus_tag="ACFB2Q_001380"
     CDS             9088..10071
                     /locus_tag="ACFB2Q_001380"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC0918396.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGKTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            10138..10710
                     /locus_tag="ACFB2Q_001381"
     CDS             10138..10710
                     /locus_tag="ACFB2Q_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC0918397.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(10836..12005)
                     /locus_tag="ACFB2Q_001382"
     CDS             complement(10836..12005)
                     /locus_tag="ACFB2Q_001382"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC0918398.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            12154..13680
                     /gene="ilvA"
                     /locus_tag="ACFB2Q_001383"
     CDS             12154..13680
                     /gene="ilvA"
                     /locus_tag="ACFB2Q_001383"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC0918399.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(13736..>14038)
                     /locus_tag="ACFB2Q_001384"
     CDS             complement(13736..>14038)
                     /locus_tag="ACFB2Q_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HFC0918400.1"
                     /translation="AWKLDAQYKWQEQTATGYIRPHEWQIAAEHETPMIRAEIEKIHA
                     VGALTHVLVKHGKQDVHITLAGSDAARYPIAEGKKLNLIPKQVYVFSQNELIEYSI"
BASE COUNT         3217 a         3295 c         4072 g         3454 t
ORIGIN      
        1 tgaatttcgg tcggaatggg gataaacgag gttgcgccgg tttgaatgta aaccggtgcg
       61 gcaagtgcgg cacggctgtt ttcgctattc agataggtaa gggaagtttc cagtttccat
      121 tttcccttgt cggtcattat gtcttcaatc gtcaagggca ggtcggcata agcggataaa
      181 ggcaggatgg cgggcaaggc gggcaaaaag atacgtttca tatttcctct tattgatatt
      241 aattcttatt taataatagt aatgaatatc cgataattct gtctaaacaa atttttaaat
      301 caaaaaaaac gatatatttg tttttatcaa ttatgttttg atatgccgtc tgaaaagttt
      361 gtgaaaaacg gttacaatcc gccgcatgaa aaaacgcaat aatcctcttc cgctgttgaa
      421 cggtgtcaaa cccagttatt tggtgctgcc gcatgaaaag cagttttacg ggctgccgct
      481 gctgcatttt ctgtgcatcc gctttccttt tttgggagcg gacgattggc ggaggcggtt
      541 gaacagcggt tttgtggtcg gttcggatgg tgcggcgttg gacgaacatt ctttgttcga
      601 gcccggtaag acgatgtttt attaccgtga aaccggttgt gagagcgagc cgcgtattcc
      661 gtttgaagaa aagattttgt ttgtcgatga acatttgatt gtggtggaca agccgcattt
      721 tctgcccgtc atccccggcg ggcgtttttt gcgggaaacg ctgctcacgc gtctgcgcct
      781 gcgtcccgaa ttgcagcatt tgaatgttga agacattacg ccgattcacc gcttggacaa
      841 ggatacggcg ggtgtgatgc tgctgtcgca caatcctgcc acacgcgggg cttatcagac
      901 gatgtttcaa aacaaaacgg tatggaaaac gtatgaggcg cttgcgccga caaagacgga
      961 tttgccgtat ccgctcgatg tggtttcgcg tttggtaagg ggtgagaaat tttttacgac
     1021 gcaagaggcc gaaggcgtgc cgaatgcgca cacgacggtc gaattgattg aaaacagggg
     1081 ggaattcagc ctttaccgcc ttacgccgca taccggcaag aaacaccagt tacgcgtgca
     1141 tatgatgggt ttgggtatgc cgctgttgaa tgacgcgctc tatcccgtgc cgtctgaagc
     1201 gggtagcgag gattatcgga aacctttgaa gcttttggca aaaaagattg cgtttgcaga
     1261 tcctttgtcg ggtcgggaaa gggtgttttg cagcgggttt tgcctgtaat gagagggaac
     1321 gcaaacccgt atcggcagat gccgccggat acgggtttga tattatcgat gctgtctgaa
     1381 aggggcttta tccgttcagg catacgggtg cgaagcggag gatggtgtcg cgcaggacga
     1441 tgagtttgcc gtgaaagaag tgcgtcgaac cggcgatggt aatgacgggc aaatcttgcg
     1501 gttctgccca tttcagggct ttttcgattt cgacgacctc atcttccgcg ccgtgtatca
     1561 tcagcgtttt ggcaacgttg ggaacggcgg acggctcggg gcggtcggta tagtggcaga
     1621 ctgccgcacc gatgagcagc aataaatcgg gtatgcgcac ttgtgcggca aatgtggcga
     1681 catagccgcc gaaggaaaag ccggataagg caaattccgg ggcttcggga tgttgggcgc
     1741 gggcatagtc gatgacggcg aggcagtctt gcgtttcgcc gcgtccgtaa tcatgtgtgc
     1801 cttcgctgcc gcctacgccg cgaaggttgg gaaggtagca gtggaagccg agcttgctca
     1861 atgctttggc ggcggtttgg atgactttgt tggtgttcgt cccgccttgg agggggttgg
     1921 ggtggttgat gacggcaaca ccgcgtgccg gtacttgttc ggacgggata tggatggttt
     1981 ctaaaatgcc ggcagggccg ggtatgtgta tggtttcggg tttcagcata atggtttcct
     2041 cttgattttc cgcatattcc gatgcagccg cgaagatggc ggcttaaagg tggaaatgct
     2101 tgccgttgtt ggggttggac gctttcaggc cggcgagcat gcggtggaag tccaatccgt
     2161 atgcgttgct cagtttgtcg gcacgttttt tgagtttgtc tatgttttgc tctttggggc
     2221 tgctttggaa ggccattacc gcgacattgc cgtggctttc ggcaggaagt tccaatacgc
     2281 gcccttcaaa aacgctcaac aaccgttcga tgaagcgttg atagcgtttg tcgccgctcc
     2341 accagttggt aacgaatatg ccgtctgaag agagtgcgtt gcggcagtct ctgaaaaacg
     2401 gttcttcaac cagcgtatcg atgatttgtt cgccgtcgaa tccgtccacc aagatgatat
     2461 cggtgttgtg gcggaagact ttgatgtatt ccgcaccgtc tgcttcgata atttcaaatt
     2521 ttccgccctc gaaaggcaac tcaaacaggt tgcgggcaat ggcgatgacc tgaggattga
     2581 tgtccacggc ggtttggcgc gtgtcgggca ggtaggtgtc tgtccagcgt gcaaacgaac
     2641 cgccgcccaa gccgatttgg gtgatatgtt gcggcaggcg ttccgcaaac agcagccagc
     2701 ccatcatcgc gcggctgtaa gagagcacca gctcggacgg gcggtcgagg ttcatcgagc
     2761 tttgaacggt gtcgctgccc aagtgaagcg aacggatatt gccttcttcg gaaataccga
     2821 cttcgggaaa gccggatttt gcaggacgca ggcggcggta gggatgtctt gccatcggtg
     2881 tgaacggtcg gtttgaaagg cggatatttt atcagaatgc cgatgcttgt tctgtttcaa
     2941 attaattttg ttttaaataa gatacttagt gtatttttat gagggggtcg aacaaatttc
     3001 ttgccaaagt gtgaagttgc ctgcataatt cgccttcttt gccggtatag ctcagttggt
     3061 agagcacctg acttgtaatc agggggtccc gagttcgact cttggtgccg gcaccagatt
     3121 tgacagcccg attgtgtaaa acagtcgggc tgttttgcat ttgcggcacg gtcggtggca
     3181 gggctgcggg catttcacta taatagcccg tttcaaactg aaaaggccgt ctgaaaaggg
     3241 cggggtaaca atatctgatg attactgtga acacactgca aaaaatgaag gcggccggcg
     3301 agaaaatcgt tatgctgacc gcttacgaat ccagttttgc cgcgctgatg gacgatgccg
     3361 gcgtggatgt gctgttggtc ggggattctt tgggaatggc ggttcagggg cgacagtcga
     3421 cgctgccggt cagcctgcgc gatatgtgct atcacaccga atgtgtagca cgcggtgcaa
     3481 aaaatgcgat gattgtcagc gatttgccgt ttggtgcata tcagcagagt aaggagcagg
     3541 cgtttgccgc cgccgccgaa ctgatggctg ccggcgcgca tatggtcaaa ctcgaaggcg
     3601 gcgtgtggat ggcggaaacg actgaatttc tgcaaatgcg cggaattccg gtctgtgccc
     3661 acatcggtct gaccccgcaa tccgtgtttg ccttcggcgg atataaagtt caggggcgcg
     3721 gcggcaaggc gcaggcgttg cttaacgatg ccaaggtgca tgatgaagcc ggggctgccg
     3781 tcgtactgat ggaatgcgtg ccggcggaac tggcaaaaaa ggtaactgaa actgtttcct
     3841 gtccgaccat cggcatcggg gcgggcgcgg attgcgacgg tcaggttttg gtgatgcacg
     3901 atatgctcgg tattttcccg ggcaaaacgg cgaaattcgt caaaaacttt atgcgcgggc
     3961 aaagcagcat ccaggctgcg gttcgggcgt atgttgccga agtcaaagcc aaaaccttcc
     4021 ctgctgcgga acatattttt gcagattgaa caaggtctgc tgccgatgcc gtctgaaagc
     4081 cgtttcagac ggcattttgt tttgccgtgc gcggcgcgta taatcggcgc gttttgtcgg
     4141 gcaggaagcc cgaaggataa ggattaccgt aatgcaaatc atacatacca ttcgagaact
     4201 gcgcacgtgg cgtgaaaata taggaaaggt ggcatttgtg ccgaccatgg gcaatctgca
     4261 tgaagggcat cttgcccttg ttcgcgaagc cagaaaacgc gccgacaacg ttgttgtcag
     4321 catattcgtc aaccgcttgc aattcggtca gggcgaggat ttcgacaaat atccgcgtac
     4381 tttgcaacag gatgcggaca aacttgccgc cgaaggcgtt gccgttgttt tcgcgcccga
     4441 tgagaaagaa ctctatccga acgtggaaca gcgttacaac gtcgaaccgc cccatttgca
     4501 aaacgaattg tgcggcaaat tccgccccgg ccattttcgc ggcgtggcga cggtggtgtc
     4561 caagctgttc aatatcgttt tgccggatgt tgcctgtttc ggcaaaaaag attaccagca
     4621 gcttgccgtg attaaaggtt tgaccgagga tttgaatttc gatatcgaaa ttgtccccgt
     4681 cgatacgggg cgggcggcgg acggtttggc actctccagc cgcaaccggt acttgagcgt
     4741 tggggaacgc gccgaagcac cgcgcctgta ccgcgagctg caggctgttg ccgaatcgct
     4801 gaagcagggc ggtttggatt atgcgggttt ggagcggcag gctgccgacc atttgaccgc
     4861 tgcgggctgg ctggtcgatt atgtggaaat ccgccgcgcc gatacgctcg aaatggcgcg
     4921 ggcgggagat aagaaactgg tggtcttggc cgccgcccgt ctggggacga cgcgtctgat
     4981 tgacaatgtg gaagtcggcc tgccttagcc cgccggagcc gggaatgccg cctgaagcgg
     5041 atttgcgttt cagacggcat tttgccgtcg gctgtgcttc ggaataatgt cggaaaatag
     5101 acggaatgaa ataaatttct ttttgttcaa caaattaaac tgtcaaacca aataataagg
     5161 caaggattgt ccggcatcgc atacaggcaa accgacgcaa tctcaaaaca cagccctgcc
     5221 ttcgggcggg ttttgcaaat ctacaaaccg tttggcttgt gataaagtga cgccttgtct
     5281 agatactcaa tatgccgtct gaaggttcgg acggatgtcg gataaaggat gattatgtta
     5341 cccgcccgtt tcactatttt atctgtcctc gcagcagccc tgcttgccgg acaggcgtat
     5401 gctgccggcg cggcggatgt ggagctgccg aaggaagtcg gaaaggtttt aaggaaacat
     5461 cggcgttaca gcgaggaaga aatcaaaaac gaacgcgcac ggcttgcggc agtgggcgaa
     5521 cgggtcaaca gggtgtttac gctgttgggc ggtgaaacgg ctttgcagaa agggcaggcg
     5581 ggaacggctc tggcaaccta tatgctgatg ttggaacgca caaaatcccc cgaagtcgcc
     5641 gaacgcgcct tggaaatggc cgtgtcgctg aacgcgtttg aacaggcgga aatgatttat
     5701 cagaaatggc ggcagatcga gcctataccg ggtgaggcgc aaaaacgggc ggggtggctg
     5761 cggaacgtat tgagggaagg gggaaatcag catctggacg ggttggaaga ggtgctggcg
     5821 caatcggacg atgtgcaaaa acgcaggata tttttgctgc tggtgcaagc cgccgtgcag
     5881 cagggtgggg tggctcaaaa agcatcgaaa gcggttcgcc gtgcggcgtt gaagtatgaa
     5941 catctgcccg aagcggcggt tgccgatgcg gtgttcggcg tacagggacg cgaaaaggaa
     6001 aaggcaatcg aagctttgca gcgtttggcg aagctcgata cggaaatatt gccccccact
     6061 ttaatgacgt tgcgtctgac tgcacgcaaa tatcccgaaa tactcgacgg ctttttcgag
     6121 cagacagaca cccaaaacct ttcggccgtc tggcaggaaa tggaaattat gaatctggtt
     6181 tccctgcgta agccggatga tgcctatgcg cgtttgaacg tgctgttgga acacaacccg
     6241 aatgcaaacc tgtatattca ggcggcgata ttggcggcaa accgaaaaga aggtgcgtcc
     6301 gttatcgacg gctacgccga aaaggcatac ggcaggggga cgggggaaca gcggggcagg
     6361 gcggcaatga cggcggcgat gatatatgcc gaccgcaggg attacgccaa agtcaggcag
     6421 tggttgaaaa aagtgtccgc gccggaatac ctgttcgaca aaggcgtgct ggcggctgcg
     6481 gcggctgccg aattggacgg aggccgggcg gctttgcggc agatcggcag ggtgcggaaa
     6541 cttcccgaac agcaggggcg gtattttacg gcagacaatt tgtccaaaat acagatgctc
     6601 gccctgtcga agctgcccga caaacgggaa gccctgatcg ggctgaacaa catcatcgcc
     6661 aaactttcgg cggcgggaag cacggaacct ttggcggaag cattggcaca gcgttccatt
     6721 atttacgaac agttcggcaa acggggaaaa atgattgccg accttgaaac cgcgctcaaa
     6781 cttacgcccg ataatgcaca aattatgaat aatctgggct acagcctgct ttccgattcc
     6841 aaacgtttgg acgagggttt cgccctgctt cagacggcat accaaatcaa cccggacgat
     6901 accgccgtta acgacagcat aggctgggcg tattacctga aaggcgacgc ggaaagcgcg
     6961 ctgccgtatc tgcggtattc gtttgaaaac gaccccgagc ccgaagttgc cgcccatttg
     7021 ggcgaagtgt tgtgggcatt gggcgaacgc gatcaggcgg ttgacgtatg gacgcaggcg
     7081 gcacacctta cgggagacaa gaaaatatgg cgggagacgc tcaaacgcta cggaatcgcc
     7141 ttgcccgagc cttcccgaaa accccggaaa taatgcaggc ccatcctttc agacggcata
     7201 aggtttgccg ggaagccggg gcattcgggc aaacggcacg cagttcgcac gcgttttgca
     7261 cggcacgccg cacccgtcgg ccggcaggat gacacccgtt aaggaaactc tgatgaaaca
     7321 caccgtatcc gcatcggtca tcctgctttt gaccgcttgc gcgcaattac ctcaaaataa
     7381 cgaaaacctg tggcagccgt ccgaacacat cagcagtttt gcagcggaag ggcggttggc
     7441 agtcaaagcg gaagggaaag gttcgtatgc aaatttcgat tggacatacc aaccgcccgt
     7501 ggaaaccatc aatatcaaca cccctttggg cggcacgctc ggacagttgt gtcaagacag
     7561 ggacggcgca ttggcagtgg acggcaaagg aaatgtctat caggcagagg gtacggaaga
     7621 cttgagcagg cagctggtcg gtttcaaact gccaatccaa tatctgcata tctgggcgga
     7681 aggcaggcgt gtggcgggcg cgccttaccg catccgttca gacggcatat tggaacaata
     7741 cggttggaca atcggcagaa ccgccgacag tggggggcaa gtccgaacgt tgcaactgaa
     7801 taacggaaat ttgaacatca ggctggtttt caccgaaatt ggaatgccgt ctgaaaccga
     7861 aaccccggaa cgctgtgcgg cgcgcacgag ataaggcgga cagatgaata ttgcggacgg
     7921 acggcaggcg tttcccgcac ctgcaaaact gaatctcgat ttgaggatta ccggcaggcg
     7981 ggaagacggt tatcacaata tcgaaagcat attctgcctg atagatttgc aggataccgt
     8041 atatttgaaa ccgagggacg acggcaaaat catcctgcac aatcctgtcg gcggaatacc
     8101 gcaggaagcc gatttgagct accgtgccgc atcgttgctg caaaaatatg cgcgcaacct
     8161 tgccggcgtg gaaatatggc tggacaaaaa aatcccgacc ggcgccggct tgggaggggg
     8221 gagttccgat gccgcaacgg ttttgctggt gttgaaccgt tggtggcagt gtggtttgac
     8281 gcagtggcag ctcattgatt tgggtgcggc tttgggtgcg gacgtaccgt tttttatttt
     8341 cggaaaaaat gcttttgcaa gcgggatagg tgaaaaactg ataggaatgg atattccgaa
     8401 acagtggtat gttattgtca aacctcccgt ccacgtttcc accgcaaaaa ttttcacata
     8461 cgaaggcttg acacgggatt ctgcctcaag cataatgccg actttccaaa acctgcaacc
     8521 gttcagaaac gatatgcagg cagtggtatt taaagaatac cctgaagttt ggaaagctta
     8581 ttccgagttg tcgaaatacg gttctgcaat gatgaccggc tcaggagcct gtatatttgc
     8641 agcatttcaa gctaggaata gcgcatacaa tatataccga caagtttcag gtttgtacga
     8701 ggcatatttg gcagagggtc tttcaaaaca tcctttgttg tccgtataaa cattgttggg
     8761 gagtcgtcaa gcggttaaga cactggattt tgattccagc atgcgaaggt tcgaatcctt
     8821 cctccccagc caagccaaac aagttgggga gtcgtcaagc ggttaagaca ctggattttg
     8881 attccagcat gcgaaggttc gaatccttcc tccccagcca gacaaaagcg tgtaagcctg
     8941 cttacacgct ttttatttca tagaaataaa aatattgaaa cgcctttgtt tgtgtcggat
     9001 gttgcaggta taatgccggg cttggtacaa gcggagggaa gcattgtgtt ttctgagcgg
     9061 aagttaaaca taaaatcagg tgagaatatg gctgcgtacg acagtttgat ggtatttaca
     9121 ggcaatgcca atcccgaatt ggcacaacgt gttgtcaggc atttggacat ttctttgggc
     9181 aatgcttccg tatccaagtt ttcagacggc gaagttgccg tcgaactgtt ggaaaacgta
     9241 cgcgggcgcg acgttttcat ccttcagccg acctgtgcgc cgaccaatga caacctgatg
     9301 gaaatcctga cgatggcgga tgcactgaag cgtgcttcgg caggtcgtat taccacagcc
     9361 attccgtatt tcggctatgc gcgccaagac cgccgtccgc gttccgtccg cgttccgatt
     9421 tctgccaaac tggtggcaaa tatgctgtat tcggcaggga tcgaccgtgt tttgactgtc
     9481 gatttgcatg ccgaccagat tcaaggtttc ttcgatattc cggtggacaa tatttatgcc
     9541 actccgattt tgttgaacga catcaagcag cagcggattg aaaatctgac cgtcgtcagc
     9601 ccggacatcg gcggtgtcgt ccgcgcccgc gccgttgcaa aatccctgaa tgccgacttg
     9661 gcaatcatcg acaaacgccg cccgaaagcc aatgtggcgg aagtcatgaa catcatcggc
     9721 gatattcaag gcaaaacctg tctgattgtg gacgatatga ttgacactgc aaatacgctg
     9781 tgcaaagccg ccgtcgcctt gaaagagcgc ggtgcagaac gtgtcttggc ttatgccagc
     9841 cacgccgtat tctccggaga ggcggtcagc cgtattgcct catccgaaat cgaccaggtg
     9901 gtcgtaaccg ataccattcc tttgtctgaa gcggctaaaa actgcgaccg tatccgtcag
     9961 gtaacgattg caggtttgtt ggccgaaacc gtccgccgca ttagcaatga agaatccgtc
    10021 tcatatcttt tcaatgaaga agtgatgaca ggcagcatgt tgctgccgta accccgatgc
    10081 cgtcttaagc tggtcgcggc cgatgacggc gattttacct aacttggagt atttaacatg
    10141 acttatgaaa ttcaagcctc tgttcgtgaa gcacaaggca ctggtgcgag ccgccgcctg
    10201 cgtcgcgaag gccaaatccc cggtattctg tacggtgaag gtcaagagcc tgttgccatt
    10261 gccgtagatc acaaaaccgt attttacgca ttggaaaaag aatctttcca tactgcgttg
    10321 attaaattgt ctctgaacgg cgaaaccaaa gacgttatcg tccgcgattt ccaaatgcac
    10381 ccgttccgcc gcgaagtcca acacatcgac ttccaagctg tgaaagccga tcaacttgta
    10441 cgcatccgcg ttcccctgca cattgttaac gctgaaaatt ctcaagccgt taaattgcaa
    10501 ggcggtcgcg tatctctgct gaacacggct gttgaagtgc ttgcgttgcc tgccaatatc
    10561 cctgctttct tggatttgga ttgtgccgaa gtggttgccg gcgacatttt gcacttgtca
    10621 gacatcaaac tgcctgaagg tgtagaaagc gtttccctga aacgtaacga aaatctggct
    10681 gttgctaccg ttaccggtaa aaagcgctaa cttacttcca tcagcagggc ggcgcgtatg
    10741 caatacgtac cgccctgttg ttttatgccg tctgaaccgt gtttcagacg gcatttcttt
    10801 atttgttgga aaagcgggat atttgaaacg gcagattact gccctgtcag acgcgtccaa
    10861 agcctttgcc accggcttct tttttctacg ttttccagtg cgacgatttc tttttcggca
    10921 atggtatgtc cgttttgcct gattttgatt tttcctaaaa tctgcccttt ttttaccggg
    10981 gcgggaatcg gctgtatggt ttccaaaatc tgttctgcca ttttcgcttc tttatgtggc
    11041 agagtgatgt aggcttcttt gaggaagcct gcgcggacgg tttttttgct gcctccggaa
    11101 atttggattt gggcaacggt tttgcctttc ggatatattt tgggcgtatc gaaggcctgc
    11161 aatgcccggt tcagcagctt gctgttgtcc gatgcgcggg tttccgccga ttccgaacct
    11221 agtgtgatga caaggatgtg cctgccgttg ccggagtatg acacggcaag gttgtagccg
    11281 ccgctttctg tgtgcccggc tttcaggccg tttacattgt tgtccctata taaaaggata
    11341 ttgcggttgt tttgttctat gttttcaaac ttgaacgatt tgatggaaaa cagcgggtaa
    11401 tattccggaa agtcgcgcat caatgcttca gacagcaggg cgaggtcttt ggcggtggaa
    11461 acctgtcctt ctctacccaa gcctgtcggg tttttgaata cggtgttctt catgcccaag
    11521 cgtcgggctt ctttgttcat ttgttgcaca aaattttcaa tcgagccgtt gcccagccgg
    11581 tcggcaaggg ttagggcggc atcgtttgcg catagggcaa tcatgccttt taagagtttg
    11641 tcggtactga ccgtatcgcc gggacgtaca aacattctgc ttccttctga agcccatgcg
    11701 gattcgggta tttttaagtt ttcttcagat tggatatttc ccgatttcat gtttttgaaa
    11761 accaaatatg cggtcatcag ttgggttagt gccgccggtt cgacaggggt attggtgttt
    11821 ttggcggata aagtctgcct gctttgaagg tcgataacga tgtgtgccgc cgtgagggtt
    11881 tcgggtgttt ggagcgtggg ggcggcgtgt accgtcggtc tgttgggcgc gggcgatgca
    11941 gccgttgcgt gagaaacgcc taagatgatg ggaagaagga cgggcaggat tttatgtgct
    12001 gtcatgaaat attctaaatt gtgtgagtgt ttcagcctgc cgattatacg cttagggtgt
    12061 ctgatcgggc ggatttttct tgctttcgcg ccgtcttggg cgtatggttt tgggttttgc
    12121 gattttaata aaccgattat cccgcattga attatgaaca cgccccttcc ttattccgat
    12181 tacctcatcc gcatcctgac ggcatctgtc tatgatgtag cggtcgaaac gcctttggaa
    12241 ccggcacgca gcctttctgt acgtttgaaa aacaacatcc ttttgaaacg cgaagatttg
    12301 cagccggttt tttcgttcaa aatacgcggc gcgtacaaca aaatgtccaa gttgccgaaa
    12361 gatgcgctcg cttgcggcgt gattgcggca agcgcgggca atcacgcaca aggcgtggcg
    12421 ttgtccgcac agcgtttggg ctgccgtgcc gttatcgtca tgccggagac tacgccgaaa
    12481 attaaagtgg atgcggttaa aagccgtggc ggcgaggtgg ttttgcgggg tgtttcatac
    12541 aacgatgcct acgattatgc gatggagctg gcggaacagg aagggctgac ctatatcgcg
    12601 ccgtttgacg atcccgatgt gattgcggga caggggacgg tggggatgga aattgtcagc
    12661 cagcatcccg atccaatccg cgccgtattc gtaccgatag ggggcggcgg tttggcggcg
    12721 ggcgtggcgg catttatcaa acaggttcgt cccgaaatta aagttatcgg cgtacagacc
    12781 aacgattcct gctgtatgaa gcagtcggtc gaagcaggcg aaatcgtcca tttgaaagat
    12841 gtcgggctgt tttcagacgg tacggcggtc aaagtcgtcg gaaacggaac cttccgcctc
    12901 tgcaaagaac ttttggatga aatcattaca gtcgataccg atgcggtttg cggcgcggtc
    12961 aaggatattt tcgatgacac gcgcagcatt accgagccgg ccggcgcgtt ggcgttggcg
    13021 ggtctgaaag cctatattgc ccgagaaggt gcggaaaacc aaaccctgat tgccgttacc
    13081 agcggtgcga atatgaattt tcaccgtttg cgccacgttt cggaacggag cgaattgggc
    13141 gagggcaacg aaggtatttt tgccgttacc atccccgaag aacgcggcag cttccttaag
    13201 tttgtcaata tattgggaaa taggaatatt accgagttca actaccgcta cggcgacgat
    13261 gaaaaagcgc atatctttgt cggacttcaa gcggcaggcc cgcaggattt ggcggttatc
    13321 ggcagccggt tggatgaggc gggattgccc aatgtcgatt tgaccaataa tgagattgcc
    13381 aaaatccata tccgctatat ggtcggaggg cggacggaca aagtagggca cgagcgtctg
    13441 gtcagttttg agtttccgga gcgtccgggc gcgttggcgc gctttttgaa ccatatgcag
    13501 ggcggttgga acattacgct cttccattac cgcaaccacg gtgcggatta cgggcggatt
    13561 ttggtcggta tcgacgtgcc gccgcacgat gccgccgcat ttgacggttt cttggaaagt
    13621 ctgggataca gctatcacga ggaaacgcaa aatgccgcgt acaagctgtt tcttgcctga
    13681 cgtttgacac gcaatgccgt ctgaaagcct ttcagacggc attgcgcttt cagggttaaa
    13741 tcgaatattc aatcaactcg ttttgagaaa aaacataaac ctgtttcgga attaagttta
    13801 attttttgcc ttcggcgatt gggtagcgcg cggcatcgct gccagccagt gtgatatgca
    13861 cgtcttgttt gccgtgtttc accaaaacgt gcgtcaatgc gccgacggcg tggatttttt
    13921 cgatttcggc acggatcatc ggggtttcgt gttcggcggc gatctgccat tcgtgcgggc
    13981 ggatatagcc ggtggcggtt tgctcctgcc atttgtattg cgcgtccaat ttccacgc
//
LOCUS       DAXDSD010000061        13910 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXDSD010000061 DAXDSD010000000
VERSION     DAXDSD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.061"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            1..635
                     /locus_tag="ACFB2Q_001385"
                     /pseudo
     CDS             1..635
                     /locus_tag="ACFB2Q_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            638..1736
                     /locus_tag="ACFB2Q_001386"
                     /pseudo
     CDS             638..1736
                     /locus_tag="ACFB2Q_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1748..2283
                     /locus_tag="ACFB2Q_001387"
                     /pseudo
     CDS             1748..2283
                     /locus_tag="ACFB2Q_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            2285..2927
                     /locus_tag="ACFB2Q_001388"
                     /pseudo
     CDS             2285..2927
                     /locus_tag="ACFB2Q_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            2948..3307
                     /locus_tag="ACFB2Q_001389"
     CDS             2948..3307
                     /locus_tag="ACFB2Q_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC0918401.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSVYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            3399..3474
                     /locus_tag="ACFB2Q_001390"
     tRNA            3399..3474
                     /locus_tag="ACFB2Q_001390"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3432..3434,aa:Asn,seq:gtt)
     gene            complement(3972..4883)
                     /gene="crgA"
                     /locus_tag="ACFB2Q_001391"
     CDS             complement(3972..4883)
                     /gene="crgA"
                     /locus_tag="ACFB2Q_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC0918402.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            5122..5700
                     /locus_tag="ACFB2Q_001392"
     CDS             5122..5700
                     /locus_tag="ACFB2Q_001392"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC0918403.1"
                     /translation="MNILLLDGGKAFGHSHGGLNHTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYMDEVFTGGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFIGLS
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(5908..7293)
                     /gene="pntB"
                     /locus_tag="ACFB2Q_001393"
     CDS             complement(5908..7293)
                     /gene="pntB"
                     /locus_tag="ACFB2Q_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC0918404.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLKGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(7373..7756)
                     /locus_tag="ACFB2Q_001394"
     CDS             complement(7373..7756)
                     /locus_tag="ACFB2Q_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC0918405.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(7760..9298)
                     /locus_tag="ACFB2Q_001395"
     CDS             complement(7760..9298)
                     /locus_tag="ACFB2Q_001395"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC0918406.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGSGDGCAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSARPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            9587..10420
                     /gene="serB"
                     /locus_tag="ACFB2Q_001396"
     CDS             9587..10420
                     /gene="serB"
                     /locus_tag="ACFB2Q_001396"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC0918407.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFGQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVAYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            10484..11707
                     /locus_tag="ACFB2Q_001397"
     CDS             10484..11707
                     /locus_tag="ACFB2Q_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC0918408.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIRHTAYGYDADGAY
                     ISFREGVAQAFGMRRVAMLTLCGAIAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAAFFRRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQYSLELTAVKWAVVLMALAAGVYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            11868..13448
                     /gene="purH"
                     /locus_tag="ACFB2Q_001398"
     CDS             11868..13448
                     /gene="purH"
                     /locus_tag="ACFB2Q_001398"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC0918409.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQNLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPAMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDINR
                     INRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGIRHFRH"
BASE COUNT         3361 a         3459 c         3710 g         3380 t
ORIGIN      
        1 acgaccgtgc catccaaact tccgaaaaac ctgcaaacct gattatcgat gcgttgaaac
       61 tcgattacca acacgcggac atagaccgtt ttgccggaca tttgtggaaa ctttaccaaa
      121 cgttgggcaa ctacggcagg caggttaaag agcggatgct gggttagggg tacagcctgg
      181 gttaccatga agttttcgat gatttggaaa agggggcgaa cgatcggcaa atcaaagaca
      241 tcggtatgag gcacgggtat ctgccgaaaa atatacagaa attcaaagcg caacatcggg
      301 atttggtgct tcaagattct tcgttgatca acactggttc gtctccggca gacgatgcgg
      361 ttaaagaagt agagtcgttg ctgatgtatg gtcagattga agcggcaatg gatgtgttgg
      421 agcaggcggt attgaaatat cccgacgaat cccagcttta tattacgttg atcgatattt
      481 atgaacgtac tgaagattgg gataggttgg ggcagttttt aagggtattg agggaacgtg
      541 cggacaggct tcctgaagag gtcgttatgc tgatgagccg gctgctgcag cgtatgaatc
      601 aaaatattaa aaaaataaaa cggtacggaa aataaaaatg gaagttcaac tgccgaaaat
      661 taaaacagta cgcgtaatgt tggcggggat gacggcgcag caggaatccg ttttcaaaat
      721 ggcattcaaa atgcacaata ccacccgtta tgaaacagta tccccttcag acggcagtgc
      781 cgtgcccgat ttggttttgg cggataccga tgccgagggc ggttttgaac tttggaaaga
      841 gcttgccggg cgttataagg ctatacccgt cgccgtctgt tcggagaaag ttcccgattc
      901 tgaagttccc tacctgccca aaccgattcg gtttgaaaca ttgtttccta tgctccgcaa
      961 gttattgcag ggcgagaatg tttatgggaa atcgtttatt gcacccgcag accggtcggc
     1021 gaaaaacaac gggaatgtgc agcgtacggt tacgatacgc cagtttaacc cgaataaagg
     1081 attattgggg gcgttgcggt ttgcggaaaa gaacaggcag gacatcgcta tcttgcatgg
     1141 aaataagccg gtccttaatt gttttcccct cgatacaacg ggttttgctg acagaaagtg
     1201 tgtaaaaact cgaagaattg tgcaaagacg aaaatttgca ggtcagttgc aagactgttc
     1261 ccgataactc gcaatggcgc gaaaaggcta aagtaggcat tatgtcctgt atgtggcagt
     1321 tttccatttg gacggcgcag gacaggttga tttatccgat ttttcccgat actccgttta
     1381 cgttgaaatc ttggccaaac ctgacccggt aggcaaatgt gccggggtcg atacgcttgt
     1441 cggcattttt gaccaaggca tccgtcaacc ttaacgtgtt gtataaagtg atgcctttaa
     1501 acctcaatga tattctgaat tatcttgcgg caacctatac aaccgggttt ttgtcggtag
     1561 atttgaaaac ggtttcacaa caggcatact ccgatatggc ggataaaata aatatcgggg
     1621 ccgattctgc ttctgatagt gaaatgatga aaaaagcgga aaaaatcacg acaccatccc
     1681 aatcccagtc gcgcggcctt ctgcaaagac tgatgaaaaa actgttgggc agctaagagg
     1741 cggagagatg agagaaaata aaattatttt cacaggacct gtcggcgtag ggaaaaccac
     1801 tgccattgcg gctatttcgg acgaagcact cgtccagacc gatgcttccg catccgacat
     1861 gactttggat aggaaaagga atacgacagt ggcgatggac tacgggccat cagcttggat
     1921 gaggatacca aagtccattt atatagtacg cccggtcagg aacggttcaa ctttatgtgg
     1981 gaaatcttaa gccaaggcag tatgggtttg gtattgcttt tagataatgc ccgaaccaac
     2041 tcgttgaaag atttggaatt ctttttacat tcgtttcgag ggctgctgga gaaggcatcc
     2101 gtcgttgtcg gtattaccaa gatggatata cgctctcagc ccggtatcga cgtgtatcac
     2161 aaatatcttg caaaacataa tcttaatgtt ccggtttttg aaattgatgc ccgtaaggaa
     2221 gatgacgtaa aacaattggt tagcgcaatg ttattttcta ttgatccggg actggaggtt
     2281 taatatggaa tcaacacttt cactacaagc gaatttatat ccctgcctga ctcctgccgg
     2341 tgcattttat gccgtatcca gcgatgcccc cagtgccggt aaaactttgt tgcgcagcct
     2401 gttgaaagcg gatgcggacg aagtggtcag cagtgagaag ctgctcgcgg gcggacaccg
     2461 ccgacatcga taccgctttg aacctgttgt accgtttgca aaaactcgaa ttcctctatg
     2521 gcgatgaaaa cggtcattca gacggcatca atttgtcgga cgagcaattg ccgttgctga
     2581 tggaacaatt gtccggcagc ggtaaggcat tattggtcga tcggaacggt ctgtatcttg
     2641 ccaacgccaa tttccatcat gagtcggcgg aagagttggg gttgttggcg gcagaagtcg
     2701 cacagatgga aaagaaatac cggctgctga ttaggaacaa cctgtatatc aacaataacg
     2761 cttggggcgt ttgcgatcct tccggtcaga gcgaattgac atttttccca ttgtatatcg
     2821 gttcaaccaa atttattttg gttatcgccg gcattcccga tttgagcaaa gaggcatttg
     2881 ttactttggt aaggatttta taccgccgtt acagcaaccg cgtgtaaaac ttgggacagg
     2941 agggattatg cagcaattat tgatttcaat ccttgaagat ttaaataata catctacgga
     3001 tattatcgcg tctgccgtta tctcaaccga cggattgccg atggcgacaa tgcttccttc
     3061 acatttgaat tcggacaggg taggggcgat ttctgccact ttgcttgctt tggggagtcg
     3121 ctcggtgcag gaactcgcct gcggggaatt ggaacaagtg atgattaaag gaaaatcagt
     3181 ctatatcctt ttaagtcagg cgggtaaaga tgccgtgttg gtgctggtgg caaaagaaac
     3241 cggcagactt ggtttaatcc tattggatgc caaacgtgcg gcaaggcata ttgcggaagc
     3301 catataacat ataaagattg cgggcttgca gataagtgca atcgattgtc aatttatatt
     3361 gacacgttcg gtatttctgt tttattattc gcgtttactc cccgatagct cagtcggtag
     3421 agcgacggac tgttaatccg caggtccctg gttcgagccc aggtcgggga gccaaattcc
     3481 aaaaccctct aagtattttc ttagagggtt ttgttttacc ggcggtcaga aacgcatttt
     3541 tgagatgatt gttttgagat ggaataaaat ctttgcaaaa ttcctttcgt gatggttatg
     3601 aaaaataaat ttctaaagat ttaattggtt atttttataa ttttgaaatt tatcggtttt
     3661 gtaaaggtct cgggataagc aatttcggtc ttcggcgcgt cttttacgcc aaacccataa
     3721 gaattttgag ggtaatcttc atcgttttgc ctgaattgcc gacgcaaagc gatttatcgg
     3781 cggttgtgtt tgagcgtttg attgcagctt gcccaatctc aaatatttct aaataccaat
     3841 tgaaattcaa tattttataa aaacactgat ttggaacgaa cagatttata aaaattccgt
     3901 gtatccttga gtaagggagt tgattgaaaa gacttgtttg attgatgatg aagaataagt
     3961 taattaatgt cttatttggt attcgttcta ttcatatttt ttcccagttc cttcactaaa
     4021 aaatccaaaa atacgcgtaa gcggaggttg acggctttat cgctgtaata aacagcatta
     4081 aaggggtgtg ttttattgga ggtttgttcg gcgaataggg gaattaactt tccttcagtg
     4141 atgtcgttgt caaccaaaaa atctgataag caagcaatac cgcaacttga aaggcacaac
     4201 gagcgtaaga tttcaccgct gctggcggta aagtgcggtg aaattttata gggatttccc
     4261 tgcgcatcta aaaccgccca tgtatttaga gaaccgggtt ctgtgaagcc taaacattga
     4321 tggttggcaa gatcttctgc agattgtggc gtgccgtgtt ttgctaaata ttcaggactg
     4381 gcgactacgc ggaagtggct gtcaaacaga tggcgtgcac gcagcccgga atcgtccaat
     4441 tctccggccc gtaaggcaat atcgactttg cgttcaatca gattgatata gccttcggaa
     4501 gaaacgagcg aaagtcggat atgcggatag cgttcgttga attttgctgc cagcggcgcc
     4561 agcagatgca gcaccatcgg catcgcggaa tccacgcgca acacgccttg cggtacttcg
     4621 tgcactgcca gcatttcggt ttccgccgct gccatttctt gcaggattct ctgcgcgcgg
     4681 cggaaatatt gcgcgccttc ttccgtcaga ttgagttgcc gcgtggtgcg gttgagcagg
     4741 ttcacgccca acttttcctc cagccgtttg acgatgcggc ttacggcaga atttgccatc
     4801 tccaactgct ccgccgcacg gctgaagctg ccgctttcca ccacttgaac aaatacggtc
     4861 agttcttctg aattggtttt catcgtgttt ccttttcggt tggaacccca ccctttaggg
     4921 cggcaggatc agactttatt tgggaagggc gtaacccctt cccaatcagg acggcacata
     4981 gggagacgat ttatgtgtca tcccgtgtgt tgaaacatct gattattcca tttgacgcaa
     5041 aagtgttttc ttatttttgc acttttaaat cataaagtaa aacggtacaa tacattcatc
     5101 aattcacaaa cgaggtaaca aatgaatatt ttattattag acggcggcaa agcgttcgga
     5161 cattctcacg gcgggttaaa ccacacgctt cacaaaaaag cgaaagaagt tttgaccgcg
     5221 ctcggacaca atgttcaaga aaccgtgatt gatgccggct atgatgttga ggcggaaatc
     5281 gaaaaattcg tttggatgga tgctgtgatt tggcagatgc cgggctggtg gatgcacgag
     5341 ccttggacag tgaaaaaata tatggacgaa gtctttaccg gcggacacgg caaactctac
     5401 caaagcgacg gcagacaccg cgtcaatccg actgagggct acggcacagg cggcttgttg
     5461 caaggcaaaa aacatatgct ttcactgact tggaatgcgc cgattgaagc ctttacccgc
     5521 gaaggcgatt tctttgaagg caaaggcgtt gatgttttgt atatgcactt ccacaaagcc
     5581 aacgagttca tcggcttgag ccgcctgccg acattcttat gtaacgatgt ggttaaaaat
     5641 ccgcaagtgg aaaaatactt ggcagattat caggcacact tggaaaaagt gttcggctaa
     5701 ttaaaaatcc atcttcaaca cggagatgga ttttgtttgt ttcgttgatt ttgtgtcagt
     5761 ttcagatgtg ggtgcttatc cggacgggtc gtctgaaaat gtttgcccca atgcaaaaaa
     5821 atcactgcaa accttcacaa acggggctgg tagtgatttt ttcaaatcaa acagattgaa
     5881 aacctgcgcc gaattgttca gacggcatta ttttttcaat tccgccaaaa tcccgtcaac
     5941 ggttttcttc gcgtcgccga aacacatcac gctgttttcg ttgaagaaca gcgggttttg
     6001 tacgcctgcg tagccggtgt tcatcgagcg tttgaatacg acgacttctt ttgccttcca
     6061 tacttctaaa accggcatac ctgcaatcgg gctgttcgga tcggtttggg cggcagggtt
     6121 gacggtatcg ttcgcaccaa tcaccaagac cacatcggtt tcggggaagt cgtcgttgat
     6181 ttcgtccatt tccaaaacaa tgtcgtaggg gactttggct tcggcgagca gtacattcat
     6241 atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttcga tgccgttttt
     6301 acgcaaaagc tcggtgattt cggcaacggg gtattgtgcc tgcgccactg ccatgccgta
     6361 gcccggggtg ataatgacgc tgcttgcgcc tttcagcatt tcggcaacat cggcagcttt
     6421 gacttctcgg tattcctcta tctcttggct gccggaagac gatatgccgc tgtcgctgcc
     6481 gaaaccgccg gcaatcacgg aaacaaacga ccgattcatg gctttgcaca taatgtagga
     6541 cagaattgcg ccgcttgagc cgaccagcgc gccggtaacg atgagcaggt cgttggagag
     6601 cataaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatag atacgaccac
     6661 gggcatatcc gcgccgccga tggaggcaac caagtgccag ccgaatgcga gggcgatcag
     6721 ggtcataatc agaaggatga agccgctgcc gtcaatgccg acaaatacga gcagcaacac
     6781 aaacgatacg gcgagcgcca gtgcgttgag cttgtgtttg gcgggcagtt gcagcgggct
     6841 gctgctgatt ttgccgttga gtttgccgaa tgcggccagt gagccggtaa aggttaccgc
     6901 gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat cgtgcgaaac
     6961 gttgcccggc tcgatgtagc tgttgaagcc gaccaaaacg gctgccaagc cgacgaagct
     7021 gtgcagaagt gcaatcagtt cgggcatttc ggtcatttct acttttttgg ctttgtggat
     7081 gccgattgcc gcgccgatga gcatggcgat gataatccag cccagtccgt gggtattgtc
     7141 ggaaaagacc gttacgaaga gggcgaccgc cataccggcg ataccggaat agcagccccg
     7201 tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa ttgcggcaac
     7261 gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt cgatgatttg
     7321 ttttcaatgc cgtctgaaaa attgacgttc gtgttttcag acggcatctg tttcaagcag
     7381 ccgcgacaaa cagtccgacg gtgcaggcaa atccgcccgc ccacatcagc gagcgcaatg
     7441 ctgctttgtc tgcgatgtag caccagataa aggcgaggcg gaacaggatg aacaatccgg
     7501 caagcgtgtt gacggttgct tgtccggcat tgccggttgc gtgtgccgtc aaaacggcgg
     7561 cggcaaacgg tgcaaaggct tcaaaaccgt tttgctgcgc ggcgtgggca cgggcggctg
     7621 cgccttgcgt atgtgccaga aaaccgcgag gattgtggtt gtctttaaac cggaatccgc
     7681 ctgctttttt ggcatacgcc gcacaaaaaa gcggcaatag gcaggcaatc agaatgcacc
     7741 aataggcgaa agtcatggct taccctttct taaacatatt cagcatacgc cgtgtaaccg
     7801 caaagccgcc gaagatattg atgccggcaa tcaggatggc aacaaacgac agcagcgaaa
     7861 cgaagccgtt gccctgaccg atttgcagca gcgcgccgac gacgatgatg ccggagatgg
     7921 cgttggttac cgacatcagc ggtgtgtgca gcgagtggct gacgttccaa acgacatggt
     7981 agccgatgac gcaggcgagg acgaagacga taaagtggtt caagaatgct gcgggtgcga
     8041 ccgcgccgac ccacagcacc aatacggcgg cgatggcggc gggcgcgagt tttttccaca
     8101 ggggaacagg tttcggctcg ggcttggcgg caggcgcggc tttttcagac ggcgtttgct
     8161 gcggccgggc ggaaacctga atcggcggag gcgggaaggt gatttcgccg tcgcgggtaa
     8221 cggtcatatt gcggataatc acgtcttcga agtccagcgt gatttcgccg tctttgttcg
     8281 ggcttaacag cttggtcagg ttcaccaagt tggtggcgta aagctgggaa gactgtccgg
     8341 caaggcggtt tgccatgtcg gtgtagccga tgattttcac gccgttgccg gttacggaca
     8401 attcgcccgg tcgggtgagt tcgcagttgc cgcccgtcgc cgccaaatcg acgatgacgg
     8461 atccggattt catgctttcc accatttctt tggtaatcag cttgggagcg ggtttgcccg
     8521 gaatggcggc ggtggtgatg atgatgtcca cttctttcgc ctgttcggca aagagcttca
     8581 tttcggcggc gataaattcg tcgctcatca ctttggcgca gccgtctccg ctgccgcccg
     8641 attcttgcag gaagtcgagt ttcaggaact taccgcccat cgattcgatt tgttccgcca
     8701 cttccaagcg ggtatcgaac gcgcgcacca ctgcgccgag cgaatttgcc gtaccgattg
     8761 ccgccaaacc cgccacacct gcaccaatca ccaaaacctg cgcaggcggc actttgccgg
     8821 cggcagtgat ttgaccggtg aagaaacggc cgaaggcgtt ggcggcttca atcacggcgc
     8881 ggtagccgct gatgtttgcc attgaagaca aagcgtccaa ggcctgagcg cgggaaatgc
     8941 ggggaaccat gtccatcgcc agcgcgttga ctttcttggc gcgcaaggcc tcgaccaaag
     9001 cctcgttttg gcgcggccac aggaagctga cgatggtttg accttctttg agcagcggca
     9061 gctcgccttc ggacggcgcg ttgaccttat aaattaaagg gcaggcccaa accgccgctt
     9121 tgtcggcaac ggttgcgcct gctgtttggt aagcggcatc gtccaaactt gccgccaaac
     9181 ctgcaccgct ttcgacaacg gtttcaaagc ctagtttgcc cagcagggca acggtggcgg
     9241 gcgtgcaggc gacgcgggtt tcgccggata atgactcgcg tgggataccg attttcatct
     9301 ctgaatcctt tttcgggttg ttcatatgta tcgtgggtta aatttaaatc ggtgcagggc
     9361 ggagcaacgc cgtaccggtt taaaaccgat tcacttcaaa tgttaatata ttttagataa
     9421 tccccttata acgaattttc atcaggctgg caatagttgc gcattttccc gtgttgtccg
     9481 acacatactg ccgtttgcgt ttgaagtcgt tttccgattg caggcacgcc cgccgtaaca
     9541 ttctttgccg tttcactata taatccgcat tttttgagcc gccgttatgc cgcaagccct
     9601 cgtcctccaa tttccctccg ccgaagccct gccttccgac ttcccctcac gcctgcccga
     9661 acctgattat gctgatgaaa agcgtatgcg ttttattgtt gaagaaggat tttctttaag
     9721 cgaaaaagac gcggcgttac tggacagccg tcaaatcgac cacgccgttt tgcccaatat
     9781 ggcgtttggc gaactcgggc tgattgtcag cgatatggat tcgacgctga ttaccatcga
     9841 atgtatcgat gaaattgcgg caggtgtggg tttgaaaggc aaagtagcgg aaattaccga
     9901 gcgcgccatg cgcggagagt tggatttcgg acagtcttta cgcagccgcg ttgcgttgtt
     9961 ggcgggattg gacgaacaga ttttggcgga catttatgaa aacgttttga agctctcgcc
    10021 cggtgcggaa tttttgttgg acgaatgcaa aaggcacaat gtgaaattcc tgctggtgtc
    10081 cggcgggttc acgtttttta ccgaaaggct gcaacaacgg ctcggcttcg aataccaaca
    10141 cgccaatatt ttggaaattg aaaacggcag gttgaccggc cgtctgaaag gcagaatcat
    10201 cgacgcgcag gcaaaggcgg atttattgcg cgaataccgc agccgcctcg gattgcagcc
    10261 gcatcaggtg ttggcgatgg gcgacggtgc gaacgatatt ccgatactca aagaagcggg
    10321 cataggcgtg gcttaccgcg ccaaaccgaa agcgcgggcc gccgccgatg cctgtatcaa
    10381 cttcggcggt ttggagcgtg tacgcggcct gttcggatag gcggatagga aacggatgcc
    10441 gtctgaaagg ttttcagacg gcatttgaac ggcaggaacg acagtgggac gcagaaaact
    10501 ttggtttgcc ctggcggcag caggcgttat cggcggtttg gtcggcattg tgctgacgga
    10561 actgatgcac ttcatacggc atacggcata cggttatgac gcggacggtg cgtacatctc
    10621 gttccgcgaa ggtgtggcac aggctttcgg tatgcggcgc gttgccatgc tgacgctgtg
    10681 cggcgcgatc gcaggcggcg gctggtggct gctcaaacgt ttcggcaagc cgcaaatcga
    10741 aatcaaagcc gccttgaagc agccgttgca ggggctgccg tttctgacga cggttttcca
    10801 tgttctgctg caaatcataa cggtcggact cggttcgccg ctcggacgcg aagtcgcccc
    10861 gcgtgaaatg actgccgcgt ttgcttttgc cggcggcagg cggttgggtt tggacgaagg
    10921 cgaaatgcgc ctcttgattg cctgtgcgtc cggagcgggt ttggcggcgg tgtataacgt
    10981 gccgctcgcc tccacgctgt ttatcctcga agcgatgttg ggcgtgtgga cgcagcaggc
    11041 ggttgccgcc gcattgctga cttccgtcat cgccaccgcc gtcgcgcgca tcggcttagg
    11101 cgacgtgcag cagtatcatc ccgccaacct tgccgtaaat acttcattgc tttggttttc
    11161 cgccgtcatc ggcccgatac ttggcgcaac cgccgccttt ttccggcgca ccgcccaaaa
    11221 attccccttc atcaagcgcg acaatatcaa aatcattccc ttggccgtct gtatgtttgc
    11281 actcatcggt gtgattgccg tttggtttcc cgaaattttg ggcaacggca aagcaggcaa
    11341 ccaactgact tttggcggtt tgaccggctg gcaatacagc cttgagttga ccgctgtcaa
    11401 atgggcggtc gttttgatgg cgctggcggc aggcgtatac ggcggactga ttacgccgtc
    11461 tatgatgctc ggcagcacca tttcttttgc agccgccgcc gcgtggaaca gcgtcttccc
    11521 cgaaatgccg tctgaaagcg cggcagtcgt cggagcggcg gtgtttctcg gcgtatcgct
    11581 caaaatgccg ctgaccgcca ttgtgttcgt attggaactg acctacgcgc cgctgtccct
    11641 gctgctgcct ttgtgcatcg gcatggcggg ggcattggga acatcgggaa aaatgggctt
    11701 caaatgaatg ccgacaaatg ccgtctgaaa ggtttccaga cggcatttct ttgcaaattg
    11761 tcaacaatat ctgcaaaaac ctactgccaa aaaactgaaa atagcggata ataccgcccc
    11821 gaaaaagatc ccctttcacc atccaaccgt cagaaaagga agccccaatg tccgtcatca
    11881 aacgcgccct gatcagcctg tccgacaagg caggcgcggt tgaatttgcc caaaacctgc
    11941 acaagctcgg tgtcgaaatc ctctccaccg gcggcacggc gaaactgctt gctgatgcgg
    12001 gcgttcccgt gattgaagtc gccgactaca ccggttttcc cgaaatgctc gacggccgcg
    12061 tgaaaaccct gcatccgaaa atccacggcg gtattttggg ccggcgcgat ttggacgaac
    12121 acgtcgccaa gatggaagag cacggcatcg gcaacatcga ccttgtgtgt gtcaatcttt
    12181 accccttcgc cgccaccatc gccaagcccg gctgcacact ggaagacgca attgaaaaca
    12241 tcgacatcgg cggcccggcc atggtgcgtt ccgccgcgaa aaactggaaa cacgtcgcca
    12301 tcgttaccga taccgccgac ttccccgcca ttgccgccga actcgaagcc aacaacggcg
    12361 cgttgagcga caaaacccgt ttcaacctct cacgcaaagc attcagccac accgcccaat
    12421 acgacggcat gatttccaac tacctgacct ccctttcaga cgacgttttg agcggccagc
    12481 cccaaatcgg cgaattccca agccggttca accaaagctg gattaaagtg caagacatgc
    12541 gctacggcga aaacccgcat cagcgcgccg cgttttaccg cgatattgac cccgccgcag
    12601 gcagcctttc cgcctacaat caactgcaag gcaaagaatt gtcttacaac aacatcgccg
    12661 atgccgatgc cgcttgggaa gccgtcaaat cctttgacgc gcccgcctgc gtgatcgtga
    12721 aacacgccaa tccgtgcggc gtagccgttg cagccgatac cttgaccgcc tacaaactcg
    12781 cctacgccac cgacaccacc agcgcgttcg gcggcatcat cgccttcaac cgcgaagtcg
    12841 acggcgaaac cgtcaaacag attaccgaca accaatttat ggaagtcctc atggcgccga
    12901 agtttaccgc cgaagccctt gaaatcgccg ccgccaagaa aaacgtgcgc gtattggaag
    12961 tgccgctcaa agcaggtgcc aaccgctttg aactcaaacg cgtcggcggc ggactgttgg
    13021 tacaaacgcc cgacatcaac cgcatcaacc gcgccgattt gaaagtcgtc tccaaacgcc
    13081 aaccgaccga gcaggaatgg aacgatttgc tgtttgtctg gaacgtcgca aaatacgtca
    13141 aatccaacgc catcgtcttc ggcaaaggcg gccaaaccta cggtatcggc gcaggccaaa
    13201 tgagccgcgt ggacagcacc cgcatcgccg cccgcaaagc gcaagatgcc ggtctcgacc
    13261 tcaacggcgc atgtgccgct tccgatgcct tcttcccatt ccgcgacggc gtggacgtga
    13321 ttgccgaaca gggcatcaaa gccatcatcc atccggcagg ctcgatgcgc gatcaggaag
    13381 ttttcgacgc ggcggacgaa cacggcatcg ccatggcggt aaccggcatc cgccacttcc
    13441 gccattaagg cagacgaaca aggcaatgcc gtctgaaggg ttttcagacg gcatttttgc
    13501 gctatttacc gtaatcctga aatcccgtca ttcccacgca ggcaggaatc tagatgagaa
    13561 tccatcctgt cagaaatacc cgaatcgtca ggaatacccg tgtcatcatt cccgatacac
    13621 cgtaatcctg aaacccgtca ttcccgcgca gacagaaatc cggatttgtc cgcgcggaaa
    13681 cttatgcgcc gccattcccg cgaaggcggg aatccggacc gttcggtttc ggttatttcc
    13741 gataaattcc tgctgctttt cattcctaga ttcccacttt cgcgggaatg acgaaggagt
    13801 gggaatccgg tttttgagtt ccggccattt ccgacaaatt gctttggcat tggatatttc
    13861 ctattttaat ccactatagt ggattaactt taaaccagta cggcgttgcc
//
LOCUS       DAXDSD010000062        13776 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXDSD010000062 DAXDSD010000000
VERSION     DAXDSD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.062"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..1205)
                     /gene="dxs"
                     /locus_tag="ACFB2Q_001399"
     CDS             complement(<1..1205)
                     /gene="dxs"
                     /locus_tag="ACFB2Q_001399"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC0918410.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAATDKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVA"
     gene            complement(1290..2207)
                     /gene="xerC"
                     /locus_tag="ACFB2Q_001400"
     CDS             complement(1290..2207)
                     /gene="xerC"
                     /locus_tag="ACFB2Q_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC0918411.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            2401..3465
                     /gene="fba"
                     /locus_tag="ACFB2Q_001401"
     CDS             2401..3465
                     /gene="fba"
                     /locus_tag="ACFB2Q_001401"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC0918412.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            3604..4461
                     /locus_tag="ACFB2Q_001402"
     CDS             3604..4461
                     /locus_tag="ACFB2Q_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC0918413.1"
                     /translation="MTRSKPVNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGL
                     ADALTAPLDHKDKGLKSLTLEASIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKND
                     KISRFDFVQKIEVDGQTITLASGEFQIYKQDHSAVVALRIEKINNPDKIDSLINQRSF
                     LVSDLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPE
                     QNVELASAELKADEKSHAVILGDTRYGGEEKGTYRLALFGDRAQEIAGSATVKIGEKV
                     HEIGIADKQ"
     gene            4600..5277
                     /gene="tsaB"
                     /locus_tag="ACFB2Q_001403"
     CDS             4600..5277
                     /gene="tsaB"
                     /locus_tag="ACFB2Q_001403"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC0918414.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGGYPATGAAHAELLYVRNKIALTAKEQ
                     AERKART"
     gene            5274..5714
                     /gene="rimI"
                     /locus_tag="ACFB2Q_001404"
     CDS             5274..5714
                     /gene="rimI"
                     /locus_tag="ACFB2Q_001404"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC0918415.1"
                     /translation="MNIRPALPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCAADGQTEDAVLMEKIC"
     gene            5708..6445
                     /locus_tag="ACFB2Q_001405"
     CDS             5708..6445
                     /locus_tag="ACFB2Q_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC0918416.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            6507..7148
                     /gene="pyrE"
                     /locus_tag="ACFB2Q_001406"
     CDS             6507..7148
                     /gene="pyrE"
                     /locus_tag="ACFB2Q_001406"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC0918417.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            7222..7650
                     /locus_tag="ACFB2Q_001407"
     CDS             7222..7650
                     /locus_tag="ACFB2Q_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC0918418.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            7647..8957
                     /gene="argA"
                     /locus_tag="ACFB2Q_001408"
     CDS             7647..8957
                     /gene="argA"
                     /locus_tag="ACFB2Q_001408"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC0918419.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNPHILVRRLHR"
     gene            9053..11068
                     /locus_tag="ACFB2Q_001409"
     CDS             9053..11068
                     /locus_tag="ACFB2Q_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC0918420.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQNAEYPKGVYRMCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(11181..12137)
                     /locus_tag="ACFB2Q_001410"
     CDS             complement(11181..12137)
                     /locus_tag="ACFB2Q_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC0918421.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFGLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGRLRRLRLEHAL
                     ALIESGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            12373..13344
                     /locus_tag="ACFB2Q_001411"
     CDS             12373..13344
                     /locus_tag="ACFB2Q_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC0918422.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKRKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            13377..13700
                     /locus_tag="ACFB2Q_001412"
     CDS             13377..13700
                     /locus_tag="ACFB2Q_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC0918423.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
BASE COUNT         3031 a         4218 c         3827 g         2700 t
ORIGIN      
        1 atcgccacga cgggcttcat gccttcgcac gccaaaccgc cggcaaaggt aacggcgtgc
       61 tgctcggcga tgccgacatc gaaatagcgg tcggggaatc gttgttcaaa ctccaccagt
      121 ccgctgccct cgcgcatggc gggggtaatc gcaaccagtc gggaatctgc cgccgcccgg
      181 tcgcacagcc atttgccgaa tacttgggta taggtcggtt tggcggcggg cttgggttct
      241 ttttcagacg gcatttgcgc cccgccttct ttaggcaggt tggcgacggc gtggtatttg
      301 acggggtcgt tttcggcgag tttgtagccg ttgccctttt tggtgatgac gtgcagcaac
      361 tgagggcctt tgcggctgcg caagtctttc aatacgtcca ccagattctc gacgttgtgt
      421 ccgtccacgg ggccggtgta gcggaagccg aaattttcaa acagcgacag cgactgtttg
      481 gcgtgttcgg cttcttcggc aagggttttg attttgtgtt cgactttttg ggcaaactcc
      541 atcgcgccgg gtattttgtc taataccttg cccgtttgcg ctttgacggt actcaacagt
      601 ccgtgcatat cgcgcacgac gttgctggca agatatttgg gcaacgcgcc gacgttgggg
      661 gaaatcgaca tttcgttgtc gttgaggacg accagcaaat ccacatccat atcgcccgcg
      721 caattcaagg cttcaaacgc ctgccccgcc gtcatcgcgc cgtctccgat gatggcgacg
      781 ctgcggcggt cgccgcccaa gagtttgtcc gtcgccgcca tgcccaaagc cgcgccgatg
      841 gaggtggagg aatgccccac gccgaacgcg tcgtactcgg actcgcaacg tttcggaaaa
      901 cccgccaaac cgccgtattg gcgcatggtg tgcatctggt ttttcctgcc tgtcaggatt
      961 ttgtgcgggt agctttggtg tccgacatcc cacaccagct tgtcttcggg cgtgtcgtac
     1021 acatagtgca gggcgatggt cagttcgacc gcacccagat tgctggcgaa atgcccgccg
     1081 gtctgcccga cagattccag cagaaaggcg cgcaactcgc cggcaaggcg cggcagctgt
     1141 tttttgtcca ggcggcgcaa atcttgcggg ctgtcaatca ggtcgagtag ggggcttggg
     1201 ttcatggtgt gtctttttta tgtgtcgtcc gggtgcaacg gtcaattata tatcaagagc
     1261 gtgcggctga cggctgattt tgccgtacgt cattcgtcct gccgcttggc gcgcgggtgg
     1321 gcttcgtcat agaggcgggc gatgtggtcg aaatcgagct tggtataaat ctgcgtggtc
     1381 gaaaggctgc tgtgtccgag tagctcctgc accgccctga tgtcgcgcga agcctgcaac
     1441 aggtggccgg cgtagctgtg gcgcatcata tgcggcgaaa cgtgcctgcc gtcgccgtat
     1501 tgcgccgccc acgattcgag gcgtttttgg atttggcgtt ggctcaggcg cgtgccgttc
     1561 ctgccggtaa acagggcttt gccgtccgat gccgtctgac gcagcggcag atagtttttc
     1621 aaggcttcca cgcttttgcc ggtcagcgga acctgacgct gcttgcgccc tttgccggta
     1681 acgtgtaccc acgcttcgtc caaatataca tcatctgcat tcaagccgtg tatctcgctc
     1741 acgcgcaaac cgctgccgta catcagctcg aacagcgcgt ggtcgcgcac cgccagcggg
     1801 tcgccgccgt ccacgggcaa atccaacatc cggttcagcc attcctgcgg cagggctttg
     1861 ggtacgcgct cgggctgctt cggcggtttg atgtcggcgg tcgggtcggc gcgcatcagc
     1921 ccgcgtttga ccagccaggc gcaatactgc cgccacgccg acagcttgcg cgccagcgtc
     1981 cgaccgtcca aaccgcgctg cgacagccgc cgcaacgccg ccgtaaaatc gcgccgcgac
     2041 aagtcctgcg gcacgccgcc tgcatcttca gacggcattt gtgccaacag tgcaaacagt
     2101 tcttccaaat cgcgccggta tgccgcaacc gtgtgctccg acttgccctc gcgcacgatg
     2161 ttttccaaat aagcgtcaaa atacgccgca aacccgtcca aaaccataac cgtcccacac
     2221 aaatatcaaa aaaccagtga aaacattatc ataaacaaca caccaaaatc ccgaaccgcc
     2281 caaaatccgc cccatctgcc cgccgttatc gttaatataa aatcaatgag ccgtttatgg
     2341 ttttttgccg taaaaaacat tataatccgc cttatttacc tattgcccaa ggagacacaa
     2401 atggcactcg tatccatgcg ccaactgctt gaccacgccg ccgaaaacag ctacggcctg
     2461 cccgcgttca acgtcaacaa cctcgaacaa atgcgcgcca ttatggaagc cgccgaccaa
     2521 gtcaacgcgc ccgtcatcgt acaggcgagc gcaggtgcgc gcaaatacgc gggcgcgccg
     2581 tttttgcgcc acctgattct ggcggcagtc gaagaatttc cgcacatccc cgtcgtgatg
     2641 caccaagacc acggcgcatc gcccgacgtg tgccaacgct ccatccaact gggcttctcc
     2701 tccgtgatga tggacggctc tttgctcgaa gacggcaaaa ccccttcttc ttacgaatac
     2761 aacgtcaacg ccacccgtac cgtcgtcaac ttctcccacg cctgcggcgt gtccgtcgaa
     2821 ggcgaaatcg gcgtattggg caacctcgaa accggcgaag caggcgaaga agacggagtg
     2881 ggcgcggcag gcaaactctc acacgaccaa atgctcacca gcgttgaaga tgccgtgcgt
     2941 ttcgttaaag ataccggcgt tgacgcattg gcgattgccg tcggcaccag ccacggcgca
     3001 tacaaattca cccgtccgcc cacaggcgac gtattgcgta tcgaccgcat caaggaaatc
     3061 caccaagccc tgcccaatac acacatcgtg atgcacggct ccagctccgt tccgcaagaa
     3121 tggctgaaag tcatcaacga atacggcggc aatatcggcg aaacctacgg cgtgccggtt
     3181 gaagaaatcg tcgaaggcat caaacacggc gtgcgcaaag tcaacatcga taccgacctg
     3241 cgcctcgctt ccaccggcgc ggtacgccgc taccttgccg aaaacccgtc cgactttgat
     3301 ccgcgcaaat acttgggcaa aaccattgaa gcgatgaagc aaatctgcct cgaccgttat
     3361 cttgcgttcg gttgcgaagg tcaggcaggc aaaatcaaac ctgtttcgtt ggaaaaaatg
     3421 gcaagccgtt atgccaaggg cgaattgaac caaatcgtca aataacaggt tgcctgtaaa
     3481 caaaatgccg tctgaacgcc gttcggacgg catttgattt ttgcttctct gacctgcctc
     3541 attgatgcag tatgcaaaaa aagataccat aaccaaaacg tttatatatt atctattctg
     3601 tgtatgacta ggagtaaacc tgtgaaccga actaccttct gctgcctttc tttgaccgcc
     3661 ggccctgatt ctgaccgcct gcagcagcgg aggggcggag gcggtggtgt cgccgccgac
     3721 atcggcacgg ggcttgccga tgcattaacc gcgccgctcg accataaaga caaaggtttg
     3781 aaatccctaa cattggaagc ctccattccc caaaacggaa cactgaccct gtcggcacaa
     3841 ggtgcggaaa aaactttcaa agccggcggc aaagacaaca gcctcaacac gggcaaactg
     3901 aagaacgaca aaatcagccg cttcgacttc gtgcaaaaaa tcgaagtgga cggacaaacc
     3961 atcacactgg caagcggcga atttcaaata tacaaacagg atcactccgc cgtcgttgcc
     4021 ctacggattg aaaaaatcaa caaccccgac aaaatcgaca gcctgataaa ccaacgctcc
     4081 ttccttgtca gcgatttggg cggagaacat accgccttca accaactgcc tgacggcaaa
     4141 gccgagtatc acggcaaagc attcagctcc gacgatgccg gcggaaaact gacctatacc
     4201 atagatttcg ccgccaaaca gggacacggc aaaatcgaac acctgaaaac acccgagcag
     4261 aatgttgagc ttgcctccgc cgaactcaaa gcagatgaaa aatcacacgc cgtcattttg
     4321 ggcgacacgc gctacggcgg cgaagagaaa ggcacttacc gcctcgccct tttcggcgac
     4381 cgcgcccaag aaatcgctgg ctcggcaacc gtgaagatag gggaaaaggt tcacgaaatc
     4441 ggcatcgccg acaaacagta gtatttgaaa aatgccgtcc gaacacgata atttaccgtt
     4501 cggacggcat ttttgcgcca taccgcccta ggacacgacg gcacggcagg caagccgatt
     4561 tcactatata atgccgtctg aaccaacgag aaccacacca tgcaagccga tttcaaccgc
     4621 cccgtcctcg ccgtcgatac cggaacttcc tatttgtcgc tcgcgctgcg cgccgacggc
     4681 gaaatccgtc tgttccatca ggaagtcggc atccgccagt ccgaactgat cctgccggaa
     4741 atccgcaccc tattccgaaa tgcgggcatt accgccgccg atttgggggc gattgtttat
     4801 gcgaaaggcc ccggcgcgtt taccggactg cgtatcggca tcggcgtggc gcaaggtttg
     4861 gcaacgccgt tcgacacgcc tttaatcggc atccccacgc tcgatgccgc cgcctcgctg
     4921 ccgccgccgc aaagctgcat actcgccgct gcggacgcgc gtatgggcga agtgttttat
     4981 gcgtggttcg acacgctgaa ccgccgccgt ttgagcgatt atcaagtcgg tagggcggca
     5041 gacattgccc tccccgaggg gtacgtcttt tcagacggca tcggcagtgc gttcgcactg
     5101 gaaaaccgtc cgcccttctc aggcaaaccc gatatgccga ccgccgccga ttttctcgcg
     5161 ctcgccctcg gcggcggcta tcccgcaact ggcgcggcac acgccgaact gctctacgtc
     5221 cgcaacaaaa tcgccctgac tgccaaagaa caggcggaac ggaaggcacg aacatgaaca
     5281 tccgcccagc cttaccgtcc gactgcgccg cactggccgc gctcgatacc gtctgcaacc
     5341 cgtccgcgtg gacgcagcgc caatttgagt ccgcgcttgt ttcgccgtcc gaacaggttt
     5401 tccttgcgga aaaagacggc aggattgccg cctttatcgt ttggcagaac ctgcccgacg
     5461 aatccgaact gcacctgatt gccaccgcgc ccgaatgccg ccgacggggc gttgcgtccg
     5521 ccctgctcga atattggtac gcacatctgc ccgaaggcac gcaacgcctg ctgctcgaag
     5581 tccgcgcagg caacgccgcc gcacaggcac tgtataccaa acacggcttc agcattgcgg
     5641 gcaggcggaa aaactattac tgcgccgccg acgggcaaac cgaagatgcc gtcttaatgg
     5701 agaaaatatg ttaagcgcgc gctacctcca cctgcacgag gctttgggct tgggtccgat
     5761 gtggctgaaa caggccgccg ccgtcctgcc gcccaaaaac acacccgcaa ccccggcaca
     5821 ggcacgtccc caaaccgtcc gcgccgccac gatccgccct tcccaacccc ataacgttca
     5881 ggcgcggctc gaaacgatga aagcgttgga aaccgccgcc gtacatacgc gcaaacccgc
     5941 gcctgaaacc gaaacgcccc cgcccggcct ttcagacggc atcgcccccg ttcccgccgc
     6001 ttcgggcatc accaagcttg ccgtcgtcag cctgtgtccg ccgatcgagg atgcggttta
     6061 cgggcaactg ttccacggca aagcgggcat cctgctcgac aacatcctca aagccgcagg
     6121 actggatgcc gcctatgtcc acaaaacctg ttgggtgaaa accgccgccg tcggtaaccc
     6181 gatgccgtct gaacaggccg tcgcggatgc gctgggtcaa atcgccgggg aactcgacgg
     6241 ctgccgcgcc cccgccgtcc tattcctcgg acaggcgttt gtcaacctgg aacggcaggc
     6301 gatgattgaa actttgtgcg gcagccgtcc cttcttcatc atcgaccatc ccgcccggct
     6361 gttgcgccaa cccgaactca aagcccgaaa ctggcaggtg ttgaaacagt tgaaacgcgc
     6421 cttgcggcaa ggcggcggca gttgaagcgc gccgcacggg gcggtagaat cgcaactgcg
     6481 tcccaatatc tgacagaaag cacaaaatga ctgatttccg ccaagatttc ctcaaattct
     6541 ccctcgccca aaatgttttg aaattcggcg aatttaccac caaagccgga cggcggtcgc
     6601 cctatttctt caatgccggc ctcttcaacg acggcgcgtc cacgctgcaa ctggcaaaat
     6661 tctatgcaca atccatcatt gaaagcggca tccgattcga tatgctgttc ggccccgcct
     6721 acaaaggcat tattttggcg gcggcaaccg cgatgatgct ggcggaaaaa ggcgtgaacg
     6781 tcccgtttgc ctacaaccgc aaagaagcca aagaccgcgg cgaaggcggc gtgttggtcg
     6841 gcgcgccgct taaagggcgc gtgctgatta tcgacgacgt gatttccgcc ggcacatccg
     6901 tacgcgaatc aatcaaactg attgaagcgg agggtgcaac ccccgccggt gtcgccatcg
     6961 cgctcgaccg catggaaaaa ggcacgggtg aattgagcgc ggttcaggaa gtggaaaaac
     7021 aatacggcct gcccgtcgcc cccatcgcca gcctgaacga tttgtttatc ctgttgcaaa
     7081 acaaccccga attcggacag ttcctcgaac ccgtccgcac ctaccgccgg cagtacggcg
     7141 tagaataaaa ataaagcata tgccgtccga accacctgcc gcttcagacg gcatcaaacc
     7201 gacacacacc gagaaaacat catgcccgcc tgtttctgtc cgcactgcaa aacccgcctc
     7261 tgggtcaaag aaacccagct caacgtcgcc caaggcttcg tcgtctgcca aaaatgcgaa
     7321 gggctgttta aagccaaaga ccatctggca agcacgaaag aacctatatt caacgatttg
     7381 cccgaagctg tttcggatgt caaactcgtc caccgcatcg gcacgcacgc cattagcaag
     7441 aaacagattt cccgcgacga aatcgccgat atcctcaacg gcggtacaac cctgcacgat
     7501 acgccgcccg caaccgccgc tgccgcacct gccgccgcac cgcaggtttc cgtaccgccc
     7561 gcccgtcagg aagggctcaa ctggactatt gcaaccctgt tcgcacttat cgtcctcatt
     7621 atgcagcttt cctacctctt catcctatga acgcgcccga cagctttgtc gcccacttcc
     7681 gcgaagccgc cccctacatc cgccaaatgc gcggcacgac actggtcgcc ggcatagacg
     7741 gccgcctgct cgaaggcggc accttaaata agctcgccgc cgacatcggg ctgttgtcgc
     7801 aactgggcat ccgactcgtc ctcatccacg gcgcgtacca cttcctcgac cgcctcgccg
     7861 ccgcgcaagg ccgcacgccg cattattgcc ggggtttgcg cgttaccgac gaaacctcgc
     7921 tcggacaggc gcagcagttt gccggcaccg tccgcagccg ttttgaagcc gcattgtgcg
     7981 gcagtgtttc aggattcgcg cgcgcgcctt ccgtcccgct cgtatcgggc aacttcctga
     8041 ccgcccgtcc gataggcgtg attgacggaa ccgatatgga atacgcgggc gttatccgca
     8101 aaaccgacac cgccgccctc cgtttccaac tcgacgcggg caatatcgtc tggatgccgc
     8161 cgctcgggca ttcctacggc ggcaaaacct tcaatctcga tatggtgcag gccgccgctt
     8221 ccgtcgccgt ctcgcttcag gccgaaaaac tcgtttacct gaccctttca gacggcattt
     8281 cccgccccga cggcacgctc gccgaaaccc tctcggcaca ggaagcgcaa tcgctggcgg
     8341 aacacgccgc cagcgaaacc cgacgactga tttcgtccgc cgttgccgcg ctcgaaggcg
     8401 gcgtgcatcg cgtccaaatc ctcaacgggg ccgccgacgg cagcctgctg caagaactct
     8461 tcacccgcaa cggcatcggc acgtccattg ccaaagaagc cttcgtctcc atccggcagg
     8521 cgcacagcgg cgacatcccg cacatcgccg ccctcatccg cccgctggaa gaacagggca
     8581 tcctattgca ccgcagccgc gaatacctcg aaaaccacat ttccgaattt tccatcctcg
     8641 aacacgacgg caacctgtac ggctgtgccg cactcaaaac ctttgccgaa gccgattgcg
     8701 gcgaaatcgc ctgccttgcc gtctcgccgc aggcacagga cggcggttac ggcgaacgcc
     8761 tgcttgccca cattatcgat aaggcgcgcg gcataggcat aagcaggctg ttcgcactgt
     8821 ccacaaatac cggcgaatgg tttgccgaac gcggctttca gacggcatcg gaagacgagc
     8881 tgcccgaaac gcggcgcaaa gactaccgca gcaacggacg aaacccgcat attctggtgc
     8941 gtcgcctgca ccgctgaccg caacggaaag ccgccgcaga aatgccgcct gaaccccgtt
     9001 tcagacggca tttccccgat tataaagacc tgcccgaccc tcaaggaccc cgatgaaatc
     9061 ctaccccgac ccctaccgcc attttgaaaa cctcgattcc gccgaaacgc aaaacttcgc
     9121 tgctgaagcg aatgccgaaa cgcgcgcgcg ttttttaaac aacgacaagg cgcgcgcact
     9181 ttcagacggc attttgaatc aaatgcagga cacgcggcag attccgtttt gtcaggaaca
     9241 ccgcgcgcgg atgtaccatt tccatcagaa tgcggaatat ccgaagggcg tgtaccgcat
     9301 gtgtacggcg gcgacctacc gttccggcta tcccgagtgg aaaatcctgt tttcggtggc
     9361 ggatttcgat gagttgctcg gcgacgatgt gtatttgggc ggcgtgtcgc acttggtgga
     9421 gcagcccaac cgcgcgctgc tgactttgaa caaatcgggc ggcgatacgg cgtatacgct
     9481 ggaagtggat ttggaagcag gggaattggt agagggcggt tttcactttc cggcaggcaa
     9541 aaaccatgtg tcgtggcgcg atgaaaacag cgtgtgggtg tgtccggctt gggacgaacg
     9601 ccagttgacc gaatcgggct atccgcgcga agtgtggctg gtggaacgcg gcaagagttt
     9661 cgaggaaagc ctgccggcgt accaaatcga taaaggcgcg atgatggtaa acgcgtggcg
     9721 ttacctcgat ccgcagggtt cgccgattga tttgattgaa gcgtcggacg gtttttacac
     9781 caagacgtat ttgcaggtgt cgtccgaagg cggggcgaaa ccgttgaacc tgcctaatga
     9841 ttgcgatgtg gtcggctatc tggcgggaca tcttttgctg acgctgcgca aggactggca
     9901 ccgcgcgaac caaagctatc cgagtggcgc gttggtggcg gtgaaactga atcggggcga
     9961 actcggggcg gcgcagcttt tgtttgcgcc cgatgaaacg caggcattgg aaagcgtgga
    10021 aacgaccaag cgttttgtgg tggcaagcct gctggagaat gtacaaggcc gtctgaaagc
    10081 gtggcggttt gccgacagca aatggcagga agccgagttg ccgcacctgc cctccggcgc
    10141 gttggaaatg accgaccaac cgtggggcgg cgacgtggtt tatcttgccg ccagcgattt
    10201 caccacgccg ctgacgctgt ttgcgctgga tttgaacgtg atggaactga ccgtcatgcg
    10261 cctccagccg cagcagtttg tttcagacgg catcgaagtg cggcagtttt gggcggtgtc
    10321 gtccgacggc gaacgcattc cttatttcca cgtcggcaaa aacgccgcgc ccgacacgcc
    10381 gaccttagtc tatgcttacg gaggtttcgg cattcctgaa ttgccgcatt atctgggcag
    10441 cgtcggcaaa tattggctgg aagagggcaa tgcctttgta ttggcaaaca tccgcggcgg
    10501 cggagaattc ggcccgcgct ggcatcaggc ggcgcaggga atcagcaaac acaaaagcgt
    10561 tgatgatttg ttggcagtcg tgcgtgattt gtccgaacgc ggcatgagtt cgcccaaaca
    10621 catcggcttg cagggcggca gcaacggcgg cctgattacc gccgccgcct tcgtgcgcga
    10681 accgcaaagc atcggtgcgc tggtgtgcga agtaccgctg accgatatga tccgttatcc
    10741 gctgctgtcc gccggttcaa gttggacgga cgaatacggc aatccgcaga aatacgaagc
    10801 ctgcaaacgc cggctgggcg aattgtcgcc gtatcacaat ctttcagacg gcatcgatta
    10861 tccgcccgca ctcattacca ccagcctcag cgacgaccgc gtccatcccg cccacgcgct
    10921 caaattctac gccaaactgc gcgaaacctc gccgcaatct tggctctact cgcctgacgg
    10981 cggcggccat accggcaacg gcacccaacg cgaatccgcc gacaaactcg cctgcgtgtt
    11041 gctgtttttg aaagaatttt tgggataagg gcagggggcg acactaccgc cgcgaatgaa
    11101 aaaaggtcgt ctgaaattgc tttttcagac gacctttttt aatggttgtt tcaaatcaaa
    11161 atatctatgc cgtcggccca tcagcacttt ttcacatccg aaggcaaaaa tccgtaatgc
    11221 cgcctgaacg cctcgttgaa ccgtcccgcg tggcggtagc cgcaaaagtg catcgcggct
    11281 tggatggtgc tgcccgattc gatgagggcg agcgcgtgtt ccagccgcag gcggcgcagg
    11341 cgtccggcga cggtttcgcc ggtttgcgct ttgaaatagc gtttcaggta gcattcgttc
    11401 agcccgacgc ggcgggcgat ttcggcgatg gtcagcgggc gggcgaattc gctgttcaaa
    11461 atatcggcgg cttcgtctat gcgccggcgg cggtagccgt tgtcgtggcg gcggaaggtg
    11521 aagcgtaata atcgggcgga gagttccagc gcgtcggctt cgtcggcgag caggccgaag
    11581 ccgtccgatt cgaacgggcg ttgcagcagg gggcaggccg ccgccgtcag tgtccccgcg
    11641 ttttgcgcca gccgttgcag ggcgaatcgg cctatcgttt gcggcgaaaa caggcgttcg
    11701 tccagtaaac cttcgtcgtg ccagcggcgc agtttttcca gcgaaaaatc caaatgcagc
    11761 gcgcacatgc cgccgctgtc tggcagcagg gtttcggata cgtccgccag ttcgccgcgt
    11821 accagccaga tttcgccggc agacgggcgg tattccctgc cgcccatttg taaccggttc
    11881 tgccccgata ccatgacgaa caaggcgcag ttgtggctga aattgtggat ttcggtggga
    11941 aacgcacccg ttccgccgcc gcgcatccgt gataaggtga tgcccgagtc gaagcggttg
    12001 atgcacattt tcagatgcaa accgggctgt tttgcctgcg caatgagtgc gctgtcggaa
    12061 cagccgtcca acgcccagcc ggatttatcg gagcggacat aggtttggta ctggcggtag
    12121 atggcggcgg tgttcatgat tggataggaa cgagttgtct aacaaatgaa ttaaatagga
    12181 attattacca ataatcaagc acagggattg gttggaacgg aaaaggtcgt ctgaaagggt
    12241 gtttcagaca accttttccg tttcgggaat ttgttttgcc gtatcgggaa ttttgcgttt
    12301 tgcggcgtgg tttctgcagg ttgtttgctt aataataaac attcttattc gtatgcaaag
    12361 gaaccgcaca ccgtgaaacc gcgtttttat tgggcagcct gcgccgtcct gccggccgcc
    12421 tgttcgcccg aacctgccgc cgaaaaaact gtatccgccg catcccaagc cgcatccaca
    12481 cctgtcgcca cgctgaccgt gccgaccgcg cggggcgatg ccgttgtgcc gaagaatccc
    12541 gaacgcgtcg ccgtgtacga ctgggcggcg ttggatacgc tgaccgagcc gggcgtgaat
    12601 gtgggcgcaa ccaccgcgcc ggtgcgcgtg gactatttgc agcctgcatt tgacaaggcg
    12661 gcaacggtgg ggacgctgtt tgagcccgat tgcgaatccc tgcaccgcca caatccgcag
    12721 tttgtcatta ccggcgggcc gggtgcggaa gcgtatgaac agttggcgaa aaacgcgacc
    12781 accatagatt tgacggtgga caacggcaat atccgcacca gcggcgagaa gcagatggag
    12841 accctgtcgc ggattttcgg taaggaagcg cgcgtggcgg aattgaatgc gcagattgac
    12901 gcgctgttcg cccaaaagcg cgaagccgcc aaaggcaaag gacgcgggct ggtgctgtcg
    12961 gttacaggca acaaggtgtc cgccttcggc acgcaatcgc ggttggcaag ttggatacac
    13021 ggcgacatcg gcctgccgcc cgtggacgaa tctttacgca acgaagggca cgggcagccc
    13081 gtttccttcg aatacatcaa agagaaaaac cccggctgga ttttcatcat cgaccgcacc
    13141 gccgccatcg ggcaggaagg gccggctgcc gtggaagtgt tggataacgc gctggtatgc
    13201 ggcacgaacg cttggaagcg caagcaaatc atcgtcatgc ctgccgcgaa ctacattgtc
    13261 gcgggcggcg cgcggcagtt gatacaggcg gcggaacagt tgaaggcggc gtttgaaaag
    13321 gcagaacccg ttgcggcgca gtagttttat tcaaaacaaa ccacaaggag aaccccatgc
    13381 aaaacgaaac catcaacctg aaacagcacc ttgccgccat caaagaatac tggcagcccg
    13441 aaatcatcaa ccgccacggg ttccaattcc gcctggtcaa acttttgggc gattacggct
    13501 ggcacacgca cggatacagc gacaaagtgc tgtttgccgt ggagggcgac atggcggtgg
    13561 acttcgccga cggcggcaac atgacgatac gcgagggcga gatggcggtc gtgccgaagt
    13621 cggtgtcgca ccgcccgcgt tcggaaaacg gctgctcggt ggtgctgatt gatctgcccg
    13681 acccgtccga ggccgcctga aaacgaagtt tccgaaggaa gctgagtttc tgcgaagcta
    13741 aaagcagcct acaccttcaa tatgccaaaa gcatag
//
LOCUS       DAXDSD010000063        13740 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXDSD010000063 DAXDSD010000000
VERSION     DAXDSD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13740
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.063"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            1..75
                     /locus_tag="ACFB2Q_001413"
     tRNA            1..75
                     /locus_tag="ACFB2Q_001413"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34..36,aa:Glu,seq:ttc)
     gene            238..354
                     /locus_tag="ACFB2Q_001414"
                     /pseudo
     CDS             238..354
                     /locus_tag="ACFB2Q_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            512..1687
                     /locus_tag="ACFB2Q_001415"
     CDS             512..1687
                     /locus_tag="ACFB2Q_001415"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC0918424.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(2029..2577)
                     /locus_tag="ACFB2Q_001416"
     CDS             complement(2029..2577)
                     /locus_tag="ACFB2Q_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC0918425.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(2580..4076)
                     /gene="ccoG"
                     /locus_tag="ACFB2Q_001417"
     CDS             complement(2580..4076)
                     /gene="ccoG"
                     /locus_tag="ACFB2Q_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC0918426.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
     gene            4611..6590
                     /gene="tkt"
                     /locus_tag="ACFB2Q_001418"
     CDS             4611..6590
                     /gene="tkt"
                     /locus_tag="ACFB2Q_001418"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC0918427.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            6734..8122
                     /gene="fumC"
                     /locus_tag="ACFB2Q_001419"
     CDS             6734..8122
                     /gene="fumC"
                     /locus_tag="ACFB2Q_001419"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC0918428.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNRAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(8187..9110)
                     /locus_tag="ACFB2Q_001420"
     CDS             complement(8187..9110)
                     /locus_tag="ACFB2Q_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC0918429.1"
                     /translation="MENQRPLLGFALALLAAMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLTLGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFVYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRPFKRR"
     gene            complement(9483..10007)
                     /gene="ssb"
                     /locus_tag="ACFB2Q_001421"
     CDS             complement(9483..10007)
                     /gene="ssb"
                     /locus_tag="ACFB2Q_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC0918430.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQNQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(10011..11393)
                     /locus_tag="ACFB2Q_001422"
     CDS             complement(10011..11393)
                     /locus_tag="ACFB2Q_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC0918431.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(11505..12128)
                     /locus_tag="ACFB2Q_001423"
     CDS             complement(11505..12128)
                     /locus_tag="ACFB2Q_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC0918432.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPDEGERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            complement(12630..12896)
                     /locus_tag="ACFB2Q_001424"
                     /pseudo
     CDS             complement(12630..12896)
                     /locus_tag="ACFB2Q_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12933..13423)
                     /locus_tag="ACFB2Q_001425"
                     /pseudo
     CDS             complement(12933..13423)
                     /locus_tag="ACFB2Q_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3372 a         3942 c         3437 g         2989 t
ORIGIN      
        1 gcgcccatcg tctagcggtt aggacattgc cctttcacgg cggtaaccgg ggttcgattc
       61 cccgtgggcg tgccagattc caaatccccg aaataatccc tcggggattt ttattgcctc
      121 ggcaattcgt taccatatct ttacctaccc acttcatcag aatctcagac gtaatcgaat
      181 catattcaaa cttttgccat gcaaaccgat atcccataac cggatgcggt gtccgttcaa
      241 tatagtggat taaatttaaa tcaggacaat gcgacgaagc cccagacagt acaaatagta
      301 cggcaaggcg aggcaacgcc gtaccggttg aaatttaatc cactatattt tgtccgattg
      361 gtacagatat aacagcatat tccaaagctc cttctgcttt tctgtttacc actgcaatat
      421 ttagagtata ataccaaatt tgagtaatgg ttctaaaaca attaaaacca tttttcatga
      481 gcctgactga ttcgtatatt cggagaaact gatgcagaat atttttgacc ctttgattat
      541 tcgtggaaaa tcccttattc ccatcgtgca aggcggtatg ggggtcggtg tttccgcatc
      601 gggtttatcc agcgcggtgg cgcgtgaaaa cggcatcgga acgattgcca gtgtagattt
      661 gcgccacctt cacgaagacc tgctcgccga atcccaaatc aatccgagtg aagagaaata
      721 tacgtctttg aactgtaccg cattagacag ggaaatccaa aaagccaaaa gtgcttcaga
      781 gggaaaagga ctgattgcgg tcaacgtgat gaaggcggtc aaagaccacg ccgcgtatgt
      841 ccgccaggct tgcgaatcag gggcggatgc ggtcgtgatg ggtgccggcc tgcctttaga
      901 cctgccggaa atgaccgagg gctatcataa ggatgtcgcg ctgctgccga ttctgtccga
      961 atcgcgcggt attaatatcg tcttgaaacg ttggatgaaa aaaggcatat tgcccgatgc
     1021 gattgtagtc gaacatcctg cccatgcggc cggacatttg ggtgcatcaa ccgttgaagg
     1081 cgtaaacgat gccaaattcg acttcaaacg cgtgattgag gaaacgtttg aagtgtttaa
     1141 aaatttgggg ctggagggag aaaaaatccc gcttattctt gcgggaggca tggcaaattt
     1201 tgaaaaagtc aaaaccgccc taaagaactg gggagcctcc gccgttcaaa tcggtacggc
     1261 ttttgccgtt accgaagaag gagatgcaca cctcaacttt aaaaaaacgc tcgccagtgc
     1321 ggaaactgaa aaagtagtcg aatttatgtc tgttgccggt ttgccggcgc gcggtatccg
     1381 caccaaattc ctagacagct atatcaggcg cgaaggcaaa cttcaggcaa acgccaaagc
     1441 cgacccgcgc cgctgtaccc aaggtttaaa ctgcctgacc agctgcggtc tgcgcgacgg
     1501 gctttccaaa gcagggcaat tctgtattga tatccagctt gctgccgcat tccgtggaga
     1561 agttgataag gggttgtttt tcaggggaaa agaccagctt cccttcggta atgccatccg
     1621 caccgtccgc gaaacgatac aatatctgct gacggggagc gaacctgttg caacgctcgg
     1681 acgctgacat caggtaggat ttgatttgca aacaaaatgc cgtccgaagg gctttcagac
     1741 ggcgtttttc aggttgtgtt ccggaacagt gtcgaaaaaa cggagagaaa taaaaacgtt
     1801 tttatgtgaa caaaatgcct tccgcattat gtcggacata caacacaaat gccgtctgaa
     1861 gcctttcaga cggcattttt caggctgcct ttcggaatga tgttaaaaaa taaacgggat
     1921 gagatacatt tattttcgtc cgacaaatca gaccatcacg ccgaataatc aaacaatgcc
     1981 tgcccgacat tgcatataac aaaccaatgc aatgaaaaca cggctttttt atttgcttcc
     2041 tgcattattg aaaagtttgt ccatcggggt caaatcgacc gcattgccct ggctggtaat
     2101 ccatttgttc tcgacgcgcc acacgcctgc cgcgtcctcc acgcgcacat accagtggtt
     2161 ggccggcgga agggttttga acaccgcctc atattccgcc ctgccgttct gcgcgctgcc
     2221 gacgggcttg agggcgacgg tttgatcgtc cgccttgcgg gtcgggtgca tcagcagcag
     2281 gttcaaaggc tgtttgccgt caaactcgcc gccgacaaac acttttgccg cattcatatc
     2341 gggagaaatg aggacctgca ccccgatatg ccgtctgacg gcttcttcat cccgatgaag
     2401 ctggatgtcg atatgcttgc catccttata ataatcgtcc gtaaccaaat ctgtcgcgtg
     2461 ctgctgcgcg acaaaaaaca tagcgacgct ggcgatgacg acaaaaatcg gccccgccat
     2521 caagacccac ggccagacgt gtttgtacca aggcttgatt ggattgtttt gagacacggt
     2581 tattctccga taaaggttgc gtcttcttcc aaaacaaccg gcttgccgtc tgatgcaccg
     2641 ctttcccggt attggaagat aaactcgata ggatgactgc ctttgtccgc gtattccgga
     2701 atggtggaca cttggacggg aagggttatt gtttcgcgcg gagccacttt tataccgcct
     2761 tcaggcagcc cggtcagggc gatttcgtca aatcctttga cacttgcggt aatcagctgt
     2821 tctttttcgc tgttgttgat gatacgcagg ctatatgcgt tttccagcca acctttggcg
     2881 ttttcgcgca ccattacgcc acggtctttc agaatatcga cttcgaccat tttgcgcgtg
     2941 gacaaaccga ccaggaaggc ggcgacaacc actgccaaca ccgcgccgta acctgccacg
     3001 cgcggtctga gcagccgttt tttaatgtct ttttcggcat attcgtgttc cagcgcgctt
     3061 tcggtcgtat agcgtatcaa accgctcgga taacccattt tgtccataat ctcatcgcac
     3121 gcgtcgatac aggcggcgca gccgatacat tggtattgca ggccgttgcg gatgtcgatg
     3181 ccgacggggc agacttggac gcacatcgca cagttgatgc agtcgcccaa acccgcctct
     3241 tccttgttga ccgttttctt gcgggcgccg cgcggttcgc cgcgctccgc gtcataagaa
     3301 acaatcagcg tgtccttatc gaacatcgcg ctttggaaac gtgcgtacgg acacatatgc
     3361 aggcacactt tttcgcgcat aatatgggcg aagaagaagg tcataaagcc gtaaaacgct
     3421 gcggcaaaca tcgcgccgcc gcctgctgct ccggtgaata aatcgggaac aaactggcgg
     3481 atggggacaa accagccggc aaacgtgatg cccgtccacg cgcagacaag gaaaatcaac
     3541 aggtatttgg tggctttgat gcggattttg gtgaaattcc acggcgattt ttccagcttc
     3601 aggcgtttgt ttctatcgcc ttcgaccagg ttgtcgatcc acagcataat ttcggtgtaa
     3661 accgtttgcg ggcaggaata gccgcaccac aaacggccgg caatcgtcgt ccaccaaaac
     3721 agcccgaagg cgcaaatcat cagcagcagg gcaaggtaaa tcaaatcgcc cacccccaac
     3781 gacaatccga aaatgaagaa atgccgttcg gggatattga aaacgacggc ctgccttccg
     3841 ctccaattga accacggaat gacgtaaaac acaaactggg tcgccaatac ggcggcgata
     3901 cgcagtttgg cgaaccgtcc ttccgccttt ttgggatgga tgcgttcgcc ttcgggatgg
     3961 atttgaatca cgctggcacg cggatcgaac gtttttttca ctttcggcgc ggcttttgtt
     4021 tgttcggacg tgccgattcc ggatgccgga ctgccggctt ggttttccgt ggtcattctg
     4081 cattccttag atttttgatt gatggtttgc ccgttaccgc cgccgttttg cttttcagac
     4141 gtcatttttc ttgttttttt aaggcattgt gtttcaagtt ttgagaaaat ccgcttttcc
     4201 caaaatatat tcccgctatt gtacaacttt atgtgccgtc cggatgtatt gggcgaatac
     4261 atttcccatc cgcatcaaaa cgccctgatt ttaccgcacc gcccgaacaa aattaaaata
     4321 ctcttaaaaa acaaagacta aaaaaaacag acaccccctt atcggcagaa ccgccggcgc
     4381 aagctcataa acaaacgcta tcaacaatcc ggcacacaat ctataacgtt ttatttcaaa
     4441 aggaataatg gcaggcttcg cccgcaaatc gaaaatcctt ccccaccgcc caccccctgc
     4501 cgccgccttc ccccgcgtcc gcccttttct tgaaagcata agcgaatcgg gcgataatca
     4561 acgccttccg attatccact tatccgaaac accagcaagg aaaatacaaa atgtctcaac
     4621 tggcaaacgt aatccgcttc ctctcggccg atgccgttca aaaagccaat tccggccacc
     4681 ccggcgcgcc tatgggtatg gcggaaatgg cggaaacatt gtggacgaaa ttcctcaatc
     4741 acaatcccgc caatcccaaa ttctacaacc gcgaccgctt cgtcctctcc aacggtcatg
     4801 cgtctatgct gttgtacagc ctgctgcacc tgaccggcta caacctgagc attgaagatc
     4861 tgaaaaactt ccgccagctg cacagcaaaa cccccggcca tcccgaatac ggctacaccg
     4921 acggcgtgga aaccacgacc ggcccgttgg ggcaaggtat tgccaacgca gtgggcatgg
     4981 cattggcaga aaaaatcctt gccgccgaat ttaataaaga cggtttgaac atcgtcgatc
     5041 actacactta cgtctttatg ggcgacggct gcctgatgga aggcgtatcg cacgaagcct
     5101 gctcactcgc cggcaccttg ggcttgggca aactgattgt tttgtatgac gataacaata
     5161 tctccatcga cggcaaagtg gacggctggt ttaccgaaaa catcccgcaa cgctttgaaa
     5221 gctacggctg gcacgtcgtt cccaacgtaa acggccacga caccgccgcc attcagacgg
     5281 ccatcgaagc cgcccgcgcc gaaaccggca agccgtccat catctgctgc aaaaccttaa
     5341 tcggcaaagg cagcgccaac aaagaaggca gccacaaaac ccacggcgcg cctttgggtg
     5401 cggacgaaat cgaagccacg cgcaaacatt tgggttgggc ttatcctgcg tttgaaatcc
     5461 cgcaagaaat ttacgatgcg tggaatgcca aagaaaaagg cgcgaaactg gaagccggat
     5521 ggaacgaact gttcgcgcaa tatcaagcca aatatcctgc cgaagccgca gaattcgtgc
     5581 gccgcatgga taaaaagctg ccggaaaact ttgacgagta cgttcagacg gcattgaaag
     5641 aagtgtgcgc caaagcggaa accgtcgcca cccgcaaagc cagccaaaac agcatcgaaa
     5701 tcttggcaaa agagctgccc gaactggtgg gcggttctgc cgacctgacc ccgtccaacc
     5761 tgaccgactg gtcaaacagc gtctccgtta cccgcgacaa aggcggcaac tacatccact
     5821 acggcgtgcg cgagttcggt atgggtgcga ttatgaacgg tttggtattg cacggcggtg
     5881 taaaaccctt cggcgcgact ttcctgatgt tcagcgaata cgaacgcaac gccttacgca
     5941 tggcggcact gatgaaaatc aaccccgtct tcgtattcac ccacgattcc atcggtttgg
     6001 gcgaggacgg cccaacccac cagcctatcg agcaaaccgc caccctgcgc ctgattccga
     6061 atatggacgt atggcggccg tgcgacaccg cagaatctct ggtcgcttgg gcagaagcgg
     6121 caaaagccga agaccacccg tcctgcctga ttttcagccg tcaaaacctg aaattccaag
     6181 cacgcagcga gcaacaactg aacgacatca aacgcggtgc ttacgtaatc agtgaagccc
     6241 aaggcaacgc ccaagccgtc atcattgcca ccggttccga ggtaggattg gctgtagaag
     6301 cgcaaaaagt attggcagga caaggtattg ccgtgcgcgt cgtttccatg ccgtccacca
     6361 gcgtcttcga ccgccaagac gccgcctatc aagccgccgt cctaccggaa ggcctgccgc
     6421 gcatcgccgt agaagccgga cacaccaacg gctggtacaa atatgtcgga ctgaacggcg
     6481 cagtcgtcgg catcaaccgc ttcggcgaat ccgcccctgc cgatctgctg ttcaaagcat
     6541 tcggctttac cgtggacaat gtggttgata cggtgaaatc cgtgctgtaa acctcagccg
     6601 ccgcatcaaa tgccgtctga atgcttttca gacggcattt ttttgccctc ccgccttgat
     6661 gcttcctcat tccgccaatc tccattataa tatttgcgaa tcactcttat tcacatttca
     6721 aaaggagaaa cgtatgaaca cccgcaccga acacgacacg atgggcaatg tcgaagtccc
     6781 gtccgaagcc tattggggcg cgcagaccca gcgcagccgc aacaatttca aaatcggcgg
     6841 cgaaaccctg ccgcagccgc tgatttacgc tttggcgttg gtgaaaaaag ccgccgccgc
     6901 gaccaatgtt tccctcggca ggattaagcc cgaacaggcg gatttgatta cgcaggcggc
     6961 ggatgatgta ctgaacggca ggctcgacgg gcagttcccc ttagtggttt ggcagaccgg
     7021 ttccggtacg cagtccaata tgaacatgaa cgaagtgctg gcaaaccgcg ccaacgaaat
     7081 tgcaggtacg ggtttggcgg cgtaccggcc cgtccacccc aacgaccatg tgaaccgcgc
     7141 gcaatcgacc aacgacgcgt tcccgaccgc catccacgtc gccgccgcga ttgaaatcaa
     7201 ccgccacctt attccggcgg taaaagcctt gcgcgataca ttggacaaaa aagcccaagc
     7261 cttcgccccc atcgtcaaaa tcggccgtac ccacttgcag gatgcgacgc cgctgacttt
     7321 gggacaagaa ttttccggct acgtttccca actcgaccac agcttaggcc gtctgaacga
     7381 cgcgctcaaa ggtttgtacg aactcgcctt gggtggaacg gcggtcggta cgggtttgaa
     7441 cagccatccc gaatacgctg aaatagccgc cgccaaactc gccgaactgt ccggcctgcc
     7501 gtttgtcagc gcgccgaaca aatttgaagc cttgggcgga cgcgatgccg ccgttgccgc
     7561 ttcgggcgca ttgaaaacgc tggcggcaag cctgaacaaa atcgccaacg acatccgctg
     7621 gctggcaagc ggcccgcgct gcggcttggg cgaaatcaaa atccccgaaa acgaaccggg
     7681 ttcgtccatt atgccgggca aagtcaaccc gacccaatgc gaagcgatga cgatggtgtg
     7741 ctgccaagtg ttcggcaacg acgttaccat cggtatggcg ggcgcgtcgg gcaatttcga
     7801 gctgaacgtc tatatgcccg ttatcgccta caacctcttg caatccatcc gcctcttggg
     7861 cgacgcgtgc aacagcttca acgaaaactg cgccgccggc atcgaacccg taccggaaaa
     7921 aatcgactat ttcctgcacc attccctgat gctggttact gcgttaaacc gcaaaatcgg
     7981 ctacgaaaac gccgccaaag tcgccaaaac cgcctacaaa aacgataaat cgctgcgcga
     8041 aactgccgtt gggttgggct tgctgacggg cgaagagttt gacgaactgg tcgtccctgc
     8101 cgatatggtt catccgcgct aatccttccc tcaaataaaa tgccgtctga agcctcgttc
     8161 agacggcatt ttccgttgcc tgcgaactag cggcgtttga acggcctgtc ccccaccgcc
     8221 gccgtaaccg cacccccgac cacgacgagt gcgccgacat aacccaaacc gttcatatcc
     8281 ggcgcggcaa aagtatcagg catcacataa tgcccgagca aagaaaatat tacggtaaac
     8341 acggggagca aggttgttac cgcgctgact ttggaagcct cccaatgttt caacgcctcg
     8401 ccgaacgagc cgtaaccgat taacgtattc aagcagcaat acacaaaaca aacccacgcc
     8461 aacgtaccgt ccaaacttcc gatgtgtgcc ggttcggcaa acggcaggaa cacggcggca
     8521 cttgccgcat aaatcaacag cagaatctgt tgcggaccga attgcgccga cagcagcttt
     8581 tgcgccacgg cataacacac ccaggccata ctgcctgccg cacacagcaa cacgcccttc
     8641 gcatacgcgc ccaaacccga caactcgccg aatttgtcgt taaaaaacat aagcaaaccg
     8701 acaagcagca aaaccaaacc gattttctgc gcggcagtca tccggtcttt aaacaccaac
     8761 acgccgacaa caatcatcgt aaacggcgaa atctgccaca aaacctgcgt cgtggtcggc
     8821 gaaatataat gcagcccttg ggcaatcagc acaaagtttg ccgaaatgcc cgtcacgccg
     8881 agcagcagca gcctgaatga atgccaagaa aaatcccgcc gcttcggcag ccgcccgccc
     8941 aatgtcagca aaacaaacaa taccgccgcc gccactgtaa aacgcaccca caccagcgtc
     9001 ggcgcatcga caaacttcaa tacctgccgc acggcaatcg gcagcatccc ccacgtcatc
     9061 gccgccaaaa gtgccaacgc gaagcctagg agcggccttt ggttttccat cctgatttcc
     9121 ctatttttaa acaaccgtat tgccggacga tgccggtttg ccgcatcggg caatgatggt
     9181 tcaagcgttt ggcgtttgat tccaaccctt tgatttcaaa caaactggct gaagcccgac
     9241 tattgcttcg cactatttga aaacaccgcc tcaattttaa aatgccgtct gaaaacagcg
     9301 ggggagtgcg gcaggcatcg cttgcctcaa aacgcccgaa cggcgcaatc gcaacgttac
     9361 gcccaacgca gggggcggca acaagccggc ccaaatgcaa aaaagagaaa ccttgccccg
     9421 taaggtttaa ggtttttccg tcctttatga cttccctccg cgaggatgtc cggccgtaaa
     9481 attcagaacg ggatgtcgtc gtcaatgtcc tcgaccgggg cggcggccgg cacgggttgg
     9541 cggcgcggcg cggcgggtgc ttcttgggga tgggacggcg cgtcgggagc gggctgccga
     9601 ctttgctgag cggggcgttg gtaagcctcc tgattctgac cgtaaccttc atcgtaaggc
     9661 gcgccgccgc tgttttcatt gcgcccgccc aacattttca tttcattggc gacaatatcg
     9721 taagcggtgc gttcgatgcc gtctttgcct tggtatttgc ggctttggat tctgccttcc
     9781 agataaacca gcccgccttt tttgaggtat tgcccggcaa tttccgccag tttgcggtac
     9841 atcgtgatgt tgtgccattc ggtgcgctct acgcgctggc cgttgcggtc gttccaagtt
     9901 tcgctggtgg cgacgctgaa attacacact gcctcgccgt tgggcatata gcgcacttcg
     9961 ggatcgcgtc cgaggcggcc gataaggatg actttgttca atgacatttt ttaaactcct
    10021 gtaatgattt tttcagcggc agcctgatcg aaacccttct gcaacacttt aagatagacg
    10081 gtctgcccgt cgaaactgaa accgatgtct tccacgcctt ccaactgcaa cagggcgcgt
    10141 tgcaaaccgt cccgattgcc ctgccacacg ccgccgacag ggtaactgag gtttttgacg
    10201 ggcttgggcg cgggcgataa aacggcaatc accagccaca gcagcatcaa tatgctgcaa
    10261 aaggcaaaca cgccggcaaa gccgtatttt tgaaacagca agcctcctgc cgcgccgccg
    10321 gcaaacagtc cgagcgactg catcgtgttg tacacgccca tcgccgtacc cttcaggtcg
    10381 gacggcgcga ttttggaaac catagacggc aggctcgctt ccaacacatt aaaaccgata
    10441 aagtaaacaa ccagataagc ggtaatcagc caaatcgagt gcattccgga cagcaaacca
    10501 agctgcgccg ccgcgatgca gacgatgccc aaaacaaaaa cctgtttgag cttgttgcgc
    10561 gtttcgccga cgataatcag cggaaccatc accaccaagc ccgtaatggt cgagggcaga
    10621 tatactttcc aatgctggat tttttccaaa ccgagccggg tcatcgcgaa aggcagcgcg
    10681 gtaaacaatg ccatttgcgc ggcgtgcagg gcgaaaatgc cgaaatcgag cgtcagcagc
    10741 ctacggtttt tcaaaacttc tcctatgcgc gaaggctgcg cctgcgtgtc ctcatgcagc
    10801 ttggaaactt cgggatcagg agtcatccac gccactacgc cgatgctgat gacggtcaga
    10861 atgccggtca gcataaacag tccgcgaacg ccggccacat cggcaatcat cggcgcaacc
    10921 accaggctga ccgaaaacgt cagaccgata ctcaaaccga tcatcgccat cgcgcgggta
    10981 cgcacgccgt cgcgcgtcaa gtcggcaagc agcgcggtta cggcagcact gaccgccccc
    11041 gcgccctgta tggcgcgcgc ggcaaccagc atgggcagcg tatcggcggc ggcggcaagg
    11101 aagctgccgg cggcaaacac gaccagcccg acataaatgg ttttcttgcg cccgaacttg
    11161 tcggaagcga tacccaaagg cagttgcagc aatgcctgcg tcagcccgta aatgcccatt
    11221 gccagcccga ccagcgtttt attgtcttcc gcaccgggca gcgaggcggc atatattgcc
    11281 aaaacgggca gcacgaggaa catacccagc atacgcagcg cgtacacgcc ggaaagcgtc
    11341 gtactggcgc gccattcgtg cggaaacatt tggatgcggt tgtctcttgc cataaataat
    11401 ttttcagacg gcatcaacag ttgcaatgcc gtctgaactt ccagtgaaca gattttcgga
    11461 ttatacagga ttcgccgtat ttcggttgcg gcacgggttc aaaatcaacg ccactgccag
    11521 cggttgcgcc acgcgcccaa aacggcgttc ggatatttat tgctgccgag gctgccgttg
    11581 aagcgggcaa gtgcgcggac gatgtcgccc ttttcaaggt ttcggtaatg gcgcaggatg
    11641 gtgcagccgt aacgcaggtt ggtacggata tcgaacaggt tgtgcgccgg tttgccgatg
    11701 tagtttttcc aaaacggcat aacctgcatc agaccgcgcg cgccgacccc gcttatcgca
    11761 tactgccgga acgcgctttc cacttcaatc agccccaaca caatctgcgt atccaaaccg
    11821 gcccggctgc tttcgtattg gatattgacc agcagcctgc gccgctcccc ctcgtcgggg
    11881 acgaatcttg ccaaacgtgc cgacatcgcg gacaaccaac gttcgccctc tttcggattg
    11941 tcgaacacca gcctcggcgg attgacgctg ccgacagaac tcctcatcac ggaagccaca
    12001 tcgtcggcaa gcgtttcttc acgttgcgcg ccggcgtgcg ccagcgggct gatcaacagc
    12061 gcgccggcgg cacacagcag gcggcggcgt tgcagattga cgggtagggt atcggtcggt
    12121 tttttcataa ggaacggggg tgcgtccgga cgtttcagac ggcattaaat attcaaacag
    12181 acatgattgc ttccaacgcg aaaaaccgcg cgcaaaatcc aagcgcggca tatcgccctg
    12241 cccttttcgg gcaaaccttg attttaccgc cctcaagaat gcttgtccaa acaggcacag
    12301 acgacaccgc ccgggcattt cccgttttca ccggttatcc gtcgtccgga ttgtgcagca
    12361 gcaccatcag cgcattacgc ttttcgggcg gcagcaggcg gaaatatagt ggattaaatt
    12421 taaaccggta cggcgttgcc tcgccttgcc gtaccggttt aaatttaatc cactatatct
    12481 tgagaccttt gcaaaattcc cttccctccc gacagctgaa accctgacac agattttcgg
    12541 ctgtttccgc caccaatcac tcctaactct acccaaatac ccccttaatc ccccttgaat
    12601 acccgataat caggcatccg ggccgccttt taggcggcag cgggcgcact tagcctgttg
    12661 gccgctttca acaggttcaa acacatcgcc ttcaggtggc tttgcgcact cactttaatc
    12721 agtccgaaat aggccgcccg ggcatagcgg aatttacggt gcagcgtacc gaagctttgt
    12781 tcgaccacat aacgggtctt cgacaaatat cggttgcgtt tggtttgcgc ctccggcagc
    12841 gggcggttgc ggcgggcttt gcgcataatg ccgtccagca gccgatgctc tttcagtttt
    12901 ccgtaggtcg gattctcgaa tccgacatta cttcaatcgt attcaataga aaagtccgca
    12961 ttgccgccac cccaattatg cggataaata ccctgtttga cataacggtg aaacgtagaa
    13021 aaccgccaat cggaaatttg tcctacatag ccatgtttga ccggattgaa atgcagataa
    13081 tcaaaatggc aggcaaaatc ggcctcaccg cggatagtat gttcccaaaa gcgtttttcc
    13141 aaagcctgag attgccgccg attaaatatt ggctgtgccg cttgatttgc cgccagcgtt
    13201 ccgaataagc agaatcattg tccggcagcc gccatatggt atgcagatga tcgggcatca
    13261 acacccatgc caaaatttca aacggattcc gttcgcgcac cgccattacc gcctgccgta
    13321 aagccaaacg caccgcatca tcggtcaaaa tcttctgccg tttattggtt acaaccgtaa
    13381 aaaagtaagt gccgccattg cggtaaaaac gacggtattt catagtatta tgctcggaat
    13441 gattttgtag gccggattct tgaattcgac attttggaca ttgctgcaat ggattgcaat
    13501 gatgggaatg ttaaaggttt tgtcggatac aagtatccga cctacgcttg ctgaaccgtc
    13561 attcccacgg aagtgggaat ctaggacgcg gggtttcagt catttccgat agattcccgc
    13621 cgcgtcgggg gtccggattc ccgcctgcgc gggaatgacg aatttcgaga ttgcggtatt
    13681 gtcgggaatg acggttcggg tatttccaag cccgccccgc gcctgtaaac ggcgggcgat
//
LOCUS       DAXDSD010000064        13445 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXDSD010000064 DAXDSD010000000
VERSION     DAXDSD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.064"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(263..1198)
                     /gene="hemC"
                     /locus_tag="ACFB2Q_001426"
     CDS             complement(263..1198)
                     /gene="hemC"
                     /locus_tag="ACFB2Q_001426"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC0918433.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            1317..1832
                     /gene="ybeY"
                     /locus_tag="ACFB2Q_001427"
     CDS             1317..1832
                     /gene="ybeY"
                     /locus_tag="ACFB2Q_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC0918434.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENVSSAAGIPAERDFYRWAWSAL
                     KNEYLRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            1834..2658
                     /locus_tag="ACFB2Q_001428"
     CDS             1834..2658
                     /locus_tag="ACFB2Q_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC0918435.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2801..4180)
                     /gene="nhaC"
                     /locus_tag="ACFB2Q_001429"
     CDS             complement(2801..4180)
                     /gene="nhaC"
                     /locus_tag="ACFB2Q_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC0918436.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTACATVGVAFMGMAAAFQADMAMTAGAIV
                     SGVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4470..5693)
                     /locus_tag="ACFB2Q_001430"
     CDS             complement(4470..5693)
                     /locus_tag="ACFB2Q_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC0918437.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            6130..6543
                     /locus_tag="ACFB2Q_001431"
     CDS             6130..6543
                     /locus_tag="ACFB2Q_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC0918438.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            6590..7219
                     /gene="nth"
                     /locus_tag="ACFB2Q_001432"
     CDS             6590..7219
                     /gene="nth"
                     /locus_tag="ACFB2Q_001432"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC0918439.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            7357..8856
                     /locus_tag="ACFB2Q_001433"
     CDS             7357..8856
                     /locus_tag="ACFB2Q_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC0918440.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            9059..10579
                     /locus_tag="ACFB2Q_001434"
     CDS             9059..10579
                     /locus_tag="ACFB2Q_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC0918441.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRADV"
     gene            10636..11721
                     /gene="nagZ"
                     /locus_tag="ACFB2Q_001435"
     CDS             10636..11721
                     /gene="nagZ"
                     /locus_tag="ACFB2Q_001435"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC0918442.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDSEGAET
                     AETHAEHIGWVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIILVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            11946..12022
                     /locus_tag="ACFB2Q_001436"
     tRNA            11946..12022
                     /locus_tag="ACFB2Q_001436"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11980..11982,aa:Pro,seq:cgg)
     gene            complement(12358..12996)
                     /locus_tag="ACFB2Q_001437"
     CDS             complement(12358..12996)
                     /locus_tag="ACFB2Q_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC0918443.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGRNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLKPAPPQIDYVATSPKACYAAKYETNCIAEADEVRIVADGDVLAFCENMERK
                     IRARHYYLSPCEQNGVMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(12993..13361)
                     /locus_tag="ACFB2Q_001438"
     CDS             complement(12993..13361)
                     /locus_tag="ACFB2Q_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC0918444.1"
                     /translation="MKLLSTILVLLVAAEHFYIAWLEMTQIPGEKAAEMFKLPYEFME
                     QKRVQTLFGNQGLYNGFLAVGLLWAQFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAVLAV"
BASE COUNT         3376 a         3831 c         3382 g         2856 t
ORIGIN      
        1 aaaaaatacg aatatcatca ttctcaaatt caaaatttga acgccacagt attatccgaa
       61 gccacaaaaa cctttccgcc gtcattcccg cgcaggcggg aatctggaaa cccaatgctg
      121 caagaattga ttaaaaacaa ctgaaaccga gcaaactgga ttcccgcctg cgcgggaatg
      181 acgaaataag gtaagtagaa cttattaaat aaattaatta aaaacaataa cttatattat
      241 tttctttaaa ttttcgataa aatcaatttt ccgtattcaa tactgctcca atcaattcct
      301 gcgcaccgtc gtccgccagt tttttggcaa ccgcacgtcc aagcgcatcg gcatatccgg
      361 caggggcttg cgcgtccgcc tgcaacacaa tcgatccgtc ggggtgcccg accaatccgc
      421 gcaagatcag cagcccgttt tcttcagtgc aatacgcggc cagcggcact tggcagcttc
      481 cgcccaaagc gcgcgccaaa gcgcgttcgg cggtaacgca ggcgtgtgtg gtatcgtggt
      541 ttaacggctt caagacttcg tacaaatctt cgcgatgcgt ggcaatttca atacccaatg
      601 cgccttgtcc ggcggcaggc aggctgtcgg attccgacaa aatcatgcgg atgcgttcat
      661 ccaattccag acgctgcaac ccggcggcag ccaagataat tgcatcgtat tcgccgttgt
      721 cgagtttgga caaacgggtt tgcacattgc cgcgcaaagg tttgataagc aaatgcggat
      781 agcgcgcgcg caactgggct tcacggcgca ggctggatgt gccgacaacc gcgccttcgg
      841 gcatttcttc caaacgcgcg tatcggttgg acacaaacgc gtcaaacgga ttggcgcgtt
      901 cgctgatggc ggcaagggcg aagccttcgg gcaaatccat cggcacgtcc ttaatcgaat
      961 gcaccgccaa atcggcgcgc ccgtcttgaa gggattgttc caactctttg acaaacaagc
     1021 ctttcccgcc gacttttgac aaagttctgt ccaaaatccg gtcgccgcgc gtggtcatgc
     1081 ccaaaatttc gacttcgcaa tcgggataca gcgccttcag acggccttgg atatgtttcg
     1141 cctgccacat ggcaagcagg ctttcgcggc tggcgataac aagttttttc gggttcatgt
     1201 tcaatgcgtt caaagaatgg aaacggcgtg gagtctatca tattttgaac gtttcttcag
     1261 acggcaccgt caggtctatg ttatatttac gcccacaaaa cacgaaaccc gccacaatga
     1321 aacgcgccaa aaaataccct ttcttaacgt tgcagcggca acgtttccat ttgaactttg
     1381 aaaacgtctc ttccgccgcc ggcatccccg ccgaacgcga tttttaccgc tgggcgtggt
     1441 ctgccttgaa aaacgaatac ctccgcgccg acatcggttt gattcttttg gacgaagaag
     1501 aagcccgcgc ctacaaccgc gactaccggg gcaaagatta tgccaccaat gtattgagtt
     1561 tcgcgctcaa cgaaggcgaa atcctgccct gccaagtttc ggaaagactg tacggcgatt
     1621 tgattatctg cccgcaagtc gttttaaaag aagccgccga acagggcaaa acgcctgaac
     1681 ggcattttgc ccacttaacc atacacggca cgctgcacct gatgggatac gaccacatca
     1741 aagacgatga ggccgaaata atggaagccg aagaaatccg cctgatgcgg gcggcaggct
     1801 atcccaaccc ctaccgagag gacggacatt aaaatggacg gcgcacaacc gaaaacaaat
     1861 ttttttgaac gcctgattgc ccgactcgcc cgcgaacccg attccgccga agacgtatta
     1921 aacctgcttc ggcaggcgca cgaacaggaa gtttttgatg ccgacacact gacccggctg
     1981 gaaaaagtat tggactttgc cgagctggaa gtgcgcgatg cgatgattac gcgcagccgc
     2041 atgaacgtat tgaaagaaaa cgacagcatc gaacgcatca ccgcctacgt catcgatacc
     2101 gcccattcgc gcttccccgt catcggcgaa gacaaagacg aagttttggg cattttgcac
     2161 gccaaagacc tgctcaaata tatgttcaac cccgagcagt tccacctgaa atccgtcttg
     2221 cgccctgccg ttttcgtgcc cgaaggcaaa tctttgaccg cccttttaaa agagttccgc
     2281 gaacagcgca accatatggc aatcgtcatc gacgaatacg gcggcacgtc gggtttggtc
     2341 acctttgaag acatcatcga gcaaatcgtc ggtgacatcg aagacgagtt tgacgaagac
     2401 gaaagcgccg acaacatcca ctccgtttcc gccgaacgct ggcgcatcca cgcggctacc
     2461 gaaatcgaag acatcaacgc ctttttcggt acggaatacg gcagcgaaga agccgacacc
     2521 atcggcggct tggtcattca ggaattggga cacctgcccg tgcgcggcga aaaagtcctt
     2581 atcggcggtt tgcagttcac cgtcgcccgc gccgacaacc gccgcctgca cacgctgatg
     2641 gcgacccgcg tgaagtaagc agagcctgcc cgcaccgccg tttctgcaca gtttaggatg
     2701 acggtacggt cgttttctgt ttcaatccgc cccatccgcc aaacataaaa atgccgtctg
     2761 aaaccggaaa acaggttcag acggcatttc gcttatccgc ttattttttg ctcaaagtca
     2821 gccccgtcca gccgaataac agggttaaag ccaaactcaa atagcagaaa aaggcataag
     2881 gcagatattc ccaaacgggt acgccaaggg cgtggctgat aaatacgccg cacacgctcc
     2941 acggcacgag cgggttaatc accgtccccg catcttccag agtccgcgac aggttgcgcg
     3001 aatgcaggcc gagtttgtcg taaacgggtt tgaacgtttc tcccgaaagc aggatgctca
     3061 aatattgctc tccaatcagg aaattgaccc cgaccgaagt catggcaacg ctgaacgtcg
     3121 cgcgtccggc attcgtcaag aaggtacgga cggcctccag cagggaagga atcacaccga
     3181 gcgcaaacag cagcccgccc aaactcatac cgaggataac gatggtctgc gtaaagaaca
     3241 tactctccaa gccgccgcgc gaaatcagtt tggcaatgtc tttaaacgct tcgccttcga
     3301 gtttgtagcc gccataaaac cacgcgccga gctgacgcag atcgggcgtg ctgtgcagat
     3361 acgttacggc aacggcggca atgacggtaa agagcatggc gaccacggca ttgacgcgca
     3421 tcaatgccaa aacgaccaac agtgcaaacg gaatcagcga atagccgtgc accaatcccg
     3481 tggcttcaag ctggctgcgg aaggattcga cgctgttcaa atcctgcgcg gcgacgctgg
     3541 gaagaagcca aagcatcagt gccgcgctga taagccacgc ggggatggtg gtgtacatca
     3601 tgtttttgat gtgttcaaac aggtcgatac cgacgatgga cgcggaaatg cccgtggtgt
     3661 cggaaagcgg ggacatttta tcgccgaaaa acacaccgga aacaatcgcg cccgccgtca
     3721 tcgccatatc ggcctgaaac gccgccgcca tccccataaa ggcaacgccg acagtggcgc
     3781 aggcggtcag gctgctgccg atggacacgc cgatgacgga acacagcgcg aaggcggaaa
     3841 aataaaaata agtcggggaa atcagcccga aaccgtaata catcagcgtc ggaatcgcgc
     3901 cgctcatcat cagcgcgctg accatcagcc cgatgaagaa aaacaggtaa accgcgccca
     3961 taccctgatt caacgcgcct atcatccctg cctgcatatc gttgtatttc aaaccgcgcg
     4021 ccaagccgta caaaatcagc acgacgatgg cggcaataat ggacatatgc ggcagccact
     4081 ccaatgaaat gatggtatag cccattgcgg caatcagagc gacgacgacg gcaagtgcct
     4141 caccgcgcgg catatcgagt aaggatttga aagcaaacat gattctttgc ccgatttgat
     4201 ataaagttaa cggattctaa cacaccatgc cgaatctgcc cgacgttttc agacggcatt
     4261 ttagcgtttt acaaaagccg ggcagaaaca aaacggcaat tttctcgatt taaattaatt
     4321 tttttgaaat ataattttta tttttttatt tgttggcata taagactttt atggattgaa
     4381 gtattaaaac ctcggcacac acgccgcaaa caccggtacg caaaaatgcc gtctgaaaga
     4441 tgttcagacg gcatttctca agaaaaagat tatttgtccg ccctatatcc gtacaccgca
     4501 aaaaacacga tatacaggta acagataacg gaaacgacaa acgaagacat cagggtgtaa
     4561 gtatccgccg cccaaccttg aattaccggc acgaccgcac cgccgacaat cgcggtacac
     4621 agcacaccgg aagccgcgtt ggtgaatttg cccaaccctt tggttgccaa agagaaaatc
     4681 gtcgggaaca taatcgagtt gaaaaaaccg atggcaagca gcgaccacat cgccacatcc
     4741 gcattgccgc tgcccgtcgc catcgcgacg gcaagcagca cgaccgcagc cgatgcgtta
     4801 aacgccaaat aacggttggg cgcgaatttc gccattaccg ccgaaccgag gaaacgtccg
     4861 accatcgctc cgccccaata aaacgacagg taatgcgcgg cagaagcatg atccagccct
     4921 ttcagataac ccaacacatt gaccatcagc gaaccgatag acacctccgc accgacgtag
     4981 cagaaaatgc ctgccgtacc gaacacgaga tgtttgtatt gccatacgcc ggttttgccg
     5041 tcgtggttgt gcgcgctttc ctcggcggca attttgcgcg cgtcgggcag ccggatcatt
     5101 ttcacgaaaa cggcaaggat aatcagcagc cccgccagtc ccaaataggg aatctgtacg
     5161 gaagaaatct gttccgcctt gctgacggtt tgggttgcgt ccgccaaaat caggaacgcg
     5221 ccgatttgag gcgcaatggt cgtacccaaa gcgttaaacg cctgaaccag cgtcagcgtt
     5281 gccgattcct tgccgggttt cgccagcagg gtaacataag gattaccggc gacctgaagc
     5341 agcgttacgc cggaagccaa aataaacaac gcgcccaaaa ataccgcgta agaatggctg
     5401 cccgcagcag gataaaacag caggcacccg accgccgtca gcagaaagcc gccgataacg
     5461 ccgtttttgt agccgacttt gccgacaaaa gcccccatcg ggatggacat taccgcatag
     5521 gcggtaaaaa agcaaaattg aatcagcatc gcctgaacgt aagagaggtc gaaaatttct
     5581 ttcaaatgag ggataaggat gtcgttcatg caggtaataa aacccatcat gaagaacagc
     5641 gtggtcaaga cgaccaatgg ggacgtatgg ttgttttgtg attgcgcaga catgatttgc
     5701 ctttcgtggt gtcggaatat tgcccgtttc aagaaaatag aaattgacat ttcttaacgg
     5761 caatgcaaat catactgata tttgaacatt ttgcaaactt tatcggatgc aggcaatgcg
     5821 ccgatctgaa attcaagcag cagaaattaa tattttacag ttgtttgcat aggttttaat
     5881 agattttaaa aaattccggc gggcttgcaa aaaccgttcc caataaaatg aaaatcctcc
     5941 gccgtatccg gaaaattcgg aaatgccgca tcggggcatc aaaaccggag cggaaagaat
     6001 gtcgtatgcc cgacaaataa aggggcatac ggctgtgccg tatttttgca ccgtcctccg
     6061 cccttcggcg ggggcgtgaa aataccgtat aatccgtcgc cgatttttac ctgtttggga
     6121 ctgtttgcaa tgataccctc ctacacctac gccgccctcg ctttttccgc cttcacttcc
     6181 gccaccctcc tgcccggcac gtccgaagcc gccttcgccc tgttcgtccg caacttcccc
     6241 aaacacgcat acggcgcgtt gctgtgcgcc ggccttgcca acggattggg cagtatggtt
     6301 tcctattgga tgggacgttt gctgccttcc cgaaaaatgc cgtctgaaaa aacactgaat
     6361 ctgatacggc gtttcggtat ttggctgctt gcgtttgcgt ggctgcccgt cgtcggcgac
     6421 gcactgccgc ttaccgccgg ctggctgcgg ttgaatccgt ggacaagcgg gctgatgctg
     6481 gttatcggca aaacggcgcg ttacgccttc attctgtggg gaatgcaata ttacgccgcc
     6541 tgaaccgtgc gttataatgc cgaccgcaca aaccgactgc acaaaaaaaa tgaacagaca
     6601 aatccgccaa gaaatcttcg agcgtttccg cgccgccaac ccccatccga ccaccgagct
     6661 gaatttcaac tcccctttcg agcttttaat cgccgttctg ctttcggcgc aggcgaccga
     6721 tgtcggcgta aacaaggcga cggcgaagct gtttccggtt gccgatacgc cgcaggcgat
     6781 gctggatttg ggcttggacg gcgtgatgga atacacgaaa accatcgggc tgtataaaac
     6841 caagtccaaa cacattatgc aaacctgccg catcctgctg gaaaaataca acggcgaagt
     6901 gccggaagac cgcgaggctt tggaatcgtt gcctggcgtg gggcgcaaaa cggcaaacgt
     6961 ggtattgaac acagcgttcg gacaccccgt tatggcggtc gatacgcata ttttccgcgt
     7021 gtccaaccga accaaaatcg cacccggaaa agatgtacgc gaagtcgaag acaaactgat
     7081 gcgcttcatt cctaaagaat ttctgatgga cgcgcaccac tggctgattt tgcacggacg
     7141 ctacacctgc aaggctttaa agccgcaatg ccaaacctgc atcatcaacg atttgtgcga
     7201 atatcccgcc aaatcctgac cgccgccgcc cttcaaaatg aaacgcttga aaattcggac
     7261 ggacgttgta gaatttcaac ctatgccgtc tgaagccgac aaaatgcgct tcagacggca
     7321 ttaaagccgg taccggcatc cgacaaagga caaaacgtgt tcaaaaaata ccaatacttc
     7381 gctttggcgg cactgtgtgc cgccttgctg gcaggctgcg aaaaggcagg cagctttttc
     7441 ggtgcggaca aaaaagaagc atccttcgta gaacgcatcg aacacaccaa agacgacggc
     7501 agtgtcagta tgctgctgcc cgactttgcc caactggttc aaagcgaagg cccggcagtc
     7561 gtcaatattc aggcagcccc cgccccgcgc acccaaaacg gcagcggcaa tgccgaaacc
     7621 gattccgacc cgcttgccga cagcgacccg ttctacgaat ttttcaaacg cctcgtcccg
     7681 aacatgcccg aaatccccca agaagaagca gatgacggcg gattgaactt cggttcgggc
     7741 ttcatcatca gcaaaaacgg ctacatcctg accaataccc acgtcgttgc cggtatgggc
     7801 agtatcaaag tcctgctcaa cgacaagcgc gaatataccg ccaaactcat cggttcggat
     7861 gtccaatccg atgtcgccct tctgaaaatc gacgcaacgg aagagctacc cgtcgtcaaa
     7921 atcggcaatc ccaaaaattt gaaaccgggc gaatgggtcg ctgccatcgg cgcgcccttc
     7981 ggctttgaca acagcgtgac cgccggcatc gtgtccgcca aaggcagaag cctgcccaac
     8041 gaaagctaca cacccttcat ccaaaccgac gttgccatca atccgggcaa ttccggcggc
     8101 ccgctgttca acttaaaagg acaggtcgtc ggcatcaatt cgcaaatata cagccgcagc
     8161 ggcggattca tgggcatctc ctttgccatc ccgattgacg ttgccatgaa tgtcgccgaa
     8221 cagctgaaaa acaccggcaa agtccaacgc ggacaactgg gcgtgattat tcaggaagta
     8281 tcctacggtt tggcacagtc gttcggtctg gataaagcca gcggcgcatt gattgccaaa
     8341 atccttcccg gcagccccgc agaacgtgcc ggcctgcagg cgggcgacat cgtcctcagc
     8401 ctcgacggcg gagaaatacg ttcttccggc gaccttcccg tcatggtcgg cgccattacg
     8461 ccgggaaaag aagtcagcct cggcgtatgg cgcaaaggcg aagaaatcac aatcaaagcc
     8521 aagctgggca acgccgccga gcataccggc gcatcatcca aaacagatga agccccctac
     8581 accgaacagc aatccggtac gttctcggtc gaatccgcag gcattaccct tcagacacat
     8641 accgacagca gcggcaaaca cctcgtcgtc gtacgggttt ccgacgcggc agaacgcgca
     8701 ggcttaaggc gcggcgacga aatcctcgcc gtcgggcaag tccccgtcaa tgacgaagcc
     8761 ggtttccgca aagctatgga caaggcaggc aaaaacgtcc ccctgctggt catgcgccgt
     8821 ggcaacacgc tgttcatcgc attaaacctg caataacgca cataatttaa ccaaaatatt
     8881 tttaaatgat aaaatgcccg tcaataccga ttaacgggca ttttacaaaa atcagttggt
     8941 tgattttaaa caaatatttt ttacgacaaa aaatatttcc acccattttg ccaacaaaac
     9001 ccaaaccgga ggcggacaga tacccaccca tccggacgct taacaccccc cataaaccat
     9061 gcagctgatt gactattcac attcattttt ctcggttgtg ccaccctttt tggcactggc
     9121 acttgccgtc attacccgcc gcgtactgct gtctttaggc atcggtattt tggtcggcgt
     9181 tgcctttttg gtcggcggca accccgtcga cggtctgaca cacctgaaag acatggtcgt
     9241 cggcttggct tgggcagacg gcgattggtc gctgggcaaa ccaaaaatct tggttttcct
     9301 gatacttttg ggcattttca cttcactgct gacctactcc ggcagcaatc aggcgtttgc
     9361 cgactgggca aaacggcaca ttaaaaaccg gcgcggcgcg aaaatgctga ccgcctgcct
     9421 cgtgttcgta acctttatcg acgactattt ccacagcctc gccgtcggtg cgattgcccg
     9481 ccccgttacc gacaagttta aagtttcccg cgccaaactc gcctacatcc tcgactccac
     9541 tgcctcgccc atgtgcgtgc tgatgcccgt ttcaagctgg ggcgcgtcga ttatcgccac
     9601 gcttgccgga ttgctcgtta cctacaaaat taccgaatac acgccgatgg ggacgtttgt
     9661 cgccatgagc ctgatgaact attacgcgct gtttgccctg attatggtat tcgtcgtcgc
     9721 atggttctcc ttcgacatcg gctcgatggc gcgtttcgaa caggctgcgt tgaacgaagc
     9781 ccacgacgaa accgccgctt cagacgctac caaaggtcgt gtttacgcat tgattattcc
     9841 cgttttggcc ttaatcgcct caacggtttc cgccatgatc tacaccggcg cgcaggcaag
     9901 cgaaaccttc agcattttgg gggcatttga aaacacggac gtaaacactt cgctggtatt
     9961 cggcggcact tgcggcgtgc ttgccgtcgt cctctgcacg ttcggcacga ttaaaaccgc
    10021 cgattatccc aaagccgtgt ggcagggtgc gaaatccatg ttcggcgcaa tcgccatttt
    10081 aatccttgcc tggctcatca gtacggttgt cggcgaaatg cacacgggcg actacctctc
    10141 cacgctggtt gcgggcaaca tccatcccgg cttcctgccc gtcatcctct tcctgctcgc
    10201 cagcgtgatg gcgtttgcca caggcacaag ctgggggacg ttcggcatta tgctgccgat
    10261 tgccgccgcc atggcggtca aagtcgaacc cgcgctgatt atcccgtgta tgtccgcagt
    10321 aatggcgggg gcggtatgcg gcgaccactg ttcgcccatc tccgacacga ccatcctgtc
    10381 gtccaccggc gcgcgctgca accacatcga ccacgttacc tcgcaactgc cttatgccct
    10441 gacggttgcc gccgccgccg catcgggcta cctcgcattg ggtctgacaa aatccgcgct
    10501 gttgggcttt ggcacgaccg gtattgtatt ggcggtgctg atttttctgt tgaaagataa
    10561 aaaacgcgcc gacgtttgac cttcccccct gttcagacgg catatgccgt ctgaagtttt
    10621 ttggagcaaa ccgcaatgac cgtcccccat attccgcgcg gccccgtcat ggcagacatt
    10681 gccgccttcc gcctgaccga agaggaaaag caacgcctgc tcgatcccgc aatcggcggc
    10741 atcatcctct tccgccgcaa cttccaaaac atcgaacaac tcaaaacact caccgccgaa
    10801 atcaaagccc tgcgcacacc cgaactcatt atcgccgtcg atcacgaagg cggcagggtg
    10861 caacggttta tcgaaggttt tacccgcctg cccgcaatga gtacgctcgg cgagatttgg
    10921 gacagtgaag gcgcggaaac tgccgaaact catgccgaac acatcggatg ggttttggca
    10981 accgagcttt ccgcctgcgg catcgatttg tccttcacgc ccgtcttaga tttggactgg
    11041 ggaaactgcg ccgtcatcgg caaccgcagc ttccaccgca atcccgaagc ggtcgcccgc
    11101 ctcgccctcg cccttcaaaa aggtttggca aaaggcggta tgaaatcatg cggcaaacat
    11161 ttccccggac acggatttgt cgaaggggac agccatttgg tgctacccga agacgggcgc
    11221 agcctcgacg aactcgaagc cgccgacctc gcccccttcc gcattatgag ccgcgaaggt
    11281 atggcggcgg tcatgcccgc ccacgtcgtt tatccgcaag tggacacaaa gcccgcaggg
    11341 ttttccgaaa tctggctcaa acaaattttg cgccgcgata tcggattcaa aggcgtgatt
    11401 ttttctgacg atttgaccat ggagggcgcg tgcggggcag gcggcatcaa agaacgcgcc
    11461 cgcatttcct ttgaggcagg ttgcgacatc atattggtct gcaaccgtcc cgacttggtc
    11521 gatgaattgc gcgacggttt caccattccc gacaaccagg atttggccgg acgttggcaa
    11581 tacatggaaa actcgctcgg tcatgaggcg gtacaagccg ttatacagac aacgggtttc
    11641 caagcggcgc aagcctttgt tgccggcctt gcctcgccgc aggacacggc gggcggcgta
    11701 aaagtcggag aggcctttta aaccggctgc gtttcaacaa tctgccgatt tcaaccgttc
    11761 gtcagggaaa ggcaatgatt ttttcggtca aatagaaagt tatcttattg aattactttg
    11821 agataaggat gcttatcaat actgtattca aacacagccc tttaaatccg cccgacacgc
    11881 ggcaaaaagg caaaacggta taggcacgcc gtattttttg cgctataatc acaccttaat
    11941 taattcggaa tgtagcgcag cccggtagcg cacttcgttc gggacgaagg ggtcggaggt
    12001 tcgaatcctc tcattccgac catattcaga ccgcctcgac atcgattgcc ggggcggttt
    12061 ttcccttttg gcggcaacaa aaaaatgccg tctgaaacag aatgctgttt cagacggcat
    12121 ttcgttttcc ccaatcctgc catttgcgcg cactgacaaa gggtatcggc aaggttcaaa
    12181 agaccatcgg aatctaggaa gcctggcgtg tacccaaccg cggcatctta ttcattaagg
    12241 cttcgtcctg attgggcggc ggaaatgtcg gcagtttcac gcggaagcgg cacactttcc
    12301 ccgtatgtca aacatcggca agaaggtacg cctgcccttt gggttacact gcttaaacta
    12361 ctctatcccc gcccatttgt gcgtctgcac gctcaactgc caatgcacgg gcgcgtcggg
    12421 gcggctgttt aaaataccga tttggcggat ggtgtcgtag atgttcatca cgccgttttg
    12481 ttcgcaggga gaaagatagt aatggcgcgc acggattttg cgttccatat tttcgcaaaa
    12541 cgcaaggaca tcgccgtcgg caacaatccg tacttcgtcg gcttcggcga tgcagttggt
    12601 ttcatacttg gcggcgtagc aggctttggg gctggtggcg acatagtcga tttgcggcgg
    12661 cgcgggcttg agcccgttgg tttcgagaca gaggaaatag ccttccgcct tgagcgtgtc
    12721 cagcagcata tcgagatgcg gctggatggt cggctctccg ccggtgatga tgatgttgcg
    12781 ggcggcataa gttttcagac agcctaagat gtcggacagg ctcatcatac caaatgtcaa
    12841 ataatcggta tcacaccagc cgcacgccag attgcatttg cccaagcgga cgaaaacggc
    12901 aggcataccc gtgtttcggc cctcaccttg caggctttca aagatttcga cgatgcggta
    12961 ctggggattt tcgggggcga cgctgatttt tttcataccg ccaacaccgc cgccgccaaa
    13021 aatgcgggca aaccttgttt gacgagtatg cctttgttgc cggacgaaaa cgcaccccac
    13081 gcggcggcaa tcaggacgaa gccgagaaac agtaccgtcg cgccgtacac ggcattatcc
    13141 ggagcggcaa actgcgccca caaaagcccg acggcaagaa agccgttgta cagcccttgg
    13201 ttgccaaaca gggtctgcac gcgtttctgt tccataaatt cgtaaggcag cttgaacatt
    13261 tccgccgctt tttcgccggg aatctgcgtc atttcaagcc aggcgatgta gaaatgttcg
    13321 gcggcgacga ggaggacgag aatggtggag aggagtttca tgggtgttcc tttcaatggg
    13381 tgtgccggta tgaaatgccg tctgaaaaaa cggggaatcc gtgcaggaga aaaaaccgct
    13441 ttacg
//
LOCUS       DAXDSD010000065        13406 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXDSD010000065 DAXDSD010000000
VERSION     DAXDSD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13406
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.065"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            2..1615
                     /locus_tag="ACFB2Q_001439"
     CDS             2..1615
                     /locus_tag="ACFB2Q_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC0918445.1"
                     /translation="MRRVRRAWGLRRFCRRRPAVFFKEGKLKDSVYRVCRLCAALCVW
                     GAVGAYAAGLPDVRDDAAALRAQRAAAEGWAGMPPEGDSAANGGSRVIDGDFLLSRPQ
                     LLEHVLRDALNGNQADLIASLADLYAKLPDYDAVLYGRARALLAKLAGRPAEAVARYR
                     ALHRDNAADERVLLDLAAAEFDDFRLKSAGLHFAEAAKLDLPAPVLENVGRFRKKTEG
                     LTGWHFSGGISPSVNKNANNAAPQYCLQGGGSRICSVTGPVRAAGLDYEAGAEKLTAL
                     ADNHYLLFRANVGGTSYYFSRKSAYDDAFGRAYLGWQYKNARQTVGVLPFYQAQLSGS
                     DGFDAKTKPPADRRLAPYMLAHGAGVQLAHSYRLSRRSQLSVSLERYRQRYREQGRAE
                     RNDGWQDGLYVSLARRLGGSATVFGGWQFARFVPKRETVGGAVNNAAYRRNAVHIGWA
                     QEWGGTGGLNSRIAASYAHRNYKGIAAFSTEAQRNREWNVSLALSHDKLSYKGIVPTL
                     NYRFGKTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1676..3550)
                     /locus_tag="ACFB2Q_001440"
     CDS             complement(1676..3550)
                     /locus_tag="ACFB2Q_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC0918446.1"
                     /translation="MPYFALFDDAVSRRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            3721..4650
                     /gene="ppk2"
                     /locus_tag="ACFB2Q_001441"
     CDS             3721..4650
                     /gene="ppk2"
                     /locus_tag="ACFB2Q_001441"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HFC0918447.1"
                     /translation="MTDRQLQPFENVELGEKQDQLQVFEKAVLEHEGKGSAEDSGTAP
                     LPENYPCRKRMRRAVYEAEKAKLQIELLKVQSRVKDSGQRIVSLFEGRDAAGKGGTIK
                     RFMEHLNPRGARVVALEKPTTTERGQWYFQRYIQNLPTAGEMVFFDRSWYNRAGVERV
                     TGFCEPNEYMLFMCQAPELERMLVASGIHLFKFWFSVSREEQLRRFISRRDDALKHWK
                     LSPVDIQSLDRWDDYTEAKNAMFFHTHTGDAPWVIIRSDDKKRARLNCIRYFLHQLDY
                     PGKDVKAIGKVDDKIVLVPDTRYKEKTIDVGHD"
     gene            complement(4732..6163)
                     /gene="aldA"
                     /locus_tag="ACFB2Q_001442"
                     /pseudo
     CDS             complement(4732..6163)
                     /gene="aldA"
                     /locus_tag="ACFB2Q_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(6271..7186)
                     /locus_tag="ACFB2Q_001443"
                     /pseudo
     CDS             complement(6271..7186)
                     /locus_tag="ACFB2Q_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7359..8159
                     /locus_tag="ACFB2Q_001444"
     CDS             7359..8159
                     /locus_tag="ACFB2Q_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC0918448.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            8207..8983
                     /gene="mlaE"
                     /locus_tag="ACFB2Q_001445"
     CDS             8207..8983
                     /gene="mlaE"
                     /locus_tag="ACFB2Q_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC0918449.1"
                     /translation="MNFIRSVGAKTLGLIQSFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            9034..9528
                     /gene="mlaD"
                     /locus_tag="ACFB2Q_001446"
     CDS             9034..9528
                     /gene="mlaD"
                     /locus_tag="ACFB2Q_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC0918450.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            9559..10149
                     /locus_tag="ACFB2Q_001447"
     CDS             9559..10149
                     /locus_tag="ACFB2Q_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC0918451.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGTSLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            10200..10478
                     /locus_tag="ACFB2Q_001448"
     CDS             10200..10478
                     /locus_tag="ACFB2Q_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC0918452.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            10475..11302
                     /locus_tag="ACFB2Q_001449"
     CDS             10475..11302
                     /locus_tag="ACFB2Q_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC0918453.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            11427..11789
                     /locus_tag="ACFB2Q_001450"
     CDS             11427..11789
                     /locus_tag="ACFB2Q_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC0918454.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            11789..12172
                     /locus_tag="ACFB2Q_001451"
     CDS             11789..12172
                     /locus_tag="ACFB2Q_001451"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC0918455.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGMRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            12169..12657
                     /locus_tag="ACFB2Q_001452"
     CDS             12169..12657
                     /locus_tag="ACFB2Q_001452"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC0918456.1"
                     /translation="MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(12891..13019)
                     /locus_tag="ACFB2Q_001453"
     CDS             complement(12891..13019)
                     /locus_tag="ACFB2Q_001453"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC0918457.1"
                     /translation="MAGVTVGRGSVVGAGAVITKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(13025..13387)
                     /locus_tag="ACFB2Q_001454"
     CDS             complement(13025..13387)
                     /locus_tag="ACFB2Q_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC0918458.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIGIIGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLLYSTNHKFDREKNALRA
                     TRKSVRLRWRTMSGRGAG"
BASE COUNT         2844 a         3765 c         3848 g         2949 t
ORIGIN      
        1 gatgcgccgc gtccgccgcg cgtggggctt gcggcgtttc tgccgccgcc gtccggcggt
       61 gttttttaag gaaggcaaat tgaaggatag tgtgtatcgg gtatgccggc tgtgtgccgc
      121 gctgtgtgtt tggggtgcgg tcggcgcgta tgccgccggc ttgcccgacg ttcgggacga
      181 tgcggcggcg ttgcgcgccc agcgtgcggc tgcggagggt tgggcgggaa tgccgccgga
      241 aggggattct gccgcaaacg gcggttcgcg cgtaatcgac ggggatttcc tgctgtcgcg
      301 cccgcagttg ttggaacacg ttttgcgcga cgcgctcaac ggcaatcagg cggatttgat
      361 cgcttcgttg gcggatttgt atgccaagct gccggattat gatgcggtgt tgtacggcag
      421 ggcgcgggct ttgctggcga aattggcggg aaggccggcg gaggcggtgg cgcgctatcg
      481 ggcgttgcac agggataatg cggcagacga gcgggttttg ctggatttgg cggcggcgga
      541 gtttgacgat ttccggctga agtcggcagg gctgcatttt gcggaggcgg caaaattgga
      601 tttgccggcg ccggttttgg aaaatgtggg gcgtttccgg aaaaaaacgg aggggctgac
      661 gggctggcat ttttcgggcg gcatcagccc ttctgtgaac aaaaacgcca acaatgccgc
      721 gccgcaatac tgcctgcagg gcggcggcag ccggatatgc agcgttaccg gacctgtccg
      781 tgcggcaggg ttggattatg aggccggggc ggaaaagctg actgcgctgg cggacaacca
      841 ttatctgctg ttccgcgcca atgtcggggg cacaagctat tatttcagcc gcaaatcggc
      901 ttatgacgat gctttcggca gggcttattt gggctggcag tataaaaatg cgcggcagac
      961 ggtgggggtt ttgccgtttt atcaggcgca attgtcgggt agcgacggct ttgatgcgaa
     1021 aacgaaaccg cctgccgacc gccggcttgc gccgtatatg ctggcgcacg gggcgggtgt
     1081 gcagttggcg cattcttacc gcctgagccg ccggtcgcag ctttcggtgt cgctggaacg
     1141 ttaccgccaa cgctaccgcg aacaaggccg ggcggaacgc aacgacggtt ggcaggacgg
     1201 gctttatgtg tcgctggcaa ggcggttggg cggctcggca accgtgttcg gcggctggca
     1261 gtttgcgcgg tttgtgccga aacgcgaaac ggtgggcggc gcggtcaaca atgccgccta
     1321 ccgccgcaac gccgtccata tcggttgggc gcaggagtgg ggcgggacgg gcggtttgaa
     1381 cagccggatt gccgcgtctt acgcccaccg caactacaaa ggcattgcgg cattttcgac
     1441 ggaggcgcag cgtaaccgcg aatggaatgt ctcgctggct ttgagccacg acaagttgtc
     1501 gtacaaaggc atcgtgccga cgttgaacta ccgcttcggt aagacggaaa gcaatgtgcc
     1561 gtatgcaaaa cgccgcaaca gcgaggtgtt tgtgtcggcg gattggcggt tttgaatggt
     1621 gggataatgc cgtccgaact ttgcggcagc aggttcggac ggcatttttg cgcgttcagg
     1681 caaggacggc ggcaaatacg ccgcgcaagg cgttggagag gcggatttct tcggctcctt
     1741 gcagtgtttt ttgtgtgatg tgtgtttcaa tcacttgatt tgtgtgcaaa tatttttgcg
     1801 gttcgtccga cacggcttgg cgcattatgc cgtttaaaat gtctaaatct aaagagggcg
     1861 tgagccattg tccgcgatgt ttgacgaaca cgttgctcct gccgccttcg agtaggatgc
     1921 cgtccgaatt gaaaaacagg ctgtcgaacg cgccttgcgt ttcggcggtt tgccacgctt
     1981 ggtcgaagat ggcgcggtgt gtggttttga agcggcgcag gtagttttgt gcgggcagga
     2041 tggtcggcga aatgatgacg cgctgtttgt cggtcagacg gtttaaaacg gcgtgggaca
     2101 ggctgatgcc gtctgaagcg agcagggctt tgatgcggaa cgcgccgtcg ggcaagtcgg
     2161 caatgtattg tttgatttga ttttcgcagc cgtcgggcag gggcaggttg agggcttggg
     2221 cggaggtttt cagacggcat aggtggcggt cgagcagggc gcattgcctg ttttccgcgc
     2281 gcagggtttc aaaaatgccg aaatcggggc gcagttcgtt gaggaatcgg gctttccagc
     2341 cgcattcgcg atattcggcg gcggggtcgc tgtcgatgac gatgccggaa ccgacaccgt
     2401 acacgccccg atacggcgga ttggcttcaa acggatgcgg cgtttccccg ccttgtcctg
     2461 ccgttcgggc ttgcgcgttt gaatccgtgc cgccgatgcc tgaaacaatg ccgtctgaaa
     2521 cgggtttgag cgacagggtg cggatgacga cgttgaacgc gccttcaaac cccaagccgc
     2581 cggaacacgg gttcaaatag ccgatgctgc ccgtataaag cccgcgcgct tcggtttcga
     2641 gcgattcgat aatctgcata ctcatttttt tgggcgcgcc ggtgatgctg ccgcagggga
     2701 aggcggcgcg gagggtgtcg gcgaacgagg tgtccggcag ggcttgcgcc cggatggtgc
     2761 tggtcatctg ccagacgctg ccgaaacgcg atactttaaa cggctcgggt acgcatactt
     2821 taccggtttg ggcgattttg ccgaggtcgt tgcgcagcaa atcgacaatc atcacgtttt
     2881 cggcgcggtt tttcgggtcg gtttgtaact cggcggcgcg gcgttcgtct tgtccgtcgc
     2941 ccaaaatcgg cgcggtgcct ttcatcggtt cggtgctgat ggtgccgtct gaagcgatgt
     3001 tgaggaagag ttcgggcgag aaacacagcg tccacgcgga ttgcccggct tcatcgggca
     3061 ggtgggacaa tacggcatag gggacgggct ggcgcaggcg gcggtagagg ctgacggggt
     3121 tgccgtaggc ttgcaggtgc aggcgggtgg tgtagttgat ttgataggtg tcgccgcgcc
     3181 ggatggcttc gtggatttgg cggatgcggt cgaggtaatc ggtttcggat acggaggatt
     3241 gcggcgtgga aataccggcg gggatgccgt ctgaatgtcg ggcaagccag ctttcggcat
     3301 cggtgtcggc gcagtcggca aaccagtgca gggcaatgtt gccgccgcgt tcggattcca
     3361 tccccatcag cggcaaaccg aattcgtagt ctgcaaacaa caccgaatgc agcccttttt
     3421 gccatcccga ttgcagcgcg ccgtctaaag catcgagttc ttcgggacgg aaaaaacggc
     3481 tttccacatg attttgatag agttttgcgc gtcggctgac ggcatcgtca aacagggcga
     3541 aataaggcat ggcaattcgg ggcaaatgtt ttgattatac gcccctttta cacggatttt
     3601 cagacggcgg ggcaaattcg ggcaacaaaa tgtaatttta tgtagagaaa gcggtggcga
     3661 aagtgtaaaa ttgtgaaaac ggactttcat ttccaacctg aaatacaata ggagaccttt
     3721 atgacagacc gccagttgca accgtttgaa aacgtagaat tgggcgaaaa gcaagaccag
     3781 ctccaagtat ttgaaaaagc tgttttggaa catgaaggca aaggttctgc cgaagattcc
     3841 ggcacagccc cgctgcccga aaactacccc tgccgcaaac gtatgcgccg tgccgtatac
     3901 gaagccgaaa aagccaaact gcaaatcgag ctgctgaaag tgcaaagccg ggtcaaagac
     3961 tccggccagc gcatcgtcag cctgtttgaa ggccgggatg cggcaggtaa gggcggcacc
     4021 atcaaacgct ttatggaaca tttaaacccg cgtggcgcgc gcgtggttgc tttggagaaa
     4081 ccaaccacta ccgaacgcgg acaatggtat ttccaacgct acatccaaaa cctgccgact
     4141 gcgggcgaaa tggtattctt cgaccgctcg tggtacaacc gcgccggcgt ggaacgcgtg
     4201 acgggcttct gcgaacccaa cgaatatatg ctctttatgt gccaagcccc tgagttggaa
     4261 cgtatgcttg tcgccagcgg catccacctg ttcaaattct ggttctccgt atcccgcgaa
     4321 gaacaactgc gccgcttcat ctcccgccgc gacgacgccc tgaaacactg gaaactttcc
     4381 cccgtggaca tccagtcgct cgaccgctgg gacgactaca ccgaagccaa aaacgcgatg
     4441 ttcttccaca cccacaccgg cgacgcgcct tgggtcatca tccgttcgga cgacaaaaaa
     4501 cgcgcccgtt tgaactgtat ccgctacttc ctgcaccagt tggactaccc gggtaaagac
     4561 gtgaaagcca tcggcaaagt ggacgataaa atcgttcttg ttcccgatac gcgttacaaa
     4621 gagaaaacca tcgatgtcgg tcatgactga ttgccggtga agtaataaaa atgccgtctg
     4681 aacccgattt caggttcaga cggcattttc tatccgtgtg tcaaaccggc atcagatgtc
     4741 ggtttccaga taaatgactt gggtttgcag gtattcttcc aaaccgtgtt tgccgtccgc
     4801 gccgccgatg ccggattttt tccagcccgc gtggaagccc tgcatggctt cgaagttttc
     4861 gcggttgatg taggtttcgc cgaattgcag gcggcgggta acgtagaagg cttcgttcag
     4921 gttggtggtg tacacggagc tggtcagacc gaattcgcag tcgtttgcca gggcgatgac
     4981 ctggtcgagc gtgtcgaaag cggaaacggg cagcacgagg ccgaaagttt cttctttcat
     5041 aatgtccata ctgttgtcgg tgtcggtcag cagggtaggc tcgaagaaat agccgcgtcc
     5101 ttcggtgcgt ttgccgccgc aaaccaattt cgcaccttgt ttgacggccc gttccacttt
     5161 ttcggcaacg gctttgacgg cgcgctcttc aatcagcgtg cccatttccg gcgcgcctgc
     5221 ttcggcttcg gcagggttgc cgtagcgcac gcctttcatc gcggcggtca ttttttcaat
     5281 gaacgcgtct ttcaggctgc tgtggacata gacgcgctcg gcgcagttgc agatttgacc
     5341 ggtgttgccg acgcgcgaag ccaagatgga tttcaccgct aaatccaaat ccgcgtcttt
     5401 caacacgatg gcgggggctt tgccgccgag ttccagcgag actttggtga tgttggcgga
     5461 agcggcttcc atcacttggc gacccgcttc aaccgagccg gtcaggctga ccatatcgac
     5521 ttgcggatgg gcggacaagg cgttgccgat ttccgcaccc ggaccgtcca ccacgttgaa
     5581 cacgcctgcg ggcagtccga ccgcatcgat gatttcggcg aagatgtggc agttgatcgg
     5641 ggttacgctg ctgggtttga cgacgatggt gttgcccgta accagatccg ggcccatttt
     5701 gcgggcgatc aggaagaagg ggaagttccg cggcaaaatg ccggcaatca cgcccagcgg
     5761 acgtttgaac gataaaatgt tttcgcgcgg gcggtcgctt tggatgattt cgccttcgta
     5821 acggcgcgcc cattcggctt ggtaatcgag atagtcggca gtgaacatca cttccacacg
     5881 cgccaagtct ttggttttgc cgccttcggc aacgatggtg tcggtcagct cgtcggcgcg
     5941 tccgcgtatg ccttgggcga ttttacgcaa atacgcgccg cgttcgaccg caggcagacg
     6001 ctcccaagcc ggttgcgccg cgcgcgccgc ggtcaacgtc cgccctgccg cctttgggtt
     6061 cgcgggcgat ggtttcttcg gtggacgggt tcaatacgtc gcgccattcg ccgttgaaat
     6121 cgttttcaaa gcgtccgttg atgtacatgg ccaattgttt catttcgggc cctccagttt
     6181 tgtagtcgga tgtagttttg gtttattccc aaataaattg ccgtacaagt ttctttacac
     6241 gcggattttg ggtttcaagt caaatacggc ttaccggttt tcttttttag tttccgcagg
     6301 cgtgctgccg aaatattgcc tgaatgcctg aataaagctg gaaacgtggc gctagccgca
     6361 caaatacgcg gtttcgccca cgtttttgcc cccgttttgc aacagataga gcgcgtgctg
     6421 catttgtttg tggtgcagcc actcgcttgc cgtaatgccg aaatggtcgc gcatacggcg
     6481 ttgcagcgtc ctttcgctga tgttcagcgc ggctgtcagc cggttgactt ggtgtgcgcc
     6541 gccgtcgaac gcggcattca gggtgcggct gaagtcttca gacggcatag cgtctgcttc
     6601 cgccgtttgc cccgccgccg gctcgatgcc gtctgaaacc gtgtcccaca aatccgacag
     6661 cagccgcaac acgtccgcct cgcggcgcaa tgtttcgccc aaatgcccct ttgggacggt
     6721 ttgcaggcag gatgccgcca agccgcgcag gtttgggggc aaatcccata tcctgaccgg
     6781 ttcgcggtaa agcaggggtg cgaaacgcgc gtattccgga cgcggcagcc attgttccat
     6841 acctttaatg gtcattttga ccgttttgcc gccgcgataa agatagcggc tgaacaggac
     6901 ttcttccccg acggcaatta ggacaatctt gccgccgtcc gcatcgatgc ggaagcggcg
     6961 gcggttgatg ccgaagtcca aacgcccttc cagcaacagc acgaacgaca cataaggttc
     7021 ggcgaggcgg ctgctgcaaa aatcgcaccg tgccgttacc gtaccgccgt ggatggaaat
     7081 gccgttggac aaagtatcga aacggtagct gccctccaca taagccgccg ccgcttttga
     7141 ccagattgac gaaccggctg ctgttcaaat gttctgcttt ttccatagtt ttctgataaa
     7201 ggttatcatt tgaaagataa catttttcgc cacagcaaac aatccgccgc cgacaaaggc
     7261 attttcacaa cacttgccgt ccgtatgccg tctgaaaatt gcccgtcttc ccgataagcc
     7321 ttaaaataca cgccgtcaaa cctaccgtcc gcccgtacat gagcccatcc ccctttatcg
     7381 aaatgaaaga cgtcgccttc gcgtatggcg accgcccgat tctgaacgac atcaatttca
     7441 gcattccgca aggcaatttt gccgccgtga tgggcggttc gggcagcggc aaaaccacgc
     7501 tgatgaggct gattacagga cagattcgtc cgcagtccgg gcaggttttg attgaaggac
     7561 gggatttggc gggtttttcg gctgacgaac tctacgaaca ccgccgccgt atgggcgtat
     7621 tgttccaaca cggcgcgctg tttaccgatt tgtcggtatt cgacaatatc gcttttccga
     7681 tgcgcgaact gacgcaactt ccggaagcgg ttattcgcga tttggttttg ttgaaattga
     7741 acgcggtcgg tctgcgcggc gtggaaaacc tgatgccgtc cgagttgtcc ggcgggatgt
     7801 cgcgccgcgt cgcgctggca cgcacaatcg cgctcgaccc cgaaattatg ttgtacgacg
     7861 aaccgtttac cggcctcgac ccgatttcct tgggcgtgat tgcccacttg atcagccgcg
     7921 tcaacaaggc cttgcgttcg accagcatta tggtaacgca cgacattgaa aaatctttgg
     7981 aaatcgtcga tcaggtaatt ttcttggcgc acggcgaaat tatgttctcc ggctcgccgc
     8041 aggaaatgcg cgaactggat tcgccttggg tgcgccagtt tgtcggcggg ctggcagacg
     8101 gccccgtggc ataccgctat ccggcgcaaa cgtcgttgca gcaggatttg ctcgggtaaa
     8161 tgagcaggtg ccgtctgaaa ccgttgaacc ttacatatag aaatttatga attttatccg
     8221 ttccgtcggg gcgaaaaccc tcggccttat tcaatccttc ggcagtatca cgctgtttct
     8281 gctgaacatt ttggcgaaat ccggcacggc tttcgcccgt ccgcgcctga gcgtgcgcca
     8341 agtgtatttt gccggcgtgc tgtcggtgct gattgttgcc gtttcggggc tgttcgtcgg
     8401 tatggttttg ggtttgcagg gctatacgca gttgtcgaaa ttcaaatccg ccgatatttt
     8461 gggctatatg gtcgcggctt ctctgttgcg cgaactgggt cccgtgttgg cggcgattct
     8521 gtttgccagc agcgcgggcg gtgcgatgac cagcgaaatc ggtttgatga aaacgaccgg
     8581 acagctcgaa gcgatgaacg tgatggcggt caaccccgtc gcccgcgtgg ttgccccgcg
     8641 tttttgggcg ggcgtgtttt ctatgccgct tttggcttcg attttcaacg tcgcgggcat
     8701 tttcggcgcg tatttggtcg gcgtgagctg gctgggtttg gacagcggta ttttctggcc
     8761 gcagatgcag aacaacatta cgatacatta cgatgtaatc aacggtttga tcaaatccgc
     8821 cgcgttcggc gtggcggtaa cgctgattgc cgtgcatcag ggcttccact gcatcccgac
     8881 ttcggaaggc attttgcgcg ccagcacgcg cacggtggtt tcgtccgccc tgacgatttt
     8941 ggcggtcgat tttatattga ccgcgtggat gtttacagat tgacagactg tctgaagacg
     9001 aaacgcggaa catcggatat tcaaggaact ttaatgaaaa agaacatatt ggaattttgg
     9061 gtcggactgt tcgtcctgat cggcgcggcg gcggttgcct ttctcgcttt ccgcgtggcg
     9121 ggcggcgcgg cgttcggcgg ttcggacaaa acttacgccg tttatgccga tttcggcgac
     9181 atcggcggtt tgaaggtcaa tgcccccgtc aaatccgcag gcgtattggt cgggcgcgtc
     9241 ggcgctatcg ggcttgaccc gaaatcctat caggcgaggg tgcgccttga tttggacggc
     9301 aagtatcagt tcagcagcga cgtttccgcg caaatcctga cttcgggact tttgggcgaa
     9361 cagtacatcg ggctgcagca gggcggcgat acggaaaacc ttgctgccgg cgacaccatc
     9421 tccgtaacca gttctgcaat ggttctggaa aacctgatcg gtaaattcat gaccagcttc
     9481 gccgagaaaa acgctgaggg cggcaatgcg gaaaaagccg cagaataaac atacacatcc
     9541 attttcagga aagacatcat gaaaaaatcc tccttcatca gcgcattggg catcggtatt
     9601 ttgagcatcg gcatggcatt tgcctccccg gccgacgcag tgggacaaat ccgccaaaac
     9661 gccacacagg ttttgaccat cctcaaaagc ggcgacgcgg cttctgcacg cccaaaagcc
     9721 gaagcctatg cggttcccta tttcgatttc caacgtatga ccgcattggc ggtcggcaac
     9781 ccttggcgta ccgcgtccga cgcgcaaaaa caagcgttgg ccaaagaatt tcaaaccctg
     9841 ctgatccgca cctattccgg cacgatgctg aaattcaaaa acgcgaccgt caacgtcaaa
     9901 gacaatccca tcgtcaataa gggcggcaag gaaatcgtcg tccgtgccga agtcggcatc
     9961 cccggtcaga agcccgtcaa tatggacttc accacctacc aaagcggcgg caaataccgt
    10021 acctacaacg tcgccatcga aggcacgagc ctggttaccg tgtaccgcaa ccaattcggc
    10081 gaaatcatca aagccaaagg catcgacggg ctgattgccg agttgaaagc caaaaacggc
    10141 ggcaaataac cgacaaacca taccgtccga gggcggtatg ggtttttcga acaaacgcta
    10201 tgcatacaga actcaaaaac ggaacactgc acatcggcgg cgacatcacc gtcaaaaccc
    10261 tgaccgggga cgcatttggg cgtttccggc aacaatgccg tctgaaagaa accattgccg
    10321 tcgatttcgg aggtgtcaaa cgtgccgatt ccgcctgcgt gtcgctgctg ctcgaagtgc
    10381 tgcgcggctg caaaggcagc gtcaggctga ccggcattcc cgaatccgtg cgcgcgctgt
    10441 ccgaactgta cgaaatcaaa gactggctga aatcatgaaa aaaaccgcct acgccatcct
    10501 cctgctgatc gggttcgctt ccgcccctgc atttgcagaa acccgccccg ccgaccctta
    10561 tgaaggctac aaccgcgccg tttccaaatt caacgaccaa gccgaccgct acattttcgc
    10621 ccctgccgcg cgcggctacc gcaaagttac gccgaaaccc gtccgcgccg gcgtgtccaa
    10681 tttttttaac aacctgcgcg acgtggtcag tttcggcagc aatatcttgc gtttggacat
    10741 caaacgcgca agcgaagacc tcgtccgcgt cggcatcaat accaccttcg gtttgggcgg
    10801 gctcattgat attgccggcg cgggcggcgt tcccgacaat aaaaacactt tgggcgacac
    10861 gtttgcctcg tggggctgga aaaacagcaa ttatttcgtg ttgcccgtct taggcccgtc
    10921 caccgtccgc gacgcgctcg gcacgggcat tacctctgtt tatccgccca agaatatcgt
    10981 tttccatacc cctgccggac gctggggcac gactgccgct gccgccgtca gtacgcgcga
    11041 aggcctgctc gatttgaccg acagtctgga cgaagccgcc atcgacaaat acagctacac
    11101 gcgcgacctc tatatgaaag tccgcgcacg gcagaccggt gcaacacctg ccgaaggtac
    11161 ggaagataac atcgacatcg acgaattggt cgaaagtgcc gaaaccggcg cggcagagcc
    11221 cgccgttcac gaagattccg tatccgaaac acaggcagaa gcagcagggg aagccgaaac
    11281 gcaacctgga acacaaccct aaagccatgc cgtctgaaaa tccgtgttcg gacggcattc
    11341 ccttccgtcg gaacggacag tccgacacta cagattgggc tataatccct tttttgcgca
    11401 taccgcgcga cagcaaagga aaaaccatgt acgaagtcaa ccgcagcgta ttcgtcctca
    11461 tccctttaga acctttttgg aactggctgc aaaccctgcc gggcaaccac ctcgacggac
    11521 tgaccctcga agacatccaa gccgatgcca attcctacct tgtccgcccg tgcgaaaccg
    11581 ccgacgaagt atgggacgaa atcgaagccc gtttcgaaga cattttcgcc gccgaacttg
    11641 ccgactggtg cgaagacgaa cgcgagtggc ccgcactcga tgccgacatt ttcaacgaat
    11701 ggttcgacat ccagctttct accgtcatca ccgacctcga acacgaaccg cttgcccgcg
    11761 aagccttcca acccatcaac ctgaactgat gaagctgacc gtccgcaatt accatctcga
    11821 cggctacggt catgtcaaca atgcacgcta cctcgaattt ctcgaggaag cgcgctgggc
    11881 gttttttgaa aaacgcggac tgatgcacga gttggcaggc ctcatactga ttgtcgcccg
    11941 catcgacatc cgatacagcc gtcccgccgt cgaaggcgac gtattgcagt tttcatgccg
    12001 cctgaaaacc cccggcatgc gccgcattgt gctgacccag acgattacac tgccaaacgg
    12061 caaaaccgcc gcagaagccg acatcaccct gatgcccgtc cacgccgcca cacaacgcac
    12121 cgtcagcctg cccgccaccc ttgcccgcgc actggaagcc ttgtccgaat gaaaaaaata
    12181 ctcaccgccg ccgccgtcgc actgatcggc atcctccttg ccaccgtcct catccccgac
    12241 agtaaaaccg cgcccgcctt ctccctgccc gacctgcacg gaaaaaccgt ttccaacgcc
    12301 gacctgcaag gcaaagtcac cctgattaat ttttggtttc cctcctgtcc gggttgtgtg
    12361 agcgaaatgc ccaaagtcat caaaacggca aacgactaca aaaataaaga tttccaagtc
    12421 ctcgccgttg cccagcccat cgatccgata gaaagcgtcc gccaatacgt caaagactac
    12481 ggactgccgt ttaccgtcat ttatgatgcg gacaaagccg tcggacaggc attcggcaca
    12541 caggtttatc cgacttccgt ccttatcggc aaaaaaggcg aaattctcaa aacctacgtc
    12601 ggagaacccg atttcggcaa actctaccaa gaaatcgata ccgcgctggc acaatagcga
    12661 tgccgtctga agcgcaaaaa aagcaggaac gatgttcctg ttttttataa tggattaaca
    12721 aaaaccggta cggcgttgcc ccgccccggc tcaaagggaa cgattcccta aggcgcccaa
    12781 gcaccaagcg aaccggttcc gtaccatttg tactgcctgc ggcccgccgc cttgtcctga
    12841 tttttgttaa tccactatag attatccgcc ccgacacgtt caggcggcat tcaaccttcc
    12901 ggcagattct ttttcaccac cgcaggattg cctgccgcca aagagtaggg cggaatgtct
    12961 tttgtaatca ccgcgcctgc gcccacgacc gagccgcgtc cgacggttac gcccgccatc
    13021 acaatcaccc tgcgccccgg ccagacatcg tcctccaacg taatcggacg gatttccgtg
    13081 tagccctcaa agcgtttttt tcacggtcaa acttgtggtt ggtcgaatag agcaggcatt
    13141 ccggccccat catcacattt ttgccgacca ccagcccacg gcagatttca cagtttgccc
    13201 cgatgcccga gccgtcgccc aaaaccgtat ccggaaacac atacgccccg cgtccgatat
    13261 gcggcgaaat ccgccgcgcc agaaaaccga gcacccgtct gccgatgatg ccgatgccgc
    13321 gcatatgcga cggcggcaac accgaaccgg caaaacgcag cagcaggaaa aagaattttg
    13381 atcccatcaa acctccctca aaagaa
//
LOCUS       DAXDSD010000066        13128 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXDSD010000066 DAXDSD010000000
VERSION     DAXDSD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13128
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.066"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(169..825)
                     /locus_tag="ACFB2Q_001455"
     CDS             complement(169..825)
                     /locus_tag="ACFB2Q_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC0918459.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTARFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            890..1780
                     /locus_tag="ACFB2Q_001456"
     CDS             890..1780
                     /locus_tag="ACFB2Q_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC0918460.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            1897..3708
                     /gene="typA"
                     /locus_tag="ACFB2Q_001457"
     CDS             1897..3708
                     /gene="typA"
                     /locus_tag="ACFB2Q_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC0918461.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLRVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(4105..4195)
                     /locus_tag="ACFB2Q_001458"
     tRNA            complement(4105..4195)
                     /locus_tag="ACFB2Q_001458"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4159..4161),aa:Ser,seq:gga)
     gene            complement(4234..4320)
                     /locus_tag="ACFB2Q_001459"
     tRNA            complement(4234..4320)
                     /locus_tag="ACFB2Q_001459"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4284..4286),aa:Leu,seq:cag)
     gene            4409..6784
                     /gene="rnr"
                     /locus_tag="ACFB2Q_001460"
     CDS             4409..6784
                     /gene="rnr"
                     /locus_tag="ACFB2Q_001460"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC0918462.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDL
                     CDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDAIDADAQERSTS
                     VYFPRRMIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGNPHKAQIDTLYKLFKILQKKRLARGAVEFESVETQMIFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGESGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(7123..8586)
                     /gene="guaB"
                     /locus_tag="ACFB2Q_001461"
     CDS             complement(7123..8586)
                     /gene="guaB"
                     /locus_tag="ACFB2Q_001461"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC0918463.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTYKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(8733..11291)
                     /gene="glnD"
                     /locus_tag="ACFB2Q_001462"
     CDS             complement(8733..11291)
                     /gene="glnD"
                     /locus_tag="ACFB2Q_001462"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC0918464.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPAPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIRLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPVRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSCNRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            complement(11340..11660)
                     /locus_tag="ACFB2Q_001463"
     CDS             complement(11340..11660)
                     /locus_tag="ACFB2Q_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC0918465.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            complement(11988..12461)
                     /gene="bfr"
                     /locus_tag="ACFB2Q_001464"
     CDS             complement(11988..12461)
                     /gene="bfr"
                     /locus_tag="ACFB2Q_001464"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC0918466.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELAKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(12489..12953)
                     /gene="bfr"
                     /locus_tag="ACFB2Q_001465"
     CDS             complement(12489..12953)
                     /gene="bfr"
                     /locus_tag="ACFB2Q_001465"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC0918467.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
BASE COUNT         2973 a         3286 c         3842 g         3027 t
ORIGIN      
        1 gtactgcctg cggcccgccg ccttgtcctg atttttgtta atccgctata taacggtttt
       61 ggacggaaaa acggcctgaa gccgtttcgg gcattcagac cgtttgtgcg gtggggaaaa
      121 tgccgtcaga aaggcagaaa agggcttcag acggcatgat gtcgggtttc aggacaggag
      181 caggatggcg gctgcggcaa gcgcggcaac cgataatgcg gcggcaagcg cggctttgcc
      241 ggcaaagcgg attgaggttt tgccttcgat gtatttgaag ccggttatca tcgggcggac
      301 gaggtttttc tttttgaata tgcggtatgc ggcgacggcg gcgatgtgga ctgcggaaaa
      361 aacggcgagc agcttgaaaa agttgaggtg gattttccgt ataaggctgc ccgtatgttc
      421 ggaaaccaaa tggttgaggt agccgttggt gctgaaggtg ttttcatcgg cggcaaaaag
      481 ccccgtgccg acttgaaatg agacggcggc caaaagcgca acgaccatca gtgcgcccaa
      541 ggggttgtgt ccgggctgga tatgttcggg aatgccgttt ttcagatagc cgcgtatacc
      601 tgcccaacct cggacgaaac gggagaaacg ggcggtatcg ctgccccaaa tgccccagca
      661 gaggcggaat acgagcagga aaaggacgag cagcccgacg cgcgtgtgcc attgcagcat
      721 atcgccgccg gcttttgcgc tataccacat aaagggcagg gatgcggcaa gcagccagtg
      781 gaaaaggcgg gtggggaagt cccagacttt ggttttgttt ttcataatcg gtttccggcg
      841 gtagaatcgg tttgttttcg agccttaatt ttaaccgatt ggaggggaaa tgtttcccgt
      901 tttccatctt tcaggcgaaa gccgccgccg gatgcttcag acggcattgc gttttcccca
      961 tgttttcaaa gcccgtgcgg aagattcgca caaagggact ttcggcacgc tcgccgtagt
     1021 cggcggatcg gcagggatga gcggcgcgcc cgtattggcg gcatcggcgg caatgtatct
     1081 cggctgcggt aaggtgcggg caggtttcaa tcaggatacg ctgccttttg ccgtcattgc
     1141 cggttttccc gagattatgt tggacacggc agacggtttg accaagcgtc aaggcataaa
     1201 cgcttggacg gcgggttgcg gcttgggtac agatgcggcg gcagtggaaa cggtggcggc
     1261 agtgctggcg cggaatcgag acgaagccgt cgttttggat gcggacgcgc tgaacatatt
     1321 atcaaccgat gccgaaaccc gaaatctggc gcgcgggtgt aaaaacctga ttttaacgcc
     1381 gcaccccgcc gaagccgcgc gcctgcttgg aacgacagtt gcacaggttc aggcggatcg
     1441 gacggcggca gtaaggaaga taggggcaat tctcggtgca accgtggttt taaaggggca
     1501 caaaacattg gttgccgcgt ccgatacgga aatctatgtc aacgaaagcg gcaatgcggg
     1561 attggcaacg gcgggcagtg gcgacgtatt gggcggcatc atcggcagtc tgctcgcaca
     1621 gggcgtgccg gtttttgaag ccgcctgcgc gggcgcgtgg ctgcacggcg cggcggcgga
     1681 tgttataaag gaatcggcag gcattgcggc tgggctgtcg gcaggggaaa tcgccccggc
     1741 ggcaaggtgg ctgcgcaact ggataactga aagtatgtaa gaagatgccg tctgaaaggc
     1801 aagggcttca gacggcatct tcattttcca aatactgtcc ggtaaatcgt ggtataatac
     1861 gcagttatct cttacagttt ttgaaaaaca ttaattatga aacaaatccg caacatcgcc
     1921 atcatcgcac acgtcgacca cggcaaaacc acattggtcg accaactgct gcgccaatcc
     1981 ggcacattcc gcgccaacca gcaggttgac gagcgcgtga tggacagcaa cgaccttgaa
     2041 aaagaacgcg gcatcaccat cctcgccaaa aacaccgcca tcgattacga aggctgccac
     2101 atcaatatcg tcgacacgcc gggacacgcc gacttcggcg gcgaagtgga gcgcgttttg
     2161 gggatggtgg attgcgtcgt cttgttggtg gacgcacagg aaggtccgat gccgcaaacc
     2221 cgtttcgtga ccaaaaaagc cttggctttg gggctgaaac cgattgtcgt catcaacaaa
     2281 atcgacaaac cgtccgcccg tccgagctgg gttatcgacc agactttcga gttgttcgac
     2341 aacttgggtg cgaccgacga gcagttggat ttcccgattg tttacgcttc aggtttgagc
     2401 ggctttgcca agctggaaga aaccgacgag agcagcgata tgcgcccgct gttcgacacc
     2461 atcctaaaat acacgcctgc accgagcggc agcgcggacg agccgctgca actgcaaatt
     2521 tcccaactcg actacgacaa ctacaccggc cgcctcggta tcggtcgtat cttgaacgga
     2581 cgcatcaaac ccggtcaaac cgttgccgtg atgaaccacg agcagcaaat cgcccaaggc
     2641 cgcatcaacc agcttttggg tttcaaaggc ttggaacgcg tgccgcttga agaagccgaa
     2701 gccggcgaca tcgtgattat ttccggtatc gaagacatcg gcatcggcgt aaccatcacc
     2761 gacaaagaca accccaaagg cctgccgatg ttgagagtgg acgaaccgac gctgacgatg
     2821 gactttatgg taaacaccag cccgctcgca ggtacagaag gcaaattcgt gaccagccgc
     2881 caaatccgcg accgcctgca aaaagaattg ctgaccaacg ttgccctgcg cgtggaagac
     2941 accgccgatg ccgacgtgtt ccgcgtatcc gggcgcggcg aactgcacct gacgattttg
     3001 ctggaaaata tgcgccgcga aggctacgaa ctcgccgtcg gcaagccgcg cgtcgtgtac
     3061 cgagacatcg acggtcaaaa atgcgaacct tatgaaaacc tgactgtgga cgtacccgac
     3121 gacaaccaag gcgcggtaat ggaagaactc ggccgccgcc gtggcgaact gaccaatatg
     3181 gaaagcgacg gcaacggacg cacccgcctc gaataccata ttccagcgcg cggcttgatc
     3241 ggtttccaag gcgaattcat gaccctgacg cgcggcgtcg ggctgatgag ccacgtgttc
     3301 gacgactacg cgcccgtcaa acccgatatg cccggccgcc acaacggcgt actggtgtcc
     3361 caagagcagg gcgaggcggt tgcttacgcc ttgtggaatc ttgaagaccg cggccgtatg
     3421 ttcgtatcgc ccaacgacaa aatctacgaa ggtatgatta tcggcatcca cagccgcgac
     3481 aacgatttgg tggtcaaccc gctcaaaggc aaaaaactca ccaatatccg tgccagcggt
     3541 accgacgaag cggtgcgcct gaccacgccg atcaaactga cgctggaagg cgcggtcgag
     3601 tttatcgacg atgacgagct ggtggaaatc acgccgcaat ccatccgcct gcgcaagcgt
     3661 tacctgagcg aattggaacg ccgccgtcat tttaaaaagc tggattaatg tttaaatgat
     3721 acgcgatgcc gtctgaaaaa tttcagacgg tattttttat tcggacgggc tttgcggctt
     3781 cttgaagctg tttcagacgg cgtttttcct atccaatcaa gaaactgccg ccatttttcc
     3841 agcggcatat cgccccgtcg cgtttcggta tcgggttcgg tttcccggca gcatgtcgaa
     3901 caatatcgtt tgaagggata cgccttttga gtccttacag gcttaggaag aggataaaac
     3961 ataccgcaaa acataccata aaaaaacggt aacggatagt aatcaatggt aacgtagggt
     4021 aataggttgt ggctgatttt acgcaataaa atcaatacgt aagtaacaaa aaatcccaaa
     4081 ctgtaaaagt ttgggatttg tacttggcag agaggaaggg attcgaaccc tcgatacgct
     4141 attcacgtat acacgctttc caggcgtgcg acttaaacca ctcatccacc tctctaatgg
     4201 cggaaattat cctaatcggg ataatttgtt atttggtgcc cgggagaaga ctcgaacttc
     4261 cacacccgtg aggataccag cacctgaagc tggcgcgtct accaattccg ccacccgggc
     4321 tttgctttat ttgctgtttt gcggtacagt gtcctgccgc aaagaagtcg ctattatata
     4381 gttcataaag agaatgtcaa cagtcaaaat gaataaaaat attaaatctt taaatttacg
     4441 ggaaaaagac ccgtttttaa gtcgtgaaaa acagcgttat gaacatcctt tgcccagtcg
     4501 ggaatggata atcgaattgt tggagcgcaa aggtgtgcct tcaaaaatcg aatcgcttgc
     4561 acgcgagctg tcgattacgg aagacgagta tgtctttttt gaacgccgtc tgaaggcgat
     4621 ggcgcgggac ggtcaggttt taatcaaccg ccgaggcgca gtttgcgcgg cggacaagct
     4681 ggatttggtc aaatgccgcg tcgaggcgca taaggacggt ttcggcttcg ccgtgccgct
     4741 catgccgatg gacgaagggg atttcgtttt atacgaacgc cagatgcgtg gtgtcatgca
     4801 cggcgacacc gttaccgtcc gtcctgcggg tatcgaccgc aggggccgcc gcgaagggac
     4861 ggttctggat attgtcgaac gcgcgcaaag caaagttgtc ggccgtttct atatggatag
     4921 gggcgtggcg attttggagc cggaagacaa gcgtctgaac caaagcatcg tgttggaacc
     4981 ggacggcgtg gcgcgtttca aacccgaatc cggtcaggtt atcgtcggca aaattgaggt
     5041 ttatcccgag caaaaccggc ctgcagtggc aaaaatcatt gaagttttgg gcgattatgc
     5101 cgacagcggg atggaaatcg aaattgccgt gcgcaagcat catttgccgc accgattcag
     5161 tgaagcgtgt gccaaatccg cgaaaaaaat tcccgaccat gtacgcaaaa gcgatttgaa
     5221 aggccgcgtc gatttgtgcg accttccttt ggtaacgata gacggcgaaa cggcgcgcga
     5281 tttcgacgac gcggtgtttg ccgaaaaagt cggacgcaat taccgtctgg tcgtggcgat
     5341 tgcggatgtc agccattatg tccgccctga cgatgcgatt gatgcagatg ctcaagaacg
     5401 cagtaccagc gtgtatttcc cgcgccgtat gattccgatg ctgccggaaa acctgtccaa
     5461 cggcatctgc tcgctcaatc ccgatgtcga gcgtttgtgt atggtgtgcg atatggtcgt
     5521 tacctatgcg ggcaatatca aagaataccg cttctatccc gccgtgatgc gctctcatgc
     5581 ccgcctgacc tacaaccaag tttggaaatg gctttcagac ggcatcggga atccgcacaa
     5641 agcccaaatc gacacgcttt acaagctgtt taaaattttg cagaaaaaac gtctggcgcg
     5701 cggggcggtg gagtttgaaa gcgtcgaaac ccagatgatt ttcgacgaca acggcaaaat
     5761 cgaaaaaatc gtgcccgtcg tccgcaacga tgcccacaag ctgattgaag aatgtatgct
     5821 ggcggcgaat gtttgcgcgg cggattttct gttgaaaaac aaacatacgg ctttgttccg
     5881 caaccatttg ggccccacgc ccgaaaaact cgccaccctg cgcgagcagc tcggtctgtt
     5941 ggggcttcaa cttggcggcg gcgacaaccc gtcgccgaaa gactatgccg cgcttgccga
     6001 acaattcaaa ggcaggccgg atgccgaatt gctgcaagtc atgatgttgc gctccatgca
     6061 gcaggcggtt tacgaaccgc attgcgaagg gcatttcggt ttggcttatg aagcatacgc
     6121 ccactttacc tcgcccatcc gccgctatcc cgacctgacc gtccaccgtg ccatcaaagc
     6181 cgtattgaac cggaaaacct acacgccaaa caaaagctgg caggctttgg gcgtgcatac
     6241 ttcgttttgc gaacgccgtg ccgacgatgc tggccgcgat gtggaaaact ggctgaaaac
     6301 ttattatatg cgcgataagg tcggtgaaat atttgaaggc aaaatctccg gggtggcaaa
     6361 ttttggaata tttgtcactt tggacgatat ccatatcgac ggtctggtac atatcagcga
     6421 tttgggcgaa gattatttca acttccgccc cgaaatcatg gcaatcgaag gcgaacgcag
     6481 cggcatccgt ttcaatatgg gggacagggt tgccgtccgg gtcgcgcgtg ccgatttgga
     6541 tgatggaaaa atcgactttg tcctaattgc cggagaaagc ggcaggcggc ggaaggtcaa
     6601 attatccgca tctgccaaac cggcaggggc ggcggggaaa gggaaatcga aaaccaccgc
     6661 cgagaaaaaa acagcccgat gcggcaaagt aaggggaagg ggcgtgcctg ccgttgccga
     6721 atcggggaaa aaggcaaaga aaccggttcc gattaaggtc aaaaaacgga aaggcaaatc
     6781 ataatgctga cggggcggct tggggagggc ggggcatact ttcccgaagc ggccgcccga
     6841 ttttttgata tggcggccgg cgcatcattt ccgaccaatc gaaacgcccg tattgaaaga
     6901 ttgcgtttat ttccaccgcc gttttaaagg ccgacggtat tcggcagacg gggtgcaaac
     6961 ggcgttcaga cggcattttc attctttcgg cgtgtccgtc agaatggctt tttctgtccg
     7021 cgcaatcagc ccgtgcggct tgccttgaac ggacaaaaaa tgccgtctga aacacggaaa
     7081 ttaggtttca gacggcattt tgccttgaaa aggctgttca aatcagcggt ggtaattcgg
     7141 cgcttctttg gtaatttgaa cgtcgtgaac gtgcgattcg ctcatacctg cggaagtgat
     7201 ttccacaaat tcggcttttt cgtgcatttc ggcaatattg gcgcaaccca aataacccat
     7261 actggaacgc aaaccgccgg tcagttggtg gatgatgttc acaatcgggc ctttgtaggg
     7321 aacgcggcct tcgatgcctt cggggacgta tttgtcggtg ctgtcggttt tgtcttggaa
     7381 gtagcggtcg gcagaacctt ggctcatcgc gcccaaggaa cccataccgc gataggattt
     7441 gtacgagcgg ccttggtaga gttcgatttc gcccggggct tcttccgtgc cggcaaacat
     7501 accgccgagc atgacgctgt acgcgcctgc ggcgagggct ttggcgatgt cgccggagaa
     7561 gcggatgccg ccgtcggcaa tcagcggaac gcccgtgcct ttgagggctt cggcaacgtt
     7621 gtgaatggcg gtcagttgcg gcacacccac gcctgccacg atacgggtgg tgcaaatcga
     7681 acccggaccg ataccgactt tgacggcatc cgcgccggcg gcaaccaaat ccaaagcggc
     7741 tttggcagtg gcgatgttgc cgccgatgac ttggatgtgc ggataggttt ctttgaccca
     7801 acgcacgcgg tcgatcacgc cttggctatg accgtgggca gtatcgacga caatcacgtc
     7861 cgcgccggct tcgaccaagg ctttgacgcg ctcgtcggtg tcgccgccgg tgccgactgc
     7921 cgcaccgacg cgcagacggc cttcggagtc tttgttggca ttgggaaact cggtggtttt
     7981 taaaatatct ttaacggtaa tcaagccttt aagctcgtct ttttcgttca ggaccaaaac
     8041 gcgttcgact ttgtacgtgt gcatcagttc gcgcgcttcg tctatgcttg tgccttcggg
     8101 gacggtaacc agacgttcgc gcggggtcat aatggcggaa acgggcaaat cgacgcggtt
     8161 ttcaaaacgc aggtcgcggt tggttacgat gccgacgact ttgccgtttt caacgacggg
     8221 caggccggac attttgcgtt tgcgctgcgc gcgcatttcc aagacttcgc ggatgagcgt
     8281 tgtcggcgca acggttacgg ggtctttgac cacgccgctt tcgtggcgtt ttactttgga
     8341 aacggcgcgc gcctgcattt cgggcggcat gtttttatgg atgatgccga tgccgccttc
     8401 ctgtgccatc gaaatggcga ggcgcgcctc ggtaacggta tccatcgcgg cggaaagcag
     8461 ggggaggttg agtgtgattt cgcgggtaag cttggtttga agtttaacgt ctcgcggcag
     8521 cacggtcgaa tgtgcgggaa ccaacaaaac atcgtcgaaa gtataggctt tttctacgat
     8581 acgcataatg ctcggtcttt cggtttgtgc aagatgcacg gcattatagc acgttgccgg
     8641 cggcttgaca gtttatattg tttgcaatat cccccgcctt cagacggcat acggcgtggc
     8701 ggcgggatgc cgtttgaaaa ccggcagggt gcttatgcca actgttccag caaagcctgt
     8761 ttcaatgagg actggatttt tgggtttttc aagtcggggc tgaagacggt aaagctgtct
     8821 tcgacgcgtt cgtccaacgt ggagattttg gcgtagcgca ggctgacgtt gtgggcgaag
     8881 aagacttccg ccatgtcggc gagcaggaag gggcggttga cggcggtgat ttcgacggaa
     8941 taccggtccg gatagtcttc ttcgggggtg atggtgatgc ttggcgcaat aggcatatag
     9001 cggctgcggc ggctgatgcg gcggttgcag ctttgggttt cggcaacggt gtgtccgtgg
     9061 ataaagctgt tgagttcggc ttcgagcgcg ctttggatgt cggggtagtc ttcgggggcg
     9121 tgctgcgagg ggatttgcac gatgaaggtg tcgaggatgt agtcgtgttc ggtgataaag
     9181 gcgcgggcgg cgaggatatc gaagccgtgg cggctgaaga tgcggcagag gcgggcgaac
     9241 aggcgcgggc cgttgggcat aaagaccatc acttgaaagc tgtcgctttg gggtaggatg
     9301 cggctgcgga cgatgggggg ttcaaagtcg tgtacgaggt tggcggcgtg ccacaggatt
     9361 tcgcgggact gatggcgggc gaaataggcg gaaccgagcg cgttccatag ttttttctgc
     9421 tgtttttcgg ggacggcggc gcgggtaagt aagtcggcgg cttcctgccg gcggcggccg
     9481 aagagggcgt gcgggttgcc gtcgttgcct gcgaggcagc gtccggcggc gtggaacagg
     9541 ctttccagca ggctggcgcg ccaggcgttc cacagcttgg gattggtgcc gcgtatgtcg
     9601 gaaatggtca ggaggtagag cgcgctgagg cgttcgtggg tttggacgcg tttgcagaag
     9661 gcatcgagta cgccggggtc ttggatgtct tctttttggg cgacggcaga cataaggagg
     9721 tggttttcaa ccagccaggc gagcaggtcg ctttcttctt cggtcaggaa gtggtcggcg
     9781 gcaaattggc gcgcgtctgc gatgccttgt acggcatggt cgccgccgcg tcctttggcg
     9841 atgtcgtgga agaaggcggc aaggtagagg atgtcttgtt tttcaaagga ctgcatcagc
     9901 gcggaggcgt agggcagctc atggctgtgc atatctaggg caaggcggcg gacgttgcgg
     9961 acgacggcaa ggatgtggtc gtccacggga tagatgtgga acaagtcgtg ttgaagcagg
    10021 ccgacgattt tttcccacgc gggcagatag cggcccaaca cgccgtagag gttgagaaag
    10081 cgcagggttt gggtcagtcc gttgccgctg cggaaaaaac cggcgaagcg gcgacggttt
    10141 tcagaatttt ggtagaagct gcggttgatt ttgcgcgtcg ccccccacca tgcgcgcagg
    10201 gtttgcggtt cgagcgcagt aatgtcgttg cgctgctgca tgatttcgac gattttgaaa
    10261 atgtgttcgg gccgtctgaa aaaaatatcg gtgtgccgcg cggcgatttg gttgttgact
    10321 tggatgtagt cgtcgtcaat ccgcagggta acgcgcaccg gcgtggagga aacgcggctt
    10381 cgcagcatgg gcgtgaggat gccgcccagt tgtttgacgg ttttgaccgc acggtaaaac
    10441 acgcgcatca gttcttcgct ttgacggcgg cggttttcat cttgataacc catactttcg
    10501 gcaacttgcg gctgcaaatc gaaaagcagg cggtcttcgg cgcgcttggc gtttaaatgc
    10561 agccggatgc ggatgtgggc gaggcggcgg tagccgtgcg aaagcatacc ggcttcggca
    10621 cgtgtcaaaa tccgctgttt gagcaggtcg ggcaggttgg ccgccaagcc ttgcgccttc
    10681 gctatccaaa gcagggtgtg gatgtcgcgc agaccgcccg gacagctttt gatattcggc
    10741 tccaacaccg cccccgaacc ttgcgatttg gcgtggcggt gttccatctc caccaatttt
    10801 gcttcgataa atgccgctac attgcgttgc acgttcattt tttccgccaa ttcgtctgct
    10861 gtttggcggt tgccaaacaa aaacctagcc tctaaaaacg ctgtgtcccc cgtaatatcg
    10921 tcgcgcacgc tttcgcacag ttcgtcaacg ctgccgcttt ttacagacgg cattagtttg
    10981 caatcccaca gagtttggat aaatcgggca atctgttcct gaatgccgtc tgaaagcggg
    11041 gcaggggaga caaccgccaa atccacatcc gaacagggat acggttcgcc gcgcccgaag
    11101 ccgcctaccg ccatcaggca taacgcgctg ttttgaaaat gttctgccca caatgccgcc
    11161 agcaaggttt cgactgccgc cgtgtattct ctgaaaaata ccgacacgcg gttggctttc
    11221 aaataatgcg cttcggcggc attgcgctgc tgtttgaagg tttccagtgc tgaagacagg
    11281 ttttcaggca tttttattct ttcgattggc gggaaaaagg gaggcggatg gttcggcggt
    11341 caaataccgc tttcagacgg cacttgtcgg gtatcggcgg aattggtcat caggttcaac
    11401 cgttcctgcg gcggcagcag caactgatac gccgccacgc ccaaagccgc cgccattttt
    11461 tcgatattcg acagggcaat gttccagcgt ttgcgctcga ctgccgacac ataagtcctg
    11521 tccaaaccgc attgccgcgc caattcctct tgcgaccaac ccttgttcac gcggaaaagc
    11581 cgcatattgt atgccaatac cgcccgcaaa tcctgttcgt caggcaattc ggcaggcaga
    11641 gtcaatttgt tgcccatcat ttgtttccgg ataaatggtt aaaagttaag tatttgctgt
    11701 tacggatttt acttcacata aaagccaaaa aaaaggggtg gcggcagcca cacccaaaca
    11761 cacacacaca tcaagaggaa agaggtaaaa tcaagacgga ttgggaaaca gcaaacaaac
    11821 aatctagatt tcatatcggt attatcaaac aaaaaccgtt atcgagtatt gattcaaatc
    11881 aatttagatt ttattcgtaa accggcaaag aaaaacggca tacccgttta tacggatatg
    11941 ccgtctgatg atgcagggga ctgctgtatt tggcagtggt ttgtgtttta gtcctcttgc
    12001 gccgctgttt gcaggtagtt cggcaaaccg attttgccga tcagttcctg ctgggtttcg
    12061 agccagtcga tgtgttcttc gttagtatct ttttgttttt ccaacaaatc acggctgaca
    12121 taatcctgtt gcgcttctgc tgtggcgatg gcggcaagca gggcttcgtg tttttcctgt
    12181 tctttggtca aatcgcaggc gatgatttct tcggtagatt caccaatcaa cagcttggcc
    12241 agctcttgca aattgggcaa tccctcaagg aagaggatgc gttcaatcaa atcgtcggca
    12301 gctttcattt ctacgattga ttgtttgaag aaatgttcgc ccagttcttc aaagccccag
    12361 tttttcaaaa tacgggcgtg aaggaaatat tggttaatgg ttaccagcag caagcctaag
    12421 tttttgttca gctcgcggat aaccaaacgg tcgcctttca tacggactcc ttttattcct
    12481 aatacggatt acagttggct ttggtagtag ttgccttcgc caatcagctc gatcaggcgc
    12541 agctgctgtt ccaaccagtg tgcgtggtct tcttcggtgt ctttcagttg ggcaaccatc
    12601 aggtcgcgcg taacatagtc ttgagcctct tcgcacagtt tgatgccttt tttcaaagcg
    12661 tcgcgcactt cgtattcggt ttgcaggtcg gctttgaggc aggaaaccac gtccgtgccg
    12721 atattcagtt cggcgcgtgc cattttcggc gtaccgccca gcatcaggat gcgtcggatg
    12781 aagtcttcgg cgtgtgtggt ttcttcttcc atctcgtgat tgagacgttc aaaaagtttg
    12841 gtgtagcccc attcggagta gaggcgggag tggatgaagt attggtcgcg tgccgccagc
    12901 tcgccggaca gcaattcgtt catgtaatca acaacagctt gattgccttg cataatatct
    12961 ctcttttctt aatttgggtt tcgtgcggtt aaacggcgtt ctgtaacgct gttcgggatg
    13021 cgtgcattgt atgcaaaagc tgtcggcatg acaaattttc tatttaaaat acaaacaatt
    13081 atcaaaataa atagggcgta ttccccgatg ccttccaaat cagtatgc
//
LOCUS       DAXDSD010000067        12891 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXDSD010000067 DAXDSD010000000
VERSION     DAXDSD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12891
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.067"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1146
                     /locus_tag="ACFB2Q_001466"
     CDS             <1..1146
                     /locus_tag="ACFB2Q_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFC0918468.1"
                     /translation="RGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDAL
                     EDEDVSESVDTSDKQPQDNTELHEKVEAVSLQPRAAQPRTQAAAQADAVSTNTNSALS
                     DAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRT
                     QTQIGIDRSLSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGD
                     IGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKL
                     GDDSVKVSSMAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGKSFAY
                     KADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(1222..2424)
                     /gene="trpB"
                     /locus_tag="ACFB2Q_001467"
     CDS             complement(1222..2424)
                     /gene="trpB"
                     /locus_tag="ACFB2Q_001467"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC0918469.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(2507..3136)
                     /locus_tag="ACFB2Q_001468"
     CDS             complement(2507..3136)
                     /locus_tag="ACFB2Q_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC0918470.1"
                     /translation="MSENLLEIETHPFDPVLPPKAAVMMMGTFPPKEDKRAMQFHYPN
                     FQNDMWRVYGLVFFNDAAHFRMPSHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKA
                     AREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTG
                     ETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            3412..4191
                     /gene="rsmA"
                     /locus_tag="ACFB2Q_001469"
     CDS             3412..4191
                     /gene="rsmA"
                     /locus_tag="ACFB2Q_001469"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC0918471.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGISGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFRQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            4206..4775
                     /locus_tag="ACFB2Q_001470"
     CDS             4206..4775
                     /locus_tag="ACFB2Q_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFC0918472.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            4886..5341
                     /locus_tag="ACFB2Q_001471"
     CDS             4886..5341
                     /locus_tag="ACFB2Q_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918473.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            5383..6111
                     /locus_tag="ACFB2Q_001472"
     CDS             5383..6111
                     /locus_tag="ACFB2Q_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC0918474.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            6184..6543
                     /locus_tag="ACFB2Q_001473"
     CDS             6184..6543
                     /locus_tag="ACFB2Q_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918475.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            6548..6988
                     /locus_tag="ACFB2Q_001474"
     CDS             6548..6988
                     /locus_tag="ACFB2Q_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC0918476.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKRRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            7019..8293
                     /gene="folC"
                     /locus_tag="ACFB2Q_001475"
     CDS             7019..8293
                     /gene="folC"
                     /locus_tag="ACFB2Q_001475"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC0918477.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            8307..9347
                     /gene="ftsN"
                     /locus_tag="ACFB2Q_001476"
     CDS             8307..9347
                     /gene="ftsN"
                     /locus_tag="ACFB2Q_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC0918478.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRTAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            9340..9837
                     /locus_tag="ACFB2Q_001477"
     CDS             9340..9837
                     /locus_tag="ACFB2Q_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC0918479.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            10031..11575
                     /gene="purF"
                     /locus_tag="ACFB2Q_001478"
     CDS             10031..11575
                     /gene="purF"
                     /locus_tag="ACFB2Q_001478"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC0918480.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLNKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            11675..12166
                     /gene="greB"
                     /locus_tag="ACFB2Q_001479"
     CDS             11675..12166
                     /gene="greB"
                     /locus_tag="ACFB2Q_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC0918481.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            12225..12851
                     /locus_tag="ACFB2Q_001480"
     CDS             12225..12851
                     /locus_tag="ACFB2Q_001480"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC0918482.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFINESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         3327 a         3632 c         3289 g         2643 t
ORIGIN      
        1 cgcggacgca gatctactct gccggcaccg ccctcgccat catttgattc atcagcttac
       61 gcagcaccca gggccttgca taatccggac tggtatgaaa atgattatga agaaatcccc
      121 ttggatgcgt tggaagatga agatgtatcc gaatcggttg acacatcaga caaacagcct
      181 caagacaata cggaacttca tgaaaaagtt gaggcagtga gtttgcaacc aagagccgcg
      241 cagccgcgaa cccaagccgc cgcgcaagcc gatgcagtca gcaccaatac taactcggct
      301 ttatctgacg caatggcaag cacgcaatct atcttgttgg atacaggtgc ttcattaaca
      361 cggcacattg cacaaaaatc acgcgctgat gccgaaaaaa acagtgtttg gatgtcaaac
      421 accggttatg gccgtgatta tgcttccgca caatatcgcc ggtttagttc gaaacgcacg
      481 caaacacaaa tcggcattga ccgcagcttg tccgaaaata tgcagatagg cggagtattg
      541 acttactctg acagtcagca tacttttgat caggcgggcg gcaaaaatac ttttgtgcaa
      601 gccaaccttt atggtaagta ttatttaaat gatgcttggt atgtggccgg cgatattggt
      661 gcgggcagct tgagaagccg gttacaaacg cagcaaaaag caaactttaa ccgaacaagc
      721 atccaaaccg gccttacttt gggcaatacg ctgaaaatca atcaattcga gattgtccct
      781 agtgcgggta tccgttacag ccgcctgtca tctgcagatt acaagttggg tgacgacagt
      841 gttaaagtaa gttctatggc agtgaaaaca ctaacggccg gactggattt tgcttatcgg
      901 tttaaagtcg gcaaccttac cgtaaaaccc ttgttatctg cagcttactt tgccaattat
      961 ggcaaaggcg gcgtgaatgt gggcggtaaa tccttcgcct ataaagcaga taatcaacag
     1021 caatattcag caggcgccgc gttactgtac cgtaatgtta cattaaacgt aaatggcagt
     1081 attacaaaag gcaaacaatt ggaaaaacaa aaatccggac aaattaaaat acagattcgt
     1141 ttctaaaata ccaaattcat agcaaaataa aatgccgtct gaactcaagc ttcggacggc
     1201 atttttatca gactaacaaa gctacagctc aatgcctttg agtttcgcca cggtattgat
     1261 gtctttgtcg ccgcgacccg acaggttgac caaaatcact tggtctttac ccattttcgg
     1321 cgcgtttttc accgcccaag caacggcgtg gctggattcg agcgcgggga tgatgccctc
     1381 gaatcggcag agcaagtcaa aggcttcgag tgcttcgtca tctttggcaa cggtgtattc
     1441 gacgcgcttg atgtcgtgca gatggctgtg ttccgggccg atgccggggt aatccaagcc
     1501 tgcggaaaca gagtgcgtcc ccaagacttg gccgttttcg tcctgcatca gatagctgcg
     1561 gaagccgtgc agtacgccaa tcggtgcgcc cgaagtaatc ggcgcggcgt ggtcaggcgt
     1621 atccacgccc aaaccgcctg cttctacgcc gaccaaacgt acgttttctt cttcgatata
     1681 gggatagaac aaaccgatgg cgttcgagcc gccgccgacg caggcaacgg cgacgtcggg
     1741 ctgtctgccg atagcttcct gcatctgcgc tttggcttcg ttgccgatga cgcattggaa
     1801 atcacgcacc atttcgggat acggcgcggg gccggcggcg gtgccgatga tgtagaacgt
     1861 atcgtccact cgggcgaccc attcgcgcat cgcttcgttc atcgcgtctt tcaaagtacg
     1921 gctgccactg tcaacgccga tgacattcgc accaagcagc ttcatacgga acacattcgg
     1981 catttggcgt tgaatatcgt ccgcacccat gtacacgtcg caagtcatgc cgaaacgtgc
     2041 ggcgacggtg gcagaagcca cgccgtgctg acccgcgccg gtttcggcga tgacgcgttt
     2101 tttgcccata cggcgggcaa gcaatgcctg accgatggtg ttgttgacct tgtgcgcgcc
     2161 ggtgtggttc aagtcttcgc gcttcaacca gatttgcgcg ccgcccaaat gctcggacaa
     2221 ccgcgcggcg tggtaaacgg ggctgggcct gccgacataa tgtttcaaat cgcggcggaa
     2281 ctccgcccaa aattcaggat cgtttttcgc ttcgttgtag gcctgttcca gctctttcaa
     2341 agcgggaatc agggtttcgg aaacataaag cccgccgtgt tcgccgaaaa agcccttttc
     2401 gtcgggcgcg tggtagttct tcatcggatt cttccttatg cttcaggttt tcagacggca
     2461 tttccgtttt ttcaaacatg ccgtctgaac gggaaattgt cggcaattat aactgctttt
     2521 cacacaagcc cgccatttcg aaaaacgccc gatacgccgc tgcctttttc gccaaactca
     2581 aaggataggc gcgcgaagtc gaaggcaggc gcgtcagtgt caaatcccgt ccggcaaacg
     2641 gaaacggcac ggtttcgccc gttttcggca ttttgatgcc gccgccctga atatcgagca
     2701 ggatttccgt cgccttgccg cctgtcgtac aaatatggcg gcagtctggt attttcgcca
     2761 acaccgccgc caaatcgacg gtttcaacta cctttaaaaa cttgtcggac gcattgccgt
     2821 gttcccttgc cgccttcaac acggtcggac aggacgcaat cccccgttcg tgcaaaaacg
     2881 ccttgatttt ctccgcgtca aacgcttttt cagagtgcgc agcctccccg tgggacggca
     2941 tccggaaatg cgccgcatcg ttaaaaaaca ccagcccgta aacgcgccac atatcgtttt
     3001 ggaaattcgg ataatgaaac tgcatcgcgc gtttgtcttc cttgggcgga aacgtcccca
     3061 tcatcatgac cgcagccttc ggcggcaaca cgggatcgaa gggatgggtt tcgatttcaa
     3121 gcagattttc agacacgttc aatcctccaa caggcaaagc ggcagacagc cggtttgcac
     3181 caaaccgcca aacacggcaa gccttcaaac agcattatca cgccctgccg ttttcggtta
     3241 caatgccgac atcaaacgga tactgtaaac acatttatgt tccaacacac aggacgacac
     3301 ataaagcacc gccctatgtg tcgtcctgat ttggaagggg ttacgccccg cccaaacaga
     3361 gtctgatcct gccgccctaa agggcggggt ttcaaccgaa gaggaaacac gatgaaagaa
     3421 cacaaagccc gcaagcgttt cgggcagaat tttttgcagg acacgcggat tatcggcgat
     3481 attgtcaacg ccgtgcgccc gcaggcggat gatgtcgtga ttgaaatcgg cccgggtttg
     3541 gcggcaatta ccgaaccttt ggcgaaaaag ctgaaccgcc tgcacgttgt cgaaatcgac
     3601 cgcgacatcg tatgccgtct gaaaacgctg ccgtttgcgg ataaactggt gattcacgaa
     3661 ggcgacgtgt tgcagtttga tttcaacggc atctcaggca aaaagaaaat cgtcggcaac
     3721 ctgccgtata acatctccac gccgcttttg ttcaaactgg cggaggtggc ggacgatgtc
     3781 gccgatatgc actttatgtt gcaaaaagaa gtggtcgagc gcatggttgc cgcgccgaaa
     3841 agcaacgact acggtcgctt gggtgtgatg ctgcaatatt ttttcgatat ggagctgctg
     3901 attgacgtgc cgcccgaatc gttcgatccc gcgccgaaaa tcgattccgc agtggtgcgc
     3961 atgattccgg tgaaacaccg tatcggcaag gcggacgatt tcgagcattt cgccaaactc
     4021 gtgaaactcg ccttccgcca acgccgcaaa accatacgca acaatctgaa agagcttgcc
     4081 gacgacgatg atttgcaggc agtcggcatc agtccgcaag accgtgccga acacatcgcg
     4141 ccggagaaat atgtggcgtt gagcaattat ctggcggaca aggccgtctg aaaaggaacg
     4201 cggcgatgga aagcaacctg cagaaaaccg agcgtttgat ccgcgaaatc aaccgcctgc
     4261 acgcacaata ttcgcaggat tattttgaaa ccggcaaagt gcggaaaatc aatttgagcc
     4321 acacgctcaa aaacgttccg accgaacata ttttgtctta ccgccttaat ttgcacgaag
     4381 cagttaacga ttatttagcc tttgccgata cgcgcggcat agactttttc taccgcgtga
     4441 aaaccgcaga aagcatacat gataaaatca accgctatct ggcacgcggc acccaatatc
     4501 cgaccaacaa catcctgaac gatattttcg gcgcgcggct gatttggccg tctgaaacag
     4561 tcgccgggat tttagaaaaa cttgacggct ggaaaacaga atacggcttg aaaaactggt
     4621 atttgcgcga tgccggcggc tatatcggca tacacattta tttcaaaaac agcagcaatt
     4681 tctactatcc gtgggaattg caggtttggg atgaaaaaga tgccaaagcc aatattgaaa
     4741 accatatggc gtacaaacgg aatttcgtgc gttgaattgc cggcaagccg ccttgaaacc
     4801 gtttataact gtttataaga gaataaacca taaaatacac aaacgtattc ctgacttttc
     4861 ctttaaccgc ccgagtgaac aaactatgaa atacacagcc gcactcctga cttttctttt
     4921 aactgcctgc atgaacccga acgatgcgtt tttccaaaac cggcgttatc aaatgccgga
     4981 ggcgcaattg aacggttcaa acgccgtttt ccattacggt tattcccaaa atcccgatca
     5041 tgatttattg gtcgaccaag tccgcgtgcc ggacgcgtac agggcttcat ttgcgcggga
     5101 ttggatagcg aaggagcaag gttaccgcaa aaacgactgc gtactggaaa aagcggaaag
     5161 cgggggtgtg acgtactaca cctgcgatgc ggaaaaagga ttcaacggca cacgtttcgt
     5221 catctatgtc gtgaatatga aaaacgacaa aggctacgtc aaagtttacc ggggcatgaa
     5281 taagccgaat caggaagagt taaccaaatt ggttgccgat ttgaataagt tttaccctta
     5341 atcagccaga cggattcaga ctgcccgaaa agaatggaca ctatgatcaa attcaaaaac
     5401 gtacacaaac atttcaaaga cctgcacgtc atcaacggcg taaacttgga aatcaaaaaa
     5461 ggcgaagtgg tcgtcgtctg cgggccttcg ggcagcggca aatccacact catccgcacc
     5521 gtcaaccagc ttgaaagcat cgaaagcggc gagatttggg tggacggcgt caacgttgcc
     5581 gaccccaaaa ccgatttgaa caaaatccgc gaagaagtcg gctttgtgtt tcaaagtttc
     5641 aacctctacc cgcacctgac cgtgttggac aacatcaccc tcgcaccgat gaaggttaag
     5701 gggcaaaatg cggaacaggc ggagaaaaag gcaatggagc ttttggaacg cgtcggactg
     5761 gcacacaaaa aagacgcctt cccttcccaa ctttccggcg gccagcagca acgcgtggcg
     5821 attgcgcgcg ggctggcgat ggagccgcgc gtgatgctgt ttgacgagcc gacttccgca
     5881 ctcgaccccg aaatggtcgg cgaagtgttg aaagtgatga aagacttggc ggaaagcggc
     5941 atgacgatga tgtgcgtaac ccacgaaatg gggtttgccc gcgaagttgc cgaccgcgtg
     6001 attttcgtcg ataaagggca aatccttgaa gacgaaacgc cggaagcgtt tttcaccaac
     6061 ccgaaacacg aacgcgccaa gcagttcctg caacaagtca tgacccattg atgctatgcc
     6121 gtctgaaaac cgaacgttca gacggcatag cccgacaccg caagatgatt gagaaagcac
     6181 accatgtatt ttgttgaccg caccgccgcc gtcctcaagc cgaccgcgcg ctttttggaa
     6241 tggctcaaaa gcaccgacga aaatatgccc gacctgacca tagaacagct tcgcgccgac
     6301 tgttccgtct tcctcgttcc gcagttcgac gaaccggaag ccgtcgtttc ctacttcgac
     6361 gaacgctacc ggcagatttt tgaggcggag ctggcaggtt gggacatcga caaggacaaa
     6421 tggccgcaag atatggggct gaaggcgttt tgggaatttt tcgacattga aatccacgat
     6481 atggttttgg atatggaaga agcggatttg aacatcacgc ccgtgttcga caatatgatg
     6541 taacgccatg cgcgcctttc agacggcatt gaggccgtcc cgcatcctga acatcctgac
     6601 cgtatcactc cactcggctt ccattgccgt ctgcctgact tggttttacg gccggatgat
     6661 gtggttcggt ttggcggctt tggcggcaag ttacgcatac tcgctcagga taacgaatct
     6721 gaaacgccgc catgccataa ccgccattac catagaccgc gacggacggg cggaaatcgt
     6781 atccggcaaa gacaaaacag cggcaacact tgcgggcagc agtatggtta cgccttatgc
     6841 cttattctta caatgggaca caggcggcaa aaccgtccgc caatgcatca tgcccgacat
     6901 gacggacaaa gaatcctacc ggcggctgaa agtctgggtg ctttggcggc agccgaaaaa
     6961 aaccgccgaa accgatacat cagactgatt tcagacggca tttgaaaacg aaacactcat
     7021 gaaaacatta caagactggc tctcgcattt ggaaaccgcc cacagcggcg gtttgatcga
     7081 catgggtttg gagcgcgtaa gcgaagtaaa aaagcgcatg aacctgacac cgcaatgccc
     7141 cgtcgtcgtc gtcgcgggaa caaacggcaa aggttcggtc tgcgcctacc tgacacagat
     7201 ttacaaacag gcaggcttca aaaccggcac gctgaccagc ccgcatttat tgcattacaa
     7261 cgaacgcatc gccatcaacg ccgaacccgt ttcggacgat acgattattg cctctttcga
     7321 gcgcatcgaa gccgcgcgcg gcgaaatatc gctgacttat tttgaattca atgccttggc
     7381 ggctgtcgac attttcatac gcgaacaagt ggatgtaatg atattggaag tcggcttggg
     7441 cggacgtttg gacgcggtca acgcgtttga cggcgattgc gcggtggtta ctagtgtgga
     7501 tttggaccat caggcatttt tgggcgatac ggtcgaacag gtcggctttg aaaaggcagg
     7561 cgtgttccgc agcggcaaac ccgccatctg cggtcaaaac cccgcgcccg catcattggt
     7621 cgcacacgcc gaagccatag gcgcgaaact gctgatggta cagcgcgatt tcgagtttca
     7681 cgcgatggaa aacatccaat ggaactaccg cttccggccg cagcattcag acggtcccgc
     7741 acgcaaccgc aatgccctgc ccttccccgc attgcgcggc gcataccagc tttctaacgc
     7801 cgcctgcgcg ttgaccgtat tggaatgcct cgacgacaga ctgccggtgg acattggtgc
     7861 aatcaaacgc gggctgctgc tggttgaaaa tcccggacgc ttccaagtcc tgcccggccg
     7921 gccgctgacc gtattggatg tcggacacaa cccccacgcc gcccgcgccc tgcgccgcaa
     7981 cctaatcaac cttgcctacg cgcaaaagcg caccgccgtg ttcagtatgc tgtccgacaa
     8041 agacatagac ggcgtgtcgg aaaccgttaa agaccagttt gacgagtggt atatcgcgcc
     8101 tttggacgtg ccgcgcggta tgacggcgga tgcactgaaa gccaaattgg aacaacacca
     8161 catcgaaaac atacagacat tcgccgccgt ccgcgacgcg taccgggcgg cagcctcaaa
     8221 agcgggcgaa gacgacagaa tcgtcgtatt cggctcattt cataccgtcg ccgacgtaat
     8281 gtcggtactg tgaaaaggaa acttctatgt ccgaaaacaa acaaaacgaa gtcctgaccg
     8341 gttacgaaca gctgaaacgg cgcaaccgcc gccgcctcgt aacggcaagc tccctggttg
     8401 ccgcctcctg catcctgctg gcagccgcac tcagttccga tcctgccgac agcaatcccg
     8461 caccgcaggc cggcgaaacc ggcgcaacgg aaagccaaac ggcaaacacg gcacaaaccc
     8521 ctgccttgaa atccgccgcc gaaaacgggg aaaccgccgc cgacaaaccg caggacttgg
     8581 caggcgaaga caagccttct gccgccgaca gcgaaatcag cgagcctgaa aacgtaggcg
     8641 cgccgctggt gctgattaac gaccggctcg aagacagcaa catcaaaggt ttggaagaat
     8701 ccgagaaact gcaacaggca gaaaccgcca aaaccgaacc gaagcaggca aaacaacgcg
     8761 ctgccgaaaa agtgtcggca actgccgaca gtacggatac ggtagcggtt gaaaaaccga
     8821 aacgcactgc cgaacccaaa ccgcaaaaag cggaacgcac tgccgaagcc aagcccaaag
     8881 ccaaagaaac caaaaccgcc gaaaaagttg ccgacaaacc gaaaactgct gccgaaaaaa
     8941 ccaaaccgga tacggcaaaa tccgacagcg cggtaaaaga agcgaaaaaa gccgacaagg
     9001 ctgaaggcaa aaagacagcc gaaaaagacc gttcggacgg caaaaaacac gaaacggcgc
     9061 aaaaaaccga caaagcggac aaaaccaaaa ccgccgagaa ggaaaaatcc ggcaaggcgg
     9121 gcaaaaaagc cgccattcag gcaggttatg ccgaaaaaga acgcgccttg agcctccagc
     9181 gcaaaatgaa ggcggcgggt atcgattcga ccatcaccga aatcatgacc gacaacggca
     9241 aagtttaccg cgtcaaatca agcaactata aaaacgcaag ggatgccgaa cgcgatttga
     9301 acaaactgcg cgtgcacggc atcgccggcc aggtaacgaa tgaatagcct ccccattgcc
     9361 gacctcctcg cctccgccgt catcgccgcc tgcatcgtca tttccacgat gcgcggcgtg
     9421 attgcggaag caggttcgat ggtggcatgg gtggtttcct tcttttttgc caaactcttt
     9481 gccgcaccct tcgccgacct cgcctttgcc tcgttccaac cccgcctgtt tgcattggct
     9541 ctgtcattca tttccctgtt cgtcattgcc tgtctgatcc agaaaatgct ccgttcgctg
     9601 ctgaccggcg cagtttcggc ggtcggtctg ggctttgcca accgcatttt gggcggtgta
     9661 ttcggtgcat tgaaaggcgt tttgattgtt accctgctga tcatgcttgc ttcaaaaacc
     9721 gacctgcccg ataccgaaga atggcaacag tcctataccg taccgttttt cgtatcgctt
     9781 tccgaagcgg tgttaaacca tacggacaac gcacccgaat ccctcgacga cgactaatcc
     9841 gcatacgtgg cgtttcagac agcatacctg atttatgccg tccgaaacgc cttttctttc
     9901 cttgacacac cccgacagac aaagcgcacc tgcctgactt tacacaaata ttgtcaacaa
     9961 ttcgggcaaa aagccgccgt ttgctttata attccgtttc acccttaaac ctcgaatcgg
    10021 agaagacgat atgtgcggcg tattaggttt ggtcagtcat gaacccgtga accagcttct
    10081 gtacgacggt ttgcagatgt tgcagcacag ggggcaggat gcggcgggca ttgcaacggc
    10141 ggaaggcggt accttccata tgcacaaagg caaagggatg gtgagcgaag tgttccgcac
    10201 gcgcaatatg cgcgatttga ccggcaacgc cggcatcgcc cacgtccgtt atcccacggc
    10261 gggcaacgcc ggcagcagcg cggaggcgca gcctttctac gtcagctcgc ctttcggcat
    10321 cgttttggcg cacaacggca acctcaccaa cactgccgaa ctgtatgaaa acgtgtgtaa
    10381 caaacacctg cgccacatca acaccggctc cgattccgaa gttttgctca acgtattcgc
    10441 gcacgaattg cgccgcgaag tctctaaaaa cgccgacccg caccggctca atgccgacaa
    10501 tattttcaac gccgttgccg aagtccaccg cctggtgcgc ggtgcatacg gcgtggttgc
    10561 catgattgcg ggctacggta tgctcgcctt ccgtgatcct tacggcatcc gcccgctggc
    10621 attgggttcg caaaccgaca gcgaaggcag gaaatcctat gccgtcgcct ccgaatccgt
    10681 cgccttcaac gcgcttgcct acgatttgga acgcgatatc cggccgggcg aagcggtatt
    10741 tgtcggcttt aacggcacaa tcatcgcccg acaatgcagc gaccgcgcca aactcagccc
    10801 ctgccttttt gaatatgttt attttgcccg ccccgactcc gtgattgacg gcgtgtccgt
    10861 ttaccagtcg cgcttggata tgggcgtgtc cttggcggaa aaaatcaaac gcgagctgcc
    10921 cgtggacggc atcgacgtcg tgatgcccat tcccgatacc agccgcccca gtgcaatgga
    10981 gcttgccgtc cacctcaaca aaccctaccg cgagggtttg attaaaaacc gctatatcgg
    11041 ccgcaccttt atcatgcccg gtcagtcgac gcgcaaaaaa tccgtgcgcc aaaaactcag
    11101 cccgatggaa accgaatttg caggcaaaag cgtgttgctg gtggacgact ccatcgtgcg
    11161 cgggacgacc agccgcgaaa tcgtcgaaat ggtacgcgcg gcgggcgcgc gcaaagtcta
    11221 tatcgcctcc gccgcgcccg aagtgcgcta tcccaatgtg tacggcatcg atatgcccac
    11281 gcgcgaagag ttgattgcca acgggcgcag cgcggcggaa atcgccgccg aaatcggcgc
    11341 ggacggcatc gtgtttcaag acttgggcga tttggaagcc gtcgtcaaag cactcaaccc
    11401 gaaaatcgaa tccttcgatt catcctgttt caacggcatt tatcagaccg gcgacatcga
    11461 tgatgcctac ctcgaccgcc tgtctgccga gaaatccggc tgcggcggtc tgaaaatcca
    11521 cccgagcagg atggaacaca gcatcagcat cagcgatgcg ggcgacgaag aataaggctt
    11581 cccaaaccgc gatgccgtct gaaatccgaa cgttgggttt tcagacggca tttcccatat
    11641 cggcatcaca tttcccgttt catccgcaca cgatatgagt accgaaacca aaaactacat
    11701 tacgcccgcc ggctggcagg cattgaaaga cgagctttat caactggtca ataaagaacg
    11761 ccccgaaatc gtccaaatcg tcaactgggc ggcaggcaac ggcgaccgca gcgaaaacgg
    11821 cgactatctt tacggcaaac gccgtatgcg cgaaatcgac cgccgcatcc gtttcctgac
    11881 caaacgtttg gaggctgccg tcgtcgtcga tcccgaattg cgcgaagcga ccgaccaagt
    11941 atttttcggc gcgaccgtcg gtctgttgcg cggcgacggg cgcgagcaaa ccgtcaaaat
    12001 cgtcggcgtc gatgaaatcg ataccgcgca aaacaaaatt tcttggattt cgccgttggc
    12061 gcgttgcctg attaaagcgc gcgaaggcga cgaagtggtt ttgaacacgc cggaaggccg
    12121 tgaggaaatt gaaatccttt ctgtcgaata catcaaaatc gactgatcgg gaatgccgtc
    12181 tgaaacggca caagcgcacc gtttcccaaa taaaggaata ccgaatgagg aaaatccgca
    12241 ccaaaatctg cggcatcacc acaccggaag acgcactgta tgccgcccac gccggcgcag
    12301 acgcattggg actggttttt tacccccaaa gcccccgcgc tatcgacatc attaaagcac
    12361 aaaaaatcgc cgccgcactg ccgccgtttg tcagcgttgt cgcccttttc atcaacgaaa
    12421 gcgcgcaaaa catccgccgc atccttgccg aagtgccgat acacatcatc caattccacg
    12481 gcgacgaaga cgatgcattc tgccggcagt tcgaccgccc ctatattaaa gccattcgtg
    12541 ttcagacggc atcagacatc cgaaacgccg ccacgcgctt ccccaacgct caggcactgc
    12601 tgttcgatgc ctatcaccct tcggaatacg gcggcaccgg acaccgcttc gactggacgc
    12661 tgttggcgga atattcgggc aagccgtggg tgcttgccgg cgggctgacc cctgaaaacg
    12721 tcggcgaagc cgtccgcatc accggagcgg aagcggtcga cgtatccggc ggcgtggaag
    12781 cgtctaaagg caaaaaagac cccgccaaag tcgccgcctt tatcgcaacc gccaaccgcc
    12841 tatcccgtta aagcaacaaa aattgccgcc ggaatgactt atagtggatt a
//
LOCUS       DAXDSD010000068        12888 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXDSD010000068 DAXDSD010000000
VERSION     DAXDSD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12888
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.068"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            42..647
                     /locus_tag="ACFB2Q_001481"
     CDS             42..647
                     /locus_tag="ACFB2Q_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC0918483.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            719..1111
                     /locus_tag="ACFB2Q_001482"
     CDS             719..1111
                     /locus_tag="ACFB2Q_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC0918484.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            complement(1140..1289)
                     /locus_tag="ACFB2Q_001483"
     CDS             complement(1140..1289)
                     /locus_tag="ACFB2Q_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918485.1"
                     /translation="MPSEARIAGSDGIFNVLISNRQGQHGFDTPKQVLPINGFRLRPI
                     GQNAV"
     gene            complement(1309..1755)
                     /locus_tag="ACFB2Q_001484"
     CDS             complement(1309..1755)
                     /locus_tag="ACFB2Q_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC0918486.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(1887..2099)
                     /gene="rpsU"
                     /locus_tag="ACFB2Q_001485"
     CDS             complement(1887..2099)
                     /gene="rpsU"
                     /locus_tag="ACFB2Q_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC0918487.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(2388..4238)
                     /gene="ltgA"
                     /locus_tag="ACFB2Q_001486"
     CDS             complement(2388..4238)
                     /gene="ltgA"
                     /locus_tag="ACFB2Q_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC0918488.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            4615..5352
                     /locus_tag="ACFB2Q_001487"
     CDS             4615..5352
                     /locus_tag="ACFB2Q_001487"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC0918489.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            5354..6040
                     /locus_tag="ACFB2Q_001488"
     CDS             5354..6040
                     /locus_tag="ACFB2Q_001488"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC0918490.1"
                     /translation="MADLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            6199..7065
                     /locus_tag="ACFB2Q_001489"
     CDS             6199..7065
                     /locus_tag="ACFB2Q_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC0918491.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(7128..7697)
                     /locus_tag="ACFB2Q_001490"
     CDS             complement(7128..7697)
                     /locus_tag="ACFB2Q_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC0918492.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            7855..8715
                     /locus_tag="ACFB2Q_001491"
     CDS             7855..8715
                     /locus_tag="ACFB2Q_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC0918493.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            8851..9027
                     /locus_tag="ACFB2Q_001492"
     CDS             8851..9027
                     /locus_tag="ACFB2Q_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918494.1"
                     /translation="MNTRINFTATVGKRVSQCYVTSVISTIGVTTSDAICSGGGTNKA
                     KSQCNALLKAAGRC"
     gene            9390..9743
                     /locus_tag="ACFB2Q_001493"
     CDS             9390..9743
                     /locus_tag="ACFB2Q_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC0918495.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            9733..10599
                     /gene="atpB"
                     /locus_tag="ACFB2Q_001494"
     CDS             9733..10599
                     /gene="atpB"
                     /locus_tag="ACFB2Q_001494"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC0918496.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            10656..10892
                     /gene="atpE"
                     /locus_tag="ACFB2Q_001495"
     CDS             10656..10892
                     /gene="atpE"
                     /locus_tag="ACFB2Q_001495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC0918497.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            10962..11432
                     /locus_tag="ACFB2Q_001496"
     CDS             10962..11432
                     /locus_tag="ACFB2Q_001496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC0918498.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            11437..11970
                     /locus_tag="ACFB2Q_001497"
     CDS             11437..11970
                     /locus_tag="ACFB2Q_001497"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC0918499.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ALLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            11981..>12888
                     /gene="atpA"
                     /locus_tag="ACFB2Q_001498"
     CDS             11981..>12888
                     /gene="atpA"
                     /locus_tag="ACFB2Q_001498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC0918500.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAAR"
BASE COUNT         2912 a         3478 c         3405 g         3093 t
ORIGIN      
        1 atcagccgtt ttgcaggcat cacacaggag cgacaattat tatgatgacc ctctattccg
       61 gcattacctg ccccttcagc caccgctgcc gcttcgtttt gtacgaaaaa ggtatggatt
      121 ttgaaatcaa agatatcgat atttacaaca aacccgaaga cctcgccgtc atgaacccgt
      181 acaaccaagt ccccgtgctg gtcgagcgcg atttggtgct gcacgagtcc aacatcatca
      241 acgaatacat cgacgaacgc ttcccccatc cgcagcttat gcccggcgat cccgttatgc
      301 gcggccgggg gcggctggtg ctgtaccgta tggaaaaaga attgttcaac cacgtccaag
      361 ttttggaaaa ccccgccgcc gccaacaagg aacaggcaaa agcgcgcgaa gccatcggca
      421 acggcctgac gatgctttcc ccttcgttca gcaaaagcaa atacatcctc ggcgaagatt
      481 tttctatgat tgatgttgcc ctggccccgc tgttgtggcg gctcgaccat tacgatgtca
      541 aactgggcaa aagtgccgcg ccgctgctca aatacgccga gcgtatcttc cagcgcgaag
      601 cctttatcga agcactgaca cccgccgaaa aagccatgcg caaataagtc cgaaatgctt
      661 gcaaaacccg ccgttttgca ggcattttca cattcaggca caccacacgg aacccatcat
      721 gcccacctca accaaaccct acatcctccg cgcactttgc gaatggtgca gcgacaacag
      781 cctcacgccg cacatccttg tctgggtaaa cgagcatacg cgcgtcccca tgcagtacgt
      841 ccgcgacaac gaaattatgc tcaacatcgg cgcgaccgcc acgcaaaacc ttcgaatcga
      901 caacgattgg atcagctttt ccgcccgttt cggcggacaa gcgcacgaca tatggatacc
      961 tgtcggacac gtcctcagcc ttttcgcacg ggagaccgga gaaggtatgg ggtttgaatt
     1021 ggaggagtac cgccccgaca cgccgtctga aaacacttct gccgaaaccg cgccccggcc
     1081 cgccaaaaaa ggtttgaaat tggtcaaata aatctatgcc gtctgaacgg aatcgtgttt
     1141 cagacggcat tttgtccgat ggggcgcaaa cggaatccgt ttatcggcaa aacctgtttc
     1201 ggcgtatcga aaccgtgttg cccctgccga ttcgatatta ggacattgaa aatgccgtct
     1261 gaacctgcga tacgggcttc agacggcatt tgtccgatat tcgggcaatc aggcggtcag
     1321 tacggttttc aagattttgt tgacttcgcc catatcggct ttgccggcga gacgggtttt
     1381 caatacgacc atcactttgc ccatatccgc catacctgcc gcgccggttt cggcaacggc
     1441 tgcttcgacg gcggtgcgga tttcgccggc ggagagcatt tgcggcaggt agcggtgcag
     1501 cacgtcgatt tcggcgtttt ctttgtctgc caaatcctga cggccggctt cagtgtagat
     1561 tttcgcgctg tctttgcgct gtttgaccat tttggtcagg atggcggtga ttttggcatc
     1621 gtcggcttcg gtgcgttcgt ctacttcaaa ctgtttgacg gcggcattga tgaggcggat
     1681 ggtgccgagg gaaacttgat ctttggcgcg catcgcggtt ttcatatctt cggtaaggcg
     1741 ggttttcagg ctcatgatgt cctcgctggg atgtcggatg gaaacggcgg gtttcgatgc
     1801 cgtctgaaaa gcaaaacaca ccgcagagca ggtcttgcgg tgtgtcttca tatcacaggg
     1861 ctgacctgta atctgtactt gaacgtttag tacattttgg gcggcagctg ttggctgcgc
     1921 aggcgttttt gcaggcgttt tacggctgcc gcttttttgc gtttgcgttc ggtagtcggt
     1981 ttttcgtagg cttcgcgggc gcgcagctcg gtcagcaggc cggttttttc tacggcgcgt
     2041 ttgaaacggc gcatagcgac ttcaaagggt tcgttctctt ttacgcggat tgcaggcatt
     2101 ttatttcctt taataaattc ggttgtttca tctgcccatc atatcggtgg aaagggtagg
     2161 gcagacggtg ttgtaggttt tccgtatcgc tgtgcctttc ggcggcggga tacgcgccgt
     2221 gacggaaaca tcacctccta acgggtcggc ttgtgccgtg tcaagctgct tgaaaaagta
     2281 ggaaaaacaa ttttcggatt gtcttatatt tatggttcgc cgtcaatgcc gttcgggata
     2341 aaaatgccgt ctgaaagacc gggcgggttt cagacggcat cggcacgtca gcgtgcggga
     2401 acggtgccca tacgctgttt gagcgggatg tgcggcgcgc cgaagaggga ggcgtagtag
     2461 gcggcattgg tcatcacttt tttgacatag tcgcgcgttt cggaaaacgg gatggtttcg
     2521 gcatataccg cgccttcgag gggtgtgtcc gcctgccatc ggcgcgccct gccggggccg
     2581 gcgttatagc ctgcggtggc gaggatttcg ttgttttgca ggcggcgttt ggtgtccgcc
     2641 atataccacg tccccatacg gatattgccg tcggcggtgt agagctgggc ggcatccata
     2701 ccgattttgc cggcgatttc gcgcgcggtg gcgggcataa cctgcatcag cccctgcgcg
     2761 cctacgcggg attgcgcgcc tataacgaag cggctttcct gacgaatcag gccgtaaacc
     2821 caagccggat cgacattaac attttgcgcg tggcggatta ccgtgtcttt aaacggcgaa
     2881 atatagcgca aggtgtagtt gagtttgcgg tcggtgcgtt ccgcgctgtt gaccgccata
     2941 tcgtaaaaac cgtggtcgaa cgcggtttgc gcggcggtca gcagcttgtc ttcgtcaaag
     3001 ccgcgtgtgg caaaacgcca ttccgcctga gcctgacggc gcattttcgc atcgccggcg
     3061 gttcggctgt ttcggaacag caccagcgcg cgcttaatcg cgccgtcttc cgccatacgg
     3121 aggacactgt ttttgccggc atcgggcaca ttgttgcgcg tatcgatttt gcgccccaat
     3181 tcttcccctg ccagtacggc ataaaaattc ctgcccgtcg ctgccgcctg tttgtaaagt
     3241 ttttccgctt cttgcgtgtt gcccgttgcg gcgcggctgc gtgccagcca gtagagccag
     3301 gtcgggcttt tttgcagttt ttcgggcata tgcgagatca cggaggccag ctcgtcccaa
     3361 cgtcgggcgc gcaaggcggc gcgggcgtac cactcgattt ggtcgtcggt cagttggcgg
     3421 cggtcggcaa ccttgccgta atagtccaag gcggcaggca cattgaggct ttgcgactga
     3481 taatgcccca atacgcccca cgcgaaactg cgttgttcgg ggcttaaacc gctttccatt
     3541 tcggacagca gggcggcggc attcggcgat ttgcgtgctt ctttgccgat gacgttcaac
     3601 agggcatatt cgcgcgaacc ttgtgtaccg ccgtcaaacg ggctgcccaa tgcggcggca
     3661 aggttgcgcg cgtccgtcgt ttggcggccg gccagcagtc cgcgcacgcg cctccaggca
     3721 tcgttgccgt ccaacaagcc ggatgcggct gcctgttcca acagtttggt gcagcccgaa
     3781 ggcagtttgc ccgtattttt gaccagttcg gcggcacgcg tatagtcgtt gcggctcgaa
     3841 tcggcgtagc attcgacttc ttgggcgcgc ccttcaggtt tgagtttggc atattcctgt
     3901 gcaaacagcg tccactgtct gcgcgcgccc aaagacttca gccactcgtt gcggacattt
     3961 tccgccatcg cgctgtcgcc ggcgttttcc aaataggcgg cggctgccgc atcgttgttc
     4021 tgtttcactg catccagtgc ggacgggtag ccgccgtaat ctgccagcgt ttttccttcc
     4081 ggttcggcag ggcgggtggg aacgcttgcc gaaaggtcgg cagtttctat attgtctgcc
     4141 ggggtcttgc cggctggcag tgtgtttgtc gaagaacacg cggcaagcac cagggccgcc
     4201 agcagcggca gggaatgctt catagagggt aggtacatcg gatttcctta agaatcggaa
     4261 ccctgaacgg tcagggttgg aaaagacaaa atgccgtctg aacaggcgtt tgcccgaatt
     4321 atatgccgaa actgcaccgt ctttggaatg tttccgacat aatttatatt tttcaatcat
     4381 ttgccgtttg ggtgcgaacc gctgcctttg cccgtttcag acggcattgt ccgaaatggt
     4441 tgcccgcttc ctgctttatt gacaaaaaaa cgctttcccg ataatatcct acgaaaatta
     4501 acctgccgat ttgacacagc ttgcgggcat aacagctaaa gcgttccgac aatttcagct
     4561 ttatcttccg cgcccgttgt gtccgacatc gggctttgtt gtatgggaaa gacaatgatt
     4621 attttggaca aggtttccaa gcattaccaa acgcgcgaca aaacccgttt tgccgccgtc
     4681 gagccgacca gcctcgaaat tcaagacggc gaaatcttcg ggctgatggg ctattccggc
     4741 gcgggcaaat ccactttgtt gcgcctgatc aacctgttgg aacgccccga cacgggcaaa
     4801 gtcaacgtct gcggacaaga actgaccgcg ctcgatgccg ccgcattgcg tcaggcgcgg
     4861 cagaatatcg gcatggtgtt tcagcagttc aaccttttaa gcaaccgcac cgtcgccggc
     4921 aacgttgcct ttccactgga aatcgccgga tggccgtctg aaaaaatcaa agcgcgcgtt
     4981 gccgagtgcc tcgaaatcgt cggcttgacc gaacgcgccg gccactaccc cgcccagctt
     5041 tcaggcgggc agaaacaacg cgtcggaatc gcccgcgcgc tcgcgcccaa gccgcaagtc
     5101 atcctcgccg acgaacccac ttccgccctc gaccccgcca ccacgcgcag tgtcttggaa
     5161 tgtttggaag acatcaacaa acgcttcaac gttaccatcg tcatcgtaac ccacgaaatg
     5221 agcgtcatca gacgattgtg cgaccgcgcc gccctgttgg ataaagggaa agtggtggaa
     5281 atcgtcgaag tacgcggcaa ccaaatccac gctcaatccg aaatcgggcg cgaactgatt
     5341 cgggaggact gatatggcag acttaacatt cgaacaagcc gtttccacca tcgtcggcat
     5401 gaaagacgaa atcgtccgcg ccttgggcga aactttcgtg atggtcggct tgtccaccac
     5461 attcgccgtc atcttcggca cgctcttggg cgtgttgctc ttcgtaactt ccagccgcca
     5521 actgcattac aacaagccgg taaacttcct gctcgacaac ctcgtcaacc tcatgcgcgc
     5581 cttccccttc gtcatcctga tgattgcgat gatacccgcc acacgcgcca tcgtcggcag
     5641 caccatcggc ccggttgccg cctcgctggt gttgagcgtg tcgggattgt tttattttgc
     5701 ccgactggtg gaacaaaacc tgcgcgaagt cccaaaaggt gtgattgaag ccgccgccgc
     5761 gatgggtgcg ccgccgattg ccatcgtcct caaagtcctc ttgaacgaag cgcgcgcggg
     5821 catggtttcc agcattaccg tgcttgccat cgggcttttg tcatacagcg cggcggcagg
     5881 catgataggc ggcggcggct tgggcgacct cgccatccgc tacggctact accgctacca
     5941 aaccgaagtc atcgtcttca tcgtcgccct cctcgtgctg ctggtcatcc tgattcaaag
     6001 caccggcaac gcgttggcgc ggaaactcga caaacgttaa acccgaatgc cgtctgaacg
     6061 ccaaaaaccc cgccgctatc cgaaaaatgc tataaaatcc ccctgttcgc ggcaaatgcc
     6121 gtctgaacgc cgaatccgga cggcaggact ccctgcctgt catttttgtt tgaaaccgcc
     6181 acaacatcag gagaaaatat gaaaaccttc ttcaaaaccc tttccgccgc cgcactcgcg
     6241 ctcatcctcg cagcctgcgg cggtcaaaaa gacagcgcgc ccgcagcctc tgccgccgcc
     6301 ccttctgccg ataacggcgc ggcgaaaaaa gaaatcgtct tcggcacgac cgtcggcgac
     6361 ttcggcgata tggtcaaaga acaaatccaa gccgagctgg agaaaaaagg ctacaccgtc
     6421 aaattggtcg aatttaccga ctatgtgcgc ccgaatctgg cattggcgga gggcgagttg
     6481 gacatcaacg tcttccaaca caaaccctat cttgacgatt tcaaaaaaga acacaacctg
     6541 gacatcaccg aagccttcca agtgccgacc gcgcctttgg gactgtatcc gggcaaactg
     6601 aaatcgctgg aagaagtcaa ggacggcagc accgtatccg cgcccaacga cccgtccaac
     6661 ttcgcacgcg ccttggtgat gctgaacgaa ctgggttgga tcaaactcaa agacggcatc
     6721 aatccgctga ccgcatccaa agccgatatc gcggaaaacc tgaaaaacat caaaatcgtc
     6781 gagcttgaag ccgcacaact gccgcgcagc cgcgccgacg tggattttgc cgtcgtcaac
     6841 ggcaactacg ccataagcag cggcatgaag ctgaccgaag ccctgttcca agagccgagc
     6901 tttgcctatg tcaactggtc tgccgtcaaa accgccgaca aagacagcca atggcttaaa
     6961 gacgtaaccg aggcctataa ctccgacgcg ttcaaagcct acgcgcacaa acgcttcgag
     7021 ggctacaaat accctgccgc atggaatgaa ggcgcagcca aataaggcag tcgtataaaa
     7081 tgatgccgtc tgaaccgcat tccgtgttca gacggcattt ttgtccttta atccgccatt
     7141 ccctgccatt ccgccaaatc cggcgtatcg attccgaaca gcgacagcgt gtgtgcgata
     7201 ctgtgcgcca ctatgtcgtc cgccgtctgc ggcttgcggt acatcgcagg aacaggggga
     7261 aacaccacgc cgcccatttc cgttacccgc ttcatattgt ccaaatgggc aaggttcagc
     7321 ggcgtttcgc gcaccatcag caccagccgc cgcctttcct tcaaaaccac atccgccgca
     7381 cgcgtcaaga gattgtcgcc gaagccgtgc gcgacagagg caagcgtccg catcgaacag
     7441 ggtgcgacca gcatcccgtc cgttttaaac gtaccgctgg caatgcacgc cccgatattg
     7501 ccgatcggat ggacgaaatc agccaaggca tatacttcgt ctttcgtata atccgtttcc
     7561 gaagcgcgcg ccatctccgc gcctttcgat accacaaggt gcgtttcgac atcttgcgcg
     7621 cgcaaaagtt ccaaagcctt cacgccgtat tggaaaccgc tcgccccgct gatgccgatt
     7681 atcaaacgcc gtaccatcat ccgcctttcc cataaaaccg cctgcaacgg caaaccggct
     7741 attatagtga aaaaacagaa atccgataaa cacggataca aattgtcggc aacacccaat
     7801 atccgataaa atacccgatt taacatccta tctgaatagg cacgggaggg cggtatggca
     7861 aaagtaaaag gcggattggg gcgcggcttg gattcgctgc tcgccaacgg tgcggacaac
     7921 agcagcggcg accggctgac tgcggttgcg gttaaagata tccggcccgg ccgctatcag
     7981 gcgcgcgtcc aaatcgatga cgaagccttg caggaactgg cagattcgat taaggcgcaa
     8041 ggcgtgatac agcccgtcat cgtgcgcgaa cacggactgt cccgatacga actgattgca
     8101 ggcgaacgcc gttggcgcgc cgctcagatt gccggcctgt ccgaaatccc cgccgttatc
     8161 aaaaccatca gcgacgaaac cgcattggca atgggtttga tcgaaaacct ccagcgcgaa
     8221 aacctcaacc ccatcgaaga agcacaaggc ttgaaacgcc ttgccgacga gttcgggctg
     8281 acccacgaaa ccatcgccca agccgtcggc aaaagccgaa gcgcgatttc caacagcctg
     8341 cgccttttaa gcctgcccga atccgtgcag gaaatgcttt accaacgccg cctcgaaatg
     8401 gggcacgccc gcgcactgct gaccctgccc gtcgtcgaac agctcgaatt ggcgcaaaag
     8461 gccgtcaaaa acggctggtc ggtgcgcgaa gtcgaacgcc gcagccaggc cgcccttcaa
     8521 aacaaacgtc ccgagccaaa aaaaactgcc gccgccgaca tcggccgcct gaatgatttg
     8581 ctgactgaaa aattgggtgt caacgccgaa atcaagaccg ccaaccataa aaaaggcaaa
     8641 atcatcctgc atttcgacac gcccgaaacc ttcgaccata tcttgaagca gttgggcata
     8701 gattaccggc cttaattttg cgggatatgc cgtttgaaat atagagaata gctttccaga
     8761 ttttaagtgg gaaatataat tctattgaca ttttttctgc ttcgcgtaag aatcgttttc
     8821 ctgttttcat ttttaatttt cgaggaaatt atgaacacac gcatcaactt taccgcaact
     8881 gtgggtaagc gcgtgagcca atgctatgtt accagtgtaa tcagcacaat cggcgttacc
     8941 acttccgatg caatttgttc gggaggcgga acgaacaaag ccaaaagtca atgcaatgct
     9001 ttgcttaaag cggcaggccg ctgctaatcc tttattcgga aaaggtcgtc tgaaaatatt
     9061 ttcagacgac cttttattta ttgagcaaac tccccaaact gccgacaatc cgaccgataa
     9121 actactacaa tttcgcgttg attttttatt atttccacgt aaagcaaaca aatcacgtaa
     9181 gaacattaaa agtgtaaaga aattgcggac aaactcctta atatcctgta atcatatgta
     9241 atatgtgtta gaatagcgga gttggatttt ggggagtcgc tcgtctgtat cggcggcgag
     9301 tttcacaaag attaacatat tgcttgacgt aataaggctt ctaaattata ttagccgcgc
     9361 cccaaacctg aggcggaaac attcccttta tgaagcagat tatcatccta caatccgccg
     9421 tattgtccat atgcgccgca gttgcctttg ccgtatgggg ttttgccgga ttcctttcgg
     9481 cggtcggggg cggtttgtcc tacctgcttc ccacgtttgt tgcagtttta cttctaaaac
     9541 ttttcagggg aaaccccttc ctgcaaagca ggatgttcgt cttcggagag attttaaaag
     9601 tagtgctgtc gctgttgtcc atgcttgccg tatttgcaat atggcatcaa tcgctggtgt
     9661 tcgtcccgtt tttgatgggg ctgctcggtg tcagccatct ggttttttta gtattgttga
     9721 gagtgaaaga ttatggcagg tgaaaccatt accgctgccg actacatcaa gcaccacttg
     9781 cagagcttga ccagtttgtc ggatgttact cagggtcagg gactgaaaaa cattgctgat
     9841 ttttcgttta ttaaccttga tgccgttttt tttgccgtcc tgttgggcgt aatcggcagc
     9901 ttcctgttgt ggcgcggtgc aaaaaaagca acggcaggcg ttcccggacg tttccaggcc
     9961 gccgtggaaa tcttgttcga gtttgtggac gatatgtgta agagcatcat tcacagcgaa
    10021 aagtctagaa aagccgtcgc gccgttgggt ctgacgctgt ttgtttggat tttcctgatg
    10081 aacgcgatgg atatgctgcc ggtcgatttg ctgccgatgg tatggcaggg tattaccggc
    10141 aaccatcacg ccctgctgcg catcgtaccg actgccgatt tgaacaccgc tttggcactc
    10201 gctgtcggcg tgttgctgat ttgtatctat tacaacatca aaatcaaagg attgggcggc
    10261 tggtttcacg agttgttcag tgcgccgttc ggtgcaaaac tcgcacctgc gaactttctg
    10321 ttgaacttgg tcgagtttct ttctaaaacc gtatcccacg gtatgcggtt gttcggcaat
    10381 atgtatgcgg gcgagctggt attcctgctg atagccttgc ttggcggtgc ttgggcggct
    10441 tccggcagcg ttgaagtcat ggatccgatt ctgtttgtat tccacattat tgccggtttg
    10501 gcatgggcga ttttccatat tttggtgatt accctgcagg catttatttt catggcgttg
    10561 gcgttcgtct atatcgggca ggcgcacgat gcccactgat tttcccaagt agtttgtttt
    10621 tgtagtttaa cctttgtttc tttaaggagt ttaaaatggg tttgattgct atcgcatgtg
    10681 gtttgatcgt tgcattgggt gcattgggtg catctatcgg tatcgcaatg gtcggttcta
    10741 aatatttgga gtcttctgct cgccaacctg aactgattgg tccgctgcaa accaaactgt
    10801 tcctgattgc cggtctgatt gatgccgcat tcttgatcgg tgtcgccatt gcactactgt
    10861 tcgccttcgt caacccgttt gcaggtgcat aatcaggacg gattcgtccg ttcgtacagg
    10921 tgcagcctgt ttgattaacc ccaatacgaa ggttaagtaa cgtgaatatc aatgcaacat
    10981 tattcgctca aatcatcgtc tttttcggtt tggtatggtt taccatgaaa tttgtgtggc
    11041 cgccgattgc aaaagctttg gatgagcgtg ccgcaaaaat cgccgagggc ttggctgccg
    11101 ccgagcgtgg taaaagcgat ttcgagcagg ctgaaaaaaa ggttgcagaa cttttggcag
    11161 aagggcgtaa tcaggtttcc gaaatggttg ccaacgccga aaaacgtgcc gccaaaattg
    11221 tcgaagaagc caaagaacag gcttcttccg aggcggcgcg cattgcagct caggcaaagg
    11281 ccgatgtgga gcaggaattg ttccgcgcac gcgaatccct gcgcgatcag gttgccgtgt
    11341 tggctgtcaa aggtgccgaa tctattttgc gcagcgaagt cgatgcttcc aaacacgcaa
    11401 aactgctcga taccctgaaa caggagttgt aatcttatgg cagagtttgc aacgatcgcc
    11461 agaccttatg caaaggcatt gttcggtctg gctcaggaaa aaaaccaaat tgagtcttgg
    11521 ttgggcggac tggaaaaact tgcggcggtt gttcaggaag ggaaggtggc tttattgatt
    11581 gaccgtcctg aaacgaatgc ttcagaaaaa gcagatatcc tcatcgattt ggtcggtttg
    11641 aaagacaagg agttaaaaaa ctttgttatc gtcttggccg ggcagaaacg tttgtcgata
    11701 ttgccggaag tgtatgctca atatcaagac ttgaccttat cattcaacca tatcaaatct
    11761 gccgtcattt acagtgccta tccgttgacc gacaaacagg tcggcgagtt ggcgcaaatg
    11821 ctgaataagc gtttcgacag cgagctgaaa atctctgtcg aaatcgaacc ggagctgatt
    11881 ggcggcataa aagttgaagt gggtgatcag gttttggatt tgtctgtaca aggcaaactg
    11941 agtgctttgt acacgactat gacgaattag gagagttttc atgcagctta atcctgctga
    12001 aattagcgat ctgattaaag ccaagatcga aaatctgtct gtaaatgccg aagtgagtac
    12061 ccgtggtacg gtgatttcgg taactgacgg tatcgttcgc atccatggtt tgtcagatgc
    12121 aatgcaaggt gagatgctcg aattcccggg taacacttta ggcttggcga tgaacctgga
    12181 gcgcgactcc gtcggtgccg tagtgttggg cgagtacgaa catattaaag aaggcgacac
    12241 ggttacctgt accggccgta ttttagaagt gccggtcgga cgcgaattgg tcggacgcgt
    12301 cgtcgatgca ttgggtcggc ctatcgacgg caaaggcccg attaatacaa ctttgaccgc
    12361 ccctgtcgaa aaaatcgcac cgggcgtgat tgcacgcaaa tcggttgatc agccgatgca
    12421 aaccggtctg aaggcgattg actctatgat tcctgtcggt cgcggtcagc gtgagttgat
    12481 tattggagac cgtcagacag gtaaaacggc cgtggcattg gatgccatcg tcaaccaaaa
    12541 aggtacgggt gttatctgta tctatgtcgc tatcggtcaa aaagcatctt ctattgccaa
    12601 cgtggtccgc aaattggaag agcatggcgc gatggaacac acgattgtgg ttgctgcaac
    12661 tgcatctgaa gcggcggcat tgcaatatat cgcaccttat tcaggttgta cgatgggtga
    12721 attcttccgc gatcgtggcg aagatgcctt gattgtttat gacgatttgt ccaaacaggc
    12781 tgtggcttac cgtcaaattt ccctgctttt gcgccgtccg cccggccgcg aagcctatcc
    12841 cggcgatgtg ttctacctgc actcccgtct gttggaacgt gcggcacg
//
LOCUS       DAXDSD010000069        12539 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXDSD010000069 DAXDSD010000000
VERSION     DAXDSD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12539
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.069"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            153..1217
                     /locus_tag="ACFB2Q_001499"
     CDS             153..1217
                     /locus_tag="ACFB2Q_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC0918501.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPGANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1358..1681
                     /locus_tag="ACFB2Q_001500"
     CDS             1358..1681
                     /locus_tag="ACFB2Q_001500"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC0918502.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1755..2078
                     /locus_tag="ACFB2Q_001501"
     CDS             1755..2078
                     /locus_tag="ACFB2Q_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC0918503.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2125..2328
                     /gene="tatA"
                     /locus_tag="ACFB2Q_001502"
     CDS             2125..2328
                     /gene="tatA"
                     /locus_tag="ACFB2Q_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC0918504.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2332..3018
                     /gene="tatB"
                     /locus_tag="ACFB2Q_001503"
     CDS             2332..3018
                     /gene="tatB"
                     /locus_tag="ACFB2Q_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC0918505.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQELDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3031..3801
                     /gene="tatC"
                     /locus_tag="ACFB2Q_001504"
     CDS             3031..3801
                     /gene="tatC"
                     /locus_tag="ACFB2Q_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC0918506.1"
                     /translation="MSETQNEQTVQPLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARPYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3845..4453
                     /locus_tag="ACFB2Q_001505"
     CDS             3845..4453
                     /locus_tag="ACFB2Q_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC0918507.1"
                     /translation="MNTLYLGSGSPRRMEILTQLGYRVVKLPAGIDETVKAGETPAPY
                     VQRMAEEKNQAALTLFCETNGAMPDFPLITADTCVFSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMEKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRPPLSALKP"
     gene            4462..4815
                     /locus_tag="ACFB2Q_001506"
     CDS             4462..4815
                     /locus_tag="ACFB2Q_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC0918508.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFNAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4805..6733
                     /locus_tag="ACFB2Q_001507"
     CDS             4805..6733
                     /locus_tag="ACFB2Q_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC0918509.1"
                     /translation="MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWWLQARLMNLIYPGMNDI
                     ASWVFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWENTPL
                     LQNIIVHRGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNAILETF
                     TGIRYETAVERVANGGFTDLPRQSEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNFLSTLFTHSHNIILQLLAEMGISGTLLVAATLLTGIAGLLKRSLTPASLFLLC
                     ALAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNSFSPAADDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPETKPCKRPASADADTARNVKLHARHC
                     KKQQTGREIRYQKTLQPC"
     gene            6809..7486
                     /gene="misR"
                     /locus_tag="ACFB2Q_001508"
     CDS             6809..7486
                     /gene="misR"
                     /locus_tag="ACFB2Q_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC0918510.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7499..8905
                     /gene="misS"
                     /locus_tag="ACFB2Q_001509"
     CDS             7499..8905
                     /gene="misS"
                     /locus_tag="ACFB2Q_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC0918511.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQAIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9023..11446
                     /locus_tag="ACFB2Q_001510"
                     /pseudo
     CDS             9023..11446
                     /locus_tag="ACFB2Q_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11491..11736
                     /gene="rpsP"
                     /locus_tag="ACFB2Q_001511"
     CDS             11491..11736
                     /gene="rpsP"
                     /locus_tag="ACFB2Q_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC0918512.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11752..12261
                     /gene="rimM"
                     /locus_tag="ACFB2Q_001512"
     CDS             11752..12261
                     /gene="rimM"
                     /locus_tag="ACFB2Q_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC0918513.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12261..>12539
                     /locus_tag="ACFB2Q_001513"
     CDS             12261..>12539
                     /locus_tag="ACFB2Q_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC0918514.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKP"
BASE COUNT         3279 a         4063 c         2758 g         2439 t
ORIGIN      
        1 gagggaaggg aattttgcaa aggtctcagt ttgtagtaaa atatctaaac agattcaatc
       61 ggaaacggtc aggcttcttc acgccattcc catttcaacc gccgcctgct agattcagac
      121 ggcattccta cttgaaaaca aggagcgtta caatgaaagc agcacgtttt tacaacaaag
      181 gcgacatccg catcgaagac atccccgaac cgaccgtcgc ccccggcacc gtcggcatca
      241 atgtcgcctg gtgcggcatc tgcggtaccg acctgcacga atttatggaa ggcccgattt
      301 tcatcccgcc ctgcggtcat ccgcatccga tttccggcga gtccgcgccc gtaacgatgg
      361 gacacgagtt ctccggcgtg gtttatgccg tcggcgaagg cgtggacgac attaaagtcg
      421 gccaacacgt cgtggtcgaa ccctacatca tccgcgacga cgtaccgacc ggcgaaggca
      481 gcaactacca cctctccaaa gacatgaact ttatcggctt gggcggctgc ggcggcggcc
      541 tgtccgaaaa aatcgccgtc aaacgccgtt gggtgcatcc gatttccgac aaaatcccgt
      601 tggatcaagc tgctttgatt gaacctctgt ctgtcggaca ccacgcctat gtacgcagcg
      661 gcgcgaaagc aggcgatgtc gcattggtcg gcggcgcagg cccgatcggt ttgctgttgg
      721 ccgccgtgtt gaaagccaaa ggcatcaaag tcatcattac cgagttgagt aaagcacgca
      781 aagacaaagc ccgcgaatcc ggcgttgccg actacatcct cgacccgtcc gaagtcgatg
      841 ttgttgaaga agtgaaaaaa ctgaccaacg gcgaaggcgt ggacgtggcg ttcgaatgta
      901 ccagcgtgaa caaagtgttg gatactttgg tcgaagcctg caaacccggc gccaacttgg
      961 ttatcgtatc catctggagc caccccgcca ccgtcaacgt ccacagcgtc gtgatgaaag
     1021 aactggatgt gcgcggcacg attgcctact gcaacgacca cgcggaaacc atcaaactgg
     1081 tcgaagaagg caaaatcaac ctcgaaccct tcatcaccca gcgcatcaag ctggacaaac
     1141 tggtttccga aggcttcgag cgtctgatcc acaacaacga atccgccgtt aaaattattg
     1201 tgaatccaaa cctgtaagca aattacattt gcaacagcac aaatgccgtc tgaaacgctc
     1261 agacggcatt ttccaacgaa acgcaacagg cgtattgccc gagagcagcc gtttagccta
     1321 tcattccgaa caaacagaaa caaacagaag gaaaatcatg ggagattccg tactatccgc
     1381 catccaacaa accattatac agcgcaaatc tgccgacccg tccgaatctt acgtcgcaca
     1441 gctcctgcac aagggcgagg acaaaatcct aaaaaaagtg attgaagaag cgggcgaagt
     1501 gttgatggca tccaaagaca aagacccgtc ccacctggtt tacgaagttg ccgacttatg
     1561 gttccacacc atgattcttc tgacacacca cgacctgaag gcggaagacg tattggacga
     1621 actttcccgc cgtcaggggc tgtcggggct ggccgaaaaa gccgcccgta cagaatcctg
     1681 aatttatatt acaatctcat cccttccacg tttaatccgt ctgcgtgctt ttttcagacg
     1741 gcaacggagc cgttatggac aactgtattt tctgcaaaat cgccgccaaa gagattccgg
     1801 cgcaaaccgt ctatgaagac ggcgaaatgg tttgtttcaa agacatcaac cccgccgctc
     1861 cgcttcatct gctgctgatt cccaaagtcc attttgattc gttggcacac gccgcgcccg
     1921 aacatcagcc ccttttggga aaaatgatgc tgaaagttcc cgaaatcgcc aaagcttcgg
     1981 gactgaccga cggtttcaaa acccttatca ataccggcaa aggcggcgga caagaggtct
     2041 tccacctgca tatacacatc atgggcacac ccgtataaac cgttatttca caatcaaccc
     2101 ctaatactta cttaaggata catcatgggc agtttttctc tgacgcactg gattatcgta
     2161 ctgattatcg tcgttttgat attcggcacc aaaaaattgc gcaacgtcgg caaagacctc
     2221 ggcggtgcgg ttcatgactt caaacagggg ctgaacgaag gcacagacgg caaagaagcc
     2281 caaaaagacg atgtaatcga acacaaaaaa gacgaagaca aagcataact tatgtttgat
     2341 ttcggtttgg gcgagctgat ttttgtcggc attatcgccc tgattgtcct tggtccagaa
     2401 cgcctgcccg aagccgcccg cactgccgga cggcttatcg gcaggctgca acgctttgta
     2461 ggaagcgtca aacaagaact tgacactcaa atcgaactgg aagagctgag gaaggtcaag
     2521 caggcattcg aagctgccgc cgctcaggtt cgagacagcc tcaaagaaac cgatacggat
     2581 atgcagaaca gtctgcacga catttccgac ggtctgaagc cttgggaaaa actgcccgaa
     2641 cagcgcacgc ctgccgattt cggtgtcgat gaaaacggca atccccttcc cgatacggca
     2701 aacaccgtat cagacggcat ttccgacgtt atgccgtctg aacgttccga tacttccgcc
     2761 gaaacccttg gggacgacag gcaaaccggc agtacagccg aacctgcgga aaccgacaaa
     2821 gaccgcgcat ggcgggaata cctgactgct tctgccgccg cacctgtcgt acaggccgtc
     2881 gaagtcagct atatcgatac tgctgttgaa acgcctgttc cgcacaccac ttccctgcgc
     2941 aaacaggcaa taaaccgcaa acgcgatttt cgtccgaaac accgcgccaa accgaaattg
     3001 cgcgtccgta aatcataaag ggggcatccg gtgtccgaaa cacaaaacga acaaaccgtc
     3061 caaccgcttg tcgaacacct catcgagcta cgccgccgcc tgatgtggat ggttgtcggc
     3121 attctcgtct gctttttcgg catgatgccg tttgcccagc agctctatac ttttatcgcc
     3181 gacccgctga tggcaaacct gcccaaagac accagtatga ttgcgaccga tgtcatcgca
     3241 ccgtttttcg ttccggtcaa agttaccctg atggcggcgt ttttggtttc actgccgcat
     3301 acgctctacc aaatctgggc attcgtcgcc cccgcactct accaaaacga aaaacgcctg
     3361 attacgccgc tcgtcctctc cagcgtcagc ctgtttttca tcggcatggc atttgcctat
     3421 ttcctggttt tccccgtcat tttcaaattc cttgccggcg taacccccgt cggggtcaat
     3481 atggcgaccg acatagacaa atacctgtcc tttatcttag gtatgttcgt tgcgttcggc
     3541 acggcatttg aagtgcccat cgtcgtcatc ctgttaacca aaatcggcgc agtcacgacc
     3601 gagcagctca aacacgcccg gccttatgtc attgtcggcg cgtttgtcgt tgccgcagtc
     3661 atcacgccgc ccgacattat ttctcagacc ctgcttgcca ttccgctgat cctcttatac
     3721 gaggcgggca tttggttcgg acgcttcttc actccacgtt cagaacagga tggcgacata
     3781 cagccgcctg caaaaacctg acactatgcc gtctgaagcc ccgccgcaca ttaaggagca
     3841 caccgtgaac accctttact taggttcggg cagcccgcgc cggatggaaa tcctgacaca
     3901 gttgggctat cgggtcgtca agctgcccgc cggcatcgac gaaaccgtta aagccggcga
     3961 aacgcccgcc ccttacgtcc aaaggatggc ggaagaaaaa aatcaggcgg ctttgaccct
     4021 cttttgcgaa accaacggcg caatgcccga tttccccctg attaccgccg acacctgcgt
     4081 cttttcagac ggcatcatat tgggcaaacc tcgctcccaa gccgaagcaa tcgaattttt
     4141 aaaccgattg tccggcaaac aacataccgt cctgactgct gtctgcattc attatcgcgg
     4201 caaaacgtca agccgcgtcc aaaccaaccg cgtcgttttc aagcccctga gttcggaaga
     4261 aatttccgcc tatgtgcaaa gcggcgaacc gatggagaaa gccggtgcct acgccgtgca
     4321 aggcataggc ggtatcttta tccaatctat cgaaggcagc ttcagcggca ttatggggct
     4381 gcccgtttat gaaaccgttt ccatgttgca ggatttggga taccgccccc ccttatccgc
     4441 ccttaaaccg taaagtccgc cgtgaacaga caaaccgctt acttccttgc ctccttcagc
     4501 ctgattgcac tgattgccct atccctttct tgggaattat ggattgcacc gttacgcccg
     4561 ggcggttcgt ggctcgcgct caaagccctg cccctctgcc tgccgctttc aggtattcta
     4621 aagaaaaaaa tctataccta ccaatacagc tccatgctgg ttctgattta ctttgccgaa
     4681 gccgtcatgc gcctgttcaa cgcctatccc gcagaaaaaa tttgcgccgc gctttccgca
     4741 gtattcagca tcatcttctt catatcctgc ctgtcgttcg tcaaacaata caaggaaaca
     4801 aacaatgtcc gctgaaacga ccgtatccgg cgcgcgcccc gccgccaaac tgccgattta
     4861 catcctgccc tgcttccttt ggataggcat catccccttt accttcgcac tcaggctgaa
     4921 accgtcgccc gacttttacc acgatgccgc cgccgcggcc ggcctgattg tcctgttgtt
     4981 cctcacggca ggaaaaaagc tgtttgatgt caaaatcccc gccatcagct tcctcctgtt
     5041 tgcaatggcg gcattttggt ggcttcaggc acgcctgatg aacctgattt atcccggaat
     5101 gaacgacatc gcctcttggg ttttcatctt gctcgccgtc agcgcgtggg cctgcaagag
     5161 tttggtcgca cactacggac aagaacgcat cgttaccctg tttgcctggt cgctgcttat
     5221 cggctccctg cttcaatcct gcatcgtcgt catccagttt gccggctggg aaaacacccc
     5281 cctgcttcaa aacatcatcg ttcacagagg gcaaggcgta atcggacaca tcgggcagcg
     5341 caacaacctc ggacactacc tcatgtgggg catactcgcc tccgcctacc tcaacggaca
     5401 acgaaaaatc cccgcagccc tcggcgcaat ctgcctgatt atgcagaccg ccgttttagg
     5461 tttggtcaat tcgcgcacca tcttgaccta catagccgcc atcgccctca tccttccctt
     5521 ctggtatttc cgttcggaca aatccaacag acggacgatg ctcggcatag ccgcagccgt
     5581 attccttacc gcgctgttcc aattttccat gaacgccatt ctggaaacct ttacaggcat
     5641 ccgctacgaa actgccgtcg aacgcgtcgc caacggcggt ttcacagact tgccgcgcca
     5701 aagcgaatgg aataaagccc ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg
     5761 gaacagtttt gcccaacaaa ccttcctgat caatgccgaa cagcacacca tacacgacaa
     5821 cttcctcagc accttgttca cccattccca caacatcatc ctccaactcc ttgcagaaat
     5881 ggggatcagc ggcacgcttc tggttgccgc aaccctgctg acgggcattg ccgggctgct
     5941 gaaacgctcc ctgacccccg catcactttt cctgctgtgc gcgcttgccg tcagtatgtg
     6001 ccacagtatg ctcgaatatc ctttgtggta tgtctatttc ctcatcccct tcggactgat
     6061 gctctttctg tcccccgcag aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct
     6121 cggcatactg accgcctccg ccgccatatt cgcaggattg ctgcacttgg actggacata
     6181 cacccggctg gttaactcct tttcccccgc cgctgacgac agtgccaaaa ccctcaaccg
     6241 gaaaatcaac gaactgcgct atatttccgc aaacagcccg atgctgtcct tttatgccga
     6301 cttctccctc gtaaacttcg ccctgccgga ataccccgaa acccagactt gggcggaaga
     6361 agcaaccctc aaagcactaa aataccgccc ctactccgcc acctaccgca tcgccctcta
     6421 cttgatgcgg caaggcaaag ttgcagaagc aaaacaatgg atgcgggcaa cacagtccta
     6481 ttacccctac ctgatgcccc gatacgccga cgaaatccgc aaactgcccg tatgggcacc
     6541 gctgctgccc gaactgctca aagactgcaa agccttcgcc gccgctcccg gccatccgga
     6601 aacaaaaccc tgcaaacgac ccgcgtcggc ggatgcggat accgcccgaa atgtaaagct
     6661 ccatgcaaga cattgcaaaa aacagcaaac cggtagggaa atacgctatc aaaaaacatt
     6721 gcagccgtgt taagataaac tgtcaaacaa tcttttcacg ccacgcccga aacagggtcg
     6781 gggcataccc ttacgaaaag gaaacaccat gagccgcgta ttactcgtag atgacgatgc
     6841 cctgctgacc gaactgctga ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt
     6901 tcccgacggg gaagcaggcg tacaggaaat cctgagcggg caatacgatg tagtcgtatt
     6961 ggattccatg atgcccaaaa tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag
     7021 caccgtcccc atcatcatgc tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt
     7081 ggaaatgggt gcggacgact atgtccccaa accctgcaca ccacgcgaac tcttggcacg
     7141 catcaatgcc atcctccgcc gagcacaaca cagcggcgaa cagaacaacg cacccaacag
     7201 catctccgtc agcgatgtcg tcctataccc cgccaaacgc caggcatccg tcaaagacat
     7261 gccgctcgaa ctgaccagca ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg
     7321 acaggtagtc agcaaagaaa ccctgtccgt cgaagcactc gaccgcaaac tggcaaaatt
     7381 cgaccgcagt atcgacgtac acatctccag catccgccac aagctgggcg atgcctccct
     7441 gattcaaacc gtacgcggct tgggctacct gtttgtcaaa aactgaagca gcgggcaaat
     7501 gaaactgttc caacgcattt tcgccacatt ttgcgcggtt atcgtctgcg caatctttgt
     7561 ggcgagtttt tctttttggc tggtgcagaa cacccttgcc gaaaaccaat tcaaccaacg
     7621 ccgcaccatc gaaaccacat tgatgggcag cattatttcc gcattcaaga cacggggcga
     7681 caacggcgcg cgcgaaatcc tgaccgaatg gaaaaacagc cccgtctcat ccgccgttta
     7741 cgtcatacag ggcgacgaga aaaaagacat cttaaaccgc tatatcgaca attacaccat
     7801 agaacgcgcc cggttgtttg ccgccaacaa cccccattcc aaccttgtcc gcatcgaata
     7861 cgaccgtttc ggcgaagaat acctgttctt cattaaaggc tgggacaacc accaggcaca
     7921 acgcctgccc agcccgctgt ttatcccggg cctgccgctt gccccgattt ggcacgaatt
     7981 catcatcctc tccttcatca tcattgtcgg actgctgatg gcatatatcc ttgccggcaa
     8041 cattgccaaa cccatcagaa tcttaggcaa cggcatggac agggtggcaa acggagaact
     8101 tgaaacccgc atctcccaac aggtcgacga ccgcgacgac gaattgtccc atcttgccat
     8161 ccaattcgac aaaatggcag aaaaactcga aaaacttgtc gccaaagaac gttacctact
     8221 ccaccacgtt tcccacgaaa tgcgctcccc gcttgcccgt atgcaggcaa ttgtcggact
     8281 gattcaggca cagccccaaa aacgggagca atatctcaaa cggctggaag gcgaactgac
     8341 ccgcatggat acgctggccg gggaactgtt aaccctgtcc cgcctcgaaa cctccaatat
     8401 ggctttggaa aaagaaagcc tgaaactcct gcccttcctg ggcaacctgg tagaagacaa
     8461 ccaaagtatc gcccaaaaga acggacagac ggtcgccctg tctgccgacg gaaaaatccc
     8521 cgaaaacaca accatccttg ccaacgaaag ctacctgtac cgcgccttcg acaacgtcat
     8581 ccgcaacgcc gtcaactaca gtcccgaagg cagcaccatc ctgatcaaca tcggacaaga
     8641 ccacaaacac tggataatcg acgttaccga caacggcccc ggcgtggacg aaatgcagct
     8701 cccgcacatc ttcaccgctt tctaccgtgc agactccagc gccaacaaac ccggaacagg
     8761 actggggctt gcattgaccc aacatattat tgaacagcac tgcggcaaaa tcatcgccga
     8821 aaacatcaaa ccgaacggtc tgcggatgcg ctttatcctg cccaagaaaa aaaccggttt
     8881 caaaacagaa aaaagtgcga actgaccata atgccgtctg aacccgcttc agacggcatt
     8941 gcacaaacag ttaccccaaa acaaacacac agcgggtgca tctccaaaaa tcacgcacat
     9001 accgtccgca aaggaatata tcatgccggc acaaaccgat ccgggctact tcttcatgcc
     9061 caaccacatt atcctgatag gcgcgagcga acaaccgtac agcctgggtg aacgcgtact
     9121 cagcaacctg ctgagtacgc cctttcaagg aaaaatcacc cccgtaaacc cgcgccacca
     9181 caccatagcc gggcttcccg cctacaccag cctcaacaaa atccccggca gtgcagacct
     9241 gattattacc gttaccccgc ccgacagtta cgacaccctc ctcaaaacct gccgtaaaaa
     9301 gcagctccga cacatcatcc tcatacagga ctgggacagc ctgtctgccg cagaactgca
     9361 caccgccgaa actgccatcc gcaaacacca cggcaacgga ctcaacatca ccgcctacac
     9421 caccgcaggc atccaactgc cctcactcgg actcaacatc agtacccaag acggatatgc
     9481 cgcaggccat accgccatac tgaccggcaa tgccgccgtc agccgccaaa tcgacaacat
     9541 cctgaacaaa ctccgtcaag gcacatcccg ccacatcaac ctgcatcccg gcatcagccc
     9601 catcacatcc gccgattggc tcaaccgctt cggacacagc ctgcacacca aaaccgccgt
     9661 cctacaccac aaccctgaag aggatcagcg caggctgttc agcgcaatcc gccaatttac
     9721 ccgccatacg ccgctgattc tccacatcac ctgcctcacg acagaaaccg accgtgccgt
     9781 actgcactgc ctcgcccgac actgcaactt cctcatcagt ttcaacgccg acgacctcga
     9841 agccgcactg agcgcccaac tgtccgacct tccacccctg tcccgactcg acatcctgtc
     9901 cgacacgcct gccgaatggc tgcacgcgca cgcaccaaaa aacctcaccc tccactttcc
     9961 caaccttccc caccacatcc gcaacggaca cctgaccggc acacccacac cctcaatctg
    10021 ccacgacatc gcctcacgtc agcttgcccg ccccgacacc caagccgtcc tgaccatcct
    10081 cggtccctcc ggacacgagg attacaaaaa aacagcacgc gcccttatcc gcctgtccga
    10141 acagaccgcc aaacccctgc tcgtcaacag ccccttttca gacggcataa cacatttcga
    10201 cacccccgca caggcaatcc gcaccctttc ctaccgtaac accgccgccg ccctgaaaca
    10261 ggcacagctc gacattgcac cgccgcagcc atgccgtctg aaaacaccgc aaccccaaaa
    10321 catcaaaaaa gcccttgcag cggcaaatcc cgccctgctc gccgaagccc tgcacctccc
    10381 cctaccggca caccacccat aacgccgtcc aattccaatt cggcagccac cccctctatg
    10441 gcgacatcct gaccgcacgc tgcaacggac aaaccactgc cgtactcccg ccgtttacca
    10501 cgctcgacag ccgccacctt gcccgctttg ccgaactcga cggcacacaa accctcgatc
    10561 agttcctgca cacactgacc gtcatccccg aataccgcca acacattttc ggcatcaccc
    10621 tcaacctcaa cggcggacaa tacagcagcg atttcatctt aaagacaccc ggaacacacg
    10681 acacgcccaa acgcaagaac acaggcaaag ccgcccaaac cctcgaacat gccgccgcaa
    10741 aaatgcagag tgccgccgca tacctggaac acaaaaaccc gacagccgcc gaatttctcc
    10801 gccacacaag cgaagccgcc gcagaactgc tcggcagcaa aaccgaaacc ggagcagccg
    10861 tacccaacgt acttgccccc tatcccgcag cacaccccaa aacactgtcc ctaaaaaaca
    10921 acacgaccgt taccattatc ccccttttac ccgaagacgc agaagccaaa cagcagttcg
    10981 tccgcagcct cggccccgaa gcacggtaca cacgcttcat gacccacacc aacgaactgc
    11041 ccgcagccac gttggcacgc ctgtgcaacc ccgattacca ctgtgaaccg catggacggc
    11101 aagggatgcc gacagcaaca tcgtcgccgt cgtccgccac agccgcctga accgcaacga
    11161 atgcgaattt ggcatcacac tggcggaaca tatgcgcggc agcgggctgg cacagaaaat
    11221 gatggaactc atcatccaaa ccgccgcaca gcaaggctac ctgagcatga gtgccgacat
    11281 tctcaaaacc aataccccca tgatcaaact tgctgaaaaa tcaggattta ccctcaagga
    11341 atcggacacc gaaaaaaacc tgtaccgcgc atatctgaac cttgcggcag acaaaacaac
    11401 aggaaaaaca aataaaaact tgcgcaccgg ccacaaaata acctaaaatc ggcagtttcc
    11461 atatatccga ttttctcaaa aggactcaaa atggtagtta tccgtttggc acgcggcggc
    11521 tcgaaacacc gccccttcta caacgtcatc gttactgact cacgcagccg ccgcgacggc
    11581 cgcttcatcg aacgcgtagg cttctacaac cccgtagcca acgaaaaaca agagcgcgtc
    11641 cgcctcaatg cagaccgcct gaaccactgg attgcacaag gcgcgcaagt cagcgactcc
    11701 gttgcaaaac tgattaaaga acaaaaagcc gtctaatccg cgtttgccgc catgacagac
    11761 actcaaaacc gggtagccat gggctacatc aaaggcgtat tcggcataaa aggctggctg
    11821 aaaattgccg ccaacaccga atattccgac agccttttgg actaccccga gtggcatttg
    11881 gccaaggacg gcaaaaccgt cagcgttacc cttgaagccg gaaaagtcgt caacggcgaa
    11941 ctccaagtca aattcgaagg catagacgac cgcgattcag cattctcatt gcgcggttac
    12001 accatcgaaa taccccgtga agcattcgcc ccgacagaag aagacgaata ctactgggca
    12061 gacttggtcg gcatgaccgt cgtcaacaaa gacgataccg ttttaggcaa ggtaagcaac
    12121 ctgatggaaa ccggcgcaaa cgacgtattg atgattgacg gagaacacgg gcagattctg
    12181 attccgttcg tttcccaata tatcgaaacc gtcgataccg gcagcaagac cattactgcc
    12241 gactggggtt tggactactg atgcttatcc aggcagttac cattttcccc gaaatgttcg
    12301 acagcattac ccgctacggc gtaacgggac gcgcgaacag acagggaatc tggcagtttg
    12361 aagcagtcaa tccccgaaag tttgccgaca acagattggg ctatatcgac gaccgcccgt
    12421 tcggcggcgg tccgggaatg attatgatgg ctccgccgct tcatgcggcg atagaacacg
    12481 ccaaagcaca atcttcccaa accgcaaaag tcatctacct cagcccccaa ggaaaaccg
//
LOCUS       DAXDSD010000070        12213 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXDSD010000070 DAXDSD010000000
VERSION     DAXDSD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12213
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.070"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            269..1783
                     /gene="katA"
                     /locus_tag="ACFB2Q_001514"
     CDS             269..1783
                     /gene="katA"
                     /locus_tag="ACFB2Q_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC0918515.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            2299..2865
                     /locus_tag="ACFB2Q_001515"
                     /pseudo
     CDS             2299..2865
                     /locus_tag="ACFB2Q_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
     gene            3087..4250
                     /locus_tag="ACFB2Q_001516"
     CDS             3087..4250
                     /locus_tag="ACFB2Q_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFC0918516.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
     gene            complement(4831..6867)
                     /locus_tag="ACFB2Q_001517"
     CDS             complement(4831..6867)
                     /locus_tag="ACFB2Q_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC0918517.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFAEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSESDECR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(6955..7827)
                     /locus_tag="ACFB2Q_001518"
     CDS             complement(6955..7827)
                     /locus_tag="ACFB2Q_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC0918518.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIHFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            8093..10483
                     /gene="gyrB"
                     /locus_tag="ACFB2Q_001519"
     CDS             8093..10483
                     /gene="gyrB"
                     /locus_tag="ACFB2Q_001519"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC0918519.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHQFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGAKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPTVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            10582..11967
                     /locus_tag="ACFB2Q_001520"
     CDS             10582..11967
                     /locus_tag="ACFB2Q_001520"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC0918520.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(12038..12136)
                     /locus_tag="ACFB2Q_001521"
     CDS             complement(12038..12136)
                     /locus_tag="ACFB2Q_001521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFC0918521.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         2883 a         3369 c         3334 g         2627 t
ORIGIN      
        1 aatgagcggg ttcggattcc gtccgataac aaaaaacaaa cgaaaatgga aacttgattg
       61 cttataacaa tattaaatca atttcatagt tttaataacg aaaatcttgg cgtataatcg
      121 catccatagt ttttgcaaaa ggaaaataaa atgtcgattc aagaaattta ttacaatcaa
      181 gaaaccggtt acgaatacgc tttccgccaa atcgtactgt aagccgtttg ccccccattt
      241 gaccaacctg agaaaaggaa caagagcgat gactacctcc aaatgccccg taacccatct
      301 gaccatgaac aacggcgcgc ccgttgccga caatcaaaac agcctgaccg ccggcccgcg
      361 cggccccctg ctgacgcaag atttgtggct gaatgaaaaa ctcgccgact tcgtgcgcga
      421 agtcatcccc gaacgccgta tgcacgccaa aggttcgggc gcgttcggta cgtttaccgt
      481 aacacacgac atcaccaaat acacccgcgc caaaattttc agcgaagtcg gcaaaaaaac
      541 cgagatgttc gcccgtttca ccaccgtggc aggcgagcgc ggcgcagccg atgcggaacg
      601 cgacatccgc ggttttgcgt tgaaattcta taccgaagaa ggcaactggg atgtggtcgg
      661 caacaacacg cccgtgttct tcctgcgcga cccgcgcaaa ttccccgacc tgaacaaagc
      721 cgtcaaacgc gatccgcgca ccaatatgcg ttccgccaca aacaactggg acttctggac
      781 gctgctgccc gaagccctgc accaagttac catcgtgatg agcgaccgcg gtattcccgc
      841 cagctaccgc catatgcacg gcttcggttc gcatacctac agcttctgga acgaagcggg
      901 cgaacgtttt tgggtgaaat tccatttccg cagccagcaa gggattaaaa acctgaccaa
      961 cgaagaagct gcaaaaatca tcgccgacga ccgcgaaagc catcagcgcg acctgtacga
     1021 agccatcgag cgcggcgagt tcccgaaatg gacgatgtac atccaagtca tgcctgaagc
     1081 cgatgccgaa aaagtgcctt atcatccgtt tgacttgacc aaagtttggc cgaaaaaaga
     1141 ctatccgctg attgaagtgg gcgaattcga gttgaaccgc aatcccgaaa acttcttcgc
     1201 cgatgtggaa caatccgcct tcgcaccgag caacctcgtt cccggtatcg gcgcgagccc
     1261 ggataaaatg ttgcaagccc gtttgttcaa ttacgccgac gcgcaacgtt accgcttggg
     1321 tgtgaacttc cgccaaatcc ccgtcaatcg tccgcgttgc cctgttcaca gcaaccagcg
     1381 cgacggacaa ggccgcgccg acggcaacta cggcagcctg ccgcactacg aacccaacag
     1441 cttcggccaa tggcagcaac aacccgactt cgccgaaccg cctttgaaaa tcaacggcga
     1501 cgcggcacac tgggactacc gccaagacga tgacgactac ttcagccaac cgcgcgcctt
     1561 gttcaacctg atgaacgacg cgcagaaaca ggctttgttt gacaacaccg ccgcagccat
     1621 gggcgatgcg cccgacttca tcaaataccg ccatatccgc aactgctacc gttgcgaccc
     1681 ggcatacggt gaaggcgtag ccaaagccct cggactgacc gtcgaagacg cacaagccgc
     1741 ccgcgcgacc gatcccgcac tgggtcaggc cggtttgctg taagggggca ttatgtggat
     1801 ggaaattgaa gaaatcctgt cccgcgccgt ccgataccgg aaataaccgg gcataaaaat
     1861 gccgtctgaa acgtcgtttg accgtttcag acggcatttc cacacctcgc cccgccgttt
     1921 cagtgggcgt tttttattat agtggattaa atttaaaccg gtacggcgtt gcctcgcctt
     1981 gtcctgattt ttgttaatcc actataaacg caaaatatcc cgtcattccc gcaccatccc
     2041 caccggttca aaccggcacg aaaacttttc cgcgtcattc ccgcgaaagt gggaatccgg
     2101 aacgtaaaat ctaaagaaac cgttttatcc gataagtttc cgtgccgacg gggctggatt
     2161 cccactttcg tgggaatgac gggatttctg tttttgattt tttgtttttg cgggaataac
     2221 gggatgcggg ttttcgtgcg gcgtttttcg cgctttgctg ttttgttata atccgccctt
     2281 tttaaggatg ggtgcggtat gagtctttac gctttgctct tggttgcgct gggaatgtcg
     2341 atggatgcgt ttgccgtcgc gctggcaaag ggtgcggcgg tcaggatgcc cccgcgcaaa
     2401 attgcagcaa cggctttggt gttcggtacg gttgaagcgt tcatgccgct ggcgggctgg
     2461 gtaggcggtt tttatgccaa gccgtttatc agcgaatgag accattgggt ggcttttgtc
     2521 ctgttgggcg gactgggtct gaaaatgatg cgcgaagggt tgtccggcga ggcggaagat
     2581 gtgcgcgaaa gcaaacagga aagcctatgg atgacggttt tgactgcttt tggaaccagt
     2641 attgattcca tgatcgtcgg ggtgggcttg gcgtttatgg aggtaaacat cgcctttgcc
     2701 gccgccgtaa tcggtatggc ggctacggta atggttacca tcgggctgac ggcgggaaag
     2761 gcttttggcg tattgttcgg caggcgtgcg gaatttgccg gaggtttggt gttgattgcc
     2821 atcggtacat ggacgctctt atcgcatttg ggtttgattc aatgatgtcg gaaaataaaa
     2881 tgccgtctga agctttaccg gtttcagacg gcattttgtt aaacttgatt ctctatcggt
     2941 attattgtgt aaagctattg aaaataaaat attgattttc cgcaaagatg gctaaaggtg
     3001 cgataggtac actgaagaaa taatcggcag aacaggtttg cctgccggtt tgaacaataa
     3061 ttttaacctc ccataaggaa tacacgatgt ctttcaaact ccgttacctc gcatcagtat
     3121 tggcattgtc ttcactgttg gcggcatgcg gcggtcagga aaagtctgcg gcaggcgatg
     3181 cttctcctgc ttccgagacc gaagcggctt cccaagtaca ggcctcggaa gccgttcctt
     3241 ccgcttcatc ggcctcgccc gaagaccaag accttttgaa acgcgcgcaa ggtgtattcc
     3301 aacctttgcc gactgtcgaa gaaatgcaga aaatccgtcc gtttaccgaa gaacaggtca
     3361 aactcggaca ccaactctgg tatgaaccac gcctttccaa aggcaatacc gtaagctgca
     3421 actcttgcca caaccttgct tccgccggtg tggacaatat gccgaccagt caggggcaca
     3481 aagggcagtt cggcggacgc aactcgccta ccgcattgaa tgctgcgctg ctgggcagcc
     3541 agttttggga cggacgtgcc gccgatgttg aagaacaggc tggcgggcct ttggtgaatc
     3601 cggtggaaat ggcgaatgat tcgcaagagg cggctgcagc caaaatcgcc aaagttcccg
     3661 aatatcaaga aatgtttaaa aaagcttttc ctgaagacgg cgcggtttcg tttaaaaaca
     3721 tcactaccgc attgggtgcg tttgagcgta ccctgctgac gccgaccaaa tgggacgaat
     3781 acctcaaagg caacgtcaac gccctgagcg aacaggaacg aaaaggcgtg cgcgcgttca
     3841 tggacaacgg ctgtattgcc tgccacaacg gtgtcaacct tggaggcacg accttccaga
     3901 aattcggtct ggtccaaggg ccgtattgga aattcattga agatccgaaa cgcgacaaag
     3961 gccgtgctga cgtaaccaaa aaaaccgaag acgaattttt cttccgtgtt ccggggttgc
     4021 gtaacgtggc taaaacttat ccgtatttcc acaacggcag cgtgtgggag ctggataagg
     4081 cagttaccat catgggtaag gcgcaattgg gtaaagacat tccgaaagaa gatgtggata
     4141 acatcgtcgt attcctgaat gcactttccg gcaatgtttc cgaatcagcg cgcacgatgc
     4201 cggaactgcc cctgaccgca ccgatggaat ctaagccgga caacaaataa tccggccggt
     4261 acgcaaaaat gccgtctgaa acgggaatct gtttcagacg gcattttata tttcgtcgcg
     4321 tcagatacgc agggtatgcc gtagggtgtg cttcagccag ccgtttccac caattcaacc
     4381 aaccatgtcc gggtcttatg cgtttttcac gaatgcgcgg cgtaggcgga gggattggtg
     4441 ggctgaagcc taattcaatg gctgcgttgt gtaagccatg atgcgtttgg ataattaatc
     4501 aatattcttg cgctaccatt accatcggct ttgggggttt gtacttcggc attatgataa
     4561 tcaatagcct gtttggattg tattcccgaa tccgacagtt ttagattata aaaaggccgt
     4621 ctgaaaaatt ttcagacggc cttttatagt aaatttcaaa taaaaatatc tttgtcgtca
     4681 ttcccacgca ggcgggaatc cagacttgtc ggcacagaaa cttatcgggt aaaacggttt
     4741 cttcaatttt atgttccgga ttcccgcctg cgcggggata acgattcagg tatttctaca
     4801 tcgaatccgc tttttatatc aacctgacta tcaagccgcg ttgtcgaagc cgctgtggcg
     4861 cagcagtgcg tctatgctcg gttcgcgtcc gcggaaggcc ttgaaggatt ccgccgcgct
     4921 gcgggagccg ccgacggcga ggatttcctg ccagaagcgt ttgcctgtgg cggcaacgtc
     4981 gtcgctttct tcaaaggcgg cgtaggcgtc ggcgctgagg acttccgccc atgcgtagct
     5041 gtaatagcct gcggagtagc cgccggcgaa gatgtggccg aagctgttgg cgaagcggtt
     5101 gtattcgggc ggttggatga cggcgacttc tttgcgcacg ctgtctaaaa cctgctgcca
     5161 gtttttcaga cggcattcgt cgctttcact gtaaatcatc atatcgaaga gggcgaactc
     5221 catttgccgg acgaggaaca taccgcgctg gaagtttttg gcggcgagca ttttgtcgaa
     5281 gagttctttc ggcaggggct cgccggtttc ttcgtgggcg gacatttgtg ccaagacgtc
     5341 gtattcccag acgaagtttt ccataaactg gctgggcagc tcgactgcgt cccattcgac
     5401 gccgttgatg ccggacacgc ccagttcgtc cacttgggta agcaggtggt gcagtccgtg
     5461 gccggtttcg tggaagaggg tgaggatttc gtcgtggctt aaacgcgctt ctttgccgcc
     5521 gacgggcggg gcgaagttgc agacgaggta ggcggtgggc agttgcagcg tgccgtcggc
     5581 aaagcggcgg cggcctttgt agtcgttcat ccacgcgccg ccgcgtttgc cttcgcgtgc
     5641 gtacaaatcc atataaacgc cgccgatggt ttcgccgttt tgttgcaatt caaaatagcg
     5701 cacgtctttg tgccagacgg gaacggtttt ttcggcgaat ccgatgccgt agagtttttt
     5761 gatttgggcg aacagtccgt ttaatacttt gccgacgggg aagtattttt tgacttcggt
     5821 ttcgctgaat gcgtatttgg cttcgcgcag tttttcgccg gcgtagctca agtcccacgg
     5881 ctgcgggtcg gcgagaccga ggtgttcgcg ggcgaaggct ttgacttcgg cgaggtcttt
     5941 ttcggcgtag ggtttggcgc ggcgggcgag gtcgtgcagg aagtttaaaa cctgttcggg
     6001 cgtgtccgcc attttggttg ccagcgacaa ttcggcgtaa tttttaaagc cgagcagttt
     6061 ggcggttttc aatgcgtttt cgagcgtgcg gtcgatgttg gcggtgttgt cgaatttgcc
     6121 gtcgtttgaa agttcgctgg cacgggtaac gtaggcgcgg tagatttgtt cgcgcagttc
     6181 gcggttgccg gcgtattgga tgacggcaag gtagtgcgga atctgcaagc cgattttgta
     6241 acctgttttg ccttcgcttt gcgcggcggc ggcaaacatc gcgagcgcgt cttcgggaat
     6301 gccggcaagc ggtgcggcat cgtcaaagta aatgccgaac gcgtcggtcg cgtctaggac
     6361 gttttgggag aatttggcgg aaagttgcgc gccttcggtt tgcagttttg ccagttctgc
     6421 ctgccgttcg ggcggcagtt ccgcgccgct caatacgaaa tcgcgcaggt cgtgatcgag
     6481 cttggttttt tgtgcggggg aaagcgttgc aaattcgggg gaatttttga tggttttgaa
     6541 gcggttgtac agttcgatgt cttgtccgat ttcggtgaag aagacggtga tttcaggcat
     6601 cagttcgtta tagacggcgc gcagttcggg cgtgtcgacg acggagttga gatgggacac
     6661 gacgccccaa atcctgccga cgcgttcggt gatgccggtc agacgctcga cggtgttcgc
     6721 ccagccggtg tgcgtttgcg ctttgacggc ggcgatttgt ccgcgcgctt cggcgatggc
     6781 ggtttggacg gcgggtttga tgtcttcggt tttgatttga ttaaaacggg gttcttcgcc
     6841 caagtggagc agtgcgttgt caatcataag atgggtttcc tttgcgtgtt gtgcttcaga
     6901 cggcatatcg gcaatatgcc gtctgaagcc agaaatgggg ccggccgggg aaagttaaag
     6961 tgtttcggag ccggcgggat gatttaacag gcggtattgt acgcgcaaaa attcgtccat
     7021 aaccgcctgt tggatttcca gccgctttga aacgggggag gcgaagcgga cgatgatgcg
     7081 gtatgccttg tcgtcgtacg gtacgcgggt aacgcgcggc ctggcggcgg gcgtgataaa
     7141 cagtttttcc gcctgcacgt tttccaaata ccgctgaatg gcggggatgt agggcgcgca
     7201 caagggctcg agtacggctt tcagacggca tacggcttca tccgaatcca aatggatggg
     7261 aacggggatt tcgaccgtat ggatgacata gtcgcccaaa atattgtcgc ggcgcacggg
     7321 gtggctcaac aacaggctgt tggggaaaga aacggtggtt cccgcaagct gtccgaccaa
     7381 ggggttcgga ccgacctgca tcatcagcgt gttcaacaga ttgatgtcga ccacgcgccc
     7441 gcgcaggccg ttgatttcga tatagtcgcc gaccgagtat tgctgggtgg cagaccttaa
     7501 aatactgccc gacagacaca taatcagttc ttttgtcgcc acgacgaccg ccgccgccac
     7561 cgcaaacatc gacaaagcca gcgtttgaat ttgcgccgac cagataaatg ccagcgaaaa
     7621 cagcaccaaa agcagcgtta tattgcggct ggcaaccaaa aaccgccgct tgctttcgat
     7681 gccgaaatcc ggatgccgtc tgaagtggat attcaacaga agggcgcgcg ccagcagcaa
     7741 agccgcgacc gccgccacgg attcggccgc ctccgcgcgt atcgggacgg catcgggcca
     7801 agtgttcaac atattccata tttccatttc cgcgcccctg tccgaaaagt atagggtgta
     7861 gattttagtg gcaaaaaaac gcttttgcca ctttgccggc aaattccgcg cccgtaccgg
     7921 ccgcaaatgc gccgcacgcc ctttcagacg gcatccggat gcggataacg ctgaatcagg
     7981 cggcgaaatc ggctataata cacaattata tgcgccgtcc cgcccgcttt ttccacaagg
     8041 tgcggactgt tttggcggca tcgcaaaatc ttttcaaaat ccggcaaaaa atatgactga
     8101 acaaaaacac gaagaatacg gcgccgacag catccaggtg ctcgaaggct tggaagcggt
     8161 acgcaaacgc cccggcatgt acatcggcga cacgcaggac ggcagcgggc tgcaccatat
     8221 ggtgtttgaa gtattggaca acgccatcga cgaagcactc gccggacatt gcgacaaaat
     8281 cacggtaacg atacacgccg accattccgt cagcgtcgcc gacaacgggc gcggtatgcc
     8341 caccggcatc cacccgaaag aagggcgttc cgccgccgaa gtcatcatga ccgtcttgca
     8401 cgcgggcggc aaattcgaca acaacagcta caaaatctcc ggcggcctgc acggcgtggg
     8461 cgtatccgtc gtcaacgcgc tgtccgactg ggtaacgctg accatctacc gcgacggcaa
     8521 agaacacttc gtccgcttcg tacgcggcga aaccgaagag ccgctgaaaa ttgtcggcga
     8581 ttccgacaaa aaaggcacga ccgtgcgctt cctcgccggc acggaaacct tcggcaatat
     8641 cgaatacagc ttcgacatcc tcgccaaacg tattcgcgaa ctttcgttcc taaacaacgg
     8701 cgtggacatc gaattgaccg acgagcgcga cggcaagcac gaaagcttcg ccctttccgg
     8761 cggcgtggcg ggcttcgtgc aatacatgaa ccgcaaaaaa acgcccttgc acgaaaaaat
     8821 cttctatgcg ttcggcgaga aagacggcat gagcgtcgaa tgcgcaatgc aatggaacga
     8881 cagctatcag gaaagcgtgc agtgcttcac caacaacatc cctcagcgcg acggcggtac
     8941 gcacctgacc gcgctgcgcc aagtgatgac gcgcaccatc aacagctaca tcgaagctaa
     9001 cgaagtcgcc aaaaaagcca aagtggaaac cgccggcgac gatatgcgcg aaggtttgac
     9061 ctgcgtgttg tccgtcaaac tgcccgaccc caaattctca tcccaaacca aagacaaact
     9121 ggtttccggc gaaatcggcc ccgttgtcaa cgaagtcatc aaccaagcac taaccgactt
     9181 cctcgaagaa aatccgaacg aagccaaaat catcaccggc aaaatcgtcg atgccgcccg
     9241 cgcacgcgaa gccgcccgca aagcccgcga aatcacccgc cgcaaaggcg tgatggacgg
     9301 cttgggactg cccggcaaac tcgccgactg ccaagaaaaa gaccctgccc tgtctgaact
     9361 ctacctcgtc gagggcgact ccgcaggcgg ttccgccatg cagggccgcg accgcaaatt
     9421 ccaagcgatt ttgccgctca aaggtaaaat tttgaacgtc gaaaaagcac gttttgaaaa
     9481 aatgctcgcc agccaagagg tcgccaccct gattaccgcg ctgggtgcag gcatcggcaa
     9541 agaagagttc aaccctgaaa aactacgcta ccaccgcatc atcatcatga ccgatgccga
     9601 cgtggacggt gcgcacatcc gcaccctgct cctgaccttc ttctaccgcc aaatgcccga
     9661 actggtcgag cgcggctaca tttacatcgc ccagccgccg ctctacaaag ccaaatacgg
     9721 caagcaggag cgttacctca aagacgaact ggaaaaagac caatggctgc tcggccttgc
     9781 cttggaaaaa gccaaaatcg tttcagacgg ccgcaccatc gaaggcgcag aacttgccga
     9841 caccgccaaa caattcttgt tggcgaaaac cgtcatcgaa caggaaagcc gcttcgtgga
     9901 cgaactcgtc ctgcgtgcca tgctgcacgc gtcgcccatt gatttgacgt cgtctgaaaa
     9961 cgccgataaa gccgttgccg aactttccgg tttgcttgac gaaaaagaag ccgccctcga
    10021 acgcatcgaa ggtcatgaag gacaccagtt catcaaaatc acgcgcaagc tgcacggcaa
    10081 cgtcatggtc agctacatcg aacccaagtt cctcaacagc aaagcctacc aaaccctcac
    10141 ccaaaccgcc gccgcgctca aaggcttggt cggcgagggc gccaagctct acaaaggcga
    10201 gaacgagtac gacgcggaca gctttgaaac cgctttggac atcttgatga gcgttgccca
    10261 aaaaggtatg tccatccaac gatacaaagg tttgggcgag atgaaccccg agcagctttg
    10321 ggaaaccacg atggatccca ccgtgcgccg cctgttgaaa gtgcgcatcg aagatgccat
    10381 tgccgccgac gaagtgttcg ttaccctgat gggcgacgag gtcgaaccgc gccgcgcctt
    10441 tatcgaaaac aatgcgctga ttgcgcaaaa tatcgacgca taagtgccgt tttaaaaaag
    10501 gagacgggca gcttgccgcg tctccttttg gtttgtcaaa cggaagccat gccgtctgaa
    10561 accgcctttc ggagcaaaac catgatcagc atttttgata ttttcaaaat cggtatcggc
    10621 ccttccagtt cgcatacggt cggcccgatg aaggccgccg ccgcctttgc cgcaggcttg
    10681 gacgcgcaaa ccgcccgcat cgccatcgac atttacggct cgcttgccct gaccggatac
    10741 ggacacggca catttgacgc gctgatgctc ggtttggaag gcagcctgcc gcacgacatc
    10801 ccgcttgccg gcattcccga acgcctcgga cgcatccgca cgcagcacat cctccggctc
    10861 aacgggcaag aaatccgctt catccccgac cgcgacctga acatacgcgg caatcaagtg
    10921 ctgcccaaac accccaacgg cctgcgattt accgcctatg cttcagacgg cacggtattg
    10981 aaggaacagg tttattattc ggtcggcggc ggctttgtcg ttaccgaaga agacttcgag
    11041 tggcagtcgg aaacggaaaa agccgttcct tatccttaca acagttgtgc cgggctgctt
    11101 gcccgatgcc gtctgaaccg gctcgacatc tccgaagtcg tgttggcaaa cgaagccgcg
    11161 cttgccggat gcggcgaagc cgaaatccgc cgccgcgtcg ctgccgttgc cgaggttatg
    11221 gaaggctgca tcaaacgcgg cttgggtgcg gacggcgaac tgcccggcgg attgaacgtc
    11281 cgccgccgcg ccccgcagct tgccgccaaa ctcaaagtcc tgcgcgaaac cgaaatcgtc
    11341 aacacccagc tctggccgat ggtgtacgcc atggcggtca acgaagaaaa cgccgccggc
    11401 ggacgcgtcg ttaccgcccc gaccaacggc gcggcaggca tcattcccgc cgtattgcat
    11461 tatttccgca agttcaaccc ccacgccaca caggaacgtg tcgaaaactt cctgctcacc
    11521 gcaggcgcaa tcggcatcct ctacaaaacc aacgcctcca tttccggcgc ggatgtcggt
    11581 tgtcagggcg aagtcggcgt agcgtgttca atggcggcgg gtgcatacgc cgaagtcatc
    11641 ggcggcacgc ccaaacaagt ggaaaacgcc gccgaaatgg caatggaaca ccatttgggc
    11701 ttaacttgcg accccgtcgg cgggttggtg caaatcccct gcatcgaacg caacggcatc
    11761 gccgccgaaa aagccctcaa actcggcacg ctcgcgctct tggaagacgg cacggacaaa
    11821 aaagtctcgc tcgacgaagt catccgaacc atgctgcaaa ccggccggga catgaaggcg
    11881 acctacaaag aaacctcgct tgccggactc gccgccacgc tccggaaaaa agccgtcccc
    11941 gtatccgtgc gcgtggtcga gtgctgagga tggacagaaa cgaaaatgcc gtctgaaaac
    12001 agtttttcag acggcatttc atttattttt tgcgggttta ccgcttcaac gccaacgtca
    12061 acacgcccgc cgtaaccagc cccaagccta tccattcctg cgtgttcggg cgttcgtcca
    12121 agaaaaccac cgccatcagg aatgcaaacc ggcgttgttt gcagcatccg gcaagaattt
    12181 ttatagtgga ttaacaaaaa tcaggacaag gcg
//
LOCUS       DAXDSD010000071        11942 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXDSD010000071 DAXDSD010000000
VERSION     DAXDSD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11942
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.071"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            363..1109
                     /gene="pssA"
                     /locus_tag="ACFB2Q_001522"
     CDS             363..1109
                     /gene="pssA"
                     /locus_tag="ACFB2Q_001522"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC0918522.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            1110..1916
                     /locus_tag="ACFB2Q_001523"
     CDS             1110..1916
                     /locus_tag="ACFB2Q_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC0918523.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQTVDWKTIFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(2539..2991)
                     /gene="rplI"
                     /locus_tag="ACFB2Q_001524"
     CDS             complement(2539..2991)
                     /gene="rplI"
                     /locus_tag="ACFB2Q_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC0918524.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(3008..3238)
                     /gene="rpsR"
                     /locus_tag="ACFB2Q_001525"
     CDS             complement(3008..3238)
                     /gene="rpsR"
                     /locus_tag="ACFB2Q_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC0918525.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            complement(3245..3547)
                     /gene="priB"
                     /locus_tag="ACFB2Q_001526"
     CDS             complement(3245..3547)
                     /gene="priB"
                     /locus_tag="ACFB2Q_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC0918526.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            complement(3548..3916)
                     /gene="rpsF"
                     /locus_tag="ACFB2Q_001527"
     CDS             complement(3548..3916)
                     /gene="rpsF"
                     /locus_tag="ACFB2Q_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC0918527.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVGELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLVGAAEEAAAQ"
     gene            complement(4072..5022)
                     /gene="trxB"
                     /locus_tag="ACFB2Q_001528"
     CDS             complement(4072..5022)
                     /gene="trxB"
                     /locus_tag="ACFB2Q_001528"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC0918528.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5161..7338
                     /locus_tag="ACFB2Q_001529"
     CDS             5161..7338
                     /locus_tag="ACFB2Q_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC0918529.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            7393..9279
                     /gene="uvrC"
                     /locus_tag="ACFB2Q_001530"
     CDS             7393..9279
                     /gene="uvrC"
                     /locus_tag="ACFB2Q_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC0918530.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSDQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            9396..10800
                     /locus_tag="ACFB2Q_001531"
                     /pseudo
     CDS             9396..10800
                     /locus_tag="ACFB2Q_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            10922..11638
                     /gene="trmB"
                     /locus_tag="ACFB2Q_001532"
     CDS             10922..11638
                     /gene="trmB"
                     /locus_tag="ACFB2Q_001532"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC0918531.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVKNMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
BASE COUNT         3003 a         3438 c         2887 g         2614 t
ORIGIN      
        1 aatttatccc gcttcaaaat cagcatacgg tcggaaatgc aaaaaatatc tttcaatttg
       61 ttgaaacctg caccccccaa aaaataggga aataccgccc cggtttgaac ggcgcgccgc
      121 atattccgat gccttccctc cgataccttc cggcaagcgc agaaatgccc ggcaacaata
      181 tccatccggc aaaatccgaa acaacacacc cggtggcagg cagagtcaaa ccgccccgca
      241 aagcatccgc catcagaaaa acaaaccgcc tccgagggct tcatcctaaa gggcgtattg
      301 ttcgataatg gtttgggtta taatccccta tcgattctcc acgtccgtga gacacttcag
      361 ctatggaaac cccgaccaac accccgcaac gctccctgcg tcaaaacagt atctacctgc
      421 tgcccaattc ctttaccatc gccgcgctgt tttccgcgtt ttacgccatt acccaatcca
      481 tgcacggacg ttatgaaacc gccgccatcg cggtattcat ctctatgctg ctggacggta
      541 tggacgggcg cgtggcgcgg ctgaccaaca gccaaagcgc gttcggggag cagctcgaca
      601 gccttgccga catggtcagc ttcggcgttg cccccgcgct tattgcctac aaatggcagc
      661 tttggcagtt cggcaaaatc ggttattccg tcgccttcat ctactgcgcc tgcgccgccc
      721 tacgcctcgc gctgttcaac acgctcatcg gcaaggtgga caaacgctgg tttatcggcg
      781 tacccagtcc gactgccgcc gcgctgattg tcgggctgat ttgggtcaac cacagcgtcg
      841 aaaaattccc cgccgtccac tggtgggcat taggcatcac actgtttgcc ggcctgtcga
      901 tgattgtcca aatccctttt tggagtttta aagaaatcaa catccgcaga caagtcccct
      961 ttgtcggaat gctgcttgcc gtcttactgc tgcttctggt cacttgggaa ccgtcgctcg
     1021 tccttttcct gttctttctc ggatacagcc tgtccggcta cattatggcg gcacgccgat
     1081 tttggaaaaa atacagaaag gcggattaaa tgtggcattg ggacattatc ttaatcctgc
     1141 ttgccgtagg cagtgcggca ggttttattg ccggcctgtt cggtgtaggc ggcggtacgc
     1201 tgattgtccc tgtcgtttta tgggtgcttg atttgcaggg tttggcacaa catccttacg
     1261 cgcaacacct cgccgtcggc acatccttcg ccgtcatggt cttcaccgcc ttttccagta
     1321 tgttggggca gcacaaaaaa cagacggtcg actggaaaac catatttgcg atgatgccgg
     1381 gtatgatatt cggcgtattc gctggcgcac tctccgcaaa atatatcccc gcgttcgggc
     1441 ttcaaatttt cttcatcctg tttttaaccg ccgtcgcatt caaaacactg cataccggtc
     1501 gtcagacggc atcccgcccg ctgcccgggc tgcccggact gactgcggtt tccacactgt
     1561 tcggcgcaat gtcgagctgg gtcggcatag gcggcggttc actttccgtc cccttcttaa
     1621 tccactgcgg cttccccgcc cataaagcca tcggcacatc atccggcctt gcctggccga
     1681 ttgcactctc cggcgcaata tcgtatctgg tcaacggtct gaatattgca ggattgcccg
     1741 aagggtcgct gggcttcctt tacctgcccg ccgtcgccgt cctcagcgcg gcaactattg
     1801 cctttgcccc gctcggtgtc aaaaccgccc acaaactttc ttctgccaaa ctcaaagaat
     1861 ccttcggcat tatgttgctt ttgattgccg gaaaaatgct gtacaacctg ctttaaaaac
     1921 cttcttaccg tttgcacaag caattagtca ggacaaagct gccctacctc ccgttccgac
     1981 aaggggatag gcagtctgag cgtgcgcccg acttattgcc tttcaccacc caatcgacac
     2041 ctacctgcgg attccattgc ctttttccaa actgccgatt tccctgcctt cacgcccgaa
     2101 ggcatcggcg catcgcctgc cgtgccgact ccatgcgatg cgactgcctc ccaaacggta
     2161 tttccgccat aatgaccggc atacaagccg caacctttgc aatcatataa gttattaaat
     2221 tttttaggat gtattttgac aatctatttc aactatttac aagagaaaag acaattattt
     2281 tttcggttgg gaagagacct atcctattga atatattgaa accaggtacg cttgtcaaca
     2341 ctatattaaa acacagccaa tattttttgg cgcattttat gcatcaaatt tcgttaacaa
     2401 accatttctg caaagacctc aggcaaataa aaaacaaccg tcccgatttt tgcagaatat
     2461 acggaaaaca aaacaaatgc cgtctgaaac cacattctga taatcggcag ggtttcagac
     2521 ggcatctgat aatttcaatt actcggctgc ggcaatgacg gcaacagtaa ttttagcaac
     2581 ggcatcagtg tgtaaagcca cttccacttc gtactctcca acggctttca gaggaccgtt
     2641 cggcagacgt acatttgctt tcacggcttc gatgccggca gcaacgattg cagcagcaat
     2701 gtcggcattg gtaacggaac cgaacaggcg gccgtccaca ccggctttct gtgcaacggt
     2761 aacggtttga ccgtccagtt tttcctgacg ggctcgggca tctgccaaaa tttcggcctg
     2821 tttggcttcc aattcggcgc ggcgtgcttc aaactctttc atgtttgctt cagtagcacg
     2881 ttttgcctta ccagcaggaa tcaagaagtt gcgggcataa ccgtttttta cggtaacgat
     2941 gtcgcccaaa ttacccaaac cgccgatttt ttctaacaga ataatttgca tgatttaaac
     3001 tccaaaatta tttgtgttgg tcggtataag gcaggagtgc caagaagcgc gcgcgtttta
     3061 cggcaacagc caattggcgt tggtagaatg ccttcgttcc tgtgatgcgg gcaggaatga
     3121 ttttaccgtt ttcagagata aagtctttca gcaaatcaac ttgtttgtaa tcgacttctt
     3181 ggattttttc agccgtgaaa cggcagaatt ttctacgttt gaatgattga cgagccattg
     3241 tcgtttaacc tttatattct ttaatatttt gtatcctgag catcggcatc agggaacgtc
     3301 tgcttttttg agctaaaaaa ccttcgacgt gaacatatac accttgccga tactgccact
     3361 cttccgcctg cctacccaaa atccgcgcgg ggatttccaa ttggacaagg cattgctgcc
     3421 cattttcctc ctgccacgat tcgtgcttta aaataatatc taaaacaggg attccggcag
     3481 gcgtatatcg aatagggaaa gccttttcaa tcagcgcggc aagcgaaaca agattagtga
     3541 atcccaatta ttgggcagcc gcttcttcag ccgcgccgac caacaggttc ttagcctttt
     3601 cgccgcccag cataggggag gcttcggtaa cggcgtgttt ggttttgatg gtcagatgac
     3661 gcaataccgc atcattgaaa cggaacgcgg tttccaactc cccaacaact tcgggagtgg
     3721 tttcgatgtt catcaaaaca taatgtgctt tatggatttt gttaatcggg taagccagtt
     3781 ggcggcggcc ccaatcttcc aaacggtgaa tcttaccgtt tgcctcggta atcatggttt
     3841 tgtaacgttc aaccatagcg ggcacttgct cgctttgatc aggatgaacg ataaacacga
     3901 tctcgtaatg acgcatgtta tctccttatg gatggtaaaa acagccttct gccatgcgaa
     3961 agcagaaggc aaggttcaaa tagcaggcat tatattgggg tttgccgacg gaatcaagga
     4021 tttggtacga aaaacttgca ttccgccgaa aatttcggtt tcagacggca ttcaaatgtt
     4081 ttggctgccc agccaacgtt ccgcgtccaa agccgcctga cagccggagg ccgcgctggt
     4141 aattgcctga cggtaggtat ggtcttttac gtcgcccgcc gcccatacgc cttcgatatt
     4201 ggttgcaccg acattgtccg ccgtgccgcc ttttgttttc aggtagccgg cttcgtccat
     4261 ttccaactgc cctttgaaaa tatcggtatt cggcttgtgc ccgatggcga taaagatgcc
     4321 gctgacggca atttgttgat cagaaccgtc gttgtttttt aataatgcgc catttacgcc
     4381 ccgatcgtcg cccagtactt cttgcaggtt gctttccagc ttgaggatga ttttgccctc
     4441 ttccacgcgt ttcatcagtt tgtcaatcat gattttttca gcacggaact cgctgcggcg
     4501 gtgaatcagc gtaacggttt tggcgatatt ggcaaggtag agtgcctcct caactgccgt
     4561 attgccgccg ccgactacgg caacatcttg gtttttgtag aagaaaccgt cgcaggtggc
     4621 gcaggcggaa acgcctttcc ctgcaaacgc ttcctcactc ggcaaaccaa ggtatttggc
     4681 ggacgcgccg gtggcgacaa tcagggcatc gcaagtgtac tcgcccatat cgcctttgag
     4741 tgcgaacggg cgtttttgca gatcgacggc gttgatttgg tcaaaaatga tttccgttcc
     4801 gaaacgttcg gcgtgggcga gaaaccgcgc catcaattcc ggcccttgca cgccgtcggc
     4861 atcggcaggc cagttgtcca cttcagtcgt tgtcatcagt tgcccgcctt gcgcgatacc
     4921 tgtaataatg acggggttta aattggcacg tgcggcatag acggcggcgg tgtatccggc
     4981 ggggccggaa cccaaaataa tcagtttgcg gtgttgggac attgtttttc ctttgctgtg
     5041 tcaagttttc ggattctacc cgaattatcg gcgcgtttga gaaattttga ccataccggc
     5101 gttcagacgg catcccgcag ccttgactgc cgtctgaaca tcaaaacggg aatcaaactt
     5161 atgcaacaaa aaatccgttt ccaaatcgag gggatgacct gtcaggcatg tgcttcgcgc
     5221 attgaaaaag tgttgaacaa aaaagatttt gtcgaatcgg cgggagtgaa ctttgccagt
     5281 gaggaagcgc aggttacgtt tgacggcagc aaaacctcgg ttgccgacat tgccaaaatc
     5341 attgagaaaa ccggttacgg cgcgaaggaa aaaacggaag atacattgcc gcaacctgaa
     5401 gcagaacacc atatcggctg gcggttgtgg cttttgctga ccatcaatat cccgttcctt
     5461 atcggtatgg tagggatgat gctaaaaggg ctgaattgga cacggcacga ttggatgatt
     5521 ccgcctgtat ggcagtttgt actggcaagc atagtgcaac tttggctggc aatcccgttt
     5581 tacaaaagcg cgtgggcaag cattaaaggc gggctggcga atatggacgt actcgttacc
     5641 atcggcacgg tgtcgattta cctgtattcc gtttatatgc tgtttttcag ttcgcatgcg
     5701 gcgcacggta tggcgcatgt gtattttgaa gcgggcgtga tggtgatcgg ttttgtgtcg
     5761 ctgggtaagt ttttggaaca ccgcaccaaa aaatccagcc tgaacagctt gggcttactg
     5821 ctaaaactca cgccgaccca agtcaacgtg caacgcaacg gcgaatggaa acaactgccc
     5881 atcgaccaag tgcaaatcgg cgaccttatc cgcaccaacc acggcgaacg catcgctgcc
     5941 gacggcatta tcgaaagcgg cagcggttgg gcggacgaaa gccaccttac cggcgaatcc
     6001 aatcccgaag agaaaaaggc gggcggcaaa gtgttggcgg gcgcgctgat gaccgaaggc
     6061 agcgtggtgt accgcgccgc gcagctcggc agccaaaccc tgctcggcga catgatgaac
     6121 gcgctctctg aagcacaagg cagtaaagca ccgattgcgc gcgtggccga taaagcggcg
     6181 gcggtatttg tgccaactgt cgtgggcatc gcgcttctga cttttatcgt tacttggctg
     6241 attaagggcg attggacggt cgcactgatg cacgccgttg ccgttttggt gattgcctgc
     6301 ccgtgcgcgc tcggtctggc gacccctgcc gcgattatgg tcggcatggg caaagcggtg
     6361 aaacacggca tttggtttaa agacgcggcg gcaatggagg aagcagccca cgtcgatgcc
     6421 gtcgtattgg acaaaaccgg tacgctgacc gaaggcaggc cgcaggttgc cgccgtttat
     6481 tacgttcccg acagcggctt tgacgaagac gctttgtacc gcatcgccgc cgccgtcgag
     6541 caaaacgccg cccacccgct cgcccgcgcc atcgtctccg ccgcacaagc gcgcggtttg
     6601 gagattcccg ctgcacaaaa tgcgcaaacc gttgtcggag caggcattac cgccgaagtg
     6661 gaaggcgtgg gtttggtgaa atcaggcaaa gccgaatttg ccgaactgac cttgccgaag
     6721 ttttcagacg gcgtttggga aatcgccagt gcggttaccg tatctgtaaa cggcaaaccg
     6781 atcggcgcat tcgcactctc cgacgcgttg aaagccgata ccgccgaagc cataggccgt
     6841 ctgaaaaaac acaatattga tgtctatatt atgagcggcg ataaccaaag tacggtcgaa
     6901 tacgtcgcca aacaactggg catcgcacac gccttcggta atatgagtcc gtgcgacaaa
     6961 gccgccgaag tgcagaaact caaagccgcc ggcaaaaccg tggcgatggt cggcgacggc
     7021 atcaacgacg cgcccgcgct tgccgccgcc aacgtcagct tcgccatgaa aggcggtgcg
     7081 gacgttgccg aacacaccgc ctccgccacg ctgatgcagc attcggtcaa tcagctcgcc
     7141 gatgccctgc tgatatcgca ggcaacgttg gaaaacatca agcaaaacct atttttcgcc
     7201 ttcttctaca atatattggg cattccgctc gccgcgctcg gctttttaaa tcccgtcata
     7261 gcaggcgcgg caatggcggc aagctcggtt tcggtattgg gcaatgccct gcgcctgaaa
     7321 tgggtaaaaa tcgattgacc gcatataacc gcccgacagc ttttccgaac ggataaggct
     7381 gccgctgccg atatgccgtc tgaagccgtt tttcaaatga ttgatatgaa tacagaaaac
     7441 cgttctccgg aacaattcga catcccgctc ttcctcaaaa acctgcccaa gctgccgggc
     7501 gtgtaccgtt tttttgacga aggcggcaac gtcttatacg tcggcaaagc ggtcaacctc
     7561 aagcggcgcg tgtccggcta tttccaaaaa aacgaccatt cgccgcgcat cgcgttgatg
     7621 gtgaaacagg ttcgccacat cgaaaccacc atcacgcgtt ccgaagccga agccctgatt
     7681 ctcgaaaaca acttcatcaa agccttgtcg ccgaaataca atatcctttt tcgcgacgac
     7741 aaaagctatc cttatttgat gctcagcggc catcaatatc cgcaaatggc gtattaccgc
     7801 ggcacgctga aaaatcccaa ccaatacttc ggcccgtatc cgaacagcaa cgccgtgcgc
     7861 gacagcattc aagtgttgca aaaagtcttc atgctgcgta cctgcgaaga cagcgtgttc
     7921 gaacaccgcg accgcccttg cctactgtac caaatcaaac gctgcaccgc gccctgcgtc
     7981 ggacacatca gcgaagaaga ctactgcgac agcgtgcgtc aagccgccac tttcctcaac
     8041 ggcaaaaccg acgaactgac ccgcaccctg caacacaaaa tgcagaccgc cgccgcgaat
     8101 ctgcaatttg aagaagccgc ccgttatcgc gaccaaatcc aagcactcgg cattatacag
     8161 agcgaccagt tcatcgacag caaaaacccg aacaacccca acgatatcga cctgctcgcg
     8221 ctcgccgttt cagacggcct tgtctgcgta cactgggtca gcatccgcgg cggacggcac
     8281 gtcggcgaca aaagcttctt tcccgacacc aaaaacgacc ccgaaccaaa cggtcaagat
     8341 tacgccgaag ccttcgtcgc ccaacactat ctgggcaaaa gcaaacccga catcatcatc
     8401 agcaactttc ccgttcccga tgcgttgaaa gaggctttgg aaggagaaca cggcaagcag
     8461 atgcagttcg ttaccaaaac cataggcgaa cgcaaagttt ggttgaaaat ggcggaacaa
     8521 aacgcacaaa tggcgattac ccaacgccac ctgcaacaaa gcaaccaaca acaccgcatc
     8581 gacgaacttg ccaaaatcct cggcatgaat tcagacggaa tcaaccgcct tgaatgtttc
     8641 gatatcagcc acacgcaagg cgaagccacc atcgcctcct gcgtcgtgta cgacgaacaa
     8701 aacatccaac cctcgcaata ccgccgctac aacatcacca ccgccaaacc cggcgacgac
     8761 tacgccgcca tgcgcgaagt gttgacgcgc cgttatggca aaatacagga agccgaagcc
     8821 aacggcgaaa gcgtcaaatg gccggatgtc gtgttgattg acggcggtaa agggcaaatc
     8881 ggcgtagccg tatccgtatg ggaagaactc gggctgcaca tccctttagt cggtattgcc
     8941 aaaggccccg aacgcaaagc cggcatggaa gaactcatac tgccttttac cggcgaactc
     9001 ttccgcctgc ctcctaatag cccggccttg catttattgc aaaccgtacg cgatgaatca
     9061 caccgctttg ccatcacagg ccaccgtaaa aaacgcgaca aagcacgcgt tacctcgtct
     9121 ctcggcgaca ttcccggtgt aggcagcaaa cgccgccaag ccctgcttac ccgcttcggc
     9181 ggactgcgcg gcgtgattgc cgccagccgc gaggacttgg aaaaagtgga aggcatcagc
     9241 aaggcattgg cggaaaccat ttataatcat ctgcattagt atgccgccaa agcaaaaacc
     9301 gcctgtaaaa atatgataca gcaggccggc ataccgatat aaacctaact tcatgacgaa
     9361 taacgatgat tcgacaaaac ggaaaacgat ctgatatgaa caatcccgac ttaccctatc
     9421 ggcaggcctt ggaacgcctg tctcaaaaac aatattataa ctttaccgaa gtccgccgac
     9481 tgctgacaga agcggcctcg gcagatcatc ccgccgccac attcgagttg gcaaaacacc
     9541 tgatgaacgc ggacagcccg caccaagacc gcgaacaagg tatggaaatg ctccgcatcg
     9601 ccgccgaaca ggggcatccc tatgcgcgtt acaatttggc atatatccaa gaattggaag
     9661 gcgcgccccc ggaaaccctg ataccgcttt acaggcctct ggcagaagca ggcctgcccg
     9721 aagcgcaagt ccgcctgatg taccttctgt acgcgtcccg acattttgaa gaagccctgg
     9781 aatgggcaaa acaagcgcaa aaaacaacaa tccccacggg caatacctgc tcgcccaata
     9841 ctgccggcac ggcacgccgc cggattttga aacggcgcac ctgctctacc gcaaagcggc
     9901 ggcacaaggc ttgccggaag cacattggca actcgggctg caataccgtt tcggacaagg
     9961 gacgaaagcc gacacggcac aggccgtcaa tcatttgcgc gccgccgcgc aacaaggata
    10021 cattcccgcc tacaccccgc ttgccgaact catcctacct acggctcccg atgaagccgt
    10081 ttaccggttc caacaggcgg cacaggaaaa tgaccccgat gcccatgccg cactggccga
    10141 catctacctg caaggcaagt atctggaaag aaaccacaaa cttgccctgc atcatgccga
    10201 agcagccgcc gccgaacgcc atcccgaagg tttgcggata ccgggcgaca tctgccgcta
    10261 cggtttgggc atagccctcg atacggaaaa agcccggcat tattaccggc aggcagccga
    10321 agccggcagc cttaccgcct atcagaaact catatccgac agcgcgttaa accatcccga
    10381 gcaatatgac ggcatcaaag attccgccat taggcggcaa agggcagaac agctttatca
    10441 aaaagcccaa gccctgcatt acggattaca atgcgcgccc gaatacgcag ccgcgctcaa
    10501 actctacaca gaagcagcag aactcggaca cagcaaagcc caaaccaatc tgggcagtat
    10561 gtactacttc ggacagggca tagccgccga ctactgcaaa gcccgcaaat ggtttgaaca
    10621 agccgccgcg caaaaagaca gtatggcgtt ctacaacctc gcctgcatcc attacagcgg
    10681 acacggtgcc aggcggacaa agaaaaagcc tgccgctacc tgcaagaagc cataaacaac
    10741 ggatacgggc aaaaaagcgt cctgcaagaa ctgctgcaac agtggcaaaa tgccgtctga
    10801 acagagttac acctaccctg ccgaaacgaa acaggtataa tcgccccttt cccttcccgc
    10861 cgtccgaaca ggcctttcac attcagacgg catcctgatt gcacaagcgc acagaagcat
    10921 tatgacagac accgccgaaa accaaacacc aaacgaccgt caagccggac acccccgcag
    10981 catccgcagt ttcgtcctcc gccaaagcca catgaccgcc gcacagcaac gcgccatcga
    11041 taccttatgg gacagcttcg gcatcgacta ccaagcaaca ccggccgatc ttgatgcccg
    11101 tttcggaagc agccgaccta aaatcctcga aataggcttc ggtatgggaa tggcaagcgc
    11161 agaaatcgcc cgccgcctgc ccgaaaccga ctttctcgcc atcgacgtac acggccccgg
    11221 cgtaggcaac ctgctcaaac tcatcaacga aaaccatttg gaaaacatcc gcgtgatgcg
    11281 gcacgatgcc gtagaagttg tcaaaaatat gctgcaagac ggctcgctcg acggcatcca
    11341 catattcttc cccgacccgt ggcacaaaaa acgccaccac aaacgccgtc tgatacaagc
    11401 ccccttcatc gccaaactac tgcccaaact caaaaccggc ggctatatcc acctggcgac
    11461 agactgggaa gaatatgcac agcagatgct tgaagtcctc agcagcttcg acagcctgca
    11521 aaatacggcg gcagactacg cccccactcc ggactaccgc cccgaaacca aattcgaagc
    11581 gcgcggcaaa cgcctcggac acggcgtttg ggacttggta ttcaaacgga tcggataaca
    11641 gaccgccgga taaaaaaatg ccgtctgaag catgtttgct tacagacggc attctttcaa
    11701 gataaagcag cgggcgatgt ttcaatacaa gttttgaaac aatggtttga acggcaaaaa
    11761 cgcgtgtata ccgcacgcat ccttgtaggt tttaacctgc acatcggttt taaagtttat
    11821 tccgcccgca gatagtggca cgcggttggc ggagatgcag gctacggctt gctaattatt
    11881 catcgttacc aaaagaagac ttaggtgtac attcaggcat agttaacaaa acaactaaac
    11941 tt
//
LOCUS       DAXDSD010000072        11645 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXDSD010000072 DAXDSD010000000
VERSION     DAXDSD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11645
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.072"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(91..525)
                     /gene="queD"
                     /locus_tag="ACFB2Q_001533"
     CDS             complement(91..525)
                     /gene="queD"
                     /locus_tag="ACFB2Q_001533"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC0918532.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYCRLKNAGLNVCRVKLWETPTSCAEYEGGEGIF"
     gene            complement(613..1128)
                     /locus_tag="ACFB2Q_001534"
     CDS             complement(613..1128)
                     /locus_tag="ACFB2Q_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC0918533.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(1156..1815)
                     /gene="queC"
                     /locus_tag="ACFB2Q_001535"
     CDS             complement(1156..1815)
                     /gene="queC"
                     /locus_tag="ACFB2Q_001535"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC0918534.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYPDCRGVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(2000..2205)
                     /locus_tag="ACFB2Q_001536"
                     /pseudo
     CDS             complement(2000..2205)
                     /locus_tag="ACFB2Q_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2304..3530
                     /locus_tag="ACFB2Q_001537"
     CDS             2304..3530
                     /locus_tag="ACFB2Q_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC0918535.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALITA
                     FCLETARFLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGRTLSVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(3551..3832)
                     /locus_tag="ACFB2Q_001538"
     CDS             complement(3551..3832)
                     /locus_tag="ACFB2Q_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC0918536.1"
                     /translation="MIREEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEAASHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            3911..4027
                     /locus_tag="ACFB2Q_001539"
                     /pseudo
     CDS             3911..4027
                     /locus_tag="ACFB2Q_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4028..5746)
                     /locus_tag="ACFB2Q_001540"
                     /pseudo
     CDS             complement(4028..5746)
                     /locus_tag="ACFB2Q_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            complement(5848..6063)
                     /locus_tag="ACFB2Q_001541"
     CDS             complement(5848..6063)
                     /locus_tag="ACFB2Q_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918537.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(6077..6424)
                     /locus_tag="ACFB2Q_001542"
     CDS             complement(6077..6424)
                     /locus_tag="ACFB2Q_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918538.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(6609..7883)
                     /locus_tag="ACFB2Q_001543"
     CDS             complement(6609..7883)
                     /locus_tag="ACFB2Q_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC0918539.1"
                     /translation="MNRPKQPFFRPEVAIARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTMEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEEMLRKYRFLSANDAVSKQEMMNVEAELLEQKAKLDAYRREEA
                     GLLQEIRTQNLTLASLPKRHETEQSQLERTIADISQEVLDFEMRSEQIIRAGRSGYIA
                     IPNVEVGRQVDPSKLFLSIVPERTELYAHLYIPSSAAGFIKPKDKVVLRYQAYPYQKF
                     GLASGSVVSVAKTALGRQELSGLGMVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPL
                     QIGMTLEADILHEKRRLYEWVLEPIYSMSGRL"
     gene            complement(7944..8216)
                     /locus_tag="ACFB2Q_001544"
     CDS             complement(7944..8216)
                     /locus_tag="ACFB2Q_001544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918540.1"
                     /translation="MLPQPKEVPHGGILLQYLLLQRQFISCRKIFSVLYGANGVYNLT
                     RDWVNDAVNAPPYNGRPIFEIEHGLTAPATKADKSGNGYTDGTDYC"
     gene            complement(8291..8518)
                     /locus_tag="ACFB2Q_001545"
     CDS             complement(8291..8518)
                     /locus_tag="ACFB2Q_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918541.1"
                     /translation="MKNWKQFIFFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(8527..9681)
                     /locus_tag="ACFB2Q_001546"
     CDS             complement(8527..9681)
                     /locus_tag="ACFB2Q_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC0918542.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASCKYINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDARDMYAVQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
     gene            9933..10214
                     /locus_tag="ACFB2Q_001547"
     CDS             9933..10214
                     /locus_tag="ACFB2Q_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918543.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            10211..11254
                     /gene="argC"
                     /locus_tag="ACFB2Q_001548"
     CDS             10211..11254
                     /gene="argC"
                     /locus_tag="ACFB2Q_001548"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC0918544.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
BASE COUNT         2766 a         3027 c         2843 g         3009 t
ORIGIN      
        1 aatcgttctc tttgatctga ggcgaggcaa cgccgtaccg gtttaaagtt aatccaccat
       61 accttatttg aattgactat atcgatacgt tcaaaatatt ccctcccccc cttcatactc
      121 cgcgcacgat gtcggcgttt cccacaattt gacgcggcac acgttcagcc ccgcgttttt
      181 cagacggcag tacatttcga ccgccatatt ttcggcagtt gtgcggcagg gcaggcgcag
      241 ggttttcatg ttccagcctt ccaaaagcgc ggcgatttga gattcgcgcc cgttgccgcc
      301 gtggtagatg aaggcgtggt cgaaatggtc ggtaatgtgt tgtttcacaa tggctttcaa
      361 gtcggtaaag tccatcacca taccgtcttt cgctccgcct ttgatggtgc cgtctgaaac
      421 ggtgatttcg agtttgtagg tatgtccgtg caggttttgg catttgccgt catgcccgtc
      481 gagcatatgc gaggagtcga aggtaaatat cttggtaatt ttcatcccga ttccttttca
      541 gtgaaaaccc cgccgctttc cgcccggagg gtggcttgat gtgtcgtttt gcgtggaaac
      601 atatccgctg cttcaaatgg gcaaatagcc ttgaaagccg atttcggcgg gtttgccgtg
      661 cggattcggg gtcaggcgga tatggaaatt gccgatgtgc tcgagcgcaa gcaggttgtc
      721 cacgtctttc aagttgtctt tatgctgccg aacatagtcg gtcaacaggg tttgcagggc
      781 tttttgtttg gcttcggcag gcgtggcggc ggcgaacagc ccgaacgcgt gcgcctcggc
      841 aagctgcccc gcgcgatagc cgccgacatt ggcaaaatac aggcgcggag cgttttcagg
      901 cggcattttc gttttgggcg tttcggatac ggcgatgtcg taaccgtccg cccattcgac
      961 aacctgccaa ccgtcgatgt ggattttgtc cgtatcgccg aaccacgcgg ctttgagtgc
     1021 ggggacggct tcgcggtagt cgtcgcacac ggcaaattgg atgtcgtgca cttcgatatt
     1081 cgaccggccg gcattgccgc cgaggtaaaa catatggagc ttgggcatat cgtattcctt
     1141 gtgctttgcg cgcgttcaga cggccttttt actttccaga cattccgcca gcccgcgttc
     1201 gcgcaagata cagctcgggc attcgcggca gccgccgacg atacccttgt agcaggtgtg
     1261 ggtctgttcg cggatgtagt ccaacacgcc catttcgtcc gccaacgccc acgtttgcgc
     1321 cttggtcagg tacatcagcg gcgtgtggat ttgaaaatcg tagtccatcg ccagattaag
     1381 ggtaacgttc atggatttga cgaacacgcc gcggcagtcg ggatagcccg aaaaatcggt
     1441 ttcgcacacg cccgcgatga tgtgccggat accctgccct ttggcaaaaa tggcggcgta
     1501 aagcaggaaa agcgcgttac gcccgtccac aaaggtattg ggaacgccgt tgtcggcggt
     1561 ttcgatggcg gcggtttcgt ccatcagggc gttgtgcgta atctgccgca tcaggctcaa
     1621 atcgagtacg gtttgactga cacccaaatc ctgcgcgatc cactctgcgc gttccagctc
     1681 gacggcatgg cgttgcccgt atcggaaggt gatggcttgg acgttttcgc gcccgtaggt
     1741 ttggattgcc tgaatcaggc aggtggtcga atcctgaccg cccgagaaga tgaccaaggc
     1801 tttttggttt gacatatcga atcctagttt tggtaacgcg ggaggtttgc gaaccgcgaa
     1861 gttggacagt aaagacgcgg attatacagg caaacgcgtt cagacggcat ctgaacgcca
     1921 tgccgtctga acacgcggcg tatgccgact tgacggcgag ttccgccaag cgtctgcctt
     1981 gtgcgaaggc gatattgttt tcttcggcgg tcaggacggg agccgctgtc gggaatatct
     2041 ttatttgacg gcttcgcaga cggcggacac tttggggacg gtgcgcaata cggcttcgca
     2101 accttcaacg ctgtcgatgc cgcgagtgat tcggcgtgcg ggattgcggg tgctgccgtt
     2161 ttgggaatag tagaggacga ggattttgag gggatttggg ttcatgggcg gattccgtta
     2221 aaatagtttg gtgttcagac ggcataacgg ttgtccagat gccgtctgaa acagtttgaa
     2281 aacatgaaac ctggtaagag attatgacct ttttacaacg ttggcaaggt ttggcggaca
     2341 ataaaatctg tgcatttgca tggttcgtca tccgccgttt cagtgaagag cgcgtaccgc
     2401 aggcagcggc gagcatgacg tttacgacac tgctggcact cgtccccgta ctgaccgtaa
     2461 tggtcgcggt cgcttcgatt ttccccgtgt tcgaccgctg gtcggattcg ttcgtctcct
     2521 tcgtcaacca aaccattgtg ccgcagggcg cggatatggt gttcgactat atcgacgcat
     2581 tccgcgatca ggcaaaccgg ctgaccgcca tcggcagcgt gatgctggtc gtaacctcgc
     2641 tgatgctgat tcggacgata gacaatgcgt tcaaccgcat ctggcgggtt aacacgcaac
     2701 gcccctggat gatgcagttc ctcgtttatt gggcgttgct gactttcggg cctttgtctt
     2761 tgggtgtggg catttccttt atggtcgggt cggttcaaga ctccgtactc tcctccggag
     2821 cgcaacaatg ggcggacgcg ttgaagacgg cggcaaggct ggctttcatg acgcttttgc
     2881 tgtgggggct gtaccgcttc gtgcccaacc gcttcgtgcc cgcccggcag gcgtttgtcg
     2941 gagctttgat tacggcattc tgcctggaga cggcacgttt cctgttcacc tggtatatgg
     3001 gcaatttcga cggctaccgc tcgatttacg gcgcgtttgc cgccgtgccg tttttcctgc
     3061 tgtggttaaa cctgctgtgg acgctggtct tgggcggggc ggtgctgact tcgtcgctgt
     3121 cttattggca gggcgaggcc ttccgcaggg gattcgactc gcgcggacgg tttgacgacg
     3181 tgttgaaaat cctgctgctt ctggatgcgg cgcaaaaaga aggccgaacc ctgtccgttc
     3241 aggagttcag acggcatatc aatatgggtt acgatgaatt gggcgagctt ttggaaaagc
     3301 tggcgcggta cggctatatc tattccggca gacagggctg ggttttgaaa acgggggcgg
     3361 attcgattga gttgagcgaa ctcttcaagc tcttcgtgta ccgcccgttg cctgtggaaa
     3421 gggatcatgt gaaccaagct gtcgatgcgg taatgacgcc gtgtttgcag actttgaaca
     3481 tgacgctggc ggagtttgac gctcaggcga aaaaacagca gcagtcttga gtatttttga
     3541 aactgtattt ttatcctaaa gacattttcc tatctgctga ttccaccgtt tcctttctgt
     3601 gtgccgccat aatttttata atagacaagc cattcaagtt tgcattgact gctttttcat
     3661 tggcaatatc caaatgactg gccgcttcat ctaaaaatag gattttcggt tcgcaatata
     3721 aggcccgcgc caatacgata cgttgttttt gtccgcctga cagtgcgctg cccatatcgc
     3781 cgatcaaggt ttcatagccc ataggcatgg cggagatttc ttcgcgaatc atagcaaggc
     3841 aggcgcaatg ttcgattttt cctctatccg gttctgtatc gaaaaaacag atattctctg
     3901 caattgagcc agcaaatgca aatatagtgg attaaattta aatcaggaca aggcggcgaa
     3961 gccgcagaca gtacagatag tacggcaagg cgaggcaacg ccgtaccggt ttaaatttaa
     4021 tccactataa aacatcgtcc tgcctgacca tcgcactcaa attgcggata aaacgtggcg
     4081 gtaagctgta aatgtcgtgc ccatttatca tgactttgcc tgattcggga ggtagattgc
     4141 cgcttaaaat atccaaaaat gtcgatttac cgcagcccga tcgtccgatt aacgcaagcg
     4201 attcaccacg tccgatcttg aagctgacgt tttgtaaaac atatggctcg ttatcggcat
     4261 aacggaaaga aacattttcc actgagattt cgatgtcgtc tgaaatatca ggaatgccta
     4321 catccggcgt agcttccgat tctgtttcat tcaatacggt gtcagctaac cgttccgcat
     4381 gaagtgacag cattttaatt tgcacaaact gatcgaccag cgagccaata cgtccttcaa
     4441 actgtccctt ataagccaaa aaagccatta gtacgccgac ggtaaagacg ccgtccaata
     4501 ccatgcccgc agctaagtaa ataatcaaaa tatttgccaa accgaaaagc agcttgtttg
     4561 ccaagccgaa ccaaattccc agtttctgca ccgtcagtcc cgtattaacc gtatccacca
     4621 gcagccccat ccatgcagaa tatcgttgag gacctttacc aaattgcttg actgctcgga
     4681 taccgcgtac cgtttccata aaatagctgc tttgtttggc agcatggatg atgttttcct
     4741 ccgtcgcccg tcgcaacgga taataggcta cccaacggac tgcgatataa actaccagtg
     4801 tcgctgcaac caaacctgtc agtatcgggc tgtacactat cattagcaca aaagtaaaaa
     4861 cagccgttag gctgtttaaa accaacacga aaaacgttga agtcagagtt ccctgaatac
     4921 tatccaccgc cccgaagcgc gataccacat cgcccaaatg ccgtttagcg aaataatctg
     4981 taggcaaatc tatcaaacgt ttgaaaacgt ttgatttcca ctgaacattt aacgtcgttg
     5041 aaaaatgcat gcccaaccag ccctgcatga cagacaccgt attttgtacc agaagtagca
     5101 aggtaaagcc caaagccagc gtcgctaaca gattacgatc gcccgttacc actacatggt
     5161 cgatgaccca ttgcataaaa aacggcgaca ccaaagcaaa cacttccatt gctgctgcca
     5221 aagccaaaag ctgaaacaat gtccgcccca gcccagaaat cccgcgcaac atgggtagga
     5281 tgcggatttc ctgcttttct tcccctgcct cgaaacgcgt gtttggccac agttccaaag
     5341 caattcccgt aaacttgcgc gatatttcct ccgtcttgac tttgcgtcgt cccgaagccg
     5401 gatccatgac ggcagccccg tccgaagata ccgattccag caccacaaaa tgattcaaat
     5461 cccaatgtag aatacagggc aggcgcaaac tgcccaattc gtctaaatcc agcctcaacg
     5521 cccgtcccgt cagccccata tcatcagcaa aacgaacaat atctgccaaa ttctcaccct
     5581 taagtgacag acagtatttt gaacgcagtg cgcgcaaatc cgtatagaaa ccataaaatc
     5641 cggccacagc cgctagacat gccaagccac actccgctac ttctgtttgc agtataacgg
     5701 gcagcttttt tgtcaagccc aaagacaggt tttgcagata atccatagat ttccttgttg
     5761 aatcagaaaa gagagatggg aagacgggaa tggtttggaa aggccgtctg aaaaggtttc
     5821 agacggcctg cagccccata ttgactttca atgcgaccgg ttttcctcgc gtttcaaata
     5881 cagcagatac gcaagaaaag acaaagcccc tacaggtcct gcgacggtaa ccgaaaaaaa
     5941 cctgcctttg taaaacacac cgtcgaagtt taaaaaatag caaaggatgt cggtcaggat
     6001 gctgatggca agataaatca gcacagcggc tttccagtgt ttcaggtaat gtgcgaaatc
     6061 catttcaaac tcccgatcaa cagttgcacg ggtcgcaatg gcagtcttct ccgcattggc
     6121 agttgcgcct gtcgatcatt tttccccatt ttgcaccggc aaaaccgccg atatgcccac
     6181 ccaatgcaga accgccctca gctcctaccc atccgccgcc tgcgccgccg atggcaattc
     6241 cggcaggatt tccgcctgtt accaatgcgc cgcccgccat tcccgtgaat gctccggcaa
     6301 acgaccctaa agcgccgccg accttgcttc cgacccattc tccggagact ctgcccctgt
     6361 gcccccaagt accttctgtg ccgccgaaaa cagcggtcat ttcgttcatg gtcaattctc
     6421 tcatttcaaa actcctttta tcgtcaatcg aaaaattgaa agggcagata ccctatcctt
     6481 gcttttacag acagagtatc tgtaaaaggg ggtagtttca ggcagcttca gtgtttatgt
     6541 ttttatgtag tgttttacag gtcgtctgaa gaggtttcag acgacttttt atctttcagc
     6601 ctcaagcctt acaacctgcc cgacatacta taaatcggct ccaataccca ttcgtacagc
     6661 cgccgtttct cgtgtaggat gtctgcttcc aacgtcatgc cgatttgcag cggtttttcc
     6721 tcaccgtatg cagtgatggt tggtttgtcg ggttttattt tcacgagata aacaggttcg
     6781 ttgctcttcg ccaaatcgga ggataccatg cccaatcccg acaattcctg tctgcccagt
     6841 gccgtttttg ccactgatac gacactgccg gaagcaagcc cgaatttttg atagggatat
     6901 gcctgataac gcaggacaac cttgtctttc ggcttgataa agcctgctgc actgctgggg
     6961 atatatagat gggcatataa ctcggtacgt tcgggaacaa tgctcaagaa cagtttggaa
     7021 ggatcaacct gccgtccgac ttcgacgttc ggtattgcta tataacccga ccgtcctgca
     7081 cggatgattt gttcagagcg catttcaaaa tccaaaactt cttgagaaat atcggcaatg
     7141 gtgcgttcaa gctggctttg ttctgtctca tgccgtttgg ggaggctggc caatgtcaga
     7201 ttctgcgtgc ggatttcctg aagcagcccg gcttcttctc ggcggtaggc atcaagtttg
     7261 gctttctgct ctaaaagctc tgcctcgaca ttcatcattt cttgttttga cactgcatca
     7321 ttggcggata ggaaacgata tttccgcaac atttcttccg caagtctaat gcgccttttc
     7381 tggccgtcta tctgttgcga aatatggagt ttctggtttt ccaaacgttc gacagttgct
     7441 ttaaggctgc gcgtttcatt ctcgtgtatc agcttcagac gacccagttc ctgttctgcc
     7501 aacgttttct tcaaaactgc ctccgttttc aactgctgct gcacgctacc tcctgcgccg
     7561 aaacgtgagg tcgaaagcgc aaatagcttg tcgccagcct taaccttttc tccatcttcc
     7621 acgaatttcg ctgtaattgt ccccgtatcc ggtgcataca ccctgattac gcccgatgca
     7681 ggtagaattt gtccctccat tgttgtcttt cgcgtatagt taccgaatat caaaaacagg
     7741 ataatcagta acgcagatat cgatgcaaat gtcgtccaca gggaaaatga caacgggcgt
     7801 gtcagaatca ctttacccgt caggctggtt tggcgggcaa tggcgacttc agggcggaag
     7861 aaaggttgct tgggtctatt cataaaattg aagttaagaa agtttcagac gacccctaga
     7921 gatcgtctga acaatgagaa atatcagcag taatctgtac cgtcagtgta gccgtttcct
     7981 gatttatctg cttttgttgc gggagcagtt aatccatgtt caatctcaaa gattggtctt
     8041 ccgttataag gaggtgcatt aacggcatca tttacccaat cacgagtcag attgtataca
     8101 ccatttgcac catatagcac cgaaaaaatt tttcggcagg aaatgaactg tcgctgcagc
     8161 agcaggtatt gcaacaaaat ccccccatgt gggacctcct ttggttgtgg cagcattagc
     8221 tacatttcca gctatattgt ctgttacagg acctccccct gaaaccagct tcaattcatg
     8281 aagttgaagt tcaatcattt ttatactcct tttcttggtt ggtattccta aaaattcggc
     8341 taacaaaaac atatggcaga tatattgaaa aaaaattcaa agtaccctga ataaaattca
     8401 aattccaact atatttgtta atgtagtcga gaagaaacat atctgataaa gaatatagca
     8461 attgataaca agctattact aatattacga aaaatataaa ctgcttccag tttttcatag
     8521 aatccctcac aaaatttcca gaaaatctaa ctctatcaac tgatagatca acttcctaac
     8581 ttcttcatgt tttgcctgat tgaagttaac cagtagattt ttcaacaata acggttcatt
     8641 cttaccgatg tgttctaaca cttttttccc caactcatct acgcttatct tcatcccatt
     8701 cccaatcaaa tatccctttt ccaacgtatc caaattattg gcatttagtc tcaacctgac
     8761 gtcgtctgaa agcggagtag cgttgggatt cgcgaactgt tcgagatgaa aagcggtatc
     8821 ggtacgttct ttgccgagaa agtcttcact gaaagcttcg taattgacgg ggtcggcaat
     8881 catggcggca atttgtgcgg cagtatcgtt gatacgcgtc ctatcttgct cccagtctga
     8941 gaaactgtgg cgcagacttt ctatggtggg aaatttcttc attagccact cgaggtaatt
     9001 atagccgttt ggtgggaagg taccgacagc gaagtggaag gtttcacagc cgagcgggat
     9061 aggtctgtgc caccaaccgc gtgggatgta gaggacatca cctgcttcaa ggataatatc
     9121 catatcgata tgttcaggaa tggaaatatc agtatcttta gtctgttgca tatacaatgg
     9181 catagggaaa tcaggggcag taagttgcca acgtttcttg ccgaaaagct ggacggcata
     9241 catatctcgg gcgtcccaat ggtttttata agattcgtcg ctgccaaaag caagatatcc
     9301 actaacaata gtatgtgcgc cggcaaagcg ggcgacttga cgggcgatat ggtctgaaaa
     9361 cggctcgttg ttaatatggt tatagactaa cgacgcaccg ttcttcatat gttcgtagat
     9421 aacggattta ataaaacggt agcgagtttt gcccaaatcg tcgaaacttt cgacgtattc
     9481 ttctttagga acgattgcgc cttttttacg cagatgaaac agcggtgcgg ttgggtctgc
     9541 tcgttggtat atctcgttga tatatttaca agatgcggct tcgagattcc gaaccgctcc
     9601 tttaaagagc ttgggctttt gatacagata agtctgtcgg aacgttttag gactaatgcc
     9661 gaagtcgaga tggatgccca ttacttcccc ttactcagaa aatatttaaa atttataatg
     9721 ttacatatag ttacaaatat tagagttttt tgtgtgtgcg tcaaggaatt gttgacaatt
     9781 ttagttaaaa atttgtctcc aaacggaaaa aagcggtttg ttttttgttg ttaacatttt
     9841 tattgtaatt aaataaaagg tcgtctgaaa acggttttca gacgaccttt tgctataatc
     9901 gggcttcatc gccccgttcg gtttggaacc ttatgaaaac cctcgtcctc ctcctgcttt
     9961 ccttctccac gaccaccgct ttcgccgcat acggtttggg tttggggcag gcaccgaaat
    10021 atcctgccgg ctttcgcgcc tacggttatg tttattccgg acggcagggc tgggttttaa
    10081 aaacagaggc ggatgccatt aaattagaca cgcttttcaa acgctttgtg taccgtcctt
    10141 ccgccgccaa tcaaaacccc gtcggacagc gttcggacgg catacccgcc aaccacacaa
    10201 aggaaaaacc atgagcaaaa aaatcaaagc cggcattgtc ggcgcgacgg gctacacggg
    10261 cgtggaactg ctgcgcctgc ttgccgccca tccagatgtc gaagtcgccg ccgtaaccag
    10321 ccgcagcgaa gcgggaaccg cagttgccga ttactttcca agtctgcgcg gcgtgtacgg
    10381 cctcgccttc caaacgcccg acgaggcagg tttggaacaa tgcgacatcg tcttcttcgc
    10441 cacgcccaac ggcatcgcca tgaaagacgc gccgcgcctg attgaacagg gcgtgcgcgt
    10501 catcgacctt tccgccgact tccgcatacg ggacattccg acctgggaac actggtacgg
    10561 catgacccac gccgcccccg gcctcgtttc ccaagccgtg tacggattga gcgaactcaa
    10621 ccgcgaagcc gtcgcacagg cgcgcctcgt cgccaacccc ggctgctacc cgacctgcgt
    10681 atccctgccg ctcgtgccgc tgttgcggca atgccgtctg aagcccggta tgccgctgat
    10741 tgccgactgc aaatccggcg tgtccggcgc gggcaggaaa ggcaatgtcg gttcgctgtt
    10801 gtgcgaagcc ggcgacaact tcaaagccta cggcacagcc ggacaccgcc acctgcccga
    10861 aatcaggcag accatcgccg ggcttcagga cggcatcgcc gaaggattcg tgttcacgcc
    10921 gcacctcgcg ccgatgatac gcggtatgca cgccaccgtt tacctccacc tttcagacgg
    10981 cagcgacccc gaaaccgtcc tgcgcgacta ctaccgcgac agcccgttca tggacatcct
    11041 gcccgccggt tcgacacccg aaacccgcag cgtgcgcggc gcaaacctct gccgcatcag
    11101 catccgtcag gcggcgcaat ccgatgtgtg ggtcgtcctt tccgtcatcg acaacctcgt
    11161 caaaggcgcg gcgggacagg cggtgcaaaa tatgaacatt atgttcggac tggaggaaac
    11221 acacggcttg ggcggcatcc ccctgctccc ctgaagcgta aacggcaaac cgcaggcatc
    11281 gtgcctgcgg ttttttgatg ccgtctgaaa gcggcggttt ttttgggttc ggacggcttt
    11341 tggctcatca ttccgcgaaa acaaaaatct aaaataccgt cattcccgca aaagcgggaa
    11401 tctagtttat ccggcttcag caatttccga cacatttcca cacgcttcga ttccgtcatt
    11461 tctccggttt caatcgtttc cgataacacc gtggtttttc atttctagat tcccgcctgc
    11521 gcgggaatga cggcgggggg cttgccgttt tcccgataag cctctgcggc ttctcattgc
    11581 cggatttccg cccgcttggg aataacggga tttaaaatca tgaaaatgtg tcaaaaataa
    11641 tatag
//
LOCUS       DAXDSD010000073        11566 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXDSD010000073 DAXDSD010000000
VERSION     DAXDSD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.073"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..486
                     /gene="def"
                     /locus_tag="ACFB2Q_001549"
     CDS             <1..486
                     /gene="def"
                     /locus_tag="ACFB2Q_001549"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC0918545.1"
                     /translation="LQYPDERLHTVAKPVEQVDERIRKLVADMFETMYESRGIGLAAT
                     QVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDAVTRAERVK
                     VEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLKKRQKHT
                     I"
     gene            574..1500
                     /gene="fmt"
                     /locus_tag="ACFB2Q_001550"
     CDS             574..1500
                     /gene="fmt"
                     /locus_tag="ACFB2Q_001550"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC0918546.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            1579..2838
                     /gene="rsmB"
                     /locus_tag="ACFB2Q_001551"
     CDS             1579..2838
                     /gene="rsmB"
                     /locus_tag="ACFB2Q_001551"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC0918547.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARRQEALLDALWQALKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            2804..3403
                     /locus_tag="ACFB2Q_001552"
     CDS             2804..3403
                     /locus_tag="ACFB2Q_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC0918548.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            3403..5523
                     /locus_tag="ACFB2Q_001553"
     CDS             3403..5523
                     /locus_tag="ACFB2Q_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFC0918549.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDAQILTRSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            5516..6793
                     /locus_tag="ACFB2Q_001554"
     CDS             5516..6793
                     /locus_tag="ACFB2Q_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC0918550.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            6884..8071
                     /gene="dprA"
                     /locus_tag="ACFB2Q_001555"
     CDS             6884..8071
                     /gene="dprA"
                     /locus_tag="ACFB2Q_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC0918551.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            8097..8558
                     /locus_tag="ACFB2Q_001556"
     CDS             8097..8558
                     /locus_tag="ACFB2Q_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC0918552.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            8636..10942
                     /gene="topA"
                     /locus_tag="ACFB2Q_001557"
     CDS             8636..10942
                     /gene="topA"
                     /locus_tag="ACFB2Q_001557"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC0918553.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPGNGFAMKYQLISRNAKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPDEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDGKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELNETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLAYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIEPPAPQE"
     gene            11163..11291
                     /locus_tag="ACFB2Q_001558"
                     /pseudo
     CDS             11163..11291
                     /locus_tag="ACFB2Q_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            11281..11566
                     /locus_tag="ACFB2Q_001559"
                     /pseudo
     CDS             11281..11566
                     /locus_tag="ACFB2Q_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2812 a         3235 c         3318 g         2201 t
ORIGIN      
        1 ttgcaatatc ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca agttgacgag
       61 cgcatccgga agctggttgc cgatatgttt gaaacgatgt acgaatcgcg cggcatcggg
      121 ctggcggcga cgcaggtcga tgtgcacgaa cgcgtggtcg tgatggattt gaccgaagac
      181 cgcagcgaac cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg cgaaaccact
      241 tacgaagagg gctgcctgtc cgtaccgggc atttacgacg ccgttacccg cgccgaacgc
      301 gtcaaggtcg aggctttgaa cgaaaaaggc gaaaaattca cgctggaggc ggacgggctg
      361 ctggcgattt gcgtgcagca cgagttagat cacctgatgg gcatcgtgtt tgtcgaacgc
      421 ctttcccaac tcaagcaggg gcggattaag accaaactga aaaaacgtca gaaacatacg
      481 atttgattcg cttcgtcatg ccgtctgaac gctgcaaggc tttcagacgg cacagccttg
      541 tccgacaact ttacgcacac gcaggaacac gctatgaaag tcatctttgc cggcacgccc
      601 gattttgccg ccgccgcctt aaaagccgtt gccgccgccg gttttgaaat cccgctggtg
      661 ctgacccagc ccgaccgtcc gaaagggcgc ggtatgcaac tgaccgcccc gcctgtcaaa
      721 caagccgcgc tggaactcgg tttgcgcgtg gcacagcccg aaaaactgcg caacaacgcc
      781 gaagccctgc aaatgctcaa agaggtcgag gcggacgtaa tggtggttgc cgcgtacggc
      841 ttgattctgc cgcaggacgt gttggatacg ccgaaacacg gctgcctcaa tatccatgct
      901 tcgctgctgc cccgttggcg cggcgcggcg ccgattcagc gcgcgattga ggccggcgat
      961 gccgaaaccg gcgtgtgcat tatgcagatg gacatcggtt tggacaccgg cgatgtggtc
     1021 agcgaacacc gctacgccat ccaacctacc gatactgcca acgaagtcca cgacgcgctg
     1081 atggaaatcg gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag caaaggccgt
     1141 ctgaacgcgg tcaaacagcc cgaagaaggc gtaacttacg cccaaaaact aagcaaagaa
     1201 gaagcgcgta tcgattggag cgaaagcgcg gacattatcg aacgcaaaat ccgcgccttc
     1261 aaccccgtgc ctgccgcgtg ggtcgaatat cagggcaagc cgatgaaaat ccggcgtgcc
     1321 gaagtggtgg cgcaacaagg tacggcaggc gaagtgttgt cctgttcggc ggacggtttg
     1381 gtcgttgcct gcggcgaaag cgcgctgaag attaccgaat tgcagcctgc cggcggcagg
     1441 cgtatgaata ttgcggcgtt tgcggcggga cggtctattg aagcaggggc gaagctgtaa
     1501 atcccttcag acggcattcc gatccgcaaa cgggaatgcc gtctgaaacc atcagttgaa
     1561 gaaagcgaat cacataatat gagtatggca cttgcccaaa aacttgccgc cgacagcatt
     1621 gcggcggttg ccgaagggcg caaccttcag gacgtgttgg cgcacatccg cgcggcgcat
     1681 ccgcagctga cggcgcagga aaacggcgcg ttgcaggata tcgcctacgg ctgccagcgt
     1741 tatttgggca gtttgaaaca tatgctcgcg cagatgctga aaaagcccat cgacaatccg
     1801 cagctcgaaa gcctgctttt ggcggcgttg taccaactgc attacacgcg caacgcgccc
     1861 cacgccgtgg tcaacgaagc tgttgaaagc atagccaaaa tcgggcgcgg gcagtaccgt
     1921 tcgtttgcca acgcggtttt gcgccgcttt ttgcgcgaac gtgaaaaatt ggcggcttcc
     1981 tgcaaaaaag acgatgtggc gaaatacaac ctgccgctgt ggtgggttgc ctacctgaaa
     2041 aaccattatc cgaaacactg gcacaacatc gccgccgcgc tgcaatccca tccgccgatg
     2101 acgttgcgcg tcaaccgccg gcacggcaac gccgaaagct atttggaaaa actggctgtg
     2161 gaaggtatcg cggctaaggc gttggacgaa tatgcggtta cgttggaaga agccgtgccg
     2221 gtaaaccgtc tgcccggttt tgccgaagga ctggtgtcgg tacaggactt cggcgcgcag
     2281 caggcggcgt atctccttaa cccgaaagac ggcgaacgga ttttggacgc gtgcgccgcg
     2341 ccgggcggca agacggggca tattttggaa ctggcggact gccgcgttac cgccttggac
     2401 atcgatgcag gccgtctgaa gcgtgtggag gacaatatcg cccgtttggg ctttcagacg
     2461 gcatcggcgg cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg aaagcctttc
     2521 gacgcaatcc ttgccgacgt gccgtgcacc gcttccggcg tggcgcggcg caatcccgac
     2581 gtgaaatggc tgcgccgacc gaccgacgcg ctcaaaaccg cccgccggca ggaagccctg
     2641 ctggacgcac tttggcaggc gttgaaacag ggcggcagaa tgctgcttgc cacctgttcc
     2701 gtgttcgtcg aagaaaacga cgggcaactg caaaaattcc tcaaccgcca tgccgatgcc
     2761 gaaccgatcg aatcgcgggt gcttttaccg aacaaacacc aagatggctt ttattacgcg
     2821 cttattcaaa agcattaaac aatggcttgt gctgttgccg atgctctccg ttttgccgga
     2881 cgcggcggcg gagggcattg ccgcgacccg cgccgaagcg aggataaccg acggcgggcg
     2941 gctttccatc agcagccgct tccaaaccga gctgcccgac cagctccaac aggcgttgcg
     3001 ccggggcgta ccgctcaact ttaccttaag ctggcagctt tccgccccga caatcgcttc
     3061 ttatcggttt aaattggggc aactgattgg cgatgacgac aatattgact acaaactgag
     3121 tttccatccg ctgaccaacc gctaccgcgt taccgtcggc gcattttcca ccgattacga
     3181 cactttggat gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag tcctgaacaa
     3241 aggcgcgttg tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc tgacgctgtc
     3301 cacttcaaaa ctgcccaagc ctttccaaat caacgcattg acttctcaaa actggcattt
     3361 ggattcgggt tggaaacctc taaacatcat cgggaacaaa taatgcgccg cttcctaccg
     3421 atcgcagcca tatgcgccgt cgtcctgctg tacggattga cggcggcaac cggcagcacc
     3481 agttcgctgg cggattattt ctggtggata gtctcgttca gcgcaatgct gctgctggtg
     3541 ttgtccgccg ttttggcacg ttatgtcata ttgctgttga aagacaggcg caacggcgtg
     3601 ttcggttcgc agattgccaa acgcctttcc gggatgttca cgctggtcgc cgtactgccc
     3661 ggcttgttcc tgttcggcat ttccgcgcag tttatcaacg gcacgattaa ttcgtggttc
     3721 ggcaacgaca cccacgaagc cctcgaacgc agccttaatt tgagcaagtc cgcactggat
     3781 ttggcggcag acaatgccgt cagcaacgcc gttcccgtac agatagacct catcggcacc
     3841 gcctccctgt cgggcaatat gggcagtgtg ctggaacact acgccggcag cggttttgcc
     3901 cagcttgccc tgtacaatgc cgcaagcggg aaaatcgaaa aaagcatcaa tccgcaccaa
     3961 ttcgaccagc cgcttcccga caaagaacat tgggaacaga ttcagcagac cggttcggtt
     4021 cggagtttgg aaagcatagg cggcgtattg tacgcgcagg gatggttgtc ggcaggtacg
     4081 cacaacgggc gcgattacgc gctgttcttc cgccagccga ttcccgaaaa tgtggcacag
     4141 gatgccgttc tgattgaaaa ggcgcgggcg aaatatgccg aattgagtta cagcaaaaaa
     4201 ggtttgcaga ccttttttct ggtaaccctg ctgattgcct cgctgctgtc gatttttctt
     4261 gcgctggtaa tggcactgta ttttgcccgc cgtttcgtcg aacccattct gtcgcttgcc
     4321 gagggcgcaa aggcggtggc gcagggtgat ttcagccaga cgcgccccgt attgcgcaac
     4381 gacgagttcg gacgtttgac caagctgttc aaccatatga ccgagcagct ttccatcgcc
     4441 aaagaagcag acgaacgcaa ccgccggcgc gaggaagccg cccgccacta cctcgagtgc
     4501 gtgttggatg ggttgactac cggtgtggtg gtgtttgacg aaaaaggccg tttgaaaacc
     4561 ttcaacaagg cggcggaaca gattttgggg atgccgctcg cccccctgtg gggcagcagc
     4621 cggcacggtt ggcacggcgt ttcggcacag cagtccctgc ttgccgaagt gtttgccgcc
     4681 atcggtgcgg cggcaggtac ggacaaaccg gtccaggtgg aatatgccgc gccggacgat
     4741 gccaaaatcc tgctgggcaa ggcgacggta ttgcccgaag acaacggcaa cggcgtggtg
     4801 atggtgattg acgacatcac cgtgctgata cgcgcgcaaa aagaagccgc gtggggtgaa
     4861 gtggcgaagc ggctggcaca cgaaatccgc aatccgctca cgcccatcca gctttccgcc
     4921 gaacggctgg cgtggaaatt gggcgggaag ctggacgatc aggacgcgca aatcctgacg
     4981 cgttcgaccg acaccatcat caaacaggtg gcggcgttaa aagaaatggt cgaggcattc
     5041 cgcaattacg cgcgcgcccc ttcgctcaaa ctggaaaatc aggatttgaa cgccttaatc
     5101 ggcgatgttt tggccctgta cgaagccggc ccgtgccggt ttgaggcgga acttgccggc
     5161 gaaccgctga tgatggcggc ggatacgacc gccatgcggc aggtgttgca caatattttc
     5221 aaaaatgccg ccgaagcggc ggaagaagcc gatatgcccg aagtcagggt aaaatcggaa
     5281 acggggcagg acggacggat tgtcctgacg gtttgcgaca acggcaaggg attcggcaag
     5341 gaaatgctgc acaatgcttt cgagccgtat gtgacggata agccggcggg aacgggactg
     5401 ggtctgcctg tagtgaaaaa aatcattgga gaacacggcg gccgcatcag cctgagcaat
     5461 caggatgcgg gtggggcgtg tgtcagaatc atcttgccaa aaacggtaga aacttatgcg
     5521 tagcagcgat attttaattg tagacgacga agtcggcatc cgcgacctgc tgtcggaaat
     5581 cctgcaggac gaaggctatt cggtcgcgtt ggcggaaaat gccgaagagg cgcgcaagct
     5641 gcgccatcag gcgcgcccgg caatggtgct gctggatatt tggatgcccg attgcgacgg
     5701 tatcaccctt ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg tggtggtgat
     5761 gagcgggcat gccagcatcg ataccgccgt agaagcgacc aaaatcggcg cgatcgattt
     5821 tttggaaaaa ccgatttccc tgcaaaagct gctgtctgcg gtcgaaaacg cgttgaaaca
     5881 cggtgcggcg caaatcgaaa cgggaccggt gttcgacaaa ctgggcaaca gtgccgcgat
     5941 tcaagagatg aaccgcgaag tcgaagcggc ggcaaagcgc acgtcccctg tatttttgac
     6001 gggcgaggcg ggttcgccgt ttgaaaccgt ggcgcgctat ttccataaaa acggtacgcc
     6061 gtgggtcagt ccggcaaggg tcgaatatct gatcgatatg ccgatggaac tgttgcagaa
     6121 ggcggagggc ggcgttttgt atgtcggcga catcgcccaa tacagccgca atatccaaac
     6181 cggcattacc tttatcatcg gcaaggcaga acgctgccgt gtcagggtga ttgcttcgtg
     6241 cagctacgcg gcaggttcag acggcatttc atgtgaagaa aaattggccg ggcttttttc
     6301 ggaatcggtc gtccgtattc cgcccctgag tatgcagcat gaagacattc ccttcctgat
     6361 acaggggatt gcctgcaatg tggcggaaag ccaaaaaatc acgcctaccg cattcagtga
     6421 cgatgcgctt gccgtattgt cccgttacga ctggccgggg aatttcgagc agttgcaaaa
     6481 cgtcgtgaca acgctgctgc tggaggcgga cgggcaggaa atcggtatcg gcgcggcttc
     6541 ttcggcactg gggcgcggtg tgcctgccga cggaacgggg catatggcgt gcgggttcga
     6601 ctttaacctg ccgctgcgcg aattaaggga ggaggtggag cggcgttatt tcgagtacca
     6661 catcgcccaa gaaggtcaga atatgagcaa agtggcgcag aaggtcggtt tggaacgcac
     6721 gcacctttac cgcaaactca aacagctcgg catcggcgtt tcgcgccggg cgggggaaaa
     6781 aaccgaagaa taggcccgga cggccggttt cccggctgcg ggcttttgtt ttcagacggc
     6841 atttggagaa aatgccgtct ggaatcgtaa ggggatggat tttatgacag aggacgaacg
     6901 tttcgcgtgg ctgcaattgg cgtttacgcc ctatatcggc gcggaaagtt tcctgctgct
     6961 gatgcgcagt ttcggcagcg cgcaaaatgc cttgtccgca ccggcggaac aggttgcccc
     7021 ggcggtgcgg cataagcacg cgcttgaggc ttggcgcaat gcggaaaaac gcgctttggc
     7081 gcggcaggcg gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc gcctgatgct
     7141 gcttcaggat gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc cgccggtttt
     7201 gtttttgcgc ggcaacgtgc ggctgctgca caagccttcc gccgccatcg tcggcagccg
     7261 ccatgccacg ccgcaggcga tgcggattgc caaagatttc ggcagggcgt tgggtgggaa
     7321 aggcattcct accgtgtcgg gtatggcttc gggcatcgac accgccgccc atcagggcgc
     7381 actggaggca gaaggcggca ccatcgccgt gtgggggacg ggcatagacc gcatttatcc
     7441 gccggccaac aaaaaccttg cctatgaaat cgccgaaaaa gggctgattg tcagcgagtt
     7501 ccccatcggc acgcggccgt atgccggcaa ctttccgcgc cgcaaccgcc tgattgccgc
     7561 gctgtcgcag gttacgctgg tggtcgaggc cgccttggaa tccggttcgc tgattaccgc
     7621 cggcctggcg gcagaaatgg ggcgcgaagt gatggcggta cccggttcga tagacaaccc
     7681 ccacagcaaa ggctgccaca aactgattaa agacggagcg aaactgacgg aatgtttgga
     7741 cgacatcctg aacgaatgcc cggggctatt gcaaaatacg ggtgcttcgt catattctat
     7801 aaataaggat acgcccgaca cgggccgccg cgccgttcag acggcatacg ccccgccgcc
     7861 tgccgcaaaa atgccgtctg aaggggcggc aggcggcaca gccgtcggcg gcatactgga
     7921 taaaatgggt ttcgacccca tccatcccga cgtgcttgcc ggacagttgg ctatgcctgc
     7981 cgcagatttg tatgccgcac tgttggaatt ggaattggac ggcagcgttg ccgcaatgcc
     8041 cggcggcaga taccagcgta tccgaacttg aacgcacttt atattaagga acacgaatga
     8101 ccgaagtcat cgcctacctc atcgaacatt tccaagattt cgatacctgc ccgccgcccg
     8161 aagacttggg tatgctgctt gaagaagcgg gtttcgatac gatggaaatc ggcaacaccc
     8221 tgatgatgat ggaagtattg ctcaacagct ccgaattttc cgccgaaccc gccggcagcg
     8281 gcgcattgcg cgtgtacagc aaagaagaaa ccgacaacct gccgcaggaa gtgatggggc
     8341 tgatgcagta tctgattgaa gaaaaagccg tcagctgcga acagcgggaa atcatcatcc
     8401 acgcgctcat gcacatcccc ggcgacgaaa ttaccgtaga taccgccaaa gtgctgaccc
     8461 tgctgctttt atgggcaaac aagagcgagc tgcccgtatt ggtcggcgac gagctgatga
     8521 gcgcgctttt actcgacaac aaacccacga tgaactgaag cggcttcaga cggcgccggc
     8581 ccgcccgatg ccgtctgaaa caccggcgtc aaaaccacca tccagagaac gacaaatggc
     8641 gaaaaacctc ttaatcgtcg aatccccgtc caaagccaaa accctgaaaa aatatttggg
     8701 cggcgatttt gaaatcctcg cgtcctacgg acacgtccgc gacctcgtcc ccaaaagcgg
     8761 cgcggtcgat cccggcaacg gctttgcgat gaaataccaa ctcatcagcc gcaacgccaa
     8821 acacgtcgat gccatcgtcg ccggagccaa agaagccgaa aacatctacc tcgcaaccga
     8881 cccggacagg gaaggcgaag ccatttcctg gcatcttttg gaaatcctca aatccaaacg
     8941 cggcctgaaa aacatcaagc cgcagcgcgt cgtgttccac gaaatcacca aaaacgccgt
     9001 gctcgatgcg gttgcccatc ctcgcgaaat cgaaatggat ttggtcgatg cgcaacaagc
     9061 ccgtcgcgct ctggactatc tggtcggttt caacctctcg ccgttgctgt ggaaaaaaat
     9121 ccgacgcggt ttgagcgcgg gccgcgtgca aagccctgct ttgcgcctga tttgcgaacg
     9181 cgaaaacgaa atccgcgcgt ttgaagcgca ggaatattgg acggtacatc tagacagcca
     9241 caaaggccgc agcaagttca ccgccaaact cgcccaatac aacggcgcga aactcgaaca
     9301 attcgacctg ccggacgaag ccgctcaagc cgacgtgttg aaagaactcg aaggcaaaga
     9361 ggctgtcgtt actgccattg aaaagaaaaa gcgcagccgc aaccccgccg cgccgttcac
     9421 cacatccacc atgcagcagg atgccgtgcg caaactcggc ttcaccaccg accgcaccat
     9481 gcgtaccgcc cagcagcttt acgaaggtat agacgtaggg cagggcgcca tcggtctgat
     9541 tacctatatg cgtaccgaca gcgtgaattt ggctgacgaa gcgttaaccg aaatccgcca
     9601 ttacattgaa aacaaaatcg gcaaagaata tctgccgagt gccgccaaac aatacaaaac
     9661 caaatccaaa aacgcccaag aagcccatga agcgatccgc ccgacttccg tgtaccgcac
     9721 gcccgaaagc gtcaaaccct tcctgagtgc agaccagttc aaactctatc aaatgatttg
     9781 gcagcgtacc gttgcctgtc agatgacgcc cgccaaattc gaccaaacca ccgtcgatat
     9841 taccgtcggc aaaggcgtgt tccgagtaac cggacaagtg caaaccttcg caggcttcct
     9901 cagcgtttac gaagaaagca gcgacgatga agagagcgaa gacggcaaaa aactgcccga
     9961 aatgagcgaa ggcgacaaat tgccggtcga caaactctac ggcgaacaac acttcaccac
    10021 cccgccgccg cgctacaacg aagccacact ggttaaagcc cttgaagaat acggcatcgg
    10081 ccgcccctcg acctacgcca gcatcatctc cacgctcaaa gaccgcgaat acgttaccct
    10141 tgagcaaaaa cgcttcatgc ccaccgacac aggcgacatc gtcaataaat tcctgaccga
    10201 acacttcgcc caatacgtcg attaccactt caccgccaaa ctcgaagacc agcttgacga
    10261 aatcgccgac ggcaaacgcc gctggattcc cgtgatggac aaattctgga aaccgttcat
    10321 caaacaagtg gaagaaaaag agggcatcga acgcgccaaa tttaccacgc aggaacttaa
    10381 cgaaacctgc ccgaaatgcg gcgaacacaa actgcaaatc aagttcggca aaatgggccg
    10441 cttcgtcgcc tgcgccggct atcccgaatg cagctacacg cgcaacgtca acgaaaccgc
    10501 cgaagaagcc gccgagcgca tcgccaaagc cgaggctgaa caagccgaac tcgacggacg
    10561 cgaatgcccc aaatgcggcg gtcggttagc gtataaatac agccgtaccg gcagcaaatt
    10621 catcggttgc gccaactacc ccaaatgcaa acacgtcgag ccgttggaaa aacccaaaga
    10681 taccggcgtc caatgcccgc aatgcaaaaa aggcaacctc gtcgagcgca aatcccgcta
    10741 cggcaaactg ttttacagtt gcagcaccta tcccgactgc aactacgcca cttggaaccc
    10801 gcccgttgcc gaagaatgcc cgaactgcca ttggccggtc ttgaccatca aaaccactaa
    10861 acgccggggc gtggaaaaag tctgtccgca aaaagaatgc ggctggaaag aacaaatcga
    10921 accgcctgca ccgcaagagt gaagcggttg ggattgagat gaaagaaaat gccgtctgaa
    10981 aggggtttca gacggcattt gtaaattaga aggggctgtc ccagatggct ggggaaattt
    11041 aaattaagtc agaattatcc ctatgagaaa agccgtccaa gccggtacaa acaaaagaaa
    11101 cccatcggac tgtttgccgc aggcgtaact gcaagaacgg cagcagggtt ggcagcagtt
    11161 aatcaaaaca cggcagcctg ttattttcta tggcgaaata gtgtttggca catcatcggg
    11221 aaaacttcct ttacacgcat ttggacgaaa tctgcgaaat tatggaggca tacgacatat
    11281 cgtttacgat tacttatccg tcaaaacagt ccgcatttgg aaatgtatga cggcgaagca
    11341 ggggcgggtg aaagttattt ccgtaaaggc agacgcggcc gcagtgccgc cggcaaagcc
    11401 gccgtattcg ggcttttgaa gcgcaacggc aaggtttgta cggttactgt cggacactcg
    11461 aaccgctatt ttattgccta ttatccgcgg acgggtgaaa cccggcagca ttgtttatac
    11521 ggattgttcg tagttacgat gtatcagatt gtaagcggat ttggcc
//
LOCUS       DAXDSD010000074        11004 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXDSD010000074 DAXDSD010000000
VERSION     DAXDSD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11004
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.074"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..522
                     /locus_tag="ACFB2Q_001560"
     CDS             <1..522
                     /locus_tag="ACFB2Q_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HFC0918554.1"
                     /translation="PRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGILY
                     GEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKNK
                     LDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQLA
                     ALEAAQNETAASL"
     gene            660..911
                     /locus_tag="ACFB2Q_001561"
     CDS             660..911
                     /locus_tag="ACFB2Q_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC0918555.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            938..1156
                     /locus_tag="ACFB2Q_001562"
                     /pseudo
     CDS             938..1156
                     /locus_tag="ACFB2Q_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1307..1498
                     /locus_tag="ACFB2Q_001563"
                     /pseudo
     CDS             1307..1498
                     /locus_tag="ACFB2Q_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1528..1725
                     /locus_tag="ACFB2Q_001564"
     CDS             1528..1725
                     /locus_tag="ACFB2Q_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918556.1"
                     /translation="MPSEAAWAFRRHFLRLKQRIGNPAPPVHGNPHLKTICMPNHTPI
                     PVSVICTLPVLGNHRFFAYNS"
     gene            1758..2720
                     /locus_tag="ACFB2Q_001565"
     CDS             1758..2720
                     /locus_tag="ACFB2Q_001565"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC0918557.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            2767..3138
                     /locus_tag="ACFB2Q_001566"
     CDS             2767..3138
                     /locus_tag="ACFB2Q_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC0918558.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            3164..4090
                     /gene="fabD"
                     /locus_tag="ACFB2Q_001567"
     CDS             3164..4090
                     /gene="fabD"
                     /locus_tag="ACFB2Q_001567"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC0918559.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAEA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRTMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            4229..6094
                     /gene="msbA"
                     /locus_tag="ACFB2Q_001568"
     CDS             4229..6094
                     /gene="msbA"
                     /locus_tag="ACFB2Q_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC0918560.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAGIISTLQNWREQFTYMVWGTENKIWTVPL
                     FLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLMN
                     MLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRYY
                     RDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSKK
                     ITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKSL
                     ANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKAL
                     DNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLRA
                     QFALVSQDVFLFDDTLFENVRYSRPDAGESEVLSALQAANLQSLIDASPLGLHQPIGS
                     NGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGII
                     VAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            6194..7759
                     /gene="guaA"
                     /locus_tag="ACFB2Q_001569"
     CDS             6194..7759
                     /gene="guaA"
                     /locus_tag="ACFB2Q_001569"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC0918561.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            7821..8567
                     /gene="fabG"
                     /locus_tag="ACFB2Q_001570"
     CDS             7821..8567
                     /gene="fabG"
                     /locus_tag="ACFB2Q_001570"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC0918562.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKIIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            8632..9117
                     /locus_tag="ACFB2Q_001571"
     CDS             8632..9117
                     /locus_tag="ACFB2Q_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC0918563.1"
                     /translation="MNTHHHLPCPCGSGTSYAGCCRPLHLRQILPPTAEALMRSRYGA
                     YVLHLIDYIIATTVPAQQTFLDAAELMQWSREAEWLGLNVIAHRNLGKQHAQVEFEAY
                     FQDGGNRTVHHELSAFVKIAEQWYFIDPTVPLPTMKQPCICGSGKKFKACCGKYLQPV
                     A"
     gene            complement(9337..10131)
                     /locus_tag="ACFB2Q_001572"
     CDS             complement(9337..10131)
                     /locus_tag="ACFB2Q_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC0918564.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            10235..10960
                     /locus_tag="ACFB2Q_001573"
     CDS             10235..10960
                     /locus_tag="ACFB2Q_001573"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC0918565.1"
                     /translation="MPRFYLPENLSVGQTVDLPDNIVRHLNVLRIRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTRLIMSINRACKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
BASE COUNT         2766 a         3416 c         2622 g         2200 t
ORIGIN      
        1 ccgcgcgtcg ggctggtcaa cgtcggcaca gaagacatca aaggtacgga taccgtcaaa
       61 caaacctaca aactgctgca aaacagcaaa ctcaacttta tcggcaacat cgaaagcaac
      121 ggcatcctgt acggcgaagc agatgtcgtc gtcgccgacg gctttgtcgg caacgtcatg
      181 ctcaaaacca tcgaaggcgc agtcaaattc atgagcggag ccatccgccg cgaattccag
      241 agcaacctgt tcaacaaact tgccgccgtt gccgccctac ccgccctcaa aggcttgaaa
      301 aacaaactcg atccgcgcaa attcaacggg gccatcctgc tcgggctgcg cggcatcgtc
      361 atcaaaagcc acggcggcac agacaaaacc ggtttccgct acgcactcga agaagcctac
      421 cacgaagcca agtccgccgg cctttccaaa atcgaacaag gcgttgccga acaactcgcc
      481 gcactggaag ccgcccaaaa cgaaaccgcc gccagtctgt aacacgcacg atgccgtctg
      541 aacgcccccg cccctttcag acggcatccg gcacccgtcc aaacctgcgg gcgcggacgg
      601 cgatgcgcct gtccggcact tcccaaatac ttccctgtaa aataaggagt atttgaaaaa
      661 tgaagacatt agaaaaacgg atgaaagctc tagacaaacg gatgatgaag ttcggaaaat
      721 cccttgaagg caggcttgat gcccgtctga ttgaatccgc attggattat attcattatt
      781 cggaacgttt tttggctttt gacatcctgt gtacttatat cgaagatttc gatatccggc
      841 tgacggaaca agaatcccgg gaaatttctt ttatcaacaa ggaatttgag atagaaagca
      901 cgtccgatta accaataaag ccaatgattt gataagaatg aaaacatcga cggtcgtttt
      961 tggcggattt tttatggcag acaacgggga gcgaatccaa atccccgttt tggaaaatcc
     1021 tgacattagg gaaatcaatc gctttttttc cgtatcaaat tttgagaaaa aagccggcgt
     1081 ccttgttttc agaatcatcc ccgagccgga atttggcaat accgaattaa ctgtctattt
     1141 tgaaaaagga tattatagtg gattaacaaa aaccggtacg gcgttgcccc gccttggctc
     1201 aaagggaacg attccctaag gtgcccaagc accaagtgaa ccggttccgt actatccgta
     1261 ctgtccgcgg cttcgccgcc ttgtcctgat ttttgttaat ccactatatt tgccaataat
     1321 tcaaacaatt ttagaaggcg gggatattaa agtaaaaaac cttaaaacag aaaattacag
     1381 cgggaaggca agggaaatct tgggagattg ctaccctatt gaacatatct ctaaaaatat
     1441 ttctacaatt caaaatatca tttccgaatt tatcagacaa aaacaaacac cggcaccaaa
     1501 taatttgatg gaaacccaac aatcaagatg ccgtctgaag cagcttgggc tttcagacgg
     1561 catttccttc gcttaaaaca gcgtatcggc aaccccgccc cgcccgtcca cggcaatccg
     1621 catctgaaaa ccatctgtat gccaaaccac acccccatcc ctgtttccgt catttgcacc
     1681 ctgcccgtat tgggcaatca tcggtttttc gcttacaata gctgaatctg aaccaactct
     1741 ctaaaaaggc cgttcccatg cagtatgcaa aaatttccgg cacgggcagc tatcttcctg
     1801 ccaaccgcgt cagcaatgac gaccttgccc aaaaggtaga tacctctgac gagtggatta
     1861 ccgcgcgtac gggcatcaaa ttccgccata ttgcagccga aaacgaaaaa accagcgatc
     1921 ttgcagccga agcggcgcgg cgcgctttgg cagatgccaa attaaatata aacgacattg
     1981 atttgattat cgtggcgacg gcaacgccgg atatgcagtt tccttctacc gccaccatcg
     2041 tgcagcaaaa attgggcatc accaacggct gccccgcgtt tgacgtgcag gcggtgtgcg
     2101 caggctttat gtacgcgctg actacggcaa atgcctacat taaaagcggt atggcgaaaa
     2161 acgcgctggt catcggcgcg gaaaccttca gccgcatcgt cgattggaac gaccgcacaa
     2221 cctgcgtgct gttcggcgac ggcgcgggcg cggtggtttt aagcgcgtcc gacaaaccgg
     2281 gcatcatcca cagcaaactc aaagccgacg gcaattatct gaaactctta aacgtccccg
     2341 ggcaaatcgc ctgcggcaaa gtttccggtt cgccgtacat ttcgatggac ggtcccggcg
     2401 tgttcaagtt tgccgtcaaa atgctgtcca aaatcgccga tgacgttatc gaagaagcag
     2461 gttacaccgc cgaacaaatc gactggattg ttccccatca ggcaaaccgc cgcattatcg
     2521 aatcgaccgc gaaacatttg ggtttgagta tggacaaagt cgtcctgacc gtccaagacc
     2581 acggcaacac gtccgccgca tccattccgc tggctttgga cgcgggcatc cgcagcggac
     2641 aaatcaaacg cggtcaaaac ctgctgctcg aaggcatagg cggcggtttc gcgtggggcg
     2701 cggtgctgtt gcaatattga aaccggtgcc gtctgaaaca ggctttcaga cggcatttcc
     2761 catatcatga agcggcaggc tttcttcaaa ccgatggcgt gtgcggcatt tctgtccgcc
     2821 gtttcgctgc gcctccccgt attgggcgcg tgttacgcaa tattgtccct ctatgcgttt
     2881 gcactttacg gcatcgacaa acggcgtgcc gtgcggggaa aacgccgcat tcccgaacac
     2941 cgcctgctcc tgcctgcctt gttcggcggt tggacgggcg catacttggg tagtaggatg
     3001 ttcaggcata aaacggcgaa aaagcgtttt gttgtgctgt tccgtctgac tgtttcgggc
     3061 aatgtcctgg cgacctgcat cctgattgat tatttcgttc cgcccgaact ttttgtaaaa
     3121 ctcgggcaac atctctgaca atccaaccca acaaaggaat actatgtctt tcgcattctt
     3181 cttccccgga caaggttccc aaagcctcgg catgatgaac ggctttgccg aacacgccat
     3241 cgtcaaaaac acatttgacg aagcctccgc catattgggg caggacttgt gggcgatgat
     3301 taacggcagc gatgccgaaa tcatcggtca aacagtcaac acccagccca ttatgctcgc
     3361 cgccggcgtt gccgtttacc gcgcctattt ggaagtgggc ggcaaaacgc ctgccgccgt
     3421 tgccggacac agcctcggcg aatacaccgc gcttgtcgcc gccgaagcat tggattttgc
     3481 cgatgccgtc aaactcgtcc gcctgcgcgc cgaactgatg cagtccgccg taccgcaagg
     3541 cgtaggcgca atggcggcca ttttgggttt ggaagatgaa caggtcaggc agatttgtgc
     3601 cgaatccgca cagggcgaag tggtcgaagc cgtcaacttc aactcgcccg gacaagtcgt
     3661 gattgcaggc aacgccgccg ccgtcggacg caccatggct gccgccaaag aagccggagc
     3721 caaacgcgcc ctgccgctgc cggtatccgt tccttcccat tgcagcctga tgaaacccgc
     3781 cgccgacaaa cttgccgaag cactcaaaac cgttgaaatc aagcagccgc aaatccgtgt
     3841 catccacaac gccgacgttg ccgcctacga tgatgccggc aaaatcaaag acgcgctcgt
     3901 ccgccagctt tacagccccg tgcgctggac ggaaaccgtc aacgcccttg tttcagacgg
     3961 cattgccgaa tccgccgaat gcggtccggg caaagtgttg gcgggcttgg caaaacgcat
     4021 caacaaggcc gccgcttgca gcgcactgac cgatgccgga cagattaccg cctttatcga
     4081 agcgcactga cttcgttccg caaaaagcag cctgccctct tcaggctgct tttcacgtcc
     4141 gaacggcaaa cagccgcata tttgcgctat aattcatccc taccaaacca ccgacagcgg
     4201 ctgccgttgc agttcccgcc ctaccgatat gatagaaaaa ctgactttcg gactgtttaa
     4261 aaaagaagac gcgcgcagct ttatgcgcct gatggcgtac gtccgcccct acaaaatccg
     4321 catcgttgcc gccctgattg ccattttcgg cgttgccgcc accgaaagct acctcgccgc
     4381 cttcatcgcc cccctgatca accacggctt ttccgcaccc gccgcgccgc ccgagctgtc
     4441 cgccgccggc atcatttcca ccctgcaaaa ctggcgcgaa cagtttacct atatggtttg
     4501 ggggacggaa aacaaaatct ggaccgtccc cctcttcctc atcatcctcg tcgtcatccg
     4561 tggcatctgc cgctttacca gcacctatct gatgacttgg gtctccgtga tgaccatcag
     4621 caaaatccgc aaagatatgt ttgccaaaat gctgaccctt tcctcccgct accatcagga
     4681 aacgccgtcc ggcaccgtac tgatgaatat gctcaacctg accgaacagt cggtcagcaa
     4741 cgccagcgac atcttcaccg tcctcacgcg cgacacgatg atcgttaccg gcctgaccat
     4801 cgtcctgctt tacctcaact ggcagctcag cctcatcgtc gtcctgatgt tccccctgct
     4861 ctccctgctc tcgcgctact accgcgaccg tctgaaacac gtcatttccg actcgcaaaa
     4921 aagcataggc acgatgaaca acgtgattgc cgaaacccat cagggacacc gcgtcgtcaa
     4981 gctgttcaac gggcaggcgc aggcggcaaa ccggttcgac gcggtcaacc gcaccatcgt
     5041 ccgcctcagc aaaaaaatca cgcaggcaac ggcggcacat tccccgttca gcgaactgat
     5101 tgcctccatc gccctcgccg tcgtcatctt catcgccctg tggcaaagcc aaaacggcta
     5161 caccaccatc ggcgaattta tggcattcat cgtcgcgatg ctgcaaatgt acgcccccat
     5221 caaaagcctt gccaacatca gcatccctat gcagacgatg ttcctcgccg ccgacggcgt
     5281 atgtgcattt ctcgacaccc cgcccgaaca ggacaaaggc acgctcgcgc cgcagcgtgt
     5341 cgaagggcgc atcagcttcc gcaacgtcga tgtcgaatac cgttcagacg gcatcaaagc
     5401 cctcgacaac ttcaacctcg acatcagaca aggcgaacgc gtcgccctgg tcggacgttc
     5461 cggcagtggc aaatccaccg tcgtcaacct tctgccccgc tttgtcgaac cgtctgccgg
     5521 caacatctgc atagacggta tcgacatcgc cgacatcaaa ctcgactgcc tgcgcgccca
     5581 attcgccctc gtctcccaag acgtattcct gtttgacgac accctgtttg aaaacgtccg
     5641 atacagccgt cccgacgctg gcgaatccga agtcctgtcc gccctccaag ccgccaacct
     5701 gcaaagcctg atcgacgcat ccccgctcgg actgcaccag cccatcggat cgaacggcag
     5761 caacttatcc ggcggacagc ggcaacgcgt cgccattgcc cgcgccattt tgaaagacgc
     5821 gccgatatta ttattggacg aagccaccag cgcattggac aacgaatccg aacgcctcgt
     5881 ccaacaagcg ctcgaacgcc tgatggaaaa ccgcaccggc atcatcgtcg cccaccgcct
     5941 gaccaccgtc gaatccgccg accgcatcat cgtcatggat ggcggcaaaa tcatcgaaca
     6001 aggcacacac gatcaactca tgttccaaaa cggctactac accatgctga ggaacatctc
     6061 cggaaaagat accgccgccg ttcagacggc ataaacaaaa tgccgtccga aatggtataa
     6121 tcgccccgac cccttcagac ggcactatat ccgccgaccc atccgattat cttcaaccac
     6181 cgtaaaaccc attatgaccc aagacaaaat cctcatcctc gacttcggtt cgcaagttac
     6241 ccggctgatc gcccgccgcg tgcgcgaagc acacgtttac tgcgagctgc attccttcga
     6301 tatgcctttg gacgaaatca aagccttcaa ccccaaaggc atcatccttt ccggcggccc
     6361 taattctgtt tacgaatccg actatcaagc cgataccggt atttttgatt tgggcattcc
     6421 ggttttgggc atctgctacg gcatgcagtt tatggcgcac cacttgggtg gcgaagtgca
     6481 gcccggcaac cagcgcgaat tcggttacgc gcaagtcaaa accatcgaca gcggactgac
     6541 acgcggcatt caagacgacg cgcccaacac actcgacgta tggatgagcc acggcgacaa
     6601 agtgtccaaa ctgcccgacg gtttcgccgt catcggcgac actccgtcct gcccgattgc
     6661 catgatggaa aacaccgaaa aacaattcta tggcatccag ttccaccccg aagttaccca
     6721 caccaaacaa ggccgcgccc tgttgaaccg ctttgtcttg gatatttgcg gcgcgcaacc
     6781 gggctggacg atgcccaact acatcgaaga agccgttgcc aaaatccgcg aacaagtcgg
     6841 cagcgacgaa gtgattttag gtctgtccgg cggcgtggac tcttccgtag ccgccgcgct
     6901 gattcaccgc gccatcggcg accaactgac ctgcgtgttc gtcgatcacg gtttgttgcg
     6961 cctgaacgaa ggcaaaatgg tgatggatat gttcgcccgc aacttgggtg tgaaagtgat
     7021 acacgtcgat gccgaagggc agtttatggc gaaactcgcc ggcgtgaccg accctgagaa
     7081 aaaacgcaaa atcatcggcg cggaatttat cgaagtattt gatgccgaag agaaaaaact
     7141 caccaacgcc aaatggctgg cgcaaggcac gatttaccct gacgtaatcg aatccgccgg
     7201 tgcgaaaacc aaaaaagccc acgccatcaa atcccaccac aacgtcggcg gcctgcctga
     7261 aaatatgaag ctcaaactgc ttgagccctt gcgcgacttg ttcaaagacg aagtgcgcga
     7321 gttgggcgtg gctttgggcc tgccgcgcga aatggtgtac cgccacccct tcccgggccc
     7381 cggtttgggt gtgcgcatct tgggcgaagt gaaaaaagaa tacgccgact tgctgcgtca
     7441 ggcggacgat attttcatcc aagaattacg caatactacc gacgaaaacg gcacgtcttg
     7501 gtatgacctg accagccagg catttgccgt attcctgccc gtcaaatccg tcggcgtgat
     7561 gggcgacggc cgcacttacg actacgtcgt cgcactgcgc gcagtcatca ccagcgactt
     7621 tatgactgca cactgggcag agctgccata ctcactgctc ggccgcgtgt ccaaccgcat
     7681 catcaacgaa gtcaaaggca tcaaccgcgt ggtgtacgat gtcagcggca aaccgcccgc
     7741 caccatcgag tgggaataaa cagcaaacag ggctgcaccg tccggcgcag tccttcgatt
     7801 atcggaaaaa aggaaaaaat atgagcacac aagatttaag cggcaaaatc gctttggtaa
     7861 ccggcgcatc gcgcggcatc ggcgcggcaa ttgccgacac actggcggca gccggtgcca
     7921 aaatcatcgg tacggcaacc ggcgagagcg gtgcggcggc gattagcaaa cgtctggcac
     7981 aatggggcgg cgaaggccgc gtattaaatt ccgccgaacc cgaaaccgtc gaaaacctga
     8041 ttgccgacat cgaaaaaacg ttcggcaaac tcgacattct ggtcaacaac gccggcatca
     8101 cccgcgacaa cctgttgatg cgtatgaaag aagaagagtg ggacgacatc atgcaggtca
     8161 acctcaaatc cgtgttccgc gcttctaaag ccgtcttgcg cggcatgatg aagcagcgcg
     8221 ccggccgcat catcaacatc acctccgtcg tcggcgtgat gggcaatgcc gggcaaacca
     8281 attatgccgc cgcaaaagcg ggcttgatcg gttttgccaa atctatggcg cgcgaagtcg
     8341 gcagccgggg cattaccgtc aactgcgtcg ccccgggctt tatcgatacc gatatgaccc
     8401 gcgccctgcc cgaagaaacc cgccaaacat ttaccgccca aaccgccttg ggcagattcg
     8461 gcgacgcgca agacatcgcc gatgcggttc tgttcctcgc ttccgatcag gcgaaataca
     8521 tcaccggtca aacgctgcac gtcaacggcg gtatgctgat gccctaacgg acaggctttt
     8581 caaccatgcc gtctgaagcc ctttcagacg gcatttgcat tcccaaacaa aatgaacact
     8641 caccaccacc tgccctgccc ctgcggttca ggcacaagct atgccggctg ctgccgaccg
     8701 cttcatctgc gccaaatcct gccgcccacc gccgaagccc tcatgcgttc gcggtacggc
     8761 gcgtatgtgc tgcaccttat cgactacatc atcgctacta ccgttccggc gcaacaaaca
     8821 ttcctcgatg ccgccgaact gatgcagtgg agcagagaag ccgaatggct gggcttgaat
     8881 gtcatcgcac accgcaacct cggcaaacag cacgcccaag tcgaatttga agcctacttc
     8941 caagacggcg gaaaccgaac agtgcatcac gagctgtcgg cattcgtgaa aatagcggag
     9001 caatggtatt tcatcgatcc gactgttccg ctgcctacca tgaaacagcc ctgcatttgc
     9061 ggttcgggca aaaaattcaa agcctgctgc ggcaaatatc tgcaacctgt cgcataaaaa
     9121 tgccgtctga acgttcagac ggcattttca acgcgcaaaa aaaaaaccat tcataccaag
     9181 ggtaagtatg aatggtcaat acattgcggg aaaacatctt acttgctgca ctgccgaaaa
     9241 gggagaaacg gcagtgttaa ccagcaaaag cattgtacct ttattaatgt taaaaaacat
     9301 taattttgaa aatatattaa cgaatatatc tggaacctac tggttttccc gtatccaccc
     9361 gacccagcgt tcaaacagct tcggttcgag cgcggcgacg accgagcgtt tgaacacgtg
     9421 ttcaccactc caaaacccgt cgccttccaa agtcgtcagc ctgccgcccg cctcctcgaa
     9481 aatcaacgca cccgcagcat aatcccacag cttctgcccg ccgtggacat aaacatcata
     9541 acgcccgcac gccagataac accagtccag cgtgctgctg cccatactgc gtatcgtccc
     9601 gaaaggcgcg agcgtactca tgcggctgga aagtttgccc gaacgcagat atttgatttc
     9661 cacgcccgca atcgcctcat tgagtttttt atccacgaga cgcaaaggca ggcgtgtccc
     9721 attaagaaac gcaccctgcc cgcgttcggc ataaaaacat tcgccgctga ccggattgta
     9781 gattacgccc aattcggcgc gcccgttgcg gacaaacgcc accgacacgg caaaatgcgg
     9841 cagcccgttg acaaaattgt tcgtcccgtc tatcggatcg acaatccaca gccccttttc
     9901 ccccgaatat tgttcccaca aagccgactg ttcctgccgc gacatttcct cgcccaacat
     9961 cgggcaatcg atcagaagcg gcaacgcggc ggcaaatgcc gtctgcgcgg caatgtccgc
    10021 ctcgctcaac atcgaaccgt cttccttgcg gcgggacggc gtattcaaaa aacgcggcat
    10081 aatttcggtt tgcgcgatat ggcgcacgac tttctgcaaa cggtgtaaca cttcctactg
    10141 tcctcatatt ttgaacttgc ggcgcgcgaa cgtataatgt ccgtttccat cacgccgctg
    10201 cgacggatta taaccgtccg aaccgccaaa aactatgccc agattctacc tgcccgaaaa
    10261 cctttccgtc ggacaaaccg tcgaccttcc cgacaacatc gtccgccacc tcaacgtcct
    10321 gcgcatccgc cccaacgaaa acatcaccct cttcgacggc aaaggcaagg cacataccgc
    10381 acggctgacc gttttggaaa aacaccgcgc cgaagccgaa atcctgcacg aagacacaac
    10441 cgacaacgag tccccgctca acatcacgct catccaatcc atttcctccg gcgatcgcat
    10501 ggatttcacc ctgcaaaaaa gcgtcgaact cggcgtaacc gccatacagc ccgtcatcag
    10561 cgaacgctgc atcgtccgcc tcgacggaga acgcgccgcc aaacgcctcg cacgctggca
    10621 ggaaatcgtc atctccgcgt gcgaacaaag cggcaggaac accgttcccc ccgtactgcc
    10681 catcatcggc taccgtgaag cactcgacaa aatgccgtct gaaagcacca ggctgattat
    10741 gagcatcaac cgcgcctgca aactcggcga catccgccac ccgtccggcg caatcgtctt
    10801 tatggtcggg cccgaaggcg gctggacaga acaggaagaa caacaggcat ttgaagccgg
    10861 ctttcaggcg gttacactcg gaaaacggat tttacgcaca gaaaccgccc cactcgccgc
    10921 catcgccgcg atgcagacgc tttggggcga ttttacctag cagatgaaaa aacacgggat
    10981 tttatccgaa aaagcaacaa tccc
//
LOCUS       DAXDSD010000075        10979 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXDSD010000075 DAXDSD010000000
VERSION     DAXDSD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10979
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.075"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(25..1053)
                     /gene="trpD"
                     /locus_tag="ACFB2Q_001574"
     CDS             complement(25..1053)
                     /gene="trpD"
                     /locus_tag="ACFB2Q_001574"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC0918566.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
     gene            complement(1117..1707)
                     /locus_tag="ACFB2Q_001575"
     CDS             complement(1117..1707)
                     /locus_tag="ACFB2Q_001575"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC0918567.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
     gene            2118..4394
                     /gene="znuD"
                     /locus_tag="ACFB2Q_001576"
                     /gene_synonym="tdfJ"
     CDS             2118..4394
                     /gene="znuD"
                     /locus_tag="ACFB2Q_001576"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC0918568.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDPYGKRPFIAQDDQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(4934..5713)
                     /locus_tag="ACFB2Q_001577"
     CDS             complement(4934..5713)
                     /locus_tag="ACFB2Q_001577"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC0918569.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDVYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            5867..8716
                     /gene="uvrA"
                     /locus_tag="ACFB2Q_001578"
     CDS             5867..8716
                     /gene="uvrA"
                     /locus_tag="ACFB2Q_001578"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC0918570.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILGPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEEVAKVKGSYTGKYLKVVLK"
     gene            8734..9375
                     /locus_tag="ACFB2Q_001579"
     CDS             8734..9375
                     /locus_tag="ACFB2Q_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HFC0918571.1"
                     /translation="MNNNFDRSKHIYHNEAFVELVKDAIRFFHGTPVHTLPPPKQFKG
                     AGVYAIYYIGNNPLYKQYADWNRLSYNAPIYVGKAVPKGWRQARNSDNALNQSTELFH
                     RLKEHSRSIAAVSDLDPSDFMCRFVIFEGAGSDMIGTIEAALIKLHKPLWNSCVDGFG
                     NHDPGKGRYEQAKSDWDVLHSGRVWADRLNGIPNSYESILENINTHLEIIKRK"
     gene            9379..10512
                     /locus_tag="ACFB2Q_001580"
     CDS             9379..10512
                     /locus_tag="ACFB2Q_001580"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC0918572.1"
                     /translation="MKSLEIFSGAGGLAKGLELAGFQHASFIELNKDACNSLRSNFNP
                     KLVYQGDVADFDLSSQEGIEVIAGGPPCQPFSLGGKHLAHEDRRDMFPHAVRYVEYYR
                     PKAFIFENVKGLLRKSFADYFEYILLRLTYPNLGILQNEDWKGHLTRLKEIEFNLYKG
                     IKYKVSYQLLNAADYGVPQKRERVVIVGIRADLDIDWKFPKRTHSEDRLNWEKYVTGE
                     YWEKHNEPKRFNKDIAEKLQKKYGIFEPEKKPWQTVRDTLSDIPHPLGNHKITGHEYR
                     DGARIYPGHTGSGIDEPSKTIKAGGHGVPGGENMIRYDDGTVRYFTSYEAKLLQTFPE
                     EFVISGAWGEAMRQIGNAVPVKLSEILGKHLMGVLSEKSSLHN"
BASE COUNT         2898 a         3088 c         2552 g         2441 t
ORIGIN      
        1 ttgctttata tcaagtttgc cggcttaggc gaattgccgt gtaaaaccga caaactcctc
       61 ttttttcgcc ttcgccctac ctgaatcgat gccttcccgt gcggcagata tgccgtctga
      121 aagcgaagca gcgatatttc cggcatataa ggcggcggcg gtattaagca atacgatatc
      181 gcgcgccgcc ccttctttcc cgtccagtac ctcgttcatt ttcaacaaag attcctgagt
      241 attggcaact ttgatttcat ccaaattgcg gcgggtttcg ataccgaaat cttctgggcg
      301 gatgtcgtat tcgctgattt ttccgtcttt gagttcggca acgcgcgttt tgcccgtcag
      361 tgtaatttca tccaaaccgc cctccccgca aacgaccaaa acgtgttttg agccgagttg
      421 ttgcaagacc cgcgacaaaa tgccgcacaa atcggtgtgg aacacgccca aaagctggtt
      481 cggcgcgccc gcaggattgg ttaacggacc caatatgttg aaaatgcttc ggaaaccgag
      541 cgaacggcgc accggggcga catggcgcat ggcactgtgg tgattgggtg cgaacataaa
      601 ccctataccg gtctgcctga tactttgggc aatctgttcg ggagtcaggt tgaggtttgc
      661 gcccatttgc tccatcacgt cagccgcacc gctggaggaa gagaccgacc ggcctccgtg
      721 tttggcaacc tttgcgcctg ccgctgcagc aacaaacatc gaagtcgtcg aaatattgaa
      781 ggttttcgcg ccatccccgc ccgtaccgac gatatcgacc agcccgtccg catcctccag
      841 cggcactttt gacgcaaact cgcacatcac ggcggcggcg gcggtgattt cggaaacggt
      901 ttcaaccttg atacgcaagc cggtcaaaat cgccgcaatt tgttcgggcg gcacttttcc
      961 gctcatcatt tggcgcatca agtcggtcat ttcatcgtaa aacaactcgt tattgctgat
     1021 taatcgttcg atagcctgtt gcggtgtaat cattttttgt cctccgttca atattcggac
     1081 gaaaatgccg cccgaagggc ttcagacggc atcacgtcag attttttgcg gtttgaagtt
     1141 ttgaaattca actaaaaaat tgttcaacat atcatgtccg cgttcggtca agagggcttc
     1201 ggggtggaac tgcacgccct cgacggcata ttccttatgg cgcacgccca taatttcgcc
     1261 gtcttccgtc cacgccgtga tttccaagca atccggcagc gtgccgcgtt cgataacgag
     1321 gctgtgataa cgcgtacagg taaccggatt gggcaaaccc ttaaacatac ccttgcccga
     1381 atgggacacg ggcgacacct taccgtgcat caaggttttt gcccgtacca catctccacc
     1441 gaacgcttcg cctatcgtct gatgcccgag gcacacgccc ataatcggca gccggccggc
     1501 aaaatggcgc atggcttcta ctgaaatacc cgcctcctta ggggaacacg gtccgggacc
     1561 gataacgaga tattgcggat tcaatgcctc gatttcctcc aacgtaatat catcgttgcg
     1621 gcgcacggcg acttcctgcc ccaattctgc gaaatactgg acgatgttgt aagtaaaact
     1681 gtcgtaattg tcgataaaca aaagcatttt gtgattaacc tattgattta taatttgttt
     1741 attattaact gcatttccga tacccccatc cataccccct tatatcttag cgtgcccgat
     1801 gcgccctcgt gaatctggcg cagagcctcg cgttctgaca agtacgccat tttgccacct
     1861 tttcgaccgt tcgggaacgt accgttttta tgccctcata aattttggtt atccgatagc
     1921 taacatatag cggcgttcgt gttcacatca ttaacttccc taaaattagg gttaatacaa
     1981 attaacgcca ttaaatatca caaccgtgtc atttgaaaaa cttttgccta tgccctctga
     2041 gtattctcaa aatcttatga tataaaaaat gcaatgttat ataataacaa acttttgaaa
     2101 tgcgacgaga agccaaaatg gcacaaatca cactcaaacc cattgtttta tcaattcttt
     2161 taatcaacac acccctcctc gcccaagcgc atgaaactga gcaatcggtg ggcttggaaa
     2221 cggtcagcgt cgtcggcaaa agccgtccgc gcgcgacttc ggggctgctg cacacttcga
     2281 ccgcctccga caaaatcatc tccggcgata ctttgcgcca aaaagccgtc aacttgggcg
     2341 acgctttgga cggcgtaccg ggcattcatg cctcgcaata cggcggcggc gcatccgctc
     2401 ccgttattcg cggtcaaacg ggcagacgga ttaaagtatt gaaccatcac ggcgaaacgg
     2461 gcgatatggc ggacttttct cccgatcacg ccattatggt agataccgcc ttgtcgcaac
     2521 aggttgaaat cctgcgcggg ccggttacgc tcttgtacag ctcgggcaat gtggcggggc
     2581 tggtcgatgt tgccgatgga aaaatccccg aaaaaatgcc tgaaaacggc gtatcgggcg
     2641 aagccggatt gcgtttgagc agcggcaatt tagaaaaact gacatccgca ggcatcaata
     2701 tcggactggg caaaaacttc gtgctgcata ccgaaggctt gtaccgcaaa tcgggcgatt
     2761 acgccgtacc gcgttaccgc aatctgaaac gcctgcccga cagccatgcc gattcgcaaa
     2821 cgggcagcat cgggctgtct tgggtaggcg aaaaaggctt tatcggcgca gcatacagcg
     2881 accgtcgcga ccgctacggc ctgcctgccc acagccacga atacgatgat tgccacgccg
     2941 acatcatctg gcaaaagagt ttgatcaaca aacgctattt gcagctttat ccgcacttgt
     3001 tgaccgaaga agacatcgat tacgacaatc cgggcttgag ctgcggcttc cacgacggcg
     3061 acggtgcaca cgcacacacc cacaacggca aaccgtggat agacctgcgc aacaaacgct
     3121 acgaactccg cgccgaatgg aagcagccat tccccggttt tgaagccctg cgcgtacatc
     3181 tgaaccgcaa tgactaccac cacgacgaaa aagcaggcga tgcagtagaa aacttcttca
     3241 acaacaaaac acacaacgcc cgtatcgagt tgcgccacca acccataggc cgtctgaaag
     3301 gcagctgggg cgtgcaatat ttgggacaaa aatccagcgc gctttccgcc attcccgaaa
     3361 ccgtccaaca accgatgttg attgacaaca atgtccgcca ttacagcttt ttcggtgtag
     3421 aacaggcaaa ttgggacaac ttcacgcttg aaggcggcgt acgcgtggaa aaacaaaaag
     3481 cctccatccg gtacgacaaa gcattgattg atcgagaaaa ctactacaac cagcccctgc
     3541 ccgacctcgg cgcgcaccgc caaaccgccc gctcgttcgc actttcgggc aactggtatt
     3601 tcacgccaca ccacaaactc agcctgaccg cctcccatca ggaacgcctg ccgtcaacgc
     3661 aagaactgta cgcacacggc aagcacgtcg ccaccaacac ctttgaagtc ggcaacaaac
     3721 acctcaacaa agagcgttcc aacaatatcg aactcgcgct gggctacgaa ggcgaccgct
     3781 ggcaatacaa tctggcagcc taccgcaacc gattcggcaa ctacatttac gcccaaacct
     3841 taaacgacgg acgcggcccc aaatccatcg aagacgacag cgaaatgaag ctcgtgcgct
     3901 acaaccaatc cggtgccgac ttctacggcg cggaaggcga aatctacttc aaaccgacac
     3961 cgcgctaccg catcggcgtt tccggcgact atgtacgagg ccgtctgaaa aacctgccgt
     4021 ccctacccgg cagggaagat ccctacggca aacgtccctt catcgcgcag gacgaccaaa
     4081 acgccccccg cattccggct gcgcgcctcg gcttccacct gaaaacctcg ctaaccgacc
     4141 gtatcgatgc caatttggac tactaccgcg tgttcgccca aaacaaactc gcccgctacg
     4201 aaacgcgtac gcccggacac catatgctca acctcggtgc aaactaccgc cgcaatacgc
     4261 gctatggcga gtggaattgg tacgtcaaag ccgacaacct gctcaaccaa tccgtttacg
     4321 cccacagcag cttcctctct gatacgccgc aaatgggccg cagctttacc ggcggcgtaa
     4381 acgtgaagtt ttaaaatcgg acaggcaaac aaaaaaggtt ttcagacggc attctgaaaa
     4441 cctttttttc tttttaatca acttgttagg aaatatggca aagacaagga acaaaccact
     4501 gtgccatcat tcccattttc tttgtttcat cattcttgct ttcacgccgt cattctcgca
     4561 aaagcagaaa aatcaaaagc aaaaacccaa gatcccatca ttcccgcgca ggcgggaatc
     4621 caattcattc ggtttcagcc gtttccgata aatcaccgta acgttaagtt cccggattcc
     4681 cactttcgtg ggaatgacgg cgtaaaggtt tctgtttttt aagttttagg taactaatga
     4741 gtcgtcattc ccgcgcaggc gggaatccaa ttcgttcggt ttctgttatt tctgataaat
     4801 caccgtaacg ttaagttccc ggattcccat tttcgtgaac ataacggtgt gaacattagc
     4861 aaaaattcaa aaaaccaaac ttcctcattc gtgtgaatga tggtactgat attttctatt
     4921 tcagttcgca aaattaatct ttcaacacat cttgcacagc cgcctgaatc ttagccgccg
     4981 ccgcttcgat ttgctgctgt tcggcagatg ggtttgccgc tgtttcaacc gaagcagctt
     5041 gtgaaacagg atcggcttga gaatcagtca gcggcaaacg cgccaatacg gtttttacgc
     5101 cggttacttt atcaccaatc gccacttgcg cctgcgcatc gacaggcaga tacacatcca
     5161 cgcgcgaacc gaagcggata aagccatagc gttcgccgcg ggacagtttc gcacctgctt
     5221 gggtgtaaca caaaatacgg cgtgccacca gaccggccac ttgaacaaaa gtaatttcac
     5281 gaccggaagc cgtagtcgcc aaaaccgcgt tgcgttcgtt ttccgtgctg gctttgtcca
     5341 aatccgcatt cacgaatttg cctttgttat agaccacttt cgttaccgta caatcggcag
     5401 gcgatttttg cgaatgcacg ttgaacacgt tcataaaaat actgattttc aaagcatcga
     5461 catcacgata cggatcgcgt gcgcgctcga ccaccacgat acggccgtca accggactca
     5521 acaccgcttc aggattttgc ggaatttcac gcgcagggtc gcggaaaaac tgcaatgcaa
     5581 atacggtaaa cacccaaaac ggcaaagacc accagccgca gcacatcgac accagcaggc
     5641 tcaaagccaa accgccgccg ataatcggcc agccctcacg ggcgataatc gggtggggat
     5701 aaagacggtt catttgtcat ccttttattc aaatatatta aatagcgaca ttataacgga
     5761 atccgccgcg ttccgccgac aaatgccgtc tgaacgcggc ttcagacggc atccgagaaa
     5821 gtgctacaat atcggttttc ctttattttt tcaggcggca tccattatgt gcaaccatca
     5881 tccccgacat tcgcacgaca acgacaccat ccgcattcgc ggcgcgcgca cgcataattt
     5941 gaaaaacatc gatttggaca ttccccgcca caagctcgtg gtggtaacag gattgtcagg
     6001 cagcggcaaa tcatcgctgg cgtttgatac tttatatgcc gaagggcagc ggcgttatgt
     6061 cgagagcctg tctgcctatg cgcggcagtt tttgcagatg atggacaaac ccgatgtcga
     6121 tttgattgaa ggcctgtcgc ctgcgatttc catcgagcag aaatccacca gccacaatcc
     6181 gcgttccacc gtcggcacgg taacggaaat ccacgactac ctgcgccttt tatacgcccg
     6241 tgtcggcacg ccgtattgcc ccgaacacaa gctgccgcta tccagccaaa ccgtatctca
     6301 aatggtcgat gccgtattaa aactgccgga agatacgcgc gtgatgattc tggggccggc
     6361 ggtgcgcgag cgtaagggcg agtttgtcga tttctttgcc gacttgcagg cgcaaggctt
     6421 tgcccgtgtg cgcgtggacg gcgaagtcta tcagcttgac gaagtaccga agctggaaaa
     6481 aaacatcaaa cacaatatcg acgtcgtcat cgaccgcgtg aaagtgaaag ccgacatcaa
     6541 acaacgactg gcggaaagtt ttgaaaccgc gctgcgccac ggcaacgagc gcgcgctggc
     6601 gatggaaatg gacagcggcg aagaacattg gttttccgcg cgttttgcct gccccgtatg
     6661 ctcatacagc ctgcccgaat tggaaccgcg cctcttttcg ttcaacaacc cgatgggttc
     6721 ctgcccgact tgcgacggct tgggcaacac caatttcttc gaccccgaaa aagtagtcgc
     6781 ccatcccgaa ttgtcgctgg caaccggcgc gattgacggt tgggacaaac gcaaccaatt
     6841 ctatttccaa atgattcaat cgctggcgca tcattataaa tttgatgtca acgtcgcttg
     6901 ggaaactttg cctgaaaaag tcaaaaaagt cgtgttgcac ggctcgggca aagaagtcat
     6961 tgatttcacc tacctgtccg aacgcggcac caccttcaac cgcagccacg ccttcgaggg
     7021 catcatcccc aatctcgaac gccgctaccg cgaaaccgac agcgaaaccg tgcgcgaaaa
     7081 actgcgcgag taccaaaatc accgtgcctg cccgagctgc ggcggcgcac gtttgcgcaa
     7141 agaagcacgc tacgtttacg tcggcggcga accgttgcac gaagtctccg cctggccgct
     7201 gaccaaaacc caccgattct tcgaaacgct ggatttggac ggcaacaaaa aacagatcgc
     7261 cgaaaaaatc ctcaaagaaa tcaccgagcg gctcggcttc ttgattaacg tcgggctgga
     7321 ttacctgaat ttaagccgtt ccgccgaaac cctttccggc ggcgaagccc agcgcatccg
     7381 cctcgccagc caaatcggca gcggcctgac cggcgtgatg tacgttttgg acgaaccctc
     7441 catcggcctg caccagcgcg acaacgaccg cctgctcgcc accctcaaac gcctgcgcga
     7501 tttgggcaac agcgtaattg tggtcgaaca cgacgaagac gccatccgcg aagccgattt
     7561 cgtcgtcgat atgggccccg gcgcgggcga acacggcggc aacgtactga ttgccgacac
     7621 acccgaaaat gtcgccaaat gcgaaaaatc cgttaccgga caatacctcg gcggcaaaaa
     7681 atccattgcc gtgccgtctg aacgcacgcc cgtcaatccc gaccgaatgc tcgtcctcaa
     7741 aggtgcgcgc ggcaacaacc tcaaaaacgt caccctcgag ttgccgctcg gtttgattac
     7801 ctgcatcacc ggcgtatccg gcagcggcaa atccaccctg attaacgaca ccctcgccaa
     7861 aatcaccgcc cgcgaactca accgcgccca agaagaaccc gccccatacg acgacatccg
     7921 cggcctcgaa cacctcgaca aagtcatcaa cgtcgaccaa tcccccatcg gccgcacccc
     7981 gcgctccaac cccgccacct acaccggcct gtttaccccc atccgcgaac tcttcgccgg
     8041 cgtacccctc tcgcgcgaac gcggctacaa cgtcggcaga ttctccttca acgtcaaagg
     8101 cggccgctgc gaagcctgcc aaggcgacgg cgtaatcaaa gtcgaaatgc acttcctgcc
     8161 cgacgtgtac gtcccctgcg aagtctgcca cggcaaacgc tacaaccgcg aaaccctcga
     8221 aatccaatac aaaggcaaaa acatcagcca agtcctcgac atgacggtcg aagaagcccg
     8281 cgaatttttc gacgccgtcc ccaccgtatc gcgcaaactg caaaccctga tggacgtagg
     8341 tctcggctac atccgcctcg gccaatccgc caccaccctc tccggcggcg aagcccagcg
     8401 cgtcaaactc gccttggaac tctccaaacg cgacaccggc agaacgctct acatcctcga
     8461 cgaacccacc accggcctgc acttcgccga catcgccctg ctgctggaag tcataggccg
     8521 tctgaaaggc aaaggcaact cgattgtgat tatcgagcat aatctggatg taattaaaac
     8581 cgcggattgg attgtggact tagggccgga aggcggcgat ggtgggggta aggttatcgc
     8641 gaaaggtagc cctgaggaag tggcgaaggt taaggggagt tataccggga agtatttgaa
     8701 agtggttttg aagtaaacga acggatatag aaaatgaaca ataattttga ccgcagcaag
     8761 catatctatc ataacgaagc ttttgtagaa ctggttaaag atgccatacg cttttttcat
     8821 ggtacgcctg ttcatacact tcctccaccc aaacagttca aaggtgcagg ggtttatgcg
     8881 atttactata tcggtaacaa tcctttgtac aaacaatatg cggactggaa cagattatcc
     8941 tacaatgcgc caatttatgt tggcaaggcc gtccccaaag gctggcggca agcaagaaat
     9001 tctgataatg cgctaaacca atcaaccgaa ctgttccacc ggttgaaaga acacagcagg
     9061 agtattgccg ctgtttctga tttagatcct tccgatttca tgtgcagatt cgttatcttt
     9121 gaaggtgccg gttctgacat gataggcaca atcgaagctg ccctgattaa attacacaaa
     9181 cctctatgga actcttgtgt cgatggtttc ggaaaccacg accccggaaa aggccgttac
     9241 gaacaagcca aatcggattg ggatgtcctt catagcgggc gggtttgggc agataggcta
     9301 aacggcatac cgaatagcta tgagtccata cttgaaaata tcaacacaca tttagagata
     9361 ataaagagaa aataacctat gaagtctttg gaaatttttt ccggcgcggg cgggttggca
     9421 aaaggccttg aacttgcagg attccaacat gcatctttta ttgaactcaa taaagatgca
     9481 tgcaattctt taagaagcaa ctttaacccc aagcttgttt atcagggaga tgtggcagat
     9541 tttgatcttt ccagtcaaga aggcatagaa gtcattgcag gcggtcctcc ttgccagccc
     9601 ttttcattgg gcgggaaaca ccttgcccat gaagacagaa gggatatgtt tcctcatgcg
     9661 gtcagatatg tggagtatta ccgccccaaa gcttttatct ttgagaatgt aaaaggactt
     9721 ttaagaaaat cattcgctga ttattttgaa tatatcctac ttcggcttac ttatccgaat
     9781 cttggtattt tgcagaatga agattggaaa ggccatctga cacggctgaa agaaattgaa
     9841 ttcaatttat ataaaggaat caaatataag gtttcatatc aattgttgaa tgcagctgat
     9901 tacggagtgc cgcaaaaacg cgaacgcgtg gttatcgtag gtatccgggc cgatttggat
     9961 attgattgga aattcccaaa aagaacacat tccgaagaca ggttgaattg ggaaaagtat
    10021 gtaacggggg aatactggga aaaacacaac gaacccaaaa gattcaataa agatattgct
    10081 gaaaagttac aaaaaaaata cggtatattc gaaccagaaa aaaaaccttg gcaaacggta
    10141 agggatacct tgtccgacat cccgcatcct ttggggaatc ataaaattac aggacatgaa
    10201 tatagggatg gcgcaagaat ttatcccgga cacacaggaa gcgggataga cgaaccgtcc
    10261 aaaaccatta aagcaggtgg gcatggcgtt cccggcggag aaaatatgat tcgttatgat
    10321 gatggaacag tcagatactt taccagctat gaagcaaaac ttcttcaaac attccctgaa
    10381 gaatttgtca tttctggagc ttggggagaa gcaatgcgac aaattggcaa tgcggttcct
    10441 gtcaaattgt cggaaatttt aggcaaacat ctgatggggg tgttgtcgga gaaaagcagc
    10501 ctgcacaact aacccgccca ctgcgtgtgt gcggtacgca cgcggttgct tgattgacgg
    10561 tttgccgttt caacttttaa cttcgttgaa gctagtgctt tcagacggcc tccaatgtcg
    10621 tttggaaaca tcaggtccaa caaagttaaa ccttaaacaa caaaccattc cttctccaac
    10681 acccaaacca caaacaaaaa tcctatggcg cgttatcgca gaaacttcat tgccggcggc
    10741 acattctttt tcaccgtcaa acccgccgcc ccgaaatcgc gtctgcttgt cggacatatc
    10801 ggcttcgcgt gcggcttata tggatgtgca aaaacaatat ccctttgaaa ccgtcgccgt
    10861 atgcgtgctg ccgaaccata ttcacgccat ttggacgctg ccgcccgacg atgcggatta
    10921 ttccctactc cggcggctga ttaaaaccaa attctccgca tattcccctc atactaaaa
//
LOCUS       DAXDSD010000076        10775 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXDSD010000076 DAXDSD010000000
VERSION     DAXDSD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.076"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..453)
                     /locus_tag="ACFB2Q_001581"
     CDS             complement(<1..453)
                     /locus_tag="ACFB2Q_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC0918573.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQA"
     gene            complement(572..1816)
                     /gene="ftsA"
                     /locus_tag="ACFB2Q_001582"
     CDS             complement(572..1816)
                     /gene="ftsA"
                     /locus_tag="ACFB2Q_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC0918574.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            complement(1902..2630)
                     /locus_tag="ACFB2Q_001583"
     CDS             complement(1902..2630)
                     /locus_tag="ACFB2Q_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC0918575.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            complement(2620..3534)
                     /locus_tag="ACFB2Q_001584"
     CDS             complement(2620..3534)
                     /locus_tag="ACFB2Q_001584"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC0918576.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            complement(3646..5055)
                     /gene="murC"
                     /locus_tag="ACFB2Q_001585"
     CDS             complement(3646..5055)
                     /gene="murC"
                     /locus_tag="ACFB2Q_001585"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC0918577.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            complement(5210..6277)
                     /gene="murG"
                     /locus_tag="ACFB2Q_001586"
     CDS             complement(5210..6277)
                     /gene="murG"
                     /locus_tag="ACFB2Q_001586"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC0918578.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(6281..7552)
                     /locus_tag="ACFB2Q_001587"
     CDS             complement(6281..7552)
                     /locus_tag="ACFB2Q_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC0918579.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(7731..9068)
                     /gene="murD"
                     /locus_tag="ACFB2Q_001588"
     CDS             complement(7731..9068)
                     /gene="murD"
                     /locus_tag="ACFB2Q_001588"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC0918580.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(9153..9533)
                     /locus_tag="ACFB2Q_001589"
     CDS             complement(9153..9533)
                     /locus_tag="ACFB2Q_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC0918581.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQQ
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            complement(9658..10740)
                     /gene="mraY"
                     /locus_tag="ACFB2Q_001590"
     CDS             complement(9658..10740)
                     /gene="mraY"
                     /locus_tag="ACFB2Q_001590"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC0918582.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         2476 a         3094 c         2740 g         2465 t
ORIGIN      
        1 tgcctgtgcc acatggacgc gtttgccttc ataagaaaac ggacgggtaa ctacggcaac
       61 ggtcagaatg cccaaagact tggcaatctc agcaacaacc ggcgcggaac cggtaccggt
      121 accgccgccc ataccggtcg tgataaacag catattggca ccgcgaatgg cttcttcaat
      181 ggcctcccga tcttcctgtg ctgccgcacg gccgatatcg ggattcgcac ccgcgcccaa
      241 accgcgtgtc agattcgtac ccaactggat tctcttcgcc gcatggtttt ttgccaaaga
      301 ctgcgcatct gtattggcac tgataaactc cacactgcgc acattgttgg caaccatgtt
      361 attgattgca ttgcaaccgc cgccgcccaa gccgattact ttaattaccg cagggctgac
      421 tgccgattct gccacgtcgt aaacaaattc cattcaaaaa ctcctgatcg ccccgttcag
      481 aggacggttt aaataaatta ttatttatta tataagaagt atcttactgc tggcaaaata
      541 cttctcacct gtcaaacggc aatccaactg ttcagaggtt gttttcaatc caccgcttca
      601 atcttgccaa caaaccgccg cccccttctc tctcttgcac tgtaccgttt tccggttgcg
      661 gcaagtttcc ttccagcttg catgctgcat gaagcagccc gatagcggta gaaaagcgcg
      721 gtgtgcggac acggtcggac aaaccgccca tttcttgggg cgcaccggtg cgtacaggca
      781 aatcgaagat tttttcggca aattccacaa tcccggtcat catggacaca ccgccggtca
      841 gaacgatacc cgcattcagc acttctttgg ggaaacccga tttttgcagt tcgcccagca
      901 ctacgccaaa aatctcctga atacgtgcgc tgatgattgc tgccaaaacc ttactggaaa
      961 cctgacgcga tgtccggtca cccacgcccg gaacttcaat catctcgccc aagccttccg
     1021 tatcgcatga tgccacgcca taatggattt taatgtactc ggcggcatcg agaggtgttc
     1081 tcaacgattt ggacaaatct ttggtaatca gattaccacc ggccggaatg acggacgtat
     1141 ggcggatggc gccgttcata taaacggcaa tatcggtcgt tccgccacca atgtcaatga
     1201 cgcatacgcc gaggtctttt tcatcttcag tcagcaccgc ctgcccgctt gccaacggtt
     1261 gaagcatgat ctgatcgctt ttcaagccgc acagctcgat acatttttgg acattctgca
     1321 ctgccgtact tgcaccggta atgatgtgca cccgcgtatc cagacgcaca ccgctcatac
     1381 cgatgggctc ccttacacca agttgggtgt cgataatgta gtcttgaacc acggcatcga
     1441 gaattttttg atcgggcggg atattgattg cctttgccgt ttcaatggcg cggtcgatgt
     1501 ctgcctgtgt tacttctccg tctttgattt taaccacacc ttgcgaatta aggctgcgga
     1561 tgtggttgcc tgcgatacct gtggtaacgt gggtaatttt ggtatccgcc atcagctcgg
     1621 catcattgac cgcctgcctg atggcttgga cggtggcatc gatattggtt accatgcccg
     1681 cgcgcaagcc ccgtgaagga gcctgcccca aaccgatgat gttgattttg tcgtcatctt
     1741 gaacttcccc gatcagtgcg aggactttag acgtaccgat atccagtacg ctgatgtatc
     1801 ttttctgctg ttccattgtt cgtctgccct taaaactgat tgaaatttgc gtcgcaccgt
     1861 ttcagacggc acggccgtaa tatgtccgat acctgttccc actattcttc ggattctttt
     1921 tcgggtaaac cgtcgggagc atggcggact gaaaatccgt ccttatacct catatccaca
     1981 taggataacc gatttttatt cttacgcaac agatgctgcc acgcttcggt aaaaagccgg
     2041 aggcgtttca tctcgttttc ccgtccgagc ctgacggtga tgccgttgtc caaaacgaca
     2101 ttccacgccg aacgtgccgt ataggtcatc tctttgatgc ccaaaccctg ttttgccaaa
     2161 acagtcgaaa attcgtcata acggcggagc atttcggcag acgttccttc cgcgcctctg
     2221 aataccggca ttccgggtct gtccaagcgg gcttcaaaaa cattgccttc gccgtccacc
     2281 aaggcatggt cgccccaacg tgcaaccggc ttgcgctcgg tcaggacgac ctcaaccgta
     2341 tcgggaaaac ggcggcgcac catgaccgac gcaatccacg gataccggcg gtaggcttcc
     2401 tgtgcgccat tgatgtccgt cctcaaaata ttcccatgga tgtattcttt cgccaaactg
     2461 cccaatgcct tcttatcgga ataaaccagg ttgcccttca gcgacacctg cttgacgggc
     2521 agatgattcg aattgtaaaa ccaaaccagc ccggacgcag caagcagcat cgccatcatg
     2581 acaagcagcc agcgcgtcag ccgttccatc gcttcggcat tatcccacat gtgcggcctt
     2641 caaaatttca atacataaat cggcaaaacc cacgcccgta acggcagcgg atttcggtac
     2701 taaactatgg ccggtcatac cgggcagggt gttgatttcc aacagataga gtttgccgtc
     2761 ggtatctttg aggaaatcga cgcgcacgca gccttccgca ccgattgcct gtgcgccgcg
     2821 aaccgccagt tcgcgcatca ggctttcttc ggcttcggtc aaatcttccg aaggacattg
     2881 ataaatggtg tcgtctcggt tgtacttggc ttcgtagtcg taaaactcgg ttgcggggat
     2941 gatgtgtatg ccgggcagcc ctttgccgtt caggacgggg caggaatatt cgccgccgcc
     3001 gataaaacgt tcggcaatga tttcgccctg aaggtgtttc aattcttcgt aaacgctttt
     3061 cagacggcct ttttctttga cttttaccac gccgacgctg ctgccttcgg ccgccggctt
     3121 cacaaacatc ggcagaccca atttttcttc gacggcatcg aaatcggtat catcgtacag
     3181 tacggcgaac tcgggaacgg gtaatcccaa tgcctgccaa atcagtttgc agcggtattt
     3241 gtccatgccg atggcggagg cggcgacacc gctgccggta tagggaatgc ccaacagttc
     3301 caatgcaccc tgaacagccc cgtcttcgcc gtaagtaccg tgaaggatgt tgaatgccgt
     3361 ctgaaagccc cgctccttca gttcggataa cggcgtttcc ttagggtcga aggcgtatgc
     3421 gtctatgcct ttgcttttta aggcgttcaa aatggcggta ccgctgtcca gcgagatttc
     3481 tcgttcgctg gaaaaaccac ccatcaatac ggccactttg ccaaaattct gcattgtttt
     3541 tgttctttcc taattgcttt atgcttgttg tcagaggtcg tctgaaacct gattgcggtt
     3601 tcagacgacc tttatatgat gttccgtctg tcaggcgggt gtatctcaaa tctgtttcga
     3661 caattccaac agcgcggagg gaacgcggtt gatgcttccc gcacccatat tcaacacaac
     3721 atcgccatcc tgtaaaacat tcatcagcat ttgcggcagg tcggcgacgt tttcgcagta
     3781 aatcggctca agtttgccca atacgcggat agcacgcgcc aaggcgcggg agtcggcggc
     3841 ggcaaccggc tcttcgccgg cggcataaac ttcggtcagt accagcgcat caacggtatt
     3901 gagtactttg gtaaagtctt caaacaaatc gcgcgtgcgg gtatagcggt gcggctggaa
     3961 ggcgagtacc aaacgttttt ccggatacgc gccgcgtgca gcggcaaggg ttgccgccat
     4021 ttcgacgggg tggtgtccgt aatcgtccac cagcaaagcg gtcccgccgt ttggcaactt
     4081 gatgtcgccg tatttttgga agcggcggcc gacgccttca aagccgagca agcctttttg
     4141 gatcgcttca accgatgcgc cgacttccag cgccacgccg atggctgcca atgcgttcag
     4201 cacgttgtgt ctgccgggca tattcagcac gacttcaaac gacccctgct catgtccttt
     4261 catttgaaca tggacggtga atttcatttg cgcgccgacg ttttcgatgt cggtggcgta
     4321 gatgtcggcg gtatcgtcca aaccgtaagt agcataaggt ttgctcactt tgggcaaaat
     4381 cgcgcggacg tgttcgctgt caacacacaa aaaggctttg ccgtagaagg gcatacggtg
     4441 gatgaaatcg ataaacgcct gatgcagttt ttcgacgctg tgcccgtagg tatccatatg
     4501 gtcttcgtcg atattggtaa cgacggacat aatcggggtc agatgtagga aagaggcatc
     4561 ggattcgtcg gcttcggcaa cgatgtattc gcctttgccc aagcgggcgt tggtgcctgc
     4621 ggcgttgagt ttgccgccga taacgaaagt ggggtcgagt cctgccgcgc cgaggatgga
     4681 ggcggtcagg ctggtggtcg tggttttgcc gtgcgtaccg gcaatggcga tgccgtcacg
     4741 gaagcgcatc agctctgcca gcatcaaggc gcgcggaata acgggaattt gccgctccaa
     4801 cgcagcgaca acttcgggat tttctttctt gacggcggta gaggcaacga cgacatccgc
     4861 accgttaacg tgttctgcgg tatggccggg ataaacttga atgcccaggc tgctcaaatg
     4921 ctcggtagcg gcatttcgcg cctgatccga accggaaact ttaaagccca aattgtgcaa
     4981 gacttcggcg ataccgctca tgccgacgcc gccgataccg acaaaatgga tgttgcttac
     5041 tcgatttttc atcataatgt tgcgttccgg ttggttttta gtgcgtaaag gcgttatttt
     5101 aaaggtctga ccgtttgcgc accatagttt tctgacaaat atatagcgga ttgaaataaa
     5161 aaacatccat gccgtctgaa cggcttttca gacggcatgg ttcggcagtt tacgccgcac
     5221 acgcaatcgc ggcttcggca acgtcatccg cgctgtgcgg caatgccaac gtacgggcgt
     5281 tttccgccca tttgaggcat ttttcgcggt tgaggctgcc gaggatttcg gcgagttttt
     5341 ccgccgtcaa ctgggtttgc ggcaacagca gccccgcttc tgcctgcacc atgaaacgcg
     5401 cgttggcggt ttgatggtca tcaacggcgt gaggatacgg cactaacaac gcgcccagcc
     5461 ccgccgccgt caactcggca atcgtcagcg cgccggcacg gcaaatcacc aaatcggcat
     5521 cacggtaggc ggacaccatg tcggtaataa attccacgca ttccgctttc acgcccaacg
     5581 cgtcataatc cgcctgaaga ttgcccagct tgttacgccc cgactggtgg tacatctgcg
     5641 ggcgcacctc ttcaggcagc agtgccaacg cctgcggtac ggttttgttc aaaacgtccg
     5701 cacccaaact gccgccgacc accaaaattt tcagacggcc ttcgcgccct tggaagcgtt
     5761 cggcaggcac gggcaggttg ctaatatcgg cgcggacggg gttgccgacc aaaccgcctt
     5821 cgtggctgaa cgctttcgga aaagcgtaca acacccgttt cgcccagcgc gacaggtggc
     5881 ggttggacaa gcctgccacg gcgttttgct cgtgaatcac aatcggcacg cccaagagtt
     5941 tcgccgccag accgccggga aaggtaacaa aaccgccgaa gccgatgacg cactcgacac
     6001 ggtgtttgcg gataatccgc tgcgcttcgc ggacggtttt gtacagagta aacggaagca
     6061 tcagcttgcg tttgatgccg ttgccgcgta ttcctttaat cgccagcgtt tccaagcgta
     6121 tgccgtattg cggcacgatg cgctcttcca tcgaatcctt gctgcccagc caaattacat
     6181 gatgaccgcg cacgcgcaat gaatccgcca cagccagagc tgggaaaatg tgtccgcccg
     6241 ttccgcccgc catcagcata aaggttttac cgcccatgat ttactccacc cggtaaccgc
     6301 gcattttccg gcggttttca taatcgatac gcaacagcag catcatgctg atcagcatga
     6361 aaaagactga cgaaccgcca taggacatca acggcagcgt cagacctttg gtcggcaaag
     6421 caccgatgtt cacaccgata ttgaagaaac tttggatacc gatccaaatg ccgatacccg
     6481 aagcgatata ggcgttgaaa gtcaaaccca aatcgcgcga ctgcttgccg atggaaaacg
     6541 cccgcaccac cagccagccg taacagaata tcagcacgca catcccgaag aagccgaatt
     6601 cttcagcgat gatggcaaaa ataaaatcgg tatgcgcttc cggcagaaag ccgcgtttgc
     6661 tcaaactcgc acccaaaccc ataccgaacc actctccgcg cccgattgcc atcagagagt
     6721 gggtaagctg gtagccggca ccctgcgggt ctttccacgg gtccaaaaat gccactaccc
     6781 gctgcacacg gtagggagcg gcggtaatca tcagcaccat cccacccaag acgctgccta
     6841 ccaggacaaa aaaatatttc cacggcaatc ctgccagaaa cagcattcca acggtaatga
     6901 cggtaatgac gacaaacgaa ccgaaatccg gctgtaccat tatcagcacc aaaccgaacg
     6961 ccaccagcat aatcggcagg atgatcgccc ggaaacggcc gtacatttct aatgtttcac
     7021 gacgtgcctg cggattggtg gcggacataa tcaggttggc cgtcccccgc caaatcgact
     7081 gccaacccaa actttccatg ctgcgcaaca cttcttcacg gcgcgtgaac aggcttgcca
     7141 aataaaggat gactgccagc ttgaacagct cggtcggctg gaaattcaac ggacccaaag
     7201 gtatccaacg ggtcgcgcca ttgatttcgc gcccggcaat caatacggct accagcaaca
     7261 ggccggataa ggcaaaaatc cacggcacaa gccgccgcca tgtcctcatc ctgcaaagaa
     7321 accataaaaa accgctcgct ataaggccgg caacgacgaa ccccgcctgc ctggtcaaat
     7381 agaaaaactg atcgccgcct tccttcgatg ccaaatacac agaagccgaa taaatcatca
     7441 gcaggccgaa cgccgtcatc agcaccacca tccacaaaag cggcgcgtcg aatttcctgc
     7501 cgtcgcgcac aatcggcctg tcgagcagca gagtgtggac accgtcgccc acttttacca
     7561 atacttccga aatcttcaaa aaaccacctg ccagtctgtt tgcacctgcc gcaaagggca
     7621 aaaatttcag acggcagaca atgccgtccg aacatacgat acatcccaaa tcggtattct
     7681 aaatctttac ttgccgccca acaatgacgg cgtttgtgtt tcggacggca tcacaaagcc
     7741 ttaaacgctt cgataaacac ttccgaacgg tgcgcgtagc ctttaaacat atcgaaactc
     7801 gcgcaggcgg ggctgagcaa gacaatatcg cccgcttcgg cttgggcgta tgccgtctga
     7861 accgcctctt ccaaagtgac gcagtcggtc aggttcaagc cgcagccgtc caaatcgcgg
     7921 cggatttgcg gcgcatcgac gccgatcagg aacacgcctt ttgccttatc tttcaacgcg
     7981 tcgcgcaggg gcgtgaagtc ctgccctttg cccatgccgc ccaaaatcac gaagagcggg
     8041 ttttgcaaac cggcaatcgc ggcggcggtc gcgccgacat tcgtgccttt gctgtcgtcg
     8101 atgaatacca cgccgttttt ctcgccgatt ttttccacac ggtgcggcaa gccttggaag
     8161 gttttgacgt gttccagcaa tgcttcgcgc ggcaaaccga cggcctcgca caaagccacg
     8221 gcagccataa cgttggcggc gttgtgcaaa ccttgcagcg ggatgtcttg cgtagaaatc
     8281 aaatcttcat tgccttgttt taaacagccc gtcccgcgtt ccaaccaaaa atcggcttcg
     8341 tgttccaagg aaaaccgttt cacttcacgc cctgcccgtt tcatggcgcg gcagaacacg
     8401 tcgtccgcat tcaaaacctg cactccatcg ccacggaaaa tcttggcttt ggtatgcgcg
     8461 tagtcgagca aatcgtcgta gcggtcgaga tggtcttcgg aaatgttcat caccgtcgcc
     8521 gcagtcgggc gcaggctttc ggtgttttcc agttggaagc tggaaagctc caacacccac
     8581 acgtccgcct ttttgccttc gcgctgcaat tctgcctcca aaaccggcgt accgatattg
     8641 cccgcaatga cggtatccag cccgcacttg atgcagagat agccgaccag gctcgttacc
     8701 gtggttttgc cgttgctgcc ggtaatcgca atcaccttgt cgccgcggcg gttcacaatg
     8761 tccgccagca attcgatgtc gcctagcacg cgcccgccgt tttgcttgaa cgcctcgata
     8821 tccggctgcc gctcgctgat gccgggactg agcgccagaa tatcgaaacc gttgtccaac
     8881 gcatctttca gacgacccgt gtaaaacacc agcccgtcaa acatcttacc gatttgcgcc
     8941 acgcgctcgg ctttcagctc cgcatcataa gcggcaacct ccgcgccgtt tttgcgcaga
     9001 taggcaatca tggaaatacc cgtgccgcca agtccggcga cgaggatttt tttgttttgg
     9061 aaagtcattt tggttttgtc ctaaaacaaa tcatattggg caggagacgt ccgcccttgc
     9121 ccaagccgct ttcagacggc atcgcgagcc gattaataac ccgccttcag gcgttggtca
     9181 ttgtcgcagc tgttttggtc tccgttttga caagccttgc caagccattg ttgagcgagc
     9241 gcaaggtctt ggcgcacgcc gcgtccatcg taatacatca agcccaaatt gtattgggct
     9301 tgggcatccc cttgttctga agccttgcga taccactgca ctgcctgtac ataatcttga
     9361 cgaactcctt gtccattttc atacatcacg cccaaattga attgggctgc tgcatttccc
     9421 tgttctgccg cctgcaagtt ttcccgaaaa tccgatacgt cacccgccca caccgcttgg
     9481 ttcaagccca aggcaatcag ggcggcggca agccatttga ctgtctgttt catggttttt
     9541 tcctgtttta gtataaggcg ggtttcagcc gccgttaacg ataggacggg gcggattgcc
     9601 gccgcaggtt tattgcgcgt tcaaatgccg tctgaaaggt gttcggacgg cataggttca
     9661 gcgaattttg agggtactca aaccgatcaa taccaagacg atggtaataa tccagaaacg
     9721 gacgacgact tgggtttctt tccagccttt ttgttcgtaa tggtgatgaa tcggcgccat
     9781 caggaagatg cgttttttgg ttttcttgta ccagccgacc tgaagcataa cggacacggc
     9841 ttctacgacg aacagaccgc ccataatgac gaggacaaat tcttggcgga cgatgacggc
     9901 aacggtaccg agcgcggcac ccaatgccag cgcgccgaca tcgcccataa agacttgcgc
     9961 gggataggcg ttgaaccaca aaaatccgag gcacgcgccg cacatggcgg tgcagaatat
    10021 agcgacttcg ttcgcgccgg cgacataggg aagctggagg tattgggaaa attggtagtg
    10081 tccgctgacg taggcgaaaa tggcgagccc ggcggcaacg aggacgacgg ggaaggcggc
    10141 aaggccgtcc aagccgtcgg tgaggttgac ggcgttggat gtgccgacga tggtcaggta
    10201 agacaacacc aaaaagccga ccacgcccag cggcagggcg atttgtttga aaaacgggac
    10261 aatcaaaata ttgttggcgg aattggcggc aaggtaaaac aatgccaaac cggcgataac
    10321 ggcaacgctt gactgccaca ccattttgaa tttggcggac acgccgttgg ggtctttata
    10381 aacgactttg cgccagtcgt cgtaaaaacc gagcgcaccg gtggcaagca gtacgcccaa
    10441 gagaatccag atatacgggt tcgcccagtt gccccacaac agggtggaca cggtaatggc
    10501 ggtcagaatc agcgaaccgc ccatcgtcgg cgtgccgttt ttgacgaggt gggtttgcgg
    10561 gccgtcggta cgcactgcct gcccgcattt gagcgcggtc agcctgcgta tcgtccacgg
    10621 gccgaacatc agggaaaacg ccaaggcggt caacgccgcc ataacggcgc ggaatgtggt
    10681 gtattgaaaa atattcagac cggttaacca gttgctgaaa tgtgcgagcc ataaaaacat
    10741 ggggcttcct tttttgtttt gtcgttatag cggat
//
LOCUS       DAXDSD010000077        10691 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXDSD010000077 DAXDSD010000000
VERSION     DAXDSD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.077"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1015
                     /gene="hupA"
                     /locus_tag="ACFB2Q_001591"
     CDS             <1..1015
                     /gene="hupA"
                     /locus_tag="ACFB2Q_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupA"
                     /protein_id="HFC0918583.1"
                     /translation="AEPHVPVSIPTATPLPAGEVTLSSDNGNIENINTAGAGSASDAP
                     SRSRRSLDAAPQNTSGISIRQREVEKDYFGYKSKETSFIFKTPGGAQYALSSYADPIT
                     VSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATSYAEITKQDYMKFGAWIGPNGEI
                     DLFAGGFPVGKTPPPAFSYGSSTPETALSKGKITYQVWGIRVRNGQFVTSSYTPPKSG
                     SYYGTLANTPVLSFITANFNSNTLAGKILGNSDYGPDVDIQNATITGPTFSGDATSGG
                     KSGKLEGKFFGKFASTRSSEVSIGGKITFDGDRSLDTVFGGVSYEKKLDDTSQDTNHL
                     TKQ"
     gene            1043..3472
                     /gene="hupB"
                     /locus_tag="ACFB2Q_001592"
     CDS             1043..3472
                     /gene="hupB"
                     /locus_tag="ACFB2Q_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC0918584.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLTNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVFDPANPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRTGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILASNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKTNPELKAEKAKN
                     WELGLAGSGKAGSFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVEGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGVNAYAARTAAKKPSDTVHSNDDLNKPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSVREFGTVNRVNNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
     gene            complement(4365..5711)
                     /gene="mnmE"
                     /locus_tag="ACFB2Q_001593"
     CDS             complement(4365..5711)
                     /gene="mnmE"
                     /locus_tag="ACFB2Q_001593"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC0918585.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EGLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            6149..10555
                     /gene="app"
                     /locus_tag="ACFB2Q_001594"
     CDS             6149..10555
                     /gene="app"
                     /locus_tag="ACFB2Q_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC0918586.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGELILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRNATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QREAETRPATTAFPRARRARRDLPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVF
                     AVQDELDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVG
                     ILFSHNRTENTFDDGIGNSARLAHGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIRGK
                     IRRRVLHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRA
                     GIKADYSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAE
                     IKGFTLSLHAAAAKGPQLEAQHSAGIKLGYRW"
BASE COUNT         2868 a         3145 c         2576 g         2102 t
ORIGIN      
        1 agccgaaccg cacgtccccg tgtccatccc caccgccacg ccgctgcccg ccggcgaggt
       61 aacgttatca agcgataacg gcaatatcga aaacatcaac accgccggcg ccggaagcgc
      121 atccgacgcg ccgagccgca gcagacgctc gctcgatgcc gccccgcaaa acacatccgg
      181 catttccatt cggcagcggg aagtagaaaa agactatttc ggctataaat caaaagaaac
      241 atcattcatc ttcaaaacac ccggcggcgc gcaatacgcc ctttcatcct acgccgaccc
      301 cataaccgtc agctactcct cccccgattt caaaattccc gaccgccacg cagggcagcg
      361 gctcgccgac ggcagccgca tctttatctg ctgcagcgac tccggggcta cttcgtatgc
      421 ggaaattacc aaacaagatt atatgaaatt cggggcgtgg ataggtccca acggcgaaat
      481 cgacctcttc gccggcggct tccccgtcgg caaaacgccg cctcctgcgt tctcctacgg
      541 cagcagcacg cccgaaaccg cattgtcaaa gggtaaaatc acttatcagg tttggggcat
      601 ccgcgtcaga aacgggcaat ttgttacttc ctcttatacg ccgccgaaaa gcggttctta
      661 ttatgggaca cttgccaata cccccgtcct ttcctttatc accgccaatt tcaacagcaa
      721 cacactggca ggcaaaatcc tcggcaacag cgattacggg ccggatgtgg atattcaaaa
      781 cgcaacgatt accggtccga ccttttccgg cgatgccacg tccggcggga aaagcggcaa
      841 attggaaggc aagttttttg gcaagttcgc cagcacccgc agcagcgaag tcagcatagg
      901 cggcaaaatc acctttgacg gcgaccgctc cctcgatacc gtattcggcg gtgtgagtta
      961 tgaaaaaaaa cttgatgaca ccagtcaaga taccaaccac cttactaaac aataaaccac
     1021 ctcatccctg caaaggtttc ttatgcccat tcccttcaaa cccgtattgg ctgccgccgc
     1081 catcgcccaa gcgtttcccg cctttgcggc agaccccgcg ccgcagtccg cccaaacgct
     1141 gaacgaaatc accgttaccg gcacgcacaa aacccaaaaa ctcggcgaag aaaaaatccg
     1201 ccgcaaaacc ttagacaagc tcttgaccaa cgacgaacac gacctggtgc gctacgaccc
     1261 cggcatttcc gtcgtcgaag gcggcagggc gggttctaac ggctttacca tacgcggcgt
     1321 ggacaaagac cgcgtcgcca tcaacgttga cgggctggcg caggcggaaa gccgctcttc
     1381 cgaagccttc caagaattgt tcggcgcgta cggcaacttc aacgccaacc gcaacacttc
     1441 cgagccggaa aacttttccg aagtaaccat caccaaaggc gcggactcgc tcaaatccgg
     1501 cagcggcgca ttgggcggcg cagtcaatta ccaaaccaaa tccgcaagcg actatgtttc
     1561 cgaagacaag ccctaccatt tggggataaa gggcggcagc gtcggcaaaa acagccaaaa
     1621 attcagcagc attaccgccg ccggcaggct ctttggtttg gatgccttat tggtttatac
     1681 ccgccgcttc ggcaaagaaa ccaaaaaccg ctcgaccgag ggcgatgtcg aaattaaaaa
     1741 cgacggatat gtctttgacc cggccaaccc ctcgcccagc agatacctta cctacaaagc
     1801 cacaggggtt gctcgctccc aacccgaccc gcaagaatgg gtaaacaaaa gcaccctgtt
     1861 caagctgggc tacaatttca acgaccgcaa ccgcatcggc tggatttttg aagactcgcg
     1921 caccgaccgt tttaccaacg agctgtctaa tttgtggacg ggtacgacca cgtctgccgc
     1981 aacgggcgac taccgccacc gccaagacgt gagctaccgc cgccgcaccg gcgtcgaata
     2041 caaaaacgaa ttggaacacg gcccgtggga cagcctcaag ctgcgctacg acaagcagcg
     2101 catcgatatg aacacttgga cttgggacat cccgaaaaat tacgatacaa acggcatcaa
     2161 cggcgaggtt taccattcgt tccggcatat ccgccaaaac accgcgcaat ggactgccga
     2221 ttttgaaaaa caactcgact tttccaaagc cgtttgggcg gcgcaatacg gcttaggcgg
     2281 cggcagaggg gacaatgcca actcggatta cagttatttc gcaaaactgt acgaccccaa
     2341 aatcctcgct tccaaccaag ccaaaatcac gatgctgatc gaaaaccggt cgaaatataa
     2401 atttgcctat tggaacaatg tgtttcactt gggcggcaac gaccgcttcc gcctgaatgc
     2461 gggcatacgc tacgacaaaa acagcagcag cgcgaaagac gatccgaaat acaccaccgc
     2521 catccggggg cagattcccc atttgggttc ggaacgcgcg cacgcgggct tcagctacgg
     2581 cacagggttc gactggcggt ttaccaagca tctgcacttg ttggcaaaat acagcaccgg
     2641 cttccgcgca ccgacttcgg acgaaacctg gctgctgttc ccacaccccg atttctacct
     2701 gaaaaccaat cccgaactga aagcggaaaa agccaaaaac tgggaattgg gcctggcggg
     2761 cagcggcaaa gctggcagct tcaagctttc gggcttcaaa accaaatacc gcgactttat
     2821 cgaattgacg tatatgggcg tttcgtcaga cgataaaaac aaccccagat acgccccgct
     2881 ttcagacggc acggcattgg tcagctcgcc cgtttggcaa aaccaaaacc gcaccgccgc
     2941 ctgggtggaa ggcatagagt ttaacggcac ttggaacctc gacagcatcg ggctgcccaa
     3001 agggctgcac accggcctca acgtcagcta catcaagggc aaggcaacgc aaaacaacgg
     3061 caaagaaacg cccatcaacg cgctttcgcc gtggacggcg gtttacagcc tgggctatga
     3121 cgcgccttcc aaacgctggg gcgtcaacgc ctacgccgcg cgcaccgccg ccaaaaagcc
     3181 gtccgacacc gtccacagca acgacgactt gaacaaaccg tggccttatg ccaaacacag
     3241 caaggcctat acgctgttcg acctttccgc ctacctcaac atcggcaaac aggttacgct
     3301 ccgcgccgcc gcgtacaaca ttaccaacaa gcagtactac acttgggaat ctttacgcag
     3361 cgtccgcgag ttcggcacgg tcaaccgcgt caacaacaaa acccacgccg gcatccaacg
     3421 ctttacctcg ccgggcagga gctacaattt caccatcgaa gcgaagttct aagctcctga
     3481 tgtgccaatg ccgtctgaaa cccgatgatt tgggtttcag acggcatttt ttacggtcgc
     3541 accgccgccc cctttcaggc ttgtaccaag cccaatcccg caactctccg tcattcccgc
     3601 aactctccgt cattcccagg gaagtgggaa tctagaaacg caaagccaca ggaacttatc
     3661 cgaaacaaca gcaacctaaa attcgtcatt cccacgaaag tgggaatcca gttcgttgag
     3721 tttcagttat ttaaaataaa ttttgaaact ttaatcccat cattcccgcg aaagcgggaa
     3781 tccggaacgt aaaatctaaa gaaaccgttt tatccgataa gtttccgtac cgacggggct
     3841 ggattcccgc ctgcgcggga atgacggcgg agcggtttct gttttttccg ataaattcct
     3901 aaaacttaaa atttcatcat tcccacaagg acagaaaacc aaaaacagaa acctaaaatt
     3961 cgtcattccc gcaaaagcgg gaatccagtt cgttgagttt cagttattta gaataaattt
     4021 tgaaacttta atcccgtcat tcccgcgaaa gcgggaatcc ggaacgtaaa atctaaagaa
     4081 accgttttcc cgataagttt ccgtgccgac ggggctggat tcccgcctgc gcgggaatga
     4141 cgatatttca gttttctgtt tttgattttt ttgtttttac aggaatgacg gggctttcag
     4201 tattgaaaaa agttgccgta ccgcgccgat aatttccgcc tgcgcggaat gaagatttaa
     4261 gcgttgcccg aaattcaaaa aaccaaaacc gaacggatcg gattcccgtt tttatgggat
     4321 aacggaatgt tcagccggac accgccacaa tgcctccggc ccgcttattt tccgatgcaa
     4381 aacctcgaaa aaatcacgcc gagcaggtcg tccgccgtaa attcccccgt aatttcgccg
     4441 cacgcgacct gagccagccg caagtgttcg gcaaacagct cgatttgatg gttgccgcac
     4501 aaagcggcca gcgacaattc ttcctgcgcc gccttgagtg cgttgacgtg ccgcgtccgc
     4561 gccaaaaaca gcccttcgct ttcgccctgc caaccggcct cgcacaacag cgtccgtttc
     4621 agcgcgtcca agccgtcgcc ggttttcgcc gacagcgcga tgacggtttc cgcgcccgta
     4681 ccgaacccgc ctgccgcgtg tgcgtgcaaa tcggatttgc tgtggatttc aatgcgtttc
     4741 aaatccgacg gcaacgtatc caaaatcatc cgcgtctttt cattcaaacc ctcgcgcgga
     4801 tcgaccaaca ccagcgcgac atcggcttcg gatacggctt tgcggctgcg ttcgatgccg
     4861 atgcgttcga ccacgtcgtc cgtctcgcgc aaacctgccg tatcgacaat atgcaccggc
     4921 acgccgtcaa tcaggatacg ttccctgacc gcgtcgcgcg tcgttccggc aatatcggta
     4981 acaatcgcca cttcgtcgcc cgccagcgcg ttcaacaggc tggacttgcc gacattcggc
     5041 gcaccgacca atacgacatt cagaccttcg cgtaaaatcg cgccctgctg cgcgttggca
     5101 agcacatcat ccacggcgcg gcgcaagccg tccagtttgc cgcgtgcgtc cgccgcttcg
     5161 agaaaatcga tgtcttcctc gggaaaatcc agcgccgctt cgaccagcat ccgcaaggta
     5221 atcaagcctt cgaccaaacc gtgtatccgc cgcgaaaaat cgcccttgag cgagcgcaac
     5281 gccagacgcg ccgccgaacg gccggatgcg tcaatcaaat ccgccacgcc ttccgcctgt
     5341 gccaagtcca gtttgtcgtt taaaaacgca cgtttggtaa actcgcccgg ctcggcaagg
     5401 cgcgcgccca gttccaaaca gcggttcagc agcatctcca tcactaccgg cccgccgtgt
     5461 ccctgaagct cgatgacatc ttcgcccgta aaacttgccg gcgcggcaaa aaacagtagc
     5521 agcccgctat cgattgcctg tccgtccgcg tccgtaaaat cagcataggt tgccacgcgc
     5581 ggctcgggcg ttttcccaca caaagcctgc gccatcggca acaggttttt ccccgatatg
     5641 cgtatcacgc ccacgccgcc gcgtccgggc gcggtcgcga ctgccgcaat cgttggaacg
     5701 ttatccgaca taaaaccccc gaaaattcaa aacagccgcg attatagcaa atgccgtctg
     5761 aagtccgacg gttcggcttt cagatggcat tgccggcttg tgtaaaacga tattccgaca
     5821 ccgcacggca caaaaccctg ccccgcattc cgcctccggt cggtttttac cgccgaaaac
     5881 ggcggggttc cgaccgaaaa ggaacgggga taaagtaaag tgtgatatga tttcgtattc
     5941 aaaatgatgc ctttatgtgc aacccatcga ttgctccggc gagttgataa aaatcccctt
     6001 tttttccttc ccgcccttcc ctgcaccatc tccgaacaga agcggcaaaa tacgccgaac
     6061 cgcacatata gccacatccc gcgcgccgcc cggaagcgga agccgcgccc tcccaaacaa
     6121 accgaatccc gtcagataag gaaaaataat gaaaacaacc gacaaacgga caaccgaaac
     6181 acaccgcaaa gcccctaaaa ccggccgcat ccgcttctcg cccgcttact tagccatatg
     6241 cctgtcgttc ggcattctgc cccaagcccg ggcgggacac acttatttcg gcatcaacta
     6301 ccaatactat cgcgactttg ccgaaaataa aggcaagttt gcagtcgggg cgaaagatat
     6361 tgaggtttac aacaaaaaag gggagttggt cggcaaatcg atgacgaaag ccccgatgat
     6421 tgatttttct gtggtatcgc gtaacggcgt ggcggcattg gcgggcgatc aatatattgt
     6481 gagcgtggca cataacggcg gctataacaa tgttgatttt ggtgcggagg gaagcaatcc
     6541 cgatcagcac cgcttttctt accaaattgt gaaaagaaat aattataaag cagggactaa
     6601 cggccatcct tatggcggcg attatcatat gccgcgtttg cacaaatttg tcacagatgc
     6661 agaacctgtt gagatgacca gttatatgga tgggtggaaa tacgctgatt taaataaata
     6721 ccctgatcgt gttcgaatcg gagcaggcag acaatattgg cggtctgatg aagacgaacc
     6781 caataaccgc gaaagttcat atcatattgc aagcgcatat tcttggctcg tcggtggcaa
     6841 tacctttgca caaaatggat caggtggtgg cacagtcaac ttaggtagcg aaaaaattaa
     6901 acatagccca tatggttttt taccaacagg aggctcattt ggcgacagtg gctcaccaat
     6961 gtttatctat gatgcccaaa agcaaaagtg gttaattaat ggggtattgc aaacaggcaa
     7021 cccctatata ggaaaaagca atggcttcca gctagttcgt aaagattggt tctatgatga
     7081 aatctttgct ggagataccc attcagtatt ctacgaacca catcaaaatg ggaaatactt
     7141 ttttaacgac aataataatg gcgcaggaaa aatcgatgcc aaacataaac actattctct
     7201 accttataga ttaaaaacac gaaccgttca attgtttaat gtttctttat ccgagacagc
     7261 aagagaacct gtttatcatg ctgcaggtgg ggtcaacagt tatcgaccca gactgaataa
     7321 tggagaaaat atttccttta ttgacaaagg aaaaggtgaa ttgatactta ccagcaacat
     7381 caaccaaggc gcgggcggtt tgtattttga gggtaatttt acggtctcgc ctaaaaacaa
     7441 cgaaacgtgg caaggcgcgg gcgttcatat cagtgatggc agtaccgtta cttggaaagt
     7501 aaacggcgtg gcaaacgacc gcctgtccaa aatcggcaaa ggcacgctgc tggttcaagc
     7561 caaaggggaa aaccaaggct cggtcagcgt gggcgacggt aaagtcatct tagatcagca
     7621 ggcggacgat caaggcaaaa aacaagcctt tagtgaaatc ggcttggtca gcggcagggg
     7681 gacggtgcaa ctgaatgccg ataatcagtt caaccccgac aaactctatt tcggctttcg
     7741 cggcggacgt ttggatttga acgggcattc gctttcgttc caccgcattc aaaataccga
     7801 tgaaggggcg atgattgtca accacaatca agacaaagaa tccaccgtta ccattacagg
     7861 caataaagat attactacaa ccggcaataa caacaacttg gatagcaaaa aagaaattgc
     7921 ctacaacggt tggtttggcg agaaagatgc aaccaaaacg aacgggcggc tcaatctgaa
     7981 ttaccaaccg gaagaagcgg atcgcacttt actgctttcc ggcggaacaa atttaaacgg
     8041 caatatcacg caaacaaacg gcaaactgtt tttcagcggc agaccgacac cgcacgccta
     8101 caatcattta ggaagcgggt ggtcaaaaat ggaaggtatc ccacaaggag aaatcgtgtg
     8161 ggacaacgat tggatcgacc gcacatttaa agcggaaaac ttccatattc agggcggaca
     8221 agcggtggtt tcccgcaatg ttgccaaagt ggaaggcgat tggcatttaa gcaatcacgc
     8281 ccaagcagtt ttcggtgtcg caccgcatca aagccacaca atctgtacac gttcggactg
     8341 gacgggtctg acaagttgta ccgaaaaaac cattaccgac gataaagtga ttgcttcatt
     8401 gagcaagacc gacatcagag gcaatgtcag ccttgccgat cacgctcatt taaatctcac
     8461 aggacttgcc acactcaacg gcaatcttag tgcaggcgga gacacgcact atacggttac
     8521 gcgcaacgcc acccaaaacg gcaaccttag cctcgtgggc aatgcccaag caacatttaa
     8581 tcaagccaca ttaaacggca acacatcggc ttcggacaat gcttcattta atctaagcaa
     8641 caacgccgta caaaacggca gtctgacgct ttccgacaac gctaaggcaa acgtaagcca
     8701 ttccgcactc aacggcaatg tctccctagc cgataaggca gtattccatt ttgaaaacag
     8761 ccgctttacc ggaaaaatca gcggcggcaa ggatacggca ttacacttaa aagacagcga
     8821 atggacgctg ccgtcgggca cggaattagg caatttaaac cttgacaacg ccaccattac
     8881 actcaattcc gcctatcgac acgatgcggc aggcgcgcaa accggcagtg cggcagatgc
     8941 gccgcgccgc cgttcgcgcc gttccctatt atccgttacg ccgccaactt cggcagaatc
     9001 ccgtttcaac acgctgacgg taaacggcaa attgaacggt cagggaacat tccgctttat
     9061 gtcggaactc ttcggctacc gcagcggcaa attgaagctg gcggaaagtt ccgaaggcac
     9121 ttacaccttg gctgtcaaca ataccggcaa cgaacccgta agtctcgagc aattgacggt
     9181 agtggaagga aaagacaaca caccgctgtc cgaaaatctt aatttcaccc tgcaaaacga
     9241 acacgtcgat gccggcgcat ggcgttatca gcttatccgc aaagacggcg agttccgcct
     9301 gcataatccg gtcaaagaac aagagctttc cgacaaactc ggcaaggcgg gagaaacaga
     9361 ggccgccttg acggcaaaac aggcacaact tgccgccaaa caacaggcgg aaaaagacaa
     9421 cgcgcaaagc cttgacgcgc tgattgcggc cgggcgcaat gccaccgaaa aggcagaaag
     9481 cgttgccgaa ccggcccggc aggcaggcgg ggaaaatgcc ggcattatgc aggcggagga
     9541 agagaaaaaa cgggtgcagg cggataaaga caccgccttg gcgaaacagc gcgaagcgga
     9601 aacccggccg gctaccaccg ccttcccccg cgcccgccgc gcccgccggg atttgccgca
     9661 accgcagccc caaccgcaac cccaaccgca gcgcgacctg atcagccgtt atgccaatag
     9721 cggtttgagt gaattttccg ccacgctcaa cagcgttttc gccgtacagg acgaattgga
     9781 ccgcgtgttt gccgaagacc gccgcaacgc cgtttggaca agcggcatcc gggacaccaa
     9841 acactaccgt tcgcaagatt tccgcgccta ccgccaacaa accgacctgc gccaaatcgg
     9901 tatgcagaaa aacctcggca gcgggcgcgt cggcatcctg ttttcgcaca accggaccga
     9961 aaacaccttc gacgacggca tcggcaactc ggcacggctt gcccacggtg ccgttttcgg
    10021 gcaatacggc atcggcaggt tcgacatcgg catcagcgcg ggcgcgggtt ttagtagcgg
    10081 cagcctttca gacggcatca gaggcaaaat ccgccgccgc gtgctgcatt acggcattca
    10141 ggcaagatac cgcgcaggtt tcggcggatt cggcatcgaa ccgcacatcg gcgcaacgcg
    10201 ctatttcgtc caaaaagcgg attaccgata cgaaaacgtc aatatcgcca ccccgggcct
    10261 tgcattcaac cgctaccgcg cgggcattaa ggcagattat tcattcaaac cggcgcaaca
    10321 catttccatc acgccttatt tgagcctgtc ctataccgat gccgcttccg gcaaagtccg
    10381 aacgcgcgtc aataccgccg tattggcgca ggatttcggc aaaacccgca gtgcggaatg
    10441 gggcgtaaac gccgaaatca aaggtttcac gctgtccctc cacgctgccg ccgccaaggg
    10501 gccgcaattg gaagcgcagc acagcgcggg catcaaatta ggctaccgct ggtaaccgcc
    10561 ggatatgccg aaaggggttt gacgatgccg ccgtgcactg tcaaacccct ttttctgcca
    10621 ccttgtgcct actccatcgt ctgataaccg tcaaattgat ttttagccca tgttcggcga
    10681 gtcggggaaa t
//
LOCUS       DAXDSD010000078        10508 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXDSD010000078 DAXDSD010000000
VERSION     DAXDSD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.078"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(67..1305)
                     /locus_tag="ACFB2Q_001595"
                     /pseudo
     CDS             complement(67..1305)
                     /locus_tag="ACFB2Q_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(1485..2858)
                     /locus_tag="ACFB2Q_001596"
     CDS             complement(1485..2858)
                     /locus_tag="ACFB2Q_001596"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC0918587.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3072..4074
                     /gene="dusB"
                     /locus_tag="ACFB2Q_001597"
                     /pseudo
     CDS             3072..4074
                     /gene="dusB"
                     /locus_tag="ACFB2Q_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
     gene            4087..4236
                     /locus_tag="ACFB2Q_001598"
     CDS             4087..4236
                     /locus_tag="ACFB2Q_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918588.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4263..4502
                     /locus_tag="ACFB2Q_001599"
     CDS             4263..4502
                     /locus_tag="ACFB2Q_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC0918589.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4505..5041
                     /gene="ruvC"
                     /locus_tag="ACFB2Q_001600"
     CDS             4505..5041
                     /gene="ruvC"
                     /locus_tag="ACFB2Q_001600"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC0918590.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5092..5961
                     /locus_tag="ACFB2Q_001601"
     CDS             5092..5961
                     /locus_tag="ACFB2Q_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFC0918591.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            6615..6749
                     /locus_tag="ACFB2Q_001602"
     CDS             6615..6749
                     /locus_tag="ACFB2Q_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918592.1"
                     /translation="MPSERIFRSVLRLVGVSSDTVKRKFAGAGLSYAGKAAGESLYCL
                     "
     gene            6865..7659
                     /locus_tag="ACFB2Q_001603"
     CDS             6865..7659
                     /locus_tag="ACFB2Q_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC0918593.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            7884..10487
                     /gene="pepN"
                     /locus_tag="ACFB2Q_001604"
     CDS             7884..10487
                     /gene="pepN"
                     /locus_tag="ACFB2Q_001604"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC0918594.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLMT
                     EAEQAFPLEGVTEAVVPSLLRGFSAPVYLNYPYSDDDLLLLLAHDSDAFTCWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELDRQAAKQENQSYEYSPETADWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
BASE COUNT         2409 a         2746 c         2985 g         2368 t
ORIGIN      
        1 tgaatgatga aataaaaatg ccgtctgaac ccgattggat tcagacggca ttttgtatgg
       61 caggatttat tcgctttcca actgaccgac ccattctgac aaggcagtca ggcgttgttc
      121 ggatttcgcc acgaacgggt tttcggtatc ccacgcgtag cctgccaaaa tcgaagaaag
      181 catacggctg atttccgggc tgcggtcggg cgcgtacacg acgttttgga agcggaaatc
      241 ataccagccg tccacatagg tgcggaacgc gtctacgccg atcatcaggg gttcggcaaa
      301 ttcggtttgc caatcggcgg cttcgctttt gagttgtttt gtcagcagat cggcagcaag
      361 ttcggcggag tgcagcgcga tggttacgcc cgacgagaac acggggtcga ggaactcggc
      421 ggcattgccc aacagcgcga aatgcctgcc gtgcagtgat ttgacgttgg cggaatagcc
      481 ttggatggag cggaacggaa aatcgttttc ccaaacggct ttgtccaaaa tttcgcttaa
      541 catcgggcat tcgtaaacaa attttttcaa caccgtttcc gattcgccgg caagtttgtc
      601 gggtgtgccg accacgccga cggaacagcg gttgtcgccg aagggaatca gccaaatcca
      661 cacgtcgcgg ttgttgcgga tgggtggtaa tcaggatttt gttgcggtcg aatttcgggt
      721 gggtaatgtt gtcgtcgatg tgcgtgaaat gcgtttggcg cggcggcagg tgcgagggcg
      781 tttccaagtt tagcaggcgc ggcagcacgc gtccgtagcc gcttgcgtcc aagacgaatt
      841 tcgcggtcag ttcatagctc tcgccggtgt cggtttcgat gttcaagcgg gcaaaatcgc
      901 cgctgttgtc gaacgcggtt acgccgtgcc cgaaacgtac ttcaacgcct tgtttggcgg
      961 cttcttcaat caggattttg tcgaacacgg cgcggcggac ttggtaaacc gtgccggggc
     1021 cgtctgaaaa tttatcggtg aaatcaaact cggtatagcg gctgccccac gaaaacgccg
     1081 caccgttttt caactgaaag ccgggcccgg tgcgaacggc atcggcaaaa ccggcttctt
     1141 ccagcatttc catacagtgc ggcagcaggc tttcgccgat gacgaagcgc ggaaagtgct
     1201 gttttccaac acgcagactt gataaccttt tttgcggagc agggcggatg cggccgaacc
     1261 tgccggacct gcgccgatta cggcgacatc gaattgggtg gacattggga aatcctgatg
     1321 gtttgagtgg aaaaggaaat gatattgtac aggcagcaaa tgccgtctga aactgtttgc
     1381 ggaagaaatt tatccggtgc gggcggttgc tcccaatgcg tcagtccggt ttgaaaaatg
     1441 ccgtctgaaa cgggaaatgt tcagacggca tttgattttc agggttattt tacgccgtaa
     1501 cgcggttgga gcagggcgca cgtccggtgt ccccggcggc ttcggcggct gcgcccggca
     1561 atttttccgc aggccgcgcc cgcatcgttt gccgtggttt ccggccgggt atttttttcg
     1621 cgctgagccc tggcggattc gtagcggttg cgttgtctcg gttcgcttgt ttcggttttt
     1681 tccggttttg agccttcctg ttcccaccat cggggctcga agccttcgat gcgttcgatg
     1741 agcagcttgt tgccggtcag ttctttgatg gcctcaaaca ttttctgttc ggattcgtcc
     1801 atcagggaaa tcgccacgcc gtccgcaccg gcgcgccccg tgcgtccgat gcggtggatg
     1861 tagtcttcgg gctgggcggg catttcgtaa ttgatgacga agggcagttc ggcgatgtcc
     1921 agcccacgcg cggcgatatc ggtggcgacg aggacgcgca ggctgccgtc tttgaaggcg
     1981 ttgagtgttt cgagcctgct ttgttgggaa cggtcgccgt gtatcgcctg tgcggacagg
     2041 ttgcggcgca ccagttcgcg cgttacgcgg tcgacgcttt gtttggtttt gcaaaagacg
     2101 ataacttggt tcatatgcag atcgacaatc agccgttcga gcaggttgcg cttttggaag
     2161 gtatcgacgg cgatgatgtg ttgttcgacg ttggcgttgg tggtgttttg ggcggcaacc
     2221 tcgacggttt cgggcgcgtt catgaagtct tgcgccagtt tgcgtatcgg ggcggagaag
     2281 gtggcggaaa agagcagggt ttggcgttgg cggggcagca tctgcatgat tttgcggatg
     2341 tcgtcgataa accccatatc caacatgcgg tctgcttcgt ccagaacgac gatttcggct
     2401 ttgtttaaac tgatgttttt ctgtttcaca tggtcgagca gccgtccgac ggtggcgacg
     2461 actatttcgc agccggcacg caggtcggcg gtttgtttgt ccatgttgac accgccgaag
     2521 aggacggtat gccgcagcgg caggttttta atgtagccct gcacgttttg gtcgatttgg
     2581 tcggcaagtt cgcgcgtcgg ggtcaacacg agcatacgca cggggtgcat tgcaggcgag
     2641 gtgctggcgg tggcgtaacg tttgaggcgt tccagactgg gcagcataaa ggcagcggtt
     2701 ttgcctgtgc cggtttgcgc ggcggcaagc aggtcgtgtc cggccagtgc tttgggaatg
     2761 gccgcggctt ggatgggcgt cgggttttcg taaccttgcg cggtcagtgc ggaaaccagt
     2821 tccgtaccca aacctaaaga ggaaaatgga ttactcatga ttgtagtctt tctttcagac
     2881 cttatgccgt ctgaagcggg aaaccgatag gacggggaaa taaactgccc gcctgtgaac
     2941 ggcggggacg ggaatttgtg tgcggattat gccataaaac ggtgcccgcc ccaatcgcgg
     3001 gcgcgtccgc agattggaaa tcctgcttaa aaaatgtaca atggcgcact tttttgaaac
     3061 gcgggcccat tatgcacatc ggcggttatt ttatcgacaa ccccatcgca cttgcgccga
     3121 tggcgggcat taccgacaaa cccttccgcc gcctctgtcg ggcgtttggc gcaggttggg
     3181 cggtgtgcga aatgctggcc agcgatccga cgctcaggaa taccggaaaa accctgcacc
     3241 gcagtgattt tgccgatgaa ggcggcatcg ttgccgtgca gattgccggc agcgaccccg
     3301 aacagatggc ggatgcggcg cgttacaacg tcggactcgg ggcgcaggtc atcgacatca
     3361 atatgggctg ccccgccaag aaagtgtgca acgtccaagc cggtagcgcg ctgatgcagg
     3421 acgagccgct ggttgccgcc attttggagg cggtggtcaa ggcggcgggc gtacccgtta
     3481 ccctcaaaac ccgtttgggt tggcacgacg accatcaaaa cctgcccgcc atcgccaaaa
     3541 tcgccgaaga ttgcggcatt gccgcccttg ccgtccacgg gcgcgcgcgc acgcaaatgt
     3601 acaaaggcga ggcgcgttac gaactcatcg ccgagaccaa aagccgtctg aacatcccgg
     3661 tctgggtcaa cggcgacatc acttcgccgc aaaaagccgc cgccgtcctc aaacaaaccg
     3721 ccgccgacgg catcatgata gggcgcggcg cgcaaggcag gccgtggttt ttccgcgatt
     3781 tgaagcatta tgccgaacac ggcgttttac cgcctgcctt gagtttggca gaatgcagag
     3841 ccgccatttt gaaccacatc cgcgccatgc acgcgtttta tggtgagacc gtcggtgcgc
     3901 atcgcacgca aacacatagg ctggtacatc ggcgaaatgc ccgacggcga acaggcgcgg
     3961 cgtgaaatca accgcttgga caatgcggcg gcgcaatacg acacacttgc cggttatctt
     4021 gaaaggcttg ccggaaaaac cgaccgttgg gcgtgcggct atcgggaagg gtagggcagt
     4081 attgccatgc cgccgttcgg gttcggacgg catctgtctg catggttcgg aggtcgggcg
     4141 gaatccaggc cggcgaatgt cgaaaacaat aaatgccgtc tgaaaacgag tggaacgggt
     4201 ttcgccaaaa ggctttcaga cggcattccg tcttaaaaac atttcaatca aaaggatatg
     4261 cgatgaaccc tgcgactgcg gacattgcgc aatgtatcga gcagaacttg aaccaatatt
     4321 tcaaagacct gaacggcacc gaaccttgcg gcgtgtacga tatggtactg catcaggtgg
     4381 aaaagccgct gctggtgtgc gtgatggagc aatgcggcgg caaccagtcc aaagcctccg
     4441 tgatgctggg actgaaccgc aataccttgc gtaagaaact gattcaacac ggtttgctgt
     4501 gaatatgtcg gcaaccgtcc gtatcttggg catcgacccg ggcagccgcg taacgggttt
     4561 cggcatcatc gatgtcaggg ggcgcgatca tttttacgtc gcctccggct gcatcaaaac
     4621 gcctgccgat gagcctctgg cagacaggat tgccgtgatt gtgcggcata tcggcgaagt
     4681 cgttgccgtt tacaagccgc aacaggcggc ggtggaacag gtgttcgtca acgtcaatcc
     4741 ggcatcgacg ctgatgctcg gtcaggcgcg cggagcggca ttggcggctt tggtcagcca
     4801 taagctgccc gtttcggaat acacggcctt gcaggtcaaa caggcggtgg tcggcaaagg
     4861 caaggcggcg aaagaacagg tgcagcatat ggtggtgcaa atgctgggac tttcgggaac
     4921 gccgcaggcg gatgcggcgg acggtcttgc cgtcgcgctg acccacgcct tacgcaacca
     4981 cgggcttgcc gccaaactca atccttcggg gatgcaggtc aagcgcggaa ggtttcaata
     5041 gtttcagacg gcatttgtat tttgccgcct gaaaagaaaa tgtgtaccga gatgaaattt
     5101 atattttttg tactgtatgt tttgcagttt ctgccgtttg cgctgctgca caagattgcc
     5161 ggcctgatcg gttcgcttgc ctaccttctg gtcaaaccgc gccgccgtat cggcgaaatc
     5221 aatttggcaa aatgttttcc cgaatgggac gaagaaaagc gtaaaaccgt gttgaaacag
     5281 catttcaaac acatggcaaa actgatgctc gaatacggct tatattggta cgcgcctgcc
     5341 aaatgcctga aatcgctggt gcgctaccgc aataagcatt atttggacga cgcgctggcg
     5401 gcgggggaaa aagtcatcat cctgtacccg cactttaccg cgttcgagat ggcggtgtac
     5461 gcgcttaatc aggatgtccc gctgatcagt atgtattccc accaaaaaaa caagatattg
     5521 gacgaacaga ttttgaaagg ccgcaaccgc tatcacaacg tcttccttat cgggcgcacc
     5581 gaagggctgc gcgccctcgt caaacagttc cgcaaaagca gtgcgccgtt cctgtatctg
     5641 cccgatcagg atttcggacg caacaattcg gtttttgtgg attttttcgg cattcagacg
     5701 gcaacgatta ccggcttgag ccgcattgcc gcgcttgcaa atgcaaaagt gatacccgcc
     5761 attcccgtcc gcgaggcgga caatacggtt acattgcaat tctatcccgc ttggaaatcc
     5821 tttccgagtg aagacgcgca agccgacgcg caacgtatga accgctttat cgaagaacgc
     5881 gtgcgcgaac atccggaaca atatttctgg ctgcacaagc gtttcaaaac ccgtccggaa
     5941 ggcagccccg atttttactg actacataaa attacaaaac aaatcaggcg tttcagatca
     6001 aaaaccccga ttgtttttgg gaatttgaaa cccgggttgt acaaacagga tttgccggac
     6061 ggttttaacg gttcagttgt ttgtaaaaac aatgcttttt taaaattgac aaaaaacgaa
     6121 atcggtttta aaggcttatt ccgagaacaa aggggagtgg atgccgaaaa cccggttaat
     6181 atattatagt ggattaacaa aaaccaatac ggcgttgctt cgccttagct caaagagaac
     6241 gattccctaa ggtgctgaag caccaggcga atcggttccg tactatttgt actgtctgcg
     6301 gcttcgccgc cttgtcctga tttttgttaa tccactataa aattaaattt gtttaaaaac
     6361 ataaagttgt aaacaagtat ctcatataag cctttttcat taaacagata gtcagatatt
     6421 ttgtgctaaa aatttatata atatttaaat taatatcaag ttataaaaaa tatatggaat
     6481 tttattttgt ttatttataa ttttaagcaa taatctaaat caggcatttt tattcccctg
     6541 ttttaaaaaa tatttggcaa ggtgtgaaaa aaggcgtaca ttccgctaca cagaattaca
     6601 gatacagcgg agcaatgccg tctgaaagga ttttccggtc agtcttgcgg ttggtcgggg
     6661 tttcatcgga tacggtgaaa cgaaagtttg ccggcgcagg gttgagctac gcgggtaaag
     6721 ccgcaggcga aagcctgtat tgtttgtgaa gcgtaaatct ctgatttgag gtattggggc
     6781 aatcctgtgg gggttgcctc tttttttatc cgccttttaa tgacacaata ggcgcaagtc
     6841 tgtttttgaa tgcaaggtac tgccatgaat acgattttgg ctttcgatat tgaaaccgta
     6901 cccgatgtgc agggtatccg tacgttgtat gacttgccgt cttccctgcc ggatgacgaa
     6961 gtggtgctgt tcgcgcagca gaaacgccgc gcacagaccg gcggcgattt tatgcagcac
     7021 cacctccatc aggttgtggc ggtttcctgc tgtatgcgct gggggcagga caaggttcat
     7081 gtcggcacca tcggcgagat ggacgatggc gaagaagtcg tgattgccaa gtttttcgaa
     7141 ttggttgaaa aacatacgcc gcaactggtc agctggaacg gcggcggttt cgacctgccc
     7201 gtactgcatt accgctccct gatatacggc atcaacgccg cgcgctattg ggatatgggc
     7261 gacggcgatt tcggcgacag ccgcgatttc aagtggaaca actacatcag ccgttatcac
     7321 caacgccact gcgatttgat ggatttgctc gcgctttacc agccgagggc gaatgtgccg
     7381 ctggacgata tggcgaaact gtgcggcttt ccgggcaagc tgggtatgga cggcagcaag
     7441 gtgtgggagg cgttccacgc gggcaggctg aaggaaatcc gcaattattg cgaaaccgat
     7501 gccgtgaata cgtatttgat gtatctgcgt ttctgtctgg tcagcggcag attcgacgcg
     7561 gacgaatacg aaatggaaat caagcggatc agaaactatc tctccgccca aacagaagac
     7621 aaaccgcatt gggcagaatt tgtccaagcg tggaaataga aacctgctgt ccgaaggcgg
     7681 tacgcttgtg ccgccgtttc ggtttcagac ggcgtatggt ggattaaatt caaaccggta
     7741 cagccttgcc tctccttgcc ttactatctg tactgtctgc ggcttcgtcg ccttgtcctg
     7801 atttttgtta atccgcttta aaagctgtat ccgtgccgtc tgaagccttt tccagtcaaa
     7861 ccaaacaata ttgaaagaaa aaaatgagca aaaccgtccg ttatctgaaa gattaccaaa
     7921 cgcctgccta ccgcattctt gaaaccgaac tgcatttcga cattgccgaa ccgcaaaccg
     7981 tcgtgaagtc gcgtttgacg gtcgagccgc agagggcggg cgagccgctg gtgttggacg
     8041 gttcggcaaa actcttgtcc gtcaaaatca acggcgcggc ggcggattat gtgttggaag
     8101 gcgagacgct gacgattgca gacgtaccgt ccgaacgctt caccgtcgaa gtggaaaccg
     8161 aaatcctgcc ggcggaaaac aaatcgctga tggggctgta tgcttccggc ggcaatctgt
     8221 ttacccagtg cgagccggag ggcttccgca aaatcacgtt ctacatcgac cgtccggatg
     8281 tgatgtccaa gttcacgacc accatcgtcg cggacaaaaa acgctatccc gttttgcttt
     8341 ccaacggcaa caaaatcgac ggcggcgagt tttcagacgg ccgccattgg gtgaaatggg
     8401 aagacccgtt tgccaaaccg agttatctgt ttgctttggt cgcgggcgat ttggcggtaa
     8461 cggaagaccg tttcaccacc atgagcggca gaaacgtcaa aatcgagttt tacaccaccg
     8521 aagcggacaa acccaaggtc ggctttgccg tggaatcgtt gaaaaacgcg atgaagtggg
     8581 acgaaacgcg cttcgggttg gaatatgact tggatatttt catggtcgtc gccgtaggcg
     8641 atttcaatat gggcgcgatg gaaaacaagg gtttgaacat ttttaacacc aagttcgtcc
     8701 tcgccgacag ccgcaccgcc accgataccg atttcgaagg cattgaatcc gtggtcggac
     8761 acgaatattt ccacaactgg acgggcaacc gcgtaacctg ccgcgactgg ttccagcttt
     8821 cgctgaagga agggctgacc gtgttccgcg accaagagtt ttccggcgac cgcgccggcc
     8881 gcgccgtgcg ccgcatcgag aacatccgcc tgctgcgcca gaaccagttc cccgaagacg
     8941 caggcccgac cgcccatccg gtgcgccccg tcagctatga ggagatgaac aatttctaca
     9001 ccatgaccgt ttatgaaaaa ggcgcggaag tggtgcggat gtatcatacc ctgctcggcg
     9061 aagagggctt ccaaaaaggc atgaagctat atttccaacg ccacgacgga caggcagtga
     9121 cctgcgacga tttccgcgcg gcgatggcgg atgcgaacgg catcaatctc gaccagttcg
     9181 ccttgtggta cagccaggcg ggcacgcccg ttttggaagc cgaaggccgt ctgaaaaaca
     9241 atgttttcga gttaaccatt aaacaaaccg tgccgcccac gcccgatatg gcggacaaac
     9301 agccgatgat gattcccgtc aaagtcgggc ttctgaaccg caacggcgaa gcggtggcat
     9361 tcgattatca gggcaaacgc gcaaccgaag ccgtgttgct gatgaccgaa gccgaacagg
     9421 ccttcccgct cgaaggtgta accgaagccg tcgttccctc gctgctgcgc gggttcagcg
     9481 cgccggtgta tctgaactat ccgtacagcg acgacgacct gctgctcctg ctcgcccacg
     9541 acagcgacgc tttcacgtgc tgggaagccg cccaaacgct ctaccgtcgc gccgtcgccg
     9601 ccaaccttgc cgcgctttca gacggcatcg ggttgccgaa acacgaaaaa ctgcttgccg
     9661 ccgtcgaaaa agtcatttca gacgacctct tggacaacgc cttcaaagcc ctgcttttgg
     9721 gcgtgccgtc cgaagccgaa ctgtgggacg gcacggaaaa catcgacccg ctgcgctacc
     9781 atcaggcgcg cgaagccttg ttggatacgc ttgccgtccg cttcctgccg aaatggcacg
     9841 aattggaccg tcaggcggcg aagcaggaaa accaaagtta cgaatacagc cccgaaaccg
     9901 ccgactggcg cacgctgcgc aacgtctgcc gcgccttcgt cctgcgcgcc gaccccgcgc
     9961 acatcgaaac tgttgccgaa aaatacggcg aaatggcgca aaacatgacc cacgaatggg
    10021 gcatcctgtc cgccgtcaac ggcaacgaaa gcgatacgcg caactgcctg ctggcgcagt
    10081 ttgccgacaa gttttcagac gacgcgctgg tgatggacaa atatttcgcc cttatcggct
    10141 caagccgccg cagcgacacc ctgcaacagg ttcaaaccgc cttgcagcat ccgaaattca
    10201 gtctcgaaaa ccccaacaaa gcccgttcgc tcatcggcag cttcagccgc aacgtcccgc
    10261 attttcacgc acaagacggc agcggctacc gcttcatcgc cgacaaagtc atcgaaatcg
    10321 accgcttcaa cccgcaggtc gccgcccgcc tggtgcaggc gttcaacctc tgcaacaagc
    10381 tcgagccgca ccgcaaaaac ttggtgaaac aagaattgca gtgcattcgg gcgcaggaag
    10441 gattgtcgaa agacgtgggc gaaatcgtcg gcaagatttt gggttgaggc agtcaaacag
    10501 aaaaaaca
//
LOCUS       DAXDSD010000079        10350 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXDSD010000079 DAXDSD010000000
VERSION     DAXDSD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.079"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(500..1084)
                     /gene="rnhB"
                     /locus_tag="ACFB2Q_001605"
     CDS             complement(500..1084)
                     /gene="rnhB"
                     /locus_tag="ACFB2Q_001605"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC0918595.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1152..3047)
                     /gene="mnmG"
                     /locus_tag="ACFB2Q_001606"
     CDS             complement(1152..3047)
                     /gene="mnmG"
                     /locus_tag="ACFB2Q_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC0918596.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRSQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGAEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYAPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAIPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3220..4647)
                     /locus_tag="ACFB2Q_001607"
     CDS             complement(3220..4647)
                     /locus_tag="ACFB2Q_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC0918597.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            4806..5798
                     /gene="pdxA"
                     /locus_tag="ACFB2Q_001608"
     CDS             4806..5798
                     /gene="pdxA"
                     /locus_tag="ACFB2Q_001608"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC0918598.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(6028..8787)
                     /locus_tag="ACFB2Q_001609"
     CDS             complement(6028..8787)
                     /locus_tag="ACFB2Q_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC0918599.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     VPAAETSEKRSTANRSGSRANNRRRNPRRSKREGSKIEVREAAGKTAGQKARADKAET
                     RNNGNRRRNERGDRATERANEAEIQSRNVQPAAPVADAAPPETEGQTGKRRRNGSRNE
                     RGQTAPETAAVAETAVQTAENTPPEPYTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALIYDAAEKIRRAAADILPEGAAPKAAAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
     gene            9280..10272
                     /locus_tag="ACFB2Q_001610"
     CDS             9280..10272
                     /locus_tag="ACFB2Q_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC0918600.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAEPPPDLAQFAVMLENGTKM"
BASE COUNT         1985 a         2714 c         3208 g         2443 t
ORIGIN      
        1 cgtcattccc gcaaaaacaa aaaaatcaaa aacagaaaac tgaaatatcg tcattcccgc
       61 gcaggcggga atctaggtct gtcggtacgg aaacttatcg ggaaaaacgg tttttccaac
      121 cctgagactc cggattcctg ttttcgcggg aatccggttt tttgagtttc agtcattttt
      181 gataaattct tgcagctttg agtttctaga ttcccgcttt tgcgggaatg gcggtttgga
      241 agttacctga aattcaaaaa aaaaaacgga aaccggacgg attggattcc cgcctgcgcg
      301 ggaatgacgg attttaggtt tttttgattt tctgtttttc gcgggaatga cggttgggaa
      361 gtttcccgaa acttaaaaac aactgaaacc caaaaaacta aattaccgcc tgcgggggaa
      421 tgacggttcg ggttctttct ctttgaagtt gcgatgctgg aaatgccgtc tgaagttcag
      481 acggcatttt tgtgccggtt taaaacaagg cctgctgcgc gagcaggttt ctgacggggg
      541 cgaagtcgcg gcggtgttcg ggcagcacgc cgtattgttt gagggcttcc agatgctgct
      601 ttgtgccgta acctttgtgt ttgtcaaaac cgtattgggg acggcgttgc gccagtgcgt
      661 acatttccgc atcgcgtgcg gtctttgcca aaacggatgc ggcggagatt tcgatgattt
      721 tgctgtcgcc tttgacgact gcttcggcag ggatgcctaa atgttcggga atgcggttgc
      781 cgtcgatgaa tattttttcg ggacgcgcag ccaaaccgta aacggcacgt ttcatggcga
      841 gcatggtggc gtgcaggatg ttgaggcttg cgatttcttc gggcgtggag gcggcgacgt
      901 gccacgcgac cgcctgttct ttaatcattt cggcaagcgc gtcgcgtttt ttctcgctga
      961 gttttttgga gtcggtcagt ccgggcaggt cgaatgtttc cggaaggatg acggcggcgg
     1021 caaacacgct gccgaccaaa ggtccgcgtc ctgcctcgtc cacgccggcg gtcagtatgt
     1081 gcatgatgtt tcctgtcggg atggtacgat gccgtctgaa aatggtttca gacggcatcg
     1141 taccgatgtg tttatttcgc gtctttaaac ccgcgcttca aatgcaccat cagcagtgcg
     1201 acggcggcag gcgtaacgcc ggaaatgcgg cttgcctgcc cgacggtttc gggtttgtgc
     1261 tggttgagct tttgttgcac ttctgccgac aagcctttga ctctgccgta atcgatgccg
     1321 tcgggcagtt ttaaggtttc gatgtcgcgg cggctgtcga tttcttcgtt ttggcggtcg
     1381 atatagcctt ggtatttgac ttggatttcg acttgttcga ccacttcggc ggagaggcgt
     1441 tcagacggta tcgcgccttc gagcgtcatc agcgcggcgt aatcgaggtt cgggcggcgt
     1501 aacaaatcgt gcaggttggc ttcgcggctg agtttttgtc cgaacacgcg gatttgttcg
     1561 tcttcggcga gtttttgcgg cgcgtaccac gttgttttca aacgttggat ttcgcgttcg
     1621 acggcttcgc gtttttcgtt gaacatgcgc cattgcgctt cacccaccaa gccgattttg
     1681 tagccgtctt cggtcaggcg catatcggcg ttgtcttccc tgagttgcag gcggtattcg
     1741 gcgcggctgg taaacatccg gtaaggttcg ttcaggcctt tggtaatgag gtcgtccacc
     1801 aatacgccga ggtaggcttg ttcgcggcgc agcaggagcg gatcttgtcc gcggacgtat
     1861 tgcacggcgt tcgcgcccgc cagcaatcct tgcgcggcgg cttcttcata gcctgtcgtg
     1921 ccgttgattt gcccggcgaa aaacagtccc tcaatggttt tggtttcgag gcttgctttg
     1981 aggttgcgcg gatcgaagta gtcgtattcg atggcgtagc cggggcgcag gatatgggcg
     2041 ttttcaagac ccttcatact gcgcacgagc gcgatttgga tgtcgaacgg caggctggtg
     2101 gagataccgt tggggtagta ttcgtgcgtg gtcagacctt cgggttcgag gaaaatctgg
     2161 tggctgtctt tgtcggcgaa gcggttgatt ttgtcttcga tagacggaca ataacgcgga
     2221 cccacgcctt cgattttgcc ggtaaacatc gggctgcggt cgaagcctga gcggatgatg
     2281 tcgtgggttt gcgtgttggt atgcgtaatc cagcaggaca cttggcgcgg gtgcatttcg
     2341 gcgttgccgc gcacggacat gacgggaacg ggcgtgtcgc cgggctgttc ggtgagttgg
     2401 gaaaagtcaa tcgtgcgtcc gtcaatacgc ggcggcgtgc cggttttcag acggccttgc
     2461 ggcagcttca attcgcgcaa acgtccgccg agcgatttgg cggctgggtc gccggcgcgg
     2521 ccgccttcgt agttttccaa accgatgtgg attttgccgg acaagaacgt gcctgcggtc
     2581 agcaccacgg cgcgtgcttt aaactccgcg cccatcgcgg tgatcacgcc gctgatgcgt
     2641 tcgccctcga gtgttacgtc ttcgacggct tgttggaaaa ggtcgaggtt ttcttggttt
     2701 tccaacattt cgcggatgga ggctttatac aggatgcggt ctgcctgcga gcgcgtggct
     2761 cgtactgccg cgcctttgct ggcgttcagg cggcggaatt ggatacctga tttgtcggtt
     2821 gccaacgcca ttgcgccgcc gagcgcgtcg agttcgcgta ccaaatgccc tttgccgatg
     2881 ccgccgatag aggggttgca cgacatttgt ccgagcgttt cgatattgtg tgtgagcaaa
     2941 agcgtctgcg cgcccatacg ggcggcggca agcgcggctt ccgtgccggc gtgtccgccg
     3001 ccgacgacga tgacatcgta ggttttgggg taaatcatgt gggtcatgtg tgtattgcct
     3061 atcggttttt cagacggcat tccaagcagg atgccgtctg aaaaacgaaa cggattcaaa
     3121 agtaaagggt tgggattgta cgcctgttcg ccctgttttt acagtgcgcg gaaagggaaa
     3181 agccgcttcg cggggaagcg gctccggtaa gggtgggatt taccaaacat cggatttgat
     3241 gcggcgtttc aggcccggat gttcggaaag tttgaactcg gggtctttgc ccattttcag
     3301 cttggcggtg taatcgcgca gcagcataaa cgccaatggc gagagcagca ggatggcgac
     3361 gaggttgatc cacgccatga tgcccatcgc catatccgcc atatcccaga ccaaaggcac
     3421 attggcaacc gcgccgaaat agacccacgc caaaaccagc atacggaaaa cggcggtaat
     3481 cagccaatgg cttttgatga attggacgtt ggactcggca taggcatagt tgccgataac
     3541 ggtggaaaag gcaaacataa acaggatgac ggcgaggaaa cccgcgcccc attgccccac
     3601 ttggctgaca atcgccgcct gcgtcagcgc cgcaccgctc aaatcgccat aaggctgttg
     3661 gtaaatcaag atgatgaagg cggtgcaaga acaaacgatg atggtatcga caaacacgcc
     3721 cagcatttga atcatacctt gcgaaacagg gtgtttcact tcggcggcgg cggcggcgtt
     3781 cggcgcggaa cccatacccg cctcgttgga atacaggccg cgtttgatgc ccatcatcat
     3841 cgtttgcgaa atcagaccgc cgagtaagcc gcctgctgcc gcgtcgaatt tgaacgcgcc
     3901 cgaaaaaatc tgaccgaaca cgtccggaat catcggaata ttggtcaaaa tgatgaaaag
     3961 cgcgataaag aggtacaaaa ccgccatcag gggcacgacg atttccgccg ctttggatat
     4021 gcgcctgatg ccgccgaaga taatcggcgc ggttaaaatc accagggcga cgccgacata
     4081 atgaggctcc cagccccacg ccgctttgac ggtatcggcg atggtattgg tctgaaccgc
     4141 ttcaaacaca aaaccgaaac agaaaatcag gctcagggcg aacaacacgc ccagccattt
     4201 ctgccccaga ccgtgcgtga tgtagtaggc agggccgccc cggaaatggt ggttgtcgca
     4261 gtcgcggact ttaaagagct gcgccagcga agattcgaca aacgccgaac tcatgccgat
     4321 taaggcggtt acccacatcc aaaacaccgc gcccggcccg ccgactttga tggcgattgc
     4381 cacgcccgcg atattgccca cgcccacgcg gctggcaagg ccggttacaa atgcctgaaa
     4441 cggcgtgatg ccgtgagggt cgtccccctg tttgcggccg ccgagcattt ctttgatgct
     4501 gcgcccgaac aggcggaatt ggacaaagcc cgtggttacg gtgaagaaaa gccccgtacc
     4561 caaaagcata taaaccaagt atgaccacat cggatcgttg atggcgccga cccagccgtg
     4621 cagccattcg gtaaagttct cgttcatatc gcttccttaa agttgaaacc cgcacatatg
     4681 gcggtatgca agcagggttt aaattttgta aacgcccatt ctagcagatt gtcaacaaaa
     4741 tcagaaaaat ttacatcgcc gcgcgcctgt ggcgttagaa tcgcgttttg tttggagcaa
     4801 acacgatgaa acagcctgtt tttgccgtta cttccggcga gcctgccggc atcggccccg
     4861 atatttgttt ggacttggcg tttgcacgcc tgccctgccg ctgcgcggta ttgggcgaca
     4921 aacacctgtt gcgcgcgcgc gccgaagcct tgggcaaaag cgtcgtcctg cgcgacttcg
     4981 atccggaatc aggcggcgcg cacggagaag gcgggctgga agtgctgcac atccctgccg
     5041 ccgaagcggt tgaggcaggg tggctcaatc ccgccaacgc cgcctatgtg ctgcaacttt
     5101 tggacaccgc gctcgcaggc atttcagacg gcattttcga cggcatcgtt accgcgccgc
     5161 tgcacaaagg catcatcaac gccgcgcgcg caagcacagg ttttttcagc ggacacaccg
     5221 aatatctggc ggaaaaaagc ggcacggggc aggtcgtgat gatgcttgcc ggcaaaggcc
     5281 tgcgcgtcgc cctcgtaacg acccacctgc cgctgaaaga cgttgccgcc gccatcacgc
     5341 aaccgctgat tgaaagcgtc gcacgcattt tgcatcacga cttaaaacac aaattcggca
     5401 tcaaaaatcc caaaatccta gtcgccgggc tcaatcccca cgccggcgaa ggcggacacc
     5461 tcggacacga agaaaccgac accattatcc ccgcgctcga aaacctgcgc cgcgaaggga
     5521 taaacctcgc cggcccgtat ccggcggaca cattgttcca accgtttatg ctcgaaggtg
     5581 cggatgccgt attggcgatg taccacgacc aagggctgcc cgtgttgaaa taccacagct
     5641 tcgggcaggg cgtgaacatc acgctggggc tgccctttat ccgcacctcc gtcgatcacg
     5701 gcaccgcgct cgatttggcg gcaaccggca gggcggactc cggcagcctg ataactgccg
     5761 tggagaccgc cgtcgagatg gcgcgcggca gcctttaaag atgataaaag acccgtcatt
     5821 cccgcgcagg cgggaatccg gaccttcggg cggcggcaat attcaaaggt tatccgaaag
     5881 tttgaggttc tagattcccg ttttcacggg aatgacgaaa ggtggcggga atcacgggaa
     5941 tggcgaagtt tcaggcggta ttgcaggtat ccgaaaccat gcaaaaagag gttctgcgga
     6001 acagaacctc tttttgccgc cgtcggttca gccttgccgg gtttcgactt ggatcatttc
     6061 ttcggcaggg acggttgcgg cttcagacgg ttttggctgt tcggaacggc gcaaaccgcg
     6121 tccggcttgg acttcgggtt gtgccgccca tgccttcaat gcggcagggt cggtttcgat
     6181 caggacgagt ccgccggttt gtgcggtttc ccgtgcctgt tccgccgcag ccgtaaaggt
     6241 tgcggtttca gacggcattt cctgtgctgc ggctttcggt gccgcgcctt cgggcaggat
     6301 gtcggcggcg gcacggcgga ttttttccgc cgcatcataa atcagtgcgt cgccgtttga
     6361 aacggcggaa gatgccgtct gaacgggtgt ggcgatgagc ggatcggcaa tgctgacggt
     6421 aatcggcgcg tttgcgtcgg tttcgccgaa aacgtgcgcg gcggcggaac ggactttgtc
     6481 ggcggtgtcg tgaatattca ggtactgctc gattttggcg gcagacggga tattgcgttt
     6541 tttgccgttt tgacggcggt cgcgctgatt gttgcgttcg cggcgttctt tggcatcgcg
     6601 gctgtcgcgt tcgcggcggt tgcgttcgga tttgggcttg ctgcctttgt cttctgcggt
     6661 atacggttcg ggcggcgtgt tttccgctgt ctgaacggct gtttcggcaa cggcggcggt
     6721 ttccggtgcg gtttggccac gttcgttgcg gctgccgttg cggcggcgtt ttccggtttg
     6781 accttcggtt tcgggcggtg cggcatctgc aacgggtgcg gcaggctgta cgttgcggct
     6841 ttggatttcc gcttcgttgg cgcgttcggt ggcacggtcg ccgcgttcgt tgcggcggcg
     6901 gttgccgttg ttgcgcgttt cggctttgtc ggcgcgcgct ttctgtccgg cagttttgcc
     6961 cgccgcttcg cggacttcta ttttgctgcc ttcgcgtttg ctgcggcgcg ggtttcggcg
     7021 gcggttgttg gcgcggctgc cgctgcggtt tgccgtgctg cgtttttcgg aggtttcggc
     7081 agcgggtacg gcttgggttt cgctgccgcc gaaaatgcgt ttgagccatg ctttgaagct
     7141 gtcccaccag gaggtttttt tctcgggggc ggcagtcggg gcggggctgg tgtggcgcac
     7201 gcctttgacg gcgggttcgg ggcgggcggc tttggctttt tcgccgccga aaggtttggc
     7261 ggattcgtct tcttcaggct cggcaacgcg cttgtagctc ggttcgccgt cttcttctac
     7321 gtcgtcggtg cggatgcggt tgatttcgta gtgcggattt tcgaggtgga tgttcggaat
     7381 caggacgacg ttcacatcca aacgctcttc cattgcaaac agctcggcgc gtttttcgtt
     7441 cagcaggaag gtggcgacat cgacgggcac ttgggcgcgc acttcgccgg tgttgtcctt
     7501 catcgcttct tcctgaacca tgcgcaaaac gtgcagggcg gtggattcga tgccgcggat
     7561 cacgccggtg ccggcgcagc gcggacaggc ggcgtggctg ctttcgccca aagcgggttt
     7621 caggcgttgg cggctcaatt ccaaaagtcc gaaacgggag agtttgccca tctgtacgcg
     7681 ggcgcggtct tttttgagtg cgtcgcgcag gacgttttcc acatcgcgct ggtgtttggg
     7741 gttttccatg tcgatgaagt cgatgacgac caagccgccc aagtcgcgca ggcgcatttg
     7801 tcgggcgact tcttcggcag cttccatatt ggttttgaag gcggtgtctt cgatgtccgc
     7861 gccgcgtgtg gcgcgtgcgg agttcacgtc gatggagacg agggcttcgg tgtggtcgat
     7921 gacgatcgcg ccgccggagg gcaggctgac gctgcgcgag aacgcgcttt cgatctggtg
     7981 ttcgatttgg aagcgggaaa acagcggcgt gtggtcttcg tagagtttca gacgacctgc
     8041 gttgcccggc atgacgtagc tcatgaactc ggcaacttgg tcgtaaactt cttgattgtc
     8101 caccaaaatt tcgccgatgt cgggacggaa gtagtcgcgg atggcgcgga tgagcaacga
     8161 gctttccata aagagcaggt aggggtcgtg atgcgctttt cctgcttctt cgattgcctg
     8221 ccagagttgt ttgaggtagt tcaaatccca ttccaactct tccgcgctgc ggccgatgcc
     8281 ggcggtacgc gcgatgatgc tcatgccgtt cggaatgtcg agttgcgcca tcgcggcttt
     8341 gagttcttga cgctcttcgc cttcgatacg gcgggatacg ccgccgccgc gcggattgtt
     8401 cggcatcaat accagatagc gtccggcgag gctgatgaag gtggtcagcg ccgcgccttt
     8461 gttgccgcgc tcgtcttttt cgacttggac gatgacttcc atgccttctt tgagcacgtc
     8521 ctgaatgcgc gcgcgcccgc cttcgtagcc gaggaaatac gaacgcgata cttctttaaa
     8581 cggcaaaaag ccgtggcggt cggttccgta atcgacgaaa cacgcttcca gcgacggctc
     8641 gatgcgggta atgatgcctt tgtagatatt gcctttgcgc tgttctttgc ccagcgtttc
     8701 gatgtccaaa tccagcaggt tttgtccgtc gacgatggca acgcgcagct cttcggcctg
     8761 cgttgcgttg aataacattc ttttcatgat cacctcgtgg acaggcggca ttcgggcgcg
     8821 ccggtccggt tcggcattcc gtaaggctgg gtttccgatg tcttcggata aaaccggtaa
     8881 tcagtttttg agttgaaaat ccgcagggat gcacgttccg gggaaccgtg tgcggaaggg
     8941 tcggataaag aaggctataa agatcgatgc ggcggtctgc ctgccgcgtt ccgaacgctg
     9001 cggtcggaaa aatgggcgcc ggcttcttct tgttatcgtg atgcctgtgt tttcgggcgg
     9061 tttgcgttcg gggcttgggg ctccgctgcc gtcttacttc cgcgccgaaa cggcaaaatc
     9121 aattcaaact tgactacgtt ctgcgcctgc cggctgggaa caggcgcagg gaaaatgctt
     9181 tgcggagtgc gtttttaata taaaattccg ttttaaagta aaccgtttca gggggcgcgg
     9241 cggacgcgct ttctgctgaa acggatgttc ggattataga tgaaaacgca cgaaataagc
     9301 aaaggttcgg tcagcctgat aggggttgcc gaacatgagg cgggtcaacg ccttgataac
     9361 tatctgataa aaatcctcaa gggcgtgccc aagggctata tccaccgcat tatccgcgcc
     9421 ggcgaggtgc ggttgaacaa gaaacgctgc aaacccgaca gccgtattgc ggagggagat
     9481 acggtgcgga ttccgcctgt gcgcgtggcg gagaaggaaa tgccgtctga aaggcgtgcc
     9541 gccgtaccgg cgcgtgcgtt tgaggtggtg tatgaggacg acgcgctact ggtggtcaat
     9601 aagccttccg gtgtggcggt acacggtggc agcggcgtga gtttcggcgt tatcgaacag
     9661 ttgcgccgcg cccgtccgga ggcgaagtat ttggagttgg tccaccgttt ggacaaggat
     9721 acgagcggct tgttgatggt ggcgaagaaa cgcagcgcgc tcgtcaaact gcacgaagct
     9781 atccgtaacg accaccccaa aaaaatctac cttgcgctgg gggtgggcaa actgccggac
     9841 gacaatttcc atgtcaaact gcccctgttc aaatataccg gcgcacaagg cgaaaagatg
     9901 gtacgcgtca gcgaggacgg gcagtcggcg catacggtgt tccgtgtgtt aagccgtttc
     9961 tcagacggca ttttgcacgg tgtcgggctg tcgcacctga ctttggtgcg ggcaacgctg
    10021 aaaacgggcc gtacgcacca aatccgcgtg catctgcaat ctcaaggctg tccgattgcg
    10081 ggcgacgaac gctacggcga ttatcaggcg aaccggcgtt tgcagaaatt gggtttgaag
    10141 cgcatgtttt tgcacgcgtc cgagctgcac ctgaaccatc cgctcacggg tgagccgctg
    10201 gtgttgaagg cggagccgcc gccggatttg gcgcagtttg cggtgatgtt ggaaaacggg
    10261 acgaaaatgt gaaccccgat gccgtctgaa ggcttcagac ggcatcgggg cgtgaaagta
    10321 tagtggatta acaaaaatca ggacaaggcg
//
LOCUS       DAXDSD010000080         9766 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXDSD010000080 DAXDSD010000000
VERSION     DAXDSD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.080"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            491..2026
                     /locus_tag="ACFB2Q_001611"
     CDS             491..2026
                     /locus_tag="ACFB2Q_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC0918601.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTACVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            2023..2115
                     /locus_tag="ACFB2Q_001612"
     CDS             2023..2115
                     /locus_tag="ACFB2Q_001612"
                     /inference="COORDINATES: protein motif:HMM:NF028261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC0918602.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(2151..2591)
                     /locus_tag="ACFB2Q_001613"
     CDS             complement(2151..2591)
                     /locus_tag="ACFB2Q_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918603.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRGTGISSERKLWMRRARWNANGSRKTNAV"
     gene            complement(2785..4005)
                     /gene="lysA"
                     /locus_tag="ACFB2Q_001614"
     CDS             complement(2785..4005)
                     /gene="lysA"
                     /locus_tag="ACFB2Q_001614"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC0918604.1"
                     /translation="MTLFCEQVAYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(4016..4186)
                     /locus_tag="ACFB2Q_001615"
     CDS             complement(4016..4186)
                     /locus_tag="ACFB2Q_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC0918605.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGAIQTGLQL
                     QGKPQSAPPTQK"
     gene            4257..4580
                     /gene="cyaY"
                     /locus_tag="ACFB2Q_001616"
     CDS             4257..4580
                     /gene="cyaY"
                     /locus_tag="ACFB2Q_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC0918606.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            4613..5032
                     /locus_tag="ACFB2Q_001617"
     CDS             4613..5032
                     /locus_tag="ACFB2Q_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC0918607.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            5054..5560
                     /gene="luxS"
                     /locus_tag="ACFB2Q_001618"
     CDS             5054..5560
                     /gene="luxS"
                     /locus_tag="ACFB2Q_001618"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC0918608.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            5704..8496
                     /gene="polA"
                     /locus_tag="ACFB2Q_001619"
     CDS             5704..8496
                     /gene="polA"
                     /locus_tag="ACFB2Q_001619"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC0918609.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            9058..9753
                     /locus_tag="ACFB2Q_001620"
     CDS             9058..9753
                     /locus_tag="ACFB2Q_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC0918610.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
BASE COUNT         2276 a         2551 c         2640 g         2299 t
ORIGIN      
        1 ggccgccttg tcctgatttt tgttaatccg ctatatttcc cgccatccca aaaacgaaga
       61 gcggcaggaa tttatcggaa aaacagcaac ctttccgccg tcattcccgc gaaagcggga
      121 atctaggttc gtccggtttc ggttatttcc gatagattcc tgccgcgttg ggggtctgga
      181 ttcccgcctg cgcgggaatg acgggacttt aggtttctgt ttttgttttt ctgtttttgc
      241 gggaatgacg gggaagttgg cggttgtttc ttatggagtg gtttttgtat ttggataaaa
      301 aatttttcgt tttcaagcct tcaccgcttg ccatcggcgt taaaattttt tacgataagc
      361 acatagattg taaacaatcg gccacaagcc ggtttgtttt ttcagaagac attatccctg
      421 tcagacgctg tttctatata tgtttgccta taacggcttg tttttaataa ataattcaag
      481 aggtatcaac gtgtctgatt ccaaaacgaa agaacgcgcc acattcggca cgcgccgcgc
      541 gtttatgatt gccgccatcg ggtccgccgt cggcttgggc aatatttggc gtttccccta
      601 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      661 ggcgggcatc ccgctgctgc tgctcgatta tgccatcggc caccgttacc ggggttctgc
      721 gcccttggct ttccgccgcc tcggacgctg gttcgagccg gtcggctggt ggaacgtgat
      781 gaccaatatc gtcatctgca tctattacgc agtgattatc ggttgggcgg caagctatac
      841 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaggactt
      901 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tcgccggccc
      961 tttggcgggc gtgtgggttt ttaccgccgc cattatggct ttgggcgtgc aaaagggcgt
     1021 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
     1081 catttcgctg acgctgccgg gtgcggcaaa gggcttggac gcattgttta cgcccgactg
     1141 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
     1201 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
     1261 gggcggaacg gggctggtgg tcggttttgc caacagcagc ttcgaactgc tcgcgggcat
     1321 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggcaaggcgg tcaacgaggt
     1381 tgcctccggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcgcc
     1441 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtgttcgccg gcgttacgtc
     1501 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1561 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1621 tacggcgacg gggctgcctg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1681 tgttgccgcc ggctttgttt atgttgccgc catcatcatc ggcggcaggt tgccggaatt
     1741 acgcaggcac ctgaacgcct tgtcctccat ccgcgtcggc ggcttgtgga cggcctgcgt
     1801 cgtgtttacc gtcgtgatgc tcggctatat gctgtatcag gacaccgccg gattgttgga
     1861 aaaaaactac ggcgattatc cggatggttt cctcaatatt ttcggctggg ggatgtcggc
     1921 ggcgttaatc atgttcgggc tgctgctgtc gctgctgcct tggaaacacg gtcaggattt
     1981 taatgtcaaa gacgaacacg aacatgaaca aggaggagaa aaatgagtac ttccgccatt
     2041 gtgatgatgg ttgccgccat cgcggtgatt tggggcgggc tgctgctttc cctgttaagg
     2101 ctgccggaag agtaagcctt tagaacgtta aaaatgccgt ctgaaccgct tcagacggca
     2161 tttgttttac ggctgccatt cgcgttccac cttgcccgac gcatccaaag tttgcgctcg
     2221 gacgatatgc ccgtcccccg gtacaggatg cgcgtccgca atatgccctg ttcggtatag
     2281 ccttgcgcca cgccgttttc aaccggcgtg tgcgaatgca gtttgccgtt tggatggtaa
     2341 atatcgacaa agcggtcgaa cacgccgttt ttgacgatgc ttttcaagac gcgcccgttt
     2401 gaggcgcggg tgctgaatgc gccgtccggc gtgcctgaag gcggccggct gtatgggttg
     2461 gcgggttgga tgggcgcgct tttcggcagg gtgcgaagcg gcggccggtc agagggaagg
     2521 gcggtgcagc ccgccgaaag tagggcggcg gacgaagcgg taagcaggag gataatcgga
     2581 gcgtgcttca tttcaattcc ttagatattg caccccataa aaggggcgcg taattttttt
     2641 caacaattcc cttcgtcagt cgggcgagac ggggatttgt tgcgtcgtca tttgaaaaca
     2701 ggctgtgttt gaatattccg ccaagatgtc actttaaacg gacaatgccg tctgaaggct
     2761 tcagacggca ttttgatgtt cggcttatag gcaggcgagt tcgtttgcca tttgctgttc
     2821 caaggtttcg cgccggcgga tgagtttgta tccgccgccg tcaaccaaca cctccgccgc
     2881 acggttgcgc gtgttgtaat tgctagccat actggccccg tatgcgcccg cgctgcggat
     2941 aagcagcaaa tcgccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt
     3001 ttcacaaatc gggccgacga tgttggcggt cagaggctca atgtttttgg tttcaaccgc
     3061 ttcgatgtgg tggtaggcat cgtatagggc tgggcgcatc aaatcgttca tcgccgcatc
     3121 gaccatcaca aagtttttct cttcaccgtg tttgacaaat tcgacgcgcg tcagcaatgc
     3181 acctgcgttg ccgaccaagc tgcggcctgg ctcaagaatg agtttcagac ggcgtgtccc
     3241 catcagtttt tgaaccgctc gggcatacgc acccaaatcg gggacgcctt cgtctttgta
     3301 aacaatgccg acgccgccgc ctaagtccaa atgttccaaa acaatgcctt cggcggcaag
     3361 agcgtcaacc aaaatcaaaa tgcgttcgca ggcttcgacc agtgggctta agtcggtcag
     3421 ttgcgaaccg atgtggcagt cgatgccgat gattttcaaa ttgggctgtt gtgcggcatg
     3481 gcggtaggct tcgagcgcgt cggcgtaggc gatgccgaat ttgttggctt tcagacctgt
     3541 ggagatgtag ggatgggttt ttgcatcgac atcgggattg acgcgcaggg agacgggcgc
     3601 ggttttgccc aaacgcgcgg caattttctg aatgcggtcg atttcgggga tgctttccat
     3661 attgaagcat tttacgccgg cattcagcgc gaactcgatt tccgcctcgc ttttgcctac
     3721 gccggaaaaa atcgttttcg ccgcatcgcc gcctgccgcc aaaacgcgtg ccaattcgcc
     3781 gcccgacaca atgtcaaaac cgctgcccaa agaagcaaag tgtttgataa tgctcaggtt
     3841 gccgtttgcc ttgacggcgt agcagacaag cgggttcaaa gcggcaaagg cggtttgata
     3901 gttttcaaat gctccggtca gcgcggattg gctgtacaca taaagcggcg tgccgaattc
     3961 ttcggcaagg cgggggtagg cgacttgttc gcaaaacagg gtcatatttt cgttttcatt
     4021 tttgggttgg tggggcggat tgcggtttgc cttgaagctg caaaccggtt tggattgcgc
     4081 cgaaacgcgc cgtatcgcct tctttgggca ggtagaggcc gcctttgtaa ccgcaggcgg
     4141 aaagcaggag ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt
     4201 cataagcgcg aatgtggcaa gattcggcat cttaaacaaa aaacacgcaa aaagctatga
     4261 tgaccgaaag cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa
     4321 tcgacgaaaa cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag
     4381 ccggagacgg cacgcaaatc atcgtcaacc gccacacgcc caaccaagaa ttgtggattg
     4441 ccgcaaaaag cggcggctac catttcgccg aacaaaacgg caaatggctg gcaacgcgcg
     4501 acagccgcga tttttacgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg
     4561 ttgagattgc cgaattgtga ttcaattttc gatataaaaa gattgttacc caatggcaca
     4621 attacctcta tatcggactg ccgaaatcgg aaattttact gtcggcacgc ctaaagtttt
     4681 agaatcattt tccaaacata tcccttatgg tgttgtcttt gaagatgatg gcaacacggg
     4741 ctacttctat gccgcttcgc aagaggggat tttggatgcc ttgcacatct acaatgtcga
     4801 agatgtatcc gacaaacata tccccaatca tgtcttgatt ttatgggatg gcgcctgcac
     4861 catagccgca ttgtgtatca acgactacat ccatgccgtc tatgattttg tcgaacaggc
     4921 aggatattgc cgcaacggct tccctgaagc aggcggcgaa tgggtaaaag tcgaaaaccg
     4981 cgtcttggac gatgaattgc tggacaaaat cctatcccga aaacctacat aacccgcaca
     5041 aaaggatacc caaatgcccc tgttagacag tttcaaagtc gatcacaccc gtatgcacgc
     5101 ccctgccgta cgcgtggcga aaaccatgac cacgcccaaa ggcgacacca ttactgtatt
     5161 cgacctgcgc ttttgcatcc ccaataaaga aatcctgccc gaaaaaggca tccacacgct
     5221 ggagcatttg tttgccggct ttatgcgcga ccacttgaac ggcgccggcg tggaaatcat
     5281 cgacatttcc ccgatgggct gccgcaccgg tttttatatg agccttatcg gcacgccttc
     5341 cgaacagcag gttgccgatg cgtggttggc ttcgatgcag gatgttttga atgtcaaaga
     5401 ccaaagtaaa atccccgagt tgaacgaata ccaatgcggc acttatctga tgcactcgct
     5461 cgccgaagcg caacaaatcg cgcaaaacgt gttggcgcgc aaagtggcgg ttaaccgaaa
     5521 cggcgatttg gctttggatg aaagcctgct gaacgcttaa tccgccgcaa atgctgtctg
     5581 aacaagggtt tcagacggca tttgcctttt ccgttataat ccggggttgt acgggggcgg
     5641 gttttaagcc ggcatcgtcc ttccctattt ttctgtccct tatcggtttc aagcgggttt
     5701 tttatgtcca acagacctac actcctcctc gttgacggct cttcctatct ctaccgcgcg
     5761 tatcacgcga tggggcaaaa cctgactgcg cctgacggtg cgccgacggg tgcgctgtat
     5821 ggcgtattga atatgttgcg ccgtttgcgg tcggaatatc cgcacgatta ttgcgcggtg
     5881 gtttttgatg cgaaaggcaa aaatttccgc catcaaatgt ttgaagaata caaggcgacg
     5941 cgcccgccga tgccggacga tttgcgcccg caggcggaag ccctgccgga tttggtgcgc
     6001 ctgacgggct ggccggtatt ggtgattggg caagtggagg cggacgatgt gatcggcacg
     6061 ctggcgaaac agggggcgga acatggtttg cgagtcattg tttcgacggg cgataaggat
     6121 atggcgcagt tggtggatga gcgcgttacg ctggtgaaca cgatgagcgg agaaacgctg
     6181 gatattgaag gcgtgaaggc aaaattcggc gttcgccccg accaaatccg cgattatctc
     6241 gcgctgatcg gcgacaaggt ggacaacgta ccgggcgtgg aaaaatgcgg cccgaaaacg
     6301 gcggtgaaat ggctggaagc ctacggttcg ttgcaaggcg tgatggaaca cgctccggaa
     6361 atcaaaggca aggtcggcga aaacctgcaa gccgcgctgc cccaactgcc gctgtcgtat
     6421 gatttggtca cgattaaaac cgatgtggac ttgcacaccg agctttcaga cggcatcgaa
     6481 agcctgcgcc gtaccgcgcc gaaatgggcg cagcttgcgg ttgatttcaa acgctggggc
     6541 ttccgtactt ggctgaaaga agcggaatca aacatgaata ccggctcgac cgatgatttg
     6601 ttcggtagcg acagcatcgg cgagcaggcg gctttgaatg cggaaatacc gtctgaaaaa
     6661 cgagctgaaa aagccaccgc ccctgaaaaa ctggattatc aagccgttac caccgaagct
     6721 cagtttgccg ccttgttgga caaactagcg aaggcggaca caatcggcat tgatacggaa
     6781 accacgtcat tagatgcgat gaatgccgag ctggtcggca tcagcattgc gttccaagca
     6841 ggcgaagcgg tttacatccc cgtaggccac agcctgactg ccgcgcccga acagcttgat
     6901 ttgcaagacg tattaggccg tctgaagccg catttggaaa accccgccct gaaaaaaatc
     6961 ggtcaaaacc tcaaatacga ccaacacgtt ttcgccaact acggcatcgc gttaaacaac
     7021 attgccggcg acgcgatgct cgcttcctac atcatcgaga gccatctcgg acacggcttg
     7081 gacgaattgt ccggacgctg gctcggttta gaaaccatta cctacgaatc gctgtgcggc
     7141 aaaggcgcga agcaaatcag ttttgccgat gtcgccatcg ggcaggcgac cgaatacgcc
     7201 gcccaagatg ccgatttcgc cctgcgcctc gaagcgcgcc tgcgcgcgca aatggacgac
     7261 aaacaacttg aaatgtatga aaaaatggag cttcccgtcg cgcaggtatt gtttgaaatg
     7321 gaatgcaacg gcgtgcaaat cgaccgcgcc gaactcgccc gccaaagcgc ggaactcggc
     7381 gcggagctga tgaagctcga acaggaagcc tacgccgctg caggtcaacc gtttaacctc
     7441 aactcgccca aacagctgca agaaatcctg ttcgacaaaa tgggcatccc gacaaaaggg
     7501 ctgaaaaaaa ccgccaaagg cggcatttcc accaacgaag ccgtgctcga acagctcgcg
     7561 cccgactacc ccctgcccaa aatcatcctg caaaaccgca gcctggcgaa actcaaatcc
     7621 acctacaccg acaaactgcc cgaaatgatt tcgccccggg ataaccgcgt gcataccacc
     7681 tatgcccaag ccgtcgccat taccggccgc ctcgccagca acaaccccaa cctgcaaaac
     7741 atccccattc gcaccgccga aggccgccgc gtgcgccgcg ccttcactgc accgccgggc
     7801 agcgtcatcg tttccgccga ctattcccaa atcgagctgc gcattatggc gcacctctcc
     7861 ggtgacaaaa ccctcattgc cgcatttcaa agcggcgaag acgtacaccg ccgcaccgcc
     7921 gccgaagtgt tcggcactgc gcccgaaaac gtctcgcccg agcaacgccg ctacgccaaa
     7981 accatcaact tcggcttaat ctacggtatg gggcaatacg gtttggcaaa atcattgggc
     8041 atcgacaacc tttccgccaa aaactttatc gaccgctact tcgcccgtta tcccggcgtc
     8101 gccgaataca tgcagcgcac caaagaacaa gccgccgccc aaggctacgt cgaaaccctg
     8161 ttcggcagaa ggctctattt gcccgacatc cgcaacaaaa acgccaacgc ccgcgcagga
     8221 gccgagcgtg ccgccatcaa cgcccccatg caaggcaccg cttccgacct catcaaacgc
     8281 gccatgataa acgtgcgaaa ctggctttca gacggcatcg gcagcaaact ggtcatgcag
     8341 gtgcatgacg aactggtgct ggaagtcgtt gaaaccgaac tggattttgt caaagaaaaa
     8401 ctgccgcaga ttatggcgaa agtggacggc ggattattgg atgtaccgct ggtggctgag
     8461 gttggcgtag gggagaattg ggaagaggca cattgagaat gcacaagtat tgaactgtta
     8521 cgttttgatt tatcaaaatt taaggaagta aaatacccga ttcgaatgat tggaaatcaa
     8581 tagcaagccg taggtcggat acttgtatcc gacaaaagcc tgccatctca aatagcagtc
     8641 ggattcgaga atccgacctg ccaaaccggg cgcggacgct ccggtcggca gttagcgcac
     8701 aaatcgaaca gaacatcaca aaaaagcctg attcggattt tccaatcggg cttttttgcg
     8761 cccgttttgt catcccgtga aatatccgca taacaaaaat aatgcaaaat catgacaaaa
     8821 ccggcgcgag gttacacaaa cggatgaaat caaccgatat tcaaacacag ccatttttag
     8881 cgcattttca gcgtatcttt atgcggaaaa tttcgtgaac aggttttttg cacaggcttc
     8941 cgccgcgttt cgcgggatgg gaaaccgtat tagaaaacgg acgcacgcaa cataaacccc
     9001 gaaagtgatg ataagatgat gatttaacgt actgctttaa ttatttaagg aattatcgtg
     9061 tttcccgaca aatacaagtt gagtttggaa gaaaatattt ttctggcaaa gaaagtattg
     9121 gttgctcaaa ttcacaacct cagccgtttt gagaattgtc agacgacctt gttgcagacc
     9181 gagcaaatta tcaatggcaa aaatgtagcc tccgcgtcac tggaagacat ccaaaccatc
     9241 ttgaacctga aacgtgccta tcaatatgtg atttcgcata tttcaaacgg cgaaccggtc
     9301 aatatttcac tccttaaaag aatcaacaac attgttgcca aggacgattc tttggtaccc
     9361 gtggatttcc gtaccggttc ggtcggcgta acgctattgg acggttcccg tcatgccccg
     9421 aatccggtga aggaaattga agtggcccgc gtgttgcaaa atatcggact gcaaagcggt
     9481 tcgacgacgg aagcagccgt tcgtttcatg ctctattgta tgcggcagca ggttttttgg
     9541 gacggcaaca aacggacggc aaccctattt gccaacggcc tgatgatggc ggggggctgc
     9601 ggtatcctgg aaatctccga aatgcagatg ccgcgattca atgaaaaact atccgcattc
     9661 tatcgcaccg gcgacgatac cgatatttcc aagtttgtgt atcaaaattg tatatcgggc
     9721 atagactatt tcggcgcgga cgaagatata tagtggatta aattta
//
LOCUS       DAXDSD010000081         9288 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXDSD010000081 DAXDSD010000000
VERSION     DAXDSD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.081"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            289..1143
                     /locus_tag="ACFB2Q_001621"
     CDS             289..1143
                     /locus_tag="ACFB2Q_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin-like passenger domain-containing
                     protein"
                     /protein_id="HFC0918611.1"
                     /translation="MSNGARWTVTNDSMLKELDLSEDAQVEFSDNNKFVKVSVSKLKG
                     DGGVFKMYGDIVKGESDKLITRKGSEGTHIIEYMDDAKAKTTGREYLKLVENKGNQED
                     NKASNKASYKLNVRCTEQGGWCFALGESGASKKVNISTDGKRDFYLYPDTLTPGASSS
                     VLFGEALYQLNAVSDETLVQRMGEIHADGTPQEDNNVWIKRVGGKFSGSRSDYRVGGY
                     GNRYWGFAGGFNRTGFGDKWIHYKGLMLRHLQSSYASEDYVGSGKILRQGGRCLFRLA
                     QPGKQGLL"
     gene            1233..1691
                     /locus_tag="ACFB2Q_001622"
     CDS             1233..1691
                     /locus_tag="ACFB2Q_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC0918612.1"
                     /translation="MPICFPPKPVGGWKNKTAAKTYRWQPEVQLSYWFTRGYGFPLSN
                     GLSAETDNFRSLMGRFGFRAGVDGLDGGRLNIYGKLMYKREFIGTIRHRFNGSAVEEF
                     KHRGGWLEYGLGVVRRNAGNGRQLYFEAQRSSMHTMRQNWQVNMGVRSMF"
     gene            complement(2174..3586)
                     /gene="trkA"
                     /locus_tag="ACFB2Q_001623"
     CDS             complement(2174..3586)
                     /gene="trkA"
                     /locus_tag="ACFB2Q_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC0918613.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3684..5207)
                     /locus_tag="ACFB2Q_001624"
                     /pseudo
     CDS             complement(3684..5207)
                     /locus_tag="ACFB2Q_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(5514..6320)
                     /locus_tag="ACFB2Q_001625"
     CDS             complement(5514..6320)
                     /locus_tag="ACFB2Q_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC0918614.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6372..6623)
                     /locus_tag="ACFB2Q_001626"
     CDS             complement(6372..6623)
                     /locus_tag="ACFB2Q_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918615.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6725..7495
                     /locus_tag="ACFB2Q_001627"
     CDS             6725..7495
                     /locus_tag="ACFB2Q_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918616.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7641..8810
                     /gene="metZ"
                     /locus_tag="ACFB2Q_001628"
     CDS             7641..8810
                     /gene="metZ"
                     /locus_tag="ACFB2Q_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC0918617.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            8905..9111
                     /locus_tag="ACFB2Q_001629"
     CDS             8905..9111
                     /locus_tag="ACFB2Q_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC0918618.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         2126 a         2280 c         2712 g         2170 t
ORIGIN      
        1 ttaagcagaa acgattgtct caaaaaaagc aactttggat aaatacaatc aagaattggc
       61 tgaactaggt gaaaatgaaa agaaaaaaga agaactagaa aaaaagtaaa acagcaagaa
      121 aacaaaattc agatcagaaa aaggcacata gaacgtctaa aagaagacat agaaaaggtt
      181 gcggtactcg aagaagaaat caaaagatct gaaaagttct tcgatgaaaa cggttggctt
      241 aaagacagca ttatcgacga caaaacaaaa aacgccgtca acctcaaaat gagcaacggc
      301 gcgcgttgga cggttaccaa tgattcaatg ctgaaagagt tggatttgtc ggaagacgcg
      361 caggtcgaat ttagcgacaa caataaattc gtcaaagtgt ccgtgagcaa gctcaagggc
      421 gatggcggcg tgttcaaaat gtatggcgac atcgtcaaag gagaatcgga caagctgatt
      481 acccgaaaag gcagcgaagg gacgcacatc atcgaatata tggacgatgc caaggcaaaa
      541 acgacgggaa gggaatatct gaaactggtc gaaaacaagg gcaatcaaga agacaataaa
      601 gcatcaaata aagcatcata caaattgaat gtccgctgta cggaacaggg agggtggtgt
      661 tttgccttgg gtgagtcggg tgcttccaaa aaggtcaata tttccacaga cggtaagcgg
      721 gacttctacc tctacccgga taccctgacg ccgggcgcat cgagcagcgt ccttttcggc
      781 gaggcgttgt atcagttgaa cgcggtttcc gatgaaacgc tggtgcagcg tatgggcgaa
      841 atccacgccg acggaacgcc gcaggaagac aacaacgttt ggatcaaacg cgtcggcgga
      901 aaattctccg gcagccgcag cgattaccgc gtgggcggtt acggcaaccg ctattggggc
      961 tttgccggcg gcttcaacag gacggggttc ggcgataaat ggattcatta caaagggctg
     1021 atgctccgcc acctccaatc gtcctacgct tccgaagact acgtcggcag cggcaaaatc
     1081 ttacggcagg gcggcaggtg tttattccgc ctggctcaac cgggaaagca gggcttatta
     1141 tgatttggtc gccaacatcg cccgatataa gggcagctac gggctgacca attacgccgg
     1201 caaacgggtc gaatcggacg aggcgcgcct gaatgcctat atgctttccg ccgaagccgg
     1261 taggcggatg gaaaaacaag acggcggcaa aaacctatcg gtggcagccg gaagtgcagt
     1321 tgtcttactg gtttacgcgc ggctacggct ttccgctgtc aaacgggctg tctgccgaga
     1381 cggacaactt ccgcagcctg atggggcggt tcggctttcg ggcgggcgtg gacggtttgg
     1441 acggcggcag gctgaacatc tacggcaagc tgatgtacaa acgcgagttt atcggcacga
     1501 tccgccacag gttcaacggc tctgccgtgg aggaattcaa acaccggggc ggctggttgg
     1561 aatacggctt gggcgtggtg cgccgcaatg ccggaaacgg acggcagctt tattttgagg
     1621 cgcaaaggtc ttcgatgcac acgatgcgcc agaattggca ggtcaatatg ggcgtgcgca
     1681 gtatgttctg atgcgcggat acgcctgaaa acaatccgga acggtcagac gcgttccgga
     1741 ttgttttttt gtcggggcgg tttgctgcca tatcaaaaca ggcgggcgat gccgtctgaa
     1801 acttcgtcat tcccgcgaaa gcgggaatct agaacctcaa actttcagat aatctttgaa
     1861 tattgctgtt gctcaatggt ttggattccc gcaacctttc gtcattcccg tgaaaacggg
     1921 aatctagaat ctctaaagct tcagctaacc tttgaatatt gccgctttcc aatggtctag
     1981 attcccgcct gcacgggaat gacgatttgg aaattacccg aaacccaaaa acaactgaaa
     2041 ccgaacaggc cggattcccg ctacgcggga atgacggggg gcggatgccg tctgaagcgg
     2101 tgggctgaag cccactctac tattattttt gcttccatac cgcttggcgg caatgtgtcc
     2161 ggaatggagc ggtttatccg aaaaagccca ttttgacttg gatgagtttc tccagttcgt
     2221 tcaggatgcg ccggcgcgag acgaaaaaga tgatgtggtc accgtcttgg atgacggttt
     2281 cggtatggtg tcccataatg gtttcgccgg ttccggcgcg gacgacggcg gcaatgtggc
     2341 agccttcggg ccatttgatg ccgctgatgc gcctgccgat gatggcggaa gtttttttgt
     2401 cgccgtgcgc gacgacttcg atggcttccg ccgtgccgcg ccggatgggg tggacggcaa
     2461 cgatgtcgcc gcgccggatg tgggcgagta tcgagccgat ggtgatgagg tggggggaga
     2521 cgacgatgtc gattttgttg ccttcgagca aatcgacgta gcttgagcgg ttgacgatgc
     2581 cgatgacgcg cttcgcgccg aggtttttcg ccaaaagggc ggacataatg ttgctttcgt
     2641 cgtcgttggt cagggcgcag aatacgtcga tttcgtcgat gtattcgttg tcgagcaggg
     2701 tttcgtcggt tgccgaacct tgcaggacga gggtgttgtc gaggttttcg gctatccatt
     2761 cggcacggcg cggccggcat tcgatgattt tgacgttgta tgcgtgttcg agctgcttgg
     2821 cgaggcggta gccgatgttg ccgccgccgg caatcatgat gcggcgggtg ctggtttctt
     2881 tggggcgcaa ttcgggtatg accgccccga tgttttcggc ggcggcggca aacaggattt
     2941 cgtcgccttc gatgatgacg gtttgcggcg cggggacgat gaggcggttg ttgcggtaaa
     3001 cggcgcagat ttggcagtcg gccccgtcgg gcaaatcttg ggcgatgtcg gcaatgctgc
     3061 gtccgacaag cagcccgccg cgccgcgcct gtatgatgac catccgcacg cggtcgtctg
     3121 caaaacgtaa aacctgcaat gcgcccgggc agtctatcag gccggcaagc tgttcggtaa
     3181 cgagctgttc ggggctgatg gtttcggtta tgccgaatat ggaaaggctg ccgttttcgt
     3241 tgttttcgag cttggggctg aggtattcga ggtattcgct ggaacggacg cgcgcgatgc
     3301 ggccggggat gttgaacagg tcggcggcaa ctttgcaggc gacgatgttg gtttcgtcgc
     3361 tgcgggagag cgcgagcagc aagtcggcat cttccgcgcc ggcgcgttct aatgtgaagg
     3421 gggatgcgcc gttgccgaaa acggtttgga catcgaggcg gctgcctgtt tcctgcaatg
     3481 ctttttcgtc gatgtcgata acggttacgt cgttgttggt gatggcggca aggttttgcg
     3541 cgacggtaga acctacctgc ccgttgccta aaatgaggat tttcacggta tgggtcgccg
     3601 ggtgatgaaa tggtggattg taccgcaacc ggcggcggaa atgccgtctg aaggcagatt
     3661 ttgcgttcag acggcattgc gcttcaagac tcgacgggga tgatgccgat tttcgcctgc
     3721 catttgcgcg gggcggtggc gtggatggat tcgcctttgc tgtccacggc gacggttacg
     3781 ggcatatctt tgacttcaaa ttcgtaaacg gcttccatac ccaattcggg gaacgccaag
     3841 actttggaag atttgatggc ttttgccacg aggtatgccg cgccgccgac tgccatgagg
     3901 tacacggcct tgttgtcggc gatggcttcg caggtggccg cgccgcgctc ggatttgccg
     3961 atcatgccca agaggccggt ttgtttgagc atttggcggg taaatttgtc catgcgggtg
     4021 gctgtggtcg gacctgcggg accgacgact tcatcgccga ccggatcgac ggggccgacg
     4081 tagtaaatca ggcggttggt gaaatcgacg ggcaactcct cgcctttgtc gagcatattg
     4141 acgaggcgtt tgtgcgcggc atcgcggccg gtgaggattt tgccgttcaa cagcaatacg
     4201 tcgccggttt tccagcttgc cacttcttct ttggtcagct tatcgacatc gacgcgtttg
     4261 ccgttgtcgg ggctgtaagt cagatcgggt cagtcttcga cgcgcggcgg cgtgagttcg
     4321 acaggacctg agccgtccaa ttcaaattcg acgtggcggg tggcggcaca gttgggaatc
     4381 atggcaatcg gtttggaggc ggcatgggtc gggtaatcga ggattttcac gtccaacacg
     4441 gtggtcagac cgcccaagcc ttgcgcgccg atgcccagcg cgttgacctt ttcaaagagt
     4501 tcgaggcgca gggcttcggt ggtggacaat tccgcgccgg acgcggcttt ttcctgcaat
     4561 tcttggatgt cgatgtggct catcagggat tctttcgcca tcaacacggc tttttcgggc
     4621 gtgccgccga tgccgatgcc caagatgccg ggaggacacc agcccgcgcc catcgtcggg
     4681 atggttttca atacccaatc gacgatgttg tcggaagggt tgagcatagc gagtttggat
     4741 ttgttttcag agccgccgcc ttttgccgcg caggttactt cgactttacc gcccggcacg
     4801 atgctcatgt ggatgacggc gggggtgttg tctttggtgt tttggcgttt gccggccgga
     4861 tcggcgagga cggaagcgcg cagggtgttg ccttcccaag tgtaggcgcg gcgtacgcct
     4921 tcgttaacca tctcttccac gctcatgtcc gcatcccatt gcacatccat accgactttg
     4981 aggaagacgg ttgcgatacc tgtgtcttgg cagatggggc ggttgttttc ggcacacata
     5041 cggctgttga ccaaaatctg cgtcatcgcg tctttggcgg cgggattttc ttccttctgc
     5101 cacgccttat aaagcgcgtc gatgtagtct tttggatggt agtagctgat gaattggaag
     5161 gcatcgcaga tactttgaat aaagtcttct tgcttgataa cggtcatgat tgtgttcctt
     5221 ttcaaggttg tggcggtaag tgtctgctgc tgttttaaac aacctttttg aaggctgttt
     5281 tttatcaggt tgaaatagtg ccagcgtttg tacggtgaag acaatacata attctaatct
     5341 cttgattatc aatttgtttt aataaaatac tgttttattc ggattttgat atctgtaatc
     5401 tttgcctgtt gctttgcggt agggagaaat cagcaggcag ttcattgtgg accggttgat
     5461 gaacaatgcc gtctgaaagg gcttcagacg gcattttgtt cggacgggcg ggcttatttc
     5521 gcagcctgtc cgccctcttt ggcaaaatat ttggcgtaga tcttgtcgta ttcgccgctt
     5581 tcgcgtactt tttccaacgc atcgttcagc attttgacgg ttgcttcgtc gcctttgcgt
     5641 accgcgatgc cgtagtgttc ggtggtgaag tcgggcaggg taacgaagtc cattcctttg
     5701 gccgggttgt ttttcacata attggcgatg accgcgctgt cgctgaccac ggaatccaag
     5761 ccgccgtttt ccagttcttt gataatcagg gggacgtttt cgaagcgcgc gattttcgga
     5821 ttgtcgttgc ccaagagttt ggaaacggag aaatcgcccg tgtggccggt aaccacgccg
     5881 actttgttca tctttttcaa atcttcggaa gaagatactt ttttgccttt cggaacgagg
     5941 acgacttggg tgatttcaaa atacgggtcg ctgaaatcca tagactgttt gcggtcgtcg
     6001 gtaatggtta cgcccgacat cacaacgtcc gcatcgccgt tgttcaaggc ggggaaaagg
     6061 ctgtcccacg gctggtgttt gaattcgatt ttaaaattgc ccgccttcgc catcgcgttc
     6121 atcaaatcca cgtcgaaacc ttcgacattg cctttcgagt ctaaagattc aaagggggca
     6181 aactcggcgt tggaagccac gcggtacact ttgccggggt tggcggcagg cgcggcggca
     6241 tctttgccct gaccgccgca ggcagacagc gcgagtgcgg aacaggcaag ggcggcggca
     6301 atccattttt tcatattcat caaaattcct ttcggtaatc aaaaagccta aaaaatcagt
     6361 ttaacataaa attaacgttc cttttcacgg aaaaacgcat aagtaacgtc gtggaacaaa
     6421 tccgtatgcg ggctgtcctc atagctcaat tctgccaagg cgtcctcaaa agcaagctga
     6481 atcgactcga ccgcgatttc ggcggtgtct gcgggaaggg cgcggatcag ggctttgaag
     6541 gcggtggaca ggacgtggtt ctgcatacgc agcacatcgt ttgcttcttc cagatattcc
     6601 aaacgctgtt cgatgctgcc catgatgtat cctcgcgaat ctgttaaaat gcgccattat
     6661 atcaaactgc gctttgggcg cgggtttgcc cgcctgcaaa gattaagaac cggaagccga
     6721 aaaaatggat acaaacaatc aggataaaga gccgaattgg gaaaaacgcc tggaaaacct
     6781 gcgtcagacg cgccgtatga ataccgcgcc tgcctatgtg ccgctgacca aaccttctga
     6841 agtcggaaag gaagagccca taccgaaatt ggacgatgca aaccgagacc gtgtgttaaa
     6901 ggcgtatctg aagaaatggc aggaagaaca caacagcgcc gttgccgatt gcggcgagga
     6961 agtcgaaacc gacccgatga tcgtccttca ggaaaactgg ctcaacgcgc aggcttccct
     7021 gcaaatgccg gtttcggaaa aacacatcga atcgcggcgg agaatccggt tcgatgccaa
     7081 aaaagacagt gccgaatttg aaaccgcaca gattgagggc ggggaaaacg ccgatgcggc
     7141 agaatccggt acggcggcag acggggttgc ggaaaacggc gaaggcggtg cggaagaggc
     7201 gttgatgccc gtccaaatca atatcctgaa cccgaaagcc gtcaaccggc gcgaagtgtt
     7261 ctgcctgtcc gaacaggagc tgaccgaacg gttgatcaaa cgcctgcgcc cgcatctgac
     7321 cgataccgtc aacggcatga tccgtacggc ggtgcagaag cagatggcgc tctttactta
     7381 tcagctccag caaatgctgc acgagcaggc gggtgcggta gtcgaagacg tgttggaaca
     7441 cgatgtccgt aaaatcctca acgacatcaa gtacgaatta aaatacaaac gctgattgcc
     7501 atgccgtctg aaagccgtgt tcagacggca ttttcctgcc cgtccgactt gaaagcctca
     7561 tggaacaggc gcaaaatatt aataaattaa acaacgggtg cacgccccgt tgccgaacgg
     7621 acaaaaccga ggaatcaccg atgagcaaaa aacttcatcc gcaaacgctc gccatacgcg
     7681 gcggcaaaga acaaaccgga taccgcgaac acaatcaggc attgttcctg accagcagct
     7741 tcatgtggga caacgcccaa cacgctgccg atttgttttc aaaaaaaatc aaaggcttca
     7801 cttatacccg taccgccaac ccgaccatcg cggcctttga aaaacgcata gccgctttgg
     7861 aaggcgcgga acgcgcggtc gccacttcga ccggcatgtc cgcgattcag gcggcgtttt
     7921 tcaccttcct gcaggcgggc ggccatgtgg tttccagccg cagcctgttc ggcacgaccg
     7981 tcggctttat caataacatc gttaccaaat tcggcatcgg agtgagccgc gtgtcgccga
     8041 ctgatataaa tgaatggaaa gccgccgtca aagccaacac caaactgctg tttttggaaa
     8101 cgccgtccaa ccccttgggc gaagtggccg acttggaagc cttggcggaa ttggcgcacg
     8161 gcatcggcgc gctcttggtg gtggacaaca gcctgctgtc gcccgtcggc tcgcagcctt
     8221 tgaaacacgg cgcggatatt tccgtttcct ccgccaccaa agccatagac ggacacgggc
     8281 gcgtgatggg cggcgtgttg gcgggttcgg aagaactgat ggcgcaggtc gccgtttact
     8341 gcaactcttg cggtttggcg atgtcgccgt ttaacgcgtg gcagttgttg agcggcgtgg
     8401 aaaccctgtc gctgcgtatg gaaaaacagt ttgacaacgc cttgagaatc gcgcaatggc
     8461 tgcaagagca gccgcaggtt caagcagtgt actacaccgg attgcccgac catccgcagg
     8521 cggagcttat ccgcaaacag caaaacggcg gcggcatcgt catcggtttt gaagtcgccg
     8581 accaagcagc cgcgtggaaa gtcgtggacg gcgtggaact cttttcccgt accgccaacc
     8641 tcggcgacgt gcgctcgacc atcaccaatc cgtggacaac cacgcacggc aggatgcagc
     8701 ccgaagaaaa actcgccgcc ggtatccgcc ccggactggt gcgcctgtcg gtcggtttgg
     8761 aatatgttgg cgatttgatt gacgatttga aacaggcact cgcgcgctga ttgcgtgttt
     8821 cagacggcat cggcggaaaa atgcggcaga tgccgtctga aacccttatt gcgtatggca
     8881 tacacatcaa caataggaaa aaatatggat gaagcggttt ttgccgattg ggcgttgaaa
     8941 atctgcctga ccggtttgat tatttttttg ggttttatcg tttggaattt gggcaaggag
     9001 tccaaagcag gcaaattcgg catcgccgtt ttgtttttgg ttttgggctt gggtgtgttc
     9061 ggttttgtgt tcaaagagtt attgattaag tttttggtat tgcccaaata ggtttgccgt
     9121 ctgtcgaaat gccgtctgaa atcataaaaa gtaatctgca ccctaaaagt ttgatttaac
     9181 cgccaactga ttaaggtgcg ggtttttatg acaaaataaa aatcatcttt ccgcaatcat
     9241 tcccacggat gcgaaagcct gtctttttga atcccggata attttaca
//
LOCUS       DAXDSD010000082         9223 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXDSD010000082 DAXDSD010000000
VERSION     DAXDSD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.082"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="ACFB2Q_001630"
     CDS             complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="ACFB2Q_001630"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC0918619.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRRVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     SVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(2985..3344)
                     /locus_tag="ACFB2Q_001631"
     CDS             complement(2985..3344)
                     /locus_tag="ACFB2Q_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918620.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3343..3522
                     /locus_tag="ACFB2Q_001632"
     CDS             3343..3522
                     /locus_tag="ACFB2Q_001632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC0918621.1"
                     /translation="MPSEPAGRLSQKTPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYINNLGADKPLFI"
     gene            complement(3515..4156)
                     /locus_tag="ACFB2Q_001633"
     CDS             complement(3515..4156)
                     /locus_tag="ACFB2Q_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC0918622.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4146..4697)
                     /locus_tag="ACFB2Q_001634"
     CDS             complement(4146..4697)
                     /locus_tag="ACFB2Q_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC0918623.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4701..5219)
                     /locus_tag="ACFB2Q_001635"
     CDS             complement(4701..5219)
                     /locus_tag="ACFB2Q_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC0918624.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGKNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="ACFB2Q_001636"
     CDS             complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="ACFB2Q_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC0918625.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQVKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6873..8207)
                     /locus_tag="ACFB2Q_001637"
     CDS             complement(6873..8207)
                     /locus_tag="ACFB2Q_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HFC0918626.1"
                     /translation="MPPFIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCR
                     TDVPQLDKGEAAFCPRCGHKLFRVGSHPFSGPPAYAAASLILMAFAYSMTYIEVGIPG
                     AASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALR
                     LATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAKVRFGPAFYLMFALSVMLIRTSVSV
                     PQHWVYFQIGRLTGNNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLS
                     ISSAFLTAAVVLYFPANILPIMISSNPAATEANTIFSGIAYMWDEGDRLIAAVIFSAS
                     ILVPVLKIAAMSVLIAAARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFH
                     TYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8518..9147
                     /locus_tag="ACFB2Q_001638"
     CDS             8518..9147
                     /locus_tag="ACFB2Q_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC0918627.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
BASE COUNT         2051 a         2385 c         2634 g         2153 t
ORIGIN      
        1 agttatagtc gagatacgtg tatcggtgca accggtctgc cgttgacgaa aatgccgtct
       61 gaaagtgttt cagacggcat ttgtcttcgg ggtttcaaca gtcagtcttc ataattttcc
      121 atcggcgggc agctgcacac gaggttgcgg tcgccgtaca cgtcgtccac gcggttgacg
      181 aagggccaga acttgtgttc gcgcacgaag ggcagcggga agacggcttc ttcgcgagag
      241 tacggatgcg cccattcgcc ggttatatct gcggcggtgt gcggcgcgtt gaccagtggg
      301 ttgtcgtctt tcggccattc gccgtcgatg actttctgca cttcgcggcg aatggatttc
      361 agggcggcga tgaagcggtc gagttcggct ttgctttcgc tctcggtcgg ttcgatcatc
      421 agcgtgccgg caacagggaa ggaaaccgtc ggcgcgtgga agccgtagtc catcaggcgt
      481 ttggcgatgt cggtttcggt gatgccgctt tcggctttga gcggacgcaa atcgacgatg
      541 cattcgtgcg cgatgcggcc gtttttgcct gtatacagaa tcggatagtc ttcgctcaag
      601 cgtttagcga catagttggc gttgagcaat gcccagcgtg ttgcctgttc catgccttgt
      661 ttgcccatca tggtcaggta catccaagta atcggcagga tggatgcaga accgaaggct
      721 gcggcggcaa cggaggtttg cccggcactt gcgctgtggg tgtcggtcag ggtatgcccc
      781 ggtgcgaacg gggcgaggtg ggctttcaag ccaatcggac ccatgcccgg gccgccgccg
      841 ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca acacgtccgc gccgacttcg
      901 gcgggctgca tgatgccgat ttgggcattg aggtttgcgc cgtccatgta aacctgtccg
      961 ccgttttcgt gaatgatccg gcagatgtcg cggatgcctt cttcgtacac gccgtgtgtg
     1021 gacggatagg taatcatgat ggcggacaag gcatcgcggt gttgttcggc tttggctttc
     1081 aaatcgtcaa tattgacgtt gccgtgttcg tcggtatcga cgacgacgac tttcaaaccg
     1141 agcatggcgg cggtggcggg gttggtgccg tgggcggatt tgggaatcag gcagatgttg
     1201 cggtgtgctt cgccttgggc ttcctgataa cggcggatgg cgagcatacc gctgtattcg
     1261 ccttgtgcgc cggagttggg ctggaaggaa atcgcgtcaa agccggtgat ggctttcagg
     1321 ctgttttcca tatcagcaag caattcgcgg tagccggcgg tttgcgcttc gggagcgtaa
     1381 gggtggatgt cggagaactc agtccaagta atcggcaaca tttccgcagt cgcattgagc
     1441 ttcatggtgc agctcccgag cgaaatcatg ctgcggttca tagccaagtc gcggtcttcg
     1501 agttttttca ggtagcgcaa catttcgtgt tcggtgtggt aactgttgta cacaggatgt
     1561 tgcagaatgt cgtcttgacg cagtaattcg gcgttcagac ggcctttgac atcatcggca
     1621 aatgtgaacg tatccttgcc ggtaaacgcg cggtacaaat cggccaaatc ttcgtatacc
     1681 gacgtttcat ggaatgcagc cgcaacttga gtattgttga cgcggcgcag gttgtaaccc
     1741 gactccaaag cagcggcaaa cacttggtct gctttctctt tgctgccgaa atcgacggta
     1801 acggtatcga agaagacttc gtgaaccact ttgaggccgt ctgaaaccag cgcgtcggca
     1861 aagacggaag ccagtgtgtg aatgcggttg gcgatgcgtt tcacgccttt ggggccgtga
     1921 taaacggcgt acatgcccgc caagttcgcc agcaatgcct gtgcggtaca aatattggat
     1981 gtcgcttttt cgcggcggat gtgttgttcg cgggtggaca aagccatgcg caaggcaggt
     2041 ttgcccgatg cgtctttgga tacgccgatg atgcggcccg gggcggagcg tttgaactcg
     2101 tctttaaacg cgaaataagc ggcgtgcgga ccaccaaagc ccatcggtac gccgaaacgt
     2161 tgggtgttgc ccaaagcaat atctgcaccc aattcagccg gagacttcag caaaaccaag
     2221 ctcatgatgt cggcggcaac agcaacgatt gtgcctttgg ctttcagacg gccgataacg
     2281 tcctgcaagt cttgcacgtc gccgtcttta ccgacgtatt ggaacagcgc gccgaagtat
     2341 tcgccttcat ccgcttgggc aaaatcgctg accaccagct cgaagccgaa atatttggcg
     2401 cgggttttca tcacgtccaa agtttgcgga tacacgcgcg catccacaaa gaaacgctcg
     2461 gatttcactt tgcccacgcg gtgcgccatc gccatcgctt cggcggcggc ggtcgcttcg
     2521 tccaacaaag acgcgcccgc cacagggaaa ccggtcaaat cgatgcacac ctgctggaag
     2581 ttcaacaacg cttccaaacg accttgggca atctccgcct gatacggcgt gtaggcggtg
     2641 taccagcccg ggttttccaa tacgttgcgc aaaatcacgt tcggcacgcg ggtcgggtaa
     2701 taacccaagc cgatatagga cttgttgatc acgtttttcg acgcgatgcc tttcaatttc
     2761 gccaatgcgt ccgcctcggt cagggcttcg ggcaaatcga gttcggacgg catacggatg
     2821 ctttgcggca aggtgttgcc gacaaagtcg tccatgctct tctcgccgac agcggcaagc
     2881 aacgccgctt cgtcgccgaa actcaaatgg cgcgcggcaa attcgttggg gttgaacagt
     2941 tcggacagtt tcatttctac tccttcgggt ttgtagggct gccatcaggc agaattttta
     3001 gacatcggat gattcgggca agggtggcac aaaaggcggg ataatggcgg acaacgcgcc
     3061 agaatgcagc aggtcgggac aagcagcgcc acaagcggca gcgataaggg aaagccgccg
     3121 cccaaaacca tgaaacctgc aggcaccagc caagccaagg gatgagccgg aagcggaaaa
     3181 acaacattga gcagaaccaa aatcacgaca aacagcggca gccccgccat caggttggca
     3241 cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt cgcaatattc catgatttca
     3301 gcctgtccgg gctttatccg cagcaggttc ggacggtcgg tcatgccgtc tgaaccggca
     3361 ggccgtcttt ctcaaaaaac gccataccgc tgcaacttca tgcggctgta tggcgtaggt
     3421 tttgcacttc agacggcata tgcgtcccga agcagtttgg tgtcccagtc ggttcatgat
     3481 tatataaaca acttgggtgc ggataaacct ttgtttatct aaacccatgt tttctcaaaa
     3541 attgattcaa attttcggcg aatttccgat agcctttgcc gttggcgtgg atttggtcgg
     3601 atttcagatt attattgccc aaaatttccg cccacgcgcc gccgaacaac ggaatgccgt
     3661 attcctcgga caaatcctca tacagcggat ggtcgctcaa atgcccgaac aacgcgccca
     3721 gtgtgatgtg cggcacgccg acgaggacgg cgggaatgtt ttccttttgc acggtttcga
     3781 tgattttcgc gatattggcg cgggtctgct cctcgggaac tttgcgcaga aagtcgttgc
     3841 cgcctatgcc gacaatcaca agcttgggtt tgcgtgccaa cagcgcgggc aggcgcgata
     3901 gggcttgcgc ggacgtatcg cccgatacgc cgccgttgac aatattccaa cccgtcagtt
     3961 tttgcagttg cgcggggtag gattcgccgg ggtttgctcc gtagccgaag gtgagcgaat
     4021 cgcccaaggc aagcacggtg cttccttcgg gaattttggc gtgggttcgg gcggattttc
     4081 tgccgcaggc ggtaagaagc aacgcgcctg cgccgaggag gaaggttctt ctgttcattg
     4141 gtttttcaga cggcatcgag ccatttcggg tggtatttca gggttttacg gtaaaccgga
     4201 cagctttcgg cgtgcgcgcc tttgaggtag ccggtactca tcagaaattc gccgacgatt
     4261 tcgccgccga cgaatttgaa atgtttttta aagagtttga cccattcgtc tttgcttcgc
     4321 ggatggtgcg cgtcgagcca gttcttgaac gaaccgtgtt cttgttgcaa cgcttggatt
     4381 tgccgtgcat tgaaaatggc ggcatcgatt ttcagacggt tgcggacaat acccgcatcg
     4441 gtaagcagcc gttcaatatc ggcttcgcca aatgcggcga ccgtatcgat gtcgaaacct
     4501 tcaaatgccg tctgaaacgc ctgccgcttc ttcagcatca gcgtccaact taatcctgcc
     4561 tgattgattt ccaacaccag ccgttcaaac agtccgttgt cgtccttaat cggaaaaccg
     4621 tattgcgtat cgtggtaatg tttgttcggg ttgtcggtgt tttcgggaag tgaggcggca
     4681 aattcgcaat agttcacggt ttactcgacc atctgttgcg ccgcctgttt cagtccctgt
     4741 tcgagtgcgg cggtcatggc ggcgtagccg tcaccctgct gttcggtttc gatatggaag
     4801 ggtctgttct taccgtcggg taggacggcg tagccgctga tgagggtttt gcccgtgtag
     4861 ctgccttgga atgcgtcgat atagaccgtc catttgtcgg tactgccgct gcgtgaggca
     4921 ggaacaaagg tgcgtgtgct gtccaaacgg ttgaatgcat tgctcaacgc cgcttcgagc
     4981 atatcgtcca aggtgtctgc ccaaacatgg ttttgtgcgg tgttgatgcg gtaggggtcg
     5041 gtttgataga ccagtccgcc gcgtttgagc ggttcggcaa gacggacttc gacggcggtt
     5101 tcgccgcctt gcgttgcagg acggatgtag cggctgtcgg gcaacacgaa atattgtgtg
     5161 ctttgcacag taccgcaggc ggcaagcgtc agggcggcgg cgatggggaa aaggcgcatt
     5221 atcggcttcc tttcgggata gggtctttgc tgctgttgtt gaaaatcagt gcgttgggtt
     5281 tttctttcaa agtgttaatg acgggttgaa cgtcttttaa ggttttgtcc aaactttgca
     5341 gcgtattttg tacgtctccg tagataggcg attgaggcga tacgccttgc agggttatgc
     5401 gcaactcttt cagagtttgg ttcagttcgt tcgggatgtt ttgcgtctgc ggattgccga
     5461 ccagtttgtc aatggagctt agggcggcat tggcggattt gagtgcggac ttgagttcgg
     5521 cgagcgagcc gttcaattcg gcaacggttt tatccaatgg cagattgttg aatttgtcca
     5581 gcaaatccgc caatttgacc tgcaagtcgt ccaaaccgcc gccccgtgtg gcgatgacgg
     5641 tatcgcctgc ataaacggta tgcggtcgca gcttgggcga ggcggaaggc tgatcgttca
     5701 actcaatcat tttgccgccg gtcagcaggt tgttgctgga gatggtggcg gtcaggcctt
     5761 tgtttaaggc cgtctggaat tgttgtttcc aatgctcttt gctttgctcg tcggcattga
     5821 tttccaaacg ggaaggctcg atgcggatgc gtacgggaat ccagccgttt tcaaacaggt
     5881 gcaggctgtc attgcggtca aaataaggga catcggaaac catgccgaca ttcagccctt
     5941 tgtattcgac aggcgaaccg acggtcagtc cgcgcacgga ttgtttgaaa aacgcggtgt
     6001 agtacagcga gcggtcgtca ggcaggttgg cgatttcgct gcggctgtcg taaagcgtga
     6061 agctgtcctc gcttttgacg tttttactgt ttttggtttt cggcgaatca aatgaaatcg
     6121 cgcctgacag cagggcaggc agaggggcgg aattgagttt gatgccgctg cctgtggttt
     6181 cgatattgat gccgctttcc agccaaaaac ggctggcgga atgaatcagt ttgtcgttgg
     6241 ggctttggat gaagatggtg taatgcacgc tttggtcgga cgggtcgaaa tgcgcgcttt
     6301 cgatttgccc gaccataaag ttttcataca aaacagggct gttgacgttg aggatgcggt
     6361 cgtttttacc aatcaaattc aagcgcagcc cgctttgccc gatggcggta acgggcggaa
     6421 tgtcctgcac ttggaacacg tcttttgcct cgccgctttt gccgggtgta aaggcgatgt
     6481 acgaacccga aagcagcgta cccaaaccgg ttacgccgct ttggtcgata cgcggcttga
     6541 ccacccagaa ctgggtatcg ctgcggatga ggccggatac gtccgcattg agttgggcag
     6601 taacttccac gcctttttgg tcgtcgcgca gtttgattcg ggtaacgcgt ccgacatcga
     6661 tgctcaatac cttaatgacc gtattgttga cttcgatgcc ttccgcgctg tccatcaaga
     6721 gcgtaaccac aggccccctg ttgcggattt ccttaaccca aagccagccg ccggcaatca
     6781 gcgcgatcag cgggaccagc cagacggcgg agaggaaggt gttgtttttg cggacgcgtg
     6841 cttgagcgtg tccgtttgga ggagggctgt tgtcagtcat atttttccgt ttcgttgaaa
     6901 gcaatgccgt ctgaagcgcg tttgtcccaa agcaggcgcg ggtcgaaata ataggcggac
     6961 agcatcgtca aaatcacgac caggcagaaa tagactgccg cactgcccgg aatgacgcgc
     7021 gcggcataag tgtggaacga acacatcaaa ataataatca caaaaatatc aatcatcgac
     7081 cagcggccga ccgcttcggt gatgcggtag aggtgcgaca atttctttgc gcccgccggc
     7141 aaagcgaacc gtgccgccgc aatcaaaacc gacattgccg caatcttcag caccggcacc
     7201 aaaatactcg cgctgaaaat aaccgccgca atcagcctgt cgccctcgtc ccacatataa
     7261 gcgatgccgc taaagatggt gttggcctcc gtggcggcag gattggacga aatcataatc
     7321 ggcaggatat tggcagggaa atacaaaaca accgccgccg tcagaaacgc cgacgaaata
     7381 ctcagacttt tcggccgtcg gcggtacagt tccgcgccgc acaccccgca gggggattcg
     7441 gcactgtcgc ggaaatacag gcagcggctg caacaggttt tgccttccga tgccgtctga
     7501 accgcattat tccccgtcag ccgcccgatt tggaaataca cccaatgctg gggaaccgat
     7561 accgaagtcc gaatcagcat aaccgacagc gcgaacatca gataaaacgc cggcccgaag
     7621 cgaacctttg ccacagacga gagcttgata tacgccacca gagtggaaac aaaaaacaca
     7681 tccaccatca tcgcctgcct caagcgcacc atcacacgcg ttgccaaacg cagcgcagga
     7741 tacgcctgtt tccgtatcag cgcggcatag acatacaggc acagcagcag aaacagaacc
     7801 ggcgcgccga aagtcagcac aaacatcact tcggccaaaa aaccataatc ctgaaacacc
     7861 atcaggcgca tcatctcggg cagcgaaagg acggatgccg cacccggtat cccgacctcg
     7921 atatacgtca tactgtaagc aaacgccatc aaaatcagcg aagccgccgc ataggcgggc
     7981 ggaccggaaa aagggtggct gcccaccctg aagagtttgt gtccgcaacg gggacagaac
     8041 gccgcctctc ccttgtccaa ctggggcacg tctgtacggc agccgcattc cgggcaatct
     8101 accgtatgcg gcggcagggc ttcgtcgcgg cagttgccgc gaaggctgtg ggggatattt
     8161 tcggcaaacg gcttcatcgg gaaagtggca aatataaatg gcggcattat aaaggaaaaa
     8221 aagcgataca cccaatcagg atgcaaaacc ggcaggttcg gcagtatgaa caaacccttg
     8281 gtgtccggca aaaatatttt ttgagcggct gcccctccgg ccgacaagcc tatttgataa
     8341 aaccccgatt ttgtaaataa tcataaaata cccgcttaaa aatataacgc aaatgatatt
     8401 ttttcgtaaa atatgtgata ataaatttcg tttatgattc tttattattt ttgtatgcgg
     8461 gcaagtccgg attttgcccg caccgcatcc ttatccctta ccgacaaagg agaacttatg
     8521 agcgaaaccg aaaaccaagc cctgacgttt gccaaacgcc tgaaggcgga taccacggcg
     8581 gttcacgaca gcgtggataa cctcgttatg tctgtccaac cgtttgtcag caaagaaaac
     8641 tacatcaaat ttttgaaact gcaatccgtg ttccacaaag cggttgatca tatctataaa
     8701 gaccccgagt tgaacaaagc catttccgag ctggaataca tggcgcgata cgatgccgta
     8761 acgcaagact tgaaagactt gggcgaagag ccttacaaat tcgacaaaga attgccacac
     8821 gaaaccggca acaaagccgt cggctggctt tattgcgccg aaggatccaa tttgggcgcg
     8881 gcatttttgt tcaaacacgc gcaaaagctg gattacaccg gcgaacacgg cgcgcgccac
     8941 ctcgctccgc atccggacgg gcgcggcaaa cactggcgcg ccttcgtcga gcacctgaat
     9001 gccctgaact taactccgga agcagaagca gaagccattc aaggcgcgca ggaagcgttt
     9061 gctttctata aagtgattct gcgtgaaacc ttcggtttgc cggaaggaac agaagccccc
     9121 gaaggcatga tgccgcacag acattaaaaa tggggctgta ctagattatc cctaaattcc
     9181 acaccgatcc cgcaggattt ttagctgccg ggacggtgtg ccg
//
LOCUS       DAXDSD010000083         9212 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXDSD010000083 DAXDSD010000000
VERSION     DAXDSD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.083"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..1067)
                     /locus_tag="ACFB2Q_001639"
     CDS             complement(<1..1067)
                     /locus_tag="ACFB2Q_001639"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFC0918628.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTAILLREMSGGRFFLKTFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFFL
                     LWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVAR
                     KSLRVQFGFLAALCALSLAASFMPSALDKYYLPHLRACEMLVGSLTAVRMRYRQQRNP
                     AVGKRYAAVGALFSACILSACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLK
                     KFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYH
                     CIEKPFKKWKGSFAQSVLWIYA"
     gene            complement(1064..1303)
                     /locus_tag="ACFB2Q_001640"
     CDS             complement(1064..1303)
                     /locus_tag="ACFB2Q_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918629.1"
                     /translation="MPSEAGFGGFRRSHTAGMQPHQNKIPPAFPICPPRFLQNKPPAL
                     PLREAVRYSEYPEPRYKMTFQTAFAQPAAFQVKTL"
     gene            complement(1341..2432)
                     /gene="ychF"
                     /locus_tag="ACFB2Q_001641"
     CDS             complement(1341..2432)
                     /gene="ychF"
                     /locus_tag="ACFB2Q_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC0918630.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(2546..4222)
                     /locus_tag="ACFB2Q_001642"
     CDS             complement(2546..4222)
                     /locus_tag="ACFB2Q_001642"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC0918631.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            4397..4827
                     /locus_tag="ACFB2Q_001643"
                     /pseudo
     CDS             4397..4827
                     /locus_tag="ACFB2Q_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(4861..5556)
                     /gene="murU"
                     /locus_tag="ACFB2Q_001644"
     CDS             complement(4861..5556)
                     /gene="murU"
                     /locus_tag="ACFB2Q_001644"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC0918632.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(5572..6072)
                     /gene="hpaC"
                     /locus_tag="ACFB2Q_001645"
     CDS             complement(5572..6072)
                     /gene="hpaC"
                     /locus_tag="ACFB2Q_001645"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC0918633.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            complement(6187..6627)
                     /gene="nadR"
                     /locus_tag="ACFB2Q_001646"
     CDS             complement(6187..6627)
                     /gene="nadR"
                     /locus_tag="ACFB2Q_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC0918634.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(6968..7477)
                     /locus_tag="ACFB2Q_001647"
     CDS             complement(6968..7477)
                     /locus_tag="ACFB2Q_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC0918635.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIAATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            complement(7557..7632)
                     /locus_tag="ACFB2Q_001648"
     tRNA            complement(7557..7632)
                     /locus_tag="ACFB2Q_001648"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7597..7599),aa:Lys,seq:ttt)
     gene            7851..8930
                     /gene="apbC"
                     /locus_tag="ACFB2Q_001649"
     CDS             7851..8930
                     /gene="apbC"
                     /locus_tag="ACFB2Q_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC0918636.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVGQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPLTGDTHIHLSIDTEIGTHKVRPGVATIKGVKNIIAVA
                     SGKGGVGKSTATANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT         2122 a         2637 c         2464 g         1989 t
ORIGIN      
        1 gcataaatcc ataaaacgga ttgtgcgaac gagcctttcc attttttaaa cggcttttcg
       61 atgcagtgat aagaaatcag ggaaaacgcc agggtcagga cgatcgctgc cgccggcgaa
      121 taaggcggca ggttgtccgg gccgatatag cgcataaagg ccaatatcgg ccaatgccac
      181 agataaagcg aataggaaat caaaccggcg gcaacggtga ttttccattg gaaaaatttt
      241 ttaagcgggt gttcgtaatg gttgaaataa atcagcgcgg caacagccag acagggaatc
      301 aaagcggcgg ggcccgggaa ataggcggtt tgttccgaat aggcaaacag gcaggcggac
      361 agtatgcacg cggaaaacaa tgcgccgacg gcggcatacc gtttcccgac ggcgggattc
      421 cgctgttgcc ggtaccgcat ccgcacggcg gtcagcgatc cgaccagcat ttcgcaggcg
      481 cgcaggtggg gcaggtaata tttatcgagc gcggaaggca taaaggaagc ggcaaggctt
      541 aaggcgcaca atgcggcgag gaagccgaac tgtacgcgca ggcttttgcg ggcgacaagc
      601 aacagcaata tcggaaagac aaagtaaaat tgttcttcga ccgacaaaga ccagatgtgc
      661 agcaggggct tttcttcctg cgcgggatcg aaataatcct tcccccttgc aaaatacagg
      721 ttggaggcga aacccaaggc ggtcagcgcg gatttccaca aaagaaagaa atcatctttg
      781 gtgaataaaa agaagccgcc tgccagcgtt gccgccaata cggcgaaaaa tgcgggcaaa
      841 atccgtttga tgcggcggat ataaaatgtc ttgaggaaga aacgcccccc ggacatttcg
      901 cgaaggagga tcgccgtcat caaaaagcct gaaatcacaa agaatatatc gacaccgaga
      961 aacccgcccg gcagccaatc cttttcgata tggaacacga tgacggacaa gacggcggcg
     1021 gcgcgcaatg tgtcgatgtc cgggcggtag ggtaaggctt ggctcataat gtttttactt
     1081 gaaacgcggc gggctgtgca aatgccgtct gaaaggtcat tttgtatcgg ggttcgggat
     1141 attcggaata ccggacggct tcccgtaacg gcagggcagg cggtttgttt tgcaggaatc
     1201 ggggagggca aatcggaaat gcgggcggga ttttattttg atgcggctgc attccggcgg
     1261 tatgggaacg tcgaaaacca ccaaaaccgg cttcagacgg catttccggc aaagccgtct
     1321 gaaaacctgc cgcatttggg ttacacgtta aacaaaaagt gcatcacgtc gccgtcctgc
     1381 acgacgtatt ccttgccttc cacgcgcatt ttgccggctt ctttggcttt ggcttcgccg
     1441 ccgagcgaga caaaatcatc gtaggcaatc acttgggcgc ggatgaagcc gcgttcaaaa
     1501 tccgtatgga tcacgccggc ggcttgcggc gcggtgtcgc ctttgtgtat cgtccacgcg
     1561 cggacttctt tcacgccggc ggtgaagtag gtttgcagtc ccaaaaggtc gtaacccgcg
     1621 cggatgaggc ggttcaagcc cggttcttcc aagcccattt cggcgagaaa ttcggctttt
     1681 tcaccgtctt ccaattcggc gatttcgctc tccatcgcgg cgcagacggc aacgacgggg
     1741 gcgttttctt ttgccgccaa ttctttcagg cggtcgaggt gcgggttgtt ttcaaaaccg
     1801 tcttcggcaa cgtttcccac atacatcgca ggtttggcgg tcagcaggaa cagcggcttc
     1861 agcagcgcgc gttcttccgc gtccaaaccg aaggaacgca cgggtttgcc ttcgtccaga
     1921 tgcggcagca gttttttgca caaatcgact agtttttgcg cgtctttgtc gcctgatcgg
     1981 gcgcgttttt cttcgcggac gatggctttt tccacacttg ccaagtcggc aagcgccaat
     2041 tcggtgccga tggtttcgat gtcggcaatc ggatcgactt tgccggaaac gtggacgatg
     2101 ttgtcgtcgt caaagcagcg cacgacgttg acgatggcat cggtttcacg gatgttggca
     2161 aggaaccggt tgcccaagcc ttcgcctttg ctcgcgcccg caaccaagcc ggcaatatcg
     2221 acaaactcga cgatggcggg ctgcattttt tgcggattga cgattttcgc cagctccgcc
     2281 atacgcgggt cgggcacttc gacgatgccg acgttgggtt cgatggtgca gaagggatag
     2341 tttgccgctt cgatgcccga ttgggtcagc gcgttaaaaa gggtggattt gccgacgttg
     2401 ggcaaaccga cgatgccgca tttcaaactc atggtttttc ctgaaaatag agaaatttaa
     2461 cggcggatta tagcataccg ccgcccgcgt tccgaaaaaa tgccgtctga aacggcttca
     2521 gacggcatcc ggtttcaaaa accgttcaga acaagccgtg aatcacgccg tgttcgtcca
     2581 catcgatttt ctcggcagcc ggaactttcg gcaggcccgg cattttcatc atattgccgc
     2641 acaacgcaac gatgaagccc gcgccggcgg aaacagtgat accgcgtacg gtgatgcgga
     2701 agccttcggg gcagcccaag agtttggcgt tgtcgctcaa tgaatattgg gttttcgcca
     2761 tgcagatcgg cattttgtcc aagcccagtt tttccagcga ggcgatttcg gcagacgctt
     2821 ccgcgctgaa atcgacatct tccgcgccgt acactttttg ggcaatcgca cggattttgt
     2881 ctttgatgcc caactcgaca tcgtaggcga aaccgaagtt attaggttgg ttgtcgatgg
     2941 cattgacgac tttgcgcgcc aaatccgcgc cgcccgcgcc gcctttgccc cacacttcgg
     3001 tcagggaaac ttcaacgccg tgttcggcac aggctttttc aatcatcgcc aactcggcat
     3061 cggagtcgga cacgaagcgg ttgagcgcaa cgacgacggg cagtccgaat acgtttttca
     3121 ggttggaaat gtgtttcaac aggttgggca aaccttttgc caaggcttcg aggttttctt
     3181 caccaaggtt ggcgcgttcc acgccgccgt tgtatttcag ggcgcggaca gtcgccacga
     3241 cgactgccgc atcaggtttc aaaccggcaa ggcggcattt gatgtcgcag aatttttccg
     3301 cacccaagtc cgcgccgaag cctgcttcgg ttacggcgta atcggcaagg tgtttcgcca
     3361 gacgggttgc ggtaacggag ttgcagccgt gggcgatgtt ggcgaacggg ccgccgtgta
     3421 caaaggccgg agtgccttcg atggtttgca ccaaattggg cttaatcgca tcttttagca
     3481 atgccgccat cgcgccgtgt gccttcaaat ctttggcgta aacggggctg ccgtctttgg
     3541 cgtaggcgac gagaatattg ccaaaacgct ctttcaaatc gctgatgtct ttggcaaggc
     3601 agaataccgc catcacttcg gaggcgacgg tgatgtcgaa gccgtcggga cgcatcacgc
     3661 cgtcaacagg cttgcccata ccgtcgatga tgttgcgcaa ctggcggtcg ttcatatcga
     3721 ccacgcgccg ccacagcacg cgtttggggt cgatgttcaa ctcgttacct tggtagatat
     3781 ggttgtcgag catggcggcg aggaggttat tcgccgcacc gatggcgtgg aagtcgccgg
     3841 tgaagtgcag gttgatgtct tccatcggca aaacttgcgc gtagccgccg cctgccgcgc
     3901 cgcctttcac gccgaacacc ggacccaaag aaggctcgcg caaagcaatc acagagtctt
     3961 tgccgatatg gcgcaatgcg tccgccaaac cgatggttac ggtggttttg ccttcgcccg
     4021 ccggagtcgg gttgatggcg gtaaccaaaa tcagcctgcc ttgtttttgc ggcagcttga
     4081 acgcttcggc aggattgatt ttggctttgt aatgaccgta aggctcaatg ttgtcaacgt
     4141 tcaaacccag cttggcggca atttcgccaa tcgggcgcat ggtggaggat tgggcggttt
     4201 cggcatcggt tttgaagctc atgattttcc tttagaaatg aggagggaca tgccgtctga
     4261 aagcatcagg cgacaaacag gtggattgaa aataatatca ggcatattat aacgttatcc
     4321 gcaccaaacc cgcagtgaaa tttttgacgc agcaacaaaa ataccgttca tattgttcac
     4381 aatcaaagga gaaaacatgg gcagcaacgc atggctgttt tgggcattgg catcggcagg
     4441 cttcgcctca ttgaccgccg ttttcgccaa aatgggcttg cagggcatag attcggattt
     4501 cgccgccttt atccgcacct tggtcatcct cgccgccttg gtattgtttt tgacctacac
     4561 cggcaaatgg cagggcgtaa acggcttcac ggggcgcaac cggacgtttc tggttttgtc
     4621 cggactcgcc accggcgcat cttggctcgc ctacttcaaa gccctgcaac tgggcaacgc
     4681 cccgcaagtc gcgcccgtcg acaaattcag cctggtcttg gtcgcgctga tggcggtgat
     4741 tttcttagac gaacgcccga acacgcagga atggatagac ttggggctgg ttacggcggc
     4801 gtgttgacgt tggcactgaa acgttaaacc gaatccgcca taccgcctga aaccgagttt
     4861 tcacttccaa gccgctgcaa gggcttgcgc ttccttcaga cggcataccg tgccgacatc
     4921 cagccacagg cccgtatgct tctgaccgct cacgcggttt tgccgcattt cgttcagcaa
     4981 tacgggcgcg agttttgcca cactgcccgc ttcgattccg tcaaacattt caggacggta
     5041 aatacccacg ccgctgaatg tcagtccgtt gccgccggat acttccggcc gcacgctgcc
     5101 gtcgggcagc agggaaaaat cgccgtcggg gttgtgcggg ggatttccca ccagccacaa
     5161 atgtgcggaa atgtgcccgg gcagggacga tgccgtctga aacgcggcgg taaaatcgat
     5221 gtcggtcagc acgtcgccgt tggccaccaa aaacggctgc ccacccaaca gcggcaatgc
     5281 ctgcgcgatg ccgcctgccg tttccaaacc gcctgcgggt tcgggcgaat aggcgatgtt
     5341 cacgccataa gccgagccgt cgcccaaagc attttctatc tgccgaccca gccaagcgtg
     5401 gttgatgacg atttcggtaa accccgcctg cttcagacgg cacaagtgcc aaccgattag
     5461 aggcttaccc gccacatcga gcagcggctt cggagtggtg tcggtcaaag ggcgcatacg
     5521 ctcgccgcgt cctgccgcca gtatcatcgc tttcatatat ctgtccgaat atcagtctaa
     5581 aggcctaaac tgccgtctga aatacagcag cgcggggcgt ttgcacccgc agtttttgat
     5641 ttcgtcgagc ctgacgtaaa acacaaaatg cgtgccgatt tcatgtttgc cgacaatatg
     5701 cccgtgcagg tgcgccaacg cgccttctat ttcaagttgt cccgttttgc cgcgatgcca
     5761 aatatggtag gcaaaccgct cttcgggcga caggccggtc agcccggcaa aatgttcggc
     5821 aacatcctga tgttcgtccg ccagcatatt gatgcagagg ctgccgtttt ccgacaggat
     5881 cggaatgatt cgcgcactcc ggtttatgca cagcatcacg gtcggcggct cgtcggtaac
     5941 cggcgcaacc gccgtcattg taatgccgta acgccctgcc gcaccgtctg tcgtgatgac
     6001 atgaacgcct gccgcacagg atgccatcgc atcacggaac gaagtttgaa aagttttctg
     6061 caaatccgcc atttttcccc tttaaaccgt cccctatata agaatgctgc acacaaggca
     6121 tcccccatgt gcagcagttc tgattcaaaa agccgtcggt cggacgtttc cgcgcattac
     6181 ggcgtattac gagttcaacg catcctcgat tttggcaagt tctgccaaca ggtctttaag
     6241 cagcagcatt ttctcgcggc ccagcacttc ctcgatagcg tcgtaacgct cgtccacttc
     6301 ttcgccgatt tcctcataca gcttctcgcc ctcggaagtc agcttcagat aaacgcgccg
     6361 ttggtcgttg gaaggtttca ggcggacgac caaccccgct ttttcaaggc gggtcaggat
     6421 accggtcagg ctggggcgca aaatgcacgc ctgattcgcc aaatcctgaa aatccaatgt
     6481 gccgttttcc gccaaaagac ggataatccg ccattgctga tcggtaatat tcgcctgatt
     6541 cagaataggc ctgaattggg tcatcagggc ttcccttgcc tgtatcagac cgatattgat
     6601 agacgcatgt tttgattggg taggcattgt ttaagtctcc aagttatcga aaatcaaact
     6661 ttcaaaccgt cgggaaagcc tgtgggcgta atttttgatg caaccgttat ataacaaaac
     6721 gaacatatag caacaatacg ctataaacct catcggacga ccgggtataa aagactttaa
     6781 ttccgataat cctatctgaa aatattttaa tagttatatt ttaatctatt tttcccacaa
     6841 tcacaacaag ggattacatc ggcaggcgcg ccggctcttt cccaaaaaac aaaagccgcc
     6901 gcatccgccg cgcaaggcat atgccgtttg attctctaca tagcggaaag tttgacaaaa
     6961 acaaaagtta accgaaaaaa tccgcctgaa aaattcgtgc gcgcaagccc caataactgc
     7021 tgattcccgt cgtatagcgg accattttcc catttttgac caaaacgacg gcaggcgttg
     7081 cggcaatccg ccaagacctt gccaaacccc cgtcctcatc gctgacagtc ggaaagccca
     7141 agccccgttt tgccatatat gccgccactt cggacgcgct gccggaacgt accgccacac
     7201 cgacgaccgg cacgccgtcc gccgccaaat catcgattat cggcgactga taacggcaca
     7261 cgccgcacca gcttccccaa aaatacacca gcaccgtcct atcccggcta aactgtccca
     7321 aagtcagccg ctgccccgac agcagggtca aaggccgccc tgccgccccg gcaggttcgt
     7381 cgggcttgcg tacccaatcc aaaaacagcg acaccagcaa aaacaccaat gccgtccgaa
     7441 cggcaaattt gatgcccgaa agcagtttct ttttcataca cccccgcaaa cggtacgccc
     7501 gcgcaaccgg caggcaaaca aaaaagccaa gtctcgaaac ttggcttccg gttatctggt
     7561 gggtcgtgag cgattcgaac gctcgaccaa cggattaaaa gtccgctgct ctaccggctg
     7621 agctaacgac ccgataagcc gtgcattata cagccccgtt ccccgccgtc aagcaaattt
     7681 tacaggctta attgcagact gctgtttgca cgggatattt tggcaaccga ttttcacaat
     7741 ccgccgcata ccgtgtaaaa gttcgcacaa gaaaaaagca aactgcccga aatcatgtac
     7801 actttccgtc tatcttcccc tcacacgact tgcgaacaga ggcacacatc atgaatatac
     7861 aaaacatccg caccctcctc gacaccgtcg ccgttccgaa tacggcacgc acgctcggcg
     7921 gcgaaaaggc cgtccgctcg gtcggacagc gttcagacgg catccacatc gccctgcatt
     7981 tcggcttccc tgtcgcgcac atcgccgccg ccctcgccga tgccgtacaa gaaaccctga
     8041 tgccgctgac aggcgacaca cacatccatc tgtccataga caccgaaatc ggcacacaca
     8101 aagtccggcc cggcgtcgcc accatcaaag gcgtgaaaaa catcattgcc gtcgcatcgg
     8161 gaaaaggcgg cgtgggcaaa tcaaccgcca ctgccaacct tgccgccgca atggcgcgca
     8221 tgggcgcgcg cgtcggcgtg ctcgatgccg acctttacgg tccgagccag cccacgatgc
     8281 tgggcgtaca cgaccgcaaa cccgatcaga aaaaccaaaa actcattccc gtcgaatctt
     8341 cagacggcat acaggttatg tccatcggct ttctcgtcga taccggccaa gccgtcgtct
     8401 ggcgcggccc gatggtcagt caggcattgc agcagctgat gttccaaagc gagtgggacg
     8461 aagtggacta tcttttcatc gacctgcccc ccggcacggg cgacatccaa ctcacgctgt
     8521 cccaacgcat ccccgtaacc gggtccgtca tcgtaaccac gccgcaggac atcgcgctga
     8581 tagacgcgcg caaggctgtg gatatgttcc gcaaggtcaa cattcccatt ttgggcgtat
     8641 tggaaaacat gtccgtccac atctgctcca actgcgggca tagcgaagca ctgttcggca
     8701 cggacggcgg caaagatttg gcggcccgcc tcaacgtccc cctgctcgga cagcttcccc
     8761 taagcctgcc cgtgcgcgaa gccatggacg gcggcacacc ggcgcaactg ttcgacgaac
     8821 accccgccat cgcccgaatc tacaccgatg ccgcattcca aatcgccctg ggcattgccg
     8881 acaaaggcaa agacttcagc agccggttcc ctaaaattgt cgtcgaataa agccgcgccc
     8941 gaaaccgcaa cagcaatgcc gtcccaagcc ccgcgcctgc cgacgggctt catagtcctc
     9001 tctcccgtgg gagagggctg gagagagggc agcaagccgc aaggcttgta tttgggcggt
     9061 taagttgttg ggaaaggttg ccgcaacttg gagaatgccc tctccccggc cctcccccac
     9121 gggggaggga gtgggttgcg gcaggttttg cggttgcagg cggtttgaaa aacagattgg
     9181 atttacaacc gttgatgcag gtcgtctgaa aa
//
LOCUS       DAXDSD010000084         9125 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXDSD010000084 DAXDSD010000000
VERSION     DAXDSD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.084"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..377
                     /locus_tag="ACFB2Q_001650"
     CDS             <1..377
                     /locus_tag="ACFB2Q_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC0918637.1"
                     /translation="GGGCHAGITVVCGPFAVIRVMSGENELRQMKPGAVLINCGRGGP
                     VDENALPAALKYGQIGGAGADVLTQEPPRGGNPLLNARLPNLIVTPHTAWASREALDR
                     LFEILLANIHAFVKGEAQNRVV"
     gene            complement(452..2506)
                     /gene="metG"
                     /locus_tag="ACFB2Q_001651"
     CDS             complement(452..2506)
                     /gene="metG"
                     /locus_tag="ACFB2Q_001651"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC0918638.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELFNPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGGETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKAGSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWKNTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(2622..4460)
                     /gene="glmS"
                     /locus_tag="ACFB2Q_001652"
     CDS             complement(2622..4460)
                     /gene="glmS"
                     /locus_tag="ACFB2Q_001652"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC0918639.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVYSDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNAAEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            4732..5238
                     /locus_tag="ACFB2Q_001653"
                     /pseudo
     CDS             4732..5238
                     /locus_tag="ACFB2Q_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5542..6867)
                     /locus_tag="ACFB2Q_001654"
     CDS             complement(5542..6867)
                     /locus_tag="ACFB2Q_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC0918640.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKRFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTERARFPIY
                     GIPDDFISVPLPAGLRGGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSGNEGPVGALGTPLMGEYAGAIDRHYITLGAPLFVATAHPVTRKVLNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            7100..7919
                     /gene="efeU"
                     /locus_tag="ACFB2Q_001655"
                     /pseudo
     CDS             7100..7919
                     /gene="efeU"
                     /locus_tag="ACFB2Q_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
     gene            7916..9082
                     /gene="efeO"
                     /locus_tag="ACFB2Q_001656"
     CDS             7916..9082
                     /gene="efeO"
                     /locus_tag="ACFB2Q_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC0918641.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEV
                     KELAAKTKTFTEAVKAGDIEKAKSLFAATRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEAAGSKISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
BASE COUNT         1883 a         2282 c         2837 g         2123 t
ORIGIN      
        1 tcggcggggg atgccacgcc ggcattaccg ttgtttgcgg gccatttgcc gttattcggg
       61 taatgtccgg cgaaaacgag ttgcggcaga tgaagcccgg tgcggttttg attaattgcg
      121 ggcgcggcgg gccggtggat gaaaacgcgc tgcctgccgc actcaaatac gggcagatcg
      181 gcggggcggg cgcggatgtt ttgacgcaag aaccgccgag gggcggcaat cccttgctga
      241 atgcacgatt acccaatctg attgttacgc cgcataccgc gtgggcgagc cgcgaggctt
      301 tggatcggtt gtttgaaata ttgttggcga acattcacgc ctttgtgaaa ggcgaggcgc
      361 aaaaccgcgt ggtttgaacc tgtcgggatt gcggaaaaaa acgccgtctg aacgcccaag
      421 gggttcagac ggcgtttttt gagatttctg cctaaccgac tttgtcgccc ggctgagcgc
      481 ccgcatccac gtcaagcagc ttcagtttgc cgtctgccgt ggcggcggag aggatcatac
      541 cttcggatac gccgaatttt gccattttgc gtggggcgaa gttggcgact gcgatgacca
      601 tgcgaccgtt caattcggca ggatttgggt aagacgcggc gatgccggag aagatgatgc
      661 gtttttcaaa accgaaatcg aggtcgaatt tcaaaagttt ggtgctgcct tcgacggctt
      721 cgcagttcaa tactttggcg acgcgcatat cgattttcat aaagtcgtcg aagcttgcct
      781 gttcggcgac tttttcgtat ttaccctctt caacgggcgc ggatgccgtc tgaatgcttt
      841 gtttgttggc ttcgatcaaa tcgtccactt gtttttgctc cactcgttgc attaaatgtt
      901 cgtatttgtt gatggcgtgt tcgcctaggg tttcgcgcgt gttcttccag gtaatcgcgt
      961 ccagattgag gaaacgcgcg gcgttggcgg cggtttgcgg caacacgggg gcgaggtagg
     1021 cggtcagcat ggtgaaggcg ttgatgagtt cgctgcatac ttcgtgcagg cgttcgtctt
     1081 gaccttcttg tttggcgagc tcccacggtt tgttggcatc gacgtattcg ttgacaatgt
     1141 ctgccaatgc catgatgtcg cgcagggcgc gggcgtattc gcggttttcg tattgctcgg
     1201 cgatggtgtc gctttcggcg gcgagttttg ccagcaatgc gctgcctgaa acgtctttcg
     1261 gacgaccttc aaagcgtttg gcgatgaaac ccgaagcgcg ggcggcgatg ttgacgtatt
     1321 tgccgacaag gtcgctgttg acgcgcgaaa taaagtcttg caggttcaaa tcgatgtctt
     1381 cgattttgct gttgagcttg gcggcgatgt agtagcgcat ccactcgggg ttcaggcctt
     1441 gttccaaata ggatttggcg gtgatgaagg tgccgcgcga tttggacatt ttttgtccgt
     1501 ccacggtcaa aaagccgtgt gcgtacacgc cggtcggggc gcggtggccg gagaaatgca
     1561 gcatggccgg ccagaacagg gcgtggaaat agaggatgtc tttgccgatg aagtggtaca
     1621 tctcggtttg gctgccggct ttgaagtatt cgtcaaaatc gatgccgatg cggtcgcaca
     1681 ggtttttaaa cgatgccatg tagccgacgg gcgcgtcgag ccagacgtag aagtatttgc
     1741 ccggcgcgtc ggggatttca aaaccgaaat acggcgcgtc gcgggaaatg tcccagtcgg
     1801 acagggtggt ttctccgcct tcgcccagcc attccttcat tttgttgagg gcttcgggtt
     1861 gcagatgggg cttgccgtcg tgcgggttgt tgccggaagt ccatgctttg aggaagtcgg
     1921 cgcattcgcc cagtttgaag aagaagtgtt cggattcgcg caattcgggt ttcgcgccgg
     1981 aaacggcgga atacgggttg aacagttcgg tcggggaata ggtcgtgccg cagacttcgc
     2041 agttgtcgcc gtattggtct tgggcgtggc atttcgggca ttcgcctttg acgaagcggt
     2101 cgggcaggaa catttgtttt tcggggtcga aaagctgctc gatgacgcgg ctttcgattt
     2161 tgccgttggc tttcagcgcg cggtaaatgt cttgggaaaa ctgtttgttt tcagtggaat
     2221 gggtgctgta ataattgtcg tagccgatga aaaagccggt aaagtcggca aggtgctctt
     2281 tgcgcacttt ggcaatcatg tcttcgggcg cgatgccttg tttttgcgcg gcaagcataa
     2341 cgggcgtgcc gtgggtgtcg tcggcgcagc agtagtagca ttcgtggccg cgcagttttt
     2401 gaaagcgcac ccaaacgtcg gtttggatgt gttcgaccat gtggccgagg tggatgctgc
     2461 cgttggcata gggcagggcg gaggtaacta agattttgcg tgtcatattg tgctttgcaa
     2521 acaatgggta aaggcggatt ataccgcaaa tcaaacgggg aaatgccgtc tgaagcctga
     2581 aaaatcgggc ttcagacggc atttttgcca accggcggga tttattcgac ggttacggat
     2641 ttcgccaggt tgcgcggctt gtccacatcc gtgccgcgcg cgagggcggt gtggtaggcg
     2701 aggagctgca cggggacggt atgcacgacg ggggacagtt cgccgacgtg gcgaggtgcg
     2761 cggataacgt gcacgccttc ggcggcatta aaattgctgt cgaggtcggc aaagacgaaa
     2821 agttcgccgc cgcgcgcgcc gacttcctgc atattggctt tgactttgtc caacaggctg
     2881 tcgttgggcg cgatgacgac gacgggcatg ttttcgtcca ccaaggcaag cggcccgtgt
     2941 ttcagttcgc cggcaggata ggcttcggcg tggatgtagg tgatttcctt tagcttcaac
     3001 gcaccttcaa gggcaatcgg gtaatggatg ccgcgcccca aaaacagcgc gctggttttc
     3061 ttggcaaact gttgcgccca agcggcaatt tgcggttcga ggttcaaagc gtgctgcacg
     3121 ctgccgggaa gctggcggag ttcttcggtg taacgtgctt catcttcgcc ggataccacg
     3181 ccgcgcactt tcgccagcgt taccgccaaa ccgaacagca cgaccagttg cgtggtaaat
     3241 gctttagtcg aggcgacgcc gatttccgca ccggcacggg tataaagcac aaggctgctt
     3301 tcgcgcggca gggcggattc catcacgttg cagacggaga ggctgtggcg gtgtcccaaa
     3361 gatttggcgt atttcaaagc ctccatcgtg tccagcgttt cgccggattg ggaaatggta
     3421 atgaccagtt ggtcggggtc ggcaatcacg ctgcggtacc ggtattcgct tgcgatttcg
     3481 acgtcggccg ggattttggc gatggattcc aaccaatatt tggcggtcag cgcggcgtaa
     3541 taggacgtgc cgcaggcaag gattttgacg ctgcggatgc tttcaaacac gcttttggcg
     3601 tttttgccga agttttcggg gatgaatccg ccgtcgagga aaacttccgc cgtgtccgca
     3661 atcgcgcgcg gctgctcgtg gatttctttt tgcataaagt ggctgtgcgg acccggttcc
     3721 aaagaggcgg gcgagagttc ggataccttg actttgcgtt cggcaggcag gccgttttta
     3781 tcggtcagcc gtttgatgcc gtctgaagcc agcagcgcga tgtcgccgtc ttcgaggtac
     3841 gccacgcggc gcgtaaaggc gatgacggcg gatacgtccg aagcgataaa ggtttcatcg
     3901 tcgcccaaag cgaccaaaag cgggcagccc atacgcgcca caactaattc atcaggcttg
     3961 tcttgggcaa taaccgcgat ggcgtatgcg ccgtggaaac gtttgaccgc ttcctgcacg
     4021 gcttcaaaca atttgccgcc gttttgcgcg tattcgtgat tgatgctgtg tgcgatgact
     4081 tcggtatccg tttgcgattc aaaccggtat cccaaacctt ccaagcgttt gcgttcgctt
     4141 tcaaagtttt cgatgatgcc gttgtggacg acggcaatca taccgccgct gatgtgcggg
     4201 tgggcgttcg gctcggtaac gccgccgtgt gtcgcccagc gcgtatgccc gatgccgatg
     4261 ccgccgctga tgcctttttc gcgtgccgcg tcctccataa gctgcacgcg tccgacgcgg
     4321 cgcacgcgct tgattttgcc gtctgaatac acagcaatgc ctgatgagtc ataaccccgg
     4381 tattcgaggc gtttgaggcc gtcggtcaga aaatcgacga cgttgtgatg ggcgcggatg
     4441 gcgccgacga taccgcacat aaactgttcc ttagtatccg gttgaaaaaa aagatgcgga
     4501 cggcttccgt accgcacctt ccgtttcgga ttataaaccg cctcccgcgc cggaaaacag
     4561 caaaatgccg tctgaaggct tgggcttgct caaaaaaggg agggatttcc ccgcctgacc
     4621 gggatgggcg ttcagacggc attgcctgcc gccggtttat agtttttgca aatcaatatt
     4681 aacaagctga aaaaaaacaa tatacccgcc cggtcttaat gttaacggag tatggaaatg
     4741 aaacaaatgc ttttggccgt cggcgtggcg gcggtgttgg cgggctgcgg caaggatgcc
     4801 ggcggttacg agggttattg gcgcgaaaag tcggacaaaa aagagggcgt gattgccgtc
     4861 aaaaaagaaa aaggcaatta cttccttaat aaaatcaacg tgtttacagg caaggaggag
     4921 tctttgcttt tgtctgaaaa agacggcgcg ctttcgataa acacggggat aggggaaatc
     4981 ccgatcaaac tttccgacga cgggaaagag ctgtatgtcg aacgcaggcg gtatgtgaaa
     5041 accgatgcgg cgatgaagga caaaatcatc gcccaccaga aaaagtgcgg acaaacggca
     5101 caggcatacc tcgacgcgcg aaatgcgttg ccgtcaaacc aaacgtatca gcagcgtcag
     5161 gcggcgatcg agcaattgaa acggcggttt gaagccgagt ttgacgaatt ggaaaaagaa
     5221 atcaaatgca acggcagaac cccggcattg ttgctttggc aggggacaac cgggaggatg
     5281 ccgccgtccg aatcggacgc gtggtttcca taccggtacg ggcgggcggg aatgtccgcc
     5341 tttttttgtt cggatgcgtt tgaatacccg tccgattctg tccgtttgca agggggattt
     5401 ccgttcgggc ggaaatccat tccgtcaaat aagaaaggcg ttttgtccgg ccggccggct
     5461 tgtgcctgcg gtatcgcaaa acttcgcctt aatgcgcccg attggctagg gacgggcttc
     5521 ggacggcatt gttttgccgg ttcacgggcg gtattcgggc ttcatgccgt tgggcaggag
     5581 ctgccagacg tatcccgtgg ttttctgttt gccggcaagt tcgccggctt cgtcgccgta
     5641 accccaaaaa taatccacgc gcaccgcgcc tttgatcgcg ctgcctgtat cctgcgccat
     5701 aatcaggcgg ttgaggactt tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc
     5761 gcccaaggta atgtagtgcc ggtcgattgc gccggcgtat tcccccatca gcggcgtgcc
     5821 cagtgcgccg acggggccct cattgccgct tccggcaagc tcgcggaaaa agatatagct
     5881 ggggttttgc cccaaaactt cggcgaggcg ttgcggattt tgccgcatat aggctttgat
     5941 gccctgcatg gaggtttgcc cgagcttgag gtagcctttg tccgccatat agcgtccgat
     6001 ggaaacatac ggatgctcgt ttttgtcggc atagccgatg cggatgtatt tgccggacgg
     6061 ggttttcaga cggcccgagc cttgtatgtg catgaaaaaa agttcgacgg ggtcttcggc
     6121 gtaaccgagt atcggggctt tgccgtcaag cgcgccgccg ttgatttggt tgcgcgtgtg
     6181 gtaggggagg aagcggcttc cttcaaacct gcccttgatt gccgttgtgc gcgcggtaat
     6241 ggggaatcgg gaaaggtcgg cggtatgcgt gccgccggca ttgtcgattg tgccgctgtt
     6301 ttttcccgtc tgcctgatgc ggacaagggc tttcccgccc cgcaaaccgg caggcagggg
     6361 gacggagata aaatcgtcgg gaatgccgta aatcgggaag cgggcccgtt ccgtccgcct
     6421 gtcgtcgccc ttcagcaccg gctcgtaata gccggtaacc gtacctgcaa ggcttccgtt
     6481 gcctgcaacc tgccacggcg tgaaatagcg ttcaaaaaac cgctttgcct gaaaggaatg
     6541 cacgggggtt tgaaaggctt gggcgcacac atcctgccag ccttggcggt ttttcaaatt
     6601 ggcgcagccg aggcggaagg attgcaggct tttggcaaaa tcctgcgccg cccagtgggg
     6661 catggacagg tgcggcacaa cggtatagac ggccccgccg ccggcaaccg tcgttccggc
     6721 ggggtcgggg atgccggccg gccggtccgg gccgttgatg acggatgtgt cgggttgcgg
     6781 aaaggtttgg atgctcctgc tttggcaggc ggcgaggatg gcggcggcga tgccgtacag
     6841 ggcggagcgg agcaggtgtt ttttcataat ggacaatgtt gtcgtcagta ataaaaaaag
     6901 atggtttcgg gcggcgttgc ggtagccggg gagaggggat tttaacacag ggcgcagccg
     6961 cagcctgcgg aactttccgc cacgcggcaa tgcagataaa aataacttgc atttgtattt
     7021 gcaagtaatg aaaatattcc gataatatat tattaattat ccttgttcat tcgcgtttac
     7081 gctggtcgct tttttaatta tgttgcgcga gggtatcgaa gccgcgctca tcgtcggcat
     7141 cgttgccggt tttttgaaac agtccggaca ttcaaaaact gatgcctaag gtctggttcg
     7201 gggtcgtcct tgcttctttg atgtgtttgg gcatcggata cggcatccat tcggcaacgg
     7261 gcgagattcc ccggaagcag caggagttcg tcgtcggcat tatcggtttg gttgccgtcg
     7321 cgatgctgac ttgtgtggtt ttatggatga aaaaggcggc gcgttcgatg aagcggcagc
     7381 ttcgggattc tgtacaggcg gctttgaacc gtggcagcgg tcaaggatgg gccttggtcg
     7441 gtatggcgtt tcttgccgtg gcgcgcgaag gtttggagag cgtttttttc ctgcttgccg
     7501 tattcaaaca gagcccgacg tggcagatgc cggccggcgc ggtagcgggg gttttggctg
     7561 ccgccgtgat tggcgcgttg atttatcagg gcgggatgcg cctgaatctg gcgaagtttt
     7621 tccgttggac gggggcgttt ctgattatcg ttgccgccgg tctgcttgcc ggctcgctgc
     7681 gtgcgctgca tgaggcaggc atttggaacg cgcttcagga cattgtgttg gacccatcaa
     7741 aatatttgca cgaagacagt ccgttgggtg tgctgctcgg cggatttttc ggctataccg
     7801 accatccggc gcagggcggg gccttggttt ggctgctgta ccttattccc gtcatgattt
     7861 ggtttttgcg cggcagcagg ccgtctgaaa ctttaacccg taaagaggaa ttgaaatgag
     7921 aaaattcaat ttgaccgcat tgtccgtgat gcttgccttg ggtttgaccg cgtgccagcc
     7981 gccggaggcg gagaaagccg cgccggccgc gtccggtgag acccaatccg ccaacgaagg
     8041 cggttcggtc ggtatcgccg tcaacgacaa tgcctgcgaa ccgatgaatc tgaccgtgcc
     8101 gagcggacag gttgtgttca atattaaaaa caacagcggc cgcaagctcg aatgggaaat
     8161 cctgaagggc gtgatggtgg tggacgaacg cgaaaatatc gccccggggc tttccgacaa
     8221 aatgaccgta accctgctgc cgggcgaata cgaaatgacc tgcggccttt tgaccaatcc
     8281 gcgcggcaag ctggtggtag ccgacagcgg ctttaaagac accgccaacg aagcggattt
     8341 ggaaaaactg ccccaaccgc tcgccgacta taaagcctac gttcaaggcg aggttaaaga
     8401 gctggcggcg aaaaccaaaa cctttaccga agccgtcaaa gcaggcgaca ttgaaaaggc
     8461 gaaatccctg tttgccgcca cccgcgtcca ttacgaacgc atcgaaccga ttgccgagct
     8521 tttcagcgaa ctcgaccccg tcatcgatgc gcgtgaagac gacttcaaag acggcgcgaa
     8581 agatgccgga tttaccggct tccaccgtat cgaacacgcc ctttgggtgg aaaaagacgt
     8641 atccggcgtg aaggaaaccg cggccaaact gatgaccgat gtcgaagccc tgcaaaaaga
     8701 aatcgacgca ttggcgttcc ctccgggcaa agtggtcggc ggcgcgtccg aactgattga
     8761 agaagcggcg ggcagtaaaa tcagcggcga agaagaccgt tacagccaca ccgatttgag
     8821 cgacttccaa gctaatgcgg acggatctaa aaaaatcgtc gatttgttcc gtccgttgat
     8881 tgaggccaaa aacaaagcct tgttggaaaa aaccgatacc aacttcaaac aggtcaacga
     8941 aattctggcg aaataccgca ccaaagacgg ttttgaaacc tacgacaagc tgagcgaagc
     9001 cgaccgcaaa gcattacagg ctcctattaa cgcgcttgcc gaagaccttg cccaacttcg
     9061 cggcatactc ggcttgaaat aagccgcaag cgttcagacg gcatttggcg gcagataccg
     9121 tctga
//
LOCUS       DAXDSD010000085         9031 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXDSD010000085 DAXDSD010000000
VERSION     DAXDSD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.085"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            104..1060
                     /locus_tag="ACFB2Q_001657"
     CDS             104..1060
                     /locus_tag="ACFB2Q_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC0918642.1"
                     /translation="MSQNQPAQPTKRNLFKTALAVGAIGAIGGYFGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPRQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPSAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRD
                     FPNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQANLADGFI
                     FVQNLLNGEPLEECISPFGGGYFFVLPGVGKGGFLGQGLPGV"
     gene            complement(1132..1461)
                     /locus_tag="ACFB2Q_001658"
     CDS             complement(1132..1461)
                     /locus_tag="ACFB2Q_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC0918643.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(1537..2907)
                     /gene="glmU"
                     /locus_tag="ACFB2Q_001659"
     CDS             complement(1537..2907)
                     /gene="glmU"
                     /locus_tag="ACFB2Q_001659"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC0918644.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPTGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(2962..3234)
                     /locus_tag="ACFB2Q_001660"
     CDS             complement(2962..3234)
                     /locus_tag="ACFB2Q_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC0918645.1"
                     /translation="MRKTFLILTVSAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(3227..3940)
                     /locus_tag="ACFB2Q_001661"
     CDS             complement(3227..3940)
                     /locus_tag="ACFB2Q_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC0918646.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHTPPFIDASVSDMAQLARYAETLSER
                     RQNHYNTRTNPENTKECYA"
     gene            3999..5000
                     /locus_tag="ACFB2Q_001662"
     CDS             3999..5000
                     /locus_tag="ACFB2Q_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC0918647.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSLEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5094..5942
                     /locus_tag="ACFB2Q_001663"
     CDS             5094..5942
                     /locus_tag="ACFB2Q_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC0918648.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            5968..6450
                     /locus_tag="ACFB2Q_001664"
     CDS             5968..6450
                     /locus_tag="ACFB2Q_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC0918649.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDAV
                     "
     gene            complement(6534..8102)
                     /gene="msrAB"
                     /locus_tag="ACFB2Q_001665"
     CDS             complement(6534..8102)
                     /gene="msrAB"
                     /locus_tag="ACFB2Q_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC0918650.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGEFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGKVK"
     gene            8248..>9031
                     /locus_tag="ACFB2Q_001666"
     CDS             8248..>9031
                     /locus_tag="ACFB2Q_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HFC0918651.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRAESAVETVSGAVEQVKETVAEMPSEAGEAAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAR"
BASE COUNT         1849 a         2327 c         2760 g         2095 t
ORIGIN      
        1 cgccatatgt tgcagggcgg gctttcagac cgccgctatc ggttaatgga aaaacggcgt
       61 gcagggatgc ccaccctgct gcacggatat tgaaggaaac gccatgagtc aaaaccaacc
      121 cgcacaaccg accaaacgca atctgttcaa aaccgccctt gccgtcggcg caatcggcgc
      181 aatcggaggt tatttcggcg gcaaaaaaca gggcgaaacc gccgaacgca ccgccgaaag
      241 ccaacactcg ccccaagcct atccctgcta cggcgaacat caggcaggta tcgttacgcc
      301 gcggcaggcg ttttccatta tgtgcgcctt cgacgtaacc gcgcaaagtg ccaagcagct
      361 ggaaaacctg ttccgcacac tgaccgcccg catcgagttt ctcacccaag gcggagaata
      421 ccaagacggc gacgacaaac tcccgtcagc cggcagcggc attttgggca aagccttcaa
      481 ccccgacgga ttgaccgtta ccgtgggggt gggcagcagc ctgtttgacg gccggttcgg
      541 actcaaagac aaaaaaacgg ttcatttgca ggaaatgcgc gacttcccca acgataagct
      601 gcaaaaaagc tggtgcgacg gcgatttgag cctgcaaatc tgcgccttca cccccgaaac
      661 ctgccaagcc gccctgcgcg acatcatcaa acacaccgcc caaaccgccg tcatccgctg
      721 gagtatcgac gggtggcagc ctaaatccga acccggcgcg atggcggcgc gcaacctgtt
      781 gggcttccga gacggcacgg gcaaccccaa ggtttccgat cccaaaaccg ccgacgaggt
      841 tttatggacg ggcgtggccg ccaacagcct cgacgaaccg gagtgggcga aaaacggcag
      901 ctatcaggca aatcttgccg acggtttcat cttcgtgcaa aacctcctca acggcgaacc
      961 gctggaagaa tgcatcagcc ccttcggcgg cggctatttc ttcgtcttgc ccggcgtggg
     1021 aaaaggcgga ttcttgggac aagggctgcc gggcgtataa acctgcctga cggacaaatg
     1081 ccgtccgaac cttgccaaac gggttcggac gggattttac tgtttggacg gtcaggcttt
     1141 tttgacgaat tcggatttta aattcatcgc gctgccgtcg attttgcagc cgatgttgtg
     1201 atcgccttcc tgcaggcgta tgcctttgac cttcgtacct tgtttgatcg tcatcgagct
     1261 gccttttacc ttgaggtctt tgatgaggat gacggtgtcg ccgttttgca gcgccgcgcc
     1321 gttggcatcg cgcacttgag ccgcaaggtc ggcggcggat tcggtttcat tccattcgtg
     1381 ggcgcattcg gggcagatgt attgtccgcc gtcttcatag gtgtattcgg aggcgcattg
     1441 cgggcaaggg ggtaatgaca tggtttgccg tccttatcgg atgtttgttt tgggatgccg
     1501 tctgaaacct gaaaccggct tcagacggca tggcttttat tgtttgtctt tttccggcct
     1561 tacccagcct tcgatgacgg tttggcgggc gcgggcgagg gcgagtttgt tgtcttcgat
     1621 attgcgggta atcgcgctgc ctgcgcctgt ggttactttg ttgcccagtg ttacaggggc
     1681 aaccagtacg cagttcgaac cgatgcgcac ttcgtcgccg atgacggttt tgtgtttgtg
     1741 cacgccgtcg tagttggcaa taatcgtgcc cgcgccgaag ttggttttgc tgccgacttc
     1801 ggcgtcgcct atgtaggtga ggtggttggc tttggtgcct ttgccgatgg cggcgttttt
     1861 gatttcgacg aagttgccga cgtgtacatt gtcagccagt ttggcttgcg gacgcaggcg
     1921 ggcgtacggg ccgattcggt tgttttcgcc gacttcgcag ccttcgaggt gggagaaggg
     1981 ggcgattttg ctgtttgcgc cgattttggc gtttttgatg acgcagtttg cgccgatttc
     2041 gacgttgtcg ccgagttcga cttcaccttc gatgacgacg ttgacgtcaa tcacgacatc
     2101 ctgtccgtgt ttcagacggc cccgcaaatc gaaacgcgcc ggatcgcgca gggtcacgcc
     2161 tgctttgagc aattcttgcg cctgttcggt ttggaagatg cgttcgagtt cggcgagctg
     2221 gcgtttattg ttcacgccgg cggcgaggtg ggaggcgcgc actcggacgg ggcggacttt
     2281 gatgccgtcg gcaacggctt tggcgatgag gtcggttaaa taatattcgc cttgggcatt
     2341 gttgctggaa aggctgttca gccagttttc cagtttggcg ttgggcagga cgaggatgcc
     2401 tgtgttgatt tcgcggatgg ttttttgggt ggcacttgcg tctttttctt cgacaatggc
     2461 ggttacgctg ccgctgccgt cgcggatgat gcgccccaag cctgtcgggt cggcgggcac
     2521 gtcggtcaac agcccgactt cgttgcctgc ggcttcgagc agggtttcga gggtttcaac
     2581 gtcaattaaa ggaacgtcgc cgtacaacac cagcgtgcgg ccttcggagg caaggtgggg
     2641 cagggcggtt ttgacggcgt ggccggtgcc gagttgttcg gtttgttcga cccagacggc
     2701 atcgcgtttg acagtgtcta aaacttgctc tttgccgtgt ccgacgacga cgcagatgtt
     2761 ttgcggattc agcgcggcgg cggtgtcgat aacgcgctcg accatgggct tgccgccgat
     2821 gcagtgcagc actttgggca tttgggaata catgcgcgtg cctttgccgg cggcgaggat
     2881 gacggtgttt aaagtgtttt gcggcatagc gttttcctgt gccatgccgt ctgaagcggt
     2941 ttcagacggc atagggtagg tttatcggtt ttgaaacttt ggtttttgcc agtgttggcg
     3001 atgctcttcg tcggcgttgt tgccggtttg gtcgggcagc acggcgcggc gttcgggacg
     3061 gtattggttg tagttcatat ttttcgagta gctgccgtct tggtaataaa cgggcgtgcc
     3121 ggcgggatat ttttgacgga cggcggtttt gccgttgccg tcttgataag tttcccacgc
     3181 gcagcccgac aaaagggcgg cggagacggt caggataagg aaggttttac gcataacatt
     3241 ctttcgtatt ttcggggttt gtgcgggtat tgtaatgatt ttggcggcgt tctgacaaag
     3301 tttctgcata ccgtgccagt tgcgccatat cgcttacgga ggcatcgata aagggcggcg
     3361 tgtgggattt tgcaccgaac cggacggttt tcatgcccaa tgcttttgcc tgatggaggt
     3421 tgtccgcgct gtcgtccacc ataatgcagc attcgggcgg tacgtccaac aggcggcaga
     3481 cattgagata cgcttgcgga ttgggtttgt acagcagtcc gaaatcatcc gtgccgaaca
     3541 gtgcgtcgaa acagttttcc aaaccgagtg cgccggcaac ggcacggacg taaaacgacg
     3601 ggccgttgga aaaaaccgcc ttgcgcccgt tcagacggca tagggtgttt tccgtatccg
     3661 ccatgccgtg cagcctggtc aggattgcat cgatcggatg gctttcgcgt aaaaattccg
     3721 caatgtcgat ttcgggatgg tggatttgca gtccggcgag cgttgcgccg taacggtgcc
     3781 aatagtcttg acgcaggtcg gacgcggcgg attcggagag tttgaggcgg cgtgccatat
     3841 agcgcgtcat agcgcggttg atgaggtgga agatgcctgc gtcggcatcg tgcagcgtgt
     3901 tgtcgaggtc gaacagccac acggtcgggt tttcttgcat tttgaaccgt gaaaatttgt
     3961 tagaatgtta ttttacagcg aataaaggaa gactcggaat gaaacggaaa atttggctgc
     4021 tgccgctgct ggcggtttcg gcatacctgc aggcgcagac ggaagtcagg ctggcggtgc
     4081 ataagtcgtt cagcctgccc aaagggctga ttgcgcgctt cgagcgggca aacgatgcga
     4141 aagtgtcgat tattcaggcg ggcggcgcga acgaaatgct caacaaactg attttgagcc
     4201 gagccaaccc gattgccgat gcggtgtacg gtttggacaa cgccaatatc ggcaaggcgc
     4261 gggaaatggg cattttggcg gcggcgcaac ccgaatccgc ccccgtcgcg gtcgggctgc
     4321 cttcggcttt ggcggtcgat tacggctatg tgtccgtcaa ttacgacaaa aaatggtttg
     4381 aaggcaaaaa gctgcccctg ccgcaaaccc tgcaggattt gacccgcccc gaatataaaa
     4441 acctattggt cgtgccgtcc cccgccacat cttcccccgg cttgggcttc ctgatggcga
     4501 acatcggcgg tctgggcgaa gaaggcgcgt ttaaatggtg ggcacagatg cggcagaacg
     4561 gcgtgaaggt cgccaaaggt tggagcgagg cgtattacac cgacttttcg cacaacggcg
     4621 gcgcgtatcc gctggtggtc ggttatgccg ccagccctgc ggcggaagta tatttttcca
     4681 aaggcaaata cagcgagccg ccgacgggca acctgttttt aaaaggcggc gtattccgtc
     4741 aggtcgaagg cgcggcggtc ttgaagggcg cgaaacagcc gaagctggcg gcaaaactgg
     4801 tgcaatggct gcaaagtctg gaagtgcagc aggcggttcc gtctgaaatg tgggtttacc
     4861 ccgccgtcaa aaacacgcgc ctgcccgacg tgttccgctt cgcccaagcc ccggcgcaca
     4921 ctaccgcccc cgcgcagcgc gatattgatg cgaaccagcg cggatgggtt tcccgttgga
     4981 tcaggacggt tttgaaataa aacaaacata ccgccccgca gagcttcaga cggcattttt
     5041 acacctgtgc cgattacgcc gcacggggcg gatgttcgat cgagaggaaa acaatggact
     5101 tcaaacaatt tgatttttta cacctgatca gtgtttccgg ttgggggcat ctggctgaaa
     5161 aggcgtgggc gttcgggctg aaccttgccg ccgcgctgct tattttcttg gtcgggaaat
     5221 gggcggcgaa acgcattgtc gccgtgatga gggcggcgat gacgcgcgcg caggtcgatg
     5281 ccacgctgat tagttttttg tgtaatgttg ccaatatcgg cttattgatt ttggtgatta
     5341 ttgccgcatt gggcagattg ggcgtttcca caacatccgt aaccgcctta atcggcggcg
     5401 cgggtttggc ggtggcgttg tccttaaaag accagctgtc caattttgcc gccggcgcgc
     5461 tgattatcct gttccgcccg ttcaaagtcg gcgactttat ccgtgtcggc ggttttgaag
     5521 gatatgtccg ggaaatcaaa atggtgcaga cttctttgcg gacgaccgac aacgaagaag
     5581 tcgtgctgcc caacagcgtg gtgatgggca acagcatcgt caaccgttcc agcctgccgc
     5641 tttgccgcgc ccaagtgata gtcggcgtcg attacaactg cgatttgaaa gtggcgaaag
     5701 aggcggtgtt gaaagccgcc gccgaacacc ccttgagcgt tcaaaacgaa gagcggcagc
     5761 ccgccgccta catcaccgcc ttgggcgaca atgccatcga aatcacatta tgggcttggg
     5821 caaacgaagc agaccgctgg acgctgcaat gcgacttgaa cgaacaagtg gtcgaaaacc
     5881 tccgcaaagt caatatcaac atcccgttcc cgcaacgcga catacacatc atcaattctt
     5941 aaacgccgtc tgaaaggggg gtgggaaatg gacgcgttgc acaccatcgc ccgaaacctg
     6001 acgaaaaaac gtcaaaccgt aagctgtgcc gaatcctgca cgggcggaat gcttgccgcc
     6061 gcattcacaa gcgttgcagg cagttcgcaa tggttcgacc agagttttgt aacatacagc
     6121 aacaaagcca aagaataccg cttgggcgtg ttgcccgaaa ccctgctcga acacggcgcg
     6181 gtcagccgcc aaaccgtcta tgagatggcg cgcggcgcga aagccgtggc tcaggcggat
     6241 tacgccgtcg gtatttccgg catcgccggc ccgggcggcg gcgaaagcaa acccgtcggc
     6301 acggtttggt tcgggtttgc ctttccgggc ggaagttgcg aagcaatgcg ccgttttgac
     6361 ggcaaccgcg aatccgtccg cgcgcaggcg gtcgccttcg cgttggaacg gttggccggg
     6421 ctggttgaaa acggcggcga tgctgtctga acaaatcccc gccggataaa aaatgccgtc
     6481 tgaaacgttg caggtttcag acggcatttt gccgggttag gcggcggtgc ggcttatttc
     6541 actttgccct tcaacgcgcc gtagcctgcc gcgtccattt gttccagcgg gatgaatttc
     6601 aagctcgcgc cgttgatgca gtagcgcagt ccgcctttgt cgcgggggcc gtcggggaag
     6661 acgtgtccca agtgcgaatc ggcggcgcgg cttctgactt cggtgcggcg catattgaag
     6721 ctgaaatcat cgtgttcggt aacggatttt gcatcaatcg ggcgcgtgaa gctcggccag
     6781 ccgcagccgg aatcatattt gtcggcggag ctgaacaggg gttcgccgct gacaacgtcc
     6841 acataaatgc cgggtttgaa caaatggtcg tattcgtggc tgaaggcgta ttcggtcgcg
     6901 ctgttttggg tcacttggta ttgctcttcg gtcagggtgc gtttgagttc ggcgtcactc
     6961 ggttttttat acgttgccgc gtcgaagcct ttgccttgcg gtgcggcttt ggttttgccc
     7021 ggcagcggtt cgtcggcttt gcggatgtcg atgtggcagt agccgttggg gtttttaatc
     7081 aggtagtcct gatggtattc ctcggcgtcg tagaagtttt tcagcggttc gttttcaaca
     7141 acgaggggca gttggtattt ttgctgctcg cgtttgaggg cggcggcgat gacggctttt
     7201 tcggcggggt cggtgtagta cacgccgctg cggtattgcg tgccggtgtc gttaccctgt
     7261 ttgttgaggc tggtcggatc aacgacgcgg aaataatatt gcaggatgtc gtccaggctg
     7321 agtttgtcgg catcgtaggt cactttgacg gtctcggcat ggcccgtatg gcggtaggac
     7381 acgtcttcgt agctcgggtt ttccgtgttg ccgttggcgt agccggatac cgcgtcaacc
     7441 acgccgtcga tgcgttggaa ataggcttcc aagccccaga agcagccgcc ggcgaggtag
     7501 atggtgcgcg tgttcatgat tgctgaatcc tttttctgag tgtcgggttt gtagaacgaa
     7561 tgtttcaaac tgcccaaatc ggcattcggg ttgcggatta acgccaatgc ctgcgcttcg
     7621 ttgatgctgc ctttgacgat gcgctgcacg tcgccgtctt taccgattaa cgcccaagaa
     7681 ggataaacgc tgatattcag gttttgggcg atcgtgccgc cgttgtcggt aacgacgggc
     7741 agcttggggt agttcaaacc ggcataccat ttttgaaact cgccgtcttt tttctcgtgc
     7801 aaaaagccgg gggaggcgac ggtaatcagg ttggcggagc tgaattttgc atcttgcgcc
     7861 catttctcgg cctgtcccaa ttcggacaaa cataaaggac accagctcgc ccaaaattta
     7921 atcagcgtcg gtttgtcttt tttcaaataa acactggcgg ggcggttgtc cgcggttttt
     7981 aacgtggata aagtgtgcgg cacggtcgcg gccccggcat cgacgatttt gggcgaacaa
     8041 gcgcccagcg caagcaggca gccgaacttg gcgcaaaggg aaaagaaagt acggtgtttc
     8101 attttgatgt ttcctgtgtg gacggtttgc atgattagac gtttgagatg ccgaaacctt
     8161 acggcccgga ttttcagaca accttgccgc gcaaaatacg ctacaatacg ccctatttca
     8221 agtttctaaa attaaaagga aaattcaatg ttcagcttct tccgtcgcaa gaaaaaacag
     8281 gaaacgccgg ctctcgagga ggcccaagtt caggaaaccg cagcaaaagt agaatctgaa
     8341 gttgctcaaa tagttggaaa tattaaagaa gatgtcgaat ctttagcaga aagcgtcaaa
     8401 gggcgggccg aatctgccgt tgaaaccgtc agcggtgcgg ttgaacaggt aaaggaaacc
     8461 gttgccgaga tgccgtctga agcaggggaa gcggcggaac gcgttgaatc cgcaaaagaa
     8521 gctgttgccg aaaccgtcgg cgaggctgtc gggcaagttc aagaagccgt tgcgacaact
     8581 gaagaacaca agctcggttg ggctgcgcgt ttgaaacaag gcttggccaa atcacgcgac
     8641 aaaatggcga aatcgctggc cggcgtgttc ggcggcggac aaatcggcga ggatttgtac
     8701 gaagagctgg aaaccgtgct gattaccggc gatatgggca tggaggccac cgaatacctg
     8761 atgaaagacg tgcgcgaccg cgtcagcctc aaagggctga aagacggcaa cgaattgcgc
     8821 ggcgcgttga aagaagcctt gtacgacctg attaagccgt tggaaaaacc gctggtcttg
     8881 cccgaaacta aagagccttt cgtgattatg cttgccggta tcaacggcgc gggcaaaacc
     8941 acgtctatcg gcaaactcgc caaatatttc caagcgcagg gcaaatccgt attgctggcg
     9001 gcaggcgata ctttccgcgc cgccgcgcgc g
//
LOCUS       DAXDSD010000086         8697 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXDSD010000086 DAXDSD010000000
VERSION     DAXDSD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.086"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..258)
                     /locus_tag="ACFB2Q_001667"
     CDS             complement(<1..258)
                     /locus_tag="ACFB2Q_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918652.1"
                     /translation="MNTKTELQKLLEEDISTLKETLIRVDALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYS"
     gene            complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFB2Q_001668"
     CDS             complement(320..2944)
                     /gene="alaS"
                     /locus_tag="ACFB2Q_001668"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC0918653.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPEMLASAEGWLCQKLS"
     gene            3148..4278
                     /locus_tag="ACFB2Q_001669"
     CDS             3148..4278
                     /locus_tag="ACFB2Q_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC0918654.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4319..5221)
                     /locus_tag="ACFB2Q_001670"
     CDS             complement(4319..5221)
                     /locus_tag="ACFB2Q_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC0918655.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5360..5857
                     /locus_tag="ACFB2Q_001671"
     CDS             5360..5857
                     /locus_tag="ACFB2Q_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HFC0918656.1"
                     /translation="MAGMKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAP
                     AKGFRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDS
                     MYEIYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMK
                     MQPLK"
     gene            complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFB2Q_001672"
     CDS             complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="ACFB2Q_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC0918657.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            6953..7288
                     /locus_tag="ACFB2Q_001673"
     CDS             6953..7288
                     /locus_tag="ACFB2Q_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC0918658.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            7419..7889
                     /locus_tag="ACFB2Q_001674"
     CDS             7419..7889
                     /locus_tag="ACFB2Q_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918659.1"
                     /translation="MKYHRLALFAAISCLLLSAVFIAPYLTAFHEQEKIFEYADLTVT
                     APNRSGRAIKLEADGRQYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNG
                     VLLEYRNSGSVYSNKDFSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            7995..8558
                     /gene="pgsA"
                     /locus_tag="ACFB2Q_001675"
     CDS             7995..8558
                     /gene="pgsA"
                     /locus_tag="ACFB2Q_001675"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC0918660.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVAAVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
BASE COUNT         2049 a         2147 c         2329 g         2172 t
ORIGIN      
        1 actataaaaa ttaactttat tctctaaggt tgacaatttt tcaaatacca aacttgtatc
       61 aaggatgggc aattctatag ttaaacctat ttcttttgcg agaatattta attgtttgtc
      121 aattaaccat cgacggacaa taggagttgc aatgctacgt acatatcttg gaggaagagc
      181 atctacacga attagtgttt cttttaaagt tgatatatcc tcttctaaaa gtttttgtaa
      241 ctctgtttta gtattcattc taacaatcca aaatataagc catttgaaca agactcaaat
      301 ggcttattaa gatttaaatt taacttaatt tctgacaaag ccaaccttcc gcgcttgcca
      361 gcatttcggg caatttgtcg gcatccgtac cgccggcttg cgccaagtcg ggacggccgc
      421 cgcctttgcc gccgacttgt tcggctgcaa atttaaccag atctcctgct ttgacttttc
      481 cggtcagggc tttggatacg ccggcgcaca gggagacttt accctcgttt actgccgcta
      541 aaagaatcac ggcgttgtcg gattttccgg tcaggtcggt tacggtttcg cgcagggcgg
      601 ctgcgtcggc ttcgatttgt gcggcaacga gtttggccgc gcccaggtct tttgcgtcgt
      661 ccaagagttt ggcgcctgcg tggacggcga gttcggcttt ggcgcgtgcc aattcttttt
      721 ccaatgcttt ggcgtgtgcc gcgcctgctt ggattttcgc cagtacgtct ttttcggttt
      781 gggctttggt ttcggcaatg atgtctttta ccaaacgctc ttgctcttgc gcccatttga
      841 gtgcgttcag gccggtgatg gcttcgatac ggcgtacgcc tgcggcaata ccgccttcgc
      901 tgatgatttt gaagaggccg atgtcgccgg tgcgtgaaac gtgtgtgccg ccgcacaatt
      961 cggtagagaa accgcccatt tgcagtacgc gtacttcgtc gccgtatttt tcgccgaaga
     1021 gcatcatggc gtcggttttt tgcgcgtctt ccatgctcat aatggctgcg ttgacggcaa
     1081 cgttggctaa aacggcttcg ttgacgcggc gttcgacttc agcgatttct tcggcggtta
     1141 ccgcttgggg atgggaaatg tcgaaacggg tggattcggc ggtaaccaaa gagccttttt
     1201 gttcgacgtg tccgcccaat acgtcgcgca gggctttgtg catcaggtgg gtcgcgctgt
     1261 ggttgcgcat attggcattg cggatttcgt catccacttt ggcggtaacg ctgtcgccga
     1321 ctttcagacg gcctgaagtt tgtacgccga attgtccgaa tacggccgct ttgatttttt
     1381 gggtatcgcg tacttcaaag cggttttcgc ctgcgaagat atagccgacg tcgccgactt
     1441 ggccgccgga ttctgcatag aacggggtaa agtcgataac gacggcaccg ctgtcgcctt
     1501 cgttcaattc gacgacttgc ccgccgtctt tgtagagggc gaggactttg gattcggttt
     1561 ggcgttcgct ataaccttta aactcggtgt cttgaccgtc gtaaggcagt tgggcgttgg
     1621 ctttgaagct ttgggcggcg cgtgcgcgtg cgcgttgggc ttccatttcg cgctcgaagc
     1681 ctgcttcgtc cggttcgata ttgcgttcgc ggcagatgtc ggcagtcagg tcgtatggga
     1741 agccgtaggt atcgtagagt ttgaagataa tttcgccgcc gagtgttttg ccgcctttgg
     1801 ccagcgcgtt ttccaacaaa gccataccgg tttccagagt ttgggcaaaa cggctttctt
     1861 cgtttttcaa agcttcttcg atttgcgcct gtttttcttt caattcagga tacgcgccgc
     1921 ccatctcttg aaccaaatcg gcaacgagtt tgtggaagaa cggtttgctt tgacccagtt
     1981 tgtaaccgtg gcgcacggcg cggcggatga tgcggcgcaa tacgtagccg cgtccttcgt
     2041 tggaaggcaa tacgccgtct gcaatcagga atgagcagga gcggatgtgg tcggcgataa
     2101 ctttcaggct gggttcttcc atactgaacg gcgcgccggt ttcgcgggca acggctttga
     2161 gcaggtcttg gaacaggtcg atttcgtagt tgctgtggac gtgctgcatc acggcggcca
     2221 tacgttccaa gcccataccg gtatcgacgg acggcttggg cagtggattc atattgcctt
     2281 gttcgtcgcg gttgaactgc atgaatacgc agttccaaat ttcaatccag cggtcgccgt
     2341 cttcttcggg gctgccggga atgccgcccc agatttcttt gccgtggtcg tagaaaattt
     2401 cggagcaggg gccgcaaggg ccggtgtcgc ccatttgcca gaagttgtcg gacgcgtatt
     2461 tcgcgccttt gttgtcgccg atgcggacga tgcgttcaga cggcataccg atttcgttca
     2521 accagatgtt gtaggcttcg tcgtcttctg cgtaaacggt cgccaagagt ttgtctttgg
     2581 ggatgttgag ccattcgggg gaagtgagaa actcccaagc gaagtggatc gcgtcgcgtt
     2641 tgaagtaatc gccgaaggag aagttgccca tcatttcaaa gaaggtgtgg tggcgggcgg
     2701 tgtagccgac gttttccaag tcgttgtgct tgccgcctgc gcgtacacat ttttgcgcgg
     2761 tggtggcgcg gctgtacggg cgtttgtcga aacctaaaaa tacgtcttta aactggttca
     2821 tgcctgcgtt ggtaaacagc agggtcgggt cgtcgtgcgg cacgaggctg gaagagcgga
     2881 cgacggtgtg gcctttggtt tcaaaaaatt ttaggaattt ttggcgcagt tcggaggttt
     2941 tcataatttt ttcaatgtct ctcaaatgtc ttgtcatggt aaaagcaggg aaaacgaacg
     3001 gcggtatatt accgcaaatc cctgtttcta gctatggaag cggcggcttt cagacggcat
     3061 tgcgggattt tgaatgccgt ctgaagccct gttaccaata tcggctataa tggccgcttt
     3121 ctccaacccg atatgcaagg aatgataatg gtcaaacatc tgccactcgc cgtcctgact
     3181 gctttgctgc ttgcagcgtg cggcggttcg gacaaaccgc ctgccgaaaa accggcaccg
     3241 gcggaaaacc aaaacgtatt gaaaatttat aactggtcgg aatacgtcga tccggaaacc
     3301 gttgccgatt ttgaaaagaa aaacggcatc aaggttactt atgatgtgta cgacagtgat
     3361 gaaacgctgg aaagcaaggt gctgaccgga aaatccggtt acgacattgt cgcgccgtcc
     3421 aatgcgtttg tgggcaggca gattaaggca ggtgcgtatc agaaaatcga taagtcgatg
     3481 attcccaatt ataaacatct caaccctgaa atgatgaggc tgatggacgg ggtcgatccc
     3541 gaccacgaat acgccgtgcc gttttattgg gggacaaata ccttcgccat caataccgaa
     3601 cgcgtgaaaa aggctttggg tacggacaag ctgccggaca accagtggga tttggtgttc
     3661 aaccccgaat acacgttcaa actcaaacaa tgcggcatca gctatttgga cagcgcggcg
     3721 gaaatttatc ccatggtgtt gaactatttg ggtaaaaacc cgaacagcag caatacggaa
     3781 gacatcaggg aggcaaccgc cctgctcaag aaaaaccgcc ccaatatcaa acgctttact
     3841 tcgtccggct ttatcgatga tttggcgcgc ggcgatacct gcgtaacaat cggtttcggc
     3901 ggagatttga acatcgccaa acgccgtgcc gaagaagcgg gcggcaagga aaaaatccgc
     3961 gtgatgatgc cgaaagaggg cgtggggatt tgggtggatt ctttcgtgat tccgaaagat
     4021 gcgaaaaacg tcgccaacgc gcacaaatac atcaacgact tcctcgatcc ggaagtgtcg
     4081 gcgaaaaacg gcaatttcgt tacctacgcg ccttcgagca agccggcgcg cgatttgatg
     4141 gaggacgaat ttaaaaacga caatacgatt ttcccgagcg gggaagattt gaaaaacagc
     4201 tttatcatgg tgcctatccg gccggcggca ttgaagttta tggtgcgcca gtggcaggat
     4261 gtgaaggcgg ggaaataaag cccgatatgc cgtctgaagg atgttcggac ggcatttttt
     4321 atctttggcg gaagagggct tgcagccgct gtttgaaggc aatggggcgg atgctgctca
     4381 aaatgccgct gaggataatg atgcacatac cgagtatttc ctgccagaaa agctcttcgc
     4441 ccagaaaaaa tgcggcagac agggcggaaa agacgacggt catataggaa agcgaggcaa
     4501 ccgtgaattt gtcgccgact ttgtaggcgc gcgtcatcga cagttgggca atcagcgcgg
     4561 acacgccgat gcccgacaga taaaccgccg atggaaagga cagggtgtgc cagccggtca
     4621 gcgtcgccca aaccgacgac atcgccacgc cggttgcgga aaggtaaaag acgacgcgcc
     4681 agccgggttc gcccgccaaa gacagttcgc gcactttcaa atacgcccag ccggacatcg
     4741 cgccgcccgc cagcccggcg agtgccgccg gttcctgacc gctgcggaac gagggattaa
     4801 gcagcaatac cacgccggca aaaccaagga gcagcaccgc ctgcgtgtaa acggaaatcc
     4861 gttctttcaa aatcaggaag gaaaataccg ccaaaaaaat cgacgaggtg taactcaggg
     4921 taacgccggt tgtcaaaggc agatgcgtta ccgcgtaaaa cagcagcagc atcgcccccg
     4981 tcccgaccat actgcggttt aagtggtttt tccaatgggg cgtgcggaag gtgtcgcgcc
     5041 gcaatacggc ggcagcaccg agcgtaacgg ttgaaaacag catgcgccaa aagaccaatt
     5101 cgccgctgcc gagggcaaat tttgccgatg cctctttaat caatacgttc ataacggtga
     5161 agcaggccgc cgccaccagc atccagcccg atcctaaaat gtcttttttt gcggtatcca
     5221 taaacggtcg ttgcgataag gacggtcgga ttgtaaacct tgcggcaagg cttgtggaat
     5281 gtgtttttgc ctgcttctga tgccgaaatt ttatttttct tgccgaacaa tttgttttct
     5341 caaggcaaac ttgattataa tggcgggtat gaaaaaatac cttatccctc tttccattgc
     5401 ggcagtcctt tccggctgcc agtctattta tgtgcccaca ttgacggaaa tccccgtgaa
     5461 tcctatcaat accgtcaaaa cggaagcacc tgcaaaaggt tttcgcctcg ccccttcgca
     5521 ttgggcggat gttgccaaaa tcagcgatga agcgacgcgc ttgggctatc aggtgggtat
     5581 cggtaaaatg accaaggttc aggcggcgca atatctgaac aacttcagaa aacgcctggt
     5641 cggacgcaat gccgtcgatg acagtatgta tgaaatctac ctgcgttcgg cggtagacag
     5701 ccagcgcggc gaaatcaata cggaacagtc caagctgtat atcgagaatg ccttgcgcgg
     5761 ctggcagcag cgttggaaaa atatggatgc caaacccgat aatcccgcat ttaccaactt
     5821 tttgatggaa gtgatgaaga tgcagccctt gaaatgacgc ggtacgcaaa tgccgtctga
     5881 aagctttttc agacggcatt tgcgtttgaa gccccgattt atttttgccc gccttctttc
     5941 cggtattgcc ccggcgaaac gtgatattgc cgtttgaacg ccttgccgaa atgcgtttcc
     6001 gactgaaagc ccaccgacag cgcgacctcc aaaaccgaat ccggggtttt cttcagcagc
     6061 aatgcgcctt tttgcaggcg gatatggttc acaaaggcgt gcgggctgag tccgacttgg
     6121 cttttgaagc ggcgcatcag ttgcgcgcgc gacatattgg cggcggcaac cattttgtca
     6181 atattccatt cgtcttccgg tttgtctatc accttttgga tcaaatgtcc caaacgtttg
     6241 tcctgccaac ctttcaatac gcccgagagt tcgacatcct tatcctgttc gagataggcg
     6301 cgcaggataa gcaccagcag gacggacggt aatgcgttga ccacggaaac cgtccccgtc
     6361 aaaggttttt cgctttccag ttgcagcatt gaaaccacat actgcaaact tggatgggca
     6421 atgttcagaa aaacggtttc cggcagcccg ttcatcaaat cggcgtgggt gtcgtagcgg
     6481 aaacgggcgc aaaacaggct catatccagc ccgttgccgc actgtttgac cataaatgtg
     6541 ccgttttgcc gtatgtccgg ttgtaaactt tctccgtatt ttccgtcgtg gctcaacaca
     6601 tgacccaagc cgcgcgggaa aaatacaata tcgcccgtgc cgaccggacg cggggaagtt
     6661 tcgccgtcga tgcagagata accgctgccc gccgtaacaa tgtgtaccag cccttcgcat
     6721 tgcaaggttt catgccgtac cgaccattgt ccgcccaaaa ggcactgcac atccgcactg
     6781 cccgtcaatt gggcgagatc gaccagtttg tccagaatgt ccataaatct ttttgaacca
     6841 taaaatgaga tgattaaacg agaaacacaa ctgaataatt gcaataatac gcacatcaaa
     6901 aacagatacg caagcgcgta tcagggttaa acgcaagaga aaggaaaaga aaatgtttaa
     6961 agattggaaa gaacataccg cattggttaa aaaatcattc ggcgagctgg gtaaggcgca
     7021 tcctaaaatg ctgcaagcct acggcgcatt ggaacaggcc gccgccgccg aagcactcga
     7081 tgccaaaacg cgcgaactga ttgccatcgc cgttgccatc accacccgtt gcgagagctg
     7141 catcagcgtt cacgccgccg ccgccgccaa agccggtgcg accgacagcg agatcgcagg
     7201 tgcattggca accgccattg ccctgaatgc gggtgctgct tacacttacg ccctgcgtgc
     7261 attggaagcg gttgaaacgc aaaaataatc cgtttcggat aagaaatgcc gtctgaaaat
     7321 atttcagacg gcattttgcc tgttatgctt cccgatattt tgcagacaac atatgcagcc
     7381 gtttttttca gacggcattt gagaaacatg gaacaagtat gaaatatcac cgcttggcat
     7441 tgtttgccgc cataagctgc ctgttgttgt ccgccgtttt tattgcacct tacctgacgg
     7501 catttcacga acaagaaaaa atatttgaat atgcggattt gaccgttacc gcccccaacc
     7561 gcagcggacg ggcaatcaaa ttggaggcgg acgggcggca gtaccggctt tcctgctacg
     7621 ggttcgacag tttgtgcaca ggcggcaata tcggcagagc catcagggcg cggcaggtta
     7681 agattgtttt aagcgaaacc gtcggcaaag gttttttaaa cggcgttctg ttggaatacc
     7741 gcaacagtgg cagtgtctat agcaataaag atttttcccg cacggaagac cgccttgtcg
     7801 aagtgttggc acaacctgcc gttttcagcc tgaaaccggg tattttgctt ttattgcccg
     7861 ccattttcct gcgtttgaaa aaaatgtgaa aacagattgg cggggcgggg gaatgactat
     7921 gcggcaattt tacgttttgg gttatgagga taaccgttat aatcataatt ttacagtccg
     7981 cacagagaaa accgatgcct tggaacatcc ccatcttcct cacatggttg agggtcttgc
     8041 tcattcccgt actgattgtc cttttctacc tgccgttctc atggttttca gaagaagcag
     8101 tcaatgttgc cgccgccgtc atttttgccg ttgccgcctt gaccgactgg tttgacggat
     8161 ttttggcaag gttgtggaaa cagacttccg atttcggcgc attcctcgac cccgtcgccg
     8221 acaagctgat ggttgccgta tcgctgctgc tgctggtcaa actcgaccgg acctatgttt
     8281 tgttcgcgat gattatcatc ggcagggaaa ttaccatttc cgcattgcgc gaatggatgg
     8341 cgcaaatggg aaaaaggagc agcgttgccg tcgccgccgt cggcaagttc aaaaccgccg
     8401 cgcaaatgct ggcgattttc tttttgctgc tgaattttcc cgatttttac ggatttaatc
     8461 ttgttgttat cggcaacata ttgatgttta tcgcatcttt gctgacagtc tggtcgatgc
     8521 tgtattatct gaaaatggcg tggaaagaaa ttgcctgaaa aaaacataaa aatagcttga
     8581 cggtaaaaac ataatccata ataattgcgt cttcttcgat gtcggagagt gaaatcgggc
     8641 gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgcga gttcgag
//
LOCUS       DAXDSD010000087         8331 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXDSD010000087 DAXDSD010000000
VERSION     DAXDSD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.087"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..188)
                     /locus_tag="ACFB2Q_001676"
     CDS             complement(<1..188)
                     /locus_tag="ACFB2Q_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918661.1"
                     /translation="MFPPAEITGRGRVVLQQGFAGFGLYFHGVKFRRLNIPFKLPASC
                     RAVKYARMFAAVSADFGG"
     gene            313..996
                     /locus_tag="ACFB2Q_001677"
     CDS             313..996
                     /locus_tag="ACFB2Q_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFC0918662.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            1191..1460
                     /locus_tag="ACFB2Q_001678"
     CDS             1191..1460
                     /locus_tag="ACFB2Q_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918663.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(1816..3016)
                     /locus_tag="ACFB2Q_001679"
                     /pseudo
     CDS             complement(1816..3016)
                     /locus_tag="ACFB2Q_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3207..3296)
                     /locus_tag="ACFB2Q_001680"
     tRNA            complement(3207..3296)
                     /locus_tag="ACFB2Q_001680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3260..3262),aa:Ser,seq:cga)
     gene            complement(3373..3463)
                     /locus_tag="ACFB2Q_001681"
     tRNA            complement(3373..3463)
                     /locus_tag="ACFB2Q_001681"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3427..3429),aa:Ser,seq:tga)
     gene            complement(3546..4325)
                     /gene="yaaA"
                     /locus_tag="ACFB2Q_001682"
     CDS             complement(3546..4325)
                     /gene="yaaA"
                     /locus_tag="ACFB2Q_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC0918664.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTNQIQ
                     YLQNHVRLLSGLYGLMRPLDLMQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(4481..5668)
                     /gene="dapC"
                     /locus_tag="ACFB2Q_001683"
     CDS             complement(4481..5668)
                     /gene="dapC"
                     /locus_tag="ACFB2Q_001683"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC0918665.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDGLTVNPDNEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWERTKLVFVCSPNNPSGSVPDLDGWREVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVC
                     LYR"
     gene            complement(5740..6192)
                     /gene="dut"
                     /locus_tag="ACFB2Q_001684"
     CDS             complement(5740..6192)
                     /gene="dut"
                     /locus_tag="ACFB2Q_001684"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC0918666.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYTAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     GSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            6358..7069
                     /locus_tag="ACFB2Q_001685"
                     /pseudo
     CDS             6358..7069
                     /locus_tag="ACFB2Q_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            7066..7374
                     /locus_tag="ACFB2Q_001686"
     CDS             7066..7374
                     /locus_tag="ACFB2Q_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HFC0918667.1"
                     /translation="MKDLLSFPSFLLFSCMLAVTCPTRLIGFFALRNRTLSRRAQTVM
                     ETAPGCVLISVIAPYFVSDKPHELIAIALTAFAACRFSMLFTVLIGVGSSGISGWLMA
                     "
     gene            complement(7444..7917)
                     /locus_tag="ACFB2Q_001687"
     CDS             complement(7444..7917)
                     /locus_tag="ACFB2Q_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC0918668.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPQDNNQIIKSKLETFVLGYKMNPKIAKKYSVSVAFANTEKPRA
                     YRLVGVPKAGTGYTLSVWMNSVGDGYKCRNATSAQTYSETLSANTGCEAFSNRKK"
     gene            complement(7919..>8331)
                     /locus_tag="ACFB2Q_001688"
     CDS             complement(7919..>8331)
                     /locus_tag="ACFB2Q_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFC0918669.1"
                     /translation="EGEFQVLDLEYAADSKVTFSENCEKGLCTAVNVRTNDNGNEEAF
                     GNIVVQGKPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYKKGTGNVSKMPRYIIEYLGV
                     KNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
BASE COUNT         2206 a         2186 c         1904 g         2035 t
ORIGIN      
        1 ttgccgccaa agtccgccga tactgcggcg aacatccggg cgtatttgac ggcgcggcag
       61 gaagcaggca gcttgaacgg tatattaagc cgtctgaatt tcacgccgtg gaaatacagc
      121 ccgaagcctg caaagccttg ttgcaaaact accctgccgc ggccggtaat ctcggcaggc
      181 ggaaacaaaa aaagccccga gttcgtaagc gtatcagccc tagccgaatg gcccgaccga
      241 atcgaaagca aagcgaaaga cgaatgggca agggtgcgaa attaaaatac cggacaaatt
      301 aaagggttta atatggataa agagaaagtc ttagacaaaa tcaagaaatg tttggctttg
      361 ggtcggtcgg caaatgaaca tgaagccgca caggcgttaa ggcaggcgca ggctttaatg
      421 gaaaagtaca aagtaaatgc cgaggatatc gccttatcaa aagtctccga acagaaagcc
      481 gatcggaaaa tggcttttaa attggcaggc tggcaatggg gcgttgcaaa tatgattgcc
      541 gatatattcg ggtgcaaatc ttaccaacgc gggaaaacaa tgatgtttta cggcattgga
      601 aatcgagcgg aaacctcagc ctatgccttt gatgtagtct atcgtcagat ttccgccgac
      661 cgccgcaaat ttttgaaaac ctgccgggcg ggaaagccct cgcacagaac ttatcttgcc
      721 gaccgatttt gcggcggttg gattgcgagc gcttgggaaa cagtcaaaaa atttgagatg
      781 tcagatgaag aaaaagccat tatggacgga tacaaaaaga aagaatatcc ggatatggcc
      841 gaagcaagga caagagatgc gaaatcgtca atcctacaag ggtcaaaaat ggaatatgag
      901 gcattaaccc gaggaatgga atcgggtaag caagtgaagt tacactatgc cgtgaacggt
      961 acgggcttcg taaaacaaat cggagggcaa acatgaatcg atacggcggc ggatgcggaa
     1021 acttcggcgg attggggaaa ggatatgccg aatatgaaag acgcaattgc cgcatgccaa
     1081 gccgtaaaaa agcggaaacc cggacaaatc ggaatcaacg gaaagaggat tgaagacatg
     1141 gaatacgaag tattcaataa acgcccgtca aaaaagcggt cggcgaagca atggagtctt
     1201 ccgacgtaca agggcggatg gaaacactgg catttgcaac cgccccgaaa aaaggcggaa
     1261 tccggaaatc cggattcggc cgcgttattc taatcaaaag cgggcatata gttattcata
     1321 acgctgatgg cgacagcttt gccaccggct acggcgtgat tgccgccgac atcgtcagtc
     1381 ccgatttgaa aacagacttt gacatattct tcccgccgga aagcttgttg cgatataaaa
     1441 aaatcgggaa gaagaaatag gcagacaaac gacagagccg taaaaacggc gcaagattaa
     1501 accgaacagg agagtaaaaa aatgaacatc accaaaccgg gcgcaaggat agaccgcccg
     1561 accatccgcg aactgattgc ctacgcaacc tgccgcaacc accccatttc aaactcgaca
     1621 ctgctgcgta tggaaaaaga cggacggata ccgtgccgtc tgaacctccc tcgcatcccc
     1681 cgtatgggac acccgcgaag ttttggaagc attagggttg cagcaataaa aaaacgcccg
     1741 attagggcgt tttcacataa tgcggaaaca gctttcttat ctacatttcc acataacgca
     1801 aaacacacaa aaaaatcatt ttccgttgtc ggcaagcctc aaaacatcat tcccccaccg
     1861 ttccggcgca tccttacgct cctcccaaag ctccgagcgg ttgtaagccg tttgcgtcct
     1921 cgtccccaac gaatgggcaa gcagcttttc cgccacatca ttatacacct tcagaacctc
     1981 gcgcaaataa gtacgcgcaa gcgagcgcaa accgtgcgcc gtcgtatcca gccccgaccg
     2041 ccgcatcgcc acgcgcggac tttccttatt gatatgccct tgaaaattca cactttcaaa
     2101 cacaaaccgc ccgttcacat tcagcccccg cgcctgatcc aacacccacc gcatcgcccg
     2161 cgacagcggc acatcatgcc cccgccgctc ctttttccgt tcggcgggga tacgccaaac
     2221 ctccccgtcc aactccgacc actccatcaa cgccgcctcc cgaatccgcg tcatcgtcaa
     2281 cagcagccaa tagatacaaa accgcgttac aggatggata tgcaccctgc ccgcataaac
     2341 cgcaaattca ttgcgctgct ccaaaaaatc aatcaggcgc ggcaattcgg acggactcaa
     2401 cgccgtcatg tttttggttt tcacccgttc aaacacaccc gaaccgattt gcgcgacagg
     2461 gttgatttcc atcattcccg aaccgaccgc aaacgcaaac gcaaacacca tcttcagact
     2521 gtttttcgtt ttgcgcaacg tatcaacgat accgcgcgcc tccatcacac gcaggcagcc
     2581 gaccacgtcc gccgtcctga cttgacggat atcaagattg ccgatagccg gaaaaaccca
     2641 ccgttcaaaa ttccgcataa cctgtccggc atacttttca gaccgcccct tcgaccaacg
     2701 cacaaaccaa tcacgcgcca ccttctcaaa agcaaaatcc gcccgcacct tcttattgac
     2761 gacattttcc ccgtgtgccc gttttcggcg cacctcctcc cgccattccc gcgcctcagc
     2821 cagcgagaaa tcaggatacc gccccagcga aattgtctgt tgccttccgt cctgcataaa
     2881 cgacagcttc caactccgtc cgcccgtcgg gtagacccac aaagccagcc cccccgtccg
     2941 acaacttata cagcttatcg cgcggcttcg catttttaac ctgatttgcc gacagcggcg
     3001 taatgatttt cgccattatg gtaaattccc gtttgtccaa aatttaccat ataaattacc
     3061 ataaaaaaat gcaataagaa gatgccgcaa gattgcaaat gatttgcctt gaagcgttat
     3121 gataggtcaa gccgatgatt tttatatggt aatcaacaaa acttgaacaa aaaagaaccg
     3181 ccctgaatca gggcggttat gttttctggc ggaaacggtg ggattcgaac ccacggagga
     3241 tttgcaccct cagcggattt cgagtccgct gcattcagcc tctctgccac gtttccgata
     3301 atgcagtaaa accaaataaa aataacaata ttcgcggcta ttgcattttt atttggtttt
     3361 acgctcaaaa attggcggaa gcggtgagat tcgaactcac ggagggctat caaccctcga
     3421 cggttttcaa gaccgttgca ttaaaccgct ctgccacgct tccgtcttct tgaagagtag
     3481 gaataataat gaagtttata cattttgcca agcacttttt ttgggaaata cttaatttat
     3541 tgttttcact ttatttgttc cgaacgcata aacacccatt cgctttcatt ggaagccgcg
     3601 tcattgaatg cgtagccttc gtaattaaaa tttttcagca tttcaggatc ggtaatattg
     3661 tgttctgccg catagcgcac catcaatccg cgcgcgcgtt tggcatagaa actgatgatt
     3721 ttatatttac cgtttttttc gtctttaaat atcggggtaa tcagccgcgc ccgaagtttt
     3781 ttcgtgttga cggacttgaa atattcctgt gaggcaaggt tgacaagcgt attgctgcct
     3841 gcttgggcaa gcgtgtcatt taaaaggttg gtaatgatgt cgccccaaaa ctcatacaaa
     3901 ttcttgccgc gcaaattggc aaatgccgtt cccatttcca ggcggtaggg ctgcatcaaa
     3961 tccagtggac gcataagacc gtacaagccg gacagaaggc ggacatggtt ttgcagatat
     4021 tgtatttgat tggtattcaa tgtgtttgca tccatacctt cgtaaacatc gccgttgaac
     4081 ataaagactg cctgtttggc gttttccggc gtaaacggcg tgtgccacgc tgcatttcgt
     4141 tcggcgttta gaagtgcgat tttgtcggaa acgtgcatca actctgcaat ttgttgaggg
     4201 gcaagctccc tcagttggcg catcaggatt tcagattccg ccaaaagatc gggttgggta
     4261 aactcgctga cagggcaagg gtctttttcg ttaaggtttt tcgcagggga aaggacaaaa
     4321 aacataatcc gctcggtata gtgtcaaacg tcggaatttt agatttaaaa gcatcctcag
     4381 gcaagccgat tgattgtgat gtaaataccg ttataataaa tacatcttga tagtattaaa
     4441 aaaagccgtc aggaaaagtc cggacggcat tttttattct ttaccgatat aagcaaacaa
     4501 tggtttccgc agctttgaca caagttgcga catctgcaac caaagcaatc cgcacataac
     4561 cttccccggg attgccctgt tcggtatccc gtgccaaaaa gcgtccgggc aatacttgga
     4621 tagcggcttt ttgccataaa ttgcgtgcga atgccaaatc gtcgccatcg gggactttca
     4681 accagatgta aaacgaggca tccggtaatt taacgtcaaa tacctgttgc aaaatgggaa
     4741 taacacgctc aaatttttcc tgatacaggc ggcggttgtc gataacgtgt tgttcgtcat
     4801 tccaggcggc aatactggca cgctgcacgg gaatgctcat tgcgctgccg tgataggttc
     4861 tgtaaagcag aaagttctta agcagttccg catcaccggc gacaaaaccg gaacgcaggc
     4921 ccggaacatt ggaccgcttg gacaagctgg taaacataag cagtttttgc ctgcttcgcc
     4981 ccgactgtgc ggcggcctgc aggcagccca aaggtttgtt gccgtcaaaa tagatttcgg
     5041 aatagcattc atccgaggca ataatgaaac catatttatc ctgtaaatca aaaacttctc
     5101 tccatccgtc caaatccggc acgctgccgc tggggttgtt gggcgagcag acgaacacca
     5161 gtttggtgcg ttcccaaacc tcttcggaaa tactgcgcca atcggggttg aaagacggcg
     5221 cggggcaatt ggcaaaatgg atttcaccgc cgcccaaaag tgtcgcacct tcataaatct
     5281 gataaaaggg attcgggctg acgattacgg gttttaggcc gtctgaaaca gggttcaaca
     5341 cggtttgaac aaaagaaaac aacgcctccc tactgcccaa aaccggcaga atttcattat
     5401 ccggattgac cgtcaagccg tcataacggc gttttaacca gttcgcacac gcctgacgca
     5461 gttcaggcag acccgcagtc agcggatatt tttccaactc gcgcaatgag gcggtcagcg
     5521 catccgtaat gactttcggt gtcgggtgtt tcggttcgcc gatatgcagt gggacggctt
     5581 ccatgccttc aggcgcggaa atgccctgca tcgcctcgcg cagtcgggca aagggatatg
     5641 gtttgagctg cttgagtaag gtattcatga ttttctccgg ttatcggtat atcggaagat
     5701 atgccgcctg aaccgtcctt cagacggcat tctatatctt tagtgagaac ccgtactgcc
     5761 gaagccgccc tcaccccggc tgcttccgac aaattcctcg acacgtttga agcccgcctg
     5821 tacgaccggc acgataacca tttgtgcgat acgctcgaac ggtttgacag taaacggttc
     5881 gctgccccta ttccataacg acaccttcaa ttctccctga taatcggagt cgattaagcc
     5941 gaccaaattg cccaagacaa tgccgtgttt atgccccaag ccggaacggg gcagcaaaac
     6001 ggcggtatat gcgggattcg ccaaataaat tgccaaacct gtcggcacaa gaaacgtttc
     6061 gcccggctgc aaaacgactt cctcatccaa acaggcgcgc aaatccaaac ctgcagaacc
     6121 ctccgtcgca taggcaggga taaaatccgc catcctctcg tccaacactt tcatttctac
     6181 ttcaatattc atcgtcctgc tcctgcatac ggccggtttc aaagattgga gattatacac
     6241 aagccgtgtt tacgctgaaa cacaacaaac tgcttttgtt ttgaaaccat cctcgactgt
     6301 caaaaacatt ctgcctgtgt atgataccgc cccgtctctt catatataaa cattaacatg
     6361 acgcacacag cctctccacg tgatgaattc atacgcggca tcaaagaaag ttcgcccatg
     6421 ctgattgggc ttttgccttg ggcattgata ctcggaatgc agggcgggca aaaaggtatg
     6481 ggccggctgg aaatgctgct gatgacgggg atgaacttcg ccggcggctc cgaatttgcc
     6541 acggtcaacc tgtgggcgga acctctgccg atactgccta tcgccaccat aacctttatg
     6601 attaattcgc ggcatatcct gatggggggc ggcgcttgcc acgcacatga aagaaatacc
     6661 gctgaaaaaa gccgtgcccg cgctgctttt tatgtgtgat gaaagccggg ccatggcatt
     6721 ctccgaaatc caaaaacgga aagcagccgg tttgcctgca ttcaatatgc ctttttatat
     6781 gggcgcatgc tttatcctct acattacctg gatagggttt gccgccctcg gcgcggcagt
     6841 agggcccgtg ttcggtaatg tggccgcttg ggggttcggt atggcgtttc ctgccgtatt
     6901 tttggtttta ctgaggggaa tgtggaaaag tttttccgca tcaagacttt ggtttgtcaa
     6961 cctgtttgtc gcatccgccg tttacctgac cgttgacggc gcgtggtatg ttcccgccgg
     7021 cacgctgtcc ggcctgttgt ccgcctattt ttggaatggg cggaaatgaa agatttattg
     7081 tcgttcccat cattcctgct gttttcctgt atgcttgccg ttacctgccc tacccggtta
     7141 atcggctttt tcgcattgcg taaccgaacc ttaagccgcc gcgcccaaac cgttatggaa
     7201 accgcgccgg gctgcgtcct catttccgtt attgccccct attttgtctc cgacaagccg
     7261 cacgaactta ttgccatcgc gttaaccgcc tttgcggcct gccgtttttc tatgttgttt
     7321 accgtattga taggggtcgg ctcgtcaggc atctcgggat ggttgatggc ataattcatt
     7381 tcaaaatgcc cataaaaaat accgtctgaa aattgatctt cagacggtat tggcaaaaca
     7441 accctatttt ttacgattag agaaagcttc acagccggta tttgcggaca aggtctccga
     7501 ataggtctgg gcagaagtgg cattacggca tttgtatccg tcgcccacgc tgttcatcca
     7561 taccgacaag gtataacccg tccctgcctt cggaacgccg accaacctgt atgcccttgg
     7621 tttttccgta ttggcaaatg ccaccgaaac actatatttt ttggcaattt tcggattcat
     7681 cttatagcct aagacaaatg tttccagttt gctcttgatg atctgattat tgtcctgggg
     7741 atttttcaaa ataaactgtt tgagaacatt gttgataccg accatctccg tataaagctg
     7801 ggactgatag cctttttcaa tataactttg ataagaaggt atggcaatga cgctgataat
     7861 gccgagtatc gcgacaacta tcatcatctc aatcaatgta aacccttttt gttccatttt
     7921 attgctcatc attattgcct acataagatt gaaggacgac cacggtattg gcattcttac
     7981 cccaagcctt ggcagtaacc cgataaacat tttgtccgtt cttcacgcct aaatattcga
     8041 taatatagcg cggcattttg ctgacgtttc ccgtaccttt cttatattcc actccctgat
     8101 tgtcaatgca caggccggta gaatttttgc cagactttgc agggcaagaa cgcttcaccg
     8161 cctcaacggt gggcttgcct tgcaccacga tattgccaaa agcctcttca ttaccattat
     8221 catttgtccg cacattcact gcggtacaca gacctttttc acagttttcg ctaaacgtaa
     8281 ccttactgtc cgcagcatat tccaaatcca aaacctgaaa ttcgccctcc c
//
LOCUS       DAXDSD010000088         8244 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXDSD010000088 DAXDSD010000000
VERSION     DAXDSD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.088"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..816)
                     /gene="cysW"
                     /locus_tag="ACFB2Q_001689"
     CDS             complement(<1..816)
                     /gene="cysW"
                     /locus_tag="ACFB2Q_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC0918670.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAVLGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAVKLTLITALIAVPVNAVLGVAMAWLLTRFDFLGKQLLTT
                     LLDLPFSVSPAVAGLMFVLLFGAHTALGSRLEAQGIRIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITK"
     gene            complement(1005..1834)
                     /gene="cysT"
                     /locus_tag="ACFB2Q_001690"
                     /pseudo
     CDS             complement(1005..1834)
                     /gene="cysT"
                     /locus_tag="ACFB2Q_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
     gene            2020..2352
                     /locus_tag="ACFB2Q_001691"
     CDS             2020..2352
                     /locus_tag="ACFB2Q_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918671.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            2682..3191
                     /locus_tag="ACFB2Q_001692"
     CDS             2682..3191
                     /locus_tag="ACFB2Q_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC0918672.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(3421..4002)
                     /locus_tag="ACFB2Q_001693"
     CDS             complement(3421..4002)
                     /locus_tag="ACFB2Q_001693"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC0918673.1"
                     /translation="MEHKLPQLPYGPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPVGGLDLISTFNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLEGFWEIVNWDEVAKRFAA"
     gene            4165..5568
                     /gene="dnaB"
                     /locus_tag="ACFB2Q_001694"
     CDS             4165..5568
                     /gene="dnaB"
                     /locus_tag="ACFB2Q_001694"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC0918674.1"
                     /translation="MNDYAAMPPEGREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSITKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPMFIDETPNLTALELRARARRLARRFDGRLGLI
                     VIDYLQLMAASGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKVILTWMGQ
                     FTKFGNAAYIPESAAED"
     gene            5825..6487
                     /locus_tag="ACFB2Q_001695"
     CDS             5825..6487
                     /locus_tag="ACFB2Q_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC0918675.1"
                     /translation="MCTRKQQGFTLTELLIVMAIAAIMATIALPNMSGWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKDGASNNRCDFSKKGWGMLAFGDKNDNK
                     AYDGDATDVFLRSVVLNDTDDSRINYTFNHIAFGSSQPKADRVVWTFNQNGTFGYLPD
                     QNLKNNSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCRKNDTRAVC
                     KH"
     gene            6519..7133
                     /gene="pilV"
                     /locus_tag="ACFB2Q_001696"
     CDS             6519..7133
                     /gene="pilV"
                     /locus_tag="ACFB2Q_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC0918676.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTGPYTPTPSGGDFKLNNN
                     NLISKKDLAKAQLDRFDYELRNALPDAVAIHYAVCKDSSGGAPTLSGGTFSPNCDNKA
                     NGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGHE"
     gene            7130..8089
                     /locus_tag="ACFB2Q_001697"
     CDS             7130..8089
                     /locus_tag="ACFB2Q_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC0918677.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSVIVLIAVVSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTEKDVVSDVAQKNRL
                     FSLKMNSTNKVISNGTDNKLIPIAESLNIRYQGFTHRPNALIFQYGIDDVNASADTTV
                     VSSCAAISKPDKQILNLEDVKKELKIVGQGKEQNGNIARQRHVVNAYAVGRFGNEEGL
                     FRFQLNEKGEWGNPQLLAKKIKRMRVRYIYVSGCPEDEDAGKEEQFKYTDKFDSSVTP
                     AGVEVLLDSGSDAKIAASSDNIIYAYRINATIRGGNVCANRTL"
     gene            8068..>8244
                     /locus_tag="ACFB2Q_001698"
     CDS             8068..>8244
                     /locus_tag="ACFB2Q_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC0918678.1"
                     /translation="MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLAL"
BASE COUNT         2132 a         1956 c         2155 g         2001 t
ORIGIN      
        1 tttggtgatg atgttctgca ccgccagcgt cgccagtgct aaaagtgcca atacgccgga
       61 gagggagaat gcgccggtga agttgtattc gttgtagaag atttcgacca aaagcgggat
      121 agtgttggtt tcgccgcgta tgtgtcccga taccacgctg accgcgccga actcgcccat
      181 cgcacgggcg ttggtgagga tgatgccgta gagcagcgtc catttgatgt tgggcagggt
      241 aacgcgccaa aacatctgcc aaccgcttgc gccgagtatc aatgccgcct gttcttcgct
      301 gtcgccctgt gcctgcatta aagggatgat ttcgcgtgcg gcaaagggaa aggtaacgaa
      361 cagcgccgtc aaaacaatgc cgggaatggc gaagataatc cgtatgcctt gcgcttccag
      421 ccggctgccc aacgccgtgt gcgcgccgaa caataagacg aacatcaaac cggcaaccgc
      481 gggcgatacg gaaaacggca aatcgagcag ggtggtcagc aactgcttgc cgagaaagtc
      541 aaaacgggtc aacagccacg ccatcgccac gcccaagacg gcattgacgg gaacggcaat
      601 cagcgcggta atcagcgtca atttgacggc agaccacgct tcgggatcgc ttaaggattt
      661 caggtacaaa tcccaaccgc cttttaaggc ttcgtaaaac acggcgacga gcggcacgac
      721 cagcatcagc agcagaaagc ccagcacggc ggcaatcagc aacatgcgca gccggcgcgg
      781 ttcggtcagg ttgggattgg cggaataggg tttcatggcg gtggtgttct ctggtttgtt
      841 gtttaaggtg ttcggatgcc gtctgaaagg gattgagggg tttcagcttt cgtcggtcgg
      901 attcttgaat ccgacaaggt tgctgaatcg caaatgtttg tcggatacaa gtatccgacc
      961 tacgaattct gtgttttggg gagcggattc gttttcagac gacctcaacc cttcgcaccc
     1021 gaacgcctgc ccaacgccca ctgcagcacg ttcagcgcaa acagaatcac aaacgaaacc
     1081 agcagcataa acaacgccac cgccgacgcg ccctgcgcgt cgaattgttc cagcttgccc
     1141 gtaataatca gcggcaggat ttcggaaatc atcggaatgc tgcccgcgat aaaaatcacc
     1201 gaaccgtatt cccccgttgc ccgcgcgaac atcatgcccg cgccggtcaa gagtgccggc
     1261 gtgatttccg gcagcagcac gcggcggaac gttgtgaaac ggtttgcgcc caaagtcgcc
     1321 gccgcttcct catattcgcc cgacaattcc ttcaataccg gctgcacggc gcggacgata
     1381 aagggcaggc tgacgacaac cagcgcaatc caaatgccga cgggcgtaaa cgcgattttg
     1441 atgcccaaag gttcgaaaaa acggcctatc caaccgttgg gcgcatacag ggccgccaac
     1501 gcgatacccg taaccgccgt cggcagcgca aacggcaaat caaccagcgc gttcgccagg
     1561 cccttgcccg gaaattcata gcgcaccaac acccaagcca ccagtgtgcc gaacacgaca
     1621 cattggtcag catcgcataa aacgacatcc gcaaactcag ccgcaccgcc gccagcacgt
     1681 tcggctcggc aatcgtgttc cgaaagccgc cccagccgat ttccgccgcc atcatcgcaa
     1741 acggcaaaac caccagaagc gacaggcaca atacggtcag accgaggctg agtttgaagc
     1801 cgggcagtac gccgggtgtt ttgagcggta acataaaaca atgctgaaaa taaggaaaag
     1861 gaaggactac tttaacaatg ccgcctgaaa aacggaaaaa atggaaagtt tggtgcaaag
     1921 acgaatttgt tataaagcgg ttggcagttt ttcaagcggg cgcgatgttc taaaataacg
     1981 ctttgttttg acggaaaacc atcatataaa ggaacactta tgcagatttt atcttttcag
     2041 gcggacattg cggaacgtat gctggaaggt acggaaggcg agtcggtcaa cgaaaacgca
     2101 caatttgtcc gtacggacaa cggctattgg attgcgtggc atgaaggcgt ggcggcactg
     2161 cttgcgcccg atacgccgcc gggcattccc tgtttttggg tggaaggggc ggaaagcctt
     2221 gaagagttgt gcgcgatggt ggaacgcggc gagtttgacg aagtggaaga gtttgacggc
     2281 gatgacgacg cgtggctcga agcggctaaa gattgcggac accacggcga cgcttgcgcc
     2341 tgcggacatt aaaggcatgg caggcttgcc gcaaggggtg caaggctttg ccgtttttta
     2401 aataatcggc ggattgcatc tgtttgcaat gggcggaacg cttccctttg tattttattg
     2461 aataaaaatt atcttggtat tataataagg cagcatcaat tattttggga ctgcaacaaa
     2521 cgcaaagcat tgatttgcgt ttgttgcaaa cttattatta tagcaggttg cggcgcggac
     2581 ttgatgggaa ttgtttatag agcatgatag tctgaaacat attaattaaa aataccatta
     2641 atccgattta tatttatttc aatttcaatg gaaaaacatc aatgacaatg attttaagca
     2701 ttttaagcct gttttttatc attagactgt tatttttagc cgtctctatt aaacatgaaa
     2761 aagccttgat tgccaaaggg gcgaaacaat acggaaaaac caattccacg gtgcttgcgg
     2821 cagttcatac gctttattat ttggcgtgtt ttgtttgggt atggctttct gacactgctt
     2881 ttaatggcat atccttgatt ggtacactga cggtgatggc ttcatttgtg atattatcat
     2941 tgattattaa gcagttgggg gagatttgga cggttaaaat ctatattttg ccaaatcatc
     3001 aaattaatcg ttcgtggttg tttaaaacat tccgccaccc gaattatttt ttaaacatca
     3061 tacccgaact gattggcatc gccttattat gtcaagcgtg gtatgtttta ttgattggcc
     3121 tgcccattta tttgctggtc ttatttaagc gtatccgaca agaagaacag gcgatggcaa
     3181 cactttttta acccgtttca tcaattaaaa atatatcgac aaagagaacc gtctatcaat
     3241 taaatccatt cttatcattg gggcaggctt attgtcattg ggcaagccgt gacaaaagcc
     3301 cgaacctgca aaacttatct ataaggctgc atcagtgtgt attgaaaccc cgcctttttg
     3361 acaacaaatg ccgtctgaag ccttcagacg gcatttgttc ggtttggcaa agccggcggc
     3421 ttatgcggca aaacgtttgg cgacttcgtc ccagttgacg atttcccaaa aaccttccag
     3481 gtagttgggg cggctgttgc ggtagtcgat gtagtaggcg tgttcccaca cgtcgcaagt
     3541 caagagcggc gtgttttcag tggtcagcgg cgtagcggcg ttgaaagtgg aaatcaaatc
     3601 caatccgccg acaggggttt tcaccagcca cgcccaaccg gagccgaacg taccggccgc
     3661 gcaggcattg aacgcttctt ggaatttttc gaagctgccc catttcgcgt cgatggcggc
     3721 ggccagttcg ccggcaggct tgccttggcc tttgggcgtg aaacccagcc agtagaaggt
     3781 gtggttccaa gtttgagccg cgttgttgaa cacgccgccc gaagattttt tcacaatctc
     3841 ttccaaaggc aggttttcaa attcggtgcc tttgatttga ttgttcaggt tggtgatgta
     3901 ggtttgatgg tgtttgccgt agtggaattc caaagtctct ttgctcagat gcggggacaa
     3961 tgcgtccggc ccataaggca gttgcggcag cttatgttcc actttgtgct cctaatattg
     4021 ttttaaatgt tgtattttgg cagtgttgct gcaaacgaat cggcagctca cgcattctac
     4081 ctgttttgag tgatggaaac caattaaacc tgctttgcgt tataatagaa gattgcaatt
     4141 tcggcacgac agattggatg taccatgaac gattacgcag ctatgccgcc tgaaggccgt
     4201 gaggtcggcg cattgtcgct gcctccgcat tcgatggagg cggaacaatc cgttttgggc
     4261 ggtttgatgc tggaaaatcc ggcttgggac aggattgccg atgtggtttc gggagaggat
     4321 ttctaccgcc acgaacaccg cctgattttc cgatccatta ccaaattaat caatgagggc
     4381 cgtcccgcag atgtgattac ggttcaggaa gatttgcagc ggaacgaaga attggaagcg
     4441 gcaggcggat ttgaatatct gattacactg gcgcaaaaca ccccgtctgc cgcaaacatc
     4501 cgccgctacg ccgaaatcgt acgcgagcgt tccattatgc gccaactcgc cgaagtgggg
     4561 acggaaatcg cccgcagcgc atacaatccg caaggcaggg acgcggggcg gcttttggat
     4621 gaggcggaaa acaaagtgtt tcaaatcgcc gaaagcaccg ccaaatccaa gcagggcttt
     4681 ttggagatgc ccgatttgct gaaagaagtc gtacagcgca tcgatatgct ttattcgcgc
     4741 gacaatcccg atgaagttac cggcgtaccg acagggttca tcgaccttga taaaaaaacc
     4801 tcgggtctgc agcccggcga cctgattatc gtcgccggcc gcccgtctat gggtaaaacc
     4861 gcgttctcca tcaatattgc cgagtatgtc gccattgaaa aacatttgcc cgtcgccgtt
     4921 ttctcgatgg aaatgggcgg tgcgcagttg gtcatgcgta tgctcggctc ggtcggacgg
     4981 ctggatcaaa gcgttttgaa aaccggcagg ctggaagacg aacattgggg gcgtttgaac
     5041 gaggcagtcg tcaaactttc cgacgcgcct atgtttattg acgaaactcc gaatctgacc
     5101 gccctcgaat tgcgcgcccg cgcccgccgc ctcgcccgcc ggtttgacgg cagattaggc
     5161 ttgatcgtca ttgactacct gcaactgatg gcggcatccg gccgttcgga caaccgcgct
     5221 tccgagcttg gcgaaatttc ccgttccctc aaagccctgg caaaagagtt gcaagtcccc
     5281 atcatcgccc tgtcgcaatt gagccgcact gtcgaacagc gcaccgacaa acgcccgatg
     5341 atgtccgacc tccgcgaatc cggtgctatc gagcaggatg ccgacctgat tatgtttatg
     5401 taccgcgacg aatactacaa ccaggactca cccatgaaag gccttgccga atgtatcatc
     5461 ggcaaacacc gcaacggccc ggtcggaaaa gtcatcctga cgtggatggg gcagtttacc
     5521 aaattcggaa atgccgccta tatcccggaa tcggcagcgg aagactgaat ggcatagtat
     5581 agtggattaa caaaaaccag tacggcgttg cctcgccttg ccgtactatc tgtaccgtct
     5641 gcggcttcgc cgccttgtcc tgatttttgt taatccacta taaagtaaaa aaaattatta
     5701 ggcaaaaacg ggtaaaatcg tttagaattg cctatgcggt tttcctaaaa ataaaagcgg
     5761 cgggatgcct tccatccggc ctgcagataa ttgaaaatac attacagttg acgtcctatt
     5821 gcttatgtgt acacgaaaac aacaaggttt cacgctaaca gagctgctca tcgtgatggc
     5881 catcgcagcc attatggcga cgatagccct ccccaatatg agtgggtgga ttgcatcacg
     5941 ccgcattgcc agtcacgcgg agcaggttgc caaccttttg cgtttctcca ggggcgaagc
     6001 cgtccggctc aatctccctg tctatatctg tcctgttcaa gttaaaaaag acggtgcgtc
     6061 caacaataga tgtgacttca gcaagaaggg gtggggaatg ttggctttcg gcgacaaaaa
     6121 cgacaataag gcatatgacg gcgatgcgac ggatgttttc ctccgcagcg tggtgttgaa
     6181 tgataccgac gacagccgga ttaattacac cttcaatcat atcgctttcg gttcgtctca
     6241 gccgaaagcc gaccgtgtag tttggacttt caaccaaaac gggacattcg gctatttgcc
     6301 cgatcagaat ctcaagaata attccaaatt tgtttattct gacggttata tccaaatcgt
     6361 gctgacagat gcgagagcag tttcagatgc cgataagaaa ttccgttcgg cggtggtttt
     6421 gattaacagc agcggcaggg tcgaagtttg tcgtaaaaac gatacgcgcg ccgtatgcaa
     6481 acattaatga ctgttttagt cggtttggga aataaaaaat gaagaataat gattgcttgc
     6541 gcctgaaaaa tccccagtcc ggtatggcgt tgatagaagt cttggtcgct atgctcgttc
     6601 tgaccatcgg tattttggca ttgctgtccg tacagttgcg gacggtcgct tccgtcaggg
     6661 aggcggagac acaaaccatc gtcagccaaa tcacgcaaaa cctgatggaa ggaatgttga
     6721 tgaatccgac cattgattcg gacagcaaca agaaaaacta taatctttac acggggccgt
     6781 acacccccac tccctctggc ggcgatttca agcttaataa taataatttg ataagtaaga
     6841 aggatttggc aaaagcccag ttggacaggt tcgattatga attgagaaac gccttgccgg
     6901 atgcggtagc tattcattac gccgtctgca aggattcgtc gggtggcgcg ccgacattgt
     6961 ccggcggtac tttttctcca aattgcgata ataaggcaaa cggggatact ttgattaaag
     7021 tattgtgggt aaatgattcg gcaggggatt cggatatttc ccgtacgaat cttgaggtga
     7081 gcggcgacaa tatcgtatat acctatcagg caagggtcgg aggtcatgaa tgaaacgtaa
     7141 aatgctaaac gtaccaaagg gcggttatga tggtatgaag ggttttacca ttgttgaatt
     7201 tctggttgcg ggcctgctca gtgtgattgt cctgatagcg gtcgtatcga gttactttac
     7261 atcccggaaa ttaaatgatg cggcaaacga gcgtcttgcc attcaacagg atttgcggaa
     7321 tgcggcaaca ttgattgtcc gcgatgcaag aatggcgggg agcttcggtt gtttcaatat
     7381 gtctgagcat accgaaaagg atgttgtttc cgatgtggcg caaaaaaacc gtcttttttc
     7441 cttaaaaatg aacagtacaa ataaagttat ctccaacggt acagataata aacttatccc
     7501 catagcggaa tctttaaata tcagatatca gggttttacc catcgcccta acgcattgat
     7561 ttttcaatac ggaatcgatg atgttaatgc aagcgccgat actaccgtcg tcagcagctg
     7621 tgccgcaata tcgaaaccgg acaagcaaat ccttaattta gaagatgtaa aaaaagaatt
     7681 gaagattgtg ggtcagggta aggagcaaaa tggcaatata gcgcgtcaaa ggcatgtggt
     7741 caatgcctat gcggtcggca ggtttggcaa tgaggaaggt ttgttccgct tccaattgaa
     7801 tgaaaagggc gagtggggta atcctcagtt gctcgcgaaa aagattaaac gtatgagagt
     7861 gcggtatatc tatgtttccg gttgtcctga agatgaagat gccggcaaag aggaacaatt
     7921 caaatatacg gataaattcg acagctctgt tacgcctgcc ggggtggagg ttttattgga
     7981 tagcggtagt gatgccaaga ttgccgcttc ttcagataat attatttatg cttaccgtat
     8041 caatgcgaca atacgcgggg gaaatgtatg cgcaaacaga acactttgac aggaatcccg
     8101 acttctgacg gacagagggg gtccgcactg tttatcgtgc tgatggtgat gatagtcgtg
     8161 gcctttttgg ttgtaactgc cgcccagtcc tacaataccg aacagaggat cagtgccaac
     8221 gaatcagaca ggaaattggc tttg
//
LOCUS       DAXDSD010000089         7878 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXDSD010000089 DAXDSD010000000
VERSION     DAXDSD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.089"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            1..223
                     /locus_tag="ACFB2Q_001699"
                     /pseudo
     CDS             1..223
                     /locus_tag="ACFB2Q_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749744.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
     gene            333..1010
                     /locus_tag="ACFB2Q_001700"
     CDS             333..1010
                     /locus_tag="ACFB2Q_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFC0918679.1"
                     /translation="MTFFKPFTVVLTASALALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLAGTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
     gene            complement(1284..2054)
                     /gene="xth"
                     /locus_tag="ACFB2Q_001701"
     CDS             complement(1284..2054)
                     /gene="xth"
                     /locus_tag="ACFB2Q_001701"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC0918680.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(2114..2395)
                     /locus_tag="ACFB2Q_001702"
     CDS             complement(2114..2395)
                     /locus_tag="ACFB2Q_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC0918681.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLSAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            2452..2718
                     /locus_tag="ACFB2Q_001703"
     CDS             2452..2718
                     /locus_tag="ACFB2Q_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918682.1"
                     /translation="MILIIFINCIINQLFISHCTGLYVPFPTIFANKRKDRLSDGIKL
                     PPPSFPPVLPPAGIIRIVQKMPYNRRTPCPADASAPSGKQNIVD"
     gene            complement(3019..3900)
                     /gene="nadC"
                     /locus_tag="ACFB2Q_001704"
     CDS             complement(3019..3900)
                     /gene="nadC"
                     /locus_tag="ACFB2Q_001704"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC0918683.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPCVRFQAEIQDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(3976..4914)
                     /locus_tag="ACFB2Q_001705"
     CDS             complement(3976..4914)
                     /locus_tag="ACFB2Q_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918684.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            5116..6228
                     /gene="nadA"
                     /locus_tag="ACFB2Q_001706"
     CDS             5116..6228
                     /gene="nadA"
                     /locus_tag="ACFB2Q_001706"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC0918685.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKRKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6280..7788
                     /gene="nadB"
                     /locus_tag="ACFB2Q_001707"
     CDS             6280..7788
                     /gene="nadB"
                     /locus_tag="ACFB2Q_001707"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC0918686.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQG
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         1712 a         2324 c         2225 g         1617 t
ORIGIN      
        1 tcctaaaccc accatccgcg cctgccgcac gtacacgtcg acaaaccgaa aatatggctg
       61 ctcggcggca tcgtcggcgc gtggggctgc cataaaatcg gacaccattt ctccctgccc
      121 gtttcggcat tattgccgat accgggtgca ggctcggtcg gctacgatgt gaaggtacgt
      181 ttttttgcgg atgtggctgc ggtttcggcg caaatcacga taaacccgcg ttgtttcggg
      241 aaaaatcggg taatttcagg ttaattgcac tttttcagac ggcatgattg cttatagttg
      301 tacggtttga catatgcacg gaaggaaacg ccatgacttt cttcaaaccc tttaccgtcg
      361 tgctgaccgc atccgcactc gcgctttccg gctgcgttgc cgaccccgta accggacagc
      421 agtccccaaa caaatccgcc atgtacggtt tgggtggcgc ggcagtgtgc ggcatcgtcg
      481 gcgcactgac ccacagcggc aaaggcgcac gcaattccgc gcttgcctgc ggcgcaatcg
      541 gcgcaggcgt gggcggctat atggactacc aagagcagcg tttgcgccaa aaccttgccg
      601 gcacgcaaat cgaaatccaa cgccaaggca accaaatcag gctggtgatg cccgaaagcg
      661 ttaccttcgc caccggcagc gcggcgttgg gcggcagtgc gcaatacgcc ctgaacactg
      721 ccgcacagac gctggtgcag tatcccgaca cgacgctgac catcaacggg cacaccgaca
      781 acacaggttc cgatgcagtc aacaatccgc tttcgcaaca ccgcgcccaa gcggttgcct
      841 actatctgca gacgcgcggc gtggcggctt cgcgcctgac ggtttacggc tacggttcgc
      901 atatgccggt cgcgtccaac gctacggttg aaggccgcgc gcaaaaccgc cgcgtcgaaa
      961 tcctcatcaa ccccgaccaa cgcgccgtca acgccgcacg gcacatgtaa cgcgccctgc
     1021 aacgccaatg ccgtctgaat gtttttcaga cggcatttcc gtattcgcaa gcacagccgt
     1081 accccgactg taatgctatg gataacgtct cgccgtttgc cgggaaaccg cagattgcag
     1141 ccggtggcgt gcaagtgggc ccaaccttgc agacgttacc gccataccgc gatacccctg
     1201 taacctcatc gtttaaacag atattcaatc aaaatgccgt ctgaaaccct gcgaagtttc
     1261 agacggcatt tgcctttccc gaattacaaa tcaaattctg ccgccacggg cgcgtggtcg
     1321 ctcggacgct ccagcgcgcg cgtctccaaa tcgatgcgga catccttcaa aacggccgcc
     1381 atttcggatg taaccaatat atggtcgata cgcaggccca gtttgcgttg gaacatcgcg
     1441 ccgcgatagt cgaaccaggt atagaacgcg ccttcggggt ggacttggcg caggctgtcg
     1501 gtcaatccca aatccagcag attttgaaac cactgccgtt cgacggacga acagtggatt
     1561 ttttcatacc atttttcagg gtcgtaacag tccgcatcgg caggcgcaat attgaaatcg
     1621 cccagcagta ccagtttgcc gtggcgggtc atttcgtcgc ggacaaactc cgtcagtgcg
     1681 gcaaaccact gttccttata tttgaatttg gggctgtcca aagcctcgcc gttgacgcaa
     1741 tagacattga tgacgcgcac gccgcctacg gtcgccgcaa tcacgcggcg ttgcgggtca
     1801 tccggcaggg aaggcaaacc gaaatgcacg tcctgcggca cgctgcggct gacgattgcc
     1861 acaccgttgt aggttttctg cccgctccaa acacagtgcc agcccatcat ctgcaaggcg
     1921 gcggccggaa atttgtcctg atcgagtttg agttcctgca aaaccaaaat atcgggcgga
     1981 ttgtcggcaa gcaggttttg cacctgcggc agccgcacat tgagcgaatt gacgttccaa
     2041 gtggtgattt tcataacatt tccgaaaaat gccgtctgaa acgttcagac ggcaaaagaa
     2101 aaggattcta acactatgcc gcgcgtttgt tttccaaatc gcggattgtc cgcagaatcg
     2161 ttgccgcgtc ttcggaaacc aggcggtatt cgataatgcg gtggtaacgc gtaaagtcca
     2221 ccagaccgcc cgccctcagg cggttcaaat gacaggaaac cgcggtggcc gacagggata
     2281 aggattttgc cagttcgacg atattgcgtt cgctgtccaa cagttggatc agtatcgcca
     2341 tacgttcggg atgcgccata agtttgagta tggtgtggag cggtattgtg ttcatcggta
     2401 ttcctaaaac cttttccgcc cgccaagcgg attgacgggc ggcagatatg gatgattttg
     2461 attatcttta taaattgtat tataaatcaa ttgtttattt ctcattgtac aggattgtat
     2521 gttccttttc ccactatttt tgcaaacaag cgtaaagaca ggctttcaga cggcataaaa
     2581 ctgccgcccc cgtcctttcc ccctgtcctg ccgccggccg gaatcatccg cattgttcaa
     2641 aagatgcctt acaaccgccg cacgccttgc cctgccgatg cctccgcgcc ttcgggcaaa
     2701 caaaatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtataaat
     2761 agtacggaac cggttcactt gatgcttcag caccttagag aatcgttctc tttgagccgg
     2821 ggcggggcaa cgccgtaccg gtttttgtta atccactata cctgcctgaa atgctgtttt
     2881 ttctaaagca tcacgacctt cacgaaaaat gccgtctgaa gaatatttca gacggcattc
     2941 cctccctgag gcaataaccg gattacggtt aagcggttcc ttacctgaca tatcagacag
     3001 ccgcccgcac cctgaaactc acgccacgaa atccaaacct atgtccaacg atcggctgct
     3061 gtgggtcaga tagccgaggg cgatgccgtc cacgccggtt tgcgccacgc gcttcaggcg
     3121 gtcgaagccg atgccgcccg atgcttcgca atagacggtg tgggggtggg cggtttgtgt
     3181 gtggcagcgg tttgccgctt ctttcagggt ttcgtcgtcc atgttgtcca gcaaaatccg
     3241 ttccgcgccc gctgcgatgg cttcgtccag ttgtgccaat gtgtccactt cgatttccac
     3301 gcaggtcaat ggtccgaccg cctgttttgc ctgccgaacg gcttgggcga tgctgccgca
     3361 ataggcgagg tggttgtctt tgatgagcac ggcgtcgtcc aaacccatgc ggtggttcgc
     3421 gccgccgccc gccctgacgg cgtatttttg caggacgcgc agcaggggga tggttttgcg
     3481 gctgcacacg atgtctgtac cgtattcggc gacctcggca acggcacgag cggtggcggt
     3541 ggcgatgccg cttaagtgcg tgaggtagtt gagcgcggtg cgttcggcgg cgagcagcgc
     3601 gcgggcgttg ccttcgacgg cagccagtgt ctgacctgcg cggacggctt gcccgtcttg
     3661 gatttcggct tggaagcgga cgcacggatc catcgtctga aaggcgaggc gcgccaagtc
     3721 cataccggcg ataacaccgt cttcgcggct gacgaggaaa agtttggcgg ttttgtcggg
     3781 cgcgatgacg gcggcggacg taatgtcgcc gcgcctgccc aagtcttcgc tcaaggcttg
     3841 ttccaccata gggcgcaaca aggtgtcggg cagggcaaag agggggtttt cagacggcat
     3901 ggggtttcct ttgttaaaaa agattgatgt tgcaccgatg gggcaaaact tcatttgatg
     3961 ccgtctgaaa acgggctaac ggctgcccag cggcagtccg atgtccgaaa tcagcctgtc
     4021 gggcaggacg tcgtcgcgga agcggcaaag ctgcgcggga cggcctttgc tgcccgatac
     4081 gcctgtatcc gacggctcga tgaggttttg ctgctgaatc tggcggcgga agttttgctt
     4141 gtgcagcaat ctgccgctga tggcttcgac gctgttttgc agttgcagca gcgtgaattc
     4201 gggcggcatc agttcgaaaa tcacgggacg gtatttgatt ttggcgcgca ggcgggacag
     4261 ggcggtcgcc aacacgcggc ggtggtcgtg gcgcatggtc tgccccgtga gcgcgaagtc
     4321 gaagtttgcc tgcggctcgg cggcctccgc tatcagaccg ctttcataca acatttcata
     4381 gcgttgcaaa acgtattctt ccgaccagtt ttccggttcg accccccaac acaaatgaat
     4441 atgcttgagc cgcttttggc ggacttcctc cgtgtccgcc gagtttgccc aaatgcgcag
     4501 gcggctgacg acggcgtcgc gctgcccgcc gtcggtgcgc aagtcttccc atgggaaata
     4561 gccgtagcag tcctgccatt tcgcgtcggg atgcaggatg ctatcggctg cctcgcgcac
     4621 cagccccaaa tagctgacgt acagcacggg catgccgtgt tcgttgcggc ggtgggtatc
     4681 gacaaaggtg taaagctgtt ccacatagcc cataggctgc gaagtctgct tggcgaccca
     4741 cagctttacg cccgcctgca gggaattgcg caggggggaa agcgggccgt tgggcaggag
     4801 cgtgccttgg gcgacggtca acacccgcag gccgccgtcg gtaacggcaa tcaatacggg
     4861 aaccagctcg acgatgcttt gcagcggagc ttcggcttcg gggtaggcgt ccatgctcgg
     4921 ctttctgcgg gtgggaaagg ctttattgta aaccaaagcg gtttgtcgga acgaagcggg
     4981 tcggttttgc ggtatcgcaa acctgttttc agacgacctt gcatattata ctcaagttga
     5041 gcataatgta aaaacagttt gaaaaacaga cggcaaacct tcatgccgtc tgaaaaccag
     5101 ctacaaggaa acatcatgca aaccgccgcc cgccgctcgt tcgattacga tatgcccctc
     5161 atccaaacgc cgacttccgc ctgccaaatc cgtcaggcgt gggcgaaggt tgccgacacg
     5221 cccgaccacg agacggcagg tcgtctgaaa gacgaaatca aggccttgct gaagcggaaa
     5281 aacgcggtct tggtggcgca ttattacgtc gatccgctga ttcaggattt ggctttggag
     5341 acgggcggat gcgtgggcga ttcgctggaa atggcgcgct tcggcgcgga acacgaagcc
     5401 ggtacgctgg tagtggcggg cgtgcgtttc atgggcgaga gcgcgaaaat cctctgccct
     5461 gaaaaaacgg tgctgatgcc tgatttggaa gcggaatgtt ctttggattt gggttgtccc
     5521 gaagaagcgt tttcagcgtt ttgcgaccaa caccccgacc gcacggtggc ggtgtacgcc
     5581 aatacttccg cagccgtgaa agcgcgtgcc gactgggtgg taacgtcttc ggtggcgttg
     5641 gaaatcgtgt cgtatctgaa atcgcgcggc gagaaactga tttgggggcc cgaccgccat
     5701 ctcggcgact acatccgccg cgaaacgggc gcggatatgc tgttgtggca gggttcgtgc
     5761 atcgtccata acgaattcaa agggcaggaa ctggcggcgt tgaaggcaga gcatcccgat
     5821 gctgtggtgc tggttcatcc cgaatcgccg caaagcgtca tcgaactggg cgacgtggtc
     5881 ggctcgacca gcaaactact caaagccgcc gtatcgcgcc ctgaaaaaaa attcatcgtg
     5941 gcaaccgatt tgggcatcct gcacgaaatg caaaagcagg cgcccgacaa agaatttatc
     6001 gccgcgccga cggcgggcaa cggcggaagc tgtaaaagct gcgcgttctg cccgtggatg
     6061 gcgatgaatt cgctgggcgg catcaaacac gccctgacag gcggacgcaa cgaaatcctg
     6121 ttggacagaa agctgggcga agccgccaaa ctgcctttgc agcgtatgct cgacttcgcg
     6181 gcaggactca aacgcgggga tgtgttcaac ggcatggggc ccgcctgatt tgccgtccat
     6241 atcgccgttt cagacgacct ctcaaacaag gacaacacca tgcaaaccga ttgcgacgta
     6301 ttgattgccg gaaacgggct ggcggcactg acgctcgccc tgtcgctgcc tgaatcgttc
     6361 cgcatcgtca ttttgtgcaa aaaccggctg gacgacaccg ccagccgtca tgcgcaaggc
     6421 gggattgcgg cggcgtggtc gggagaggac gacatcggaa aacacgtcgc cgatactttg
     6481 gaagcgggcg caggtttgtg cgacgaagcc gccgtccgca ccatcctgtc gcagggcaaa
     6541 ccggcaatcg aatggctgct ggcgcagggc gtggcgttcg accggaatca taacgacctg
     6601 cacctgacgc gcgaaggcgg gcatacctgc cgccgaatcg cccacgtcgc cgactacacg
     6661 ggcgaagccg tcatgcagag cctgattatc caaatacgcc gccgaccgaa catccgcgtt
     6721 tacgagcggc agatggcgtt ggacgttcaa accgaatcag gcgcagcgtg cggactgacc
     6781 gtcctcgact gccgaacgca agaaacctac cgcatccgtg cccgccatac cgtactcgca
     6841 ggcggcggcc tcgggcaaat ttacgccgcc accaccacgc cgcccgaatg cacgggcgac
     6901 gccatcgcca tggcgatacg cgcgggttgc gcaatcgaaa acctcgaatt tatccaattc
     6961 caccccacag gcttggcaag gccgtctgaa aacggccgca ccttcctgat ttccgaagcc
     7021 gtgcgcggcg aaggcggcat cctgaccaat caatcgggcg aacggtttat gccgcattac
     7081 gaccgccgcg ccgaactcgc gccgcgcgac atcgttgccc gcgccatcgc cgccgaaacc
     7141 gccaaacaaa cgcaagactt cgtctcgctc gacatcagcc gccaacccgc agcgttcgtc
     7201 cgtcggcatt tcccgtccat ccatcggcac tgcctgtccc aatgcggctt ggacatcacg
     7261 cgccaagcca tccccgtccg ccccgtgcaa cactacacct gcggcggcat ccaaaccgac
     7321 cccagcggca gaacctccct gccgcagctc tacgccttag gcgaaaccgc ctgtaccgga
     7381 ctgcacggag ccaaccgcct cgccagcaac tccctgctcg aatgcgtcgt taccgccagg
     7441 cttgccgccc aaggcatcgc ggacggacaa gcgttccaaa tcgaaccatt ccaaaggccg
     7501 tctgaaagcc cctccgccga aacagacatc ttttcaaacg acctccaaaa cacattcagc
     7561 cgccctgtcc tgcaaacgtt caaccaacgc catctcggca tcctccgcaa cgataccgac
     7621 ctgcaccgcg ccatcgacca actgcggctt tggaagcaaa accaagccga accgcacacc
     7681 gcgtccgaat acgaaaaccg caacctactc gaatgcagcc tcgccgtcgc ccaagccgca
     7741 tacaggcgga gacagaacat cggtgcgcat tttaatagtg attgttaagg ttggctggtt
     7801 agaaaaaaat cgttcaaata aatttgaata gcatggttaa gagattggca ttcagttttt
     7861 atggttgcgt aagttggg
//
LOCUS       DAXDSD010000090         7833 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXDSD010000090 DAXDSD010000000
VERSION     DAXDSD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.090"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1110
                     /locus_tag="ACFB2Q_001708"
     CDS             <1..1110
                     /locus_tag="ACFB2Q_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC0918687.1"
                     /translation="GCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGVPWQVALGAV
                     FISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGIIVANPATLV
                     GLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLNEFHGVVGEV
                     PGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLLVDGKLPRLK
                     RALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLMLACLMFSPLA
                     KSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYSIADGIAFGF
                     ISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
     gene            1733..2608
                     /gene="prpB"
                     /locus_tag="ACFB2Q_001709"
     CDS             1733..2608
                     /gene="prpB"
                     /locus_tag="ACFB2Q_001709"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC0918688.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRRNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGATPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFDQKLDKLFQK"
     gene            2693..3841
                     /gene="prpC"
                     /locus_tag="ACFB2Q_001710"
     CDS             2693..3841
                     /gene="prpC"
                     /locus_tag="ACFB2Q_001710"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC0918689.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPDSEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETVGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            3993..4781
                     /locus_tag="ACFB2Q_001711"
     CDS             3993..4781
                     /locus_tag="ACFB2Q_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC0918690.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            4858..7463
                     /gene="acnD"
                     /locus_tag="ACFB2Q_001712"
                     /pseudo
     CDS             4858..7463
                     /gene="acnD"
                     /locus_tag="ACFB2Q_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
BASE COUNT         1857 a         1955 c         2070 g         1951 t
ORIGIN      
        1 ggctgttttg tcatgggttt tatcggcaac tatccgattg cgcttgcccc ggggatgggg
       61 ctgaatgcct atttcacctt tgccgtcgtt aagggtatgg gcgtgccttg gcaggtggcg
      121 ttgggtgcgg tgttcatttc cggtctgatt ttcatcctgt tcagcttttt taaagtcagg
      181 gaaatgctgg tcaacgcact gcctatgggt ttgaaaatgt cgattgccgc cggtatcggt
      241 ttgtttttgg cactgatttc cctgaaaggc gcaggcatta tcgttgccaa tccggcaacc
      301 ttggtcggct tgggcgatat tcatcagccc agcgcactgt tggcattgtt cggttttgtc
      361 atggtggtcg tattggggta tttccgcgtt caaggcgcaa tcatcatcac cattctgacg
      421 attaccgtca ttgccagcct gatgggtttg aacgagtttc acggcgtggt cggcgaagta
      481 ccgggcattg cgccgacctt tatgcagatg gattttaaag gtctgtttac cgtcagcatg
      541 gtcagcgtga ttttcgtctt cttcttggtc gatttgtttg acagtaccgg aacgctggtc
      601 ggcgtatccc accgtgccgg actgctggtg gacggtaagc tgccccgcct gaaacgcgca
      661 ctgcttgcag actctaccgc cattgtggca ggtgcggctt tgggtacttc ttcaaccaca
      721 ccttatgtgg aaagcgcggc gggcgtatcg gcaggcggac ggaccggcct gacggcggtt
      781 accgtcggcg tattgatgct ggcgtgtctg atgttctccc cattggcgaa aagtgttccg
      841 gtatttgcca ccgcgcccgc actgctttat gtcggcacgc agatgctccg cagtgcgagg
      901 gacattgatt gggacgatat gactgaagcc gcgcccgcgt tcctgaccat tgtcttcatg
      961 ccgtttacct attcgattgc agacggcatc gccttcggct tcatcagcta tgccgtggtc
     1021 aaacttttgt gtcgccggac tggggacgtg ccgcctatgg tatgggttgt tgccgtattg
     1081 tgggcattga aattctggta tttgggctga ttgattcgat attaaaaatg ccgtctgaaa
     1141 ggttttcaga cggcattttg tttgccagat atatttaatt ttaaataaat aatataaaaa
     1201 tcaaatagat aataaactac atcggattac ttgaaaataa ttcattgttt tatatataaa
     1261 atattttata atttttcagg attttgatta ttgaaaattt ttctaaattt cctattaatt
     1321 ttatcggaaa caaataagtt aaaattaatt tatattaatc ataatgttaa aataaaataa
     1381 taaaattatg taattagtct ccttaaaaat gtttgacatt ccagtcttgt gttttagatt
     1441 accgaaaaat aaaactacat aatactacaa aggaacatta ctatgaaacc aattcagatg
     1501 tttttccctt ttctgaataa tccccttgtt ttcttcttgt ctgcggtttt gccgcataat
     1561 tccgaacggt ctgctgtttt tctttgattc gttttaaata tcaataagat aatttttccc
     1621 atatattttt aatgattggg atgcccgacg cgtcggatgg ctgtgttttg ccgtccgaat
     1681 gtgatggaag cctgtccata ctgaaaaaaa aagtctataa aggagaaata tgatgagtca
     1741 acactctgcc ggagcacgtt tccgccaagc tgtgaaagaa tcgaatccgc ttgccgtcgt
     1801 cggttgcgtc aatgcttact ttgcacgatt ggccacccaa agcggtttca aagccatcta
     1861 tctgtccggc ggcggcgtgg cagcctgttc ttgcggtatc cctgatttgg gcattaccac
     1921 aatggaagat gtgctgatcg acgcacgacg cattacggac aacgtggata cgcctctgct
     1981 ggtggacatc gatgtgggtt ggggcggtgc attcaatatt gcccgtacca ttcgcaactt
     2041 tgaacgcgcc ggtgttgcag cggttcacat cgaagatcag gtagcgcaaa aacgctgcgg
     2101 ccaccgtcgg aacaaagcca tcgtttccaa agatgaaatg gttgaccgca tcaaagctgc
     2161 cgtagatgcg cgcgttgatg agaacttcgt gattatggcg cgtaccgatg cgctggcggt
     2221 agaaggtttg gatgccgcta tcgaacgcgc tcaggcttgt gtcgaagccg gtgcggacat
     2281 gattttccct gaagccatga ccgatttgaa aatgtaccgt caatttgcag atgcagtgaa
     2341 agtaccagta ttggccaaca ttaccgagtt tggtgctaca ccgctttata cgcaaagcga
     2401 gttggctgaa aacggcgtgt cactggtgct gtatccgttg tcatcattcc gcgcagcaag
     2461 caaagccgct ttgaatgttt acgaagcgat tatgcgcgat ggcactcagg cggcggtggt
     2521 ggacagtatg caaacccgtg ccgagctgta cgagcatctg aactatcatg ccttcgatca
     2581 aaaactggat aaattgtttc aaaaatgatt taccgctttc agacggcctt tcaacaaatc
     2641 cgcatcgacc gtctgaaaaa taaaacccat aaaaatacaa aggagaaata ccatgactga
     2701 aactactcaa accccgacct tcaaacctaa aaaatccgtt gcgctttcag gtgttgcggc
     2761 cggtaatacc gctttgtgta ccgttggccg taccggtaac gatttgagcc atcgcggtta
     2821 cgacattcta aatttggcac aaaaatgcga gtttgaagaa gtcgcacact tgctgattca
     2881 cggccatctg cccaacaaat tcgagctggc cgcttataaa accaagctca aatccatgcg
     2941 cggcctgcct atccgtgtga ttaaagtttt ggaaagcctg cctgcacata cccatccgat
     3001 ggacgtgatg cgtaccggcg tgtccatgct cggctgcgtt catcccgaac gcgaaagcca
     3061 tcccgacagc gaagcgcgcg acatcgccga caaactgatc gccagcttgg gcagcatcct
     3121 gctctactgg tatcaatatt cgcacaacgg caaacgtatt gaagttgaaa gcgacgaaga
     3181 gaccgtcggc ggtcatttcc tgcacctgtt gcacggcaaa cgcccaagcg aatcacacat
     3241 caaagccatg catgtttcac tgattctgta tgccgaacac gagttcaacg cctccacttt
     3301 tactgcccgc gtgattgccg gtacgggttc ggatatgtat tcctgcatca ccggcgccat
     3361 cggcgcgctt aaaggtccga aacacggcgg tgcgaacgaa gtcgcctacg acatccaaaa
     3421 acgctaccgc aacgccgacg aagccgacat ccgcgaacgc atcggccgca aagaaatcgt
     3481 gatcggtttc ggccatccgg tttacaccat ttccgacccg cgcaacgtcg tgattaaaga
     3541 agtggcgcgc ggtttgagcc gggaagcggg cgacatgcgc ctcttcgaca tcgccgaacg
     3601 cttggagagc gtgatgtggg aagagaaaaa aatgttcccg aatctggact ggttctctgc
     3661 cgtttcctac caaaaattgg gcgtaccgac cgctatgttc acaccgctgt tcgtgatttc
     3721 ccgtacaacc ggttggagcg cacacgtcct cgaacagcgc aaagacggca aaatcatccg
     3781 tccgagcgca aactacaccg gccctgaaga tttggcgttt gtggagattg aagaacgata
     3841 attgaagaat gcaatagcag tttgttcttt atttcggtat gcaaagctga ggatttcaga
     3901 cgaccttgcc ttattggaaa ggttgtctga aataagttta atctaatagg agaagataat
     3961 cctgtattgg cgcaagtaac aggataagaa acatggaaga tttatacata atactcgctt
     4021 tgggtttggt tgcgatgatc gccggattta tcgatgcgat tgcgggcggg ggtggtttga
     4081 ttacgctgcc tgcactcttg ttggcaggta ttcctcccgt gtcggcaatt gccaccaaca
     4141 agctgcaagc agccgctgct acgttttcgg ctacggtttc ttttgcacgc aaaggtttga
     4201 ttgattggaa gaaaggtctc ccgattgccg cagcatcgtt tgcaggcggc gtggtcggtg
     4261 cattatcggt cagcttggtt tccaaagata ttttgctggc ggtcgtgccg gttttgttga
     4321 tatttgtcgc gctgtatttt gtgttttcgc ccaagctcga cggcagtaag gaaggcaaag
     4381 ccagaatgtc tttttttcta ttcgggctga cggttgcacc gcttttgggt ttttacgacg
     4441 gtgtgttcgg accgggtgtc ggctcgtttt ttctgattgc ctttattgtt ttgctcggct
     4501 gcaagctgtt gaacgcgatg tcttacacca aattggcgaa cgttgcttgc aatcttggtt
     4561 cgctatcggt attcctgctg cacggttcga ttattttccc gattgtggca acgatggcgg
     4621 tcggtgcgtt tgtcggtgcg aatttaggtg cgagatttgc cgtccgcttc ggttcgaagc
     4681 tgattaagcc gctgctgatt gtcatcagca tttcgatggc tgtgaaattg ttgatagacg
     4741 agagaaatcc gctgtatcag atgattgttt cgatgtttta aaccctttca gacgactcct
     4801 tcaaaacatc ggctgaaacc caaaccacaa gaaaaataga tccacaggag aaccgacatg
     4861 actgccaacc aaagttaccg caaaccgctg cccggtacgg atttggaata ctacgacgcg
     4921 cgtgcggcgt gtgaggacat caaacccggc tcttacgaca agctgccgta cacgagccgc
     4981 attttgtctg agaacttggt caaccgcgcg gacaaagtcg atttgccgat gctgcaaagc
     5041 tggctgggcc agttgataga agggaagcag gaaatcgatt ttccgtggta tcccgcgcgg
     5101 gtggtgtgcc acgatattct ggggcagacc gcgctggtgg atttggcagg cttgcgcgat
     5161 gcgattgccg aaaaaggcgg cgatcctgcc aaagtgaatc cggtggtgca gacgcagctt
     5221 atcgtcgacc actctttggc ggtggagtgc ggcggttacg atcctgatgc cttccgcaaa
     5281 aaccgcgaaa tcgaagacag acgtaacgaa gaccgtttcc acttcatcaa ctggacaaaa
     5341 accgcatttg aaaatgtgga cgtgattccg gcgggcaacg gcatcatgca ccaaatcaat
     5401 ctggaaaaaa tgtcgcccgt cgtccaagtc aaaaacggcg tggctttccc cgatacctgc
     5461 gtcggcacgg actcgcatac gccgcacgtt gatgcgctgg gcgtgatttc cgtggcgtgg
     5521 gcggtttgga agcggaaacc gtgatgctgg gacgcgcgtc catgatgcgc ctgcccgata
     5581 ttgtcggcgt ggagctgacg ggcaaacggc aggcgggcat tactgccaca gacatcgtgt
     5641 tggcactgac cgaattcttg cgtaaagagc gcgtggtcgg ggcgtttgtc gaatttttcg
     5701 gcgagggcgc gagaagcctg tctatcggcg accgcgcgac catttccaac atgacgccgg
     5761 agttcggcgc gactgccgcc atgttcgcca tcgacgcgca aactattgat tatttgaaac
     5821 tgaccggacg tgacgacgcg caggtgaaat tggtggaaac ctacgccaaa accgcaggct
     5881 tatgggcagg tggcttgaaa accgccgttt atccgcgcgt tttgaaattt gatttgagca
     5941 gcgtaacgcg caatatggca ggcccgagca acccgcacgc gcgttttgcc accgccgatt
     6001 tggcggcgaa agggctggcg aagccttacg aagagccttc agacggccaa atgcctgacg
     6061 gtgcagtgat tattgccgcg attacttcgt gtaccaatac ttccaacccg cgcaacgttg
     6121 tcgccgccgc actgttggca cgcaatgcca accgcctcgg cttgaaacgc aaaccttggg
     6181 tgaaatcttc gtttgccccg ggttcaaaag tagccggaat ctatttgaaa gaagcaggct
     6241 tgttgcccga aatggaaaaa ctcggcttcg gtatcgtcgc cttcgcatgt accacctgta
     6301 acggcatgag cggcgcgctc gacccgaaaa tccaacaaga aatcatcgac cgcgatttgt
     6361 acgccaccgc cgtattgtca ggcaaccgca acttcgacgg ccgtatccat ccgtatgcga
     6421 aacaggcttt cctcgcttcg cctcctttgg tcgttgccta cgcattggca ggtagcatcc
     6481 gtttcgatat tgaaaacgac gtactcggcg ttgcagacgg ccgcgaaatc cgcctgaaag
     6541 atatctggcc gacagacgaa gaaatcgatg ccatggttgc cgaatatgtg aaaccgcaac
     6601 aattccgcga catttatatc ccgatgtccg acaccggcac agcgcaaaaa gcaccaagcc
     6661 cgctgtacga ctggcgaccg atgtccacct acatccgccg tccgccctat tgggaaggcg
     6721 cactggcagg ggaacgtaca ttaagaggta tgcgtccgcc ggcgattttg cccgacaaca
     6781 tcaccaccga ccacatctcg ccatccaatg cgattttggc cggcagtgcc gcaggcgaat
     6841 atttggcgaa aatgggtttg cctgaagaag acttcaactc ttacgcaacc caccgcggcg
     6901 accacttgac cgcccaacgc gcaaccttcg ccaatccgaa actgtttaac gaaatggtga
     6961 gaaacgaaga cggcagcgta cgccaaggtt cgttggcacg cgttgaacca gaaggccaaa
     7021 ccatgcgcat gtgggaagcc atcgaaacct atatgaaccg caaacagccg cttatcatca
     7081 ttgccggtgc ggactatggt caaggctcaa gccgcgaccg ggcggcgaag ggcgtgcggc
     7141 tggcgggtgt ggaagccatc gccgccgaag gtttcgagcg catccaccgc accaacctca
     7201 tcggcatggg cgtcttgccg ctgcaattca aacccggcac caaccgccat accctgcaac
     7261 tggacggtac ggaaacctac gacgttgtcg gcgaacgcac accgcgctgc ggcctgaccc
     7321 tcgtgattca ccgtaaaaac ggagaaaccg tcgaagttcc ggttacctgc cgccccgata
     7381 ctgcagaaga agtattggta tatgaagccg gcggcgtgtt gcaacggttt gcacaggact
     7441 ttttggaagg gaacgcggct tagagttcgt ctgaaaagca agacgtagcg tgggtcgggt
     7501 tctcattcac gtaattcccg atatggcagg catttactgt aaatcgtcat tcccgcgcag
     7561 gcgggaatcc agaaagtgga attgaggaaa cctttttatc cgatgagttt ctgtgcggat
     7621 aaatctggat ttccgcctac gcgggaatga cggggtttaa taatctaccg tatcacaaca
     7681 cagcagccgt agattggggc gaaccccgac agtttgcgga atcaaacggc tttggtcgga
     7741 gtggtagcct aatgtacttc tggaaagtgg gtgtagcgtg ggctttgccc acgaaataaa
     7801 ggctgaattg acatggtata gcggattaac aaa
//
LOCUS       DAXDSD010000091         7812 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXDSD010000091 DAXDSD010000000
VERSION     DAXDSD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.091"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            101..1867
                     /locus_tag="ACFB2Q_001713"
                     /pseudo
     CDS             101..1867
                     /locus_tag="ACFB2Q_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            1971..2639
                     /gene="hda"
                     /locus_tag="ACFB2Q_001714"
     CDS             1971..2639
                     /gene="hda"
                     /locus_tag="ACFB2Q_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC0918691.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            2636..3304
                     /locus_tag="ACFB2Q_001715"
     CDS             2636..3304
                     /locus_tag="ACFB2Q_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC0918692.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(3895..4791)
                     /gene="argB"
                     /locus_tag="ACFB2Q_001716"
     CDS             complement(3895..4791)
                     /gene="argB"
                     /locus_tag="ACFB2Q_001716"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC0918693.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            4961..6091
                     /locus_tag="ACFB2Q_001717"
     CDS             4961..6091
                     /locus_tag="ACFB2Q_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC0918694.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRSDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            6165..7016
                     /gene="lgt"
                     /locus_tag="ACFB2Q_001718"
     CDS             6165..7016
                     /gene="lgt"
                     /locus_tag="ACFB2Q_001718"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC0918695.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(7101..7763)
                     /locus_tag="ACFB2Q_001719"
     CDS             complement(7101..7763)
                     /locus_tag="ACFB2Q_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC0918696.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT         1840 a         2325 c         1877 g         1770 t
ORIGIN      
        1 caagcaatat gccctgaccg gcaaaacttc cccaaacttc atcaaaaaat caactgtacc
       61 cgtctttttc agacggcatt gataccgtaa gagatttccc atgcaaaacc ggcaaaccga
      121 gctttattcc acgccgtctt ggctgttgca gaccttattg atgattaccg ccgcctcggc
      181 ggtgattctg tttttcgcgc gcaacacgcg cttggggcgg gagtttgcct atatcctgcg
      241 cctttgcctg acaccgaaaa gcacggtcaa agtattgctg ctgattacgg caatggttac
      301 gctgctgtta accgaagtgc ggttgaatgt gttgagtacc ttcatgtcca aaggacttta
      361 cgattcgatg caggatttga atgcttcggc attttggatg tttgcagcga tgaacgccgg
      421 cgtggtactg atacgggcgt ttaacaacgt cgttaacgac tttctcgatt aaggcttggc
      481 gattaaatgg tcggaacggc tcaatgaagt tttgacttcg cgctggcttg ccgacaaaaa
      541 ctactaccgc ctgcaaatgc gccgccacgc gccggacaac atcgaccagc gtatccaaca
      601 ggatgcgcag gaattcatcg cttcgaccat cgaatttgtg cgcggcatgg tcaattcggt
      661 cgttacttct ctggaatttg ccgttgtttt gtggggcttg gcaggcatcc tgaccgtgtt
      721 cggcttcgac attccgcacg gcattgtttg gtttgtcttt attttcatga ttttggcgat
      781 ctttatcgcc atgtggatag gcaacccttt gattcgttac aattatgaaa acaaaaaact
      841 caacggcgac taccgttatt ccctcatcct cgtacgcgac cacgccgaaa gcgtggcgtt
      901 ttacagcggc gaacaacacg aacacggccg gctttccgac cgctttaaag ccattatccg
      961 caaccgttgg cgcatcgcgc ggcaaagcgt ctgcttaagc ggctttaacg atatgttcac
     1021 caacggcatc aaactcttcc tgattatttt gcaagctccg cgcctgtttg ccggacaaat
     1081 caaaatcggc gacatccagc agaccgtcca agccttcgcg cgactgcaaa acgcgctgtc
     1141 tttcttccga atgttctaca acaaattcac cgcctgccac gcccgactgg agcgtctgta
     1201 cgtctttttg ctgagtaccg aagaacaaca cggcgcgcag caacccgaga ttagcgaagt
     1261 ttcagacggc attgcactgg aaaacgtcgc cctgttccgc cacaacggcg aagttttatt
     1321 ggacggcatc aacatcaaac tcaaaagcgg cgattccctg ctgatacgcg gcccgagcgg
     1381 tttaggcaaa acctcgctgc tgcgcgcgct ggcggggctt tggccgttcg gcagcagcgg
     1441 caaagtcagc cgtccgccgc atcaaggcat cctcttcctg ccgcaacgcc cgtacacggc
     1501 acaaggcagc ctgcgcgacg cggtttgtta ccccgatatc gacaaacagc atcccgagtt
     1561 agccgaagcc atgaacacct gccgcttggg ttatctgatt gacaaattgg ataaaacaga
     1621 cggctggcag cacaaactct cccccggcga actgcaacgc gtcgccttcg tccgcgccct
     1681 gctctccaag cccaaaatcg tcctgctcga cgaagccgcc gccgccttgg acgaaccggc
     1741 cgaagcctag ctctaccgcg ccttaaagca aaaactgccc gacagcatca tcagtatcgg
     1801 acaccgaagt acgctcaacg cgttccgcaa tatgcacctc gatgtcggca acgctgcctg
     1861 cggctgaacg atgccgtctg aagccgtttt cagacggcat cgttcaaatt ggaaagacac
     1921 gcgaaaacaa cgtacaataa acccgattcc aacccatccg gtatgccttt gtgaaccagc
     1981 ttattttcga ctttgccgca cacgactatc cgagtttcga caaattcctc ggcacggaaa
     2041 atgccgaact ggtctatgtg ttacagcata aacacgaccc gtttatctac gtctggggcg
     2101 aagaaggcgc gggcaaaagc catctgttgc aggcgtgggt cgcacaggcg ctcgaagccg
     2161 gcaaaaacgc cgcctatatc gatgccgcct ccatgccgct gaccgatgcc gcgtttgagg
     2221 cagaatacct cgccgtcgat caagtcgaaa aactgggcaa cgaagaacaa gccctgcttt
     2281 tttccatctt caaccgcttc cgcaacagcg gcaaaggctt tttgctgctc ggttcggaat
     2341 acacgcccca gcagctcgtc atccgcgaag atttgcggac gcgtatggca tactgcctcg
     2401 tttacgaagt caagccacta accgaccaag aaaaaatcga cgcgctcgcc aatatggcgg
     2461 cggcacgcca agtaaccgtc gattccgaaa ttttcgaata cctgctcaaa cactggcggc
     2521 gcgatatgga cagcctgatg atgatgctcg acacgctgga caactacgcc gtaaccatgg
     2581 gcaaacgcat caccctgccg ctgcttcgcc agcttttgaa acaacaggaa acccaatgaa
     2641 aaacctcgcc atcttcgacc tcgacaacac cctcatcaac accgattccg accacgcctg
     2701 gccgcaatac ctcatcaaaa aagggttggt tgacgctgcc gaaaccgaag cgcaaaacga
     2761 aaaattctac cgcgactacc aaaacggctg cctcgacatc gacgcattcc tcaaactcca
     2821 cctcgccccg ctcgcccgtt acagcaaaga agagctggcg gagtttcacc gcgaatttat
     2881 ggcggaatac atcatccccc acatctcgcc tatgcagcgt atgctggtgc agagccacca
     2941 aatggcaggc gacgaaaccc tcgtgatttc ctcaaccaac gagttcatca tcacccccgt
     3001 ctgccgcctt ttcggcatcg ccaacatgat cggcacacaa ctcgaaaccg gtcccgacgg
     3061 ccgctacacc ggcaattaca tcggcacgcc cagcctcaaa gaaggcaaaa tcacccgtct
     3121 gaaccaatgg ctcgccgaac gcggcgaaac gctcgaaagc tacggcaaaa cctattttta
     3181 cagcgactcc aaaaacgacc tgccgctgct gcgcctcgtc agcgaacccg tcgccgtcaa
     3241 ccccgatgcc gaactggaaa aagaagccaa agaaaaaggc tggccggttt tgaatttcaa
     3301 atgacgcaat gccgtctgaa gcccgattcg gcgttcagac ggcattgagg ctgaaatccc
     3361 aaaaacccaa agccacagga attcatccgg aaaaacaaaa aacacaggaa ttcatcggga
     3421 aaaacaacaa tctttccacc gtcattccca cgaaagtggg aatcttgatc cgtccgcacg
     3481 gaaacttata tttcgtcatt ccaccaatcc cacaccacct ttaccggttc aaatccgcac
     3541 aaaaatccac atcccgtcat tcccacgaaa gtgggaatct aggacgtaaa atctaaagaa
     3601 accttttttt cccgataagt ttccgtgccg acaggtctgg attcccgcct gcgcgggaat
     3661 gacgaaattt caaagttatg gcgttatcgg aaaaacagaa aatcaaagcc ggagaattta
     3721 tccaaaacaa ccggatttca aaaaaaccaa attcccggcg ggaatgacgg attttaggtt
     3781 tctgttgtgg tttctgtttt ctcgggaata acaacttaga aattgcccga aaccccaaaa
     3841 acagaaaccg gacaaacagg ttttccgcca aagccggcat tttccgactt tgcttcaggc
     3901 atcttcccct ctgcctaaaa tcatcgaccc gataccggca tcggtaaaga tttccagcaa
     3961 aagcgcgttg ggcaccctgc cgtcgatgat gtgtgtggct ttcacaccgt tggcggcggc
     4021 ttcgaccgca gaagcgattt tcggcagcat accgccatac agcgtgccgt cggcaatcag
     4081 cccatcaatc cgtttcggcg tgagtttggt cagcagattg cccgttttgt ccatcacacc
     4141 ggcgatattc gtcatcatca agagtttttc ggcgttcaat tcttccgcca atttgcctgc
     4201 caccaaatcg gcgttgatgt tgaacgcttc gcctttttca cctacgccga cgggggcgac
     4261 gacgggaatg cagccgcgtt ctatcagccc tttaaccaaa ccggtatcga tgctttccac
     4321 cgtaccgacc tgtccgatgt ccacgctatt ctgttcgggc gtatcgacca aaagtttctt
     4381 cgccttaatg aaatggtcgt cgcgcccgct cacgccgacc gcgtgccctc catatgtgtt
     4441 aatcatcgac acgatttcct tgttgacgtg cccgcccaat accatttcga caatatccat
     4501 cgtctctttg tcggtaacgc gcattccttg gacaaattcg ccctttttgc cgactttttc
     4561 aagcatcgca ttgatctgcg gcccgccgcc gtgaacgatg acgggatgaa tgccgaccag
     4621 cttcagcagc acgacatcgc gggcaaaccc ttctttcaag gcaggttcgg tcatcgcgtt
     4681 gccgccatac ttgatgacgg cgaccgaacc ggaaaaccgg cggatgtaag gcagtgcttc
     4741 ggcaaggata cgcgccttgt cggcggcgga aataatgttt tcaaactcca taacggctcc
     4801 atattgttga caatttcggc ttatgttaac gccaaacgcc gtctgaaaca atcttcagac
     4861 ggcgtttggc acaccttcgg gcaggttgaa gccttgcgat acgggcggta aaatcgcggt
     4921 ttatccggtt cgagaaaccg gcgacagcaa ggactttccc atgattaaaa tcagcacccg
     4981 cttcgatgcc ggctcggtcg tcgtcaaaga cctgaccgac ccttccaaca tccgcctcgc
     5041 cctgcgttcc gacaacgcct ccgatttcgc acaatggttc tacttccggc tgcaaggcgc
     5101 ggcctatcaa aactgcatca tgcattttga aaacgcggca gaagccgcct acccgaaagg
     5161 ctgggaaggc tatcaggcgt gtgcctcata cgaccgccgc aactggttcc gcgtaccgac
     5221 ttcctacgaa aacggcgtac tgaccgtcaa tcatacgccg ctgtccaaca gcgtgtatta
     5281 cgcctatttc gaaccctatt ccgaagagca gcacctcaac ctcctcggcg acgcgcaagg
     5341 cagcggcctg tgccgcatcg acgacttggg cagcaccgtg caaggccgcg acatcaatct
     5401 gctgaccata ggcaaccaag tcgaaagcga catgaaaatc tggattaccg cccgccagca
     5461 cccgggagaa accatggcgg aatggtttgt cgaagggctg ctcggcaggc tgctcgattc
     5521 gcaagacccg acagcacgca ccctgctcga ccgcgccacg ttctacatcg tccccaatat
     5581 gaatcccgac ggttcggcac tgggcaacct tcgtaccaac gccgccggtg cgaacctcaa
     5641 ccgcgaatgg gaaaacccga ctttggaaaa aagccccgaa gtgttcttcg tgcgcggaaa
     5701 aatgctggaa accggcgtgg atttgttttt ggacatccac ggcgatgaag gtctgccctt
     5761 tgtctttgtt gcaggaacgg aaggtgtgcc gaactacaat ccgcgcattt ccgcgttgga
     5821 agcgcagttc aaaaccgccc ttttgaacgc cagccccgat ttccaagacg aatacggtta
     5881 cgaaaaagac gcgccgggca aagcgaatat gaccttggcg accaactggg tcggcaaccg
     5941 tttcaactgc cttgcctaca cgctggaaat gccgttcaaa gacaacgcca acctgcccga
     6001 cgacgacttc ggctggaacg gccagcgttc tttgcggctt ggggaagcgg tgctttccgc
     6061 cattttgaac gtcgccggcg atttgcgctg acactcccca ctgaaaatgc cgtctgaaag
     6121 cccttttgcg tctttcagac ggcatttgaa aacgaaagcc cgttatgatt atccatcacc
     6181 aattcgatcc cgtcctcatc agtatcggcc cgcttgccgt ccgctggtat gccttaagct
     6241 acatcctcgg atttattctt tttacctttc tcggcagaag gcgcatcgcg caaggcttgt
     6301 ccgtttttac caaagaatcg ctcgacgact tcctgacatg gggcattttg ggcgtgattt
     6361 tgggcggacg cttgggctat gtcctgtttt acaaattctc cgactacctc gcccatccgc
     6421 ttgatatttt caaggtatgg gaaggcggaa tgtcgttcca cggcggcttt ttgggtgtag
     6481 ttattgccat atggttgttc agccgcaagc acggcatcgg cttcctcaaa ctgatggaca
     6541 cggtcgcgcc gctcgttccg ctgggtctcg cttcgggacg tatcggcaac tttatcaacg
     6601 gcgaactttg gggacgcatt accgacatta acgcattttg ggcaatgggc ttcccgcaag
     6661 cgcattacga agatgccgaa gccgccgcgc acaatccgct ttgggcagaa tggctgcaac
     6721 aatacggtat gctgccgcgt catccctcgc agctttatca gtttgccctt gaaggcatct
     6781 gcctgttcgc cgtcgtttgg ctgttttcca aaaaaccgcg cccgaccggg cagactgccg
     6841 cgctttttct cggcggctac ggcgtgttcc gctttattgc cgaatttgcg cgccaacccg
     6901 acgactatct cgggctgctg accttggggc tgtcgatggg gcaatggttg agcgtcccga
     6961 tgattgtttt gggtatcgtc ggctttgtcc ggttcggcat gaaaaaacag cactgaccgc
     7021 ctgtgaaaaa taccgtctga aacatggaaa tccggtttca gacggcattt ttacgtttgg
     7081 ttcagatgaa aacaaacgct tcaattctta ccgcaacacg ccttgtattt cctgccgctg
     7141 ccgcacggac agggatcgtt cctgccggtt ttttcccctt ccctgcggac ggtttgcggt
     7201 ttgttgatga ccgcctgcca gtagcggtag atgtctgcca gcgcgtaagg cagttcggac
     7261 gcaagttccg ccagctcgcc ttcggtgaat tgcaggcgga tagcgccgtt ttcctcttcg
     7321 tcgtaaatgc cgcccaatgc catcacgggg taaaacagct cttcaaacgc ttcatcatcg
     7381 gcggcttcaa accaatcggt cggcacaatg tccaaaccgt aaagataggc gttgcaccaa
     7441 gtgtaaaaat cgctgccgcc ctcgccgtcg tcgtagagcc acaaatcggg cagcttttta
     7501 tccgacatcg cggcggttgt ttccatcgcc attgccaaaa ccagccgttc gatttcggaa
     7561 cgttcggcgg cggtaaattg cgattcgtcg cccaacactt cgggcagcca gtcgagcggt
     7621 gccaatttgt ccggcccgct caacagcgcc gtcataaaac cttgaacctc gtcgcaacgc
     7681 atcgtgttgc cttgttcgct tttggcatcc aacaattcgc ccaaccgccg tttggatgct
     7741 tcggtaaatt ttcgggaatc catcattttc cttttcaaat gggttttgcg ccctattata
     7801 gtggattaaa tt
//
LOCUS       DAXDSD010000092         7808 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXDSD010000092 DAXDSD010000000
VERSION     DAXDSD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.092"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            96..1258
                     /gene="prpF"
                     /locus_tag="ACFB2Q_001720"
                     /pseudo
     CDS             96..1258
                     /gene="prpF"
                     /locus_tag="ACFB2Q_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            1570..2769
                     /locus_tag="ACFB2Q_001721"
     CDS             1570..2769
                     /locus_tag="ACFB2Q_001721"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC0918697.1"
                     /translation="MSDQLILVLNCVSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGRRIAHGGEKYHESVL
                     IDQDVLDELKACIPFAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTGMGFTPIEGLVMGTRCGDTDPGVYSYPTFHAGMDVAQVDEMLNEKSGFPGIS
                     ELPNDCRTLEIAADEGREGARLALEVMTCRLAKYIASMAVACGSVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            2880..3500
                     /locus_tag="ACFB2Q_001722"
     CDS             2880..3500
                     /locus_tag="ACFB2Q_001722"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC0918698.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIINQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDAPARALHPKRKTIGLRIPDNAVAQALLEELGGPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTDPTTVIDMTDGTELVRRGFGDTAVFGL"
     gene            complement(3524..3652)
                     /locus_tag="ACFB2Q_001723"
     CDS             complement(3524..3652)
                     /locus_tag="ACFB2Q_001723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918699.1"
                     /translation="MPPKPAFGRHFVCRFKRIFRPGAIRAGISGCRRQGARCRLNG"
     gene            complement(3662..4105)
                     /locus_tag="ACFB2Q_001724"
     CDS             complement(3662..4105)
                     /locus_tag="ACFB2Q_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC0918700.1"
                     /translation="MYLTQHTDYGLRVLIYTAVNDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYESKIPIAVR"
     gene            4279..4647
                     /locus_tag="ACFB2Q_001725"
     CDS             4279..4647
                     /locus_tag="ACFB2Q_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC0918701.1"
                     /translation="MKPLKRHSALVGLSREHHHSLSLCVRMLRTPGADHRAELELHFA
                     ELETHFREEEAKFAPIWQNVAPELKTRFEGDHAKLRQMMASPECGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            4692..5846
                     /locus_tag="ACFB2Q_001726"
     CDS             4692..5846
                     /locus_tag="ACFB2Q_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC0918702.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTAFWLAARIAAFIPGWG
                     AAASGILGTLFFWYGAVCMALPVIRSQNRRNYVAVFAIFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGMRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAIL
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNSIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(5904..6776)
                     /locus_tag="ACFB2Q_001727"
     CDS             complement(5904..6776)
                     /locus_tag="ACFB2Q_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC0918703.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVINRASVVIDADSIGGADLVLIATPVATVPAVLTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRNGAQAAQFGLFRHRKLIITPHGGEDSDGI
                     ALVENLWRAVGADIFTMDAQHHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            6861..7673
                     /locus_tag="ACFB2Q_001728"
     CDS             6861..7673
                     /locus_tag="ACFB2Q_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC0918704.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTGKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGCDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGREVPHLSAEGMPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKHRVLDAV"
BASE COUNT         1659 a         2217 c         2271 g         1661 t
ORIGIN      
        1 ccctttgagc cggggcgggg caacccgtac cggtttaaat taatccacta taaaaatcgt
       61 gggcagagcc cacgctacat aaggagaacc ctaaaatgcc gcaaatcaaa atccccgccg
      121 tttactaccg cggcggtaca tctaaaggca ttttcttcaa acgcaccgat ctgccggcag
      181 ctgcgcagga agcaggcggg gcacgcgaca aaatcctctt gcgcgtactc ggcagccccg
      241 acccttacgg caaacagatt gacggtttgg gcaacgccag ttcgtccacc agcaaagctg
      301 tgatttcgga caaatccgaa cgcgccgatc acgatgtcga ttacttgttc ggacaagttt
      361 ccatcgacaa accatttgtc gattggagcg gcaactgcgg caaccttacc gccgccgtgg
      421 gcacattcgc catcgaacag ggcttggtcg ataaatccaa aatcccttca gacggcatct
      481 gcacggtcaa aatctggcag aaaaacatcg gcaaaaccat tattgcccat gtgccgatgc
      541 aaaacggagc agtgttggaa acaggcgatt tcgagcttga cggcgtaatt ttccggcagc
      601 cgaagtacaa atcgaatttt tagatccagc cgacggcgaa ggcagtatgt tcccaaccgg
      661 caatttggtc gacgaaattg atgtgccgaa tataggtcgt ctgaaagcca cgctcatcaa
      721 cgcgggcatt ccgaccgttt tcctgaacgc cgccgacttg ggctacacgg gcaaagagtt
      781 gcaggacgac atcaacaacg atgccgccgc gctggaaaaa tttgaaacca tccgcgcata
      841 tggcgcgctg aaaatgggtt tgatcagcga cgtatccgaa gccgccgccc gcgcgcgcac
      901 gccgaaaccc gccttcgtcg cgcccgccgc cgattacacc gcctccagcg gcaaaaccgt
      961 aaacgccgcc gacatcgatt tgccggtacg cgccctgagc atgggcaaac tgcaccacgc
     1021 tatgatgggc atcgcctcgg tcgccatcgc cgccgccgtg ctcggtacgc tggtcaacct
     1081 tgccgcaggc ggcggaacgc gtaaagaagt gcgcttcggg catccgtcag gtacgctgcg
     1141 tgtcggtgct gccgccgaat gtcaggacgg acaatggacg gccgccaaag cggtcatgag
     1201 ccgcagcgca cgcgtgatta tggaaggttg ggtgcgcgtt cccgatgatt gtttttgaaa
     1261 tcatgtagga cgcacattgc tgtccgctgt ttcagacggc atttgattaa ggattaccat
     1321 aaagccgaat aacctcaccg ccgtcattcc cacgcaggcg ggaatccgca gccgatattc
     1381 tgtttcctga tttttatcag agcctgcgtg tgagcaaata tggattcccg cctgcgtggg
     1441 aatgacgcta ggaaatacgg catacgcttt gcccaaagag gccgtctgaa acacactccg
     1501 cccaacgccc atcctttttc agacggcccc gcaccaaaaa aacaaccaca aactacaagg
     1561 agaaacatca tgtccgacca actcattctt gtcctgaact gcgtcagttc atcgctcaaa
     1621 ggcgccgtta tcgaccgcaa aagcggcagc gtcgtcctaa gctgcctcgg ggaacgcctg
     1681 actacgcccg aagccgtcat taccttcaac aaagacggca acaaacgcca agttcccctg
     1741 agcggccgca actgccacgc cggcgcggtg ggtatgctgt tgaacgaact ggaaaaacac
     1801 ggactgcacg accgcatcaa agccatcggc cgccgcatcg cccacggcgg cgaaaaatat
     1861 cacgagtccg tcctcatcga ccaagacgtc cttgacgaac tgaaagcctg catcccgttc
     1921 gccccgctgc acaaccccgc caacattagc ggcatcctcg ccgcgcagga acactttccc
     1981 ggcctgccca acgtcggcgt gatggacacc tcgttccacc aaaccatgcc ggagcgggcc
     2041 tacacttatg ccgtgccgcg cgaattgcgc aaaaaatacg ccttccgccg ctacggtttc
     2101 cacggtaccg gtatgcgtta cgtcgcccct gaagccgcac gcatcttggg caaacctctg
     2161 gaagacatcc gcatgattat tgcccactta ggcaacggcg catctattac cgccgtcaaa
     2221 aacggcaaat ccgtcgatac cggtatgggt ttcacgccga tcgaaggttt ggtaatgggt
     2281 acacgttgcg gcgacaccga tccgggcgta tacagctatc cgactttcca cgcagggatg
     2341 gatgttgccc aagttgatga aatgctgaac gaaaaatcag gtttccccgg tatttccgaa
     2401 cttcccaacg actgccgcac cctcgaaatc gccgccgacg aaggccgcga aggcgcgcgc
     2461 ctcgccctcg aagtcatgac ctgccgcctc gccaaataca tcgcttcgat ggctgtggcc
     2521 tgcggcagtg ttgacgcact cgtgttcacc ggcggtatcg gcgaaaactc gcgtaatatc
     2581 cgtgccaaaa ccgtttccta tcttgatttc ttgggtttgc acatcgacac caaagccaat
     2641 atggaaaaac gctacggcaa ttcgggcatt atcagcccga ccgattcttc tccggctgtt
     2701 ttggtcgtcc cgaccaatga agaactgatg attgcctgcg acactgccga acttgccggc
     2761 atcttgtagc caaaaaaggg ggcgagtccg caaaaatgcc gtctgaaagg ttttcagacg
     2821 gcattttgtt tggcgtttgg gcgtacaata cggaaaacat gacgataagg aaacaaacta
     2881 tggcacaatt tttcgccgtt catcccgaca atccccaaga acgcctcatc aagcaggcag
     2941 tcgaaatcat caatcaaggc ggcgtggtgg tttatccgac cgattcctgt tacgccttgg
     3001 gttgcaaact cggcgataag gcggcgatgg aacgcatact ctccatccga aaaatcgatt
     3061 tgaaacacca cctgaccctg atgtgcgccg atttgagcga gttgggcaca tacgccaaaa
     3121 tcgacaacgt acagtttcgt cagcttaaag ccgccacgcc cgggccttat acctttatct
     3181 tgcaggcgac gaaggatgcg ccggcgcgcg ccctgcaccc gaaacgcaaa accatcgggc
     3241 tgcgtattcc cgataatgcc gttgcacaag ccctgctgga ggagttgggc gggccgcttt
     3301 taagctgcac cctgatgctg cccgaagacg gcgaaccatt gaccgaccct tatgaaatcc
     3361 gcgagcgttt ggaacacgcc gtcgatttgg taattgacgg cggctggtgc ggaaccgatc
     3421 cgactaccgt catcgacatg accgacggca cggaactggt gcgccggggt ttcggcgata
     3481 cggcggtgtt cggtttgtag ggaaaccgat gccgtctgaa gcatcagccg ttcagacggc
     3541 atcgcgcgcc ttgccggcgg cagcccgaaa tgccggcgcg tatcgcgccc ggtcggaata
     3601 tccgtttgaa acggcaaaca aaatgccgtc cgaacgcggg tttgggcggc attttgatgc
     3661 gttatcgcac cgcaatcggg attttcgatt cataaagcag gtcgtaggtc ggcttgttga
     3721 gcaggtcttg cagcgtgaaa ccgtccagat acgtgaaaaa cgacttcatt gcaccgccga
     3781 gtatgcccgt cagccggcag gacggcgtaa tcaggcattc gttgttcggg cccatacact
     3841 cgaccagctg catcggttcg aggtggcgga cgaccgcgcc gatattgatg cgttcgggcg
     3901 gcgcggccag cctcagcccg ccgcctttcc cgcgtacgct gtgcaagaac ccgcctttga
     3961 ccagcgcggt aaccactttc atcaaatggc ttttggaaat gccgtaggtc gaggcgatgg
     4021 tggcgatatt gaccagcgcg tcgtcgttga cggcggtgta gatgaggacg cgcagcccgt
     4081 agtcggtatg ttgggtcaga tacatgattt tctcggtatg gatcgttatt cttatcggta
     4141 cggtttaagt ttcacggaaa ataccttaat ggttgaaacc ctgtccgtcg gggcggtaga
     4201 atgtagcctg tctgcggcgg tatgccgtct gaaacatccg cgctaccgtt tgataatttg
     4261 ttattgtaac ctaaatttat gaaaccgttg aaacgacatt ccgcccttgt cgggctttcg
     4321 cgcgaacacc accactcgct ttccctgtgc gtgcgtatgt tgcggacgcc cggggcagac
     4381 catcgtgccg aactcgagct gcattttgcc gaactggaaa cccattttcg cgaagaagaa
     4441 gccaagtttg ccccaatttg gcagaatgtc gcgcctgagt tgaaaacccg ttttgaaggc
     4501 gatcacgcca aactgcggca gatgatggca agccccgaat gcggtaacgc ggcgtggaat
     4561 accgcttttg ccacaaccct gcgcgaccac gcgcgctttg aagaacgcga gctgtttccc
     4621 gccgtcgaac cgtttttgcc ggcatgattc cgttttgcgg caaatatatt aatgataaac
     4681 aaggaacaca catgaaattt accaaacatc ccgtctgggc aatggcgttc cgcccgtttt
     4741 attcactggc ggcactgtac ggcgcattgt ccgtattgct gtggggtttc ggctacacgg
     4801 gaacgcacga gctgtccggt ttctattggc acgcgcatga gatgatttgg ggttatgccg
     4861 gtctcgtcgt catcgccttc ctgctgaccg ccgtcgccac ttggacggga cagccgccca
     4921 cgaggggcgg cgttctggtc ggcttgaccg ccttttggct ggctgcgcgg attgccgcct
     4981 ttatcccggg ttggggtgcg gcggcaagcg gcatactcgg tacgctgttt ttctggtacg
     5041 gcgcggtgtg catggctttg cccgttatcc gttcgcaaaa ccggcgcaac tatgtcgccg
     5101 tattcgcaat atttgtgctg ggcggtacgc atgcggcgtt ccacgtccag ctgcacaacg
     5161 gcaacctagg cggactcttg agcggattgc agtcgggcct ggttatggtg tcgggcttta
     5221 tcggcctgat tgggatgagg attatttcgt tttttacgtc caaacggttg aacgtgccgc
     5281 agattcccag tccgaaatgg gtggcgcagg cttcgctgtg gctacccatg ctgaccgcca
     5341 tactgatggc gcacggcgtg atgccttggc tgtcggcggc tttcgcgttt gcggcgggcg
     5401 tgatttttac cgtacaggtg taccgctggt ggtataaacc cgtattgaaa gaaccgatgc
     5461 tgtggattct gtttgccggc tatctgttta ccggattggg gctgattgcg gtcggcgcgt
     5521 cttatttcaa acctgccttc ctcaatctgg gcgtacatct gatcggggtc ggcggtatcg
     5581 gcgtgctgac tttgggcatg atggcgcgta ccgcgctcgg tcatacgggc aattcgattt
     5641 atccgccgcc caaagccgtt cccgttgcgt tttggctgat gatggcggca accgccgtcc
     5701 gtatggttgc cgtattttct tccggcactg cctacacgca cagcatccgc acgtcttcgg
     5761 ttttgtttgc actcgcgctg ctggtgtatg cgtggaaata cattccgtgg ctgatccgtc
     5821 cgcgttcgga cggcaggccc ggttgagtaa aaccgccgca gatttcgggt ctgcggcggt
     5881 ttgcttttca gacggcatgg cggtcagttg ccgtccagcc agcggtcgcg cgtggttttg
     5941 gcttcttcaa aatagcggta cagggcttcg cggtcgtcgg cggtcaggat gtctgccaaa
     6001 acgtccaact gtttgcccaa gccttgaacc agttgcagca ggctgtcttt gttggcaagg
     6061 cagatgtccg cccacacggc gggatggccg gaggcgatgc gggtgaagtc ccgaaagccc
     6121 gtggcggcga atttcagata ttcctgcccg tcggggtggt cgagaatctg gtggacatag
     6181 gcgaaggcgg tcaggtgggg catatgggag acggcggcga aaaccgcgtc gtggtgttgc
     6241 gcgtccatcg tgaaaatgtc cgcaccgacc gcgcgccaca ggttttctac caatgcgatg
     6301 ccgtctgaat cttcgccgcc gtggggcgtg atgatgagtt ttctgtggcg gaacagcccg
     6361 aactgcgcgg cttgcgcacc gtttctgtcc gaaccggcaa tcgggtgtgc ggcgatgcag
     6421 cggtgcaggc ggtcgggcag gcagcggcgg aaggcttcga tgaccgaaga tttggtgctg
     6481 ccgacatcgg aaatccaagt gtgttccggc aaaacggggc gcagcgcggt taaaacggcg
     6541 ggaacggtgg cgacgggcgt ggcaatcagt accaagtccg caccgccgat gctgtccgcg
     6601 tcgatgacga cggaagcccg gttaatcacg ccgcgttcca atgcacgttc gaggttgtcg
     6661 cggtcggtgt cgataccggt aacggtgcgg acgagtccct gccttttgag gtcgagaacg
     6721 aacgaaccgc cgatcagccc tacaccgatg agggcaatat ggttcaaaat gggcatttgt
     6781 gtaaacggtt ttcgcaaagt gccgccatgg tagcctatcg gcggaatatg ccgcaaggtc
     6841 ggcaggaaaa aggagaagaa atggacaaaa tcagagttgc cgccgtgcag atggtgtcgg
     6901 gcgtgtcgcc ggaaaccaac gtcgccgcca tgaaacgcct ggtcgcacgg gcggcggagc
     6961 agggtgcgga ttgggtgctg ctgcccgaat attgggtgct gatgggcgca aacgataccg
     7021 gcaaactcgc acttgccgag cctttgggcg gcggacgctt tcagacggca ttgagcgaaa
     7081 cggcgaaaga atgcggcgtg gtgctgttcg gcggaaccgt gccgctgcaa agccccgaag
     7141 cgggcaaggt catgaatact ttattggtgt acgggtgcga cggcgtaagg acggggctgt
     7201 accacaaaat gcacctcttc ggtttttccg gtttgggcga acgctatgcc gaagccgata
     7261 ccatccgcgc agggcgggaa gtgccgcact tgtcggcaga aggcatgccg gtggcggcag
     7321 gtatttgtta cgatgtccgc tttcccgaat ttttccgccg ccaactgccg tttgacgtat
     7381 tgatgctgcc ggcggcattt acccatacga cgggcaaggc gcactgggag ctgctgctgc
     7441 gcgcgcgtgc cgtcgaaaac caatgttacg tcgtggcggc ggcacagggc ggtttgcacg
     7501 aaaacggacg gcgcacgttc ggacacagta tgattgtcga tccgtggggc gacgtgttgg
     7561 acgtattacc cgaaggcgaa ggcatcgtta cggcagacat cgatgccaac cgcctgaaca
     7621 gcgtccgcaa ccgcctgccc gccttgaaac accgggtttt ggatgccgtc tgaaagttca
     7681 gacggcatcg gtgtcgggga atcagcgggc cgtagcaacc gaaccgtcat tcccacggaa
     7741 gtgggaatct agaatctcgg ggtttcagtc atttccgata gattcccgcc gcgtcggggg
     7801 tccggatt
//
LOCUS       DAXDSD010000093         7793 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXDSD010000093 DAXDSD010000000
VERSION     DAXDSD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.093"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            357..803
                     /gene="yciA"
                     /locus_tag="ACFB2Q_001729"
     CDS             357..803
                     /gene="yciA"
                     /locus_tag="ACFB2Q_001729"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC0918705.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLATP"
     gene            complement(854..1675)
                     /locus_tag="ACFB2Q_001730"
     CDS             complement(854..1675)
                     /locus_tag="ACFB2Q_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC0918706.1"
                     /translation="MAVLITGASAGFGEAMCRTFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSCRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERVAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1794..2252
                     /locus_tag="ACFB2Q_001731"
     CDS             1794..2252
                     /locus_tag="ACFB2Q_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC0918707.1"
                     /translation="MKTQISLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2395..3531)
                     /gene="lst"
                     /locus_tag="ACFB2Q_001732"
     CDS             complement(2395..3531)
                     /gene="lst"
                     /locus_tag="ACFB2Q_001732"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC0918708.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVRRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTNNLIRESSYLGDEFYVNGAIKRNFARMMVGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGGETGGTVRILLGSPDKEMKEISQKAAKNFNIQY
                     VAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFRQADIPILAFDDKNQSHGKSK"
     gene            complement(3522..3782)
                     /locus_tag="ACFB2Q_001733"
     CDS             complement(3522..3782)
                     /locus_tag="ACFB2Q_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918709.1"
                     /translation="MAALFALCSILACLNAAAGKEGRNGFPAFGGGVGGVVPDVRRHI
                     FGKIDFNIRQLSEYLLKFRIFRTGTLGAYVQFVGMEFLGIWG"
     gene            3861..6086
                     /locus_tag="ACFB2Q_001734"
     CDS             3861..6086
                     /locus_tag="ACFB2Q_001734"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC0918710.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEITAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGGIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQVLNA
                     L"
     gene            6383..6685
                     /locus_tag="ACFB2Q_001735"
     CDS             6383..6685
                     /locus_tag="ACFB2Q_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918711.1"
                     /translation="MKTVPTILTGILLATALPASAHGMHKSKPLAMDELPPICQQYFK
                     RTETCYNKAGNKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRNLNCE"
BASE COUNT         2130 a         2218 c         1725 g         1720 t
ORIGIN      
        1 aattcccgcc attttccatc attcccgaca acaccgcaat ctcgaaaccc gtcattcccg
       61 cgcaggcggg aatctaggat gcggaatcta gagaaacctt ttaaccgata agtttccgtg
      121 cggacaggtc tggattcccg cctgcgcggg aatgacggtt cggttgctta gggtaggatt
      181 tggcgggatt ggtgggctga agcccgccct acgcatccgc cctacaatac tccttgcgaa
      241 tctgtcccgc gcccgacctt gtatgtctca aatgggttaa actacgcact tcccgacttc
      301 ccgtttcggg caggggtgca ggtccgattt atatttcaat aaaacaagga aactttatgc
      361 agcacgaaga aggcaaccgc caacgccctc aaggcgaact gctcctgcgt accgtcgcta
      421 tgccgcgcga taccaatccc aaccaagaca ttttcggcgg ctggattatg tcgcaaatgg
      481 atttgggcgg cggcatattg gcggcggaaa tcgcgcgggg gcgcatcgtt accgtcgccg
      541 ttcaggaaat gaacttcatc cgcccggtca aggtcggcaa cgtcgtctgc tgctacgggc
      601 attgcgtccg cgtgggcaat acttccctcc agcttaaagt cgaagtctgg gtgaaaactt
      661 tgatgaacga ttgcgttacc gaagaccgcc atctcgtaac cgaggcggtg ttcacttatg
      721 tcgccatcga tgcggaaggc aatccgcgcc cgattccgaa agaaggcaac cccaaattgt
      781 caggcttatt ggccaccccg taagaatacc cgtaaaaatg ccgtctgaaa ctgtcttcag
      841 acggcattcc gtatcatttg aaccaagacc tgatgcggga aaacagcgac atactctgtt
      901 tttcaaagtc ttcccgcact tcgggcacgg ctgtttttat gaccttcatt cctgcaaaag
      961 tctgcgccac aggcataatt tcaatcgtgt tcacattcat atgcgccggc cggcggtaca
     1021 gccacaatgc ggtttccgca atatcttcgg ggcggataaa ttccacaccc tcatacacgc
     1081 ccgccaccct ctcgttatcg cctttgaagc gcacgttgga aaactccgta ttgccgcaca
     1141 aacccggctc gatattggta acgcggatgt tcttatccgc cagctctgcg cgcaaattca
     1201 ggctaaactg gcgcacaaac gccttggtcg ccccgtaaac gttgctgccg gcataagcat
     1261 aattgcctgc aatcgaaccc aaattcatca cataaccgcc gccgcgttcc accatttgcg
     1321 gcaaaatctt gcgcgtcagg aacgtcaaac ccaaaacatt ggtttgaatc attgtttccc
     1381 aatcttcaaa atccgccttg tccgccgtgt ccaaacccaa agccagcccg gcattgttga
     1441 tgaggcagtc gatgtcggaa aattcatcgg ggatgccgtt caaggcgttt tccaccgact
     1501 cgcggcacga cacgtccatt tccaaagggt aaaacaaagc acccaattca tccgccaaag
     1561 cctgaagcct gtccgcacgg cgcgccgcac cgataacgcg gtatcccgcc ccgacaaacg
     1621 tgcggcacat cgcttcgccg aagcctgccg aagcaccggt aattaaaaca gccattgttt
     1681 ttcctttctt tctcgtttcc caaacctgtt tcaggtatca taacaccctt cagacggcat
     1741 cgcgccggct gaaaacgaca acaaccgtac caccacgaca ggacacattc aacatgaaaa
     1801 cccaaatcag ccttgccgca accgccctcg ccctattcct ttccgcctgc ggaaacggcg
     1861 gcgcgcccgc acagcccaaa ggcgaaattt ccgaaaaccg caccgccgcg ttcaaatcta
     1921 tgatgcccga cttctcgcgt atgggcaaaa tggtcaaagg cgaagagcct tacgatgtcg
     1981 aaaaattcaa acaggcggcg gcggcgtttg ccgaaagcag caaaaaaccg ttcacacttt
     2041 ttgagtccga tccgcaaggc aacggccgcg ccctgcctgc cgtttggtcg gatggtgcaa
     2101 aatttgaagc cgaaaaaaca aaattcgccg ccgccgtcga aaaactcaac gccgccgccc
     2161 aaaccggcaa actggacgaa atcaaagccg cctacggcga aaccggcgca agctgcaaat
     2221 cctgccacga cagtttccgc gcgcccgaat aacatccgaa acagaacaat gccgtctgaa
     2281 tccttcagac ggcattttct ttgtcatttc gccacgcgca aagcaatcgg aaattatctt
     2341 caattcaaaa aatacgccat atcctgattt ttgccttact tactccgcca ttttctattt
     2401 tgatttacca tgcgattgat ttttatcgtc aaatgccaaa atcggaatgt cggcctgacg
     2461 gaacagcgca taaacgggct tgagccaata atcttccgga agggaagccg gtttcaatgc
     2521 gtaaacgtgc acattgggaa aatccttcat cgtcaacgcc gcaccgctga aaaaggtata
     2581 aatttcatac ctcgtatgcg ggtttttctt aatttcgcgc aaaatatagt cttcgatgac
     2641 atagggcgaa tttaacgcgg ttacgccgga aagcccgtag gtctggcggg gatgcggcgc
     2701 gacatattgt atgttgaagt tttttgccgc cttttgcgaa atttccttca tctccttgtc
     2761 gggcgaaccc aaaagtatcc gcaccgtgcc gcccgtttcg ccccccgcct tcagttcgga
     2821 cgcatcgaac agcggcaggt aagtcatctt gcggcggccg tcgtccataa tgtttttcaa
     2881 acccttgaat atcgtgtaat gctcgtcgga agcattgcgg gttttggcga tgctccaatc
     2941 cccgaccatc atccgggcaa aattccgctt aatcgccccg tttacataaa actcatcgcc
     3001 caaatagctg ctctcccgta tcaggttgtt tgtgccgtcg tcaaaggtct tgatttccgc
     3061 atccgggtaa gtactcaaaa aggcggcaat actgactttt tccaaactcg ccaaataaat
     3121 ccgcctgacc ttcggcagca gcatcgactt caccttcagc tccgccatcg tcggaatgaa
     3181 attaaacgat ttgttcaaac cgtagggcag gtagaaaaaa tacgcccgct ccgccttatc
     3241 ctttatctga ttgaaataat aatcgtattt ttcattcctg ttttcagaca tcagcaccac
     3301 ataaaaccgc tcccccggat gctgcgccat aatcctttct gccaccttca tctgcaatat
     3361 ggtatagcag aaaatcagat tgacgggttt cccctcttca ttgaagagtt tgtccttcag
     3421 cagggaaacc gcgttccttt ccccctgatt tacccggtca aacgtataaa atatcccgaa
     3481 gcaaaaaaca atcaggcaca acacggtcaa acagactttt ttcaacccca tatccctaaa
     3541 aactccattc cgacaaattg aacatacgcc ccgagtgtcc cggtgcggaa aatgcggaat
     3601 tttagcagat attccgacag ttgccgaata ttaaaatcaa ttttaccgaa aatgtgccgc
     3661 cgcacatcag gcacaacgcc gccgacgccg ccgccaaacg cgggaaagcc atttctccct
     3721 tcctttcccg ccgccgcgtt caaacacgcc aagatcgaac aaagcgcgaa caaagccgcc
     3781 aaaatatgcc ataatagccg cgcacattcc ccgcgcaatg tgtttcaaaa gaaaaaacca
     3841 acctacaaga gaataatcct atgactcaaa aatccaccat tgtttatacc cataccgacg
     3901 aagcccccgc gctggcgacc caatcgctgc tgccgattgt gcaggcgttt gcccgccacg
     3961 ccgatattga tgtcaaaact gccgacattt ccctgtccgg ccgcattttg gcggcattcc
     4021 ccgaatatct gaccgaagcg cagcgcgtac ccgacgcgct tgccgaattg ggcgaactgg
     4081 tgaaacaacc cggtgcaaac gtaatcaaac tgccgaacat cagcgcatcc gtacctcaac
     4141 tgaccgccgc gattaaagaa ttgcagtcta aaggctttgc cgttcccgac tatcctgccg
     4201 accctcaaac cgatgaagaa aaagccgtac gcgaacgcta cgaccgcatc aaaggcagcg
     4261 cggtaaaccc tgtcctgcgc gaaggcaact ccgaccgccg cgcgcctaaa gcggtgaaaa
     4321 actttgccaa aaaaaatccg cacagcatgg gcgcgtgggc gaaagactcc aaaacccacg
     4381 ttgccaccat gcaaagcggc gactttttcc ataacgaaca atccgttacc gtacccgatg
     4441 cgacttccgt atccatcgtg ttcaccgaca aacaaggcaa taaaaaagag ctgcgcgagc
     4501 ctgttgccct gaaagccggc gaaatcattg acgcggccgt aatgagcaaa aaagccctgc
     4561 tcgccttcct tgccgaacaa gtgaaagacg caaaagctaa aggcgtattg ttctcgctgc
     4621 atatgaaagc cacgatgatg aaagtgtccg acccgattat cttcggacac gccgtcaaag
     4681 tgttcttcgc gcctgtattt gaaaaattcg gcggcaaact ggctgccgca ggcgtcaacg
     4741 tcaacaacgg cttcggcaac ctgattgcca atctggacaa actggatgcg gacacccgcg
     4801 ccgccgtcga agctgaaatc accgccgttt acgccgccaa cccggatttg gcgatggtcg
     4861 attccgacaa aggcatcacc aacctgcacg ttcccagcga tgtcatcgtt gatgcttcta
     4921 tgcctgcgat gatccgcaat tccggccgta tgtgggacaa aaacggcaaa gcgcaagaca
     4981 ccaaagccgt gattcccgac agcagctatg ccggtgttta ccaagcaacc atcgatttct
     5041 gccgcgaaca cggcgcattc gatccgacaa caatgggtac tgtgcccaac gtcggactga
     5101 tggcgcaagc ggcggaagaa tacggctcac acaacaaaac ctttgaaatc gaagccgacg
     5161 gtcaggttca agtgattgat gcggcgggca atgtcctgat gcagcacgac gttgaggcag
     5221 gcggcatctg gcgcatgtgc caaaccaaag acgctcccgt caaagactgg gtacaactcg
     5281 ccgtcaaccg cgcccgcctg agcaacacgc ccgccgtgtt ctggctcgac gaaaaccgtc
     5341 cgcacgacaa gagtctgctc gccaaagtca aagcctacct tgccgaattg gataccaacg
     5401 gcctcgacat ccgcgtcctc gcgcccgaag aagccgctaa attcagcttg ggccgtctga
     5461 aaaacggcga agacaccatt tccgtaaccg gcaacgtcct gcgcgattat ctgaccgact
     5521 tgttcccgat tttggaactg ggtaccagcg cgaaaatgct gtccatcgtt ccattgatga
     5581 acggcggcgg tatgtttgaa accggcgcgg gcggttctgc accgaaacac gtccaacagt
     5641 tcctcgaaga aaaccacctg cgttgggact cattgggcga attcctcgcg cttgccgtat
     5701 cgtttgaaca tctggcgcaa aaaaccggca acgccaaagc ccaagtcctc gccgacactt
     5761 tggatgccgc caccgaaaaa ctgctgttga acgacaaatc gcccaaacgc aaagcgggcg
     5821 aactcgacaa ccgcggcagc catttctacc tcaccctcta ctgggcgcaa gaattggcgg
     5881 cgcaagacaa agatgccgaa ctgaaagccg catttgctcc attggcagcc gctttgactg
     5941 ccgacgaagc caaaatcgtt gaagaactct ctgccgtaca aggcaaagcg gtcgacatcg
     6001 gcggctacta cgcagccaat cctgaaaaag ccgcacaagc gatgcgtccg agcgcaacct
     6061 ttaatcaagt actaaacgcc ttataagcac aaaggcaaaa atgccgtctg aacattcgtc
     6121 gttcagacgg cattagttat ccctatctgc ctgattatga ttaaacgccg cacccattgg
     6181 atatatagtg gattaacaaa aaccagtacg gcgttgcccc gccttagctc aaagagaacg
     6241 attctctaag gtgctgaagc accaagcgaa ccggttccgt actatttgta ctgtctgcgg
     6301 cttcgtcacc ttgtcctgat ttttgttaat ccactataaa tcatataatc tttagttttt
     6361 taccctacgg aaagacaata atatgaaaac tgttccaacc atcctgaccg gcatcctttt
     6421 ggcaaccgcc cttcccgcat ccgcacacgg tatgcataag agcaaacccc tggctatgga
     6481 cgagctgccg ccgatttgcc aacaatattt caaacgcacc gaaacctgtt acaacaaagc
     6541 cggaaacaaa gcagatttcg cacgcaacaa caccaaattc ctattccaag ccctacctgc
     6601 cgccgatttg ggacagcgta agcaaatgtg ccaaatcgct atggattcgt ttgcagaaaa
     6661 aacccgcaat ctgaattgcg aataaagccg catcagcaat agcaatgccg tctgaaacaa
     6721 aggttcagac ggcattctca catcaaaacg taccggctga taaaacccgc cttccccgca
     6781 tccctgaccc gaacaggatt aaacggagta acggtttcgg ccattatggc aagtccccat
     6841 cccaaaaatg accatataaa ttaataccat aaaacattga taaaaagcgt ctgccggttg
     6901 cggtttgttg cagggcgtta cggaaaatca tcgtaaaccg atgattttta tatagtaatt
     6961 gacacaacct gaacaaaaaa gaaccgccct gaatcagggc ggttttgctt tgcggcggaa
     7021 acggcaggat tcgactaaat tttattttat tgatttaaat acatttattt cttataaaaa
     7081 tttaatttac cataaaaaca gccatataca aaaatcttgg agtaactact gcattacact
     7141 attagaagga tgcagggtca tgtaatattt aattaaatta atatataaat taaatatttg
     7201 aattatattt ataaaaaaag gtgcaaataa ggtgcaaata aaattagttt ttcccaatgg
     7261 gttaaagaac aaatccgaaa agagttctta aaatagcccg accgccgcca agccgcaaac
     7321 ttggcggctt ctttatgccg tccgaaagcc cttcagacgg catttttctt tgattttgtt
     7381 gggggaatgg ttgactttga acgtgtccac gtttataatt caattcatcg gcgcatcggg
     7441 tgccgatacg ggaaaagccc caccgaagcg gggcttgtac agggagtagg aaaatgaagc
     7501 tcatccttca aattctcatc ctggttttaa tcttgatgct ggcaggcgat acgcctatta
     7561 gcggataaaa ccggctgggg cggtagccgc cgccccagtc cctatcttac aaaaggcagt
     7621 tcaaaaaatc aaggagtttg cctatggtta atgaaaaact ggcagaaaac cgaaagcgtt
     7681 acgagcaaaa acgagtcatt aaaaaagtct cgtttaatgc ggaaacagaa aaagagcttt
     7741 tggaatatgc ccaaaatctc gatttttccc aatgggttaa atcaatcata aaa
//
LOCUS       DAXDSD010000094         7570 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXDSD010000094 DAXDSD010000000
VERSION     DAXDSD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.094"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(12..428)
                     /gene="dksA"
                     /locus_tag="ACFB2Q_001736"
     CDS             complement(12..428)
                     /gene="dksA"
                     /locus_tag="ACFB2Q_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC0918712.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
     gene            complement(577..1386)
                     /gene="proC"
                     /locus_tag="ACFB2Q_001737"
     CDS             complement(577..1386)
                     /gene="proC"
                     /locus_tag="ACFB2Q_001737"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC0918713.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLGKELGVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTL
                     SRYLGGSRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            complement(1417..1824)
                     /locus_tag="ACFB2Q_001738"
     CDS             complement(1417..1824)
                     /locus_tag="ACFB2Q_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC0918714.1"
                     /translation="MEIRVIKYTATAALFAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1824..2510)
                     /locus_tag="ACFB2Q_001739"
     CDS             complement(1824..2510)
                     /locus_tag="ACFB2Q_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC0918715.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2630..3673
                     /gene="pilT"
                     /locus_tag="ACFB2Q_001740"
     CDS             2630..3673
                     /gene="pilT"
                     /locus_tag="ACFB2Q_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC0918716.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            3837..5063
                     /locus_tag="ACFB2Q_001741"
     CDS             3837..5063
                     /locus_tag="ACFB2Q_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC0918717.1"
                     /translation="MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5180..7330
                     /gene="yccS"
                     /locus_tag="ACFB2Q_001742"
     CDS             5180..7330
                     /gene="yccS"
                     /locus_tag="ACFB2Q_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC0918718.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1826 a         2544 c         1673 g         1527 t
ORIGIN      
        1 gtttgcctcc atcagtcggc aaactgtttc ttcatcctct ctcggcgttc ttgggcctca
       61 acggataaag tggctgtcgg gcgtgccagc agccgcttca gaccgatagg ttctcccgta
      121 tcggcacaga atccatagtc cccttcatca atattgcgga tggtcgcctg tattttactg
      181 agaagttttc gttcccgatc ccgggtacgg agttccaatg cgtactcttc ttcctgtgtg
      241 gcacggtcgg caggatcggg ggctgattcg tgttcttgga gatgccctgt tgtaacggag
      301 gcgttttcga tgagttcgtc ttgcattttt accagcaatt cgcggaaaaa agccaaatgg
      361 tcgtcattca tataatcgtc ttccggccca atccaattca aaatatcttg ttctgtcagc
      421 tttaccataa tgtatgccct ctcggttcac cgcaaaaaaa caaagcacta caaagtggct
      481 ttattccttt gatttttata aggcttattg cctaaggcgt ttttgaaaac gcgcatcata
      541 acacgttttg gattggtttt ttttattttc tttacattat tgatattgcc gttccatttc
      601 ctgcgaacgg cacacacagg cacaaacgcc ctcacttatg gcttcggcga cacgatgccg
      661 tctgaaggct tccacggctt cgtgggttgt cccgcctttt gacgttacat ttttttgaag
      721 cttctcgaaa tcttcacccg tctgctcggc aagggcaacc gctcctttaa acgttgccag
      781 actgagcgcg cgtgcttctg ccatatcaaa cccttgtcgg atggcggcat tttgcaatgc
      841 gtccagcaga taaaacacat aagccggccc gctgccgctg atgccggtaa tgctgtgcat
      901 ttgcgcttca tcttccaacc aaacggtcaa accgactgat ttcatgattc gatcggcaat
      961 cctgcggtct gtttccgata cttccgcttc ggcatacata ccggatacgc ccagcccgat
     1021 ttttccgggt gtattcggca taacccggac aatgcggcgg cttcccccga ggtaacggct
     1081 gagcgtaccg accgacaatc cggctgcgac agaaagcacc aatgcaccgt tggtgcggat
     1141 atttttgcac gcggcttcca tatcctgcgg tttgacggca aggattaaaa catcgtcgga
     1201 atgaagctcc ggcagggttg ccgaagtttc gacccccaac tcttttccca aacgttcgcg
     1261 cttttccgca ccacggttgg ctatatggat gcggtatccc ccttgtttga ccaatccgcc
     1321 cgcaacggca gccgccatat ttccgccgcc gagaaaataa acattcatta tttctaccct
     1381 attcaaaatt accttactaa attcattccc aattttttac catcggacgc gctcggacag
     1441 gccgttgcgc cgcaacccct gcttttccaa acagtcgaaa ccttcctgtt tgaacctctc
     1501 aaccaaaggt ttgccgtcac gcgtacgcca atccaacccg caggcttcaa acttccctat
     1561 tttcctataa aagtaagatt tttgcgcgga ttcatattcg tttgccctga cggaacgatt
     1621 gccgtctgaa agcggtatct cgtagtcctc taaagacagc ggactctcgc cgccaatatc
     1681 ccaaaaatcg atggcggcag gttttctcgg cttacaccag ccggacaagg acgaacactc
     1741 ataccacccc gccagccggc agcctgcaac cgtaaatgca aacaacgcag ccgttgccgt
     1801 atattttatt acccgaattt ccatcagccc cttttcccga aaatcgcgct gccgatacgg
     1861 acgtgtgtcg caccgcactc aatggcggca ggcatatcgt ccgacatccc catagacagc
     1921 acgtccgcct taacgccagc cgcattgagg tcggcaagca gcttttgcat ggtctgaaac
     1981 tgagttttca actccgtttc actgctgttg gctttggcaa cgcacatcag tccgcgcacg
     2041 acaaggttgg gcagcttcgc cacttccacg gcaagcgcga ccgcttcttc gggcgcgaca
     2101 ccgtgttttg ccgcctcgcc cgcaatgttt acctcgacac acacctgcaa gggcggcatt
     2161 aaggagcgac gctgcctgct cagccggacg gcggtcttca gacggcatac ggtatgcacc
     2221 caatgcgcgc gttcggcgac aaatttggtt ttgttggact gcacatctcc gatgacgtgc
     2281 cacacgatgt cggtcaaatc cgccaattcc tccgttttgc cgtaccactc ctgaatatag
     2341 ttctcgccga aatcacgctg tccggcagcg taaacttcgc ggatgccgtc tgaagggaag
     2401 gtcttgctga cggcaatcag gctgacggaa tgcggctccc tgcccgcctg cagaaccaat
     2461 tttccgatac ggtcggacac ctcacgataa cgttcttgca acaccgtcat agatcatccc
     2521 ctaattaaaa atgattaaac agttgaaacc cctccagtca gggcggtata atcaaggttg
     2581 ttagaatcat tccaaccaat cgaaacatta tactaaacag agccgcatta tgcagattac
     2641 cgacttactc gccttcggcg ctaaaaacaa agcatccgac cttcacctga gttcgggcat
     2701 atcccctatg attcgggttc acggcgacat gcggcgcatc aaccttcccg aaatgagcgc
     2761 ggaagaggtc ggcaatatgg taacttcggt gatgaacgac caccagcgga aaatctacca
     2821 gcaaaacttg gaagtcgact tctcgttcga actgcccaac gtcgcccgat tccgcgtcaa
     2881 cgccttcaac accggccgcg gccccgccgc cgtattccgc accattccca gcaccgtctt
     2941 atcgctggaa gaattgaaag ccccgagcat tttccaaaaa atcgcagaat cgccgcgcgg
     3001 catggtattg gttaccggcc ctaccggttc gggcaaatcg accacgcttg ccgcgatgat
     3061 caactacatc aacgaaaccc agccggcaca catcctgacc atcgaagacc cgatcgaatt
     3121 cgtccaccaa agcaaaaaat ccctgattaa ccaacgcgag ctgcaccagc acaccctcag
     3181 cttcgccaac gcgctgagtt ccgcattgcg cgaagaccca gacgttatcc ttgtcggcga
     3241 gatgcgcgac cccgaaacca tcggcttggc actgaccgcc gccgaaaccg gacacttggt
     3301 tttcggcacg ctgcacacga ccggcgcggc aaaaaccgtc gaccgtatcg tggacgtatt
     3361 ccccgccgga gagaaagaaa tggtgcgttc catgctgtcc gaatcgctga ccgccgtcat
     3421 ctcccaaaac ctgctgaaaa cgcacgacgg caacggccgt gtcgcctcgc acgaaatcct
     3481 gattgccaac cccgccgtcc gcaacctcat ccgcgaaaac aaaatcacgc agattaactc
     3541 cgtcctgcaa accgggcagg cgagcggtat gcagacgatg gaccaatcgc tgcaatcgct
     3601 ggtgcgccaa gggctgatcg caccggaagc cgcacgcaga cgcgcgcaaa acagcgaaag
     3661 tatgagtttc tgacacacaa ccgccttccg gccataccgg cgggaaaaca aggcgcaaac
     3721 acgccgggcg ggacgcggca tcccgcccgg catacccttc cgaacaggaa gcgtccgcct
     3781 tcctgttgaa acctgccgcc gcaaactgca aggcttaaac cgaaaagaag ctaacgatga
     3841 ataccgataa cctgcacgac atcttggacg aaacggttca agtgtattct caaaaaaaac
     3901 aaagccgatc cgaaaccccg gccgaaatcg gcgcacactt ccacccgctg ctcgaccgcc
     3961 tgtgcgaaac cgcagaagca caaaacgcgt ccgacatcct tatcagcaaa ggattcccgc
     4021 cctcgttgaa aatcaacagc gcattaaccc cgcagccgca aaaggctctg acgggcgagg
     4081 aaaccgccgc catcgccgca tcgacgatga acgccgaaca atcggaaata ttccggcgcg
     4141 acggcgaaat caactactcc gtccaatcgc gcagcggcac gcgctaccgc gccaacgcct
     4201 actacagcca aggcagcgca ggtttggttt tgcggcgcat caaccacgtc atcccgcaaa
     4261 tgcgggaatt gggcttgccc gaaaaactca aagacctcgc cgtcgcaccg cgcgggctgc
     4321 tgattatcgt cgggcctacc ggttcgggca aatccaccac gatggcgact atgctcgaac
     4381 accgcaacaa aaccctgccc ggccatatcg ttaccatcga agacccgatt gagtttatct
     4441 acaaaccgcg ccgctgcatc tttacccagc gcgaaatcgg cgtcgacacc ataaactggc
     4501 agacggcggt acaaaacgct atgcgccaat ccccagacgt ggtctgtatc ggcgaagtcc
     4561 gcagcaggga aagtatggaa tacgcgatgc agctcgccca aaccggccac ctgtgcattt
     4621 ttacgctcca cgccaacacc gcgccgcagt cgctcgaacg catactcaac ttctacccca
     4681 aagaacagca caaccaaata ctgattgaca tcgccctcaa tctgaccggc atcatctgcc
     4741 aacgcctcgc cctcaaaaaa gacaaaacgg gcaggacggc ggttgtcgac ttgctcatca
     4801 acacgcccgc catccaagac ttcatcctga agggcgacct gatgaacatc agtaaaatca
     4861 tggaaaccgc caaaaccgac ggaatgcaga cgatggacca aaaccttttc gaactgtacc
     4921 gtcacggcat catcagttac gaagaagccc tgcgccagtc cgtttccgcc aacaacctgc
     4981 gattgcacat ccaactgcac aaagaaggca aaacgcccga actcctttac gacagggtca
     5041 acggtctcaa cctcatttcc tgatccgcaa aacccaatgc cgtctgaaaa ccgcatcccc
     5101 gttttcagac ggcatgattt tatccgcccc attcatgtgc cacactttat tcacttcata
     5161 acaataaacc ggtaaaacca tgaaaacccc actcctcaag cctctgctca ttacctcgct
     5221 tcccgttttc gccagtgtct ttaccgccgc ctccatcgtc tggcagctag gcgaacccaa
     5281 gctcgccatg cccttcgtac tcggcatcat cgccggcggc ctggtcgatt tggacaaccg
     5341 cctgaccgga cggctgaaaa acatcatcgc caccgtcgcc ctgtttaccc tctcctcgct
     5401 cacggcgcaa agcaccctcg gcacagggct gcccttcatc ctcgccatga ccctgatgac
     5461 cttcggcttt accattttag gcgcggtcgg gctgaaatac cgcaccttcg ccttcggcgc
     5521 actcgccgtc gccacctaca ccacgcttac ctacaccccc gaaacctact ggctgaccaa
     5581 ccccttcatg attttatgcg gcaccgtact gtacagcacc gccatcatcc tgttccaaat
     5641 catcctgccc caccgccccg tccaagaaag cgtcgccaac gcctacgaag cactcggcgg
     5701 ctacctcgaa gccaaagccg acttcttcga ccccgatgag gcagcctgga taggcaaccg
     5761 ccacatcgac ctcgccatga gcaacaccgg cgtcatcacc gccttcaacc aatgccgttc
     5821 cgccctgttt taccgtttgc gcggcaaaca ccgccacccg cgcaccgcca aaatgctgcg
     5881 ctactacttc gccgcccaag acatccacga acgcatcagc tccgcccacg tcgactacca
     5941 agagatgtcc gaaaaattca aaaacaccga catcatcttc cgcatccacc gcctgctcga
     6001 aatgcaggga caagcctgcc gcaacaccgc acaagccctg cgcgcaagca aagactacgt
     6061 ttacagcaaa cgcctcggac gcgccatcga aggctgccgc caatcgctgc gcctcctttc
     6121 agacggcaac gacagtcccg acatccgcca cctgagccgc cttctcgaca acctcggcag
     6181 cgtcgaccag cagttccgcc aactccgaca cagcgactcc cccgccgaaa acgaccgcat
     6241 gggcgacacc cgcatcgccg ccctcgaaac cggcagcttc aaaaacacct ggcaggcaat
     6301 ccgtccgcag ctgaacctcg aatcaggcgt attccgccat gccgtccgcc tgtccctcgt
     6361 cgttgccgcc gcctgcacca tcgtcgaagc cctcaacctc aacctcggct actggatact
     6421 gctgaccgcc cttttcgtct gccaacccaa ctacaccgcc accaaaagcc gcgtgtacca
     6481 acgcatcgcc ggcaccgtac tcggcgtaat cgtcggctcg ctcgtcccct acttcacccc
     6541 ctccgtcgaa accaaactct ggatcgtcat cgccggtacc accctctttt tcatgacccg
     6601 cacctacaaa tacagttttt ccaccttctt catcaccatt caggcactga ccagcctctc
     6661 cctcgcaggt ttggacgtat acgccgccat gcccgtgcgc atcatcgaca ccattatcgg
     6721 cgcatccctt gcctgggcgg cggtcagcta cctgtggcca gactggaaat acctcacgct
     6781 cgaacgcacc gccgcccttg ccgtatgcag cagcggcaca tacctccaaa aaattgccga
     6841 acgcctcaaa accggcgaaa ccggcgacga catagaatac cgcatcaccc gccgccgcgc
     6901 ccacgaacac accgccgccc tcagcagcac cctttccgac atgagcagcg aacccgcaaa
     6961 attcgccgac agcctgcaac ccggctttac cctgctcaaa accggctacg ccctgaccgg
     7021 ctacatctcc gccctcggcg cataccgcag cgaaatgcac gaagaatgca gccccgactt
     7081 taccgcacag ttccaccttg ccgccgaaca caccgcccac atcttccaac acctgcccga
     7141 catgggaccc gacgactttc agacggcatt ggatacactg cgcggcgaac tcggcaccct
     7201 ccgcacccgc agcagcggaa cacaaagcca catcctcctc caacagctcc aactcatcgc
     7261 ccggcagctc gaaccctact accgcgccta ccgacaaatt ccgcacaggc agccccaaaa
     7321 cgcagcctga aaaaatttcg gcattttgta aaggggggcg gattgtcagg caggttgcaa
     7381 gatagtggat aaggctttgc gcagggtaaa tgcgtagcaa ctgaaccgtc attccctcaa
     7441 aaacagaaaa ccaaaaacag aaacctaaaa tcccgtcatt cccacgaaag tgggaatccg
     7501 gttttttgag tttcagtcat tcccgataaa ttgccttagc attgaatgtc tagattcccg
     7561 cctgcgcggg
//
LOCUS       DAXDSD010000095         7479 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXDSD010000095 DAXDSD010000000
VERSION     DAXDSD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.095"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            145..294
                     /locus_tag="ACFB2Q_001743"
     CDS             145..294
                     /locus_tag="ACFB2Q_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918719.1"
                     /translation="MNAEGFCEKQTACRPDGRRNAGDRADLKAFEFAGRELANYREML
                     KRYAV"
     gene            324..3371
                     /locus_tag="ACFB2Q_001744"
     CDS             324..3371
                     /locus_tag="ACFB2Q_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFC0918720.1"
                     /translation="MSDLEAKVRITVENHTKQGFDSAAADADKAAEKMRSSGDNAAKG
                     FKAAIDSMHETMRNLHADVKAGFEAAGNQAQQASEKVRAEVGKIGSGLSGLTKLLAGL
                     ATADFAKSVLDTADAMQSINSQVRQVTSSETEYLAVQQQLLDTANRTCASLESTANLY
                     VSTSRALKDYGYTQQEILKFTEATNNAMTIGGVGARQQAAALFQLSQALGSGVLQGDE
                     FKSISESAPILLDTIAEYMGKSRDEIKKLGSEGKLTADVIFKAISGASEKFGEQAAKM
                     PVTMGQALTVFSNNWQSMVSKLLNDSGTMSGIAAVIKLIADNLNLVVPIVAGFAVAVA
                     AAVAPTLALNLALLANPFGIIAVAIGTVVGLIAKFGDEIDVFGGGWSNLSDVIRAVWQ
                     IITETVGEAVGTVKSWFDGLTGRLNEGAGGWPSLFGRVMSVISSAIGAYVNVYINIFA
                     TGWMLIKEAANDMPQFFANLGKAIGNVFISAIERMINKAVGMINSMIDFANKAASMAG
                     ISGIGKLNKVRMGRMDDGGLGERIHDSLTKDRAGAMANAVRERAADIHEADALKGRGG
                     GGHAKTARKKPGANQGGGKGGKSRSGGPGAAKDPMQAWEGEIKARKLAHREMQRETLT
                     HQEWDLAREAEYWRAKLATVDANGKTGVKIREKILTLEDQLSKQSTEAKMNQAAEWEK
                     LDKHKLEMEKDAADQALADGRISQLERLDLEIEFENRRYRIAYDALQERIALAEQDPA
                     YSQAAIGKLKAQMGELGRGHERTQAKNEGKRENQRRKDAPNVMEMLQDGGRNVWQEAQ
                     QQMGQAFTAMLARAQNFRQAMNGFFKSMGQTFIQEMVTKPLTGLMRRMVEESAIYKMI
                     FGTKETLETAAAAKTAATKATETTAVVSSNAVQAASGAAASQAHIPYVGPILAVAAMA
                     AMMSAVMGLMGGGGGSQTTTTTTRIPSAAGGWDIPAGINPLTQLHENEMVLPAEHAQT
                     IREMADQQGGGSTVIINSTGGDFIHKNDLAKLLKQMKRDFKFV"
     gene            complement(3431..3910)
                     /locus_tag="ACFB2Q_001745"
     CDS             complement(3431..3910)
                     /locus_tag="ACFB2Q_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFC0918721.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(4252..5241)
                     /locus_tag="ACFB2Q_001746"
     CDS             complement(4252..5241)
                     /locus_tag="ACFB2Q_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFC0918722.1"
                     /translation="MATITQRNGKWRVQIRMKGVSRSATFERASDAKAWAARIESQIM
                     DGIQGNAPRNTIFADLIRRYLSEVTPSKRGAREESYRIGRALKTPLAKVRLADLRPQD
                     FADWRDQRLQEVSPTSVGRELTTLSAVCEHAMKEWGLLRENPVRKISKPKKSRARTRR
                     PTEQEIADICAALLYRPNEKPKMAVQRVAVAVLFAIETAMRAGEICGLKWADVNMRRR
                     IAHLPITKNGDSRDVPLSLRAAELIEQLRGIDDTWVFSLDAKSLDVLFRRARDNCGIQ
                     GLHFHDTRREALTRLSKKVPVEVLAKISGHRDLRILLNVYYRPDMADIAKMLD"
     gene            complement(5501..5689)
                     /locus_tag="ACFB2Q_001747"
     CDS             complement(5501..5689)
                     /locus_tag="ACFB2Q_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918723.1"
                     /translation="MADGRKGFFDAIIKINIFFFPVKTPTLGAANPIAGGSTRFRFGA
                     EQMAVLYVPFCVLKHIGR"
     gene            5930..6964
                     /gene="purM"
                     /locus_tag="ACFB2Q_001748"
     CDS             5930..6964
                     /gene="purM"
                     /locus_tag="ACFB2Q_001748"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC0918724.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGLSVGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
BASE COUNT         1886 a         1826 c         2053 g         1714 t
ORIGIN      
        1 atccggacaa caaccgatgc cgcaccctgc gggcaggctt cgcactctga aaggctttcg
       61 gacggcattg ttcttacctg cgcgtcaaac cgccttcgtg taaagcggtt gcaaaaaagc
      121 cttttatttt gacagggtgc agagatgaac gccgaaggat tttgtgaaaa acaaaccgcc
      181 tgccggccgg acgggcgccg aaatgcgggc gatcgtgccg atttgaaggc ctttgagttt
      241 gccggacggg aattggcgaa ttatcgggaa atgttgaaac gttatgccgt ctgaaaagac
      301 ggctttttta tttggggctg taaatgagcg atttggaagc caaagtcagg ataacggtag
      361 aaaaccatac gaagcagggt tttgattcgg ccgccgccga tgctgataag gcggcggaaa
      421 agatgcgcag cagcggcgat aatgccgcaa agggctttaa agctgcaatc gacagtatgc
      481 acgagaccat gcgcaatttg cacgccgatg tgaaagcggg atttgaagcg gcgggcaatc
      541 aggcgcaaca ggcgtctgaa aaggttaggg ccgaagtggg taaaatcggc tcaggcttat
      601 cggggctgac caaactgctg gcaggattag caaccgcaga cttcgccaaa tcggtgcttg
      661 ataccgccga tgcgatgcag tcgataaaca gccaagtccg acaagtcacg tcgtctgaaa
      721 cggagtatct ggccgtgcag cagcagcttc tcgatacggc aaaccgtacc tgtgcatcgc
      781 ttgaatcaac ggcaaacctg tacgtttcca caagccgcgc gttgaaagac tacggctaca
      841 cgcagcagga aattttaaaa ttcaccgagg caaccaacaa cgcgatgacc atcggcggcg
      901 ttggcgcgcg acaacaggcc gccgcgctgt tccagttatc gcaggctttg ggcagcggcg
      961 tattgcaggg cgatgaattt aaatccattt ccgaatccgc acctattttg ctggatacca
     1021 ttgcggaata tatgggcaaa tcccgcgacg aaatcaaaaa gctgggcagc gaagggaaat
     1081 tgacggcgga tgtgattttc aaagccattt ccggcgcgtc ggagaagttc ggggagcagg
     1141 cggccaaaat gcccgtgacg atggggcagg ctttgacggt gttctcgaac aactggcaaa
     1201 gcatggtttc aaaactgctg aacgacagcg gcacaatgtc ggggattgcc gccgttatta
     1261 aactgattgc ggataacctt aatttggtcg tcccgatcgt cgcaggtttt gccgttgccg
     1321 ttgccgccgc cgttgcaccg acactggctt tgaatcttgc actgctggca aacccgttcg
     1381 ggattattgc cgtcgcaatc ggcacggttg tcgggctgat tgcgaaattc ggcgatgaaa
     1441 tcgatgtttt cggcggcggc tggtcgaatc tttccgatgt gattcgggcg gtttggcaaa
     1501 tcatcacgga aaccgtcggg gaagcggtgg gaaccgtcaa atcgtggttt gacgggctga
     1561 caggccggct gaacgagggc gcgggcggct ggccgtcgtt gttcgggcgc gtgatgagcg
     1621 tcatctcaag cgcaatcggc gcgtatgtga acgtttatat caacatattc gcaaccggct
     1681 ggatgctgat taaagaagcc gcaaacgata tgccgcaatt cttcgccaat ctcggcaagg
     1741 ccataggcaa cgtgtttatt tccgcgatcg aacggatgat aaacaaagcg gtaggcatga
     1801 ttaacagcat gattgacttt gccaacaaag ccgcgtcgat ggccgggata tcgggcattg
     1861 gaaagctgaa caaggttcgg atggggcgga tggacgacgg cgggcttggc gagcgtattc
     1921 atgacagctt gacgaaagac cgcgccggcg caatggcaaa tgccgtccgc gaacgggccg
     1981 ccgatattca cgaagccgat gccctcaagg gacgcggcgg cggaggacac gccaaaaccg
     2041 cccggaaaaa gccgggcgca aatcagggcg gcggcaaagg gggcaaatcc cgttcgggcg
     2101 gtccgggcgc ggccaaagac ccgatgcagg cttgggaagg ggaaatcaaa gcccgaaaac
     2161 tcgcacaccg cgaaatgcag cgcgaaacgc tcacgcacca agaatgggat ttggcgcgtg
     2221 aggcggaata ttggcgggcg aagctggcaa cggtggacgc caacggcaaa acgggcgtaa
     2281 aaatccgcga aaaaatcctg acgcttgaag accaattatc gaagcagtca accgaagcca
     2341 aaatgaatca ggcggccgaa tgggagaaat tggacaagca caagctggag atggagaaag
     2401 acgcggcaga ccaagccctg gcagacggac gtatttcgca actcgaacgc ctggacttgg
     2461 aaatcgagtt tgaaaaccga cgttaccgga ttgcctatga cgcattgcag gaacgcatcg
     2521 cacttgccga acaagacccg gcatacagtc aggcggcaat cggcaagctc aaggcccaaa
     2581 tgggcgaatt ggggcggggg cacgaacgga cgcaggcgaa gaacgagggc aaacgcgaaa
     2641 accaacgccg caaagacgcg cccaacgtca tggaaatgct gcaagacggc ggcaggaacg
     2701 tttggcagga agcacagcag cagatggggc aggcgttcac cgccatgctg gcgagggcgc
     2761 agaacttcag gcaggcgatg aacggctttt tcaagagtat ggggcaaacc tttattcagg
     2821 aaatggttac gaaacctttg acgggcttga tgcggcgcat ggttgaggaa tcggccattt
     2881 acaagatgat tttcggaact aaggaaacgc tggagacagc ggcggcagcc aagacggcgg
     2941 caactaaggc aaccgagaca acggcggttg tcagtagtaa tgctgttcaa gcggcttcag
     3001 gcgcggcggc ttcccaggcg catatcccat atgtcggccc gattcttgcc gttgcggcaa
     3061 tggcggcgat gatgtcggct gtaatgggac tgatgggcgg cggcggcggt tctcaaacga
     3121 ccacaaccac gacgcgcatt ccctcggcgg caggcggctg ggacatcccg gcaggcatca
     3181 acccgctgac ccaactgcac gagaacgaga tggtcttacc cgcagaacac gcccaaacca
     3241 tccgtgaaat ggcggatcag cagggaggcg gcagcaccgt catcatcaat tcgacaggag
     3301 gcgacttcat ccataaaaac gatttggcga agctgttgaa acagatgaaa cgggacttta
     3361 aatttgtctg agaaaatgcc gtccgaaagg ttcggacggc ctatgttgcg gcgtacgtca
     3421 agctattaag ctatttggtt ttcagaggct ttttcttcca tttccgggct acggcttcaa
     3481 attcttgaaa ggccgtcggc gcgctcctga tacggcgtat ttcaaaaatg aagctgctta
     3541 ggttgtccca gtcgcgtaac agcatggagt attcgaaatc tttatacatt ttctcgtgca
     3601 gcgccccgcc caatacgccc gcacagacaa attcgcgctg gtttaaaact gtcagtatgg
     3661 cttcacggtc tttcttcttg tccggggtat ctgatgtata ggtggcgagt atgcatccgt
     3721 ctgtttttgc cagcccgttt actatggtta tggcttcttg aagggcggca ttgttacgtt
     3781 cggccatgat catgtcgatg gtggcgcgct ttttggcgtt ttccgtattc ctcatgatgc
     3841 cgtaagcggc aacaaaaacg ccgataacag tcaatatcgg cgttgctatc tgtattaggt
     3901 tgtcagtcat tgccgtccca gccgtccgaa aggcggaaat cacggctttc cgaataaata
     3961 tgccccattg tcatcccctt aagctgatgt tgagaataaa tccttgcgtc ggaatatacg
     4021 gcattgcctg actattttca agattccgca ccccgcggac cggactatac aaaaaaaccg
     4081 ccttcgtgta aagcggttga agagagcctt tcaataaaaa tgccgtccga agggctttcg
     4141 gacggcattt tttatagatt cttttttaat gaacctttga aaccgaagat aattaatttt
     4201 cctctttatt ttcaatacgc agtgtgccct atgtttttaa ataaaaatat tttaatccaa
     4261 catttttgca atatccgcca tgtcggggcg gtagtaaaca ttgagtaaaa tccttaaatc
     4321 ccgatgcccg ctgatttttg ccaagacctc cacaggcact ttttttgata ggcgcgtgag
     4381 ggcttcgcgg cgggtgtcgt gaaaatgcag gccttgtatg ccgcaattat cccgcgctcg
     4441 cctgaataat acatcaaggc ttttggcatc caggctaaac acccatgtgt catcaattcc
     4501 gcgcagttgc tctattagtt cggcggctcg caaggataat ggcacgtcac ggctgtcgcc
     4561 gtttttagtt attgggaggt gggcaatacg acgccgcata ttcacatctg cccattttag
     4621 gccgcagatt tcgcctgccc gcatggcggt ttcgatggca aataggacgg caacggcaac
     4681 ccgttgcacc gccattttcg gtttctcatt aggccggtat aggagggcgg cgcaaatatc
     4741 ggcaatttcc tgctcggttg gccgtcttgt ccttgcccgg ctttttttcg gcttgctgat
     4801 tttgcgtaca gggttttcgc gaagaagtcc ccactctttc atggcgtgtt cgcagacggc
     4861 ggataaagtg gttaattcgc gtccgacgct ggtgggggat acctcctgca accgttgatc
     4921 tcgccaatcg gcgaagtctt gagggcgcag gtcggcaagc cgcacctttg ccaaaggtgt
     4981 ttttagtgcg cgtccaatgc ggtatgattc ttcccgtgcg ccccgctttg atggtgtaac
     5041 ctcggataaa taccgccgga taaggtcggc aaagatagta tttcgcgggg cattgccttg
     5101 gatgccgtcc ataatttgcg actctattcg cgccgcccac gccttcgcgt ctgatgcccg
     5161 ctcaaatgtg gcggaacggg aaacgccttt catacggatt tgtacgcgcc atttgccgtt
     5221 acgttgagtg atggttgcca tatttattcg ggtgcaattc gggtgcaaat ataatagagc
     5281 aaaacagata taaagcgggg caaaataggg ctatattaaa tttaatgctt tgatatgtag
     5341 ggataaatag atttaaaaag acgaaagaag tgcggacgta gaataagcgt agatgcgctc
     5401 cgtccgcacc aataaaaata tataaatcat attgttaatg attattggga caattcggtg
     5461 gcacaaaata taagctgtct gcttttcagc gggcggcttt ttatctgcct atgtgtttca
     5521 acacacaaaa cggcacataa agcaccgcca tttgttccgc accgaatcgg aagcgggtgc
     5581 ttccgcccgc aatcggattc gccgccccta atgtcggggt tttaaccgga aagaagaaaa
     5641 tattgatttt gatgatggca tcaaaaaaac ccttccgccc gtctgccaaa aggaatggtt
     5701 aagaaaagca atgcgtcttt tgatggaacg cgatatataa gaaattgagg ggaagggcag
     5761 gcgggtttct gcccgttaat cctgcggacg gccccgccaa acccgacacg cgcgggcata
     5821 tggcggattg tttacaatat tgtctaaaaa tctgaacctg caaacgcgaa ataaggtaga
     5881 atacgccccg ttattttacc gtccaatccc catttaccaa ggaaaaacga tgagtacttc
     5941 attgagttac cgcgatgcgg gcgtcggtat cgacgcaggc gaccaactgg tcgaaaaaat
     6001 caaaccgttt gccaaacgca ccatgcgtcc ggaagtattg ggggatttgg gcggttttgg
     6061 cgcattggtc gaaatcggca aaaaatatca aaaccccgta ttggtcagcg gtacggacgg
     6121 cgtgggtacc aagctcaaac ttgccttcga ttgggacaaa cacgatacgg tgggcatcga
     6181 ccttgttgca atgagtgtca acgatatttt ggttcaagga gctgaaccat tgtttttctt
     6241 ggattatttt gcctgcggta aattggatgt tccgcgtgcg accgatgtca tcaaaggcat
     6301 tgcccaaggt tgcgaagaat ccggctgcgc cctgattggc ggggaaactg ccgaaatgcc
     6361 gggtatgtat cccgttggtg aatacgattt ggcgggtttt gccgtcggcg tggtggaaaa
     6421 agagaatgtc attaccggcc tcagcgtcgg cgcgggcgat gtggtattgg gtttggcttc
     6481 caacggcgca cattcaaacg gctattccct tatccgtaaa atcatcgaac gcgacaatcc
     6541 cgatctggat gccgagtttg ataatggcaa aactttacgt gaagccgtta ttgcgccgac
     6601 acgtctgtat gtgaaaccga ttcttgccgc tttggaaaaa tttaccatta aaggtatggc
     6661 gcacattacc ggcggcggca ttaccgaaaa cgtgccgcgc gtgttgccta aaaacacggt
     6721 tgcccaaatc gatgccgaat cgtgggaatt gcccaagctc ttccaatggc ttcaaaaggc
     6781 gggcaatgtg gaaacccaag aaatgtaccg gacctttaac tgcggcatcg gtatggtcgt
     6841 catcgttgct gccgaagatg ccgatgcggt tcggtcattc ctgagcgggc agggcgagac
     6901 agtgtaccgt ttgggttgca tccgagagcg tcaagggaac gagcatcaaa cccaagttgc
     6961 ctgacttcca agcatcaaat gccgtctgaa tttgtttcag acggcatttc cgcatcttcc
     7021 aaacgcgtat ccgcaacaca gttctgcaaa ctgatatttt gcccggtatg gcggcttaaa
     7081 cttcttcatc atatttcgat aatgctataa aatgatataa tcataaaata ttgtttattc
     7141 ctacatcgta ttcatcctat tttccgaaat gtgagcgttt tatgaagcaa tccgtccata
     7201 ataaaacaat tttaagtgtt ttgatttcat ctatgtctgt aacggctttt tccgcccccc
     7261 accccccccc gcgataaatg ctatttcagt caatcgtacc gattccggcg aactcatcgg
     7321 ttttgagttt aataatgcgg acaataccgt aaccggcaat gcggatatta cggtcaaacc
     7381 tgccgaccct tcacagcatt cgcgcccgaa agggctgatt atccggagcg accgtaacga
     7441 accaaacaaa aaccaagaag ttcgcttaaa taatatagc
//
LOCUS       DAXDSD010000096         7458 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXDSD010000096 DAXDSD010000000
VERSION     DAXDSD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.096"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(191..1198)
                     /locus_tag="ACFB2Q_001749"
     CDS             complement(191..1198)
                     /locus_tag="ACFB2Q_001749"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC0918725.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            1430..1666
                     /gene="acpP"
                     /locus_tag="ACFB2Q_001750"
     CDS             1430..1666
                     /gene="acpP"
                     /locus_tag="ACFB2Q_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC0918726.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1822..3069
                     /gene="fabF"
                     /locus_tag="ACFB2Q_001751"
     CDS             1822..3069
                     /gene="fabF"
                     /locus_tag="ACFB2Q_001751"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC0918727.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            3462..4687
                     /locus_tag="ACFB2Q_001752"
                     /pseudo
     CDS             3462..4687
                     /locus_tag="ACFB2Q_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4692..4814
                     /locus_tag="ACFB2Q_001753"
     CDS             4692..4814
                     /locus_tag="ACFB2Q_001753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918728.1"
                     /translation="MPSETFRRHFPNTPPVAGLHGTQISLIKPQITAAIKPPFK"
     gene            5041..6168
                     /locus_tag="ACFB2Q_001754"
     CDS             5041..6168
                     /locus_tag="ACFB2Q_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC0918729.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGVNPFSDIKSTFLIFKALKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRITLPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKGKYPDTVFTALGAIDKPRGSGDLEQPAARDIIRFPGFVNNVSEVIKEHH
                     IFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENRAAVRLMGNASYAIAKDKFDAEKVDLKFLDILKA"
     gene            complement(6570..7420)
                     /locus_tag="ACFB2Q_001755"
                     /pseudo
     CDS             complement(6570..7420)
                     /locus_tag="ACFB2Q_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
BASE COUNT         1696 a         2106 c         1951 g         1705 t
ORIGIN      
        1 ctccggttgc ctgccccgtg ttcacgcatc ggtttgcctg ccccgtcatt ccgccccttt
       61 cgattgacgc ctgttacgaa aagtcttcca aatgacggct gattgcctct tcaacagtcg
      121 gataccaatc tatgaaaatg ccgtctgaac ggcaaaacgt gcgttcagac ggcattttgg
      181 gtggggcgcg tcatcgcgcc aatgccttca aacattcttt gaccaacgcc gggcctttgt
      241 atatcaatcc gctgtacact tggacggcgg tcgtgcccaa gcggattttc tctgccgcgt
      301 ccccgccttc cataatgccg cctacgccga taatcggcaa tgcgccgtct atgtgttccg
      361 ccagtttttt cagcacccgg ttgctttttt cgcgcacggg aaaaccgctc aaaccgccct
      421 gctcgcctgc gagcggatgg ctgccgaggc ttgatttgtc gatggtggtg ttggtagcga
      481 tgatgccgtc catttcgacg gatttgacga cgcgggcgat gtcttcgatt tgtgcttcat
      541 ccaaatcggg ggcgattttg acggctagcg ggatgtattt gccgtgggcg gcggcaagct
      601 gcgcctgttt gtttttcagg gcttcgagca atgcgcccaa ttcgccgccg ccttgcagcg
      661 cgcggaggtt tttggtgttg ggcgaggaaa tgttgacggt aatgtagctt gcgtgcgcgt
      721 aggctttttc gaggcagatt aaataatcat cggcggcgtt ttggatgggc gtaaccgcgt
      781 ttttgccgat gttgatgccc aatacgcctt gatatttgct tttttcaata ttgcgtatca
      841 tcgcgtcgat gccgtggttg ttaaaaccca ttcggttgat gatgccttgg tgttcgggaa
      901 cgcggaagag gcgcggctgc gggttgccgg gctgcgggtt gcgcgtaacc gtgccgattt
      961 cgaggaagcc gaaaccgagc gcgcccaaag cgtcgatgta ttcaccgttt ttgtcgagac
     1021 ctgcggcaag cccgacagga ttgggcaaat ccatacccat cagttttata ggtttggtac
     1081 ggttgtcggt tacgggaatc aggcccaatt tataaaccgt gttgagcgcg tcgagcgtga
     1141 agtggtgggc tttttcggca tcgagtgcaa acaggatgcg gcgggcaagt ggatacatga
     1201 cagggctttc tgcttcagat ggaaatgccg ccattttaac cgaaatcccg ccaaacatac
     1261 ggcacacagg tcaaacttga acgacaaaac ccgccgcacc caaaaatgta caaatccgca
     1321 aatattgata taataggcat tagatagaaa actctattaa aaatcggtac aataccaccg
     1381 atttatttcc cttaaacaac ctgccgcaag gcataaagga acgactgata tgtcaaacat
     1441 cgaacaacaa gttaagaaaa ttattgctga acaactgggc gtaaacgaag ccgacgtgaa
     1501 aaacgaatct tccttccaag acgacttggg cgcggattct ttggataccg tggagctggt
     1561 tatggctttg gaagaagcct tcggctgcga aatccccgac gaagatgccg aaaaaatcac
     1621 caccgtccaa ctggctatcg actacatcaa tgcccacaac ggctaaccgg tcgtcgcccg
     1681 acacaacagc ctctgctgca ccgcgcaata gaggcttttc ccttatatgg caaactgttt
     1741 gaacccctgc cgcaaattac agacggcatc tgcacgacag ccgcatatgc ccccattccg
     1801 acaaccaaca gcgaggttat catgagtcag agaagagtag tcattacagg cttaggtcag
     1861 gtttcccctg tcggcaacac tgccgcagag gcttgggaca acctgctcgc cggcaaaagc
     1921 ggcatcggcg cgattacccg ctttgacgca tccgacatca acagccgtgt cgccggcgaa
     1981 gtgcgcggtt tcgacatcgg acaatacatc agcgcgaaag aagcgcgccg gatggacgtg
     2041 ttcatccact acggcatcgc cgccgcattg caggcgattg ccgattcggg tttggacgat
     2101 gtggaaaacc tcgacaaaga ccgcatcggc gtgaacatcg gttccggcat cggcggactg
     2161 cccagcatcg aggccaccgg caaagccgta atcgaaggcg gcgcgcgcaa aatcaaccct
     2221 ttctttatcc ccggttcgct gatcaacctg atttccggac acgttaccat cctcaaaggc
     2281 taccgcggcc cgagctacgg catggtttcc gcctgtacca ccggcgcgca cgccatcggc
     2341 gattccgccc gactgatcaa atacggcgac gcggacataa tggttgccgg cggcgcggaa
     2401 ggcgcaatca gcaccttggg cgtgggcggt tttgctgcga tgaaagccct ctccacccgc
     2461 aacgacgacc ccgccaccgc ttcccgtccg tgggacaaag gccgcgacgg cttcgtcatc
     2521 ggcgaaggcg cgggcatatt ggtgttggaa gaattagaac acgccaaaaa acgcggcgcg
     2581 aaaatctacg ccgaaatcgt cggcttcggc atgagttccg atgcttacca tatcaccgcg
     2641 ccgaacgaag aaggccccgc ccttgccgtt acccgcgcgc tgaaagatgc cggcatcaat
     2701 cccgaagacg tggattacgt caacgcgcac ggcacgtcca cccccttggg cgatgccaac
     2761 gaaaccaaag ccctcaaacg cgcgttcggc gaacacgcct gcaaaaccgt catcagctcg
     2821 accaaatcca tgaccggcca cctgctcggc gcggcgggcg gcgtggaggc cgtgtacagc
     2881 attttggcga tacacgacgg caaaatcccg ccgaccatca acatttttga acaagacgtt
     2941 gaagccggct gcgatttgga ctactgcgcc aacgaagcgc gcgacgcgga aatcgacgtt
     3001 gccatttcca actccttcgg cttcggcggc accaacggta cgctggtctt caaacgcttc
     3061 aaaggctgat tccgcaaagc cgccgccgac atcgaaatgc cgtctgaaac cgtttcagac
     3121 ggcattttta tagtggatta acaaaaatca ggacaaggcg gcgggccgca gacggtacaa
     3181 atggtacgga accggttcgc ccggtgcttc agcaccttag ggaatcgttc tctttgagct
     3241 aaggcggggc aacgccgtac cggtttaaag ttaatccact atactaactg gaattaacaa
     3301 atacggcaca aatattaacc cgaaccgcaa acgcccatca agccctttca gacggcatcg
     3361 gcagacatac cgttggctat atccaaagaa tcaggaatta tttagcccga atcatccggt
     3421 ttgttaaaat tcgcaaactt aaccgactca aaatacatat tatgacgcac accgcatcga
     3481 aaacacccaa actctgggcg gtcattgccg ccgccgcatt catcctgctg attaccatcg
     3541 gcatgaggat gacgctcgga ctgttcgtcc aacccgtcgt caacaccacc gaattgaaca
     3601 ttgcccagtt cagcctcatc atcgccgttt tccaactgat gtggggcgta ttgcaaccat
     3661 tgtccggcgc gcttgccgac cgtttcggcg cgttcagggt attaagcggc ggtgccctcc
     3721 tgctcgtctg cgcctgcctg attgcctcca acatcccgac ttattggggg ctgatgattg
     3781 ccgtcgggct gctgctcgca ttcggcaccg gttccggcgg gttttccatc attatgggac
     3841 aggtcgccgc ccaagtcccc acacacaaac gcggcttggc atccggactg gtcaacgcag
     3901 gcggttcggc aggacaattc ctgttcgcac cgctggttca aggactcaag gactcgtcgt
     3961 cctgcccgaa gtcggctgga cgggtacatt ttacgtttgg ggcgcaatcg ccctgctgat
     4021 tctgcccgtc tcatggtggc ttgccggcgg caacaacgcc gcccacaccc aacacaccca
     4081 agccacacac ggacaaagcc tcggagaagc agtcaaaacc gccttcaaaa ccccaagcta
     4141 catcctgctg cacctgagct ttttcgcctg cggcttccac atcgcctttc tcgtaaccca
     4201 cctacccacg gaagtcgccc tgtgcggact gcccgccacc gtcgcctcga catccatcgc
     4261 catcatcgga ctggcaaaca tcgccggttg cgtgttctca ggctgatgca ccggacgctt
     4321 caaaggaaaa cacatcctgt tcggactcta cgcctcacgc gtcgccatgg tgctgattta
     4381 catcttctca cccaaaaccg accccaactt ttacattttc gccgccgcac tcggattcac
     4441 atggctcgcc accgtcgccc cgaccgccgc cgttaccggc aaactcttcg gcacgcgcta
     4501 cctcgccacc ctgttcggac tggtgatgct cacccaccaa atcggcggat tcctcggctc
     4561 gtacatcggc ggcatcgtga ttacacaatt cggtgactac ggctggatgt ggtacgccga
     4621 tgccgtactc gccggcacgg cagccctgct tgtcctgccc gtccgcgaac cccgaaccgc
     4681 cgcatgacag aatgccgtct gaaaccttca gacggcattt tccaaacacc ccgcccgttg
     4741 ccggactgca cggcacgcaa atcagcctta tcaaaccgca aataaccgcc gcgattaaac
     4801 cgccattcaa ataagcccgt tttcccttcg ggtgtaaaat catcacaaaa cgcgatactg
     4861 tatatcgctg ggcgttatgc agcggatttc aggataaaat gccgtctgaa gccgcccctg
     4921 cccgggcggg cgaagcgtcg gatgcggcgc gcttggcaaa ggtttgcccg ccgcgccccg
     4981 tatccggcat ttcatcaatg cagaccgcat ttccaatcct atattcaagg tgtccgcctt
     5041 atgaaaatcg tttttatcac aacagtcgca tccagcattt acggtttccg cgcccccgtc
     5101 attaaaaaat taatcggcaa aaaccatcag gtgtatgcct ttgtatcgga gttttccgac
     5161 aatgagttgg acattatcag ggaaatgggg gttacacccg ttacctaccg gtcaaaccgc
     5221 agcggggtaa acccgttttc cgatataaaa tccaccttcc tcatatttaa agcactcaaa
     5281 aaaatatcgc cggatttggt tttcccttat ttcgcaaaac ccgtgatttt cggcactttt
     5341 gccgcaaaat tggcaggcgt gcccagaatc gtcgggatgc tggaaggttt gggattcgca
     5401 tttaccccgc agccggaagg cataccgtta aaaacaaaaa tcataaaggg gattttaatc
     5461 gccttgtacc gcattaccct gccgatgttg gaaagcctga ttgtattaaa ccccgacgac
     5521 aaagacgagt tgctgcatca atacggcatc aaaataaaaa acatccatat tttgggcgga
     5581 atcggtctgg atttgcggca atatccttat tccgaggcgg atattcccga tgaaaaagaa
     5641 cccgtaaaat tcctctttat cggcagattt ctgaaagaaa aggggattga tgattttatt
     5701 cgggcggcgg aacaggttaa gggcaaatac cccgatacgg tttttaccgc tttgggcgca
     5761 atcgacaaac cacgcgggag cggggatttg gaacagcctg ccgcccgcga tattatccgt
     5821 ttccccggtt ttgtaaacaa tgtttccgaa gtgataaaag aacatcatat attcgtattg
     5881 ccgtcttatt atagggaagg cgttccccga agcacccagg aggcaatggc cgtcggcagg
     5941 gcggtgatta cgacggatgt ccccggatgc agggaaacgg ttgccgacaa ggtcaacggc
     6001 ttcctgatcg aaccttggaa tccccgcatc ttggccgaaa aaatgattta ttttatcgaa
     6061 aacagggctg ccgtccgcct gatggggaat gcaagttatg cgattgccaa agataaattc
     6121 gatgccgaaa aagtcgattt gaaatttctc gatattttga aggcgtaaac aaggtggtcg
     6181 gcttttgagt ttcggtgatt tctgacaaac tcccgccatt cccgcgaaac cccgtcattc
     6241 ccgcgaaagc gggaatccag gttcgttgag tttcagatgt gtagggcggg ctgtagggtg
     6301 ggcttcagcc caccgaaacc aatcccgcca ccaacctcac cggctcaaac cggcacagca
     6361 acttatatcc cgtcattctc gcgcaggcgg gaatccagcc ccgccggtac agaaacttat
     6421 cgaaaaaaag tttcttcaga ttttacgttc tagattccca ctttcgcggg aatgacggga
     6481 tgcaggtttc cgcgcggacg gattcggcat tcccgctttc gcagaaatgt cggcttttgg
     6541 tttgttgtct ttataagatt attcggtaat tattctgcgg ttttgcgttt gtgtgctgcc
     6601 ggaatctcaa gggattctct gtatttggca acggtgcggc gggaaacctc tataccgcgg
     6661 aaagccagca ggttggcgag tgcctcgtca gaataggact tgggcgtgga cgaggcaaaa
     6721 ccggcggcgg gaaaggggtt gagcgaagca atgaggccga gtgcggcttc gagtgtgccg
     6781 ctgtcggttt ggggcaggcg ttttttattc gcgccggggt ttggctgcgg ttgccgtcaa
     6841 tgctgtcgag ggcgtttcgg acgatatgca gggcggcggg tttggcagca cattcgcccg
     6901 atctttctat ctgcaatatc aacgattcgg tcacatcggc ggcggccatg cctgccggat
     6961 cgaatttttt caatgcggtc agggcttgtt tcagcattgc ttcatccaac atccactcca
     7021 agggcgtatg gtccaggatg tcttcgatgc tgtcggtcag gtagccctgc tcgtcaagga
     7081 aatcgataag gatgtggacg caggcggctt cttggtcgga aagggggtgt tcgcacgctt
     7141 gcgcgtgcag gtattgcttg aaatcttctt cgccggcgat gttggacagc atatcttcgc
     7201 cttcgtctcc gccgatttga cgcgcgggcg cggtgtaatg gctgaattcg gcatcggaaa
     7261 attcatccgt ttctttgcgt tcgagcaggg ggttgtccga cggtcagttt tcgacctcgc
     7321 gttcaagttc gatacccggc atctgcaata cgcgcaaaga ttgttgcagc cgctggtcga
     7381 gctgctgggt ctgtttgagc tttattccga ttaaggtaat gataatgcgg tgaaaattgt
     7441 tattttcagc ctgtcggc
//
LOCUS       DAXDSD010000097         7398 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXDSD010000097 DAXDSD010000000
VERSION     DAXDSD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.097"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(61..633)
                     /locus_tag="ACFB2Q_001756"
     CDS             complement(61..633)
                     /locus_tag="ACFB2Q_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFC0918730.1"
                     /translation="MQPEQDVRLDGTDTGIEKMATQVGGANSDEASPCFPISEVELVG
                     EEAAKFRFALNHALCQTHFVSGKCLHAGDINQIMSLAQNALIGRGYTTTRILAAPQDL
                     NSGKLQLTLMPGYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLEN
                     LKCLPTAEADLQIVPVEREPNQSDVVVQWR"
     gene            complement(962..7036)
                     /locus_tag="ACFB2Q_001757"
                     /pseudo
     CDS             complement(962..7036)
                     /locus_tag="ACFB2Q_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            7237..7398
                     /locus_tag="ACFB2Q_001758"
                     /pseudo
     CDS             7237..7398
                     /locus_tag="ACFB2Q_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1587 a         1707 c         1771 g         2333 t
ORIGIN      
        1 tcctgtcgcc tcactacccg aattatccat ccccacactc acacagtagg gcagcagacg
       61 ttaccgccat tgcaccacga catcactttg gtttggttct ctctctacgg gaacgatttg
      121 gagatcggct tccgcagtcg ggagacattt gagattttcc agtccttgtt ccaaatcacg
      181 cagattcaac agatcgttcg agcgggtggg aaatttgttt tggaatgctg caatacgtcc
      241 tgcatgggtt tgatcatcgt tggaccgatc gattcgtatg gagcgcagat agcccggcat
      301 cagggttaat tgaagcttgc cactattcaa atcctgtggc gcagccaaga tacgggtcgt
      361 ggtatatccc ctgccgatca aagcattttg tgctaaggac atgatttgat taatgtcgcc
      421 cgcatgcaga cacttgccgg aaacaaaatg tgtttggcac aaggcatggt tgagcgcaaa
      481 ccggaattta gccgcttctt cacccaccaa ttccacctca gaaataggaa agcaggggct
      541 tgcctcatcc gaatttgcac cgcctacctg tgttgccatc ttctctatgc ccgtatcggt
      601 accatccaaa cgaacatctt gttccggctg catctgttca cgcaattgct tctcacgttg
      661 acgttggaga atatcctgtt gttgtgaaac agcttcaggt gtgggggcgg ctgatgttag
      721 ggtggggagg cagccatcaa tgataggact aacagtgata agggataggg atgaaataca
      781 tttttggagg aaaagaaagg tttcatagga ttggtctaag taatgtaagc gggcaattat
      841 agcaatgcac aaaaacaaac aaaaagataa aaaaactcca taaatgaaaa tttaatcaca
      901 tgtttatagg atatgaaaaa gaggaagcga aatctgaagc cgtctgaaaa caaccccaca
      961 cagcatcggc ggacgagttt ccctcagctg ctccggcagc cgtacccagc atccttgtaa
     1021 tcgccgaaac ggtctctttt tcctccgcat tcaggctgcc gccgtctcct ttgccgtaca
     1081 gccatttgcc gatgattggg gcggccgctt ccgacccgcc cgcacccaat gctcctgccg
     1141 gggcattgtt gcctcatgct gcggcaaccg ctgctcctaa taccgcgtgg gcaagaacgt
     1201 gagcggtttc ttgactggcg gttagtttgc cattcgcgtt ttgaccggct aaatctttaa
     1261 agtgctgtcc aatcgcatac gatacgtctg gcgatgcggt agccgcagcg atgcccgctc
     1321 cgctttgggt cggctcagct aaacctgagg ctaacatgtt gagaatgact ttgccttgtt
     1381 gccaattatc tgcttttgct gccgcatctt gagcttcatg ggctttgcgt ttggcagttt
     1441 ccatatcgcc attggctaat gcctcggctg ctgctgtttc ggctgcttct ttgtctgctt
     1501 tgagttggtc taaatgttgg ttaatctcgg tattggtttg ttgaacattt ttactaaaat
     1561 cttggctgac ggttctttgt aaatccagtt cactttgcac tctatctttg tcaaatatgt
     1621 ttttcaggct gcccgaatgt cgttcggcgg tgtctgtggt tacgtttgta tcaatatcgg
     1681 ctttggtttg tgccgctatt ttgcctgtca gcctgatttg tgcggcttcg tctgtgattt
     1741 gaatgttttt gggtattgat gccgcttttt gtgatgctgc tttgactgtc gctgtcgctg
     1801 ccgtacccta ctgatgaact tgcgccgttt ttatcggcta cgcttgtcag gtgtttgtct
     1861 tgaggtttat tttttgcgcc ctgtcccagt gttttgccgc ttacggcaac gctggcaccc
     1921 aatccaaaac tttttccttc gtattggctg taattttgaa tatcactgcc ggcgagtgtg
     1981 cctgtgctga atcggttttt acccttgtct tttgcgcttt ggctgctggt gatgatgccg
     2041 cctttgaggc ctgtatggtt tccgaccttg atttgatagc cgtcttctcc ggcataaata
     2101 ccgctttgct cggttaccga agcatggtcg gctcggattt tgctttggct gtaatcgcca
     2161 ctggcactga agccataacc tacggtaact tgtgcaccgg cgttttgttg tttgctttga
     2221 taagtttctc tatcttgtac actttgaatg cttaggtttt tggcattgac ttgtacgcct
     2281 ttgccgcgta cttgcgcgcc tttgatgatg gtatcgccac cactttggat aagggtttgg
     2341 ctgcctttgt cgccaatatg gctatggcgg tgggttacgc tatcgccatt gccgtagcct
     2401 ttgccgacat tgccgcctgc ggcaacgcct aatgaccagc ctccttgtcc gaatgatacg
     2461 gcagcacctg cgttccagcc tgctgatttg ttttggctgc gttcggtatt gctttgctcg
     2521 gctgattgca gatggatttg cttatcggca atcaggccgg ttcctgctct gcctgaaaca
     2581 cctgagcctg tgatgttgat attggattgt tcgccgcacc ttcggcaata agggtagttt
     2641 tgccgcctgc ttgaatttga ctcgcttggg cttgattggc ttgaacttgg gtggtttgtc
     2701 ggttttgctg ttcgccgtag gttatggaga tgctgacttg tttggcattg gttgtaccat
     2761 tggctaagtt ttgtgcgcct ttgcctgttt gataggcctg ccaacctgca ttggcagccg
     2821 ccatggcatt aacgcggtcg tttttgcttt gtccgacttg tttgctgctt tgtgtgctac
     2881 ggcaatcgct tgttgtgcca aatcggtaac gggcgaactg aatgccaccg ttaagccttt
     2941 ttgttcgtag gtttgggtgg ttttgctgtt taattggttt tgtgctgcgc caatatccat
     3001 actttgcgtg ctgataaggt tgttgccctc agggctggaa acgttgctgc cggtttgttc
     3061 gtagtgtttg cttgcaacaa tggtggtatc gcctttcagg ctgcctacgg tactgcctgt
     3121 atgttcgttg ctttgggatt ggttttcttg tgtgtttgtc ttgctgccaa tagtgaagcc
     3181 gatacctgca ctcatcaatc ctgatttttg ggtttgatga taggtttcgc tttggctttg
     3241 agtttgggtt gtaccaatgc gaacatgatt gcctgcttga atccgggtgc cattatcgga
     3301 aataacatta ctgccaagga tgttggcatc gtttcctgcc tgcaatacaa cttgcttgcc
     3361 ttcaaaggtg ctgctttgag cagtttcgtg atgactttgg gctttatcgg taatgactaa
     3421 tttattaccg ccgccgcttc tgcctgtatg tttggacgca tcatcaactt ggccggcatg
     3481 gatgcctgag ctgatagtaa tgtcattttt agcatacacg gcaagtgtgc cttttgcgct
     3541 gccgacttcg gcagctttgg cattgagatt attccctgac aatagggtaa catcgccttt
     3601 tgtttgaatg ctgctgccga cttcgttcgt tgaacctcgg atggtatggt tatcggcatc
     3661 aaaatggatt tcttgatatt tgccggtttg taccgtatcc aggttaatgt cgcgtcctgc
     3721 ctgcagccgg gtttgccctt gatctgattg attgctgatt tgaccggcaa tgatgttgat
     3781 gtctttgcct gcctgcgctg ctaaaacacc tttttctttg cctgtgatat aaatacctgc
     3841 cattcggtct aggtaggtgc tgctaccttg tgcattttga ctgctcttgg ccgtgctttg
     3901 gttgttgatg ttgttacccg cattgagcaa taatgtctgt tcggcagaaa gaatgccgcc
     3961 aatattattg atgtcttgtg tggccgtaac cgctgatttt tgcgcatgaa tacgcccacc
     4021 gatattgtct agcgtatcgg tattgataat aagcgcattg cgccctgcaa tcgtgcctga
     4081 gtttttcagg ctgcctgaaa cattgatttg tgtattgctg cctgacaaca atgcaccttt
     4141 accgtctatg ccgccatttt taacgcgtac ataaacctgt ggcatcaata cggtttgtgt
     4201 gccgccatca ggaagtttaa cttctttttg taccaaccaa acaatatcgc tggtcagttg
     4261 cgctgcttgc tcggcactta atgcaatgcc aacgctgaga ttcatcgaac gtgccgcagt
     4321 cgcgccatta tccattaagg ctttaaattg ttcttcgtcg ttttgataac cgtctaaacg
     4381 acgatgccct gtcagctctg cgatttgttc attgattaaa cgttgctcgt aataaccatc
     4441 acccaaacgt ttatgtaaat tgtttgggtc tagtttgagg ctgcccagca tatagtcact
     4501 acccaaccat tgacggtagt tggcaaagcg tggatcggtt tcaacaagat agcctttatt
     4561 ggcaggattg ataatgtata agctgctgcc gggtaatggg gtaaaagaat tgggcgtata
     4621 gggtagcgaa ataccgttgc ttttcgcagt acggatatta tcccggttac tttgtggcaa
     4681 ctcagtgcct tggctgggcg catgacggct taatgctttg ctatgcgatt cataggcaaa
     4741 tgaacccagt gaaatgtcgc gtgtgatttc ctccggcaaa gtataatttt gttcacgatg
     4801 ccctgtttca tcatgtcctt tacgacgcgc acgccagtag ttgtgcaact taccattttc
     4861 gctgaagact ttcttctcgc caaaggtttg ctcgttatgc aaaccgtctt tttctgtttg
     4921 cacaagcaaa ttgccgccgg cgatgattcg gctgtcgctg ttgaagactg ctttgctatc
     4981 aataatcaaa tcgctacctg caatgatttt agcaggcgca gttccggtta cttgagtttc
     5041 ttgcgttact ttttcataat cgtatttatg ccaattttca tgcgccactc catcaggggt
     5101 gcgtaagtgg tctgattcat tgttgtagac aaaccagcct aattcatgtt gcgtgccttc
     5161 tcgcaataat tcgtgtcgtc caaatgcttc gtaatcaaca atacgctcgc gccctgtttc
     5221 taccaactgc gttttcaaat gctcattggt attgtgcagc ttttctacac ctaaacgcat
     5281 tttgcctgca gcttcaatga ttgcgccggc attgtgtatc ctttgggctt tgcctgtggc
     5341 ttggtcattg gtatctaatg cgccgccaac cgccatatcg ttaccgctgt aaatcagact
     5401 gttttcacgg ttatttaatt gttcaatgcc taaattcagg ttttcgcgtg ccgcaatggc
     5461 ggcacctgta ccgttttcat cttgattgtc taagcgggta gccgcaatag cgatattgtc
     5521 gccataaatc cgacctgtac cgatattatt catttgcccg gcttggattt tggtttgttg
     5581 tccgtcaatc aagcctctat tggttaaatt gtgctgcgtg ccaatgtctg tcgtaccgcc
     5641 ggattgaatg ttgccttgta ctgcattatc aaggttattt gctttaatcc gaatgcgttt
     5701 tcctgcttgc aaggtatgtg aatttttcag gctgcctcgt gtactgagcg acaattcatt
     5761 gcccgccacg atcttgcgtt ctacataaaa atcatcttgt aacgcaatat ccagtttatt
     5821 atcagcggca agtgtgccgt tgttggataa cgattttgcc tgaatggcaa catcacggcc
     5881 tgattgtatc gtgccattcg tattatcaat gacaacggta gattgctgac catcgtgaat
     5941 aatcagttgt tgattggtcg ctatttcgcc attttgattg ttcaggctgc ctgaaacggc
     6001 taaatcctct gttcctgccg acaataattt gccagtgttt tgaatcgatt gactgtgaat
     6061 ttgagtgcct tgttgcgata ccgccgtacc gctgttttca aaggcttgac tgcggatatt
     6121 gactttgtgt tccgctgtat tattcatatc ttgcacattg gcggcagcca tcgtgccact
     6181 attgaccaaa cggccatttg catcaatcgc cacattaccg gaagaagcaa acaactgccc
     6241 ttgattacga atgcctgctt gttcggccgt actgatcaag gtgattttgt tggcatacaa
     6301 gaatacgtgt gaaatcggta tcacgggcat ccaaaccgtg tccggcgatt acagcattgc
     6361 cttgccttat cttaaagccg ctaaagtctc ctgcttgata ttgcggttgg cctgtcgtca
     6421 aagtggcacg ggaagcattg ataaaaccac caccattgac tgcaatccct gccggattgg
     6481 caataacgac ttctgcacgt cgtccaccca cttcaatata gccattcagt tgtgaaggat
     6541 ggctgctgtt gatttggttt acaaccacac gtgcttcgcc ccttgtcaac caaggattgc
     6601 cttgaatcca accgcctagc tgtgtttggg tgttgctgcg actgttgttt aaaatcgccc
     6661 cgcgattacc cacatcaaac tgggcatatt gattaacaga aacccctgcc gaagtagggg
     6721 tttgaatatt gacttgcggt atgccgttac ctgtttgcag aatcgtggct tgttgggttt
     6781 taggagcagc tttatcagta ataatgccgt cagcaaaagc aatattgacc gtacccaaag
     6841 ccaaacataa agaaaagcct aatgcagaaa aacaaaaggc tttggaatga gtaggaatga
     6901 aagaaacgga tttcacataa acgctgcccg aaccactatc ggcacagctt ttaccttcgc
     6961 gcttggtggt ttcggcaaca gctaccacag caccgcgttt gcggttgaaa atcacacgat
     7021 agagggtttt gttcatgatt ttagttattt gatttttata gagttattac aaacattgga
     7081 cagtctgggc attctaggac aaagattttg gtaagtcaat taccgccatt tttttattaa
     7141 attaggggac gtaccggata acggctaaaa atcccattta ggttagaata agccaatgag
     7201 aaaagccgtc taagccggta taaacaaaat aaactcattg ggctatttgt cgcaggcgta
     7261 actgcaagaa cagcggcaga gttggtaggc gttaataaaa ataccgcagc ctatgatttt
     7321 catcgtttac gatgactgat ttatcaaaac ggtccgcatt tagaaatgtt tgatggcgaa
     7381 gtagaagcag atgaaagt
//
LOCUS       DAXDSD010000098         7173 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXDSD010000098 DAXDSD010000000
VERSION     DAXDSD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.098"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            232..2373
                     /gene="fetA"
                     /locus_tag="ACFB2Q_001759"
                     /gene_synonym="frpB"
     CDS             232..2373
                     /gene="fetA"
                     /locus_tag="ACFB2Q_001759"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC0918731.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNNGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVGDTKSRINITR
                     QAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSEFEIKDKEKATNEEKKK
                     NRANEKIVYAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSSS
                     SLNPSFGVIWQPREHWNFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARNT
                     EIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNTGYIKNHGYELGASY
                     RTGGLTAKVGVSRSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFKNPNLEIGW
                     RGRYVQKATGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLNVNLSVNNV
                     FDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
     gene            2525..3490
                     /locus_tag="ACFB2Q_001760"
     CDS             2525..3490
                     /locus_tag="ACFB2Q_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC0918732.1"
                     /translation="MLRLTTLTLCTVLALGACSPQNSDPAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVVLVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            3508..3699
                     /locus_tag="ACFB2Q_001761"
     CDS             3508..3699
                     /locus_tag="ACFB2Q_001761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918733.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            3718..4686
                     /locus_tag="ACFB2Q_001762"
     CDS             3718..4686
                     /locus_tag="ACFB2Q_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC0918734.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGSSMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     KNEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLMGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            4676..5650
                     /locus_tag="ACFB2Q_001763"
     CDS             4676..5650
                     /locus_tag="ACFB2Q_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC0918735.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTVNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPHMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALVLLVSAAVVWRERYRSDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            5695..6321
                     /locus_tag="ACFB2Q_001764"
     CDS             5695..6321
                     /locus_tag="ACFB2Q_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC0918736.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            6405..7163
                     /locus_tag="ACFB2Q_001765"
     CDS             6405..7163
                     /locus_tag="ACFB2Q_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC0918737.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
BASE COUNT         1670 a         2166 c         1834 g         1503 t
ORIGIN      
        1 tgtactgcct gcggcttcgc cgccttgtcc tgatttttgt taatccacta tacatttccg
       61 acaaaacctg tcaacaaaaa acaacgcttc gcaaataaaa acgataatca gctttacaca
      121 accccccccc ccgctaatat aaacaaaaat aattattatt attttttctt atcctgccaa
      181 accttaacgg tttggcttaa cttcccttca tacactcaaa aggacgaaca aatgaacgcc
      241 ccgtttttcc gcctcagcct gctctcgctc acacttgccg ccggctttgc ccacgcggca
      301 gaaaataatg ccaatgtcgc attggatacc gttaccgtaa aaggcgaccg ccaaggcagc
      361 aaaatccgta ccaacatcgt tacgcttcaa caaaaagacg aaagcaccgc aaccgatatg
      421 cgcgaactct taaaagaaga gccctccatc gatttcggcg gcggcaacgg cacgtcccaa
      481 ttcctgacgc tgcgcggtat gggtcagaac tctgtcgaca tcaaggtgga caacgcctat
      541 tccgacagcc aaatccttta ccaccaaggc agatttattg tcgatcccgc tttggttaaa
      601 gtcgtttccg tacagaaagg cgcgggttcc gcctctgccg gtatcggcgc gaccaacggc
      661 gcgatcatcg ccaaaaccgt cgatgcccaa gacctgctca aaggcttgga taaaaactgg
      721 ggcgtgcgcc tcaacagcgg ctttgccggc aacaacggcg taagctacgg cgcaagcgta
      781 ttcggaaaag agggcaactt cgacggcttg ttctcttaca accgcaacga tgaaaaagat
      841 tacgaagccg gcaaaggttt ccgcaatgtc aacggcggca aaaccgtacc gtacagcgcg
      901 ctggacaaac gcagctacct cgccaaaatc ggaacaacct tcggcgacgg cgaccaccgc
      961 atcgtgttga gccatatgaa agaccaacac cggggcatcc gcactgtgcg tgaagagttt
     1021 gccgtcggcg acacaaaatc acggataaat attacacgcc aagcccctgc ctaccgcgaa
     1081 accacccaat ccaacaccaa tttggcgtac accggcaaag atttgggctt tgtcgaaaaa
     1141 ctggatgcca acgcctatgt gttggaaaag aaacgctatt ccgccgatga caaagataac
     1201 ggctacgcag gcaatgtaaa aggccccaac catacccgaa ttaccactcg gggtatgaac
     1261 ttcaacttcg acagccgcct tgccgaacaa accctgttga aatacggcat caactaccgc
     1321 catcaggaaa tcaaaccgca agcgtttttg aactcggaat ttgaaataaa agataaagaa
     1381 aaagcaacta atgaagagaa aaagaagaac cgtgcaaatg aaaaaattgt ttacgcctac
     1441 aaactttcca acccgaccaa aaccgatacc ggcgcgtata tcgaagccat tcacgagatt
     1501 gacggcttta ccctgaccgg cgggctgcgt tacgaccgct tcaaggtaaa aacccacgac
     1561 ggcaaaaccg tttcaagcag cagcctcaac ccgagtttcg gcgtgatttg gcagccgcgc
     1621 gaacactgga acttcagcgc gagccacaac tacgccagcc gcagcccgcg cctgtatgac
     1681 gcgctgcaaa cccacggcaa gcgcggcatc atctcgattg ccgacggcac caaagccgaa
     1741 cgcgcgcgca ataccgaaat cggcttcaac tacaacgacg gcacgtttgc cgcaaacggc
     1801 agctacttcc ggcagaccat caaagacgcg cttgccaatc cgcaaaaccg ccacgactcc
     1861 gtcgccgtcc gcgaagccgt caacaccggc tacatcaaaa accacggtta cgaattgggc
     1921 gcgtcctacc gcaccggcgg cctgaccgcc aaagtcggcg taagccgcag caaaccgcgc
     1981 ttttacgata cccatcctaa aaaactgttg agcgcgaacc ccgagtttgg cgcacaaacc
     2041 ggccgcactt ggacggcctc ccttgcctac cgcttcaaaa acccgaatct ggaaatcggc
     2101 tggcgcggac gctatgttca aaaagctacg ggttcgatat tggcggcagg gcaaaaagac
     2161 cgcgacggca aattggaaaa cgttgtacgc caaggtttcg gtgtgaacga cgtcttcgcc
     2221 aactggaaac cattgggcaa agacacgctc aatgtcaatc tttcggttaa caacgtgttc
     2281 gacaagttct actatccgca cagccaacgc tggaccaata ccctgccggg cgtgggacgt
     2341 gatgtacgcc tgggcgtgaa ctacaagttc taaaacgcac atcccgaaaa aatgccgtct
     2401 gaaagccttt cagacggcat ctgtcctgat aatttgatat agattatcat ttatcctttc
     2461 taaagccgtt ccggtttgtc cgaccggcgg ctttgcccca atatccccat tttggagaca
     2521 ccctatgttg cgtttgacca cattgaccct ctgcaccgtc ctcgccttgg gcgcgtgttc
     2581 gccgcaaaat tccgaccctg ccccacaagc caaagaacag gcggtttccg ccgcacaatc
     2641 cgaaagcgcg tccgttaccg tcaaaaccgc gcgcggcgac gttcaaatac cgcaaaaccc
     2701 cgaacgcatc gccgtttacg atttgggtat gctcgacacc ttgagcaaac tgggcgtgaa
     2761 aaccggtttg tccgtcgata aaaaccgcct gccgtattta gaggaatatt tcaaaacgac
     2821 aaaacctgcc ggaactttgt tcgagccgga ttacgaaacg ctcaatgcct acaagccgca
     2881 gctcatcatc atcggcagcc gcgccgccaa ggcgtttgac aaattgaacg aaatcgcgcc
     2941 gaccatcgag atgaccgccg acaccgccaa cctcaaagaa agtgccaaag agcgcatcga
     3001 cgcactggcg caaatcttcg gcaaacaggc ggaagccgac aagctgaagg cggaaatcga
     3061 cgcgtctttt gaagccgcga aaaccgccgc gcaaggcaaa ggcaaaggtt tggtggtttt
     3121 ggtcaacggc ggcaagatgt ccgccttcgg cccgtcttca cgactgggcg gctggctgca
     3181 caaagacatc ggcgttcccg ctgtcgatga atcaattaaa gaaggcagcc acggccagcc
     3241 tatcagcttc gaatacttga aagagaaaaa tcccgactgg ctgtttgtcc tcgaccgcag
     3301 cgcggccatc ggcgaagagg gtcaggcggc gaaagacgtg ttgaacaatc cgctggttgc
     3361 cgaaacgacc gcatggaaaa aaggacaggt cgtttacctc gttcccgaaa cttatttggc
     3421 agccggcggc gcgcaagagc tgctgaacgc gagcaaacag gttgcggatg cgttcaacgc
     3481 cgcgaaataa tgaaacggcg gcattcgatg ccgtctgaaa cacggatgca aaccgcttcc
     3541 tgtgtttcag acggcattgc ccgatacggg cgttatcgcg ggaatggcgg caagcgtttt
     3601 gattggaact tgccccatcc tctgcccgtc cggaggtttc aaacaaggct ttccgctccg
     3661 acggttcgga ctgccttgtt tgaatctcct acgccgtaac gcttttccct tctgtttatg
     3721 actgccaaac ctttttccct caacctggcc aacctcctgc tgccggcggt gttgtttgcc
     3781 gtcagcctgt cggtcggcgt tgccgatttc cgctggtcgg atgtgttttc gctgtccgac
     3841 agccaacagg tcatgttcat cagccgcctg ccgcgcacgt ttgcgattgt gctgacgggc
     3901 tcgtcgatgg cggtggcggg gatgattatg cagattctga tgcgcaaccg ttttgtcgag
     3961 ccttctatgg cgggtgcggg ccaaagtgcg gctttgggtt tgcttctgat gtccctgctg
     4021 ctgcctgccg cgccgctgcc ggtcaaaatg tcggttgccg ccgttgccgc gctgatcggg
     4081 atgttggtct ttatgctgct gatccgccgc ctgccgccga cggcgcaact gatggtgccg
     4141 ctggtgggga ttattttcgg cggcgtggtt gaggcggtgg cgacgtttat cgcgtataaa
     4201 aacgaaatgc tgcaaatgct cggcgtgtgg cagcagggcg atttttcgag cgtgctgctg
     4261 gggcggtacg agctgctttg gattacgggc ggtttggcgg tgtttgccta cctgattgcc
     4321 gaccggctga cgattttggg gctgggcgag acggtcagcg tgaatttggg tttgaaccgg
     4381 acggcggtgt tgtggtcggg tttgattatt gtggcactga ttacatcgct ggtcattgta
     4441 acggtcggca atattccgtt tatcgggctg gtcgtgccga atatcgtcag ccgcctgatg
     4501 ggcgacaggc tgcgccaaag cctgcctgcg gtcgccctct tgggcgcgtc tttggtttta
     4561 ttgtgcgaca ttatcggacg cgtgattgtg tttccgtttg aaattccggt ctctacggtt
     4621 tttggtgtgt tgggtacggc tttgtttttg tggcttttat taaggaaacc tgcccatgcc
     4681 gtctgaaaaa aatatcggtt ttatggcagg aagcagccgt ccgttgcggg tcgcctttgc
     4741 gctgttgctg gtttcctgca tcctgtttat gacggtcaac gtcaaaggcg attgggactt
     4801 tgtcttgcac ctgcgcctga ccaagcttgc cgcgctgctg atggtcgcct atgcggtcgg
     4861 cgtgtccact caactcttcc aaacgctgac caacaacccg attctgaccc cttcgatttt
     4921 gggtttcgat tcgctgtatg tgtttttgca gaccttgctg gtgtttacgt tcggcggcgt
     4981 gggctataca tccctgccgt tgacgggcaa attcggcttt gaactggttg ttatgatggg
     5041 cggctcgctg ctgctgtttt acacgctcat ccgtcagggc gggcgcgatt tgccgcacat
     5101 gattttaatc ggcgtgattt tcgggatttt gttccgcagc ctttcctcgc tgctttcgcg
     5161 catgatagac cccgaagaat ttaccgccgc gcaggcgaat atgtttgccg gattcaatac
     5221 cgtccgcagc gagcttttag gcataggcgc gctggtcctg ctcgtcagcg cggcggtcgt
     5281 ttggcgcgaa cgctaccgct cggacgtaca ccttttgggg cgcgaccaag ccgtcaattt
     5341 gggcatcagc tacacgcgca acaccttatg gatactgctt tggattgccg cattggtggc
     5401 gacggcgacc gccgtggtcg gcccggtaag ctttttcggg cttctcgccg cctcgcttgc
     5461 caaccacttt tccccgtccg tgcgccattc cgtccgcctg ccgatgacgg tttgcgtcgg
     5521 cggcatcctc ttggtcggcg gacaaaccgt attcgaacac ttcttgggca tgaaggcggt
     5581 attaagcgtg gtggtcgaat ttgcgggcgg actcgttttc ctctatctcg ttttaaaaca
     5641 caaaaaatga cggatgccgt ctgaacggca gcccgccccg aaaggacaaa ccatatgacg
     5701 caagaacgtt tacccgaatt tttcgaccgc gccccgacgc tgaccgtaca agacccgctt
     5761 gccgcattcc tcggcgcggc cgaaaacggc atcctcactt accgctacgc cgatgccgtg
     5821 cgcctgtgcg gacattcctg cccgaccgtc gcgggcgcgt acctgatggt tatcaaaggc
     5881 ctgaaagcac tttacggcga agagctgccc gaacgcggcg gcatcgaagc cgccatgcag
     5941 ggaacgcgcg acgaaggcac ggtcggcgta accgcgtccg tcgtccaact ccttaccggc
     6001 gcagcccccg aaaccggctt cggaggcatc ggaatgcagg gacgcttcgc ccgccgcaac
     6061 ctcttatcct ttggtgcagg cgaaatcaac ggcacactga ccctgcgccg caaagacaac
     6121 ggcaaaaccg ttgccgtcag cctcaacgcc gccctgcaac cctttgcacc tcaaatgcgc
     6181 gacatcatgc ccaaagccgt cggcggcagc gcaagcgcaa aagaactcga acgcttcgga
     6241 caactttggc aggcacgcgt taaagcattt ttgaccgaat cggcggacga cccgcagttc
     6301 gtcatcgtcc gcgaactgtg aacgttcaga cggcattccg aattccaaat gccgtccgaa
     6361 cccccgccaa acaacaacaa acctacgccc gacaagcatc cgccatgatt accatccgca
     6421 acgtcagcta ccgcatcggc acacacccca tcctcgacaa cgtcagcctc gacatccccg
     6481 aaggcggcat taccgcgcta atcggcccca acggtgcggg caaatccacc ctgttttcct
     6541 ttatggcgcg gctgcgcccg cttgaaagcg gcagcatcgc ctaccgaggc aaaaaccttg
     6601 ccggcacccc caccgccgaa ctcgcccgca cgctctccat cctcacccaa gaaaacagca
     6661 tcatgagccg catcaccgtg cgcgacctgc tgatgttcgg ccgttacccc taccatcaag
     6721 gcagaccgac tgccgaatgc cgccgtatcg ttaacggtgc aatcgaagaa ttccacctgc
     6781 aggacctctc cgaccgctac ctgaccgaac tctccggcgg acaacgccaa cgcgccatga
     6841 ttgcgatggt gttctgccaa agcaccgact acgtcctttt ggacgaaccg ctgaacaacc
     6901 tcgatatgta tcacgcccgc tcgctcatgc agattctgca aaagctcacg cacgaacaca
     6961 aacgcaccac cgtcgccgta ttgcacgaca tcaaccaggc cgccgcctac gccgaccacg
     7021 tcgtcgccat gaaaaacggc aaagccgccc tgaccggcac acccgaagaa gtgttcaccg
     7081 tacacaacat caaagaactg ttcgacatgg acgtggacgt actcgactac gaaggcaaaa
     7141 aactcatcgt ccaccacatc tgaagctcaa aaa
//
LOCUS       DAXDSD010000099         7007 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXDSD010000099 DAXDSD010000000
VERSION     DAXDSD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.099"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(19..1785)
                     /locus_tag="ACFB2Q_001766"
     CDS             complement(19..1785)
                     /locus_tag="ACFB2Q_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFC0918738.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYFLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGKKVLVGKNPWEANGARVATYDAEGNEVWVSVPEVVFDEMGFKADAAVIGAEYK
                     APADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGGKIDPYKHQEDTLAASNTL
                     FMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGDWKQAVSVILKHYPEGVTE
                     GRLEEAFERTRTRCRLKLLKTG"
     gene            complement(2477..2659)
                     /locus_tag="ACFB2Q_001767"
     CDS             complement(2477..2659)
                     /locus_tag="ACFB2Q_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918739.1"
                     /translation="MATEILGHTVGVTANELAIHSMEVMDKFSGGEAYNETVWIERGR
                     FADAKRWKGCLSRDAR"
     gene            complement(2696..2980)
                     /locus_tag="ACFB2Q_001768"
     CDS             complement(2696..2980)
                     /locus_tag="ACFB2Q_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFC0918740.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            complement(2967..3197)
                     /locus_tag="ACFB2Q_001769"
     CDS             complement(2967..3197)
                     /locus_tag="ACFB2Q_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC0918741.1"
                     /translation="MEKPLNFKPIPYPQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKFGLKEDADGDE"
     gene            3469..3849
                     /locus_tag="ACFB2Q_001770"
     CDS             3469..3849
                     /locus_tag="ACFB2Q_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC0918742.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            4083..5123
                     /locus_tag="ACFB2Q_001771"
     CDS             4083..5123
                     /locus_tag="ACFB2Q_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFC0918743.1"
                     /translation="MKKIITHRIYPKNENRERVEPKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTTYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            5120..5494
                     /locus_tag="ACFB2Q_001772"
     CDS             5120..5494
                     /locus_tag="ACFB2Q_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918744.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            5491..6129
                     /locus_tag="ACFB2Q_001773"
     CDS             5491..6129
                     /locus_tag="ACFB2Q_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918745.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAEREVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            6249..6536
                     /locus_tag="ACFB2Q_001774"
     CDS             6249..6536
                     /locus_tag="ACFB2Q_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918746.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            6610..6831
                     /locus_tag="ACFB2Q_001775"
     CDS             6610..6831
                     /locus_tag="ACFB2Q_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918747.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            6824..>7007
                     /locus_tag="ACFB2Q_001776"
     CDS             6824..>7007
                     /locus_tag="ACFB2Q_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFC0918748.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCH"
BASE COUNT         1724 a         1777 c         1635 g         1871 t
ORIGIN      
        1 gctgttttcg gttgaaacct aaccggtttt caaaagtttc agacggcatc ttgtccgggt
       61 gcgttcaaac gcctcttcca gcctgccctc ggtcacacct tccgggtagt gtttgaggat
      121 gaccgacact gcctgtttcc agtcgccgcc gtctgcctcg acgcggggtt ttaagcgttt
      181 ggcgatttcg accttgctca atacctgctc ggcgacctcc atcttgttgt aatccatctg
      241 ttgcccctgt ttgggcataa agagcgtatt gcttgcggca agcgtatctt cttgatgttt
      301 gtacgggtcg attttgccgc cgaacgggac tgccttgcct ttgcgtttgg cggctgccgc
      361 ctccagcgtt tccgcaccca tcgccagctt gtccagctct ttgcgatgcc gttgcgcgtc
      421 tgtatcggcc ggggctttgt attccgcccc gatgaccgcg gcatcagcct tgaagcccat
      481 ctcgtcaaaa actacttcgg gtacggatac ccaaacctcg ttaccctccg cgtcataagt
      541 ggcgacccgc gccccgtttg cctcccaagg attcttaccg accaaaactt tcttaccgac
      601 caaaatcccc ttgatgcctt ttacgctata tacccgtccg ccaaagcgga tttccaaatc
      661 cgccgagact ttcgcctctt tcggcgcgct gacggcaagc tctcggcaat attcggcagg
      721 cggcggcagg atgagctgct cgggtttgat tttgttccac gcctgatagc gggtcatgcc
      781 gtggcggctg tgtttttgcg taccgttgta gtaacgcatc cagcgttccg ataaagcatt
      841 gagctggtcg atgtcgtgta cctcggtaaa gcgcaacccg ctctcaaatg ccgtttccac
      901 aatatcgttg gctttttcca cttgcccctt ggcacgcgga ttgcccggct tgttgatttg
      961 cacatgcaca tccaagggct tgcacaaatt tttaaacgcc gccgaagtat tcgcgctgcc
     1021 cgggtcaagc atgaccatgc gcggtacggt ccggaacggg tctttgccga tgtctttttt
     1081 cgcctgcatc atgtagatga aaaaatcaca gaggttcgcg ctggtttcgc cgccgaagta
     1141 ataacgcacc gaaatcgtgc cggaggcatg gtctgtcccc gtgtaccgcc aaacgcggtc
     1201 gttttcgatt ttgacgacgt ttttcggctt gtttttataa aactcctctt ccttcatcac
     1261 ccgcagcccc gtatccttgc cctgacgcgg caggaaatac aaaacgcaca aactcgggtc
     1321 gatttgccaa caatggttcg ggtgttccga tttcatgcgg ctgacggggt cgggctgcaa
     1381 aagctggtcg gaatgcagct tgtattcccg taaagcccgg gtaatggtgt tttcagaaag
     1441 ggggatgact tccccggttt ccccgtcaat ccgcgccgcc tcgattttcc cgttggcgcg
     1501 cagcatttcc accgcctgcc gcaccggcat caaccgcttg ccgttgcgcc tcatcgcctc
     1561 cgccaaaacc gccgaaatca atttggcttc ttccggttta agctccgtct tgcccgcatc
     1621 gctgcgccgt ttgcgcgtcg gcttgacgct gaccgcctcc agcttgcggt atagcgtggc
     1681 aaggctgatg cccaattcct gcgcctgctg cttaagatat gcagagcgtg cgccgcgccc
     1741 cattgcttcc gcctgatttt cgactgcttt aagacgctca atcattgccg gattcatcgc
     1801 cttctcccgt ttcaccgccc aaccattccg gcacattgtc tgccggtgct tcggtcggta
     1861 gggcatagct ttcgcgcagt tgctcgcagt ccaaaataat ttgattgagc gtgccgacca
     1921 tcttcgcgcg gtggtcaaat ccatgcgcct cgccgtgcgc cgccatctgc tcgaacatat
     1981 cgcgcaatcg gctgatttgc gagcggatac cgacttccaa gctgtcaagc tgccaagctg
     2041 catcgtcagc tcgatgccca catccgcagg tttaggctct ttgacacccg tctgcttttt
     2101 cgacagcttt tccgccagct catcgacctt tttattttta tcggcaatca ccttatcttt
     2161 cgcttccgcc gtttcacggc tttcgctcag ggcgacgcgc agctccttaa tagtcatacg
     2221 gtcgacatcg tcaaaagtgt tgccgttgac ctcaccgcct tcggcaagct ccaacaaagt
     2281 gtcgtcgtct tcgaccagca gctcgagcag tttggatttg cccaacgtca tcagcttagg
     2341 ctgcgcttgt ttcattttcg ggtcgataaa acggagggtg gcattcatca gtctttggga
     2401 ttcccgccgt ccgaggccaa ctctttttca gcgatttcgg caaaccgccc atgcggcgta
     2461 tgctctttga tgatgatcag cgcgcgtccc ggctcaaaca tcccttccat cgtttggcgt
     2521 ccgcaaatcg tccgcgctca atccataccg tctcgttgta ggcctcgccg cctgaaaact
     2581 tgtccataac ctccatactg tggatagcca attcgtttgc cgttacgccg accgtatgtc
     2641 ctaaaatttc tgttgccatt tcttttgctc ctcaaatgcg acgtcgtcgt cgcatttagt
     2701 aaattcggtt ttccaattct tgcaagcgcg cattcagccg ctcttgctgt tttctgaacc
     2761 gctctgcgat ttgtagggtt ttgatgccgt aggcgtagtt gccgttttca agcttgatga
     2821 ccaatcccga tgcaaccaaa tcatcaatat ccctgctgac ttgtgatggc gtcagcccca
     2881 ggccgtctga aagctcctta ttgctgatgc cgattaccgg atgcgcgtcc atcgccaaaa
     2941 acacccgcaa cagccgctgc gcctttttac tcgtcgccat ccgcatcctc cttcagccca
     3001 aattttaccg ccgcttcgtg gcttttgccg aaattgcctt tcagcttgcc gcgcaagggg
     3061 tgttccaccg cggtgcgctc caaattgaaa tgtttcgccc ggtgcgtctt gcacacgccg
     3121 ttgcgcttaa accacacggc cgtgctgccg cgcgtttgcg gatagggaat aggcttgaaa
     3181 tttaaaggtt tttccatatt atttatgcct ttcgtgtggt ataatgtgtt tagttttagg
     3241 cctctttgca cgataaacat ccggtctgtt gtgcagcagc agggtcttgg cttgttttaa
     3301 aataaacaag ctttttcccg tagccgggtg tatgtgatcc atcatgagct gtcgcgcatg
     3361 agccttcaaa gtgttcattt tttcgtcctt tttcgtgatg atttagggtg tttcatgtct
     3421 tgatgtgtga attatgagaa aaatatttct cttttcaagg aaatttaagt ggaaaaaact
     3481 tctctttttg gtaagcgatt gaaagaagaa agaattaaat taggcttgaa ccaagcagaa
     3541 gctgctgaaa aatgtggttt ttctcgtgag atgtggggaa agtgggagcg tggtgaaaat
     3601 cgcccatcaa gtgaaaaatt attctctttt tctaaaatcg gcatcgatat tgattacgtc
     3661 atgcacggca gacgcggcga aacagccgcc atgccgtctg aatccttgag tgctgaagaa
     3721 aaagaactgc ttgccctgtt ccgtcagctc ggcagcggca gccgcaaaga gcttgctgac
     3781 tacgccgcct ttaagctggt ggtagagaaa aaagcccaaa ctgcgcttgg taaagtgagc
     3841 aacggataaa aaatgccgtc tgaagatttc agacggcatt tgggacactc tgacagtcgg
     3901 gctgctccgg tgttctgaaa cagatattaa gcaataggag cggccgtgca ttttaaagcg
     3961 tattaaaaac caccccgata ttcaggtcat cggccaagtg gttcagattt caaaagacct
     4021 taattaactt acaataggaa aaatgatgac agaaacgaat tactcttttg aagggctggt
     4081 aattgaaaaa aattattacc catcgtattt atccgaaaaa tgaaaataga gagcgtgtag
     4141 aacctaaaat cagtacacat ttgattgatt tgcctattac ggcaagaaga acattggaaa
     4201 ccaggctgac caaagctttg ggtaataaat ctcatggcat tgaaatgtct gttgtcaata
     4261 cggctgaaaa tagttttttt cagatagcgg cagccataca actcaaagag gaagcagagt
     4321 ttattgagga ttccgcacaa tttgcccaca tgttgaccga tgcacaactg aatacaaatg
     4381 caccgggtgg tattttgttg gttttaaaag gtagggttgg agataccggt aagccgtttt
     4441 tatgtgtaat taaggctgaa cctcaagatg ggttccgaac caaagaagag gatgacttta
     4501 tcacgattga attcttagaa gaattattac tgaccgattc agcaagatta ttcaagatag
     4561 gttttttggt ggctgaaaca gtaaggccgc tagagcaaat acaatctggg aattatcgag
     4621 cttttttgta tgaccatctg atgacacaaa cggaaactag accggcagct tcctatttct
     4681 atcaagtatt cttgggtatg agtatagctg cttcttcccg taaattgacg caggattttt
     4741 ttgagtggac acgcaatttt atcgataact ctgatttaag tgatgatgca aaattagatg
     4801 cgcatgaagc attgcgcgtt acattgaaaa gtgcggaagc aaccattagt gtaaataatt
     4861 ttgcccaaaa tcatttacct caagaaaaac gaacaactta tacagaattt atggtggaaa
     4921 aggattttcc tcaaaatgcc gtaagtaaag atattgaata tattaaaact cgtttacgca
     4981 aacggaggtc ttacggattt agtaacggtg tagttatctt gactcctccc gagcatactc
     5041 aggactatat ggaaattgcg ccaacggaag atggggaata tactgttgtc ctaattaaag
     5101 gacagttaca acaacaaaaa tgagtgatat tgatgatttc aaaagctatt tagaaagcca
     5161 ccaagcggca ttttccgctt ggggtagatt tgtggctgaa gaaattcaaa atcaattatc
     5221 caatgtcatt tctcctgttc cggttgccaa ttttctaaaa attgaagcaa agcctggagt
     5281 caaagagatt tcttcagctc tagccaagat tggccgaaaa aattacactt cacctcaaac
     5341 tcaaatgact gacttggtcg gcgtacgttt tgtggcattg cttgcggaac atattcaaat
     5401 agtttgtgaa atcattgaat cctcatctca gtggaatgct aaagtttcga aagattttgc
     5461 agatgaaatt cagcaaaatc ccaaagcaat ttgactatac aatccaaaca ttacgagatt
     5521 cgtcctaaac aagcattcat tacacccgaa aatgtcagca tccctgctga tttgtgctgt
     5581 gaagttcagg tgcgttcttt actccaacat gcttatgcag agctggttca tgacaatata
     5641 tacaagcctg acggcaatgt tccaaagcaa gcagagcgcg aagtcgcgaa gagcatggct
     5701 ttgatggaaa ctacagatga cttatttagt cgtactctgg caattttgaa agaagcaaat
     5761 caaccacaag aagagcttct tccccaatta tcacagcttt atcaaaagga aatagggctt
     5821 gttcctgaag ttgataaaaa aaccaatatg atttttctag aaacatttca atccagcatt
     5881 tcccaatcaa gtattctttc tgacatccgt tcacttttga atgagaagaa atatattgcc
     5941 aagcggataa aagaaaatgc tgaagaaatg tattttttca gtcagccagc agctttgttg
     6001 gtctattggc tgattgagaa agttggtgca gatgaagtat ggaaaaaatg gcctttgcct
     6061 gcatacaaca aaaacctgaa attcatttgt actgatttgg ataagcaacc atcccatgaa
     6121 ctcttttaac cccctttaaa agcccattca gacggccttt cctaattatg ccttaatcaa
     6181 acgcggcata gcggcagttc cgcagctggc ggaaatcaaa aaatccgcaa atcaggaagg
     6241 gggcggcaat gattgaattt gtccgagcca aaaaacggct gctttgggca tttgtacttt
     6301 tgcttgcgtg ggcgtgcggc taccggtacg ctgccgacaa ggccgaagcg gaacaaaccg
     6361 ccctgattgc cgcctatcgg cattcttcta tggttgcggc ggaacaatac gccttgcagc
     6421 ttaaaaaagc gcaggacgaa aggcagcggt ggtacgactt ttcccaaaaa caaagcacgg
     6481 atttggcggc cgccctgagc gaattggaca aaacgcgcaa caccttacag gagtaaaccc
     6541 gtgaagcggc agacaaagac ggcaaccgtt ttaacggcct tggcacgaac agcctgcacc
     6601 tctacaaccg tgccttcgga taccccgatt aaaaccgttg ccgtcgcgga aattccgccc
     6661 gttccttccg gactgctggt cgaatacgaa cgccccgagc gtccggccgg cggctctccc
     6721 gaacaacttt taaaccacgc cgtccgctat ggcggatact accgaaagct cgaaatccaa
     6781 attgagggct ggcagaactg gcacacgaaa ggccgtctga aacatgactg attttgccga
     6841 ccgcgcatcc gaacgcgaag ccgaatttct ggtggaagcc ttggcgaaac accaaccgcc
     6901 gtctgaaaac accgccggct tcagccattg cgaagactgc ggcgacccga taccggaagc
     6961 aaagcgaaaa gccgtccgag gctgtacgcg ctgtgttgtc tgccacg
//
LOCUS       DAXDSD010000100         6954 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXDSD010000100 DAXDSD010000000
VERSION     DAXDSD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.100"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..597
                     /locus_tag="ACFB2Q_001777"
     CDS             <1..597
                     /locus_tag="ACFB2Q_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC0918749.1"
                     /translation="KGKTGSLTALPIIETQAGDVSAFVPTNVISITDGQIFLETDLFN
                     AGIRPAINAGISVSRVGGAAQTKVIKKLGGGIRLALAQYRELAAFSQFASDLDEATRK
                     QLEHGEVVTELMKQKQFSTLNTAEMALTLWAINNGSYSDVPVAKALAFESEFLSFVRT
                     QHPEVLEAVNASGAMSDESEKTLEAAMKSFKSSYAYQA"
     gene            622..1497
                     /gene="atpG"
                     /locus_tag="ACFB2Q_001778"
     CDS             622..1497
                     /gene="atpG"
                     /locus_tag="ACFB2Q_001778"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC0918750.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDKIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKPPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            1535..2932
                     /gene="atpD"
                     /locus_tag="ACFB2Q_001779"
     CDS             1535..2932
                     /gene="atpD"
                     /locus_tag="ACFB2Q_001779"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC0918751.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            2943..3365
                     /locus_tag="ACFB2Q_001780"
     CDS             2943..3365
                     /locus_tag="ACFB2Q_001780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC0918752.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            3537..4433
                     /gene="glyQ"
                     /locus_tag="ACFB2Q_001781"
     CDS             3537..4433
                     /gene="glyQ"
                     /locus_tag="ACFB2Q_001781"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC0918753.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENADLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            4550..6613
                     /gene="glyS"
                     /locus_tag="ACFB2Q_001782"
     CDS             4550..6613
                     /gene="glyS"
                     /locus_tag="ACFB2Q_001782"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC0918754.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGTPTKALEGFARGAGAK
                     IEDLTIIHDGRQDVYAYEYVQTGRPLGGLLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEIIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAMPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAGRAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFRTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            6677..>6954
                     /locus_tag="ACFB2Q_001783"
     CDS             6677..>6954
                     /locus_tag="ACFB2Q_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC0918755.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSGGGDILRA"
BASE COUNT         1771 a         1808 c         1819 g         1556 t
ORIGIN      
        1 aaaggcaaaa ccggttctct gaccgcgttg ccgattatcg aaacccaagc gggcgacgta
       61 tctgctttcg ttccgactaa cgtcatttcg attaccgacg gtcagatttt cttggaaacc
      121 gacctcttca acgccggtat ccgtcctgca atcaatgccg gtatttccgt atcccgcgta
      181 ggcggtgctg cacaaaccaa agtgattaaa aagctgggtg gcggtatccg tttggcgttg
      241 gcacaatatc gtgaattggc ggcgttctcg caatttgcat ccgatttgga tgaagctacg
      301 cgcaaacagt tggagcatgg cgaagtcgta accgaactga tgaaacagaa acagttcagt
      361 acgttgaata cggctgaaat ggctttgacc ctttgggcaa tcaacaacgg ttcgtattct
      421 gatgttccgg ttgccaaagc tctagctttt gaatctgaat ttttgagctt tgtccgtacc
      481 caacatccgg aagttttgga agccgtcaat gcttcaggtg caatgtccga cgagagcgag
      541 aaaacgcttg aagcagccat gaaatccttc aaatcttctt acgcctatca ggcataaggc
      601 ttgaagtgaa aggagtctga aatggcagta ggaaaagaga ttctcaccaa aatccgcagt
      661 gttcagaata cccaaaagat cactaaagcg atgcaaatgg tgtcaacctc taaaatgcgg
      721 aagactcagg aacggatgcg tttggcgcgt ccgtatgccg aaaaagtgcg tatggtgatg
      781 agccatcttg cgcaaaccaa taccgatcat ggtattccgt tactggaatc tcatcgggaa
      841 atcagacgtg tcggttttat tttgattacg tctgataagg gtttgtgtgg cggtttgaac
      901 gccaacgtgc tgaaaaagtt tttggcacaa gttcaagagt atcggaatca aggtattgaa
      961 gaggaaatcg tatgccttgg cagtaaaggt ctgatggcgt gtcagagcat tggtctgaat
     1021 gtggttgcca gtgccgtaaa tttgggcgat accccaaaaa tggaaatgct gctcggacct
     1081 ttgacagaac ttttccaacg gtatgagaaa catgaaattg acaaaatcca tctggtgtat
     1141 tcgggttttg tcaataccat gcgtcaagaa ccgaggatgg aagtattgct gcctatcggt
     1201 gagaacgtga ttggcgattc agctcccaaa ccaccgttca gctgggaata ccgctacgaa
     1261 ccgactgcac ttgcagtgtt ggaatatctg gttcgccgct atttagagtc tgtggtttat
     1321 caggcgttga gcgacaatat ggcatccgaa caggcagccc gcatggttgc catgaaagct
     1381 gcaacagaca atgcaggcaa tgccatcaaa gagttgcgtc tggtatataa caaatcgcgt
     1441 caagctgcga ttaccacgga attgtcagaa attgtagcag gtgcggcggc cgtctgatgc
     1501 cgtctgaatc ctgtacggaa attaggatac gataatgagc caaggcaaaa tcgtacaaat
     1561 tatcggtgcg gttgttgacg tggaatttcc acgcgacatg attccgcgcg tttacgacgc
     1621 tttgaaatta gacgaaaacg gtctgacttt ggaagtccaa cagcttttgg gtgatggcgt
     1681 agtccgtacc atcgctatgg gcagctcgga cggtttgaaa cgcggcatga ctgtgagcaa
     1741 tactggttcg cccattactg tgccggtagg taaaggtacg ttgggacgca ttgtcgatgt
     1801 attgggaact cctgttgacg aggcaggtcc aattgatacc gacaagagtc gtgccatcca
     1861 ccaagccgct cctaagtttg acgaactgtc ttccacaacc gaattgctcg aaacgggcat
     1921 taaagtgatt gacttgctgt gtccgtttgc caaaggcggt aaagtaggtc tgttcggcgg
     1981 tgccggtgtg ggtaaaaccg tgaacatgat ggaattgatc aacaacatcg ccaaagcgca
     2041 cagcggcttg tccgtgttct caggcgtggg tgagcgtacc cgcgaaggta acgacttcta
     2101 ccacgagatg aaagattcca acgtattgga taaagtagcc atggtgtatg gccaaatgaa
     2161 cgaacctcca ggcaaccgtc tgcgcgttgc tttgaccggt ttgactatgg ctgaatactt
     2221 ccgtgacgaa aaagacgaaa acggtaaagg tcgtgacgta ttgttcttcg ttgacaacat
     2281 ctaccgttac actctggccg gtaccgaagt atccgcactg ttgggccgta tgccttctgc
     2341 agtgggttac caaccgacat tggctgaaga aatgggtcgt ttgcaagagc gtattacctc
     2401 tacccaaacc ggttccatta cttccatcca agccgtatat gtacctgcgg atgacttgac
     2461 tgacccgtct ccggcgacaa ctttcgccca cttggacgcg accgtcgtat tgagccgtga
     2521 tattgcctct ttgggtattt acccggcagt tgacccgctt gactctactt cgcgccaatt
     2581 ggatccgatg gtattgggtc aagagcacta cgacgtagcg cgcggtgtac agtctactct
     2641 gcaaaaatac aaagaattgc gcgacatcat cgccattctg ggtatggacg aattgtctga
     2701 cgaagacaaa ctggctgtaa tgcgtgcccg taaaatccaa cgcttcctgt ctcaaccgtt
     2761 ccacgttgcc gaagtgttta caggttctcc aggcaaatat gtcgccctgc gcgataccat
     2821 tgccggcttc aaagccatct tgaacggcga atacgatcat ctgcccgaac aggcattcta
     2881 tatggtcggc agcatcgaag aagcggttga gaaagcgaaa accttaaact gaggaggtcg
     2941 gcatgagcat catgcaagtt gaggtggtaa gtggcgagca gaaaatctat tcaggcgagg
     3001 caacatttat cgttgttccg actgtacagg gcgaactcgg tatttatccg cgacacgagc
     3061 cgattatgag tttggtgcgg ccgggggctt tgcgtttgac cgttccgggc gaggataaag
     3121 aggttttggt tgctgtttcc ggcggtgttt tggaagtaca gcctgataaa gtaactgtct
     3181 tggcggatgt tgccgtccgc agtgcagaga tggatcaggc acgtgcggaa gaggcgaaaa
     3241 aagccgcaga agcgggtatt tcccaagcta aagacgataa ggctttggcg gaagcacata
     3301 aagcattggc tgctgcgatt gcacagctca aaactttgga ctatatccgt tcgcacaaga
     3361 aataaacttg tttggtttga aaaagcacga ccgtttggcc gtgcttttta ttttccggat
     3421 ttaaattaaa aaattatcca tcccctggtt tcaaatcagg ctgtcttcga gataaatcta
     3481 ttcaagcggc tatggaaaca gtaaaataaa acctttattt tatcgaacaa taagccatgc
     3541 ttaccttcca acaaatcatc ttcaaactgc aaaccttctg ggcagacaaa ggctgcaccg
     3601 tcatccaacc cttcgacatg gaagtcggtg ccggcacatc ccatcccgcc acctgcctgc
     3661 gcgcgctcgg ccccgagcct tggtttgccg cctacgtcca acccagccgc cgccccaaag
     3721 acggccgcta cggcgacaac cccaaccgcc tgcaacacta ttaccaattc caagtcgccc
     3781 tcaaacccgc ccccgccaat atccaagacc tctatctcga ctccctgcgc gaattgggca
     3841 tcgatcccaa agtccacgac atccgctttg tcgaagacga ctgggaaaac cccaccctcg
     3901 gtgcgtgggg tttgggctgg gaagtctggc tcaacggcat ggaagtaacc cagtttacct
     3961 atttccaaca agtcggcggt atcgattgca cgcccgtact cggcgaaatt acctacggca
     4021 tcgaacgctt ggcgatgtac ctgcaaggcg tggaaaacgt ctatgacctc gtttgggcaa
     4081 aaacgccgga cggcaatacc gtaagctacg gcgacgtgta ccatcaaaac gaagtcgagc
     4141 aatctaccta caacttcgaa tacagcgatg ccgactggct gctgcgccag ttcaacgact
     4201 acgaagcgca agccaaacgc ctgttcgccg aagaaaacgc cgaccttgcc ttacctgcct
     4261 acgagctggt cctcaaagcg gggcatacgt tcaacctttt agacgcacgc ggcgcgattt
     4321 ccgtaaccga gcgggcaact tatattggac gtattcgtgc attgagccgc gccgtggcgc
     4381 aaaaatatat cgaaagccgt gagaaactgg gcttcccgtt gattaaaaaa taacgatacg
     4441 tcgtctgaag caggttttca ggcgaccttt attgcgtatc cattaataca ggccgtacgg
     4501 catatgcagg ctttataagc atggcctacg gacaaacaga aagtctcaca tgacaaccca
     4561 aaccctttta atcgaactcc ttaccgaaga actcccgcca aaagccctga ataatctggg
     4621 caaccatttt gccgcttccg ttgccgaagg cttggaaaaa gcgcaactgg ttgacggcgc
     4681 ggccgaattt acggcttatg cttcgccgcg ccgtttggcg gttcaagtca aaaacgtcaa
     4741 agctgttcag gccgatcaga aaatcgtgaa aaaaggccct gccgtggcga atgccgtaaa
     4801 agacggtacg ccgaccaagg ctttggaagg ttttgcgcgc ggggcggggg cgaaaatcga
     4861 agacctgacc atcatccacg acggcaggca ggacgtgtac gcctacgaat acgtccaaac
     4921 cggcagaccg ttgggcggac ttttggaaga cattatcaat caagctgtta agaagctgcc
     4981 gattccgaaa gtaatgcgtt ggggcagcag cacgtttacc ttcgtgcgcc ctgttcacgg
     5041 gctgattgtg ctgcacggcg gcgatgtcgt gaacgtcagc gttttgggcc tgcaaagcgg
     5101 caatcaaacc ttgggacacc gctttctttc cgacggcgaa atcatcattg aaaacgccga
     5161 cagctacgcc gcacaaatgc gcgggcaagg caaagtcgtc gcttcgtttg ccgggcgcaa
     5221 agccgcgatt cagacggcat tggaagggca ggcacgccgt ctgaacgcga ccgttgccgc
     5281 cgatgaagcc ctgttggacg aagtaaccgc gctggtcgaa tggcctgttg ttttggaagc
     5341 cggttttgag gaacacttcc tcgccatgcc tcaagaatgc ttgattttga cgatgcagca
     5401 aaaccaaaaa tacttcccgc tgctcgacca aaacggcaag ctgatgaacc gcttcctgct
     5461 ggtttccaac ctgcaaaccg aagacccgtc gcacatcatc cgaggcaacg aacgcgtatt
     5521 gcgcgcgcgc ctgtctgatg ccgagttctt ctacaaacaa gaccaaaaag cgaccttgga
     5581 aagccgcctg cccaagctgg cgaacgtggt gtaccacaac aaaatcggtt cgcaggccga
     5641 acgcatcgaa cgcctgcaaa gcatcgccgc ccatatcgcc aaagcattgg gcgcggatgc
     5701 cgcggcagcc gggcgcgccg cgcgtcttgc caaagccgac ttggtaactg aaatggtcgg
     5761 cgagttcccc gaactgcaag gcacgatggg caaatactac gcccgcttgg acggcgaaac
     5821 cgaagaaatc gccgaagcta ttgagcaaca ctaccaaccg cgttttgccg gcgacaagct
     5881 gcccgaaagc aaaattgccg ccgccgtggc acttgccgac aaactggaaa ccctggtcgg
     5941 catttggggc atcggtttga ttcctaccgg cgacaaagac ccctacgccc tgcgccgcgc
     6001 cgccttgggt attttgcgga tgctgatgca gtacggtttg gacgtgaacg aactgattca
     6061 gacggcattc gacagcttcc cgcaaggttt gctcaacgag aaaacgccgt ctgaaaccgc
     6121 cgactttatg caggcgcgcc ttgccgtgtt gctgcaaaac gattatccgc aagacatcgt
     6181 tgccgccgta ctcgccaaac agccgcgccg tttggacgat ttgaccgcca aactgcaggc
     6241 cgtcgccgta ttcaaacaac tgcccgaagc cgccgcgctc gccgccgcca acaaacgcgt
     6301 gcaaaacctg ctgaaaaaag ccgatgccgc gttgggcgca gttaacgaaa gcttgctgca
     6361 acaggacgaa gaaaaagccc tgtacgctgc cgcgcaaggt ttgcagccga aaatcgccgc
     6421 cgccgtcgcc gaaggcaatt tccgaaccgc cttgtccgaa ctggcttccg tcaagccgca
     6481 ggttgatgcc ttcttcgacg gcgtgatggt gatggcggaa gatgccgccg taaaacaaaa
     6541 ccgcctgaac ctgctgaacc gcttggcaga gcagatgaac gcggtggccg acatcgcgct
     6601 tttgggcgag taaccgttgt acagtccaaa tgccgtctga agccttcagg cggcatcaaa
     6661 ttatcgggag agtaaattgc agcctttagt cagcgtattg atttgcgcct acaacgtaga
     6721 aaaatatttt gcccaatcat tagccgccgt cgtgaatcag acttggcgca acttggatat
     6781 tttgattgtc gatgacggct cgacagacgg cacacttgcc attgccaagg attttcaaaa
     6841 gcgggacagc cgtatcaaaa tccttgcaca agctcaaaat tccggcctga ttccctcttt
     6901 aaacatcggg ctggacgaat tggcaaagtc gggggggggg gatatattgc gcgc
//
LOCUS       DAXDSD010000101         6930 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXDSD010000101 DAXDSD010000000
VERSION     DAXDSD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.101"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            120..488
                     /gene="rplN"
                     /locus_tag="ACFB2Q_001784"
     CDS             120..488
                     /gene="rplN"
                     /locus_tag="ACFB2Q_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC0918756.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            500..823
                     /gene="rplX"
                     /locus_tag="ACFB2Q_001785"
     CDS             500..823
                     /gene="rplX"
                     /locus_tag="ACFB2Q_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC0918757.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            833..1372
                     /gene="rplE"
                     /locus_tag="ACFB2Q_001786"
     CDS             833..1372
                     /gene="rplE"
                     /locus_tag="ACFB2Q_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC0918758.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            1375..1680
                     /gene="rpsN"
                     /locus_tag="ACFB2Q_001787"
     CDS             1375..1680
                     /gene="rpsN"
                     /locus_tag="ACFB2Q_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC0918759.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            1696..2088
                     /gene="rpsH"
                     /locus_tag="ACFB2Q_001788"
     CDS             1696..2088
                     /gene="rpsH"
                     /locus_tag="ACFB2Q_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC0918760.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            2102..2635
                     /gene="rplF"
                     /locus_tag="ACFB2Q_001789"
     CDS             2102..2635
                     /gene="rplF"
                     /locus_tag="ACFB2Q_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC0918761.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            2649..3002
                     /gene="rplR"
                     /locus_tag="ACFB2Q_001790"
     CDS             2649..3002
                     /gene="rplR"
                     /locus_tag="ACFB2Q_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC0918762.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            3021..3539
                     /gene="rpsE"
                     /locus_tag="ACFB2Q_001791"
     CDS             3021..3539
                     /gene="rpsE"
                     /locus_tag="ACFB2Q_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC0918763.1"
                     /translation="MAKHEIEERGNGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            3532..3717
                     /gene="rpmD"
                     /locus_tag="ACFB2Q_001792"
     CDS             3532..3717
                     /gene="rpmD"
                     /locus_tag="ACFB2Q_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC0918764.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            3719..4153
                     /gene="rplO"
                     /locus_tag="ACFB2Q_001793"
     CDS             3719..4153
                     /gene="rplO"
                     /locus_tag="ACFB2Q_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC0918765.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            4165..5475
                     /gene="secY"
                     /locus_tag="ACFB2Q_001794"
     CDS             4165..5475
                     /gene="secY"
                     /locus_tag="ACFB2Q_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC0918766.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            5480..5698
                     /gene="infA"
                     /locus_tag="ACFB2Q_001795"
     CDS             5480..5698
                     /gene="infA"
                     /locus_tag="ACFB2Q_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC0918767.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            5719..5832
                     /gene="rpmJ"
                     /locus_tag="ACFB2Q_001796"
     CDS             5719..5832
                     /gene="rpmJ"
                     /locus_tag="ACFB2Q_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC0918768.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            5898..6260
                     /gene="rpsM"
                     /locus_tag="ACFB2Q_001797"
     CDS             5898..6260
                     /gene="rpsM"
                     /locus_tag="ACFB2Q_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC0918769.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            6280..6675
                     /gene="rpsK"
                     /locus_tag="ACFB2Q_001798"
     CDS             6280..6675
                     /gene="rpsK"
                     /locus_tag="ACFB2Q_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC0918770.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            6695..>6930
                     /gene="rpsD"
                     /locus_tag="ACFB2Q_001799"
     CDS             6695..>6930
                     /gene="rpsD"
                     /locus_tag="ACFB2Q_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="primary rRNA binding protein; nucleates 30S
                     assembly; involved in translational accuracy with proteins
                     S5 and S12; interacts with protein S5; involved in
                     autogeneously regulating ribosomal proteins by binding to
                     pseudoknot structures in the polycistronic mRNA; interacts
                     with transcription complex and functions similar to
                     protein NusA in antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC0918771.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEA"
BASE COUNT         1957 a         1148 c         1673 g         2152 t
ORIGIN      
        1 tccctttcgg gatccaagac tggtttactt gaaccgcaag gtttcattta ataagcagcg
       61 gctttgctgt aagttatctg aaagtggtaa attaagttgg ttaatttaaa ggtaataata
      121 tgattcaaat gcagaccatc ttagatgtgg ctgataactc tggtgcgcgt cgcgtaatgt
      181 gtatcaaggt attgggcgga tctaagcgtc gctacgcttc tgttggcgat attattaaag
      241 tggcagttaa agatgcggtt ccgcgtggcc gtgtcaaaaa aggcgatgta tataatgcgg
      301 tagttgttcg tactgctaag ggtgtgcgtc gtcctgatgg tgcgttaatt aaattcgata
      361 acaatgccgc cgtgttgctg aataataaac ttgaaccttt gggtactcgt atctttggtc
      421 cggtaacccg tgaattgcgt actgagcgat ttatgaaaat cgtttcattg gcacctgaag
      481 tattataagg aatagcacga tgaataaaat cattaaaggc gatagggttg tagtaattgc
      541 tggtaaggat aaaggtaagc agggtcaagt agttcgagtg ttgggtggta aagttgttgt
      601 tgagggcgtt aatgttgtaa aacgccatca aaaacctaat ccaatgcgtg gcattaaggg
      661 cggtattatt actaaagaaa tgcctttgga tatttctaat atcgcaatcc tgaatccgga
      721 aactaataaa gcggaccgtg ttggtattaa gctgattgaa aatgaaggca aagttaaacg
      781 cgttcgtttc ttcaaatcaa atggctctat cattggagca taaggagata acatggctcg
      841 gttgagagag ttttataaag agacagttgt tcctgaattg gttaaacaat ttggttacaa
      901 atcagtaatg gaagtcccgc gtattgaaaa aatcaccttg aatatgggtg tgggtgaggc
      961 tgttgctgac aaaaaagtta tggaacatgc tgtttccgat ttagagagaa ttgccggtca
     1021 aagaccggtt gttactgttg cccgtaaatc tatcgcaggt tttaaaatcc gtgataacta
     1081 tccggttggt tgcaaagtaa cattgcgtcg tgatcaaatg tttgaattct tggatcgttt
     1141 gattactatt gcattacctc gcgtacgtga cttccgtggt gtgagcggta aatcatttga
     1201 tggccgtggc aattacaata tgggtgttcg tgagcaaatt attttcccgg aaattgaata
     1261 cgataaaatt gatgctttgc gtggtttgaa tattactatt actactacag caaaaaccga
     1321 tgaggaagcg aaagctttat tgtcattgtt taaatttccg ttcaaaggat aatcatggct
     1381 aagaaagcac ttattaatcg tgatctgaaa cgtcaagctt tggctaaaaa atatgcggct
     1441 aaacgcgcgg caattaaagc ggtaatcaat gattcgaatg caactgagga agagcgtttt
     1501 gaggctcgtt tgaggtttca atccattcct cgtaatgcgg cacctgtgcg tcaacgtcgt
     1561 cgttgtgctt tgacaggtcg ccctcgtggt actttccgta aatttggttt gggtcgtatt
     1621 aaaatccgtg aaatcgccat gcgtggcgaa attccgggtg ttgttaaagc cagctggtaa
     1681 taggagtaat taagaatgag tatgcatgat cctatttccg atatgttgac tcgtatccgc
     1741 aatgcgcaac gtgctaataa agcagcagtt gcaatgcctt cttcaaaatt aaagtgtgct
     1801 attgcaaagg tattgaaaga agaaggatat attgaggact tcgcagtttc atctgacgta
     1861 aagtctatat tggaaattca attaaaatac tatgcaggtc gtcctgtaat tgaacaaatc
     1921 aagcgtgtat ctcgccccgg tttgcgtatt tataaagcgt ctagtgagat tccaagtgtt
     1981 atgaatggct tgggtattgc tatcgttagt acttctaaag gtgtaatgac tgatcgtaaa
     2041 gcacgttctc aaggtgttgg tggtgagttg ttatgcattg tagcctagtg gaggaaaaga
     2101 aatgtcacgt gtcgcaaaaa acccagtgac ggttcccgct ggtgtagaag taaaatttgg
     2161 aacagaggca ttggttatta agggtaagaa cggtgaattg tcttttcctt tgcattctga
     2221 tgtagccatt gaatttaatg atggcaaatt gacttttgtt gcgaataata gcagtaaaca
     2281 agcaaatgca atgtctggta ctgctcgcgc attagtcagc aatatggtta aaggtgtttc
     2341 agaaggtttt gagaagaaat tacaattgat aggcgtgggt tatcgtgctc aagcacaagg
     2401 taaaatcttg aatctgtctt tgggtttttc tcatccgatc gtatatgaaa tgcctgaagg
     2461 tgtctccgtt caaactccta gccaaacaga gattgtttta actggctcgg ataaacaagt
     2521 tgttggtcaa gttgcttctg agattcgtgc gttccgtgct cctgagcctt ataaaggtaa
     2581 aggtgttcgc tatgtaggcg aagtagtggt aatgaaagaa gccaagaaaa aataattgag
     2641 gttcactaat ggataaacat acaacccgac tccgtcgtgc acgcaaaacc cgtgctcgta
     2701 ttgcggactt gaaaatggta agattatgtg tgttccgaag caataatcat atttatgctc
     2761 aagtaattag tgctgaaggt gataaagtat tggctcaagc ctctacattg gaagctgagg
     2821 tgcgcggtag tctgaaatct ggaagcaatg ttgaagcagc tgcaatagtt ggtaaacgta
     2881 ttgctgagaa ggctaaagca gcaggtgtag aaaaggttgc ttttgatcgt tcaggtttcc
     2941 aatatcacgg tcgtgtgaag gctttggctg aagctgctcg tgaaaatggt ttaagcttct
     3001 aaatatttgg agactttcag atggcaaaac atgaaattga agaacgcggt aacggtctga
     3061 ttgaaaagat ggtcgcagtt aaccgtgtaa ccaaagtagt taaaggtggt cgcattatgg
     3121 ctttctctgc gctaactgtt gttggtgatg gagatggtcg tattggtatg ggtaaaggta
     3181 agtcaaaaga agtgcctgtt gcagtccaaa aggcgatgga tcaagcacga cgctctatga
     3241 ttaaggtacc attaaaaaat ggtacgatcc atcatgaggt tattggtcga catggtgcta
     3301 ctaaagtatt tatgcagcct gctaaagagg gtagtggcgt aaaagccggt ggacctatgc
     3361 gtttggtttt tgatgctatg ggcattcata atatttccgc caaagtgcac ggatctacta
     3421 acccatataa tatcgtacgt gcaacattag atggtttgtc taagttgcat actcctgctg
     3481 atatcgcagc caaacgtggc ttgacagtgg aagacatttt gggagttaac catggctgaa
     3541 caaaaaaaga ttagggttac attggttaaa agcctgattg gtacaattga atctcatcgt
     3601 gcatgtgcac gcggtttagg tttgcgtcgt cgcgagcata cggtagaggt tttagatacc
     3661 tctgaaaacc gtggtatgat taataaaatc agctacttgt tgaaagtgga gtcttgatat
     3721 gtttttgaat acaattcaac ctgctgttgg tgctacgcat gctggtcgtc gtgttggacg
     3781 cggtattggt agtggtcttg gcaaaacggg tggtcgtggt cataaaggtc aaaagagccg
     3841 gtctggtggg tttcataagg tgggtttcga gggtggtcaa atgcccttgc aacgacgcct
     3901 ccctaaaaga ggttttaaat ctttaacagt atcagctaat gcacagcttc gtttaagtga
     3961 actggaatca attgctgtta atgagattga tattttagtc ttaaagcaag caggtctgat
     4021 tgcatctaca gtctctaatg ttaaagttat tgcttctggt gaaatttcta aggcagttgc
     4081 tttgaagggt attaaagtta ccaaaggtgc gagagctgct atcgaggatg ttggtggtaa
     4141 gattgaaatg taaggtttaa tattgtggct aatcaacaaa cgtcatcagg ttcatccaag
     4201 tttggagatc ttaagaaacg tcttttgttt ctatttggag cattgattgt ttttcgaatt
     4261 ggtgcccata tacccgtacc tggggttgat gctgttgctt tagctaaatt atacgaaagc
     4321 gctggaaacg gcatcctggg aatattgaat atgttttccg gtgggtcgtt agagcgcttt
     4381 agtatatttg caataggaat tatgccatat atttcagctt ctattattgt acagctcgct
     4441 tctgaaattt tgccatcatt gaaggcttta aaaaaagaag gggaggctgg tagaaaggta
     4501 attacgaaat atactaggta tggtactgtt ttgttagcaa ttcttcaaag tctaggtgtt
     4561 gcatctttcg tatttcagca aggaattgtt gtaacaagtt catttgagtt tcatgtttcc
     4621 acggtagttt ctttggtaac gggaaccatg tttcttatgt ggcttgggga gcaaattact
     4681 gaaaggggta tcgggaacgg tatttcttta atcattacgg caggtattgc ttcaggtatt
     4741 ccttcgggta ttgcaaagct ggttacactg acgaaccaag gttctatgag catgcttacg
     4801 gcgttgctta ttgtatttgg tgccttatta ttaatttatt tggttgtata ctttgaaagt
     4861 gcacagcgga agattcctat tcattatgca aaacgccagt ttaatggtag ggcgggtagt
     4921 caaaatacgc atatgccttt caagttgaat atggctggtg ttattccccc aatttttgct
     4981 tccagtatta ttctatttcc atctactctt ttaggttggt ttggttcggc tgatacaaat
     5041 agtgttttgc acaaaatagc tggattgtta caacacggtc aattgctgta tatggcttta
     5101 tttgcagcga cagttatttt cttttgttat ttttatacgg ctttggtttt tagtcctaaa
     5161 gaaatggcag agaatttaaa aaagagtggt gcttttgttc ctgggattag acctggtgag
     5221 cagacctcta ggtatttaga aaaagttgta ttacgtttga cattgtttgg agctctttat
     5281 attacaacta tttgtttaat tccagagttc ttaactacgg ttttaaatgt acctttttat
     5341 ttgggtggca cgtctttgtt gattctagtt gttgtaacga tggattttag tacacaaata
     5401 aattcgtata ggcttactca acagtatgat aagttaatga ctcgttcaga aatgaaatca
     5461 ttttctcgga aatagaatta tggcgaaaga agatactatc caaatgcaag gtgaaattct
     5521 tgaaacttta cctaacgcaa catttaaagt aaaacttgag aatgaccata ttgtattggg
     5581 tcatatttct gggaagatgc ggatgcatta cattcgtatt tctccgggag ataaggtcac
     5641 agtagagctg acaccttatg atttaactag ggctcgaatc gttttcagag caagataaac
     5701 caataaaagg aaaataaaat gcgtgtacaa ccatctgtta agaaaatttg ccgaaattgc
     5761 aagattattc gtcgaaatcg tgtagttcgt gtgatttgta ctgatccccg tcacaaacag
     5821 cgtcaaggtt aatggaatat ttcttttaat gtgattctgt gatatagtga caaaatttgc
     5881 tctaaaaagg aaaaaacatg gctcgtattg caggggtaaa tatccctaat aacgcacaca
     5941 tcgtaattgg tcttcaggct atttacggta ttggtgctac tcgtgctaaa ttgatttgtg
     6001 aggctgcaaa tattgcgcct gatactaaag caaaagattt ggacgagact caattagatg
     6061 ctttgcgtga tcaagttgcc aagtatgaag tagaaggtga tttgcgtcgt gaagtaacta
     6121 tgagtatcaa acgattgatg gacatgggct gctatcgtgg cttccgtcat cgtcgcggct
     6181 taccatgccg cggtcaacgc actcgtacaa atgcgcgtac tcgcaaaggt ccgcgtaaag
     6241 cgattgctgg taagaaataa attttaagga attttattaa tggctaaagc aaacacagct
     6301 tcacgtgtac gtaaaaaagt acgtaaaacc gtgagtgagg gtattgtgca cgttcatgca
     6361 tctttcaaca ataccatcat tacaatcact gaccgtcaag gcaatgcgtt gtcttgggct
     6421 acctctggcg gcgctggttt taaaggttct cgtaaaagta caccatttgc agcacaagtt
     6481 gcagcagaag cagctggtaa agttgcccaa gagtatggcg ttaaaaattt agaggttcgt
     6541 attaaaggtc caggtccagg tcgcgaatcc tctgtacgtg ctttgaatgc tcttggtttc
     6601 aagattacca gcattactga cgttaccccg ttgcctcata acggttgccg tccgcctaaa
     6661 aaacgtcgta tttaatattg gagtgatttg aaacatggca cgttatattg gccctaaatg
     6721 taagttggca cgtcgcgaag gtacggattt gtttttgaag agtgcgcgcc gctctttgga
     6781 ttctaaatgt aaaattgatt ccgctcccgg tcagcatggt gcaaaaaaac cgcgtttgtc
     6841 agactatggt ttgcagttgc gtgaaaaaca aaaaatccgc cgtatttatg gcgtattaga
     6901 acgtcagttc cgtcgttatt tcgcagaagc
//
LOCUS       DAXDSD010000102         6887 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXDSD010000102 DAXDSD010000000
VERSION     DAXDSD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.102"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            29..241
                     /locus_tag="ACFB2Q_001800"
     CDS             29..241
                     /locus_tag="ACFB2Q_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC0918772.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
     gene            426..940
                     /locus_tag="ACFB2Q_001801"
                     /pseudo
     CDS             426..940
                     /locus_tag="ACFB2Q_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
     gene            937..1263
                     /locus_tag="ACFB2Q_001802"
     CDS             937..1263
                     /locus_tag="ACFB2Q_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFC0918773.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAAGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1284..2339
                     /locus_tag="ACFB2Q_001803"
     CDS             1284..2339
                     /locus_tag="ACFB2Q_001803"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFC0918774.1"
                     /translation="MGNSRLSQLPAPAAIEETDFEGIFARKKAALTALCPESIRETVA
                     QTLELESEPLTIDLQQQAYQELLVRNRINEAVKANLLAYAQGSDLDHIAAQYGLSRKT
                     IRAADPDANPPVAAEYETDDAFRARVQAHPEKYAAGPRTAYEAHAIDAHPQITHARAV
                     RRAAGTVEVYIKTQSGTPDETILTAAREYLSAETRRPLCDNVQVTAAQPKDAAVEYSA
                     EYHPAANIQAERQTACEALDNLWRQNAHIGASVALSKIIGALDTPGVKKNHTAQPRRR
                     HRMRQRRIHPNHVHARARINEQHRPREQQPPATRTGKADRTRNCRRLPPTRPRIFTLS
                     RLRKKHRHGRGLGLCRN"
     gene            2224..2718
                     /locus_tag="ACFB2Q_001804"
     CDS             2224..2718
                     /locus_tag="ACFB2Q_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC0918775.1"
                     /translation="MTERETAAVSRQLDPGFLPFHAFARSIGTEEGWDFAETDEARRN
                     LIAGFAEIHARKGTPYAIRALFPILRLGEIQIIERDGEFKWDGSVLFDGSRTFGRRKG
                     DWAEYRIVLTRPVSIRQTARIRAMLAEIAPLRCELTALDYRNHPHRWNGKIRFNGEYG
                     FGTT"
     gene            2758..5009
                     /locus_tag="ACFB2Q_001805"
                     /pseudo
     CDS             2758..5009
                     /locus_tag="ACFB2Q_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5108..5437
                     /locus_tag="ACFB2Q_001806"
     CDS             5108..5437
                     /locus_tag="ACFB2Q_001806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918776.1"
                     /translation="MPRQSCHSSPFPRKRESGTQTRQESIGKNNPTAVIPAQAGIQTH
                     NVKAVYQKKPKPNALDFRLRGNDEELGSDERREQPRKKPPTPSDGIGNKNRTAETARE
                     RIAGRAR"
     gene            complement(5453..5764)
                     /locus_tag="ACFB2Q_001807"
     CDS             complement(5453..5764)
                     /locus_tag="ACFB2Q_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFC0918777.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(5772..6051)
                     /locus_tag="ACFB2Q_001808"
                     /pseudo
     CDS             complement(5772..6051)
                     /locus_tag="ACFB2Q_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6136..6749
                     /locus_tag="ACFB2Q_001809"
                     /pseudo
     CDS             6136..6749
                     /locus_tag="ACFB2Q_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6728..>6887
                     /locus_tag="ACFB2Q_001810"
     CDS             6728..>6887
                     /locus_tag="ACFB2Q_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC0918778.1"
                     /translation="MGAKRRLREYIYHLLVAIDQLFNAITGGAADETLSSRTYRGARL
                     AQQPKTRWK"
BASE COUNT         1858 a         2360 c         1655 g         1014 t
ORIGIN      
        1 tatgcgaaac aaaataaacc gcaacgacat ggaactcggc tacacgcctt acaaccttcg
       61 caccctgcgc aaccgctgca aactgacaca ggccgagttg gcgcaaatcg tcggggtgaa
      121 gcactacata caggtaggcc gctgggaagc cgaacccgat accgaaacgc ggcgggccga
      181 tatgccgctg gaaaaatggc ggcagttcct cgattggata gaaaaaacaa acgccgtctg
      241 aaaccttcag acggcatttt ttacccaccc ccaaacctaa aaaccacaac caagccctac
      301 accgcttgcc cccacctcgc cgccgcccca aaataacctc actctgttgg aagcctgccg
      361 gaaacggcaa aagccgcccc gttccgccat tacggggcgg tttcccacat caagacgaga
      421 cagaaatgac cgaccgccaa atagacaacc tgatcaaacc cgccaccatc gccgacaccg
      481 accccgcgtc cgcgcacaac acggcggcat taccaccgac tggctgccct atatcgtccc
      541 ctttgcaggc ggcgtgtcag tatggcgcat cccaagtgtc ggcgaagcct gcaccatctt
      601 atcccctgcc ggcgaacccg agaacggcgt agttttatgc tgccaagcct ccgaccgata
      661 ccccgccccg tccgccgacc ccgccgaaac cgtcgtccga tttcccgacg gcacgcacat
      721 ccgctacaac cacaacagcg gcgcgatgga attaaaagcc gttacaagcc tgaccatcga
      781 caccccccaa accaccataa ccggacacct gaccgtcaac caaaccacca ccgcccaagg
      841 gctgctgacc taccaaaacg gcatgaacgg ccaaggcggc agcctgtccg agcacaccca
      901 ccccgacgac tccggcggta caacggaaaa accccaatga ccgacgcaga aaacggacgc
      961 gggcaagaca ccctcgccca catcgcccaa tccatccgca acatcctgtt cacaagaata
     1021 ggaacgcggc tgatgcgcga agaatacggc agttttattc ccgacttgat cgatatgccc
     1081 gccggtcacg ccgccatcgc cctcatccac caagccgccg ttaccgccct cgcaagatgg
     1141 aaaccccgca taaccgtccg ccgcatacag gcagacaccg ccgacctcgc cgccggcaaa
     1201 atcaaattaa ccctagacgt aacccttgcc gacggcggcg aacgaaccta ccgcatcaaa
     1261 taataaaaac aacaggataa acaatgggaa acagccgatt aagccaactc cccgcgcccg
     1321 ccgccatcga agaaaccgac tttgagggca tcttcgcgcg caaaaaagcc gccctcaccg
     1381 ccctatgtcc cgaaagcatc cgcgaaaccg tcgcccaaac cctcgaatta gaatcagaac
     1441 ccctaaccat cgacctgcaa cagcaagcct atcaagagct gctcgtccgc aaccgcatca
     1501 acgaagccgt caaagccaac ctcttggcat acgcacaagg cagcgacctc gaccacatcg
     1561 ccgcccaata cggactttca cgcaaaacca tccgcgccgc cgaccccgac gcgaacccgc
     1621 cggttgccgc cgaatacgaa accgacgacg cattccgcgc ccgcgtccaa gcccatcccg
     1681 aaaaatacgc cgccgggccg cgcaccgcat acgaagccca cgccatcgac gcacaccccc
     1741 aaatcacaca cgcccgcgcc gtgcgccgcg ccgccggcac ggtggaggtt tacatcaaaa
     1801 cccaaagcgg cacgcccgac gaaaccattt tgaccgccgc gcgcgaatac ctgtccgccg
     1861 aaacacgccg ccccctttgc gacaacgtgc aagtaaccgc cgcccaaccc aaagacgcgg
     1921 cagtagagta ttccgccgaa taccaccccg ccgccaatat tcaagccgaa cggcagaccg
     1981 cctgcgaagc cttggataac ctatggcggc aaaacgccca catcggcgca tccgtcgccc
     2041 tgtctaaaat catcggcgcg ttagacaccc ccggcgtgaa aaaaaatcac actgcacagc
     2101 cccgccgccg acatcgaatg cggcaacggc gaatacatcc gaatcacgtc cacgctcgag
     2161 cgcgaataaa tgaacagcac cgtccccgcg aacaacagcc ccctgcaaca cgcactggca
     2221 aagctgacag aacgcgaaac tgccgccgtc tcccgccaac tcgaccccgg atttttaccc
     2281 tttcacgcct tcgcaagaag catcggcacg gaagagggct gggactttgc cgaaactgac
     2341 gaagcccgcc gcaacctcat cgcaggcttt gccgaaatcc acgcccgaaa aggcacgccg
     2401 tacgccatcc gcgccctctt ccccatcttg cggctgggcg aaatccaaat tatcgaacgc
     2461 gacggcgagt tcaagtggga cggctcggtc ttgttcgacg gcagccgcac attcggcagg
     2521 cgcaagggtg actgggcgga ataccgcatt gtcttaacgc gccccgtcag catccgccaa
     2581 accgcccgca tccgcgccat gttggcggaa atcgccccct tgcggtgcga acttaccgcg
     2641 ctcgactacc gcaaccatcc ccaccgctgg aacggcaaaa tccgctttaa cggcgaatac
     2701 ggtttcggca cgacataacg cgcccccgaa aatcaacaaa caaaaggaag ccccaaaatg
     2761 gcaaacgcaa ccgaacaaaa ccaattcgac caagccgtcc gcctcatcga acccggcgac
     2821 agcgtcgtcg tcggcccagg cgcgcccgtc aaccaaccac tgcaagccct cgccaaccgc
     2881 acccttttgc tcaaaaacca aaccgaagcc cttcagacgg catccgacac caaagccgcc
     2941 gcaagcaccg ccgtcaacgc aggcgacgga ctgacgggcg gcggcagcct tgcccaaagc
     3001 cgcaccatcg cactgggcgc gcccgggcaa atcaccgcaa caagccaaaa caccgtccca
     3061 aaaaacggcc acacccacgc catcgacacc gcacgcaccg accgcgccgg catcgtccga
     3121 ttagacaatg ccattagcga agccgaagac accgccgcca cccccaaagc cgtcaaaacc
     3181 gcgctcgacc aagcccgcgc cgccgccgcg accgccgacc tcaaagtctc cctctccgac
     3241 aaccaaaccg ttaccggcca aaaaaccttt accgccgaaa cacaattcca aagcggcatc
     3301 cggctgtccg ccaacccgac gcactgaaac ggcggctaca aagcctatat cggcgcggat
     3361 aacgaaaacg cccacatcgt cttcggcgac gacaccctgc gcctgcacgg cgcaaataac
     3421 cgcatttcct acaacaacca cgacatcttc cacaaagcca acaaaccgcg tttcaacgaa
     3481 gacatcgaag gcaaaccgaa cacgctgtcc ggctacggca tcggcaactt caaagtcgaa
     3541 acattccggg gcgatttgaa cagcctcaaa accgacggcg tctattccct gccgacggcg
     3601 gtcggcagct cgaacctgcc cgttgaaaac accgcctgcc atatccaagt catcgccggc
     3661 acgcaacccg gctggtgcag gcagttgggc tatcccgcct acacgtccga cgtgtacgaa
     3721 cgctaccagc ccagcagcgc aaacgacgac tggagcgcgt ggaaaaaact caattcggaa
     3781 ggcatccccg ccggcgcgat cgtgtccttt cccaaaaccg tccgaaaccc cgcaggctat
     3841 ctcaaagccg acggcacgat ctttgcacaa aacaccttct cgacctttac cgcgccctgg
     3901 gcaacacaaa caaactgccc gacctgaccc gtaccgacat cggcatcacc gcgtggtttc
     3961 cgtccgacca aatccagacg ggctggctgg cgtttgacga catccgagag cgcgtaaccg
     4021 aatcggctta tcccgaactt taccgcctgc tgaccgaaaa atacggacgc atccaaaacg
     4081 tcccgcaggc ggaagaccgc tttatccgca acgcgggcaa cggcttggcg gtcggaacga
     4141 agcaggaaga cgaaatcaaa cggcacgtcc acaaagtatt ttcacactgg gcaaaccacc
     4201 cagacgccgc cgccgtcggt tacgaagacc gcaatgaaag gcaaagaagc gcgctcgtat
     4261 cgacttggac ggacggcgac ttaaacgaca acggcttttt aaccccgcga ttggacagca
     4321 aaatggcaac gggcggcgca gaaaaccgcc ccaaagccct ggttttaaaa ctgtgcatca
     4381 aagccgccga caccttgggc gaagccgtgt tccggataaa gtcccacggc gaaaccgcca
     4441 acgtcggcgc actggacgcg tcaaggctgg cgcaaggttt gcaggaaaaa gccgaccgcg
     4501 accacaccca caccaccgcc caaatccaag ggctggacga aaaaatcagc gccgccgttg
     4561 ccgcgcaatt cacacgccaa accatcggcg gcgtggatat tgtcagattc cccgacggca
     4621 caatgataca gaccggcagt tacaggtttg caagaggcgg cagccccata ggaaacgaag
     4681 ttgtcttccc catcgccttt gccgacggca acgtcaaatg cttcgtatcc gaacgccatt
     4741 cgggacgcgt caacggcgaa aggcaacaca actggctgtt tatccgcgca aaaaaccacg
     4801 ccgccgccat tattaccaac tggtacgaaa gcagttgcga ctggatggcc atcggcaaat
     4861 ccgcctcggg aaacgccgcc agccccaccc cgatagtccc cgaaatacct gaaatcgatg
     4921 aagaaccgca aagagagagt ggaagatcat caaccggact ccgaaacccc cgccgccacc
     4981 gaggcctcga cttccccgtg gggtcgtaag actagcgggc ggcagggcag gctgtagaga
     5041 cgggcttcag cccaccgatc caagcaatcc gaccgaaacc ggccgcgccg ccaatcctgc
     5101 gaaacctatg ccccgccaat cctgccactc ttcgccattc ccgcgaaagc gggaatccgg
     5161 aacccaaacg cggcaggaat ctatcggaaa gaataacccg accgccgtca ttcccgcgca
     5221 ggctggaatc cagacccaca acgttaaggc ggtttatcag aaaaaaccga aaccgaacgc
     5281 cctagatttc cgcctgcgcg ggaatgacga agagttgggg agtgacgaaa ggcgggaaca
     5341 accgcgcaaa aagccgccga ctccttcaga cggcatcggc aataaaaacc gcacggccga
     5401 aaccgcgcgg gaaaggatag ccgggcgcgc ccgataagca gcggccgcag ggttaagtcc
     5461 ttgcacctgt tttcatcgaa gacaaaggcg gttcgggaaa taagggcgca atatcaaaag
     5521 ttttgcgttg cctgatttgc cacaaatcat aaccggcctg catattcagc caaaaatcag
     5581 gcgaagtgtt gagcagttgg cttaaacgaa ccgccatctc gggcgtaatc gaagattggg
     5641 cgttgacaat gcgcgataag gcaacccggc tgacacccag gcgtttcgct atatcggtaa
     5701 tgcttgcgcc gttaatatac tcttttaaaa caaggcccgg gtgggcagga ttgtgcattt
     5761 tcatattatt cctagtgata atcctgataa tcgacaactt cgacattgcc gtcgttgaaa
     5821 cgaaaagtca aacgccaatt tccgttgact ttgaaccgcc caatgtcccg acaaactgcc
     5881 cttgagggga tgaagattcc aactcggcac agccatatcc gaaggcgttt gcgcggcatt
     5941 cagcgcggtt agaagcagat tcagtttgac ggaatgcccg gcttggatgc ccgatagcga
     6001 gcctgtttta aaaaagcgtt caagcccttt gtgtttgaaa gaaacaatca tcatcccacc
     6061 ccgtctgtat cgccaaagga tacaaaactt ttatttgatt gcaaagcgac gatatacaaa
     6121 aaaggaaacc ccgaaatgac catctatttc aaaaacggct tttacgacga cacattgggc
     6181 agcgtccccg aaggcgcggt tgccgtcggc ggcgaagagt acgccgccct cttcgcagga
     6241 caggcgcagg gcgggcaaat tgccgccgat tccgacggcc gccccgtttt aaccccgccg
     6301 cgcccgtccg agtaccacga atgggacggc aaaaaatgga aaatcagcaa agccgccgcc
     6361 cgtttcgcca actcccgttt cgccaaacaa aaaaccgcct tggcattcag cctcgcggaa
     6421 aaggcggacg aactcaaaac agcctcttgg cgggctatcc ccaagtggaa atcgacagct
     6481 tttacaggca ggaaaaagaa accctcgcgt ggcaggcgga caacgacgcc ccgaccccga
     6541 tgctggcgca aatcgccgcc gcaaggggca tagaattgga cgttttgatt ggaaaggttg
     6601 tcgaaaaacc cgcccgcctg gccgttgccg tcgacgcgat tatcggaaag cggcagcaac
     6661 ttgaagatag attaaatacc atcgaaaccg cgccagcatt agatgcgtta gaaagggaaa
     6721 tcaaagaatg ggcgctaaac gtcggctgag agaatatatt tatcatctac tagtggccat
     6781 agaccaactg ttcaacgcta taaccggcgg cgcggcagac gaaaccctct caagtcgcac
     6841 ctatcgcggc gcgcggctcg cccaacaacc caaaacccgc tggaaga
//
LOCUS       DAXDSD010000103         6858 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXDSD010000103 DAXDSD010000000
VERSION     DAXDSD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.103"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(954..1637)
                     /locus_tag="ACFB2Q_001811"
     CDS             complement(954..1637)
                     /locus_tag="ACFB2Q_001811"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC0918779.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1786..4089
                     /gene="parC"
                     /locus_tag="ACFB2Q_001812"
     CDS             1786..4089
                     /gene="parC"
                     /locus_tag="ACFB2Q_001812"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC0918780.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDRSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRVVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAVFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4109..5833
                     /locus_tag="ACFB2Q_001813"
     CDS             4109..5833
                     /locus_tag="ACFB2Q_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFC0918781.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYLWLAIACWLIFFSTLNPQWQWQWQALRIPSFSAVADITMIGVLTY
                     LFGGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILD
                     AKTVTNTFVVVAGSYFVAMIASLSVRYIDRAGKLAHENHVAYRRIRGLNQIVLNRVQE
                     AVVVINVEHQTILFNKKAKDLLPMLEIGQHTSLFDPVAILWDKTSSRTFEHYIDTPEL
                     TARIRAVPMNKKQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIR
                     HADGGTLFLDEVADLPLSMQVKLLRAIQEKAVRRIGDATEQPVDVRIVCATHKNLEAL
                     VESGAFRQDLYYRLNVVSLNMPSLREMRENLKLPTPYLLYKHSHNNRPYTLSPAAQQM
                     LLNYSYPGNFRELENILERAVALCVGYTVQIDDLQIQDVHHKPVRTETAVPVADTLPS
                     ETAAAPSRLLPFDPDTMQIQDYLDKIERDIIGQVLKQTEGNRTQAAKRLGISFRSMRY
                     RMERLNID"
     gene            5933..6685
                     /locus_tag="ACFB2Q_001814"
     CDS             5933..6685
                     /locus_tag="ACFB2Q_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC0918782.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVILLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
BASE COUNT         1642 a         2046 c         1685 g         1485 t
ORIGIN      
        1 tattttagta gttttaccat tagaaaaagg atatgaatgg aattgccttg aggtagaagt
       61 actatccgct gctcaatctc tagcaaatat ctattgcaac aaggttcgcc tcatagatgg
      121 tcatgtttgg gaggaataaa tgaaaaagcg tagttggtaa tttttagttg cgcagggtgg
      181 gcttcagccc accgtttttt cttcattttg ggttgttgat tggtgggcta aagcccaccc
      241 tacgattact aaaatcccgt cattcccgcg tagagcgcat catataaggc gggaatccag
      301 acctttcggt gcgaaaactt atcggataaa acggtttccc ctgcatcctg gattcccact
      361 ttcgtgggaa tgacgggata taggtttccc tacggacgtg ttcagattcc tgccttcgcg
      421 ggaataacgg cggagcagtt tctgcttttc caataaatga ccgtaactta aaatcccgtc
      481 atccccgcga aagcaaaaat cctgcctgtc ggatttcggt tttttcggga aactgatgaa
      541 tcgtcattcc cgcgcaggcg ggaatccaga acgcgggacg gcggcaatat tcaaaggttg
      601 tctgaaaatt cagaggttcc agattcccgc cttcgcggga atgacagcgg agcagtttct
      661 gcttttccga taaatgaccg caacctaaaa cccatccttc acacgaaaac agaaaaacaa
      721 aaacctaaaa tcccgtcatt cccgcgataa cagttgcgta attgcgtagg gtgggcttca
      781 gcccaccgtt tcttcatttt tgcttgtcaa ttagtggact gaagcccacc ctgcggttac
      841 tgtttcttct ttttcgatta ttaattggca ggctgaagcc ctccctacgt ttaccgtcaa
      901 aaatgccgtc tgaaatgctc aagccctcag acggcatttt ttatttcgca cccttacagg
      961 taaaatttct cgatgacttt caggttgtcg tctaatttgt acaccagcgg ctggccggtc
     1021 gggatttcca agcccatgat gtcttcgtcg gaaatgccct cgatgtgttt tgccagcgcg
     1081 cgcagggagt tgccgtgcgc cgctaccaag acgcgtttgc cgctcaaaat cgcgggggcg
     1141 atttggtctt cccaaaacgg taatacgcgc tccagcgtta ctttcaggtt ttcgccgtcg
     1201 ggtacgacat cggcaggcag atgggcatag cgacggtctt tgtgtgcgga aaacgcgtcg
     1261 tctttgtcca aaagcggcgg cagggtgtcg tagctgcgcc gccagatgcg gacttgctcg
     1321 tcgccgtatt tttcggcggt ttgttttttg tccaaacctt gcagtcggcc gtagtggcgt
     1381 tcgttcagcc tccatgtttt gatttgcggt acgaacagtt ggtcggattc ttccaaaacg
     1441 atgttgcagg tcttaatcgc gcgggtcagg acggatgtga aggcgatgtc gaactcatag
     1501 ccgttttctt tcagtttttt accggcggcg gcggcctcgg caagcccctg ctcgctcagc
     1561 ttcacgtcgc gccagcctgt aaacaggttt ttcgcgttcc attcgctttg tccgtggcgg
     1621 ataaatacca attccatatc gtctccaatg tgtgaaagtg ggaaagcttt atttataaca
     1681 tattttcaca tttcccgtat ttgattcaga ttcaggcacg gcgcccacta tggtttgcca
     1741 ttttgattta caataatgtc ctttgcttta cattccgcat acacaatgaa tacgcaaccg
     1801 cacgcttccc ataccgattc caacacgctg atgctcggcc gatacgccga acgcgcctat
     1861 ctcgaatacg ccatgagcgt ggtcaaaggc cgcgcgctgc ctgaagtttc agacggccaa
     1921 aagcccgtgc agcggcgcat tttgtttgcc atgcgcgata tgggtttgac ggcgggggcg
     1981 aagccggtga aatcggcgcg cgtggtcggc gagattttgg gtaaatacca tccgcacggc
     2041 gaccgttccg cctatgaggc gatggtgcgc atggctcagg attttacctt gcgctacccc
     2101 ttaatcgacg gcatcggcaa cttcggttcg cgcgacggcg acggggcggc ggcgatgcgt
     2161 tacaccgaag cgcggctgac gccgattgcg gaattgctgt tgtccgaaat caatcagggg
     2221 acggtggatt ttatgccgaa ctacgacggc gcgtttgacg agccgctgca ccttcccgcc
     2281 cgcttgccta tggtgttgct caacggcgcg tcgggcatcg cggtgggtat ggcgaccgag
     2341 attccgtcgc acaatttgaa cgaagtcacg caggcggcga ttgcactgtt gaagaaaccg
     2401 acgctggaaa ccgccgacct gatgcaatat attcctgctc ccgattttgc cggcggcggt
     2461 caaatcatca cgccggcgga cgaattgcgc cgtatttacg aaaccggcaa gggcagcgtg
     2521 cgcgtgcgtg cgcgttatga aatcgagaaa ttggcgcgcg gacagtggcg cgtcatcgta
     2581 accgaactgc cgccgaacgc caattccgcc aaaatccttg ccgaaatcga agagcaaacc
     2641 aacccgaaac cgaaagcggg taaaaagcag ctcaaccaag accggctcaa taccaaaaag
     2701 ctgatgctgg atttaatcga ccgcgtgcgc gacgagtccg acggcgaaca tcccgtgcgc
     2761 cttgtatttg aacccaaatc cagccgcatc gataccgata ccttcatcaa cacgctgatg
     2821 gcgcaaactt cgctggaagg caatgtgtcc atgaacttgg tgatgatggg tttggacaac
     2881 cgccccgcgc agaaaaacct gaaaacgatt ttgcaggaat ggctggattt ccgcgtcgtt
     2941 accgtaacac gccgtctgaa attccgttta aaccaagtgg aaaaacggct gcacatcctc
     3001 gaaggccgtc tgaaagtctt tctgcacatc gacgaagtga ttaaagtcat ccgcgaatcg
     3061 gacgacccga aagccgattt gatggcggtg ttcgggctga ccgaaatcca agccgaagac
     3121 attttggaaa tccgcctgcg tcagctggcg cgtttggaag gtttcaaact cgaaaaagaa
     3181 ttgaacgaat tgcgcgaaga acaaggccgt ctgaacatcc ttttgggcga cgaaaacgaa
     3241 aaacgcaagc tgattatcaa agagatgcag gcggacatga agcagttcgg cgacgcgcgc
     3301 cgcacgctgg tggaagaggc cggacgcgcc gtgctgacac aaaccgccgc cgacgaaccc
     3361 atcacgctga ttttgtcgga aaaaggctgg atacgcagcc gtgccggaca taatctcgat
     3421 ttgagccaaa ccgcgttcaa agaaggcgac cgcctcaaac aaacccttga aggccgcact
     3481 gttttacccg tcgtcatcct cgattcatcg ggcagaacct actcgatcga tgccgccgaa
     3541 atccccggcg gacgcggcga cggcgtaccg gtttcctcct taatcgagtt gcaaaacggc
     3601 gcgaaacccg tcgcgatgtt gacaggattg ccggaacaac attatttatt atcaagcagc
     3661 ggcggctacg gctttatcgc caagctgggc gatatggtcg gacgcgtgaa agcgggcaaa
     3721 gtggtgatga ccgcagacag cggcgaaacc gtcctgccgc cggttgccgt ctatgcctcc
     3781 tcgttcatca accccgactg caaaatcatt gcagccacca gtcaaaaccg cgccctcgcc
     3841 ttccccatcg gcgaattgaa aattatggcg aaaggcaaag gactgcaaat catcggatta
     3901 aacgccggcg aatcgatgac gcataccgcc gtttcttccg agccggaaat cctgattgaa
     3961 agcgaaggca ggcgcggcgc ggcgcacaaa gaccgcctcc ccgtcgccct gattgaggca
     4021 aaacgcggca aaaaaggcag actgttgccc atatcgggca gcctgaaaca gctttcttcc
     4081 cccaaataaa cccggttccg cacatattat ggtgatttcc aacccccgcg aacttgaaaa
     4141 actcaaagac cggattccca atctgatcaa catcatccgc gtcgccatcg ttttcccgct
     4201 gatgattatg cacatcctcg ggctggaaac cggcagccgt gcgaacctgc acgcctcatg
     4261 gacggcgtgg gcgttttact tgtggcttgc cattgcctgc tggctgattt tcttttccac
     4321 cctcaacccg caatggcaat ggcaatggca ggctttgaga ataccgagtt tcagcgccgt
     4381 ggccgacatc acgatgatcg gcgtgctgac ctacctgttc ggcggcatcg attcggggtt
     4441 cggcatcctg atcctgccct tcgtgggcag ctcctgccta ctcagttacg ggcgttatcc
     4501 cctgctctat gccagctatg catccatcct gctgatattc aacgcccttg cagacagcaa
     4561 tatcaatatg tatccgctga ttctggatgc aaaaaccgtc accaatacct tcgtcgtcgt
     4621 agccggctcc tatttcgtcg ctatgattgc ctcgctgtcg gtcagataca tcgaccgtgc
     4681 cggcaaactt gcccatgaaa accacgtcgc ctaccgccgt atcaggggct tgaaccaaat
     4741 cgtgctcaac cgcgttcagg aagcggtcgt cgtcatcaac gtcgagcatc agaccatact
     4801 gttcaataaa aaggcaaaag atctgctccc catgcttgaa atcggacagc atacctccct
     4861 gttcgaccct gtcgccatct tatgggataa aacatcctca cgcaccttcg aacactatat
     4921 cgacacgccc gaactgaccg cccgcatccg cgccgtgccg atgaacaaaa agcagaacaa
     4981 gctgctcatc ctctatatcc gcccgcaaag cgaaattcag gcggaagccc tgtccgtcaa
     5041 acttgccgcg ctcggacaac tgaccgccaa ccttgcccac gaaatccgca acccgatgtc
     5101 cgccatccgc cacgccgacg gcggcacttt atttttggac gaagtggccg acctgcccct
     5161 ttccatgcag gtgaaactct tgcgcgcgat tcaagaaaaa gccgtgcgcc gtatcggcga
     5221 cgcgaccgag cagcccgtcg atgtccgcat cgtctgcgcc acccacaaaa acctcgaagc
     5281 ccttgtcgaa agcggcgcat tccgccaaga cctgtattac cgtctcaatg tcgtcagcct
     5341 caatatgccg tccctgcgtg aaatgcgcga aaatttgaag ctgcccaccc cctacctcct
     5401 gtacaaacac agccacaaca accggcccta cacactctcc cccgccgcgc aacagatgct
     5461 cctgaattac agttatccgg gcaatttccg cgaactcgaa aacatcctcg aacgcgccgt
     5521 cgccctgtgc gtcggatata cagtgcaaat cgacgacctg caaatccaag atgtgcacca
     5581 caaacccgtc cggacggaaa ccgccgtccc cgttgccgat accttgccgt ctgaaacagc
     5641 cgccgcaccg tcccgccttc tcccgttcga ccccgatacc atgcagatac aggactatct
     5701 cgacaaaatc gaacgcgaca tcatcggaca agtcctcaaa caaaccgaag gcaaccgcac
     5761 gcaagccgcc aaacgcttgg gcatcagctt ccgctctatg cgctaccgta tggaacgcct
     5821 caacatagac tgacaaacgg catccgcacc atctccgccc acccgaaaaa atgccgtctg
     5881 aaacggcacg gggaagcggg ttcgccccac gaccgaacgg acacaataca ccatgaccga
     5941 catcctcatt gacgataccg ccaccgaagc cgtccgcacc ctgatacggg cattccccct
     6001 cgtgcccgtt tcccaaccgc ccgaacaagg cagttacctc cttgctgaac acgataccgt
     6061 cagcctcagg cttgtcgggg aaaaaagcaa cgtcatcgtc gattttacct ccggcgcggc
     6121 acaataccgg cgcacaaaag gcggaggaga actcatcgcc aaagccgtca accacaccgc
     6181 gcaccccacc gtatgggatg caaccgcagg attggggcgc gacagcttcg tcctcgcctc
     6241 gctcggactg accgttaccg ccttcgagca acatcccgcc gtcgcctgcc tgctttcaga
     6301 cggcatccgc cgcgccctcc tcaatcccga aacgcaagac accgccgcgc gcatcaacct
     6361 ccatttcggc aatgccgccg aacaaatgcc tgcccttgtt aaaacacaag gcaaacccga
     6421 catcgtttat ctcgacccga tgtatcccga acgccgcaaa agtgccgccg tcaaaaaaga
     6481 aatggcttat ttccaccggc ttgtcggcga agcgcaagat gaggtcatcc tccttcatac
     6541 cgcacgccaa acggcaaaaa aacgcgtcgt cgtcaaacgc ccgcgcctcg gcgaacacct
     6601 tgccggacaa gcccccgcct accaatacac cggcaaaagc acccgcttcg acgtttacct
     6661 gccctacgga gcggacaagg gataacgccc ataaaacaag acaccgaaaa atttgccgtt
     6721 cttatgcaaa cgagaaaccg gtttttgcgt ttcgactgtt ttggataagt catcacacct
     6781 taaagtttgt cattcccacg gaagtgggaa tccgattcgt tcagttttag ttgttttcaa
     6841 gtttcaggca acttccaa
//
LOCUS       DAXDSD010000104         6705 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXDSD010000104 DAXDSD010000000
VERSION     DAXDSD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.104"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            89..493
                     /locus_tag="ACFB2Q_001815"
     CDS             89..493
                     /locus_tag="ACFB2Q_001815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC0918783.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            533..1714
                     /locus_tag="ACFB2Q_001816"
     CDS             533..1714
                     /locus_tag="ACFB2Q_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC0918784.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGTTQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(1816..4071)
                     /locus_tag="ACFB2Q_001817"
     CDS             complement(1816..4071)
                     /locus_tag="ACFB2Q_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC0918785.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     AYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNARFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAPDLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            4445..5623
                     /gene="nirK"
                     /locus_tag="ACFB2Q_001818"
     CDS             4445..5623
                     /gene="nirK"
                     /locus_tag="ACFB2Q_001818"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC0918786.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            5801..6556
                     /locus_tag="ACFB2Q_001819"
     CDS             5801..6556
                     /locus_tag="ACFB2Q_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC0918787.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGAS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
BASE COUNT         1681 a         1899 c         1685 g         1440 t
ORIGIN      
        1 tggtacaatt aacggcaatg cggacagaca aattaaacta tcagaaattg ccctaccgtt
       61 ttttaaacac tttcaggaat aaggaaaaat gaccgcccaa ccctgcccca tctgcacggc
      121 acaagatgaa gatattttgc tgcaaacccc caagctgcgc gtcatcgccg tccataacga
      181 cagcggttcg ccggcattct gccgcgtcat ttggcacgga cacattgccg aaattaccga
      241 cctttcggca gcggaacgcg gcgaattgat ggaaatggtg tacaaagtcg aagccgctat
      301 gcgccaagtg ttccggccgg caaaaatcaa cctcgccagc ttgggcaatg tcgtgccgca
      361 cctgcattgg catattatcg cccgctttga aaacgatgcg actttccctg cgccgatttg
      421 ggcaaacccc gtccgaaaac acggtatgac cctgccgcaa aattggacgg aacagcttaa
      481 aaagctgctt taaacccgcc gatgccgtct gaaaccgtat gaaagggaaa ttatgaccga
      541 accgacctcc cgccgccgtt ttctgaaaac ctgcaccgcc gccggcgcgg ggctgcttca
      601 ggcttgcggc acatccgcca catccgttcc gccccttccc tcttcccatt ccgttgtgaa
      661 agcccgaacc gtgcctctcc aaacgccacg ccgtcaaagt tcggacggca accttctgcg
      721 cgttgtcgct tcgtcaggat ttgccgaaga cactaaccgc gtcaacacag ccttaacccg
      781 cctttacaat gccggtttta ccgtaaccaa ccaacaggcg ggcagccgcc gtttccaacg
      841 gtttgccggc acggacgcgc aacgcgcggc agatttccaa gaggtcgcct ccggccgcgt
      901 cgccacgcct aaagtgctga tgggcttgcg cggcggttac ggtgcggcgc ggattctgcc
      961 gcatatcgat tttgcctcgc tcggcgcaag gatgcgcgaa cacggcacgc tgtttttcgg
     1021 attcagcgac gtatgcgccg tccaactggc attgttggca aaaggcaata tgatgagttt
     1081 tgccggcccg atggcttata gcgattttgg caaacctgcc cccggtgcgt ttacgatgga
     1141 tgcctttatc aaggggacaa cccaaaaccg cctgaccgtt gatgttcctt atatccaacg
     1201 cgccgatgtc gaaaccgaag gcacgttgtg gggcggcaac ttaagcgtcc tcgcctcgct
     1261 cgccggtacg ccttatatgc ccgacatcga cggcggcatt ttgttcctcg aagatgtcgg
     1321 cgaacagccc taccgcatcg aacgtatgct caatacgctg tatctttcgg gtattttggg
     1381 caaacagcgc gccatcgtgt tcggcgattt ccgtatggaa aaaattcgag atctctatga
     1441 ttcgtcttat gatttttccg ccgttgccaa gcatatttcg cgcacggcga aaatccccgt
     1501 gctgacgggc ttcccgttcg gacacattgc cgacaaaatc actttccctc tgggcgcgca
     1561 cacccgaatc cgtatgaacg gaaacggcgg ctattcggtc gcgtttgaag gctaccccac
     1621 actcgatgcg tccgccctga ctttggatac cctgctccca ccgccggatt tgcctatctt
     1681 ccccgaaagc ggtgttgccg atatttcgga ataaacccgc aaacggacaa atgccgtctg
     1741 aagccttcag acggcatttt cctgtaaaaa aaccgcacct gatggcaggt gcggtttcca
     1801 ttttcaggct aaaacttatt tgtcgcggcc gaatacgatt ttagtggctt ggatggcaac
     1861 gcagattgca ccgccgataa agattaagtc gggggcagtg cgtacccagc gcagggtgtc
     1921 gaggatttcc atttgcagga attcttcgct gcgggcatac cacaaaccgt gcgtgataga
     1981 ggcgtatgcc tgaatcacgc cgacaggcag caggctgatg gcaatcatac cgaccaagcc
     2041 gccgttgagc agccagaagc cccaagtcat cagtttgtcg tcaaaccgcg cgttcggttt
     2101 caagtagcgc gcaaccaaca atacgaagcc caatgccaag aaaccgtaca caccgaacaa
     2161 ggcggcgtgc gcgtgaacgg cggaagtgtt caaaccttgg atatagaaca gggaaatcgg
     2221 cggattgatc agaaagccga atacgccggc accgatcata ttccaaaaag cgactgccac
     2281 gaagcacatc agcggccaac gcaggcgttt cgcccagtcg gacaggtgtt ggtaagacca
     2341 gtgctcgtat gcctcgcggc ccagcaacac cagcggcacg acttccaaag cggagaagca
     2401 ggcgccgatt gccatagagg cggaggtaga gccggagaag tacaggtggt gcagcgtgcc
     2461 cggaacgccg cccaacataa agatggcggc agcggccaaa gtggaggcag tggcggtact
     2521 gcggcggaca aagcccatat tgtagaagac aaaggcaaag gcggcagtgg caaatacttc
     2581 aaagaagcct tccacccaca ggtggaccac ccaccaacgc cagtattcca ttacggcaat
     2641 cggggatttt tcgccataga acaggcccgg tgcgtagaac acgcccacgc cgaccataga
     2701 agcgacaaag attgccagca ggtttttgtc cacgcctttt tctttgaagg cggaaaccgt
     2761 gcagcgcaac atcaggaaca gccacaacag cagtccgacc atcaaaagga gttgccagaa
     2821 acgtcccaaa tcgaggtatt cgtaaccttg gtgtccgaac cagaagttaa attcgggggg
     2881 aaggatgtgc gtcaacgcga agaagttgcc cgcgtaagaa ccgccgacca cgatgaagag
     2941 ggcgatatag aggaagttca cgcctgcacg ttggaacttg ggatctttgc cgccgttgac
     3001 aatcggcgcg aggaacaaac ctgccgtcaa aaagccggtt gcaatccaga agatggcgga
     3061 ttggatgtgc caagtacggg tcagggcgta ggggaaccag tcggacattt caaagcccaa
     3121 cgcctcgtca atgccgtaga aaccctgacc ttcgacggtg tagtgcgcgg tcaggccgcc
     3181 cagcaatact tgtaccacaa acagggcgac cgtcaggaag acgtatttgc ccaatgcttt
     3241 ttgcgaaggg gtcagttgga ttttggaaat cgggtcttca gacggcactt ccacttcctc
     3301 gtgtttggtc aggaaggaat aaccccacat caacaaaccg atgcccatca agagcagtac
     3361 gacactggta aacgaccaca tgtagttttc agtagtcggt acattgttga tcaaaggctc
     3421 gtgcggccag ttgttggtgt aggtaaagac ctcgccggga cggttggtcg aagcagacca
     3481 agaagtccag aagaagaagt cgaacagttt ttcacgcgct tcttggcttg gcaatgtgtt
     3541 gtttttcatt gcaaagtgtt cgcgggtggt ttgcaacttg ggatcatcgc cgtaaacgcc
     3601 gtgatagtaa ggcaggatgc tttcgatggc tttcacgcgc gtatcgctga tgacgacgct
     3661 gccgtcttct ttaatacggc tttgattgcg gtattcatcg gccaagcggg ttttcagaac
     3721 ggcttgttct tcaggggaaa cttcatcgaa ttttttgccg taagcctgtt gcgcggtcaa
     3781 atccaaccag gcggacaact cacgatgcag ccagtcggcc gtccaatccg gagcctgata
     3841 tgcgccgtgt cccagaatcg aaccgacttc catgccgccg gtagtctgcc acgcagactg
     3901 acctgccaaa atatcgtctt tcgtcatcag cactttgcct gatgcggaaa cgacctgttc
     3961 ggggtaaggc ggggctttct tataaacctc gctgcccata tagccaagaa tggtaaagca
     4021 taccgccaga acggcaaaca gcaagtacca cagcttcttg tactgtccca ttttgagagc
     4081 tccttttaat aaatcagttg tttaaaattc acaaaatatg aatgttaaag attgtagcat
     4141 ggtttaccgc gcaaataaac atttgttcaa acaaactcac atatagaaca aatacatata
     4201 tgataataac tatcattatt ctttactcgg aaattgccct gcctttgcct gctctgccgg
     4261 agcccctagc aaatcagcct attcattgta atttttagta gttataaagt attagaagcg
     4321 tcattttaag ttcatatttt atgaattatt tgacttaaat taaaatgccc gcaatgggac
     4381 aaccgcataa tcacaccaag ttcttaacta atccccccta cttttcttac aaaaggaaaa
     4441 cattatgaaa cgccaagcat tagccgcaat gattgcttcc ttattcgcat tggccgcctg
     4501 cggtggcgaa cctgccgcgc aagcccctgc cgaaacccct gccgcttccg cagaagccgc
     4561 aagttccgcc gcacaagcta ccgccgaaac gcctgcaggc gaactgcccg tcatcgatgc
     4621 ggtgaccacc cacgctcccg aagtacctcc cgcaatcgac cgcgactatc ctgccaaagt
     4681 acgcgtaaaa atggaaaccg tcgaaaaaac catgaaaatg gacgacgggg tggaataccg
     4741 ctactggaca tttgacggcg acgttccggg ccgtatgatc cgcgtacgcg aaggcgatac
     4801 ggttgaagtc gaattctcca acaatccttc ttctaccgtt ccgcacaacg tcgacttcca
     4861 cgccgcaacc ggtcagggcg gcggtgcagc cgcgaccttt accgccccgg gccgcacttc
     4921 cacattcagc ttcaaagccc tgcaaccggg cctgtacatc taccactgcg ccgtcgcgcc
     4981 ggtcggtatg cacatcgcca acggtatgta cggtctgatt ttggtcgagc ctaaagaagg
     5041 cctgccgaaa gtggataaag agttctacat cgtccaaggc gacttctaca ccaaaggcaa
     5101 aaaaggcgcg caaggcctgc aaccgttcga tatggacaaa gccgttgccg aacagcctga
     5161 atacgtcgta ttcaacggcc acgtaggcgc tatcgccggc gataacgccc tgaaagccaa
     5221 agcaggcgaa accgtgcgta tgtacgtcgg taacggcggc ccgaacttgg tgtcttcctt
     5281 ccacgtcatc ggcgaaatct tcgacaaagt ttatgttgaa ggcggcaaac tgattaacga
     5341 aaacgtacaa agcaccatcg tgcctgccgg cggttctgcc atcgtcgaat tcaaagtcga
     5401 catcccgggc agctacactt tggtcgacca ctccatcttc cgcgcattca acaaaggcgc
     5461 gttggggcaa ttgaaagtag agggtgcgga aaaccctgaa atcatgactc aaaaattgag
     5521 tgataccgct tacgccggca gcggcgcggc ttctgcccct gctgcttccg caccggctgc
     5581 ttctgccccg gcagcctctg catccgaaaa aagcgtttat taaatcggat acccgtcatt
     5641 agcgggacaa accactgccg ccgtacttca ttacgcacgg cagtggtttt ttaacaacca
     5701 atctttcctt tcggaagatt gatttttcag acggcattcg cggctttttg tttaaaatgc
     5761 cgtctgaaaa atcaatgcta accgcctgtc aggaggcttt atgaagtacg tccggttatt
     5821 tttcctcggc acggcactcg ccggcactca agcggcggct gccgaaatgg ttcaaatcga
     5881 aggcggcagc taccgcccgc tttatctgaa aaaagatacc ggcctgatta aagtcaaacc
     5941 gttcaaactg gataaatatc ccgttaccaa tgccgagttt gccgaatttg tcaacagcca
     6001 cccccaatgg caaaaaggca ggatcggttc caaacaggca gaacccgctt acctgaagca
     6061 ttggatgaaa aacggcagcc gcagctatgc gccgaaggcg ggcgaattaa aacaaccggt
     6121 aaccaatgtt tcctggtttg ccgccaacgc ctattgcgcc gcacaaggca aacgcctgcc
     6181 gaccatcgac gaatgggaat ttgccggact tgcttccgcc acgcagaaaa acggctcaaa
     6241 cgaacccggc tacaaccgca ctattctcga ttggtatgcc gacggcggac ggaaaggcct
     6301 gcacgatgtc ggcaaaggcc gcccgaacta ctggggtgtt tacgatatgc acgggctgat
     6361 ttgggaatgg acggaagatt tcaacagcag cctgctttct tccggcaatg ccaacgcgca
     6421 aatgttttgc agcggcgcat ctgtcggggc gagcgactcg tccaactatg ccgccttcct
     6481 ccgctacggc atccgcacca gcctgcaatc caaatatgtc ttgcacaact tgggcttccg
     6541 ctgcgcaagc cgataacccc ttcaattata gtggattaac aaaaaccagt acggcgttgc
     6601 ctcgccttgc cgtactggtt tttgttaatc cgctatattc cgccatctct aagatttaca
     6661 gcgatacacg ggtaatttaa ggaatgcccg aaccgtcatt cccgc
//
LOCUS       DAXDSD010000105         6594 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXDSD010000105 DAXDSD010000000
VERSION     DAXDSD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.105"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..262)
                     /locus_tag="ACFB2Q_001820"
     CDS             complement(<1..262)
                     /locus_tag="ACFB2Q_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918788.1"
                     /translation="MPSLQRRSRGEKSSCIKQLNGGNYPPFLICCQLFFENLPDRFKF
                     CKKLARWLSGNKFPQKPVNPIIQKIICFHRVSFSVETLPFRAV"
     gene            complement(571..1035)
                     /locus_tag="ACFB2Q_001821"
     CDS             complement(571..1035)
                     /locus_tag="ACFB2Q_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC0918789.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHAHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            1257..2315
                     /gene="alr"
                     /locus_tag="ACFB2Q_001822"
     CDS             1257..2315
                     /gene="alr"
                     /locus_tag="ACFB2Q_001822"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC0918790.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(2857..4212)
                     /locus_tag="ACFB2Q_001823"
     CDS             complement(2857..4212)
                     /locus_tag="ACFB2Q_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC0918791.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(4227..4499)
                     /locus_tag="ACFB2Q_001824"
     CDS             complement(4227..4499)
                     /locus_tag="ACFB2Q_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC0918792.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(4624..5361)
                     /locus_tag="ACFB2Q_001825"
     CDS             complement(4624..5361)
                     /locus_tag="ACFB2Q_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC0918793.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="ACFB2Q_001826"
     CDS             complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="ACFB2Q_001826"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC0918794.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
BASE COUNT         1756 a         1640 c         1470 g         1728 t
ORIGIN      
        1 ctaccgccct aaagggcagg gtttcaaccg aaaaggaaac acgatgaaag catatgattt
       61 tttgaataat cggattgacg ggtttttgag ggaatttgtt accggatagc catcgggcaa
      121 gttttttgca aaatttgaat cggtcgggta aattttcaaa aaataattga cagcagataa
      181 gaaacggcgg ataattaccg ccgttgagtt gcttgatgca gcttgatttt tctcctctac
      241 ttctcctttg tagacttggc acacattcaa ccggatgtgt gcatttttta tctccgcttt
      301 ttttgaaatt taattacttt tattgtttga aattctattc tttaagaaat ttaacaagaa
      361 aaacgcttgc tttttgaccg gaaaagctgc ataattcgtt ccgttagctg gttcgagtag
      421 tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt ctattgaatg
      481 tgtgcatttt ttatctgaag caacaagcct ctgtgcgtga tgttgttatg tttcatttag
      541 atgtcaaacc gcatacccgg tctgaaatat tcaatccaaa tccaaaaccg gattttcttt
      601 gacttcctcc atcacaacat aactcctact ctccgaagcg gcaggcagtt gcaataggat
      661 attgccgagc atatcccgat aggcagacat atcgggcaaa cgtactttaa tcaggtagtc
      721 gtattcgccc gacaccagat ggcactccat aatttgcgga attttcagca cttctttttt
      781 gaaatcttcg aaaatattgc ccgatttgga ttgcagcttc agctcgacaa aaaccaatgg
      841 cggttttccc aacagatggg gattgagatg ggcgtgatag ccggaaatat aatgttcccg
      901 ctccaaacgg cataccctct ctgtaacggg cgtgctggac aaccctacct tctcggcaag
      961 ctccgtcatc gggatacggg cattttgctg aagaattttg aggatgcgga aatcgatttt
     1021 atctagttct ttcatttgga ttaccttgta tttattattg attttaacaa atagagtata
     1081 aatttgggaa agcaattata tcaggaaaag caaaccgcct tcctacctga aaactgctgc
     1141 tctggcttga agacacaagg ttctttaata ttttaaaagc cttgccgttg gattataatt
     1201 ccccaattga tttcttaatt ttgctaataa acacttgttt ggtaaggaat gaatttatgc
     1261 ggcctttgaa cgtgcagatc aggttgggca accttaggca caattatcgg attttgaagg
     1321 aaatgcacgg aggcaagctg ttggcggtag tgaaggccga cgcatacgga cacggtgcgg
     1381 tcagatgtgc tttcgcgctg gcagacttgg cagacggctt tgccgtggcg acaatcgacg
     1441 agggaatcag gctgcgggag agcggcatta cccatccgat tgtcctttta gaaggcgtat
     1501 ttgaagcatc agagtacgaa gcggtcgaac aatactcgct ttggccggca gtcggaaacc
     1561 aatggcagct tgaggctttg ctttcccgcc attggaaaaa acctgtcaag gtctggttga
     1621 aaatggattc gggaatgcac cgtaccggat ttttccctca tgattacgct tcggcatatg
     1681 cggcattgaa acagtcggaa tatgtggaca gtattgtcaa gttctcgcat ttctcctgtg
     1741 cggacgaacc tgaaagcggt atgacggaaa tacagatgga agcattcgat ttgggtacga
     1801 aagggctgga aggcgaagaa agccttgcca attcggcagc tattttgaat atccccgaag
     1861 cacgcaggga ttgggggcgc gcgggcttgg cgttatacgg catttccccg ttcggaggca
     1921 gcgatgacag gctgaaaccc gtgatgaggc tttcaacccg tattttcggc gaacgcgttt
     1981 tacagccgca ctcccctatc ggttatggcg caacatttta taccagtaaa tctacgcgcg
     2041 tcggtctgat tgcctgcggt tatgcggacg gttatccgcg ccgcgctcca agcaattccc
     2101 ccgtcgccgt cgacggtaaa ttgacccggg tcatcggcag gatctctatg gatatgatga
     2161 ctatcgagct ggacgcttcg caagaaggtt tgggacacga ggtcgaactg tggggcgata
     2221 cggtcaatat caataccgtt gccgaagcgg ccggaaccat cccttacgaa ttgatgtgca
     2281 atatcaaacg tgcaaaattc acttatatcg agtaatcgag tccaaacgaa aatcccgtct
     2341 gaagcttttc agacggcata aatgcgtttt gacaaaatca gtaaaagaca ttgccaaata
     2401 gaaaatactg ataatgccca caatctgaaa ccccatcctt cccgcaaaaa cggaaaatca
     2461 aaatcagaaa cctaaaattt cgtcattccc gcgcaggcgg gaatccggtt ttttgagttt
     2521 ccgtcatttc tgataaattc tcgtcgtttt tcatttctag attcccgcat gcgcgggaat
     2581 gacaaaatta aagtttcaga atttatttga acaacaaact agattcccgc tttcgtgggg
     2641 atgacggcgg ataggttttg ttgtttcgga taaattcctg ttgttttttg gtttccagat
     2701 tcccaccttt aaaagaatga cgtaagaaag cagaaataag aacacgggga ttttttaaat
     2761 ttgcaatatt cttttctaaa gacattttac ccaatgccca taaacaaaaa tgccgcctga
     2821 agcctttcag acggcatttt cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg
     2881 tgcgggaatc ctgccgcccc ggttgacgaa cacttcgcac gaaccttctt ttgccggcat
     2941 tacaggcgcg tagcccaaca gaccgccgaa ctcgacgctg tcgccgacgg ttttgcccgt
     3001 taccggaata atgcgcacgg cggtggtttt gctgttgatc atgccgatgg cggcttcgtc
     3061 ggcgatgatg ccggaaatgg tgtgcgcggg cgtgtcgccg ggaacggcaa tcatgtccaa
     3121 accaacggag cagacggcgg tcatggcttc gagtttgtcc agcgtcaaca cgcctgcctc
     3181 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccgc tcaaaccgcc
     3241 gaccgcgctg gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     3301 tgctgttgtg ccgtgcgtac cgcagacgct caagcccatt tcttcaagaa tgcgcgccac
     3361 cgagtcgccg acggcggggg tcggtgccag cgacaaatcg agaatgccga acgggatatt
     3421 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtgattt tgaaagcggt
     3481 tttcttcacg acttcggcga cctcggtcag gctgaccgcg tccgaatttt ccagcgcggc
     3541 tttgaccacg cctggaccgg atacgccgac attaatcaca gcatccgctt cgcccgagcc
     3601 gtggaacgca cccgccataa acggattgtc ttccaccgcg ttgcagaaca cgacgatttt
     3661 ggcgcagccg aaaccttcgg gcgtgatttc agccgtgcgt ttgatggttt cgcctgccag
     3721 cttgaccgca tccatattga taccggcacg cgtgctgccg atattgatgg agctgcacac
     3781 gatatcggta gttttcatcg cttcgggaac ggaacggatc aacacctcat ccgaaggcga
     3841 catacctttt tgcaccagcg cggaaaagcc gccgataaag gacacgccga tggctttggc
     3901 tgccttgtcc aaagtctgcg ccacgctgac ataactgtcg gctttggtcg ccgccgcgat
     3961 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcgga
     4021 aaggtgtttc gccgttgcca ccaagtcttt gccgaccgtg gtgattttgt tgtaaatgtt
     4081 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     4141 ggtgcggaca tcaaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     4201 ttggatactc atcacattcc tccgactcaa atgcggtgca tcgcttggaa gatttcttcg
     4261 ttttgcatac ggatgtcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaatc
     4321 tcctgccgcg atttagggca tttcgaggta tccaccaaga taatcatagt aaaaaaatcg
     4381 tccatcagct gttggctgat attgaggatg ttgattcggt tttccgccaa aattttggaa
     4441 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     4501 ggcttactcc ttgcagatat ccgttaaaat ccgaaattat accaccgtcg gattttgaag
     4561 aatattgtca acaatatata catacaaaat gccgtctgaa actatttcag acagcatcaa
     4621 gattcagggt tcgattaaat aaccatcctt atcccattgg gttttcctga ccaacttgtc
     4681 atcctgataa acagcctcgc tctttttaga accatcttca taccactcca aaaccacccc
     4741 gttgcgttta tggtggcgga tagacagttc cgaaagtaat cggccgcttt catcccaagt
     4801 cagaattttg gcaggctcat cgttgaccat aaccatttcc gtcttgatac ttccatcggc
     4861 ataccattgc ttccatacgc cgttcgcctt attttgctta aactggattt cgctttcctt
     4921 gccgccgtta cggtaataac ggtatcccgt accctcactc aagccatttt tataaggcat
     4981 aaccgcagat tttttaccgt tcggatacca gttgacccat tccccgtccg gcttaccctt
     5041 gctgaagccc cccgccattt ttttctgacc attaaaatgc cacaaaatca acataccgtt
     5101 ttgcagggta ggcacaaaag atttgatttg cgttgaagca acgatataag gttcggaata
     5161 tttcttcatc gacggataat aaaaatcctg cgcgtgtgcg atacccgccg ccacactata
     5221 ttgcctgata taagcggcag aagacatcgt cgccgtcagt ttcccgttct gattaaaata
     5281 aacagaatag gtctgcgccg gcaaagcggc cgaaaaaccc aacagtacga ttgaaaatac
     5341 aatccgagat aatttcttca ttatgatagc gatataaaaa acaattaact tataaacaat
     5401 atggtaagga tttctcaacc aagcgtcaaa cccgaaacaa tgtatcgtaa aaatgccgtc
     5461 tgaaaacaaa tcgtcttcag acggcatttc cctccaactc actcttcgcc caataactgc
     5521 tcgcgcgtca agaggaaaac aaaaccgtcg cccccgctgg tttccaacca agtaaaaggc
     5581 aactccggat acgctgcttc caatacatcc ctgttatgcc cgatttccac cagcaataca
     5641 cctttaggat tcagaaactt tgccgcattc agaagaatct gcctggtggc atccaacccg
     5701 tccgccccgc tgcccaacgc caattccggt tcgtgcaaat attcctcagg caaagcccca
     5761 accgactctg catccacata aggcggattg gaaacaatca aatcataagt gccttccagt
     5821 ccctcaaaca aatcggtatg aataagctga atacgctctt ccagaccata atcttcaata
     5881 ttgatccctg caacttccaa agcatccaaa ctcacatcaa ccgcatcaat ttgggcatca
     5941 ggataatggt gcgccatctg aatggcaagg caaccgcttc cggtgcaaag atccaaagca
     6001 ttatgcacca gctcatcgta ttctatccaa ggtcgaagtc cgtcacccag caattcataa
     6061 ataaaagagc gcggtacaat cacgcgttca tctacataaa aatcaaactc cccctgccag
     6121 gcctggtgtg tcaaataagc ggcaggaatg tgttcgacag cacgacgctc aataaccgcc
     6181 agcacttcct ctttttcagc ttccaagagt tttgcatcaa gatatggggc aagcatatcc
     6241 aaaggcaaat tcaaagtatg cagaatcaaa taaaccgctt catcatgcgc attatctgtt
     6301 ccatgaccaa aaaagagccc tgcctcatta aaacggctga ctgcaaaacg taaaacatca
     6361 cggatagtcg tcaattcttg tgcggcctga ttaaacataa tatgaaccat tctgcgtata
     6421 gatgctttta attataacag aaacaacaag caaacctttt catatcgcca aataaccact
     6481 caatctaccc atacaactac ataaatgccg cgcgaagacc attgcccgaa cggaaacggc
     6541 gatggcggac ggtatgggca acctgatagg ctgggaagaa acggggcttg ttga
//
LOCUS       DAXDSD010000106         6488 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXDSD010000106 DAXDSD010000000
VERSION     DAXDSD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.106"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1356
                     /gene="carB"
                     /locus_tag="ACFB2Q_001827"
     CDS             <1..1356
                     /gene="carB"
                     /locus_tag="ACFB2Q_001827"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC0918795.1"
                     /translation="LEIVRTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSID
                     AAEDRERFQKVLNDLGLRQPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIV
                     HSAEALQKYMREAVQVSEDSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAG
                     IHSGDSGCSLPPYSLSEEIQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNP
                     RASRTVPFVSKATGVPLAKVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPG
                     VDTILSPEMRSTGEVMGVGASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKT
                     AKNFQALGYGICATRGTAQYLTEHGLIVQAINKVPEGRPHIGDALKNGEIALVVNTVS
                     SDPQSVSDSHIIRQSALQQRVPQYTTTAGGEAMSEGAKSRDYLGVYSVQELHGRLKNR
                     N"
     gene            complement(1557..2597)
                     /gene="queA"
                     /locus_tag="ACFB2Q_001828"
     CDS             complement(1557..2597)
                     /gene="queA"
                     /locus_tag="ACFB2Q_001828"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC0918796.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            2714..2971
                     /locus_tag="ACFB2Q_001829"
     CDS             2714..2971
                     /locus_tag="ACFB2Q_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC0918797.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            2953..3414
                     /gene="accB"
                     /locus_tag="ACFB2Q_001830"
     CDS             2953..3414
                     /gene="accB"
                     /locus_tag="ACFB2Q_001830"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC0918798.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            3528..4889
                     /gene="accC"
                     /locus_tag="ACFB2Q_001831"
     CDS             3528..4889
                     /gene="accC"
                     /locus_tag="ACFB2Q_001831"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC0918799.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTFADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIPDKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            4993..5880
                     /gene="prmA"
                     /locus_tag="ACFB2Q_001832"
     CDS             4993..5880
                     /gene="prmA"
                     /locus_tag="ACFB2Q_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC0918800.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLSGVKR"
     gene            5898..6461
                     /gene="orn"
                     /locus_tag="ACFB2Q_001833"
     CDS             5898..6461
                     /gene="orn"
                     /locus_tag="ACFB2Q_001833"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC0918801.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
BASE COUNT         1568 a         2033 c         1679 g         1208 t
ORIGIN      
        1 ttggaaatcg tccgcaccga aaacccgtgg ggcgtgattg ttcattacgg cggtcaaacc
       61 ccgctgaaac tcgccaacgc attggttgaa aacggcgtga acatcatcgg cacatccgcc
      121 gacagcatcg atgccgccga agaccgcgaa cgcttccaaa aagtgttgaa cgacttaggc
      181 ctgcgccaac cgcccaaccg catcgcccac aacgaagaag aagcactcgt caaagccgaa
      241 gaaatcggtt atccgctggt cgtgcgcccg tcttatgttt tgggcggacg cgcgatgcag
      301 attgtccact ccgccgaagc cttgcaaaaa tacatgcgcg aagccgtgca ggtttccgaa
      361 gacagccccg tgctgctcga tttcttcctc aacaacgcga ttgaagtcga tgtggactgc
      421 gtttcagacg gcaaagatgt ggttatcggc ggcatcatgc agcacgtcga acaggcgggc
      481 atccactccg gcgactccgg ctgctcgctg ccgccatact ccttaagcga agaaatccaa
      541 gacgaaatcc gccgccaaac caaagcgatg gcgtacgcgc tgggcgtggt cggactgatg
      601 aacgtgcagt ttgccgtgca ggacggcgtg gtgttcgtgt tggaagtgaa cccgcgcgcc
      661 agccgtaccg tcccctttgt ctccaaagcc accggcgtgc cgctcgccaa agtcggcgcg
      721 cgctgcatgg caggcatttc cctgaaagaa caaggcgtgg aaaaagaagt cgtccccgat
      781 ttctatgccg ttaaagaagc cgtgttccca ttcatcaaat tcccgggcgt ggatacgatt
      841 ttgagtccgg aaatgcgctc caccggcgaa gtgatgggcg tgggcgcaag tttcggcgaa
      901 gcctactaca aagcccaact cggcgcgggc gaacgcctca acccgaccgg caaaatcttc
      961 ctctccgtgc gcgaagaaga caaagaacgc gtcattaaaa ccgccaaaaa cttccaagcc
     1021 ttaggctacg gcatctgcgc cacgcgcggc acggcgcaat acctgaccga acacgggctg
     1081 attgtgcagg ccatcaacaa agtccccgaa ggccgcccgc acatcggcga cgcgctgaaa
     1141 aacggcgaaa tcgcactggt cgtaaatacc gtttccagcg atccgcaatc cgtgtccgac
     1201 agccacatca tccgccaaag cgcattgcag caacgcgtgc cgcaatacac caccaccgcc
     1261 ggcggcgaag cgatgagcga aggcgcgaaa agccgggatt atctgggtgt gtacagcgtt
     1321 caagagctgc acggtcgttt gaaaaaccgc aactgatgcc tgaatcaggt tgaacaatgc
     1381 cgtctgaagc cgttttgcgg tttcagacgg cattttgttg cacatagcct tcactttctt
     1441 ttgaccttta agcaccctaa tttggggcgg tggattatcc aactgccgga catccataaa
     1501 aataccgccc gaaccacggt tcagacggca tctgccttgc aacggacagc agcagtttaa
     1561 agcccgcccc cttcgttccg ccccaaaacc atcgcatctc cgtagctgaa gaaacggtat
     1621 tcacgttcaa tcgcatgacg gtacacggcg cggatatgac ccatacccga aaacgcgccg
     1681 accaacatca gcagcgtcga tttcggcaga tgaaaattgg taaccagcct gtcgacaaca
     1741 ttaaaacggt agcccggcgt gatgaaaata tcggtgtcgc cctgtccgtc tttcaaatat
     1801 cccgttgcgc gcgcggcaga ctcgagggcg cgcatggaag tcgtgccgac cgcccaggct
     1861 ttgttccccc gggcttttgc cgcctcaacg gcggcgacgg tttcagacgg cacttcaaac
     1921 cattcgctgt gcattttgtg ttcttcgatt ttatcgacgc gcacgggttg gaatgtcccc
     1981 gcaccgacgt gcagggttac ttctgcggtt accgcgcctt tgtctttcag acggcgcaaa
     2041 agttcttccg taaaatgcag gcccgccgtc ggcgcggcga ccgcgccctg atatttggca
     2101 taaacagttt gataacggct gtcgtcgtcc gcatcggcgg cacgttcgat ataaggcggc
     2161 aggggcaggt gtccgttctg ttccaaaagt tcgtaaacgg tttgaccgcc ttcaaaacgc
     2221 aggcagaaca gttcgccctc acgcccgacc atcacggcac ggataccgcc ttcaaacacc
     2281 agccccatac cgggcttggg ggacttggac gaacggatgt gcgccaatgc ggtatggttg
     2341 tccaacacac gctcaatcag ggcttcgatc ctgccgccgc tgtctttctg cccaaacagc
     2401 cgcgccttca tgactttggt gttgttgaat accaaaacgt cgcctgcctc gacataatcg
     2461 ggcaaatcgc caaacacccg gtcttgcagc ggcatatcgg gcagcgcgac caaaagccgg
     2521 ctgctgccgc gcacctcggg cggatgctgg gcaatcaggt gttcgggcag ggtaaagtca
     2581 aaatctgaaa tatccatttt tacactctcg ttcgggcaag ccgccattat acgcacttta
     2641 gccctttttc agacggcatc tttgcccgaa aaaccaacag attagaataa acactcttga
     2701 ctcggggcat cttgtgcgca aaatcaaact tcctgcacat ttcccccaaa aaccgccgtt
     2761 ttttgatatt ttactggaca tttaccgaca acttcgggaa aatgaacaca atttcacggt
     2821 cgtttcccac cacaggaaaa ccgtatccga acaccattcc gcccggtttg cgccgttgcc
     2881 gcaagccggc tgttttctga aaaaccaacg caacaacccg ccggaacacc ggcagccttt
     2941 aaaggaacag aaatggattt gcgcaaatta aaaaaactga ttgatttggt tgaagaatcg
     3001 ggtatcgccg aaatcgaagt aaccgaaggc gaggaaaaag tccgcattac ccgtaccatt
     3061 gcagctgcgg cagctcccgt ttacgccgcg cccgtacctg ccgccgcgcc ggccgtaacg
     3121 ccggccgccg cacccgttgc ggcatccgcg cccgccgccg cgccggccgc ccgcgatttg
     3181 tccgacgctc aaaaatcgcc tatggtcggc acgttctacc gcgcgcccgg cccgaatgcc
     3241 gccgcatttg tcgaagtcgg tcaacaggtc aaagccggcg acacgctgtg catcatcgaa
     3301 gcgatgaagc tgatgaacga aatcgaagcc gaaaaatccg gcacggtcaa agaaattctg
     3361 gtcgaaaacg gtacgcccgt cgaattcggc gaaccgctct tcatcatcgg ataatcctgt
     3421 tttcagacgg cataaacttc cgatgccgtc tgaaatgctt tccccccctt cagcgttccc
     3481 gcaccctttt ttacggacgg gttgccggaa ccgcaggaaa ggtcatcatg ctgaaaaaag
     3541 ttttaatcgc caaccggggc gaaatcgcct tgcgcgtact ccgtgcctgc cgcgaaatgg
     3601 gcattgccac cgttgccgtg cattccgaag ccgacaaagg cagcctgcac gtcaaactcg
     3661 ccgacgaatc cgtgtgcatc ggtcctgccg cttctgcgca aagttatctg aacattcccg
     3721 ccatcattgc cgccgccgaa gtaaccttcg cggacgctgt ccacccgggc tacggcttcc
     3781 ttgccgaaaa cgccgatttc gccgaacagg tcgaacagtc cggctttacc ttcatcggcc
     3841 cgaaacccga caccatccgc ctgatgggcg acaaagtctc cgccaaacac gcgatgatag
     3901 cggcaggcgt gccctgcgtc cccggctctg acggcgcatt gcccgacgat gatgccgaaa
     3961 tcctcaaaat ccccgacaaa gtcggttatc ccgtgattat caaagcatcg ggcggcggcg
     4021 gcgggcgcgg tatgcgcgtg gtcgagaaaa aagaagacct cctccagtct gtcgaaatga
     4081 ccaaggctga agcgggcgcg gcgttcggca acccgatggt ttatatggaa cgctacctgc
     4141 aacgcccgcg ccacgtcgaa atccaagtgc ttgccgacga acacggcaac gccgtctatc
     4201 ttgccgagcg cgactgttcg ctgcaacgcc gccaccagaa agtcatcgag gaagcacccg
     4261 ccccgtttat cgatgaagag gcacgcaaaa aaatcggcaa agcctgtacc gacgcgtgca
     4321 aacgcatcgg ctaccggggc gcgggcacgt ttgaattttt atacgaagac ggcgaatttt
     4381 tctttatcga gatgaacacg cgcgttcagg tcgagcatcc ggttaccgaa ctcattaccg
     4441 gcgtggacat cgtccaagag caactccgca tcgcatccgg cctgccgctg caatacaaac
     4501 agaaagacat caaaatcgaa ggccacgcct tcgagtgccg catcaatgcc gaagacccgt
     4561 acaacttcat ccccagcccg ggcccgattg aaagctgcca cctgcccggc ggcttcggca
     4621 tccgcgtgga cagccacatc taccaaggct accgcatccc gccgtactac gacagcctga
     4681 tcggcaaaat ctgcgtacac ggcaaaacgc gtgaacaggc aatggcgaaa atgcgcgtcg
     4741 cactcgccga gctggcggta accggcatca aaaccaatac gccgttgcac cgcgacctgt
     4801 tcgccgatgc gggtttccaa gaaggcggag tgagcatcca ttacctggag cactggctgg
     4861 aagcgcgcaa aaccaaacag gacaaataag ccgccgccga tatgccgtct gaagccgccc
     4921 ttccgcgttc agacggcatt tcccttgccc cgccgtctga aaccgatttc gatatttcca
     4981 agaaagcccg ttatgcccta ccaacaaatc accgtcaacg tcaacgatgc cgtcgccgaa
     5041 cgcctcgccg acgcgctgat ggaacacggt gcactctccg ccgccatcga agatgcctgc
     5101 gccggcacgc aaaacgaaca ggcgattttc ggcgaacccg gtatgcccac cgaacaaatc
     5161 tggcagcaga gcaaagtcat cgccctgttc ggcgaacacg acgaagccgc cgccgtcatc
     5221 gatgccgccg cacaagaatg cgggttaaaa gacttggcat acaccggcga aaccatcgaa
     5281 gaccaagact gggtgcgcct cacgcaatcg caattcgacc ccatccgaat ttccgaccgc
     5341 ctgtggatta ccccctcttg gcacgaagcc cccgaaggct gcgccgtcaa cctccgcctc
     5401 gatcccggac tcgccttcgg caccggcagc cacccgacca cgcgcctctg cctcaaatgg
     5461 ctggatacgc aactcaaaaa cggcgaaagc gtcctcgact acggctgcgg ttcgggcatc
     5521 ctgaccatcg ccgccctcaa actcggtgca ggttccgccg tcggcgtgga tattgacgaa
     5581 caggccgtcc gcgccggcag ggacaacgcc gaacagaaca acgtcgatgc acaattcttc
     5641 ctgcccgaca gtctgcctca agggcaattc gatgtcgtcg tcgccaacat cctcgccaac
     5701 cctttgcgta tgctcggcga aatgcttgca gcccgcacca aacagggcgg acgcatcgtg
     5761 ttgtccggtt tgttggatga acaggccgaa gaactcggcg gcatttacag ccaatggttc
     5821 gatctcgacc cggcggaaac tgacgaaggc tgggcgcgct tgagcggcgt aaaacgctga
     5881 aacggaaagg aaacaccgtg caggacaaaa acaacctctg ctggctcgat atggaaatga
     5941 cggggctgaa tcccgaaacc gaccgcatta tcgaagtcgc gatgattatt accgactcgg
     6001 atttgaatgt gttggcgcaa tccgaagttt acgccatcca ccaaagcgac gatctgctcg
     6061 acaatatgga cgagtggaac accgccacac acggcaggac ggggctgacg cagcgcgtgc
     6121 gcgaatcgtc gcataccgaa gccgaagtcg aacagaaact gctggacttt atgtcggaat
     6181 ggatacccgg acgcgccaca ccgatgtgcg gcaactccat ccaccaagac cggcgtttta
     6241 tggtcaaata tatgccgaaa ctggaaaact acttccacta ccgcaacctc gacgtttcca
     6301 cgctgaaaga actcgccaaa cgctggaatc cgcccattgc caaaagcgtc gtcaaacgcg
     6361 gttcgcacaa ggcattggac gacattttgg agagcatcga agaaatgcgc cactaccgcg
     6421 aacacttcct gatttccgcc ccgaaagccg aagcgcaata agaaacaaac aatgccgtct
     6481 gaaacgct
//
LOCUS       DAXDSD010000107         6363 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXDSD010000107 DAXDSD010000000
VERSION     DAXDSD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.107"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..398)
                     /locus_tag="ACFB2Q_001834"
     CDS             complement(<1..398)
                     /locus_tag="ACFB2Q_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918802.1"
                     /translation="MVIFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSR
                     PEFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAG
                     IRVILPIYLQQAQQDKMLALYAQGILAQADG"
     gene            complement(640..897)
                     /locus_tag="ACFB2Q_001835"
     CDS             complement(640..897)
                     /locus_tag="ACFB2Q_001835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC0918803.1"
                     /translation="MRAKTRFALRNGVNLQPFAAFNVLHRSKSFGMEMDGEKQTLAGR
                     TALEGRFGIEAGWKGHMSARIGYGKRTDGDKEAALSVKWLF"
     gene            823..984
                     /locus_tag="ACFB2Q_001836"
     CDS             823..984
                     /locus_tag="ACFB2Q_001836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918804.1"
                     /translation="MQNIKSGKRLKIDTVTQSKTGFCPNAGADLPLTAAEQPDRRPSL
                     SVKPPFTPK"
     gene            complement(1081..1494)
                     /locus_tag="ACFB2Q_001837"
     CDS             complement(1081..1494)
                     /locus_tag="ACFB2Q_001837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918805.1"
                     /translation="MSFDCFGAFSYQPGSDTRPTNVGDTADFSFSDKPANGVFHYFSS
                     GKTDQKSSEALYDEINITDNYRTDLKQAVKKQLQDLYKTRPEAWEENKKRVREEIIKQ
                     FGISEDVFNKLQQKMPQKINSLIEEQVLTPYILRP"
     gene            complement(2021..2266)
                     /locus_tag="ACFB2Q_001838"
     CDS             complement(2021..2266)
                     /locus_tag="ACFB2Q_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918806.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGLDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            2378..2806
                     /locus_tag="ACFB2Q_001839"
     CDS             2378..2806
                     /locus_tag="ACFB2Q_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC0918807.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVLGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            2818..3294
                     /locus_tag="ACFB2Q_001840"
     CDS             2818..3294
                     /locus_tag="ACFB2Q_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC0918808.1"
                     /translation="MPIEIERRFLIENDKWRQYADEPLLLKQGYLSVEKERTIRIRIA
                     GKRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEVD
                     VFLGENSPLVVAEIELPAENADFDRPDWLGREITSDGMFTNAYLSKHPFSSWKNAV"
     gene            complement(3324..3812)
                     /locus_tag="ACFB2Q_001841"
     CDS             complement(3324..3812)
                     /locus_tag="ACFB2Q_001841"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC0918809.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEVALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(3880..4935)
                     /gene="aroG"
                     /locus_tag="ACFB2Q_001842"
     CDS             complement(3880..4935)
                     /gene="aroG"
                     /locus_tag="ACFB2Q_001842"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC0918810.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
     gene            complement(5097..5324)
                     /locus_tag="ACFB2Q_001843"
     CDS             complement(5097..5324)
                     /locus_tag="ACFB2Q_001843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918811.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFARMNGGYCFYQSVSFSLK"
     gene            5359..5508
                     /locus_tag="ACFB2Q_001844"
     CDS             5359..5508
                     /locus_tag="ACFB2Q_001844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918812.1"
                     /translation="MKKTLLALSLAAVSAVTVADIPESNGKGEYKVFTAYVKGKATAG
                     NLKEK"
     gene            5550..5846
                     /locus_tag="ACFB2Q_001845"
     CDS             5550..5846
                     /locus_tag="ACFB2Q_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918813.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPV
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            5843..6082
                     /locus_tag="ACFB2Q_001846"
     CDS             5843..6082
                     /locus_tag="ACFB2Q_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918814.1"
                     /translation="MSGVAVYLRNKNKIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVPNTTKTKTDSEKFNE"
BASE COUNT         1552 a         1684 c         1473 g         1654 t
ORIGIN      
        1 ccgtctgcct gcgccaaaat cccttgtgca taaagtgcca acatcttatc ctgctgcgcc
       61 tgttgtaggt aaatcggcaa aataacgcgg ataccggcaa tattgtttga gaccactgcg
      121 gaatacatcg cgcgggacaa caattcggga tttttcagca gggtttctcc gtcaatctgc
      181 aaaacttttc ctttttcccg cacctgcccc ggcaccttct ccctgtcgat cggtttgacc
      241 tccgcttcat gaagcctgaa ctcgggacgg cttctcaaat ccggttcgcg cggtgtttct
      301 tcggcgtatg ccgcgcttgc caataaaggc agcagcatcc atctgtttcg tgcaggcata
      361 aatgtcttcc cacaaaaata aaaataaaaa ataaccatta tcaaccggcg gattgtatca
      421 gttttttatg ccgctgttcc aaaccctgcc gcccgatgcc gcgacaatgc cgtctgaagc
      481 cagaatcggg tttcagacgg cggcaaaaaa ccggcggttt tccgccggtt tcatcattat
      541 gccccgccct tcggggcggc agggttcgga tcggtttgcg gggcggcgca cggggccgtg
      601 caggtgtgcc gcctgtgcgt caaaacattt cccggcgcat caaaacaacc atttgaccga
      661 caatgcggct tctttgtcgc cgtccgtcct tttgccgtat ccgatgcgtg cggacatatg
      721 gcctttccaa ccggcttcaa tgccaaaccg cccttcgagc gccgtcctgc ctgccagcgt
      781 ctgtttttcg ccgtccattt ccatgccgaa agattttgac ctgtgcaaaa cattaaaagc
      841 ggcaaaaggc tgaagattga caccgttacg caaagcaaaa cgggtttttg cccgaatgcc
      901 ggcgcggatt tgccactgac cgctgccgag cagcccgacc gccgcccctc gctgtcggta
      961 aagccgccgt ttacgcccaa gtaggtaaac tgcgcctgcg gttgcaggta aaactgcata
     1021 ttactgcctt atatattgcg tccctaagaa ggggcgatta acaaaaatta acgccccttc
     1081 ttagggacgc aatatataag gagtgagtac ctgttcttct attaaagaat taatcttttg
     1141 aggcattttc tgttgaagtt tattaaacac atcttcagaa attccaaact gctttattat
     1201 ctcttcccta acccgttttt tattttcttc ccaagcctcg ggtcttgttt tgtataaatc
     1261 ctgtaattgc tttttaactg cctgctttaa atcagttcgg taattatctg taatattgat
     1321 ttcgtcatac aaggcttcag atgatttttg atcggtttta ccgctggaga aataatggaa
     1381 aacaccgttt gccggcttgt ctgaaaatga aaaatcggca gtatcgccaa catttgtagg
     1441 acgagtatcc gaaccgggct gataactgaa agcaccgaag caatcaaaag acacactgca
     1501 cgggccgcgc caaacgtggg aatttctgtc ttccgccgct tctaaaataa atttgttttt
     1561 aatctcggct tctttgacgg gatcgtacgc atccgaaccc caagcatatg gcgtgcaaaa
     1621 cagcgataaa acagataatg ttaatagatt aacccgaccg cttgcttgct ttcagtgtca
     1681 tgagaaattt tcctttgtca agtgttaaag ttataatgat tatatactat atcacactac
     1741 atcgcaatga aaaatcggga aaaacaaaaa acccctccgc cgtcattccc gcaaaagcgg
     1801 gaatctagaa ttttaatgcg gcaagaattt atcggggatg gccggggttt aaaggtctgg
     1861 gttcccgcct gcgcgggaat gacggtttag aaatcacccg aaacgagaaa aagaaccgaa
     1921 accggacaag tcggattccc gcgcaggcgg gaatgacgat aagcgtaatt tcaatccgcc
     1981 atattgtctt ttcacttgcc tgaaaacaga tttcagccgc ctatccctgc atccggcggc
     2041 tgatgtagtc ggcaatcaaa tcccgctttt cccaaaaaaa cttctgcatt ttttgttcgg
     2101 aatacagttt gcggaccaga gtgggcagac cgctgtggac gatggcggcg atttctttgt
     2161 tgccttcttc ttcctgcatt gccgtctgaa aatccaaacc cctgcctttc agatatgctg
     2221 caaggcggtt gcggatgtgt gaattgagtt cgatggattt gagcattttg cctccgtttt
     2281 gcggtaagga tttattcgat ttgtttatcc gcctgatttt atcacgtttc ccaagcggtt
     2341 tgcctgtaca ataccgcttt tcggaagggt gtgggcgatg atgttttctt ggttcaagct
     2401 gtttcacttg ttttttgtca tttcgtggtt tgcagggctg ttttacctgc cgaggatttt
     2461 cgtcaatatg gcgatgattg atgcgccgcg cggcaatccc gagtatgtgc gcctgtcggg
     2521 gatggcggtg cggttgtacc gttttatgtc gcctttgggt ttcggcgcgg tcgtgctcgg
     2581 cgcggcgata ccgtttgccg ccggccggtg gggcagcggc tgggttcacg tcaaactgtg
     2641 tttgggcttg atgctcttgg cttatcagtt gtattgcggc gtgctgctgc gccgttttca
     2701 ggattacagc aatgcttttt cacaccgctg gtaccgcgtg ttcaacgaaa tccccgtgct
     2761 gctgatggtt gccgcgctgt atctggtcgt gttcaaaccg ttttgacgga gcatcttatg
     2821 ccgatagaaa tcgaacgccg ttttttgatt gaaaacgaca aatggcggca atacgccgat
     2881 gagccgctgc tgctgaaaca gggctacctg tctgttgaaa aagaacgcac catccgcatc
     2941 cgcattgccg gaaaacgggc gtggctgacg ctgaaaggct atatttcgga aatcagccgc
     3001 agcgagttcg aatacgaaat cccgcttgcc gatgcggaaa agatgatgga aacgatgtgc
     3061 ccgtttaaga tggaaaaacg gcgttatccg gtcagatggg gcggcagcct gtttgaagtc
     3121 gatgttttcc ttggcgaaaa ctcgcctttg gtcgtcgcgg aaatcgagtt gcccgccgaa
     3181 aatgccgatt ttgaccgtcc ggactggctg gggcgcgaaa tcacttcaga cggtatgttt
     3241 accaatgcct atttgagcaa acatccgttc tcaagctgga aaaatgccgt ctgaagcggc
     3301 ttcagacggc atctgccttg cggttatccc ttgaggtaat gcacgaatgt atatgcaacg
     3361 cctttgctgc tgacacggcg ttccgtccgc tctgcttctt tccaatgcgt ccggtctatt
     3421 tctgggaaaa atgcatctcc ttccacagac aaatccactt cggttatccg caaatcggtc
     3481 gctaatggca tcgcttgtcc gtatatctgc gcgccgccca taatgacggc ttcttccgcg
     3541 ccggcgcaca atgccaatgc cacctccaaa cttgccaccg tttccgcgcc tgccgcgcaa
     3601 taatccgcct gccggctgat gacgatgttc ctccgtccgg gcaggggttt gacgggcagg
     3661 gattcccacg ttttccgccc cataatgacg ggtttgccca aggtataggc tttgaaaaat
     3721 gcgaaatctt cggggatgtg ccaaggcata gcattgcccg ccccgatgca caggttttcc
     3781 gcacacgccg caattatggt tattttgagc atattttctc cacaaaaatg ccgtctgaac
     3841 gcttcagacg gcattttatg acattctgcg tcaaaaatct caacctgcgc gcgccatacg
     3901 ttttgtgttc gcacccgcca acaatgccaa caattcttca gtcgcgcccc aaccgataca
     3961 cgcatcggta atgctcttgc cgtacacttc cggcttgtcc tgcctgcctt cgaccaaatg
     4021 gctttccacc atcacgccca tgatattgcc gccgtcctgt tccaattggt cggcaatgtc
     4081 ttgtgccact tccatctgcc gggtgtaatc cttgcggctg ttggcgtggc tgcaatcgat
     4141 catcagcttg tcggttaccc ctgccgcacg cagttgttcc gccgcctcgc tgacgtgtcc
     4201 cgcatcataa ttcggctctt ttccgccgcg caaaatgaca tgacagtcgg gattgccgcc
     4261 ggtatggaca atggcggaat gcccggcctt ggttacagac aagaaatgat gcgaatggct
     4321 cgccgcaccg attgcgtcga tcgcaatctt caaattgccg tccgtaccgt ttttaaagcc
     4381 gacggggcag gacagaccgc ttgccaattc gcggtggact tggctttcgg tcgtccgcgc
     4441 gccgattgcc ccccaagaaa tcaggtcggc ataatactgg ggtgtaatca tatccaaaaa
     4501 ttcggtagag gcaggcatgc ccatattatt cagggacaac aacaggctgc gcgcctgacg
     4561 caaaccgaaa ttgatgtcga acgtaccgtc caaatgcggg tcgttaatca aacctttcca
     4621 acccaccgtc gttctcggct tctcgaaata aacgcgcatc acgatcaaaa gctcgttttc
     4681 ataccgcttg cggagtttca acagacgttc cgcatattcc aacgccgctt tcggatcgtg
     4741 aatcgaacac ggcccgataa tgaccaacag ccgcctgtcc ctgccgtgaa ccaaatcgga
     4801 aatttcctga cgggtgcggt gaaccaagcc cgaagcctct ttggaaatcg gcagctcgta
     4861 aagatgggca atcggcggca acaactcttt aacttcttta atcttaatat cgtctgtggg
     4921 gtaatggtgt gtcatcttgc atctttcctg atgggttttt atacgcggct taggttacat
     4981 cgtttacctt gcacaaacaa gtgcaacata ttactacaaa gctattttat ttcaaaataa
     5041 ctgatatatc ttttttcata gattttaaat ttcatatttt taatatttta ggaattttat
     5101 ttcaaagaaa aagaaacaga ttgataaaaa caatacccgc cattcattcg ggcaaaaccg
     5161 cacggaacat ccgttttccc gtttccacga caatccggta caggacaccc cgctgccgat
     5221 tccgcctgca catccggcaa aatttcatac gcaaggaaaa tgttgaaaac aaaaagaact
     5281 aattgcttga aaaaagattt aattggaatg cgggtatttc ccacactttc tccatttatt
     5341 tatcctcatt ataaaaacat gaaaaaaacc ctgcttgcac tctcacttgc agccgtgtcc
     5401 gccgttacgg tagccgatat tcccgagtct aacggcaaag gcgaatacaa agtctttacc
     5461 gcttatgtta aaggcaaagc cactgccggc aatttgaaag aaaaataacg cacaccgtcc
     5521 atcggttttc gttcaacgaa cgccacgata tgcgttttaa ggcagagtac gcccgaagca
     5581 aaaacgatgc agacgacaaa agagggaatg cacacatcgc taacggcgca ataggcaaaa
     5641 tggctgccgt gtatgccggt tatacctaca cccaaccggt cggcgaatcc acacgtttcc
     5701 gtggcggttt ggagcgtgtc caagttaaac gttatctggg agaaaaatgc tgcggctacg
     5761 cctgtccaaa aagcgaacgc ttcaccctgc aagcagatgg tgaaatccgt caaaaccttg
     5821 gcgaaggctg gtttctgaag ctatgagcgg ggtggctgtc tatctccgca acaaaaacaa
     5881 aatagcaatt gcctcccaag atgccaacct aaactccaaa ggccggttcg tcagcagcgg
     5941 tctgaatgtg ggcaaacaac tgaccggcag tctcggcgta gagtttgacc catactaccg
     6001 ccaccgcgca atccgcaagt ctgccgaatt tgtcccgaac actaccaaaa ccaaaacaga
     6061 cagcgaaaag tttaacgagt agggcttccg cgtagccgca acgttctaat tcccttacgt
     6121 ataaaaagca gcataaaaat gctgcctttt cccatttcca agggaagtat tccgtagtgc
     6181 agcttacctc attgtttcac acaaaccgaa aatgccgtct taaaaccaat tttcagacgg
     6241 catttgtccc ttaaaacacg ttttttcaag cgcgaccata ccaaacaccc acaggttgcc
     6301 ttccaccctt cgtacaacct ttgctccatc aaacatccac ttcctaaata ctgatgtata
     6361 ctg
//
LOCUS       DAXDSD010000108         6252 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXDSD010000108 DAXDSD010000000
VERSION     DAXDSD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.108"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            230..1018
                     /locus_tag="ACFB2Q_001847"
     CDS             230..1018
                     /locus_tag="ACFB2Q_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC0918815.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            complement(1064..1663)
                     /locus_tag="ACFB2Q_001848"
     CDS             complement(1064..1663)
                     /locus_tag="ACFB2Q_001848"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC0918816.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            1791..2408
                     /gene="gmk"
                     /locus_tag="ACFB2Q_001849"
     CDS             1791..2408
                     /gene="gmk"
                     /locus_tag="ACFB2Q_001849"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC0918817.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            2466..2672
                     /gene="rpoZ"
                     /locus_tag="ACFB2Q_001850"
     CDS             2466..2672
                     /gene="rpoZ"
                     /locus_tag="ACFB2Q_001850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC0918818.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            2765..4921
                     /locus_tag="ACFB2Q_001851"
     CDS             2765..4921
                     /locus_tag="ACFB2Q_001851"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC0918819.1"
                     /translation="MPAPQPSAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGDPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGTLHTLYQPKPGRFKDYIAIPKSNGYQSLHTILVGPYGLPIEVQ
                     IRTREMDAVAEGGIVGHWSYKSDSKTVDQAVLHTNQWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(4988..6172)
                     /gene="coaBC"
                     /locus_tag="ACFB2Q_001852"
     CDS             complement(4988..6172)
                     /gene="coaBC"
                     /locus_tag="ACFB2Q_001852"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC0918820.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
BASE COUNT         1563 a         1500 c         1669 g         1520 t
ORIGIN      
        1 tagggcggtg ctttatgtgt cgtcctgtgt gttgaagcat gatttgtatg ctttgtaaaa
       61 atgtttcaga cggcattgct gcaaatgccg tctgaagggt gtttgaagat gggcggatta
      121 tccgcctttt cgtgttcgcg gtcaattttg ccgggaaatt tataaggatt tatcaagtat
      181 ttgccttctt gggatacaat gcggcagttt gagtgcgaga gagaatatga tgaatcccaa
      241 aatcgtgttt ttcgacattg acgatacgct gtaccgcaaa tatacggata ccttgcgccc
      301 ttccgtgaaa acggcggtgg cggctttgcg cggcaaaggt atattgacgg cgttggcaac
      361 ggggcggtct ttggcgacga ttcccgaaaa ggtcagggac atgatggcgg aaacgggtat
      421 ggatgccgtg gtaacgataa acggacagtt tgcgctgctg cacggtaaaa ctgtgcgcga
      481 agtaccgatg gataccggtt tgatgggcag ggtttgtgcg catttggatg gcttgggcat
      541 ggattatgcg tttgtcggcg gagaggggat cgccgtgtcc gcgctgtcgg aatgcgtgtg
      601 ccgcgccttg cagcatatcg ccagcgattt ttttgccgat aaggattatt tttcaagcaa
      661 accggtgtat cagatgctgg tgtttgcgga ggaaaacgaa atgccgcttt ggtcggatat
      721 tgtggaacgg gaaggcttga aaacggtgcg ctggcacgag gaagcggtcg atctgctgcc
      781 tgcgggaatg tcgaaaacag acggcatcag aagcgtggtt gaagcattgg gtttggaaat
      841 ggcagacgtg atggcgttcg gcgacggttt gaacgatgtg gaaatgctgt ctgaagtcgg
      901 gttcggcgtg gcaatgggta acggggaaca ggcagcgaaa gaagcggcga aatatgtttg
      961 ccccggcgtt gatgaagacg gcgtgttgag gggcttgcaa gatttgggtg tgatttgaac
     1021 gtataataac aaccctgccg gtttcagacg gcagggtcgg ttttcagcct ttcatgcagc
     1081 cttcgttttg aagcagggta aataagggcg cgccacttgc gcggatattc ttgccgcctt
     1141 gaaggtcggt aaattccaaa atggcggcgg cttcgacaat ttccccgccg agtttgcgga
     1201 tcagttccag cccggcaagc attgtgccgc ccgtggcaac caaatcatcg accagcagga
     1261 cgcgcgaacc gggtttgacg gcatcggtgt ggatttccac cgcagcttcc ccgtattcga
     1321 gcgcgtagct ttgcgatacg gtttcaaaag gcagcttgcc ttttttgcgg atggggacga
     1381 agccgacgtt gagctggtag gcgagtgccg cgccgataat gaagccgcgc gcgtccaagc
     1441 cggcaacgat gtcgattttc tgatccatat agcggtaaac cagcaaatcg accaaaaggc
     1501 ggaagtattc cgcactttgc aggacgggcg tgatgtcgtg gaataagatg cctttttgcg
     1561 gccagttttc gattttgcgg attttgtcgg caagcgcgcc gacactcata gcttcgggat
     1621 gaaccagcat tgcgtgttcc aagtttgatg tttttaacgc catattgtag ccgtcagcct
     1681 gccgcttgcc aaatcaatca aaacaatagc ggcaaaatat gggcatcggc aaatatgaaa
     1741 tagggtgtcc gttgtgatac aatcggttgg aaaatataca ggtaaaaaat atgtccgctt
     1801 atcgaaaagg caacatcttt atcatttcgg ccgcttccgg cacgggcaaa accacgctgg
     1861 tgtcgcggct gttggcaaac cataacggtt tgcgcgtttc cgtgtcgcat acgacgcgcc
     1921 cgccgcgtga aggcgaagca aacggcgtac attatcactt tgtttccaaa gaagagtttg
     1981 aatcgcttat cgcgcaggag gcttttttgg aatacgcaga cgtattcggc aactattacg
     2041 gcacaagcac ggagggcgtg aatgcgttgg cggcggcagg ctatgacgtg attttggaaa
     2101 tcgacgttca gggcgcggcg caggttcgca acgcgctgcc ggaagctgtc ggtatcttta
     2161 tcctgccgcc ttctttcgac gtgcttgccg cgcgcctcaa agggcgcggg acggacagtc
     2221 gggaagttat ccaaaggagg ctgtcgaagg caaggcatga aatcgaacag tccgtattgt
     2281 tcgactttgt cgtggtcaat gacgacctgg agaaggcgga gggggatttg cttcatattg
     2341 tgaatgcctg ccgtctgaaa aggtcgcggc aactgggatt tattgcagat ttgttggaaa
     2401 attcctaaaa accgcgaaaa tatccggttt cccaatttaa atatttttga aagaaagcaa
     2461 ataatatggc acgtattacc accgaagact gtaccggaaa aatttccaac cattttgacc
     2521 tgacattggt agcggcccgt cgggcccgcc agcttgaaaa tggcaatacg ccgcttgtgg
     2581 acgatgtccg caataacaaa ccgaccgtta ccgccttaag ggaaatcgca gccgggcata
     2641 tcggtacgga actgttgacg cgcaataaat aaattctgcc ggaaacgcac gccggcacac
     2701 tctgccgccg tgcagtccga cggtttgaaa tgaaaaaaca catacatccc gaaagccgtc
     2761 tgaaatgccc gccccccaac cttccgcccc ttacgacccc ctgaccgccg aagcgcgtac
     2821 cctgcttttc cataccgcct cctacctcaa gcccgaggaa caggcggagc ttgaaaaagc
     2881 tgtcgcctat gcgtttcgcg cccacgacgg gcaaacccgc aaaagcgggg atccctacat
     2941 cacgcatccg attgccgttg cgacgcagct cgccctttgg catatggaca tacagggtct
     3001 ttgtgcaggc gtgatgcacg acgtattgga agatacgggc gtgacaaaag gggaaatggc
     3061 ggcggtgttc ggcaatactg ttgccgagat ggtggacggg ctgtccaaac tcgaaaaact
     3121 caaatttgaa gatcatgcgg aacatcaggc ggagagtttc cgcaaactga ttttggcgat
     3181 gaccaaagat gtgcgcgtca tcgttgtcaa acttgccgac cgcctgcaca atatgcggac
     3241 gctcggttcg atgcgcccgg acaaacgccg ccggattgca agggaaaccc ttgaaatcta
     3301 cgcacagatt gccaaccgca taggtctgaa taatgcgtat cgggagcttc aggatttatc
     3361 gttccaaaac ttgcatccca accgctacga gactttaaaa aaagcgatgg acaagagccg
     3421 gaagaacagg caggacgttg tcggcaaagt tttgcgcgcg ttcggccagc ggctggtagg
     3481 cgcgaatata gaggccaaaa tcaaaggcag ggaaaaaaac ctgtacggca tccatcagaa
     3541 aatgatggcg aaaaagctgc gttttgccga ggttatggat atttacggtt tccgcgtcat
     3601 tgtcaacagc attcccgcct gttatgccgc actcggcaca ttgcacaccc tctatcagcc
     3661 caagcccgga cggttcaaag actatatcgc cattccgaaa agcaacggtt atcaaagtct
     3721 gcatacgatt ttggtcggcc cttacggctt gccgattgaa gttcagatac gcacgcggga
     3781 aatggatgcc gtggcggaag gcggtattgt cggccattgg agctataaat cagattccaa
     3841 aacggtcgat caggcagtgc tgcacaccaa tcaatggttg aaaaacattt tagacttgca
     3901 ggcaagcagc gccaatgcca ttgagtttct cgaacacgtc aaagtcgatt tgtttccgaa
     3961 cgaaatctac atccttactc cgaaaggaaa aatcctaact ttgcccaaag gggcaacgcc
     4021 tgtcgatttt gcttatgcgg tgcataccga cattgggcat aaaacagttg ccgcgcgtgt
     4081 caataatgtg atgatgccgc tgcgtacgaa gctcaaaacc ggtgattctg ttgaaattat
     4141 cacatccgaa cacgccaaac ccaatcccgc gtggttgaat ttcgccgtgt ccggcagggc
     4201 gcgcagtgcc atacgccaat atattaaaaa tcttaaccgg cacgatgcgg tcgttttggg
     4261 agagagcctc ttacaaaaag ccttgtccag tttgctgccc aaagatgtcc tgctttcaga
     4321 cggcatcaaa gaaaaatacc ttgccgatct caacgacaaa cagacttcat ttgaggaagt
     4381 gctgtacaac gtcggtatgg ggcataccct gcctgtttat gtcgccatgc atatcgccga
     4441 attggcaggg gagcatttcg gcagcgaggt caggctcagt tccattaaag ttgatggtca
     4501 ggaaagcggg catattcatt ttgcagagtg ctgccaccct gttcccggcg attccatccg
     4561 tttgctgttg gttaagggaa aaggcatgat tatccatagg gatacctgcc cgacgttgtt
     4621 gaagtccgat cccgaacagc agctggatgc agactgggaa aatatgaacg ggcagaacta
     4681 ccgtgtcggg cttcaagtcc aatcggaaga cagccacggc ctgttggcat taatggcgca
     4741 agcgatttcc gattccggtg cggacatcga gtcggtcgaa acaccgtcta aatcccagtc
     4801 gggaacggaa ggttttgtcg aattcaaatt cttattgaaa gtcaagaatt tggatcagtt
     4861 gaatcagatt attcaaaatc tgcacagcat cccatatatc cgcaaagtca tcagaagttg
     4921 aagcaggtaa aacgggttgc ctgttaatca aaaggcaacc cgttttatgg tttatccgtt
     4981 caattgctta tttgttcaaa tatacggcaa gcctttcaac aatccgcatt gccgcttcat
     5041 ctttacttgt ttcggggaaa gacaactccg catcatcatc tatgataaca atttggttgg
     5101 ttgtcttgcc catcgcgatt gaaacatcat tggcaactat caccggtatt tttttcttaa
     5161 tacgtttttc ccgcgcatat gccattacat tctccgtttc agcggcaaaa ccgatgcaga
     5221 acggcgggtt cggcaatgag gcaatagaag ccaaagtatc ggggttctca tccaattcga
     5281 tggataacgg tttggcattt ttatcttttt tgaatttttg cgtactcctg tttttaacct
     5341 tatagtctga gacggcggca acagaaataa aagcatcttg tttttcaatc aaacgatgta
     5401 ctgcgcgatg catatcttcg gcactgacgg cttgaaccgt atcggatatg ccgaaaggca
     5461 gcgtggtttg aagctgtccg tgaatcaggc tgatttccgc accggcggca cggcacgccc
     5521 gcgccaaagc tacgcccatt tttccgctgg aggtatttgt gatgcccctg acagggtcaa
     5581 tggcttcaaa tgtcgcacct gcggtaatca agattttctt gtcccttaaa attttcggtg
     5641 tccacaagtc tggaagcaaa tccagcaatt cggcaggttc cggcatcctt cccataccat
     5701 tttctctgca agcctgttcg cccaagcccg gcatatagac agtaatgccg tctgaaacca
     5761 gttgtgcgat attccgttgg ttggcaggat tcagccacat ttccacattc atcgcgggag
     5821 cgatggcaag agggcatttc cgtgcggctg ccagattggt cagtaggtta tctgccacgc
     5881 cgttacagat ttttgccacg gtattcatac ttgccggcgc aatcagaaaa acatccgcat
     5941 tccgggtcag gttgatatgt tccataccgt ttgaaccgtt gccgccgtgc gtgtcggtca
     6001 gaacgggatt gccgctcaaa gcctgaaaag tcagcggaga aacaaattca gttgccgagc
     6061 ggctcataac caccgtaacc gaatgcccct gttttttcag cagtcgcacc aactcgcaag
     6121 acttatacgc cgcaatactg cccgttacac ctaaaagaat atgtttgccc attttgacaa
     6181 tttctaatta cgaagaaaaa acagcagcca ttctataaca aagtacggat acattagtta
     6241 ccgtttggat ac
//
LOCUS       DAXDSD010000109         6241 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXDSD010000109 DAXDSD010000000
VERSION     DAXDSD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.109"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            89..391
                     /locus_tag="ACFB2Q_001853"
     CDS             89..391
                     /locus_tag="ACFB2Q_001853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918821.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(473..2560)
                     /locus_tag="ACFB2Q_001854"
     CDS             complement(473..2560)
                     /locus_tag="ACFB2Q_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFC0918822.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHIIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAIFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2563..3828)
                     /locus_tag="ACFB2Q_001855"
     CDS             complement(2563..3828)
                     /locus_tag="ACFB2Q_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFC0918823.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(4549..4830)
                     /locus_tag="ACFB2Q_001856"
     CDS             complement(4549..4830)
                     /locus_tag="ACFB2Q_001856"
                     /inference="COORDINATES: protein motif:HMM:NF021923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFC0918824.1"
                     /translation="MSNDENLLFSQPHLTKVNIMQVLTLDEIQQVSGAACNWRDFSKN
                     TIGSALGGAAGGAIVGSFAGGIGAIPGAKFGAIGGAITGSVQYGSTCWW"
     gene            complement(5051..5287)
                     /locus_tag="ACFB2Q_001857"
     CDS             complement(5051..5287)
                     /locus_tag="ACFB2Q_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFC0918825.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGVSYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGFSKKW"
BASE COUNT         2006 a         1101 c         1077 g         2057 t
ORIGIN      
        1 ggcttagacg actttttctc atagggataa ttctaactta atttgaattt ccctagttat
       61 ctaggacagc cccttaaaaa atatcccttt gaaagatttt gatgaagttt gtgaaaagtg
      121 ggagaaatcc tttaaagaaa atggaaaaga aattgaatat aatcaaaaat attcaagaat
      181 ttatggtgaa gttcgtggag ctggctttaa tggcgatatc ggagaaaagc tacatcgttg
      241 tgccgtgatg gaatgggctg aaaatgagac cgtgaaatta gcccaaaaat gggaacaaga
      301 gcagaaaaaa caacaaattc aacagaaaaa ggaaactgaa aaatcgccaa aacacaaagc
      361 cagtcgtgat gattgggaaa tggaacgtta aaccgctatt ttcatgtatt ctattaaatt
      421 atttgaaaaa agacttcact ttttgttaaa gaaaaatgaa gtcttgttct gttcaaccta
      481 aattcagaat tttatctgcc gattgaattg tttcttggcg gtgtgcaacc attattttca
      541 taataccaag actttttagg ttatggttaa ttttttgttc attttctaca tctaaatggc
      601 tacttgcttc gtctaaaaat agaattttgg gtcgtttata caatgcacga gccaagataa
      661 ctctctgctt ttgtccacct gataagatat ttcccatatc gccaatcaag gtctcatagc
      721 ccattggcat tttaagtata tcgtcatgta tttgtgccat ttttgcacat tgttcaatga
      781 gctccatatt tgggctttca tcaaaaaatg aaatattttc cccaatagaa cctgcaaaaa
      841 ggacatcatc ttggcgaaca atcccgctca atcccctaat aaaggatgga gaaacttgat
      901 atatatcatg cccattaata ctaactgtac cagtttcagg ttttaggcta cctgttaaaa
      961 tgtttaacaa agtggacttc ccccgaccag attgtcctgt taaaacaact gcttcattat
     1021 ctttgaattc caaattaatg ttttcaaaaa gatatggctc attatcagca tatctgaatg
     1081 agacgttttc aactttaaga accagttgtt cattatctaa tttaggtata tgattatact
     1141 taataatttc agtttctgtt tcatttaaag taatgtcagc caaacgttca gcatgaagcc
     1201 ctaacatttt gatttggatg tattggtcaa cgagagaagc tgttctgctt tcaaattgcc
     1261 ctttataagc caaaaaagcc atcagaacac cgactgtaaa tgaaccatct aaaattgcgc
     1321 ttgcaccaag ataaattatg ataatatttt ccatgctaaa caacagttta tttgaaaatt
     1381 caaataaagc agagagttta tctgttgtca gcttggtatt gactgtattc acaaataggc
     1441 tcatccaagt gccatgtctt tgataatgtt tatcaaataa tttaactgat tggataccac
     1501 gaatggtttc catgaaatat gagttttgtt tggcttcatg aacaatattt tcttctgttg
     1561 catttcttaa tgggtaatat gcaagccaac gaattagtat gtacaaaaca agtgttaaaa
     1621 gaacaatcag cgataattga gtgctgtaaa ttgtcattaa cacgaaagta aaaatagcca
     1681 ttaagctatt taaaactaaa acaaaaaaag tagaagttag tgtttcttgg atatgatcta
     1741 ttgaaccaaa tcttgaaatc acatctccta aatgtcgttt actgaaatag tcattaggta
     1801 agtcaagtaa ccttttaaat atattggctt tccattgtaa attaagagtt gtagataggt
     1861 gcatacctac ccatgcttgt aacaggctaa ttaactgttg caggatagcc agtaaaccaa
     1921 atcccaaagt aagggtcagt aataaatttt tatcagcagt tacaatgata tggtctatta
     1981 cccattgcat aaagaatgga ctaaccaatg caaagacttc caaagaaata gctaatataa
     2041 gcatttgaat taaagagcgt tttaagcctg aaatccccct taatagagat aatattttga
     2101 ttttctttgt ttcttttttc tcttcaaaat gggtattggg gaataattct agggcaatcc
     2161 ctgtgaattt ttgtgaaact tcgtccattt tgatttttcg cataccgaca gcagggtcca
     2221 taatgacgat actgtctttg gaaatggaac aaagtacaac aaaatggttt aagttccaat
     2281 ggagaatgca gggtagttgt aaatttgaca gctcatctaa ctctaaacgc aaagctcgtg
     2341 gcgttaaatt catttcattg ccaaatctca tgatgtctgc aagatttgcg ccctttaatg
     2401 acagggtgta tttttggcgt aacgttctta aatcagtgtg aaagccataa taggacaaga
     2461 tggatgtcag gcatgctaaa ccacattcag caacttctgt ttgcagaatg acaggtagct
     2521 ttttgttaaa tccaaaggac agtcttgata aataatccat ttttaattga tttttcctga
     2581 aatgctgtaa agtgggtcaa gtacccattc gtacaatttt tttcgttcat ggagaatatc
     2641 tgcttctaaa atcatgccaa tttgaagcgg cttgttttct ccgtatgctt taatcgtttg
     2701 tttttccaat ttaactttca caagataggc aggttcattt aataaggttg ggttagtgaa
     2761 aatgatacct aaacctgata gctcttgttt accgagagca gttctggcaa ctgaaataat
     2821 ttctcctgtg gcatgtccaa atttttggta agggtacgct tggtaacgta aaacaacttt
     2881 atctttcggt ttaataaaac caacagcttt actgggtatg taaagattgg cgaccaattc
     2941 agtttgttca gggacaatgc ttaacagcaa tttagacggt tcaacttgtt gccctatatc
     3001 aacattaatt gttgaaatat aacctgattt actagctcgt atggtttgtt cggatttcaa
     3061 atcaaaatcc aaaatttctt ggttcatttc cgtaatcgca cggttgagtt ggctcaattc
     3121 ggttttatgg cgttcaggca ggctgcttaa tgtgattttc tgttcatcaa gttccctgat
     3181 tgcattattt tgttcacgtt ttaggctctc caaacgtgag cgttgttcca ataaatggct
     3241 ttcggcggtc atcttatctt gttgggatac tgcgccttga ctggctaaaa acttgttctt
     3301 gttaagggtt ttttccgcta aacgaatttg acgattttgc cctgtaattt gctgtttaat
     3361 attctctaat tgattgttta aacgatgaat gttgttatga acattttttt gctcattttg
     3421 atgaatgcgc tttaaacgtt ccaattcttg taatgccaaa gtttttttaa ggttggcttc
     3481 tgctgccaat ttggcttgta cgtttccttt ttcgccaaaa cgcgatgtgg aaagtttgaa
     3541 caatggttcg ccagctttga caaagttacc atcttcaaca aatttatgcg taatcgtgcc
     3601 gatatcggaa gagtaaacac gaaccacccc catagttgga agtaattgac cttcaacggt
     3661 tgttttattg gtatagctac caaaaatcaa aaagataatg atacacagag caatgagaaa
     3721 agcgcaaaaa gtcagaaata aaaaagagaa tggacgggtc aagataacct gacctgtcca
     3781 cttattttgt tgggctacaa agacttcttt tcggaataag tgggacattt attattattt
     3841 cttaaaaaaa atggatagaa aaacaccaat aattaaatac ttatgcctaa taacataaat
     3901 ttgctattga ttgatgtttc tattttaatt acattaaatt taaccaataa tccagaaatg
     3961 gttgcagcta aaatgcaaaa gacagtggcg taaatggttt cagtattcac aatttttctc
     4021 tttcaaaatt gttcaatcta aatagaaaaa ggtaacttca atacatattg ctgtaactga
     4081 aattaccttt tccttaatga tgtaattatc ttatggcttc atcatgccac gaccgatatt
     4141 gtatccgcca gcaacagaga aaataccagc tcccattgcg tcaacccagc ctactggtcc
     4201 tgtccacttg ttccgttgag ctacaaagac ttctggtctg aaaaatgagt gattgttaga
     4261 catgtgtact tatttatgtt tctttgattg ggataaattt gtaaaaacaa aaaaaaggca
     4321 agttgttatt atagaagaac ttaaaatgct taacatactc attgaggttt gtaccacata
     4381 atgagatacg agataatttg ttgggagtaa aatcaaaaaa aagattaaat atttaaaata
     4441 atcactatat tttttcatta tttgttcctt tttgtattta agttcttatg aaaaactagg
     4501 gtattttgaa tagaaaatat caaaataccc tagttttatt aaggaatatt accaccaaca
     4561 agtgcttcca tattgtacag aaccagtgat tgcaccacca atagctccga atttcgcacc
     4621 tggaatagca ccaataccac ctgcaaatga accaacaatt gccccaccag ctgctccacc
     4681 taatgcacta ccaatggtat tttttgagaa atcacgccag ttacaagcag caccagaaac
     4741 ttgttgaatt tcatccaaag tcaaaacttg cataatattc acctttgtta agtgtggttg
     4801 agaaaaaagt aaattttcat cgttactcat tatttgcaat catgcatata ctgtaaccaa
     4861 gcactatatt cagttggatc atagttttta ctactgggtt gtctgatatg actattggca
     4921 ctattggcac aactgggatt agcaacttta ttgtgagtac ctaatggatt gatatttcct
     4981 gtaaagtggc tattgcatgc gcttaatgag catagattta ttaatgtcag aaaggataaa
     5041 acaaaaaatc ttaccatttt ttactaaaac caaaagtagg tttcgaaaac tgtacaccaa
     5101 gaccttgtac atatccaaat tgcttgctaa cagaaaactc tactccacta ttgttagggg
     5161 aataggaaac accaatttta ccaacagcaa ccatatcttt tttacctagg ttgccgccac
     5221 cctgtgcaga gatatggaat ttgccacctg acacttcaac taattcagag gtgtgtaact
     5281 ctttcatgat tttctcctgt aaagaagtat ggttggaaat gtaaatcaga atcaatcttt
     5341 cttaagttta atttaatact tttttttgca agtattttat tttaaattaa atcaatattt
     5401 taaaaatatt tacttgtata ctgaaaaaga aactcctaat ggcggagtgg ggcttttgtt
     5461 taagtaagca ttgaaaaaat ttaaatttcc cattatgatt aacttaatgc aagagctttt
     5521 tcaggacgta caaaccagcg aatgatgtgg gcaggttggt tgtcgctaac caatttcagt
     5581 agctcaatcg tcagattggt atccgaccct atcaagctaa ggagaggctg cggctactac
     5641 acaaagcacc tgaaattatt gggtgctttt gtctttttat ggcattgtac caatttgtgt
     5701 accacgaggg cttgttcgcc caaacgcggt aacaactgaa tacacacttt ccccctcaac
     5761 ttctttgcgt tctaaaacga ctgtcccaat ccgagaatag acaatcatag gacgatgatt
     5821 gccccttaca tcactaaatt cactaaaaat ttttgcgcct gattgaaaaa tcatttccac
     5881 ataaaagggt tacacctgtt tcatcgtggc ttaatccaaa ccgcgttaaa tctgctttgt
     5941 gttctgccaa aatgtgaatc acaccaaagc cgtgattaac ctttttgtgt tcgccgtgtt
     6001 ttaaaacaat ttgacctgcc gggaagtctg ctctattgtt cggacaataa ggggaatata
     6061 ccaattactt taactacatc attttttgac atttttcttg cctttcaatt taaaatccaa
     6121 aatcatactg ccataattta gcaatccaaa aaaatttagg cagcagtaat ggtttcgtat
     6181 tttagaaaac gaaacttggt ttggtgtttt gaagcatttc agtacacaaa catggaattt
     6241 t
//
LOCUS       DAXDSD010000110         6195 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXDSD010000110 DAXDSD010000000
VERSION     DAXDSD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.110"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            70..405
                     /locus_tag="ACFB2Q_001858"
     CDS             70..405
                     /locus_tag="ACFB2Q_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918826.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEADNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            598..3288
                     /gene="glnE"
                     /locus_tag="ACFB2Q_001859"
     CDS             598..3288
                     /gene="glnE"
                     /locus_tag="ACFB2Q_001859"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC0918827.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNGIIADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISLRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNRLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            3712..5919
                     /gene="uvrD"
                     /locus_tag="ACFB2Q_001860"
     CDS             3712..5919
                     /gene="uvrD"
                     /locus_tag="ACFB2Q_001860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC0918828.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
BASE COUNT         1555 a         1900 c         1556 g         1184 t
ORIGIN      
        1 cccggttata ttctcactgg gaacactatt tgaacaacgg cggcgggcag ggttaaaacc
       61 ggcaatccga tgccgtctga agcgttatcc ggcgttcaga cggcattttg ttttccgaca
      121 gtttataaac tgtcgttgtt tcttgacaga aacaacgacc ttatttattt gaaacgattg
      181 gaggacatga ttatgggttt ttggggtagt gtgggaaaag cagcaaaagc agtaggcgag
      241 ggattgattg aagccgacaa tgagcataag gcattgaaaa tggaatatgc ggagaaatca
      301 agtgaggagc tgcatgaaat cgtaaagagt gatggttttt ttaaaaattc cacacgggag
      361 aaaggtgcgg cttatgctgt tttaaaagag cgtggcgagg tttgaacggg aaacggcggc
      421 atttgccgct gttttttatc ggcaggcatc cgtccgaata tcggggcaag gtttcagacg
      481 gcatcgaagg ttgctatgat atagtggctt gactttaaac cggtacggct ccgccttgcc
      541 ctgatttgaa gttcatccgc tatcacattc ccgccatcct ttcaaacgga atccgaaatg
      601 tccgaccacc gcctcgacac cgcccgccgc cattccctgt tcctcgcccg ccagctcgac
      661 aacggcaaac tcaagcccga aattttcctg cccatgctgg acaaggcgtt gacggatgag
      721 gatttccaag cctttgccga ctgggacaaa atccgcgcgg aagaaagcga ggaagaattg
      781 gcgcggcagt tgcgcgagtt gcgccgttat gtggtgtcgc agattatcgt gcgcgatata
      841 aaccgtatca gcgatttgaa cgaagttacc cgcacgatta cgctgtttgc cgattttgcc
      901 gtcaataccg cgctggattt cgcctacgcc tattatcagg acatgtacgg cacgccgatc
      961 gggcgttata ccaaatcgcc gcagcatttg agcgtggtgg cgatgggcaa ggcgggcggc
     1021 tatgagttga acgtgtcttc cgacatcgat ttgattttcg tctatcccga atcgggcgac
     1081 accgacggca ggcgcgaacg ggggaatcag gagtttttca ccaaagtcgg gcagaaactg
     1141 attgcattgt tgaacggcat tattgccgac gggcaggtgt tccgcgtcga tatgcggctg
     1201 cggccggacg gtgattcggg cgcgttggta ttgagcgaaa ccgcgctgga gcaatatctg
     1261 attacgcagg ggcgcgaatg ggaacgctat gcgtggtgca aaggccgcgt agtcacgccg
     1321 tatccgaacg acatcaaatc gctggtgcgc cccttcgtgt tccgcaaata tctggattac
     1381 ggcgcgtatg aggcgatgcg caacctgcac cgccaaatcc gcagcgaagt cagcaaaaaa
     1441 ggcatggcgg acaacatcaa actcggcgcg ggcggcatcc gcgaggttga atttatcgcc
     1501 cagattttcc agatgatacg cggcggccaa atgcgcgcgc tgcaactgaa aggcacgcag
     1561 gaaacgctga agaaaattgc cgagatgggc attatgccgt ctgaaaacgt cgaaaccctg
     1621 cttgccgcct accgcttcct gcgcgatgtc gaacaccgcc tgcaatactg ggacgaccgg
     1681 caaacccaaa ccctgccgat ctcgcccgaa cagcggcaac tgctcgccga aagcatgggt
     1741 ttcgacagtt atgccgcttt ttcagacggc ctcaatgttc atcggaacaa agtcaatcag
     1801 ttgttcaacg aaattttgag cgaacctgaa gaacaagcgc aaagcaatag cgaatggcaa
     1861 tgggcatggc aggaaaaacc cgacgaagaa gaacggctag gccgtctgaa agaacacggg
     1921 ttcgatgccg aagccgtcac cgcaaggctc gaacaaatct gccacggaca taaataccgc
     1981 cgcctttccg cacacgccca gccgcgcttt gacaccattg tgccgctgtt cgtacaggcg
     2041 gcggcagagc aaaacaaccc gacagatacg ctgatgcggc tgttggattt tctcgaaaac
     2101 atcagcctcc gctccgccta tctcgccttc ctcaacgaac atccgcaaac cttggcacaa
     2161 ctggcgcaga ttatgagcca aagctcgtgg gtggcggcgt atctgaacaa atatccgatt
     2221 ttgttggacg aactcatcag cgcgcagctt ttggataccg cgtttgattg gcaggcactc
     2281 gccgccgccc tttcagacgg catcgaagcc tgcggcggcg ataccgaagc gcaaatggac
     2341 acgctgcgcc atttccaaca cgcccaagtc ttccgcctcg ccgtccaaga cctcgccgga
     2401 ttgtggacgg tagaatccct ctctgaccaa ctctccgccc tcgccgacac cgtcattgcc
     2461 gccgcccttt cgtgcgcgtg ggcggatatg cccaaaaaac accgcgacac cccgcaattc
     2521 gccgtcatcg gctacggcaa gctcggcggc aaagaactcg gctacgcctc cgacctcgac
     2581 ttagtctatc tttacgacga tccccatccc gaagcaggcg acgtgtacag ccgcctcgcc
     2641 cgccgcctga ccaaccggct ttccgccgcc accggcgcgg gcagcctcta cgaaaccgac
     2701 ctgcgcctgc gccctaatgg cgacgccggt ttcctcgcgc acagcatcgc cgcctttgga
     2761 aaataccagc gtgaaaacgc atggacatgg gaacaccagt ccctcacccg cgcccgcttc
     2821 atctgcggca cacccgaaat tcagacggca ttcgaccgca tccgcaccga aatgctgact
     2881 gccgaacgcg accaaaccgc cttggcaggc gaaatcatcg aaatgcgcga aaaaatgttc
     2941 cccacccatc cgcctgccga cagcaacgtc aaatacgcgc gcggcggcgt ggtcgacgtc
     3001 gaatttatcg tccaatatct gatacttgcc catgcccgcc agtatccgca actcttggac
     3061 aactacggca acatcgccct attgaacatc gccgccgact gcggtttgat tgacaaaacc
     3121 ctcgccggac aaagccgcac cgcctaccgc ctctaccgcc ggcagcagca caacaccaaa
     3181 ctgcgcgacg cggcaaaaac cgaagtaagc gacgaattgc tgtcccatta cggcaatgtc
     3241 aggaaattgt ggcgggaagt gttcggggag gaagcggcaa ccgcctgaac aaaaaatgcc
     3301 gtctgaagcc tgacaatccg ggtttcagac ggcatttgtt tttggctgtg tctgaacaat
     3361 cggtacgatt tcggttgttt gcaaattgaa aggcggttat tcctatgttc aggcggcaaa
     3421 ccgaaatatt ttccccaacc gatagggcga tatcaaagcc tattcgaata ccgcctttac
     3481 ggggaagggg ctttccgagc ttgaccatgg tcaggctcgg caaatcagcc cgcatcctgt
     3541 cgatgcgtcc gtccaaatcg gtttggaacg ccgcaatgtg atatccgcct atcaaggttt
     3601 tgcgtgcgtg tgtcggcgag ggctttgcca aaaccgaaat caaaatcaag ttgtcaggca
     3661 tccttccgtt tctattaaaa tagcgcattc catttttcag acggcatcct tatgtttccc
     3721 gaccaatccg cccccaatct gctgcaaggc ttgaaccccg aacaactctc cgccgtaacc
     3781 tggccgccgc aatccgccct cgttttggca ggcgcaggca gcggcaaaac gcgcgtgctg
     3841 accacgcgta tcgcatggct tttgcaaagc ggacaagcca gcgtacacag cattatggcg
     3901 gtaacgttta ccaacaaggc cgccaaagag atgcaaaccc gactcggcgc aatgattccc
     3961 atcaacgtcc gcgctatgtg gctcggcacg ttccacggtt tgtgccaccg ctttttgcgc
     4021 ctgcaccacc gcgacgcagg cctgccgtct tcctttcaaa tcctcgacag cggcgaccag
     4081 ctttccctca tcaaacgcct gctcaaaagc ctcaacatcg ccgaagaaat catcgcgcca
     4141 cgttcgctgc aaggctttat caacgcgcaa aaagaatccg gtttgcgcgc ttccgtcttg
     4201 ggcgcgcccg atccgcacac aagccgcatg attgggtgct acgccgaata cgacaaaatc
     4261 tgccaacgcg aaggcgtggt cgatttcgcc gaactcatgc tccgcagcta cgaaatgctg
     4321 caaagtaacg aaatcctgcg ccggcactac caaaaccgct tcaaccacat tctggtcgac
     4381 gagtttcaag acaccaacaa actgcaatac gcctggctca aactcatggc gggcggtaac
     4441 gcagcggtat ttgccgtcgg cgacgacgac caaagcattt accgcttccg cggcgcaaac
     4501 gtcggcaaca tgaccgcact gatggaagaa ttccacatcg acgcgcccgt caaactcgaa
     4561 caaaactacc gctccgtcgg caacatcctc gccgccgcca acgccgttat cgaaaacaac
     4621 gacgaacgtc tcggcaaaaa cctgcgcacc gacgccgaag caggcgacaa aatccgctac
     4681 tactccgcct ttaccgacct cgaagaagcc cggttcatcg tggatgaaac caaagccctc
     4741 gaacgcgaag gctgggattt ggacgaaatc gccgtcctct accgcagcaa cgcccaatcg
     4801 cgcgtcatcg aacaaagcct gttccgcagc ggcatcccct acaaaatcta cggcggcctg
     4861 cgcttttacg aacgccaaga aatcaaacac gccctcgcct acctgcgcct cgccgtcaat
     4921 cccgacgacg acaacgccct cttgcgtgtc atcaacttcc caccgcgcgg catcggcgcg
     4981 cgcaccgtcg aaaaccttca gacggcctca aacgaacaag gcatcaccct ctggcaggca
     5041 gcctgcaacg ccggcgcgaa agccgccaaa gtcgccgcct tcgtccgcct gattgaagcc
     5101 ctgcgcaacc aagtcggaca aatgcacctg tccgaaatca tcgtcggcat cctcaaagac
     5161 agcggtctga ccgagcacta ccgaacccaa aaaggcgaca accaagaccg cctcgacaac
     5221 cttgacgaac tcgtcaacgc cgccatcgag ttcaaacccg aagacagcaa ctttgaaacc
     5281 ctgcccgaaa acatttcaga cgaccccgcc ttccccatcc tcgccttctt gagcaacgcc
     5341 gccctcgaat ccggcgaaaa ccaagcaggg gcaggcgaaa aagccgtcca actgatgacc
     5401 gtccacgccg ccaaaggctt ggaattcaac gccgtcttcc tcaccggtat ggaagagggg
     5461 cgtttcccca gcgaaatgag ccttgccgag cgcggcggcc tcgaagaaga acgccgcctc
     5521 atgtacgtcg ccatcacccg cgcccgcaaa cgcctctaca tcaccatggc acagcaacgc
     5581 atgctgcacg gacaaaccca attcggcatc gcatcccgct tcgtcgaaga aatcccgcca
     5641 gaagtattgc actacctgtc cgtcaaaaaa cccgccttcg acagctacgg caacacgcgc
     5701 caaaccaccg tacaacggga caaaatcatc gatgacttca aacagccgca aacctacgca
     5761 ggcttccgca tcggacaaaa cgtccgtcac gccaaattcg gcaccggcgt gattatcgat
     5821 gccgcagaca aaggcgaatc cgcccgactg accatcaact tcggcaaaca gggcgtgaaa
     5881 gagctggata ccaagtttgc gaaattggaa gcgatgtaaa tttgaaatgc aggccggata
     5941 ttcgtatccg acaaaaaaac atttgacgcg tttatcgttt ccgaaaaacc gctgttggaa
     6001 atgtcggatt cgagaatccg acctacggca aaaaaccgta gcaaggacaa ggcaaatagt
     6061 cgtctgaaaa cgggaagggc aattttgccg cagccgccgc cgtcattccc gcgaacctac
     6121 attccgtcat tcccacgaaa gtgggaatcc ggaacgcaaa atctaaagaa accgttttat
     6181 ccgataagtt tccgc
//
LOCUS       DAXDSD010000111         6075 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXDSD010000111 DAXDSD010000000
VERSION     DAXDSD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.111"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..191
                     /locus_tag="ACFB2Q_001861"
     CDS             <1..191
                     /locus_tag="ACFB2Q_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918829.1"
                     /translation="LRAEQSVQAPFETRQISYAWRESGSPEETVLTAQTADSPDLPVQ
                     AVLNLTHRSHNGKPLILP"
     gene            252..1286
                     /gene="pyrC"
                     /locus_tag="ACFB2Q_001862"
     CDS             252..1286
                     /gene="pyrC"
                     /locus_tag="ACFB2Q_001862"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC0918830.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASENGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="ACFB2Q_001863"
     CDS             complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="ACFB2Q_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC0918831.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(1868..2344)
                     /gene="ribH"
                     /locus_tag="ACFB2Q_001864"
     CDS             complement(1868..2344)
                     /gene="ribH"
                     /locus_tag="ACFB2Q_001864"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC0918832.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(2391..2714)
                     /locus_tag="ACFB2Q_001865"
     CDS             complement(2391..2714)
                     /locus_tag="ACFB2Q_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC0918833.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2882..3601
                     /gene="rnc"
                     /locus_tag="ACFB2Q_001866"
     CDS             2882..3601
                     /gene="rnc"
                     /locus_tag="ACFB2Q_001866"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC0918834.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            3648..4571
                     /gene="era"
                     /locus_tag="ACFB2Q_001867"
     CDS             3648..4571
                     /gene="era"
                     /locus_tag="ACFB2Q_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC0918835.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            4724..6075
                     /locus_tag="ACFB2Q_001868"
                     /pseudo
     CDS             4724..6075
                     /locus_tag="ACFB2Q_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692696.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
BASE COUNT         1423 a         1468 c         1770 g         1414 t
ORIGIN      
        1 aattgagggc ggaacagtcg gtgcaggcac cgtttgaaac caggcaaatc agctatgcct
       61 ggcgggaatc gggcagccct gaagaaaccg tattgaccgc acaaaccgcc gattccccgg
      121 atttacccgt acaggcagta ttgaacctaa cgcaccgttc ccataacggg aaacccctaa
      181 ttctcccctg acttcagacg gcataaagtc ggcatgccgt ctggaaacca aaaatctaaa
      241 aaggaacaac catgcaaacc ctgaccatca tccgacccga cgatatgcac ctgcacctgc
      301 gcgacggcga cgcgctcaaa gccgttgccc cttataccgc ccgccagatg gggcgcgccg
      361 tcatcatgcc caacctcaaa ccgccagtcg tcagcgtagc cgacgcgctt gcctacaaag
      421 cgcgcattat ggcggcgttg cccgaaggca gcgcgtttga gccgctgatg acgctttacc
      481 tgaccgacca agccacgccc gaactcgtgc gcgaagccaa agccgccggc atcgtcgcct
      541 tcaaactcta ccccgcaggc gcgaccacca attccgattc cggcgtaacc gacctgttca
      601 agctcatccc cgtgttggaa gaaatggcaa aacagggcat cctgttcctc gttcacggcg
      661 aagtaaccga ccccgaaatc gacattttcg accgcgaagc cgcctttatc gggcgcgtga
      721 tgaaacccgt tttggcgcaa gtgccgaatc tcaaagtcgt gttcgaacac atcaccaccg
      781 ccgaagccgc ccgcctcgtt ttggaagcgg gcgacaacgt tgccgcctcc gtgaccccgc
      841 aacacctcct gctcaaccgc aacgacctct tggtcggcgg tgtgcgcccc catcatttct
      901 gcctgcccgt cctcaagcgc gaaacccacc gtcaggcatt ggtcgccgcc gttaccggcg
      961 agaaggcgca caaattcttc ctcggcaccg actccgcgcc gcacgccaaa tccgccaaag
     1021 aaaacgcctg cggctgcgcc ggtatgttca gcgcgatgac cgctatcgag ctttacgccg
     1081 aagtgtttga aaaagcaggc gcgttggaca aactcgaagc cttcgcctcg gaaaacggcg
     1141 caaggttcta cggcattccg gaaaacgccg acacgattac ccttgtcaaa caaagccaaa
     1201 ccgttcccgc aagcgtccct tacggcgacg gcgaacttgt cccgatgcgc gcgggcggcg
     1261 aaatcggctg gacggtgcag tattgattga acggtcaagc aaaatgccgt ctgaaaggtt
     1321 ttcagacggc atttgtgtat tttccgattc ggattttccg agtatcaacg gcgtttgggt
     1381 tcgtctgggc ggatttgggc ggcgagtttg tcgaggatgc cgttgacgaa tttgtgcccg
     1441 tccgtgccgc cgaaggtttt ggtaacttcg atggcttcgt tgataatgac ggggtagggc
     1501 gtttcgggca tagcggaaag ctcgtggcag gcggtcagca aaacggcgcg ttcgatgggg
     1561 ttgaggtctt tttcgtccct gtcgagcagc gggcggattt tttggatgta gtccgctgcg
     1621 ttggtttgtg tgccgaagaa gagtttgttg aacaattctt cgtccgcttt ggcaaagtcg
     1681 gacatttcgc ggatgttttt agcaatttcg ggcgcggcgg tgcggttgat aagggattgg
     1741 taaacggctt gtacggcaag ctcgcgggaa cggcggcggg ctgttttcat gatttttcct
     1801 tgaaacggtt gggcggcacg gtatgccgtc tgaaacggaa agggcgcatc ggtgtacgcc
     1861 ctgtttgtta ttcttcgtct tcaaactgtt cttcgagcag aaggttgacg aggttggcgc
     1921 attctacggc gactttggcg gcatccgagg ctttttctcc aatccgttca attgcctgcg
     1981 cgtcgttttc ggtggtcagg acggcgttgg caatcgggat gttgtagtcg agtgcgacgc
     2041 ggccgatccc tgcgccggat tcgttggcaa ccagctcgaa atggtaggtt tcgccacgga
     2101 tgacgacgcc gatggcaatc agtgcgtcaa atttttcgga agaggcaaag ttcatcagcg
     2161 cgatggggat ttcaagcgcg ccgggtacgg tggcgacggt gatgttttcg tctgctacgc
     2221 ccaattcttg gagggtgcgg cagcagactt tgagcatttg gctgccgatt tcgttggtaa
     2281 agcgtgcctg tacgatgccg atgcggaggt gtttgccgtc gaggttgggg gcgatggtgt
     2341 tcattgggtg tcctttggta ttcggggttt cggaatgccg tctgaaggtt tcagtcttgc
     2401 ggctgccagt cggcaacggt ttggaatgtg ccgtcttcgg caagctccca cgcgctgcct
     2461 tcgggttggg aaagcagtgc ggcggtttca gggttggttt tggtgatgtc ggcgaggctg
     2521 acgatgctga agttgtcggg gtcgtcggtg tattcgtcgg tttcgtcgcc gctgaagaaa
     2581 cgccagccgc tgtcgttttc aaaaacgggg gcttcgcggt aaaggaagcc gacgggccgg
     2641 ttttgtttgg cgacggtgtt ggtggcgatg cagcggtcga gtgccgagga aagtgcttgt
     2701 gcaaatgcgt tcattgcggg aatacgttgg gggggaaact tgcggatttt accacgattc
     2761 ccgcgttgtc ggcagacggc ggcggtttgg tggtacaatg tgcgccgttt gcagccttaa
     2821 ggtgtttctg tatttttgga gtatggaaac gcattcgggc tgttttttgc ggaagacggt
     2881 aatgaaagac gatgttttga aacggcaggc acatactgcg atacagaaaa agttaggcta
     2941 cgcgttccgc gatatgtcgc ttttgcggcg ggctttgacc cacaggagcc atcatgcgaa
     3001 gcacaacgaa cggttcgagt ttgtcggcga ttcgattttg aattatacgg tggcgcggat
     3061 gctgtttgac gcgtttccga agttgaccga gggcgagttg tcgcggttga gggcgagtct
     3121 ggtcaatgag ggcgtgctgg cggaaatggc ggcggaaatg aatgtcggcg acggtctgta
     3181 tttgggggcg ggcgagttga agagcggcgg cttcagacgg ccttcgatac tggcggacgc
     3241 gatggaggcg atgtttgccg ccgtcagctt cgatgccgat ttcaacacgg cggaaaaggt
     3301 ggtgcgccat ttgtttgcag aacgcgtccg gcgcgccgat ttccaaaatc aggcaaaaga
     3361 cggcaaaact gctttgcagg aggcgttgca ggcgcgccgt ttcgccttgc cgaaataccg
     3421 catcgaagag caaatcggcc atgccaacga cagtatgttt gtcatttcct gcgatttggg
     3481 cgaactgggt ttcgtgtgcc gtgccaaagg gacgagccgc aaggcggcgg aacaggaggc
     3541 ggcgaaagag gctttgaaat ggctggaaga gaagctgccg ctgaagaaga aaaagaaatg
     3601 aggcggcgcg tgaatatgcc gtctgaaatg tggatatgaa agcgaatatg gatattgaaa
     3661 ccttccttgc aggggaacgc gccgccggcg gataccgttg cggcttcgtg gcgattgtcg
     3721 gtcgtccgaa cgtgggcaaa tcaacgctga tgaaccatct catcggtcag aaaatcagta
     3781 ttaccagcaa aaaggcgcag acgacgcgca accgcgtaac ggggatttat accgacgata
     3841 ccgcgcagtt cgtgtttgtc gatacgccgg gctttcaaac cgaccaccgc aacgcgctca
     3901 acgacaggct gaatcaaaat gttaccgagg cgctcggcgg tgtggatgtg gtggttttcg
     3961 tcgtggaggc gatgcgcctt accgatgccg accgcgtcgt gttgaaacaa ctgcccaagc
     4021 acacgccggt cattttagtg atcaacaaaa tcgacaagga caaggcgaaa gaccgttacg
     4081 cgctggaggc gtttgttgcc caagtgcgcg ccgaatttga atttgcggcg gcggaggcgg
     4141 tcagtgcgaa acacggtttg cggattgcca acctgttgga gctgctcaag ccgtatctgc
     4201 ccgaaagcgt accgatgtat cccgaagaca tggttacgga caaatcggcg cgttttttgg
     4261 cgatggaaat cgtgcgtgaa aaactcttcc gctatttggg cgaggagctg ccttatgcga
     4321 tgaacgtcga agtggagcag tttgaagagg gagacggttt gaaccgcatc tacatcgccg
     4381 ttttggtcga caaagaaagc caaaaggcga ttttgatcgg taaaggcggg gagcgtttga
     4441 aaaaaatttc caccgaagcg cggctggata tggaaaaact gtttgataac aaagtatttt
     4501 tgaaggtctg ggtcaaagtc aaatccggtt gggcagacga cattcgcttc ctgcgcgagc
     4561 tgggtttgta gtttttcttg ctgaacttta cgcaaatgcc gtctgaacag gtttcggacg
     4621 gcattttgtt tcaatcggga atatctttgt taaaaacggg ttgatattat ctgtgcatat
     4681 taatataatg ataattatta ttaattaaat aggggggata gggatgcgta aactgaatta
     4741 ttacggtatt gccttgctgc cgttgatgct ggcatcctgc ggcggcaatt tcggcgtgca
     4801 gcctgttgtc gaatcaacgc cgaccgcgta ccccgtcact ttcaagtcta aggacgttcc
     4861 cacttccccc ccctgccgag ccttcggtag aaaccacgcc ggtcaaccgg cccgctgtcg
     4921 gtgcggcaat gcggttgttg aggcggaata ctgcttttca tcgtgaagat ggcacggcaa
     4981 ttcccgatag caaacaagca gaagaaaagc tgtcgtttaa agaaggtgat gttctgtttt
     5041 tatacggttc aaaaggaaat aaacttcaac aacttaaaag cgaaattcat aaacgtgatt
     5101 ccgatgtaga aattaggacg tcagaaaagg aaaataaaaa atataattat aaatttgtgg
     5161 atgccggtta tgtatatact aaaaacggaa aagatgaaat cgaacagaac tcaggtggta
     5221 agcggtttac ccaccgtttt ggttatgacg gttttgtata ttattccgga gaacgtcctt
     5281 cccaatcttt accgagcgcg ggaacggtgc aatattccgg caactggcaa tatatgaccg
     5341 atgccaaacg tcatcggaca ggtaaggcgg ttgccagtga cgatttgggt tatataacat
     5401 tttatggtaa cgatattggt gcaacttctt atgcggctaa ggatgccgac gacagggaaa
     5461 aacatcctgc cgaatatacg gttgatttcg acaaaaaaat cctaaagggc gagctgatta
     5521 aaaatcagta tgtgcaaaag agaaatgatc caaaggaacc taaaaaaccg ctgaccattt
     5581 acaacatcac tgccgattta aacggcaacc gctttaccgg cagtgccaaa gttaacaccg
     5641 aggtgaagac gagacacgct gataaagaat atttgttttt ccataccgat gccgatcagc
     5701 ggcttgaggg cggttttttc ggcgataacg gagaagagct tgccggacgg tttatcagta
     5761 acgacaacgg cgtattcggc gtattcgcag gcaaacaaaa aacaaacgca tcaggcacaa
     5821 atcctgctat gccgtttgga aaacacacca aaatcttgga ttctctaaaa atttccgttg
     5881 acgaggcgac tgatgaaaat ccccgtccgt ttgaggtttc cactatgccc gattttggtc
     5941 atcctgacaa acttcttgtc gaagggcgtg aaattccttt ggttagcaaa gagaaaacca
     6001 tcgatcttgc cgacggcagg aaaatgaccg tcagtgcttg ttgcgacttt ttgacctatg
     6061 tgaaactcgg acgga
//
LOCUS       DAXDSD010000112         5716 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXDSD010000112 DAXDSD010000000
VERSION     DAXDSD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.112"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(139..411)
                     /locus_tag="ACFB2Q_001869"
     CDS             complement(139..411)
                     /locus_tag="ACFB2Q_001869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918836.1"
                     /translation="MPSETPNRLQTASVADENSKSAYRHSCENSSSHPSGCRHLYPEI
                     PSFPRKWESRNEKQQEFIGNNRNRTDWIPACAGMARKSGLWVGLVE"
     gene            complement(369..524)
                     /locus_tag="ACFB2Q_001870"
     CDS             complement(369..524)
                     /locus_tag="ACFB2Q_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918837.1"
                     /translation="MPSESPSDGIGFPKHSAPLSSCRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(541..2337)
                     /locus_tag="ACFB2Q_001871"
     CDS             complement(541..2337)
                     /locus_tag="ACFB2Q_001871"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC0918838.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLSGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEQDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSARPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2796..4049
                     /locus_tag="ACFB2Q_001872"
     CDS             2796..4049
                     /locus_tag="ACFB2Q_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC0918839.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QLALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSCVYLWVFLILCIASATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLVAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRIRPSGYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGREKTGNAELFAWN
                     IWVAGSGLAVIFWFDGAMAELLKFAMIAAFVSAPVFAWLNYRLVKGDKRHRLTAGMNA
                     LAIVGLLYLAGFAVLFLLNLTGLLA"
     gene            4204..5715
                     /gene="lysS"
                     /locus_tag="ACFB2Q_001873"
     CDS             4204..5715
                     /gene="lysS"
                     /locus_tag="ACFB2Q_001873"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC0918840.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLNNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDGFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         1197 a         1509 c         1672 g         1338 t
ORIGIN      
        1 ttttcttgga atagcgcatt aaaaagagta tgagaatgga gacataaaaa attccggcga
       61 atgtccgtgc gccgttcccg tccgtcattc ccgcacaggc gggaatccgg atttgtccgc
      121 acggaaactt atatttcgtc attccaccaa tcccacccac agcccacttt tccgcgccat
      181 tcccgcgcag gcgggaatcc agtccgttcg gtttcggtta tttccgataa attcctgctg
      241 cttttcattc ctagattccc actttcgcgg gaatgacggg atttcagggt aaagatggcg
      301 gcagccggag gggtgtgaag agctgttttc gcaggaatga cgatatgcgg atttgctgtt
      361 ttcatccgct accgatgccg tctgaagcct gtttggcgtt tcagacggca ttgggttttt
      421 tgtgcatttg ccgcaaaacg gatcggtgtc tgcttgcggc aagacgataa cggtgcggaa
      481 tgttttggga aaccaatgcc gtctgaaggg ctttcagacg gcatttttgt accgtgccgt
      541 ttaacgcgcc agcggttcgg tgcgtttgat cagccacgct tttgccgcgc cttcggtcag
      601 cggctcgagg cggcggcgga cttcggcgtg gtagcggttg acccagtcga tttcgccgtc
      661 ggtcatcagg gcggtgtcca tcaggcgtgt atcgatgggg cagagggtca gggtttcaaa
      721 acagaggaag ctgccgaact cggtttcctg cgggtcggcg acggcttggt tggcggcgag
      781 gttttcaatg cggatgcccc atttccccgg acggtagagg ccgggttcga tggaggtaac
      841 catgcctttt ttcatggcgg tttcgggcgt ggcgggggcg gcgaaggcga tgcgctgcgg
      901 gccttcgtgg acgttgagga aatagcctac gccgtggccg gtgccgtgtc cgtagtcgca
      961 ttgcgcctgc cacaggggtt tgcggcaaat cgcgtcaatc agcggcgagg ggatgttttc
     1021 ggggaacacg gcttcggcaa gcgcgatatg ggctttgaga acgagcgtgt tgtcgcgttt
     1081 ttgttctgct gtgggcgtgc cgacggggac gacgcgggtg atgtcggtcg tgccgccttt
     1141 gtattgcgcg ccggagtcga tgagcagcag accgttgccg ctgatggtgc tgtggctttc
     1201 gggtgttgcg ctgtaatgcg gcagggcgcc gttggcgttg aagcctgcga tggtgtcgaa
     1261 actcaaggac acaaaacccg ggcgcgcgct gcggtggcgg taaagcatcg tatccacgtc
     1321 gatttcggtc aggctgccgc ctttgccgat gatgtcttca aactcggcga agaagccgca
     1381 caacgccgcg ccgtcttgtt ccatcgcttc gcggatgcgg gcgatgtcgg cttcggattt
     1441 gacggatttg aagaaggtgg acgggttgat gccttcaatc aggcgcgcgc tttcgggcag
     1501 gcgcacgagc gtactgacgg cggttttgtt cggctcgatg agcagcgcgc cgccgatttg
     1561 cgcgagtttg tcggcaactt gggcgtaagg ctcgaccgtg atacctgcgg tttgcagcgc
     1621 ggcggcggct tcggcgttca gacggcattg ttcggtaaac aggacggcac tgtctttgcc
     1681 gatcagcagg aaggacacga aaacagggtt gaaaggcacg tcgctgccgc gcaggttggt
     1741 cagccaagcg atgtcgtcaa gcgaggaaac caagtggtaa tccgcgcctt tttccgccat
     1801 aactgcgcgc acgcgggcga gtttctcggc ggcggtttcg gagacgtagg cgtggtcgtg
     1861 gatgaacacg gtttcggcgg ggatagcggg acggcttgtc cacacttggt caagcaggtc
     1921 gtcggggtgt tggatgcgga tgtttttggc ggcgagcgat tgcgccaaag tgcgtttgcc
     1981 ggtgagcgac accatatcgg aagggatgcc gacggcagcg ttttcgggca ggtttgccgc
     2041 gagccattcg ttgtacggcg gcacttgccc gcttttttgc agctcaatgc cgctgccgga
     2101 aagctgtttg gcggcttgtt cccaatagcg gctgtccacc catacgcccg cttcatcggc
     2161 ggtaacgacg aatgtgccga ccgagccggt aaagcctgac aattcgcgcc gcgcctgcca
     2221 atgctcgggc aggtattcgg ataggtgggg gtcggcggaa gggatgacga gtgcgtccaa
     2281 gccttgcgcc ttcatggctt cgcgcaatgc ggacaggtaa ttcgatacgg tattcataac
     2341 agttcctcca atcgggtttc gcggcttcag acggcatgga cggaaatctg aaatgccgtc
     2401 tgaaaaacat aaccatatta tcaaatcgcc agcctgctgc aaaacggacg ggctgatcgg
     2461 atggtgcgtg tttgcacgaa aaaaagcgat gtaaggttgt ttgttgtaaa atttcaggca
     2521 taaaatgtcg tttcatgcct tcttttggaa gtaatacgga aaatatagtc aggtttcagc
     2581 atgaagttct aatagtgtgt gttggcaggt atttggatta cgttacaata cgcgctttca
     2641 tcaaaatttc cctaccgggc ggtatcctgt ttttgaagac ggtaggattt gggtttgttt
     2701 ggggtcaatc ccgcccaaat tggagcaata gatagggctg tgtccgaata tgcggctctg
     2761 tatgttgaaa cacagttaaa aatacggaag actttatgtc cgaacaacat atttcgactt
     2821 ggaaaagcaa gatcaacgca ttgggaccgg ggattatgat ggcttcggcg gctgtcggcg
     2881 gttcgcacct gattgcctcg acgcaggcgg gcgcgcttta cggctggcag ctcgcgctca
     2941 tcatcatcct gaccaacctc ttcaaatacc cgtttttccg cttcagcgcg cattacactc
     3001 tggacacggg caagagtctg attgaaggtt atgccgagaa aagctgcgtt tatttgtggg
     3061 tttttttgat tttgtgtatc gcctccgcca cgattaacgc gggcgcggtc gccattgtaa
     3121 ccgccgccat cgtcaaaatg gcgattccct cgctgatgtt tgatgccggc acggttgccg
     3181 ccttgattat ggcatcctgc ctgattattt tggtgagcgg acgttaccgc gctttggatc
     3241 gcgtttccaa aatcatcatc gttactttga gtatcgccac gcttgtcgcc gccggcatcg
     3301 ctatgtcgcg cggtatgcag atgcagcccg attttatcga gccgacaccg tggacgcttg
     3361 ccggtttggg cttcctgatc gcgctgatgg gttggatgcc cgcgccgatt gagatttccg
     3421 ccatcaattc tttgtgggta accgaaaaac aacgcatccg gccttccggc taccgcgacg
     3481 gcattttcga tttcaacgtc ggttatatcg ccagtgcggt tttggctttg gttttccttg
     3541 cactgggcgc gtttgtgcaa tacggcaacg gcgaagcagt gcagatggcg ggcggcaaat
     3601 atatcgggca attgattaat atgtatgccg taaccatcgg cggctggtcg cgcccgctgg
     3661 tggcgtttat cgcgtttgcc tgtatgtacg gcacgacgat taccgttgtg gacggttatg
     3721 cgcgcgccat tgccgagccc gtgcgcctgc tgcgcggcag ggaaaaaacc ggcaacgccg
     3781 aactctttgc ctggaatatt tgggtggcgg gcagcggttt ggcggtgatt ttctggtttg
     3841 acggcgcaat ggcggaactg ctcaaatttg cgatgattgc cgcctttgtg tccgcccctg
     3901 tgttcgcctg gctcaactac cgcctcgtca aaggggacaa acgccacagg cttaccgccg
     3961 gtatgaacgc ccttgccatt gtcggcctgc tctacctggc cgggtttgcc gttttgttcc
     4021 tgctgaacct gacggggctt ttggcatagt ttccaaaaca cggatgccgt ctgcaagatt
     4081 ttcagacggc atcgcccttt cttggaaaaa aacaccgaca tcgcgtaaaa tacgcgcaaa
     4141 ttttgtgatt cggtcaggtc gtctgaaaca gactgcgcct gatttatttt ttcggaaaac
     4201 cctatgagcg aacaaaacca tccgcaaacc gagccgcagt tggacgaaaa ccaaatcatc
     4261 gccctgcgcc gcgaaaaact gaacaacatc cgccaacagc gcaacgctta ccccaacgat
     4321 ttcaagcgcg acagctttgc cgccgatttg caggcgcaat acggcgaaat cggcaaagaa
     4381 gaactcgacc cgcaggccgt tcccgttaaa attgccggcc gcatgatgct caaacgtcaa
     4441 atgggcaaag ccagcttcgc caccattcaa gacgttaccg gtcaaatcca gctttatctg
     4501 aacaacaaag gcgtgagtca ggaagttttg gacggcttca accactggga cttgggcgac
     4561 atcgtcggcg cggaaggcac tttgttcaaa accaaccacg gcgaactaac cgtgcgtgtg
     4621 tccgacatcc gcctgctgtc caaatccctg cgtccgctgc ccgacaaaca caaaggcttg
     4681 agcgatcagg aaaccaaata ccgccaacgc tacgtcgatt tgattgccaa tgaagaatcg
     4741 cgcaatacct ttatcaaacg cagccaaatc atccaatctg tgcgtaattt tatggtgggc
     4801 gagcattatc tcgaagtcga aaccccgatg atgcacccga ttcccggcgg cgcgacagcg
     4861 aaacccttcg ttacccacca caatgccttg gacattccgc tctacctgcg catcgcgccc
     4921 gagctgtatc tgaaacgctt ggtggtcggc ggcttggaac gcgtgtttga aatcaaccgc
     4981 agcttccgta acgagggcat gtccgtgcgc cacaaccccg aatttacgat gattgaattt
     5041 tacgaagcct tctccgacta cgaacgtatg atgcagatgg cggaagacat catccgcaac
     5101 gcatcgcgca cggtaaacgg cacggcaaac atcacttaca acggcaaaga agtcgatttg
     5161 gaaagcccgt tcgaacgtct gaccatcctc gaagccatca aaaaatacaa tccgcactac
     5221 accgacgagc agttgaacga tgcggaatgg ctgaaaaaag aaatcgtcaa acacggcgaa
     5281 agcctgccgc cgtccccggg catcggcagc ctgcaactcg ccctgtttga aggttgcgcc
     5341 gagggcaaac tgtggaatcc gacctttatt gtcgattacc cggtcgaagt ttccccgttg
     5401 gcgcgcgctt cggatacgaa acaaggtctg accgaacgct tcgagctttt cgttgtcggg
     5461 cgcgaactgg caaacggcta ttccgagttg aacgaccccg aagaccaagc cgaacgcttc
     5521 aaatcgcaag tggcgcaaaa agatgcgggc gacgacgaag ccatgcacta cgatgccgac
     5581 tacatccgcg cgatggaatt cggtttgccg ccgaccggcg gttgcggcat cggcatcgac
     5641 cgcttagtga tgctgctgac cgatttgcaa accatccgcg atgtgattct gtttccgcag
     5701 atgcgtcccg agtaat
//
LOCUS       DAXDSD010000113         5275 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXDSD010000113 DAXDSD010000000
VERSION     DAXDSD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.113"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            362..1042
                     /gene="tsaA"
                     /locus_tag="ACFB2Q_001874"
     CDS             362..1042
                     /gene="tsaA"
                     /locus_tag="ACFB2Q_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC0918841.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            complement(1194..2198)
                     /gene="gap"
                     /locus_tag="ACFB2Q_001875"
     CDS             complement(1194..2198)
                     /gene="gap"
                     /locus_tag="ACFB2Q_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC0918842.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            2629..5223
                     /gene="mutS"
                     /locus_tag="ACFB2Q_001876"
     CDS             2629..5223
                     /gene="mutS"
                     /locus_tag="ACFB2Q_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC0918843.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
BASE COUNT         1299 a         1468 c         1283 g         1225 t
ORIGIN      
        1 gatatgtcat atccaccccc tccattttag ctatacctaa ggtactcctt gctagaagct
       61 gtaatccgat tttattaccc aaaatttcag atctcacctc ctgtaagtga gatccaaaag
      121 gaaagataac aacctgactt gggtgtaata tacgggattc cctcttcgtt ataacgaacc
      181 gttatgcttc ttttttatca atactccaat gcctttttaa gagcgtcttg gacacgagga
      241 ttctgtgcta ctttatttca acgcgattta aatgccctgt attataacaa attactaccc
      301 gaaagcggta aaaccggctg tgataagata aggtttttcc aaaaaactta tccacaatct
      361 tatgacttat accattaccc ccatcggcac cgcccgctcg ccctacaaac agaaattcgg
      421 catcgcccgc cagcccggtt tggtctccgc cgcagaagcc tgcatcgagc tgaatcccga
      481 atttaccgca gacagcgtgc gcgggttgga agatttcgat tatgtgtgga taagttttat
      541 cttccacggc gtattggatg aaggttgggc gcaaatggtg cgcccgccac ggctcggcgg
      601 caaacaaaaa atgggcgtgt tcgccacgcg cagcccccac cgccccaacc atctcggact
      661 ctctctcctt aaactcgaac gcatcgaaac cggcaaaccc gtccgcctct attgcagcgg
      721 ctcagacctg ctggacggca cgccgattgt ggacatcaag ccttatatcc cctttatcga
      781 atccaaaccc gatgccgcat ccggtttcgt cagcggcaaa cccgtagagt tggaagtcgt
      841 ttggcaggaa aacatcggcg cggaaaattt atctgcaaac accaaaaacc ttatcagcca
      901 aagcattgcc caagatccgc gccccgccta tcagaatatt cccgaacgga tttatgtgat
      961 gaatattgcg gattacgaag tcaggtttca aatcgaggaa aaccgcgcga cggttattaa
     1021 tctttcccca accccgcttt aaatcgggca aaatccggtt ttgccgcata gcagttgaac
     1081 aaacggctgt tgtttgttcg ccataagccg caatatcaag ttacagataa acaatgccgt
     1141 ctgaacgcaa tgtgttcaga cggcatttac ttatccacag gtttgttcaa gccttagatt
     1201 ttgcctgcga agtattccaa agtacggacg agttggcagg tgtaggacat ttcgttgtcg
     1261 taccaggcaa cggttttcac cagttgtttg ccgcccacgg tcatcacgcg ggtttgggtt
     1321 gcgtcaaaga gcgagccgta ttcgatgccg acaacgtcgg aagaaacgat ttgatcttcg
     1381 ttgtagccgt aagattcgct ggaggcggct ttcatcgcgg cgttgatttc ttctttggtt
     1441 gcagggcgtt cgaggacgga aaccaattcg gtcagcgagc cggtggcaac agggacgcgt
     1501 tgggcggagc cgtcgagttt gccgttcaat tcggggataa ccagaccgat ggctttggcg
     1561 gcgccggtgc tgttgggcac gatgttgagc gcggcggcgc gggcgcggcg caaatcgcct
     1621 ttgcggtgcg gcgcgtcaag ggtgttttgg tcgccggtgt aggcgtggat ggtggtcatc
     1681 aggccttcga ctacgccgaa ctctttttgc aggactgccg ccatcggggc aaggcagttg
     1741 gtggtacagg aagcggcgga aatgacagtt tcgctaccgt ccaagatgtc ttggtttacg
     1801 ccatatacga cggttttcac atcattgccg ccgggtgcgg aaatcacgac tttgcgcgcg
     1861 ccggcacgga tatgtgcttc ggctttggtt ttgttggtaa agaaaccggt acattcgagg
     1921 acgacatcca cacccaactc gccccaaggc aattcttcgg gattcggatt ggcgaaaact
     1981 ttgatttctc tgccgtttac cacgatggca tcgtctttta attcggcagt accttggaaa
     2041 cggccttgtg tgctgtcgta tttgaaaagg tgcagcagca tttcggcagg ggtcaggtcg
     2101 ttgacggcgg cgacttcgat gccgtgggct ttttcaattt gacgcaatgc gaggcggccg
     2161 atgcggccga aaccgttaat cgctactttg atgctcatat tttgtctccc ggttgtgaaa
     2221 taaagtccga tgcctgtatt gtattctgaa ataaaactac attccactat tacataaaat
     2281 tacttgcgtt ttgtttgacg caggtgaatt tcgctatttc aaaaacgcgc attcaatatt
     2341 tgattttaaa attttaatga tgattttgat gattgccgac ctgcttgtgt ataagtagca
     2401 aatatccaat attttcatta cctttttgtt aaataaattt gagtttaagg cttgccgtat
     2461 aagacagata agcgtggatg ttttttgact taataatatt cttatggata actttgccgt
     2521 tttcctattt gtctccacaa ctttattgac aagcttacgg tcagtctcat tccgtcgaag
     2581 acaaaacctt ttgctacaat accgttttcc taataataag gcagccccat gtccaaatcc
     2641 gccgtttccc caatgatgca gcaatacctc ggcataaaag cgcaacatac cgacaaacta
     2701 gtgttttacc gtatgggtga tttttacgag ctgtttttgg atgacgcggt ggaagcggcg
     2761 aaattgttgg acatcaccct gaccacacgc gggcagatgg acggcgtgcc gattaaaatg
     2821 gcgggcgttc cgtttcacgc ggcggaacag tatctggcgc gcctggtcaa gttgggcaaa
     2881 agcgtggcga tttgcgaaca ggtcggcgaa gtcggcgcgg gcaaagggcc ggtggagcgc
     2941 aaagtcgtgc gcatcgtaac gcccggcacg ctaaccgatt ccgcattact ggaagacaag
     3001 gaaaccaacc gcatcgttgc cgtgtctccc gacaaaaaat acatcggttt ggcgtgggca
     3061 tcgctgcaaa gcggcgaatt caaaaccaag ctgacaactg cggataaatt gaacgacgaa
     3121 ctggcgcgcc tgcaggcggc ggaaattttg ctgccggaca gtaaaaacgc accgcaactt
     3181 cagacggcat cgggtgttac acgcctgaac gcgtggcagt ttgccgccga cgcgggggaa
     3241 aaactgctga cggaatattt cggctgccaa gatttgcgcg gcttcggttt ggacagcaaa
     3301 gaacacgccg tttcgattgg cgcggcaggt gcgctgttga actatatccg cctgacgcaa
     3361 aacctgatgc cgcaacattt ggacggcctg tcgctcgaaa ccgacagcca atatatcggc
     3421 atggatgccg ccacgcgccg caatctcgaa atcacgcaaa ccctctccgg caaaaaaacg
     3481 ccgaccctgt tttccatact cgacggctgc gccacccaca tgggcagccg cctgttggca
     3541 ttgtggctgc accacccttt acgcaaccgc gcccacatcc gagcgcgcca agaagccgta
     3601 accgcgctgg aaagccaata cgaacccctc caatgccatc tgaagagcat tgccgacatc
     3661 gaacgtatcg ccgcccgcat cgccgtcggc aatgcccgcc cgcgcgattt ggcatcttta
     3721 cgcgacagtc tgttcgagtt ggcacaaatc gacttgtccg caaccggcag cagcctgttg
     3781 gaaaccctta aagccgtttt ccccgaaacc ctgccagttg ccgaaaccct caaagccgcc
     3841 gtgatgcccg aaccttccgt ctggctgaaa gacggcaatg tcatcaacca cggttttcat
     3901 cccgaactgg acgaattgcg ccgcattcaa aaccatggcg acgaattttt gctggatttg
     3961 gaagccaagg aacgcgaacg taccggtttg tccacactca aagtcgagtt caaccgcgtt
     4021 cacggctttt acattgaatt gtccaaaacc caagccgaac aagcacctgc cgactaccaa
     4081 cgccggcaaa ccctcaaaaa cgccgaacgc ttcatcacgc cggaactgaa agcctttgaa
     4141 gacaaagtgc tgactgctca agaccaagcc ctcgccttag aaaaacaact ctttgacggc
     4201 gtattgaaaa accttcggac ggcattgccg cagcttcaaa aagccgccaa agccgccgcc
     4261 gcgctggacg tgttgtccac attttcagcc ttggcaaaag agcggaactt tgtccgtccc
     4321 gagtttgccg actatccggt tgtccacatc gaaaacggcc gccatcccgt tgtcgaacag
     4381 caggtacgcc acttcacagc caaccacacc gacctcgacc acaaacaccg cctcatgctg
     4441 ctcaccggcc ccaatatggg cggcaaatcc acctatatgc gccaagtcgc gctgattgtt
     4501 ttattggcac acaccggctg tttcgtgcct gccgatgccg ccacaatcgg gcctgtcgat
     4561 caaatcttca cccgcatcgg cgcatcggac gacctcgcct ccaaccgctc caccttcatg
     4621 gtcgaaatga gcgaaaccgc ctacatcctg catcacgcca ccgaacaaag cattgtttta
     4681 atggacgaag tcggacgtgg tacttccact ttcgacggcc tcgccctcgc gcacgcaatc
     4741 gccgaacacc tgctgcaaaa aaacaaatcc ttcagcctgt ttgccaccca ctatttcgag
     4801 ctgacctacc tgcccgaagc ccacgccgcc gccgtcaata tgcacctttc cgcgctcgaa
     4861 cagggacggg acatcgtgtt cctgcaccaa atccaacccg gtcccgccgg aaaaagctac
     4921 ggcatcgccg tcgccaaact cgccggcctg cctgtgcgcg cattgaaagc cgcccaaaag
     4981 catttgaacg gactggaaaa ccaagccgcc gcaaaccgtc cccaactgga tattttcagt
     5041 accatgccgt ctgaaaaagg agatgaaccg aatgtggact gctttgtgga taaagcagag
     5101 gaaaaacatt ttgaaggtat attggcagca gccctggaaa acctcgatcc cgacagcctg
     5161 accccgcgcg aagcattgtc agaactgtac cgtctgaaag atttgtgcaa atccgtatct
     5221 taatttccgt tggcggagca gcatcaaacc atatggaaaa atctgtggat aaact
//
LOCUS       DAXDSD010000114         5210 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXDSD010000114 DAXDSD010000000
VERSION     DAXDSD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.114"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            96..917
                     /locus_tag="ACFB2Q_001877"
     CDS             96..917
                     /locus_tag="ACFB2Q_001877"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC0918844.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1003..1665)
                     /locus_tag="ACFB2Q_001878"
     CDS             complement(1003..1665)
                     /locus_tag="ACFB2Q_001878"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC0918845.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELGRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1703..2530)
                     /locus_tag="ACFB2Q_001879"
     CDS             complement(1703..2530)
                     /locus_tag="ACFB2Q_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC0918846.1"
                     /translation="MKPDIYALPERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVRVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            2913..3536
                     /gene="lolA"
                     /locus_tag="ACFB2Q_001880"
     CDS             2913..3536
                     /gene="lolA"
                     /locus_tag="ACFB2Q_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC0918847.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            3809..4948
                     /locus_tag="ACFB2Q_001881"
     CDS             3809..4948
                     /locus_tag="ACFB2Q_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC0918848.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKMRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPTKELMEKSFIVSPKSAESVKLGVKLWQGLKAGK"
     gene            5043..>5210
                     /locus_tag="ACFB2Q_001882"
     CDS             5043..>5210
                     /locus_tag="ACFB2Q_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC0918849.1"
                     /translation="MNLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKI"
BASE COUNT         1218 a         1409 c         1443 g         1140 t
ORIGIN      
        1 gggtgtaatc cgaggtaggg gcggctgggg tgcttctccc ttgtctgccg ctgctgttat
       61 gatgatattt ttattctgta tttaaggagg gggtgatgag cagtccgcgc caagtgtttt
      121 atatttccga ccgcaccggt ctgactgccg agaatatcgg cgaggctttg ctgaaccagt
      181 ttggcaatct gtcgttcaaa cgccatacgc atccgtttgt cgatacgccg gaaaaggcgc
      241 gcgcggtggt ggagaaggtc aatcggagcc ggcaggaaaa cggtcagcgt ccgattgcgt
      301 ttgtcagtgt ggttgatgac gaaatccgcc ggattattaa aggggcggat gcttttcaga
      361 ttaatttctt tgagactttt ttgggactgt tggagaagga actcaatacc gaagcaacgg
      421 tatccgggca ggggcatcac agtatcggca atacgaagcg ttacgatgcg cgtatggagg
      481 cagtcaattt ttctttgaac catgatgacg gggtcagcga taagaacctt caggaggcgg
      541 atgtgattct gatgggcgtg tcccgttcgg gcaaaacgcc gacctgcctg tatctggcgt
      601 tgcaatacgg tatccgtgcg gcaaactatc cgctgattcc cgatgatttg gaatcggccg
      661 atctgccgcg tatggtcaag ccgtataaag acaaactgtt cgggttgact atccagccgg
      721 aacgtttgca ggccatccgt caggaacgcc gcccgaattc ggcttatgcg cgcatcgaca
      781 cctgccgcag cgaagtggcg gacgcgcaga gtatgttcag acggcatggc attccgtttg
      841 cgaatacgac ggacaagtcg gttgaggaac tggcggtaca catccttcag gcgtgcaagc
      901 tcaaacgcag gttttgacgg gctttgattc ggtttgaagg tggaaatgcc gtctgaaatc
      961 aggtttcaga cggcagtttt atgtttgtgg ggcggatatt tttcagggct gtattttgtc
     1021 cagacattcg agcagatcga gcggcgtgcg gatgtggaaa tccgcctgcc atgagccggt
     1081 atcgtcttca tcggaaatat agccccattc ggcgaggacg gttttcatac cggcgttgcg
     1141 tccggcttgg atgtcgcgtt ccgcgtcgcc gacgtagagg gtgtgttgcg ggtcggcgtg
     1201 gatttttccg caggcgtgca gcatgggttt gatgctgggt ttgggttcgc cgcaggtgtc
     1261 gccgctgacg acggtggcgg gcggaacggc gaatccgagt ttgggaacga gtttgtcggt
     1321 gaagcgcatg ggtttgttgg tgatgatgcc ccatttgatg ccgcgtctgc cgagttcggc
     1381 gatgagttcg ttcacgccgt cgaagagagt ggtgtcttgg gcgtagcggc tgtcgtattc
     1441 ctccagatat tcggtgcgcc atgcggtata gtcggggtgt tcgggcgtga tgttcgcgcc
     1501 gagtttgagc aatcctgccg cgccgtggct ggcttgggtg cggatttcgt ccatgctttt
     1561 ttcgggcagt ccgtggcggg cgagttgggt gttgagtgcg ccgccgaggt ctagggcggt
     1621 gtcggcgagc gtgccgtcga ggtcgaacaa tacggcttgt atcatgtgtt ttcctttttt
     1681 ataaagtgtg ggacgaaagg tttcagacgg catgtttatt ttgtttcaaa ccctgctcga
     1741 aatcttccaa catatccaat tcgaagcggc tgaagcccgc tttttcccgc gcttcgatgt
     1801 tcacatagcc gcggaagata aacatatcgt aacgggcaat cagactgcgg aacagggcga
     1861 caggttccaa accgcgttcg cggcagaggt gttgatacca ccggttgccg atggcgacgt
     1921 gtcccacttc gtcgcggtaa atgatgtcca acacgccgca ggttgccgaa tcgccgcgct
     1981 gctccacctt cgcgcgtatc cccggcgtaa cgtccagtcc gcgcgcttcc aacacgcgcg
     2041 gcaccaacgc catacgcaac aaaggatcgt aggcggtttt gtatgccata tcccataaat
     2101 gattgtgtgc ctcgaaatcg ccgtaatcga agccgaaagc acgcagcctt tcgcgcatca
     2161 ggcggaagtg gtacacctct tccttcgcca cccgcaccca gtcgcggaca aactgaaacg
     2221 gcagcgtgcg gaaacggtat gccgcgtcca aagccagatt gacggcgttg aactcgatat
     2281 gcgtaatcgc gtgcagcata gccgcatagc cttctgccgt gttcattttg cgcggggtca
     2341 gttgcgaggg cgcgaccaaa accggcttgt ccggccgtcc cgcgcggggg aagtccgccg
     2401 gcggcgcgtt tgcctccgcc ccgtccgcat tttgaacggc ggcaaacgcc tcatccgtca
     2461 gccgtccttt ttcatcgggg tcgcccgaaa gcagggcgcg ttccggcaaa gcataaatat
     2521 cgggtttcat ctcaagtccg ccgtgttcgg aaaacgaata ttatagcgtt taaaaaaaac
     2581 aagatgaggc atataatctc cgcgattcgg cattccgcgc ccaaaccgtc aaatatagtg
     2641 gattaacaaa aaccagtacg gcgttgcccc gccttgccgt actatgttgt actgtctgcg
     2701 gcttcgtcgc cttgtcctga tttttgttaa tccactataa cgcggcacac aattgaaagg
     2761 gcagcgtggc gcgcctgctt tttccgagcg gtcaaaaaaa tcagccctcg gaaaacgcgg
     2821 tttgcaaaat gcaaaccgcc cgtaacgctg cccgtatgat tgttttgccg cgccgatact
     2881 ttgcgccaca cccatcccga caaggaaaaa taatgatgaa accgcacaac ctgttccaat
     2941 tcctcgccgt ttgctccctg accgtcgccg tcgcttccgc acaggcgggc gcggtggacg
     3001 cgctcaagca attcaacaac gatgccgacg gtatcagcgg cagctttacc caaaccgtcc
     3061 aaagcaaaaa gaaaacccaa accgcgcacg gcacgttcaa aatcctgcgc ccgggcctct
     3121 tcaaatggga atacactttg ccctacaaac agactattgt cggcgacggt caaaccgttt
     3181 ggctctacga tgttgatttg gcacaagtga ccaagtcgtc ccaagaccag gccatcggcg
     3241 gcagccccgc cgccatcctg tcgaacaaaa ccgccctcga aagcagttac acgctgaaag
     3301 aggacggttc gtccaacggc atcgattatg tgcgggcaac gcccaaacgc aacaacgccg
     3361 gctaccaata catccgcatc ggcttcaaag gcggcaacct cgccgccatg cagcttaaag
     3421 acagcttcgg caaccaaacc tccatcagtt tcggcggttt gaataccaat ccccaactct
     3481 cgcgcggcgc gttcaagttt accccgccca aaggcgtgga cgtgttgagc aactgatgcc
     3541 ggccgccccg atgccgtctg aacgccgccg aggcttcaga cggcattttc acgcaggcgg
     3601 aacaatgtcc cgcattaccg cccgatcggg caacggaacg gcaaatccgt gaaaattaac
     3661 ggttgcgccc cggctgtttt tgccgtttaa tgcaaacctt gctgcaccaa gggccaagaa
     3721 agccgaccgg ccgcccccac agcttccgat gcaggcggcc cgtccgtccc tgcaatgttt
     3781 tttatttttg aacgaaaggt cgaaaaccat gaaaaaaaca ctggtggcgg cggcaatcct
     3841 gagccttgcc ttgactgcgt gcggcggcgg aagcgatacc gccgcccaaa ccccctccgc
     3901 caagcccgaa gccgaacaat cgggcaaact caacatctac aactggtcgg attatgtcga
     3961 tcccgaaacc gtcgccgcct ttgaaaaaga aaccggcatt aagatgcgtt ccgactatta
     4021 cgacagcaac gaaacactgg aggcaaaagt cctgaccggc aaatccggct acgacctgac
     4081 cgcgccgtcc atcgccaacg tcggccggca aatcaaagcg ggcgcgtatc agaaaatcga
     4141 caaggcgcaa atcccccatt acggcaacat cgataaagat ttgctgaaaa tgatggaagc
     4201 cgtcgatccg ggcaacgaat acgccgtccc ctatttctgg ggcatcaaca ccttggcaat
     4261 caacacgcgg caggtgcaaa aggcattggg tacggacaag ctgcccgaaa acgagtggga
     4321 tttggtgttc aaacccgaat acaccgccaa actcaaatcc tgcggcatca gctatttcga
     4381 cagcgcaatc gaacagattc ccttggcgtt gcactatttg ggcaaagacc ccaacagtga
     4441 aaatcccgaa gacatcaaag ccgccgtcga tatgatgaaa gccgtccgag gcgacgtgaa
     4501 acgcttcagc tcttccggct atatcgacga tatggcggcg ggcaacctgt gtgccgccat
     4561 cggttacggc ggcgatttga acattgccaa aacccgtgcc gaagaagccg caaacggcgt
     4621 ggaaatcaaa gtattgaccc cgaaaaccgg cgtaggcgtg tgggtggatt cctttatgat
     4681 tccgcgtgac gcgcaaaacg ttgccaacgc ccaccgctat atcgactaca cgctccggcc
     4741 cgaggtggct gcgaaaaacg gcagcttcgt tacctacgcg cccgccagcc gtcccgcgcg
     4801 cgagctgatg gatgaaaaat acacctccga cgcatcgatt ttcccgacca aagaactgat
     4861 ggaaaaaagt ttcatcgtat cacccaaatc cgcagaatcc gtcaaactgg gcgtgaagct
     4921 gtggcaaggg ctcaaggcgg gcaaataacc ggaatccctg ccgtctgaaa cctttcgggc
     4981 ggcaggaaac ggcgtgtccg cattaagtca ggattaggat tatttagaaa gatgagatgg
     5041 atatgaattt aagtattgtc gttcctattt ataatgtcga aagttatttg gaagcgtgtt
     5101 taaattctat agaacctata ttaagtaatg aaaatgtcga acttattctt gtgaatgacg
     5161 ggtcaaaaga cggaagtgaa gatatatgtt ataaatatat agataaaata
//
LOCUS       DAXDSD010000115         5065 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXDSD010000115 DAXDSD010000000
VERSION     DAXDSD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.115"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            118..858
                     /gene="pyrF"
                     /locus_tag="ACFB2Q_001883"
     CDS             118..858
                     /gene="pyrF"
                     /locus_tag="ACFB2Q_001883"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC0918850.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
     gene            914..1876
                     /gene="hldA"
                     /locus_tag="ACFB2Q_001884"
     CDS             914..1876
                     /gene="hldA"
                     /locus_tag="ACFB2Q_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC0918851.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            1940..2944
                     /gene="rfaD"
                     /locus_tag="ACFB2Q_001885"
     CDS             1940..2944
                     /gene="rfaD"
                     /locus_tag="ACFB2Q_001885"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC0918852.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGCMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            3019..4563
                     /locus_tag="ACFB2Q_001886"
     CDS             3019..4563
                     /locus_tag="ACFB2Q_001886"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC0918853.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQGIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSIDWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTCIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREVIDIRQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            4679..>5065
                     /locus_tag="ACFB2Q_001887"
     CDS             4679..>5065
                     /locus_tag="ACFB2Q_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFC0918854.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGE"
BASE COUNT         1364 a         1470 c         1227 g         1004 t
ORIGIN      
        1 ccttagggaa ccgttccctt tgagccgggg cggggcaacg acgtaccggt ttttgttaat
       61 ccgctatact gcgaaaaaga cgataaaggt cgtctgaaaa cccgaaacga aaacaccatg
      121 aatcctttaa tcaccgactt ccaaactccg caacaacgta cccccgtcat cgttgccctt
      181 gattttgcca acgaaaaaga cacgctcgga ttcgtccgca accttgatcc ggcgttgtgc
      241 caaatcaaaa tcggcaaaga gctgtttacc gcgacgggac gcagtttggc ggaaagcctg
      301 attcatcagg gtttcaaact ctttcttgat ttgaaatacc acgatattcc ccacaccgtc
      361 gctcaggcgt gtaaagtcgc cgccgatatg ggcgtgtgga tggtcgatat gcacgcgtcg
      421 ggcggcagac gtatgatgga agccgccgcc gaagccgttg ccggatacgg cacgaaaccg
      481 ctcttaatcg gcgtaaccgt gttgaccagc atggagcaaa gcgatttggc ggaaatcggt
      541 ttgaacaccg cccctgaaga acaagtcatc cgtttggcaa aactggcaca aagttcgggc
      601 ttggacggcg tggtctgttc cgcccaagaa gccgcgccgc tgcgccgcga attgggacgg
      661 gattttgtct tggttacacc gggtatccgc ttggacgttg ccggcaacaa cgacgaccaa
      721 cgccgcatta tgacacccgc cgaagccttg gcggcgggtt cgacttatct ggtgatgggc
      781 cgccccgtta cccgagccgc cgatccggtc gccgtattgc gcgaagtgaa ccgcgtggca
      841 aaccttgaag caaactgatt ttcaggcggt cttacaggtt aaggttgtct gaacaaaaca
      901 caacggaggc aatatgcccg ccaagttcca acaagaaacc ctcaaatccc gtttcgcaca
      961 agccaaagtt cttgttgtcg gcgacgtgat gctcgaccgc tattggtttg gcgacgtgtc
     1021 ccgaatttcg cccgaagcac ccgtgccggt ggcaaaaatc ggacgaatcg accaacgggc
     1081 gggcggcgcg gcgaacgttg cgcgcaacat cgcttcgttg ggcggcaggg cagggctgtt
     1141 gtccgtaacc ggcgacgacg aagccgccgc cgcgctcgac gcgctgatgg tgcaggacgg
     1201 cgtcgcctcc tatctgatgc gcgacaaaca aatcgccacc accgtcaaac tgcgcgtcgt
     1261 cgcccgcaac cagcagctta tccgccttga ttttgaagaa catcccaacc gcgaagtgtt
     1321 ggaacaaatc aagcggaaat accgcgaaat cttgcccgaa tacgacgcaa tcattttttc
     1381 ggactacggc aaaggcggtc tgtcgcatat ctccgatatg atcgattggg cgaaacatgt
     1441 cggcaaaacc gtcttaatcg accccaaagg cgacgattac gaaaaatatg tcggcgcaac
     1501 tctgattacg cccaactgcg ccgaattgaa agaagtggtt ggcagttgga aaaacgaagg
     1561 cgatctgacc gaaaaagcgc aaaacctgcg ccgccacctc gacttgaccg ccgttttgct
     1621 gacccggagc gaagagggca tgaccttgtt cagcgaaggc gaacccattt accagcccac
     1681 ccgcgcccaa gaagtttacg acgtgtccgg cgcaggcgac accgtcattg ccggaatggg
     1741 cttggggctg gcggcaggct gcaccatgcc cgaagccatg taccttgcca atactgcggc
     1801 cggggttgtc gtggcgaaac tcggtacggc ggtttgctcg tttgcagagt tggttgaagc
     1861 actggacggg caataaatct tttcagacgg catgaccatc caatgccgtc tgaaaccctc
     1921 aaaacaaagg aaaccgaata tgaccatcat cgtaacaggc gcggccggct ttatcggcag
     1981 caacatcgtc aaagccctca accaacgcgg cattaccgac atcgtcgccg tcgataatct
     2041 gacaaaaggc gaaaaattca aaaaccttgc cgagtgcgaa atcgcccact acctcgacaa
     2101 acacgaattt atccgccaag tgagggaaca cattttacct tatcaaaaca tcgaagccgt
     2161 tttccatcaa ggcgcgtgtt ccgatacgat gaaccacgac ggtttgtata tgatggaaaa
     2221 caactaccag tacacgctgg atttgttgga ctggtgtcag gacgaacgca tccccttcct
     2281 ttatgcctcc agtgcggcgg tttacggcaa aggcgaaatc ttccgcgaag agcgcgaact
     2341 cgaaaaaccg cttaatgtgt acggctactc caaattcctg tttgaccaag tattgcgccg
     2401 ccgcatgaaa gaaggtctca ccgcccaagt cgtcggcttc cgctacttca acgtttacgg
     2461 acaacacgaa caacacaaag gctgcatggc atccgtcgcc ttccaccatt tccaccaata
     2521 ccgcgaacac ggttacgtca acctgttcgg cagcaacgac ggctacggca acggcgaaca
     2581 aacccgcgac ttcgtcagcg tcgaagacgt cgccaaaatc aacctctact tcttcgacca
     2641 tcccgaactt tccggcatct acaacctcgg caccggccgc agccaacagt tcaacgaact
     2701 cgccgccgcc accgtcaacg cctgccgcgc cgccgaaggc aaatctgaat tgagcttgaa
     2761 agagttggta gaagaagaac ttatccgcta tatccccttt cccgacgcgc tcaaaggcaa
     2821 ataccagggc ttcacccaag ccgacatcac caaattgcgc gaagccggat ataaggaaga
     2881 atttttcgat gtcaaagcag gtgtcaaccg ctacgtcaaa tggatgctgg aaaatttggc
     2941 ttaatttgaa tgctcgtaaa aaaatcgtct gaaaatatca ggcgattttg atttgtttaa
     3001 ctttatatat ggatttcgat gatgaccgaa atgcaacaac gcgcccaact gcaccgccaa
     3061 atttggaaaa tcgccgacga agtacgcggc gcggtggatg gctgggactt taaacaatac
     3121 gttctcggca cacttttcta ccgctttatc agcgaaaact tcaccgacta tatgcaggcc
     3181 ggcgacagca gcattgatta cgccgctatg ccggacagca tcatcacgcc cgaaatcaaa
     3241 gacgatgccg tcaaagtcaa aggctatttc atctaccccg gccagctttt ttgcaatatt
     3301 gccgccgaag cccatcaaaa cgaagagctc aacaccaagc tgaaagaaat ctttaccgcg
     3361 attgaaagct ccgcctccgg ctacccgtcc gaacaaggca tcaaaggctt gtttgacgac
     3421 ttcgacacca ccagcagccg gctcggcagc accgttgccg acaaaaacaa acgccttgcc
     3481 gccgtcctta aaggcgtggc ggaactcgat ttcggcaatt ttgaagacca ccgcatcgac
     3541 cttttcggtg atgcctacga atacctgatt tccaactacg ccgccaacgc aggcaaatcc
     3601 ggcggcgaat ttttcacccc gcaaagcgtc tccaagctga ttgcgcggct ggcggtgcac
     3661 gggcaggaga aagtcaacaa aatctacgac cccgcctgcg gctcgggcag cctgctcttg
     3721 caggcgaaaa aacagtttga cgaacacatc atcgaagaag gcttcttcgg gcaggaaatc
     3781 aaccacacca cctacaacct cgcccgcatg aatatgtttc tgcacaacgt caattacaac
     3841 aaattccaca tcgaattggg cgacacgctg accaacccca aactcaaaga cagcaaaccc
     3901 tttgatgccg tcgtctccaa tccgccctat tccatcgact ggataggcag cgacgacccc
     3961 accttgatca acgacgaccg ctttgccccc gcaggcgtac tcgcaccgaa atccaaagcc
     4021 gattttgcct tcatcctgca cgcactgaac tacctttccg gcagaggccg cgccgctatc
     4081 gtctcattcc ccggcatttt ctatcgcggc ggcgcagagc agaaaatccg ccaatatctg
     4141 gtggagggca actatgtgga aaccgtgatt gcccttgcgc ccaatctctt ttacggcacc
     4201 tgcatcgccg tcaatatcct ggttttgtcc aaacacaaag acaataccga catccaattc
     4261 atcgatgcag gcggcttctt taaaaaagaa accaacaaca acgtcttaac cgaagaacac
     4321 attgccgaaa tcgtcaaact cttcgccgac aaagccgatg tgccgcatat cgcccaaaac
     4381 gccgcccagc aaaccgtcaa agacaacggc tacaacctcg ccgtcagcag ctatgtcgaa
     4441 gccgaagaca cccgcgaggt catcgacatc agacagctta acgccgaaat cagcgaaacc
     4501 gtcgccaaaa tcgaacggct gcggcgtgaa attgacgaag tgattgcaga gattgaggcc
     4561 taggcagttg ttgcatattt tgcaacaact gaatgggaaa gaggccgtct gaaaatacgg
     4621 gcggcaacct gtaagcaatc cttacaagtt caaatcaaga aattttcggg gaaaatacat
     4681 gacaacagaa aataacgctt ttgaaaacgc caaacacatc gacgaaacag gcaatgaata
     4741 ttggtcggcg cgcaccttgc agcaaatctt ggaatattcc gaatggcgca atttccaacg
     4801 ggccattgat aaagccataa ccgcttgcga aacatcagga aatgacaaaa atcatcattt
     4861 tgttgaaacc aacaaaatga tagcccttgg gaaaggcggg cagcgcgaag tggcggatta
     4921 ccgcctttcc cgctatgcct gctacctgat tgtgcaaaac ggcgatccgt ccaaaagcgt
     4981 cattgccgca gggcaaacct actttgccgt gcaagcccgc cgccaagagc tgcaagacga
     5041 ggcagcattc aggagtttgg gcgaa
//
LOCUS       DAXDSD010000116         4964 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXDSD010000116 DAXDSD010000000
VERSION     DAXDSD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.116"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..429
                     /gene="rfaE2"
                     /locus_tag="ACFB2Q_001888"
     CDS             <1..429
                     /gene="rfaE2"
                     /locus_tag="ACFB2Q_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFC0918855.1"
                     /translation="LPRPLVFTNGCFDILHRGHVTYLAQARSAGAALVLALNTDASVR
                     RLGKGGDRPVNSLENRAAVAAALESVDLVTWFDEDTPAALIEAVKPEVLVKGGDWVVD
                     KIVGAAETLARGGQVFSIPFLHQTSTTKTLAKIRAAEGGK"
     gene            426..2204
                     /locus_tag="ACFB2Q_001889"
     CDS             426..2204
                     /locus_tag="ACFB2Q_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC0918856.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSIMMMHGRLKEKNGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            2317..3141
                     /locus_tag="ACFB2Q_001890"
     CDS             2317..3141
                     /locus_tag="ACFB2Q_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC0918857.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPVPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(3647..3859)
                     /locus_tag="ACFB2Q_001891"
     CDS             complement(3647..3859)
                     /locus_tag="ACFB2Q_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFC0918858.1"
                     /translation="MPSEGFRRHRGPKRANRHSPASRLCREEQGNRPENLAARPDTAT
                     NETPGLPGAQAAAQALSTAIEGSFVV"
     gene            complement(3874..4662)
                     /locus_tag="ACFB2Q_001892"
     CDS             complement(3874..4662)
                     /locus_tag="ACFB2Q_001892"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC0918859.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            complement(4741..4935)
                     /gene="thiS"
                     /locus_tag="ACFB2Q_001893"
     CDS             complement(4741..4935)
                     /gene="thiS"
                     /locus_tag="ACFB2Q_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFC0918860.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     TETVLHENDKIDIVRPVVGG"
BASE COUNT         1035 a         1302 c         1605 g         1022 t
ORIGIN      
        1 ttgccgcgcc cgctggtgtt taccaacggc tgtttcgaca tcctccacag ggggcacgtt
       61 acttatctgg cgcaggcgcg ttcggcaggg gcggcgttgg tgctggcgtt gaataccgat
      121 gcttcggtgc ggcgtttggg caagggcggc gaccgcccgg ttaattcttt ggagaaccgt
      181 gccgccgttg ccgccgcgtt ggaaagtgtg gatttggtta cttggtttga cgaggatacg
      241 ccggcggcgt tgattgaggc ggtcaagccg gaggtgttgg tcaagggcgg cgattgggtc
      301 gtggataaga ttgtcggtgc ggcggaaacg ctggcacgcg gcggtcaggt gttttcaatt
      361 ccgtttctgc atcagacttc gacaacgaag actttggcaa aaatccgtgc ggcagagggc
      421 ggaaaatgac ggttttgaag ccttcgcact ggcgggtgtt ggcggagctt gccgacggtt
      481 tgccgcaaca cgtatcgcaa ttggcgcgtg aggcggacat gaagccgcag cagctcaacg
      541 gtttttggca gcagatgccg gcgcatatac gcgggctgtt gcgccaacac gacggctatt
      601 ggcggctggt gcgccccttg gcggttttcg atgccgaagg tttgcgcgat ctgggggaaa
      661 ggtcgggttt tcagacggca ttgaagcacg agtgcgcgtc cagcaacgac gagatactgg
      721 aattggcgcg gattgcgccg gacaaggcgc acaaaaccat atgcgtgacc cacctgcaaa
      781 gtaagggcag ggggcggcag gggcggaagt ggtcgcaccg tttgggcgag tgcctgatgt
      841 tcagtttcgg ctgggcgttt gaccggccgc agtatgagtt gggttcgctg tcgcctgttg
      901 cggcacttgc gtgccggcgc gctttggggt gtttgggttt ggaaacgcaa atcaagtggc
      961 caaacgattt ggtcgtcgga cgcgacaaat tgggcggcat tctgattgaa acagtcaggg
     1021 cgggcggtaa aacggttgcc gtggtcggta tcggcatcaa tttcgtgctg cccaaggaag
     1081 tggaaaacgc cgcttccgtg cagtcgctgt ttcagacggc atcgcggcgg ggcaatgccg
     1141 atgccgccgt attgctggaa acattgcttg cggaactggg cgcggtgttg gaacaatatg
     1201 cggaagaagg gttcgcgcca tttttaaatg agtatgaaac ggccaaccgc gaccacggca
     1261 aggcggtatt gctgttgcgc gacggcgaaa ccgtgtgcga aggcacggtt aaaggcgtgg
     1321 acggacgagg cgttctgcac ttggaaacgg cagagggcga acagacggtc gtcagcggcg
     1381 aaatcagcct gcggcccgac gacagaccgg tttccgtgcc gaagcggcgg gattcggaac
     1441 gttttttgct gttggacggc gggaacagcc ggctcaagtg ggcgtgggtg gaaaacggca
     1501 cgttcgcaac cgtcggcagc gcgccgtacc gcgatttgtc gcctttgggc gcggagtggg
     1561 cggaaaaggc ggatggaaat gtccgcatcg tcggttgcgc cgtgtgcgga gaatccaaaa
     1621 aggcacaagt gaaggaacag ctcgcccgaa aaatcgagtg gctgccgtct tccgcacagg
     1681 ctttgggcat acgcaaccac taccgccacc ccgaagaaca cggttccgac cgttggttca
     1741 acgccttggg cagccgccgc ttcagccgca acgcctgcgt cgtcgtcagt tgcggcacgg
     1801 cggtaacggt tgacgcgctc accgatgacg gacattatct cggcggaacc atcatgcccg
     1861 gcttccacct gatgaaagaa tcgctcgccg tccgaaccgc caacctcaac cgccccgccg
     1921 gcaaacgtta ccctttcccg accacaacgg gcaacgccgt cgcaagcggc atgatggacg
     1981 cggtttgcgg ctcgataatg atgatgcacg gccgtttgaa agaaaaaaac ggcgcgggca
     2041 agcctgtcga tgtcatcatt accggcggcg gcgcggcgaa agtcgccgaa gccctgccgc
     2101 ctgcattttt ggcggaaaat accgtgcgcg tggcggacaa cctcgtcatc cacgggctgc
     2161 tgaacctgat tgccgccgaa ggcggggaat cggaacacgc ttaaaaaata tgcctttttt
     2221 caacacgggg cggcatccgt ccgaatctgt ccggattctg ccgcccgaca gactgccgcc
     2281 cgggcgggcg gggtttcaaa cattgaggaa attctaatga aatggctctt cacccttttg
     2341 gcggtactca atattgccgt attcggcggc acggtgggct ataaattggc cgttaaagcg
     2401 gcgggcggcg tgccggaaaa ccgggccgtc gaaaacacgc cccccgcaac gcccgccgcc
     2461 ggcaatgcgg cggcatccgt ggaggatacg gcggcactgc tcaaaccggg cgacatcctg
     2521 agcgaagaac aggcggagca gttgcgcttg aaaaaagaag cggaacagaa aaaactgagg
     2581 gagaaaaaac agcgtgaaga aaaagcccgc cgcgaaaaac tcgccgccga aaaggcgcag
     2641 gcggaacgtg aaaacggcgc ggcggatgcc ttatgcgccg cgcaggcaag cctcacgatg
     2701 gacgaagacg actaccaccg catcaaaggg cttttgggca aatggtcgca cgttgccagc
     2761 aggagcgtcg aaaaacgcac cgccaaagcc aaacctgccg acaaaaccta ccgcgtcgtc
     2821 ctgcccgttt ccgccgatgc cgaaaatcag gcggcggagc tgtctgccaa aggtttcaac
     2881 cccgtaccgt ttgacggcgc attgagtttg ggtgtcggca acagccggga aaacgcccaa
     2941 gccctgcaaa accgacttgc cgatgccgga ttcggcgggg cgcatattgt cgaacacttt
     3001 gccgaagccg acaggcagga cgattctttg agcgtgtcgc gtatgacggt tttgtttacg
     3061 ggcgtgaatg ccgccgatgc ggacgaaatc cgtaaaatca cgtccctata cggcaaactg
     3121 aacctcaagt cttgcaaata agtgcagacc aaaaacgcca aacccaccgc tagggtgggt
     3181 ttggcgtttc ggttttgagt atctgattaa aaagggaatg tatgtctcgt atatgccgac
     3241 tccaagtgtg aaagtgaatt gtgagggaat acagtgttta atggttcgga acggacggct
     3301 tccgagtggt tttgattata cgctaataat aataattatc aataaaaatt taaaaataaa
     3361 tgcggatttt ttgtaatgcg atgaaaaata aacaaattaa tctgtccgcc gatgtttgtg
     3421 atttgggtcg gcaaactttc attgcgccgg acaaatcggg gtcggcaggc atggacgggg
     3481 gcttcgtgcc tgtcggaaag aagcctgccg tgtttgaaca accgtccgat ttggtgcgga
     3541 cagggttgcc attcgggggt gtctgagcgc ggattcgggg atgcaaaagc ggtgaaaccg
     3601 ttgcggatga aggcttacgg atgccccgag gtatttttaa atgtattcaa acgacaaaac
     3661 tcccttctat tgccgtactt agggcttgcg cagcagcctg cgcgccgggg agtccgggtg
     3721 tttcgttggt tgcggtgtcg gggcgggcgg caaggttttc cggccggttt ccctgttcct
     3781 cacgacaaag ccgtgatgcc ggggaatggc ggtttgcccg tttggggccg cgatgccgtc
     3841 tgaagccttc agacggcatt tttgccgcct ttttcaatat tccgccgaat gccaaaacgg
     3901 ttgcccgact gtcggcgtgc tggcttgggc tttggttcgc gcttcgaccg gcccggcttc
     3961 aaatgccagc cgtccggatt cgacggcgag tgcgaaggcg cgcgccatgt tgacggggtc
     4021 gccgctgcgg gaaacggcgg tgtttaacaa tacgccgtca aaaccccatt ccatcacttg
     4081 tgccgcttgg gaaggcaaac ccaagcccgc gtcgataatc agcggcgtgt cgggcaggcg
     4141 ttcgcgcagg attttgagcg cataggcgtg aaccgccccc aaacccgtgc cgatgggagc
     4201 cgcccacggc atcaacgcct gacagcccgc atcgagcagg cggcggcagg caatcaggtc
     4261 ttcggtgcaa taaggcaaca ctttgaagcc gtctttaatc aggatttccg ccgcttcgac
     4321 gagttggaac acgtccggct gcaaggtgtc gtcgtcgccg atgagttcca attttatcca
     4381 atcggtttca aacacttcgc gcgccatttg cgccgtcgtt accgcttcct gcacgctttg
     4441 gcagcctgcc gtgttcggca ggacgggaac gccggtttct tgaagcagcg accaaaaccc
     4501 ctgaccgtgc gcctcgccgc cgcatcccgt gcggcgcagc gagacggtaa tcatcgcggg
     4561 ccgggcggtt cggacggatt gtttgaggat ttcaggggtc gggtaggcgg ctgtgccgag
     4621 cagcagccgc gaagggaaag tttcgccgta cagggtgagc atgatggatt ctttggtatg
     4681 gttgtcggac gcgatgccgt ctgaaagccg gcggcgtgcc tttggggttt cagacggcat
     4741 tcagccgccg acgacggggc ggacgatgtc gattttgtcg ttttcgtgta aaacggtttc
     4801 cgtatacgcg cctttgggga tgaaaacggt gttgaccgcc acggcaaagg gtttttgcgg
     4861 cgcggtttgg gcgatgaggt cggcaacgct tgtgccgtga agttcggcgg gtccgccgtt
     4921 taagatgatg ttcatggttt ctcgatgtct ctgtatttat agcg
//
LOCUS       DAXDSD010000117         4952 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXDSD010000117 DAXDSD010000000
VERSION     DAXDSD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.117"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            363..1007
                     /gene="rplC"
                     /locus_tag="ACFB2Q_001894"
     CDS             363..1007
                     /gene="rplC"
                     /locus_tag="ACFB2Q_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC0918861.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGVAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1007..1627
                     /gene="rplD"
                     /locus_tag="ACFB2Q_001895"
     CDS             1007..1627
                     /gene="rplD"
                     /locus_tag="ACFB2Q_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC0918862.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFVIEALTAETPKTKVFAEQVKNLALEQVLFVTKRLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1624..1938
                     /gene="rplW"
                     /locus_tag="ACFB2Q_001896"
     CDS             1624..1938
                     /gene="rplW"
                     /locus_tag="ACFB2Q_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC0918863.1"
                     /translation="MNQQRLTQVILAPVVSEKSNVLAEKCNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRILGRRSDVKKAYVSLADGQELDLEAAAAAAD
                     KE"
     gene            1944..2777
                     /gene="rplB"
                     /locus_tag="ACFB2Q_001897"
     CDS             1944..2777
                     /gene="rplB"
                     /locus_tag="ACFB2Q_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC0918864.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGISAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGVVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2783..3061
                     /gene="rpsS"
                     /locus_tag="ACFB2Q_001898"
     CDS             2783..3061
                     /gene="rpsS"
                     /locus_tag="ACFB2Q_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC0918865.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3070..3399
                     /gene="rplV"
                     /locus_tag="ACFB2Q_001899"
     CDS             3070..3399
                     /gene="rplV"
                     /locus_tag="ACFB2Q_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC0918866.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3409..4101
                     /gene="rpsC"
                     /locus_tag="ACFB2Q_001900"
     CDS             3409..4101
                     /gene="rpsC"
                     /locus_tag="ACFB2Q_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC0918867.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4085..4501
                     /gene="rplP"
                     /locus_tag="ACFB2Q_001901"
     CDS             4085..4501
                     /gene="rplP"
                     /locus_tag="ACFB2Q_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC0918868.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4501..4692
                     /gene="rpmC"
                     /locus_tag="ACFB2Q_001902"
     CDS             4501..4692
                     /gene="rpmC"
                     /locus_tag="ACFB2Q_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC0918869.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4692..>4952
                     /gene="rpsQ"
                     /locus_tag="ACFB2Q_001903"
     CDS             4692..>4952
                     /gene="rpsQ"
                     /locus_tag="ACFB2Q_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC0918870.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
BASE COUNT         1407 a         1062 c         1201 g         1282 t
ORIGIN      
        1 ttgtcctgat ttaaatttaa tccactatat tttaaacaac aaagaattga atttggaaga
       61 acggcatgat ttggagcggg cggttaagat cttgcaggac acgcacgtca tcccggtttg
      121 tcggagaaag atgcatacgc cgtgatggtc ggataccgac actttaaaag catccgaccc
      181 catgccttct gaagattcaa aacgcttcag acggcatatt gaagatattt ctgatatttc
      241 tgttgatatt tctttgactt gtcggatata atgtcaagct tggtacattt ttgccaagtt
      301 taactttatc tgaaagacag gccaatcgca gcctgtccct ttactttaaa aggaaaataa
      361 tcatgacttt aggtctggtt ggacgcaaag ttggtatgac ccgcgtgttc gacgagcagg
      421 gtgtttctgt tccggtaacc gttttggata tgtctgccaa ccgcgttaca caagtaaaat
      481 ccaaagatac tgacggctat accgccgttc aagttacctt tggtcagaaa aaagccaatc
      541 gtgtcaacaa agccgaagcc ggacactttg caaaagcagg tgttgaagcc ggtcgcggtt
      601 tgattgagtt tgctttgact gaagaaaaac tggctgaatt gaaagccggt gacgaaatca
      661 ccgtttctat gtttgaagtc ggtcaactgg tcgatgtaac cggtacctct aaaggtaaag
      721 gtttctccgg cacgatcaaa cgtcataact tcggtgccca acgtacttcc cacggtaact
      781 cccgttctca ccgcgttcca ggctctatcg gtgtggcgca agacccgggt cgcgtgttcc
      841 ccggtaagcg catggccggc caatacggca acaccaaagc aactgttcaa aaattggaag
      901 ttgtccgtgt tgatgcagaa cgccaactgc tgttggttaa gggtgctgtt ccgggtgcgg
      961 tcaacagcga tgttgtagtt cgtcccagcg tgaaagtagg tgcgtaatgg aattgaaagt
     1021 aattgacgct aaaggacaag tttcaggcag cctgtctgtt tctgatgctt tgttcgcccg
     1081 cgaatacaat gaagcgttgg ttcaccagct ggtaaatgcc tacttggcaa acgcccgctc
     1141 tggtaaccgt gctcaaaaaa cccgtgccga agtaaaacac tcaaccaaaa aaccatggcg
     1201 tcaaaaaggt accggccgcg cccgttccgg tatgacttct tctccgctgt ggcgtaaagg
     1261 cggtcgcgcg ttcccgaaca aacccgacga aaacttcact caaaaagtaa accgtaaaat
     1321 gtaccgtgcc ggtatggcga ctatcctgtc ccaattggcg cgtgacgagc gtttgtttgt
     1381 gattgaggcg ttgactgccg aaactcccaa aaccaaagtt tttgccgaac aagtaaaaaa
     1441 tttggctctg gagcaagtgc tgtttgtaac caaacggctc gacgagaatg tttacttggc
     1501 ttcacgcaac ttgccaaacg tattggtttt ggaagctcaa caagttgatc cttacagctt
     1561 gctgcgttat aaaaaagtaa tcatcactaa agatgcggtt gcacaattag aggagcaatg
     1621 ggtatgaatc aacaacgttt gactcaagta atcttggcgc ctgtcgtttc tgaaaaaagc
     1681 aacgtattgg ctgaaaaatg caaccaaatg acgtttaaag tcttggcaaa tgcaaccaaa
     1741 cctgaaatca aagctgctgt cgagctgctg ttcggtgttc aagttgcttc tgtaactacc
     1801 gttaccacta aaggcaaaac taagcgtttt ggtcgtattt tgggtcgccg cagcgatgtt
     1861 aaaaaagctt atgtaagctt ggctgacggt caagagttgg atttggaagc cgctgctgca
     1921 gctgcagata aggaataaac aaaatggcaa tcgttaaaat gaagccgacc tctgcaggcc
     1981 gtcgcggcat ggttcgcgtg gtaacagaag gtttgcacaa aggtgcacct tatgcaccct
     2041 tgcttgaaaa gaaaaattct actgccggcc gtaacaataa tggtcatatc accacccgtc
     2101 acaaaggcgg cggtcataaa caccattacc gtgttgtaga ctttaaacgt aacaaagacg
     2161 gcatttctgc taaagtagag cgtatcgaat acgatcctaa ccgtactgcc ttcatcgcac
     2221 tgttgtgcta tgcagacggc gagcgtcgtt acatcatcgc tcctcgcggt attcaagccg
     2281 gtgtcgtatt ggtttccggt gctgaagctg ccatcaaagt aggcaacacc ctgccgatcc
     2341 gcaacattcc cgttggtacg actatccact gtatcgaaat gaaacccggt aaaggtgctc
     2401 aaatcgcacg ttctgccggt gcttctgcgg tattgttggc taaagaaggt gcatacgctc
     2461 aagtccgtct gcgctctggc gaagttcgta aaatcaacgt agattgccgt gcgaccatcg
     2521 gtgaagtcgg taacgaagag caaagtctga aaaaaatcgg taaagccggt gctaaccgtt
     2581 ggcgcggtat tcgtccgacc gtacgcggtg ttgtcatgaa tcccgtcgat cacccgcatg
     2641 gtggtggtga aggccgtacc ggcgaagccc gcgaaccggt tagtccatgg ggtactcctg
     2701 ctaaaggcta ccgcactcgt aataacaaac gcacggataa tatgattgtt cgtcgccgtt
     2761 actcaaataa aggttaatta gtatggctcg ttcattgaaa aaaggcccat atgtagacct
     2821 gcatttgctg aaaaaagtag atgctgttcg cgcaagcaac gacaaacgcc cgattaaaac
     2881 ctggtctcgt cgttctacca ttctgcctga ttttatcggt ctgaccattg ccgtgcacaa
     2941 cggtcgcacc catgtgcctg tgtttatcag cgacaatatg gttggtcata aattaggcga
     3001 attctcattg acccgtacct ttaaaggcca cctggccgat aaaaaggcta aaaagaaata
     3061 aggtgaatca tgagagtaaa tgcacaacat aaaaatgccc gtatctctgc tcaaaaggct
     3121 cgtttggtag ctgatttgat tcgtggtaaa gacgttgccc aagctttgaa tattttggct
     3181 ttcagcccta aaaaaggtgc cgagctgatc aaaaaagtat tggagtcagc tattgctaat
     3241 gctgagcata ataacggtgc ggacattgat gaactgaaag tggtaactat ctttgttgac
     3301 aaaggtccaa gcttgaaacg ttttcaagct cgcgccaaag gtcgcggtaa ccgcatcgaa
     3361 aaacaaactt gtcatatcaa tgtgacagtg ggtaactaag gaaaagctat gggacaaaag
     3421 attaacccta caggctttcg cctggcggta actaaagact gggcttcaaa atggtttgct
     3481 aaaagtaccg acttttctac tgttttgaag caggatatcg atgttcgcaa ttatttgcgt
     3541 caaaaattgg ccaatgcttc ggttggtcga gtaattattg aacgccctgc aaaatctgca
     3601 cgcattacca ttcactccgc tcgtccgggt gtggttatcg gtaaaaaagg tgaggatatc
     3661 gaagttttga aacgtgactt gcaagtcttg atgggtgtac ctattcatgt aaatattgaa
     3721 gagattcgcc gtcctgagtt ggatgctcaa attattgctg acggtatcgc ccagcagttg
     3781 gaaaaacgcg ttcaattccg tcgtgctatg aaacgagcaa tgcaaaatgc aatgcgttct
     3841 ggtgctaaag gcattaagat tatgacttca ggccgtctga atggtgcgga tattgcccgt
     3901 agcgaatggt atcgtgaagg tcgcgtgcca ctgcatactt tacgtgcaaa tgtagattat
     3961 gcaaccagcg aagcgcacac cacatatggt gtattgggtc tgaaagtttg ggtttatacg
     4021 gaaggcaata ttaaatcttc caaacctgaa catgagagta aacaaagaaa ggcaggtaga
     4081 cgtaatgctg cagccaacta gactgaaata ccgtaagcaa caaaagggtc gcaataccgg
     4141 catcgctact cgcggtaata aggtaagttt cggtgagttc ggcttgaaag ccgtaggtcg
     4201 tggtcgtttg actgcccgtc aaatcgaagc tgctcgtcgt gcaatgaccc gtcacatcaa
     4261 acgtggtggt cgtatttgga ttcgtgtatt ccctgacaaa ccgattactg aaaagcctat
     4321 tcaagttcgt atgggtggcg gtaaaggtaa cgtggaatat tacattgccg aaattaaacc
     4381 aggtaaagtg ttgtatgaaa tggatggcgt tccagaggaa ctggctcgtg aagcattcga
     4441 gttggctgct gccaaattgc ctattcctac aacctttgta gtaagacagg tgggtcaata
     4501 atgaaagcaa atgaattgaa agacaaatcc gttgagcagt tgaatgcaga tttgttggac
     4561 ttgttgaaag ctcagtttgg cttacgtatg caaaacgcta ccggtcaatt aggcaaacca
     4621 agtgaattga aacgtgtacg tcgcgatatt gctcgtatta aaaccgtttt aactgaaaaa
     4681 ggtgctaagt aatgagcgaa actaaaaatg ttcgtacttt gcaaggcaaa gtagtaagcg
     4741 acaaaatgga taaaaccgta acagtattgg ttgagcgtaa agtaaaacat tcgctgtatg
     4801 gtaagattat tcgattatct actaaaatcc atgcccatga tgaaaataat caatatggaa
     4861 ttggtgatgt ggttgttata tcggaatccc gtccattgtc aaaaactaaa tcttgggttg
     4921 tcagtgagct ggttgagaaa gcacgttcta tt
//
LOCUS       DAXDSD010000118         4705 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXDSD010000118 DAXDSD010000000
VERSION     DAXDSD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.118"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1350
                     /gene="yegQ"
                     /locus_tag="ACFB2Q_001904"
     CDS             <1..1350
                     /gene="yegQ"
                     /locus_tag="ACFB2Q_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC0918871.1"
                     /translation="APELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFAKL
                     DVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLIMT
                     VREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELGMEEIAEIRQECPDIEL
                     EVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLLQG
                     FNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNSKD
                     LRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLSEL
                     EGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNRFA
                     VGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLNP"
     gene            1481..1954
                     /locus_tag="ACFB2Q_001905"
     CDS             1481..1954
                     /locus_tag="ACFB2Q_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC0918872.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGIGKSDSEACEHAL
                     INAARKFQHTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRAVMRANVVR"
     gene            complement(2163..4536)
                     /locus_tag="ACFB2Q_001906"
                     /pseudo
     CDS             complement(2163..4536)
                     /locus_tag="ACFB2Q_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
BASE COUNT         1175 a         1302 c         1137 g         1091 t
ORIGIN      
        1 gcacccgaac tcttattgcc cgccggcgga ttggaaagaa tgcgcgccgc ctacgattac
       61 ggcgcagatg ccgtttacgc cggcagcccg cgttactccc tgcgcgcccg caacaacgaa
      121 tttgccaaac tcgacgtcct cgagcaaggc attaaagaag cgcacgaacg caacaaaaaa
      181 ttctttttga ccgtcaacac cctgccgcac aattccaaac tcaaaacctt cgttgccgac
      241 atggagccgc tgattgccat gaaacccgac gcgctgatta tggcggatcc gggtttgatt
      301 atgaccgtgc gcgaaaaatg gccggaaatg ccgatccatc tgtccgtaca ggcgaacacc
      361 accaactact ggggcgtgaa attttggcag aacattggtg tcgaacgcat catcctgtca
      421 cgcgaattgg gcatggaaga aattgccgaa atccgccaag aatgccccga catcgaactc
      481 gaagtcttca tccacggcgc attgtgcatc gcctactcag gccgctgcct gttgtcgggc
      541 tatttcaacc accgcgatcc caaccaaggc acctgcacca actcctgccg ttgggactac
      601 aaagtccaca acgccacgga aagcgaggca ggcgatgccc agcttctgca aggtttcaac
      661 tttgaaaaag cccaagaaga agccaaccaa aactttgaag gcatcaacgg tcaaaaacgc
      721 catccctacg ccgacaaagt tttcctgatt gaagaatcca accgcccggg cgagatgatg
      781 ccgattatgg aagacgaaca cggcacctac atcatgaact ccaaagatct tcgcggtatc
      841 gaagtcgtcg aaaaactcgc caaaatcggt gtggacagcc tcaaagtcga aggccgtacc
      901 aagtctctct actacgtcgc ccgcgtcgcc cagtcctacc gcaaagcgat tcacgatgcc
      961 gtcgcaggcc gtccgtttga ttacagcctg ttgagcgaac tcgaaggcct tgccaaccga
     1021 ggctacacca gcggcttcct cgaacgccac caaactcagg attatcaaaa ctacctcagc
     1081 ggccattcca ccgccaaaca aagccaatac gtcggacacg ttaccgaaat cgacgaaaat
     1141 ggctgggcga ccatcgaagt taaaaaccgc ttcgccgtcg gcgattcgct cgaaatcatc
     1201 cacccgagcg gcaaccaaac catcaaattg gaacaaatga cccgcaaagg ccaacctgtc
     1261 gatgttgccc cgggcaacgg cattcaggtc aaaatcccca atatgcaggg caaagaaaaa
     1321 gccctcatcg cacgcgtgtt gaacccctga gccacaatgc cgtccgaacc ccgttttcag
     1381 acggcatttt gttgaatctt actgtacaat ggcaatgatt tcccacagca aacaaggaca
     1441 tccgacataa aaaatcctca tactctccgc cgccgccctg atgctggcag cctgccaatc
     1501 cggccgcgcg cttaaaaacg cgcacacggc atccgccggg acaccggccg gagaatcatg
     1561 caaagttatc cgcggagaca ccgcaggagg cggcaaaaac atcatttacc gttgcagcaa
     1621 cggtcaggcg gcaataaatg cagccaccgc cgccggcgta ttggaatcag ggattcccgt
     1681 cagcttcggc ggcggcgtac cggttgcaaa cggcaggaat ctagttacac ggcaagcggc
     1741 aaacggcatt ggcaaatccg actccgaggc ctgcgaacac gccctaatca atgccgcccg
     1801 caaattccag cataccgccg gcaaactcgg cgggcgcagt gttaccggct tccacagcta
     1861 ctttggcaag caatcgctgc aaggcggaca atacgactgt caggcaggtt cgttccacgt
     1921 ccgcgcggtc atgcgcgcca atgtcgtccg ttaacatatc ggcaatcaaa aaatgccgtc
     1981 tgaaacattt ttcagacggc atttttaatc ctgcaacatt actccctgcc ctgagttcgg
     2041 atactgtatc aatataaaac cccatcacac agatttacgg taaaaagccg tccgaatgag
     2101 ttcctgaaaa caattcggac agctttaatc cccaacgagg caattaatcc aataatacta
     2161 gattaaaact tccattccag cgatacggcg taattacggc ctgaggcgcg gtagcggtct
     2221 aagcctttgc catctcggtc gaccgcgttg gtggtgctgt agctatacaa accgcgcagg
     2281 gaatcccaag tggtgtattt gcggttgaac acattatata cgcctgcacg caaagtcagg
     2341 tttttagccg gtttgtagaa gccgtacata tcaaacacat aagccgactt gttcagccac
     2401 gggtaatctt ttaccttttt ctgcaaaggc gtaccccggc ccttgttttc ataaacggtg
     2461 tattgcgcgt ctttggcctt tttcgcaccc agataagtca ggcgggagaa cacaccccat
     2521 ttttcgctcg ggcttcgtag tcgacaccgg caatcacttt cggcggctgt gtggacagca
     2581 ggctgttgtc gcccgacagt ttgcttttcg cataacccag cgagccgaac aatttccagc
     2641 cctcaggaac aaaagacgct attttgtcca cattcagacg gcctgtcagc tcaagaccac
     2701 ggattcgggc cttatcgata ttttgcatct gccattccgg tttttcggaa taaggattgc
     2761 tacacatacc gtagtagtaa ttcatctgag tacagccgac atcgccgctg gtggtcagct
     2821 tctgctcttc agacaagaag ttgcggtaat tgttttgata caggttggca tccaaagtac
     2881 ctttttcgct gcggccttgc agagacaggg tgtgggtggt gctgcgctcg gctttcaggt
     2941 tgggattggg cagccaatta cccgaaccgt ggttgtaagt gaaatacact tcggacgcat
     3001 tggggacacg gtagccggaa gtaatgtcgt aaccgacatg ccaagcctga ttcagttgcg
     3061 ccgccaaacc gacaaatccg ctccagcctt tataagtatt ggctgcaggc ggtgttttgt
     3121 cacaagcatg acactcggca ttcaattcct gaggcgtcat tttggtatga tcgtaacgga
     3181 tatctgcacg gctgctgaac acgtcgttcc attggatttg atcagacagt gagaaaccat
     3241 aattagtggt tttcacaggg tgttgaatcg agctggtagt acgggatatc tgtccgctga
     3301 agtaataatc gtcgcggttt aagttttcaa actcacgccg actggcgaaa gttttaaacg
     3361 acaagcgatg ttggccgccc agttgcaacg gttggctgtc catacgcaaa gtaaaacgtt
     3421 tgaatcgggt gtccatgctg cggttgtata tttcgtccaa atccttctga tttatagttg
     3481 cgcgtccagg tggaataatc catcgggaac gagcctttgt tgttaaccgc cgccactttg
     3541 gttttctgat aatcgaagtc cgccttcaaa gacgacaacc aatttgaatc aggcgtccat
     3601 tcgtaaaaga ggttggcatt gcgccgtctg tttacgtcat cggcttcgcg ccaggaagaa
     3661 gcggtcaggt tataagactc ttcaaatcgt gtaattatgc ccctgctggc cgttaaacga
     3721 tgcgccgatg cggtgcttgt cgttgatttg ataagcaatc ttacccaaga agttgtggta
     3781 tttgtgtttg gacggatcag ggataccgcg tgccgaacca cggatattcg ctccgctgcc
     3841 agcaccctct accggatagc cacgctcgcc cgcgctttcg gtctcatgac cgcgacgttg
     3901 cgaatacagc aaagcggcat ccacgcggtc gttgctcaca ccgaaaccga gtgtatttgt
     3961 ccattcgcgg ttgcggctgc tgtaaccgtt tttcatcatc acgccgaatt gcctgtcgtc
     4021 caacagcaaa tcatggcctt gcagggtttg gtaattcacg ccgccaccca atgcgccgct
     4081 accggtattg aaagagtcag cgcccttcgc gatttcgatg ttgcgcacga gttcggggtc
     4141 gatagacagg cgcgagctgt tgaagttgcc ataacgtgca tacagtgagt tttcttccga
     4201 atcaggcagg ctcacgccgt caatgctgac accgacacgg ttgccttcca cgccgcgcac
     4261 agcaaagcct ttttgatggc ggccgctatc gctcaagccg acgtcggtgg agtaacgcac
     4321 caagtctttg ttgtcgcgta tcatttcctg ttgaatgcgg ccgaggttga cacgttccac
     4381 ggttgcaggc gcattaagct ggtctttaac gcgcacttct tttatctctg ctttaacggg
     4441 tgtggtttcg gttgcagctt catccgctgc caagaccgga ttgccgaaaa tactgccgac
     4501 cagcgcggca ataggaagca tgtgtaatgg tttcatatta tcaactcaag atgtaatgga
     4561 ttgttcatcc atcagttaat tcataataaa ttttctgata atcattaata tttaataaga
     4621 cagcaacccg tacaaataaa tctgtgtcgt gtaattaaag gtctctgcaa acatctatgc
     4681 cgaggccttg tttgtttcat tattt
//
LOCUS       DAXDSD010000119         4677 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXDSD010000119 DAXDSD010000000
VERSION     DAXDSD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.119"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(189..611)
                     /locus_tag="ACFB2Q_001907"
     CDS             complement(189..611)
                     /locus_tag="ACFB2Q_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFC0918873.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
     gene            complement(617..1213)
                     /locus_tag="ACFB2Q_001908"
     CDS             complement(617..1213)
                     /locus_tag="ACFB2Q_001908"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HFC0918874.1"
                     /translation="MKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAEV
                     ISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTGL
                     HSIDGRELIEKAFGSKKSMLLLTNNKTQAEQNLEDGLEQLACGTWTGFRNPVQHELRA
                     NLSPSIFNDKDALDLISLVSYLLRKVEQTKKRAKPTSP"
     gene            complement(1403..2260)
                     /locus_tag="ACFB2Q_001909"
     CDS             complement(1403..2260)
                     /locus_tag="ACFB2Q_001909"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC0918875.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFRLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVKPNTENGTTIT
                     LTELTKKQGYPLDNYVGHLSRLFDFPAQDFKIKVSLNGSEPRIIDGNLKYNLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHF"
     gene            complement(2274..2603)
                     /locus_tag="ACFB2Q_001910"
     CDS             complement(2274..2603)
                     /locus_tag="ACFB2Q_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC0918876.1"
                     /translation="MAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPAPTITTKFFSI
                     SNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVPPMYAEKIGRA
                     IVDNIEC"
     gene            complement(2642..3250)
                     /locus_tag="ACFB2Q_001911"
     CDS             complement(2642..3250)
                     /locus_tag="ACFB2Q_001911"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC0918877.1"
                     /translation="MQVLAGIDYELSCKETYEANINAKFIQADIFELQPETLEKELGL
                     KKNDDDLILIGCSPCQYWSVIQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGI
                     LSRMKESGLDNFIKLLEEKGFTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSS
                     IRANGLRYAMFWEWKTAFPKLWQDIKTRRILCIVVRDYPISI"
     gene            3619..3765
                     /locus_tag="ACFB2Q_001912"
     CDS             3619..3765
                     /locus_tag="ACFB2Q_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918878.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            complement(4142..4591)
                     /locus_tag="ACFB2Q_001913"
     CDS             complement(4142..4591)
                     /locus_tag="ACFB2Q_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC0918879.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         1329 a         1059 c          741 g         1548 t
ORIGIN      
        1 tccgtcatca cgccggcaac ctcctttgtc atcccttgct tatcttccat ttgcgaatcc
       61 tcaaaaacgg gcaaaaaaaa agccctgtta cttgtagaaa gtaaagggcg ttaatttttg
      121 ttaatcaccc cttcttaggg acgcaatata taagggtctt aattaaagga agatgccgtc
      181 tgattttttc agacggcatc ttttatttcc tcgaccaacc gatttaatcg ctccctacaa
      241 atagctttat tttttaattc gcactcccaa acgacaatca ctttgaaacc aatcttttcc
      301 agctcggttt catttttaat atcacgttcg cgattttttg tgattttttc caaccaaaaa
      361 tccatattgc ttttcgggat atgtccttta ttacaagaat gcccgtgcca aaaacagccg
      421 tgtataaaca cgactgtttt atatttaggc aaaacgatat ccggtttgcc tgcatagcgc
      481 ttatcgtttt tccgataacg gaaaccttgg gaaaaaagaa atttccttac caatacttcc
      541 ggtttggttt ctttgctatg gatttttgac atcacgaagg aacgtttgga tggagtgaaa
      601 atatcggtca taaaatttaa ggcgatgtag gtttagctcg tttttttgtt tgttccactt
      661 ttctcaataa atatgaaact aagctgatta aatccaaagc atccttgtca ttaaaaatac
      721 taggagataa atttgctctt aactcatgtt gcacaggatt tctaaatcca gtccatgttc
      781 cgcatgcaag ctgttcaaga ccatcttcta aattttgttc cgcctgcgtt ttgttattcg
      841 ttaatagaag catagatttt ttactcccga aggctttttc aatcaattct cttccgtcta
      901 tgctatgaag acctgtctgc ttctgaacct tatcttcgaa aactttaact gcctcgattg
      961 cagcacttaa ataatccttc ttttcattgt aaaaatcgtt acaggcagta tgcaaatcct
     1021 gatgcaaatg ccgccaatga tagtaaggat attctggaat aatactatgt aaggcagaga
     1081 ttacttccgc ttgttcttta tttgtaagct ccgaatcttc caaacgattc aaaataactt
     1141 ctaccggttc tctttccttt tctggcaatt tgtttttcca attttcgata tcaactccgc
     1201 tttcaccttt cacttcgtaa tctttctctt tttttcttct ttttctccaa tcttgcccga
     1261 tggctcagac gatttcccca aggaaaaccc gtaattttga agttttagga tgcttccaat
     1321 ccaatgatgt cctatctgta gcgattaaat cttcaccatc attatctata aaatcaatac
     1381 ttaaccatcc tgtcagatag gattagaaat ggctggattc gctatcagtg aaaaactcgg
     1441 gcatatttac cattctgccg ttggcaaaca aggtaatacc tttcatatta ttctttaaag
     1501 gtttttccgt tgtaatgaac ttaccttgta ttaatccgct gtattcatac tgttcgaatt
     1561 ttgaagataa cgatgaaata ttggttgcta gatcctggta ttcccattcg aattgtgggg
     1621 taacaagatt atattttagg tttccgtcaa tgattcttgg ttccgagccg ttcaagctta
     1681 ctttgatttt aaaatcctga gccggaaaat caaataaacg ggaaagatgc cccacataat
     1741 tatctaacgg gtatccttgt tttttcgtca gctcggttaa agttatagtt gttccgtttt
     1801 cggtattggg tttaacagac tctttctgaa actccggttg ataaatacgc tcactttttt
     1861 taatctctgc ataatccaaa gtaaaagtaa cccgttcgtt tccttggata gtagagattt
     1921 cgattttgtt gccaagcctg aataatgcca atttaccaag acctttttta cccgttggaa
     1981 ttcttccgca cggggaggct tgtttttctt cccttctgtt ccgaccgatt ctcaaataaa
     2041 aatcattgat ttcatcgaag ctcattccta tgccattatc tttaataacg attttatgct
     2101 ccggtttatc gaataaccta acttccactt ccgtagcaca tgcatcgtag gcattcgcta
     2161 tcaactcggc aatcgcagga acagtgtgcg aatacatctt tacacccaaa tgttcgatta
     2221 cggttggctc gaaactcatt ttcaattttt cttcagacat gttttctcct tttctaacat
     2281 tcgatattat caacaattgc cctgcctatt ttttccgcat acatcggagg aacggcatta
     2341 ccaatcaaac gggctatctt gtccctactg cccgctttaa agacatagtt acgagggaac
     2401 gattgcaatg ttgcaccttc tcgcagggat aacgccctat cttcttcagg atgggcaaac
     2461 cgcccgttgg aaatgctaaa gaatttggtc gttatggtag gtgcgggctt atcccaccat
     2521 agcctaccga acgtgtcttt aaaactattg tcttttccga taaaacattc cagctgtaat
     2581 tcgggaatat gcgcaaaagc caaacggttt cctccgtttt taggtatcaa agccaatcgt
     2641 ttcaaattga tatcggataa tcccgcacaa ctatgcataa aatccgtctc gtcttgatgt
     2701 cctgccataa ttttgggaaa gccgttttcc attcccaaaa catcgcgtac cgtaagccgt
     2761 ttgcccgaat acttgactgg ttccagcttt ttttggttat tctgtttgca attaacgtaa
     2821 atcttttacg gctttgggga attccgtaat cagccgtatt gtgaataccg aaatggacgg
     2881 taaatccttt ttcttccaat aactttatga aattatcaag cccgctttct ttcattctgc
     2941 tcagaattcc cggtacgttc tctacaacaa catatccggg attgaaatat tctacaaacc
     3001 gctgaaactc caaaagcaaa ctcttggatt tttccgattt tcttttgtct gtttgaataa
     3061 cactccaata ttgacaagga ctacaaccta taagaatcaa atcatcatca ttctttttta
     3121 agccgagttc tttttccaag gtttcaggct gcaattcaaa aatatcagcc tgtataaatt
     3181 tggcatttat attggcttca tatgtttctt tgcagctaag ttcgtaatct atcccggcta
     3241 aaacctgaat accggcggat tacattccat agctcattcc gcctccgcca cagaaaaaat
     3301 caaccgcctt taacttactg tttttcattt ctatttaaat aattagacta gtgagaacta
     3361 actttatata acatataatt tattgcttat caaaaataat ttgagatttt attaaaattc
     3421 atgcatctaa ataagcagaa cttaatttcc tccaaacgat cggatatctt caaatgcaca
     3481 tttcacagaa aggtcgtctg aaacccattt tcagacgacc tttttccact tcgcaaaaca
     3541 agaattccca cccctctccc caaacatcgc ccaaaccagt aaattcccca tatttcaaca
     3601 actccttgga agcaaaccat gtcccgtatc tacctacccc tcctatttcc gccccacatc
     3661 gtcgaacgcg gccttttgta ttttcagcag gacaaggttc gcgatgtcca aaaaatttcc
     3721 ttagggcgtt atcgggcgaa ggtattcgat tcggaaaact attaggcatg gttgaagctg
     3781 gatagtgatt tgtacattaa ggacggggct gcgattgccc ttatgtctaa aggtgcaaac
     3841 atgccttaaa ttactaagcc tataacgaat tggaaacaaa atgccgtctg aaaacatctt
     3901 cagacggcat tataaaatct gttcaccttt tcagatgagt aatgtacacc cctatacaat
     3961 ttttgctacc atgcaccata aatccacggc taaagataat tgggtttatt cttttatcca
     4021 gaaacaatcc agatacggat cacgggtcat aactataggc ttaatattac acgattctca
     4081 ttccatcaag gcggaaaacc gcacaaatac tgaaacacta tcgatcgatt tgtaaacaag
     4141 cctacttaag taacttgcag tccttatcat ttcctttaaa ataatccagc ccgtcactac
     4201 acgaactggc ggacttcttg caaataaagg ttactagatt ttcattcatc ttaataataa
     4261 aaggattttt atctttatct atggctaccg ccttcaacat gaatttactg tctaaagccc
     4321 cgcgcgcgat tccattcaaa cggatacaaa agccttctgc ctctttaatc ggcaaacttg
     4381 gccatttggt agatgtttgt ttaaatctcc cattctgcag ataaaacttt tccataaaat
     4441 gtgcattttc taacaaggct gcccgcactg catttatctt tgctttctca acataattgc
     4501 gatagctcgg ataaacaatt aaagcaagta cagacaatat caagaccact gatattaatt
     4561 caaccagcgt aaacccccga ttatcagtca ttactttact tccaataaga acagattatt
     4621 caacatattt ctttgaacag acttactatc ccattcagca gtatgcatat ttcccac
//
LOCUS       DAXDSD010000120         4596 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXDSD010000120 DAXDSD010000000
VERSION     DAXDSD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.120"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            186..668
                     /locus_tag="ACFB2Q_001914"
     CDS             186..668
                     /locus_tag="ACFB2Q_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC0918880.1"
                     /translation="MMKKILAVSALCLMTAAAQAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVSLAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCMSSSYYGGAVRSLIQHLK
                     "
     gene            complement(715..1926)
                     /locus_tag="ACFB2Q_001915"
     CDS             complement(715..1926)
                     /locus_tag="ACFB2Q_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC0918881.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            2148..4586
                     /locus_tag="ACFB2Q_001916"
     CDS             2148..4586
                     /locus_tag="ACFB2Q_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC0918882.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     LNGSRKILAHKDHL"
BASE COUNT         1161 a         1257 c         1242 g          936 t
ORIGIN      
        1 ccgcaatgcc gtctgaaaac aacggcttgc cgctaaggcg gcaatatgag gatttgcgct
       61 gccgaaaccg gtttgctaca acccggcagt gaaatctgca gggagggata ggccgataag
      121 gcgggactta tcttgttttt tgtttataat gcaacagaat ttggaggaat cgtcgggaat
      181 ttatcatgat gaaaaagata ctggcagtat cggcactatg cctgatgact gcggcggcac
      241 aggctgccga tacttacggc tatctcgccg tttggcagaa tccgcaggat gcaaacgatg
      301 ttttgcaggt taaaaccaca aaagaagatt cggcgaaaag cgaagcgttt gccgagttgg
      361 aagccttttg caaaggtcag gacacgcttg cgggcattgc cgaagacgag ccgaccggat
      421 gccggtcggt cgtgtcgctg aacaatacct gtgtctcgct ggcatacccg aaagccttgg
      481 gcgtgatgcg cgttgaaaac gccgtcgtga ttacttctcc gcgttttacg agcgttcatc
      541 aggtcgcact caaccagtgc ataaaaaaat acggcgcaca gggacaatgc ggcttggaaa
      601 cagtgtattg catgtcatct tcttattacg gcggggctgt tcgctcctta atccaacacc
      661 tgaaataaaa aatgccgtct gaaggatgtt cggacggcat ttccatatcg acggtcagga
      721 ttctttcgga tcgggcagca ggctgttcaa cacaatggcg agtacggcgc acaagcccac
      781 gccgacaaag ctgaagctgc ccaatttgag tgtcataccg ccgatgcccg tggtcagtac
      841 cgagctgacg atgaccaggt ttttcggcag catcaaatcg actttggcat caatcagggt
      901 tttgatgcct aaagaagcaa tcgtgccgaa cagcagcagc ataatgccgc ccatcacggg
      961 catcggaatg gaagccaaaa acgcattgaa tttgccgaaa aacgccatgc agacggcaaa
     1021 aaccgccgcc caagtcatga tgacggggtt gctgtttttg gtaatcatca ccgcacccgt
     1081 tacttcgccg taggttgtaa ccggcgggcc gccgatcaga cccgcaacgc acacgcccaa
     1141 gccgtcgccc gcaagggttt tgtccaagcc cgggtctttt gtgtagtctt ttcccgttac
     1201 attgccgatt gccatgatgc cgccgatgtg ttcgatggcg ggggcgacgg caacgggcag
     1261 cataaacagt gccgcctgcc agttgacctg aggcgtttca aaatggggaa cggcgaacca
     1321 gggcgcgtgt gcaatgcttg tcgtgtccac cagtcccatc agcagtgcca aaacataacc
     1381 cgaagcgaca ccgatcaaga tgggaatcag cttcatcatc ctgctgccga aaaccgatac
     1441 gatggcggta acggcaaagg taaagccgga aagaatcagc gaatcggtat agtcgatgac
     1501 ctgtttgccg tcagcctgac ccattgccat gctgcttgcc gccacggcaa cagacagacc
     1561 gatgaccatg atgacagggc cggtgacgac cggcggcagc agtttgtgta ccgccgccag
     1621 tccgcgccaa cggatcagcg cggcaaacac aaaatacata aagccggcgg caaacagccc
     1681 gaacatggtg gaaggcagcc cccattcgcc gacggagtag ataatcggcg cgataaaggc
     1741 aaacgaagaa ccgagaaaaa tcggtacttt gcgtttggtt gtgatttgga acagcagcgt
     1801 tcccaagcct gcgcccaaaa gcgcaagagc cggattcaga ccggtcagca ggggaaccag
     1861 caccattgcg ccgaatgcca caaataaaat ctgcgcgccg gaaacggcaa gtttcagttg
     1921 gttcatacca tcttctttca atcggttcaa acggcacgga ttatactggt ttgtcgaggg
     1981 gctgcgtaaa aatacatacg tcgggcaaag atatgccgtc tgaaaggcaa cgtctgtctg
     2041 ttgcaggatg aaagcaggag aaatggagta taatccgaca ctttccatat tcaatccgca
     2101 agccgtcccg aacccttatt tcagacggca ttttccggta atacggcatg acagaaaaat
     2161 cccataaaaa aacagcaaaa ggcagggcgg gaagcccgtc cccaacgtcc gcacgcaaca
     2221 agaaagccga caacggcgcg cggggcaata aagtttccga gcggctcaag gcggtcaaag
     2281 agctgcagaa aaccgaaacc aaaaaggcgc gtcccgaaca tgtcgtcaac ctgattgccg
     2341 acgcgctgtg gctgatgggt ttggcggcaa ccctgtattt ggcgatttcc ctgatcagtt
     2401 tcgatatggg cgatccgtct tggtcgcaca gttcaccggt tgtggaagat gccgccaatt
     2461 ggggcggact gttcggcgcg tatgttgccg atgtcggcta ttatcttttc ggctggtcgt
     2521 tctggtggtg gatagcggct gcctgcgtcg tgctgtataa aaatttccgt ctgcacgcaa
     2581 aacagacgga aaacgaggca tacaaccaca aaatcgctgc cgccgcgctg tttgtcctga
     2641 cggtcttcag ccccgtcttg gagtattttg tgctgggcgg aaaatatgcc gactccctgc
     2701 ctgtcggagc aggcggtatg gtcggcatac gtgtcggcgc agtgtttgcg tggctgttgg
     2761 ggaaatcggg cagcctgctg attatcctgg ttgttctgct gttgtcgttg tccctgctgg
     2821 tgcagatttc atggctggaa tttttgaacg gtgcgggcag ggcggttcaa aaccgcctga
     2881 gtgccttatc cggcaaggtc atggctttga gaaaacgccg gccgaatacc aaaacagacg
     2941 gtgtcgatac ccaaaataca cggcgcatgg tgaaagaagc caaaaacatc actgccaaac
     3001 ccgttgcctt gcccgaaggc agcagcagca accgcaaatc cgtcgcggtt tccgttgcgc
     3061 cgccgcctaa aatccaacct tcgctgtttg aagacaatga agtacagcag aacggcgaat
     3121 accacaagcc tacattgaac ctattgcgga ttcccgacag cgagccggtc agcatcaatc
     3181 ccgccgaatt ggagcgcact gccgaactaa tcgaatccaa actggcagaa ttcggcatcg
     3241 gcgtacaagt cgtatccgcg acatccggcc ccgtcatcac gcgctacgaa atcgaacccg
     3301 cgcaaggtgt caaaggcagc caaattgttg ccctgtcgaa agatttggca cgctctatgt
     3361 cgctgcaatc cgtgcgtatc gtcgaaacca ttgcgggcaa aaacacgatg ggcatcgagt
     3421 tgcccaacga caaacgccaa gacgtgatgt tgagcgaaat cttgtcctcg cccgtgtttg
     3481 ccgaagccaa atccaagctg accgtcgcgc tgggcaaaga catcgccggt acgcccgtcg
     3541 tcggcgactt ggcgaaaatg ccgcaccttt tggtcgccgg catgaccggt tcgggcaaat
     3601 ccgtcggcgt gaacggcatg attatgtcta tgcttttcaa agctacgccc gaagaagtcc
     3661 gcttcattat gatagacccg aaaatgctcg agttgagcat ttacgacggc attccgcacc
     3721 tactctgtcc cgtcgttacc gatatgcgcg aagcagggca ggcgttgaac tggtgcgtcg
     3781 ccgaaatgga aaaacgctac cgcctgctct cccacgccgg tgtacgcaat ctggaaggct
     3841 tcaaccaaaa agtcgaacaa gccaaagcgg caggcaagcc gctgctcaat ccgttcagcc
     3901 tgaacctcga cgaacctgag ccactggaaa aactgccgat gatcgtggtc gttatcgacg
     3961 aacttgccga cctgatgatg accgaacgca aagctgtcga gcagcaaatc gcccgcctcg
     4021 cccaaaaagc gcgcgccgcc ggcatacaca tgattgtcgc cacccaacgc ccaagcgtcg
     4081 atgtcgtaac cggtctgatt aaagccaaca tcccgacgcg tatggcgttt accgtgcaaa
     4141 gcaaaatcga cagccgtacc atcctcgacc aaatgggcgc ggacgaactg ctcaaatatg
     4201 gcgattcgct gttcctgcaa ccgggcagtg ccgaaccgac ccgcctgcaa ggcacgtttg
     4261 tttcagacgg cgaagtgcat caagtcgtca actacgtcaa atcgcaagcc ccagccgact
     4321 atattgaagg tctgctcagc ggcgaagccg cgctggaaac tgccaatatc gttaatccga
     4381 atgcagacag cgacgaatta ttcgatcagg cagtcgccta tgttttggaa agcaaaaaaa
     4441 cctccatttc gtctttgcag cggcagttgc gtatcggcta taaccgcgcg gcaaacctga
     4501 tggaggcact ggaaaatgcg ggtatcgttt ctccctccga cctcaacggc agccgtaaaa
     4561 ttttggcgca caaggaccat ttatagcccg tatcgc
//
LOCUS       DAXDSD010000121         4370 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXDSD010000121 DAXDSD010000000
VERSION     DAXDSD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.121"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..1967)
                     /locus_tag="ACFB2Q_001917"
     CDS             complement(<1..1967)
                     /locus_tag="ACFB2Q_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFC0918883.1"
                     /translation="MSKKTPLSQGFIARVAAGVRYAFTGNADGWFDAGEPPAPAAQQA
                     EGRRFDYEPFYNVGHSKPREREAVGFAQLRALADNYDVLRLVIEARKDQMECLKWTIQ
                     KRDVESTEDDESQRKDRKVDEAVAFFRSPDKEHTWADWLRILLEDLFVIDAPCIYPRK
                     TLGGGLYALEVMDGATIKRVLDNTGRMPLPPDTAYQQILHGMAAVDYTADELIYRSRN
                     NRSYKVYGYSPVEQIIMTVNIALKRQVHALEYYTAGSVPDALVGVPETWSADDIRRFQ
                     EYWDLLLSGETAQRRKMRFVPGELSRNFRETKQPPLKDVYDEWLARVVCFAFSVEPTP
                     FVAQVNRSVAETSREQSLSDGMGSLKNWVKALIDDVLARYMDMAAYEFVWKGEESLNP
                     KEQAEIYAIYKNAGILTADEIRAELGKEPLPGQGQPEPDKQDGRKPEEPPNQGAEKLG
                     KSESPMSEDESAALIEAYLLTRIDGLAEQIAALIEGAAVDWQAGDLAAELSRAAGVVA
                     NGLDFGDWSGLSDVVEPIIRRVAEDGAVAALLRVMPEPAAGMVTNIRSRAVKWAHERA
                     AEMVGMKRAGGGLVRNPAAEWQITEGTREMIRAQVAEAMRNGDSVQELAGRLKESHAF
                     GNARARTIARTETAMADGMGNLIGWEGTGLVA"
     gene            complement(1964..3385)
                     /gene="terL"
                     /locus_tag="ACFB2Q_001918"
     CDS             complement(1964..3385)
                     /gene="terL"
                     /locus_tag="ACFB2Q_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC0918884.1"
                     /translation="MALGQFDDVETSVIRSLSSASLYMFTRRMFYQRRGYVWQRANHH
                     APICNALERVFNGETKRLIINIPPRYSKTEIAVVNFIAWAMGRVPDCEFIHASYSAAL
                     AVNNSVQIRNLVQHEEYRAIFPDLALAGESGHHWKTTAGGVMYATGAGGTITGFGAGR
                     HREGFGGCIIIDDPHKADEARSEVRRQNVIDWFQNTVESRKNSPDTPIILIMQRLHEK
                     DLAGWLLDGGNGEEWEHLCLPAIQEDGTALWPEKHDIETLRRMEQAAPYVFAGQYLQK
                     PAPPDGGTFKPDNLQFVKALPAGNIRWVRAWDLASTANGGDYTAGGRLGVTEDGRYII
                     ANVVRGRYGADERDRILRNTAQKDGVKTKISIPQDPGQAGKSQTLYLTRQLAGFSVSA
                     GPESGDKVTRAGPFAAQVNIGNVMVLDDGTWDTDALIAEMRMFPNGRHDDQIDCLGRA
                     FGELLDTRTGMIDFLRSQVEAVK"
     gene            complement(3447..3896)
                     /locus_tag="ACFB2Q_001919"
     CDS             complement(3447..3896)
                     /locus_tag="ACFB2Q_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918885.1"
                     /translation="MSGTKRKLGRPTDYTKDMADKICEKIANGRSLRSICAEDGVPPM
                     KTIYRWLEANEEFRHQYARAREKQADYFAEEIIEIADSAQAESAAVSKAKLQIDARKW
                     AASKIAPKKYGDKTELDVKSGDGSMRAAVRLDAEEYRKIAEDVLRRV"
     gene            complement(4189..>4370)
                     /locus_tag="ACFB2Q_001920"
     CDS             complement(4189..>4370)
                     /locus_tag="ACFB2Q_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFC0918886.1"
                     /translation="PLKQLGCRQAVTMWTVWAETRSRLKAAANALEALSAHADAEHAE
                     KIRPILPEIRNLSAV"
BASE COUNT          854 a         1381 c         1100 g         1035 t
ORIGIN      
        1 gcaaccagcc ccgttccttc ccagcctatc aggttgccca taccgtccgc catcgccgtt
       61 tccgttcggg caatggttcg ggcgcgtgcg ttgccgaaag catgggattc tttcaggcgg
      121 cctgccaatt cctgcacact gtcgccgttt cgcatggctt cggctacttg ggcgcgtatc
      181 atttcgcgcg tcccttcggt gatttgccac tcggcggcag gatttcggac aagcccgccg
      241 cccgcccgct tcatgccgac catttcggcg gcgcgttcat gcgcccactt gacggcgcgg
      301 ctgcgaatgt tcgtaaccat accggcggca ggttcaggca ttacgcgcaa caaggcggca
      361 accgccccgt cttccgcaac acgcctgatt atcggctcga ccacatcgga caatcccgac
      421 caatcgccaa aatccaagcc gtttgcaaca acccctgccg cgcggctcaa ttccgccgcc
      481 aaatccccgg cctgccaatc gacggccgca ccctcaatca gcgccgcgat ttgttcggcc
      541 aagccgtcaa tgcgtgtcag caaataagcc tcaataagcg cggcagattc gtcttcgctc
      601 atcgggcttt ccgactttcc caacttttca gccccttggt tcggcggctc ttcgggcttt
      661 cggccgtctt gcttatccgg ttcaggctgc ccctgccccg gtaacggctc cttgcccagt
      721 tcggcgcgga tttcgtcggc ggtcaagatg cctgcgtttt tgtagatggc gtagatttca
      781 gcctgttctt tcgggttgag cgattcctcc cccttccaga caaactcata cgccgccata
      841 tccatgtaac gggcaagcac gtcgtcaatc agggctttta cccagttttt caggctgccc
      901 atgccgtctg aaagcgactg ctcgcggctc gtctctgcca cgctgcggtt tacctgcgcc
      961 acgaacggcg taggctcgac actaaacgca aagcagacga cacgcgccag ccattcgtcg
     1021 taaacgtcct tcaacggcgg ctgcttcgtc tcgcggaagt ttcgggacaa ctcgcccggc
     1081 acgaaacgca ttttgcgccg ctgcgccgtt tcgcccgaca gcagcaaatc ccagtattct
     1141 tgaaaccgcc tgatgtcgtc cgccgaccac gtttcaggca cgccgactaa agcatcgggc
     1201 acgctgcccg ccgtgtagta ttccagcgcg tgaacctgcc gttttaaggc gatattcacg
     1261 gtcatgatga tttgctcgac gggcgaatag ccgtaaacct tgtaacttcg gttattccgc
     1321 gaacggtaaa tcaactcgtc agccgtgtaa tcgaccgccg ccatgccgtg caggatttgc
     1381 tgatacgccg tatcgggcgg taacggcata cgccccgtat tgtccaaaac gcgcttaatc
     1441 gtcgccccgt ccatcacttc gagggcgtac aagccgccgc ccagtgtttt gcgcgggtag
     1501 atgcacggcg cgtcaataac aaacaggtct tccagcaaga tgcgcagcca gtccgcccac
     1561 gtatgttctt tatcgggcga ccggaagaac gcaacggctt catcgacctt tcggtctttc
     1621 cgttgcgatt cgtcgtcttc ggttgattcg acgtcgcgct tttggattgt ccacttaagg
     1681 cactccattt ggtctttacg cgcctcgatc accaaacgca atacatcgta gttgtcggca
     1741 agggcgcgta attgcgcaaa gcctaccgct tcacgttcgc gcggcttgga atgcccgacg
     1801 ttgtagaacg gctcgtaatc aaaccgccgc ccctctgcct gctgcgcggc aggggccgga
     1861 ggctcgcccg cgtcgaacca cccgtccgcg ttgccggtaa aggcgtaacg gacgccggcg
     1921 gcaacgcggg caataaagcc ttgcgataaa ggtgtctttt tactcatttc acagcctcga
     1981 cctgcgatcg caggaaatca atcatgcccg tccgggtatc cagcagctcg ccaaacgcac
     2041 ggcccaaaca gtctatttgg tcgtcatgcc ggccgttcgg gaacatgcgc atttccgcaa
     2101 tcagcgcgtc cgtgtcccat gtgccgtcgt ccagcaccat cacattgccg atgttgacct
     2161 gtgccgcgaa cggtccggcg cgtgtaacct tgtcgcccga ttcggggccg gcggatacgg
     2221 aaaaacccgc caactggcgg gttaggtata gtgtttggga tttgcctgcc tgaccggggt
     2281 cttgcgggat ggatattttc gttttcacgc cgtctttttg cgccgtgttg cgtaatatcc
     2341 tgtcccgctc gtccgcgccg taccggccgc gcacgacgtt ggcgatgata taccgcccgt
     2401 cttccgtaac gccaagcctg ccgcctgccg tgtagtcgcc gccgtttgcg gttgaagcca
     2461 agtcccacgc gcgtacccat ctgatattcc cggcaggcaa cgccttgaca aattgcaggt
     2521 tgtcaggctt gaacgtaccg ccgtcaggcg gcgcaggttt ttgcaaatac tgcccggcaa
     2581 acacatacgg cgcggcctgc tccattcggc gcaatgtttc aatatcatgc ttttcaggcc
     2641 acaacgccgt gccgtcttcc tgaatggcag gcaggcacaa atgctcccac tcttcgccgt
     2701 tgccgccgtc aagcagccag cccgccaagt ctttctcgtg caggcgttgc ataatcagga
     2761 taatcggcgt gtcagggctg ttcttccggg attcgaccgt gttttgaaac cagtcgatga
     2821 cgttctgccg cctgacctcg cttcgcgctt catctgcttt gtgcgggtcg tcaatgatga
     2881 tgcagccgcc gaatccctcc cgatgcctgc ccgcaccgaa acctgtaatc gtaccgcccg
     2941 cacctgttgc gtacatcacg ccgcctgcgg ttgttttcca gtgatggccg ctttcgcctg
     3001 ccagtgccaa atcaggaaaa atcgcccgat actcttcgtg ttgcaccaag ttccgaatct
     3061 gtacggagtt attgaccgcc agcgccgccg aatagctcgc gtggataaac tcgcaatcag
     3121 gcacgcgccc catcgcccac gcgataaagt tcacgaccgc gatttccgtt ttcgagtatc
     3181 gcggcggaat attgataatc aggcgtttcg tttcgccgtt gaaaacacgc tcgagcgcgt
     3241 tgcagattgg cgcatggtga ttcgcccgct gccaaacata gccgcgcctt tgataaaaca
     3301 tccggcgcgt gaacatatac aggcttgcag aacttaaact gcgaattact gatgtttcaa
     3361 catcgtcgaa ttgccctagt gccatttcat tctaacttcc tttggagaaa cggccataca
     3421 gcccctctta tgattagcgt tttatgctaa accctacgca aaacatcttc cgctatcttg
     3481 cgatattcct cagcatcaag ccgtacagcc gccctcatgc tcccatcgcc cgatttaacg
     3541 tcaagttccg tcttatcgcc gtatttcttc ggcgcaatct tggaagccgc ccactttcgg
     3601 gcatctatct gcaatttcgc ctttgaaacc gccgcgctct ctgcttgtgc actatcggca
     3661 atctcgatga tttcttcagc gaaatagtcc gcctgctttt ctctcgcgcg cgcgtattgg
     3721 tggcgaaatt cttcattagc ttccaaccaa cggtaaatag ttttcattgg cggcacacca
     3781 tcttcggcgc atattgaacg taggcttctg ccatttgcga ttttttcgca tatcttatcg
     3841 gccatgtctt tcgtgtaatc tgtcggacgg cctaatttgc gtttcgtgcc gctcataaac
     3901 cctcctcaaa aaagaaaccg tctaactctg acccctctca gaattagacg gcaaacacac
     3961 actcgccaca taggaaaaac ggaacgccgc tacctgtaca ggcagaagct caaattcgga
     4021 cagcctaaaa cgcaaaaacc cgcacattgt tatgtacggg cttaaaaatt catatccttc
     4081 gggcgtgcga aaatccccgc aagggtaacg atttgaatta tacacctatt gccggaaaaa
     4141 acaacaggcc gtctgaagat tcagacggca tttgtattcc ctactgcatc aaaccgccga
     4201 caggttgcgg atttcgggca gtatcgggcg gattttttct gcgtgttccg cgtcggcgtg
     4261 tgcgcttaag gcttcgaggg cgtttgcggc ggctttgagg cggctgcgcg tttccgccca
     4321 gaccgtccac atcgtaaccg cctgcctgca gccgagctgc ttcagcggtg
//
LOCUS       DAXDSD010000122         4365 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXDSD010000122 DAXDSD010000000
VERSION     DAXDSD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.122"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(106..1137)
                     /gene="gap"
                     /locus_tag="ACFB2Q_001921"
     CDS             complement(106..1137)
                     /gene="gap"
                     /locus_tag="ACFB2Q_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC0918887.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(1336..2175)
                     /locus_tag="ACFB2Q_001922"
     CDS             complement(1336..2175)
                     /locus_tag="ACFB2Q_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC0918888.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPEKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            2321..4297
                     /locus_tag="ACFB2Q_001923"
     CDS             2321..4297
                     /locus_tag="ACFB2Q_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC0918889.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
BASE COUNT          773 a         1022 c         1473 g         1097 t
ORIGIN      
        1 tggaaatgac gacagccggg taaaatcgcg gtcaaaaatg aaaagtgccg tctgaaactt
       61 gaaaacatcg ggtttcagac ggcattttgt ttgacggttt gttgcttatt tgagcgggcg
      121 cacttcaagt ccgaacatac ggcgtgcggt gttcagcatt tggcagctga agccccattc
      181 gttgtcatac cacgcgaaca ctttgaccat gttgccgtcg acgactttgg tcagcgttgc
      241 gtcgaagtgg ctggcttcgg tggtgtggtt gaagtccatg gaaaccaatg gcagggtgtt
      301 gtagcccaaa acgcctttga gcgcgcctgc ttccgaggcg gctttcatca gtgcgttgat
      361 ttcttcgact gtggtgtcgc gcgcggcttg gaagctcaaa tctaccaagg atacgttgac
      421 ggtcggcacg cggatggcaa gcccgtcgag tctgcctttc aattcgggca agaccaaacc
      481 gacggctttt gccgcgccgg ttttggtcgg aatcatgttt tccacgccgc tgcgggcgcg
      541 gcgcaggtct ttgtggcgca cgtcggtaac ggtttggtcg ttggtcagtg cgtggatggt
      601 ggtcattgcg cctttgacga tgccgacgct ttcgctcaac actttggcaa ccggcgagag
      661 gcagttggtg gtgcaggaag cgttggaaac gacggtcatg tcggcggtca ggacgctgtc
      721 gttcacgccg tacacgacgg ttgcatcgac atcgtcgccg cccggtgcgg aaatgaggac
      781 ttttttcgcg ccgctttcga ggtggatttt ggctttttct ttgctggtga acgcgccggt
      841 gcattccatg accaaatcga caccgagttc tttccacggc agttcggcag ggttgcgggt
      901 cgagaagaag gggattttgt cgccgttgac gatgaggttg ccgccgtcgt gggatacgtc
      961 ggcttcaaag cgtccgtgta cggtgtcgaa tttggtcaga tgggcgttgg tttcaaggct
     1021 gccgctggcg ttgacggcga cgatttggag ttggtcttga atctgatagt cgtagatggc
     1081 gcgcaaaacc tggcggccga tgcgtccgta gccgttgatg gcgactttga tgcccatggt
     1141 ttgttccttt gttgagggtt gggtagattt tcggggcgga ttatagcaaa tttgtagtgg
     1201 cgtgtaatta atattttatt gaaaacggca cggccggaag ggtgggcggt aagatacgga
     1261 cggcacgggt acggcggacg gagagcttga taaaatgccg tctgaagcgg cttcagacgg
     1321 catgtcgggt aagggtcagg aggcggtatt ctgtgcggct tcctgtttgg ctttgtattg
     1381 tttgaggtat tcgagggcgg cggctttttc gctgtcgctg ccgtatttca tatcgcgttg
     1441 ggcgcggcgc aattcggcgc gttcgcgggc ttcggctatc tgttttgcct gatagccttg
     1501 gcggttgtcg gcggcggcga ggcgatcctg ttgggtttgc gcttttgcca tggctttggc
     1561 gatgaggtcg gcggggttga acgccggttt ttccggtgtg tcgggcgttt gcggacgcgc
     1621 gttgcggacg gcggcttcgc gttcggcaag catagccttg cgttcgtcgg cttcgcgctg
     1681 tttgcgttcg ttgcgtttga ggtagcgcgc acgcgcgtgt tcggcggcgg caaaacggct
     1741 gtcgtcggac aggctgaaac ggcgcgcgcg cggcaggacg gtgtcggcaa cgggctgcat
     1801 atggatgcag tccacggggc agggggcgac gcagagtccg cagccggtgc attcgtcggt
     1861 gatgacggtg tgcataagtt tgcccgcgcc cataatggca tcggcagggc aggcgcggat
     1921 gcaggcggtg cagccgatac aggcggtttc gtctatccgg gcgagtgctt tggcttgggt
     1981 tttggcaggt gcgacaaggg gtttgccgag cagggcggca aggtcgcgga cgacggttgc
     2041 gccgcccggg gcgcagaggt tgtgcgcctc gccccgcagc atcgcgcggg cgtagggcag
     2101 gcagccttca tagccgcatt cgcggcattg ggtttggggg aggagtcggt ctaaatcggc
     2161 ggcggtggcg gtcattgtgc ggtggtgcgg gtcaaaaggg cgtattttat cagaattgta
     2221 tgccgcgccc gtttcggatg gtgcgcggtg ttttgttata atacggcggc gtatgccgtt
     2281 tcagacggca tttttctgta ttttcctgtt cggacggtcc atgaacgaat tttcgcttgc
     2341 ccctattgtg gttgttctgc tggtgtcggt cattacggtg atcctgtgcc gcaagttcaa
     2401 cattccctcc atgctgggct acctgctggt gggctttttg gcggggcccg gtatgctcag
     2461 cctgattccg aaaagccatg cgacggatta tttgggcgaa atcgggattg tgttcctgat
     2521 gttcagcatc ggtttggagt tttcattgcc caagctgagg gcgatgaggc ggctggtgtt
     2581 cggtctgggc ggtttgcagg tcggcgttac gatgctgtcg gtaatgggca tactgatgct
     2641 gacgggcgtg ccgttcaatt gggcgtttgc cgtatcgggc gcgttggcga tgtcgtccac
     2701 ggcgattgtg agccggattt tgtcggaaaa gacggagttg gggcagccgc acggtcagat
     2761 ggcgatgggc gtgctgctga tgcaggacat cgccgtcgtg ccgctgatga ttctgattcc
     2821 cgcgctggct ggcggagggg acggaaatat ttgggcggcc ttgggtttgg cgtttgcaaa
     2881 aatgctgctg acgctggggc tgctgttttt cgtcggcagc aaaattatgt cgcgatggtt
     2941 caggatggtg gcaaaacgca aatcgtccga actctttatg atcaatgtgc tgctggtaac
     3001 cttgggtgtg gcttatctga ctgagctgga aggtttgtct atggcgttgg gcgcattcgt
     3061 tgccggcatg ttgctttcgg aaacggaata ccgtttccaa gtcgaagacg acattcgccc
     3121 gttccgcgat attttgctcg gcttcttctt tatcacggtc ggcatgaagc tggacattca
     3181 ggcattgatc ggcggctggc ggcaggtatt gatgctgttg gcaatgctgc tggtgttgaa
     3241 ggcattggtt gtgtttgcca ttgcattcaa aatgaagcat tcggtcggcg acagcctcaa
     3301 aacggctttg tatctcgcgc aaggcggcga gttcggcttc gtgatgctgg ccattgccgg
     3361 gcagcttgat atggtttcgc cagaattgga acaggcggcg acggcggcgg ttctgctgtc
     3421 gatgattatc gcgcccttcc tcttgggcgg cagcgatgcg ctggtcgggc gcttggtcaa
     3481 gtcaagctgg gacatgaagt cgctcgatct gcacagcatg ttggtggaag cgatgagcaa
     3541 gtctgatcat gtgctgattg tcggcttcgg caggggcggg cagacggtcg gacgcgtcct
     3601 cgcccaagag gatattccgt atttcgcgct cgacttggac atcgcacgcg tgcaggtagc
     3661 cagaagtgca ggcgagccgg tgtcgttcgg cgatgcgaaa cgcagggaag tattggaagc
     3721 cgccggtctg ggacgggcga aaatggtggt ggttacgctc aacaatatgc acgaaacgca
     3781 acacgtttta gacaatgtgc tgtccatgca tcccaatatg cccgtatatg cgcgcgccac
     3841 caatgacgat tatgtgaaaa cgtttaccga tatgggtgcg gaagaagccg tgtcggacac
     3901 caaagaaacc ggactcgtgc tggcaggcta tgcgatgttg ggtaacggcg cgtcgtaccg
     3961 gcacgtctat cagacgatgg caaatatccg ccacagccgt tatgccgcgt tggagggact
     4021 gtttgtcggt agtgatgatg aggcaggatt cggcgaaaat ggcgaaaccg tccgccacgc
     4081 ctttcctttg gctgcagaag catacgccgt cggcaaaaca gtcggcacgc ttccgatggc
     4141 ggcttacggc atcaaactct tgttcgtccg ccgccgcacc ggccggattg aaaacccgga
     4201 tgcctcgttt acattggaag gcggtgacgt gttggtggtc gcaggcaaaa aagaagaaat
     4261 tatctctttt gaaaactgga gcttgcaggg aatataaatg aaatgccgaa ataaggcttg
     4321 cgccattccc ggttatttgg tctaatagcg ttttcgcaaa tcgca
//
LOCUS       DAXDSD010000123         4349 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXDSD010000123 DAXDSD010000000
VERSION     DAXDSD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.123"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..1179)
                     /locus_tag="ACFB2Q_001924"
     CDS             complement(<1..1179)
                     /locus_tag="ACFB2Q_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918890.1"
                     /translation="MKQKKTVQCILLGFAAASMHAQGAAAANSGTIEKTDKYTLVLAK
                     QGQENNYTLNGGTEVKPLNSLIIAANGGTNNITIKGKLADGPADAPPTIDNNSIERNI
                     NKNGYTYAWQNWSGAVMLVDQSYEGENKVTFENVTIAAHNAPAGILSDDRHKSSSLAP
                     AMLAFKGRNTINMDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKSGKDK
                     GQGITANHYGNSDINFNKASPNITTMEFKGGVNIKIDRNGQEEAESNGFGFYSSRKLG
                     NKKQIPEGSKMEAIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGGEGKV
                     VKIKGDIFAYNGGSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYELTPD
                     T"
     gene            1376..1451
                     /locus_tag="ACFB2Q_001925"
     tRNA            1376..1451
                     /locus_tag="ACFB2Q_001925"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1410..1412,aa:Glu,seq:ttc)
     gene            complement(1667..1819)
                     /locus_tag="ACFB2Q_001926"
     CDS             complement(1667..1819)
                     /locus_tag="ACFB2Q_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918891.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            1879..2685
                     /gene="thiD"
                     /locus_tag="ACFB2Q_001927"
     CDS             1879..2685
                     /gene="thiD"
                     /locus_tag="ACFB2Q_001927"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC0918892.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2743..3612)
                     /gene="tehB"
                     /locus_tag="ACFB2Q_001928"
     CDS             complement(2743..3612)
                     /gene="tehB"
                     /locus_tag="ACFB2Q_001928"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC0918893.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            3725..4162
                     /gene="rnhA"
                     /locus_tag="ACFB2Q_001929"
     CDS             3725..4162
                     /gene="rnhA"
                     /locus_tag="ACFB2Q_001929"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC0918894.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1002 a         1227 c         1035 g         1085 t
ORIGIN      
        1 tgtatcgggg gttaattcat atccgtccgc atccacggtt aaggcaaaca tatctttccc
       61 tttggcaaag ctccttttcc cgatatgggc ttccccttca aaataagagt ctttgttggc
      121 aaggttcacg ctcacgctgc cgccgttgta ggcgaaaata tcgcctttga ttttgacaac
      181 cttgccttcc ccgccgacaa cctcgacttt gctgtatttc ccgtcaatcg caaacgcgct
      241 gcctatgctt ttgggcctgc cttgctcgtc ataaaccggc gttgcaacga tatcgacgtt
      301 cccacggaaa atcgcttcca tcttagaacc ttccggaatc tgcttcttat tgcctaattt
      361 gcggcttgaa tagaagccaa aaccgttgct ttccgcctct tcctgtccgt tcctatcgat
      421 tttaatgttt acaccgccct tgaactccat tgtggtgatg tttggagaag ccttgttgaa
      481 gttgatgtct gagttaccat agtggttggc ggtaatgccc tgtcctttat cttttcctga
      541 tttgatgttg atattcagcg tcgagccttc ttcggaaaca agacgatatt ctcccatttt
      601 ttcaccatta ttaagcaata ggataccttc gttactagag ttggcatttg agtccgcgtc
      661 catattgatg gtattgcgcc ctttaaaggc aagcatcgcc ggcgccaagc tactggattt
      721 atgcctatca tcagacaaaa tgccggcagg cgcgttatgg gcggctatgg ttacgttttc
      781 aaaagtaacc ttattttctc cctcatagct ttgatccacc aacatcaccg cgccgctcca
      841 attctgccat gcgtacgtgt aaccgttttt atttatattt ctttcaatcg agttattgtc
      901 aatcgtcggc ggggcatcgg caggaccgtc tgccaatttg ccttttatcg taatgttgtt
      961 cgtaccgccg ttggcggcaa tgatgaggga atttaagggt ttgacttcgg ttccgccatt
     1021 aagggtatag ttgttctctt gcccttgttt tgcgaggacg agggtgtatt tgtctgtctt
     1081 ctctatcgtt ccactgtttg cagcggccgc cccttgcgcg tgcatagaag cggccgcgaa
     1141 gccaagtaag atgcactgaa ctgtcttctt ttgtttcatc ggtttttcca ttttgaaggt
     1201 atgtgatttt tatcattata tgcttaaatc attgacacgc ccccaaaaaa aatgaattgc
     1261 ttgatattcc aatgtttttt atcttgtgat acggtcagac aaacgccccc tgacattcgt
     1321 ttagacggca tcgtattgct aaatttctat aagtatgtat aatgtccgtt tccacgcgcc
     1381 catcgtctag aggcctagga cactgccctt tcacggcggc aaccggggtt cgaatccccg
     1441 tgggcgtgcc aattcaaaaa cctgcttgtt tcaagcaggt tttttattat gaatcgttat
     1501 tcccgcaatt tttcgtcatt gccgtgaaaa cgggaatcta gaacctcaaa ctttcagata
     1561 atctttgaat attgctgttg ttctaaggtc cggattcccg cctgcgcggg aatgacggaa
     1621 ttttaggttt ctgtttttgc gggaatgacg aatgaagcgt gccggtttat actcgccgca
     1681 acacgcggtt cagacggcat cgccctcttt tttcattatc agttgctacg atgcaccccg
     1741 ctgccttgcc ctgtgccttg tcctgcaata cgacataata atgcaccaca aaccccgcgc
     1801 tgcggttttc agacggcatt gcctgctttt ttacaggcag tagccctttt tatcggacgc
     1861 aatattaagg aggaacaaat gaaaggctct tttgtgcaga cgcttgccat cgccggttcg
     1921 gattcgggcg gcggtgcggg cattcaggcg gatttgaaaa cgtttcagat gcgcggcgtg
     1981 ttcggaacgt gtgtcatcac cgccgttacc gcgcaaaata ccttgggcgt gtcggcggtt
     2041 catctcgtcc cgaccgaaac catcaccgca caaatccaag caatcaggga agactttgac
     2101 atccgcgcct acaaaatcgg tatgctcggc acggcggaaa tcatcgaatg cgttgccgac
     2161 aagctgaaac actgcagctt tggcaggcgc gtactcgacc ctgtgatgat tgccaaaggc
     2221 ggtgcgccgc tgttgcagga ttccgccgtt gcggcactga cgcgcctgct gctgccggac
     2281 acggacatcc tgacccccaa cctgcccgag gcggaagcct tgacgggcgt gcatatcgaa
     2341 aaccgtaaag atgcggaacg tgcggcaaaa atcctgcttg attacggtgt caaaaatgtc
     2401 attatcaaag gcggacattt gaacggcagc acaagcggac gctgcacgga ttggctgttt
     2461 acgcaaaacg aaacgctgga attggacagc ccgcgctttc cgaccgccca cacgcacggc
     2521 acgggctgca cgttttccgc ctgcattacc gccgagttgg caaaaggctt agacgtttgc
     2581 aaagccgtac agactgccaa ggcctacatc acggcggcaa tctcaaaccc tttggaaatc
     2641 ggcgcgggac acgggccggt caatcattgg gcgtatcggg actagacgta aaaatgccgt
     2701 ctgaacgcct tttcgggttc agacggcatc gtccggcgcg gcttatcctg gttttttcgc
     2761 cagcatagtt acaaatttaa accgtatcgg gttgccgttt tcgtctttgg cgtgcatcgc
     2821 gcccaattct tctttatatt cgaccagctc ccaatcccga taataatcct tcagctcgcc
     2881 ctctttaaat ttgaagggga acggcatcgg gcaggggaaa tccgccgtat ccattgccga
     2941 tacgattaag ttgtacccgc ccgccgccgt atgcgcctgc atatcggcaa tcacgtcggg
     3001 cacgcgctgc ggcatcagga acatcagcac caccgttgcc acaatataat caaactcgcc
     3061 ctgaagggcg gcggcgttca aatcatattc cagcgtgcgg acgttcaaac cctccgcctc
     3121 tgccagttcc gccacgtttg ccaacgcggc gggattgcaa tcggcagcgg ttacatcaaa
     3181 ccccttcaaa cccaggaaca gcgcgttgcg cccctgtccg cagcccatat ccaacgccct
     3241 gcccgccggt acggtatcct gtgccgccgc gaccgcagaa tgcgtggcac tcatcccgta
     3301 ttttttgtga aaatagtctg ccgccgcgca atacagcgac aaacggattt cggcatcgtc
     3361 cgttttcggt ttgaccgaaa acacctgctg cggcgcaaac acgcaatcgc cgctttccgc
     3421 cgaccaaacc tctgccgccc cgtccggtgc acggacttcg acatcgccct gcaacacatt
     3481 caggcagacc cattcccctt cctcggacga ataacccgac aacaaaactt ccggcaggtt
     3541 ttccactttc catacaggca tttgtccgaa acaaaacaac tcgccacttt gacccactat
     3601 ccgctccttc atattcaaaa ataaagttgc acattatatg cctatttcaa tccgccgcaa
     3661 tctttcagac ggcacgacgc acaaaccgct tataatcacg ccggacacca cacaaaggca
     3721 cgatatggac acacccgttt acctctacac ggacggcgcg tgcaaaggca atcccggcgc
     3781 gggcggctgg ggcgtattaa tgcgctacgg cagccgcgaa aaagaacttt tcggcggcga
     3841 agcgcaaacc accaacaacc gcatggagct gaccgccgtt atcgaagggc tgaaatcgct
     3901 caaacgccgc tgcaccgtca tcatctgcac cgactcgcaa tacgtcaaaa acggcatgga
     3961 aaactggata cacggttgga agcgcaacgg ctggaaaacc gccgccaaac agcccgtcaa
     4021 aaacgacgac ttgtggcaag aactcgacgc tctggtcggg cagcatcaag tcagctggac
     4081 ttgggttaaa ggacacgcgg gacacgcgga aaacgaacgc gccgacgatt tggcaaaccg
     4141 tggcgcggcg cagttttctt gattgccgca ccgacaaaaa tgccgtctga aaccgctaat
     4201 gggcttcaga cggcatcgtc ctccaccgtc attcccgcgc aggcgggaat ccagaacgtc
     4261 gggcaacggc aatattcaaa agccgtctga aaatttaaaa gttctagatt cccgttttca
     4321 cgggaatgac gaaaagttgc ggacagcag
//
LOCUS       DAXDSD010000124         4334 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXDSD010000124 DAXDSD010000000
VERSION     DAXDSD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.124"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            372..1292
                     /gene="ribF"
                     /locus_tag="ACFB2Q_001930"
     CDS             372..1292
                     /gene="ribF"
                     /locus_tag="ACFB2Q_001930"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC0918895.1"
                     /translation="MKIRPGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAARTGKTPPCRISPLRTKLGLLEKTGCVDAAWVLRFDRNF
                     SEISAQAFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDGGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            1434..4223
                     /gene="ileS"
                     /locus_tag="ACFB2Q_001931"
     CDS             1434..4223
                     /gene="ileS"
                     /locus_tag="ACFB2Q_001931"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC0918896.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGGYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1043 a         1227 c         1170 g          894 t
ORIGIN      
        1 agattttcac tgatgttgaa gggcggatta tatcgggttc cgggcggtgt ttcaatacat
       61 agcaccgcgc ctgctgcgcg ttttatgcgt ttggcgcgtt cggcggcggg aaatttgcct
      121 acttttcccg cgtcgggcgg gcggaacggg cggcacactg tctataaacc gcaataccgt
      181 ttacaatgac cgcctgtttc accacatacc cgaacgcaac aatgtttcaa cacacgggac
      241 ggcacatcaa gcaccgccct atgtgtcgtc ctgatttgga aggggttgca cccctcccga
      301 ataaagtctg atcctgccgc cccgaaggac ggatgtccga gtggcggggt ttcaaccgaa
      361 aaggaaatac aatgaaaatc aggccggggc ggcacaacgc gcccgacttt ccgcacgggg
      421 ccgccgtaac cataggcaat ttcgacggcg tgcacctcgg acacaaacac atcctccaaa
      481 aactccgcct cgaagccgac gcacgcggat tgcccgtcgt tgccgtcatt ttcgagccgc
      541 aacccaaaga attttttgcc gcccgcacag gcaaaacccc gccgtgccgt atcagccccc
      601 tgcgtaccaa actcgggctg ctcgaaaaaa ccggttgcgt cgatgccgcc tgggttttgc
      661 gtttcgatcg gaatttttcc gaaatatccg cgcaagcatt tatcgaccgc ctgctgcgtc
      721 aaaccttgaa tacgcgctat ttgctcgtcg gcgatgattt ccgtttcggt gcggggcggg
      781 aaggctgttt tgaacttttg gcacaacagc ccgatatgca aaccgagcgc acgccttccg
      841 tcatcgtcga agacatccgc accagcagta ccgccgtccg ccaagccctt tcagacggca
      901 accttgccta tgcgaagaaa cttttggggc acgactacgt tttggggggc agggtggtgc
      961 acggcagaaa actcggacgc accttaaacg ccccgaccgc caatatccgc ctgcccggcc
     1021 accgttatgc actcggcggc gtgttcgtcg tcgaggcgga cggcgcattc ggcacgcggc
     1081 gcggcgtggc gagcttcggc ttcaatccca ccgttgatgg cggctgttct caaaagcttg
     1141 aagtccacct gttcgacttt caaggcgatt tgtacggaca acggttgaac gtccgcttcc
     1201 tgcacaaact gcgcgacgag gaaaagtttg acggtatgga agaactgaaa aggcggattg
     1261 aagccgatat ggaagccgca aagtgttggt agaaaaacct tatacaaacc atccgattgg
     1321 gctacaatca gccttttaac tgttcggacg gcacaggggt ttcccgttgt gaaatactgt
     1381 ttgaggcgca atgccgtctg aaaccgaaat attgtaacaa tagagattaa aaaatgaccg
     1441 attacagtaa aaccgtcaac ctgctcgaaa gcccgtttcc gatgcgcggc aatcttgcca
     1501 agcgcgagcc tgcgtggctg aaaagctggt acgagcaaaa acgttaccaa aaactgcgcg
     1561 aaatcgccaa aggccgtccg aaattcatcc tgcacgacgg cccgccgtat gccaacggcg
     1621 acatccatat cggtcatgcc gtcaataaaa tccttaaaga cattattatc cgcagcaaaa
     1681 cccaagccgg ttttgacgcg ccttatgtac cgggttggga ctgccacggc ctgcccatcg
     1741 aagtgatggt ggaaaaactg cacggcaaag atatgcctaa agcccgtttc cgcgaattgt
     1801 gccgcgaata tgccgccgaa cagattgccc gtcagaaaaa agactttatc cgcttgggcg
     1861 tgttgggcga ttgggatcat ccttacctga ctatggattt caaaaccgaa gccgataccg
     1921 tgcgtatgct cggcgaaatc tacaaatccg gctatctcta ccgtggtgcg aaaccggttc
     1981 agttttgctt ggattgcgga tcttcgctgg cggaagcgga agtggaatac aaagacaaag
     2041 tatcgcctgc gattgacgtt gcctatccgt ttaaagacac tgccgcgctt gccgccgcat
     2101 tcggcttggc aggtatcgaa ggcaaagcgt ttgccgtcat ttggacgacc acgccttgga
     2161 ctctgcctgc gagccaggcc gtgtctgccg gcgcggacgt ggtgtatcaa ttaatcgata
     2221 cgcccaaagg caaattggtg ctggcgaaag atttggcgga aggcgctttg aaacgctacg
     2281 gcttttcaga cggcatcgcc atccttgccg aaaccaccgg cgacaagctg gaaaacctgc
     2341 acatgaatca tccgttcctc gaacgcgata ttcccatgct caacggcgaa cacgttacca
     2401 ccgatgccgg taccggcttg gtgcatactg cgcctgcgca cggtttggaa gactacgccg
     2461 tctgcaataa atacggcatc gagctttaca accctgtcaa cgccgaaggc aaatacataa
     2521 gcgaaacgcc tcgtgtcgca ggcatgagcg tttgggaggc gaatcccgtc atcctgcaat
     2581 ggccggaaga aaccggcaac ctcttggcaa gcagcaaaat cgaacacagc tacgcccact
     2641 gctggcgcca caaaaccccg ctgatttacc gagcgacagg tcagtggttt gtcggcatgg
     2701 acaaagccgg cagcgacggt aaaaccctgc gcgacaaagc catcaaagcc gtggacgaca
     2761 ccgaattctt cccgccatgg ggtcgtgcgc gtttggaatc catgattgaa ggccgtcctg
     2821 actgggtggt ttcacgccaa cgctattggg gcacgccgat gactttcttt gttcacaaag
     2881 aaacgggcga gctgcatccg aactctgccg aacttttgga aaaagtcgcg caacgcatcg
     2941 aagaaaaagg catcgaggct tggttctccc tcgataaaag cgaattatta agcgcggaag
     3001 attgcgaaca ttacgacaaa ctccccgata ccatggacgt atggttcgac tcaggctcga
     3061 cgcattattc cgttgtaaaa caacgcgaag aattggaatg gccggctgac ttgtacctcg
     3121 aaggcagcga ccaacaccgc ggctggttcc aatcctctat gctgaccggt tgcgcctcat
     3181 ccatgggacg cgcaccgtat aaacagctgc tgacccacgg tttcgtggtt gaccaaaacg
     3241 gccgcaaaat gtcgaaatcc atcggcaacg tcgtcgcgcc gcaggaagtg tataacgagt
     3301 tcggcgcgga catcctgcgc ctgtgggcgg catccaccga ttacagcggc gaattggcga
     3361 tttccaaaga aatcctcaaa cgcgtaaccg aaagctaccg ccgcatccgc aataccttga
     3421 gcttcctgtt tgccaacttg agcgatttca acccgattga agatgccgtg caacaggcgg
     3481 atatggtgga aatcgaccgc tacgccttgg tattggcgcg gcggctgcaa gagcgtttgg
     3541 caggcggtta ctatccgcgc tatgccttcc acttcgccgt gaaagacatt gtttctttct
     3601 gctcggaaga cttgggcgcg ttctacctcg acatcctgaa agaccgcctc tacaccacca
     3661 aagccgacag ccgcgcccgc cgcagcgcgc aaaccgccct gtaccacatc acgcgcagcc
     3721 tggttctctt gattgcaccg attttgtgct tcaccggcga agaagcgtgg gacatcatcg
     3781 gcggcggcga agaagacagc gtcctcttcc atacttggca cgagttcccg gccatcaacg
     3841 aaaaagccga agccgaactg gtgaaaaaat ggacggcaat ccgcgaagcg cgcgaagcag
     3901 taaccgccgc catcgagcct ttgcgcgccg acaaaaccgt cggttcgtcc ttgcaggcgg
     3961 aagccgaaat caccgcgccg gaagaaatgg ccggctatct gaatgctttg ggcgaagaat
     4021 tgcgctttgc cctgctggtg tctaaagcag aagtgaaagt cggtgatgaa cttgccgttg
     4081 ccgccaaagc cagcgacggc gaaaaatgcg aacgctgctg gcattacacc cgcgatgtgg
     4141 gcgcggttgc aggttatgaa accgtctgca aacgctgtgc ggagaatgtc ggcggagaag
     4201 gcgaaacgcg ccattacgcc tgataaagtt tgagcaaatg ccgtctgaaa ccgccaaaca
     4261 gtatttcaga cggcattttt tgtgccgcga tttgtcttca taatggcgga ggggtttcga
     4321 gattgcggtg ttgt
//
LOCUS       DAXDSD010000125         4192 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXDSD010000125 DAXDSD010000000
VERSION     DAXDSD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.125"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..428
                     /locus_tag="ACFB2Q_001932"
     CDS             <1..428
                     /locus_tag="ACFB2Q_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanine(37)-N(1))-methyltransferase"
                     /protein_id="HFC0918897.1"
                     /translation="ILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAV
                     LRLVPGILGDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLE
                     QSLRRTLERRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            443..808
                     /gene="rplS"
                     /locus_tag="ACFB2Q_001933"
     CDS             443..808
                     /gene="rplS"
                     /locus_tag="ACFB2Q_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC0918898.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            1129..1854
                     /locus_tag="ACFB2Q_001934"
     CDS             1129..1854
                     /locus_tag="ACFB2Q_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC0918899.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTSQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            1889..2764
                     /locus_tag="ACFB2Q_001935"
     CDS             1889..2764
                     /locus_tag="ACFB2Q_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC0918900.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVVFLILVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            2841..3755
                     /locus_tag="ACFB2Q_001936"
     CDS             2841..3755
                     /locus_tag="ACFB2Q_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFC0918901.1"
                     /translation="MKHLKLTLITALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
     gene            3975..>4192
                     /locus_tag="ACFB2Q_001937"
     CDS             3975..>4192
                     /locus_tag="ACFB2Q_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC0918902.1"
                     /translation="MKNILLVFVSFVPLCVRTDLPLNIEDIMTDKGKWKLETSLTYLN
                     SENSRAALASPVYIQTGSASFIPVPTEI"
BASE COUNT         1080 a         1264 c          913 g          935 t
ORIGIN      
        1 tgattctgct gtgcggacgc tatgagggaa tagacgaaag actgctgcaa agcagcgtcg
       61 atgaagaaat cagcatcgga gacttcgtcg tttccggcgg agagcttccc gccatgatgc
      121 tgatggatgc ggtattgagg ctcgtacccg gcatattggg cgacattcag tctgccgaac
      181 aggattcgtt ctcaagcggt attttggact gcccccacta caccaaaccc ttagaatttc
      241 aaggcatggc tgttccggaa gtattgcgct ccggaaatca tggcttgata gcggaatggc
      301 ggttggaaca atcgctgcgc cgcaccttgg agcgcagacc cgatcttttg gaaaagcgcg
      361 ttttaatccc aaaggaatcc cgcctcttga ataaaatcct acaagagcaa cgggaaatcc
      421 aatcataatt taggaaaaaa caatgaacct gattcaacag ctcgagcaag aagaaattgc
      481 ccgcctgaac aaagaaatcc ccgaattcgc accgggcgac accgtagtcg tatccgtacg
      541 cgtcgtggaa ggtacccgca gccgtctgca agcctacgaa ggcgtggtta tcgcccgtcg
      601 caaccgtggt ttgaacagca acttcatcgt ccgcaaaatc tccagcggcg aaggtgttga
      661 acgtactttc caactatatt cccctactgt tgagaaaatc gaagtcaaac gccgtggcga
      721 cgtacgccgt gccaaactgt actacctgcg cggtctgacc ggcaaagctg cacgcatcaa
      781 agaaaaactg cctgcacgca aaggttgatt caaaccgttt tcccccaatg ccgtctgaac
      841 gttcagacgg cattttttat tgccgcgatg ccggcaaggc aacgatatgc ctgaccctca
      901 tccaccttaa ttaacaatca ctcccgccta ttcaagcaga atttacagac taaacgttat
      961 acagtatcat atccgccata taacgataac cgctttgcca acttcccttt caaaactgca
     1021 aatcattatg acaccttccc tcttactgtc aggattgacc ttccgactct tcctcgccct
     1081 gattgccgta ttccttttat ggggtgttta cctttgggcg gtatcggtat gagcatcatc
     1141 gttgaaaacc tgacggtcag ctaccgccgc cgccccgccg tacaccacgt cgacattact
     1201 tttgaagaac atagtatgtg ggcggttttc ggtcccaacg gcgcaggaaa atctaccttt
     1261 ctcaaatcct taatgggatt gcagcctatc gatacgggca gcatccggct ggacggattg
     1321 accagtcaaa acattgccta tctcccccaa caatccgaca tcgaccgttc ccagcctatg
     1381 accgttttcg acttggcggc aatggggctg tggtatgaaa tcggtttttt caaagggata
     1441 aataccgctc aaaaacaacg cgttcacgaa gccttggagc gtgtcggtat gcggcagttt
     1501 gcccgccgcc aaatcgccca cctctcaaac ggacaattcc aacgcgtcct tttcgcccgg
     1561 atgctggttc aaaatgcaaa attcctgctt ttggacgaac ctttcaatgc tgttgatgca
     1621 cggacaacct acgagcttct cgacgtgttg caaaaatgcc attgcggcgg acacgctatc
     1681 attgccgttt tgcacgatta cgaacaagtc cgtgcctact ttcccaatac cctgctgctc
     1741 gcccgcgaaa aaattgcggc aggcgcaacc gagaccattc tgacagaatc ctacctcgcc
     1801 caagccagcg ccaaaatgca gcaacaggaa agccccgact ggtgcgcctc ataaatactg
     1861 cccgaaacca cgccccacac cagacaccat gaacctctac gacctgctcc ttgccccctt
     1921 tgcagaattc gacttcatgc actacgccct cgcatccgtc ttctgcctgt ccctcagcgc
     1981 cgcacccgtc ggcgtattcc tcgtcatgcg ccgtatgagc ctgataggcg acgcattgag
     2041 ccacgccgtc ctgcccggtg ccgccgtcgg ctacatgttt gccggcttga gcctgcccgc
     2101 tatgggtgtg ggcgggtttg ccgccggtat gctgatggcg ctgcttgccg gactcgtcag
     2161 ccgctttacc accctgaaag aagatgccaa ctttgccgcc ttttacctga gcagcctcgc
     2221 catcggcgta atcctcatca gcaaaaacgg cagcagcgtc gatttactcc acctcctttt
     2281 cggatctgtg cttgccgtcg atattcccgc actgcaactc atcgccgccg tctccggcct
     2341 cacgctcatt acccttgccg tcatctaccg ccccctggtg ctagaaagca tagaccccct
     2401 gttcctcaag tccgtcaacg gcaaaggcgg gctttggcac gtcgttttcc tcatcctcgt
     2461 cgttatgaac ctcgtatccg gcttccaagc tctcggcacc ctgatgtcgg tcggacttat
     2521 gatgctgccc gccattaccg cccgcttatg ggcaagaaat atggggacgc tcattctgtt
     2581 gtccgtcctc atcgcccttt tttgcggttt gatcgggctg ctcatttcct accacatcga
     2641 aatcccttcc ggccccgcca tcatcctctg ttgcagcgtc ctttatctct tttccgtcat
     2701 actcggcaaa gaaggcggca tcttgcccaa atggttcaaa aaccaccgcc accacaccac
     2761 ctgaccccgc aagccgcttg aacaaacagg cggcttgttt taccataatg aatgttatgt
     2821 tatatcacag aagaacagtt atgaaacacc tcaaactcac ccttattacc gcattgctgg
     2881 ccaccgccgc aactgccgca ccccttccgg ttgtaaccag tttcagcatt ttaggcgacg
     2941 tagccaaaca aatcggcggt gagcgcgtag ccgtacaaag cctcgtcgga gccaaccaag
     3001 atactcatgc ctatcacatg accagtggcg acattaaaaa aatccgcagt gcaaaactcg
     3061 tcctgctcaa cggcttggga cttgaagccg ccgacatcca acgcgccgtc aaacagagca
     3121 aagtatccta tgccgaagcg accaaaggca tccaacccct caaagccgaa gaagaaggcg
     3181 gacaccatca cgaccacgat catgaccacg aaggacacca ccacgaccac ggcgaatatg
     3241 acccccacgt ctggaacgac cctgttctta tgtccgacta tgcccaaaac gtcgctgaag
     3301 ccctgataaa ggccgatccc gaaggcaaag tttattatca acaacgcttg ggcaactacc
     3361 aaatgcagct taaaaaactg cacagcgacg cacaagccgc atttaatgcc gtccctgccg
     3421 ccaaacgcaa agtcctgacc gggcacgacg cattttccta catgggcaac cgctacaaca
     3481 tcagcttcat cgccccgcaa ggcgtgagca gcgaagccga gccgtccgcc aaacaagtcg
     3541 ccgccatcat ccggcaaatc aaacgcgaag gcatcaaagc cgtatttacc gaaaatatca
     3601 aagacacccg catggttgac cgcatcgcca aagaaaccgg cgtcaacgtc agcggcaaac
     3661 tgtattccga cgcactcggc aacgcgcccg cagacaccta catcggcatg taccgccaca
     3721 acgtcaaagc cttgaccaac gcgatgaagc aataaaaccg tttgattggt ttgaatttga
     3781 aaaggcaaaa ggccgtctga aaattaaata cgaggttttc agacggcctt ctcttgtgca
     3841 ataaagcagt aaacaaatat tgataagaaa aaaacaatca gtattgaaaa tatcgttaaa
     3901 aatattattt agacagatta catggaattt cattaacatc agtagacttt gatttcaatc
     3961 aagaaaacaa tgtcatgaaa aatattctct tagtatttgt tagctttgtg ccattatgtg
     4021 tccgcactga tctgccgctg aatatcgaag acataatgac cgacaaggga aaatggaaac
     4081 tggaaacttc ccttacctac ctgaacagcg aaaacagccg cgccgcactt gcctcaccgg
     4141 tttacattca gaccggctcc gcttccttta tccccgtccc gaccgaaatt ca
//
LOCUS       DAXDSD010000126         3937 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXDSD010000126 DAXDSD010000000
VERSION     DAXDSD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.126"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(376..3207)
                     /gene="lbpA"
                     /locus_tag="ACFB2Q_001938"
                     /gene_synonym="tbpA"
     CDS             complement(376..3207)
                     /gene="lbpA"
                     /locus_tag="ACFB2Q_001938"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC0918903.1"
                     /translation="MNKKHGFPLTLTALAIATAFPAYAAQAGAAALDAAQSQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSRGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTTEPFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3204..>3937)
                     /locus_tag="ACFB2Q_001939"
     CDS             complement(3204..>3937)
                     /locus_tag="ACFB2Q_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC0918904.1"
                     /translation="DEVSEDDNGEDEDEIVEEEADEAEEIEEEAEEEEPEEESPEEGN
                     GVSDGIPPAPEALKGRDIDLFLKGIRTAEADIPKTGTAHYTGTWEARIGEPIQWDNKA
                     DKAAKAEFDVDFGNKSISGTLTEQNGVEPAFRIENGVIEGNGFHATARTRDNGINLSG
                     NGSTNPQSFKADNLLVTGGFYGPQAAELGGTIFNKDGKSLGITEDIENEVENEADVGE
                     QLEPEVKPQFGVVFGAKKDNKEVEK"
BASE COUNT          800 a         1091 c          940 g         1106 t
ORIGIN      
        1 gccgccttgt cctgattttt gttaatccac tataatctca aaaatttatg ccattaaatc
       61 aataatttct gatgaatttt gaaaacttaa tcccgtcatt cccgcgcagg cgggaacccg
      121 gttcattgag cttcagctat ttagaataaa ttttgaaact ctaatcgcgt cattcccacg
      181 aaagtgggaa tccggttttt tgagtttcag tcatttccga taaattgcct tagcattgaa
      241 tgtccggatt cccgcctgcg cgggaatgac ggcggaaatc ttgtttatat tgaatcaaaa
      301 aaacctgcac cttaatcagt tggcggttta gtccgacttt tggggtgcag atcaagcttc
      361 cagacggcat ttcctttaaa acttcatttc gagcgcgagg ctgaagttcc tgcccggtgc
      421 ggcatacctt ccatagttgc tgtcgccgcc gtgccggttt gccgtgcttt ccgcagtctg
      481 gcgcaaggat tcccaagtaa cgtagcggta gttgccgata ttgtagatag ccgccctcaa
      541 ggtcagccgt tttttcagat tcagataagc ggaaacatct gccgttttcc aagaagacgt
      601 aacccttcct gccgaatatc gtttttgatc gcctgccaga taagcaagct cgtcagggtt
      661 tttccctttg gaataggtca gcataatgtt tgcgccccat ttcccctcgg gctggtcgta
      721 tccgaacccc aaaacataac gcgacggctg taccgcatcc aaagcatagc tgcggaggga
      781 caagtccggc cggttggata ccgatttcgg tttgatacgg ttgtacgcca atgtggtgta
      841 caggccttcg ggcagtttgc cgtatacgcc gttccagtcg attttcccca agatgttgat
      901 gccttgaagc gacatatttt gggcattgta ataatcgcgt atatcaatct ctgtcaatcg
      961 tcctgctgaa tccggcaatt tggttttttg atcggcaacg gcaatcatat cggtataacg
     1021 gttgcggaaa ctgctgattt ccaaaaagcc gaaatcgccc ttccactgca aaccgatttc
     1081 ccggttggct gccttttccg atttcagggc gggacgctgc cagcctttcg gataatcgtg
     1141 ataaatgtct atcccgaaaa gttcttggaa ggaaggcgtt ctgaagccgc tggaggcgcg
     1201 gtaagacacg gaaaaatgcc ggttcggttt gaacacgatg ccgctgttcc acgaacggtc
     1261 ggcataccgc ccgctgcgga cgagttcttc cgacgtggtg aagtttttcc ggtcgtacct
     1321 gccgcccaag ctgaaatcaa aatatttgcc gattgaaaaa cggtcgttca aagaaatgtg
     1381 gatattgctg ccgttgattt ttcttggcac gcatttgcgg gaacgcaggg tttcgatata
     1441 gccgcagacc gacccttcga cgacttcggg cttacccaaa cggtacttat cttgattctt
     1501 ttcatcgaat cccgtggatt ccgaaatcct tgccgcattg tgggaaagct gttctgggcg
     1561 ggagatcgct ttggaagcat cgtaaccgaa gcccaaagtc agatggtgtt tcgtccattt
     1621 gtttttcagc gatttctcaa acgaggcatt caaaacattg tgctgttcgc ggtagtggaa
     1681 acggtcgctg ctgtcgtagg aatacggttt gtccgccgac gcgcggcagg atttgtccac
     1741 ggcaggatac acggcgcaat tcagcttcag cgtgttgttg tcggttgcca cgccctgttt
     1801 gtcaaacgac aacaccgcct tatccgccca gttgtccgag tatttctcat tttcataacg
     1861 gtacagcaaa cccatacggc ggcggcggtg atgttcgtca ataaatttgg tgcgggaata
     1921 tttcaaacct atgcccttga ccaaattttt atcgcccttc cactcttcta tattcggcac
     1981 aaaatacaag ccgtcgcgga aatcgtcgcc gtcgtacacc ccgctcttgt ctctaaactt
     2041 ttccgcctcg tccgtaccgt aatactgttt ttccgtcata tcgcggatgt cgtaacgctg
     2101 tttggtatcc tcaaacacgc cgccgacata atgcctgccg ccgaagcggt agcccagctt
     2161 ggcaagccaa gagccgctgc ggtaatccat cggatcgggc aatatcctgc cgccgcccgt
     2221 gtaggcttgg gcggacaggt tttcgtggcg cgcctgcgcc tcccgcacct gcgcctcttc
     2281 ttcagcactt aaaggctgat tttgttcaat acgttctttt acccagcggt tgagctggcg
     2341 gttcaaatat atcccgtagc ccgccaattt tgccacgggc ttgagtgtat tctcgccctc
     2401 tactaagaaa aatggctctg ttgtcttgcg tttaatatcg tatgtctggc ggaacgcgtc
     2461 caaacggtct atgccgtatt ccaccccgtc cgcaatatcg ccgtgcgggc gcgtttcccg
     2521 cccttggcgt tcggttcgga ttagcagccc ttcccaaccg tctttgctga accccgcgcc
     2581 gagcgacttc ataaattggc ggtttttact gccgtaagcg gtttttgcct gtatcccccg
     2641 gcttttgccg tctgaaatca ggtctgccgc ctctttggtg cggaaggcga ccgcgccgcc
     2701 gagtgcgccg ctgccgtgat cggacgaacc ggcgcctttg tcgatttcca ccgtgctgat
     2761 gttttcatat tcgatttcgt tgattgcgcc gctgccgccg cgtccgccgt atccgctcaa
     2821 cgatccctgc acggtaaacg cctgtatttg ggcaacgccg tcaaccgaaa ccgccacacg
     2881 gtttttatct acgccgcgta tcgagtagcc gccgctcgcg ccgttgccct gttcgacaac
     2941 cgccacgccc ggatcgtagc gcgtcaggtc gcggataccg agtacctgtt ctttgttcaa
     3001 cgtttccgac gttttgacga ttttgcccaa acctgtcgcc tctttcgatc gccgtcccac
     3061 tttggcggca cggacggtaa cctctttcaa tgattgactt tgcgccgcat caagtgccgc
     3121 cgcccccgct tgggcagcat aagccggaaa agcggttgca atggccaacg ccgtcaaagt
     3181 cagcggaaaa ccgtgtttct tattcatttt tccacctctt tattatcttt cttcgcaccg
     3241 aataccacgc cgaattgggg tttaacttca ggttctaact gttcgccaac atcagcttca
     3301 ttttcaactt cattttcaat atcttcagtt ataccaagag atttcccatc cttattgaaa
     3361 atagtgccgc ccaattccgc cgcctgcggg ccgtaaaagc cgcccgttac aagaagattg
     3421 tcggctttga aactttgagg attagtcgaa ccatttcccg aaagattgat gccgttatcc
     3481 cgagtgcgtg ctgttgcgtg gaaaccgttg ccctcaatca cgccgttttc aatacggaaa
     3541 gcaggttcta caccgttttg ctccgtcagc gttccggaaa tcgatttatt gccgaaatca
     3601 acgtcaaatt ctgcttttgc cgctttatcc gccttattat cccattgaat gggttcgccg
     3661 atacgcgctt cccaagtgcc ggtataatgc gccgttccgg tttttggaat gtcggcttcc
     3721 gccgtgcgga tacctttcag gaaaaggtcg atgtccctgc ctttaagggc ttccggggcg
     3781 ggcgggatgc cgtctgaaac accgttgcct tcttccggcg attcttcttc gggttcttcc
     3841 tcttcagctt cttcttcgat ttcttcagct tcatcagctt cttcttcgac gatttcgtct
     3901 tcgtcttcgc cgttatcatc ttcggaaact tcgtctt
//
LOCUS       DAXDSD010000127         3861 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXDSD010000127 DAXDSD010000000
VERSION     DAXDSD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.127"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            201..1802
                     /locus_tag="ACFB2Q_001940"
     CDS             201..1802
                     /locus_tag="ACFB2Q_001940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC0918905.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKEKPDEPIEFVNMDIDGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNADLFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHVIGEDKCMIYTQDISQKSSNLLRLNLSLNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFVQKIAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            1834..1950
                     /locus_tag="ACFB2Q_001941"
     CDS             1834..1950
                     /locus_tag="ACFB2Q_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC0918906.1"
                     /translation="MPSESLSDGILFGLENTYAVLSPKERIQKTKATGIHRK"
     gene            complement(2113..3450)
                     /gene="ribBA"
                     /locus_tag="ACFB2Q_001942"
     CDS             complement(2113..3450)
                     /gene="ribBA"
                     /locus_tag="ACFB2Q_001942"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC0918907.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(3663..3791)
                     /locus_tag="ACFB2Q_001943"
                     /pseudo
     CDS             complement(3663..3791)
                     /locus_tag="ACFB2Q_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
BASE COUNT          979 a          833 c         1051 g          998 t
ORIGIN      
        1 acgcagatgg ctctcgtgta gaaatttcta ctgatgtaca gctgaaacat ttgttatatg
       61 gtattgatga acgttattac acaacagcat taggaaaaga gaaaaggttg gcaaatagtg
      121 ttcaacctat ttaatctgcc agcaaaccaa ctaatgctaa acaaaattta agcgacgcta
      181 gcaacaaacc gagacattcc atgaccgaac aacactttac cgaacaaatc aaatccctaa
      241 tcgacagctt aaaaaccatc tgcgccaact atggtttggg caatgacggc aacgaattta
      301 aaatcatcag tcaggcgttt ttgtataaat tcctaaacga caaatacgat tttgaagtta
      361 aaaaaattcg caaagaaaag cccgacgagc cgatcgagtt cgtcaatatg gatattgatg
      421 gaaaaacagc ggttttaaag cccgaacatt ccatcaagta tttatcagag cggcaaaacg
      481 gcgcggattt tgccaaactc tttgacgata cgctcaccga tattgccgcc tgtaatgcgg
      541 atttgttttc ggtgaaaacc gaaggcgggg cgaaaatcgt gttgttcgag cgtatcagtc
      601 agtacattac cgacgaaggc cgacgcgacg atttttgccg cgcgctgatt tccaagctgg
      661 cgggtttcag cttcgaggcg atttttgcgc aaaaattcga ctttttcgcc acgatttttg
      721 aatacctgat taaagactac aacagtaatt ccggcggcaa atatgccgaa tactacacgc
      781 cgcacgcggt tgcgcggatt atggcggata ttttggtgcc ggaggaggtg cgcgggcaaa
      841 tccgcagcgt ggacgtttac gacccgtcgg ccggttcggg aacgctgttg atgaatgtcg
      901 cccatgtcat cggcgaagat aaatgcatga tttatactca ggatatttcg cagaaatcgt
      961 ccaatttgtt gcggctgaat ctgagtttga acaatctggt gcattcgctg aataatgtgg
     1021 ttcagggcaa tacgattttg tcgcccgccc acaaagacgc ttcaggctgt ctgaaaaaat
     1081 ttgatttcat tgtttccaat ccgccgttta aattggattt cagcgatttc cgcgatcggc
     1141 tggaaagcga tgaaaaccac gaacgctttt ttgctggtat cccgaaaatc aagccaacaa
     1201 agaaagagaa gatggaaatc tatcagcttt ttatccagca tattctgttc agcctgaaag
     1261 aaaacggcaa ggcagcgatt gtattaccga ccggctttat cacggcaaaa tcgggcatcg
     1321 ataaaaaaat tcgcgaatat ctggtggaaa acaaaatgct cgccggcgtg gtgtctatgc
     1381 cttccaatat tttcgctacg acgggaacca atgtttctat cttgtttatt gataaagtca
     1441 ataaggataa agttgtatta atcgatgctt ccggtttagg tgaaaaaatt agtattaatg
     1501 acaaccaaaa aactgtactt tcccacgaag aagaacaaaa aatctgccat accttcacgc
     1561 acaaacaggc agtagaagat ttcagcgtgg tggtcggcta tgatgaaatc aaagcgaaga
     1621 atcacagctt gtcggcgggg cagtattttg aggtaaaaat tgattatgtg gatatatccg
     1681 ccgacgaatt tgtgcaaaaa atagcgggat tttcagcgga tttggataaa cttttcgccg
     1741 aatctgccga attggaaaag gagattaagg atagattggc gatgttgaaa ttcaattcat
     1801 aaataccgat gaatgaaacc ggtcaaactt aaaatgccgt ctgaaagcct ttcagacggc
     1861 attttgtttg gtttggaaaa tacttatgcc gttctttccc cgaaagagag aatccaaaaa
     1921 accaaagcca caggaattca tcggaaatga cacaaaaacc cgacgaaccg gattcccgcc
     1981 tgcgcgggaa tgacgaatta gaagttaccc gaaatccgaa aaaagcaaaa tccaacccaa
     2041 cagaccgggt tttcgtttgt acggaaataa tgcaataaat gaagcaaata taaagcattt
     2101 gaatttacta tattattttt ccgcttcttc aaagccgacg acttcgaggc cgaagccggt
     2161 gaggccggtg aaagatgacg gttggccgag gacgcgcagt tttttgacgt ggaggccggc
     2221 gaggatttgc gcgccgatgc cgtagctttt gctgtcccat ttgtaggctt ggtttgcgcc
     2281 tttgggtagg gtgcggtcga gcagggatgc gccgtcttcg gtgtggtgca ggaggatgac
     2341 gacgccgctt tcggcctgtt ggacgcgctc aagggctttg ggcagcgacc aggaatggcg
     2401 cggattggct tggatgaagt ccatcacgct gaagggttcg tggacgcgga cgagggtttc
     2461 ggtgtcggcg gagggtgtgc ctttgacgag ggcgaggtgg gtttcgccgg agagtttgtc
     2521 gacgtaaacg tgttgttgga actcgcccca cggagtttgt acgggcgcgt cgcccatatc
     2581 ttcgagcagg ctttcggtgc ggctgcggta ttcgatgagg tcggcaatcg tgccgatttt
     2641 gagcttgtgt tcttcggcga atttcatcag ttcgggcata cgcgccatcg tgccgtcgtc
     2701 gttgatgatt tcgcagatga cggcggcggg aatcagcccg ttcatttgcg ccaagtcgac
     2761 gccggcttcg gtgtgtccgg cacggacgag tacgccgcct ttttgggcgc gaagcgggaa
     2821 gatatgaccg ggttggacga tgtctgcggg tttggcggtc ggggaaacgg cggtttgaat
     2881 agtcagggcg cggtcggcgg cggaaatgcc ggtggtaatg ccgtgcgcgg cttcgatgga
     2941 aacggtaaag ttggtaccgt attgcgcgcc gtttttttgg gtcatcatcg gcagaccgag
     3001 tttttcgacc attgcacctt ccataggcag gcagactaag ccgcgcgcgt gtttgatcat
     3061 aaagttgacg gcttcggggg taacgaattg cgctgccatc agcaggtcgc cttcgttttc
     3121 gcggtcttcg gcatcggtga tgatgaccat tttaccggct ttgatgtcgg ctaggatttc
     3181 ggggatgggg gaaatatggg acatggtgtg ttttcctgtg tgtccgggcg gtcggccggt
     3241 gtgttcggta tcgagccaga aggcgggcat ttttgccgcc tgttcgattt tgcgggcttt
     3301 tttctcgccg aaggatttgt ttttcaaaag ggcggagagt tcgcctgtgt tgagggcaac
     3361 ggcttcggca aaacggtttt gcagtccgcc gtagtgttcc gctatccatt gccgcaggtt
     3421 gcggcggcgc aagccggctg tgtcggtcat cgttttatcc tttgtagaaa tcggatgtag
     3481 cggattgtaa gcgcgtattt actaaaaagc aaatttgttt ttcgccaaat cggattattt
     3541 gccttttggt aaacagatgc cgtttgaagc cggattcgtg gcttcagacg gcattgccgc
     3601 aaccccgccg gcggggcggc tgtttcttcc cgcacgcttc tgattatggg gtttccggag
     3661 ggttattccg aaaaaaaatt ctgataaaaa gccttgattg ccgggtacgg cataggcggt
     3721 acgagcaggc gcaataccgg caatcctatg atgaatgccg ccttcccgat aaaaaccgga
     3781 cggattgccc tttactttct acaagtaaca gggctttttt ttgcccgttt ttgaggattc
     3841 gtaccatgga agataaggaa g
//
LOCUS       DAXDSD010000128         3845 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXDSD010000128 DAXDSD010000000
VERSION     DAXDSD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.128"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            150..2177
                     /gene="uvrB"
                     /locus_tag="ACFB2Q_001944"
     CDS             150..2177
                     /gene="uvrB"
                     /locus_tag="ACFB2Q_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC0918908.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDALRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(2355..3113)
                     /gene="cah"
                     /locus_tag="ACFB2Q_001945"
     CDS             complement(2355..3113)
                     /gene="cah"
                     /locus_tag="ACFB2Q_001945"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFC0918909.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
     gene            complement(3522..>3845)
                     /locus_tag="ACFB2Q_001946"
     CDS             complement(3522..>3845)
                     /locus_tag="ACFB2Q_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918910.1"
                     /translation="ISIVLYILLLQINLKMLEKRIDFLVENIDKYYQQYGSYPNNFDF
                     ISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTILVMGFSSKILFSSHNKIKEFN
                     SNKYD"
BASE COUNT         1035 a          999 c          891 g          920 t
ORIGIN      
        1 accggtttaa atttaatcta ctatacttgg gtagcgattg ggtttcagac ggcatacatt
       61 caggatttca ccgccttata ccgtctgaaa gacgtttctg acccatccct actacacgct
      121 ttgtccgcaa acgtatcaca ggcaagatta tggaagtcat ccgataccca aattccccct
      181 tcaaactcca ccaacccttc ccgcccgcag gcgaccagcc caccgccatt gcgggcctgc
      241 tcgaagggct ttcagacggc cttgcctacc aaaccctgct cggcgtaacc ggttcgggca
      301 aaacctacac gatggcgaac gtcatcgcgc aaagcggcag acccgccatc attatggcgc
      361 acaacaaaac ccttgccgcc cagctttatg ccgaaatgcg cgagtttttc cccgaaaacg
      421 cagtggaata tttcgtgtcg tattacgact attaccagcc cgaagcctat gtgcccagcc
      481 gcgatttgtt catcgaaaaa gacagcgcga tcaacgaaca catcgagcag atgcgccttt
      541 ccgccaccaa aaacctgatg acgcgcgacg acgtgattat cgtcgccacc gtgtccgcca
      601 tttacggtat cggcgacccg accgagtatc aacaaatggt gctgtccgta aaagaaggcg
      661 acaccatcga gcagcgcgac atcatcgcca cgctcgtttc catgcagtac gaacgcggcg
      721 atttggactt caaacgcggc agcttccgcg tgcgcggcga cgtgattgac gtgtaccccg
      781 ccgaaagctc cgaaaacgcc ttgcgtatca gcctgtttga tgacgaaatc gaccgcctcg
      841 atatgttcga cccgctttca ggcagcctgc accagcgtgt cggacgctac accgtcttcc
      901 cgtccagcca ctacgttacc ccgcgcgaca ccgtattgcg cgcctgcgaa tccatcaaag
      961 aagaattgcg cgaacgcatc gaatttttcg ctcgcgaaca acgccccgtc gagcaacaac
     1021 gcatcgaaca gcgcacccgt ttcgacctcg aaatgctcta cgaaatgggc ttctgcaaag
     1081 gcattgaaaa ctactcccgc cacttctccg gtaaaaaaga aggcgaaccg ccgcccacgc
     1141 tgatggacta cctgcccgac aacgccatca tgttcatcga cgaaagccac gttaccgtta
     1201 cccaaatcgg cggcatgtac aaaggcgacg cattgcgcaa gcaaaacctc gtggactacg
     1261 gcttccgcct gccttccgcc cgcgacaacc gcccgctcaa attccacgaa tttgaaaaag
     1321 tcatgccgca aaccgtcttc gtttccgcca cccccgcgaa atacgaagaa gaacacgccg
     1381 gacaagtcgt cgaacaagtc gtccgcccca cagggctggt cgacccccaa atcatcatcc
     1441 gccccgtcgc cacccaagtc gacgatttaa tgagcgaaat caacgaccgt attcaaaaag
     1501 gcgaacgtgt gctcgttacc acccttacca aacgcatggc ggagcaactc accgactatt
     1561 acagcgaact cggcatcaaa gtgcgctacc tgcacagcga catcgacacc gtcgaacgcg
     1621 ttgaaatcat tagagatttg cggctcggcc tgtttgacgt actcgttggc atcaacctgt
     1681 tgcgcgaagg cctcgacatc cccgaagtct ccctcgtcgc catcctcgat gccgacaaag
     1741 aaggcttcct gcgctcccac cgcagcctga ttcaaaccat aggccgcgcc gcgcgcaacg
     1801 tgaacggcgt cgccatcctg tacgccgaca aaattaccga ctccatgaaa gccgccgtcg
     1861 acgaaaccga acgccgccgc gaaaaacaga ttaaattcaa cgaagaacac ggtatcgtgc
     1921 cgcagcagat taaaaaacag gtcaaagaca tcatcgacgg cgtgtaccac gaagaagaca
     1981 gtggcaaagg ccgtagacaa ggcaaaaaca aggtcaaagt cggcgaaatc cacaacgaag
     2041 aagacgcgat taaagaaatc gccaaactgg aaaaagccat gcagcaggcg gctagggatt
     2101 tacagtttga agaagctgct gtgttgaggg ataggattag gaatattaaa gagaatttgt
     2161 tgtttggggc ggagtgattt tgaaatagta atcgcagcct gaatacatag cgtgtgcgcc
     2221 actggcgcgc gctcattgtg ttatcaggaa tccttgaaat catgtacgca gcttgcatca
     2281 cacatcctag ctatacataa acaacaaggc cgtctgaatt ttcagacggc cttttccgtt
     2341 tacaacttta ctacttattc aataactaca cgtgcattca gaggctgtac ggggcggttg
     2401 ttttccgaac cgacggcgcg ggtgaatttt tccgcttgcg cttggtcgat gtggtcataa
     2461 gttttcaaca ccaaccatga tacgccctct gtgcacggcg gcgtggtcag cgaaccggca
     2521 aagcggtagt atttcaaccg tttcggcagt agggtggatg cgtcgaacgg ttggttgagt
     2581 ttcacttttc ctgcggtcat cggcatgacg ttccagatgg aagacaggcg gccgttggtt
     2641 ttgccggctt catacagcac ggctaatact aaaggctgtt tgttttcgtc taagtggacg
     2701 aagtgagctt ccatcgggaa agtgcggcct ttgatttggt tttcgctcgg cacgtggaag
     2761 tggaactgtt tcagggtata ggtgcggccg ttcacggtca gggtattgcc gccttcggga
     2821 taattgacct gaatggtgtg gccgttgttt tccacgtcaa ccatactcgg tttgtaattg
     2881 actttgatgg cgggcagttt gccggaaacg gtttcggtaa tgtttaccgg agattggttt
     2941 ttgccggtgg agcacaaacg gaattcttct gacagattgc cccagctttc gggagagtcg
     3001 tgtccggtat agccccaatg ggtgtgattg ccgtgtgcgg cggcggagaa agcggtacag
     3061 gccaacagca ggacagcggt caggcggggt aaagtgcggg ggaaacgagg cattgtaaga
     3121 tcctttctat taaggagtag aaaaaaggtt gcagtgtgta tgtaaatatt ttatttttta
     3181 ttaaatgata tctatattaa gtgcttaagt caaacttccc ggtctgaaaa ccattagagc
     3241 aatgaaatta aagtgaattt atttttctat aagtcctgct ttatttggat aaccatgagg
     3301 catggctacc tttatccgga aaaatattaa tagccgcaac aaagatattt gaatccgatt
     3361 caaatatcac cagcaagcgt agctcggata cttgtatccg acaaagcctt taacatttcc
     3421 atcgttgcaa tccattgcag caattaccaa aatgtcggat tcgagaatcc gatctacgga
     3481 aaactaaaga ttttatgcgg gctacggctt gcttgttgta tttaatcata tttatttgaa
     3541 ttgaattctt ttattttatt gtgagatgaa aatagtattt tcgatgagaa acccatgact
     3601 aaaatagtat agtctttatc attctttacg aagtaacaat taccatatcc tgcaatattt
     3661 ttatcccaaa aatcacaata tgattcagtg gtaaaatcag ttttagttga tataaaatca
     3721 aaattatttg ggtaagatcc atattgctgg taatatttat ctatattttc tactaagaaa
     3781 tcaatccttt tttctaacat tttaagatta atttgcaata ataaaatgta taaaactata
     3841 ctaat
//
LOCUS       DAXDSD010000129         3751 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXDSD010000129 DAXDSD010000000
VERSION     DAXDSD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.129"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(161..934)
                     /locus_tag="ACFB2Q_001947"
     CDS             complement(161..934)
                     /locus_tag="ACFB2Q_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC0918911.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
     gene            complement(1034..1657)
                     /gene="rsmG"
                     /locus_tag="ACFB2Q_001948"
     CDS             complement(1034..1657)
                     /gene="rsmG"
                     /locus_tag="ACFB2Q_001948"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC0918912.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            complement(1752..2231)
                     /locus_tag="ACFB2Q_001949"
     CDS             complement(1752..2231)
                     /locus_tag="ACFB2Q_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918913.1"
                     /translation="MFVNEKYPYATLFAGLVFLTLPFALAVHDAFACAFGRAGLLVSV
                     SDGGFGRRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAVAWIFKLLLGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            2338..2413
                     /locus_tag="ACFB2Q_001950"
     tRNA            2338..2413
                     /locus_tag="ACFB2Q_001950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2371..2373,aa:Thr,seq:cgt)
     gene            2483..3610
                     /locus_tag="ACFB2Q_001951"
     CDS             2483..3610
                     /locus_tag="ACFB2Q_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC0918914.1"
                     /translation="MNSSQRKRLSGRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTIGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
BASE COUNT          850 a         1305 c          897 g          699 t
ORIGIN      
        1 ccttagggaa tcgttccctt tgagccgggg cggggcaacg ccgtaccggt ttttgttaat
       61 ccgctataaa tgacgatatt aagtattttt attttaatcc gccatattaa cgcacccggc
      121 caaacagcat aaaggcacgg gcagcccgat ttggattgac ctatttcccc gacaccctcg
      181 ccgccagctc gtccgccaag gcaagatacg ccttggcacc ctttgcctgc gcgtcgtaag
      241 ccatcaccgg cataccgtgg ctcggcgctt ctgcaaggcg gatattgcgc gggatggcgg
      301 tttcaaaaag caaatccccg aaatggctgc gcaactgttc gctgacttcg gcaaccagcc
      361 tgctgcggct gtcgtacatc gtgcgcacga tgcccgtgat gtccaaatcg ggattgaccg
      421 cctgacggat tttgcgcacg gtcgcaatca aatcggaaat cccttccagc gcgtaatatt
      481 cgcacaacat cggcacaatc acgccgcccg ccgccaccaa gccgttaagc gtcaacagcg
      541 tcagcgaagg cgggcagtcg atcaggataa agtcgtaatc ttccgccact gccttgagcg
      601 cgtttttcaa acgcacttcc cgggcgattt cctgcaccag ctcgatttcc gcgccggcaa
      661 gcgcgcggtt cgcacccagc acagcgtatc cgccctcttt gctgcgtacc gccgccgact
      721 gcacgtccgc atcgcccaat aagacctgat aaacgccgga ctgcaaaccc gccttgtcga
      781 tgccgctgcc cgtcgtcgca ttgccctgcg gatccaaatc gaccaccagc acgcgtttgc
      841 cgcgcgatgc cagcgaagcc gccaaattta ccgtcgtcgt cgttttgccc acaccgccct
      901 tctgattggc gatggcaagg atgttcgcac tcatgtcccg cccgtgtccc gtctgatgtt
      961 gaaaaaaaga aaaacagtgc ggaattttac ctttatccgc acaaaaaagg tattcatgcc
     1021 gtctgaagcg cgctcaacgc ttgctcagga tgacgatatg gcgttccgca tccaagcccg
     1081 gcacgtcgag cctttggact ttttcgacgc acacatcctg cggcaggcgg ccgatttccc
     1141 cctgcggata tacgcccttc atcgccgccc agtagccgcc gtctttcaac aggtgcgccg
     1201 tccacgacac aaaatccgcc agttccgcaa acgcacggct ggtaaccacg tcggcacgca
     1261 cgtccgaaac cgcctccacg cgccccgaca ccacgcgcac attgtccagc cccaactcga
     1321 tagccgcctg ccgcaaaaaa gccgttttct tcgtattcgc atccaacaag gttatctgca
     1381 catccggacg gcacaccgcc gccggaatgc cgggctggcc gccgcccgaa ccgacatcca
     1441 gcatcgtctg tgcgccttcg atatagggca gcagcgtcag gctgtccaaa agatgatgga
     1501 caatcatttt ttcctcgtcg cgcagggcgg tcagattgta ggttttgttc cactttttca
     1561 acaaatccac ataggccaaa agcctgtcct gcgccgtttc cgaaatatcc ggccccatcg
     1621 cggcaatgcc tgcacgcagg cgttctttac gttccatatc tgcttcctgt ccatattcag
     1681 gcgtaatgtt agcggaaaaa catgccgtct gaaaccgcgt atcgggtttc agacggcatt
     1741 tccgttccgt cctatttttt cctgtggaac accacaggct gcaccggcgc gcgcgtcagg
     1801 cgtttcggca gcgtcagcat agacaccagc cacacggcaa acgcatattc cgacaccgcc
     1861 tccccgctca gcccgcccaa ggcggcactg cccaacaaca gcttgaaaat ccaagccacc
     1921 gccgcgcaag ccgccacgcc cgacagaaac agcgcgtaac gcgaatgccg ccgcatcatc
     1981 tttttgtacg ccagtttcgt caggcccgcc gacacaacca cattcaaaaa cgcaaacaca
     2041 ccgaacacaa accaaacagt gccgtcccaa ccgccacgcc ggccgaatcc gccgtccgac
     2101 accgacacca gcagccccgc ccgtccgaac gcacatgcaa aggcatcatg caccgccaac
     2161 gcaaacggca gcgtcaaaaa caccagcccc gcaaacaggg tcgcataagg atatttctcg
     2221 ttcacaaaca tcgccgtccc ccctcttccg aagcaggccg cattataccg cgcaccgcct
     2281 tgccgcccgc ccgaaaagtt gcacaaacaa ccgtttatat atatcataac gaaaaacgcc
     2341 ggtgtagctc agtcggtaga gcagcgcatt cgtaacgcga aggtcggggg ttcgattccc
     2401 ttctccggca ccaataccaa gcacagaccc tccctcggga agcctgtgct ttttcacatt
     2461 tccgcttcag acggcatccg atatgaactc ctcgcaacgc aaacgccttt ccggccgctg
     2521 gctcaactcc tacgaacgct accgccaccg ccgcctcata catgccgtgc ggctcggcgg
     2581 agccgtcctg ttcgccaccg cactcgcccg gctactccac ctccaacacg gcgaatggat
     2641 agggatgacc gtcttcgtcg tcctcggcat gctccagttc caaggcgcga tttactccaa
     2701 ggcggtggaa cgtatgctcg gtacggtcat cgggctgggc gcgggtttgg gcgttttatg
     2761 gctgaaccag cattatttcc acggcaacct cctcttctac ctgaccatcg gcacggcaag
     2821 cgcactggcc ggctgggcgg cggtcggcaa aaacggctac gtccctatgc tggcggggct
     2881 gacgatgtgc atgctcatcg gcgacaacgg cagcgaatgg ctcgacagcg gcctgatgcg
     2941 cgcgatgaac gtcctcatcg gcgccgccat cgccattgcc gccgccaaac tgctgccgct
     3001 gaaatccaca ctgatgtggc gtttcatgct tgccgacaac ctggccgact gcagcaaaat
     3061 gattgccgaa atcagcaacg gcaggcgtat gacgcgcgaa cgtttggagc agaatatggt
     3121 caaaatgcgc caaatcaacg cacgcatggt caaaagccgc agccacctcg ccgccacatc
     3181 gggcgaaagc cgcatcagcc cctccatgat ggaagccatg cagcacgccc accgcaaaat
     3241 cgtcaacacc accgagctgc tcctgaccac cgccgccaag ctgcaatctc ccaaactcaa
     3301 cggcagcgaa atccggctgc tcgaccgcca cttcacactg ctccaaaccg acctgcaaca
     3361 aaccgccgcc ctcatcaacg gcagacacgc ccgccgcatc cgcatcgaca ccgccatcaa
     3421 ccccgaactg gaagccctcg ccgaacacct ccactaccaa tggcagggct tcctctggct
     3481 cagcaccaat atgcgtcagg aaatttccgc cctcgtcatc ctgctgcaac gcacccgccg
     3541 caaatggctg gatgcccacg aacgccaaca cctgcgccaa agcctgcttg aaacacggga
     3601 acacggctga cggccggaca cgatgccgtc tgaaacgcct gccgcttcag acggcatcgg
     3661 tccgataaac accctgacgg ttttgctata atctgccgca aaaccccaag gaaaaaccat
     3721 gaacattatc gatgccatca tcaatttggc t
//
LOCUS       DAXDSD010000130         3683 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXDSD010000130 DAXDSD010000000
VERSION     DAXDSD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.130"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..507
                     /locus_tag="ACFB2Q_001952"
     CDS             <1..507
                     /locus_tag="ACFB2Q_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC0918915.1"
                     /translation="VNLDMSNPTVLRMGLPLYIASLRRGAIYKVWQFVEDALRAVVPA
                     DSFEPTAQKLKLFKVGAATILFYEDQNVVKGLQEQFPAYAANFPVWADQANAMVQYAV
                     WTTLAAVGAGANLQHYNPLPDVAIAKAWNIPENWLLRAQMVIGGIEGAAGEKVFEPVA
                     ERLKVFGA"
     gene            complement(562..1017)
                     /locus_tag="ACFB2Q_001953"
     CDS             complement(562..1017)
                     /locus_tag="ACFB2Q_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HFC0918916.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHSAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1273..2643)
                     /gene="ffh"
                     /locus_tag="ACFB2Q_001954"
     CDS             complement(1273..2643)
                     /gene="ffh"
                     /locus_tag="ACFB2Q_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC0918917.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMK
                     GMFPGL"
     gene            2865..3671
                     /locus_tag="ACFB2Q_001955"
     CDS             2865..3671
                     /locus_tag="ACFB2Q_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC0918918.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT          807 a          913 c          987 g          976 t
ORIGIN      
        1 gtcaacttgg atatgtccaa tcctacggtg ttacgcatgg gattaccctt atatattgcg
       61 tccctaagaa ggggcgcaat atataaggtg tggcaatttg tcgaagacgc gctgcgtgcc
      121 gtcgtgcctg ccgacagttt tgaaccgacc gcgcaaaaat tgaagctgtt taaggtgggc
      181 gcggcaacca ttttgtttta tgaagatcaa aatgtcgtca aaggtttgca ggagcagttc
      241 cctgcttatg ccgccaactt tcccgtttgg gcggaccagg cgaacgctat ggtacagtat
      301 gccgtctgga cgacacttgc cgcggtcggt gcaggtgcaa atctgcaaca ttacaacccc
      361 ttgcccgatg tggcgattgc taaagcgtgg aatattcccg aaaactggct gttgcgcgcg
      421 caaatggtta tcggcggtat tgaaggggcg gcaggtgaaa aggtctttga acccgttgcg
      481 gaacgtttga aagtgttcgg cgcataattt cgcggtcaaa aaaatgccgt ctgaaccccg
      541 ttcagacggc atttttcagt atcaggcggc gagtttttcg cattctttga tacagtcgag
      601 gcaggactcg taacaggctt tgcattccgc atggtgggcg gagtgttctt tacaggcttt
      661 ggcacactgt ttgcacgctt cgaggcacac ttttgccgcg tcgcgtgtca gaggggaatt
      721 ttgtgcggca aggtcgtgca ccgcgccgca taaggcaagc atctggcgca cggcaaccgc
      781 acagtcggac atagacgtgt cgccctgagt gagcagggac aggcagtggg tcaggcaaac
      841 ctgtccggca tccagacagt gtgcggcagt ttggcggacg gcggtgtagg cggatgcggc
      901 ggcaggctgc atatcgtgat gatggtggta gtcggcgtgt ccgtgcgcac gcgcgaaaga
      961 ggcggcggaa gccaaagaga cggcagcggc gctgcccaaa aattgacgac ggttcatgat
     1021 tttttccttt atgtttcaac atgcgggtcc ggcataaggt acacacttac catactgccc
     1081 tggccggatc ggtacgattt tctgaataac tgcttgattt tgcaggtgtg aaattgtcgc
     1141 acgagcaggc atgttttagc tattcggaaa ttcgaggaag ggtgcatcaa cctgttttga
     1201 aatgatgcgc agacatggta acagacaaaa agccgtctga aaatcgtttt agacggcttt
     1261 tgttttcaaa cattaaagac cgggaaacat tcccttcatt cccttcatgc ctttggccaa
     1321 gcgcatcagt ttgcccaaac cgttgccgct gaacatcttc atcatctgct gcatttgttc
     1381 aaactgtttg agcaatttgt tcacttcctg cacggtcgtg cccgcgccca tcgcaatacg
     1441 gcgtttgcgg ccggctttga gcagggcagg gttggcgcgt tctttagggg tcatcgagtt
     1501 gatgatggct tctactttgc ccatcgcttt ttcagccgta ccttcgggga tttgtttcga
     1561 tatttgaccc agttcgcccg gcattttcga catcaggttt tccaaaccgc ccatattgcg
     1621 catttgctgg atttgttctt taaaatcatt gaggtcaaag cctttgcctt tttgcagctt
     1681 tttcgccatt ttggcggcgg cttcttcatc aatacctttt tgaacgtctt caatcagggt
     1741 caatacgtcg cccatgccga ggatacggct ggcaagacgg tcggggtgga aaggttcgag
     1801 gccgttgatt ttctcgccga cgccgataaa tttaatcggt ttgccggtta catggcgtac
     1861 ggacaatgcc gcaccgccgc gcgagtcgcc gtccatcttg gtcaatacga cgccgttcag
     1921 cggcagggct tcattaaatg cctgagcggt atttaccgca tcctgaccca gcatggcatc
     1981 gataacgaac aaagtttcaa ccgggttgac caccgcatga agcgctttga tttcgttcat
     2041 catctcttca tcgattgcca aacggccggc ggtatcgacc atcaatacat cgtaaaaatg
     2101 ttttttggcg taatcaacgg cggcagttgc aatctcaacc ggtttttggt tggtatcgga
     2161 tgggaaaaaa tctacgccga cctgttcggc caacaggcgc agctgttcaa tcgcggcagg
     2221 gcggtaaacg tcggcggata ccaccaaaac ttttttcttc tgatcgtttt tcagcaggcg
     2281 ggcgagtttg ccgacggttg ttgtcttacc ggcaccctgc aaaccggcca tcaaaatgat
     2341 ggcaggcggt gaaaccgata aatccagtgt tttgttttcc ttgcccatca gttcgactaa
     2401 ggcttggttg accacgccga taaaggcttg gtcaggcgtc aggctgcccg ccacttcctg
     2461 accgagggcc ttttctttga cgttgttgac gaactctttg acgacaggca gggcgacatc
     2521 cgcctcaagc agggcgagtc ggacttcgcg caaggcttct ttaatattgt cttcggtcag
     2581 tttggcctgc ccccggatgt ttttgaagac attgctgaag cggccggtta aattgtctaa
     2641 catacttggt ccttggtctg aagtaagaat agcttgcccc atcaggggca ttctttgtta
     2701 aaataaaatc aaaataattt gatgcggctt gtgtgccgga cagcatatcg gcaaatccgt
     2761 caaggcttgc ccgaaacggg gattttacaa ttccaacgtt aaaagttcca atatttcata
     2821 agcggccgca tacggcgcaa cagtatagat agagaaagtc caccatgccg acagttttca
     2881 tctttttgac ggcggtttac gcaggattgg gtgcatttgc atggcactgc caacagcagg
     2941 ggcgcggccg ggattacccg tggaagacgg aattgccggt tttgggcgcg gcattgaccg
     3001 tccacggcgc ggcactgctg atgccggtca ttcaagacaa aatcatcatt atgggcttcg
     3061 ggtattccgg cagcctgatt gtttggatga tgctgtttat ttattttgcc ggcagctttt
     3121 tttatccgct gcgcggagtg cagttgctgc tgtatccttg cgccgcactg atgctgctgt
     3181 caggtttggt tttcccggga aaattctcgg gatatgaaat taccgacttt ccctttatgc
     3241 tgcatatcgg cacatcactg cttgcatacg ggctgttcgg catcgcaaca ttattgtccg
     3301 ttttgagctt gctgctgcac cggagcctgc accgcagaaa cttctccaag ctcgcaggat
     3361 tcctgccgtc gctgctcagt ttggaaaaac tcatgttcca ggccatgtgg gcaggtttca
     3421 tcctgctgac ctattccgtc gtcagcggca cattctttgc cgaagccgta ttcggcaaac
     3481 ccatgacatt tacccataaa accgtattcg gcatattgtc atggctgatt tacggaggac
     3541 tgctgctcaa acacagcatg accgcatggc gcggcaaaaa agccgccgtg tggaccatca
     3601 tcggatttgt cagccttatg attgcctata tgggcagcaa gttcgtattg gaaatcattc
     3661 tgaaaagata agaagagcca aca
//
LOCUS       DAXDSD010000131         3644 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXDSD010000131 DAXDSD010000000
VERSION     DAXDSD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.131"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            170..931
                     /locus_tag="ACFB2Q_001956"
     CDS             170..931
                     /locus_tag="ACFB2Q_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918919.1"
                     /translation="MEMQLDKQTLTVVVRNSEPVELSVFAQSMMSLADDYESMCGSAG
                     SHARLYIKEIRQGSIIAELAPLLPLAGTLFEGAGQILAYAKNFIEITDWLMGKGKEPA
                     GVTDSQIRNIANIMQPAASDKNGSIEINVNDNNGSVVNNITYNYFAANTVQNQARRIL
                     GERAEASESGDYGQMVMYFVQAAPTKETNQAVIEGIYSRPVKILIPEHIKREMFAEPY
                     PFEKYYIVDVSVQTARGKPRLYKVTGYHGVVDGDD"
     gene            complement(1030..1431)
                     /locus_tag="ACFB2Q_001957"
     CDS             complement(1030..1431)
                     /locus_tag="ACFB2Q_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFC0918920.1"
                     /translation="MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAY
                     MHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVMIEIDPAKISRQQIRFNVSWPQY
                     LLDRVDEYTSANHETRSGFLAKAALLTMNQA"
     gene            complement(1533..1715)
                     /locus_tag="ACFB2Q_001958"
     CDS             complement(1533..1715)
                     /locus_tag="ACFB2Q_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFC0918921.1"
                     /translation="MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKD
                     LPTGTVKNIYKQAGLK"
     gene            complement(1885..2703)
                     /locus_tag="ACFB2Q_001959"
     CDS             complement(1885..2703)
                     /locus_tag="ACFB2Q_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HFC0918922.1"
                     /translation="MNQYAMRFAVIRFMPYVQTREFANIGIIITHPKSGYFDFKIEQR
                     YSRLSRFFRRFDSSVYKAATRAFAEELQRIGKLAAYSAPDQIRAMLDHLTRPREALIM
                     AAQPGVTLAPDRGQELNRLFDYFVARSFAKSQPEAELTRQIQAMLKPLQTAYPFKEST
                     IGDPSGFHASIPLVQKAENGEIRKIIKPIYFGQKDPADIYYKSDKWIAGIKRLRRGGY
                     IDRSEILFAYEPPERPDKAQEKALLDVLGDLEEQGIRLADNRSEGKIIRNFACG"
     gene            complement(2700..3398)
                     /locus_tag="ACFB2Q_001960"
     CDS             complement(2700..3398)
                     /locus_tag="ACFB2Q_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HFC0918923.1"
                     /translation="MDRAEQGVTSPFICMAENGLEYFVKGLHATRASQINEWIGGNMA
                     QALGLPVAPFELLEVGEELYEELPAKMKEIGKGICFGSLAQKGCALLEPADIPRIDTV
                     MQRQIAAFDWFVRNEDRTIGNPNLLYRNCNNSLIVIDHNCAFDTGFNPNNFLQNHIFS
                     SAFRQILEDWVLREEMELWLKSALPAYRKACDNLPPEWAWANEERDLPAAYNRSRTDE
                     TVRRIDNGTLWSIS"
BASE COUNT          872 a          831 c          954 g          987 t
ORIGIN      
        1 gtactaaagt acttatatat tgtaaagtac aatagtacta ttattttata tttagtatat
       61 tagtatttga ttttcaaaag aatttatttt tgagattccg caggcgcaaa aaaaccgccc
      121 gatatttcgg gcggcgtatt aaaatcagat gaaattaagg aggcttatta tggaaatgca
      181 attggacaaa cagacattga cagtggttgt cagaaacagc gagccggtgg aattgtcggt
      241 atttgcgcag tctatgatga gccttgccga tgattacgaa tccatgtgcg gcagcgcggg
      301 cagtcatgcg cggctgtata ttaaggaaat caggcagggc agcattattg ccgagcttgc
      361 acccctcttg cctttggcgg gtacgctttt tgaaggcgcg gggcagatat tggcgtacgc
      421 caagaatttt attgagatta ccgattggct gatgggtaaa ggcaaagaac ccgcaggggt
      481 aacggacagc caaatccgca atattgcaaa tatcatgcag cctgccgcat cggataaaaa
      541 cgggtcgatt gaaatcaacg tgaacgacaa caacggctcc gtcgtcaata atattaccta
      601 caactatttc gcggcaaaca cggtacaaaa tcaggcaagg cggatactgg gcgaacgggc
      661 ggaagcgtcc gaatcagggg attacgggca gatggtgatg tatttcgtac aagccgcgcc
      721 gacaaaggaa accaatcagg cggttattga agggatatac agccgtccgg tcaaaatcct
      781 gattcccgaa cacatcaagc gcgaaatgtt tgcagagcct tatccatttg aaaaatatta
      841 tattgttgac gtaagcgtac agacggcgcg cggcaagccc aggctctaca aagttaccgg
      901 gtatcacggg gtagtcgatg gggacgattg aaaaccgcct tttcgggcgg ctttgtcggt
      961 tttgtgttgt tttcaggttc ggcggggcgt gaaaaaagcc cgcgtgtgcg ggcgggttga
     1021 acgggctggc tatgcctgat tcatggtaag cagggcggct ttggctaaaa aaccgctacg
     1081 ggtttcatga tttgcggatg tatattcatc cactctatca agaaggtatt gcggccagct
     1141 gacgttgaat cggatttgct gccgactgat ttttgcaggg tcgatttcaa tcattaccca
     1201 tgttgcccca tgataatcag gttcttggct caaatcggca atgctgctta cggcaaggtt
     1261 tttgaaccct ccgtcctcaa tcataccgtc aatatgcata taggcggcag aacgggcgtt
     1321 ggcgacggct tcttcaacgg tgtcgccgca agagaaacag ccgggaaggt cggggatggt
     1381 tacgccatat gccgaatgtt cgtctttgtg cagggcggca gggataaaca tttgatttcc
     1441 tttaattatg ttgttcaggt ttatgggcgc gtgagataac ccgttgattg tttgattaga
     1501 aggggggcag cgggaaaccc cgctgcctgc cgttacttca aaccggcttg cttatagata
     1561 ttttttacag tacctgtcgg caagtctttt ttcgggtgcg gcacggttac acggcctttt
     1621 tttgttgggt gcttatattg cgaatggctc ccggattgtg caactttata ccaaccgtct
     1681 tgtttgagta gggcaataac gtctaggcta ttcaattatc actcctaatc ttaatgcatg
     1741 gcacgcctgc caatgtgcgt aataatacac actaattttt attttgtcaa tttttatgtg
     1801 tatttgtgtg taaaaatatc tttcggcagt agttgcaggg aagggtgtaa aaagcccgca
     1861 taacgcgggc ggtcttgagg gctgtcagcc gcaggcaaag tttctgatga ttttgccttc
     1921 gcttcggtta tcggcaagcc gtatgccctg ttcctccagg tcgcccaata cgtcaagcaa
     1981 tgccttttct tgtgccttgt cgggacgctc cggcggttcg taggcaaaca atatttcaga
     2041 gcggtcgatg tatccgccgc gccgcaaccg ttttatgccg gcaatccact tatcagactt
     2101 gtaatagatg tcggcagggt ctttctgtcc gaaatagata ggcttgatga ttttccgtat
     2161 ttcgccgttt tccgcctttt gcaccaaggg gatggaggcg tgaaagcctg acgggtcgcc
     2221 gattgtgctt tctttgaagg ggtatgccgt ctgaagcggc tttaacattg cctgtatttg
     2281 tcgggtaagt tctgcttcgg gttggctttt agcaaagctg cgggcgacaa aataatcaaa
     2341 caggcggttc agttcctgcc ccctgtcggg ggcgagggtt acgcccggtt gggcggccat
     2401 aatcagggct tcgcgcgggc gggtcaaatg gtcgagcatg gcgcgtatct gatcgggcgc
     2461 ggagtatgcc gccaatttcc caatccgctg taattcttct gcaaaggcac gggtcgccgc
     2521 tttatagacg gacgaatcga agcggcggaa aaaacggctc aatctgctgt aacgctgttc
     2581 gattttaaag tcgaagtagc cgcttttggg atgggttatg atgatgccga tgttggcaaa
     2641 ttcgcgcgtt tggacatagg gcataaaccg tatgacggca aaacgcatgg catattggtt
     2701 catgagatgc tccaaagtgt tccgttatca atgcggcgga cggtttcgtc ggtgcggctg
     2761 cggttatagg cggcgggcag gtcgcgctct tcgtttgccc atgcccactc cggcggcaga
     2821 ttatcacacg cttttctgta ggctggcaat gcgcttttca gccataattc catctcttcc
     2881 cgaagcaccc aatcttccaa aatctgtcta aatgccgatg agaaaatatg attttgcaga
     2941 aagttatttg gattgaagcc ggtgtcgaag gcgcagttat gatcaataac aatcaaggaa
     3001 ttgttgcaat ttctatacaa aaggttaggg ttgccgattg ttctgtcttc attacggaca
     3061 aaccaatcga aagcggcgat ttgccgctgc atgactgtat cgattcgcgg gatgtccgca
     3121 ggctccagta acgcacagcc tttttgcgcc agtgagccga aacagatgcc tttgccgatt
     3181 tctttcatct ttgcgggcaa ttcttcgtac agctcttctc cgacttccaa cagctcaaac
     3241 ggagcaacgg gcaagcccaa agcctgcgcc atgtttccgc ctatccattc gtttatttgg
     3301 cttgcacggg tggcgtgcag ccctttaacg aaatattcca gaccattctc cgccatacag
     3361 atgaacggcg aggtaacacc ttgctccgca cgatccatta ttgtctgtat ctgcaacatt
     3421 tcttttcttt atcctatgtt tcaacacaca ggacgacaca taaagcaccg ccctatgtgt
     3481 tgccctgatt cggaaggggt tacgcccctc ccaaacagag tctgatcctg ccgccctaaa
     3541 gggcggggtt tcaaccgaaa aggaaatacg atgaaattta cactttgtgt aaatctgtaa
     3601 atccgacttt ttgagatttc cccctagagg aatttgtcaa tcca
//
LOCUS       DAXDSD010000132         3639 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXDSD010000132 DAXDSD010000000
VERSION     DAXDSD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.132"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(305..1147)
                     /locus_tag="ACFB2Q_001961"
     CDS             complement(305..1147)
                     /locus_tag="ACFB2Q_001961"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC0918924.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETGVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            1382..2302
                     /gene="pyrB"
                     /locus_tag="ACFB2Q_001962"
     CDS             1382..2302
                     /gene="pyrB"
                     /locus_tag="ACFB2Q_001962"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC0918925.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            2312..2770
                     /gene="pyrI"
                     /locus_tag="ACFB2Q_001963"
     CDS             2312..2770
                     /gene="pyrI"
                     /locus_tag="ACFB2Q_001963"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC0918926.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            2832..3497
                     /locus_tag="ACFB2Q_001964"
                     /pseudo
     CDS             2832..3497
                     /locus_tag="ACFB2Q_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          815 a          943 c         1112 g          769 t
ORIGIN      
        1 tgttaatcca ctataaatag cgagcatcga ttcacggtgc gctttagtgc aaagcgtaca
       61 acgcgagcct gaaccaccag ccgcaatagg aaaagaaagc cgatagagtg caatgcttgc
      121 caacgcgcaa gcgagcttgc aagaacgctt ggctcaacga gagcaggcaa gacagaaagc
      181 aggataggag aggcaacgca aaggtctcgg gctttgattt cgccgtaaac ctgagtgccg
      241 ccttgtccgg acggggtggt tcaggcggcg ggtgccgaca gggtgcagat gaggaggagt
      301 attttcattt ggggtcgcaa cggaagtggt aggcgcagat ttcaaaaccg tttttgaaat
      361 acaggcggtg cgcgtcggta cggtcgtggt tgacgtggac gttaaggtgg attttggtaa
      421 cccctgtttc cgcgctgatt ttgcggactt cttccaaaag gcgcgaggcg tagcctttgc
      481 ggcggctttg cggcagggta acgatgtcgt cgatgtggat gtggcggccg ccggcgaggg
      541 tgcaggcttc gcggaagccg cagacagcga cggcgttgtg tttgccttct tcaaaaatgc
      601 ccagcaggcg gtagccttgg gggcgttggg ttttgttgat ctgttcggta aagcggccga
      661 tgtcggtcag ggcggaacgc aaaacactca aggctgcaaa ggcggtggcg gtgtcgtccg
      721 cgccgatttc gcgtaaaacg taggatgcgc ccgaggcggt ctgttcctgt gttttctcgg
      781 cggcgtgttt ttcttcgatt gcctgtgcca gcaggacgcg ttcgttggca gggtcgtttt
      841 gtccgccctg ttcgcgttct tcgagcaggg ctttgcagtc gatgacgcgc aggtcgttgt
      901 cggcggcaaa gtccatcagg aagcggaaca tctgcggatt gtcggtttcc agttttttat
      961 cgacggcgac gcagcggatg ttgtccacca gcatggggcg cacccatttc gaataggaga
     1021 ggcggttgtc tttggtgaac gaggacagga tgccgcacag gcgttccgcc cagtcgccgg
     1081 ggcggaaaat cttgccggaa ctcgttgtgc cgtggatgac gacttcgtag gggttgcaga
     1141 ttaacatggc ggcttcctga aaagaaatgt ctggcgcgat tataccttat gcttatgcgg
     1201 gcgtgtttgg atatgccgtc tgaaaaatac aggattcgtg cggtaaaact ttgtggaggc
     1261 aaatgtgcga taatacgcgc cgtgtcgccg cttttgcgaa gctgttccgc aaacatacgg
     1321 gcggcgtgga cgtataaccg gatgcccgcc tgacgcgggt tttttacgga aggggggcaa
     1381 aatgcctaat ccgctttaca gacagcacat catctccatt tcggatttgt cgcgcgaaca
     1441 gttggaatgc ctgcttcaga cggcattgaa gctgaaggcg catccgcgcg gcgatttgtt
     1501 ggaaggcaaa ctcatcggtt cgtgcttttt cgagccgtcc acgcgcacga ggctgtcgtt
     1561 tgaaacggcg gtgcagcgtt tgggcggtaa ggtcatcggc ttttcggacg gcgcgaatac
     1621 cagtgccaaa aaaggcgaaa cgcttgccga taccgcccgt atcatttccg gctacaccga
     1681 cgcgattgtc caacgccatc ccaaagacgg cgcggcgcgc gtggcggcgg agttttcgcg
     1741 cgtccccgtc atcaacgccg gcgacggcac gaaccagcac cccagccaaa ccttgttgga
     1801 cctggtaacg atttatgaaa cgcaggggcg tttggacaag cttaaaatcg ccatggcggg
     1861 cgacttgaaa tacgggcgca ccgtgcattc gctgtgtcag gcgttgaaac gctggggctg
     1921 cgaatttgcc ttcgtctcgc cgcccagcct cgcgatgccc gactacatta ccgaagagtt
     1981 ggaagaagcc gactgccgat accgcgccct cggcagtttg gaagaggcgg cggaatgggc
     2041 ggacatcctg tatatgaccc gcgtccagcg cgaacgtttc gacgaacagg aatttgccaa
     2101 aatccaaggc aaattcaacc tcgaagcgtc tatgctcgcc cgcgccaaac cgaacctgcg
     2161 cgtgctgcac cccctgccgc gcacggacga aatccacccc gatgtcgatg ccgcgccgca
     2221 cgcctattat ttcgagcagg cgaccaacgg cgtttatgcg cgtatggcga tattgtcgct
     2281 ggtgttgaac gaagaagtgt gaggaaccga tatggaagcg caaaaactca gtgtcgaagc
     2341 catcgaaaaa ggcacggtta tcgaccatat tcccgccggc agggggctga ccatcctgcg
     2401 ccagttcaaa cttttgcact acggcaacgc ggtaaccgtg ggcttcaacc tgcccagcaa
     2461 aacccagggc agcaaagaca tcatcaaaat caaaggcgtg tgcttggacg ataaagccgc
     2521 cgaccgcctc gccctgttcg cccccgaagc ggtggtcaac accatcgaca atttcaaggt
     2581 cgtacagaag cggcatttga ccctgcccga cgaaatcgcc gaagtgttcc gctgcccgaa
     2641 tcccaattgc gccggccacg gcgagccggt caaaagccgg ttttatgtta agaaacacaa
     2701 cgggcagacg cggctgaaat gccactactg cgaaaaaacc tacaaccggg attcggtggc
     2761 ggaagcctga cggattcccg agcccggcgg cggaattttg cctgccgcct gttttgccaa
     2821 tctgaaatgg aatgatgatg cacgcttctg tccaaagtcg tttcgcaccg atactttatg
     2881 ttttgatttt ctttgccggt tttttgaccg cgcaaatctg gttcaatcag aaagcctata
     2941 ctgaagagct gcctccgctt ctgtccgcat tgtccgccgt cgcgctggtg tggctggcgt
     3001 gggcgttcgt gtcggtgcgt tcaaaggcta aggcagaaaa gttctaccgc gaaaaaatga
     3061 tacagaacga aagcataccc ccgtcctgca cgcttctttg caacacttgg aacacaagcc
     3121 gcaaatgctc gccctgctgg tcaaaaacca cggcaaaggc atggcggaac aggtcaggtt
     3181 caaggcggaa gtgctgcccg acgacgaaga cgcgcgcacg attgccgccg agttggcaaa
     3241 aatggatatg ttcgcattgg ggacggacgc ggtcgcctcg ggcgaaacct atgggcgcgt
     3301 gttcgccgat attttcgagt tgtcggcggc tttggaaggg cgcgcgttca aagggatact
     3361 gaaactgacg gcggaatata aaaaacatct tcggcgatgc ctgccgttcg gaaacggcgt
     3421 tggatttggg cgcgctcaat caggcgttga gggaaatctc gaaaacgccg gaaaagccta
     3481 agcagatatt ttattgaagg tgtcccgatg tatggggagc gcacgggtgg cgcggcggca
     3541 aagcggcaag attgggtttg gatacccggt tggttttata gtagattaac aaaaatcagg
     3601 acaaggcggc gggccgcaga cagtacaaat agtacggaa
//
LOCUS       DAXDSD010000133         3552 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXDSD010000133 DAXDSD010000000
VERSION     DAXDSD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.133"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            98..3297
                     /gene="nalP"
                     /locus_tag="ACFB2Q_001965"
                     /pseudo
     CDS             98..3297
                     /gene="nalP"
                     /locus_tag="ACFB2Q_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
     gene            3349..>3552
                     /locus_tag="ACFB2Q_001966"
     CDS             3349..>3552
                     /locus_tag="ACFB2Q_001966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918927.1"
                     /translation="MKRKPYSNCLLLLFQDFFQRFGINQTYSGLTKIRTRRRAAGSTN
                     GTEPIRPVLHHLGEPFPLSRGGAT"
BASE COUNT          952 a          971 c          967 g          662 t
ORIGIN      
        1 gatataattc aaaatataaa aatgggttac atctggacat tacgaaattt ttattccttc
       61 gcctgaattc tattgtcaga ttcaaagaga cctcatcatg cgaaccacct caaccttccc
      121 tacaaaaact ttcaaaccgg ctgccatggc gttagctgtt gcaacaacac tttctgcctg
      181 cttaggcggc ggcggaggcg gcacttctgc tcccgacttt aatgcaggcg gcaccggtat
      241 cggcagcaac agcagggcaa cgatagcgga atcagcagca gtatcttacg ccggtataaa
      301 aaacgaaatg tgcaaagaca gaagcatgct ctgtgccggt cgggatgacg ttgcggttac
      361 agacagggat gccaaaatca aagcccccga atctgcatac cagagacttt tcaaacccaa
      421 atgaccaata ttaagaatat gatcaacctc aaacctgcaa ttgaagcagg ctatacagga
      481 cgcggggtag aggtaggtat cgtcgataca ggcgaatccg tcggcagcat atcctttccc
      541 gaactgtatg gcagaaaaga acacggctat aacgaaaatt acaaaaacaa attacaaaaa
      601 ctatacggcg tatatgcgga aggaagcgcc tgaagacaga ggcggtaaag acattgaagc
      661 ttctttggac aatgaggccg ttatagagac tgaagcaaag ccgacggata tccgccatgt
      721 aaaagaaatc ggacacatcg atgtggtctc ccatattatt ggcgggcgtt ccgtggacgg
      781 cagacctgca ggcggtattg cgcccgatgc gacgctacac ataatgaata cgcatgatgg
      841 aaccaagaac gagaaataat gtctgcagcc atccgcaatg catgggtcaa gctgggcgaa
      901 cgtggcgtgc gcatcgtcaa taacagtttt ggaacaacat cgagggcagg cactgccgac
      961 catttccaaa tagccaattc ggaggagcaa taccgccaaa cgttgctcga ctattccggc
     1021 ggtgataaaa cagatgaggg tatccgcctg atgcaacaga gcgattaggg caacctgtcc
     1081 taccacatcc gtaataaaaa catccttttc atcttttcga caggcaatga cacacaagct
     1141 cagcccaaca cactgaccct attgccattt tatgaaaaga cgctcaaaaa ggcattatca
     1201 cagtcgcagg cgtagaccgc agtggagaaa agttcaatgg ctccaaccat tgcggaatta
     1261 ctgccatgtg gcgcctgtcg gcatcctatg aagcaaccgt ccatttcacc cgtacaagac
     1321 cgattcaagt tgccggaaca tccttttccg cacccatcgt aaccggcacg gcggctctgc
     1381 tgcagagata cccgtggatg agcaacgaca acctgcgtac cacgctgctg acgacggctc
     1441 aggacatcgg tgcagtcgga gtggacagca agttcggctg ggggctgctg gatgcgggta
     1501 aggccatgaa cggacccgcg tcctttccgt tcggcgactt taccgccgat acgaaaggta
     1561 catccgatat tgcctactcc ttccgtaacg acatttcagg cacgggcggt ctgatcaaaa
     1621 aaggcggcag ccaactgcaa ctgcacggca acaacaccta tacgggcaaa accgttatcg
     1681 aaggcggttc tctggtgtta tcggatatgc acgtcaaaac caaaggtgca ctgatttata
     1741 acggggcggc atccggcggc agcctgaaca gccacggcat tgtctatctg gcagataccg
     1801 accaatccgg cgcaaacgaa accgtacaca tcaaaaggcg gtctgcagct gggcgacaaa
     1861 ggtacgctgt acacacgttt gggcaaactg ctgaaagtgg acggtacggc gattaccgac
     1921 ggcaagctgt acatgtcggc acgcggcaag ggggcaggct atctcaacag taccggacga
     1981 catgttccct tcctgagtgt cgccaaaatc gggcaggatt attctttctt caaaaatatc
     2041 gaaaccgacg gcggtctgct ggcttccctc gacagcgtcg aaaaaacagc gggcagtgaa
     2101 ggcgacacgc cgtcctatta tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg
     2161 gcacattccg cgcccgccgg tctgaaacac accgtagaac agggcggcag caatctggaa
     2221 aacctgatgg tcgagctgga tgcctccgaa tcatccgcaa cacccgagac ggttgaaact
     2281 gcggtcgccg accgcacaga tatgccgggc atccgcctac ggcgcacaac tttccgcgca
     2341 gcggcagccg tacagcatgc gaataccgcc gacggcgtac gcatcttcaa cagtctcgcc
     2401 gctaccgtct atgccgacag tgccgccgcc catgccgata tgcagggacg ccgcctgaaa
     2461 gccgtatcgg acgggttgga ccacaacggt acgggtctgc gcgtcatcgc gcaaacccaa
     2521 caggacggtg gaacgtggga acagggcggt gtcgaaggca aaatgcgcgg cagtacccaa
     2581 actatcggca ttgccgcgaa aaccggcgaa aatacgacag cagccgccac actgggcata
     2641 ggacgcagca catggagcga aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt
     2701 gcaggcatac ggcacgatgt gggcgatatc ggctatctca aaggcctgtt ctcctacgga
     2761 cgctacaaaa acagcatcag ccgcagcacc ggtgcggatg aatatgcgga aggcagcgtc
     2821 aacggcacgc tgatgcagct gggcgcactg ggtggtgtca acgttccgtt tgccgcaacg
     2881 ggagatttga cggttgaagg cggtctgcgc cacgacctgc tcaaacagga tgcattcgcc
     2941 gaaaaaggca gtgctttggg ctggagcggc aacagcctca ctgaaggcac actggtcgga
     3001 ctcgcgggtc tgaagctgtc gcaacccttg agcgataaag ccgtcctgtc tgcgacggcg
     3061 ggcgtggaac gcgacctgaa cggacgcgac tacgcggtaa cgggcggctt taccggcgcg
     3121 gctgcagcaa ccggcaagac gggtgcacgc aatatgccgc acacccgccg ggttgccggt
     3181 ctgggggtgg atgtcgaatt cggcaacggc tggaacggct tggcacgtta cagctacacc
     3241 ggttccaaac agtacggcaa ccacagcgga caaatcggcg taggctaccg gttctgacgg
     3301 acagaaaaca gacagccgca aagatcacgg tctttgcggc tgtttcttat gaaaagaaaa
     3361 ccctattcca attgcctgct tctattgttt caagacttct tccaaagatt cggcatcaat
     3421 cagacgtata gcggattaac aaaaatcagg acaaggcggc gggccgcagg cagtacgaat
     3481 ggtacggaac cgatccgccc ggtgcttcat caccttgggg aaccgttccc tttgagccgg
     3541 ggcggggcaa cg
//
LOCUS       DAXDSD010000134         3507 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXDSD010000134 DAXDSD010000000
VERSION     DAXDSD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.134"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            13..1809
                     /locus_tag="ACFB2Q_001967"
     CDS             13..1809
                     /locus_tag="ACFB2Q_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC0918928.1"
                     /translation="MQYKPLLLALMLVFSAPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEIAATKA
                     QISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKISKDARKLLEQKG
                     NEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMS
                     NLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYS
                     TAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAGSK
                     IGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            1936..3411
                     /locus_tag="ACFB2Q_001968"
     CDS             1936..3411
                     /locus_tag="ACFB2Q_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC0918929.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMAVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
BASE COUNT         1031 a          812 c         1025 g          639 t
ORIGIN      
        1 gaaagccgaa ccatgcaata caaacccctc ctgcttgccc tgatgcttgt cttttccgcc
       61 cccgccgttg ccgcccacga cgcggcacac aaccgttccg ccgaagtgaa aaaacaggcg
      121 aagaacaaaa aagaacagcc cgaagcggcg gaaggcaaaa aagaaaaagg caaaaatgcc
      181 gcagtgaaag ataaaaaaac aggcggcaaa gaggcggcaa aagagttcaa aaaaaccgcc
      241 aaaaaccgca aagaagcaga gaaggaggcg acatccaggc agtctgcgcg caaaggacgc
      301 gaaggggata aggaatcgaa ggcggaacac aaaaaggcac atggcaagcc cgtgtccgga
      361 tccaaagaaa aaaacgcaaa aacacagcct gaaaacaaac aaggcaaaaa aggggcaaaa
      421 ggacagggca atccgcgcaa gggcggcaag gcggaaaaag acactgtttc tgcaaataaa
      481 aaagcccgtt ccgacaagaa cggcaaagca gtgaaacagg acaaaaaata cagggaagag
      541 aaaaatgcca aaaccgattc cgacgaattg aaagccgccg ttgccgctgc cactaatgat
      601 gtcgaaaaca aaaaagccct gctcaaacaa agcgaaggaa tgctgcttca tgtcagcaat
      661 tccctcaaac agcttcagga agagcgtatc cgccaagaac gtatccgcca agagcgtatc
      721 cgtcaggcgc gcggcaacct tgcttccgtc aaccgcaaac agcgcgaggc ttgggacaag
      781 ttccaaaaac tcaataccga gctgaaccgt ttgaaaacgg aaatcgccgc tacgaaagcg
      841 cagatttccc gtttcgtatc ggggaactat aaaaacagcc agccgaatgc ggttgccctg
      901 ttcctgaaaa acgccgaacc gggtcagaaa aaccgctttt tgcgttatac gcgttacgta
      961 aacgcctcca atcgggaagt tgtcaaggat ttggaaaaac agcagaaggc tttggcggta
     1021 caagagcaga aaatcaacaa tgagcttgcc cgtttgaaga aaattcaggc aaacgtgcaa
     1081 tccctgctga aaaaacaggg tgtaaccgat gcggcggaac agacggaaag ccgcagacag
     1141 aatgccaaaa tctccaaaga tgcccgaaaa ctgctggaac agaaagggaa cgagcagcag
     1201 ctgaacaagc tcttgagcaa tttggagaag aaaaaggccg aacaccgcat tcaggatgcg
     1261 gaagcaaaaa gaaaattggc tgaagccaga ctggcggcag ccgaaaaagc cagaaaagaa
     1321 gcggcgcagc agaaggctga agcgcgacgt gcggaaatgt ccaacctgac cgccgaagac
     1381 aggaacatcc aagcgccttc ggttatgggt atcggcagtg ccgacggttt cagccgcatg
     1441 cagggacgtt tgaaaaaacc ggttgacggt gtgccgaccg ggcttttcgg gcagaaccgg
     1501 agcggcggcg atgtttggaa aggcgtgttc tattccactg cgcctgcaac ggttgaaagc
     1561 attgcgccgg gaacggtaag ctatgcggac gagttggacg gctacggcaa agtggtcgtg
     1621 atcgatcacg gcgagaacta catcagcatc tatgccggtt tgagcgaaat ttccgccggc
     1681 aagggttata cggtcgcggc aggaagcaaa atcggcacga gcgggtcgct gccggacggg
     1741 gaagaggggc tttacctgca aatacgttat caaggtcagg tgttgaaccc ttcgggctgg
     1801 atacgttgat cggattgccg aaacggcagg ctttatgcaa atcgtgccga tatggtgcgc
     1861 tgttccgtga agtttgccgt ttgccgtacc tgtgttttcc ggtttttgga aatcaagaga
     1921 gaaagttaaa gaagaatgtc gaaacctgtt tttaagaaaa tcgcacttta tactttgggt
     1981 gcaatcagcg gcgtggccgt aagtctggcg gtgcagggtt ttgccgccga gaaggacggg
     2041 cgggataacg aagtcctgcc ggtgcaatcc atccgtacga tggcggaggt ttacggtcag
     2101 attaaggcaa actactatca tgacaaaccc gatgccgatt tgtttgaagg tgcgatgaag
     2161 ggtatggtgg ccggtttgga tccgcattcc gaatatatgg ataaaaaagg ttatgccgag
     2221 ataaaggagt ccaccagcgg cgaatttggc ggcttgggga tggaaatcgg gcaggaagac
     2281 ggttttgtca aagtggtttc gccgattgag gacacgcctg ccgaacgggc ggaggtgaaa
     2341 agcggcgatt tcattgtgaa aatcgataat gtttcgacgc gcggtatgac ggtcagcgaa
     2401 gcggtgaaaa aaatgcgggg caagccgggt acgaagatta ctttgacgct gtcgcgcaaa
     2461 aatgccgaca agccgatagt cgtcaacctg acccgtgcca ttattaaagt gaaaagcgtc
     2521 cgccatcacc tgatcgaacc cgattacggc tatatccgcg tgtcgcagtt ccaagagcgg
     2581 acggtcgaaa gcgtcaatac cgccgcaaaa gagctggtaa aggaaaataa aggaaaaccg
     2641 ctcaaggggc tggtgttgga tttgcgcgac gaccccggcg ggcttttgac cggcgcggtc
     2701 ggcgtgtcgg cggcgtttct gccgtctgaa gcggtcgtcg tcagcaccaa gggacgcgac
     2761 ggcaaagacg gcatggtact gaaagccgtt cccgaggatt atgtgtacgg tatgggcggc
     2821 gatcctttgg cgggtattcc tgccgagttg aaaacgattc cgatggcggt attggtcaat
     2881 tccggttcgg cttccgcgtc ggagattgtc gccggcgcat tgcaggacca caaacgcgcg
     2941 gtcatcgtcg gtacgcagag cttcggtaaa ggttcggttc agactttgat tcctttgtcc
     3001 aacggcagcg cggtcaagtt gacgaccgcc ctgtattaca cgccgaacga ccgttccatt
     3061 caggcacagg ggattgttcc cgatgtcgaa gtaaaagata aggaacgtac ttttgaaagc
     3121 cgcgaggcgg acctggtcgg acacatcggc aatcccttgg gcggcgagga tgtgaacagt
     3181 gaaacccttg ccgtaccgct tgaaaaagat gcggataagc ccgctgcaaa agaaaaaggt
     3241 aaaaagaaaa aggacgagga tttgtcttca aggcggattc cgaaccctgc gaaagacgat
     3301 cagttgcgta aggctttgga tttggtcaag tcgcccgagc agtggcagaa gtctttgggg
     3361 ctggcggcga aaaagccggt ttcaaataaa gataaaaaag ataagaagta ggttttgacg
     3421 atgccgtctg aaagggtttc agacggcata ttatgtttga aaggacaggt tatgagcgat
     3481 tttttaccgg aatttgaacc gtgcggc
//
LOCUS       DAXDSD010000135         3443 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXDSD010000135 DAXDSD010000000
VERSION     DAXDSD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.135"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(385..600)
                     /locus_tag="ACFB2Q_001969"
     CDS             complement(385..600)
                     /locus_tag="ACFB2Q_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918930.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
     gene            complement(631..3399)
                     /gene="tdfH"
                     /locus_tag="ACFB2Q_001970"
     CDS             complement(631..3399)
                     /gene="tdfH"
                     /locus_tag="ACFB2Q_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC0918931.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRGVAQNYRVGGGGQHIGNFGEEYLERRKQQYFVQEGGLKFNAGSGKWER
                     DLQRQYWKTKWYKKYEDPQELQKYIEGHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSENEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          818 a          829 c          836 g          960 t
ORIGIN      
        1 acactgattg aatcaataaa attcaataaa attaaacagt tatgcaaata tatctttgta
       61 accgtgcatt tgcatacagt aaataaaaaa taacaacata aatcaattta ttacggtttt
      121 atttttattt ggtgttgacg ggcaacatat catctgcgcg gtaatgatga gattttaggt
      181 tgtgggattt atcggaaaaa cagaaacctc tccgccgcca ttctcacgaa agtgggaatc
      241 cggaaagcta aagccacagg aatttatcgg aaatggctga aatttatagt ggattaacaa
      301 aaatcaggac aaggcgacga agctgcagac agtacaaata gtacggcaag gcgaggcaac
      361 gccgtacctg tttttgttaa tccactataa aaaaactgat tcccgctttt gcgggaatga
      421 cgactcataa attacccgaa acaaccaaaa ataaccgaaa tcaaaaaccg gcttccctgc
      481 gtgggaatga aatggatttt tctttgagtt gccgatgcga taaaacggat tggcataggc
      541 ttgcggcagg atgatgcgtt tggggaaaca aatgccgtct gaagggcttt cagacggcat
      601 tgcagttttt ccacaaaacg cgggctgcct ttaaaacttg tagctcatcg tcatcaagaa
      661 ggtgcgtccg cgtgcgaaat tggtcaatac gcttttgctt gtgccgccgt atttgccgtt
      721 gcacaacgtt ttatcagcat tacacgttac gtcttcgtcc ttgtctttcg ggtcgaacga
      781 gctgtaataa cgctgcgttg ccgcatcatt gcccgcatcg agcggatcga tataacgcct
      841 gtcgaacagg tttttgactt cggcgcggaa aataaggttt ttcttcggct cgtaagcggc
      901 gtaaaaatca aaaatcaaag gctgtcgggc aagggtttcg gtttgtttga tggaacgctt
      961 gcccagttgc cggacattgc tggtatttcc cccgttggtg ccgtcgatat agcgttcttc
     1021 agccgtcgcg cggatgctct tgccgaaata gcgcatcgcg ccgcccaaag tcagtttgtt
     1081 gcccaaccag cgcgtaccga cttccaaacg tccgtaatct cgcggcaggg cggaaaccct
     1141 gctcagccca taaccttgtt tgagttggtc ttctttggag gcattgttgg gcgattcgct
     1201 cgcatcgctg aaattggtcg gttgcgtgct tttttgatag gcgtaagaaa ggttggtgaa
     1261 aaaacgccca taatcgtaat tcagctccag ctcaaaaccg tgtttgtgca ctttgtcttt
     1321 gaaattgcgg tgtcggatgg tgtaggcaag cccggtgctg ccgacccagc tcggaatatc
     1381 cccgttcaaa tcccaccatt tcccgtaaac gttgtggatg tagttgtcaa tgcggctgcg
     1441 gtagccgacc agtttcaatc ctaatatatc atcttgtttt aacaatcctt ttttataggt
     1501 attgaagcca aattgccaag tgtttgcgcg ctctggtttt aaggcggtgt gaacgccgga
     1561 gtcgccgatt tgggaaaaat acatttcttg gatgttgggc atacggtgtg tgcgcgaata
     1621 gccggcgaac ggcatgaaat aatcgccgaa gtccgcacta atgctgaccg aatggttgtt
     1681 ggcgcgcttt ttgccgtatt ttttcaatac gggttcataa agcccgcagc tcgggtcgca
     1741 atgttccttg tatgccggcg agttttctcc gaatgcccgc ttaaattcgt tttccgagcc
     1801 gtaatagccc gtatattcgc cgccgaaacg gtagttgatt gcattggtgc tgtagtttaa
     1861 gcggtaaatg tcttttttga gcgcggcatc gaagtagaac gtgttgaaat attgactgcc
     1921 ggccggttgg acgatggttg atttttgggg cagcagccct ttatcgccct taaaccgccc
     1981 caaataggaa taaagcccgt tgtcctgatc cggaccgtcg aaaaacagcc ccaattcttc
     2041 aggaaagcgg tttttgccgt attcgttgtg gaaataattg aagcccaaag tggtttgcaa
     2101 ctcggtttcg cggggcagcc ggaaggtggc ggtgttgttg aggtcgagga tttttgcgtt
     2161 gttgtaggtt tcaaaatcct ttaaaagccc ccagcctgta aacttcgacc ctttcggata
     2221 tttctgcctg cccgaattgt aggctgcggt cagattgagg ttggtatacg ggttcaaaga
     2281 caaaccgtaa ttgaattgat aattgcggtt gatgattttg cggctgccga ttttggtgtt
     2341 taaatcgcga aattgcgccg tgtatttatt gaatacgccg tcgtattcca atttaaacag
     2401 attacctgcc gactgctgct tcaggccgga cggatcgatg ggggtgatgt cgtattgcgg
     2461 cgccaggttt tcccgccagc ttttgtcatg accttcgatg tatttttgca gttcttgggg
     2521 gtcttcgtat tttttatacc actttgtttt ccagtattgc ctttgcaaat cccgttccca
     2581 ttttccgctg ccggcattga atttcaaacc accctcttgt acaaaatatt gctgtttgcg
     2641 ccgttccaga tattcttcac caaaatttcc gatgtgctgc ccgccgccgc ccacgcggta
     2701 attttgcgcc acgccgcgcc tgctgtgccc gtaaagcaca ccgacagatg ctccgctttc
     2761 cagccatttg cgcgcaccta tcgccgccat cgcattgcct ttggttgaat tggtgccggt
     2821 cagacctttt agcagcaggc cgtaggtatt attgccctga acgacgtcat ccacgcctaa
     2881 agtccgcaga ttcgccgaac cggcaaggct gttgatgcct gccgagccgc tgaagctgcc
     2941 tttgacgaca tccagtccgg caataaaatt gctgtcgaca gatgcaccga attgagatga
     3001 accgcctgcc ctgcccgcat cggtagaagt cgaataaaag gtctgcgtga tgccgtccac
     3061 catcgtattg acccgcccga acccgctgtc gccgcgaata ttcaaagaca caatgcccga
     3121 gcttttatct tgctgtgtaa acgcaccggg tatgctgcgt acgatgttgt cgaggttttc
     3181 gccggatttg aatatatcct gacgggtcga tacggcacgc gcatcggtaa acactttttt
     3241 gtctttcggt acgcgcttcg ccttgacgtg cacgtcttcc aaaacctgta tctgcgcctc
     3301 gctgcccgcg cgccctgcat cttctgcata actatgatga tatagcataa cacccataag
     3361 ataaaaacaa atcggcttca accggaaaga agatctcata ttttcctcaa caataaacaa
     3421 tcaatccaac atgtctaccg ttt
//
LOCUS       DAXDSD010000136         3302 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXDSD010000136 DAXDSD010000000
VERSION     DAXDSD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.136"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..1817)
                     /gene="carB"
                     /locus_tag="ACFB2Q_001971"
     CDS             complement(<1..1817)
                     /gene="carB"
                     /locus_tag="ACFB2Q_001971"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC0918932.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDT"
     gene            complement(1824..2372)
                     /locus_tag="ACFB2Q_001972"
     CDS             complement(1824..2372)
                     /locus_tag="ACFB2Q_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918933.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQEFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(2375..2938)
                     /locus_tag="ACFB2Q_001973"
     CDS             complement(2375..2938)
                     /locus_tag="ACFB2Q_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC0918934.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(3095..>3302)
                     /locus_tag="ACFB2Q_001974"
     CDS             complement(3095..>3302)
                     /locus_tag="ACFB2Q_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918935.1"
                     /translation="PSPPPRGREWVSADFSVAGGLKNNLNLQPLISGRLKKDARNINS
                     GNFSGSLYCQADGTNAASVFSDNL"
BASE COUNT          809 a          804 c          749 g          940 t
ORIGIN      
        1 gtgtcgaagt cggtggacac ggtttcgggg ttgcagttga ccataatcgt ttcaaagccc
       61 gattcgcgca gggcgagtgc ggcgtgaacg cagcagtagt caaactcgat gccctgaccg
      121 atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg acgggattcg
      181 cattcttctt cgtaagtgga gtaaagatag gcggtttcgg tggcgaactc ggcggcgcag
      241 gtatcgacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc gcgaacttct
      301 ttttcgctta cgttcaacaa ttgtgccaaa cgtttgtcgg agaagccttt gcgtttcaga
      361 cggcgtaggg cggcgtaatc caaatcttgc aactggccgt ctgaaaccga tttttcttcc
      421 ttcatcaagt cttcgatttg cgccaagaac caagggtcga tggcgcagat ctcgtggatt
      481 tcttccggcg tgaagcccgc gcggaacgcg tctgccacaa acagcatacg ttcggggccg
      541 gggttggcca gttcgcggcg gatttccgct ttgtcttcgc tgcgcggatt gaaaccgcac
      601 aagccggttt ccaaaccgcg caaggctttt tggaagcttt cctgaatggt acggcccatc
      661 gccattactt cgcccacaga tttcatctgc gtggtcaggc ggtcgtctgc ggcggggaat
      721 ttttcaaacg cgaaacgcgg gattttggtt accacatagt cgatggaagg ctcgaacgac
      781 gcgggcgtgc ggccgccggt gatgtcgttg cgcaactcgt ccagcgtaaa gccgaccgcc
      841 agcttcgccg ccaccttcgc aatcgggaag cccgttgctt tggaagccag cgcggacgaa
      901 cggctcacgc gcgggttcat ctcgatcaca atcatctcgc cgttttcagg gttcaccgca
      961 aactgcacgt tcgagccgcc cgtgtccacg ccgatttcgc gcaataccgc caacgaagcg
     1021 ttgcgcatga tttggtattc cttgtccgtc agcgtttgcg ccggcgcaac cgtaatcgag
     1081 tcgcccgtat gaacgcccat cgggtcgaag ttttcaatcg aacagatgat gatgcagttg
     1141 tccgccttat cgcgcaccac ttccatctcg tactctttcc agccgagcac agactgctca
     1201 atcagcagct catgcgtagg cgacgcatcg aaaccgcgtt cgcaaatcgc caaaaactca
     1261 tccttattgt aggcaatgcc gccgcccgaa ccgcccatcg tgaaagacgg acgaatcagc
     1321 gtcggaaagc cgacctgttc ttgcgccgcc aaggcttcgt tcatggtgtg gcagacaaaa
     1381 gatttcgggc aagagaggcc gattttttcc atcgcttctt taaagcggcc gcggtcttcc
     1441 gccttgtcga tcgcgtcttc cgttgcgccg attaactcga cattgtattt cgccagcacg
     1501 ccgttacgcg ccaaatccag cgcacagttc agcgcggtct gaccgcccat cgtgggcaga
     1561 atcgcatcgg gccgctcctt ggcgataatc ttctccaccg tctgccacat aatcggctcg
     1621 atgtaggtaa catccgccat ttcggggtcg gtcataatcg tggcggggtt ggaattcacc
     1681 aaaatgactt tatagccttc ttcacgcaag gctttgcagg cctgtgcgcc cgaatagtca
     1741 aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat ggattttagg
     1801 tcggtacgtt tgggcatggg tggttactct tgaattaaag aaggttgttt tgttacatag
     1861 cttttaagaa aagtatttcc cttttctaaa attgaagcta aaacatcatc ttctatgtta
     1921 gataaaatat tctctaaatc tgcgcttgat ttaattttaa agaattcttt tgtttgagaa
     1981 tggtatacaa gagaaggttt atttagctgt aaaagttctg ctatattatt taatgaacca
     2041 atactcttac catcccataa aataaagcca taatcagcaa tttctgccat tttcttatct
     2101 tttgctgtat aaaataccct tccagttcct ttgctgtcta cttggacaaa ctgccaattt
     2161 cccacattat ttcggtaaat tttgccagaa aagtaaatat gcacattagc ataatcttgt
     2221 tcttgtagaa attcttggat agctttatca gccccattag catctccaat aacaatatca
     2281 aaattattgc taagaatatt gttaattctt tctcgtattt gtggatttag tcgtgaaatg
     2341 ctgcgtgaac cagaaaagaa aatagtgcgc atttttatcc tctagtcttt gtcattgcta
     2401 aaacaattac agatctagca ttttttgaaa acaatttttc agttgatatt tccaatgttg
     2461 ctccgctatc aaataaatca tcgattacta aaatattctt attggataga tctatattgt
     2521 caattgtgat ggaattattc agtatttcta gtttttctga tttttcttca atatttttga
     2581 gtggtgtgtg tgagctagat tttcttaaaa taggagaata tggaatattc aagcgatcac
     2641 ttagttcttt ggcaattaat tgaactggtt gattaatacg ctctgttgta aaaggggcgg
     2701 gaacaattaa atttatagat tctaaaccac taaatttttg taaaatatag tctaccagca
     2761 aagaaacatt ttgggtttga ttacgatatt ttaattgata aacccattct ccaatcacac
     2821 ttctcttgct atcaaagtga ggatgcccta attcatcata acctaacaaa atactttttt
     2881 gcatatgatg gtctaaagca aatccttttg tccagttacc atttagttct atcgccattt
     2941 cattgactcc ttgtcaaaat taagtataac gagcaatcct agcaagccgt agcccgcatg
     3001 tagggtgtgc ggtacgcacg cacgcgttct tcattttccc tgtaaccccg acccaccgca
     3061 tgcgcgcctt gcggcacaca ctctaccgat gagttcaaag attgtctgaa aaaacgctcg
     3121 cggcgtttgt tccatctgct tgacaataaa ggctacctga aaaattcccg ctgttgatat
     3181 ttcgtgcatc ctttttcaga cgacctgaaa tcaacggttg caaatttaag ttatttttca
     3241 aaccaccggc aaccgaaaaa tctgccgaaa cccactccct cccccgtggg ggagggctgg
     3301 gg
//
LOCUS       DAXDSD010000137         3176 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXDSD010000137 DAXDSD010000000
VERSION     DAXDSD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.137"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(1..1020)
                     /locus_tag="ACFB2Q_001975"
     CDS             complement(1..1020)
                     /locus_tag="ACFB2Q_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFC0918936.1"
                     /translation="MGQSLFYVHLDDMTNDLTVHKANNFVQASYSMTLDEMRILALTL
                     GVFNPKNPSKRGFDFTVADFCKSFPDVNPDIAYTQVRNAVLKISKRWVTLVDNEHELT
                     EVALIHKRSYFKKEGRFYIEFHDELIPYISELHDNYTKYKLINIGALGSTHAIRLYEL
                     CSQYRDTGWRQTSVEDIKSWLSISDKYPLFKDFKKRVLTPSINEINAKSDLLVDVEPI
                     KRGRTIVALKFTIKSKKSAVKIEQKRPAFPHKNKYGKFVKLDTQNPKMSNAEYGNYAR
                     DCLKILEDFYSDLADVTTEDLRHYWVFLTSNASFRSKLGKRSDFLNELQNRGYKIVNC
                     ELVKV"
     gene            1423..1953
                     /locus_tag="ACFB2Q_001976"
     CDS             1423..1953
                     /locus_tag="ACFB2Q_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFC0918937.1"
                     /translation="MSVYLVEAQPAKVIAPTRTAINRIFTDKASGSSTDREGLDLLRM
                     KVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTIL
                     SAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLS
                     IARSTVYKILEDERAS"
     gene            2136..2996
                     /locus_tag="ACFB2Q_001977"
     CDS             2136..2996
                     /locus_tag="ACFB2Q_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-135"
                     /protein_id="HFC0918938.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTTPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          937 a          673 c          658 g          908 t
ORIGIN      
        1 tcacacttta actaattcac agttcacaat cttataccct cgattttgca actcgtttaa
       61 aaaatctgac cgcttaccaa gttttgatct aaaactagca ttgctagtca aaaaaaccca
      121 ataatgccgt aaatcttctg ttgtaacatc ggcaagatca gaataaaaat cttcaaggat
      181 ttttaggcaa tctctcgcat agtttccata ttcagcatta ctcattttgg gattttgagt
      241 atccaatttc acaaacttcc cgtacttgtt tttatgcgga aatgcagggc gtttctgttc
      301 gatttttacc gcacttttct tactcttgat cgtgaatttt aatgctacga ttgttcgccc
      361 acgcttgata ggttcaacat caacaagcag atcggattta gcattaattt catttatgga
      421 tggagttaat actcgctttt taaaatcctt aaacagtggg tacttatcag agatacttaa
      481 ccaactttta atatcttcta cgcttgtttg tcgccaacct gtatcacgat attgagaaca
      541 caattcataa aggcgaatag cgtgcgtact acccaaagcc ccaatattga tcaatttata
      601 ttttgtgtag ttatcgtgta attcagaaat gtaaggaatt agctcatcgt ggaactcgat
      661 ataaaatcgc ccttcttttt taaaatagga acgcttatga attaaagcta cttctgttaa
      721 ttcgtgttcg ttatcaacca gtgtaaccca acgctttgag atttttaaaa cggcatttct
      781 aacttgtgtg taagctatat caggatttac atcggggaag cttttacaaa aatctgccac
      841 cgtgaaatca aatccacgct tagacggatt tttaggatta aaaaccccca aagttaaagc
      901 cagaatccgc atttcatcaa gtgtcattga atagctggct tgtacaaaat tgttagcttt
      961 atggactgtt aaatcatttg tcatatcatc aaggtggaca taaaataaag attgtcccat
     1021 tataaccata cagttaaatg gtggtcaata aaaaacaaag accactataa caataaattt
     1081 gtccacctat aacaataaat ttgtccacct ataacaataa atttgtccac ctataaatct
     1141 cgcaagcctt gtgtaacaag gggagccaga gcctacaaac aagaatacaa acaagaatac
     1201 aaaaaaatag agcctaaagg ctctttttgg ggctttcagc cctaattttt tctttttttc
     1261 aggatttaaa attacaaaac ccttacagag caagtaaact tgtttgcttg ttctgcaagg
     1321 gttcgcaata aaaacaaccg ctaaacattt ctgcccagcg gttgaaaatt tacctattca
     1381 ccattacaat gatcaagcag gaaatttttt tgattgccgt aaatgtccgt atatctagtt
     1441 gaggcacaac ccgccaaagt cattgcccca accagaacgg cgataaaccg tatatttacc
     1501 gataaggcat ccggcagttc aacagaccgg gaagggctgg atttgctgag gatgaaggtg
     1561 gaggaaggtg atgtcattct ggttaagaaa ctcgaccgtc ttggccgcga cactgccgat
     1621 atgatccaac tgataaagga atttgacgct cagggcgtgg cagtccggtt cattgatgac
     1681 gggatcagta ccgacggtga tatggggcaa atggtggtca ccatcctgtc ggctgtggca
     1741 caggctgaac gccggaggat cctagaacgc acgaatgagg gccgacagga agcaaagctg
     1801 aaaggaatca aatttggccg caggcgtacc gtggacagga acgtcgtgct gacgcttcat
     1861 cagaagggca ctggtgcaac ggaaattgct catcagctca gtattgcccg ctccacggtt
     1921 tataaaattc ttgaagacga aagggcctcg tgatacgctt atttttatag gttaatgtca
     1981 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2101 ggtaaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat tttcgtgtcg
     2161 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2221 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2281 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2341 cttttaaagt tctgctatgt ggtgcggtat tatcccgtgt tgacgccggg caagagcaac
     2401 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     2461 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     2521 ataacactgc tgccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     2581 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     2641 aagccatacc aaacgacgag cgtgacacca cgacgcctgc agcaatggca acaacgttgc
     2701 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     2761 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     2821 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     2881 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2941 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3001 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3061 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3121 cgttccactg agcgtcagac ccctatctat aaactcttgg cttggttcta atccct
//
LOCUS       DAXDSD010000138         3163 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXDSD010000138 DAXDSD010000000
VERSION     DAXDSD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.138"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            44..757
                     /gene="minC"
                     /locus_tag="ACFB2Q_001978"
     CDS             44..757
                     /gene="minC"
                     /locus_tag="ACFB2Q_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC0918939.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            786..1601
                     /gene="minD"
                     /locus_tag="ACFB2Q_001979"
     CDS             786..1601
                     /gene="minD"
                     /locus_tag="ACFB2Q_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC0918940.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            1605..1868
                     /gene="minE"
                     /locus_tag="ACFB2Q_001980"
     CDS             1605..1868
                     /gene="minE"
                     /locus_tag="ACFB2Q_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC0918941.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            1872..2792
                     /gene="oxyR"
                     /locus_tag="ACFB2Q_001981"
     CDS             1872..2792
                     /gene="oxyR"
                     /locus_tag="ACFB2Q_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC0918942.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
BASE COUNT          791 a          659 c          855 g          858 t
ORIGIN      
        1 tagctaaggt ttgcgcggta aacttacatc attaaaaaat tctatgatgg tttatataat
       61 gaatgccttc gatataaagt cgacaaagat ggacgtattg tctatatctt tgcatacatc
      121 agacttgttt gatttggaag atgtgctggt caaattgggc aagaagtttc aagagtctgg
      181 tgttgttcca tttgttctgg atgttcaaga atttgattat cccgagtctt tggatcttgc
      241 tgcattggtt tcgttgtttt caaggcatgg tatgcaaatt ttgggtctga agcattctaa
      301 tgaacgttgg gctgctgtgg ctatgaagta tcatttgctg ttttgtctgt ctcattcgga
      361 aaatgttaaa gaactgggtc aggttgaggt gcagaaaacg gaggatggtc agaaagcaag
      421 gaaaacagta ttgattacat cccctgtccg taccggtcag caggtttatg ccgaagatgg
      481 cgatttgatt gttacggggg cggtcaacca gggggcggaa ttgattgcag atggcaatat
      541 gcatatttat gcgccgatga gagggcgtgc tttggccggc gccaaaggtg atacttctgc
      601 ccgcatattt atccactcca tgcaggcgga gttggtttct gtcgcgggga tttaccgtaa
      661 ttttgaacag gatttgccgg accatctgca caagcagccg gtacagatat tgttgcagga
      721 taaccgattg gttatcagtg caattggctc agagtaattg tttgatattt taaaaaggaa
      781 atattgtggc aaaaattatt gtagtaactt caggcaaggg cggtgtcggt aaaacgacta
      841 ccagtgcaag tattgcaaca ggtttggcat tacgcggata taaaactgcg gtaattgatt
      901 ttgatgtggg tttgcgcaac ctcgacctca ttatgggttg cgagcgtcgt gtcgtttatg
      961 acctgatcaa tgtcattcag ggtgaggcga cgctcaacca ggctttgatt aaagataaaa
     1021 attgtgaaaa cctgtttatt ttgccggctt cccagactcg ggataaagac gctttgacac
     1081 gcgagggcgt agaaaaagtg atgcaggagc tgtccggcaa gaaaatgggc tttgagtata
     1141 ttatttgcga ctctcctgct ggtattgagc agggtgcatt gatggcgttg tattttgctg
     1201 atgaagccat tgtaacgacc aatcctgagg tttccagtgt gcgtgactcc gacaggattt
     1261 tgggcatttt gcaaagcaaa tcccgtaagg cggagcaagg cggttcggtt aaagaacatc
     1321 tgttaattac gcgttattct cccgaacgtg tggcaaaagg cgaaatgctg tctgtacagg
     1381 atatttgcga tattctgcgt attcctttgc tgggtgtgat tcccgaatcc caaaacgtct
     1441 tgcaggcatc caattccgga gaaccggtca tccatcagga cagcgtagcg gcttccgagg
     1501 catataagga cgttattgcc cgtcttttgg gcgagaaccg tgaaatgcgt ttcttggaag
     1561 ctgagaaaaa aagcttcttc aaacgtctgt tcggaggata aggtatgtca ttgatcgaac
     1621 ttttattcgg tagaaagcag aaaacggcaa ccgttgcccg cgaccgcctt caaatcatca
     1681 ttgcccaaga gcgcgcccaa gaaggtcaga ctccggatta cctgccgact ttacgtaaag
     1741 agttgatgga agtcctgtcc aaatatgtga atgtttcatt agacaatatc cgtatttccc
     1801 aagaaaagca ggatggtatg gatgtgcttg agttgaacat tactttgccg gaacagaaaa
     1861 aggtatagga catgacctta accgaattgc ggtacatcgt cgcagtcgcc caagaacgtc
     1921 atttcggccg ggcggcgcgg cgttgttttg tcagccagcc cactttgtct attgccatta
     1981 agaaattgga agaagagctt gccgtctctt tgtttgaccg gagcagcaac gatattatta
     2041 cgaccgaggc gggggaacgt atcgttgcac aggcgcgtaa ggtattgaaa gaggcggagc
     2101 ttatcaggca tttggcaaat gaagaacaaa acgagctgga gggtgcgttc aaactcgggc
     2161 tgatttttac ggttgcgcca tacctgctgc cgaaactgat tgtctctttg cgccgtactg
     2221 caccgaaaat gcctttgatg ttggaagaga attacacgca tactttgacc gagtcgctca
     2281 aacgcgggga cgttgacgcg attatcgttg ccgaaccgtt tcaagagccg ggcattgtta
     2341 ccgaaccctt gtatgacgaa ccgtttttcg tgattgtccc gaaagggcat tcatttgagg
     2401 aactggatgc cgtttcgccc cggatgctgg gtgaggagca ggttttgctg ctgacggaag
     2461 gcaactgtat gcgggatcag gtactctcaa gctgttccga attggcggcg aaacagcgca
     2521 tacaggggct gaccaataca ttgcagggca gctcgatcaa tacaatccgc catatggtcg
     2581 ccagcggttt ggcaatcagc gtgttgccgg caaccgcgct gaccgagaac gatcatatgc
     2641 tgttcagcat tattccgttt gaaggtacgc cgccaagccg gcgggtcgta ttggcgtatc
     2701 gccgtaattt tgtccgtccg aaggcgttgt cggcgatgaa ggcggcgatt atgcagtcgc
     2761 agcttcacgg ggtaagtttt atccacgact aggcgcagaa aatgccgtct gaaacgggtt
     2821 tcagacggca tttgttatct ttgccgtcaa tcaggcaaac ctatccgacc tacagttgct
     2881 tcgaatcgtc attcccgcgc aggcgggaat ctaggtctgt cggtgcggaa acttatcgga
     2941 taaaacgctt gctccaatct cgcgttccag attcccactt tcgtgggaat gacgggatca
     3001 ggtttccgta tggatagatt cgtcattccc gcgcaggcgg gaatctaggt ctgtcggtgc
     3061 ggaaacttat cggataaaac ggtttctccg gtcccgagtc ctagattccc actttcgtgg
     3121 gaatgacggc atattttgac attgaataaa aaagactaaa aac
//
LOCUS       DAXDSD010000139         3099 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXDSD010000139 DAXDSD010000000
VERSION     DAXDSD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.139"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            112..2369
                     /locus_tag="ACFB2Q_001982"
                     /pseudo
     CDS             112..2369
                     /locus_tag="ACFB2Q_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            2382..3024
                     /gene="pgmB"
                     /locus_tag="ACFB2Q_001983"
                     /pseudo
     CDS             2382..3024
                     /gene="pgmB"
                     /locus_tag="ACFB2Q_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
BASE COUNT          779 a          932 c          786 g          602 t
ORIGIN      
        1 ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
       61 cactataaga tttcaccatt ccctcaaatc aatccaaaca ggagctttac gatgtacaca
      121 agaattatgg aaatcagccc ttggacgctg cgttcggcaa aactggaaaa agaacacaaa
      181 cggctgcaag agagcctgac cagcttgggc aacggctata tgggtatgcg cggcagcttt
      241 gaggaaacct actccgccga cagccacttg ggcacctaca tcgccggcgt gtggtttccc
      301 gacaaaaccc gcgtcggctg gtggaaaaac ggctatccca aatatttcgg caaagccatc
      361 aacgcgctca atttcagcaa agtcaaaatc tttgtcgacg ggcaagaggt ggacttggcg
      421 aaaaacgacg ttgccggctt ctccgtcgaa ctggatatgc aacacggcgt gttgcgccgc
      481 tcgttcaccg tattcggtgt gcgtttcgat gtgtgcaaat tcctgtcggt cgcgcaaaaa
      541 gagctggctc tcacccgctg ggaagccgtg tccgttgacg gcaaaaccca ccaagtccgc
      601 atcgattcca tcatcgatgc cgacgtgaaa aacgaagact ccaactacga agaaaaattc
      661 tggcaggtat tggacaaagg cgtttcagac gaccgctcct acattgccgc ccaaaccgtc
      721 gccaaccctt tcggtgtgga acaatttatc gtcaacgccg ggcaaacctt cgccggcagc
      781 ttcaaagcct tcggcggcag ccaaaccgat tggcaggtct ctaattcttt tgaagccgaa
      841 gtcggcggca cgcccgaaac ctttgaaaaa cgcgtgattg ttaccaccag ccgcgattat
      901 cagggcttgg aagcagtgaa agccgccggc cgcgccttat ctgaaaaaat cgcaggtgtt
      961 gcgtttgaaa ccttgctgga tgcgcacaaa gcaggctggc agcatcgttg ggaaatcgcc
     1021 gacgtggtca tcgaaggcag cgacgaagca cagcagggca tccgcttcaa cctgttccaa
     1081 ctgttctcca cctactacgg cgaagacgcg cgtctgaaca tcggccccaa aggctttacc
     1141 ggcgaaaaat acggcggcgc gacctattgg gacaccgaag cctacgccgt gccgctctac
     1201 cttgcactgg cggaacccga agttacccgc aacctgctgc aataccgccg caaccaaatt
     1261 gccgcaggtg cagcataacg cacgcgaaca gggcttggcg ggcgcactct atccgatggt
     1321 aacgtttacg ggcatcgagt gccacaacga atgggaaatc accttcgagg aaatccaccg
     1381 taacggcgcg attccttacg ccatctacaa ctacaccaac tacaccggtg acgagtgcta
     1441 tcttgccaaa gaaggcttgg aagtcttggt cgaagtgtcc cgcttccggg cggaccgcgt
     1501 ccacttctcc aaacgcaacg gcaaatacat gattcaaggc gtaaccggtc cgaacgaata
     1561 cgaaaacaac atcaacaaca actggtaaac caacaccctc gccgcatggg tattgggcta
     1621 cacccgcgaa gccttggcga aatacccgcg tccgggttcg aacgtgagcg ccgccgagtt
     1681 ggaaaaatgg gcggacatca gcgcgaatat gtaccgtccg catgacgaag aactcggcgt
     1741 attcgtgcag cacgacggct tccttgacaa agacatccgc cccgtatccg cgctttcgcc
     1801 cgacgatttg ccgctcaacc agaaatggtc gtgggacaaa atcctgcgtt cgcccttcat
     1861 caaacaggcg gacgtattgc aaggcatcta cttcttcggc gaccgtttcg acatggacga
     1921 aaaacgccgc aacttcgact tctacgaacc gatgaccgtg catgaaagct cgttgtctcc
     1981 atgtatccac tccatcctcg ctgccgaact gggcaaagaa gaaaaagccg tggaaatgta
     2041 ccggcgcacc gcccgcctgg acttggacga ctacaacaac gacaccgaag acggcctgca
     2101 tattacttct atgaccggct cgtggctcgc catcgtccaa ggtttcgccc aaatgaaaac
     2161 ttggggcggc aaactcagct tcgcaccgtt cctgccgagt gcgtggacag gctatgcctt
     2221 ccacatcaac taccgcggcc gtctgattaa agtcgccgtc ggcaaaaaac gtcgtcttca
     2281 ccctgctcaa aggcgatccg ctcgaattgc aggtgtacgg caaagacatc acgctcaacg
     2341 gcagccacac cgttgcattg gaaaaataag gatggcgcaa aatgactttt accgcagtgc
     2401 tcttcgatct cgacggcgtg attaccgata ccgccgaata ccactacctc gcatggaaaa
     2461 agcttgccga agaattgggc atcggcatcg accgcaagtt taacgagcag ctcaaaggcg
     2521 tgtcgcgcga cgattcgctc aaacgcatcc tcgcgcacgg cggcaaaacc gtcggcgaaa
     2581 ccgagttcgc cgaactgacc tgccgcaaaa acgacaacta cttggagatg attcaggcag
     2641 tcaaacccga agacgtgtac cccggcattt tgccgctgct ggaagcattg agggcaaacg
     2701 gcaaaaaaat cgccctcgcg tccgccagta aaaacggccc gttcttgctc gaacgcatgg
     2761 ggctgaccca cttcttcgac accgtcgccg accctgccgc cgtcgcgcat tccaaaccct
     2821 cccccgacat cttccttgcc gccgccgagg gcgtgggtgc ggacatacgc cgctgccggc
     2881 gtcgccgcca tcaaagcctc tggcgctttg cccatcggcg tgggcaaaac cgaagacttg
     2941 ggcagcgaca tcgcgctggt gtccgacacc gccgagctga cctacgccta cttgcaaaat
     3001 gtgtgggaac agtcgggcag ataaaattct aaggaagtaa aaaggccgtc tgaacagtgt
     3061 ttcagacggc ctttctgctt ttagaacaga atgataacc
//
LOCUS       DAXDSD010000140         3049 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXDSD010000140 DAXDSD010000000
VERSION     DAXDSD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.140"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(109..300)
                     /locus_tag="ACFB2Q_001984"
     CDS             complement(109..300)
                     /locus_tag="ACFB2Q_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918943.1"
                     /translation="MLPACSVSSLLLGEYLEFEENGTKIAAEIGSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(320..868)
                     /locus_tag="ACFB2Q_001985"
     CDS             complement(320..868)
                     /locus_tag="ACFB2Q_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918944.1"
                     /translation="MEKIINSKVRETNFNQAWRVIRAMQINNISDEQVMIVKMSCYLK
                     KDEIFKNIERYGYQSSDIKKINPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKPGETLYLNFKSIKNQCKNYFFAVLVLMPLTLKAVR
                     VKWQIIILFLMPFLYSLFNQII"
     gene            complement(856..1194)
                     /locus_tag="ACFB2Q_001986"
     CDS             complement(856..1194)
                     /locus_tag="ACFB2Q_001986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918945.1"
                     /translation="MKTFEKTWSAQYRDMEISVRNFWNLKRTGAEVYINGRRVYHNEA
                     GMASASLRSLMGEYLEFEESGTKITVEIGSAWHFNETIRNINTHLRGTKLPGSNWRVQ
                     DKGHFHLWKR"
     gene            complement(1239..1616)
                     /locus_tag="ACFB2Q_001987"
     CDS             complement(1239..1616)
                     /locus_tag="ACFB2Q_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918946.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     KILTVEVRSGLHFDLRNIYRIEM"
     gene            complement(1674..2039)
                     /locus_tag="ACFB2Q_001988"
     CDS             complement(1674..2039)
                     /locus_tag="ACFB2Q_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918947.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYKAYSETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(2021..2560)
                     /locus_tag="ACFB2Q_001989"
     CDS             complement(2021..2560)
                     /locus_tag="ACFB2Q_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918948.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGDV
                     GAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGI
                     GRHRPWESKSTDRSWKNRF"
     gene            complement(2603..>3049)
                     /locus_tag="ACFB2Q_001990"
     CDS             complement(2603..>3049)
                     /locus_tag="ACFB2Q_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC0918949.1"
                     /translation="PALIAFLESTVYEKSRNKASSGKSWLIGATTYKAIDPIVLSLTA
                     AYRINGSKTLSDDVKYKAGNYWMLNPNISFAANDRISLTGGIQWLGKQPDRIDGKKES
                     ARNTSTYAHFGAGFGFTKTAALNASARFNVSGQSSSELKLGVQHTF"
BASE COUNT          916 a          672 c          522 g          939 t
ORIGIN      
        1 taaagaaacg gcaacacacg ccaacagaaa aacatatttg aacttcatca tattttccac
       61 ataaaaggca gcctgaaaat ctttcaggct gcccttgtca aattattcct agctttcggc
      121 ttttttggca aaccaaacaa tccgattacc cgcataatac tttccattta ttgaaatccg
      181 acaagccgcg cccaaaaaat gccatgcact gccgatttcc gcagcaatct ttgtaccgtt
      241 ttcttcaaat tccaaatatt cacccaataa taaacttgaa acagaacacg cgggcagcaa
      301 gtgcccaccc tacgcttatt caaataattt gattaaataa agaataaaga aaaggcatca
      361 ggaacaaaat tataatctgc cacttgactc gcaccgcttt taaagtaagg ggcatcaaaa
      421 ccaaaaccgc aaaaaaataa tttttgcatt gatttttaat agatttaaaa ttcaaatata
      481 gtgtttctcc aggtttaaaa agtttatctt tatctatata tttgatgctt tccctatcca
      541 aaataaatat ttcaaacatt aaaaaatcat tacatgacca agccaaatca taaatttgat
      601 tcaaatgggt atcataaaga taaaaataat aaggtttggg aacaggtaaa atatttaatg
      661 gaaaaggagg attaattttc ttaatatctg atgactgata tccatatctt tctatattct
      721 taaaaatttc atctttctta agataacaag acattttgac aatcataacc tgttcatcag
      781 atatattatt tatttgcatg gcgcgtataa cacgccatgc ctgattaaaa ttagtctccc
      841 ttaccttaga atttattatc ttttccaaag atgaaaatgc cccttatctt gaactctcca
      901 gttagaacct ggaagtttcg tacctcttaa atgtgtatta atatttctta tagtttcatt
      961 aaaatgccac gcgctgccga tttcaacggt aattttcgta ccgctttctt caaattccag
     1021 gtattccccc attagcgaac gcaaagaagc agacgccatc ccggcttcgt tatgataaac
     1081 ccgcctgccg ttgatataga cttccgcccc tgtccgtttc aaattccaaa aattccgtac
     1141 tgaaatttcc atatcccgat attgtgcaga ccatgttttt tcgaaggttt tcataaaatt
     1201 tcctatacct gtccaatcgg cacatatcaa ttgcattatt acatctcaat acgataaata
     1261 tttcttaagt caaaatgcaa gcctgaccgt acctcaactg tcaaaatttt attatttttt
     1321 attgatttta agacaatttc tgaaaaattc tcttcgcttt ctcccttttg tagaagcaca
     1381 tcagaaaaaa taaaactttc ccgattaaat tcataaatat gtttcaacca ttcacctcct
     1441 ctttctgtaa ggcaagattc agtttcattc ttccttattg tataaatatt tccttcacaa
     1501 aatctgaaat aaatccataa atccatctta tccataatta aagaaaaagt ttcacctcga
     1561 gattttgtca acaattcgca aggttgcgat gttgcaatca aatagccgaa agacattttt
     1621 tacctcatac atggtcgaaa tcagtttctg ttagttcaga atccattttt tcgtcaacaa
     1681 ctgaatccgc atttttgaat taacgttttc atcagctgcc gtttatctaa accggcaggt
     1741 tcagtttcag aataagcctt atatgaagac tgtaagcatt tcagaaaaag atcatcagaa
     1801 ggcatatctg ccgaatcaaa tacaactgtt ttgattttgg tacttaccca aaaccctttt
     1861 tgctcttttt ctactatacg gaaattcaga atatttccaa ccgaatcaaa agcacggtaa
     1921 acatcatcca tcaaatcctg cggctctatt ttcttttcca attccgacaa tccttgaaat
     1981 atatccaaag acacatcttc aaatagaaaa aaaggaggag ttagaagcgg tttttccatg
     2041 atctgtccgt agattttgat tcccaagggc gatgacgacc aattccctgt ccaggcaaag
     2101 tcttgcccgt attatccgta actcgacgat gataatgggg aaattttcca ataggatgac
     2161 ctgttctatt accgaaaggg gctatccgca tattattgcc gattttaatc tcacgtccat
     2221 atttagcaaa ggaaacaacc tttcctgcag cacctacatc accaggaatt gcgcctaatc
     2281 cgccagcaac atctctaaca gaagctggtc tgcctgtcgt tgcataacta aaaccatgct
     2341 gtgtccacat accaatggca gcaccaccca agatagccaa tggaagaaaa gccccttcag
     2401 tctccttcat ctccttctga gaaagctccg ccaactgcat cggcgcatct gcccgcgtgt
     2461 ggaacacttg gtcttcaaat gcctgattgt ccaatccgtt tgccattgcg ggggcgatca
     2521 tagacagcat cattacggct gcggtgattt gttttttcat aataactcct ttggattaca
     2581 aggttggaaa atcaaaatct gcttaaaatg tatgctgtac gcccaatttc agttcggaac
     2641 tgctttgccc tgaaacgttg aaacgtgcgg atgcgtttaa agccgcggtt ttggtgaaac
     2701 cgaaacctgc gccgaaatgg gcgtaggtgg atgtgtttct tgcggattct tttttgccgt
     2761 ctatgcggtc gggctgtttg cccagccatt ggatgcctcc ggtcaggctg attctgtcgt
     2821 tggcggcaaa tgagatgttg ggattcagca tccagtaatt gcctgctttg tatttgacgt
     2881 cgtctgaaag ggttttgctg ccgttgatgc ggtaggcggc ggtgagggaa aggacaatcg
     2941 gatctatggc tttgtaggtg gtggcgccga tgagccacga ttttcccgac gaggctttgt
     3001 tgcgcgattt ttcgtaaacc gtgctttcga ggaaagcgat gagtgcggg
//
LOCUS       DAXDSD010000141         2956 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXDSD010000141 DAXDSD010000000
VERSION     DAXDSD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.141"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            156..359
                     /locus_tag="ACFB2Q_001991"
     CDS             156..359
                     /locus_tag="ACFB2Q_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918950.1"
                     /translation="MGGQYSSLENFALSVLREKIINNTVSSDEKLLIINDFLSGFLEF
                     DPETGEPVGETLKIEQMIDFFLV"
     gene            1236..1507
                     /locus_tag="ACFB2Q_001992"
                     /pseudo
     CDS             1236..1507
                     /locus_tag="ACFB2Q_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1743..2029
                     /locus_tag="ACFB2Q_001993"
                     /pseudo
     CDS             1743..2029
                     /locus_tag="ACFB2Q_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2454..2678
                     /locus_tag="ACFB2Q_001994"
     CDS             2454..2678
                     /locus_tag="ACFB2Q_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918951.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQGSIGKTEEPSYFLMFMFL
                     NSLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            2734..>2956
                     /locus_tag="ACFB2Q_001995"
     CDS             2734..>2956
                     /locus_tag="ACFB2Q_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC0918952.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQ"
BASE COUNT          941 a          477 c          600 g          938 t
ORIGIN      
        1 cccgccctaa tcagctttct tgaaagcacg gtttacgaaa aatcgcgcaa caaagcctcg
       61 ttaatcaaaa aaagggggct ttgccccttt tataacttaa ggataaatta tgaatattaa
      121 aaatgaaata gttgctttaa tttctaaaaa aattgatggg ggggcaatat agttctttag
      181 aaaattttgc tttatccgta ctaagggaaa aaattataaa caatactgtt tccagtgatg
      241 aaaaattatt gattattaat gattttttat caggttttct tgagtttgat cctgagacag
      301 gggaacctgt cggggagacg cttaaaattg agcaaatgat tgatttcttt ttagtctaaa
      361 tcatacatac cagtggacaa gctaggcgta gatccggctt ttaaatacga ttgtcaggtt
      421 tatacttggg ataaaatcaa aaataaagtt gtttaaacta tagtggatta acaaaaatca
      481 gggcaaggcg acgaagccgc agacagtaca gatagtacgg aaccgattca ctcggtgctt
      541 cagcacctta gggaatcgtt ccctttgagc cggggcgggg caacgccgta ctggtttttg
      601 ttaattcact atattgcatg gtgcatgcat taaatcagtc ttctagcgcc gcaatgggaa
      661 aacatagaag ataaacgaaa tggatgatct aatactatat tttttatcag gtatattcgg
      721 aaatcaagtt gctgaatata ttataaaaaa caaccgcgag ataaaagttc cctttattgc
      781 gctttacgct atatttttta cgttgattta tactgtggct ttgttgtttc tcagtctgat
      841 ctattgggtc aatggtgcgg agattgcatg gaaggggata ggtattttca gtatgtcggt
      901 cagtttttgt atagtcttct gtctttattt ggttgacaag gcaggaagat gtaaggataa
      961 gaaacaatag ggatcagatg cggcagcaaa cgtagattgg gtctaggctc aacctgcgtt
     1021 tgctgcatct ttggaagaaa tagtggattc gaaaaaatag gaagaattct gattaatggt
     1081 atgcgaggtg tagcagtggg ggctgttgct ggtggcataa ctggttatgc aggttctacg
     1141 ggtaagaaca ctgacattcg ccgttaaata ataaataaac caacttccaa ccaaacctgc
     1201 tatcggtttg gttggaagtg agaggaggta aggtagtgtt tgctgttatt ttcttttcga
     1261 cattgggctg tattttagca tggataagag atattcctaa aataaaatca aaaaaaattt
     1321 tggcaagatc cttatatatt ataggaataa taaatgtaat aatcagctat gtattaatta
     1381 aaaatatatt ggtttctgtt tcggatggag gtggaataaa atatgttgca atatatttat
     1441 caaatctttt ttttggacag tattgatgta tgctcttgtg aaaagattat ctaaaaagcc
     1501 aagctgaaac ccagtacaca acacatcaat aaagattcct tttgcacgga acaatgacaa
     1561 tattcaaatt gctggatttg caatctgatt tacctattga aattgggtgt gcaacatacg
     1621 ttctaagcag gctttgattt cccaaagagg ctgaagggta agagacgctt atatcataga
     1681 taaataatgg caaaacaggt aatgaattaa aggcattacc tgtttttaca gaaattttta
     1741 aaatagatga tttgattgca gctttttagg ctatttatca ggtaaataca gtgaagggaa
     1801 aataagcagg ttagaaatat ccctaacggc aggaagcatt ttctttgcag tattctttat
     1861 tcatgaactg ataattttta ttgttgatgg aaataaggaa aatctttgga attttattac
     1921 tgggataatg aaagatttat ttttattttc atgttctgtt tgtttattat tctatttctt
     1981 attattgatt gcagaaacca ttggaaaaac aatcaataag attaaataag atatttaagc
     2041 cgctaaaaaa ccatttgagt attttatctt ctatgcggca tccatcttgg aattgaaata
     2101 ctgcaatcgg gaggctaagg cttccgttgt aacgtttacc atatatttca ttattttaat
     2161 ttcatcaaaa aacaaaattc aagattatac ataccgccaa tcgtttcttg attgttttgt
     2221 atagtggagt aaatttaaat caggacaagg cgacgaagcc gcagacagta caaatagtac
     2281 ggaaccgatt cactcggtgc ttcagcacct tagagaatcg ttctctttga gctaaggcga
     2341 ggtaatgccg taccggttta aatttaatcc actatatatt tatccgatat atcaacatct
     2401 ctctatctta ttccgaattt tattgttgaa acttgcatta aaaaccatac aagatgatga
     2461 agaacaaata tattttaata aaagcagcaa tcggcataac cgccatatcc atattttatc
     2521 ttctgactca aggaagcatc ggcaaaactg aagaaccatc atatttcttg atgttcatgt
     2581 ttctcaactc gctttggttt gaagaaaata aaacagttat ggctgccgtt acggcaatga
     2641 ttgccgccca ctttatattt gtcgcactat ccgattaata agcaaacaaa aaggctgctt
     2701 ggaaatttca ggcagccttt caggaggatt ttaatggaac aaaaaaggcg gtttgccgcc
     2761 tccctgctcc tcgccgccgc cctgccgctt tgtgcacatt cttttccctt tgcggaagaa
     2821 aaccccatag cctacggcaa agtcaaaata cagagttgga aagcgcggcg ggatttcaat
     2881 attgtaaagc aggatttgga tttttcctgc ggggcggctt cggtggcgac acttttgaac
     2941 aatttttacg ggcaaa
//
LOCUS       DAXDSD010000142         2928 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXDSD010000142 DAXDSD010000000
VERSION     DAXDSD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.142"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..414
                     /locus_tag="ACFB2Q_001996"
     CDS             <1..414
                     /locus_tag="ACFB2Q_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC0918953.1"
                     /translation="WHTYFVKGDKAETEGVWVHNDCPTKLKPTERYNRQTHYGGSQTD
                     GARAQAARQAGEGKPCPTCGRIQIFGTKTAPSPQHEPPLVKHYYEHGGHSMSNADRAK
                     HARESIKGTQCLTCQRKEGAMMSRYSREQAKKHGL"
     gene            404..952
                     /locus_tag="ACFB2Q_001997"
     CDS             404..952
                     /locus_tag="ACFB2Q_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC0918954.1"
                     /translation="MDYNQTVLSHLQKFWKHHDIKGFTWTLGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDQFQLGKTVN
                     IGRPWVEQSSFRHFLISLPYPYGQELEYMDNVRFFWLLPITQTERLFLNTHSVEELET
                     KFDEAGIDYLDINRASTVWQAG"
     gene            1249..1602
                     /locus_tag="ACFB2Q_001998"
     CDS             1249..1602
                     /locus_tag="ACFB2Q_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918955.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            1592..1915
                     /locus_tag="ACFB2Q_001999"
     CDS             1592..1915
                     /locus_tag="ACFB2Q_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFC0918956.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIDGSQKNLQSIES
                     QIISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1974..2201
                     /locus_tag="ACFB2Q_002000"
     CDS             1974..2201
                     /locus_tag="ACFB2Q_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918957.1"
                     /translation="MEIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            2328..2871
                     /locus_tag="ACFB2Q_002001"
                     /pseudo
     CDS             2328..2871
                     /locus_tag="ACFB2Q_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
BASE COUNT          960 a          491 c          612 g          865 t
ORIGIN      
        1 tggcatacct actttgtcaa aggcgataag gcggaaacgg aaggggtttg ggttcataat
       61 gattgtccaa ccaaactaaa accgacagaa cgttataatc gtcaaacgca ttatggaggt
      121 tctcaaacag atggagcaag ggctcaggca gcaagacagg caggagaagg taaaccttgt
      181 ccaacttgtg gtagaattca gatatttggt acgaaaaccg caccaagtcc gcaacatgaa
      241 cccccattag taaaacatta ctatgaacat ggtggtcact ctatgagcaa tgctgatcga
      301 gcgaaacatg ccagggaaag cataaaagga actcaatgtt taacatgtca aaggaaagag
      361 ggtgcgatga tgtcaagata ttcgagagaa caggctaaaa agcatggact ataaccaaac
      421 tgttttatct cacttgcaaa aattttggaa acatcatgat attaaaggat ttacttggac
      481 tttaggaaga attgttgaag aacttcccga tttccaagtt ttccaagtga ttcctaatca
      541 tgaagatgag ccttgggttt atgtcagctc tgggataggt caatttttag ggcaggagtt
      601 ttttatcatc agcccgtttg aaacacctga acatattgag accttggcaa tgctggcatc
      661 tgcaagtatg cattatcctg atcaatttca attagggaaa accgtcaata tcggtagacc
      721 gtgggtcgag caatcatcct tccggcattt tctgatttct ctgccttatc cttatgggca
      781 agagttggaa tatatggata atgtccgatt tttctggtta ttgccaatta cccagacaga
      841 aagacttttc ttgaatactc attcggtaga agaattggaa acgaaatttg atgaagcggg
      901 tattgattat ttagatataa atcgtgcgag tactgtctgg caggcaggat aaaaattcgg
      961 caatcaaaat gccgtctgaa acaagtttgg actttcagac ggtattctga tgaatggaac
     1021 aggatactgg gaactgagtt cttcaaaaat cctatctccg tcccttatac gtcggtgctt
     1081 tagtcaaaac ggcagacgga tgaaaaatta ttgcccgtat ccttgccaaa gatgaagcaa
     1141 gatgaagcca ggtaattgaa tcggtacaag cgcaatgtcc gacttgttct gcaaaacagg
     1201 gagggagatt aagccaatat tcaaaagaac agaaaaagaa actgagtaat gggaataaaa
     1261 aatgaacaaa taataatttg taaaaaatat aatacagaaa tatatcctgt ttccgatgtt
     1321 tcaaaaatcg gtgttgctga aaatgtcaaa cagacaggcc tgtatccgat taacgggtta
     1381 aggcaccgtc ccaaaggtga tactaacggt tggtatatct gggccggtga aaacttctcc
     1441 tatgataaaa attttttcct gcccttgcat acgttccatt tgcaaatatg gcgacccgaa
     1501 atcatcccct ttttgacact tccgcccggt taccgttttt taattgggga aaacggttat
     1561 gaagacgtat ggtttgatga gttgctatta aatgataatt gacgaaaaat ctacttcaga
     1621 tgtactgaaa caatatcaag aactagggtt tgatttgaca aaacctatga taatagagtt
     1681 ttttatagat ggttctcaaa agaatctgca atcaatagaa agtcaaatca tttcttatcg
     1741 gcaagatttt caaatcagta ttgaacaaga tgaattcgga gaaatgtgga cttgctattg
     1801 ttccgtcaat atcgtccctt cccttgaaaa cattttagct attgaaacta cgttatttgg
     1861 tattgcacaa aaaaatgatt gtaattatga aggttttggt tcgtatggga actaacaaaa
     1921 agtgtaggtt gggttgaaaa aaccatattc ccattaaaga ttaggggatg tctatggaaa
     1981 taaaattcaa tactttgggg gttatcctga atggagtcaa tcctgaagaa aaattcataa
     2041 aaattattga tgaccaggaa aataccggtg gatttctgat actgttatct tcaaatgaca
     2101 aattctcatc atttgacagt tacgatgact gggtcgaaaa cttagaaata ttaaaagaat
     2161 acctgcaaga atctcattgg ataataaagt gggtcggata gtcggcaaac gtagtccgtt
     2221 tgaaacgctt ttacttttca gacggacttt ctaccaatgg gaacaaggcg ttttgtctca
     2281 atccggttta tgattattcg atacagtgat gggaagctaa gaaaaatatg attaatcata
     2341 ttgaaaaata tataggcact atttctcatg gctcgaaaag cgattcggga agtcagtaca
     2401 aattaaatat cgcagctatt ccctcttccc ctaacaggga tttaaaaact tatattactt
     2461 tagggttaag caaacatgat ctgaattata aaagcagatt tgaaatactt tttgtatgtt
     2521 ctttaaaata tgatgaaaat cagattttcc catttttaag atggttagca gaaaccatca
     2581 ttgagaataa aaaaaatcct tcttcggggg caggttgttt atttgcctag aagcattgtt
     2641 aattcaacaa aaatggacgc actgtatgtt tccgccccat tttatttcga cgatgatttc
     2701 caagtctgtt atggcgaaca ctacaatatt gttttccctt tgcttgtccc cttgtataaa
     2761 caggaagccg aattggtgga aaaaaagggt tggaatgctt ttgagcagtt cttgttggat
     2821 aatgaagttg gcaacctttc ggatatgaat aggaaaccgt ttgtttggta agggctttct
     2881 taaatttgat taaaggtcgt ctgaaattaa gcttgttttt cagacggt
//
LOCUS       DAXDSD010000143         2869 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXDSD010000143 DAXDSD010000000
VERSION     DAXDSD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.143"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..173
                     /locus_tag="ACFB2Q_002002"
     CDS             <1..173
                     /locus_tag="ACFB2Q_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HFC0918958.1"
                     /translation="EPLPPSALSESEANRDLGWMLHDIDFDHGNTPHFFRTQMKDGLI
                     DVPPFYAEEVKA"
     gene            170..1456
                     /gene="cas8c"
                     /locus_tag="ACFB2Q_002003"
                     /pseudo
     CDS             170..1456
                     /gene="cas8c"
                     /locus_tag="ACFB2Q_002003"
                     /inference="COORDINATES: protein motif:HMM:NF021243.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
     gene            1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFB2Q_002004"
                     /pseudo
     CDS             1476..2236
                     /gene="cas7c"
                     /locus_tag="ACFB2Q_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            2263..2631
                     /locus_tag="ACFB2Q_002005"
     CDS             2263..2631
                     /locus_tag="ACFB2Q_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918959.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHDPYDKDEWIEKIFCL"
     gene            2638..>2869
                     /locus_tag="ACFB2Q_002006"
     CDS             2638..>2869
                     /locus_tag="ACFB2Q_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HFC0918960.1"
                     /translation="MTALLTETQRENQDTRLIPLSALQHYAFCPRQCALIHNAQAWAE
                     NCLTAQGKALHERVDSDEPETHKGVRFERTVHV"
BASE COUNT          799 a          691 c          731 g          648 t
ORIGIN      
        1 gtgaaccgct gccgccgtcg gcgttatcgg aaagcgaggc aaaccgcgat ttgggctgga
       61 tgctgcacga tattgatttt gatcacggca acacgccgca ttttttccgc acgcaaatga
      121 aagacggctt gattgatgtg ccgccgtttt acgccgagga ggtgaaagca tgattcttgc
      181 gtcccttgtc cgctattacc gccgtttggc aacggaaacc gatgaaacgg gcaacccgaa
      241 agtgccgtct tatggtttta gcgaggagaa aatcggctgg attttggtgt tggataaaga
      301 aggtcgtctg aaaaccgttg tgccgaatct gactgccgat aaaaagccgc agccgaagct
      361 gatgagtgtg ccgcgccctg aaaaacgcac gtcgggtatc aaaccgaatt ttttgtggga
      421 taaaaccgcc tacgcgcttg gcgtggaagc caataaaaac aaagccgaag ccaaagaaaa
      481 accgtttacg ccgtctgaaa aaacctttga agccttcaag caataccatc tcgatttact
      541 gcaaaacagc gaagacgaag gtttacaagc cttatgccgt tttctgcaaa actggcagcc
      601 tgcacatttc gctgccgaaa acctgcctgc cgaaatgctt gattccaaca ccgcattttc
      661 tcttgaaaaa ccgaccgctc ttatccataa acgcgaagcc gcgcaaacct tgtgggcagg
      721 ctgcctgaaa agtgatgaag cactcgaaag cttatgcctg attagcggcg acaccgcgcc
      781 gattgcacgg ctgcatccgg cgattaaagg cgtgtttggc gggcaaagct ccggcggttc
      841 gattatttcg tttaataaag aagccttttc ctctttcgga aaagagcaag gcgcaaatgc
      901 gcctgtttcc gaacaatccg cctttgccta caccaccgcg ctgaactatc tcttgcgccg
      961 cgaaaataat cactgcctga ccataggcga tgccagcacg gtcttttggg cggaagcgga
     1021 tgatatagtg gattaaattt aaatcaggac aaggcggcga aaccgcagac agtacaaata
     1081 gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata acgccacggc
     1141 gcaggctgcc gaaggcttct tcgcgcaagt gtttacgccg tcggatgacg aacaagaaag
     1201 cgccaaaatt ttcaacgtat tggaacaaat cggcaaaggt cgtccgctgc aagaaattgc
     1261 gcctgaactc tctcccaata cccgttttta catcttgggg cttgccccca atgctgcgcg
     1321 gatttctgtt cggttttggc tggacaccac atttgggcag ctggcggaaa acttggcgca
     1381 tcattggcaa gatttagccc ttgagccttg tgcgtggaaa acgccgccgt ctatttggcg
     1441 gctcttgttg caaactgccg tgctgggcaa aagcgaaaac atccaaaggc ttggtaaccg
     1501 atgtctgcct gaaacgcaaa atccgtaatt ttgtcgaaat cagcagcgaa aacgaagccg
     1561 gttacgaaat ctacgtcaaa gaaaaaagcg tgctgaacct gcaaaacaaa cgcgcttatg
     1621 aagcactcgg cattgagtct gaagccaaaa actgccgaag gacgaagcca aagcccgtga
     1681 cattaccgcg tggatgtgta aaaacttctt cgatatccga accttcggcg ctgtgatgac
     1741 caccgaagtc aacagcggac aagtgcgcgg cccggtacaa ctggcgtttg cccaatccat
     1801 cgacccgatt gtgccgccgg aagtttccat cacacgcatg gcggtaacca acgaaaaaga
     1861 cttggaaaaa gaacgcacca tggggcgcaa atacatcgtc ccttacgtgg tctaccgcgt
     1921 gcatggcttt atctccgcca accttgccgc caaaaccggt ttttcagacg acgacttagc
     1981 caaactctgg caagccctga cgttgatgtt tgaacacgac cgctcagccg cccgtggcga
     2041 aatggcggca cgcaaactgg ttgttttcaa acacgacagc gcactcggca gccagcctgc
     2101 acataaactg tttgacaccg taaaagtcga acgcgtaaac ggcgaatcag gtacgcccgc
     2161 aagcggtttt ggcgattaca aaatcagcgt ggtttcagac gggctaaatg gcgtgagtgt
     2221 ggaagagtat ttgtaagttt tcaataaatc aggagtaacg atatgaaata ttggctagtt
     2281 ggagcctctt ggggagggca ggggcatcaa gatcaatttt ttgttgaaaa tggttactgg
     2341 gttttggagt gggaatcctc tcaacaacca gatcaatttg caaaaggaga aaaaatccaa
     2401 gtaggtgatc gaattgcaat caaacgcatg aaaggtcaag gatcgtctga aattaagatt
     2461 ttacatattg gaatcgttaa gggggttatt tctgaaacga acaaaattat ctgtgtcgtt
     2521 gattggatag ttaaaaattt agatagaaat gttgaaagta gaggatgctt taaaagtatt
     2581 catgatccat atgataaaga tgaatggata gaaaaaattt tttgtttgta gcaaaagatg
     2641 accgcacttt taaccgaaac ccaaagggaa aatcaggata cgcgcctgat tcccctttcc
     2701 gccctgcaac actacgcctt ctgcccgcgt caatgtgctt tgattcacaa cgcgcaggcg
     2761 tgggcggaga actgtttgac cgcgcagggc aaggcactgc atgagcgggt ggattcggac
     2821 gagcccgaaa cgcacaaggg cgtgcgtttt gagcggacgg tacacgttt
//
LOCUS       DAXDSD010000144         2686 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXDSD010000144 DAXDSD010000000
VERSION     DAXDSD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.144"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            281..1078
                     /gene="truA"
                     /locus_tag="ACFB2Q_002007"
     CDS             281..1078
                     /gene="truA"
                     /locus_tag="ACFB2Q_002007"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC0918961.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHAAAQVVHFDITAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PGFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAALLIGEQDFS
                     SFRAAGCQAKSPVKTIYRTDLTQSAGLIRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPGAYGIVRPQIPEWL"
     gene            1595..2641
                     /gene="porB"
                     /locus_tag="ACFB2Q_002008"
     CDS             1595..2641
                     /gene="porB"
                     /locus_tag="ACFB2Q_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB.IB"
                     /protein_id="HFC0918962.1"
                     /translation="MKKSLIALTLAALPVAATADVTLYGTIKAGVQTYRSVEHTKGKV
                     SKVETGSEIADFGSKIGFKGQEDLGNGLKAVWQLEQGASVAGTNTGWGNKQSFVGLKG
                     GFGTIRAGSLNSPLKNTKDNVNAWESGKFTGDVLEISRMAKREHRYLSVRYDSPEFAG
                     FSGSVQYAPKDNSGSNGESYHVGLNYRNGGFFAQYAGLFQRYGEGTKKIEYDNQFYSI
                     PSLFVEKLQVHRLVGGYDNNALYASVAAQQQDAKLYGTWRANSHNSQTEVAATAAYRF
                     GNLTPRVSYAHGFKGSVHSADYDNTYDQVVVGAEYDFSKRTSALVSAGWLQESKGADK
                     IVSTASAVVLRHKF"
BASE COUNT          695 a          816 c          635 g          540 t
ORIGIN      
        1 ccgccgtcat tcccgcgcag gcgggaatcc agacctgtcg gtgcggaaac ttatcggata
       61 aaacggtttc ttagattcca catcctagat tcccgcctgc gcgggaatga cggttcgggc
      121 actcctgaca gggtgaattc acaggatagc gattcgtagc aactgcatcc aaccccccga
      181 acaactcccc aaacaacgcc gcgcgccccg ggcgtttgcc gtttccctgc aaaatctgcg
      241 atacaatgca gtctgaacat ttatccgaat cccaaacccg atggataccg cacaaaaaca
      301 acgctgggca ataaccctat cctacgacgg cagccgcttt tacggctggc agaaacaggc
      361 gggcggcgta ccgaccgttc aggcggcatt ggaaaccgca ctcgcccgaa tcgcaggaga
      421 atctgtcgcc accacagttg ccggcaggac cgacaccggc gtgcacgctg ccgcccaagt
      481 cgtccacttt gacataactg ccgcccgtcc ccaacaggca tgggtgcgcg gcgtaaatgc
      541 ccacctgccc gaaggcattg ccgttttgca cgcccgacag gtcgcacccg gatttcacgc
      601 ccgcttcgac gcatacggac ggcactaccg ctacctgctc gaatccgccc ccgtccgttc
      661 ccccctgctc aaaaacaggg caggctggac acacctcaaa ctcgacatcg aaccgatgcg
      721 ccgggctgcc gccttattga tcggcgaaca agacttctcc agcttccgcg ccgccgggtg
      781 ccaggcaaaa tcccccgtca aaaccatcta ccgcaccgac cttacccaaa gcgcaggact
      841 gatccgcctc gatttgcacg gcaacgcctt tttgcaccat atggtacgca acatcatggg
      901 cgcgctcgtt tatgtcggca gcggcaggct cagcgtcgaa ggcttcgccg cactgattca
      961 agaacgcagc cgcctcaaag ccccaccgac cttcatgccc gacgggcttt acctgaccgg
     1021 cgtcgactat cccggggcat acggcatcgt ccgcccccaa atccccgaat ggctttaaaa
     1081 cattcttgtc gcggagattt tgaaatcgga caaactgtca ggcaatcttt ttcaatgttg
     1141 acactatagt ggattaacaa aaatcaggac aaggcggcgg gccgcagaca gtacaaatgg
     1201 tacggaaccg gttcgcccgg tgcttgggcg ccttagggaa ccgttccctt tgggccgggg
     1261 cggggcaacg ccgtaccggt ttttgttaat ccgctataac ccccggtttg caatgccctc
     1321 caataccctc ccgagtaagt gttgtaaaaa tgcaaatctt aaaaatttta aataaccata
     1381 tgttacaaaa caaaaaatac ccgtaatatc tctatccgtc cttcaaaatg cacatcggat
     1441 tccacacaaa aacaggcaga agtttgtttt ttcagacagg aacatctata gtttcagaca
     1501 tgtaaccgcc gagcccctcg gcggtaaatg caaagctaag cggcttggaa agcccggccc
     1561 gcttaaattt cttaaccaaa aaaggaatac agcaatgaaa aaatccctga ttgccctgac
     1621 tttggcagcc cttcctgttg cggcaacggc cgatgtcacc ctgtacggca ccatcaaagc
     1681 cggcgtacaa acttaccgtt ctgtagaaca tacaaaaggc aaggtaagta aagtggaaac
     1741 cggcagcgaa atcgccgact tcggttcaaa aatcggcttc aaaggccaag aagacctcgg
     1801 caacggcctg aaggccgttt ggcagttgga acaaggtgcc tccgtcgccg gcactaacac
     1861 cggctggggc aacaaacaat ccttcgtcgg cttgaagggc ggcttcggta ccatccgcgc
     1921 cggtagcctg aacagccccc tgaaaaacac caaggacaac gtcaatgctt gggaatccgg
     1981 caaatttacc ggcgatgtgc tggaaatcag cagaatggcc aaacgggaac accgctacct
     2041 gtccgtacgc tacgattctc ccgaatttgc cggcttcagc ggcagcgtac aatacgcacc
     2101 taaagataat tcaggctcaa acggcgaatc ttaccacgtt ggcttgaact accgaaacgg
     2161 cggcttcttc gcacaatacg ccggcttgtt ccaaagatac ggcgaaggca ctaaaaaaat
     2221 cgaatacgat aatcaatttt atagtatccc cagcctgttt gttgaaaaac tgcaagttca
     2281 ccgtttggtc ggcggttacg acaataatgc cctgtacgcc tccgtagccg cacaacaaca
     2341 agatgccaaa ttgtatggaa catggcgtgc taattcgcac aactctcaaa ccgaagttgc
     2401 tgctaccgcg gcataccgtt tcggcaactt aacgccccgc gtttcttacg cccacggctt
     2461 caaaggctct gttcatagtg cagactacga caatacttat gaccaagtgg ttgtcggtgc
     2521 ggaatacgac ttctccaaac gcacttctgc cttggtttct gccggctggt tgcaagaaag
     2581 caaaggcgca gacaaaatcg tatcgactgc cagcgccgtc gttctgcgcc acaaattcta
     2641 atctgcaaag atcggtatca acaaaaagcc tgtcgtcaga caggct
//
LOCUS       DAXDSD010000145         2676 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXDSD010000145 DAXDSD010000000
VERSION     DAXDSD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.145"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..360)
                     /locus_tag="ACFB2Q_002009"
     CDS             complement(<1..360)
                     /locus_tag="ACFB2Q_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFC0918963.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQ"
     gene            complement(447..2552)
                     /locus_tag="ACFB2Q_002010"
     CDS             complement(447..2552)
                     /locus_tag="ACFB2Q_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC0918964.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVPSKKPEARKDQGGYGFAMRFKRRNWYGSAKEDRVELSESDWEQTDNGNIKKPSKQ
                     QNIIDALSGNGEASLQDSSQQGEGISKVTDHHDFQYVWSGFFYKQIGSTIKKNGTSVI
                     EARNGPDGYIFYKGKDPSRELPVLGSVEYKGTWDFLTDVKANQKFTDLGNVSTQVGDR
                     YSAFSGELDYIVKKEDDKKEKHKGLGLTTEITVDFENKTLKGKLVKNNHVINDNSDDQ
                     NKYTTEYYTLDATLRGNRFSGKAMATDKSKNGETGDQTKKHPFVSDSSSLSGGFFGPQ
                     GKELGFRFLSDDGKVAVVGSAKTGDKAENGGGNGASGGASVSVSNGAAGTSSENGKLT
                     TVLDAVELTPDGKEIKNLDNFSNATRLVVDGIMIPLLPTESGDGQADKGENGKTAFIY
                     ETTYTPESDEKNTQAQTGAGGMQTVSNAAGGTSGETKTYKVQACCSNLNYLKYGLLTR
                     KTAGNTGGGGKGSQAAAQTAQGARSMFLQGERTDENKIPQGQDIVYRGSWYGYIVSGT
                     SWSGNASNATSGNRAEFTVNFDAKKINGKLTAENRSEATFTIDAMIDGNGFKGTAKTG
                     NGGFAPDQNSSTGTHKVHITSAAVQGGFYGPKAEELGGWFAYPGNGQTKNAQASSGNG
                     NSAGSATVVFGAKRQELVK"
BASE COUNT          566 a          695 c          586 g          829 t
ORIGIN      
        1 ctgtatttgc gccaagccgt ccaccgtcaa ggagacgcgg tttttgtcca taccgcgtat
       61 cgagtagccc gagcttgcgc cgcgcccctg ttcgacgacg gcgatgccgg ggtcgtaacg
      121 cgtcaggtcg cggatgtcga gtacctgttc tttgctgagg gtgtcggcgg ttttgaccaa
      181 tttgcccaaa ccggttactt cgttatcgcg gcgggttttc tgttttttgg cttttacctg
      241 tatggtatcc aactgttttt cctgtgcttg tccggcttgc acattttctg cataagcggg
      301 cagcgcagtc attaaagaca ggcataaaat attgaatcgg aacaaatgtt gctgttgcat
      361 agtgtttccc taatcttcgc tttcagacgg aatcggcagg aacgatgccg tctgaagcct
      421 tattctcgat tgttcggcag ccgtgcttat ttcacaagct cttggcgttt cgcaccgaat
      481 accacggtcg cgctgcctgc tgaatttcca ttgccggatg aagcttgcgc atttttcgtt
      541 tgtccattgc ccggataggc aaaccatccg cccaactctt cggctttagg cccgtaaaaa
      601 ccgccctgca ccgcggcact tgtgatgtgc actttatgtg taccggtgct gctgttttga
      661 tccggcgcaa agccgccatt accggttttc gccgtacctt taaagccgtt gccatcaatc
      721 atggcgtcaa tggtaaaggt tgcctccgac ctgttttcag cggttaactt gccgttaatt
      781 tttttcgcat cgaaattcac ggtaaattcc gccctgttgc cactcgttgc attggaagca
      841 ttgccgctcc agcttgtgcc gctgacaata tacccgtacc aagacccccg ataaacgatg
      901 tcttgccctt gtggaatctt gttttcatcg gtgcgctcgc cttgaaggaa catacttcgt
      961 gcgccctgtg ccgtttgggc ggcggcttgg ctgcctttgc cgcctccccc cgtgttgccg
     1021 gcggttttgc gcgtcagcaa cccgtatttc agataattga ggttggaaca gcaggcttgg
     1081 actttatagg tttttgtttc gccacttgtg ccgcccgccg catttgaaac ggtttgcatg
     1141 ccgcccgcgc ctgtttgggc ttgggtgttt ttttcatcgc tttccggcgt gtaggttgtt
     1201 tcgtagataa aggctgtttt gccgttttca cctttatctg cctgaccgtc cccgctttcg
     1261 gtgggcagga gcggaatcat aatgccgtcg acaaccagtc gggtagcgtt gctgaagttg
     1321 tcgagatttt tgatttcctt gccgtctggt gtcaattcaa ccgcatccaa aaccgtggtc
     1381 agcttaccgt tttcagacga cgtgcctgcc gcaccgtttg atacggaaac gcttgcaccg
     1441 cccgaagcac cgttaccgcc cccattttcg gctttgtctc cggttttcgc gctgccgaca
     1501 acggcaactt ttccatcgtc gctcaaaaag cggaaaccca attccttacc ctgcgggccg
     1561 aaaaagccgc cgctcagaga agacgagtcg gaaacaaagg gatgtttctt ggtttgatcc
     1621 ccggtttcac cattttttga tttgtcggtt gccatcgcct tgccgctgaa gcggttgccc
     1681 ctaagcgtcg catcaagggt gtagtattcg gtggtatatt tattttgatc atcactatta
     1741 tcattgatta catgattgtt tttaactaat tttcctttga gggttttgtt ctcaaaatca
     1801 accgttattt ctgttgttaa tcccaaacct ttgtgctttt cttttttatc atcctctttt
     1861 ttgactatat aatccaactc cccggaaaaa gcactatatc ggtcgccgac ttgcgtagaa
     1921 acattcccta aatctgtaaa tttctgattt gctttcacat cagttaaaaa atcccaagta
     1981 cctttgtact caacactccc taaaacagga agttctctcg aaggatcttt gcctttataa
     2041 aaaatataac cgtcaggacc gtttctggct tcgattacag atgtaccgtt ttttttaatt
     2101 gtactcccaa tctgtttata aaaaaaaccc gaccatacgt attggaaatc gtgatggtcc
     2161 gtaaccttag atataccctc accttgttga ctggaatctt gcaatgatgc ctccccattt
     2221 ccagataagg catcaataat attttgttgt ttggaaggtt ttttgatatt accattatcc
     2281 gtttgttccc aatcactctc gctcagttca accctgtctt cttttgccga cccataccaa
     2341 ttcctccgct tgaagcgcat tgcaaaaccg tatccgcctt ggtcttttcg ggcttccggt
     2401 tttttggaag gaacatcttg atactttggc gcgggacgcg gggcttcggt atcgacagaa
     2461 tcaagatcga aactgccgcc cccgcccaga caagcgctca acaaaaacac aggcagcacc
     2521 atagcagcct gattcaccaa tggattgttc ataacaaacc cgattcaatt aaagaatgat
     2581 aaggattatt attttattta tttttaaaaa atttgcaaat gcttttttat atttttagta
     2641 aataaaccaa aataccgtca ttcccgcgct gctttt
//
LOCUS       DAXDSD010000146         2653 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXDSD010000146 DAXDSD010000000
VERSION     DAXDSD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.146"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            322..540
                     /locus_tag="ACFB2Q_002011"
     CDS             322..540
                     /locus_tag="ACFB2Q_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918965.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
     gene            complement(557..916)
                     /locus_tag="ACFB2Q_002012"
     CDS             complement(557..916)
                     /locus_tag="ACFB2Q_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918966.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRHNLWHYHIGIPKYNGGRHGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(917..1456)
                     /locus_tag="ACFB2Q_002013"
     CDS             complement(917..1456)
                     /locus_tag="ACFB2Q_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFC0918967.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
     gene            complement(1616..2320)
                     /locus_tag="ACFB2Q_002014"
     CDS             complement(1616..2320)
                     /locus_tag="ACFB2Q_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC0918968.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEPTPVLINPDLPHEVKDIHRPMMWSSNDPLPDDDYVF
                     VPYLKESCFKGGAGAYEIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSMEEKIA
                     EDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYPDETAPL
                     DSLSVIGRVFWWSVLD"
     gene            2448..>2653
                     /locus_tag="ACFB2Q_002015"
     CDS             2448..>2653
                     /locus_tag="ACFB2Q_002015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918969.1"
                     /translation="MTHSEFIKTLSEQRGAKTEYAKKLGLSLSFLWQIENGRAVTPKR
                     LYKDVMLLTKNKVSISELISEFRV"
BASE COUNT          626 a          588 c          695 g          744 t
ORIGIN      
        1 ttaaggcggt tgtttaggaa agaatgccct ctgcgcgtgt agggtatttc gatacttgcg
       61 tttattatat catatgatat aataaactca tagattagga aatgaagaaa ccccgtatca
      121 gccgatacgg ggcaacctga aagagaaagg aggcaatgtg attaagaaaa tcctaatctg
      181 tgtgattttg tatttccttg caagctcccc agcgtggtag ggaaatacaa aacaagtgat
      241 taacgattaa ccatttgcag ggcagttaag gaggctgccc ttcctcttat ctttcccata
      301 ctctactata aggatttaaa aatggcaaat tccaacagcg ggcattctaa aaaattgcgc
      361 gcggcaacgg cggcggcggc gacaaaggca aaactggcaa gcggcgaata ccggcagttt
      421 tcagtgcagg ggcgtgccga agacgtggag cttatccttg ccgccgtgga aaaagccggg
      481 ggcagccgcg tacaggcttt ggcaaagatt tgcaggcggt atctcgaggg gctgtcttaa
      541 ggggggtaag acaaggttat ttgggaatat cgggcaggat aaaaggcggg tgtctgtcaa
      601 aaccgatgat tttgataaag ccgtcgcaaa gggtatagtg aaggatgtag gcggatgtca
      661 aatcgccatg cctgccgccg ttgtatttgg gaatgccgat gtgatagtgc caaaggttgt
      721 gccgttgggc aaatcggact ttttccagcc attgaggatc gtctgccggt acgttgtcgc
      781 tggatttgtt cctgccgggt aaccctgata agccgttctg ttggacgtgt gcgataaatt
      841 ccgcaatttt tctgcggtct tctttggggt agtttttcaa cgctcttttg aagtcttcgc
      901 caagcagcac ctgcatttac tgctccaacc atttttccaa gtcgtcggcg ccgttgaaat
      961 gcgggacgct gacaaagctg tcttcaacgg ctgttttgag tttttcaaga ttgaaattgt
     1021 aatccgtctg cggcagcgca tggcggaaat attcccccat cagcgcaatc cctatgactt
     1081 cgccctgccg ggtctgtatc cacggcgctt cctgatgggt tttattgcgt aatgcccaag
     1141 cggtgtagca gccctgctct ttacggacac ggttgaggac aaccaactct tcgtccgcat
     1201 atttgtccgg ttcgatatgg gccgcaggca atgggctgcc gccgtatttt ttgtaggtgc
     1261 ggtaaatgca ggggactacc ggaccgtgct gccagtgttc gatattttcg gcaaacaggg
     1321 ggcggttaag tatggcaagg gcgtagcctt gtgcgtaata caggagtttt tgaagtttga
     1381 gattggagat ttgctccccg tcctcttctt caaaagggga aaggaagaaa tctgccacgt
     1441 cgtacgcgtt cagcattttc tgtttccgtt gttatttttc tgtatattaa ggccgccggc
     1501 ccattatttc aagaaataat ttgtgcaaat tatccggtat cggcaggctg tccgcgccgt
     1561 cggcatatcg ggcggatata tttgaaaaat acttgccctg ccgtttcaga cggcatcaat
     1621 ccaacacgct ccaccaaaat accctgccga taacggacag gctgtccaaa ggggcggttt
     1681 cgtcgggata gaagccgctg ttgtggctgc gtatcagcac gctgttgccg ggctgccgta
     1741 tcaggtattt gacgcggaac atgccgtctt gggcgaaggc gtatattttg ccgtcgcgta
     1801 tggcggtttc gcccgtatct acggcgattg ccgcgtcttc tgcgattttt tcctccatgc
     1861 tgtcgccggt cagggtgcag cagaagacgt tgtcggggtt gatgcctttg cgccttaacg
     1921 ttgatttgcc gaacggcagg cggtagccgt tgtagtcggg gatttcatac gcgcccgccc
     1981 cgcctttgaa gcagctctct ttgaggtagg ggacgaaaac ataatcatcg tcgggcagcg
     2041 ggtcgttact gctccacatc atcgggcggt ggatgtcttt gacttcgtgc ggcaggtcgg
     2101 ggttgatgag gacgggcgtt ggttcgctcg gaacggaatc caagtacaga ctgcccatcc
     2161 catattcttc ttcaagccgt ctgcttacct tttcgccaaa agatgcttta ccgctcatca
     2221 attgagataa aaggcttttg tctttaaccg gcaacttttt attttcaaag aatcttttta
     2281 aattatcgat acggatggcc ttcagatctc tattagtcat tttcgcgctc cattcagatt
     2341 gggattcaat tttatttagt ttctgctaaa caaacaaata ttaaattttc ttgcctatgc
     2401 gtttaatatt tgctaaactc attaaatatt tacggtgaaa ctacagaatg actcattcag
     2461 aatttattaa aacgctgtca gaacagcgtg gtgcaaaaac ggaatacgct aaaaaacttg
     2521 gcttatccct gtcttttcta tggcagatag aaaacggacg ggcagtaacg ccaaagcgtc
     2581 tttataaaga cgttatgttg ttgacaaaaa ataaggtttc tatttctgaa ttaatttcgg
     2641 agttcagggt ttg
//
LOCUS       DAXDSD010000147         2627 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXDSD010000147 DAXDSD010000000
VERSION     DAXDSD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.147"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            68..205
                     /locus_tag="ACFB2Q_002016"
     CDS             68..205
                     /locus_tag="ACFB2Q_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918970.1"
                     /translation="MSREQRIRGARLPVSAYMQAEGAGKAREALDGRVEKVKGVDDEA
                     V"
     gene            750..1253
                     /locus_tag="ACFB2Q_002017"
     CDS             750..1253
                     /locus_tag="ACFB2Q_002017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918971.1"
                     /translation="MNADAFVSADAETCGRETGEPTSPKAESDSNGNGGLSGKPKNAN
                     VPRRRKTHGVPLQEIADLYNEVLGGRLPSVQVLNDTRKRAIANRWCEMLGTAAPNGKV
                     RFGDKETGLAWFAGFFRKVAMNPFWMGENQTGFAVGFDWIFKAGNFVKILEWHPPKTN
                     QAARGRA"
     gene            1250..2611
                     /locus_tag="ACFB2Q_002018"
     CDS             1250..2611
                     /locus_tag="ACFB2Q_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFC0918972.1"
                     /translation="MNRIEETEAVQSLASVGAEQNILGGILIEPTAIARCAILTPEKF
                     YQAQHRIIFRALLDMAAANEPIDIITLNDKLEARGEAENAGGLAYLIDLNQNTPSAKN
                     ISRYVGIVNDRFVERGLLKASAAIEKIAVSKDGGTVAEKLSKAADELAAAGKDAVKRE
                     TKTFGQTVEDLIGGLDKRLDGVRFGLPTGLMKLDGMTGGLPDGNLIVIAARPSMGKTV
                     LAENIARFALKQGKAVHFQSYEMSAVELARRGMAAECNIPMQNLKTGNLTQSDYANMP
                     IYVSQAKEWKFDVNCDLLNVDELCFLAKEKKLTTGLDLLVVDHLHIMPRAGRDEVAEL
                     GNISRRLKNLAAELNIPVVLVAQLNRGNTKQADKRPNMADIRGSGAIEQDANIIIMPH
                     RESYYDGNENPSIAELIIAKNRDGETGTVVCGWKGQFMKFEEEPDLAWQAPKHDEYDP
                     YSV"
BASE COUNT          722 a          614 c          798 g          493 t
ORIGIN      
        1 aatacaaggt cggtaactgt aaaaatcccg ttgcccatta ctcaatcgca aagtcaggaa
       61 ttgaaccatg agccgggaac aaagaatacg cggagcccgc ttgccggtgt cggcttatat
      121 gcaggcggaa ggcgcgggca aggcacggga agcgttggac gggcgggtcg aaaaagtgaa
      181 aggggttgat gatgaagccg tctgaaagtt tgagagcggc aggcaggcca atcgcgtatt
      241 acccgaaatt ggcaaagcct ttgggcggtg taaatgcggc aatattattc gggcatttct
      301 tctactggaa cgataagacg cagtatgaat caggcattta ccgaacagcg gaagaaattg
      361 aaattgaaac cgggctgtcc gttcaggaac aaagaacggc acgggcaaag ctgagggaac
      421 gcggcgtatt gattgagact gaaaaacgaa ttgaacaccg catttactac aaactgaatt
      481 tagacgcttt tgatgatttg atgttgcaac attcgggggg tggggaatca acagccccga
      541 aatgcaatat caacagcccc gaacttcaaa atcaacattc ggggggtggg gaatcaacag
      601 ccccgaaatg caatatcaac agccccgaac ttcaaaatca acattcgggg agtgaggaat
      661 caacagccgt tataagaaca gaagatttaa cagaagattt agcagtatat acccccttac
      721 ccccaaacgc cggaaacggc aaaggcggtt tgaacgctga cgcgtttgtt tccgctgacg
      781 cggaaacgtg cgggcgggaa accggcgaac cgacttcgcc gaaggccgaa agcgacagta
      841 acggcaacgg cggcctttcg ggaaaaccga aaaatgcgaa tgttccgcgc cgccgcaaaa
      901 cccacggcgt accgcttcag gaaatcgccg atttgtacaa cgaagttttg ggcggccggt
      961 tgccaagcgt ccaagtgctg aacgacacgc gcaaacgggc gattgcaaac cgctggtgcg
     1021 agatgctggg aacggcggcg ccaaacggca aggtgaggtt cggggacaag gaaaccggtt
     1081 tggcctggtt tgccggtttc ttccggaaag tggcgatgaa cccgttttgg atgggcgaaa
     1141 accaaacagg gtttgcggtc ggcttcgatt ggattttcaa ggcgggcaat ttcgtcaaaa
     1201 tccttgaatg gcatccgcct aaaacgaacc aggcggcaag gggaagggca tgaaccgaat
     1261 cgaggaaacg gaagccgtcc aatcactggc cagcgtaggg gcggaacaga acattttggg
     1321 cggcatcttg attgaaccga cggcgattgc gcggtgcgca atcctgaccc ctgaaaagtt
     1381 ttaccaggcg caacacagga ttattttccg cgctctgctg gatatggcgg cggcaaacga
     1441 gcctatcgac atcatcacgc tgaacgacaa gctggaagcg cgcggcgagg cggaaaacgc
     1501 gggtggcctg gcttacttga tagacctgaa ccaaaacacc ccaagcgcga agaatatcag
     1561 ccggtacgtt gggattgtga acgacaggtt tgtcgagcgc ggcttgctga aggcttcggc
     1621 ggcgattgaa aaaatcgcgg tttccaaaga cggcgggacg gtcgcagaaa agctgtctaa
     1681 ggcggccgac gaattggcgg cagccggcaa agacgcggta aagcgtgaaa ccaagacatt
     1741 cggccagacc gttgaggatt tgattggcgg tttggacaaa aggcttgacg gcgtgcgttt
     1801 cggattgcct accggcttga tgaagcttga cgggatgacc ggcggtttgc cggatggaaa
     1861 cctgattgtg attgcggcgc gtccgtctat gggtaaaacg gttttggcgg aaaacattgc
     1921 gcgattcgcg ctgaagcagg gcaaggcagt tcatttccaa agctacgaaa tgagcgcggt
     1981 agagctggca aggcgcggca tggcggctga gtgcaatatc cccatgcaga acctgaaaac
     2041 cggcaatctg acgcaaagcg attacgccaa tatgccgatt tacgtcagcc aagcgaaaga
     2101 gtggaagttt gacgtgaact gcgacctgct caacgttgac gagctttgct ttttggctaa
     2161 ggagaaaaaa ctcactaccg gcttggattt gttggttgtc gatcaccttc acatcatgcc
     2221 aagggcaggg agggacgagg tggcggagtt ggggaatata tcgcgccgtt tgaaaaactt
     2281 ggcggcagag ctgaatatcc ccgtcgtctt ggttgcccag ttgaacaggg gaaacacaaa
     2341 gcaggcagac aaacgcccga acatggcaga cattcgcggc agcggcgcga ttgagcaaga
     2401 cgcaaacatc atcatcatgc cgcaccgcga aagctactac gacggaaacg agaatccgag
     2461 cattgccgag ctgattatcg ccaagaaccg ggacggcgaa acgggaacgg tggtttgcgg
     2521 ctggaaaggg caatttatga agttcgagga agagcctgat ttggcatggc aagcccccaa
     2581 acatgatgaa tatgaccctt acagtgtcta gtgcgggagg ccggtaa
//
LOCUS       DAXDSD010000148         2608 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXDSD010000148 DAXDSD010000000
VERSION     DAXDSD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.148"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(158..571)
                     /locus_tag="ACFB2Q_002019"
     CDS             complement(158..571)
                     /locus_tag="ACFB2Q_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918973.1"
                     /translation="MIHLNVHYCDEIIRHAENDKYSLIGIFPDICHIPTPQAILGRLC
                     LSVSFSAEGMDIQTMKTGQIFLEIVRNDDVISALEIPSYDGSDTEENVSFMLHQTISG
                     LPVSDNDRIYVRMTTHNHILSESRPLSFSWLPYHS"
     gene            complement(568..1029)
                     /locus_tag="ACFB2Q_002020"
     CDS             complement(568..1029)
                     /locus_tag="ACFB2Q_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC0918974.1"
                     /translation="MGATLTVAVAACTVSTPTSAIPVTHIKCLRINGQIKCVKPISPN
                     TTPAAEHIEHVRKNPRRKAAMDRAAARIADKIALKAGGETFVSLRMKKGFTQSELATA
                     AGLPQPYLSRIENSKQSLQDKTVQKLANALGVSPLEVRAAFERRYEYMEQA"
     gene            complement(1046..1483)
                     /locus_tag="ACFB2Q_002021"
     CDS             complement(1046..1483)
                     /locus_tag="ACFB2Q_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918975.1"
                     /translation="MELTVHFNAEQDLDRLFEKDEEAVGYLENVIAMIQADSAIFDGL
                     YKNRYFREYGEPIGPIDLEVKPILSLWGKDIKVLRVRFDSEEAAGYRIIYAPCHEKQP
                     NGTYIRRIDILAVVNKKTDEFDYQAEHPITKRIIKDYEELYSN"
     gene            complement(1598..2314)
                     /locus_tag="ACFB2Q_002022"
     CDS             complement(1598..2314)
                     /locus_tag="ACFB2Q_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC0918976.1"
                     /translation="MPFIIDYQCLCAINSEIKMFSGEQLGQAISEAIKRKNVSQKEVA
                     DHFGVKQPSVSGWIKNGRIDKKHLDKLIDYFSDVVTPSHFGIETFRVLKSNEQSSIRF
                     PRLNAEATCGAGTINDHYIEVVDYVTVAAAWAREKLGGNLNKIQVITARGDSMEPTIE
                     NGDVMFVDTAVEAFDGDGLYLLWYIDGLKAKRLQSTVGGGLMIISDNSSYRTETVRGE
                     DLNAVRIIGRIRGAWRLSQF"
     gene            2383..>2608
                     /locus_tag="ACFB2Q_002023"
     CDS             2383..>2608
                     /locus_tag="ACFB2Q_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC0918977.1"
                     /translation="MAMSIQKAVDYFGNESRLARAIGVKQPTVWAWNKKGTPPPIIRC
                     VQIEKLTGGAVNRKDLRPDDWHLIWPELAGD"
BASE COUNT          634 a          562 c          625 g          787 t
ORIGIN      
        1 tataactatt acctatattt aatcaatagg caatagttat attttcatcc atatatactt
       61 attgaattga tttttagaag aaaaaagttt gaaaaaaaac cgcccgaatg gcggtgtatc
      121 atgaaaaaga aaaccgccct agggcggttc ggtattgtta tgagtggtaa ggaagccaag
      181 aaaaagacaa aggacggctc tcggagagaa tatggttgtg tgttgtcatt cttacataaa
      241 tgcggtcgtt gtctgaaacg ggtagtccgc tgattgtttg gtgtagcatg aatgacacgt
      301 tttcttctgt atcactgccg tcatatgatg gaatctcaag tgcagagatt acatcatcgt
      361 tgcggacgat ttccagaaaa atttgaccgg ttttcatagt ctgtatatcc atgccttcgg
      421 cggaaaatga aacagacagg cacaggcggc ctagaatggc ctgcggtgtc ggaatatggc
      481 atatatcggg gaatatacct atcaggctgt atttgtcatt ttccgcatgg cggataattt
      541 catcgcaata gtggacattt aagtgaatca tgcctgctcc atatattcgt accgccgttc
      601 gaacgctgcg cgaacttcaa gcggggaaac gcctaatgcg tttgccaatt tttgtacagt
      661 cttatcttgc agggattgtt tgctgttttc gatgcgggac agatagggtt gaggcaatcc
      721 tgcggcggtt gccaattcgg actgggtaaa cccctttttc atccttaagc tgacaaatgt
      781 ttccccgcct gctttgaggg ctattttatc ggcaattctt gctgccgccc tgtccatcgc
      841 tgctttgcgg cgcggatttt tccgcacatg ctcaatatgc tctgcggcag gggtagtgtt
      901 agggcttatc ggtttgacgc atttaatctg cccgtttatc cgcaggcatt ttatgtgggt
      961 gacggggatg gcggatgtag gtgttgatac tgtgcaggcg gccacggcaa cagtgagtgt
     1021 tgcacccatt ttgaaccatg tgccgttagt tggagtacag ttcttcatag tctttgataa
     1081 tccgttttgt gattgggtgt tctgcctgat agtcaaattc gtctgtcttc ttgtttacaa
     1141 cagccaatat atctatgcgg cggatatacg tgccgtttgg ctgtttttca tggcatggtg
     1201 cgtaaattat tctgtatccg gcggcttctt cgctgtcaaa tcggacgcgt aaaactttga
     1261 tgtcttttcc ccataatgac aatatgggtt tcacttccaa gtcaatcggg cctatgggtt
     1321 cgccatattc cctgaagtat ctgtttttgt ataagccgtc aaaaatagca gagtccgcct
     1381 gaatcatcgc aatgacattc tcgagataac cgaccgcttc ttcgtctttt tcaaagaggc
     1441 ggtctaaatc ctgctcggcg ttaaagtgga cggtcagctc catgagtgcc ctttttatat
     1501 atatatatta tatcttttta gttataccgc gcaatatgcg gtaataaatc agttgttcaa
     1561 gtattttaat ccggcacgct ccaccagaag acgcggtcta gaactggctc aaacgccatg
     1621 cgccgcgtat gcgtccgatg atgcgtacgg cgtttaaatc ttcgccgcgc acggtttcgg
     1681 ttcggtatga gctgttgtcg ctgatgatca tcaggccgcc gccgacggtg gattgcagcc
     1741 gcttggcctt aaggccgtct atataccaaa gcaggtagag gccgtcgcca tcgaaggctt
     1801 cgacggcggt atcaacgaac attacgtcgc cgttttcgat ggtgggctcc atgctgtcgc
     1861 cacgggctgt aatgacttgg attttgttga ggtttccgcc cagtttctcc cgcgcccatg
     1921 cggcagcgac ggttacataa tccacaacct cgatatagtg gtcgttaatc gtgcctgcgc
     1981 cgcaggtcgc ttcggcattt aagcggggga aacgtatgct actttgttca ttcgatttta
     2041 agactctgaa tgtttcaatg ccgaaatggc ttggcgttac tacgtctgag aaataatcaa
     2101 ttaatttatc tagatgtttt ttatctattc gtccattttt tatccaacct gaaacgcttg
     2161 gctgtttcac cccaaaatga tcggcaacct ccttttgact gacatttttt ctcttaatcg
     2221 cttctgatat tgcttgtcct aactgttcgc ctgaaaacat tttgatctcc gaattaattg
     2281 cgcataagca ttggtaatcg ataataaaag gcataaccta taaaaggcaa tagttgtatt
     2341 taatataagt attagctata atgaagctat ttaattgagt aactggctat gagtatccaa
     2401 aaagcagttg attattttgg taatgaatcc cgacttgcac gggcgatcgg agttaaacaa
     2461 ccgacggtgt gggcttggaa taaaaaagga acgccgcccc cgatcattcg gtgcgtgcag
     2521 attgaaaaat taaccggagg cgcagtgaat cgaaaagact tacgtcctga tgactggcat
     2581 ctaatctggc cggaattggc aggcgacc
//
LOCUS       DAXDSD010000149         2606 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXDSD010000149 DAXDSD010000000
VERSION     DAXDSD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.149"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            99..728
                     /locus_tag="ACFB2Q_002024"
     CDS             99..728
                     /locus_tag="ACFB2Q_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC0918978.1"
                     /translation="MIQNVVTSIILYSGTAVDLLTYPNVIFCQKKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGESIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILIENNSFDMLWTVSGQEKIL"
     gene            874..2550
                     /locus_tag="ACFB2Q_002025"
     CDS             874..2550
                     /locus_tag="ACFB2Q_002025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC0918979.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVIGLTSCGFAGFNFLGRHHGRSVVLIHIGCIGLIPVAHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLNYHVFGTFGGVRHIQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAFNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASFSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     RCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
BASE COUNT          507 a          670 c          674 g          755 t
ORIGIN      
        1 ggttttgtta atccactata ttttgacggg gttgaagttg acatctacaa agtttaatgt
       61 ttgaatacat taaaaaatat atttgaatgg gatgttttat gattcaaaat gttgttactt
      121 caataatcct gtattctggg acagccgtag acttacttac ttatcctaat gttatttttt
      181 gccaaaaaaa aagcagaaaa gacatcatta acatctattt aggacaattt ctaggctctg
      241 ttagcctgat attgctaagt ttgctttttg catttgtctt agattatatt cctagtaaag
      301 agattttagg tttgctcggc ttgattccga ttttcctagg cctcaaagtt ttgctgttag
      361 gagattccga tggagagtct attgccaaag agggtttgcg caaagataat aaaaacctga
      421 tttttctagt cgctatgatt acttttgcaa gttgtggcgc tgacaatatt ggtgtctttg
      481 tcccatattt tactacctta aatttagcga atttgatagt ggctttactt acctttctag
      541 ttatgattta tctcttggtt ttttctgccc aaaaattggc acaagtccct tctgtcggag
      601 aaactttgga aaaatatagc agatggtttg ttgccgttgt ttatttagga ttggggatat
      661 atatcctgat tgaaaacaac agttttgata tgctatggac tgtgtcgggc caggaaaaaa
      721 tattatgaaa aagatagtat ctgcaaagac tgccccgtgc agtctttttt gttgccggtc
      781 tttttgtgtc tgatgccgtc tgaagcagtc tctgcacgac ctttgtgcga atatttgcta
      841 cacttggcaa tccgtccatt tcttctcacg cttatgctga cctatacccc gcccgatgcc
      901 cgcccgcccg ccaaaaccca cgaaaaaccg tggctgctgc tgttgatggc gtttgcctgg
      961 ctgtggcccg gcgtgttttc ccacgatttg tggaatcctg ccgaacctgc cgtctatacc
     1021 gccgtcgaag cactggcagg cagccccacc cccttggttg cccatctgtt cggtcaaacc
     1081 gatttcggca taccgcccgt gtatctttgg gttgccgccg cattcaaaca tttgctgtcg
     1141 ccgtgggcag ccgacccgta tgatgccgca cgctttgcag gcgtattttt tgccgttatc
     1201 ggactgactt cttgcggctt tgccggtttc aactttttgg gcagacacca cgggcgcagc
     1261 gttgttttaa tccatatcgg ctgtatcggg ctgattccgg ttgcccattt cctcaatccc
     1321 gccgccgccg cctttgccgc cgccggactg gtgctgcacg gctactcgct ggcacgccgg
     1381 cgcgtgattg ccgcctcttt cctgctcggt acgggttgga cgttgatgtc gctggcggca
     1441 gcttatccgg cggcgtttgc gctgatgctg cccctgcccg tgctgatgtt tttccgtccg
     1501 tggcaaagca ggcgtttgat gttgacggca gtcgcctcgc ttgcctttgc cctgccgctt
     1561 atgaccgttt acccgctgct cttggcaaaa acgcagcccg cgctgtttgc gcaatggctc
     1621 aactatcacg ttttcggtac gttcggcggc gtgcggcaca ttcagacggc attcagtttg
     1681 ttttactatc tgaaaaatct gctttggttc gcactgcccg cgctgccgct ggcggtttgg
     1741 acggtttgcc gcacacgcct gttttcgacc gactggggga ttttgggcat tgtctggatg
     1801 cttgccgttt tggtgctgct cgcctttaat ccgcagcgtt ttcaagacaa cctcgtctgg
     1861 ctgctgccgc cgcttgccct gttcggcgcg gcgcaactgg acagcctgag gcgcggcgcg
     1921 gcggcttttg tcaactggtt cggcattatg gcgttcgggc tgtttgccgt gttcctgtgg
     1981 acgggctttt tcgccatgaa ttacggctgg cccgccaagc ttgccgaacg cgccgcctac
     2041 ttcagcccgt attacgttcc cgacatcgat cccattccga tggcggttgc cgtactgttc
     2101 acacccttgt ggctgtgggc gattacccgg aaaaacatac gcggcaggca ggcggttacc
     2161 aactgggcgg caggcgttac cctgacctgg gctttgctga tgacgctgtt cctgccgtgg
     2221 ctggacgcgg cgaaaagcca cgcgcccgtc gtccggagta tggaggcatc gttttccccg
     2281 gaattaaaac gggagctttc agacggcatc gagtgtatcg gcataggcgg cggcgacctg
     2341 cacacgcgga ttgtttggac gcagtacggc acattgccgc accgcgtcgg cgatgtccgt
     2401 tgccgctacc gtatcgtccg cctgccccaa aacgcggatg cgccgcaagg ctggcagacg
     2461 gtctggcagg gtgcgcgccc gcgcaacaaa gacagtaagt ttgcactgat acggaaaatc
     2521 ggggaaaata tattaaaaac aacagattga gccgaatttc tggattaagt gccggaaatc
     2581 gcgtataatt gcgcgattaa accttt
//
LOCUS       DAXDSD010000150         2599 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXDSD010000150 DAXDSD010000000
VERSION     DAXDSD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.150"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            115..522
                     /locus_tag="ACFB2Q_002026"
     CDS             115..522
                     /locus_tag="ACFB2Q_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918980.1"
                     /translation="MNQQEFEFMNDLARAFERRYRDTRSLNRCLSIEGRYMGEEACPH
                     KPEIGLRYGEDAMFLTLQAWAKVDAPQQEAVRISFGIGAKSQAAYEERLQAEIRRRGE
                     GPLHLQTDLGLAAWYRAIRQAAGNDFDLLFEKV"
     gene            613..1773
                     /locus_tag="ACFB2Q_002027"
     CDS             613..1773
                     /locus_tag="ACFB2Q_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC0918981.1"
                     /translation="MNAAAVSAVFKERIASHAEHVKKVAHICTTEETTKQALILPLLD
                     ILGFSAFDPNKVRAEYQADFPGAKSGERVDYALFCNGAPVMFIEAKSYTENLSNHCPQ
                     LSRYFNATPEIAICAITNGREWRFFTDLSNKNIMDSEPFLTVDVTMLNENDMAQLYQF
                     RHDKFQPDALRSLAEESIYLTAFTESITESLKEVDLDFVRYVAGRANIQRQFTQRYLE
                     SIRHIVKQAVQNTVSSMVVSGLSAPKVQEEAAPVEKEQEDPTAPIIDPENNKIVTTYS
                     ERRLFDLVKSILPDDASIEAKDTESYFGVLVDGKSNRWILRYFDNKQRPSVIFPIELE
                     ESDISNIERCGLEVSGNQVIIDTPENLLRVAWLVIDSYRFCCDDENFKRKPK"
     gene            2055..>2599
                     /locus_tag="ACFB2Q_002028"
     CDS             2055..>2599
                     /locus_tag="ACFB2Q_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC0918982.1"
                     /translation="MNVIQSFNFNTTSPVRAFEKDGLTWFVAADVCKALEIQNPTQAL
                     EKLDDDERSMFNIGRSEIHGGGGNVNIINESGLYILILRSRKAMEQGSTAWKFRKWVT
                     SEVLPTIRKTGGYQVGQKTTADDRTGLRQAVAALVGRKGIDYSSAYSMIHQRFNVESV
                     EGIPAGKLPEAVAYVHALTLHT"
BASE COUNT          697 a          550 c          673 g          679 t
ORIGIN      
        1 ttttgatgta tagaaccgct tggcggattg tgcggaaggg gaacttcaga cggccttcgc
       61 ggaaggtgta atgcggactt tggttttgtt gcatgaaaaa gggttaatca agctatgaat
      121 cagcaagaat ttgaatttat gaacgacttg gcgcgtgctt ttgagcgtcg ttaccgtgat
      181 acgcgcagcc taaatagatg tttgagtatc gaaggccgtt atatggggga ggaagcttgc
      241 ccgcataagc ctgaaatcgg cttgagatac ggcgaagatg ccatgtttct tactttgcag
      301 gcatgggcga aggtggatgc gccgcaacaa gaggccgtcc gtatttcgtt cggcatcggt
      361 gcgaagtcgc aggcagccta cgaggaacgt ttgcaggctg aaatcaggcg gcgcggcgag
      421 gggcccctgc atttgcagac ggatttgggc ttggccgcgt ggtatagggc gatacggcag
      481 gcggcaggga atgattttga tttgctgttt gagaaggttt gattttcttt tcggttgcgc
      541 tatgattgcg aaaattaaca cttcttaatg gggtgggaat ttgcccaatc aaatttaacc
      601 aaggagatta acatgaatgc ggcggctgta agtgcggtat ttaaagaacg gattgcttcc
      661 catgcggaac atgtcaagaa ggttgcacat atctgtacta ccgaggagac gacaaaacag
      721 gctttgattt tgcctttatt ggatattcta ggtttttcgg cattcgatcc gaataaggtt
      781 cgggcggaat accaggcgga tttcccaggt gcaaaatcag gggagcgggt cgattacgca
      841 ttgttttgca acggcgcacc ggttatgttt atcgaagcaa aatcatatac tgaaaatttg
      901 tccaatcatt gtccgcagtt gtcgcgttac ttcaatgcca cgcctgaaat agccatttgc
      961 gcgattacca acggccggga atggcgattt tttaccgatt tgtccaacaa gaatattatg
     1021 gattcggagc cgtttttgac cgttgatgtt acgatgttga atgaaaatga tatggcgcaa
     1081 ctgtatcagt tcaggcacga caagtttcaa cctgatgcat tgcgaagcct tgcggaagaa
     1141 agcatatacc tgacggcatt tacagaatca attacagaaa gcttgaaaga ggtggatttg
     1201 gatttcgtcc gttatgtggc gggaagggcg aatattcaac ggcagtttac ccaacgatat
     1261 ttggaaagca tccgtcatat cgtcaaacag gctgttcaaa atacggttag ttcaatggtt
     1321 gtatcgggac tgagtgcgcc gaaagtgcag gaggaggccg cccctgttga gaaggagcag
     1381 gaagacccga ccgcgccgat tatcgacccc gaaaacaata aaatcgttac cacttattca
     1441 gaaagaaggt tgtttgattt ggttaaatca attcttcctg atgatgcgag tattgaggca
     1501 aaagatacgg aaagctattt cggcgtattg gttgatggta aaagtaacag gtggatatta
     1561 cggtattttg acaacaaaca aaggccgtct gtaatcttcc ctatcgaatt ggaagagtcg
     1621 gatatttcga atatcgaacg atgcggactt gaggtatcag gcaatcaagt tattattgat
     1681 acgcctgaaa atctgcttcg cgttgcttgg ttggttattg attcttaccg attctgttgt
     1741 gatgacgaga attttaaacg caaacccaaa taaggtaaca aaaccccttg catgtgcagg
     1801 ggggtttgtt ttatattcct gttcgtggcg taagaaccac acactagcgg caatcactcc
     1861 gtcaatgtga ttttttcata tctgtaagtt tcctttcgtg ttgtttgttt cgatggcaca
     1921 accgaagttt cttatggacg cgtgggcgac gaatacaata cccgtaaggg gaataagtcc
     1981 gcccatctag tgagggttct taaccacgcg tccgccctaa cgggcattaa gaaaactcac
     2041 taggaaactt cattatgaac gtaattcaat catttaattt caatactact tctcccgttc
     2101 gtgcctttga aaaagacggc ttgacttggt ttgttgccgc cgatgtatgt aaggctttag
     2161 aaatccagaa cccaacgcaa gcacttgaaa aattagatga tgacgagcgg tctatgttta
     2221 acatagggcg gtctgaaatt cacggaggcg gcggtaacgt gaacatcatc aacgaaagcg
     2281 gtttatatat tttgattctt cgcagccgta aagcaatgga acaaggctca accgcttgga
     2341 aattccgcaa atgggtaaca tccgaagtcc tgcccaccat ccgcaaaaca ggcggctacc
     2401 aagtcggaca aaaaaccacc gccgacgacc gtaccggatt gcgccaagcc gttgccgcac
     2461 ttgtcggacg caaaggcata gactactcct cagcgtacag tatgattcac caacgcttca
     2521 acgtcgaatc cgtcgaaggc atccccgccg ggaagctgcc cgaagccgtc gcctacgtcc
     2581 acgcgctgac cctgcacac
//
LOCUS       DAXDSD010000151         2596 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXDSD010000151 DAXDSD010000000
VERSION     DAXDSD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.151"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1173
                     /gene="lpxB"
                     /locus_tag="ACFB2Q_002029"
     CDS             <1..1173
                     /gene="lpxB"
                     /locus_tag="ACFB2Q_002029"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC0918983.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARL
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
     gene            1181..1339
                     /locus_tag="ACFB2Q_002030"
     CDS             1181..1339
                     /locus_tag="ACFB2Q_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918984.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            1430..1699
                     /locus_tag="ACFB2Q_002031"
                     /pseudo
     CDS             1430..1699
                     /locus_tag="ACFB2Q_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1813..>2596)
                     /gene="dapB"
                     /locus_tag="ACFB2Q_002032"
     CDS             complement(1813..>2596)
                     /gene="dapB"
                     /locus_tag="ACFB2Q_002032"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC0918985.1"
                     /translation="GANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALGLDAGYAVGL
                     KTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTGFDDAGKAAI
                     RAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHKVDAPSGTAL
                     RMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHTALFATDGER
                     VEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
BASE COUNT          560 a          577 c          827 g          632 t
ORIGIN      
        1 atgtggggac agacgaatat ggttgataaa aaaagccctt tgattgccgt cagtgtcggc
       61 gaagcgtcgg gcgacctgtt gggggcgcac ctgatacgcg ccatccgaaa gcgttgcccg
      121 caggcacggt taaccggcat cggcggggaa ctgatgaagg cggaaggttt cgagagcctt
      181 tatgatcagg agcggctggc ggtgcgcggc tttgtcgaag tggtcaggcg gctgccggaa
      241 attttacgga tacgcaggga gctggtacgg gatttgctgt cgttgaaacc tgatgtcttt
      301 gtcggtatcg atgcgcccga ttttaatctg ggtgtggcgg aaaagctgaa acgggcgggc
      361 attccgaccc tgcattatgt cagtccgtcg gtttgggcgt ggcggcgcga acgcgtgggc
      421 aaaatcgtgc atcaggtcaa ccgcgtgctg tgcctgttcc cgatggagcc gcagctttat
      481 ctcgatgcgg gcggacgtgc ggagtttgtc ggtcatccga tggcacagct tatgcccttg
      541 gaagacgacc gtgaaacggc gcggaaaact ttgggcgcgg atgtcggcat ccccgtattc
      601 gccctgctgc ccggcagccg cgtcagcgaa atcgactata tggcgccggt gttttttcag
      661 acggcattat tgttgttgga acgctatccc gccgcccgct tcctgctgcc tgccgcaacg
      721 gaggcgacga agcggcgttt ggcggaagtt ttgcagcggc cggagtttgc cggattggcg
      781 ctgacggtaa ccgacagaca gtctgaaacg gtgtgcaggg cggcggatgc ggtgctggta
      841 acgagcggta cggcgacttt ggaggtggcg ttgtgcaagc gtccgatggt catcagctac
      901 aagatttcgc cgctgaccta tgcttatgtg aaacgcaaaa tcaaagtgcc gcatgtcggc
      961 ctgccgaata tcctgttggg taaggaggcc gtgccggaat tattgcaatc tgaagcaaaa
     1021 ccggaaaaac tggcggcggc attggcggac tggtacgaac accccgataa ggttgccgcg
     1081 ctgcaacagg atttcggggc gttgcacctg ctgttgaaaa aagatacggc ggatttggcc
     1141 gcgcgtgcgg ttttggaaga ggcgggatgt tgagcggtta atggattatt ttcccgaagc
     1201 agcacgtatt acaaaaaaag ggggagaaat tgtgattaat ggcacatcaa acaataagta
     1261 tttaagagga attccaaatg aaacagaact ggcccgaatg ggattaaggt taaaatataa
     1321 tggttcgtta actgattaat tttattatat atgattatag tttatactaa tacgcttacg
     1381 taccttgttt catttgttct tcgtaaattt ctattttagg caattgtgtc ctacaccaaa
     1441 ttacacagac tgggtaaaaa ttaaattcaa gcagttcagc tatttgaaat ttatctatgg
     1501 atacgctacg gagaaccaag ataaagatat cgataatacc ttggagcttg gagaattaaa
     1561 gcaggatgat gaaatcttgg attatggagg tgcactggca ttaataaggc gtaggtataa
     1621 tcttccgacc agttttatca tagatatagt ttgccgggaa atagagttgg aatttttaga
     1681 tcaggagagt ttcaattaaa cgagccgtag cttgtcatgc tgcacaagca actttattga
     1741 tatgccgata cgaagcctgt cggcaaaatg ccgtctgaac aatatctttt cagacggcat
     1801 tttgtatggg ggttaacggt tgttcagccc gagtacgtcc tgcatatcgt acaaacccgt
     1861 tttgccgttt acccaaactg cggcgcggac ggcaccggcg gcaaaggtca tgcggctgcc
     1921 ggctttgtgg gtgatttcca cgcgttcgcc gtcggtggcg aagagggcgg tgtggtcgcc
     1981 gactatgtcg cctgcgcgga cggtggcaaa gccgatggtg gaaggatcgc gcggaccagt
     2041 gtggccttcg cggccgtaaa cggcgcattg tttgaggtcg cggccgagcg cgccggcgat
     2101 gacttcgccc attcgtaatg ctgtgccgct gggggcatcg actttgtggc ggtggtggcc
     2161 ttcgatgatt tcgatgtcgt agccttcgtt gaggacgcgg gcgacggtgt cgaggatgtg
     2221 gaaggtgagg ttgacgccga cgctgaagtt ggcggcgaaa acgatgcctg ttttttcggc
     2281 ggcggcgcgg atggcggctt tgcccgcatc gtcgaagcct gttgtgccga tgatgatgtt
     2341 gacttgtttt tcaacgcatt tttgcaggtg cttgagggtc ggctcggggc gggtgaagtc
     2401 gatgagtacg tcgctttggg cgagaacggc gtcaacgtcg tctgaaatgg cgatgccggt
     2461 tttgagtccg acggcgtagc ccgcgtccag cccgagggct tctgagcctg agtgttcgag
     2521 cgcgccggaa aggacggtgt cgggatggtt gttgacggct tcgacgagga cgcgtcccat
     2581 gcggccgttt gcgccg
//
LOCUS       DAXDSD010000152         2575 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXDSD010000152 DAXDSD010000000
VERSION     DAXDSD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.152"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..515)
                     /locus_tag="ACFB2Q_002033"
     CDS             complement(<1..515)
                     /locus_tag="ACFB2Q_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFC0918986.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVSALVGRKGIDYSSAYSMVHQRFNVESVEGIPAGKLPE
                     AVAYVHALTLHT"
     gene            complement(1014..1190)
                     /locus_tag="ACFB2Q_002034"
     CDS             complement(1014..1190)
                     /locus_tag="ACFB2Q_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918987.1"
                     /translation="MNPTKQSKKSYESKRVLKHVSFNTEKEANLLEFSNNLDFSKWVK
                     EKLKHELELEKLKK"
     gene            complement(1518..2009)
                     /gene="dtd"
                     /locus_tag="ACFB2Q_002035"
     CDS             complement(1518..2009)
                     /gene="dtd"
                     /locus_tag="ACFB2Q_002035"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC0918988.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(2083..>2575)
                     /locus_tag="ACFB2Q_002036"
     CDS             complement(2083..>2575)
                     /locus_tag="ACFB2Q_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC0918989.1"
                     /translation="FKIAEASFYAEEYVRFLERSETVSESALRQFYERQIRMIKLQQV
                     SFATEEEARQAQQLLLKGLSFEGLMKRYPNDEQAFDGFIMAQQLPEPLASQFAGMNRG
                     DVTRNPVKLGERYYLFKLGAVGKNPDAQPFELVRNQLEQGLRQEKARLKIDALLEENG
                     VKP"
BASE COUNT          615 a          611 c          625 g          724 t
ORIGIN      
        1 gtgtgcagtg tcaatgcgtg gacgtaggcg acggcttcgg gcagcttccc ggcggggatg
       61 ccttcgacgg attcgacgtt gaagcgttgg tgtaccatac tgtacgcgga ggagtagtct
      121 atgcctttgc gcccgacaag cgcggagacg gcccggcgca atccggtacg gtcgtcggcg
      181 gtggttttgg gagtaatttg gtagccgcct gttttgcgga tttgagggat aacctcttca
      241 aatatccagt cttggaattt gacggcttcg gctttgcgtg aacggaagat gactcggtag
      301 agattcggct cgttgatgaa aagtaattct tggttgcctt tttttgttgc aacgctggat
      361 ttttgaatgc cctggtcttt taatggcaac tgacgggcat tttgaatttg aagaatttcc
      421 gcaacatcgc caaggcaaaa ccaaggttca gcccccaaga tttgagtacg gacattttga
      481 gatttgaaag aaaatacaga aatggtgttt tgcattttaa gctccacgaa agtttgaaag
      541 cctctcttcg accaagaaga ggtaggcgga cgtttcaggt tggtcgactg ctcgtggaaa
      601 cagcacatcc ggagatgtcc cgaaagtccg cccgttggct ttttcagcaa gggtgtatgt
      661 ttaacataca cccttcaggg aactcttatc aaaacaaaag ccgcaagcgc ggcaataacc
      721 acgaaagaaa caggcgacca aacctgtgaa ccgaacttta ctattattcc gctattgcgt
      781 caatattttg gggggaagca cggacggagc tttgttgaaa gtttaaaact tggacagtat
      841 tcatggtaga atgacctttc tatttcatgg tagatagacg cggaaagaga gttgccgctc
      901 tgctttccac ctgcccaaat caagttacag cttggtttgg gcttcttttt tacctgcaat
      961 cagcaagtgt tgaaattata atttgtatct acgattcatg caagccattt ctattatttt
     1021 ttcagttttt ctaattcaag ttcatgtttc agcttttctt ttacccattt ggagaagtct
     1081 aaattattgg aaaattccaa taaattagct tctttttcag tgttaaatga aacgtgcttt
     1141 aaaacgcgct tagattcgta gctttttttg gattgtttgg ttgggttcat tcttccccct
     1201 tgttgaaaat aatcgtagat acattttact cgatttaaaa tcatagggca acaaaaaaac
     1261 cgccaagttt gcggcttggc ggcggtcggg ctattttaag aactcttttc ggattttttc
     1321 tttaacccat tgggaaaaac taattttatt tgcaccttat ttgcaccttt tttataaata
     1381 taattcaaat atttgattta tatattaatt taattaaata ttacatgacc ctgcataggg
     1441 gcgacggaaa ggcggcgcgg gggaagggtg tgtgtattgt cgttcattgt cgatatttca
     1501 tcattgcgga ctcaatttca atccggaaca accttcattt ttggggaaat ccgcgtcatg
     1561 aaagagtcca gcagtatggt taccggcccg tcgttgcaga gcgacacctg catatgcgtg
     1621 cggaaacgcc ctgtttcgac atgaatcccg tgtccgcgta acagctccgc cgttcgcagg
     1681 taaagccgtt gcgcctgttc tgccggcgcg gcttgggaaa acgaaggccg ccgcccgctt
     1741 gccgcgtcgg cataaagcgt aaactgcgac accagcagca ccgcgccgcc gacatctttc
     1801 aaagacaggt tcagcttgcc cgcttcgtct tcaaacacgc gcaaatgtgc gattttgtcg
     1861 gcgatatagc gtgcatcttt ttctgtgtcg ctatgcgtta caccgagtaa cacgacaaac
     1921 ccgccgtcga ttttgccgca ggtttccgtg ccggcttcgg acacgacatc cacctttgca
     1981 cctaccgttt tctgtatgac cgcacgcata agccgtcctc cttacctgaa cgtgcaatgc
     2041 cgtctgaaaa ggcttcagac ggcatcggta tcggaaatgc cattacggtt tgacaccgtt
     2101 ttcttccaaa agggcatcga ttttcaagcg ggctttttcc tgcctcaaac cttgttccaa
     2161 ctggtttctg accaactcga aaggctgcgc gtcggggttt ttcccgaccg cgccgagttt
     2221 gaacaggtaa tagcgttcgc ccaatttgac cggattgcgg gtaacgtcgc cacggttcat
     2281 accggcaaac tgcgaagcca gcggctcggg aagctgctgc gccataatga aaccgtcgaa
     2341 cgcctgctcg tcgttcggat aacgcttcat cagcccttca aaagacagcc ctttgagcag
     2401 gagctgctgc gcctgacgcg cctcctcttc ggttgcgaag ctgacctgct gcaatttgat
     2461 catgcggatt tgccgctcat aaaactgacg cagtgcgctt tcggaaaccg tttccgaacg
     2521 ttccagaaaa cggacgtact cctcggcata aaaagacgct tcggcgattt taaag
//
LOCUS       DAXDSD010000153         2444 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXDSD010000153 DAXDSD010000000
VERSION     DAXDSD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.153"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(76..495)
                     /locus_tag="ACFB2Q_002037"
     CDS             complement(76..495)
                     /locus_tag="ACFB2Q_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918990.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            complement(526..2412)
                     /gene="mafB"
                     /locus_tag="ACFB2Q_002038"
     CDS             complement(526..2412)
                     /gene="mafB"
                     /locus_tag="ACFB2Q_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC0918991.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDPFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEVKTKKGG
                     KVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSGIKIEGFTEPNRTAY
                     PIYE"
BASE COUNT          561 a          599 c          541 g          743 t
ORIGIN      
        1 atcatggcac agcgtataag cgtggatttg cagtccaatc tatatcgctg attctattca
       61 ttaagctaat tatgcttatt cctcaatcac agtctccacc accgaatgaa tacttttagg
      121 caattgcaaa aaagcattcc aagcaattaa agcagtttta aaagtatgca gcgaacagct
      181 taatactgga taaccatctt cttcatcaaa ttcattgtgg taaaaaatga ccttatcatg
      241 atgaaattct aggcaaaatt gttgaccacc gccatcatca taaatttcat ttggattatt
      301 ttctattaat tctataatct tgaaatgctc atcaatcgtg gatcgatagt aaatatcgct
      361 cattaaggta gacgtaatgt aatcatatat tcctacataa taatcaaccc tattatcata
      421 gtcatccaga atgatacaca ttctaaatgt atctcctgat ggaaatttat cacaaaagca
      481 cttaattctt ttcatcatta atctcctaat ttttcataaa tattactatt cataaatggg
      541 atatgctgtt ctattaggtt cggtaaatcc ttctatttta atacccgatt tacttgtacc
      601 ctgccattta ttatccttaa gcattattct actttcccaa gccgaagtaa cttcagccct
      661 aattctagcc tcatcccaat cttttgggaa catggtgtgc ttggtcatca ctttcccacc
      721 ttttttcgtt ttcacctccc aacttccatc aggcttttta atttccactg tcgcttgata
      781 aaccccatgt ttatcaggtg ccgaggtttg ttgtatcacc cgtacatcac cacgggttag
      841 actatgccct cctgttactg tgcctttctt attgatatca ccacctataa aatgattaaa
      901 atctaaccct gttttatttt cccattctcg ttgcgcatgg gctttaaact gactactctg
      961 actccttctt ctcgtttcct gaacattttt ctccacctgc tcacgagttg tcaatttatt
     1021 aagcttccta tcaacctccc atctcggttt cgcatcaaac acaggcttag ccttaggtat
     1081 accgcccccc gataattctt gaatatcgag cttcgtatca tatttcacgt gcttctcaaa
     1141 attcggaaac cctttaccgt caaacggtac gcctgtcttc gggtggcgtt ggtctgccag
     1201 tttgacattt ttgccgtttg acggcggcac ggttgaggag gtgatgtttt ctttgccgta
     1261 acgctgctcc aagtttgaac ggatatttcg ggaatggtaa ggggacgggt atttggcgta
     1321 tgccgcatcg gcaaaattgt cgctgacggc ggatttccct ttcggcaatg cgatcgcgcc
     1381 catctgcgac cgcttgacag gatgtgccgt gatgccgccc aagccgtatt ttccccggac
     1441 agctccaatc cctttgatgg ggatggctgc cataaagata ttgctgacgg cttctatgcc
     1501 ttgtgcggca ttggggtttt ggactgccca atcgcggatg gctgctgcgg catagtcttt
     1561 gagttgcgcc atatctgcca aatcgttgat gcgcgccatc ttgttttcgg tggaaagcag
     1621 acccaagccg tgcatgacag caatgtttga gccttcgctt ataccctgca cggcatcgcc
     1681 tgcgccgaca atttctcctg ccgcgccgat gatgtttttg acgatatctg cagtgccgtt
     1741 gaaggcttcg gcggcattgc ccgatctgtc cagctcgggg ctgtatcggg tggcgcgttt
     1801 gaatccgtcg cctactcctt gcgtcagcat agcgccggca ttgtggaaac ggtcggcaag
     1861 ccgttgtccg gtgctgcggt tgtcggtcag gttgaggcgg atattttggg caacgccttt
     1921 tatgtcgtag ctgtatatat ccctcgcgcc tttgggagcg ggatagccgc cgccctgtgg
     1981 cccgtcatag ccgtcggcgg gatggtgttc gtatccgtcc caatggatgc ggtaaaggct
     2041 gaatccgtca acgggactac cggcttcgtc agaatcggaa tgtgaggcat ggttgtcgaa
     2101 gggggaatgg acttcgtgcc cgtgatcgga aaagcggaca atgtagccga tatttccttt
     2161 aatggccgcc tgttgaatca tcaggttgcc caactgatgg ctttgtatgt ttcccaatcc
     2221 gatatggccg ctgcgctcgg caagctcccc cctgctgccg aataggtggt atttcccgtc
     2281 gggttcgaaa tgctgacggt cgagaacctg ccggataaag ggatcgtttg ccaaatctga
     2341 ggcgtgtgca tgcatcggca ggcacactgc cagtatggac agaataaggg atattttgcg
     2401 ggaaatgccc aatttgatgt tccttaagat ggtacggaat gaaa
//
LOCUS       DAXDSD010000154         2410 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXDSD010000154 DAXDSD010000000
VERSION     DAXDSD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.154"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(161..721)
                     /locus_tag="ACFB2Q_002039"
     CDS             complement(161..721)
                     /locus_tag="ACFB2Q_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918992.1"
                     /translation="MDIKTEEAYRLSFQHIQSHDVLAACRTVANYELSQPRPRGLFSG
                     ISAQEYWESYPCNEDAEILKSILLDSPEILDGISGGDMEMARIMAAFNFIWGLSHFPK
                     WLHRHEFASTPLDDSAVPAMLLFHAQSRQELKEYEEVELSCCPDGCEFCKSEDGKIYK
                     SSDAPVLPHAHCTHEQGCRCCYLPVI"
     gene            complement(721..1419)
                     /locus_tag="ACFB2Q_002040"
     CDS             complement(721..1419)
                     /locus_tag="ACFB2Q_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFC0918993.1"
                     /translation="MNRVDRIKNLIADRFNGNQAEFSRAINKAPAQINQWLNGYRNIG
                     DGVAAQIEIALGLPRGWVDGNDKPGIPVDSIKSNATVIGSVDAWDSKTPLSDDDCEVP
                     FYKDVCLSAGNGFSDEIEDYNGYKLRFSKSTLRRHGINPDDVVCVSADGDSMEPVFPD
                     GATLGINTADKMIKDGKIYAINHSGLLRTKILQKLPDNQVRIKSYNPEYKDETASLDS
                     LTVIGRVFWWSVLD"
     gene            1518..1763
                     /locus_tag="ACFB2Q_002041"
     CDS             1518..1763
                     /locus_tag="ACFB2Q_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC0918994.1"
                     /translation="MTEIEMDLRDYCAIRGNQSDLARKTGISPSFIHQIAKGLKPVPI
                     QSAALIEKSTNGRVTRKEMFPDTWHLIWPELADDQPK"
     gene            1866..>2410
                     /locus_tag="ACFB2Q_002042"
     CDS             1866..>2410
                     /locus_tag="ACFB2Q_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFC0918995.1"
                     /translation="MKYIPNSFQIANAVVDDFLCRMSGNAWKCYAVIVRKTTGWQKEI
                     DYISVSQFKNLTGIKTDVTVADALKELVELNLIASVKRHGQVTGYRINMPEPSPENGG
                     TPPPENGGTATPKNWVHPKNGTTPKNWGVLPPENGGTTTPKNWGSTKHTTKPTNTKHS
                     ISASAAADAPLSAEPPESGKRA"
BASE COUNT          668 a          585 c          549 g          608 t
ORIGIN      
        1 tcgggcggct tttttacgaa cataaaaaaa ttataaagtc aaggaagcac catgttgact
       61 tcctgaaatc accatgccga tttcctaaca ccggcgggca agcctacatg atgttcattt
      121 tttgggtatt aaaaaagccc gcatcgcgcg ggctgtgttt ttaaataaca ggcagatagc
      181 aacatcggca accctgctca tgcgtgcagt gtgcgtgtgg gagaacgggt gcgtctgatg
      241 atttgtatat cttgccgtcc tccgatttac aaaattcaca accatcgggg caacaagaca
      301 gctcgacttc ttcgtactct tttaattcct gacgcgattg tgcgtggaaa agcagcattg
      361 cagggacggc agaatcatca agaggcgttg atgcaaattc atgccggtga agccatttcg
      421 ggaaatgact caatccccaa ataaaattga atgcagccat gattcgcgcc atttccatat
      481 cgccgccgct gattccgtcc agtatttcag gggaatctaa aaggatagat ttcaagattt
      541 cagcgtcctc attgcacggg tagctctccc agtattcttg agcggatatg cccgaaaaca
      601 gcccgcgcgg tcttggctgc gataattcat aatttgcaac cgttctacac gccgccaata
      661 cgtcgtgtga ttgaatgtgt tgaaagctca atcgatatgc ttcttctgtt ttaatatcca
      721 ttagtctaat acactccacc aaaataccct gccaataacg gttaggctgt ctaaagaggc
      781 ggtttcgtct ttgtattccg ggttgtagct cttgatacgg acttgattat caggcagctt
      841 ttgcaagatt tttgtacgca acagcccact gtggttgatg gcatagattt tgccgtcttt
      901 aatcatttta tcggcagtat taatacccag tgtagccccg tcggggaata ccggctccat
      961 actgtctccg tcggcagata cgcagacaac atcgtcaggg tttatgccgt gccgtcttaa
     1021 ggttgatttt gagaaacgca gtttatagcc gttgtagtct tcaatttcat ctgaaaagcc
     1081 gttacccgcc gacaaacaaa cgtctttata aaatggaact tcgcaatcat cgtcggataa
     1141 cggggttttg ctatcccaag cgtcaacaga gccgataacg gtggcgtttg atttgatgga
     1201 atcaacaggg atacccggct tgtcgtttcc atccacccat ccgcgcggta atccaagcgc
     1261 gatctcaatt tgggcggcta cgccgtcccc tatatttctg tatccgttaa gccattgatt
     1321 tatctgggca ggagctttat taattgcccg agaaaactca gcttgattgc cgttaaacct
     1381 gtcggctatc agatttttaa ttctatctac tcggttcatt tcattacccc ttcaaatagg
     1441 tataaatata aagcatagct acatttatca atgctactat tttatattta gctatgctat
     1501 actcacgcta aattgatttg acagagatcg aaatggattt aagagattat tgcgcgatac
     1561 gcggcaatca gtctgatttg gcaagaaaaa caggaatttc cccgtctttt atccatcaga
     1621 ttgccaaagg attgaagccc gtcccaattc agtcggcggc gttgattgaa aagtcaacaa
     1681 acggcagggt aacacgaaaa gagatgtttc ctgatacctg gcatctaatc tggccggaat
     1741 tggcagacga ccaacccaaa taaaaagccc gtcggggatg acgggcagcc ggttacgcat
     1801 tactcgattg ttgaaaacgg ggtggaagtg tgggcgcaag accaatggga atggaaggac
     1861 ggtaaatgaa atatattcca aattcgtttc agatagcaaa cgcggtagtg gacgatttcc
     1921 tctgccgaat gagcggcaac gcgtggaaat gctacgccgt catcgtgcgc aaaacgaccg
     1981 gctggcaaaa ggaaattgac tacatctctg tttcccaatt taaaaacctg accggaatca
     2041 aaacagacgt aacagttgcc gacgcgctga aagagcttgt ggaattgaac ctgattgcct
     2101 ccgtcaaacg gcacggtcag gtaaccggct accgcatcaa tatgcccgaa ccgtcccccg
     2161 aaaatggggg tacaccaccc ccggaaaatg ggggtactgc cacccccaaa aattgggtac
     2221 acccaaaaaa tgggactacc cccaaaaatt ggggggtact acccccggaa aatgggggta
     2281 ctaccacccc caaaaattgg gggtctacaa aacacactac aaaacccact aatacaaaac
     2341 acagtattag cgcatccgca gcggcggacg cgcccctttc tgccgaacct cccgaaagcg
     2401 gaaaacgcgc
//
LOCUS       DAXDSD010000155         2407 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXDSD010000155 DAXDSD010000000
VERSION     DAXDSD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.155"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..2283
                     /locus_tag="ACFB2Q_002043"
     CDS             <1..2283
                     /locus_tag="ACFB2Q_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HFC0918996.1"
                     /translation="SNSVEQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKN
                     RGLTQSIALAGRIGGAEALLIRTGRHAGEIRAHEAAGRGVQSFNRLAPVDGGSDYAYF
                     VVEGECPKGYAACKDKPKKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPG
                     FRFENKRHYIGGILERTQQTFDTRDMTVPAFLTKAVFDANQKQAGSLRGNGKYAGNHK
                     YGGLFTSGENNAPVGAEYGTGVFYDETHTKSRYGLEYVYTNADKDAWADYARLSYDRQ
                     GIGLDNHFQQTHCSADGSDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTA
                     KIRHNLSVNLGYDRFGSNLRHQDYYYQSANRAYSLKTPAQNNGKKINPDPNGSEKNPY
                     WVSIGRGNVVTRQICLFGNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYD
                     YRSTHSDDGSVSTGTHRTLSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGD
                     KIKAVKIDPEKSFNKEAGIVFKGDFGNLEASWFDNAYRDLIVRGYEVQIKDGKEQVKG
                     DPAYLNAQSARITGINILGKIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQ
                     SHLFDAIQPSRYVVGSGYDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKA
                     TARRTRPWYIVDVSGYYTVKKHFTLRAGVYNLLNHRYVTWENVRQTAAGAVNQHKNVG
                     VYNRYAAPGRNYTFSLEMKF"
BASE COUNT          607 a          692 c          630 g          478 t
ORIGIN      
        1 tcaaactcgg tcgaacaagg cagcggcgca ttggcgggct cggtcgcatt tcaaaccaaa
       61 accgccgacg atgttatcgg ggaaggcagg cagtggggca ttcagagtaa aaccgcctat
      121 tccggcaaaa accgggggct tacccaatcc atcgcgctgg cggggcgcat cggcggtgcg
      181 gaggctttgc tgatccgcac cggccggcac gcgggggaaa tccgcgccca cgaagccgcc
      241 ggacgcggcg ttcagagctt caacaggctg gcgccggttg atggcggcag tgactatgcc
      301 tattttgtgg tcgaaggaga atgccctaag ggatatgcgg cttgtaaaga caaaccgaaa
      361 aaagatgttg tcggcaaaga cgaacgtcaa acggtttcca cccgagacta cacgggcccc
      421 aaccgcttcc tcgccgatcc gctttcatac gaaagccggt cgtggctgtt ccgcccgggt
      481 tttcgttttg aaaacaaacg gcactacatc ggcggcatac tcgaacgcac gcaacaaact
      541 ttcgacacgc gcgatatgac ggttccggca ttcctgacca aggcggtttt tgatgcaaat
      601 caaaaacagg cgggttcttt gcgcggcaac ggcaaatacg cgggcaacca caaatacggc
      661 ggactgttta ccagcggcga aaacaatgcg ccggtgggcg cggaatacgg tacgggcgtg
      721 ttttacgacg agacgcacac caaaagccgc tacggtttgg aatatgtcta taccaatgcc
      781 gataaggacg cttgggcgga ttatgcccgc ctctcttacg accgtcaggg catcggtttg
      841 gacaaccatt ttcagcagac gcactgttct gccgacggtt cggacaaata ttgccgtccg
      901 agtgccgaca agccgttttc ctattacaaa tccgaccgcg tgatttacgg ggaaagccac
      961 aggctcttgc aggcggcatt caaaaaatcc ttcgataccg ccaaaatccg ccacaacctg
     1021 agcgtgaatc tcggttacga ccgcttcggc tctaatctgc gccatcagga ttattattat
     1081 caaagtgcca accgcgccta ttcgttgaaa acgcccgctc aaaacaacgg caaaaaaatc
     1141 aaccccgacc ccaacggcag cgaaaagaat ccctattggg tcagcatagg caggggaaat
     1201 gtcgttacga ggcaaatctg cctctttggc aacaatactt atacggactg cacgccgcgc
     1261 agcatcaacg gcaaaagcta ttacgcggcg gtccgggaca acgtccgttt gggcaggtgg
     1321 gcggatgtcg gcgcgggctt gcgctacgac taccgcagca cgcattcgga cgacggcagc
     1381 gtttccaccg gcacgcaccg caccctgtcc tggaacgccg gcatcgtcct caaacctgcc
     1441 gactggctgg atttgactta ccgcacctca accggcttcc gcctgccctc gtttgcggaa
     1501 atgtacggct ggcggtcggg cgataaaata aaagccgtca aaatcgatcc ggaaaaatcg
     1561 ttcaacaaag aagccggcat cgtgtttaaa ggcgatttcg gcaacttgga ggcaagttgg
     1621 ttcgacaatg cctaccgcga tttgattgtc cggggttatg aagtgcaaat taaagacggc
     1681 aaagaacaag tcaaaggcga cccggcttac ctcaatgccc aaagcgcgcg gattaccggc
     1741 atcaatattt tgggcaaaat cgattggaac ggcgtatggg ataaattgcc cgaaggttgg
     1801 tattccacat ttgcctataa tcgtgtccgt gtccgcgata ttaaaaaacg cgcagaccgc
     1861 accgatatcc aatcacacct gtttgatgcc atccaaccct cgcgctatgt cgtcggctcg
     1921 ggctatgacc aaccggaagg caaatggggt gtgaacggta tgctgactta ttccaaagcc
     1981 aaggaaatca cagagttgtt gggcagccgg gctttgctca acggcaacag ccgcaataca
     2041 aaagccaccg cgcgccgtac ccgcccttgg tatattgtgg acgtgtccgg ttattacacg
     2101 gttaaaaaac acttcaccct ccgtgcgggc gtgtacaacc tcctcaacca ccgctatgtt
     2161 acttgggaaa atgtgcggca aactgccgcc ggcgcagtca accaacacaa aaatgtcggc
     2221 gtttacaacc gatatgccgc ccccggccgc aactacacat ttagcttgga aatgaagttc
     2281 taaatgcccc ctcgccgcaa atgccgtctg aaaggcttca gacggcattt tttacacaaa
     2341 tttcaccgtt ccccatccct cccgacaata ccgtaatccc gaaacccgtc attcccgcgc
     2401 aggcgtg
//
LOCUS       DAXDSD010000156         2398 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXDSD010000156 DAXDSD010000000
VERSION     DAXDSD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.156"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..192
                     /locus_tag="ACFB2Q_002044"
     CDS             <1..192
                     /locus_tag="ACFB2Q_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0918997.1"
                     /translation="LSELVSCFPQNAALLAGLGRAAALQALPLRLLASLNCGLENFEI
                     RTPIGLLSDYGYFDIVVSS"
     gene            275..1144
                     /locus_tag="ACFB2Q_002045"
     CDS             275..1144
                     /locus_tag="ACFB2Q_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFC0918998.1"
                     /translation="MSQLYTQPDLFLQERIPHKPYCKDFKEAPMLVRSYAAAIKRRYI
                     QVNPPHLRVFMLFDLDYEGAGLAWEDNNLPMPAWAAINRENGGAHLAYALSAPVLTAE
                     YGGRQKALRYLAALEAAYKAKLRGDVGFVSLITKNPEHPHWLTLRGVPDAIRGYDLEY
                     LADFVDLDKFKPYIGRSNVEAVGLSRNCTVFNLVSRWAHKNVLAFKQQGYTVQGWLKE
                     VHYQCMRVNGDFPVPMWEKEVKCISKSIANWVWYKFDIAASNRRFSELQAHRNSLRKT
                     TINAGRTKIITEL"
     gene            1148..1390
                     /locus_tag="ACFB2Q_002046"
     CDS             1148..1390
                     /locus_tag="ACFB2Q_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFC0918999.1"
                     /translation="MAYPKLKTTKRDVTAKELAKRFGCSTRTVFRAWSQSRADYLAEN
                     SISRDKPWEHFGISRATWYRRGKPMPSETDNQSETA"
     gene            1387..1632
                     /locus_tag="ACFB2Q_002047"
     CDS             1387..1632
                     /locus_tag="ACFB2Q_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919000.1"
                     /translation="MTRYAINRDALYSAFKDFLYSEINANPALKSATVDDLADIVLAK
                     KWRIFLPDGIKRTTAEKAAQRVLYMTNQPENQTQQEN"
     gene            1635..1820
                     /locus_tag="ACFB2Q_002048"
     CDS             1635..1820
                     /locus_tag="ACFB2Q_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFC0919001.1"
                     /translation="MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESG
                     EHTQYKLSHIVQDPKAA"
     gene            1820..2098
                     /locus_tag="ACFB2Q_002049"
     CDS             1820..2098
                     /locus_tag="ACFB2Q_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease VapD"
                     /protein_id="HFC0919002.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(2076..2330)
                     /locus_tag="ACFB2Q_002050"
     CDS             complement(2076..2330)
                     /locus_tag="ACFB2Q_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cryptic plasmid protein A"
                     /protein_id="HFC0919003.1"
                     /translation="MITENERDRRTAAWLIETYGAEAVAEAETRIAGARKPYPSNIAK
                     VLGASLPEALKRTENAAARQKLAGLRRMLDGKAVKTSQDL"
BASE COUNT          598 a          633 c          580 g          587 t
ORIGIN      
        1 ctgtcggaac tggtttcatg tttcccccaa aacgccgccc tgctggccgg cttaggtcgc
       61 gctgctgcgc tccaagcttt accgcttcgc ctgctggctt cgctaaattg cggattggaa
      121 aattttgaaa taagaacccc tatcgggctg ttatctgatt atggttattt tgacatagtt
      181 gtatcatctt aaaaacaagc atacaatctg caaatcttag acaaagcaaa acccccgcca
      241 aacgccaatc tgcacggggg tttcgagata caacatgagc caattataca cccaacccga
      301 cctcttcttg caagaacgta tcccacacaa gccatactgc aaagatttca aagaagcgcc
      361 tatgctggtg cgctcttacg ctgccgccat caaacgccgc tacatccaag tcaatccgcc
      421 gcatctgcgt gtgtttatgc tctttgactt ggattacgaa ggggcggggt tggcttggga
      481 agacaataat ctgcctatgc ctgcttgggc ggcaatcaac agggaaaacg gcggcgcaca
      541 ccttgcctat gcgctttccg cgcctgtgct gacggcggaa tacggtggga gacaaaaagc
      601 cctgcgctat cttgctgcac ttgaagcagc atataaggcg aaattgcgcg gtgatgtggg
      661 ctttgtatcg ctgattacga aaaatcccga acatccgcat tggctgacgc tgcgcggcgt
      721 tcctgacgca atcaggggct acgatttgga gtatcttgcg gatttcgtgg atttagacaa
      781 gtttaagccc tatatcggtc gctctaacgt ggaagcggtc ggattaagca gaaattgcac
      841 ggtgtttaac cttgtgagcc gttgggcgca caaaaacgtg ttggcgttca aacagcaggg
      901 ctacacggtg caaggctggc tgaaagaagt gcattaccag tgtatgcggg taaatgggga
      961 tttccctgtc ccgatgtggg aaaaagaagt gaaatgtatc tccaaatcaa tcgctaactg
     1021 ggtttggtac aagtttgata ttgcagccag caatcgacgg ttttcggaat tgcaggctca
     1081 tcggaatagt ttgcggaaaa caaccatcaa tgcaggcaga acaaaaatca tcacggagct
     1141 ttgaaatatg gcctatccaa aactgaaaac caccaagcga gacgttacgg ctaaagaact
     1201 ggcaaaacgc ttcggctgtt ccacaagaac cgtttttcgg gcatggtcgc aatcccgcgc
     1261 cgactaccta gccgaaaact ctatcagccg cgataaaccg tgggaacact tcggcatttc
     1321 ccgcgccact tggtacaggc gcggcaagcc tatgccgtct gaaactgaca accaaagcga
     1381 aacagcatga caagatacgc aataaaccgc gatgctctgt actcggcatt caaagatttt
     1441 ctgtattcgg aaattaacgc aaatcctgct ttgaaaagcg caacggttga tgatcttgca
     1501 gatatagttc tagccaaaaa atggagaatt tttctgcctg acggcatcaa acgcacaaca
     1561 gccgaaaaag ctgcccaacg ggttttgtac atgacaaacc agcctgaaaa ccaaacccaa
     1621 caggagaact aaccatgcaa accactatcc ccaccggttc aatccgcacc tttggcgatt
     1681 acggcgtgat gtacatcgtc ggcacgcctg ccgaacagct tccggacggc gattggctcg
     1741 taaatattga actgccggaa agcggcgaac acacgcaata caaactgtcc cacatcgttc
     1801 aagacccaaa ggctgcctaa tgtacgcgat ttcttttgat ttggtggttg ccgataccgc
     1861 tcaaaaccat ccgaaaggca tttcccaagc ctacgcggat attggataca ccctgcggaa
     1921 attcggcttt acccgcattc agggaagttt gtacacctgc caaaacgaag atatggctaa
     1981 cctgttttca gccatcaacg agctaaaagc tctgccttgg tttccgtctt ctgtccgcga
     2041 tattcgggcg ttccgcattg agcagtggtc tgatttcaca agtcttgtga agtcttaact
     2101 gccttaccgt ccaacatccg ccgcagccct gccagttttt ggcgcgctgc ggcgttttct
     2161 gtgcgtttta gggcttcggg taggctagcc cccaatactt tggcgatatt gctcggatag
     2221 ggctttctcg cgcccgcaat gcgggtttct gcttccgcta cggcttctgc cccgtaggtc
     2281 tctatcagcc atgcggctgt tcgcctgtcg cgttcgttct cggtaatcat cggctcattc
     2341 cccatcccct gctttgggtt cgtttgtgtc gttggcttat cgtttggcta gttgattc
//
LOCUS       DAXDSD010000157         2331 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXDSD010000157 DAXDSD010000000
VERSION     DAXDSD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.157"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            419..931
                     /locus_tag="ACFB2Q_002051"
     CDS             419..931
                     /locus_tag="ACFB2Q_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC0919004.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            1011..2090
                     /gene="aroB"
                     /locus_tag="ACFB2Q_002052"
     CDS             1011..2090
                     /gene="aroB"
                     /locus_tag="ACFB2Q_002052"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC0919005.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMALERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
BASE COUNT          567 a          722 c          592 g          450 t
ORIGIN      
        1 agccgcagac agtacggata gtacggaacc ggttcgcccg gtgcttggac gccttaggga
       61 atcgttccct ttgagctaag gcggggcaac gccgtaccgg tttttgttaa tccgctatag
      121 tattgataaa catattatct tcaatatatt caattggata attgtttacc taagcaaaga
      181 taattgcctt ttcctgacaa attaagtgaa atcaacggat tgtcaaaaca cagcctgaaa
      241 taaaaaacct ccctgatttc ttttattttt ccttaaaatc agaaaagttc gggatggtcg
      301 ggttattttt ccaaacgtac cgccgccctg ccgatttcgt ataaaatccc gccgtaaccc
      361 gacaagcccg aaccctgccg ccccgaaagg cgggggggtc aaacattaag gaaccgtgat
      421 gaaaaacttt aacggcaaac tcatcctcat cggactgatg ggcgcgggca aaactacgct
      481 gggccggcaa atggcgcagc ggctggatta ccgtttttac gacagcgatc acgaaatcgc
      541 cgcagcggcg ggcgttccca tccccaccat atttgaaatg gaaggcgaac agggattccg
      601 ttcgcgcgaa accgccatac tcaaaaaact gattgtcctg ccgcatatcg tcctgtccac
      661 cggcggcggc gcggtgttaa aagaagaaaa ccgcgccctt atccgcaaaa gcggcacggt
      721 cgtttatctg cacgccccgc ccgaaaccct gctcgaacgc acgcgctgcg acaacagccg
      781 ccctttgctg caagttgccg atcctttggc gaaattgcgc gaactctacg ccgcacgcga
      841 ccccgtttac cgccaaaccg ccgactttac cgtagaatcg gcaaactgcc gggaaaccgt
      901 gcaaaccctg ctcaaacgct tatcccgata aaccggcata tgcgccgctc cccgaaaacc
      961 aaaccgcacc ccgcccggcg ggccggcggt tcaaacttta aggaacaaca atgaaaacac
     1021 tgaccgtaca cacgccttcg cacagctacc ccatctttat cggcaacggg ctgctgccgc
     1081 aggcgggaag cctgctcaaa ccgcatttgg gcaaacgcgc cgccatcatc accaacgaaa
     1141 ccgtcgcccc gctctacctc ggcacgcttc agacggcatt ggatgcggca ggcgtatccc
     1201 atttcagcat catcctgccc gacggcgagg cgcacaaaaa ctggcagacg ctcaacctca
     1261 tctttgacgg gctgatgcaa aaccgcgccg aacgtaaaac cacgctgatt gcgctgggcg
     1321 gcggcgtgat cggcgacatg gtcggctttg ccgccgccac ctaccagcgc ggcgcgccgt
     1381 tcatccaaat accgaccacg ctgttgagtc aggtcgactc atcggtgggc ggaaaaaccg
     1441 ccatcaacca cccgctcggc aaaaatatga tcggcgcgtt ttaccagccg caggcggtgc
     1501 ttgcagattt ggacacgctg cacaccctgc ccgcccgcga actgtccgcc ggtatggcgg
     1561 aagtcatcaa atacggcgcg ctcggcgaca tcggcttttt tgaatggctg gaacagcata
     1621 tgcccgaact gatggcactc gaacgcgcgc ccctcaccca agccgtgtac cgctgctgcc
     1681 aaatgaaggc agacatcgtc gcccaagacg aaaccgaaca gggcatacgc gcatggctca
     1741 acctcggaca caccttcgga cacgccgtcg aagcggaaat gggttacggc gtatggctgc
     1801 acggcgaggc cgttgccgcc ggctgcgtgt tggcggcgcg tttgtccgaa caactgggca
     1861 aaacctccgc cgcagatacc gcgcggctcg ccgccctgct cgaagccgcc ggactgccgt
     1921 ccgcaccgcc cgtgtttgcc tttgaaaaat ggctggcaca tatgagccac gacaaaaaag
     1981 tcagcggcgg catcatgcgc tttatcggtc tgaaccggct gggcgaagcc gtcatcaccg
     2041 aaattaccga cacggacatc ctccggcgca ccctgcaacc gtatctctga tttcctctgc
     2101 cgatgtgttg ccgcgcgggt ttgacgcacg atgatgactt tccatcatca ttctccgcga
     2161 aagcgggcat ccggttattc catattgcag ggatttatcc ggaataacga caacctgtcc
     2221 gccgtcattt ccgcgcagat ggaaatttgg aaattcaaaa tcacaggaat ttatccggaa
     2281 ttatagcgga ttaacaaaaa tcaggacaag gcggcgaagc cgcaggcagt a
//
LOCUS       DAXDSD010000158         2301 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXDSD010000158 DAXDSD010000000
VERSION     DAXDSD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.158"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..765
                     /locus_tag="ACFB2Q_002053"
     CDS             <1..765
                     /locus_tag="ACFB2Q_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HFC0919006.1"
                     /translation="RLPFMAQYDRLGLTRSNTSCHNNTGKQCLWGDTEKQPELLVLGD
                     SHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYASRVFQNWAACRAVYRYAEEHLP
                     RYPKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHKLSSEKQAVYLMADNLASSYN
                     VQRAYILSSRIPGCRQTLRPDDESTLKANARIRELAAKYPNVYIIDAAAYIPADFQIG
                     GLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            827..2122
                     /gene="tyrS"
                     /locus_tag="ACFB2Q_002054"
     CDS             827..2122
                     /gene="tyrS"
                     /locus_tag="ACFB2Q_002054"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC0919007.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVVEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
BASE COUNT          569 a          706 c          563 g          463 t
ORIGIN      
        1 aggctgccgt ttatggcgca atacgaccgc ttggggctga cgcgttccaa cacctcctgc
       61 cacaacaata ccggcaaaca atgcctgtgg ggggatacgg aaaaacagcc ggaactgctg
      121 gttttgggcg actcccacgc cgaccattac aaaacattct tcgatgccgt gggcaaaaaa
      181 gaaaaatggt ccgccactat ggtttccgcc gacgcctgcg cctatgtgga aggctacgcg
      241 tcccgtgtgt tccaaaactg ggccgcctgc cgcgccgttt accgctatgc cgaagaacac
      301 ctgccccggt atccgaaagt ggttttggcg atgcgctggg gcagccagat gcccgaaaac
      361 agccgctccc ttgcctacga tgccggtttt ttccaaaaat tcgaccgtat gctgcacaaa
      421 ctctcatccg aaaaacaagc cgtttacctg atggcggaca acttggcttc gtcttacaac
      481 gtccagcgcg cctatatctt gtcttcacgc ataccgggtt gccgccaaac actgcgcccg
      541 gacgacgaaa gcaccctgaa agccaatgcc cgcatcaggg aattggcagc caaatacccc
      601 aacgtctata ttattgatgc cgccgcctat atccccgcag atttccaaat cggcggattg
      661 ccggtttact cggacaaaga ccacatcaac ccttacggcg gcacagaatt ggcgaagcgt
      721 ttttccgaaa aacaaaggtt tctcgatacg cgccataacc attgattcgc ttaaatttgt
      781 tacaatcggc ggtttgcaaa accgctaatt tttttgaaag agaccgatga gcgtcatcca
      841 agacctgcaa tcgcgcggcc ttatcgcgca aaccaccgac atcgaagcct tagacgcttt
      901 gctgaacgaa caaaaaatcg ccctttactg cggtttcgac ccgacagccg acagcctgca
      961 catcggacac ctgctgcccg tattggcatt gcgccgcttc caacaggcgg ggcatacgcc
     1021 gattgcactg gtgggcggcg cgaccggtat gatcggcgac cccagcttca aagccgccga
     1081 acgcagcttg aattccgccg aaaccgttgc cggctgggta ggaagcatac gcagccaatt
     1141 aacccctttc ttgagctttg aaggcggaaa cgccgccatt atggcgaaca atgccgactg
     1201 gttcggcagc atgaactgcc tcgacttcct gcgcgacatc ggcaagcatt tctccgtcaa
     1261 cgccatgctg aacaaagaat ccgtcaaaca gcgcatcgac cgcgacggcg caggcatttc
     1321 ctttaccgag ttcgcctatt ccctgctgca aggctacgac ttcgccgagt tgaacaaacg
     1381 ccacggcgcg gttttggaaa tcggcggttc cgaccagtgg ggcaacatca ccgccggtat
     1441 cgacctgacc cgccgcctga accaaaaaca agtgttcggt ctgaccctgc ctttggttac
     1501 caaatccgac ggtaccaaat tcggcaaaac cgaaggcggc gcggtgtggc tgaacgcgaa
     1561 aaaaacctcg ccgtaccagt tctaccagtt ctggctgaaa gtcgccgatg ccgatgtgta
     1621 taaattcctg aaatacttta ccttcctgtc catcgaagaa atcggtgtcg tcgaagccaa
     1681 agacaaggca agcggcagca agcccgaagc gcaacgcatc ctcgccgaag aaatgacccg
     1741 cctgattcac ggcgaagaag cccttgccgc cgcgcaacgc atttccgaaa gcctgtttgc
     1801 cgaagaccaa agccggctta ccgaaagcga cttcgagcag ctcgccctcg acggcctgcc
     1861 tgcatttgaa gtttcagacg gcatcaacgc cgtcgaagcc ttggtcaaaa ccggcttggc
     1921 agcgtccaac aaagaagcgc gcggctttgt caatgccaaa gcggttctgc tcaacggcaa
     1981 accggctgaa gccaacaacc ccaaccacgc cgccgaacgc cccgacgatg cctatctgtt
     2041 gataggcgaa tacaaacgtt tcggcaaata caccatcctc cggcgcggca aacgcaacca
     2101 cgcgcttttg gtttggaaat aatccgattg ccgcagaaat gccgtctgaa gctttcagac
     2161 ggcattttta tcaaatgcaa aacaccctgc gcctgccgat atgtcgtcat ttccatgcag
     2221 gcgggaattc aaacttgtcc gcacggaaac ttatcgggca aaacggtttc ttcagttcta
     2281 cgttctagat gcccgctttc g
//
LOCUS       DAXDSD010000159         2297 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXDSD010000159 DAXDSD010000000
VERSION     DAXDSD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.159"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(94..1728)
                     /gene="groL"
                     /locus_tag="ACFB2Q_002055"
     CDS             complement(94..1728)
                     /gene="groL"
                     /locus_tag="ACFB2Q_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC0919008.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            complement(1821..2111)
                     /locus_tag="ACFB2Q_002056"
     CDS             complement(1821..2111)
                     /locus_tag="ACFB2Q_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC0919009.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKAGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT          423 a          626 c          618 g          630 t
ORIGIN      
        1 tttaaggttt gcgccttagc cgcacttatt cgcaaaaaaa ccgcacggcg ttgaccgtgc
       61 ggcttgttgt ctgaaggttt cagacggcat tgcttacatc atgccgccca taccgcccat
      121 tccccccata tcgggcacag ccggtttttc ttcagggatt tcggcaatca tgcagtcggt
      181 cgtcagcatc agaccggcga tagacgcggc gtgttgcagc gcggaacggg ttactttggc
      241 agggtcgagt acgcccattc cgatcatgtc gccgtattcg ccggagcctg cgttgtaacc
      301 gtagttgcct ttgccttcca acactttgtt caccaccacg ctgggttctc cgcctgcgtt
      361 ggcaacgatt tggcgcagcg gagactcaac ggcgcgcaat acgatttgta cgcctgcgtc
      421 ttggtcggca ttgccggtgt gcaggttttc caaagcggca cgggcgcgca acagggctac
      481 gccgccgcct gcaaccacgc cttcttcaac ggctgcgcgg gtagcgtgca gcgcgtcttc
      541 cacgcggtct tttttctctt tcatttcgac ttcggtcgcc gcgccgactt tgatcactgc
      601 cacgcctcct gccagtttgg caacgcgctc ttgcagtttt tctttgtcgt aatcgctggt
      661 cgcggtttcg atttgttggc ggatttcggc aacacgcgct tcgatttggg ctgcgtcgcc
      721 gaagccgtcg atgacggtag tgttttcttt accgatttcg atgcgtttgg cttgacccaa
      781 gtcgtccaaa gtcgcttttt ccaaagacag gccgacttct tcggaaatca ctacgccgcc
      841 ggtcaggatg gcgatgtctt gcagcatcgc tttgcggcgg tcgccgaagc cgggggcttt
      901 gacggcaacg gttttcagga tgccgcggat gttgttcacg accaaagtcg ccaaggcttc
      961 gccttctacg tcttcagcga taatcaacag cggacggctg gctttcgcca cttgttccaa
     1021 cacgggcagc aggtcgcgga tgttgctgat ttttttgtcg aacagcaaaa caaacggatt
     1081 gtccagaccg gcgatttgtt tttccgcgtc gttgatgaag taaggggaca ggtagccgcg
     1141 gtcgaactgc ataccttcaa ccacgtccag ctcgttttcc aaagatttgc cgtcttcaac
     1201 ggtaatcacg ccttctttgc cgactttttc catcgcttcg gcgataatcg cgccgacttg
     1261 ttcgtcggag ttggcggaaa tcgagccgac ttgggcgatt tctttggaag tatcgcaagg
     1321 tttggcgatg tttttcagct cgtcaaccaa agcggcaacg gctttgtcga tgccgcgttt
     1381 cagatcggtc gggttcatgc cggcggtaac gtatttcatg ccttcggcaa cgatggattg
     1441 tgccaatacg gtggcggtag tcgtaccgtc gccggctacg tcgttggttt tggacgcgac
     1501 ttcttttacc atttgcgcgc ccatattttc aaacttgtct ttcagttcga tttctttggc
     1561 gacggttacg ccgtctttgg tgatgtgcgg gccgccgaaa gcgcggtcaa ccaccacgtt
     1621 gcggcctttg gggcccaagg ttacgcgcac ggcgtttgcc aaaatattca cgccgtttac
     1681 cattttttgg cggacttcat tgccgaattg tacgtctttt gctgccattt caattctcca
     1741 aaaatcatta aaactgtctg ataaaaccgt ttatgccgtc tgaaggcggt ttgccgtttc
     1801 agacggcatc gtgtccgtat ttatttttca acgatgccga aaatatcttc ttcgcgcatt
     1861 accaacagct cttcgccgtc ggcttttacg gtttggccgc tgtatttgcc gaagatgatt
     1921 ttgtcgccgg ctttgacatc cagcggacgg cgcgcgccgt ctttgccgat tttgcccgcg
     1981 cccacggcga tgacttcgcc catatcgggt ttttcggcgg ccgcacccgg caggacgatg
     2041 cccgatgcgg ttttttcttc agcttccaag cgtttgacga cgacgcggtc gtgtaaagga
     2101 cggatggtca tatttatgct ccgataaatg gtttgaaaac aatcatctgc ccgaacggtt
     2161 cggacaaatt gaagtggaaa ccgggctgcc gcctaacggc ctgcccgtac aagtcggcaa
     2221 aattagggtg tgtgcgggaa aattcaagtg aggcggaaaa aaatttattt ccgccgtttt
     2281 ttatagtgga ctaaatt
//
LOCUS       DAXDSD010000160         2280 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXDSD010000160 DAXDSD010000000
VERSION     DAXDSD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.160"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            172..366
                     /locus_tag="ACFB2Q_002057"
     CDS             172..366
                     /locus_tag="ACFB2Q_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC0919010.1"
                     /translation="MDSEYLDPQQCADILSVKKRTFLERYAPRPDFPARISVSKKRFW
                     WKKEEVEGWLDRQKEKRPVM"
     gene            complement(422..506)
                     /locus_tag="ACFB2Q_002058"
     tRNA            complement(422..506)
                     /locus_tag="ACFB2Q_002058"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Leu,seq:tag)
     gene            657..1331
                     /locus_tag="ACFB2Q_002059"
     CDS             657..1331
                     /locus_tag="ACFB2Q_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919011.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWCNDEGWKLADYLPCDDVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1324..1917
                     /gene="ribA"
                     /locus_tag="ACFB2Q_002060"
     CDS             1324..1917
                     /gene="ribA"
                     /locus_tag="ACFB2Q_002060"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC0919012.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            2085..>2280
                     /locus_tag="ACFB2Q_002061"
     CDS             2085..>2280
                     /locus_tag="ACFB2Q_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC0919013.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIRVIPCVT"
BASE COUNT          555 a          523 c          650 g          552 t
ORIGIN      
        1 aactgcgcct attgcaacaa accgcctgcc gcttcaaagc cgccctcgaa acggccgcgt
       61 ggaaacacta cgtccgcgcc atcaaagaat ccgagccggt accggatgcc gaagcgcgcc
      121 ggaagcggaa aaaacaggcg gcgtaaatat aattaagttt ggaggtttta aatggatagc
      181 gaatacctag acccgcagca atgcgcagat attttatccg taaaaaagcg tacattttta
      241 gagcggtacg caccgcgtcc ggatttcccc gcgcgaatat ccgtatcgaa gaaacgtttt
      301 tggtggaaaa aagaagaagt cgaaggatgg ctagaccgcc aaaaagaaaa acgcccggtg
      361 atgtgatttg caccacattt gcaccaagtg gtatataaca ttatgaaaga tataaaaatt
      421 ttggtgcgga cggagagact cgaactctca cacctctcgg cgccagaacc taaatctggt
      481 gcgtctacca atttcgccac gtccgcataa gggtggagat tatacagatt ttgtccgatt
      541 gcgcaaggct ttggacgtaa tagttgaggc ttatgctttg cagcggtaaa atccgctatt
      601 cgtccgcctg gcatcggaat cgggcggttt tttgttttta ttgacggaat ttgggtatgc
      661 ctgctgcttt gattaaggat tttctgctga ctcagggttt gaagctgccg cttgacgagg
      721 ttcgggcggc gtatctgacg gcgcagacgg taatggatat ggggatggct tcgattgacc
      781 gttctgtttt gtggtgcaat gatgagggtt ggaaacttgc cgattacctg ccgtgcgatg
      841 atgtccgcga agatgccctg aaacggcttt ttatggcttt ggattcggtg ttttcacgct
      901 cgacaggtgt gcggagcgcg gcggtttatg ccttgatgcc gtctgaaaac gccgccctcc
      961 ggctggtgtg cctgtcccaa cagggcgaag gtttggaaaa catatgggag caggatggaa
     1021 atattaccga tgtttcgctt gcctgccgtt cggcgcaaag cggttggatg aatgttgcct
     1081 cggatgtacg ccgttggttg aatttgggtg agctttcggg agaacgcaat catgcttcgg
     1141 cggcgcaaat ttccattccc gtttgcacgg agagcggcgg tgtattgggc gtggttcatg
     1201 tggaatttga atgcgcagag tgtgcggata cggcggcgca ggcggaatgg gtggctcttg
     1261 ccttggcttt gtccgaacct ttgaagcaac ttttgggcat cactgccgca gaaggagatg
     1321 aaaatgtctg aattattaga ccatgtcgct tcctgccgtc tgccgaccga atggggcgta
     1381 tttacgatgc acggttttga agaggcaaac gggcaggaac acgtcgcgct gaccgtcggc
     1441 aattgttcag acggcaatcc ggtgttgacg cgcatccact ccgaatgtct gacgggcgac
     1501 gcgctgttct cgagaaaatg cgactgcgga ccgcaacttg aagcggcaat gagggcggta
     1561 caggcagagg ggcgcggcat catcgtctat ctgcgtcagg aaggacgcgg catcgggctg
     1621 attaacaaaa tccgcgccta tcatctgcaa gaacaaggta tggataccgt tgaagccaat
     1681 ttggcactcg ggctgcccgt cgatgcccgc gatttccgtt tggcgcaatc tatctacgaa
     1741 tatctgggca tccgctcggt caaactgttg accaacaacc ccgaaaaaat ccaaaccctg
     1801 aaagatgcgg ggattaacgt ggtcgaacgc attcccctgc acgtcgggga aaatctggaa
     1861 aacgagcgtt atctccaaac caaagcagac aagctggggc atttgatgtc ggaataaggc
     1921 aaagttgcag ggaacgggca tcctgcgccg tctttcggga aacaggtttc catagcttga
     1981 taaagcaata agttttatca agttgcaggg tgcggatgca aacgcattgc gagcgcgggt
     2041 ttgaggcata cgcgcaaaca tcttaataca acgtattgat atttatgatt ttctctatca
     2101 tcgtccctat ttacaatgtg gaaaaatacc ttcgctgctg cgtggattcc gtgcttgccg
     2161 aaaattttgc cgattatgaa atgattttgg tcgatgacgg ttcgccggac ggctgcggga
     2221 agatttgcga cgaatatgca ggcaaatatc cgcatataag ggtaatccca tgcgtaacgc
//
LOCUS       DAXDSD010000161         2265 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXDSD010000161 DAXDSD010000000
VERSION     DAXDSD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.161"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            112..336
                     /locus_tag="ACFB2Q_002062"
     CDS             112..336
                     /locus_tag="ACFB2Q_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919014.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            365..1651
                     /locus_tag="ACFB2Q_002063"
     CDS             365..1651
                     /locus_tag="ACFB2Q_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC0919015.1"
                     /translation="MADALADGGAAATAVGCPPRLIGGEQNKTPNPKGAEKTENQEFQ
                     FEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTKVTGKGLVSDTEFVV
                     RYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHYGGQCETMLVELNGT
                     GCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQAMLDHDNGHYDVHNM
                     RPKSECRGTAWRNEDGSGKTFYIGKRENSKFTRVYEKGRQFGDVDSPWVRFEGDIEIP
                     LDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKLFHARNQVGKMVNFL
                     RDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHEEQKAINALNIETLF
                     DDLIEEREYAFPQDRERQHIKDIEYEERQISDFLNN"
     gene            1665..1976
                     /locus_tag="ACFB2Q_002064"
     CDS             1665..1976
                     /locus_tag="ACFB2Q_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919016.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            1990..2193
                     /locus_tag="ACFB2Q_002065"
     CDS             1990..2193
                     /locus_tag="ACFB2Q_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919017.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT          736 a          444 c          534 g          551 t
ORIGIN      
        1 ggagcggata aattctcgca atctaaaacc ttatatattg cgctccataa ggggcgcata
       61 tttttaatcc ttgtaaagcc ccttaactct agtcagtgaa ggggcttaat catgtttatt
      121 ttctatactg tcaatcccga acacgtatat ttccccaaag catacatcat gaaagtgttc
      181 aaggataaag gctacgaatc ccaatgcatc acaacagttt ctttttatat ctgcaatccg
      241 actttgaagc aaaagaccga aaacgaagcg tatgaatacg gcagattgtt tgttaaagaa
      301 ctgatgcata aggaatgcaa ccgtgaaagc ctttgaagat aaagccttgg cggtgtccgc
      361 ggacatggcg gacgcgttag cggacggcgg ggctgcggca accgccgtag gctgcccccc
      421 taggctaata gggggggagc aaaataaaac cccaaatccg aagggtgcgg aaaaaacaga
      481 aaaccaagaa tttcaattcg agtatttcag tcattttgta tcggacggca aaggcagatt
      541 tatagaaata ccgttaaggc gtggcaggga tgacggagcg ttcatagacc aaatcacttt
      601 cacgatacat gaaaattcca tgacgaaagt aaccggaaag ggattggtat cagatacgga
      661 attcgttgtc cggtatagcg agctgcttga agaaatcttg ggattcggca ttaccaaaaa
      721 attaccgttt aaaggaaagt ttttctatca aagctgttat caattcgggc ccgataacgt
      781 cgaatacgga aaggttcatt acggcggcca atgtgaaacg atgttggttg aattgaacgg
      841 tacaggctgc atggctgctt tacccggttg ggaaaaccga ttgtatgaat ttttaagtaa
      901 atgcgtccga cccaaaataa cccgtatcga tgtcgcccat gattttttca acggagagta
      961 cacacccgat caggcaatgt tagaccacga taacggacat tacgacgtac acaatatgcg
     1021 gccaaaaagc gaatgtcgcg gcacggcatg gcgcaatgaa gacggcagtg gaaaaacttt
     1081 ttacatcggc aagcgcgaaa attcaaaatt tacccgtgtt tacgagaaag gcagacagtt
     1141 tggagatgtt gatagccctt gggtcaggtt tgagggggat atagaaatac ccttggatgt
     1201 cctactttat tcggggtcgt atctaggcgg cgcatatccg atttgcaagg agatttttaa
     1261 gacagaagcc aagcgcatgg aagtaaaagt taaaaacgtt aatctgatat tcgacgttaa
     1321 gctttttcat gcaaggaatc aagtcggcaa gatggttaat ttcctccgcg atatagggtg
     1381 ggacgatagc aggatagttg atgagctggt gaaaggcgtt gaaggttatc caaaaggttt
     1441 acagcctgag caatacgatt gcaaaaacca aacgcagaaa ttccagtata tccacgaaga
     1501 acagaaagcc attaacgcat tgaacattga aacactgttt gatgatttga ttgaagagag
     1561 agaatacgca tttcctcaag acagggaacg gcaacacatc aaggatattg agtacgagga
     1621 acgtcaaata tccgattttt taaacaactg aaggaaattg aaaaatgttt gaaacaagcc
     1681 aagtaaccac atataccgcc actttgttgg gcgcaaaaaa attcaaaggc gaaatcgacg
     1741 gtaacaaaat cgattcatgc accgtcttgg tagccagccc catgccgtca aacggcaatg
     1801 ccgtgggctt tacctcggaa agcatgaagt ttggtgacag ccataatttt gaaaaattga
     1861 aaaaccttaa attcccctgt gcggttgatc taaccgttgc catggaatca acaggaaaag
     1921 gcttagtgca gaaattgttg gatttccaag taaagggcgc agcacctaaa gcctaagaaa
     1981 ggctgaatca tgagcagata ccaacagaaa tttatcgtac aggaattgga aaatcacgaa
     2041 ttcatctatc ccgattcgtt tggcgatatt ggatttacgt cgaacatcaa atcagccggt
     2101 aaatatgaca gctatgaaga cgcttttagc tcggcattgg aagagatagg cggggaattt
     2161 gtaattttcg gattctacga aaaagaagat taagtttaag aggctcggca ggcggtctct
     2221 aaaaccttca caaagcccgc aaacacattt ttttaaaaat ttcgt
//
LOCUS       DAXDSD010000162         2200 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXDSD010000162 DAXDSD010000000
VERSION     DAXDSD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.162"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1324
                     /gene="miaB"
                     /locus_tag="ACFB2Q_002066"
     CDS             <1..1324
                     /gene="miaB"
                     /locus_tag="ACFB2Q_002066"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC0919018.1"
                     /translation="KVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADIILF
                     NTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVVFG
                     PQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKYCS
                     FCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDFAT
                     LLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMK
                     RGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVF
                     IYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGISK
                     KDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            1342..1506
                     /locus_tag="ACFB2Q_002067"
     CDS             1342..1506
                     /locus_tag="ACFB2Q_002067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919019.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(1571..>2200)
                     /locus_tag="ACFB2Q_002068"
     CDS             complement(1571..>2200)
                     /locus_tag="ACFB2Q_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HFC0919020.1"
                     /translation="VDRAGIVGADGPTHAGLYDLSFLRCVPNMIVAAPSDENECRLLL
                     STCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKGIIRREGEKTAFIAFGSMVAPAL
                     AVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVTLEENAEQGGAGGAVLEVLAKHG
                     ICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVERRVREWLPDRDAAN"
BASE COUNT          523 a          717 c          554 g          406 t
ORIGIN      
        1 aaaagtattt atccgcacct tcggctgcca gatgaacgag tacgacagcg aaaaaatgct
       61 gtccgtcctt gccgaagaac acggcggcat cgaacaggtt acccaagccg acgaagccga
      121 catcatcttg ttcaacacct gttccgtgcg cgaaaaagca caggaaaaag tgttctccga
      181 cttggggcgc gtccgtccgc tcaaagaaaa aaaccccggc ctcatcatcg gcgttgccgg
      241 ctgcgtcgcc tcgcaagaag gcgaaaacat catcaaacgc gcgccttatg ttgacgtggt
      301 tttcggcccg caaacgctgc accgcctgcc caaaatgatt gtggacaaag aaaccagcgg
      361 gctgtcgcaa gtcgatattt ccttccccga aatcgaaaaa ttcgaccacc tgccgcccgc
      421 ccgcgtcgaa ggcggcgcgg catttgtgtc gattatggaa ggctgttcca aatactgctc
      481 cttctgcgtc gtcccctaca cgcgcggcga agaattctcc cgcccgctca acgacgtatt
      541 gaccgaaatc gccaaccttg cccagcaagg cgtgaaagaa atcaacctct tgggacaaaa
      601 cgtcaatgcc tatcgcggcg aaatggacga cggcgaaatc tgcgacttcg ccaccctgct
      661 gcgtatcgtc cacgaaatcc ccggcatcga acgtatgcgc ttcaccacca gccacccgcg
      721 cgagtttacc gactcgatta tcgagtgcta ccgcgacctg cccaaactgg tttcccacct
      781 gcacctgccg attcaaagcg gttccgaccg cgtattgagc gcgatgaaac gcggctacac
      841 cgctttggaa tacaaatcca tcatccgcaa actgcgcgcc atccgtcctg atttgtgcct
      901 gagcagcgac ttcatcgtcg gtttccccgg cgagaccgaa cgcgagttcg agcaaacctt
      961 gaaactggtg aaagacattg ccttcgactt gagcttcgtg tttatttaca gcccgcgccc
     1021 cggcacgcct gccgccaacc tgccggacga cacgccgcac gaagaaaaag tgcgccgcct
     1081 cgaagccttg aacgaagtca tcgaagccga aaccgcccgc atcaaccaaa ccatgatcgg
     1141 cacggttcaa cgctgcctgg tcgaaggcat ctccaaaaaa gaccccgacc agctccaagc
     1201 ccgtaccgcc aacaaccgcg tcgtcaactt taccggcacg cccgacatga tcaaccaaat
     1261 gatcgatttg gaaatcaccg aagcctacac cttttccctg cgcggcaaaa ttgtcgaagc
     1321 ctaaaaccct cacgccgaaa aatgccgcct gaagcgtttc agacggcatt ttgccttgta
     1381 tcggcagacg gcaccgccgc cgttatacgg acaagcggaa taaatttcca aacgcgcaac
     1441 ggcacaccaa gcaccgcccg aaagccgccg cccgaaccct tcctgccgcc tgcggggttt
     1501 caataaaaaa accgcagaaa caaacgccgt ttaaaaacgc cgcccgataa cgacggcgcg
     1561 gtcaagcggt ttaatttgcc gcatcacggt ccggcagcca ctcgcgcacc cggcgttcca
     1621 ccgcttcggc actcaaaccc aaatcgtcca aaagtttttt cggatcgccg tgttcggtta
     1681 cggtatcggc aacgcccaaa agcaaaacgg gtttgcagat gccgtgtttc gccaacactt
     1741 ccaagaccgc gccgcctgcg ccgccctgtt cggcgttttc ttcaagggta acgatgcggt
     1801 cgtggcttcg ggcaaggcgg acaatcaact cttcgtctat cggtttgacg aagcgcatat
     1861 cggcgacggt ggcgttcagt ttttcggcaa ccgccaatgc gggggcgacc atactgccga
     1921 aggcaatgaa ggcggttttc tcaccttcgc ggcggataat gcccttgccg atttccacgg
     1981 tttccatgcc gtctgaaacc ggcgcgcccg tacccgtgcc gcgcggatag cggacggcgg
     2041 cgggcgcatc cgcctgatag caggtcgaaa gcagcaggcg gcattcgttt tcatcgctcg
     2101 gcgcggcaac aatcatgttc ggcacacagc gcaagaagct caaatcgtac aagccggcat
     2161 gggtcgggcc gtccgcgccg acgatgcccg cacggtcgac
//
LOCUS       DAXDSD010000163         2119 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXDSD010000163 DAXDSD010000000
VERSION     DAXDSD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.163"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            203..487
                     /locus_tag="ACFB2Q_002069"
     CDS             203..487
                     /locus_tag="ACFB2Q_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFC0919021.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            488..1675
                     /locus_tag="ACFB2Q_002070"
     CDS             488..1675
                     /locus_tag="ACFB2Q_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC0919022.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
     gene            1890..>2119
                     /locus_tag="ACFB2Q_002071"
     CDS             1890..>2119
                     /locus_tag="ACFB2Q_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC0919023.1"
                     /translation="MNIIGPDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYV"
BASE COUNT          696 a          446 c          522 g          455 t
ORIGIN      
        1 taagcaactt tagccaaagg caggcgaagc atcgcacttt gcgggcgaag ccgcaaacag
       61 ccgagaagcg cgggggggga ttggcgataa gcgcgagggg gtgtccccac agcgctgccg
      121 cgccgcgaat gcggagcaat ctttcagatt aagaaacatt tgtttaatga ggcaaacgtg
      181 ccttttaaga aagggagagc aaatgaaatt gttggccgca ttgattccgc tcttgatgag
      241 cgtcgcaggc cgtatattga ctgcattagg cttgatggct gttacctatg cgggtgtaga
      301 tagattggca gcccattttc aacaggcgat aacccatagc ataacgggcg cgcctcaagc
      361 aatgttacag cttttctata taagcggcgg tggtactgtt ctaaacattc ttttcggcgc
      421 aatcgccttt attctgtcct ttaaacaaat gacaaaactc gcaacctcaa tcgggaagaa
      481 aaaataaatg gcagaaatct gtttgataac cggcacgccc ggttcaggga aaacattaaa
      541 aatggtttcc atgatggcaa acgatgaaat gtttaagcca gatgaaaacg gcgtacgccg
      601 taaagtattt acgaacatca aaggtttgaa gataccgcac acccacatag aaacagacgc
      661 aaagaagctg ccgaaatcaa ccgatgaaca gctttcggcg catgatatgt atgaatggat
      721 caagaagcct gaaaacgtcg gcgcaatcgt tattgtcgat gaggcgcaag acgtatggcc
      781 cgcacgctcc gcaggttcga aaatccccga aaacgtccaa tggctgaaca cacacaggca
      841 tcagggcata gatatatttg tattgacaca aggtcctaaa ctcttagatc agaacttgcg
      901 aacattggtt aaaagacatt accacattgc ggccaacaaa atgggtttgc gtaccctgct
      961 tgaatggaaa gtatgcgcgg atgacccggt aaaaatggca tcaagtgcat tttccagtat
     1021 ctacacactg gataaaaaag tttatgactt gtacgaatcc gcagaaattc acacggtaaa
     1081 caaagtcaag cgttcaaaat ggttttatgc attgcccgtc atcatattat tgattccgct
     1141 atttgtcggt ttgtcttaca aaatgttggg cagttacgga aaaaaacagg aagaacccgc
     1201 agcacaagaa tcggcggcaa cagaacagca ggcagtactt ccggataaaa cagaaggaga
     1261 atcggtgaat aacggaaacc ttacggcaga tatgtttgtt ccgacattgc ccgaaaaacc
     1321 cgaaagcaag ccgatttata acggtgtaag gcaggtaagg acctttgaat atatagcagg
     1381 ctgtatagaa ggcggaagaa ccggatgcac ctgctattcg catcaaggga cggcattgaa
     1441 agaagtgacg gagttgatgt gcaaggacta tgtaaaaaac ggcttgccgt ttaacccata
     1501 caaagaagaa agccaagggc aggaagttca gcaaagcgcg cagcaacatt cggacagggc
     1561 gcaagttgcc accttgggcg gaaaaccgca gcagaaccta atgtacgaca attgggaaga
     1621 acgcgggaaa ccgtttgaag gaatcggcgg gggcgtggtc ggatcggcaa actgaagaaa
     1681 acggcaacag agaaaaaaga cccgtaaacc gtttgaatat agacggctta cgggtctttg
     1741 tttcgcgcaa agcaagggct aaggcagtca ggcagcaaat cccgcaatgt attaaaacag
     1801 acgcgtagaa atgccggctg cctttatcca tcctcaaaat tgaatatcat cctagccgta
     1861 tcaaggctgt ataaataagg aaaataccaa tgaatataat cgggccggac atctcaaagg
     1921 acaccataga cgcaacattg cataaaacaa acggaagtat ccattacatt aaatttaaga
     1981 ataatgatga tggattaaaa cagtttagat tgtggataaa gggaaacaga atcagaaaag
     2041 tctatatcgg catggaggca acaggcatct attacgaaaa ggcagcagat atgctttctt
     2101 cctactatac cgtttacgt
//
LOCUS       DAXDSD010000164         1930 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXDSD010000164 DAXDSD010000000
VERSION     DAXDSD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.164"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(54..433)
                     /locus_tag="ACFB2Q_002072"
                     /pseudo
     CDS             complement(54..433)
                     /locus_tag="ACFB2Q_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(487..1620)
                     /gene="carA"
                     /locus_tag="ACFB2Q_002073"
     CDS             complement(487..1620)
                     /gene="carA"
                     /locus_tag="ACFB2Q_002073"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC0919024.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSSFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
BASE COUNT          401 a          477 c          575 g          477 t
ORIGIN      
        1 aaggaaaaca gcttgtgcag gctgtttttt attttgcata atccgtctga aaattaataa
       61 ctgctgattt ccagcaggtt gccatcggga tcgcgcaggt aaaccgattg gattttgccc
      121 attgcgcctg tgcgcgctac gatgccgctt aaaggtttga tgccgtgtgc ggataattcc
      181 tgtaaaaccg tttccagtgg ggcgtatcgg tcagcaggca taaatccgct gtgccgcagg
      241 cggcgtgttg cgcgttaggc tgaatttccg caccgcgccc gtgtaggttg attttctgac
      301 tgtcaaacaa caaagcttta cggttgttgc caaatgaaac ttcttccatg cccaaaactt
      361 gtgtgtaaaa cgcgatggtt cggtcaatgt cggcaacagt cagtactaga tggtcgagtg
      421 cgctaatttt catttccagc tttctttttc agacggcagc agcatactgt tgccgtctga
      481 aaatcattat gcttgttttg cagccttcat attgccaatg aatttgtcga acaaatagcc
      541 gacatcttgc ggacccgggc tggcttcggg gtgtccttgg aaacagaaca cgggtttgtc
      601 ggtcagctcg atgccttgca aggtattgtc gaacaaggat ttgtgggtaa tgcgtgcgtt
      661 ggcgggcagg gtgtcggcat cgacggcgaa accgtggttt tggctggtaa tgacgacttt
      721 gccgctgtcc aaatcttgca caggatggtt cgcaccgtgg tggctgaagc gcattttcag
      781 ggttttcgcg ccgatggcga ggctgatgag ctggtgtccc aagcagatgc cgaaaatcgg
      841 tttgccgctt tccatcagtt tttgcacggc ttcgatggcg taggtgcaag gctcggggtc
      901 gccggggccg ttggacagga acacgccgtc gggattgagt gccaacacgt cttccgcgct
      961 cgtttgtgcg gggacgacgg tcaggcggca gccgcgcgag gcgagcatac gcaggatgtt
     1021 ggttttcacg ccgaaatcgt aggcgacgac gtggtaaggc tgtttgtcag gggtaacgaa
     1081 acctttgccc aattcccatt cgccttccgt ccattcgtaa gtttccgtgc aggaaacttc
     1141 ttttgccaaa tctttgccga ccatgctgcc gaatgcggcg atgagttctt gcgctttttc
     1201 aacggtggca tccgcacctg tcagaatcgc gccgccttgc gcgccttttt cgcgcaacag
     1261 catggtcagg cggcgggtgt cgatgtcggc gatggcgacg gtttcgttgc gtaccaaata
     1321 gtcgtgcagg ctttcggagg cgcggaagct gctgtgcaag agcggcaggt cgcggataat
     1381 caggcctgcg gcgtaaacgc tgcggctttc ttcatcttcg gcgttggtgc cggtgttgcc
     1441 gatgtggggg taggtgaggg taacgatttg tttgcagtag gacgggtcgg tcagaatttc
     1501 ctgatagccg gtcatcgaag tattgaacac gacttcgccg gaagccgaac cttcgtaacc
     1561 gattgatgtg ccgtggaata cgctgccgtc agcgaggacg aggagggcgg gggtgctcat
     1621 gatgggaatc ctgtttttaa taaaattctt gacaatgccg tctgaaggga cttcggcagg
     1681 gggcatccgg tcggaaaaat ccggtcaaaa aaaaacacgc cgcgcaagat agacgcgtaa
     1741 cgtgcttttc gggcaagtgc ctataccctg aaagcagcgt attctaagac gaaacctaca
     1801 cgatttcaag cgcaaacgcg gcaggcgggc tgatatgttc gcgcgaaaac ggaaatccgg
     1861 ggcataaaac cgcccgctcc gcgcccccta tgcccgttac gcatttcccc acgtccgccc
     1921 ggcgaaacta
//
LOCUS       DAXDSD010000165         1921 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXDSD010000165 DAXDSD010000000
VERSION     DAXDSD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.165"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(142..543)
                     /locus_tag="ACFB2Q_002074"
     CDS             complement(142..543)
                     /locus_tag="ACFB2Q_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919025.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKTENNSDGNPTANYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(947..1492)
                     /locus_tag="ACFB2Q_002075"
     CDS             complement(947..1492)
                     /locus_tag="ACFB2Q_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC0919026.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMKRKPFAW"
     gene            complement(1479..1631)
                     /locus_tag="ACFB2Q_002076"
     CDS             complement(1479..1631)
                     /locus_tag="ACFB2Q_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919027.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            complement(1613..1882)
                     /locus_tag="ACFB2Q_002077"
     CDS             complement(1613..1882)
                     /locus_tag="ACFB2Q_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919028.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPMGILPKPVALSIVQYEND
                     PGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
BASE COUNT          576 a          395 c          318 g          632 t
ORIGIN      
        1 agccaacaac ttcggcaaaa tcaagccggc aagcccctgc atcctgtgcc aatcggagaa
       61 aatgccgtct gaaagccgaa taccgcttca gacggcattc tgattgttgg gtttttaatc
      121 agagttttgt ttgataattt gttattttga ataaaataca ggttctgtcg agctgataac
      181 ttctattaca gaatcgctaa aaaagaaaat ataagcctta taatatttct tttcaaaaaa
      241 accaaaactt tcttctttca accagtccat ataactgtca tctattatct cgaaaaaata
      301 atttgcagta ggattcccat ccgaattatt ttctgtttta ttttcgaata aatattctga
      361 aaattttact ttataagatt catccgttat ttgaaaatga attatattgt caataaatat
      421 ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt gttcggtaac
      481 acaaattttg tccacataaa atctgtgatt attgttagaa aattgtaatg gtttaatctt
      541 cacaatcgtt ttccatatct aagttttgtt ttcttattgc cctgccggat aaagcaggtc
      601 tgcctccgta ctgtctatcc tcacatttac tttcctgcca tcggctttta tctccccggt
      661 tttaccgttt ttgccaactt cgcggctttg gcggtggcaa tgttgaaggc ggcttcgacg
      721 gtttcggcgg cattggggtt gacccgtact catccgcaaa cggcaacacg acggacggtg
      781 tttgcttttc agccgtctgc cgttttgacc aaggtgctgc cgtggaagga gcaggtgtag
      841 gatttttgaa gaactcagtt ctcaatatcc tgcttcaaat gaaggaaaga ccatctgaaa
      901 aacaagctta atttcagacg acctttaatc aaatttaaga aagcccttac caagcaaacg
      961 gtttccgctt catatccgaa aggttgtcaa cttcattatc cagcaagaac tgctcaaaag
     1021 cattccaacc tttcttttcc accaattctg cttcctgttt atacaagggg acaagcaaag
     1081 ggaaaacgat attgtagtgt tcgccataac agacctgaaa atcatcatcg aaataaaatg
     1141 gggcggaaac atacagtgca tccattttgc taccttcgat aattaccctg ggtaattgaa
     1201 taatttcacc tcttaaaata ggttgtctat tgtcaatcaa atattcagcc actcctgata
     1261 gaaaagctgc aatattttct ttattccatt cttcagaaca ggtaaaaatt aattcaaacc
     1321 tactcttata gttcaagtca tatcgattca agcctaaagt agaaaaagta gttacccctt
     1381 tatatggttg tgatgggaag acggaaacgc tgagatgata acgtcgatcc aagcatttta
     1441 taccgctttc gatttcaccc aaataatgtt ctaaatgttc aactataatc attttttctt
     1501 aaagatttca tccctatccc ggcaagacgt aggctgtttt gggctccaat accgactttt
     1561 ttattgacgg aaatgccatc tgaaaaataa agcggcatct tgattttgcc gtctaaggac
     1621 tttgcatcca ttcctctttg ctgattccaa attcaaattc agcctgttca aatgccgaat
     1681 ctaatgtgtc atgataagta tccgtttgtt cctgaccggt ctcatccaaa taaaacaaat
     1741 aaaatcccgg gtcattttca tattgaacaa tacttaaagc aacaggtttg ggaagtatcc
     1801 ccattggagt gcgatgaatc gttctaatag gaaaatcttt atttttatct aagataactt
     1861 tgaataattc ttttacactc ataattttct aatcctgaat aattgttact acactatttt
     1921 c
//
LOCUS       DAXDSD010000166         1911 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXDSD010000166 DAXDSD010000000
VERSION     DAXDSD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.166"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1525
                     /locus_tag="ACFB2Q_002078"
     rRNA            <1..1525
                     /locus_tag="ACFB2Q_002078"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1622..1735
                     /gene="rrf"
                     /locus_tag="ACFB2Q_002079"
     rRNA            1622..1735
                     /gene="rrf"
                     /locus_tag="ACFB2Q_002079"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1771..1890
                     /locus_tag="ACFB2Q_002080"
                     /pseudo
     CDS             1771..1890
                     /locus_tag="ACFB2Q_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
BASE COUNT          484 a          422 c          567 g          438 t
ORIGIN      
        1 cgtagtcgat gggaaacagg ttaatattcc tgtacttgat tcaaatgcga tgtggggacg
       61 gagaaggtta ggttggcaag ctgttggaat agcttgttta agccggtagg tggaagactt
      121 aggcaaatcc gggttttctt aacaccgaga agtgatgacg agtgtctacg gacacgaagc
      181 aaccgatacc acgcttccag gaaaagccac taagcttcag tttgaatcga accgtaccgc
      241 aaaccgacac aggtgggcag gatgagaatt ctaaggcgct tgagagaact cgggagaagg
      301 aactcggcaa attgataccg taacttcggg agaaggtatg ccctctaagg ttaaggactt
      361 gctccgtaag ccccggaggg tcgcagagaa taggtggctg cgactgttta ttaaaaacac
      421 agcactctgc caacacgaaa gtggacgtat agggtgtgac gcctgcccgg tgccggaagg
      481 ttaattgaag atgtgcaagc atcggatcga agccccggta aacggcggcc gtaactataa
      541 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acccgcacga atggcgtaac
      601 gatggccaca ctgtctcctc ccgagactca gcgaagttga agtggttgtg aagatgcaat
      661 ctacccgctg ctagacggaa agaccccgtg aacctttact gtagctttgc attggacttt
      721 gaagtcactt gtgtaggata ggtgggaggc ttggaagcag agacgccagt ctctgtggag
      781 tcgtccttga aataccaccc tggtgtcttt gaggttctaa cccagacccg tcatccgggt
      841 cggggaccgt gcatggtagg cagtttgact ggggcggtct cctcccaaag cgtaacggag
      901 gagttcgaag gttacctagg tccggtcgga aatcggactg atagtgcaat ggcaaaaggt
      961 agcttaactg cgagaccgac aagtcgggca ggtgcgaaag caggacatag tgatccggtg
     1021 gttctgtatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     1081 ttccgcccaa gagttcatat cgacggcgga gtttggcacc tcgatgtcgg ctcatcacat
     1141 cctggggctg tagtcggtcc caagggtatg gctgttcgcc atttaaagtg gtacgtgagc
     1201 tgggtttaaa acgtcgtgag acagtttggt ccctatctgc agtgggcgtt ggaagtttga
     1261 cgggggctgc tcctagtacg agaggaccgg agtggacgaa cctctggtgt accggttgta
     1321 acgccagttg catagccggg tagctaagtt cggaagagat aagcgctgaa agcatctaag
     1381 cgcgaaactc gcctgaagat gagacttccc ttgcggttta accgcactaa agggtcgttc
     1441 gagaccagga cgttgatagg tggggtgtgg aagcgcggta acgcgtgaag ctaacccata
     1501 ctaattgccc gtgaggcttg actctatcat ttgaaggact tcaagagata aaagcttact
     1561 gactgattca gtcattaccg aatatattga ttaaggcttt accgatttgc aacagtttaa
     1621 gtttggcggc catagcgagt tggtcccacg ccttcccatc ccgaacagga ccgtgaaacg
     1681 actcagcgcc gatgatagtg tggttcttcc atgcgaaagt aggtcactgc caaacaccct
     1741 ttcagaaaac ccccggatat ccgggggttt ttgctttgcc cggaaaaaat gtcggggatg
     1801 gcgggacggc atctgtacgg tgtccggtcg ggtttgcgga ggaacggctt gaaactttgg
     1861 gatattcatt ttagaatgac ccgttttata gcggattaac aaaaatcagg a
//
LOCUS       DAXDSD010000167         1887 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXDSD010000167 DAXDSD010000000
VERSION     DAXDSD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.167"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(214..1494)
                     /gene="hemL"
                     /locus_tag="ACFB2Q_002081"
     CDS             complement(214..1494)
                     /gene="hemL"
                     /locus_tag="ACFB2Q_002081"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC0919029.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1558..1887)
                     /locus_tag="ACFB2Q_002082"
                     /pseudo
     CDS             complement(1558..1887)
                     /locus_tag="ACFB2Q_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
BASE COUNT          409 a          531 c          527 g          420 t
ORIGIN      
        1 cagacggcat ttttacagta gattgaaatc aaaaatgtac acgcccgtca ttcccgcgca
       61 ggcgggaatc cggaaggtcg ggcttgccgt tattttcaat cattacagaa actgaaaggt
      121 ccggattccc gcctgcgcgg aaatgacggc ggtgtgcatt cttacttcaa tctgccaaac
      181 aaatcgaaca gagaaactct gtccgtcaaa acatcatgca gccatcgcct tgaacacttc
      241 aaccgcaacc gcaaccgttt cgtcaatcag ctcgggcgta tgcgcggcgg aaacgaaacc
      301 tgcttcataa gcggacgggc cgaaggcaat gccgcggtcg agcatgccgt ggaagaagcg
      361 tttgaaagcg tcgatattgg agcgcgccat atcggcatag tttcgcggca cgtgtgcggc
      421 gaaatacaga ccgaacatac cgcccacgct gtcggcggca aactcgatac cgtgcgcttt
      481 ggcggcggca ataccgttgg caaggcgttg tgtcaaggcg gtcaggtttt catagaagcc
      541 ttcgcgctgg atgatttcca gcgttttcaa gccggcggcg acggcaatcg ggttgcctga
      601 taatgtacct gcctgataca cgccgcccaa cggggaaata cattccatga tgtctttgcg
      661 tccgccgaac gcggcaagcg gcataccgcc gccgatgact ttgcccatcg tggtcaggtc
      721 gggcgtgatg ccgtgcagcg actgcgcgcc gccgagcgcg acgcggaaac cggtcatcac
      781 ttcgtcgtaa atcaacaccg cgccgtgttt ttcggtcaat ccgcgcaagg ctttgacaaa
      841 ggcttcggtc gggcggacga ggttcatatt gccgacgaag ggttcgacaa tcacgcaggc
      901 gatttcgtcg ccgctttggg caaaggcttc ttcgagttgg gcgatgttgt tgtattcgag
      961 taccaaagta tgtttggtaa agtcggcagg cacaccggcg gaagaaggat tgccgaaggt
     1021 aagcagaccg ctgcctgctt tcaccaacag gctgtcggaa tggccgtggt agcagccttc
     1081 aaatttgatg attttgtcgc ggccggtaaa accgcgtgcc agacggatgg cagtcatcgt
     1141 cgcttccgtg ccggagctga cgaggcgcag ccgttcgaca gacggcataa tttcggcaat
     1201 ttgttcggca atggcgattt cgccttcggt gggcgcgccg aacgacaaac cgcccaacgc
     1261 agcttcgcgc acggcttcga cgacttcggg atgcgcgtgt ccgacaatcg caggccccca
     1321 agagccgaca taatcggtgt agcgcgtgcc gttttcgtcc caaacatacg cgccttcggc
     1381 ttttttgatg aagcgcggca cgccgccgac gctgccgaat gcgcgcacgg gcgaattcac
     1441 gccgccgggg atgatggctt tggcgcggtc gaataaaatt tcgttacggt tcatatttat
     1501 cctcaaatgc cgtctgaacg gcaggtttcg ggcttggaag cagaaagccc cattttatca
     1561 tttttcaggt tgcgacaagg atttgcccgc ttctttgcgg attacgccaa ccgtatcccg
     1621 gatgacggaa cgctcgtctt tttcctcttt atatgtgtaa agcggtagtc ccggacgact
     1681 ccctccccgt cgtaatccac gcaccattcc caatgccgcc gctccgattt catatagatg
     1741 aaattggtcg gcaaaaaatt ataaatcggc aggctgacct cgtgataggc ataacagccg
     1801 aaagggttgc gcttcccgaa acgtgcctct acctccgccc gggtcgtttt gcctttaaca
     1861 accgtttgtg cgattccctc ttccgtc
//
LOCUS       DAXDSD010000168         1863 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXDSD010000168 DAXDSD010000000
VERSION     DAXDSD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.168"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(38..763)
                     /gene="aat"
                     /locus_tag="ACFB2Q_002083"
     CDS             complement(38..763)
                     /gene="aat"
                     /locus_tag="ACFB2Q_002083"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC0919030.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACFB2Q_002084"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACFB2Q_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC0919031.1"
                     /translation="MEKFSNIAQLKDSGLKVTGPRLKILDLFEKHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1494..>1863
                     /locus_tag="ACFB2Q_002085"
     CDS             1494..>1863
                     /locus_tag="ACFB2Q_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC0919032.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNAD"
BASE COUNT          469 a          616 c          410 g          368 t
ORIGIN      
        1 ccactatatt tgaacctgac cgaagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatccgcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgta tcctgctggc
      181 agtctatcag ttccacgccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgcaac gcaaacatcg attcgccata aaacaccctg ccgatctgaa
      301 cgccgtaaaa gccgcccgcc aacctcgttt taccgttttc atcgggataa tggcactcga
      361 aagaatgcgc gtgtcccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagtccc atcctgattc gggcgcgccg ctgccgcaca atgcgcgaca acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttacgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaattaccg cacgcggccc gaccgcatac caaaaaaacc
      601 aaccgtcccg cgaaaaccaa ggaaacacgc cgttccgata cgcctcgagc agccgccccg
      661 cgtccaaatc cctgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcctcggcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcgtg gtggtcgccc ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatggt ggcgttgcag aatgcccgcc tgctcgaact
     1081 gcgtcagcac gcggtaaatc gtcgccacac cgatttccac gccctcttcc agcagaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcgtgctt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgcgcaatg ttgctgaatt
     1261 tttccataat attcaatatc cctgtaaaat aatagacgct ataatacgca atttcagcct
     1321 gcttgcccac tatcacacca tagcagttcg caatagcaaa accgcaacgg cggacgggca
     1381 gtcggacaca gacagggtaa aataccgctt aagcgtatga ttatcgttcg cttttataaa
     1441 atattcaagc agtgctacac tagacatccc gatttgcaca gaaaggttct cccgtgaaca
     1501 aaaccctcat cctcgccctt tccgccctgt tcagcctgac cgcgtgcagc gtcgaacgcg
     1561 tctcgctgtt tccctcctac aaactcaaaa tcatccaagg caacgaactc gaaccgcgcg
     1621 ccgttgccgc cctgcgcccc ggcatgacca aagaccaagt cctgctcctg ctcggcagcc
     1681 ccatactgcg cgacgctttc cataccgacc gctgggacta taccttcaac acctcccgca
     1741 acggcatcat caaagaacgc agcaacctga ccgtctattt tgaaaacggc gtactcgtcc
     1801 gcaccgaagg cgacgccctc caaaatgccg ccgaagccct ccgcgcgaaa caaaacgcag
     1861 aca
//
LOCUS       DAXDSD010000169         1806 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXDSD010000169 DAXDSD010000000
VERSION     DAXDSD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.169"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..342
                     /gene="rpsD"
                     /locus_tag="ACFB2Q_002086"
     CDS             <1..342
                     /gene="rpsD"
                     /locus_tag="ACFB2Q_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC0919033.1"
                     /translation="LESRLDNVVYRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQV
                     KAGDVVSVREKAKKQVRIQEALGLATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDI
                     NEQLVVEFYSK"
     gene            368..1354
                     /gene="rpoA"
                     /locus_tag="ACFB2Q_002087"
     CDS             368..1354
                     /gene="rpoA"
                     /locus_tag="ACFB2Q_002087"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC0919034.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            1378..1746
                     /gene="rplQ"
                     /locus_tag="ACFB2Q_002088"
     CDS             1378..1746
                     /gene="rplQ"
                     /locus_tag="ACFB2Q_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC0919035.1"
                     /translation="MRYRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
BASE COUNT          473 a          319 c          452 g          562 t
ORIGIN      
        1 ctggaatctc gtttggataa tgtcgtttat cgtatgggtt tcggttctac ccgagctgaa
       61 gcaagacagc ttgtttctca taaggctata gttgtaaatg gacaagttgt caatattcct
      121 tctttccaag tgaaagctgg tgatgttgtc tcagttcgtg aaaaagccaa aaaacaggta
      181 cgtattcaag aagcattggg tttggcaact caaatcggct tgccgggttg ggtttctgta
      241 gatgcagata aacttgaggg tgtgttcaaa aacatgccgg atcgctctga attgaccggt
      301 gatattaatg aacagctggt ggtagagttc tactctaaat aatgctagct cagtgaggga
      361 cagttaaatg cagaatagca caaccgaatt tttgaaacct cgtcaaattg atgtaaatac
      421 tttttctgca actcgtgcaa aagtatctat gcagccattt gaacgtggtt tcggtcatac
      481 cttaggtaat gctttgcgcc gtatcttact gtcatccatg aatggttttg ctcctactga
      541 agtagctatt gccggtgtat tgcacgaata ttctactgtt ggtggtgttc aggaagatgt
      601 tgttgacatt ttgctgaata ttaaaggtat tgtgtttaaa ctccatggtc gtagccaagt
      661 tcaacttgtg ttgaagaaat caggttcagg tgtcgtatct gccggtgata ttgagttgcc
      721 gcatgatgta gaaattctga atcctggtca tgtcatttgt catttggctg ataacggtca
      781 aattgaaatg gaaattaaag tagagcaagg tcgtggttat caatctgttt caggtcgtca
      841 ggtagttcgt gatgagaacc gtcagattgg tgcaatccag ttggatgcga gcttttcgcc
      901 catcagccgt gttagctttg aggttgaacc tgcacgtgta gagcagcgga cggatcttga
      961 taaattggtt ttggatatcg aaaccgacgg ttctattgat cctgaggaag ctgtacgcag
     1021 tgcggcacgt attttgattg atcagatgtc tatttttgct gatttgcagg gtactcctgt
     1081 ggaggaggtt gaagaaaaat cacctcctat cgaccctgtt cttttgcatc cggtggatga
     1141 tctggaattg acagtacgtt cagctaattg tttgaaagct gaggatattt attatattgg
     1201 cgatttgatt caacgcactg aaaccgagct tcttaaaacg ccgaatttgg gacgtaaatc
     1261 tttgaatgag attaaggaag tattggcatc taaaggtttg acactgggtt ctaagttgga
     1321 agcatggcca cctgtaggct tggaaaagcc ttaatgaaga attaaaggat aattgatatg
     1381 cgttatcgta atggcaatcg caaattaaac cgtaccagca gtcatcgtgc tgcaatgctg
     1441 cgtaatatgg cgaattcatt attgactcac gaaactattg taacaactct gcctaaggcc
     1501 aaggaattgc gccgtgtagt agagccgttg attacattgg gtaaaaagcc gtcattggca
     1561 agccgccgtt tggcatttga ccgtactcgc gaccgtgatg ttgtagtaaa actgtttggc
     1621 gatttgggtc ctcgttttac tgctcgtaac ggtggttatg ttcgagtgtt gaaatacgga
     1681 ttccgtaaag gtgataatgc acctctggca ctggttgaat tggttgacaa accggctgct
     1741 gagtaatttt agtcatataa cgccatctgc cgaaaagcag gtggcgttat ttttgcaata
     1801 tctgat
//
LOCUS       DAXDSD010000170         1796 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXDSD010000170 DAXDSD010000000
VERSION     DAXDSD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.170"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(140..215)
                     /locus_tag="ACFB2Q_002089"
     tRNA            complement(140..215)
                     /locus_tag="ACFB2Q_002089"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Ala,seq:tgc)
     gene            complement(221..297)
                     /locus_tag="ACFB2Q_002090"
     tRNA            complement(221..297)
                     /locus_tag="ACFB2Q_002090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(261..263),aa:Ile,seq:gat)
     gene            complement(398..>1796)
                     /locus_tag="ACFB2Q_002091"
     rRNA            complement(398..>1796)
                     /locus_tag="ACFB2Q_002091"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          383 a          547 c          403 g          463 t
ORIGIN      
        1 aaaaaatcaa aacaaactca ttgtctttgt ttgttgattt cggctttcca atttgttaaa
       61 gatcgatgcg tcgttattct acttcgcaaa tcaaaataag ctgctaaaaa cagcaaactt
      121 gctttcattt gtaaagtttt ggtggaggca aacgggatcg aaccgatgac cccctgcttg
      181 caaagcaggt gctctaccaa ctgagctatg cccccgttct tggtgggtct gggaggactt
      241 gaacctccga ccccacgctt atcaagcgtg tgctctaacc agctgagcta caaacccggt
      301 ttcccttctt aagcgaacct tgccttcact caagcttctt ccgcatcttt tcagtttacc
      361 gataagtgtg aatgcctaaa gccccttctt tctctagaaa ggaggtgatc cagccgcagg
      421 ttcccctacg gctaccttgt tacgacttca ccccagtcat gaagcatacc gtggtaagcg
      481 gactccttgc ggttacccta cctacttctg gtatccccca ctcccatggt gtgacgggcg
      541 gtgtgtacaa gacccgggaa cgtattcacc gcagtatgct gacctgcgat tactagcgat
      601 tccgacttca tgcactcgag ttgcagagtg caatccggac tacgatcggt tttgtgagat
      661 tggctccgcc tcgcggcttg gctaccctct gtaccgacca ttgtatgacg tgtgaagccc
      721 tggtcataag ggccatgagg acttgacgtc atccccacct tcctccggct tgtcaccggc
      781 agtctcatta gagtgcccaa ccgaatgatg gcaactaatg acaagggttg cgctcgttgc
      841 gggacttaac ccaacatctc acgacacgag ctgacgacag ccatgcagca cctgtgttac
      901 ggctcccgaa ggcactcctc cgtctccgga ggattccgca catgtcaaaa ccaggtaagg
      961 ttcttcgcgt tgcatcgaat taatccacat catccaccgc ttgtgcgggt ccccgtcaat
     1021 tcctttgagt tttaatcttg cgaccgtact ccccaggcgg tcaatttcac gcgttagcta
     1081 cgctaccaag caatcaagtt gcccaacagc taattgacat cgtttagggc gtggactacc
     1141 agggtatcta atcctgtttg ctacccacgc tttcggacat gaacgtcagt gttatcccag
     1201 gaggctgcct tcgccatcgg tattcctcca catctctacg catttcactg ctacacgtgg
     1261 aattccacct ccctctgaca cactcgagtc acccagttca gaacgcagtt cccgggttga
     1321 gcccggggat ttcacatcct gcttaagtaa ccgtctgcgc ccgctttacg cccagtaatt
     1381 ccgattaacg ctcgcaccct acgtattacc gcggctgctg gcacgtagtt agccggtgct
     1441 tattcttcag gtaccgtcat cggccgccga tattggcaac ggccttttct tccctgacaa
     1501 aagtccttta caacccgaag gccttcttca gacacgcggc atggctggat caggcttgcg
     1561 cccattgtcc aaaattcccc actgctgcct cccgtaggag tctgggccgt gtctcagtcc
     1621 cagtgtggcg gatcatcctc tcagacccgc tactgatcgt cgccttggtg ggcctttacc
     1681 ccgccaacca gctaatcaga tatcggccgc tcggatagcg caaggcccga aggtcccctg
     1741 ctttccctct caagacgtat gcggtattag ctgatctttc gatcagttat cccccg
//
LOCUS       DAXDSD010000171         1779 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXDSD010000171 DAXDSD010000000
VERSION     DAXDSD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.171"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            149..1651
                     /locus_tag="ACFB2Q_002092"
     CDS             149..1651
                     /locus_tag="ACFB2Q_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFC0919036.1"
                     /translation="MNIKEFMSNYTNHPVLFIGTGMSLRYLDNSYTWDGLLSKIAIDL
                     FGDDREYLNIKSRYCEDGRFQYEEIAEELQSKFDKVLENDPDGRFKEINDKFFENMRA
                     GNTLSRFKIYISTLLSQLNYKDNSNTELSELKKARKNVGSIITTNYDKLAQDIFEFNP
                     LIGNDILLSNPYGSVYKIHGCVDDPSKIIITKKDYEKFKEKYELIRAQLLSLFIHNPI
                     IFLGYNVGDENIKEILKTIFTYVEPNSPSANKIRRNFLLVEYEPESNNEDIVEHDIDI
                     TGFSTIRINKIKTDNFSQIYKALAELTLPISAMDVRKFQSIAKEIYTGGNIKVSFTED
                     MDNLNNSDKVVAIGSTKTISYNFQTTSEMMSNYFKIIEEENSQLLKLIDKHSIASTQY
                     FPIYGFSRICSDIHKEAVLKRQQKEKLDHFIEEINRRCKNNHSSIQSILDDENISDTY
                     KNDAIAWGIWNNQLSEDEVENYLKNFVNKKNTHYKRLLCMFDYKKYADTV"
BASE COUNT          694 a          261 c          289 g          535 t
ORIGIN      
        1 tttttagaaa tgcccgactc gcggtatatc aatgacgggt ttcaggtgtt gttggaattg
       61 ggggcagtgg aggttgtctg aaaacaggca gatcgggcgt ttatgcccaa cgagaacctt
      121 attgtcacta ttattaggga ttaatacaat gaatataaaa gaatttatgt ctaactatac
      181 caaccatccc gttctcttta ttggaacagg tatgagtttg agatacttag ataattcata
      241 tacttgggat ggtttattat ctaaaattgc aatagattta tttggagatg atagggaata
      301 tttgaacatc aaatcacggt actgtgaaga tggtagattc caatatgaag agattgcaga
      361 ggaattacaa agtaaatttg ataaagtttt agaaaatgac cctgatggta gatttaaaga
      421 aataaatgat aagttctttg aaaatatgag ggcgggaaac accttgagca ggtttaagat
      481 ttatatatcc accctgctct cacaattgaa ttataaagat aattctaata cagaattatc
      541 tgaattaaaa aaagcgagaa aaaatgtagg gtcaatcatt acaacaaatt atgataaatt
      601 agcccaagat atttttgaat ttaacccact aattggtaat gatattcttt taagcaaccc
      661 ttatggctca gtatacaaaa tacatggttg tgtggacgat ccatcaaaaa ttattattac
      721 caaaaaggat tatgagaaat ttaaagaaaa atatgaactt attagagccc aactattatc
      781 gttatttatt cacaatccaa tcatatttct tggatataat gttggtgacg aaaatatcaa
      841 agaaatttta aaaacaatct ttacttatgt agaaccaaac tctccttcag ctaataaaat
      901 tcgtaggaat tttttactcg tagaatatga acctgagtct aacaatgaag atattgttga
      961 acatgatata gatataactg gattctctac tatccgtatt aataaaatca aaacagataa
     1021 cttctcacaa atttataaag ctcttgcaga actaacacta ccaatctcag ctatggatgt
     1081 acgtaaattt caatctatag caaaggagat ttatactggc ggtaacatta aagttagctt
     1141 tacggaagat atggacaatt taaataatag cgataaagtg gttgctattg gttcaactaa
     1201 aactatcagc tacaactttc aaacaacatc agagatgatg tcaaattatt tcaaaataat
     1261 tgaagaggaa aattcacaac ttctaaaatt aatagataaa catagtatag catctacgca
     1321 atattttcct atttatggat ttagtaggat atgttctgat atacataaag aagctgtact
     1381 aaaacgccaa caaaaagaaa aattagatca ttttattgaa gaaataaata ggcgttgtaa
     1441 aaataatcat tcatccatcc aatcaatttt agatgatgaa aatatttcag acacatataa
     1501 aaacgatgcg attgcttggg gaatatggaa taaccaactt tcagaagatg aggttgaaaa
     1561 ttatttaaaa aattttgtga ataaaaaaaa tacgcactat aaaagactac tatgtatgtt
     1621 cgattataaa aaatatgcgg atactgtcta aactctaaaa aataaggtcg ggcatttatg
     1681 tccgacctac aaaactatgc aaaacactaa aaatcaagca cgcagctcag gcattcatgc
     1741 ccaacacaat ccgacaaatg acaaaccact ttcagacaa
//
LOCUS       DAXDSD010000172         1764 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXDSD010000172 DAXDSD010000000
VERSION     DAXDSD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.172"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(766..>1764)
                     /gene="pilQ"
                     /locus_tag="ACFB2Q_002093"
     CDS             complement(766..>1764)
                     /gene="pilQ"
                     /locus_tag="ACFB2Q_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC0919037.1"
                     /translation="LGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSV
                     LIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGA
                     TGRKKLKNETSAFGWGVNSGFGGGDKWEAQTKINLPVAAAANSISLVRAISSGALNLE
                     LSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLG
                     LTVTPNITPDGQIIMTVKINKDSPAQCASGNNTILCISTKSLNTQAMVENGGTLIVGG
                     IYEENNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
BASE COUNT          402 a          434 c          486 g          442 t
ORIGIN      
        1 agtccgttcg gtttcagccg tatccgacag attcctgccg cgttgcggtt ctagattccc
       61 gcctgtgcgg gaatgacggc atatcggcag gcgaaggata aatgcgtaag gcggatgcgt
      121 aagatgggtt gtagggtgga ctgtagggtg tgcttcagcc cgccgattcc aaccaacttc
      181 gccaaaaacc gaaaccgccg agttacgcct attccccaaa acccttgatg cggtgaaatt
      241 agtgggctga agcccaccct acggcccgat gaatcgtcag taatacccga atcgtcattc
      301 ccgcgcaggc ggaaatccga acacgtccgc acggaaaccc atatcccgtc attcccgcga
      361 aagcgggaat ctagaatttc aatgaggcaa gaatttatcg gaaaaaaacc gaagtttaaa
      421 gacctagatt cccgcctgct cgggaatgac ggggtgtttc gggttgctgt tttttgtgga
      481 aatgacgagg ctttggattg cgaggattta tcccttccgc cgtcattccc acaaaagtgg
      541 gaatctagaa atgaaaagca acaggaattt atcggaaatg accgaaactg aacggactgg
      601 atttccgatt tcgcgggaat gacggctcaa aagttacgag acgaaaaaca accgaaaccg
      661 gacaagttgg attcccgcct gcgcgggaat gacggcattt cggtcgcggc aaaaagcata
      721 aagaaagggc atatgctgta aaacatatgc ccttattttg acgcatcaat agcgcaggct
      781 gttgccggcc gtatctataa tcctcggggt aatgaaaatc agcagttcgc ggcggtcggt
      841 ttttttcccg cgtgttttaa agaggttgcc gataacgggg atgtcgccca acagggggac
      901 tttggtcagc gtattgccgt tgttttcttc ataaataccg ccgacaatca aagtgccgcc
      961 gttttcaacc atagcctgcg tattcaggct tttggtcgaa atacatagga ttgtgttgtt
     1021 gcctgaagca cattgtgcag gcgagtcttt gttgattttg acggtcatga tgatttgtcc
     1081 gtcgggcgtg atgttcggcg taacggtcag ccccaagacg gcttttttga gttccgtgtt
     1141 ggtagagttg ccgccgcccg aggctgtagt tacggtaaaa ggaatttcgt aaccggattc
     1201 gattttggcc tctttgcggt tttgggtcag cacgcgcgga ttggcaagcg ttttggtttt
     1261 tgaaagcgac tcggatgcgg acaattccaa attcaacgcg ccggaggaaa tcgcgcgcac
     1321 cagcgaaatg ctgtttgcgg cagcggcaac cggcaggttg attttggttt gggcctccca
     1381 tttatcgccg cccccgaagc cggagttcac gccccagccg aatgcgctcg tctcattttt
     1441 cagttttttc ctgcctgtcg cgccgaactt aacgcccaaa tcgcgcgaga agccgtctgc
     1501 cgcttcgacg atacgcgcct caatcatcac ttgttgcgcg ggtacgtcca attcgtcaat
     1561 cagtttgcgg aatttttcga tgacgctgcg ggtatcggta acaatcaggg tgttggtggc
     1621 gggatcgatc agcacgctgc ccctgccgct gacaagcgtg ttgcggtttc cggtcgtgtc
     1681 ggcattgtcc aaacgcagga tgctgcggaa ttcttccaca tttttgtatt tcaattggaa
     1741 gttttgggaa tacagcgcgc ccag
//
LOCUS       DAXDSD010000173         1729 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXDSD010000173 DAXDSD010000000
VERSION     DAXDSD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.173"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(1..83)
                     /locus_tag="ACFB2Q_002094"
                     /pseudo
     CDS             complement(1..83)
                     /locus_tag="ACFB2Q_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(101..445)
                     /locus_tag="ACFB2Q_002095"
     CDS             complement(101..445)
                     /locus_tag="ACFB2Q_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919038.1"
                     /translation="MNLENYENILIKLLFHHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDPLPD
                     NMIINNFFEIND"
     gene            complement(844..1245)
                     /locus_tag="ACFB2Q_002096"
     CDS             complement(844..1245)
                     /locus_tag="ACFB2Q_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919039.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTGKTIKISIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDERYAAWISKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIES"
     gene            complement(1249..>1729)
                     /locus_tag="ACFB2Q_002097"
     CDS             complement(1249..>1729)
                     /locus_tag="ACFB2Q_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919040.1"
                     /translation="GYKKPTARHMQTKALGTVDEIGNTVQQVGKQTSGQKTSGGNPAI
                     DSDPYSPSSVSARIEAGKVRSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDF
                     NSLNVRNVQTRPNGTITGNLPDGRAVNVRNDSSGGEPTLEITISNNRKIKIRYGNTR"
BASE COUNT          540 a          339 c          260 g          590 t
ORIGIN      
        1 tgaaaacccg ttaaaaaagg attaaccgca ccttggacga tgccgcgaat atcatccata
       61 cggttacccc accaattttt attggtttat tggtatatcg ttaatcattt atttcaaaaa
      121 agttatttat tatcatatta tcagggagcg ggtcgttaaa aaaaactccc tcaaattcca
      181 ttaaattagt atttatccag cacataaacg aaccaaagcg ttctttccct gagtgtaaat
      241 atttaaaatt aaactcaaaa taagtttttt tgcttagaaa atttaatggt ttttctatag
      301 tgaaaaaaaa gcccttactc gttttagttc gagatactat tttaaataag tttttattct
      361 caataaaata agaatattca tttactaagt tgttatgatg aaaaagtaat tttattaaaa
      421 tgttttcata attttctaga ttcatttcta gctcaattta aaatgatttc attgttgccg
      481 taccaatgcg aaatttacca tcaacaaaat ttcccttcac tgatacaggt accccatcgc
      541 agagatgtag gtcggatatt tgtatccgac aaaaatattt gacgcgttta tcgtttccga
      601 aatgccgctg ttagaaaatg tcggattcga gaatccaacc tacggctata tccgtaaagt
      661 ctgaccattg ttcaatgcgg aaggcgcgaa tattgcggac ggaatgtaga aaccaatata
      721 cttaattgcc atgccattct catcaaaata cctgtgctgt ttaaagtgcg tggttcatta
      781 cacgagtaat tttattttca ggctgcctga aagaaagata ggcaacttat tactcatctt
      841 acgctagctc tcaataattg aatacgttgc actagaacca attatttcta tgaatgtttg
      901 ctcaaacata aaaatatagt atttaaagaa ttctctattc aaaggaaaat aactttcttt
      961 cgatatccat gccgcataac gctcatcaag aagttcgaaa aaagattgac caccattttc
     1021 atgaagcaaa gctctatctg catcttccga aattaaatcc ataaaagtat ctataaaata
     1081 cgattcatca acaaccctaa aatgaataac atcctcaata gaaattttta tggttttacc
     1141 cgtatttcta taaccaactg ctataacttc tactttatca aagtttattg ctgcattatc
     1201 aaaatagaaa tagccagttg ggaaatctaa gctttttaat ttcataattt atcgtgtatt
     1261 tccatatctg atttttattt ttcggttatt actaattgtt atttcaagtg ttggttctcc
     1321 accactgcta tcattacgaa cattaaccgc acgcccatca ggtaaattgc ccgttatcgt
     1381 tccattagga cgtgtttgta catttcgaac attcagacta ttaaaatcat ttagtgcgtc
     1441 attgtaatcc cccactttat catactgaac agcgggacct tttgttttac tcctttgagt
     1501 agtattgctc aaaatgtctt tgatttgtaa atcactgcgc accttaccgg cttctatacg
     1561 agctgacaca ctactcgggc tataggggtc gctatcaatc gcaggattac caccgctggt
     1621 tttttgtccg ctagtctgtt tcccaacctg ctgtactgta ttgccaattt catctaccgt
     1681 acctaacgcc ttagtctgca tatggcgggc agtaggtttt ttataaccg
//
LOCUS       DAXDSD010000174         1708 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXDSD010000174 DAXDSD010000000
VERSION     DAXDSD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.174"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(208..1593)
                     /locus_tag="ACFB2Q_002098"
                     /pseudo
     CDS             complement(208..1593)
                     /locus_tag="ACFB2Q_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
BASE COUNT          485 a          521 c          313 g          389 t
ORIGIN      
        1 ccaccctgct tgtccgatgc ctcgggacgg acgggcaaac cgacacggca cgcaaaccgc
       61 gtcggcaaaa atgccgtctg aaaaaattca gacggcatct tcagacacat tacctgcaaa
      121 cggcaacaca ttccaatcca aaccgatcag gcaatcaata cgaacggctg tttacatact
      181 taccagcttt caccaaccgg tatcgattta accgatttcc ttaatatttt tcctgtccgt
      241 tttaaacttc gccttaaaca catccggtaa atctttatcg aaataccaaa gcccgtcatc
      301 catttccaat gcgccgccca ttccatgcag aacgactttt cccccgttaa aggatcggtt
      361 tcggtaattt ccacctcatg catttttccc caatccaaaa caggcaactt gtgtgcaaac
      421 gccaaaactt gttcgtcaaa acggccgcac gccttatcgc atcggcctga aacatatacg
      481 ggacaaggct catcctctgc ataaccgtcc ggaaggatac cggctgaggc aggacacaat
      541 ccgtatgttt ccgcccacga cgacaaagcc cgtctcgccg cctttttatt ggcaaataat
      601 ccggtaggcg gattatccgt cacaccgttt ttcaaagccg ctgttttcgc attcaacatg
      661 ccgtctgaac ctttttcaaa cctgacggtc gtaaatgttt taagcagatt tttggcagat
      721 acataacaac ccgaatgata acgtccgacc aattccgctt taatcttata tgcatgcagg
      781 ctgcccaatg cgggaaaaaa acggacttcc tccgtattgc accaatcaaa cgggggcttt
      841 tccggaatcc aacaaagccg caacctcgcc atatgcccgt tcaaacgtac cgatataatt
      901 tacttttccc ccgccgtcga aacaggcaag cacacctgta ccgtcgggca aaccgtacag
      961 ccgttccctc aaccgttcgg gaagcgcggc aggaagaagt ttcggattcg tcaaacggaa
     1021 acactgcctg ctccactcct caagcccgtg tgccgacagg ctgtattcca aataatcaca
     1081 caacgccgat acatccgcca tcgcacgatg cctgtcttcc acaacaatcc ccaacctttc
     1141 gatgataccg tccaggctgt gcttgtaaaa ctgcggatac agccgacgcg aaagctgcac
     1201 actgcacaaa gcaggcgatg aaaatccgat acccgcacga tgaaactcat gctttaaaaa
     1261 cgtatagtcg aaacggctgt tgtgcgccac cagcacgcac cccttcaata ccgaaaacaa
     1321 cccgccggca atttcagcaa acaccggcgc gtcggcaacc atgccgtctg aaatccccgt
     1381 cagttccgcc acaaacttcg gaatcggttt ttgaggattg accagccatt catacctctc
     1441 cgcccttccc tgcccaaact tgaccaaagc cacttcggtt accctgtctt catacagatt
     1501 gccgcccgtc gattccaaat caaccacggc aacaggcatt ccaaaccgta aaaacgcctt
     1561 ttccagcaag ggccagcggg aaacaacaat cattttattc tctttaaatt caaacaacaa
     1621 accgatattt tacactttta aggcatttca tccaacaaaa taattgacag aatccgatga
     1681 ttaccataaa attcaaaaat ttattgca
//
LOCUS       DAXDSD010000175         1702 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXDSD010000175 DAXDSD010000000
VERSION     DAXDSD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.175"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..216)
                     /locus_tag="ACFB2Q_002099"
     CDS             complement(<1..216)
                     /locus_tag="ACFB2Q_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC0919041.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLSAVVGQTWRNLDILIVDDGSTD
                     GTPAIARHFQEQDGRIRIISNPRNLGFI"
     gene            complement(276..1196)
                     /locus_tag="ACFB2Q_002100"
     CDS             complement(276..1196)
                     /locus_tag="ACFB2Q_002100"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFC0919042.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEEN
                     RAAVAANLRGGGNIRFIDVNPEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLD
                     TDVLVRDGLKPLWDTDLGGNWVGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINL
                     KKWRRHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMAN
                     GFASRHTDPLYLDRTNTAMPVAVSHYCGSAKPWHRDCTVWGAERFTELAGSLTTVPEE
                     WRGKLAVPPTKHMLQRWRKKLSARFLRKIY"
     gene            complement(1208..>1702)
                     /locus_tag="ACFB2Q_002101"
     CDS             complement(1208..>1702)
                     /locus_tag="ACFB2Q_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC0919043.1"
                     /translation="AFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHCGTAGYIIS
                     RKAMRFFLDRFAVLPPERLHPVDLMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQ
                     NSALGSLIEHDRRLNRKQQRRDSPANTFKHRLIRALTKIGREREKRRKRREQTIGKII
                     VPFQ"
BASE COUNT          375 a          469 c          444 g          414 t
ORIGIN      
        1 gataaagccc aaattgcggg gattggaaat tatcctgatc ctgccgtcct gttcttggaa
       61 atgccgggca atggcgggcg tgccgtccgt cgagccgtca tcgacaatca aaatatccaa
      121 gttgcgccaa gtctgcccca ctacggcgga cagcgattgg gcaaaatatt tttctacgtt
      181 gtaggcgcaa atcaatacgc tgactaaagg ctgcaatccg tttctccttt ccgatacgtc
      241 cgatgccgtc tgaaggcttc agacggcctg ccccgtcaat aaatcttgcg taagaatctg
      301 gcagacagct ttttgcgcca tctttgaagc atatgctttg tcggcgggac ggcaagtttg
      361 ccgcgccatt cttcgggaac ggtcgtcagg ctgccggcca actctgtgaa acgttccgca
      421 ccccaaacgg tgcagtccct gtgccacggc tttgccgagc cgcaataatg gctgacggcg
      481 acgggcatcg ccgtattggt acggtcgagg taaagcgggt cggtatggcg ggacgcaaac
      541 ccgttcgcca taaaggcata attggtcggc ataaagttga aacggctgtt cgcataacac
      601 accccgcctt taaacagccc gttcaaaatg tcctgatcct gatattgcat cacgtccttg
      661 tattgttcca cccattcgca ggacattttg aaaatatcgt gccgccgcca ctttttcagg
      721 ttgatcagca atacgccggc attgaaataa tattctccgt ccgccatacc gattttttgt
      781 ttgtatcctt cctgcctttc gacaaacaaa tcgatgcacg cgccgaccca gttaccgccc
      841 aaatcggtat cccataaggg cttcaggccg tccctgacca atacgtccgt atccagatac
      901 aggactttgt cgcaatcggc aatgtattcg cccaatttca ggcgggcata agtcgtaatg
      961 gaaatgtgcc tgatgtttaa ggggaagccg gcgaaatctt cggggtttac gtctataaag
     1021 cggatattac cccccccccg caaattggcg gcaaccgccg cccggttttc ctcactgatg
     1081 ccggcatcga ggacgtggaa cctgatttcc gtatcgggat gggccgcttc cacgcttttt
     1141 gccgcaacgc aaaggtaggc ggcatagttg tcgtctgccg caaatacgat gtccatcttt
     1201 tctcctttta ttggaaaggc acaataatct tgccgattgt ctgttcgcgc cttttccggc
     1261 gtttttccct ttccctgccg attttggtca aggcgcggat caggcggtgt ttgaatgtgt
     1321 tggcggggga atcgcgcctt tgctgtttgc ggttcaggcg gcggtcatgt tcgatcaggc
     1381 tgcccaatgc gctgttttgg tcgtgaaact tggcataatg cagctcttgg gcgcacaagg
     1441 cgggattgag ctggcaaacc ggcattcctt ccctgtcgtc agggttgccg aacatcatca
     1501 aatcgacagg gtgcaggcgt tcgggcggca aaacggcaaa cctgtccaag aaaaaacgca
     1561 tcgcctttcg ggaaataata tagccagccg tcccacaatg ttcgctttcc aaaagcggaa
     1621 aggcgcgccc gccgtagtcc gccacgccgg agggcgaggt caggacgtgc ataaacatcg
     1681 tttccaaacg gacgataaag gc
//
LOCUS       DAXDSD010000176         1665 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXDSD010000176 DAXDSD010000000
VERSION     DAXDSD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.176"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            1..219
                     /locus_tag="ACFB2Q_002102"
                     /pseudo
     CDS             1..219
                     /locus_tag="ACFB2Q_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360925.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            308..877
                     /gene="rsmD"
                     /locus_tag="ACFB2Q_002103"
     CDS             308..877
                     /gene="rsmD"
                     /locus_tag="ACFB2Q_002103"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC0919044.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRNAKRVVIADNNRQTVQTLEKNSRELGLG
                     QVQTVCSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            1000..1251
                     /locus_tag="ACFB2Q_002104"
     CDS             1000..1251
                     /locus_tag="ACFB2Q_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC0919045.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            1348..1431
                     /locus_tag="ACFB2Q_002105"
     tRNA            1348..1431
                     /locus_tag="ACFB2Q_002105"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1382..1384,aa:Tyr,seq:gta)
     gene            1462..1535
                     /locus_tag="ACFB2Q_002106"
     tRNA            1462..1535
                     /locus_tag="ACFB2Q_002106"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1494..1496,aa:Gly,seq:tcc)
     gene            1545..1619
                     /locus_tag="ACFB2Q_002107"
     tRNA            1545..1619
                     /locus_tag="ACFB2Q_002107"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1577..1579,aa:Thr,seq:ggt)
BASE COUNT          467 a          365 c          435 g          398 t
ORIGIN      
        1 aatccggatt tgtcgggatt cgctcaaatc gggcggcggc gggatggtgt tgcagattaa
       61 cgatgtggat accaacagcg gggaatttgc aatctatacc gctcaggatg catcggtaaa
      121 gctggatttg ttaaaaatgg aattgaaaca ctgcaaagaa atgttggaac aaaagacaaa
      181 gaaatcgagt tgctccgcaa gctgaccgaa accgtctaaa cagatatgcc gtctgaaaaa
      241 agttttcagg cggcatattc ttcgacaggt cttgtataat accgtttgaa ctttcaggct
      301 tttgattatg gcggcaggca aacataccaa acacagcaac cgggtacgca ttatcggcgg
      361 gcaatgccgg ggcaggaaat tgagttttgc atccgccgac ggactgcgcc caacccccga
      421 cagcgtgcgt gaaaagctgt ttaactggct gggacaggat ttgacgggaa aaacggtttt
      481 ggatctcttc ggaggcagcg gcgcactcgg catggaggcg gcatcgcgta atgcaaaacg
      541 ggtcgtgatt gcagacaaca accgtcaaac ggtgcaaacc cttgagaaaa acagccgcga
      601 actgggtttg gggcaggtgc aaaccgtctg ttcggacggc attgcctatt tggcaaacct
      661 aaaagagaaa ttcgatgtcg tcttccttga tccgccgttt gcgtggcaaa gttgggaaag
      721 tcttttcaat gtattgggca cacgcctgaa tgacggcgca tatgtttata tcgaggcagg
      781 caggcagccg gacaaacctg attggctgac gggatatagg gaggggaagt cggggcaggg
      841 tacatttgaa ttaagggttt tccaagtggc tgaataatat gcgctttgat aatcatttcc
      901 gggttgtaaa cattcgtttg caaccgtccg gttcaaaaaa accttgtgct ataatccgcg
      961 cccgcccggt tttgataatt tagtggaaaa ggaaaagaaa tgtcgctttt tattaccgac
     1021 gagtgcatca actgcgacgt atgcgaaccc gaatgcccca atgatgccat ttcccaaggc
     1081 gaggaaattt acgaaatcaa ccccaacctc tgcacgcagt gcgtcggaca ctacgatgag
     1141 ccgcagtgcc agcaggtttg cccggtggac tgcatcctga ttgacgaaga acatcccgaa
     1201 acccatgacg agttgatggc gaaatacgaa aagattatcc agtttaaata aattcttttt
     1261 aaaacatcaa attatgtctg ttttgaaata aaatcaaaaa aaaacttgac ggaaaagcaa
     1321 gccgctaata aactagtgtt ctcttttgga gggattcccg agcggtcaaa gggggcagac
     1381 tgtaaatctg ttgcgaaagc ttcgaaggtt cgaatccttc tccctccacc aaaattctta
     1441 cttggggcag tagcgagtaa tgcgggtgta gctcaatggt agagcagaag ccttccaagc
     1501 ttacggtgag ggttcgattc ccttcacccg ctccaaacaa ttaggcccat gtagctcagg
     1561 ggtagagcac tcccttggta agggagaggt cggcagttca aatctgccca tgggcaccat
     1621 ctctcgatta ttcatttctt taaggcttag atatatagga tattg
//
LOCUS       DAXDSD010000177         1636 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXDSD010000177 DAXDSD010000000
VERSION     DAXDSD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.177"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(62..1030)
                     /gene="ispH"
                     /locus_tag="ACFB2Q_002108"
                     /gene_synonym="lytB"
     CDS             complement(62..1030)
                     /gene="ispH"
                     /locus_tag="ACFB2Q_002108"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC0919046.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGGGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1060..1554)
                     /gene="lspA"
                     /locus_tag="ACFB2Q_002109"
     CDS             complement(1060..1554)
                     /gene="lspA"
                     /locus_tag="ACFB2Q_002109"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC0919047.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          355 a          496 c          410 g          375 t
ORIGIN      
        1 ttttgcaaag gtctcggcat ttgtgttgcc caaacatccg atgcctgcgt taacgcctgc
       61 gtcaacgttt gcacaaatcg ggtttggttt cgccctcgcg gcgcagctcc ttgggcagga
      121 cgaacacgat gctttcttcc gcgccccccc cttcgcgcac ggtttcatgc ccccatccgc
      181 gtatggttgc caatacttcc cgcaccaaca cttcgggcgc ggacgcgcct gccgttacgc
      241 cgactttgct tttgccttca aaccacgtgc gttgcaggta ggacgcgttg tccaccatat
      301 acgcatcgat tccgcgcgat gccgccactt cgcgcaggcg gttgctgttg gacgaattgg
      361 gcgaaccgac cacaatcacg atgtcgcact gttccgccag ctctttgacg gcggtttgcc
      421 ggttggtcgt cgcatagcag atgtcttcct tgtgcggatt gcggatattg gggaaacgcg
      481 cgttcagcgc ggcgatgatg tctttggttt catcgaccga gagcgtggtt tggctgacat
      541 aggcgagttt gtcggggttt ctgacttcga gttttgccac atctccgacc gtttcgacca
      601 aaagcatttt gcccggtgca agctgcccca tcgtgccttc gacctcggcg tgccccttat
      661 gcccgatcat gatgatttca cagtcttggg catccagtcg ggcgacttcc ttatgcactt
      721 tcgtcaccag cgggcaagtc gcatcaaata cccggaaacc gcgctccgcc gcttcctgct
      781 gcaccgcctt cgatacgccg tgtgccgaat aaaccagtgt cgcgcccggc ggcacttccg
      841 ccaagtcttc gataaacacc gcgccttttt cgcgcaggtt gtccacgacg aatttgttgt
      901 ggacgacttc gtggcgcaca taaaccggcg cgccgaattc ttccaaagca cgttcgacaa
      961 tactgattgc ccgatccaca ccggcgcaga agccgcgcgg attggcaagg atgatggttt
     1021 ttccgttcat aagttttgca ttccgtgttc agacggcatt cacgtttttt tgctatcttt
     1081 gcgatggacg atattgtcaa gcaccgccaa caccgcaccg acgcagataa agctgtcggc
     1141 aatattaaag gcgggataaa accaattttg ccaataaaac aataagaaat cgacgacatg
     1201 accgtgtatc aggcgatcga tgacattgcc cgacgcaccg ccgataatca ttgccgcccc
     1261 gattttgccg agggctgcaa actcgtcgcg caagatggcg cgtaccaaat acgcgctcac
     1321 cgccaccgcc agcaccaaaa aaaagtattt ctgccagccg ccctgatcgg caaggaagct
     1381 gaatgccgcg cccggattgt acaccagcgt cagatcgaaa aacgaaggga tgacgttgac
     1441 gcgttcccga tactgaaacg acgacagcac cgcccacttc gaccactggt ccagcacgat
     1501 ggcggcaagt gccaataccc aatagcgcgt tttacttgaa acagatgaag acatattttt
     1561 caacagccgg ataaaagagt gccattctac ccgaaaaccc cctttcctgt acccgaaacg
     1621 gcaaatgccg taatct
//
LOCUS       DAXDSD010000178         1618 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXDSD010000178 DAXDSD010000000
VERSION     DAXDSD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.178"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>1618
                     /gene="pilC"
                     /locus_tag="ACFB2Q_002110"
     CDS             <1..>1618
                     /gene="pilC"
                     /locus_tag="ACFB2Q_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC0919048.1"
                     /translation="YAIVMNERNQPEVKWNGQYQKSQLREKDGERKFIYTNQKNQWGQ
                     QNNFISFDNTDTLVSRQSGTAVFGTATYLPPYGKVSGFDTAELNKRGNAVNWIHTTRA
                     GLSGYSYTGVTCRSSKCPQLVYKTRFSFDNTGLAKNTGRLDRHPDPSRENSPIYKLKD
                     HPWLGVSFNLGAEGTDKDGRSSNKLVSSFDENNSNQNLVYTTEGHRISLGDWQREHTA
                     MAYYLNAKLHLLDKKQIENIAPGKTVRLGTLRPRVETTGRSWLDFWATWKIEDKGNIT
                     VRLDLPEVKAGRCINKPNPNKNTKAPSPALTAPALWFGPVQNGKVQMYSASVSTYPGS
                     SSSRIFLQELKTQTDPARPGRHSLAALDTQNIKSREPNFNSRQTVIRLPGGVYKINPV
                     KNGGRVAGFNGNDGKNDTFGIYKDRLVTPEADEWSEVLLPWTARYYGNDDIFKTFNQP
                     NNKKQSDKKQYSQKYRIRTKEDDNDKPRDLGDIVNSPITAVGGYLATSANDGMVHIFK
                     KTGTDQRGYELKLSYIPGTMERKDIENQDST"
BASE COUNT          484 a          449 c          385 g          300 t
ORIGIN      
        1 aatacgctat tgtcatgaac gaacgaaatc agccggaggt aaagtggaat gggcaatatc
       61 aaaaatcaca attaagggaa aaagacgggg aacgcaaatt tatctatacg aaccagaaaa
      121 accagtgggg ccaacaaaac aattttatct cattcgacaa taccgatacc cttgtttccc
      181 gacaaagcgg tactgccgtt tttggcacag ccacctacct gccgccctac ggcaaggttt
      241 ccggctttga taccgccgag ctgaacaagc gcggcaatgc cgtcaattgg attcatacca
      301 cccgggccgg gctgtcaggc tactcctaca ccggcgtcac atgcagaagc agcaaatgcc
      361 cccaacttgt ctataaaacc cgattttcct tcgacaacac cggtttggca aaaaatacgg
      421 gcaggctgga taggcacccg gacccaagcc gcgaaaattc gcccatttac aaattgaaag
      481 atcatccatg gttgggcgta tctttcaatt tgggcgccga gggtaccgac aaagatggca
      541 gatcatccaa caaattggta tcttcttttg atgaaaacaa tagtaatcaa aacctcgtct
      601 atacgacaga aggccaccgt atttcccttg gcgactggca gcgcgaacat accgccatgg
      661 cctactatct gaacgccaaa ctgcacctgc tggataaaaa acagattgaa aatatcgcgc
      721 caggcaaaac agtgcggttg ggtaccttga gaccgcgcgt cgagacgaca ggacgaagct
      781 ggctagattt ttgggctacg tggaagattg aagataaagg gaacattaca gtccgcctcg
      841 acctgccgga agtcaaagca ggccgctgca tcaataaacc gaaccccaat aagaatacca
      901 aagccccttc gccggccctg accgcccccg cgctgtggtt cggccctgtg caaaatggca
      961 aggtgcagat gtattccgct tcggtttcta cctaccccgg cagttcgagc agccgcatct
     1021 tcctccaaga gctgaaaact caaaccgacc ccgcccggcc cggccggcat tccctcgccg
     1081 ctttggatac gcagaacatc aaaagccgcg agccgaattt caactcaagg cagaccgtga
     1141 tccgattgcc gggcggcgtg tacaagatca acccggtcaa gaacggcggc cgggtcgcgg
     1201 gttttaatgg caatgacggc aaaaacgaca ctttcggcat ctacaaggac aggctcgtca
     1261 cacctgaggc cgacgagtgg agcgaagtgc tgctgccttg gacggctcgg tattacggta
     1321 atgacgatat atttaaaaca ttcaaccaac caaacaacaa aaaacaaagc gacaaaaaac
     1381 aatacagcca aaaataccgc atccgcacaa aagaagatga caatgacaaa ccccgcgatt
     1441 tgggcgacat cgtcaacagc ccgataacgg cggtcggcgg gtatctggca acttctgcca
     1501 acgacgggat ggtgcatatc ttcaaaaaaa ccggcacaga ccaacgaggc tacgaactga
     1561 agctcagcta catccccggt acgatggagc gtaaggatat tgaaaaccaa gactccac
//
LOCUS       DAXDSD010000179         1542 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXDSD010000179 DAXDSD010000000
VERSION     DAXDSD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.179"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..333
                     /locus_tag="ACFB2Q_002111"
     CDS             <1..333
                     /locus_tag="ACFB2Q_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC0919049.1"
                     /translation="ADWHTYFVKGSQAETEGVWVHNDCPPKPKPTNHAQQRKEEAKND
                     SHRSVGDSNRVVREGKQYLDSDTGNHVYVKGDKVVILTPDGRQVTQFKNSKANTSKRV
                     KNGKWTPK"
     gene            311..592
                     /locus_tag="ACFB2Q_002112"
     CDS             311..592
                     /locus_tag="ACFB2Q_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919050.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            648..1295
                     /locus_tag="ACFB2Q_002113"
                     /pseudo
     CDS             648..1295
                     /locus_tag="ACFB2Q_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1292..>1542
                     /locus_tag="ACFB2Q_002114"
     CDS             1292..>1542
                     /locus_tag="ACFB2Q_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919051.1"
                     /translation="MKTSTIVFGGFFITDNGERIQIPILENPNIKEINNFFSVSNFEK
                     KAGVLVFRIIPEPEFGNTELTIYFEKGYYLPIIQTILEDG"
BASE COUNT          538 a          272 c          315 g          417 t
ORIGIN      
        1 gccgactggc atacttattt cgtcaagggt agtcaggcgg aaacggaagg agtttgggtt
       61 cataatgatt gtccgcctaa accaaaacca accaatcatg cccaacaaag aaaagaagaa
      121 gctaaaaacg attctcatcg aagtgtggga gattccaatc gtgtcgttcg cgaaggaaag
      181 caatatttag attccgacac aggaaaccat gtttatgtaa aaggagataa agtggttatt
      241 ctaactcctg atggaagaca ggtaactcaa tttaagaact cgaaagccaa tacgtcaaaa
      301 agggtaaaaa atgggaaatg gacaccaaaa taatcaatct tccttagaaa aaagaatttt
      361 ttatctggag cattcggggc agtatctgat gatttgcgcc ctatcggatt actcccaaaa
      421 taaacatact gtcgttatgg cgaatttcct ctatccaaat gaaaaaatgg attggagaaa
      481 tttagatgat ttgttcaatg agttggtttt agaggaatta cagtcttcat ttatggattg
      541 gtatccaact attgaaaagg caattagcca tcatttagaa gacttttcgt aacaagcata
      601 aatcaaaaag tcgtctgaaa ctgcttttaa ttttcagacg acctttcccc atatcggcgg
      661 attatgaatt tacagctatc gggaaattga gttatctctc tgatagagaa aattccatac
      721 gcgaagccgt tgaccggtgg atacaggaaa atcccaatgc cgccgaaacc gtcgaagccg
      781 tcttcaacgt tgccgccgcc aaagtcgcga agctggcaaa ggcggcgaaa ccggggaaag
      841 ctgcggttag cggggatttt tcgattagct ataaaaactt ttctactgtc aaaccaaaag
      901 taattgccaa aggaacgatt aatggaaaga cttttagaga tgttaatcaa agtgcaaaaa
      961 ttggatcgcc tgattcacct acattaatag cccaacgagt taatgcaaaa atccaagccg
     1021 acggtaaacc tcgtccgaat gcaaccgttg ctaattcaca tgctgaaatt ggtgttatcc
     1081 agcaggctta taatgcaggt gaaacgaaag gggcatctat gacaatgaca gtttcaggaa
     1141 aagatgtttg cggttattgt aaaggagata ttgcagctgc tgctcaagct tctggactga
     1201 aatcactgac tgttaatgcg acagataatg taacaggaaa aaataaaact tactattgga
     1261 cgcctggtat gaaatcaatt aaggagagaa aatgaaaaca tcaacaattg tttttggtgg
     1321 attttttatt acagataatg gagaacgaat ccaaatccct attttagaaa atcctaacat
     1381 taaagaaata aataactttt tttctgtatc aaattttgaa aaaaaagccg gagtccttgt
     1441 tttcagaatt attcctgagc ctgaatttgg caatactgaa ttaactatct attttgaaaa
     1501 aggatattat ttaccaataa ttcaaacaat tttagaagat gg
//
LOCUS       DAXDSD010000180         1532 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXDSD010000180 DAXDSD010000000
VERSION     DAXDSD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.180"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(28..1014)
                     /locus_tag="ACFB2Q_002115"
     CDS             complement(28..1014)
                     /locus_tag="ACFB2Q_002115"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFC0919052.1"
                     /translation="MPNDLVVVKANSLIEANYRLSIDEIRILALTIGTMDPKSNQKIF
                     DFTVADFVREFPEISQDNAYKQIQAAIKRIYDRSVKTEDKDRVTEFRWVSSRTYFKKE
                     GRFRIAMTDEVMPYLTQLKGQFTQYQLKHIAYFNSVHSIRIYELITQYRSVGSREITV
                     EKLKEWLQVENKYPRFNSLNQRVLEPAITEINEKSDLVVEVEQIKRGRTIHSLNFVIG
                     SKKRTAQKIEEVAKRPVFPHKNKYGKFVKLDKQNPKMSNHEYGLWARDCLKILEDHYT
                     DITKVTNEDLRNYWVFLAGNDSNRSKLGSKSDFLNELKKRGYKLVDCELVKI"
     gene            complement(1347..>1532)
                     /locus_tag="ACFB2Q_002116"
     CDS             complement(1347..>1532)
                     /locus_tag="ACFB2Q_002116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919053.1"
                     /translation="YLKWQHKKRIDRMLDEMEKSYSKFADHKANERMQKRIDEYDKRH
                     PLKAFKRKFKDKIKWLL"
BASE COUNT          463 a          282 c          232 g          555 t
ORIGIN      
        1 caaaaaaaag ttaagtaaaa tacctaccta aatttttact agttcgcaat ctacgagctt
       61 ataacctcgt tttttcaatt catttaaaaa atcagatttt gagcctaatt ttgatctatt
      121 gctatcgtta cccgctagaa atacccagta attacgcaaa tcttcattgg taactttcgt
      181 aatatcggtg taatgatctt cgagtatttt taagcaatct ctagcccata aaccgtactc
      241 gtgattgctc atcttagggt tttgcttatc gagtttgacg aacttcccat acttgttttt
      301 atgtggaaat actggccgtt ttgcaacttc ttcaattttt tgagctgttc gttttttact
      361 accaatcaca aaatttaaag agtgaatagt acgcccacgc ttgatttgtt caacctcaac
      421 gactaaatca gatttctcgt taatctcagt tattgcaggt tccaaaacac gttgatttaa
      481 tgaattaaat ctagggtatt tattttcaac ctgaagccat tcttttagtt tttctactgt
      541 aatttcacga ctaccaacag agcgatattg tgtaattagc tcataaattc gaattgaatg
      601 tacactgttg aaataagcga tatgtttgag ttgatattgc gtgaattgcc ctttaagttg
      661 cgttaggtat ggcataactt catcagtcat tgcaattcta aaacgcccct ctttcttgaa
      721 atatgttcta gaggaaaccc aacgaaattc agttacacgg tctttatctt cagttttaac
      781 acttcggtca taaatccgtt ttatagccgc ctgaatttgc ttataggcgt tatcttggct
      841 tatttctgga aactcacgga caaaatcagc caccgtaaaa tcaaaaatct tttgattaga
      901 tttcggatcc atagtcccaa tagttaaagc taaaattctg atttcatcaa tactcaatcg
      961 gtaattggct tcaataaggc tattagcctt tacaacaact aaatcatttg gcataagaca
     1021 acaaatttcc tgtttaaaac aacaagcaaa atatacctgt tgtttatata taaaacaaca
     1081 agtattttct taaaagttgt ctataacagg aaatttgttg tcgtataagt ttgtaactta
     1141 ttgattttac tggttttaaa aacgccgaaa acaagtaaaa aacaaaaata taaaaatata
     1201 gggactttcg tccctttttt gggctttcag ccctaatttt ttctttttca ggattaaaaa
     1261 ttacaaaacc cttacagagc aagtaaactt gtttgcttgt tctgcaaggg ttcagcaacc
     1321 gaagccgtta ggcgtagggc ggtagcctat aaaagccatt taattttatc tttaaatttc
     1381 cgtttaaatg ctttgagtgg gtgtctttta tcgtactcat caatcctttt ttgcattctt
     1441 tcgtttgctt tgtgatcggc aaattttgaa taagattttt ccatctcatc taacattcta
     1501 tcaatccgtt ttttatgttg ccatttcagg ta
//
LOCUS       DAXDSD010000181         1475 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXDSD010000181 DAXDSD010000000
VERSION     DAXDSD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.181"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..158)
                     /locus_tag="ACFB2Q_002117"
     CDS             complement(<1..158)
                     /locus_tag="ACFB2Q_002117"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HFC0919054.1"
                     /translation="MALGTSANASNFGISLGKSSAASGTKGIAVGTSSQATNLSAVAI
                     GTESKAQN"
     gene            444..788
                     /locus_tag="ACFB2Q_002118"
     CDS             444..788
                     /locus_tag="ACFB2Q_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFC0919055.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSNGARVRDLQGNLLYSNSLPEELVLELYKTSYVG
                     NLIQIPRFCRHQ"
     gene            742..1395
                     /locus_tag="ACFB2Q_002119"
                     /pseudo
     CDS             742..1395
                     /locus_tag="ACFB2Q_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
BASE COUNT          450 a          304 c          303 g          418 t
ORIGIN      
        1 ttattttgtg ccttactttc agtaccaatt gccaccgcac ttaaatttgt tgcctgagaa
       61 gatgtcccca ctgcaattcc tttcgtacca ctggcagcac tgcttttccc taaggaaata
      121 ccaaaattcg atgcattagc agaagtaccc aatgccactc aattttagat gccgatgctt
      181 tatcgttcca tgataaggag ccattattac cttcttgcaa agtagaatcc gccccataag
      241 aagacaaagc actcattaac aaaataccag ccgctatcgc ggacgaccta tatacataac
      301 attacttcat atcatttcat tcagtatcaa tagtatagga taactccccc ccccgtcaat
      361 cgaaataatt cccgtatttt aatgtcgtat tgttcctgta tatttagtga tgtgataagt
      421 atagtttatt ttttgaggtt tttatgaatt taccttttcg ggcaatggtg tctgatttag
      481 gtggcacact tttaacacct gaacatttag tgggtgatct taccattgat actctgagag
      541 tattagagca aaaaggcgta gatattattt tggcaacagg acgcaatcat accgatatgt
      601 catccattct tggaaaaatc ggtgcggaac gtgcggttat gattacatca aacggtgctc
      661 gtgtacggga tttacagggt aatttgcttt atagcaatag tttgcctgaa gaattagttc
      721 tcgagcttta caaaacatca tatgtgggaa atcttattca gataccaaga ttttgtcgcc
      781 atcaataaac ctcaaggcat atccgtccac cgttccggcg gcgaagtcag tctgacggca
      841 acacttgcgg cacagttggg cgtagaaaag gtatggttgc tgcacaggtt ggacaagcag
      901 gccggaggca tattgctgtt tgcccttaat ccccaaagtg ccgctgttct tgccgcacag
      961 tttgccgaaa ggaaaatgaa gaaaacctat ttggctttgt ccgaccgcaa accgtccaaa
     1021 aaacaaggat ggataaaagg cggcatggaa aaatccaggc gcgggatgtg gaagctgacg
     1081 cgcaatatgg aaaatattgc cgttacccgg tttttcagta tccgcatcag tgaaaaaatg
     1141 cggctgttca tccttgagcc acatacgggt aagacacatc agttgagggt ggtaatgaag
     1201 agtctgggca gctctatatt tggggacagc ctgtatggag gaacagaatc cgaaactatg
     1261 ttcctgtatg cgtgaaaaat acaatttacc tatcaacaac aagaaatcga aattaccgca
     1321 cctttgaaaa atgcatggca ggccgaaaat atctctcatg cactggaaga attttatatg
     1381 gcaaaagcag gttaatagat ataaatcaat taaaacaata tgataaatat ttttataaaa
     1441 ttttttcaaa aaacggttga ccgcaaatct cttta
//
LOCUS       DAXDSD010000182         1373 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXDSD010000182 DAXDSD010000000
VERSION     DAXDSD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.182"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..1195
                     /locus_tag="ACFB2Q_002120"
     CDS             <1..1195
                     /locus_tag="ACFB2Q_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004466861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC0919056.1"
                     /translation="AAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLWGISGKYLYSP
                     WQWCRLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVVKISPDKEVSG
                     YGIPAIDVNFYRVFMQHPQIVSRKETAAAPRLLSGPEGRLLYTKDARVYTKGLKEPGR
                     YLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEERLKDNEYYTR
                     THPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEPSRPVQAKIVS
                     VFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLTEEPKSRDTVE
                     LISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDNMPDQGRARVD
                     SDPFQ"
BASE COUNT          348 a          400 c          352 g          273 t
ORIGIN      
        1 cgcggcaggt atggcattct caacacaaac tttggcggca aatctggagg tgcgcccgga
       61 tgcgccgcaa cgctacacgg tcaaacaggg cgacaccctg tggggtattt cgggcaaata
      121 cctgtacagc ccgtggcaat ggtgccgcct gtggggcgcg aaccgggatc aaatccacaa
      181 tcccgacctg atttatccgg gccaggtatt ggtgttgcgc tacgttggcg gcgaaccgcg
      241 ccttgatttg gaacagacag acggcatccc cgtcgtcaaa atcagtccgg ataaggaagt
      301 gtccggatac ggtattcccg ccatcgatgt caacttctac cgcgtcttta tgcagcaccc
      361 gcaaatcgtt tcccgcaaag aaaccgctgc cgcgccgcgc ctgctctcgg gcccggaagg
      421 caggctgctg tacaccaaag acgccagggt ttacaccaaa ggcctgaaag agccgggccg
      481 ttatctgacc taccgaatca ataaaaacat caccgatccg gatacgggta aattcctcgg
      541 acaagaagtc gcattcagcg gcatcgtgcg ctccctcgac tataccgact ccgccctcga
      601 acaacgctcg aaacaggctg aagaacggct gaaagacaac gaatactaca cccgcaccca
      661 tcctttgatt accccggtgc gcacccgttc gatacagccg ctggtggtcg aaaccgccat
      721 ttccgaaata cagcagggcg actacctgat gaaaatgccg gaagacaccg accgcttcaa
      781 tatggtgccg cacgaaccgt cccgcccggt tcaggcaaaa atcgtttccg tgtttgaagg
      841 cgtgggggtc ggcggacagt tcaaaaccat tacgatagac aaaggcgggg acgacggttt
      901 ggacaaaggc gcagttttaa gcctttacaa acgcaagaaa acgatgcagg tcaacctctc
      961 caacaatttg accgaagaac ctaaaagcag ggataccgtc gagctgattt cgacacctgc
     1021 cgaagaagtg ggcttggcaa tggtttacca cactgcgccg aaactggctt acgccattat
     1081 tttggaaaac atctccgata tttccgaagg cgacaccgcc gccaatccgg gacgggattt
     1141 ggacaatatg ccggatcagg gccgcgcccg cgtcgattcc gaccctttcc aataacccct
     1201 cggcaacaat gccgtctgaa gcattcagac ggcattttta tttaggctgt gttttcatat
     1261 cccgttgatg caaaaccctg atttagtcgg acaaaatgcc ttttatccga tggaatcggt
     1321 ttccgcccga acggaaatca cagcggctgt ctcccttgca gacagatata gcg
//
LOCUS       DAXDSD010000183         1323 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXDSD010000183 DAXDSD010000000
VERSION     DAXDSD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.183"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(92..1015)
                     /gene="lpxC"
                     /locus_tag="ACFB2Q_002121"
     CDS             complement(92..1015)
                     /gene="lpxC"
                     /locus_tag="ACFB2Q_002121"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC0919057.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAVNNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          315 a          316 c          363 g          329 t
ORIGIN      
        1 aaaacggttt cttgagattt tgcgttccgg attcccgctt tcgcgggaat gacggcatct
       61 gtttgacggc atttgtttgg aaaagggggc gttatccgca atttctgatg ttcagctcgt
      121 gaaatgcgtc gggcaaatca tcgctgtcgg caaattccac ccattcgtaa gccgtttcgt
      181 ctgccaaaac tgcgcgcaaa agggcgttgt tgacggcgtg tcccgatttg tagccttcaa
      241 acgcgccgac aatcggatgt ccgacgatat acaaatcgcc gatggcgtcg aggattttgt
      301 ggcgcacaaa ctcatcggga tagcgcaagc cttcaggatt caggacatcc gtgtcgccaa
      361 tcacgatggc gttgttcaaa ttgccgccta agccgagatt gtgggcgcgc atcatttcca
      421 cttcgtgcat aaagccgaaa gtgcgcgcgc gcgcgatttc gccgatgtag gatttgccgg
      481 cgaaatcaat ttcaaacgta ggcgggctgc ggttgaaaac aggatggtcg aattcgatgg
      541 tcagcgttac cttaaaaccg tcatacggcg taaatcgaac ccatttgccc gcttctttga
      601 tttcgacggg tttgaggatt ttcaaaaaac gcttttgcgc cttttggtct acaacgcccg
      661 catcttgcag gaggtaaata aacggcaggc tggagccgtc cataatcggg atttcgggcg
      721 cgttcaactc aatcagcgca ttgtcgatgc cgtaggcgga cagtgcggac atgatgtgtt
      781 cgatggtgcc gacgcgcagg cctttgtcgg taacgatggt ggatgaaagg cgggtatcgt
      841 tgatcaaata agggttcagc ttgatttgtt cgcccatctc gccgtccaaa tcggtgcggc
      901 ggaaggaaat cccgctgttt tcaggcgcag ggtgcagggt cagcgcgacg cgttcgcccg
      961 aatgcagccc gactccggta acgccgatgg atttcgccaa agttctttgc agcataaacc
     1021 gcttccttat caatggggta agtttcggaa taatacgata aaaccggaaa aacaggctat
     1081 gtttttcaat agtatttgcc aatgtatccg tttccaacac gtaagccgca tgaaaatgta
     1141 tggcggatta acgaaaatca ggacaaggcg gcgggccgca ggcagtacgg atagtacgaa
     1201 aaggcgaggc aacgccgtac tggtttaaat ttaattcact atacatcccg tcattcccgc
     1261 gcgggcggga atctagaatc ccggactttc agataatctt tgaatattgc tgttgttcta
     1321 agg
//
LOCUS       DAXDSD010000184         1280 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXDSD010000184 DAXDSD010000000
VERSION     DAXDSD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.184"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            144..449
                     /locus_tag="ACFB2Q_002122"
     CDS             144..449
                     /locus_tag="ACFB2Q_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFC0919058.1"
                     /translation="MTRQKTHPNLARYIRERVLEDGKASDKKTVKFQFPPEVVRVLAG
                     MGNNLNQIAKALNTAAKVGTLGNVEALKATTELAALERSLNSLRDFLAKEKNGWQSQ"
     gene            434..1063
                     /locus_tag="ACFB2Q_002123"
     CDS             434..1063
                     /locus_tag="ACFB2Q_002123"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFC0919059.1"
                     /translation="MAVPMIVQFFNRGKGGGSGPIDYLLGKDRDREEARLLRGDPEET
                     AALINSSDYAKKYTAGCLSFEESNIPAEQKHALMDSFEECIFAGLDKDQYNCLWVEHR
                     DKGRLELNFVIPNIELLSGKRLQPYYYAADRGRVDAWRTMQNLTHGYSDPDDPAKRQS
                     MTQAKDLPRNTQEAAQSITEHHRRLRSPSPIRQAKKPRRRAGNAGKGRF"
     gene            1147..>1280
                     /locus_tag="ACFB2Q_002124"
     CDS             1147..>1280
                     /locus_tag="ACFB2Q_002124"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFC0919060.1"
                     /translation="MSKISDLAKTFEQTSRTEAASIEQQTKADLETLRKNINEALKQS
                     "
BASE COUNT          400 a          341 c          333 g          206 t
ORIGIN      
        1 cgaaatgccg tgatgaaatg ccgttttttt gagcagaaag cagtcaaaaa caggggtatt
       61 ttgccctttt gacaggttcg agtgccgccg aaaagcgaac aaagcaactc atcatccgag
      121 tcagcccgac cgagtttgag actttgaccc gacagaagac ccatccgaat ttagcccgct
      181 acattcggga gcgggttttg gaagatggca aagcatccga caaaaaaacc gtcaaattcc
      241 aattcccgcc cgaagtcgtg cgcgtccttg caggcatggg taacaacctg aaccaaatag
      301 ccaaggccct gaacaccgcc gcaaaggtcg gcacgttggg caatgtggaa gcactcaagg
      361 cgacgaccga gctggcagcg ttggaacgtt ccttaaattc cctacgggat tttttagcca
      421 aagaaaagaa cggatggcag tcccaatgat tgtgcagttt ttcaataggg ggaaaggcgg
      481 cgggagtggt ccgatagact atcttctagg caaagaccgc gaccgagaag aagccagatt
      541 attacgcggc gaccccgaag aaaccgccgc cctgataaac agcagcgatt acgccaagaa
      601 atacaccgcc ggctgcctga gctttgaaga aagcaacatc cccgccgaac agaaacacgc
      661 cctgatggac agcttcgaag agtgtatttt tgcaggcttg gacaaagacc aatacaactg
      721 cctatgggta gaacaccgag acaaagggcg tttggaactc aacttcgtga taccgaacat
      781 cgagcttttg agcggaaagc ggttacagcc ctactactac gccgccgaca gaggaagagt
      841 ggacgcatgg cgcaccatgc agaacctgac gcacggatac agcgacccag acgaccccgc
      901 caaacggcag agcatgaccc aagccaaaga cctgccgaga aacacgcagg aagccgcaca
      961 gagcatcaca gagcatcaca gacggcttag aagccctagc cctatcaggc aagctaaaaa
     1021 gccgcgcaga cgtgctggaa acgctggaaa aggtaggttt tgaaatatca cgagcgacca
     1081 tcagcagcat cagcatcaag aacccggacc caaaagggcg caacatccga ctgaaaggcg
     1141 cactgtatga gcaagatttc cgatttggcg aagaccttcg agcagacatc acgcaccgaa
     1201 gccgccagca tagagcaaca aacgaaagca gacttagaga cgttacggaa aaatatcaac
     1261 gaggcattga agcaaagcga
//
LOCUS       DAXDSD010000185         1279 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXDSD010000185 DAXDSD010000000
VERSION     DAXDSD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.185"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(12..290)
                     /locus_tag="ACFB2Q_002125"
     CDS             complement(12..290)
                     /locus_tag="ACFB2Q_002125"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC0919061.1"
                     /translation="MAISDVFYQKQLKGNGLVQSLSRKGNCLDNAAMESFFGTLKSEC
                     FHTCKYDSVTESEAALHEYIRYYNNDRIKLKLKGLSPVQYRIQSLKAA"
     gene            complement(254..832)
                     /locus_tag="ACFB2Q_002126"
     CDS             complement(254..832)
                     /locus_tag="ACFB2Q_002126"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFC0919062.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAPNI
                     LQRCFHSEKPNEKWVTDVTEFDVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLAGEI
                     LKGAPEKPGPSEKPMLHSDQGWQYQMFFIKSS"
     gene            complement(837..>1279)
                     /locus_tag="ACFB2Q_002127"
     CDS             complement(837..>1279)
                     /locus_tag="ACFB2Q_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC0919063.1"
                     /translation="LYFSISDSLVRKWVARYRLHGESGIKRRKHTTKYSVEYKLEAIR
                     PVTGQGMSQKAAADQLNLPDCSVLPQWLRLYRLNGINGLKPKPKGRKPVKKQYPPQTK
                     KADYLKTKEELFAELAYLKAEAAVLKKLDALKEVRQKERNSSQG"
BASE COUNT          276 a          325 c          291 g          387 t
ORIGIN      
        1 ggacagttta atcaagcggc tttcagggac tgaattctgt actgaacagg gctcagtcct
       61 tttaatttca acttgattct atcgttgttg tagtaacgga tatattcgtg cagtgccgct
      121 tccgattcgg taacggaatc atatttgcac gtatggaaac attccgattt caacgttccg
      181 aagaaacttt ccattgccgc attgtccaag cagtttccct tgcgggacag actctgaacc
      241 agaccgttgc ctttcaactg cttttgataa aaaacatctg atattgccaa ccttgatccg
      301 aatgcagcat cggcttttca gacggccccg gtttctccgg cgcacctttc agtatctcgc
      361 cggccaaatc gaaagtcggg cgggtttgaa tacggtaact gacgatttcc ccgttaaaca
      421 aatccataat cggagaaagg tatatctttt ctccgcctac atcgaactcg gtaacgtccg
      481 ttacccattt ctcattcggc ttttctgaat ggaaacagcg ttgcaggatg ttcggcgcaa
      541 ctttgccgac ttctcctttg aacgagcggt atttgcgccg ccgtatcact gccttcagcc
      601 ccgtcttcgc catcagacgg ctgactttct tgtgattgac cggtattcct gcgtgacgga
      661 tggctgccgc aatcctccgg tagccgtatc ttcccttata tcgttgataa atatcatgga
      721 tatgtcgttt caaatcggca tatttgcctt ctgccgattg gacggccaat tggtaataga
      781 aggtgctgcg tggcagtccg acaatcccca acagcagttc aacggatggc atttccttaa
      841 ccctgcgacg agttgcgttc tttctgccgc acttctttca aggcatcgag cttttttagg
      901 acagccgctt ccgctttaag gtaagccaat tccgcaaaca gttcttcctt ggttttcaga
      961 tagtcggctt ttttcgtttg cggcggatac tgttttttca cgggctttct tcctttgggt
     1021 ttgggcttta aaccgttaat accattcaaa cggtagaggc gcaaccattg cggcaaaacg
     1081 gagcagtcgg gcagattcag ttggtctgcg gcagcttttt gggacattcc ctgccccgtc
     1141 accgggcgga ttgcctcaag tttgtattcg accgaatatt ttgtcgtatg ctttctgcgt
     1201 ttgatgccgc tttcaccgtg taatctgtat cttgccaccc atttgcgtac caatgaatcg
     1261 gaaatggaaa aatatagtg
//
LOCUS       DAXDSD010000186         1230 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXDSD010000186 DAXDSD010000000
VERSION     DAXDSD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.186"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>1230
                     /gene="pilC"
                     /locus_tag="ACFB2Q_002128"
     CDS             <1..>1230
                     /gene="pilC"
                     /locus_tag="ACFB2Q_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC0919064.1"
                     /translation="YAIIMNGQNQPEVKSSVPYSIKDKDRKRKYTHQNTQGGGGSVSF
                     NNSDELVSQQSGTAVFGTATYLPPYGKVSGFDTDSLKGRANAVDWIRTTRIALAGYSY
                     AGVICRNITGCPKLVYKTQFTFDQQGLKKKAGSKLDIYEDKSRENSPIYKLSDYPWLG
                     VSFNLGSENTVKDGKSFNKLISSFGEDNNNQTIVSTTEDYPISLGDGRREHTAVAYYL
                     NAKLHLLDKKQIQNITGKTVRLGVLKPSIDVRTRNTGFGFLNFWAKWDIKDNGQIPVK
                     LGLPEVKAGRCINKPNPNPKSALSPALTAPALWFGPAQNGKVQMYSASVSTYPDSSSS
                     KIFLQNLSRNDDKNKPGRYSLKSLNDGEIQSRQPSFNGRQTIIRLDDGVHLIKLNGSK
                     DEVAAFVNLNGDNTGKN"
BASE COUNT          364 a          308 c          288 g          270 t
ORIGIN      
        1 aatacgctat tatcatgaac gggcaaaacc agcccgaggt aaagtcgagt gtgccatatt
       61 caataaagga caaagacagg aagcgcaaat atactcatca gaacacacaa ggaggaggag
      121 gctctgtctc attcaacaat agcgatgagc ttgtttctca acaaagcggt actgccgttt
      181 ttggcacagc cacctacctg ccgccctacg gcaaggtttc cggttttgat accgatagtc
      241 tgaaagggcg cgccaatgcc gttgattgga ttcgtaccac ccgcatcgcg ctggcaggct
      301 acagctacgc cggtgtcata tgcagaaaca tcacaggctg tcccaaactt gtctataaaa
      361 cccaatttac cttcgatcaa caagggttga aaaaaaaggc aggcagcaag ctggatatat
      421 acgaagacaa aagccgcgaa aattcgccca tttacaaatt gtcggattat ccatggttgg
      481 gcgtgtcttt caatttgggc agcgagaata ccgtcaaaga tggcaaatca ttcaacaaat
      541 tgatatcttc ttttggtgaa gacaataata atcaaaccat tgtctctacg acagaagact
      601 accctatttc ccttggcgac gggcggcgcg aacataccgc cgtggcctat tatctgaacg
      661 ccaagctgca cctgctggat aaaaaacaga ttcaaaatat caccggcaaa acagtgcggt
      721 tgggtgtctt gaagccgagc atcgatgtgc ggacaagaaa tacggggttc ggctttctaa
      781 atttttgggc taagtgggac attaaagata acgggcagat tccggtcaag ctcggcctgc
      841 cggaagtcaa agcaggccgc tgcatcaata aaccgaaccc caatcccaaa tcagcccttt
      901 cgccggcact gaccgccccc gcgctgtggt tcggccctgc gcaaaatggc aaggtgcaga
      961 tgtattccgc ttcggtttct acctaccccg acagttcgag cagcaaaatt ttcctgcaaa
     1021 acctttcccg caatgatgac aaaaacaaac cgggccgcta ttccctcaaa tctttgaatg
     1081 atggtgagat tcaaagtcga cagccgagtt tcaacgggcg gcaaacaatc atccgattgg
     1141 atgacggcgt acatttgatc aaactgaatg gaagcaagga tgaggtcgcc gcttttgtca
     1201 atttaaatgg agacaacacc ggcaaaaacg
//
LOCUS       DAXDSD010000187         1203 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXDSD010000187 DAXDSD010000000
VERSION     DAXDSD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.187"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(1..90)
                     /locus_tag="ACFB2Q_002129"
                     /pseudo
     CDS             complement(1..90)
                     /locus_tag="ACFB2Q_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(189..404)
                     /locus_tag="ACFB2Q_002130"
     CDS             complement(189..404)
                     /locus_tag="ACFB2Q_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919065.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQLVQELEQHLIEAYNLMDTSN"
     gene            complement(528..887)
                     /locus_tag="ACFB2Q_002131"
     CDS             complement(528..887)
                     /locus_tag="ACFB2Q_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC0919066.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            complement(884..>1203)
                     /locus_tag="ACFB2Q_002132"
     CDS             complement(884..>1203)
                     /locus_tag="ACFB2Q_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919067.1"
                     /translation="RLASAVKDWKTIQPNKKGEINFGIGSATRQEAEQLGKIWVGDGA
                     KPVSSPSCQGCMLSADGTRLYRPPTTKSNTPESLNPTGVQANFVTRSVDGKTLTNGHL
                     NIK"
BASE COUNT          360 a          238 c          185 g          420 t
ORIGIN      
        1 gatgccgcga acatcatcca tacggttagc ccaccaattt ttatcggggt cgttttccca
       61 tatcagtttt cctgcttcat cagcacagtt aggattggca ttgatgccca cccagtttca
      121 ggcagcctga aaaggatttt taagatacgg ctaggtgggc tgcgagaacc cacactctgc
      181 ataaccattt agttagatgt atccattaga ttatatgctt caattaaatg ttgttctagc
      241 tcttgcacta attgaggatt gcgaattcct gacacaatct caaattcttc tgcatctatg
      301 caaacacctg cataaatttc aataacaagg gaacgcaata attgaagctc ttctcttgtt
      361 aaagaaataa taatgtcatc acctgtgtaa ttgattatat tcataataat tttatttttg
      421 cttgtcaaag taagttttac ctaaggttgg tctaaatgca gtcccaccat cttttgaatt
      481 tgggtctcta attacaattg ctccagactt atcatcccaa attgctctta tgtgtttgga
      541 ttgtaatctt cgaattccca agaaaaaaat cgtaataagt ttgaaagtgt caaatcccaa
      601 gtttcttttg agcaatattc taatatttta tcaatttcac ttttaataat cttatgatca
      661 aactgttcta atattaatgc attagaccaa aaaaaacctt ctttattaca atgatgggaa
      721 atccatttag gagaacaaat gcaaagtgaa aaaatagatg agccttgttc tccttcgatt
      781 ccgatatcca aatctatcca tctatgaaaa ttatctggaa tttcgggggt aaatttttca
      841 aaatcaatat catataaatt tatgcttttt aagtccaatt taatcatttt atattcaaat
      901 gtccattagt taaggttttt ccatcaacac ttctagtaac aaaattagct tgcactcccg
      961 ttgggttaag tgattcaggg gtgtttgatt ttgtagttgg aggacgatat agccttgtgc
     1021 catctgcact taacatacat ccttgacaag atggactact aactggctta gccccgtctc
     1081 caacccaaat ttttcctaac tgttcagcct cttgacgagt tgcagaacca ataccaaaat
     1141 taatttcccc ctttttattt ggttgtattg ttttccaatc ttttacagca cttgctaacc
     1201 ttg
//
LOCUS       DAXDSD010000188         1165 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXDSD010000188 DAXDSD010000000
VERSION     DAXDSD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.188"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..385
                     /locus_tag="ACFB2Q_002133"
     CDS             <1..385
                     /locus_tag="ACFB2Q_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC0919068.1"
                     /translation="KSITSEGKANAATYPKLVNQLNEQNLNNIAAQDPRLSLAIHEGK
                     KNFPIGTATYEEADRLGKIWVGEGARQTSGGGWLSRDGTRQYRPPTEKKSQFATTGIQ
                     ANFETYTIDSNEKRNKIKNGHLNIR"
     gene            390..749
                     /locus_tag="ACFB2Q_002134"
     CDS             390..749
                     /locus_tag="ACFB2Q_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC0919069.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            826..1035
                     /locus_tag="ACFB2Q_002135"
     CDS             826..1035
                     /locus_tag="ACFB2Q_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919070.1"
                     /translation="MKLIDFEGNLVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLLLDKELKKIYDTWDKC"
BASE COUNT          444 a          142 c          223 g          356 t
ORIGIN      
        1 taaatctata acgtcggaag gaaaagctaa tgctgcaacc tatcctaagt tggttaatca
       61 gctaaatgag caaaacttaa ataacattgc ggctcaagat ccaagattga gtctagctat
      121 tcatgagggt aaaaaaaatt ttccaatagg aactgcaact tatgaagagg cagatagact
      181 aggtaaaatt tgggttggtg agggtgcaag acaaactagt ggaggcggat ggttaagtag
      241 agatggcact cgacaatatc ggccaccaac agaaaaaaaa tcacaatttg caactacagg
      301 tattcaagca aattttgaaa cttatactat tgattcaaat gaaaaaagaa ataaaattaa
      361 aaatggacat ttaaatatta ggtaattata tgagattaaa attaaaaaat tatgattgtt
      421 ctgactttga tttggaagat ttcccacaag acaagttaga aaatttttgt atacttttga
      481 cattatcaat aggttttgat gaaagtaatg gtgctgatta tttttatgtt tatatatata
      541 gcaccgaatg gttactttca aatatacata gaccgatgtc attaaaaaac tcaattgtta
      601 caaaccgttt taatattgaa cacatattaa aattgataaa tgatatattg gaaatatgta
      661 attcaacgtc tgaagataag tctatatcaa atttggcaaa atattttgac tgggaatttg
      721 acgattataa tctaaatatc caagactaaa gatggtggga cagcatttag accaaaaaca
      781 ggtaaacagt attttgatga caaattaaaa taaaaggaat tattcatgaa attaattgat
      841 tttgaaggga atttagttaa aattagtctc gataaagatg agttgtatat tattcaagca
      901 attgtaggag aaatttattc aggcgtatgc gtggattgta gagattttga gataattcat
      961 ggtgttgaaa aaaataaagt tcttcttttg gataaagaac ttaaaaaaat atatgatact
     1021 tgggataaat gttagctaga tagggtgggc actcgttgcc caccttttgc cgtatattaa
     1081 aaatcctttt caggctgcct gaaactgggt gggcatcaat gccaatccta actgtgcgga
     1141 tgaagcagga aaactgatat gggaa
//
LOCUS       DAXDSD010000189         1135 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXDSD010000189 DAXDSD010000000
VERSION     DAXDSD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.189"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(143..862)
                     /gene="pyrH"
                     /locus_tag="ACFB2Q_002136"
     CDS             complement(143..862)
                     /gene="pyrH"
                     /locus_tag="ACFB2Q_002136"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC0919071.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
BASE COUNT          243 a          261 c          305 g          326 t
ORIGIN      
        1 agtggattaa atttaaaccg gtacggcaag gcgaggcaac gccgtaccgg tttaaattta
       61 atccactata atctccatat aataaatggc tgtctgaagc ccatatgcga ctatatctgc
      121 cgaccctatg gtcaatcatc aatcagcagt ggaccagtgt gccttcgtct tcgccggtaa
      181 tgacgcgttt gagcgagcct tgtttggcga tgccgaagac gacgatattg agcttgcgtt
      241 cgcggcagag ggcaaaggcg gtcgcgtcca tgactttgag gtttttattc aaggcttcgt
      301 caaaagtaat ggtttcgtag cgtgtggcgg acgggtcttt tttcgggtct gcggtgtaca
      361 cgccgtcgac gttggtggct ttgagcatca cgtcgcagtt catttccgcg ccgcgcaatg
      421 cggcggcagt gtcggtcgtg aagaacggat taccggtacc ggcggcgaaa atcacgactt
      481 tgccttcttc caaatattga atggctttag gacgggcgta agtttcagcg atttgctgca
      541 tagacagtgc ggattgtacg cgcgctttga tgcccaaggt ttcaaatgcg tctttgagtg
      601 ccaaggcgtt catcacggtc gccatcatgc ccatgtagtc ggcagtggcg cggtccatgc
      661 tgcctgcttg tgcagatacg ccgcggaaaa tgttaccgcc gccgacaacg ataccgactt
      721 gcacacccat tttcacgatt tcggcaattt cgccgacagt ttgaacaatg gtatcgtgat
      781 tgatgccgaa cggatcggaa cccatcaggg attcgccgga gagtttcagt aatacgcgtt
      841 tgtatttgat ttgctgtgtc atgggatacc ttgctttctt gaatgtcgtc ggtcggagtt
      901 gggacgtctg aatttcaaat ggtcggatta caatgatatg gtgtgtcgtt ctattattgt
      961 tttgttttca gacggccttt atattcggtc gtcttaaaat aggtaccgta aacggttttc
     1021 tcaaaaaaaa gcaccctgat tcgtttggaa tccaggtgct ttctttttca taagtgcctt
     1081 acactttggc ggctgcggct acttcggctg cgtagtcgac aacggctttc tcgat
//
LOCUS       DAXDSD010000190         1122 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXDSD010000190 DAXDSD010000000
VERSION     DAXDSD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.190"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..345
                     /locus_tag="ACFB2Q_002137"
     CDS             <1..345
                     /locus_tag="ACFB2Q_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC0919072.1"
                     /translation="VKGSQAETEGVWVHNDCPYGNLSDNKSVGEGKKFTPAQKKAIIQ
                     ENMNRNGGVVKSDQSGEVLVRPKKSQKGITPPPSNEWQIDHVQAKSKGGSNSYKNAQV
                     LSRRENIKKSNK"
     gene            354..626
                     /locus_tag="ACFB2Q_002138"
     CDS             354..626
                     /locus_tag="ACFB2Q_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919073.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIHEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            793..1023
                     /locus_tag="ACFB2Q_002139"
     CDS             793..1023
                     /locus_tag="ACFB2Q_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC0919074.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDATLEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
BASE COUNT          417 a          171 c          218 g          316 t
ORIGIN      
        1 gtcaagggca gtcaggcgga aacggaaggg gtttgggttc ataatgattg tccgtatgga
       61 aatttatcgg ataataaatc tgttggcgaa ggaaaaaaat ttacaccagc tcaaaagaaa
      121 gcaatcatcc aagaaaacat gaatagaaac ggaggggtag tgaagtctga ccaatctgga
      181 gaagttttgg ttcgtcctaa aaagagccaa aaaggtatca cccccccccc atcaaatgaa
      241 tggcagattg accatgtgca agcaaaaagt aaaggaggtt caaatagtta taaaaatgca
      301 caggttttat ccagaagaga aaatattaag aaatcaaata aataggtatc aatatgcaat
      361 ggaaatttga agatcctcaa aatactgctg ttattaccac cagaagaatt ttgtctaaaa
      421 aaaacggaat cttagaagta tggcatgatg ctgatgatgg aatgtggcaa ttcttagatg
      481 gcaatgaaat tcatgaagga gatgctgtga tagttagttt aaaagaaatt gtcactattg
      541 attcctcagt caatctctta agtaatttac ctcttggtgg ttacgcttgg agaaatacta
      601 tatcggaaaa atggaatttt gattagcaag cgtaggttag gttaaaaaaa ccaacaatca
      661 aaggtcgtct gaaacagtgt ttagtttttc aaacgacctt ttcttcaatt aacatagaat
      721 gttgggtctc gacccaacct acacgcttac taatctcaaa tataacctta tgaaataatg
      781 agtatttaaa agatggaaac attagacgaa cgaataaaga atttaggaaa atctcttgaa
      841 gatagaattg acgctaatct aattgatgcc acactagagt acattacttt ttcagaacgt
      901 ttgctagctt ttgaaacgct atgtgattat attgaagatt tcaatatcca gcttacggaa
      961 aaagaatccc aagaaatctc ttttattaat aaggaatttg gaattgaaag tacatcagat
     1021 taataaatat taggtcggtt taaaaaccca acaatcacaa ggtcttctga aaccgtgttt
     1081 aattttcaga cggcatttcc ttcatttgaa acaggatatt ga
//
LOCUS       DAXDSD010000191         1119 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXDSD010000191 DAXDSD010000000
VERSION     DAXDSD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.191"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>1119
                     /locus_tag="ACFB2Q_002140"
     CDS             <1..>1119
                     /locus_tag="ACFB2Q_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC0919075.1"
                     /translation="KGNLLGVPLRRGVSDSAFIDQITFSIHEDTFFHVYGLSFDLFDD
                     DDFIRAASAKMEEIFGFGIIEKAKHSGGRFYEGCWLMGTENAQYGRVHYGGQRETMLV
                     ELTAVGCNAANIGWESRLFDFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRNKGLF
                     TCHHVKPKGECLGSDWEEEDDETKMTSGKTYGIGSRESSKYVRIYEKGKQLGDKTSTW
                     TRFEIEFKAKDIVIPFEVLQTPGEYFGGAYPICERFTGSANRIEAVKSKIMIDFDTYI
                     ERLKKQIGRGINASKAVFPDKSKQELFEVLEPKHDFLPKKLSFENYDCSEAKLTPLHE
                     IPSVLKFDQYGMWMDRYIQRQKTGEEQRYLEKMYDKYAN"
BASE COUNT          350 a          218 c          280 g          271 t
ORIGIN      
        1 aaaggaaacc tgttaggcgt tcctcttagg cgcggtgtat cagattcggc tttcattgac
       61 caaatcacat tttcaattca tgaagacacg tttttccatg tttatggact ttcgtttgat
      121 ttgttcgatg atgacgattt catacgcgcg gcttccgcca agatggagga aattttcgga
      181 ttcggaatca ttgaaaaagc caagcattcg ggcgggcgtt tctatgaagg ctgctggctg
      241 atgggaacgg aaaatgccca atacgggcgc gtccattacg gcggccaacg tgaaacgatg
      301 ttggttgaat tgacggcggt aggctgcaat gctgcaaata ttggatggga atcaaggctt
      361 tttgattttc tgacaaatgc gattcgtcca aaaataacgc gcgttgacat tgcaaaagac
      421 tttttcaacg gagagtacag cccgaatcag gcaagagaag accgcaataa aggtttgttt
      481 acgtgccatc acgtcaaacc gaagggcgag tgtcttggtt ctgattggga ggaagaagac
      541 gacgaaacca aaatgacgag cggcaaaacc tacggcatag gttcgcgtga gtcttcaaaa
      601 tatgtacgaa tctatgaaaa aggcaagcag cttggcgata aaacaagcac atggacgcgc
      661 tttgaaatcg aattcaaagc gaaagacatt gtaattccct ttgaagtctt acagacaccg
      721 ggcgagtatt tcggcggagc atacccgatt tgcgaacggt ttacaggcag tgcaaaccgt
      781 attgaagcgg tcaaaagcaa gatcatgatt gatttcgata cctatataga acgactgaaa
      841 aagcaaatcg gacgaggaat caatgcatcg aaagcagtgt ttccggacaa atccaaacaa
      901 gaattgtttg aagtgcttga gccgaaacat gattttttgc ctaaaaaact gagcttcgag
      961 aattacgatt gcagcgaggc aaagctaacg ccactgcatg aaattccgtc cgttctaaaa
     1021 tttgaccaat acggcatgtg gatggatcga tatattcagc gtcaaaaaac aggggaagag
     1081 cagcgttatt tagagaagat gtatgacaaa tacgcaaat
//
LOCUS       DAXDSD010000192         1097 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXDSD010000192 DAXDSD010000000
VERSION     DAXDSD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.192"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(246..656)
                     /locus_tag="ACFB2Q_002141"
     CDS             complement(246..656)
                     /locus_tag="ACFB2Q_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919076.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTNFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(660..>1097)
                     /locus_tag="ACFB2Q_002142"
     CDS             complement(660..>1097)
                     /locus_tag="ACFB2Q_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919077.1"
                     /translation="GNKPAKQATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAIT
                     DGKYITDPLSMVRHKPDASSLDKIDSKSLFKSDVDAEKATLDAARYADKHNLWDSQNK
                     AKVPTNDVVGYTDGKPTHIINIYRRKPNKDGIRPVHGTPGKER"
BASE COUNT          331 a          193 c          168 g          405 t
ORIGIN      
        1 tgctgaacaa tgttttgtag aacttgctca aaatccatgt ttcgccattt tcctttatat
       61 atacctaatg cagcacgggt tacgatttcg tttaaatcat aaatagccat ttacgtcctc
      121 tgctaatttt cagcgagtag attgggttac caaacccaac aaaaaagcag cctgaaaaat
      181 ttcaggatgc ttgttttatg ttttttatca atgatttagg ttttaatcca gcctgcgctt
      241 gctagttaaa cagtatttac ataaaaatct tgcactgaac aaacaagcgt atcatgttca
      301 tcatgaaatg atgggcataa cgttaatcta caaatgttta catttaaaga tattttttca
      361 gtttcaatat ttttaatttg taaggttaat acatgaggat atttgtgata tagctgattt
      421 tttaattgtt cgttgtctaa tatagcaaaa ggtaattttg cacacaaata accaataact
      481 ttgtttttat gaaaaaaaac tatccaattt tcaattcctt catcccaatc aaaagtaaaa
      541 ttcgttcctt ctatgataaa tttagcacaa gatattaaat ctaacttaac ccaagtggat
      601 ttatacagac tcaatgctga tgaagttata atattatcta aattataaat tttcataaat
      661 caacgttcct tacctggtgt tccatgcaca ggtctaatcc catctttatt cggctttcta
      721 cgataaatgt taattatatg tgtcggtttt ccgtctgtat agccaactac gtcgttcgtt
      781 ggaacttttg ccttattttg actatcccat aaattatgtt tatctgcata tctagcagca
      841 tctaatgtag ctttttccgc atcaacatct gatttaaata aacttttgct atctatcttg
      901 tctagactag atgcatcagg tttgtgtcgt accatactga gagggtcagt aatatatttg
      961 ccatctgtaa tagctgatcc accattatag gtagtatgcg tataattttt ttgtggaacg
     1021 agactatttt gcaaagattg ttgtgtagaa acgccagctt tgccattagg agtagcttgc
     1081 ttcgctggct tattccc
//
LOCUS       DAXDSD010000193         1076 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXDSD010000193 DAXDSD010000000
VERSION     DAXDSD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.197"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..250
                     /gene="exp2"
                     /locus_tag="ACFB2Q_002143"
     CDS             <1..250
                     /gene="exp2"
                     /locus_tag="ACFB2Q_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein Exp2"
                     /protein_id="HFC0919078.1"
                     /translation="TLNLFVEGFWIIGLLQALAFTTIVDYCTESKHTNMLNNPACNDE
                     DWDNDMLNNPACNDEDWDNDMLNNPAYSWSMFNIHHSD"
     gene            454..1041
                     /locus_tag="ACFB2Q_002144"
     CDS             454..1041
                     /locus_tag="ACFB2Q_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFC0919079.1"
                     /translation="MIGFVKWYSYEKDFGFIFGEDGHEYFFRKSAGSDIPGQIVNFEP
                     FEHSRGLRARKVSNIHNPDPQQIQKLKTLCKDFQKVEKQPMSKKYNLLSAAILSLCLT
                     MFITLGFFYILAGFFDTDRETNIVLLFVFPAVTFSVLTFFLNRIFRNMKPGSDTDVAT
                     NDDWLKDFWSEDSLIYDPAYSNLNCNIFHRSDDDI"
BASE COUNT          338 a          195 c          216 g          327 t
ORIGIN      
        1 gacattaaat ctttttgttg aggggttttg gataattgga ttacttcaag cactcgcttt
       61 taccaccatt gtagactatt gcactgaatc taaacataca aatatgctca ataatccagc
      121 gtgcaacgat gaagattggg ataatgatat gctcaataat ccagcgtgca acgatgaaga
      181 ttgggataat gatatgctca ataatccagc gtactcttgg tcaatgttca atattcacca
      241 ttcagattaa aaaagcagga gccttatact cctgctttcc gcagcaaagt tacggatgta
      301 caacccggta aaaaaatctc cacttcctat gggcctgcct tggtcttaga aaaaggtgag
      361 aatggcttaa ttaaaattca gccaagcaga ggcaatgaac gttggattac aaatgatagc
      421 attcaagtaa cctataaata aaggagaagt atcatgatcg gattcgtaaa atggtatagc
      481 tatgagaaag attttggctt tatctttggt gaagatggtc atgaatactt tttccgtaaa
      541 tccgccggtt cagatatacc aggacagata gtgaatttcg aaccatttga gcatagtcgt
      601 gggctgcgag cgcgaaaggt tagtaatata cataatcctg atcctcaaca gatccaaaaa
      661 ttgaaaacct tatgcaaaga ctttcagaaa gttgaaaagc aacccatgtc taaaaaatac
      721 aacttgcttt cggcagctat attatcgctg tgtttgacta tgttcataac actgggcttt
      781 ttctacatct tagccggctt ttttgatact gatagggaga ctaacatagt tttgctgttt
      841 gtcttccctg cggtaacttt ttccgtgctg acattctttc tgaataggat attcaggaat
      901 atgaaaccgg ggtctgacac tgatgttgcc actaatgatg attggttaaa ggatttctgg
      961 tctgaggatt cattaattta tgacccagct tattccaatc tcaattgcaa tatatttcat
     1021 cgatcagatg acgatatata agaaagagga ttggtagctg aaatgctgcc aatcat
//
LOCUS       DAXDSD010000194         1063 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXDSD010000194 DAXDSD010000000
VERSION     DAXDSD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.198"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..396)
                     /gene="cas1"
                     /locus_tag="ACFB2Q_002145"
     CDS             complement(<1..396)
                     /gene="cas1"
                     /locus_tag="ACFB2Q_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC0919080.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQERKKVAQLPVHSIGHI
                     FCFGNVLVSPFLLGFCGENNVNLAFFTENGCYLGRLQGRQNGNVLLRRAQYRVSEQSP
                     MPIACNIIAAKIRAIRKRARACALSGQCMF"
     gene            complement(456..>1063)
                     /locus_tag="ACFB2Q_002146"
     CDS             complement(456..>1063)
                     /locus_tag="ACFB2Q_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919081.1"
                     /translation="FKNANFYGNTQIVNGDVHNVHTQNTPNSINENEIATYTPEPIWR
                     SPITMAWLTWASFLISLAGLLPIYNCIVKPILYLVNGKTMIQSEINNTYIFILIAIAV
                     LFLITITLRRITKYQTRYPLVFNYAISGMGHRLSLEKISISQCPQCGGEMKYYNKPIE
                     WENIRYSDGSKKRRVTRTTPALECKRNREHWYKVDPAEDKI"
BASE COUNT          306 a          244 c          185 g          328 t
ORIGIN      
        1 aaacatgcac tgtccactca aagcgcacgc ccttgcacgt ttccggatcg cccgaatctt
       61 tgccgcaatg atattgcacg caatcggcat gggactttgc tccgacaccc gatactgcgc
      121 ccgacgcaac agcacattgc cgttctgccg cccctgaagc cgtcccaaat aacacccgtt
      181 ttcggtaaaa aacgccaaat tcacattatt ttcgccgcaa aaccccaaca gaaacggcga
      241 cacaagcaca ttcccaaaac agaaaatatg cccgatggaa tgcaccggta actgcgccac
      301 cttcttacgc tcctgctcca ccaccagtgt ctcccgctcc ttatgcagat agctgccttg
      361 ggtggtgatg tagagtgtgt tttgcagttt gcgcatggga aaactcctta ggttttgacc
      421 gtgcttttgg ggttaaacga ggggctagct aaaaattaga ttttatcttc tgccggatca
      481 actttatacc aatgctcacg atttctttta cactctaatg ctggtgtagt tcttgttacc
      541 cttcttttct tgctaccatc agaataacgt atattctccc actcgattgg cttattataa
      601 tatttcattt ctccaccgca ctgtggacat tgagaaatac taattttctc tagacttaaa
      661 cgatgcccca taccgctaat agcataatta aaaactaatg ggtatctagt ttgatactta
      721 gtaattctgc gtaatgtaat agtgataaga aataatacag caatagctat taaaataaat
      781 atataagtat tatttatttc actttgtatc atagttttac catttactaa atataatatg
      841 ggtttaacta tacaattata aataggtaat agccctgcta aagaaatcag aaaactagcc
      901 catgtcagcc aagccatcgt aatggggctt ctccaaattg gttccggggt gtatgtggct
      961 atttcgtttt catttattga atttggtgta ttttgagtat gtacattatg aacatcacca
     1021 ttaactattt gagtattccc ataaaaatta gcatttttaa aat
//
LOCUS       DAXDSD010000195         1009 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXDSD010000195 DAXDSD010000000
VERSION     DAXDSD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.199"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            190..666
                     /locus_tag="ACFB2Q_002147"
     CDS             190..666
                     /locus_tag="ACFB2Q_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919082.1"
                     /translation="MEANKFEVKSLSDLIKVFAGIAADFEAAMGVKRADISTEFDEPQ
                     HEPQPPVTVAEQKGINDFAIGKEVIIRTYSAGVWFGVLEQKAGNEVILTKARRMYSWW
                     AKESISLSGVARHGIRQDGSQICGELDSVWLEAIEIIPVTGGAAESIRTALEVAQS"
     gene            663..914
                     /locus_tag="ACFB2Q_002148"
     CDS             663..914
                     /locus_tag="ACFB2Q_002148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919083.1"
                     /translation="MSYLDQPLKHGYGNGNGSGNGSGYGNGYGKGYGSGYGNGKGDGS
                     GSGNGDGDGNGNGSGNGSGNGDGDGNGNGSGNGSGYGNG"
BASE COUNT          304 a          247 c          293 g          165 t
ORIGIN      
        1 aaaggacggg caaagccttt tattgaaagt tggtgaaatc tgccgcgacg cagcagcgac
       61 atggaccact agaaaaagcg aaagcatcaa tcacaccgcc ttcactttta cagtgaaaaa
      121 agacggctta aaagagaagg taatgattgt tttgtaaaga aaccaacccc accccgaaag
      181 gaaacaaaaa tggaagcaaa taaatttgaa gtgaaaagtt tgtcagacct tataaaagtc
      241 tttgcaggca ttgctgccga tttcgaagcg gcaatgggtg taaaacgcgc cgacatttca
      301 accgaatttg acgaaccgca acatgagccg caaccgccgg taacagttgc cgagcaaaaa
      361 ggtatcaacg actttgccat cggcaaggaa gtcatcatcc gcacttattc ggcaggcgtt
      421 tggtttggtg tgttggaaca aaaagcaggc aatgaagtga ttctgacaaa agcgcgccga
      481 atgtacagct ggtgggcaaa ggaatcaatc agcctgtcag gtgtcgcacg acacggcatc
      541 aggcaagacg gcagccaaat ttgcggcgag cttgattccg tatggctcga ggcgattgag
      601 attatcccag taacaggcgg cgcggctgaa tcaatccgca ccgcgctgga ggtcgcccag
      661 tcatgagtta tctagatcaa ccattgaagc acggctacgg caacggcaac ggtagcggca
      721 acggtagcgg ctatggcaac ggctacggca agggctacgg cagcggctac ggcaacggca
      781 agggcgacgg cagcggcagc ggcaacggcg acggcgacgg caacggcaac ggtagcggca
      841 acggtagcgg caacggcgac ggcgacggca acggcaacgg tagcggcaac ggtagcggct
      901 atggcaacgg ctaaacctta aaaccgcccg aagctatatc tgccaagccg gggatagcac
      961 aaagcgatga agtatcgaac ttcaaagcgg ggcaacggca cggcgaaac
//
LOCUS       DAXDSD010000196          976 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXDSD010000196 DAXDSD010000000
VERSION     DAXDSD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.200"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..158)
                     /locus_tag="ACFB2Q_002149"
     CDS             complement(<1..158)
                     /locus_tag="ACFB2Q_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC0919084.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGM"
     gene            complement(294..>976)
                     /locus_tag="ACFB2Q_002150"
     CDS             complement(294..>976)
                     /locus_tag="ACFB2Q_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC0919085.1"
                     /translation="PNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEII
                     NLDFADVKTVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNI
                     TTAPGCLKMSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGA
                     VDPTPAREVEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAIL
                     RRQHNSDK"
BASE COUNT          238 a          261 c          234 g          243 t
ORIGIN      
        1 tccattccgg tctcgcccaa aatcagggga ttgacgataa cgatgtagca catcgtcaaa
       61 aaggtcgtca aacccgccat caactcggta cgtaccgtcg taccgtttgc cttcaggtta
      121 aaaaccctat ccagcaatgt ttgttttgaa atatccatat ctagcagtcc gtttcaagta
      181 aaaaatctga acccgcacac cgcaaaccgc acaggattcg gcatattgaa aaatatacct
      241 cccaaatcgg gaaaaccggt tcgggaggca ataagtttac ggcagacggc acattatttg
      301 tctgaattgt gttgacgacg caaaattgca ggaatttcaa aatcgtcaag gacagactgg
      361 ttatcgaaat ccgctgctgt cagattcatg gtgcggatac cgcgattggt gcggatcata
      421 ccttcgacat tgtggctttg ctcctgtttg gacggggcaa ccgcttctac ttcccttgcc
      481 ggagtaggat cgaccgctcc tttttctttc agaccggtag cgataatggt aatccggatg
      541 gcatcttcgc tcatggtctc gtcttcagcg gcaccgaatt tgcattccaa atcgggatgc
      601 gcgctttggt tgacgatttt catgacttca gacaactcgg acattttcag gcaacccgga
      661 gcagtcgtaa tattgaccag cacaccgcgt gcaccatcca aagttacatc gtccagcagc
      721 ggactggaaa tggcctggtc ggtcgccata cgcgcacggt cgataccttg ggcataaccc
      781 gaacccatca tagcgatacc gcggttgctc atcacggttt tcacgtcggc aaagtcgagg
      841 ttgatgattt cgctcgggca agttaccact tcggaaatgc ctgcgaccgc atcgcgcaat
      901 acattgtccg ccgcgcggaa ggcttcgcgc atagttacgt cttcacccaa tgcggtcatc
      961 agtttgtcgt tcggga
//
LOCUS       DAXDSD010000197          932 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXDSD010000197 DAXDSD010000000
VERSION     DAXDSD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.201"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>932
                     /gene="tuf"
                     /locus_tag="ACFB2Q_002151"
     CDS             <1..>932
                     /gene="tuf"
                     /locus_tag="ACFB2Q_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC0919086.1"
                     /translation="KKFGGAAKAYDQIDNAPEEKARGITINTSHVEYETETRHYAHVD
                     CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFMNKCD
                     MVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALKALEGDAAYEEKIFELATALD
                     SYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTT
                     CTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLS
                     KEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEKGV"
BASE COUNT          250 a          270 c          233 g          179 t
ORIGIN      
        1 aaaaaattcg gcggcgctgc aaaagcttac gaccaaatcg acaacgcacc cgaagaaaaa
       61 gcacgcggta ttaccattaa cacctcgcac gtagaatacg aaaccgaaac ccgccactac
      121 gcacacgtag actgtccggg tcacgccgac tacgttaaaa acatgattac cggcgccgca
      181 caaatggacg gtgcaatcct ggtatgttct gctgccgacg gccctatgcc gcaaacccgc
      241 gaacacatcc tgctggcccg tcaagtaggc gtaccttaca tcatcgtgtt catgaacaaa
      301 tgcgacatgg tcgacgatgc cgagctgttg gaactggttg aaatggaaat ccgcgacctg
      361 ctgtccagct acgacttccc cggcgacgac tgcccgatcg tacaaggttc cgcactgaaa
      421 gccttggaag gcgatgccgc ttacgaagaa aaaatcttcg aactggctac cgcattggac
      481 agctacatcc cgactcccga gcgtgccgtg gacaaaccat tcctgctgcc tatcgaagac
      541 gtgttctcca tttccggccg cggtaccgta gtcaccggcc gtgtagagcg aggtatcatc
      601 cacgttggtg acgagattga aatcgtcggt ctgaaagaaa cccaaaaaac cacctgtacc
      661 ggcgttgaaa tgttccgcaa actgctggac gaaggtcagg cgggcgacaa cgtaggcgta
      721 ttgctgcgcg gtaccaaacg tgaagacgta gaacgcggtc aggtattggc caaaccgggt
      781 actatcactc ctcacaccaa gttcaaagca gaagtgtacg tattgagcaa agaagagggc
      841 ggccgccata ccccgttttt cgccaactac cgtccccaat tctacttccg taccactgac
      901 gtaaccggcg cggttacttt ggaaaaaggt gt
//
LOCUS       DAXDSD010000198          921 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXDSD010000198 DAXDSD010000000
VERSION     DAXDSD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.202"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..551
                     /gene="mafB"
                     /locus_tag="ACFB2Q_002152"
     CDS             <1..551
                     /gene="mafB"
                     /locus_tag="ACFB2Q_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC0919087.1"
                     /translation="KWDWVKNTGYETTVTRKTLDGQIAGKNKPIKPSSGDYVLPVGRQ
                     IIDPTRTSFSQATVSYQKRGANYNYDSLVAAMNEKKSWVGDRVDVVNMPDGAPTSMDN
                     TRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQTWGEAIQLRIRKQETQKG
                     VPEGWSKRFPNGSIYDVKVLRK"
     gene            548..919
                     /locus_tag="ACFB2Q_002153"
     CDS             548..919
                     /locus_tag="ACFB2Q_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC0919088.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          326 a          146 c          190 g          259 t
ORIGIN      
        1 ccaaatggga ttgggttaaa aataccggct atgaaacaac tgttacccga aagactttag
       61 atgggcaaat agctggcaag aacaagccta ttaaaccttc ttcaggagat tatgtcttgc
      121 cggtaggcag acaaatcata gatcctactc gtacatcttt ttcacaagct acagttagct
      181 atcagaagcg tggtgcaaat tacaactatg attctcttgt agctgcaatg aacgaaaaaa
      241 aatcatgggt tggcgacagg gttgatgttg ttaatatgcc tgatggagca cctactagta
      301 tggataacac gcgtattatg gcagcacgtg aagcaggggt aaaagtggaa gcgaatgttc
      361 ataattttaa tgaccgatta tcatcaaaag agagaatcag gtttaagcat gatggtattg
      421 agcctcaaac ttggggagaa gctatccagc tacgaattag aaagcaagaa acacaaaaag
      481 gagttccaga agggtggagc aaaagatttc ctaacggaag tatttatgat gtaaaggtac
      541 ttaggaaatg ataaaacaaa atagttttgt tccgtatcct gaagcaatgc ttcctaaagg
      601 atttaaatat ccgcaaagtt atttaaaatt agctcaatcc actcatgcca ttaactacga
      661 tgaacaatat tcttttcctt ggtggtttga aaatgcagaa agcaatatat cagaagtaat
      721 tgacatttat tttgaaataa ctggcattcc aaacctatta ccttttgcta gaaaccaaga
      781 gtgggctgcc tgttttgata tttcagataa atcaggtaat cctaaaatta tagtagttaa
      841 tttagataat acaaaatatt acgagacttt tgaaaatttt gatacttggc taaaagaagc
      901 tgaaaatgat ggttggtagc a
//
LOCUS       DAXDSD010000199          920 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXDSD010000199 DAXDSD010000000
VERSION     DAXDSD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.203"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..390
                     /locus_tag="ACFB2Q_002154"
     CDS             <1..390
                     /locus_tag="ACFB2Q_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC0919089.1"
                     /translation="WHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            387..806
                     /locus_tag="ACFB2Q_002155"
     CDS             387..806
                     /locus_tag="ACFB2Q_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFC0919090.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
BASE COUNT          327 a          148 c          188 g          257 t
ORIGIN      
        1 tggcatacct actttgtcaa aggcgataag gcggaaacgg aaggggtttg ggttcataat
       61 gattgtccgt atggcggttc aaataattta gagaaagcaa aacttcgcgc agaacggctt
      121 agtaaaaacg atcgggcagg aaaagacttt actaaagcag gaaaagaagc cgttattgat
      181 ttgaatagga ttcaaaataa tggtcaagtt aagtgcgcta attgcggtat tgaaacaatt
      241 ccagccaaac aatctataaa aaatatttct cctacttcaa atgaaagaca agtagatcat
      301 gtcatcccta aatctaaggg tggtcaaggt acacctaaaa acgggcaggt attatgcaga
      361 ggctgcaaca ttaagaagag taacaaatga aatcgaaact tactgtcgtc tattacgatt
      421 tggaaagcaa tattgcagaa gaaatactgt caggaaacat aatgcctgat ggaaattttc
      481 ttattcaaga gattcctctt ttcgcaccga atttggcttt aaacgacatt gttgccatag
      541 aacgtgagga taagatgctg tttttcgacc acttgataaa agcttcagga aataccacga
      601 taaacatcgt tgttttggat catttcccaa aggatttatt ggcagccata gaagaacaca
      661 gtggtaaaat cagaaaaaat ggagagaatt atttatcggt taattttccg cccaaaaaat
      721 ataattctga tttaaaagga attttaaata gatatgagga agcaaatatc ctcagctaca
      781 gggaagcttg tttgggcttc tcttaatcga tctataagta agaatggatt acgttagatt
      841 gtaaacctaa cgattaaaag gtcgtctgaa accgtgttta attttcagac gacctatttc
      901 cttcatttga aacaggatat
//
LOCUS       DAXDSD010000200          902 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXDSD010000200 DAXDSD010000000
VERSION     DAXDSD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.204"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..185
                     /locus_tag="ACFB2Q_002156"
     CDS             <1..185
                     /locus_tag="ACFB2Q_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919091.1"
                     /translation="PLKQLGCKQAVTMWTVWHETRPILKRSAAALEVLRGYADKDASG
                     RIAACLEGIYGKAAAR"
     gene            complement(386..766)
                     /locus_tag="ACFB2Q_002157"
     CDS             complement(386..766)
                     /locus_tag="ACFB2Q_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC0919092.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
BASE COUNT          198 a          229 c          247 g          228 t
ORIGIN      
        1 tgccgctgaa gcagctcggc tgcaaacagg cggttacgat gtggacggtt tggcacgaaa
       61 cccgtccaat cctgaaaaga tccgccgcgg ccctcgaagt gttgcgggga tatgcggaca
      121 aagacgcctc cggccgcata gccgcatgtc ttgaaggcat ttacggcaag gctgcggcaa
      181 ggtaaacgga aaatgccgcc tgaaggttcg gacggcattt ttattgaagg gatgattggg
      241 gatttgtagg cgtttgcaga tacgacaagg ggcgcattcc gcgccccgtc acacgcacgg
      301 acggcttgtc atcgcctgtc gcccgtgcgc ggctgcccat atttcggaac agtcttgcaa
      361 gcccctttaa gggaacggtt ttattctagt acagtttgaa tgccttggca acggctgttt
      421 taaccgcctg aaaatcctct tcactgatta ttgggatgca acggtcgcgc cctttgggtt
      481 tgtatcggtc taatcgtgcc aatccgactg ttgccgtcat atcgcatttt gcccgacatt
      541 ggatgtgcgg cttgtccggt aaggggtttt cactcatttt gtggtggcag tccgccaaag
      601 ggacaggctc tgtgctgctt aagggtacga ccgttaccag tttgccgttg tgcctgtttc
      661 gcgctatgac gacgacaggg cgtttcttga ccatttccgg ttcttcataa ccgcgaaagt
      721 cgcacatgat aactgaacgt tcccttggtt ggaattttaa aggcattagc cgctcctgac
      781 gatgacaggc ggctattata gcagttctta cacaaaaaac cgctttgtgt aaggcggttg
      841 caaaaaaaag ccttccaata aaaatgccgc ctgaaccttt cagagtgcga agcctgccca
      901 ta
//
LOCUS       DAXDSD010000201          882 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXDSD010000201 DAXDSD010000000
VERSION     DAXDSD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.205"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..204
                     /locus_tag="ACFB2Q_002158"
     CDS             <1..204
                     /locus_tag="ACFB2Q_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HFC0919093.1"
                     /translation="MENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETNI
                     DGGPNEMTLAEAKEAYAKGLPIR"
     gene            225..584
                     /locus_tag="ACFB2Q_002159"
     CDS             225..584
                     /locus_tag="ACFB2Q_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919094.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLDRI
                     TTNECINGIKKSIKKTN"
BASE COUNT          337 a          125 c          159 g          261 t
ORIGIN      
        1 atggagaatt caaaaaagac atgggaaatt gacggagaaa tatggctaca ttgtcctgtt
       61 tgcggaactg aagttatgga ctatgatatc tgtgacgttt gtcagtggca aaatacagga
      121 gaaactaata tagatggtgg tcctaatgaa atgacacttg cggaggcgaa agaagcttac
      181 gcaaaaggct taccaatcag ataaataagc acctagagaa accaatgatg acggaacccc
      241 atggttaccc atcaaataat ttacctgtta aaatcataaa tgatattatt tatgcaaccc
      301 agttagtcga agatttggtt ttaggaaaaa taaaaattgt tgatttttta aaatcatata
      361 acaattttta ttattggctt ggttttgatg agttacctca atctgagaaa ataaaatttc
      421 taaactatct caatatatta agtattcata aggaaataca agataaaact gtgaataggg
      481 tttataccga ttgttttgat attgataaat tacattcgct tgacagaatt acaactaatg
      541 aatgtattaa tggtataaaa aaatctatca aaaaaacaaa ttagaatttg acaatatact
      601 caaataacta gcctatgttt tgtaaaataa aaatattctt gctgagtgta gagccagaga
      661 taaaaagatt tgatagtagt ttgcagttgt agaattatat aaacaagaaa atagtcagca
      721 gccgtaggtc ggattctcga atccgacatt ttccaacagc ggcatttcgg aaacgataga
      781 tgcgtcaaat atttttgtcg gatacaaata tccgacctac atctctgcgc agcaaacttt
      841 acaagatatt aatgaattag gaaatttaag tccggaagca ca
//
LOCUS       DAXDSD010000202          831 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXDSD010000202 DAXDSD010000000
VERSION     DAXDSD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.206"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..356)
                     /locus_tag="ACFB2Q_002160"
     CDS             complement(<1..356)
                     /locus_tag="ACFB2Q_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFC0919095.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKP"
     gene            complement(358..636)
                     /gene="secE"
                     /locus_tag="ACFB2Q_002161"
     CDS             complement(358..636)
                     /gene="secE"
                     /locus_tag="ACFB2Q_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC0919096.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(751..826)
                     /locus_tag="ACFB2Q_002162"
     tRNA            complement(751..826)
                     /locus_tag="ACFB2Q_002162"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(791..793),aa:Trp,seq:cca)
BASE COUNT          255 a          212 c          123 g          241 t
ORIGIN      
        1 ttcggcttct ctatgccggt ctgaacctgc tgtaaaataa tttcagcctc tctctgacta
       61 atcggcgtgg gtctattagc cctccctcca ataaaaccag aaacacgggg agtgcttttt
      121 acaagatgcc aagagtcatc tgtcatttcc atctcaacta acacataacc aggatatgac
      181 tttctttcac taatagtctt acgaccattg cggatatcaa caactttctc tacaggcacc
      241 agaatttgtc cgaaataatc tcccatctcc tcacgggcaa ttcgctcttc caatattcgt
      301 tggacattct tctcaaaccc cgaatacgcc tgtacaacat accatttttt cgacatctca
      361 accttccctt ctcagcaata catcaaaaaa taaccacgaa attgctgtat ctgccgcata
      421 gataaatata gaaagcacag caacaaacac tataacaaat acagtcattc tgacggcatc
      481 ttcacgctta ggccaaacca cctttttgaa ttcggaccaa gaatttgaga aatatgcaaa
      541 aaaaccctcc ttaccggaat tagatgcaga ttctttatct tgaacaacca gttgattcac
      601 tttaacattc tttttttcag gcgtatgttc tgtcatatta tttatccatc atagcatctg
      661 tctattctca atccatgtaa atggcaagag agtttactaa ataacaaata caaaaaaatt
      721 aaccggcaca aggccggtta attttttatt tggcaggcca agagggtctc gaacccccaa
      781 ccctcggttt tggagaccga tactctacca attgagctat tggcctctaa a
//
LOCUS       DAXDSD010000203          829 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXDSD010000203 DAXDSD010000000
VERSION     DAXDSD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.207"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..285
                     /locus_tag="ACFB2Q_002163"
     CDS             <1..285
                     /locus_tag="ACFB2Q_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC0919097.1"
                     /translation="GNKPVVKSIRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDG
                     VKIDIRPNGEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
     gene            282..599
                     /locus_tag="ACFB2Q_002164"
     CDS             282..599
                     /locus_tag="ACFB2Q_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919098.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            669..829
                     /locus_tag="ACFB2Q_002165"
                     /pseudo
     CDS             669..829
                     /locus_tag="ACFB2Q_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT          291 a          144 c          159 g          235 t
ORIGIN      
        1 ggaaacaagc cagttgttaa atctattaga ccaactacgc gagatgaatt acgtcaagca
       61 ttgcaagaac aaggttttag acgtactggt tcagatgcgg ctcaatatga aacatggaaa
      121 ggtcctgatg gcgtgaaaat agatattcgt ccaaatggag aggttataag aacccaaaga
      181 gtgccgcgaa ccgatggtgt acagggaaaa tatccgcaac gacaagatta tgaaggcaat
      241 ccattgccaa ataatcatca tcattctgga tattttgtca aatgaaaaaa aatatttttc
      301 acaatgtaag cctttatgaa ataatctttt ccgataatgg aaataccctt acattatctt
      361 ttacagatac aattgaaggt aattatttcg gatatatcaa atgcagtaat attttgaatt
      421 ttaaattaga tacaaataat ttcgtagatt atgaggataa ggaagatagc ttgtttccct
      481 tgtttatacc cgaaatagag ctatataaat accaatttta tagtgaaatt attattgatg
      541 tagggattat tataaaaata tctgctgaaa caattaattt tgagccactg ggaaaatagt
      601 aactgctttc ccagcagccg tagcaactgt atttttaccc gacggggtaa aaatacagtt
      661 gctacatctc tgcgcagcag actctacaag gtattaataa ttcaggaaaa ttaagtccgg
      721 aagcacaact tgccgccgtg agcctattac aggacagtgc ttttgcggta aaagacggta
      781 tcaactctgc cagacaatgg gctgatgccc atccgaacat tacagctac
//
LOCUS       DAXDSD010000204          815 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXDSD010000204 DAXDSD010000000
VERSION     DAXDSD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.208"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>815
                     /gene="mafA"
                     /locus_tag="ACFB2Q_002166"
     CDS             <1..>815
                     /gene="mafA"
                     /locus_tag="ACFB2Q_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC0919099.1"
                     /translation="RAAVKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIR
                     GGYHNNPDSATRYSYPAYDTTATTKSDALSGVTTSTSVLNAPAAALTKNNGRKGERSA
                     GLSVNGTGDYRNETLLANPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFG
                     TVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPY
                     SVGKTVKASDRLMVDFSDITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
BASE COUNT          212 a          311 c          174 g          118 t
ORIGIN      
        1 cgcgccgccg tcaaagaaat ggacttgtcc gccctgaaag gacgcaaagc cgccctttac
       61 gtctccgtta tgggcgacca aggttcgggc aacataagcg gcggacgcta ctccatcgac
      121 gcactgatac gcggcggcta ccacaacaac cccgacagcg ccacccgata cagctacccc
      181 gcctatgaca ctaccgccac caccaaatcc gacgcgctct ccggcgtaac cacttccaca
      241 tcggtcttaa acgcccccgc cgccgccctg acgaaaaaca acggacgcaa aggcgaacgc
      301 tccgccggac tgtccgtcaa cggcacgggc gactaccgca acgaaaccct gctcgccaac
      361 ccccgcgacg tttccttcct gaccaacctc atccaaaccg tcttctacct gcgcggcatc
      421 gaagtcgtac cgcccgaata cgccgacacc gacgtattcg taaccgtcga cgtattcggc
      481 accgtccgca gccgtaccga actgcacctc tacaacgccg aaacccttaa agcccaaacc
      541 aagctcgaat atttcgccgt cgaccgcgac agccggaaac tgctgattgc ccctaaaacc
      601 gccgcctacg aatcccaata ccaagaacaa tacgccctct ggatgggacc ttacagcgtc
      661 ggcaaaaccg tcaaagcctc agaccgcctg atggtcgatt tctccgacat caccccctac
      721 ggcgacacaa ccgcccaaaa ccgtcccgac ttcaaacaaa acaacggtaa aaaccccgat
      781 gtcggcaacg aagtcatccg ccgccgcaaa ggagg
//
LOCUS       DAXDSD010000205          798 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXDSD010000205 DAXDSD010000000
VERSION     DAXDSD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.209"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>798
                     /gene="pilC"
                     /locus_tag="ACFB2Q_002167"
     CDS             <1..>798
                     /gene="pilC"
                     /locus_tag="ACFB2Q_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC0919100.1"
                     /translation="NNTDTLVSQQSGTAVFGTATYLPPYGKVSGFDADALKERNNAVD
                     WIRTTRIALAGYSYADVICKSGNQCPQLVYETKFAFGQQGLQRKGNKLDIYEDKSRDN
                     SPIYKLKDYPWLGVSFNLGSENTVKNSKSFNKLISSFNEDNNNQTIVSTTRDHPISLG
                     NWQREHTAVAYYLNAKLHLLDKKRIEDIAQGKTVDLGTLRPRVETTGRGWLSFWATWD
                     IKDTGQIPVKLGLQVKAGRCINKPNPNNNTKAPSPALTAPALWFGPGQ"
BASE COUNT          230 a          223 c          183 g          162 t
ORIGIN      
        1 aacaataccg atacccttgt ttcccaacaa agcggtactg ccgtttttgg cacagccacc
       61 tacctgccgc cctacggcaa ggtttccggt tttgatgccg acgctctgaa agagcgcaac
      121 aatgccgtcg attggattcg taccacccgc atcgcgctgg caggctacag ctacgccgac
      181 gtcatatgca aaagcggcaa ccaatgcccc caacttgtct atgagaccaa atttgccttc
      241 ggtcaacaag ggttgcaaag aaagggcaac aagctggata tatacgaaga caaaagccgc
      301 gacaattcgc ccatttacaa attgaaggat tatccatggt tgggcgtgtc tttcaatttg
      361 ggcagcgaga ataccgtcaa aaatagcaaa tcattcaaca aattgatatc ttcttttaat
      421 gaagacaata ataatcaaac catcgtctct acgacacgag accaccctat ttcccttggc
      481 aactggcagc gcgaacatac cgccgtggcc tattatctga acgccaaact gcacctgctg
      541 gacaaaaaaa ggattgaaga tatcgcccaa ggcaaaacag tggatttggg caccttgaga
      601 ccgcgcgtcg agacgacagg acgaggctgg ctaagttttt gggctacgtg ggacattaaa
      661 gataccgggc agattccggt caagctcggc ctgcaagtca aagcaggccg ctgcatcaac
      721 aaaccgaacc ccaataataa taccaaagcc ccttcgccgg cactgaccgc ccccgcgctg
      781 tggttcggac ccgggcaa
//
LOCUS       DAXDSD010000206          777 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXDSD010000206 DAXDSD010000000
VERSION     DAXDSD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.210"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..183
                     /locus_tag="ACFB2Q_002168"
     CDS             <1..183
                     /locus_tag="ACFB2Q_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HFC0919101.1"
                     /translation="LRAQTLATIAAVCKLLNSGQTPPPDYGKRCKACSLAEICQPELL
                     GKRDRSVGYVKGLFGE"
     gene            299..658
                     /locus_tag="ACFB2Q_002169"
     CDS             299..658
                     /locus_tag="ACFB2Q_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919102.1"
                     /translation="MTRNIEHQFSNLSDVGEKLELENPTVENVVDILVDIGHDDRVYT
                     FHDDFLGLKSGLPQDLLSKHIDELEEGDFADRYSDEIDKILDNANIIFYHLERELSED
                     DLEEIREERERLGLEDD"
BASE COUNT          257 a          129 c          169 g          222 t
ORIGIN      
        1 ctgcgcgccc aaaccctcgc caccatcgcc gctgtgtgca aactcctaaa cagcggccaa
       61 accccgccgc ccgactacgg caaacgctgc aaagcctgct cgctggcgga gatttgccag
      121 ccggagttgc tgggaaaacg ggataggagt gtgggatatg tgaaggggtt gtttggggag
      181 taatatttta tttgaaaaaa attattacgt taattaactt gaatcttcca aattaatcgc
      241 aatttctgaa aacgagcaat catgctcaat tgtaattaca cattttaagg agtcgtaaat
      301 gacaagaaat atagaacatc aatttagcaa tctatctgat gttggtgaaa aactagagtt
      361 agaaaatcct actgtagaga atgtcgtaga tattttagtg gatattggac atgatgaccg
      421 agtttatact tttcatgatg actttctcgg gttaaaaagc ggtttgccac aagacttatt
      481 gtcgaaacat atcgacgagc ttgaagaagg tgattttgca gatagatatt cagatgaaat
      541 tgataaaata ctggataatg caaatataat attctatcac cttgaacgag agcttagtga
      601 agatgattta gaggaaatac gagaagaaag agaaagactg ggtttagaag acgattagta
      661 tacttgttgt catagaatag attcaataca tacaggttgg gttatgctgt tttacccaac
      721 cagctaacct cagggcatat ttaatattat ttaattctga ataatattct attgaag
//
LOCUS       DAXDSD010000207          770 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXDSD010000207 DAXDSD010000000
VERSION     DAXDSD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.211"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(99..422)
                     /locus_tag="ACFB2Q_002170"
     CDS             complement(99..422)
                     /locus_tag="ACFB2Q_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919103.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEHDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(425..613)
                     /locus_tag="ACFB2Q_002171"
     CDS             complement(425..613)
                     /locus_tag="ACFB2Q_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HFC0919104.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNINKVEI
                     HYIENTRTGEKTDFKFKD"
BASE COUNT          197 a          134 c          106 g          333 t
ORIGIN      
        1 tatttttatt tccagtcatt ctggctattt tatgtatatt tccattatgt ttgattatca
       61 ttttattttt tccaaggatt ttcttctata ttgatagttt atctaaagat ttcatttaaa
      121 atatcctgac taatttcttg tttatataat tctacaattt tctgtttctg tgtatttgta
      181 taaaattttt cgttggtatc tgcttgatat tttaattgtc tcaatatttc catgatttca
      241 tattctgatt tttcatcatg ttcttcaatc atgtaatcaa caaattgttt tagaatatca
      301 aaggcttcgt aactttccaa aattttaata tgtttatcgg caaaattgga catataccat
      361 tcgtcagtga ttggattggt atttaaatca tctaaaaaaa catttacatc atctaaaaac
      421 atggctaatc cttaaacttg aaatctgttt tctcaccggt tcttgtattt tcaatgtaat
      481 gtatctcaac tttgtttata ttttgaacgc gcttaaccca gccgtctttt gccaaccaac
      541 catttttagt atcggacata ggaggtattc tcgcaggagt tttgccctgt gggtttgcct
      601 taacttcttc cattgctaat tgttccttta aattattagg tattgtacga ccggttgatt
      661 tttcaacaga ttgtgtccct atttttaatg atttgtttcc ccccagccat ttccctgtct
      721 acagtctgca tatggcgggc agtaggtttt tcatagccgg tatctttaac
//
LOCUS       DAXDSD010000208          768 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXDSD010000208 DAXDSD010000000
VERSION     DAXDSD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.212"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            38..>768
                     /locus_tag="ACFB2Q_002172"
     CDS             38..>768
                     /locus_tag="ACFB2Q_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC0919105.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPAGKLVSLLGPSGCGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSAEAIYRKPENAFVTEFLGEADA"
BASE COUNT          204 a          255 c          167 g          142 t
ORIGIN      
        1 aagacaaaaa actcgccgcc gccgaaagga acgcagtatg agtattacca ttcaaaactt
       61 aaacaaaaaa cgcttcggca attttcacgc gctgaaaaac atcaacctca acgtccccgc
      121 cggcaaactc gtttccctgc tcggtccctc cggctgcggc aaaaccacac ttttacgcat
      181 tatcgccgga ctggaaaacg ccgacggcgg ccaaattctc tttgacggcc aagacgtaac
      241 cgccaagcat gtgcgcgagc gcaaagtcgg tttcgtgttc caacactacg ccctcttccg
      301 ccacatgaat gtgtttgaca acgtcgcttt cggtttgacc gtattgccca agcccgaacg
      361 cccgtccaaa ggacaaatcc gcgccaaagt cgaagaattg ctcaaactcg tacaactctc
      421 ccacctcgcc aaatcctatc cgcaccaact ctccggcggg caacgccagc gcatcgccct
      481 cgcccgcgcg cttgcggtcg aaccgaaact cctgcttttg gacgaaccct tcggcgcgct
      541 ggatgccaaa gtgcgcaaag aattacgcac ctggctgcgc gacattcatc acaacctcgg
      601 cgtaaccagc attctggtga ctcacgacca agaagaagcc ctcgaagttt ccgacgaaat
      661 cgtcgtgatg aaccatggca aaatcgaaca aaccggcagt gccgaagcca tttaccgcaa
      721 acccgaaaac gccttcgtta ccgagttcct cggcgaagcc gacgcatt
//
LOCUS       DAXDSD010000209          761 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXDSD010000209 DAXDSD010000000
VERSION     DAXDSD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.213"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..360
                     /locus_tag="ACFB2Q_002173"
     CDS             <1..360
                     /locus_tag="ACFB2Q_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC0919106.1"
                     /translation="NRAETEGVWVHNECPYGKGNQRYKDAPYHGKNDNSVKSRAPTNG
                     QAVLDNSVQVKSTSSQRVGVDKTNNEIVVLNQTRIFNDGSAEYHGHVRNWKNLHTDQQ
                     NALKKAGLVNSKGKIKK"
     gene            357..692
                     /locus_tag="ACFB2Q_002174"
     CDS             357..692
                     /locus_tag="ACFB2Q_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919107.1"
                     /translation="MMADKEQVEKMIYSENKDCVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
BASE COUNT          257 a          121 c          175 g          208 t
ORIGIN      
        1 aatcgggcgg aaacggaagg ggtttgggtt cataatgagt gtccgtatgg aaaaggcaac
       61 caacgatata aagatgctcc ttatcatggt aaaaatgata attctgtgaa aagtagagcg
      121 ccaacaaatg ggcaagctgt ccttgataac tctgttcaag ttaaatcgac atcttctcaa
      181 agagttggag ttgataaaac caataatgaa atcgttgtat taaaccaaac tagaattttt
      241 aatgacggtt ctgctgagta tcatgggcat gtaaggaact ggaaaaattt acatactgat
      301 caacaaaatg ctttaaagaa agcaggattg gttaattcaa aaggaaaaat caaaaaatga
      361 tggctgataa ggaacaagta gaaaagatga tttattctga aaataaagat tgtgttattg
      421 atggtatgct cggtatgacc ttcagctcgg aggaggatga aatcccttgg atttccgaga
      481 aattgacgga gttgtcgaag cataaagact tggatattgc cagattgtca ttaacctgtt
      541 tcggacatct cgccaggatg catgaaaata tcggcgattg cgataaagtg attgctttat
      601 tgcgctcaaa acaaggtaat ccggattttc aaggttttgc cgaagatgcg ttagacgata
      661 tttccttctt tatatttaaa aagcgtccgt aagcgcggat taaacccgga aatccgatgc
      721 gtctgaaagt acattccggc tttcagacgg catcggtaac a
//
LOCUS       DAXDSD010000210          755 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXDSD010000210 DAXDSD010000000
VERSION     DAXDSD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.214"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..555)
                     /locus_tag="ACFB2Q_002175"
     CDS             complement(<1..555)
                     /locus_tag="ACFB2Q_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC0919108.1"
                     /translation="MALRNASDFLGAYGGGVRVERRQCAEHGGYAAKSVLRGVWTGCP
                     ACRKLEAADEMAAYAETLRRGAMRDALEKRIGRSGIAPRFRNCRIENYAVSDSIPGMA
                     RAKAAAAEYAANFADVLQTGRSMIFSGRRGTGKNHLACGIAREVIAAGKSALVITVGD
                     MLRTVKDSFGGGGGEAGAVGIFVKP"
     gene            complement(567..>755)
                     /locus_tag="ACFB2Q_002176"
     CDS             complement(567..>755)
                     /locus_tag="ACFB2Q_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919109.1"
                     /translation="KCGMTALQAAEYAIASGWGSFRADWLQNKTFGRSGNRGGLTHNQ
                     TAAVPDAGSYGDMPTTDF"
BASE COUNT          113 a          293 c          208 g          141 t
ORIGIN      
        1 gggcttcacg aaaatcccga ccgcccccgc ctcgccgccg ccgccgccga aactgtcctt
       61 gaccgtccgc agcatatcgc ccaccgtgat gaccagcgcg cttttgccgg cggcgatgac
      121 ttcgcgggcg atgccgcagg caaggtggtt tttgcccgtg cccctcctgc ccgaaaaaat
      181 catgctccgc cccgtctgca acacatcggc gaagtttgcc gcatactcgg cggcggccgc
      241 cttcgccctc gccatccccg ggatcgaatc gctgacggcg tagttttcaa tccggcagtt
      301 tctgaaccgc ggggcgatgc ccgaacgccc gatgcgtttt tccagcgcgt cgcgcatcgc
      361 cccgcggcgc agcgtttccg cgtatgccgc catttcgtcc gccgcctcca gcttccggca
      421 ggccgggcag cccgtccaca cgccgcgcaa aacgcttttc gccgcgtatc cgccgtgttc
      481 cgcgcattgc ctccgctcga cccgcacgcc gccgccgtaa gcccccaaga aatcagacgc
      541 gttcctcaaa gccataccaa gccccctcaa aaatccgttg tcggcatatc gccgtagctt
      601 cccgcatccg gcacggcggc ggtttggttg tgcgtcagac cgccgcggtt tccggacctg
      661 ccgaaagttt tgttttgcag ccagtcggcg cggaagctgc cccagccgct ggcgatggcg
      721 tactccgccg cctgcagcgc cgtcatcccg cattt
//
LOCUS       DAXDSD010000211          753 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXDSD010000211 DAXDSD010000000
VERSION     DAXDSD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.215"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            79..>753
                     /locus_tag="ACFB2Q_002177"
     rRNA            79..>753
                     /locus_tag="ACFB2Q_002177"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          234 a          149 c          224 g          146 t
ORIGIN      
        1 atcaaagtag ggatttcaag tttgcttact tagtcaacgg gtaggtaaac gaagtcaaag
       61 aagttcttga aatgatagag tcaagtgaat aagtgcatca ggcggatgcc ttggcgatga
      121 taggcgacga aggacgtgta agcctgcgaa aagcgcgggg gagctggcaa taaagctatg
      181 attccgcgat gtccgaatgg ggaaacccac tgcattctgt gcagtatcct aagttgaata
      241 cataggctta gagaagcgaa cccggagaac tgaaccatct aagtacccgg aggaaaagaa
      301 atcaaccgag attccgcaag tagtggcgag cgaacgcgga ggagcctgta cgtaataact
      361 gtcgagatag aagaacaagc tgggaagctt gaccatagcg ggtgacagtc ccgtattcga
      421 aatctcaaca gcggtactaa gcgtacgaaa agtagggcgg gacacgtgaa atcctgtctg
      481 aatatggggg gaccatcctc caaggctaaa tactcatcat cgaccgatag tgaaccagta
      541 ccgtgaggga aaggcgaaaa gaaccccggg aggggagtga aacagaacct gaaacctgat
      601 gcatacaaac agtgggagcg ccctagtggt gtgactgcgt accttttgta taatgggtca
      661 acgacttaca ttcagtagcg agcttaaccg gataggggag gcgtagggaa accgagtctt
      721 aatagggcga tgagttgctg ggtgtagacc cga
//
LOCUS       DAXDSD010000212          745 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXDSD010000212 DAXDSD010000000
VERSION     DAXDSD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.216"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..641
                     /locus_tag="ACFB2Q_002178"
     CDS             <1..641
                     /locus_tag="ACFB2Q_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC0919110.1"
                     /translation="EVLSEENNKSVLAAIARNGAIWDKPTRHEDIVAVFPFGNPIHNN
                     TMIMRRSVIDGGLRFDPAYIHAEDYKFWYEAGKLGRLAYYPEALVKYRFHQDQTSSKY
                     NLQQRRTAWKIKEEIRAGYWKAAGISVGADCLNYGLLKSTAYALYEKALSGQDIGCLR
                     LFLYEYFLSLEKYSLTDLLDFLTDRVMRKLFAAPQYRKILKKMLRPWKYRSY"
     gene            660..>745
                     /locus_tag="ACFB2Q_002179"
     CDS             660..>745
                     /locus_tag="ACFB2Q_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC0919111.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIP"
BASE COUNT          200 a          188 c          190 g          167 t
ORIGIN      
        1 tggaagtttt gtcggaagaa aacaataaaa gcgtgcttgc cgccattgcc cgaaacggcg
       61 caatttggga caaaccgacc cggcatgaag acattgtcgc cgttttccct ttcggcaacc
      121 ccatacacaa caacacgatg attatgaggc gcagcgtcat tgacggcggt ttgcggttcg
      181 atccagccta tatccacgcc gaagactata agttttggta cgaagccggc aaactgggca
      241 ggctggctta ttatcccgaa gccttggtca aataccgctt ccatcaagac cagacttctt
      301 ccaaatacaa cctgcaacag cgcaggacgg cgtggaaaat caaagaagaa atcagggcgg
      361 ggtattggaa ggcggcaggc atatccgtcg gggcggactg cctgaattac gggcttttga
      421 aatcaacggc atatgcgttg tacgaaaaag ccttgtccgg acaggatatc ggatgcctcc
      481 gcctgttcct gtacgaatat ttcttgtcgt tggaaaagta ttctttgacc gatttgctgg
      541 atttcttgac agaccgcgtg atgaggaagc tgtttgccgc accgcaatat aggaaaatcc
      601 tgaaaaaaat gttacgccct tggaaatacc gcagctattg aaaccgaaca ggataaatca
      661 tgcaaaacca cgttatcagc ttggcttccg ccgcagagcg cagggcgcac attgccgata
      721 ccttcggcag tcgcggcatc ccgtt
//
LOCUS       DAXDSD010000213          697 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXDSD010000213 DAXDSD010000000
VERSION     DAXDSD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.217"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..258
                     /locus_tag="ACFB2Q_002180"
     CDS             <1..258
                     /locus_tag="ACFB2Q_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HFC0919112.1"
                     /translation="NQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQISAESKIAEL
                     TNKIIPGMSKNERLKIKQKIKNIAKNANRKTKGEEHGRRGR"
     gene            239..694
                     /locus_tag="ACFB2Q_002181"
     CDS             239..694
                     /locus_tag="ACFB2Q_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919113.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
BASE COUNT          260 a          114 c          129 g          194 t
ORIGIN      
        1 aatcaggcgg aaacggaagg ggtttgggtt cataattctt gtccgcctaa aagaacagga
       61 agctccaaga atgaaaaaca tggagacggc ggtcgaagtc aaatatcagc ggaatcaaaa
      121 attgctgaat taacaaataa aattattccc ggaatgtcca aaaatgaacg cttaaagatt
      181 aagcaaaaaa tcaaaaatat tgccaaaaat gccaatcgaa aaacaaaagg agaagagcat
      241 ggtcgacgcg gtcgttaaaa ctcccgaatt tttacctttt acttctgtag gaatattccg
      301 ttttggtgct gatataacac agtacaaaaa catactggaa acttttatgt atgaacctcc
      361 tgatgagttt ggaacagaat attatgaatc tcccgactca aatttactca tttccgtcaa
      421 aaaaaacaaa attatttcga tattttgtta tcaggagcta tattttatgg gcgttaatat
      481 aataggtctg aattttgaag attttaaaca gcttttccat caccctaaat catacggtgt
      541 tgataaatat tatttatcaa atgaatctta tccgacatat gtctatgagt ttgatgaaat
      601 aggagtacaa gcatgggaag caaaagggaa aattgttact atcattgccg ggggcaaaga
      661 caattattcg acagaacctt actacgatga ataacat
//
LOCUS       DAXDSD010000214          696 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXDSD010000214 DAXDSD010000000
VERSION     DAXDSD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.218"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(84..386)
                     /gene="imm40"
                     /locus_tag="ACFB2Q_002182"
     CDS             complement(84..386)
                     /gene="imm40"
                     /locus_tag="ACFB2Q_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm40 family immunity protein"
                     /protein_id="HFC0919114.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPTAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(402..>696)
                     /locus_tag="ACFB2Q_002183"
     CDS             complement(402..>696)
                     /locus_tag="ACFB2Q_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919115.1"
                     /translation="ESKNTVTTNNFFENTGYTEKVLRQASNGDYHGFPQSVDAFSENG
                     TVIQIVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKINHRLFVPNQQLPEK"
BASE COUNT          215 a          126 c           83 g          272 t
ORIGIN      
        1 tccggtcagc gtaggttagg ttaaaaccca gcattttcaa catcttgatg ttttactata
       61 aacctattct tgtgcttcct ttgttagata aatttcaaaa caaaagacac gtatgcattc
      121 tctaatttta tttgagataa ataatgcatt gctttgttga tactgttaaa taaattactt
      181 ccctcgtaat accaattatc atatgtttga taaaaataat tatctttctt gatataaata
      241 tctcccccca atacaagtat tctttcattt ataaataaat ttaatatatc tattgcagtg
      301 gggacgggta atgcggcttc tctaattcca attcctatca aagattcccc ttgtctcgaa
      361 taaatttcaa aaatttcatt taacatatta atctctatct actatttttc aggaagttgt
      421 tggttaggga caaataatct atgattaatt tttccatctg cttctctgat atattcaaaa
      481 tttccatctt ttcctttata agatccaggg atataaagtt tatgccttac aatattatct
      541 ccgccaacta tttgtattac tgttccattt tcagaaaaag catctactga ttgcggaaaa
      601 ccatgataat caccattcga tgcttgcctt aatactttct ctgtataacc ggtattttca
      661 aaaaaattat tcgttgttac agtatttttt gattcc
//
LOCUS       DAXDSD010000215          691 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXDSD010000215 DAXDSD010000000
VERSION     DAXDSD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.219"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            590..>691
                     /locus_tag="ACFB2Q_002184"
     CDS             590..>691
                     /locus_tag="ACFB2Q_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFC0919116.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDY"
BASE COUNT          230 a          191 c          113 g          157 t
ORIGIN      
        1 agccttgtcg gcacggaaac ttatcgggta aaaaggtttc tccggtcctg agtcctggat
       61 tcccactttc gtgggaatga cgggatttaa tgatgccgcc ggcaacgaca aaatcgacac
      121 caagcacctg ccgtcaacct gccgcgataa atcatctgcc gtttgcacga aacaccacgc
      181 gccgatttca aatgctttcc aagaaaacgg agctttttaa aaaataaaaa attcccccac
      241 ccaacccacc ctattctaac acgtaaattc aaaaatctca aattccgacc caatcaacac
      301 acccgatacc ccatgccaat aaaaaagtaa cgaaaatcgg cactaaaact gacaattttc
      361 gacactgccg ccccctactt ccgcaaacca cacccaccta aaagaaaata caaaataaaa
      421 acaattatat agagataaac gcataaaatt tcacctcaaa acataaaatc ggcacgaatc
      481 ttgctttata atacgcagtt gtcgcaacaa aaaaccgatg gttaaataca ttgcatgatg
      541 ccgatggcgt aagcctgagg catttcccct ttcaattagg agtaatttta tgaataccct
      601 tcaaaaaggc tttaccctta tcgagctgat gattgtgatc gccatcgtcg gcattttggc
      661 ggcagtcgcc cttcccgcct accaagacta c
//
LOCUS       DAXDSD010000216          672 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXDSD010000216 DAXDSD010000000
VERSION     DAXDSD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.220"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="ACFB2Q_002185"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="ACFB2Q_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC0919117.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(485..>672)
                     /locus_tag="ACFB2Q_002186"
     CDS             complement(485..>672)
                     /locus_tag="ACFB2Q_002186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919118.1"
                     /translation="LKTLVGSSSFLYLLENKTSSILRIRWMLLPKPSPILPFFGGQAL
                     GKLWIKNILFCSLKNAV"
BASE COUNT          210 a          137 c          146 g          179 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgctgt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacatgaac aaaacttccc gtgaacaatt ggaaatccgc acccatttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactgtcg aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca gatttaaggc
      421 aaaacaacaa aatcaaaaaa cagtgcggtc ggtgaaatcg gcttattttg tgcctcaaga
      481 attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat aactttccta
      541 aggcttgccc cccgaaaaaa ggtagaatcg ggcttggttt gggcagaagc atccatctga
      601 ttctaaggat ggatgatgtt ttattctcaa gtaaataaag gaaagatgat gagcctacta
      661 atgtctttaa ag
//
LOCUS       DAXDSD010000217          668 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXDSD010000217 DAXDSD010000000
VERSION     DAXDSD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.221"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(116..667)
                     /locus_tag="ACFB2Q_002187"
     CDS             complement(116..667)
                     /locus_tag="ACFB2Q_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919119.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCIIGLV
                     GRFRLQTASGRVAKAGGAVKAGG"
BASE COUNT          184 a          261 c          125 g           98 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaagcaaag aaaccgccat tagcactaca cccccccaag
       61 ctgcggctgc caaacacatc ggctgcttct cgacgcaccc gctgaaaaat gcgccctatc
      121 cgcccgcctt gaccgcaccc cctgcctttg ccaccctgcc cgatgccgtc tgaagcctga
      181 accgccctac cagcccgatg atgcacgaca ccgccggaaa gggcatacac aaagccgtca
      241 gcacataacg cgcattcccc gtattgacca cgtccgcgcc cttcagcagc agcggcagcg
      301 cgttcgccca ctcccacagc gaaggcgcgc tgaacgccaa taccgccacc agccccaaca
      361 tccggaacac cccgatttcc aagacctgcc gccgccgcac cgaacggttg agcaaaaaca
      421 caaccgccaa cccgaaacag accaccgccg ccataccgcc gttggcgaca atctgcaaac
      481 tgttgaacgc aatatcgggc gtaaccgcca acacgccttc cgaatccggc acggcacgct
      541 gcaaatccaa cccctgcaac acattcaaca caaaaccgta gatcaaatca aaaaacacca
      601 cgcctatcgg caaagcactc aaaacccgcc acatcttcct atctgaccaa ccgttcaaaa
      661 ccttcatt
//
LOCUS       DAXDSD010000218          627 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXDSD010000218 DAXDSD010000000
VERSION     DAXDSD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.222"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>627
                     /locus_tag="ACFB2Q_002188"
     CDS             <1..>627
                     /locus_tag="ACFB2Q_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC0919120.1"
                     /translation="CLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKT
                     STWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFAQKATRIHAVKEDKVVSAD
                     RCLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLALEAYDCAFLKSQV
                     IHEQPAFKPYNDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFAR"
BASE COUNT          203 a          141 c          139 g          144 t
ORIGIN      
        1 tgtttggggt cggattggga agaagacgat gaagccaaaa tgaccaaagg caagacctat
       61 ggtatcggct cccgtgaatc gtccaaatat gtccgcgtct atgaaaaagg caagcagttg
      121 ggcgataaaa caagcacatg gacgcgcttt gaaattgaat tcaaagcaaa agacatcgtt
      181 atccctttcg aagttttgca gaatccgggc gaatatttcg gcggcgcata tcccatttgc
      241 gaacgattcg cccaaaaggc aacgcgcata cacgcggtta aggaagataa ggttgtttca
      301 gccgaccgct gccttgaatg ggtaaaaaaa cagttcggac gtgcggcaaa cggtctgaaa
      361 ttcatttttc ccgaattgga caaagccaaa ctgtttgaac tgattgagcc gagtcatcac
      421 aagctgccca agtctttggc tctcgaagcc tacgactgcg cctttttgaa atctcaagtc
      481 attcatgaac agcccgcatt caaaccgtac aatgaccctt attatatgta cgaatattac
      541 gagaatcttg aaaaacagct tgaacagcaa aaacacgtca acaatgaaga aagctataac
      601 aacttcattt acgacaaatt cgcaaga
//
LOCUS       DAXDSD010000219          611 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXDSD010000219 DAXDSD010000000
VERSION     DAXDSD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.223"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..501
                     /locus_tag="ACFB2Q_002189"
     CDS             <1..501
                     /locus_tag="ACFB2Q_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919121.1"
                     /translation="ASKWQVRGCSRCRADRQAGERQPRDGNGRWTAGEVVSVSGGELG
                     EFAETKELRKAAMQYARDNFVGKSYVNESSGHELKVTWQGVKHAASKSNSIELSLMVA
                     LDKVLMNAKYEGSFPNKLGRDHIIAVHKYSTGVRFGNKVLKIRIIAREKFDGIKHYDH
                     FILKDK"
BASE COUNT          188 a          112 c          163 g          148 t
ORIGIN      
        1 gcatccaaat ggcaggtgcg cggttgttcc cgttgtcggg cggacaggca agctggcgaa
       61 aggcagccgc gcgatggaaa cgggcgatgg acggcaggtg aggttgtttc cgtatcgggc
      121 ggcgaattgg gggaatttgc tgaaacaaag gagcttcgta aagcggctat gcagtatgcg
      181 cgtgataact ttgtcggcaa aagctatgtc aatgaaagca gcgggcatga actgaaggta
      241 acttggcaag gtgtgaaaca cgctgcgtca aaatcgaaca gcatcgaact ttcccttatg
      301 gtcgcgcttg ataaagtttt gatgaatgca aaatatgaag gaagttttcc taacaaatta
      361 gggagggatc atattattgc ggtacacaaa tactcaaccg gtgtcagatt tggcaataaa
      421 gtcttaaaaa tcaggataat agcccgtgag aaatttgatg ggataaaaca ttacgatcat
      481 tttattttga aagacaaatg aaaaaaccct gtccagtata tctgggatag cttatggaag
      541 catttaaccc agcctcggat aaggctttaa atccatcatg ccatttatac ggtaaaaaca
      601 agggtctgta c
//
LOCUS       DAXDSD010000220          611 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXDSD010000220 DAXDSD010000000
VERSION     DAXDSD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.224"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            89..318
                     /locus_tag="ACFB2Q_002190"
                     /pseudo
     CDS             89..318
                     /locus_tag="ACFB2Q_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(355..>611)
                     /locus_tag="ACFB2Q_002191"
     CDS             complement(355..>611)
                     /locus_tag="ACFB2Q_002191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919122.1"
                     /translation="GLYYKIWEGGWRMPALPFFRTSIPMLVQNRMESIFNHNKDAIFL
                     EGWSLGDAAMIMELLWQNISTSRRELTKLFLYKNFWGCTR"
BASE COUNT          187 a          118 c          120 g          186 t
ORIGIN      
        1 gttacgcatg ggattaccct tatatattgc gtccctaaga aggggcgatt aacaaaaatt
       61 aatgcccttt cttagggacg caatatataa ggtttatacc gtcgttgtcc ctaatgcgca
      121 atcagcgaca ttgttgccaa ttatccgaaa gaaagttaag cctgatggca ttgtatacac
      181 ggataccttt cgtagttatg atgtgcttga tgttagtgaa tttagccatt tacgtaagtt
      241 taacggcatt cccaaagagc atttggggct atatttaaag aaatgccaat ggcatttgaa
      301 atagaatctc aaatttagat tttaagacaa ttagttaatg ggaatttggt ctggttatct
      361 agtacaaccc caaaagtttt tatataaaaa cagtttggta agttctcttc gtgaagtgct
      421 tatgttttgc cacaaaagtt ccataatcat ggcggcatca cccaagctcc atccttccaa
      481 gaaaatggca tctttgttgt ggttgaaaat cgactccata cgattttgga caagcatagg
      541 gatagatgtt ctgaaaaacg gcagcgcggg cattctccaa ccaccttccc aaatcttata
      601 atacaaaccc g
//
LOCUS       DAXDSD010000221          606 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXDSD010000221 DAXDSD010000000
VERSION     DAXDSD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.225"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>606
                     /locus_tag="ACFB2Q_002192"
     rRNA            <1..>606
                     /locus_tag="ACFB2Q_002192"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          126 c          186 g          122 t
ORIGIN      
        1 ggtactggag gaccgaaccc acgcatgttg caaaatgcgg ggatgagctg tgggtagggg
       61 tgaaaggcta aacaaactcg gagatagctg gttctccccg aaaactattt aggtagtgcc
      121 tcgagcaaga cactgatggg ggtaaagcac tgttatggct agggggttat tgcaacttac
      181 caacccatgg caaactcaga ataccatcaa gtggttcctc gggagacaga cagcgggtgc
      241 taacgtccgt tgtcaagagg gaaacaaccc agaccgccgg ctaaggtccc aaatgataga
      301 ttaagtggta aacgaagtgg gaaggcacag acagccagga tgttggctta gaagcagcca
      361 tcatttaaag aaagcgtaat agctcactgg tcgagtcgtc ctgcgcggaa gatgtaacgg
      421 ggctcaaatc tataaccgaa gctgcggatg ccggtttacc ggcatggtag gggagcgttc
      481 tgtaggctga tgaaggtgca ttgtaaagtg tgctggaggt atcagaagtg cgaatgttga
      541 catgagtagc gataaagcgg gtgaaaagcc cgctcgccga aagcccaagg tttcctacgc
      601 aacgtt
//
LOCUS       DAXDSD010000222          603 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXDSD010000222 DAXDSD010000000
VERSION     DAXDSD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.226"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..429
                     /locus_tag="ACFB2Q_002193"
     CDS             <1..429
                     /locus_tag="ACFB2Q_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC0919123.1"
                     /translation="NPDTDGQPGTSPDSPAVPDRPNGRHRKERKEGEDGGLSCDYFPE
                     ILACQEMGKPSDRMFHDISIPQVTDDKTWSSHNFLPSNGVCPQPKTFHVFGRQYRASY
                     EPLCVFAEKIRFAVLLAFIIMSAFVVFGSLGGNKCHYLPA"
     gene            408..>603
                     /locus_tag="ACFB2Q_002194"
     CDS             408..>603
                     /locus_tag="ACFB2Q_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 family protein"
                     /protein_id="HFC0919124.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLAALEKFK
                     GYTANAINSMPSDILNLLLIS"
BASE COUNT          150 a          155 c          142 g          156 t
ORIGIN      
        1 aatcccgata cggacggaca gcccggaaca agccccgatt ccccggccgt tccggaccgc
       61 ccaaacggca ggcatcgcaa agaaaggaaa gaaggcgaag acggcgggct ttcgtgcgat
      121 tattttccgg aaatcctagc ctgtcaggag atgggcaaac cttcggaccg catgtttcac
      181 gatataagca taccgcaggt tacagacgat aaaacatggt cttcacataa ctttttaccg
      241 tctaacggcg tatgtccgca gccgaaaacc tttcatgttt tcggcaggca atatcgggca
      301 agctatgaac cgttgtgcgt gtttgccgaa aaaatccgtt ttgccgtact gctcgccttt
      361 atcattatgt cggcttttgt cgttttcggt tcgttggggg ggaataaatg ccattacttg
      421 ccggcctgat tccactttta ggcatacttc tgaaaatgct gattgtcaga atcatccttg
      481 caacaggtct gacattcgta acctatgccg ggtatctcgc cgcactggaa aagttcaaag
      541 gctacacggc aaatgcgatc aattccatgc cttccgacat attgaacctt cttttaattt
      601 cgg
//
LOCUS       DAXDSD010000223          596 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXDSD010000223 DAXDSD010000000
VERSION     DAXDSD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.227"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            44..334
                     /locus_tag="ACFB2Q_002195"
     CDS             44..334
                     /locus_tag="ACFB2Q_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919125.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVTGFQFVKKG"
     gene            339..536
                     /locus_tag="ACFB2Q_002196"
     CDS             339..536
                     /locus_tag="ACFB2Q_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919126.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
BASE COUNT          180 a          147 c          124 g          145 t
ORIGIN      
        1 gtgtctgccc gaaagacgtt taatcacaca aggaaaccaa aaaatgaaca tccaacttca
       61 aggccacatc gtcggcgtta aaaaattcaa cggacaaatc gaaggcaaga gcttcgacta
      121 ttgccgcctg attgtcgcca cacccttaga cagctcccaa ggcaacgcat tgggcagctc
      181 tactactgaa tacgatttcg gcggctctgc caatttcgag cagttccgaa acgcccaatt
      241 tccgatcgaa gcaaacctga acgtggaaat cgtcactacg ggcaaaactc aaaaactgaa
      301 agtcaccggt tttcaattcg ttaagaaagg ctgattgaat gcagaaagtc tatgttgtcc
      361 agtccgtatc aacaggggac tttctgtatc tctctcctga aacgggcgac atcggacata
      421 ccaaattaat taccaatgcc gattatttct acgacttcga agaagcggtt aacgcaggtt
      481 tggaagaaat cggcaaccaa tgcgaatttg tcgtattcgg atttttgaaa gactgatttt
      541 cggatgttcg gcggtcgtct gaaaaactct ccatccatta ccgccaaaca cttttt
//
LOCUS       DAXDSD010000224          584 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXDSD010000224 DAXDSD010000000
VERSION     DAXDSD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.228"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(117..>584)
                     /locus_tag="ACFB2Q_002197"
     CDS             complement(117..>584)
                     /locus_tag="ACFB2Q_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFC0919127.1"
                     /translation="TIRKYNDDGCGAETAILGINTADGGALTPRSARPIVPDHNSVAQ
                     YSGHKKTADGKSVPIGCMWKNGKTVCPNGYVYDKPVNVRYLDEKKTDDFPVTADGDAG
                     GSGTFKEGKKPARNNRCFSGKGVRTLLMNDLDSLDITGPMCGIKRLSWREVFF"
BASE COUNT          113 a          166 c          162 g          143 t
ORIGIN      
        1 cccgcctgcg cgggaatgac gcgtttcatt tgccgccccc tcccgaaaaa cgcaaaaaaa
       61 tgccgtccga aggcctttcg gacggcattt gcggaaaaac cggccgcgca ggcgggtcag
      121 aagaagactt cgcgccagct taagcgtttg ataccgcaca tcgggccggt aatatccaag
      181 ctgtccaaat cgttcatcag cagggtgcgc acaccttttc cggagaagca ccggttattg
      241 cgggcgggtt ttttaccctc tttgaatgtt ccgctgccgc ctgcatcacc gtctgccgtg
      301 acggggaaat cgtctgtttt cttttcgtcc aggtagcgca cattaaccgg tttgtcgtaa
      361 acatatccgt tcgggcagac ggttttgccg tttttccaca tacaacctat agggacggat
      421 ttgccgtcgg cggttttctt atggccggaa tattgcgcaa ccgaattgtg atccggcaca
      481 atcgggcgcg cgcttctcgg agtcaatgcg ccgccgtcgg cggtattgat gcccaaaatg
      541 gcggtttccg cgccgcagcc gtcgtcgtta tatttgcgga tggt
//
LOCUS       DAXDSD010000225          568 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXDSD010000225 DAXDSD010000000
VERSION     DAXDSD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.229"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            244..>568
                     /locus_tag="ACFB2Q_002198"
     CDS             244..>568
                     /locus_tag="ACFB2Q_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919128.1"
                     /translation="MTKLYAEIAEMEAQDDGTVKVWGYASSEAVDSDGEVIAAAAMKA
                     AIPDYMKFGEVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVEKVKTGVYKGFS
                     IGGSVT"
BASE COUNT          161 a          128 c          162 g          117 t
ORIGIN      
        1 tagggaggga tgtcgtgata tttgaaaaat caataaacgg ctggaaatac cgcgccgtaa
       61 tggaaatcag aaccacgcaa aaggagcttg ccttgaacag cctttataca acagacagga
      121 ttgaatagtc atgacaaaac ctgcgacgag taactgtcaa tctttcgatt ccgtcctcat
      181 acatcctaag cgtctttgca aattctgttc agttatacca tttatacaga caggagcaaa
      241 ccaatgacga agttatacgc agaaatcgcc gaaatggagg cgcaggacga cggcacggtc
      301 aaagtttggg gttatgcctc aagcgaggcg gtcgattcgg acggcgaagt catcgcggcg
      361 gcggctatga aggcggcgat tcccgattat atgaagttcg gcgaggtgcg cgagatgcac
      421 ggcagcaacg cggcgggaac ggcgattgaa atcaacgtgg aagacgacgg cagaaccttt
      481 ttcggggcgc atatcgtcga tcccgttgca gtggagaaag tcaaaacggg cgtttacaag
      541 ggcttttcca tcggcggcag cgttaccg
//
LOCUS       DAXDSD010000226          558 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXDSD010000226 DAXDSD010000000
VERSION     DAXDSD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.230"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            38..532
                     /locus_tag="ACFB2Q_002199"
     CDS             38..532
                     /locus_tag="ACFB2Q_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919129.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKDLFAEEVPENNIVCDISHFLHFADRA
                     EADIPNQSRLYAEYWWEMETFKMSVLKDWERSGRPLDWEKWQRQYQSEAFELVKNLNR
                     SKQE"
BASE COUNT          193 a           94 c          123 g          148 t
ORIGIN      
        1 aatttaatcc actataattt ggaagaggca gcaacctatg gaaaatagat ttgaaaaaat
       61 cgttaaggat atttttagtt ttacccaatt tgtttccgaa gcggaaaata aaattcctaa
      121 ccaatcctcc cgttattacg actgctggtg ggaattggaa atcctgaatg cctccgcatt
      181 ggaagattgg gaaatatcgg gaaagcctga aaaatgggag aaatggacag aaaaatatca
      241 gaccgaagcg ttacagtcag ttaaggatct atttgccgaa gaagttcctg aaaataatat
      301 agtttgcgat atttctcatt ttttgcattt tgccgatcgg gcagaagccg acattcccaa
      361 tcaatcacgt ttatatgccg aatattggtg ggaaatggaa acgttcaaaa tgtcggtttt
      421 aaaagattgg gagcgttcag gcagaccact agattgggaa aaatggcaaa ggcaatatca
      481 atccgaagca tttgaattag tcaaaaattt gaaccgaagc aaacaagagt aacaagccgc
      541 agcctgcata ggggtttt
//
LOCUS       DAXDSD010000227          550 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXDSD010000227 DAXDSD010000000
VERSION     DAXDSD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.231"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>550
                     /gene="mafB"
                     /locus_tag="ACFB2Q_002200"
     CDS             <1..>550
                     /gene="mafB"
                     /locus_tag="ACFB2Q_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC0919130.1"
                     /translation="QWADAHPNITATAQTALAVAEAAATVWRGKKVKLNPTKWDWVKN
                     TGYKKPAARPMQTVDGEMAGKNKPPKPSTQQHSTHSDNNIGLPAPYVKPDTSISPTGT
                     IQDRIRWTKSKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQT
                     EHRRVRYDINNNIYVLANPKTFK"
BASE COUNT          196 a          126 c          107 g          121 t
ORIGIN      
        1 gcaatgggct gatgcccatc cgaacattac agctactgcc caaactgccc ttgccgtagc
       61 agaggccgca gctacggttt ggagaggtaa aaaagtaaaa cttaacccga ccaaatggga
      121 ttgggttaaa aataccggct ataaaaaacc tgctgcccga cctatgcaga ctgtagacgg
      181 ggaaatggcc gggaaaaata agccaccgaa accaagtacg cagcaacact ctacacactc
      241 tgataacaat atcggcttac ctgccccata tgttaaacct gatacatcta tttctccgac
      301 aggaacaatt caagaccgca tcagatggac aaaatccaag tttcctactg agaaatcttt
      361 aaatggacat ttcaaagctc atggaaaaga atttggcgat ataaccattg aagactacca
      421 aaaaatggcg tctgatttgt tatcaaaaca gacatcggac aagatattag gttatcagac
      481 ggaacataga cgagtgcgct atgatatcaa taacaatatc tatgttttgg ccaatccaaa
      541 aacattcaaa
//
LOCUS       DAXDSD010000228          540 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXDSD010000228 DAXDSD010000000
VERSION     DAXDSD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.232"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..186)
                     /locus_tag="ACFB2Q_002201"
     CDS             complement(<1..186)
                     /locus_tag="ACFB2Q_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HFC0919131.1"
                     /translation="MLGLCLLTPASYQNIHVRSWKARRDSGIVKQDLDFSCGAASIAT
                     LLNNFYGRHYSEAEILDK"
BASE COUNT          216 a           84 c           83 g          157 t
ORIGIN      
        1 tttgtctaag atttccgctt cagaataatg tctgccataa aaattattca gtaacgtggc
       61 aatcgaagcc gcgccgcaag aaaaatccaa atcttgtttt actatgccgg aatctcgccg
      121 tgctttccaa ctccgtacat ggatgttttg gtaagaagcg ggggtcaaca aacataggcc
      181 aagcaaaaac tatatttggg gcgaaaccaa tcaaagccgc ataatttatc aatttataaa
      241 gattttttat cataatatgt atacgcggaa taaacgcatg aaacataaaa aaataaaatc
      301 atatgcaatt ttattgcatg aataaatatg aataaaatag ataatgatgg gagtaaatac
      361 gccatgtatt ttggaagttt aaatttatta ataataaaat aatttatcgt agcgcaaata
      421 aatcccaaaa ttggaacgat tagaaaaaaa attaatacac ccataatcat gcaacctata
      481 ttaataaata gctaactaat tctattacag aaattagtta gctgattaaa agtgattaac
//
LOCUS       DAXDSD010000229          521 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXDSD010000229 DAXDSD010000000
VERSION     DAXDSD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.233"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>521
                     /locus_tag="ACFB2Q_002202"
     CDS             <1..>521
                     /locus_tag="ACFB2Q_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272554.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC0919132.1"
                     /translation="NTREAVDRWIQENPNAAETVEALVNILPFSKVKGLVKAAKLGRA
                     AKPGRAAKLGKAKVSEDFAVASQARYTSQTAENIIAADRIGSGLKADTSHRAASFLSQ
                     EQLGAGRTFSITGGDGIQRTLLQTKGSLNGQSGIYEYILDSNNKVTHQRFIKGGEITG
                     QPNQRVKTKVRNRG"
BASE COUNT          176 a          106 c          136 g          103 t
ORIGIN      
        1 aatacgcgcg aagccgttga ccggtggata caggaaaacc ccaatgccgc cgaaaccgtc
       61 gaagccctgg tcaacatcct gccgttttcc aaagtcaaag gattggtgaa ggcggcgaaa
      121 ctggggaggg cggcaaaacc ggggagggct gcgaaactag ggaaggcaaa ggttagcgag
      181 gattttgctg ttgccagtca ggcaagatat acttcgcaaa cggcagaaaa tataattgca
      241 gccgatagaa taggaagtgg attaaaagct gatacgtcac atcgtgcagc cagctttcta
      301 tctcaagaac aattgggagc aggaagaaca ttctcaatta ctggcggcga tggtattcaa
      361 agaactttat tgcagacaaa aggttctttg aacggtcaaa gcggtatata tgaatatatc
      421 ctcgattcaa ataacaaagt gactcatcaa agattcatta aaggaggtga aattacagga
      481 caaccaaacc aacgcgtgaa aactaaagta cgcaaccggg g
//
LOCUS       DAXDSD010000230          520 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXDSD010000230 DAXDSD010000000
VERSION     DAXDSD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.234"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT          114 a          142 c          145 g          119 t
ORIGIN      
        1 ggcgggaatc cagtccgttc ggtttcagtc atttccaaaa attgccgtag cgttaagttt
       61 ctagattccc actttcgggg gaatgacgcg gtgcaggttt ccgtgcggac ggattcgtca
      121 ttcccacaca gacaggaatc cggatttgtc cgcgcggaaa cttatgcgcc gtcattcccg
      181 cacaggcggg aattcaggtc tgtcggcata ggaacttatc ggataaaacg gttgccccaa
      241 ccctgcgttc tagattccca ctttcgtggg aatgacgatt caggtattcc taatagcaac
      301 ccgccacaac ccacctacgc ccatcctacg catctgccat acagccctgt tccggagcgc
      361 gcggataagg tgcgatgccg ccggcgggca tcatagtcct ctctccctgt gggagagagc
      421 tagaaagctg caaggcttgt attttagaag actaagggat ttgggaaaga ttgtcgaaat
      481 tcggggagcc ctctccccca cgggggaggg ggcaggttgc
//
LOCUS       DAXDSD010000231          517 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXDSD010000231 DAXDSD010000000
VERSION     DAXDSD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.235"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..509
                     /locus_tag="ACFB2Q_002203"
     CDS             <1..509
                     /locus_tag="ACFB2Q_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919133.1"
                     /translation="KKVPWAVWALIAVVGFVGWKSYGMFQVYSKATDSRIEQEAQKES
                     VVQTMTEQTASSETAPFEHSDNLKPEDFVPTLPEKPESKPIYNTVRQVKTFEQIAGCI
                     DGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYKDERQRTEQAAQSAKADKPQ
                     VLVMGGKS"
BASE COUNT          175 a          107 c          133 g          102 t
ORIGIN      
        1 agaaaaaagt gccttgggcg gtttgggcgt tgattgcggt tgtagggttt gtaggctgga
       61 aaagttacgg catgtttcaa gtttacagca aagccacaga cagccggatt gagcaggaag
      121 cgcaaaaaga aagcgttgtg cagacgatga cggagcagac ggcatcatca gaaacagcgc
      181 cttttgagca ttccgacaat ctgaaacctg aagactttgt gccgactttg cccgaaaagc
      241 ccgaaagcaa gcctatttat aacacagtcc gacaagtaaa aacctttgag caaatcgccg
      301 gatgcataga cggcggaaaa tcagattgca catgctattc aaatcaagga acacccttga
      361 aagaaataac aaagataatg tgtaaagaat atgtgaaaaa cgggttgcct ttcaatcctt
      421 acaaggacga acggcaaagg acggaacagg cggcacagtc cgcgaaagcg gacaagcccc
      481 aagttctcgt aatgggcgga aagtcctaat aaaattt
//
LOCUS       DAXDSD010000232          511 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXDSD010000232 DAXDSD010000000
VERSION     DAXDSD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.236"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            173..355
                     /locus_tag="ACFB2Q_002204"
     CDS             173..355
                     /locus_tag="ACFB2Q_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919134.1"
                     /translation="MANIDLTQWDGKTIGAAANPEQGYINITIGSDDLFINIEQAYAI
                     HAALGEAVAEYEGGAQ"
     gene            352..>511
                     /locus_tag="ACFB2Q_002205"
     CDS             352..>511
                     /locus_tag="ACFB2Q_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFC0919135.1"
                     /translation="MTALTLYRCAADVQAALDYYFDSETEREDTLEAVIGQFEVKAQS
                     VIAYIKNQE"
BASE COUNT          151 a          148 c          133 g           79 t
ORIGIN      
        1 gaagacatcg aggacgacat cccttttaat taagcccgcc gtcagggcgt aaaaccggac
       61 ggaaaaccgc aaacaaacgc cgcgccctgc gggcgggccc cgcactctga agcagtagtc
      121 gatttttagt ttgcaactgc ttcggacggc aacagagaaa ggaaacaaaa aaatggcaaa
      181 catcgacctg acccaatggg acgggaaaac cattggcgcc gccgccaatc ccgaacaggg
      241 ctacatcaac atcaccatcg gcagcgacga cctattcatc aacatcgaac aggcatacgc
      301 catacacgcc gcgcttggcg aagcggttgc cgaatatgag ggaggggcac aatgaccgcc
      361 ctcacactct accggtgcgc ggcagacgta caggcggcgc ttgattacta cttcgacagc
      421 gaaaccgagc gcgaagacac gctggaagcc gttatcgggc agttcgaggt caaagcgcaa
      481 tccgttatcg cttatattaa aaaccaagaa a
//
LOCUS       DAXDSD010000233          493 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXDSD010000233 DAXDSD010000000
VERSION     DAXDSD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.237"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>493
                     /locus_tag="ACFB2Q_002206"
     CDS             <1..>493
                     /locus_tag="ACFB2Q_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HFC0919136.1"
                     /translation="VLRSITDDQDRQKHFFMFGAMGLGGRGAYALDLSKIDSNPVGVS
                     MFDVQNESKNNGVKLGYTVGTPQIGKTQNGKYAAFLASGYAAKQIASQENKTALYVYD
                     LGNGSGSLIKKIEVQGGKGGLSSPTLVDKDLDGIVDIAYAGDRGGNMYRFDLSDSDSS
                     KWSV"
BASE COUNT          135 a          114 c          133 g          111 t
ORIGIN      
        1 tgtcttgcgc agcattacag atgaccaaga caggcaaaaa catttcttta tgttcggcgc
       61 aatgggcctt ggcggcagag gcgcatacgc cttggattta agcaaaatcg acagcaaccc
      121 ggtcggcgtt tccatgtttg atgtccaaaa cgagagtaaa aataatggcg tgaaattagg
      181 ctacaccgtc ggtacgccgc aaatcggcaa aacccaaaac ggcaaatacg ccgccttcct
      241 cgcctccggt tatgcggcta aacaaattgc cagccaagaa aataaaaccg cgctgtatgt
      301 gtatgatttg ggaaacggca gtggtagtct gattaaaaaa atcgaagtgc agggcggcaa
      361 gggcgggctt tcgtccccca cgctggtgga taaagatttg gacggcattg tcgatatcgc
      421 ctatgccggc gaccggggcg ggaatatgta ccgctttgat ttgagcgatt ccgattctag
      481 taaatggtct gta
//
LOCUS       DAXDSD010000234          483 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXDSD010000234 DAXDSD010000000
VERSION     DAXDSD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.238"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            81..296
                     /locus_tag="ACFB2Q_002207"
     CDS             81..296
                     /locus_tag="ACFB2Q_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919137.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEGYIDEIRDKADEADT
                     SLEFLKDVINYGISDLKNVIEVFEDCV"
BASE COUNT          188 a           70 c          116 g          109 t
ORIGIN      
        1 aagggcggaa gaatttgcag attagataaa ccgtagaaga tgttgacgac agcatcccat
       61 tttgagttaa ggagcaaaaa atgagctatt tggaagatgt aaaaaacgca ttaagggtaa
      121 tagataactt atgcaaagaa gcattaaaag agcctgaatc gttagagggt tatatagacg
      181 aaattaggga taaagcagac gaagcggata cctctttgga atttctaaag gatgtaataa
      241 attatggtat tagcgattta aaaaatgtaa ttgaggtgtt tgaagattgc gtttgatatt
      301 aatggaacaa gacatgaact atcaatttaa attcggcgac cccgtgagag atacagcttt
      361 atgattgggc attgagagaa cggcatcaaa aaatcgagaa aacaaaatgc aaacagtagc
      421 aacaaaaccg acggcaaaac agatgcttgc cgccaaacgc gcggcgaagg aatcaacgcg
      481 gca
//
LOCUS       DAXDSD010000235          483 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXDSD010000235 DAXDSD010000000
VERSION     DAXDSD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.239"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..398
                     /locus_tag="ACFB2Q_002208"
     CDS             <1..398
                     /locus_tag="ACFB2Q_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC0919138.1"
                     /translation="YRLHANQVSSKHSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSL
                     EYRQTKAAAYELPEKDLPEEDFERARRFLYRCFKRTDTPPSGAWLDFAADGRMRRLFT
                     LRQYFGILYRLIKNRRQARSDSAGKEQGI"
     gene            398..>483
                     /locus_tag="ACFB2Q_002209"
     CDS             398..>483
                     /locus_tag="ACFB2Q_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC0919139.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIP"
BASE COUNT          123 a          135 c          131 g           94 t
ORIGIN      
        1 aataccgcct tcacgccaat caggtttcat ccaaacacag cgtccgccaa cacgaaatcg
       61 cgcaaggcat ccaaaaaacc gccagaaacg attttttgca gtctatgggt tttaaaaccc
      121 ggttcgacag cctagaatac cgccaaacaa aagcagcggc gtacgaattg ccggagaagg
      181 atttgccgga agaagatttt gaacgcgccc gccggttttt gtaccgatgc ttcaaacgga
      241 cggacacgcc gccctccggc gcgtggctgg atttcgcggc agacggcagg atgaggcggc
      301 tgtttacctt gaggcaatac ttcggcattt tgtaccggct gattaaaaac cgccggcagg
      361 cgcggtcgga ttcggcaggg aaagaacagg ggatttaatg caaaaccacg ttatcagctt
      421 ggcttccgcc gcagaacgca gggcgcacat tgccgatacc ttcggcagtc gcggcatccc
      481 gtt
//
LOCUS       DAXDSD010000236          478 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXDSD010000236 DAXDSD010000000
VERSION     DAXDSD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.240"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..>478)
                     /gene="mafA"
                     /locus_tag="ACFB2Q_002210"
     CDS             complement(<1..>478)
                     /gene="mafA"
                     /locus_tag="ACFB2Q_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC0919140.1"
                     /translation="RLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARA
                     AVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYP
                     RYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNET"
BASE COUNT          119 a          117 c          122 g          120 t
ORIGIN      
        1 aaggtttcat ttcgataatc ccccatcccg ccaatattta agcccagact gctcctactt
       61 ccgctaccgt ctgattgggt gcgcgagagt gcaggggcat taagtgtaga taaagaagtg
      121 gttaaacctg tcaaaccgcc tgatgttgtt tcagcggtgg tttcgtaacg cggataggtg
      181 taatcggtac ggacggcagg gctgtttatg tattcgccgc gaatcagtgc atcaatggag
      241 tagcgacccc ctgtcaaact gcctgaacct tggtcgccca tagttgcaat gtacaatgca
      301 acttttcgtc cgtgtaatgc ctgtaaatcc atgtctttaa cggcagctct ggcagaagcg
      361 gccacaagtt cttgttcgac cgcgaagcgt ttgcctccgc catgcgatgg aatacctgtc
      421 agtgtcccgc aggcggataa aataaaaact gaaaaaagaa taggtatcag cagccgtg
//
LOCUS       DAXDSD010000237          478 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXDSD010000237 DAXDSD010000000
VERSION     DAXDSD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.241"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..149)
                     /locus_tag="ACFB2Q_002211"
     CDS             complement(<1..149)
                     /locus_tag="ACFB2Q_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC0919141.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFED"
BASE COUNT          117 a          109 c           94 g          158 t
ORIGIN      
        1 tcgtcttcaa aataccaaga ctttgccgcg ccggggaagc cgcccgtctc tttgagccat
       61 tgcacaatcg tcggattgtg tttcgcgccg ggaatttctt tcaaaccgat gtgccttctc
      121 gcttcggcta tccacggtaa ttctgtcatt tttaatcctt tcggtaaaag acgagatgta
      181 aactttgggt tggcaactcg ttctatgttt taagtcgcaa tcacaatgtg caccgcatcg
      241 ccgaacacgc tgtctttttc agtcgcgttt gccgatacgg taactttccg cgcagatgat
      301 atgttgcccg tcaacacaaa ataaaaaaca aagttgcaac atgctgattt atattgttat
      361 ttttatttac gtttatttac gatatgcaaa tgcacagtta cacaaatata ttcgcgcaac
      421 cgtttaattt tgttgaattt tattgattca atcggtgtct ttccgcatcg taaggctg
//
LOCUS       DAXDSD010000238          474 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXDSD010000238 DAXDSD010000000
VERSION     DAXDSD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.242"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..327
                     /locus_tag="ACFB2Q_002212"
     CDS             <1..327
                     /locus_tag="ACFB2Q_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFC0919142.1"
                     /translation="KWYDDGVWDDEEYWKLENALIEVRKKYPYPMDIPRDIVIGIGTI
                     IDFLMVQNWKLFEIKASPWLPKSVKINERYERFRVMLRYIFTDLDAEDWKFFYFPIQH
                     SKGRLR"
     gene            358..>474
                     /locus_tag="ACFB2Q_002213"
     CDS             358..>474
                     /locus_tag="ACFB2Q_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC0919143.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWK"
BASE COUNT          159 a           46 c          112 g          157 t
ORIGIN      
        1 aaatggtatg atgacggagt gtgggatgat gaagaatatt ggaaattgga gaatgcttta
       61 atcgaggtta gaaaaaaata tccttatccg atggatatac caagggacat cgtgattgga
      121 atcggtacca ttattgattt tttaatggtt caaaattgga aactttttga aattaaagct
      181 tctccttggt tgcctaaaag tgtcaaaatt aatgagcgtt atgaaaggtt cagagtaatg
      241 ctccgttata tttttactga tctagatgca gaggactgga aattttttta ctttccaata
      301 caacatagta aaggtagatt gagatgaaaa gtttaattat attggagata cattattatg
      361 tgtgagttca aggatattat aagaaacgtt ccttattttg aggggtatga cgaaaattca
      421 tttattggca aatggtatga tgacggggtg tgggatgatg aagaatattg gaag
//
LOCUS       DAXDSD010000239          466 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXDSD010000239 DAXDSD010000000
VERSION     DAXDSD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.243"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>466
                     /locus_tag="ACFB2Q_002214"
     CDS             <1..>466
                     /locus_tag="ACFB2Q_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC0919144.1"
                     /translation="TTISTVSDYFRNIRTHSIHPRVSVGYDFGGWRIAADYARYRKWS
                     DNKYSVSIKNMRVHKHNSNRKNLKTENQENGSFHAVSSLGLSAIYDFQINDKFKPYIG
                     ARVAYGHVRHSIDSTKKITGLLTTSTPGIMSGVYKVLRTPGAHRESDSIRRVG"
BASE COUNT          144 a          121 c          105 g           96 t
ORIGIN      
        1 cacaacaata agcacggtaa gcgattattt cagaaacatc cgtacgcatt ccatccaccc
       61 ccgggtgtcg gtcggctacg acttcggtgg ctggaggata gcggcagatt atgcccgtta
      121 caggaaatgg agcgacaata aatattccgt cagcataaaa aatatgcggg tacataaaca
      181 caatagcaac aggaaaaacc tgaagacgga aaatcaggaa aacggcagct tccacgccgt
      241 ttcttctctc ggcttatccg ctatttacga tttccaaata aacgataaat tcaaacccta
      301 tatcggcgcg cgcgtcgcct acggacacgt cagacacagc atcgattcga ctaaaaaaat
      361 aacagggctt cttaccacca gtactcctgg cataatgtct ggggtttata aggtattaag
      421 gacaccaggc gcccatcgcg aaagcgacag catccgccgc gtgggt
//
LOCUS       DAXDSD010000240          465 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXDSD010000240 DAXDSD010000000
VERSION     DAXDSD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.244"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>465
                     /locus_tag="ACFB2Q_002215"
     CDS             <1..>465
                     /locus_tag="ACFB2Q_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HFC0919145.1"
                     /translation="SNLIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIK
                     NRLHSTEEDFIRNIHQDLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKD
                     TASVLYAQLTEKHFKTANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKS"
BASE COUNT          197 a          104 c           77 g           87 t
ORIGIN      
        1 tcaaacctga tagcagatta cataaaaagg catcaagata cattgatacc gtatcagata
       61 cccaaaaaca aagcactgca aaaactgatt aatcttaaaa accaattaca gcaacagcag
      121 aagcaaatta aaaaccgtct tcatagcact gaagaagact tcataaggaa catacatcaa
      181 gacttgatag ataccataca ggacaagatg gaacaggtaa aaatagccat atccgaacaa
      241 atcaaaaaac aaacggacaa taaccattac cgcaatcttc aaaccatccc gagcataggc
      301 aaagacaccg catcagttct ttatgcgcaa ctgacagaaa aacattttaa aaccgcaaac
      361 caatttgtat cctatgccgg attaagtccc gccatcatac aatcagggac aagcgtaaga
      421 ggtcggggca gattgagccg atacggaaac agacgattaa aaagt
//
LOCUS       DAXDSD010000241          444 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXDSD010000241 DAXDSD010000000
VERSION     DAXDSD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.245"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..138
                     /locus_tag="ACFB2Q_002216"
     CDS             <1..138
                     /locus_tag="ACFB2Q_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC0919146.1"
                     /translation="EGPFADFNGVVEEVNYERNKLRVSVQIFGRETPVELEFSQVEKI
                     N"
     gene            239..>444
                     /gene="rplK"
                     /locus_tag="ACFB2Q_002217"
     CDS             239..>444
                     /gene="rplK"
                     /locus_tag="ACFB2Q_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395730.1"
                     /note="binds directly to 23S ribosomal RNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC0919147.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFT"
BASE COUNT          131 a           77 c          108 g          128 t
ORIGIN      
        1 gaaggaccgt ttgcggattt taacggggtg gttgaggagg tcaattatga acggaataag
       61 ttacgcgtgt ctgttcagat atttggtaga gaaacacccg ttgagctgga gttcagccag
      121 gttgagaaga ttaactgatt tttatacttg aaaaaaaagc aataagagga tagaatcaaa
      181 aattaacttg gggagcggaa atggttccgc gtcttacccg tttttaggag tccgttaagt
      241 ggcaaagaaa attatcggct atattaaact gcaaattcct gcaggcaaag ccaatccatc
      301 ccccccagtt ggtcctgctt tgggtcaacg tggtttgaat atcatggaat tctgtaaagc
      361 atttaatgct gcaacccaag gtatggaatc tggtttgcca attccggttg tgattactgc
      421 atttgcagat aaatcattca catt
//
LOCUS       DAXDSD010000242          440 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXDSD010000242 DAXDSD010000000
VERSION     DAXDSD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.246"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..408
                     /locus_tag="ACFB2Q_002218"
     CDS             <1..408
                     /locus_tag="ACFB2Q_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC0919148.1"
                     /translation="SAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRV
                     LQKAIPGAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALA
                     SDRPVPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT           94 a          147 c          126 g           73 t
ORIGIN      
        1 tccgccgccg tgtgcttcga tgccgtccaa gccgcgaaag cgcgcgggat cgacatcgtg
       61 cttgccgaca ccgccggccg cctgcccacg cagcttcatt tgatggaaga aatcaaaaaa
      121 gtgaagcgcg tgctgcaaaa agccattccc ggcgcgccgc acgaaatcat cgtcgtactc
      181 gatgccaata tcgggcaaaa cgccgtcaac caagtcaaag cctttgacga cgcattgggg
      241 ctgacggggc ttatcgttac caaactcgac ggcacggcaa aaggcggcat cctcgccgcg
      301 cttgcttccg accgccccgt ccccgtccgc tacatcggcg tgggcgaagg catagacgac
      361 ctgcgcccgt ttgacgcgcg cgcgtttgtg gacgcactgc tggattgagc cgaaatgccg
      421 tccgaaaacg gcagaccgcc
//
LOCUS       DAXDSD010000243          413 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXDSD010000243 DAXDSD010000000
VERSION     DAXDSD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.247"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(52..>413)
                     /locus_tag="ACFB2Q_002219"
     CDS             complement(52..>413)
                     /locus_tag="ACFB2Q_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC0919149.1"
                     /translation="IMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGI
                     NGETVLLADPSLGHVSTSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNP
                     VRQTRFTVEQIQMRQKR"
BASE COUNT          117 a           83 c           74 g          139 t
ORIGIN      
        1 cgccgccttg tcctgattac tatataaaat aaatgggcaa aaatcggttt attatcgttt
       61 ttgccgcatt tggatttgtt ctaccgtaaa acgtgtttga cgaacgggat ttttattaaa
      121 aaacatctga tttctaacaa aatcagtatt ttttggcacg atggctaaaa tttttccttc
      181 catttcgcca tcacgtgttt tccatgcgct caagaattgt gatttgctcg ttgagacgtg
      241 ccccagcgat ggatcagcca gcaaaacagt ttctccgtta ataccgttca ataccgaaaa
      301 atggttgttt ttacggtatt ttaaatacac aattacagga atttttagtt gtaccaactg
      361 ttcaaatggc aaagcataac cttgtgcttc aaaacccagt tcgggcatta tgc
//
LOCUS       DAXDSD010000244          412 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXDSD010000244 DAXDSD010000000
VERSION     DAXDSD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.248"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(1..317)
                     /locus_tag="ACFB2Q_002220"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACFB2Q_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           80 a          120 c          102 g          110 t
ORIGIN      
        1 gccttacgca ccgcagacct tccgccgaag cagcggcttt tgaatttggt ttccccgctc
       61 tccctcgggt gcggggcaat gccgaccaaa cccgctatcc gtttgtgcga cagccgcccc
      121 aattcgggca gcatcgccat cagcgtagcc gtcgttatcg aaccgatgcc tttgatttgc
      181 tccgccactt gggctttgcc gtcaaaatgc gtgcgggtgt ggttgtcgat ttgtttgtcc
      241 gattcgtcaa tcagccggtc aaaacgggca atcagttgtt tgacgctttc gacttgcgtt
      301 tcgtgaacct gatgcagaca ttttgtaata gctgccatta cgcctactag gatatatacc
      361 ttctatatat agtgaattaa caaaaaccgg tacggcgttg ccccgccccg gc
//
LOCUS       DAXDSD010000245          411 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXDSD010000245 DAXDSD010000000
VERSION     DAXDSD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.249"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>411
                     /locus_tag="ACFB2Q_002221"
     CDS             <1..>411
                     /locus_tag="ACFB2Q_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC0919150.1"
                     /translation="ESNSSTKKVTEDIKDNYRETKTEHQGNGSFHAASSLGLSAVYDF
                     KLNDKFKPYIGARVGYGHVRHQVRSVGQETITVTPKPKNGTQGGSVISTSPVPAYHEN
                     RSSRRLGFGAMAGVGIDVAPNLTLDAGYRYRYWGR"
BASE COUNT          114 a          118 c           95 g           84 t
ORIGIN      
        1 gaaagtaatt cttctactaa aaaagttact gaagatataa aagacaacta ccgagaaacc
       61 aaaacagaac atcaaggaaa cggcagcttc cacgccgctt cttctctcgg cttatccgcc
      121 gtttacgatt tcaaactcaa cgataaattc aaaccctata tcggtgcgcg cgtcggctac
      181 ggacacgtca gacatcaggt tcgttcggtt gggcaagaaa ctataactgt tacccctaaa
      241 ccaaagaatg gtacgcaagg aggttctgtt atatcaacta gccccgtccc tgcctatcac
      301 gaaaaccgca gcagccgccg cttgggcttc ggcgcgatgg cgggcgtggg catagacgtc
      361 gcgcccaacc tgaccctgga cgccggctac cgctaccgct attggggacg c
//
LOCUS       DAXDSD010000246          403 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXDSD010000246 DAXDSD010000000
VERSION     DAXDSD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.250"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            107..322
                     /gene="rpmE"
                     /locus_tag="ACFB2Q_002222"
     CDS             107..322
                     /gene="rpmE"
                     /locus_tag="ACFB2Q_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFC0919151.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT          123 a          105 c           75 g          100 t
ORIGIN      
        1 ttttatcgaa tatcctcttg acgaaacaac agaaaatagc gagaatgccg agcttgtctg
       61 aggcataggt acatccccta tgcccgcttt tttgaaagga aatcaaatga aacaaggtat
      121 tcacccgaac taccgcgaag ttaacgttac ctgctcttgc ggcaacaaat tcgtaaccaa
      181 atccgcaatg gaaaaagaaa acttcaacat cgaggtttgc tccctgtgcc acccgttcta
      241 taccggcacc caaaaaatcg tcgataccac cggccgcgtg gacaaattca acaacaaatt
      301 cggcaacctg ttcaaacgct gattgccgct ttgcaagaat taagccctgc catcatttcg
      361 atggcagggc tttttaatat ttggcacact gacttcttat cta
//
LOCUS       DAXDSD010000247          401 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXDSD010000247 DAXDSD010000000
VERSION     DAXDSD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.251"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            68..>401
                     /locus_tag="ACFB2Q_002223"
     CDS             68..>401
                     /locus_tag="ACFB2Q_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC0919152.1"
                     /translation="MSARLMGMAFKTGIPRGQRFVLVKLCDCANDDGLCYPSQETLAE
                     DTGFAETAVRQHIKWLKDNNFIKSARRQRGRERKSDIYRINVALLEKCYAEAAKRKAA
                     RQAKMWEEP"
BASE COUNT          102 a           90 c          137 g           72 t
ORIGIN      
        1 gcatgaaggc gggcggccgc gggaagcctg ttacgcatta ttcgattgtc aaaaacggag
       61 cggaagtatg agtgcgaggc tgatggggat ggctttcaaa acgggtatcc cgagggggca
      121 gcgttttgtt ttggtaaagt tgtgcgactg cgccaacgac gatggcttgt gttatccgtc
      181 gcaagaaacg ctggcggaag atacgggctt cgccgaaacc gccgtacggc agcatatcaa
      241 gtggctgaag gataacaatt tcatcaagtc cgcccggcgg cagagggggc gggagaggaa
      301 gtccgacatc taccgcatca acgtcgccct gcttgaaaaa tgctatgcgg aggcggcaaa
      361 acggaaggcg gcgcggcagg caaaaatgtg ggaagaacca t
//
LOCUS       DAXDSD010000248          392 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXDSD010000248 DAXDSD010000000
VERSION     DAXDSD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.252"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(11..>392)
                     /locus_tag="ACFB2Q_002224"
     CDS             complement(11..>392)
                     /locus_tag="ACFB2Q_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC0919153.1"
                     /translation="PEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNGYKF
                     RRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWNHEI
                     LQQTNDVLAEILRVLQELEKQPAR"
BASE COUNT           72 a          112 c          101 g          107 t
ORIGIN      
        1 caaccgtttg ctaccgtgct ggctgctttt ccaattcctg caatacgcgc aggatttccg
       61 ccagtacatc gtttgtctgc tgcaaaattt cgtgattcca aaaacgcagc acggtaaagc
      121 ccaagctgtt gagatatacc gtccgcgcgt ggtcgtatac ggcttgttcc gcgtgctgcc
      181 cgccgtctgc ttcgacaatc agcttgggcg ttacgcacat aaaatcaaca atataattcc
      241 ccatcggctg ctgtcggcgg aatttatagc cgttcagacg gcctgcccgc aggtgttgcc
      301 acaattttgc ttccgcctcg ctcatttctt gacgcatggc tttggcgcgt tggcgcaggg
      361 cggggttttc ggcggtcaat agtttctcgg gc
//
LOCUS       DAXDSD010000249          392 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXDSD010000249 DAXDSD010000000
VERSION     DAXDSD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.253"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(49..392)
                     /locus_tag="ACFB2Q_002225"
                     /pseudo
     CDS             complement(49..392)
                     /locus_tag="ACFB2Q_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          131 a           78 c           70 g          113 t
ORIGIN      
        1 ccgaccacgc aagccgccga acaaaaaata aggggctgtc ctagataact aggataaact
       61 cgattttact aattgtttta aaatggaaca agaactttta tctcactgtt gttaaaacgc
      121 cattcgcact cctttaaata cagctcaaaa tgcgctttgg gaatgccgtt aaacttgcgt
      181 aaatgacgtt ttgcctgatt ccaaaagttc tcaattccat taatatggtt ttgtcgttcg
      241 gcaaaatgtg tgctgtgatt gatacgaaaa cgaagtttca gcgaagctaa aatggctaaa
      301 ttcgctcaca tctaatacat catagctacg ataacaatcc gtataaacaa tactgtcagg
      361 tttcacttgt tcacggataa taggaaataa ag
//
LOCUS       DAXDSD010000250          392 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXDSD010000250 DAXDSD010000000
VERSION     DAXDSD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.254"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           93 a          115 c          120 g           64 t
ORIGIN      
        1 ccgctgtacc atggataagc gcgcaagggt agaatgatgc ggcaacccta tacattgcac
       61 cccgtcagag gggcgcgtta cctttgcgaa catccccctt tggcagccgg gcgaaggggg
      121 ctttgcaacc ggcaatccgg cgggcgcggg atcgggcggt ttgcccaatc ccgccgtttg
      181 cggcgcgcct gccgccgacg gtatcccgcg aagcaagatt taagggataa aatatgttcc
      241 aacacgcagg gcggcacata aggcgccgcc ctgattcgga agggcttgca cccctcccgg
      301 acaaagcctg atcctgccgc cccgaaggac ggatgcccga agggcggggg gtttcaaccg
      361 aaaaggaaat acgatgaata aaactttaaa aa
//
LOCUS       DAXDSD010000251          390 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXDSD010000251 DAXDSD010000000
VERSION     DAXDSD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.255"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT          130 a           89 c           64 g          107 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaggtctt ttctaaaata tgcagatatt cgaatgtcga
       61 atttgcctta tgattacggt tttctgtctt atccgcctta aaacgccggt attccgtttg
      121 aaccaaatta tatttgccga aacactcaaa aatctccctt acctgcccga cagacatcaa
      181 gacttcgttg ttgtaactga gaaaaatata ttgaaagtcc gcatccctaa tcaaagcctc
      241 aaataccgga accacatccg attttgagca agtccgcgct aaaacacatc gtacagcgca
      301 aaaccggatg ggaatggcaa aatccggaaa caaacatttt tcaaagcgta caaacaaaaa
      361 ataccaaccg gaggttggta tttcttaact
//
LOCUS       DAXDSD010000252          386 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXDSD010000252 DAXDSD010000000
VERSION     DAXDSD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.256"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>386
                     /locus_tag="ACFB2Q_002226"
     CDS             <1..>386
                     /locus_tag="ACFB2Q_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC0919154.1"
                     /translation="RNKDNGNRTDLKAENQENGTFHAVSSLGLSAVYDFKLNDKFKPY
                     IGARVAYGHVRHSIDSTKKTAEVTTILHDPGTTPTVYPGKNTQDAHRESDSIRRVGLG
                     AVAGVGIDITPNLTLDAGYRYHNWGR"
BASE COUNT          108 a          120 c           94 g           64 t
ORIGIN      
        1 taagaaacaa ggacaatggc aacaggacag acctgaaggc ggaaaatcag gaaaacggta
       61 cgttccacgc cgtctcctcg ctcggtttgt cagccgttta cgatttcaaa ctcaacgata
      121 aattcaaacc ctatatcggt gcgcgcgtcg cctacggaca cgtcagacac agcatcgatt
      181 cgaccaagaa aacagcagag gttactacca tcctccatga tcctggcaca acccctacgg
      241 tttatcctgg gaaaaatacg caagacgccc atcgcgaaag cgacagcatc cgccgcgtgg
      301 gcctcggcgc agtggcaggc gtaggcatcg acatcacgcc caacctgacc ttggacgccg
      361 gctaccgcta ccacaactgg ggacgc
//
LOCUS       DAXDSD010000253          372 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXDSD010000253 DAXDSD010000000
VERSION     DAXDSD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.257"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           90 a           95 c           98 g           89 t
ORIGIN      
        1 agggcggtgc tttatgtgtc gtcctgtgtg ttgaaacata tcggacgcgt ttgaacagtt
       61 gtgcggcaaa ctgttttagc ggaaggcaag agatgccgtc tgaagcccat tgtccgtcat
      121 tcccacacag gcagttgcta cggttgctga agccccttag ccgtcgcgca ggcagttgtt
      181 acggttacta aaatcccgtc atttcctcaa aaacagaaaa ccaaaatcag aaacctaaaa
      241 tcccgtcatt cccgcgcagg cgggaatccg ggcctgtcgg cacggaaact tatcggataa
      301 aacggtttct ttagatttta cgttccggat tcccgcctgc gcgggaatga cggcgggaga
      361 acaatgccgt ct
//
LOCUS       DAXDSD010000254          365 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXDSD010000254 DAXDSD010000000
VERSION     DAXDSD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.258"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..>365)
                     /locus_tag="ACFB2Q_002227"
     CDS             complement(<1..>365)
                     /locus_tag="ACFB2Q_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919155.1"
                     /translation="REASQAVAQWTKDFDDLSPNQRQLVLRPLSNLFAAYEEFLKDAP
                     ARLIAEVSAYSLAVRVAKKAMAFLELDGGLISAVGKVVNGADSRAEARRLKMPYAEFT
                     GRILHAANLLYDVGIQADK"
BASE COUNT           72 a          110 c          104 g           79 t
ORIGIN      
        1 ccttgtccgc ctgaatgccc acatcgtaaa gcaggttcgc ggcgtgtagg atccggcctg
       61 taaattccgc gtacggcatt ttcaggcggc gggcttccgc gcgcgaatcc gcgccgttga
      121 cgactttgcc gaccgccgaa atcaaaccgc cgtcaagttc taaaaacgcc atggctttct
      181 tggcaacccg cacggccaat gagtatgccg atacttcggc tatcagccgt gcaggcgcgt
      241 ctttcagaaa ttcttcgtac gcggcgaaga gatttgacag cggacgcagc acgagttggc
      301 gctgattcgg gctaaggtcg tcgaaatcct tagtccattg cgcgaccgcc tgcgatgctt
      361 cgcgg
//
LOCUS       DAXDSD010000255          362 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXDSD010000255 DAXDSD010000000
VERSION     DAXDSD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.259"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>362
                     /locus_tag="ACFB2Q_002228"
     CDS             <1..>362
                     /locus_tag="ACFB2Q_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC0919156.1"
                     /translation="LLSESGRTQTVRNIIVKPTPLKAYNLTVADWHTYFVKGNQAETE
                     GVWVHNACPPRKTPSTPVYGNDSEAYAAAKKLGYRKIKERTRNDAAIFKKGKSYISRD
                     VDSHNGGAWKEASSPKNL"
BASE COUNT          130 a           77 c           81 g           74 t
ORIGIN      
        1 gactgttatc cgaaagcggc agaacccaaa ccgtccgcaa catcattgtc aaaccaacac
       61 cgctcaaagc ctacaatctg accgttgccg actggcatac ctacttcgtc aagggtaatc
      121 aggcggaaac ggaaggggtt tgggttcata atgcgtgtcc gccaagaaaa acaccatcta
      181 cccccgtata tgggaatgat tcagaagcat atgcagcagc aaagaaattg ggatatagaa
      241 aaataaaaga aagaacgaga aatgatgctg ctatttttaa aaaaggaaag agttatatat
      301 cacgcgatgt agattcacat aatggagggg cttggaaaga agcatcttca cccaaaaatc
      361 tg
//
LOCUS       DAXDSD010000256          356 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXDSD010000256 DAXDSD010000000
VERSION     DAXDSD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.260"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..276
                     /locus_tag="ACFB2Q_002229"
     CDS             <1..276
                     /locus_tag="ACFB2Q_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC0919157.1"
                     /translation="KYQPSSEYFGQSMAFIAQKEFEEFVNNVKEYDILESFSYFLNKR
                     VAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
BASE COUNT          127 a           42 c           60 g          127 t
ORIGIN      
        1 aaatatcagc cgtcaagcga gtattttggg caatcgatgg cttttattgc tcagaaagaa
       61 tttgaagaat ttgttaataa cgtaaaagag tacgatattt tagagagctt ctcttatttc
      121 ttaaataaga gggtcgcaca taatatttgg aagatttatt tttctgatga gtctgttatt
      181 tttataagaa aatcagaaga gaacggaaaa actgttcatg aattcgttta tcaagagtac
      241 accgatagtt ctgattttaa ttctatgttc gagtaattct tattctgaac ctgtaagatt
      301 agaaaaatca caaattaaat ttcaatcaag taataattta aaatcaattg gtttta
//
LOCUS       DAXDSD010000257          355 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXDSD010000257 DAXDSD010000000
VERSION     DAXDSD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.261"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            1..355
                     /locus_tag="ACFB2Q_002230"
                     /pseudo
     CDS             1..355
                     /locus_tag="ACFB2Q_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
BASE COUNT          107 a          108 c           88 g           52 t
ORIGIN      
        1 ccctcaccga atattacctg aaaacggcga atggcccaaa gacaacggcg ctgccggcgt
       61 ggcatccgct tcaacaatca aaggcaaata tgttcagaaa gttgaagtcg caaaaggcgt
      121 cgtcaccgcc caaatggctt caaccggcgt aaacaaagaa atcaaaggca aaagactctc
      181 cctgtgggcc aagcgtcaag acggttcggt aaaatggttc tgcggacagc cggttaagcg
      241 cgacaacgcc gacgacccca acgacgccgt caaagacgac gccgacgcca acggcaaaat
      301 cagcaccaag cacctgccgt caacctgccg tgatacgtca tctgccggta cgtga
//
LOCUS       DAXDSD010000258          349 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXDSD010000258 DAXDSD010000000
VERSION     DAXDSD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.262"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>349
                     /locus_tag="ACFB2Q_002231"
     CDS             <1..>349
                     /locus_tag="ACFB2Q_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFC0919158.1"
                     /translation="DRPLYFCHIDGLDKRKFNARELAEGQIMSAPLRDVIPEGAVLIV
                     GEAHYTYPVRAAGRPVPPYIQELTELRHHGHTVILMTRHPSQLDIFVRNLVSKHVHLE
                     RKAIGMKQYYWYK"
BASE COUNT           87 a           99 c           86 g           77 t
ORIGIN      
        1 cagaccggcc gctttatttc tgccatatcg acggattgga caaacgaaaa ttcaatgccc
       61 gcgaactggc ggaagggcaa atcatgtccg ccccgcttcg tgatgtcata ccggaaggcg
      121 cggtgctgat tgttggcgaa gcgcactaca cttacccggt acgcgcggca ggccgtcccg
      181 ttccgcccta tattcaggaa ctgacagaac tccgccatca cgggcatacc gtcattttga
      241 tgacgcggca cccgagccaa cttgatatat tcgtccgcaa ccttgtttca aagcatgtac
      301 accttgaacg caaggcaatc ggcatgaaac agtattattg gtataaatg
//
LOCUS       DAXDSD010000259          349 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXDSD010000259 DAXDSD010000000
VERSION     DAXDSD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.263"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>349
                     /locus_tag="ACFB2Q_002232"
     CDS             <1..>349
                     /locus_tag="ACFB2Q_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC0919159.1"
                     /translation="GGWRIAADYARYRKWNNSKYSVNTKNLRTHESNGNRQDLKTENQ
                     ENGTFHAVSSLGLSAVYDFDTGSRFKPYAGVRVAYGHVKHQVHSVEKETTLTILAPSK
                     GATEPGKIILGPI"
BASE COUNT          103 a           93 c           81 g           72 t
ORIGIN      
        1 tcggcggctg gcgcatcgcc gcggattatg cccgttacag aaaatggaac aacagtaaat
       61 attccgtcaa cacaaaaaat ttgcggacac atgaaagcaa tggcaacagg caagatctga
      121 agacggaaaa tcaggaaaac ggtacgttcc acgccgtttc ttctctcggc ttgtccgccg
      181 tttacgattt cgataccggt tcccgcttca aaccctatgc aggcgtgcgc gtcgcctacg
      241 gacacgttaa acatcaggtt cattcagtgg aaaaagaaac tacgcttaca atccttgcac
      301 catcgaaagg cgctacagag ccaggcaaga tcatactagg tccgatcag
//
LOCUS       DAXDSD010000260          346 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXDSD010000260 DAXDSD010000000
VERSION     DAXDSD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.264"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>346
                     /locus_tag="ACFB2Q_002233"
     CDS             <1..>346
                     /locus_tag="ACFB2Q_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC0919160.1"
                     /translation="RNDNSTSSSNHLNIKTQKTEHQENGTFHAASSLGLSTIYDFDTG
                     SRFKPYIGARVAYGHVRHQVRSVEQETVAVTTYPKEQNVAPSVTTEAPTKKPAYHENR
                     SSRRLGFGAMAGV"
BASE COUNT          101 a          105 c           73 g           67 t
ORIGIN      
        1 aaagaaacga taatagcact tctagcagca accatcttaa cataaaaacc caaaagacgg
       61 aacatcaaga aaacggcaca ttccacgccg cctcttctct cggcttgtca accatttacg
      121 atttcgatac cggttcccgc ttcaaaccct atatcggcgc acgcgtcgcc tacggacacg
      181 tcagacatca ggttcgttcg gttgaacaag aaaccgttgc tgttaccact tacccaaagg
      241 aacagaatgt tgcgccaagt gttaccacag aggctccgac caaaaaacct gcctatcacg
      301 aaaaccgcag cagccgccgc ttgggcttcg gcgcgatggc gggcgt
//
LOCUS       DAXDSD010000261          346 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXDSD010000261 DAXDSD010000000
VERSION     DAXDSD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.265"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>346
                     /locus_tag="ACFB2Q_002234"
     CDS             <1..>346
                     /locus_tag="ACFB2Q_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HFC0919161.1"
                     /translation="VELSGQKHVFMFGAMGLGGRGAYALDLSKINENYPAAAPLFDVK
                     NGDNNGKNRVEVELGYTVGTPQIGKIRNGKYAAFLASGYAAKDIDSQENKTALYVYDL
                     KDTLGTPITKIDV"
BASE COUNT          104 a           80 c           88 g           74 t
ORIGIN      
        1 aagtcgaact gagcgggcaa aaacacgtgt ttatgttcgg cgcgatgggt ttgggcggca
       61 gaggcgcgta tgccttggat ttaagcaaaa tcaacgaaaa ttatccggcc gccgctcccc
      121 tgtttgatgt caaaaatggc gataataacg gcaaaaatcg cgtggaagtg gaattaggct
      181 acaccgtcgg tacgccgcaa atcggcaaaa tccgcaacgg caaatacgcc gccttcctcg
      241 cctccggtta tgcggctaaa gatattgaca gccaagaaaa taaaaccgcg ctgtatgtat
      301 atgatttgaa agacacctta ggtacgccga ttacaaaaat cgatgt
//
LOCUS       DAXDSD010000262          344 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXDSD010000262 DAXDSD010000000
VERSION     DAXDSD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.266"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>344
                     /locus_tag="ACFB2Q_002235"
     CDS             <1..>344
                     /locus_tag="ACFB2Q_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC0919162.1"
                     /translation="NNKTSDGDQLNIKYQKTEHQENGTFHAVSSLGLSTVYDFRVNDK
                     FKPYIGVRVGYGHVRHGIDSTKKTKNTLTAYHGAGTKPTYYDDIDSGKNQKNTYRQNR
                     SSRRLGFGAMAGV"
BASE COUNT          112 a           88 c           71 g           73 t
ORIGIN      
        1 aataataaaa cttctgacgg cgaccagctt aacataaaat accaaaagac ggaacatcag
       61 gaaaacggca cattccacgc cgtttcttct ctcggcttgt caaccgttta cgatttcaga
      121 gtcaacgata aattcaaacc ctatatcggt gtgcgtgtcg gctacggaca cgtcagacac
      181 ggtatcgatt cgactaaaaa aacgaaaaat actcttaccg cctaccatgg tgctggcaca
      241 aaacctacgt attatgatga tatagattcg ggaaaaaacc aaaaaaacac ttatcgccaa
      301 aaccgcagca gccgccgctt gggcttcggc gcgatggcgg gcgt
//
LOCUS       DAXDSD010000263          340 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXDSD010000263 DAXDSD010000000
VERSION     DAXDSD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.267"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..>340)
                     /locus_tag="ACFB2Q_002236"
     CDS             complement(<1..>340)
                     /locus_tag="ACFB2Q_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919163.1"
                     /translation="YMAGGVYLGGKIISPIYHSSQEFSGEPIIYAETNIILCPAEKFL
                     TLKRVFHNGNIFNMNQIITFLSNKQGGVRFDKNYDKYKTWQVAIEKAANFLKLGNPYN
                     EDKLSLSEEHD"
BASE COUNT          115 a           51 c           42 g          132 t
ORIGIN      
        1 tgtcatgttc ttcactaaga gataatttat cttcattata tggattaccg agttttaaaa
       61 aatttgctgc tttttctata gcaacttgcc aagttttata tttatcgtaa tttttatcaa
      121 aacgtactcc cccttgttta ttggataaaa aagtaattat ctgattcata ttaaaaatat
      181 ttccattatg gaaaactcgt tttaaagtta aaaatttttc agcaggacat aaaattatat
      241 ttgtttcagc atagattatt ggttcaccag aaaattcttg tgatgaatga tatatagggc
      301 taataatttt tccgcctaaa taaactccac cagccatata
//
LOCUS       DAXDSD010000264          333 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXDSD010000264 DAXDSD010000000
VERSION     DAXDSD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.268"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>333
                     /locus_tag="ACFB2Q_002237"
     CDS             <1..>333
                     /locus_tag="ACFB2Q_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC0919164.1"
                     /translation="KWDWVKNTGYEKPAARPMQTVDGEMAGGNKPIKSLPNSAAEKRK
                     QSFKKFSSNWSSASFDSVHKTLTPNAPGILSPDKVKTRYTSLDGKITIIKDNENNYFR
                     IHDNSRKQ"
BASE COUNT          130 a           59 c           62 g           82 t
ORIGIN      
        1 ccaaatggga ttgggttaaa aataccggct atgaaaaacc tgctgcccga cctatgcaga
       61 ctgttgacgg ggaaatggca ggtgggaata aacctattaa atctttacca aacagcgccg
      121 ctgaaaaaag aaaacaaagt tttaagaagt ttagtagcaa ctggagttca gcaagttttg
      181 attcagtgca caaaacacta actcccaatg cacctggtat tttaagtcct gataaagtta
      241 aaactcgata cactagttta gatggaaaaa ttacaattat aaaagataac gaaaacaact
      301 attttagaat ccatgataat tcacgaaaac agt
//
LOCUS       DAXDSD010000265          321 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXDSD010000265 DAXDSD010000000
VERSION     DAXDSD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.269"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>321
                     /locus_tag="ACFB2Q_002238"
     CDS             <1..>321
                     /locus_tag="ACFB2Q_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC0919165.1"
                     /translation="NRGNRKERKTENQENGTFHAASSLGLSAVYDFKLNDKFKPYIGA
                     RVAYGHVRHQVHSMEKETTTVFTVPTGGAPKGGVIPETTISKLPHHESRSISSLGFGA
                     VAGVG"
BASE COUNT           93 a           93 c           80 g           55 t
ORIGIN      
        1 aaccggggca acaggaaaga acggaagacg gaaaatcagg aaaacggtac gttccacgcc
       61 gcctcctcgc tcggcttgtc cgccgtttac gatttcaaac tcaacgacaa attcaaaccc
      121 tatatcggcg cacgcgtcgc ctacggacac gtcagacatc aggttcattc aatggaaaaa
      181 gaaaccacga ctgttttcac tgtaccaacg ggaggcgctc caaagggagg tgttatccca
      241 gaaactacga tcagcaaact tccccatcac gaaagccgca gcatcagcag cttgggcttc
      301 ggcgcagtgg caggcgtagg c
//
LOCUS       DAXDSD010000266          318 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXDSD010000266 DAXDSD010000000
VERSION     DAXDSD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.270"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>318
                     /locus_tag="ACFB2Q_002239"
     CDS             <1..>318
                     /locus_tag="ACFB2Q_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFC0919166.1"
                     /translation="VARCGLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQ
                     ELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPAVLAKLKDQASVGAPAPK
                     GPAK"
BASE COUNT           72 a           84 c           98 g           64 t
ORIGIN      
        1 gtcgcaagat gcggtttgtt gtttgcaacc cttaaaggaa aaaccatgaa aaaaatgttt
       61 gtattgttct gtatgctgtt ctcctgcgcc ttctcccttg cggcggtaaa catcaatgcg
      121 gcttcgcagc aggagctgga ggcgctgccg ggcataggcc cggcgaaggc gaaggccatt
      181 gcggaatacc gcgcgcaaaa cggcgcgttc aagtctgtgg acgatttgat caaggtgaag
      241 ggcatcggtc cggcggtgct ggcgaagctg aaagaccagg cttccgtcgg cgcgcccgca
      301 ccaaaaggcc ccgccaaa
//
LOCUS       DAXDSD010000267          317 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXDSD010000267 DAXDSD010000000
VERSION     DAXDSD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.271"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            106..>317
                     /locus_tag="ACFB2Q_002240"
     CDS             106..>317
                     /locus_tag="ACFB2Q_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC0919167.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMPVWYELHPTMESAITREKQLKKW"
BASE COUNT          102 a           47 c           82 g           86 t
ORIGIN      
        1 agagttgcgg gaatgatgga aagctatggg gataacgaag ggttaaagta atcaccggat
       61 ggtgttcgcg ggaatataaa tttaaataat tcaaaagggt attatatgca gcctgcggtt
      121 tatattttag caagccaacg taatggcacg ttatacattg gcgttacatc tgatttggtg
      181 caacgtattt accaacatag ggagcatttg attgaaggat ttacatcacg gtacaacgtt
      241 actatgccgg tttggtatga actgcatcct acgatggaga gcgcaattac tcgggaaaaa
      301 cagttgaaga aatggaa
//
LOCUS       DAXDSD010000268          317 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXDSD010000268 DAXDSD010000000
VERSION     DAXDSD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.272"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>317
                     /locus_tag="ACFB2Q_002241"
     CDS             <1..>317
                     /locus_tag="ACFB2Q_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC0919168.1"
                     /translation="WPKDNASAGVASTPTDIKGKYVQKVEVNNGVVTAQMNPSGVNKE
                     IQGKRLSLWAKRQDGSVKWFCGQPVTRDNAANDDVAKDDAGTNAIETKHLPSTCRDNF
                     DAS"
BASE COUNT           94 a          101 c           77 g           45 t
ORIGIN      
        1 atggccgaaa gacaacgctt ctgccggcgt ggcatccacc cccaccgaca tcaaaggcaa
       61 atatgttcag aaagttgaag tcaacaacgg cgtcgtcacc gcccaaatga atccaagcgg
      121 cgtaaacaaa gaaatccaag gcaaaagact ctccctgtgg gccaagcgtc aagacggttc
      181 ggtaaaatgg ttctgcggac agccggttac gcgcgacaac gccgccaacg acgacgtcgc
      241 caaagacgac gccggcacca acgccatcga aaccaagcac ctgccgtcaa cctgccgcga
      301 taactttgat gccagct
//
LOCUS       DAXDSD010000269          316 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXDSD010000269 DAXDSD010000000
VERSION     DAXDSD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.273"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>316
                     /locus_tag="ACFB2Q_002242"
     CDS             <1..>316
                     /locus_tag="ACFB2Q_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC0919169.1"
                     /translation="KNQNKRDLKTENQENGSFHAVSSLGLSAVYDFKLNDKFKPYIGA
                     RVAYGHVRHSIDSTKKTTRFLTSSYGGAKPTVYPEENTQDAHHQSNSIRRVGLGVIAG
                     VGF"
BASE COUNT           92 a           92 c           68 g           64 t
ORIGIN      
        1 aagaatcaga ataagagaga cctgaagacg gaaaatcagg aaaacggcag cttccacgcc
       61 gtttcttctc tcggcttatc agccgtttac gatttcaaac tcaacgacaa attcaaaccc
      121 tatatcggtg cgcgcgtcgc ctacggacac gtcagacaca gcatcgattc gaccaaaaaa
      181 acaacacggt ttcttacctc ctcctatggt ggcgcaaaac ctacggttta tcctgaggaa
      241 aatacgcaag acgcccatca ccaaagcaac agcatccgcc gcgtaggtct cggtgtcatc
      301 gccggcgtcg gtttcg
//
LOCUS       DAXDSD010000270          314 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXDSD010000270 DAXDSD010000000
VERSION     DAXDSD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.274"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            216..>314
                     /locus_tag="ACFB2Q_002243"
     rRNA            216..>314
                     /locus_tag="ACFB2Q_002243"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           93 a           57 c           84 g           80 t
ORIGIN      
        1 gaaaagtata gcacggaatg tcggaaatat cgagagatat cttagacagg cggaagggat
       61 actttataat ttcgcaacgc tctttaacaa aacagattac cgataagtgt gagtgccttg
      121 ggcctcgcac tgtttgaaag acagacgaga tgatgttttg aacattgtcc tgtcggtttc
      181 tttgaagcag accagaagtt aaaaagttag agattgaaca taagagtttg atcctggctc
      241 agattgaacg ctggcggcat gctttacaca tgcaagtcgg acggcagcac agggaagctt
      301 gcttctcggg tggc
//
LOCUS       DAXDSD010000271          310 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXDSD010000271 DAXDSD010000000
VERSION     DAXDSD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.275"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>310
                     /locus_tag="ACFB2Q_002244"
     CDS             <1..>310
                     /locus_tag="ACFB2Q_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC0919170.1"
                     /translation="WPENNDSAGVASASKIIGKYVKSVTVTNGVVTAQMKPSGVNNEI
                     KDKKLSLWAKRQDGSVKWFCGQPVKRDAGAKTGADDVKADGKDTDKINTKHLPSTCRD
                     K"
BASE COUNT          102 a           90 c           75 g           43 t
ORIGIN      
        1 atggccggaa aacaacgact ctgccggcgt ggcatccgct tcaaaaatca taggcaaata
       61 tgttaaaagc gttacggtca caaacggcgt cgttaccgcc caaatgaaac caagcggcgt
      121 aaacaatgaa atcaaagaca aaaaactctc cctgtgggcc aagcgtcaag acggttcggt
      181 aaaatggttc tgcggacagc cggttaagcg cgacgccggc gccaaaaccg gcgccgacga
      241 cgtcaaagcc gacggcaaag acaccgacaa aatcaacacc aagcacctgc cgtcaacctg
      301 ccgcgataaa
//
LOCUS       DAXDSD010000272          306 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXDSD010000272 DAXDSD010000000
VERSION     DAXDSD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.276"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..202
                     /locus_tag="ACFB2Q_002245"
     CDS             <1..202
                     /locus_tag="ACFB2Q_002245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919171.1"
                     /translation="CRRRHRRARRGRGVMMNPTEFLKARIAEWEAKSKEAGGNADFKA
                     FEFAESEIKNYKAMLKTYERPD"
BASE COUNT           78 a           78 c           94 g           56 t
ORIGIN      
        1 ctgccgccgc cgccaccggc gcgcaaggcg cgggagaggc gtaatgatga atcctaccga
       61 gtttctgaaa gcgcgtatcg ccgaatggga agccaaaagc aaagaggcgg gcggaaacgc
      121 cgattttaaa gcttttgagt ttgccgaaag cgaaatcaaa aactacaaag cgatgttgaa
      181 aacttatgaa cggcctgatt aaaaacccga agctgattgc ggtgtcggtt tgcgccgtgt
      241 tcgttgccgg cgcgtggcaa tacgaccgcg ccgcgcaata ccgtcgcgga tacggtgcgg
      301 ctatgc
//
LOCUS       DAXDSD010000273          304 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXDSD010000273 DAXDSD010000000
VERSION     DAXDSD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.277"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT          107 a           47 c           56 g           94 t
ORIGIN      
        1 agcaagcagc attagatgcc gcaaacatgg ggagaagcta ttcaatttag aattaaaaaa
       61 acaaagtgaa aatggactag caccaccaaa ttggtctacc cagtttccta atggtagtat
      121 ttatgatact aaggtaacga aatgattatt caaaatgaat ttaatttata tcctagtaat
      181 atgcttcctg aagggttttg ttatcctgaa aagtatgttc gtatctctaa cgatacatct
      241 ttaatacctt atattcagcc acataatttt cactggtggt ttgagaatta tggaacagaa
      301 gggg
//
LOCUS       DAXDSD010000274          302 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXDSD010000274 DAXDSD010000000
VERSION     DAXDSD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.278"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           82 a           82 c           63 g           75 t
ORIGIN      
        1 gttctgcctt tcaaaggttg ttttgaaagc ctgattttga caccataact tcatgcgctc
       61 aatccttaaa cagaaccgcc ccgattaata cggggacggc aacgccgaga tagaaataaa
      121 aatccatcat ttcaaaacct ttttcagcag ggaaacaaag taaacggacg cgaggacgcc
      181 gaatactatc cggcctgttt caagaccgct ttgcaggttg tctttcggac tgcattccgc
      241 caatgaaagc cttagcggct gaccgtccga catcttccac atgctgccgt tatattccgg
      301 cc
//
LOCUS       DAXDSD010000275          301 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXDSD010000275 DAXDSD010000000
VERSION     DAXDSD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.279"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..183
                     /locus_tag="ACFB2Q_002246"
     CDS             <1..183
                     /locus_tag="ACFB2Q_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919172.1"
                     /translation="TRSVHPRLALGYDFGGWRVFSAPAVAAGLHQSLQYYRSRHCRRF
                     RQIHQFRRITPIPQKP"
BASE COUNT           58 a          129 c           56 g           58 t
ORIGIN      
        1 acccgctccg tccacccccg actcgccctc ggctacgatt tcggcggctg gcgcgtcttt
       61 tccgcccccg ccgttgccgc cggcctccac caatcccttc aatattaccg atcccgccat
      121 tgccgccgat tccgtcaaat ccatcaattc cgccgaatca cgcctattcc ccaaaaacct
      181 tgatgcggcg ggctgaagcc cgccctgcaa ccctctctat gcaccccctt gcgagcccga
      241 cactacgcaa catcttgaga acccatcctg tcaagaatac ccgaaccgtc ccgatacacc
      301 g
//
LOCUS       DAXDSD010000276          301 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXDSD010000276 DAXDSD010000000
VERSION     DAXDSD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.280"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            103..301
                     /locus_tag="ACFB2Q_002247"
                     /pseudo
     CDS             103..301
                     /locus_tag="ACFB2Q_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815999.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT           89 a           88 c           67 g           57 t
ORIGIN      
        1 acggaaacct gcatcccgtc attcccacaa aagtgggaat ccggaacgaa aaatctcaag
       61 aaaccgtttt cccgataagt ttccgcactg acagacctgg attcccgcct gcgcgggaat
      121 gacgaagcca tccttttggc cgaaggtcaa aaatcagccg ttaccgggta ttacctgaat
      181 cacggcgaat ggcccaaaga caacacttct gccggcgtgg catccgcctc cgacatcaaa
      241 ggcaaatatg ttaaaagcgt tacggtcaca aacggcgtcg ttaccgccca aatgaatcca
      301 a
//
LOCUS       DAXDSD010000277          301 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXDSD010000277 DAXDSD010000000
VERSION     DAXDSD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.281"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT          107 a           85 c           73 g           36 t
ORIGIN      
        1 cccgaaaccg cttataacgg cggcggagaa acagagccgt acggaccaag ccccgaagaa
       61 atcaaatacc ggcaaagccc ggaaaccgcc gaaacacggc ggatgaccga aaaacaggca
      121 gaaggccaca ttaaaagcat tatcaggtaa cgccgccgcg caaggcataa aacagcgcga
      181 gaccgataga cgaaacgacc tttaacagga gaccgaaaat gtcattgatt ttaagtgtga
      241 aagacgaaag caatttcaaa ccatgcccgg caggcagcca tcacgccacc tgcatccgca
      301 t
//
LOCUS       DAXDSD010000278          299 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXDSD010000278 DAXDSD010000000
VERSION     DAXDSD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.282"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>299
                     /locus_tag="ACFB2Q_002248"
     CDS             <1..>299
                     /locus_tag="ACFB2Q_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication initiation protein, NGO0469
                     family"
                     /protein_id="HFC0919173.1"
                     /translation="KRQHKILVQWEIDPEGDPEMLMPDGRPYLISRRYTASLHSKSQL
                     ATDLKSWRGRDFTPEERDNFDLRNILGKPCLLSIAHQESSDGKTTYANISAISNK"
BASE COUNT           88 a           90 c           75 g           46 t
ORIGIN      
        1 aagcggcagc acaagatttt agtgcagtgg gaaatcgacc ctgaaggcga tccggaaatg
       61 ctgatgccgg acggcaggcc ctacctcatc agccgccggt acaccgccag cctgcacagc
      121 aaaagccaac tggcaacaga cctcaaaagc tggcgcggaa gggactttac accggaagaa
      181 cgcgacaact tcgatttgcg caatattttg ggcaagccct gcctgttgag tatcgcccac
      241 caagaaagca gcgacggcaa aaccacctat gccaacattt ccgccatcag caacaagat
//
LOCUS       DAXDSD010000279          290 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXDSD010000279 DAXDSD010000000
VERSION     DAXDSD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.283"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           86 a           60 c           48 g           96 t
ORIGIN      
        1 acggcgtttt gacaacagtg gggtaaaagt tcgaatttcc attttaaaac aattggtaaa
       61 gcaggattta tcccggttgg ccgggatttt aaaaaataaa tacattcttt aacaaaacaa
      121 atcatatccc ggcaagacaa atctgataaa atgcttggcg tttattaaca atctttttaa
      181 taatttaatc ttagatattc ggagttgata tgcatacggc cgacaaaaaa cttctcttct
      241 cttctcttct cttctcttct cttctcttct cttccgcagc gcaggcggcg
//
LOCUS       DAXDSD010000280          289 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXDSD010000280 DAXDSD010000000
VERSION     DAXDSD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.284"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           71 a           78 c           81 g           59 t
ORIGIN      
        1 gcgggctata gggtaggctt tatcccgcca atctcactga atccgtcaat ttccgcaatt
       61 caattaaata ccgtcaaacc gatgccgtca ttcccgcgca ggcgggaatc cggaccggtc
      121 gggcatctgc ggcggtttgc taaaaaaacg ctttaccgtg ataagtgcgc aaagttaaaa
      181 tggggaggta agcttttcaa tcagcaatcc ggcgggcgcg gaatcgggcg gtttaccgaa
      241 ccccggcgtt cgcggcgcgc cgtcccgcga aggcaaactt aaggaataa
//
LOCUS       DAXDSD010000281          287 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXDSD010000281 DAXDSD010000000
VERSION     DAXDSD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.285"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..>287)
                     /gene="pglE"
                     /locus_tag="ACFB2Q_002249"
     CDS             complement(<1..>287)
                     /gene="pglE"
                     /locus_tag="ACFB2Q_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(galactosaminyl)2-N,
                     N'-diacetylbacillosaminyl-alpha1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglE"
                     /protein_id="HFC0919174.1"
                     /translation="TKYIYQDNQGLSEARNTGIKNSNGKYIAFIDSDDFINCQVLLDF
                     LGKDDSDMPDVVFLNAVKYDKGRVSYFGEDYQPEKILNQSKVEVLKGLCRF"
BASE COUNT           94 a           62 c           29 g          102 t
ORIGIN      
        1 ctaaatcggc acaatccttt caaaacttcg actttggatt gatttaatat tttttcaggc
       61 tgataatctt cgccaaaata tgaaactctt cccttatcat atttaactgc atttaaaaaa
      121 accacatccg gcatatcact atcatcttta ccaagaaaat ccagcaaaac ctgacaatta
      181 ataaaatcat ccgaatcaat aaatgctata tattttccat ttgaattttt tattccggta
      241 tttctcgcct ccgacaatcc ttggttatcc tgatatatat atttggt
//
LOCUS       DAXDSD010000282          275 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXDSD010000282 DAXDSD010000000
VERSION     DAXDSD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.286"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>275
                     /gene="mafA"
                     /locus_tag="ACFB2Q_002250"
     CDS             <1..>275
                     /gene="mafA"
                     /locus_tag="ACFB2Q_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC0919175.1"
                     /translation="QTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNAET
                     LKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKG"
BASE COUNT           98 a           62 c           54 g           61 t
ORIGIN      
        1 cagaccgtat ttttcctgcg cggcatagac gttgtttctc ctgccaatgc cgatacagat
       61 gtgtttatta acatcgacgt attcggaacg atacgcaaca gaaccgaaat gcacctatac
      121 aatgccgaaa cactgaaagc ccaaacaaaa ctggaatatt tcgcagtaga cagaaccaat
      181 aaaaaattgc tcatcaaacc caaaaccaat gcgtttgaag ctgcctataa agaaaattac
      241 gcattgtgga tggggccgta taaagtaagc aaagg
//
LOCUS       DAXDSD010000283          275 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXDSD010000283 DAXDSD010000000
VERSION     DAXDSD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.287"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(11..>275)
                     /locus_tag="ACFB2Q_002251"
     CDS             complement(11..>275)
                     /locus_tag="ACFB2Q_002251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919176.1"
                     /translation="FDENGRHVGLMEMAGRSTPYGPPDFRLRGNDGVSGCCFRLLFLS
                     FCFGLLFYRNDEILDCKNLSPPPSFPRKWESRNKKQQEFIGNN"
BASE COUNT           74 a           72 c           55 g           74 t
ORIGIN      
        1 ccgttcggtt tcagttattt ccgataaatt cctgctgctt tttatttcta gattcccact
       61 tccgtgggaa tgacggcgga ggggataagt tcttgcaatc taaaatttcg tcatttctat
      121 aaaatagcaa accgaaacag aaacttaaaa acagcaacct gaaacagcaa cctgaaaccc
      181 cgtcattccc gcgcaggcgg aaatccggcg ggccgtaggg tgtgcttcgg cccgccattt
      241 ccatcaatcc aacatgtcta ccgttttcat cgaat
//
LOCUS       DAXDSD010000284          274 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXDSD010000284 DAXDSD010000000
VERSION     DAXDSD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.288"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>274
                     /locus_tag="ACFB2Q_002252"
     CDS             <1..>274
                     /locus_tag="ACFB2Q_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HFC0919177.1"
                     /translation="PGNDDKFKSINREPGKYSQRYRIRENGNRDLGDIVNSPIVAVGE
                     YLATAANDGMVHIFKKNGGSDERSYNLKLSYIPGTMPRKDIESKDST"
BASE COUNT           87 a           69 c           68 g           50 t
ORIGIN      
        1 gtcccggtaa tgatgataaa tttaaatcaa ttaaccgaga accaggcaaa tacagccaaa
       61 gataccgcat ccgcgaaaac ggcaatcgcg atttgggcga catcgtcaac agcccgattg
      121 tcgcggtcgg cgagtatttg gcaaccgccg cgaacgacgg gatggtgcat atcttcaaaa
      181 aaaacggcgg cagtgatgaa cgcagctaca atctgaagct cagctacatc cccggtacga
      241 tgccgcgcaa ggatattgaa agcaaagatt ctac
//
LOCUS       DAXDSD010000285          272 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXDSD010000285 DAXDSD010000000
VERSION     DAXDSD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.289"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           73 a           81 c           53 g           65 t
ORIGIN      
        1 tcctcgcctt gtcctgattt ttgttaatcc actataatca ggaaagacaa aaaaccttcc
       61 gccgtcattc ccgcgcaggc gggaatccga tccgttggga ttttgcaact tcaaatcaat
      121 ccgcaaactg aaatcccgtc attcccgcgc agtcgtgaat ccgaacgcgt ccgcacggaa
      181 atccgcatcc cgtcattccc acgaaagtgg gaatctagaa tgtcaaatct caagaaaccg
      241 ttttatccga taagttccta tgccgacgga cc
//
LOCUS       DAXDSD010000286          270 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXDSD010000286 DAXDSD010000000
VERSION     DAXDSD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.290"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(150..>270)
                     /locus_tag="ACFB2Q_002253"
     CDS             complement(150..>270)
                     /locus_tag="ACFB2Q_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HFC0919178.1"
                     /translation="MFDLKRALGKFGREKKKRMESQRQAELEKVYGRRVILFK"
BASE COUNT           74 a           78 c           45 g           73 t
ORIGIN      
        1 caacgaaccg gattcccact ttcgagggaa tgacgaagtg gaagttaccc gaaacttaaa
       61 acaagcgaaa ccgaacggac tggattcccg cctgcgcggg aatgacagtg tgtccatttc
      121 tgattttaat cccctatatt ttacacaaac tatttgaaca atatgacccg cctgccgtaa
      181 actttctcaa gctccgcctg cctttgactc tccattcttt tcttcttttc cctaccgaat
      241 ttacccaaag cacgcttcaa gtcaaacatc
//
LOCUS       DAXDSD010000287          268 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXDSD010000287 DAXDSD010000000
VERSION     DAXDSD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.291"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..137
                     /locus_tag="ACFB2Q_002254"
     CDS             <1..137
                     /locus_tag="ACFB2Q_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Imm41 family immunity protein"
                     /protein_id="HFC0919179.1"
                     /translation="IKASPWLPDSVGINERYERLKTMLRYIFTEKDIVNVQFDYYNKK
                     "
BASE COUNT           89 a           60 c           44 g           75 t
ORIGIN      
        1 aaattaaagc ttccccttgg ttacctgata gcgtgggaat taacgaacgt tatgaaaggc
       61 ttaaaacaat gctccgttat atttttaccg agaaagacat agtcaacgtg caatttgatt
      121 attacaacaa aaaatagcaa aacccggcaa tcaaaatgcc gtctgaaacc gtgtttaatt
      181 ttcaggcgac cttcccctta ttttaaacag gatattggga actaagttct tcaaaaatcc
      241 tacacctgct ctttccacgg cagcacct
//
LOCUS       DAXDSD010000288          265 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXDSD010000288 DAXDSD010000000
VERSION     DAXDSD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.292"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>265
                     /locus_tag="ACFB2Q_002255"
     CDS             <1..>265
                     /locus_tag="ACFB2Q_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFC0919180.1"
                     /translation="RGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQRAEAERE
                     AQALAARRKAEAEEAKHQAAELAHRQEAKRKAAESAKRKAEEEE"
BASE COUNT           94 a           69 c           78 g           24 t
ORIGIN      
        1 cgcggacacc gttcggtaca gcaaaacaat gtggaaattg cccaagccca agcagaactt
       61 gcgcgccgac agcaggaaga acgcaaagcg gcagagttgt tggcaaaaca aagggcagaa
      121 gccgaacgtg aggctcaagc attggctgcg cgccgaaaag ccgaagccga agaggcaaaa
      181 caccaagctg ccgaacttgc acatcgccag gaagcaaaac gcaaagcggc agaatccgcg
      241 aaacgaaaag ctgaggaaga agagc
//
LOCUS       DAXDSD010000289          257 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXDSD010000289 DAXDSD010000000
VERSION     DAXDSD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.293"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..>257)
                     /locus_tag="ACFB2Q_002256"
     CDS             complement(<1..>257)
                     /locus_tag="ACFB2Q_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HFC0919181.1"
                     /translation="EHCGTAGYIISREAMRFFLDRFAVLPAERIKAVDLMMFTYFFDK
                     EGMPVYQVSPALCTQELHYAKFLSQNSMLGSDLEKDREQGR"
BASE COUNT           78 a           73 c           41 g           65 t
ORIGIN      
        1 ttcttccttg ttccctatct ttttccaaat cgctacccaa catactgttt tgactgagaa
       61 acttggcata atgcaattct tgggtacata aggcgggact aacctgataa acaggcatcc
      121 cctccttatc aaagaaataa gtaaacatca tcaaatctac cgctttaatc cgctctgccg
      181 gcaaaacggc aaacctgtcc aagaaaaacc gcatcgcctc acgcgaaatg atatagccag
      241 ccgtcccaca atgttcg
//
LOCUS       DAXDSD010000290          255 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXDSD010000290 DAXDSD010000000
VERSION     DAXDSD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.294"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           67 a           37 c           76 g           75 t
ORIGIN      
        1 gatggttgtt ggaatgaaaa aatttcagac ggcacgacga tgccgtctga aaatcggtgc
       61 ggattatagc acgatgtggg tttgggaggc aaaatattgt tttaaaatat gaatttaatc
      121 ggtcggaacg gctgtataat gtttggcttt aacgggaggt gtgtgtatgg gcggcattgc
      181 tgctgtgtgc gtgtaccagc aatttcggcg acagggaaca tcagttcctg cgttatagtg
      241 gattaaattt aaacc
//
LOCUS       DAXDSD010000291          252 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXDSD010000291 DAXDSD010000000
VERSION     DAXDSD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.295"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           48 a           72 c           86 g           46 t
ORIGIN      
        1 tatgtgcaaa cgcttaaaat cattgaaaaa cctgtgtacc gcaatgcctg ttttgatgct
       61 gacggcgtgc gcgaactcaa cgccgccgtt gacgacggcg gttaagccgc ccgccgattt
      121 ggtgcggccc tgcccgaaac tgccgcacct tgaagggaac acgggcgcgg acgtgctgcc
      181 gtgggccctg aaggcggccg gtatgtataa cgactgcagg gcgcggcacg gcgcgctggt
      241 acgggcgttg gg
//
LOCUS       DAXDSD010000292          239 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXDSD010000292 DAXDSD010000000
VERSION     DAXDSD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.296"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            1..239
                     /locus_tag="ACFB2Q_002257"
                     /pseudo
     CDS             1..239
                     /locus_tag="ACFB2Q_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776296.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           49 a           68 c           70 g           52 t
ORIGIN      
        1 gcaatgggac atcggcggca gcggggcggt tttcccttcg ctcgcactgt ttctgctctg
       61 tttcatcata ggtatgcaca acacggggat gacgcttctg ccgggcggtg caatctgttc
      121 gacgcacatg gcccggcacg gcagccgact tgggcatcga aatcccgcgc gtgccgtact
      181 atagtggatt aacaaaaacc agtacggcgt tggcccgcct tagctcaaag agaacgatt
//
LOCUS       DAXDSD010000293          236 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXDSD010000293 DAXDSD010000000
VERSION     DAXDSD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.297"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           46 a           60 c           69 g           61 t
ORIGIN      
        1 gcggtttcgg gaggcgccgt ttgcggtttc gcagggtgcg gtccggcagg ggcgcgcgcg
       61 ccgcgcgcat ttgatcctga cggttctact gacggttcta ctgacggttc tacatataag
      121 gaaccgtcaa aatctgatgg ttcatcgctc aaaatctgat ggttcttagc gcaaaatctg
      181 acggttctta gcgcaaaatc cgacggttcg ccgctcaaaa tctgatggtt cttagc
//
LOCUS       DAXDSD010000294          232 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXDSD010000294 DAXDSD010000000
VERSION     DAXDSD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.298"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..205
                     /locus_tag="ACFB2Q_002258"
     CDS             <1..205
                     /locus_tag="ACFB2Q_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919182.1"
                     /translation="RRISDVRRPMMKMLVADKGGALVRAVKDSEDVIRHCDNGAGFSC
                     FNWTEHFKRTANLISLMHREAAA"
BASE COUNT           56 a           66 c           73 g           37 t
ORIGIN      
        1 ccgacggata agcgacgtgc gccggccgat gatgaaaatg cttgttgcgg acaaaggcgg
       61 cgcgttggtc cgggccgtaa aagactcgga agacgtcatc cggcattgcg acaacggcgc
      121 cggcttcagc tgcttcaact ggaccgaaca tttcaaacgg acggcaaacc tgatcagcct
      181 catgcacagg gaagcggcgg catgaggacg cacatccgga cctgcgtgta tc
//
LOCUS       DAXDSD010000295          225 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXDSD010000295 DAXDSD010000000
VERSION     DAXDSD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.299"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           67 a           50 c           67 g           41 t
ORIGIN      
        1 gcagtattca agccctatct gtggagcttg ctaggtatat cggcggcagg gttgatagtc
       61 atagcagggc tgttcatagc gatatggagc gtcaagaacg agctggacga cttgaaacag
      121 cagagagccg aagcagagcg caccctagac ctgttggaaa ccaagaccaa aggtttgaca
      181 ctggaaaatt gcccagtcga gaacagcaaa gcaacgcggg tatgc
//
LOCUS       DAXDSD010000296          224 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXDSD010000296 DAXDSD010000000
VERSION     DAXDSD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.300"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..>224)
                     /locus_tag="ACFB2Q_002259"
     CDS             complement(<1..>224)
                     /locus_tag="ACFB2Q_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HFC0919183.1"
                     /translation="VTGCKPVTARYGNPYRETVYIEVSDGIGNSQTLISNRIHSFYSG
                     GKWIKAEDLKAGSRLLSESGRTQTVRKTVV"
BASE COUNT           44 a           54 c           59 g           67 t
ORIGIN      
        1 ttgacaacgg ttttgcggac ggtttgggtt ctgccgcttt cggataacag tctgcttccc
       61 gcttttaaat cttccgcctt aatccatttg ccgcccgaat aaaacgagtg gatgcggttg
      121 gaaatcaggg tttggctgtt gccgatgccg tctgaaactt caatgtaaac ggtttcccga
      181 tacggattgc cgtatcgggc ggtaacaggt ttgcatcccg ttac
//
LOCUS       DAXDSD010000297          222 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXDSD010000297 DAXDSD010000000
VERSION     DAXDSD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.301"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            26..>222
                     /locus_tag="ACFB2Q_002260"
     CDS             26..>222
                     /locus_tag="ACFB2Q_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919184.1"
                     /translation="MAEEMRTCKACGGTKPLEKGFNAVPRKEGGVYYYKSCKTCRNKA
                     VRQKRAEKRAAAGAGAMTAARL"
BASE COUNT           69 a           54 c           74 g           25 t
ORIGIN      
        1 cataaaaaca aaaggaaaaa tcaaaatggc ggaagaaatg cgcacctgca aggcctgcgg
       61 cgggaccaag ccgttggaga aagggtttaa tgccgtcccg cgcaaggaag ggggggtcta
      121 ttattacaaa tcgtgcaaaa cctgccgcaa caaggcagtc cggcaaaagc gcgcggaaaa
      181 acgcgcggcg gcgggagccg gcgcgatgac ggcggcaagg ct
//
LOCUS       DAXDSD010000298          222 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXDSD010000298 DAXDSD010000000
VERSION     DAXDSD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.302"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>222
                     /locus_tag="ACFB2Q_002261"
     CDS             <1..>222
                     /locus_tag="ACFB2Q_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC0919185.1"
                     /translation="LNIKHQKTERQENGTFHAVSSLGLSTIYDFDTGSRFKPYIGMRV
                     AYGHVRHQVHSMEKETTAVTTYPSNGGTPS"
BASE COUNT           65 a           66 c           43 g           48 t
ORIGIN      
        1 cttaacataa aacaccaaaa gacggaacgt caggaaaacg gcacattcca cgccgtttct
       61 tctctcggct tgtcaaccat ttacgatttc gataccggtt cccgcttcaa accctatatc
      121 ggcatgcgcg tcgcctacgg acacgtcaga catcaggttc attcaatgga aaaagaaacc
      181 acggctgtta ccacttaccc aagcaacggt ggtacgccaa gt
//
LOCUS       DAXDSD010000299          215 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXDSD010000299 DAXDSD010000000
VERSION     DAXDSD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.303"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>215
                     /locus_tag="ACFB2Q_002262"
     CDS             <1..>215
                     /locus_tag="ACFB2Q_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFC0919186.1"
                     /translation="EAPAYGCIAVKPRRGGGHVFFVVGKDAEGRILGLGGNQGNMVSI
                     IPFDPADIDGYFWPSKLIGGKAVPSSP"
BASE COUNT           39 a           67 c           66 g           43 t
ORIGIN      
        1 tcgaagcccc cgcatacggc tgcatcgcgg tcaaaccgcg ccggggcggc ggacacgtgt
       61 tcttcgttgt cggcaaagac gcggaaggca gaatcttggg cttgggcggc aatcagggca
      121 atatggtatc catcatcccg tttgaccctg cggacattga cggctacttc tggccgtcca
      181 agctgattgg cggcaaagcc gtgccttcgt ccccc
//
LOCUS       DAXDSD010000300          212 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXDSD010000300 DAXDSD010000000
VERSION     DAXDSD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.304"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(<1..>212)
                     /locus_tag="ACFB2Q_002263"
     CDS             complement(<1..>212)
                     /locus_tag="ACFB2Q_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919187.1"
                     /translation="TIRKYTDTDKCGAQTAILGINTADGGALTPRSARPIVPEANKDV
                     AQYSGHKTAGGKSVPIGCMWKNSKTV"
BASE COUNT           33 a           58 c           62 g           59 t
ORIGIN      
        1 cagacggttt tgctgttttt ccacatgcag cctatgggga cggacttgcc gccggctgtc
       61 ttatggccgg aatattgtgc gacatcctta ttggcttccg gcacaatcgg gcgcgcgctt
      121 ctcggagtca atgcgccgcc gtcggcggta ttgatgccca aaatggcggt ttgcgcgcca
      181 catttgtccg tatccgtata tttccggatg gt
//
LOCUS       DAXDSD010000301          212 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXDSD010000301 DAXDSD010000000
VERSION     DAXDSD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.305"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..>212
                     /locus_tag="ACFB2Q_002264"
     CDS             <1..>212
                     /locus_tag="ACFB2Q_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919188.1"
                     /translation="AGLKDCGFDGGTEAALLAVISAAYYIRDGEDNFAWAAACVEDLL
                     EKMPPEAGRAPHPESPMPPETAGGGI"
BASE COUNT           38 a           63 c           73 g           38 t
ORIGIN      
        1 cgccggtctg aaagattgcg ggttcgacgg cggcacggag gccgcgctgc ttgccgtcat
       61 ctccgccgct tattacatcc gcgacggcga agacaatttt gcgtgggcgg ccgcctgtgt
      121 cgaagatttg ttggaaaaaa tgccgcctga agcggggcgg gccccgcacc ctgaaagccc
      181 gatgccgcct gaaacggcgg gcggaggcat tt
//
LOCUS       DAXDSD010000302          212 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXDSD010000302 DAXDSD010000000
VERSION     DAXDSD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..212
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.306"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(10..>212)
                     /locus_tag="ACFB2Q_002265"
     CDS             complement(10..>212)
                     /locus_tag="ACFB2Q_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC0919189.1"
                     /translation="EVDYIYGADGCGFLPSNLTEFRKYCRKKHQKFKDDEVLVNRYAV
                     DFLGAKEPPYDDRHVTSVKYFV"
BASE COUNT           60 a           50 c           38 g           64 t
ORIGIN      
        1 ttggttttat caaacaaaat atttgactga agtcacatgg cggtcgtcat atgggggttc
       61 cttcgcaccc aaaaaatcga ccgcgtaacg gtttactaaa acttcatcgt ccttaaactt
      121 ttggtgcttt ttccggcaat attttctgaa ctccgttaaa tttgacggca agaacccgca
      181 accgtctgca ccgtaaatgt aatcaacttc aa
//
LOCUS       DAXDSD010000303          210 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXDSD010000303 DAXDSD010000000
VERSION     DAXDSD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.307"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           65 a           51 c           46 g           48 t
ORIGIN      
        1 cgatgaagca gtgcgacaac atagacaagg gcaaccttga ttcacactgc cataaccgct
       61 tgctgccaag gaaaacaaaa tgaatttgcc tattcaaaaa ttcatgatgc tgtttgcagc
      121 ggcaatatcg ttgctgcaaa tccccattag tcatgcgaac ggtttggatg cccgtttgcg
      181 cgatgatatg caggcaaaac actacgaacc
//
LOCUS       DAXDSD010000304          206 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXDSD010000304 DAXDSD010000000
VERSION     DAXDSD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.308"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            <1..140
                     /locus_tag="ACFB2Q_002266"
     CDS             <1..140
                     /locus_tag="ACFB2Q_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC0919190.1"
                     /translation="ANAALPETAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVM
                     K"
BASE COUNT           55 a           42 c           61 g           48 t
ORIGIN      
        1 aggcaaacgc agcgttgccc gaaacggcaa aaaacgcttt ggaagccgca aaagcggacg
       61 gtatggaagc cggttggatt gtagtgggcg ttttcgccgc gctttttgta ttttccatcg
      121 ttaagagggt gatgaagtaa ggcggcatgt actgccaagt cggaaataaa tgtcttgaga
      181 agcaccgggc cgaaaacctt tatttc
//
LOCUS       DAXDSD010000305          202 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXDSD010000305 DAXDSD010000000
VERSION     DAXDSD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.309"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            complement(85..>202)
                     /locus_tag="ACFB2Q_002267"
     CDS             complement(85..>202)
                     /locus_tag="ACFB2Q_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFC0919191.1"
                     /translation="KKKPKKVIIAALMRKLAVIAYHVHKKGGDYDPSRYKSA"
BASE COUNT           51 a           41 c           40 g           70 t
ORIGIN      
        1 attgaatttc aatatttgaa aattcaccgg atttcagacg cggcaaagca ggcatttaaa
       61 aaatgccttt tttcctttcg ggatttacgc cgatttgtaa cgcgatggat cgtaatctcc
      121 gcctttctta tgtacgtgat acgcaataac ggcgagttta cgcatcaatg ctgcgatgat
      181 gactttttta ggcttcttct tt
//
LOCUS       DAXDSD010000306          202 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXDSD010000306 DAXDSD010000000
VERSION     DAXDSD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.denovo.310"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
BASE COUNT           64 a           55 c           39 g           44 t
ORIGIN      
        1 ctataaaaaa caaaggtaga aacgcggcag gcaaacgcag gcacgtccga tttccgcgcc
       61 gccccataaa acagttgaca tcatatgaca tttaaattga taatcaaaac tattattaat
      121 acatcatcaa gattgagtat gcgatgaaat acaaagccct gcccttactg ccgcttgccg
      181 ccgcccttgc cgcctgtgcc gg
//
LOCUS       DAXDSD010000307         1089 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.guided.193, whole genome shotgun sequence.
ACCESSION   DAXDSD010000307 DAXDSD010000000
VERSION     DAXDSD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.guided.193"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            8..868
                     /gene="blaTEM"
                     /locus_tag="ACFB2Q_002268"
     CDS             8..868
                     /gene="blaTEM"
                     /locus_tag="ACFB2Q_002268"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TEM family class A beta-lactamase"
                     /protein_id="HFC0919192.1"
                     /translation="MSIQHFRVALITFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTTPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          289 a          250 c          253 g          297 t
ORIGIN      
        1 gaagagtatg agtattcaac attttcgtgt cgcccttatt accttttttg cggcattttg
       61 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      121 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      181 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggtgcggt
      241 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      301 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      361 agaattatgc agtgctgcca taaccatgag tgataacact gctgccaact tacttctgac
      421 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      481 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      541 cacgacgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      601 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      661 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      721 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      781 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      841 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      901 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      961 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag acccctatct
     1021 ataaactctt ggcttggttc taatccctct aaacgattat tatcaatagc cgctctaacc
     1081 gctttttct
//
LOCUS       DAXDSD010000308         1089 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.guided.194, whole genome shotgun sequence.
ACCESSION   DAXDSD010000308 DAXDSD010000000
VERSION     DAXDSD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.guided.194"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            8..868
                     /gene="blaTEM"
                     /locus_tag="ACFB2Q_002269"
     CDS             8..868
                     /gene="blaTEM"
                     /locus_tag="ACFB2Q_002269"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TEM family class A beta-lactamase"
                     /protein_id="HFC0919193.1"
                     /translation="MSIQHFRVALITFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          289 a          249 c          253 g          298 t
ORIGIN      
        1 gaagagtatg agtattcaac attttcgtgt cgcccttatt accttttttg cggcattttg
       61 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      121 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      181 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggtgcggt
      241 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      301 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      361 agaattatgc agtgctgcca taaccatgag tgataacact gctgccaact tacttctgac
      421 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      481 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      541 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      601 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      661 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      721 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      781 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      841 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      901 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      961 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag acccctatct
     1021 ataaactctt ggcttggttc taatccctct aaacgattat tatcaatagc cgctctaacc
     1081 gctttttct
//
LOCUS       DAXDSD010000309         1089 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.guided.195, whole genome shotgun sequence.
ACCESSION   DAXDSD010000309 DAXDSD010000000
VERSION     DAXDSD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.guided.195"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            8..868
                     /locus_tag="ACFB2Q_002270"
     CDS             8..868
                     /locus_tag="ACFB2Q_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-135"
                     /protein_id="HFC0919194.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTTPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          288 a          251 c          253 g          297 t
ORIGIN      
        1 gaagagtatg agtattcaac attttcgtgt cgcccttatt cccttttttg cggcattttg
       61 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      121 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      181 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggtgcggt
      241 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      301 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      361 agaattatgc agtgctgcca taaccatgag tgataacact gctgccaact tacttctgac
      421 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      481 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      541 cacgacgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      601 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      661 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      721 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      781 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      841 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      901 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      961 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag acccctatct
     1021 ataaactctt ggcttggttc taatccctct aaacgattat tatcaatagc cgctctaacc
     1081 gctttttct
//
LOCUS       DAXDSD010000310         1089 bp    DNA     linear   BCT 19-SEP-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae strain NG313
            SAMN08583479-rid23190153.guided.196, whole genome shotgun sequence.
ACCESSION   DAXDSD010000310 DAXDSD010000000
VERSION     DAXDSD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08583479
            Sequence Read Archive: SRR6765715
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002362856.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002362856.1
            Genome Coverage       :: 217x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/18/2024 01:33:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,271
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,062
            CDSs (with protein)               :: 2,062
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 103 of 160
            Pseudo Genes (incomplete)         :: 54 of 160
            Pseudo Genes (internal stop)      :: 44 of 160
            Pseudo Genes (multiple problems)  :: 40 of 160
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08583479-rid23190153.guided.196"
                     /strain="NG313"
                     /isolation_source="urethra"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /geo_loc_name="China: Guangdong"
                     /lat_lon="23.08 N 113.14 E"
                     /collection_date="2012-04-20"
                     /collected_by="NCSTD"
     gene            8..868
                     /locus_tag="ACFB2Q_002271"
     CDS             8..868
                     /locus_tag="ACFB2Q_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFC0919195.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          288 a          250 c          253 g          298 t
ORIGIN      
        1 gaagagtatg agtattcaac attttcgtgt cgcccttatt cccttttttg cggcattttg
       61 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
      121 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
      181 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggtgcggt
      241 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      301 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      361 agaattatgc agtgctgcca taaccatgag tgataacact gctgccaact tacttctgac
      421 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      481 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      541 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      601 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      661 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      721 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      781 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      841 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      901 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      961 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag acccctatct
     1021 ataaactctt ggcttggttc taatccctct aaacgattat tatcaatagc cgctctaacc
     1081 gctttttct
//
